title: Mass spectrometry tools for analysis of intermolecular interactions.
abstract: The small quantities of protein required for mass spectrometry (MS) make it a powerful tool to detect binding (protein-protein, protein-small molecule, etc.) of proteins that are difficult to express in large quantities, as is the case for many intrinsically disordered proteins. Chemical cross-linking, proteolysis, and MS analysis, combined, are a powerful tool for the identification of binding domains. Here, we present a traditional approach to determine protein-protein interaction binding sites using heavy water ((18)O) as a label. This technique is relatively inexpensive and can be performed on any mass spectrometer without specialized software.
authors: Department of Biochemistry and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA, USA. jared.auclair@gmail.com
authors: Jared R
authors: JR
authors: Auclair
authors: Mohan
authors: M
authors: Somasundaran
authors: Karin M
authors: KM
authors: Green
authors: James E
authors: JE
authors: Evans
authors: Celia A
authors: CA
authors: Schiffer
authors: Dagmar
authors: D
authors: Ringe
authors: Gregory A
authors: GA
authors: Petsko
authors: Jeffrey N
authors: JN
authors: Agar
mesh_terms: D002853:Chromatography, Liquid; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D013058:Mass Spectrometry / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D003432:Cross-Linking Reagents; D010455:Peptides; D011506:Proteins