title: Evaluation of oxidative stress in mice subjected to aerobic exercise.
abstract: OBJECTIVE
  To evaluate the influence of aerobic exercise on oxidative stress in mice.
  
  
  METHODS
  The study included twenty female mice Mus musculus-Swiss divided into two groups: sedentary control (GA) and exercise (GB), each containing ten animals. All animals underwent an adaptation period of seven days isolated in individual boxes. After this period, the animals in the exercise group (GB) were trained in angled running wheel with circumference of 25 cm assembled on an articulated axle during five minutes for three consecutive days. On the fourth day, they underwent an exercise program of one session lasting 45 minutes. The evaluation of oxidative stress was performed by determining the levels of malondialhyde derived of lipid peroxidation by the TBA method. The samples were read in a spectrophotometer at 535 nm.
  
  
  RESULTS
  No significant difference was observed in the intergroup comparison of MDA levels in the tissues evaluated. A significant difference was observed in the intragroup comparison of MDA levels in the control group (p = 0.0201).The Tukeys' post hoc test indicated significantly lower values of MDA in the smooth muscle in relation to plasma. In the analysis of variance in the exercise group, a significant difference between tissues (p = 0.0009), with significantly lower values in the smooth muscle in relation to plasma (p<0.001) and higher in striated muscle in relation to smooth muscle (p<0.05) was observed.
  
  
  CONCLUSIONS
  There was no change in the analysis of oxidative stress in mice which were undergone a single session of aerobic exercise.
authors: UFMS, Campo Grande, MS, Brazil. monicacruvinel@hotmail.com
authors: Mônica Cruvinel de
authors: MC
authors: Lima
authors: Guido
authors: G
authors: Marks
authors: Iandara Schettert
authors: IS
authors: Silva
authors: Baldomero Antonio Kato da
authors: BA
authors: Silva
authors: Lourdes Zélia Zanoni
authors: LZ
authors: Cônsolo
authors: Gabriel Bogalho
authors: GB
authors: Nogueira
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D005260:Female; D015227:Lipid Peroxidation / Q000502:physiology; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D018482:Muscle, Skeletal / Q000737:chemistry; D018384:Oxidative Stress / Q000502:physiology*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology; D013858:Thiobarbiturates; D013997:Time Factors
chemical_list: D015415:Biomarkers; D013858:Thiobarbiturates; D008315:Malondialdehyde; C029684:thiobarbituric acid