title: Prediction of ocular irritancy of 26 chemicals and 26 cosmetic products with isolated rabbit eye (IRE) test.
abstract: OBJECTIVE
  This study aims to establish and evaluate the methodology of isolated rabbit eye (IRE) test.
  
  
  METHODS
  IRE test was performed according to modifications of the in vitro toxicology (INVITTOX) Protocol No.85: Rabbit enucleated eye test by European Centre for the Validation of Alternative Methods (ECVAM), and then 26 chemicals and 26 cosmetic products were tested in both in vitro IRE and in vivo Draize tests. A statistical analysis was conducted to determine the relevance of the IRE test to the data generated in the Draize test.
  
  
  RESULTS
  IRE test was established successfully in our laboratory. It was shown that ranking correlation and class concordance were fairly well between the IRE test and the Draize test for 26 reference chemicals (Fisher's Exact Test χ(2)=51.314, P<0.001; McNemar P=0.261; Gamma=0.960, P<0.001; Kappa=0.843, P<0.001) and 26 cosmetic products (Fisher's Exact Test χ(2)=15.522, P<0.001; McNemar P=0.311; Gamma=0.967, P<0.001; Kappa=0.611, P<0.001).
  
  
  CONCLUSIONS
  IRE test was established successfully for in vitro testing of eye irritation as an alternative to Draize test.
authors: Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 510300, China.
authors: Xiang
authors: X
authors: Guo
authors: Xing Fen
authors: XF
authors: Yang
authors: Ying
authors: Y
authors: Yang
authors: Raabe
authors: R
authors: Hans
authors: Jing Heng
authors: JH
authors: Cai
authors: Jin Yu
authors: JY
authors: Xue
authors: Xiao Hua
authors: XH
authors: Tan
authors: Xiao Ping
authors: XP
authors: Xie
authors: Xi Kun
authors: XK
authors: Xiong
authors: Jun Ming
authors: JM
authors: Huang
mesh_terms: D000826:Animal Testing Alternatives; D000818:Animals; D003358:Cosmetics / Q000633:toxicity*; D005123:Eye / Q000187:drug effects*; D007509:Irritants / Q000633:toxicity*; D011817:Rabbits; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D003358:Cosmetics; D007509:Irritants