title: Antitumor and immunomodulatory activity of water-soluble polysaccharide from Inonotus obliquus.
abstract: The medicinal mushroom Inonotus obliquus has been used as a folk remedy for a long time in Russia and East-European countries to treat gastrointestinal cancer, cardiovascular disease and diabetes. In our study, a water-soluble polysaccharide (ISP2a) was successfully purified from I. obliquus by DEAE-Sepharose CL-6B and Sepharose CL-6B column chromatography. In vivo ISP2a had not only shown antitumor activity, but also could significantly enhance the immune response of tumor-bearing mice. In addition, ISP2a significantly enhanced the lymphocyte proliferation and increased the production of TNF-α. Results of these studies demonstrated that ISP2a had a potential application as natural antitumor agent with immunomodulatory activity.
authors: State Key Laboratory of Dairy Biotechnology, Technology Center of Bright Dairy & Food Co. Ltd., Shanghai 200436, China. fanliuping@jiangnan.edu.cn
authors: Liuping
authors: L
authors: Fan
authors: Shaodong
authors: S
authors: Ding
authors: Lianzhong
authors: L
authors: Ai
authors: Kequan
authors: K
authors: Deng
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D002277:Carcinoma / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008970:Molecular Weight; D012995:Solubility; D013274:Stomach Neoplasms / Q000473:pathology; D014867:Water / Q000737:chemistry / Q000494:pharmacology; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides; D007155:Immunologic Factors; D014867:Water