title: Pilt is a coiled-coil domain-containing protein that localizes at the trans-Golgi complex and regulates its structure.
abstract: Protein incorporated later into tight junctions (Pilt), also termed tight junction-associated protein 1 or tight junction protein 4, is a coiled-coil domain-containing protein that was originally identified as a human discs large-interacting protein. In this study, we identified Pilt as an Arf6-binding protein by yeast two-hybrid screening. By immunocytochemical analysis, Pilt was shown to be predominantly localized at the trans-Golgi complex and to exhibit diffuse cytoplasmic distribution in association with endosomes and plasma membrane in NIH3T3 cells. Silencing of endogenous Pilt disrupted the Golgi structure. The present findings suggest the functional involvement of Pilt in the maintenance of the Golgi structure.
authors: Department of Anatomy, Kitasato University School of Medicine, Sagamihara 252-0374, Japan.
authors: Hideaki
authors: H
authors: Tamaki
authors: Masashi
authors: M
authors: Sanda
authors: Osamu
authors: O
authors: Katsumata
authors: Yoshinobu
authors: Y
authors: Hara
authors: Masahiro
authors: M
authors: Fukaya
authors: Hiroyuki
authors: H
authors: Sakagami
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D055785:Gene Knockdown Techniques; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D062725:Tight Junction Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D020798:Two-Hybrid System Techniques; D021601:trans-Golgi Network / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D000089982:ADP-Ribosylation Factor 6; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; C445327:TJAP1 protein, human; D062725:Tight Junction Proteins; D004247:DNA; D020727:ADP-Ribosylation Factors; C000715348:ARF6 protein, human; C000715727:Arf6 protein, mouse