title: Optimization of hydrolysis conditions for the production of angiotensin-I converting enzyme-inhibitory peptides and isolation of a novel peptide from lizard fish (Saurida elongata) muscle protein hydrolysate.
abstract: Lizard fish (Saurida elongata) muscle protein was hydrolyzed using neutral protease to produce protein hydrolysate (LFPH), and the hydrolysis conditions were investigated using response-surface methodology. The optimum conditions for producing peptides with the highest angiotensin-I converting enzyme (ACE)-inhibitory activity were the following: enzyme-to-substrate ratio of 10,000 U/g, temperature of 48 °C, pH 7.0, and hydrolysis time of 2 h. Under these conditions, the ACE-inhibitory activity of LFPH and the degree of hydrolysis were 84% and 24%, respectively. A novel ACE-inhibitory peptide was isolated from LFPH using ultrafiltration, Sephadex G-15, and high-performance liquid chromatography. The amino acid sequence of the ACE-inhibitory peptide was identified as Ser-Pro-Arg-Cys-Arg (SPRCR), and its IC₅₀ was 41 ± 1 µM.
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, China.|Department of Pharmacy, Liuzhou Medical College, Liuzhou, Guangxi 545006, China.
authors: Shanguang
authors: S
authors: Wu
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, China.|Guangxi Key Laboratory of Petrochemical Resources Processing & Process Intensification Technology, Nanning, Guangxi 530004, China.
authors: Jianhua
authors: J
authors: Sun
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, China.|Guangxi Key Laboratory of Petrochemical Resources Processing & Process Intensification Technology, Nanning, Guangxi 530004, China.
authors: Zhangfa
authors: Z
authors: Tong
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, China.
authors: Xiongdiao
authors: X
authors: Lan
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, China.
authors: Zhongxing
authors: Z
authors: Zhao
authors: School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, China.|Guangxi Key Laboratory of Petrochemical Resources Processing & Process Intensification Technology, Nanning, Guangxi 530004, China.
authors: Dankui
authors: D
authors: Liao
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005399:Fishes / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009124:Muscle Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013696:Temperature
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D009124:Muscle Proteins; D010455:Peptides; D011492:Protein Hydrolysates; D010447:Peptide Hydrolases