title: Luteinizing hormone receptor splicing variants in bovine Leydig cells.
abstract: The luteinizing hormone receptor (LHR) plays a key  role in testosterone production through its interaction with the gonadotropins,  LH and chorionic gonadotropin. We examined the LHR splicing pattern in  bovine Leydig cells; LH-induced expression of eight cloned splicing variants  was detected by real-time PCR. Luteinizing hormone applied to cultured Leydig  cells resulted in expression of full-length LHR and the A and B isoforms, as  well as secretion of testosterone, which first increased, then declined, and then  increased further, with increased LH levels. The secretion of testosterone  progressively increased with increasing LH, but the expression levels of LHR  (FL, A, and B) did not increase correspondingly. We conclude that the LHR  splicing pattern is complex in bovine Leydig cells, and that expression of  full-length LHR and isoforms A and B changes when induced with LH.
authors: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
authors: T H
authors: TH
authors: Ma
authors: Q H
authors: QH
authors: Xiong
authors: B
authors: B
authors: Yuan
authors: H
authors: H
authors: Jiang
authors: Y
authors: Y
authors: Gao
authors: J B
authors: JB
authors: Xu
authors: S Y
authors: SY
authors: Liu
authors: Y
authors: Y
authors: Ding
authors: G L
authors: GL
authors: Zhang
authors: Y M
authors: YM
authors: Zhao
authors: J B
authors: JB
authors: Zhang
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005091:Exons; D015870:Gene Expression; D007985:Leydig Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011974:Receptors, LH / Q000235:genetics / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D011974:Receptors, LH; D013739:Testosterone; D007986:Luteinizing Hormone