title: Time-dependent O2 consumption patterns determined optimal time ranges for selecting viable human embryos.
abstract: OBJECTIVE
  To evaluate correlations between metabolic activity and implantation potential of transferred embryos in a study based on oxygen (O(2)) consumption (OC) measurements, because O(2) uptake is directly related to the capacity of an embryo to produce energy via adenosine triphosphate.
  
  
  METHODS
  Retrospective cohort study.
  
  
  METHODS
  Infertility institute.
  
  
  METHODS
  Five hundred seventy-five injected oocytes in 56 first oocyte donation cycles with embryo transfer on day 3.
  
  
  METHODS
  None.
  
  
  METHODS
  We analyzed embryo destination viability and implantation depending on the embryo OC rate obtained from 47,741 measurements (up to 85 measurements per embryo, 2-3 measurements per hour). OC patterns were analyzed in relation to the time elapsed from sperm microinjection, to the final destination of the embryos (transferred, frozen, or discarded), to ongoing pregnancy, and by successful implantation.
  
  
  RESULTS
  OC was found to decrease during embryonic development. OC patterns from 52 hours onward showed the strongest correlation with implantation success. Regarding embryo destination, the same patterns were observed.
  
  
  CONCLUSIONS
  OC from individual embryos revealed significant differences, mainly close to the time of transfer, when OC pattern was associated with successful implantation. Therefore, measuring the OC pattern of human embryos culture up to 72 hours could be used to select the embryo with best developmental potential.
authors: Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain.
authors: Alberto
authors: A
authors: Tejera
authors: Javier
authors: J
authors: Herrero
authors: Thamara
authors: T
authors: Viloria
authors: Josep Lluis
authors: JL
authors: Romero
authors: Pilar
authors: P
authors: Gamiz
authors: Marcos
authors: M
authors: Meseguer
mesh_terms: D000022:Abortion, Spontaneous; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D015331:Cohort Studies; D010064:Embryo Implantation; D004624:Embryo Transfer / Q000379:methods*; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D006801:Humans; D018587:Oocyte Donation; D010062:Ovulation Induction / Q000379:methods; D010101:Oxygen Consumption / Q000502:physiology*; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D013997:Time Factors; D055815:Young Adult
chemical_list: D000255:Adenosine Triphosphate