title: Pullularins E and F, two new peptides from the endophytic fungus Bionectria ochroleuca isolated from the mangrove plant Sonneratia caseolaris.
abstract: Chemical investigation of the EtOAc extract of the endophytic fungus Bionectria ochroleuca, isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan island (China), yielded two new peptides, pullularins E and F (1 and 2) together with three known compounds (3-5). The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configurations of amino acids were determined by HPLC analysis of acid hydrolysates using Marfey's method. The isolated compounds exhibited pronounced to moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC₅₀ values ranging between 0.1 and 6.7 µg/mL.
authors: Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine University, Universitaetsstrasse 1, D-40225 Duesseldorf, Germany.|Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
authors: Weaam
authors: W
authors: Ebrahim
authors: Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine University, Universitaetsstrasse 1, D-40225 Duesseldorf, Germany.
authors: Julia
authors: J
authors: Kjer
authors: Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine University, Universitaetsstrasse 1, D-40225 Duesseldorf, Germany.
authors: Mustapha
authors: M
authors: El Amrani
authors: Helmholtz Centre for Infection Research, Inhoffenstraße 7, Braunschweig D-38124, Germany.
authors: Victor
authors: V
authors: Wray
authors: National Research Laboratories of Natural and Biomimetic Drugs, Health Science Center, Peking University, Beijing 100083, China.
authors: Wenhan
authors: 0000-0002-4978-4083
authors: W
authors: Lin
authors: Marine Biodiscovery Centre, University of Aberdeen, Meston Walk, Aberdeen, Scotland AB24 3UE, UK.
authors: Rainer
authors: 0000-0002-9702-2235
authors: R
authors: Ebel
authors: Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine University, Universitaetsstrasse 1, D-40225 Duesseldorf, Germany.
authors: Daowan
authors: D
authors: Lai
authors: Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine University, Universitaetsstrasse 1, D-40225 Duesseldorf, Germany.
authors: Peter
authors: P
authors: Proksch
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D019669:Embryophyta / Q000382:microbiology*; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D006999:Hypocreales / Q000737:chemistry* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000382:microbiology*
chemical_list: D005656:Fungal Proteins; D010455:Peptides