title: Novel antibodies reacting with two neighboring gangliosides are induced in rabbits immunized with bovine brain gangliosides.
abstract: Immunization of rabbits with bovine brain gangliosides induced an experimental neuropathy, with clinical signs resembling Guillain-Barré syndrome. All the immunized animals developed immunoglobulin G immunoreactivity to GM1 ganglioside. In a few (4 of 27) animals, an additional anti-ganglioside antibody population showing an unusual binding behavior was detected. Enzyme-linked immunosorbent assay and thin-layer chromatography immunostaining analyses showed that the binding of these unusual antibodies required the presence of two co-localized gangliosides. Maximal interaction was observed to a mixture of GM1 and GD1b, but the antibodies also showed "density-dependent" binding to GD1b. The antibodies were purified by affinity chromatography and displayed the ability to target antigens in biological membranes (rat synaptosomes).
authors: Departamento de Química Biológica Dr. Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba and CIQUIBIC, CONICET, Córdoba 5000, Argentina.
authors: Ana L
authors: AL
authors: Moyano
authors: Romina
authors: R
authors: Comín
authors: Aldo A
authors: AA
authors: Vilcaes
authors: Samanta C
authors: SC
authors: Funes
authors: Germán A
authors: GA
authors: Roth
authors: Fernando J
authors: FJ
authors: Irazoqui
authors: Gustavo A
authors: GA
authors: Nores
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D005677:G(M1) Ganglioside / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology*; D020275:Guillain-Barre Syndrome / Q000139:chemically induced / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D009444:Neuritis, Autoimmune, Experimental / Q000139:chemically induced / Q000276:immunology; D011817:Rabbits; D051381:Rats
chemical_list: D005732:Gangliosides; D007074:Immunoglobulin G; C033851:ganglioside, GD1b; D005677:G(M1) Ganglioside