title: Life's attractors : understanding developmental systems through reverse engineering and in silico evolution.
abstract: We propose an approach to evolutionary systems biology which is based on reverse engineering of gene regulatory networks and in silico evolutionary simulations. We infer regulatory parameters for gene networks by fitting computational models to quantitative expression data. This allows us to characterize the regulatory structure and dynamical repertoire of evolving gene regulatory networks with a reasonable amount of experimental and computational effort. We use the resulting network models to identify those regulatory interactions that are conserved, and those that have diverged between different species. Moreover, we use the models obtained by data fitting as starting points for simulations of evolutionary transitions between species. These simulations enable us to investigate whether such transitions are random, or whether they show stereotypical series of regulatory changes which depend on the structure and dynamical repertoire of an evolving network. Finally, we present a case study-the gap gene network in dipterans (flies, midges, and mosquitoes)-to illustrate the practical application of the proposed methodology, and to highlight the kind of biological insights that can be gained by this approach.
authors: Universitat Pompeu Fabra, Barcelona, Spain. yogi.jaeger@crg.eu
authors: Johannes
authors: J
authors: Jaeger
authors: Anton
authors: A
authors: Crombach
mesh_terms: D000465:Algorithms; D000818:Animals; D005075:Biological Evolution; D003198:Computer Simulation; D004175:Diptera / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D005818:Genetic Engineering / Q000379:methods*; D005838:Genotype; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008954:Models, Biological; D010641:Phenotype; D049490:Systems Biology
chemical_list: D019476:Insect Proteins