title: Transient reduction and activation of circulating dendritic cells in patients with acute myocardial infarction.
abstract: BACKGROUND
  Dendritic cells (DCs) are highly potent professional antigen-presenting cells that play a central role in initiating the primary immune response. Accumulating evidence suggests that immune-mediated inflammation plays an important role in the pathophysiology of AMI, but the mechanism that triggers such immune responses is unknown.
  
  
  METHODS
  Using multi-color flow-cytometry, we determined the numbers of circulating myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) in patients with AMI (n = 26) or stable angina pectoris (SAP) (n = 19), and in age-matched control subjects (n = 19). The DC activation markers CD40 and CD83 were also measured.
  
  
  RESULTS
  On admission, circulating mDC and pDC counts were significantly lower in AMI patients compared to control subjects and SAP patients (mDC, P < 0.01; pDC, P < 0.05). The activation markers of mDCs in AMI patients were significantly higher and returned to the levels of control subjects or SAP patients 3 days after AMI (mDC, P < 0.05; pDC, P < 0.05). Reductions of circulating mDC and pDC numbers were restored 7 days after the onset of AMI. Furthermore, we found that the recovery of the circulating DC numbers 14 days after AMI was correlated with the alterations of creatine kinase-MB (CK-MB) (mDC, r = 0.48, P < 0.05; pDC, r=0.52, P < 0.01) and brain natriuretic peptide (BNP) (mDC, r = 0.53, P < 0.01; pDC, r = 0.51, P < 0.01).
  
  
  CONCLUSIONS
  Our findings suggest that the transient reduction and activation of circulating DCs may play important roles in the pathophysiology of myocardial injury after AMI.
authors: Division of Cardiovascular Medicine, Department of Internal Medicine, Kurume, Japan.
authors: Daisuke
authors: D
authors: Fukui
authors: Hideo
authors: H
authors: Yasukawa
authors: Yusuke
authors: Y
authors: Sugi
authors: Toyoharu
authors: T
authors: Oba
authors: Takanobu
authors: T
authors: Nagata
authors: Sachiko
authors: S
authors: Kyogoku
authors: Nobuyoshi
authors: N
authors: Futamata
authors: Toshiro
authors: T
authors: Yokoyama
authors: Shinji
authors: S
authors: Yokoyama
authors: Hisashi
authors: H
authors: Kai
authors: Takafumi
authors: T
authors: Ueno
authors: Masayoshi
authors: M
authors: Kage
authors: Tsutomu
authors: T
authors: Imaizumi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count / Q000379:methods; D003713:Dendritic Cells / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis; D013997:Time Factors