title: Proteomic analysis of midtrimester amniotic fluid to identify novel biomarkers for preterm delivery.
abstract: OBJECTIVE
  The aim of this study was to identify possible biomarkers for preterm delivery by analyzing midtrimester amniotic fluid.
  
  
  METHODS
  Thirty-two amniotic fluid samples were studied; 16 patients had a spontaneous preterm delivery and 16 patients delivered at term. The proteomic technique consisted of surface-enhanced laser desorption ionization time-of-flight (SELDI-TOF) using different types of solid chromatographic chips (Q10, CM10 and IMAC30).
  
  
  RESULTS
  Mass spectrometry tracings were obtained from the amniotic fluids of both patients who delivered preterm and patients who delivered at term. Seven potential markers were identified to be differentially expressed in patients who delivered preterm.
  
  
  CONCLUSIONS
  Proteomic analysis of amniotic fluid obtained in the midtrimester reveals the presence of a set of proteins in patients at risk for preterm delivery.
authors: Department of Gynecology, Berlin Centrum for Regenerative Therapies, Charité-University Hospital, Berlin, Germany.
authors: Christina
authors: C
authors: Fotopoulou
authors: Sarah
authors: S
authors: Kyeyamwa
authors: Mattea
authors: M
authors: Linder
authors: Daniela
authors: D
authors: Thieme
authors: Sebastian
authors: S
authors: Hartenstein
authors: Oliver
authors: O
authors: Klein
authors: Joachim W
authors: JW
authors: Dudenhausen
authors: Wolfgang
authors: W
authors: Henrich
authors: Karim D
authors: KD
authors: Kalache
authors: Christian
authors: C
authors: Bamberg
mesh_terms: D000328:Adult; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000737:chemistry* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D036861:Delivery, Obstetric; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000097:blood / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D011247:Pregnancy; D011262:Pregnancy Trimester, Second / Q000378:metabolism; D047928:Premature Birth / Q000175:diagnosis* / Q000209:etiology / Q000378:metabolism; D011379:Prognosis; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012307:Risk Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D020543:Proteome