title: Histone H2B monoubiquitination facilitates the rapid modulation of gene expression during Arabidopsis photomorphogenesis.
abstract: Profiling of DNA and histone modifications has recently allowed the establishment of reference epigenomes from several model organisms. This identified a major chromatin state for active genes that contains monoubiquitinated H2B (H2Bub), a mark linked to transcription elongation. However, assessment of dynamic chromatin changes during the reprogramming of gene expression in response to extrinsic or developmental signals has been more difficult. Here we used the major developmental switch that Arabidopsis thaliana plants undergo upon their initial perception of light, known as photomorphogenesis, as a paradigm to assess spatial and temporal dynamics of monoubiquitinated H2B (H2Bub) and its impact on transcriptional responses. The process involves rapid and extensive transcriptional reprogramming and represents a developmental window well suited to studying cell division-independent chromatin changes. Genome-wide H2Bub distribution was determined together with transcriptome profiles at three time points during early photomorphogenesis. This revealed de novo marking of 177 genes upon the first hour of illumination, illustrating the dynamic nature of H2Bub enrichment in a genomic context. Gene upregulation was associated with H2Bub enrichment, while H2Bub levels generally remained stable during gene downregulation. We further report that H2Bub influences the modulation of gene expression, as both gene up- and downregulation were globally weaker in hub1 mutant plants that lack H2Bub. H2Bub-dependent regulation notably impacted genes with fast and transient light induction, and several circadian clock components whose mRNA levels are tightly regulated by sharp oscillations. Based on these findings, we propose that H2B monoubiquitination is part of a transcription-coupled, chromatin-based mechanism to rapidly modulate gene expression.
authors: Ecole Normale Supérieure, Institut de Biologie de l'ENS, IBENS, Paris, France.
authors: Clara
authors: C
authors: Bourbousse
authors: Ikhlak
authors: I
authors: Ahmed
authors: François
authors: F
authors: Roudier
authors: Gérald
authors: G
authors: Zabulon
authors: Eddy
authors: E
authors: Blondet
authors: Sandrine
authors: S
authors: Balzergue
authors: Vincent
authors: V
authors: Colot
authors: Chris
authors: C
authors: Bowler
authors: Fredy
authors: F
authors: Barneche
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism; D006657:Histones / Q000235:genetics / Q000378:metabolism; D008027:Light; D009024:Morphogenesis / Q000235:genetics / Q000502:physiology; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D015533:Transcriptional Activation / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000502:physiology; D054875:Ubiquitination / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; D006657:Histones; D011495:Histone-Lysine N-Methyltransferase; C521255:HUB1 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases