title: Genomic and serological detection of bat coronavirus from bats in the Philippines.
abstract: Bat coronavirus (BtCoV) is assumed to be a progenitor of severe acute respiratory syndrome (SARS)-related coronaviruses. To explore the distribution of BtCoVs in the Philippines, we collected 179 bats and detected viral RNA from intestinal or fecal samples by RT-PCR. The overall prevalence of BtCoVs among bats was 29.6 %. Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene suggested that one of the detected BtCoVs was a novel alphacoronavirus, while the others belonged to the genus Betacoronavirus. Western blotting revealed that 66.5 % of bat sera had antibodies to BtCoV. These surveys suggested the endemic presence of BtCoVs in the Philippines.
authors: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors: Shumpei
authors: S
authors: Tsuda
authors: Shumpei
authors: S
authors: Watanabe
authors: Joseph S
authors: JS
authors: Masangkay
authors: Tetsuya
authors: T
authors: Mizutani
authors: Phillip
authors: P
authors: Alviola
authors: Naoya
authors: N
authors: Ueda
authors: Koichiro
authors: K
authors: Iha
authors: Satoshi
authors: S
authors: Taniguchi
authors: Hikaru
authors: H
authors: Fujii
authors: Kentaro
authors: K
authors: Kato
authors: Taisuke
authors: T
authors: Horimoto
authors: Shigeru
authors: S
authors: Kyuwa
authors: Yasuhiro
authors: Y
authors: Yoshikawa
authors: Hiroomi
authors: H
authors: Akashi
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D002685:Chiroptera; D017934:Coronavirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D018352:Coronavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D000086462:Coronavirus Nucleocapsid Proteins; D018076:DNA, Complementary / Q000737:chemistry; D005243:Feces / Q000821:virology; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D007422:Intestines / Q000821:virology; D008969:Molecular Sequence Data; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism; D010679:Philippines / Q000453:epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity
chemical_list: D000914:Antibodies, Viral; D000086462:Coronavirus Nucleocapsid Proteins; D018076:DNA, Complementary; D019590:Nucleocapsid Proteins; D012367:RNA, Viral