title: Technical and practical aspects of (19) F NMR-based screening: toward sensitive high-throughput screening with rapid deconvolution.
abstract: The technical and practical aspects of (19) F NMR-based screening against a macromolecular target are analyzed in detail. A novel method utilizing the relaxation of (19) F homonuclear double quantum coherence is proposed for performing NMR-based binding assays in a direct- or competition-mode format. A combined strategy based on (19) F NMR chemical shift prediction, 2D (19) F NMR DOSY, and 2D (19) F-(1) H NMR long-range COSY experiments is presented for the deconvolution of complex mixtures of fluorinated molecules generated by either addition of single compounds or by chemical synthesis. The approaches presented here allow the screening of complex mixtures, even in the case where the exact composition is not known, and the rapid identification of the binders contained in the mixtures.
authors: Department of Chemistry, University of Neuchâtel, CH 2000, Neuchâtel, Switzerland. claudio.dalvit@unine.ch
authors: Claudio
authors: C
authors: Dalvit
authors: Anna
authors: A
authors: Vulpetti
mesh_terms: D000818:Animals; D002417:Cattle; D005461:Fluorine / Q000737:chemistry; D057166:High-Throughput Screening Assays; D006845:Hydrocarbons, Fluorinated / Q000737:chemistry / Q000494:pharmacology; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D010179:Pancreas / Q000201:enzymology; D014357:Trypsin / Q000378:metabolism
chemical_list: D006845:Hydrocarbons, Fluorinated; D008024:Ligands; D005461:Fluorine; D014357:Trypsin