title: Characterization of a novel HMG-CoA lyase enzyme with a dual location in endoplasmic reticulum and cytosol.
abstract: A novel lyase activity enzyme is characterized for the first time: HMG-CoA lyase-like1 (er-cHL), which is a close homolog of mitochondrial HMG-CoA lyase (mHL). Initial data show that there are nine mature transcripts for the novel gene HMGCLL1, although none of them has all its exons. The most abundant transcript is called "variant b," and it lacks exons 2 and 3. Moreover, a three-dimensional model of the novel enzyme is proposed. Colocalization studies show a dual location of the er-cHL in the endoplasmic reticulum (ER) and cytosol, but not in mitochondria or peroxisomes. Furthermore, the dissociation experiment suggests that it is a nonendoplasmic reticulum integral membrane protein. The kinetic parameters of er-cHL indicate that it has a lower V(max) and a higher substrate affinity than mHL. Protein expression and lyase activity were found in several tissues, and were particularly strong in lung and kidney. The occurrence of er-cHL in brain is surprising, as mHL has not been found there. Although mHL activity is clearly associated with energy metabolism, the results suggest that er-cHL is more closely related to another metabolic function, mostly at the pulmonary and brain level.
authors: Unit of Clinical Genetics and Functional Genomics, Department of Pharmacology and Physiology, School of Medicine, University of Zaragoza, Spain.
authors: María
authors: M
authors: Arnedo
authors: Unit of Clinical Genetics and Functional Genomics, Department of Pharmacology and Physiology, School of Medicine, University of Zaragoza, Spain.
authors: Sebastián
authors: S
authors: Menao
authors: Unit of Clinical Genetics and Functional Genomics, Department of Pharmacology and Physiology, School of Medicine, University of Zaragoza, Spain.
authors: Beatriz
authors: B
authors: Puisac
authors: Unit of Clinical Genetics and Functional Genomics, Department of Pharmacology and Physiology, School of Medicine, University of Zaragoza, Spain.
authors: María E
authors: ME
authors: Teresa-Rodrigo
authors: Unit of Clinical Genetics and Functional Genomics, Department of Pharmacology and Physiology, School of Medicine, University of Zaragoza, Spain.
authors: María C
authors: MC
authors: Gil-Rodríguez
authors: Molecular Modeling Group, Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Madrid, Spain; Biomol-Informatics SL, Parque Científico de Madrid, Spain.
authors: Eduardo
authors: E
authors: López-Viñas
authors: Molecular Modeling Group, Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Madrid, Spain.
authors: Paulino
authors: P
authors: Gómez-Puertas
authors: Basic Sciences Department, Universitat Internacional de Catalunya, Catalunya, Spain.
authors: Nuria
authors: N
authors: Casals
authors: Department of Molecular Biology, National University of Río Cuarto, Río Cuarto, Argentina; and.
authors: César H
authors: CH
authors: Casale
authors: Department of Biochemistry, School of Pharmacy, University of Barcelona, Barcelona, Spain.
authors: Fausto G
authors: FG
authors: Hegardt
authors: Unit of Clinical Genetics and Functional Genomics, Department of Pharmacology and Physiology, School of Medicine, University of Zaragoza, Spain. Electronic address: juanpie@unizar.es.
authors: Juan
authors: J
authors: Pié
mesh_terms: D000595:Amino Acid Sequence; D003600:Cytosol / Q000201:enzymology* / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D008969:Molecular Sequence Data; D007652:Oxo-Acid-Lyases / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D020675:Peroxisomes / Q000201:enzymology / Q000378:metabolism; D019154:Protein Splicing
chemical_list: D007652:Oxo-Acid-Lyases; C021410:3-hydroxy-3-methylglutaryl-coenzyme A lyase; C576753:HMGCLL1 protein, human