title: Regional distribution of calretinin and calbindin-D28k expression in the brain of the urodele amphibian Pleurodeles waltl during embryonic and larval development.
abstract: The sequence of appearance of calretinin and calbindin-D28k immunoreactive (CRir and CBir, respectively) cells and fibers has been studied in the brain of the urodele amphibian Pleurodeles waltl. Embryonic, larval and juvenile stages were studied. The early expression and the dynamics of the distribution of CBir and CRir structures have been used as markers for developmental aspects of distinct neuronal populations, highlighting the accurate extent of many regions in the developing brain, not observed on the basis of cytoarchitecture alone. CR and, to a lesser extent, CB are expressed early in the central nervous system and show a progressively increasing expression from the embryonic stages throughout the larval life and, in general, the labeled structures in the developing brain retain their ability to express these proteins in the adult brain. The onset of CRir cells primarily served to follow the development of the olfactory bulbs, subpallium, thalamus, alar hypothalamus, mesencephalic tegmentum, and distinct cell populations in the rhombencephalic reticular formation. CBir cells highlighted the development of, among others, the pallidum, hypothalamus, dorsal habenula, midbrain tegmentum, cerebellum, and central gray of the rostral rhombencephalon. However, it was the relative and mostly segregated distribution of both proteins in distinct cell populations which evidenced the developing regionalization of the brain. The results have shown the usefulness in neuroanatomy of the analysis during development of the onset of CBir and CRir structures, but the comparison with previous data has shown extensive variability across vertebrate classes. Therefore, one should be cautious when comparing possible homologue structures across species only on the basis of the expression of these proteins, due to the variation of the content of calcium-binding proteins observed in well-established homologous regions in the brain of different vertebrates.
authors: Departamento de Biología Celular, Facultad de Biología, Universidad Complutense, 28040 Madrid, Spain.
authors: Alberto
authors: A
authors: Joven
authors: Ruth
authors: R
authors: Morona
authors: Nerea
authors: N
authors: Moreno
authors: Agustín
authors: A
authors: González
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology* / Q000254:growth & development* / Q000378:metabolism; D064032:Calbindin 2 / Q000378:metabolism*; D064026:Calbindins / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007150:Immunohistochemistry; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008856:Microscopy, Fluorescence; D012455:Salamandridae
chemical_list: D064032:Calbindin 2; D064026:Calbindins