title: Resveratrol promotes cellular glucose utilization in primary cultured cortical neurons via calcium-dependent signaling pathway.
abstract: OBJECTIVE
  Impairment of glucose utilization contributes to neuronal degeneration of Alzheimer's disease patients. Cellular glucose utilization can be regulated by calcium-dependent signaling pathways. Resveratrol (RSV) is a plant-derived polyphenol with multiple beneficial effects, including neuroprotection and metabolic improvement. Here, we investigated the effect of RSV on neuronal calcium signal and glucose utilization.
  
  
  METHODS
  Primary culture of cortical neurons, calcium imaging, 2-NBDG assay and western blotting were employed to investigate RSV-mediated effects on neuronal calcium signal and glucose utilization.
  
  
  RESULTS
  RSV elevated intracellular calcium in cortical neurons via modulation of secondary messenger system including nitrous oxide, cGMP and cAMP. Secondarily, a calcium-dependent enhancement of neuronal glucose utilization after RSV treatment was observed. The effects on neuronal glucose utilization are largely dependent on RSV-induced calcium-dependent AMP-activated protein kinase activation.
  
  
  CONCLUSIONS
  Our findings show that activation of calcium-dependent signaling pathways by RSV may convey improvements of neuronal glucose utilization.
authors: Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors: Jun-Qi
authors: JQ
authors: Zhang
authors: Peng-Fei
authors: PF
authors: Wu
authors: Li-Hong
authors: LH
authors: Long
authors: Yu
authors: Y
authors: Chen
authors: Zhuang-Li
authors: ZL
authors: Hu
authors: Lan
authors: L
authors: Ni
authors: Fang
authors: F
authors: Wang
authors: Jian-Guo
authors: JG
authors: Chen
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D013267:Stilbenes; D005947:Glucose; D000077185:Resveratrol; D002118:Calcium