title: Isolation and characteristics of eight novel polymorphic microsatellite loci in Lippia alba (Verbenaceae).
abstract: OBJECTIVE
  A set of eight microsatellite (simple sequence repeat [SSR]) markers for Lippia alba, an important medicinal and cosmetic plant, was developed to aid studies of genetic diversity and to define efficient strategies for breeding programs.
  
  
  RESULTS
  Using a (CT)(8)- and (GT)(8)-enriched library, a total of 11 SSR loci were developed and optimized in L. alba. Of the 11 loci, eight were found to be polymorphic after screening 61 accessions from two populations. The parameters used to characterize loci were expected heterozygosity (H(e)) and number of alleles. A total of 44 alleles were identified, with an average of 5.5 alleles per loci, which were moderately to highly informative according to H(e).
  
  
  CONCLUSIONS
  These new SSR markers have potential for informing genetic diversity, allele mining, and mapping studies and will be used to generate information for breeding programs of L. alba.
authors: Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, Instituto Agronômico, Campinas, São Paulo, Brazil.
authors: Fernanda R C
authors: FR
authors: Santos
authors: Paula F
authors: PF
authors: Lima
authors: Regina H G
authors: RH
authors: Priolli
authors: Walter J
authors: WJ
authors: Siqueira
authors: Carlos A
authors: CA
authors: Colombo
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015723:Gene Library; D056426:Genetic Loci; D005819:Genetic Markers; D032411:Lippia / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000145:classification / Q000235:genetics; D010946:Plants, Medicinal; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers