title: Application of a sensitive and specific LC-MS/MS method for determination of chinensinaphthol methyl ether in rat plasma for a bioavailability study.
abstract: Chinensinaphthol methyl ether (CME) is a potential pharmacologically active ingredient isolated from the dried plants of Justicia procumbens L. (Acanthaceae). A sensitive and specific LC-MS/MS method was developed and validated for the analysis of CME in rat plasma using buspirone as the internal standard (IS). The analyte was extracted with ethyl acetate and chromatographed on a reverse-phase Agilent Zorbax-C18 110 Å column (50 mm × 2.1mm, 3.5 μm). Elution was achieved with a gradient mobile phase consisting of water and acetonitrile both containing 0.1% formic acid at a flow rate of 0.40 mL/min. The analytes were monitored by tandem-mass spectrometry with positive electrospray ionization. The precursor/product transitions (m/z) in the positive ion mode were 394.5→346.0 and 386.1→122.0 for CME and IS, respectively. The assay was shown to be linear over the range of 0.50-500 ng/mL, with a lower limit of quantification of 0.50 ng/mL. The method was shown to be reproducible and reliable with the inter- and intra-day accuracy and precision were within ±15%. The assay has been successfully used for pharmacokinetic evaluation of CME after intravenous and oral administration of 1.80 mg/kg CME in rats. The oral absolute bioavailability (F) of CME was estimated to be 3.2±0.2% with an elimination half-life (t½) value of 2.4±0.8h, suggesting its poor absorption and/or strong metabolism in vivo.
authors: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
authors: Shujun
authors: S
authors: Zhou
authors: Feng
authors: F
authors: Qiu
authors: Zhanqi
authors: Z
authors: Tong
authors: Shihai
authors: S
authors: Yang
authors: Meihua
authors: M
authors: Yang
mesh_terms: D027901:Acanthaceae / Q000737:chemistry; D000818:Animals; D002065:Buspirone; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D016018:Least-Squares Analysis; D008297:Male; D009284:Naphthols / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009284:Naphthols; D010936:Plant Extracts; D002065:Buspirone