title: Echinococcus granulosus 14-3-3 protein: a potential vaccine candidate against challenge with Echinococcus granulosus in mice.
abstract: OBJECTIVE
  To investigate the protective immunity against Echinococcus granulosus in mice immunized with rEg14-3-3.
  
  
  METHODS
  ICR mice were subcutaneously immunized three times with rEg14-3-3, followed by the challenge with Echinococcus granulosus protoscoleces intraperitoneally and then sacrificed after six months of post-challenge to detect the proliferation of splenocytes by MTT assay, and to measure the secretion of IL-2, IL-4, IL-10, and IFN-γ by ELISA. The rate of reduced hydatid cyst and the levels of IgE, IgG and IgG subclasses in sera were examined.
  
  
  RESULTS
  Mice vaccinated with rEg14-3-3 and challenged with protoscoleces revealed significant protective immunity of 84.47%. ELISA analysis indicated that the immunized mice generated specific high levels of IgG and the prevailing isotypes of IgG were IgG1 and IgG2a. Splenocytes from mice immunized with rEg14-3-3 showed a significant proliferation response. The secretion of IFN-γ and IL-2 increased significantly in the vaccinated mice whereas there was no significant difference in IL-4 and IL-10 levels between vaccinated and control mice.
  
  
  CONCLUSIONS
  The results indicate that the rEg14-3-3 vaccine could induce a high level of protective immunity as a promising vaccine candidate to prevent cystic echinococcosis.
authors: Center of Scientific Technology of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China.
authors: Zong Ji
authors: ZJ
authors: Li
authors: Ya Na
authors: YN
authors: Wang
authors: Qi
authors: Q
authors: Wang
authors: Wei
authors: W
authors: Zhao
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004443:Echinococcosis / Q000517:prevention & control*; D048209:Echinococcus granulosus / Q000235:genetics / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000502:physiology; D051379:Mice; D013154:Spleen / Q000166:cytology; D014612:Vaccines / Q000276:immunology*
chemical_list: D048948:14-3-3 Proteins; D016207:Cytokines; D014612:Vaccines