title: Black tea polyphenols reverse epithelial-to-mesenchymal transition and suppress cancer invasion and proteases in human oral cancer cells.
abstract: Epithelial-to-mesenchymal transition (EMT) in cancer cells is considered to be a prerequisite for acquiring invasive/migratory phenotype and subsequent metastasis. This study provides molecular evidence associated with the antimetastatic effect of black tea polyphenol extracts (BTE), which contain polyphenols including gallic acid, gallocatechin, catechin, epigallocatechin-3-gallate, epicatechin-3-gallate, and theaflavin 3,3'-digallate, in an an oral squamous cell culture system by showing a nearly complete inhibition on the invasion (p < 0.001) of SCC-4 cells via reduced activities of MMP-2 (p < 0.001) and u-PA (p < 0.001). Immunoblot was performed to find that BTE could induce up-regulation of epithelial markers such as E-cadherin and inhibit mesenchymal markers such as snail-1 and vimentin. BTE inhibited p-FAK and p-paxillin, indicating the anti-EMT effect of BTE in oral squamous cell carcinoma. BTE was evidenced by its inhibition of the tumor growth of SCC-4 cells via cancer cell xenografted nude mice mode. These results suggested that BTE could reduce invasion by reversing EMT in human oral cancer cells.
authors: Department of Dentistry and ‡ Clinical Laboratory, Chung Shan Medical University Hospital , No. 110, Section 1, Jianguo N. Road, Taichung, Taiwan.
authors: Yu-Chao
authors: YC
authors: Chang
authors: Pei-Ni
authors: PN
authors: Chen
authors: Shu-Chen
authors: SC
authors: Chu
authors: Chin-Yin
authors: CY
authors: Lin
authors: Wu-Hsien
authors: WH
authors: Kuo
authors: Yih-Shou
authors: YS
authors: Hsieh
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D028241:Camellia sinensis / Q000737:chemistry*; D002465:Cell Movement / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D018307:Neoplasms, Squamous Cell / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D059808:Polyphenols / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011480:Protease Inhibitors / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000235:genetics / Q000378:metabolism; D014746:Vimentin / Q000037:antagonists & inhibitors / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D015820:Cadherins; D059808:Polyphenols; D011480:Protease Inhibitors; D014746:Vimentin; D014568:Urokinase-Type Plasminogen Activator; D020778:Matrix Metalloproteinase 2