title: A central coupler for recombination initiation linking chromosome architecture to S phase checkpoint.
abstract: Higher-order chromosome structure is assumed to control various DNA-templated reactions in eukaryotes. Meiotic chromosomes implement developed structures called "axes" and "loops"; both are suggested to tether each other, activating Spo11 to catalyze meiotic DNA double-strand breaks (DSBs) at recombination hotspots. We found that the Schizosaccharomyces pombe Spo11 homolog Rec12 and its partners form two distinct subcomplexes, DSBC (Rec6-Rec12-Rec14) and SFT (Rec7-Rec15-Rec24). Mde2, whose expression is strictly regulated by the replication checkpoint, interacts with Rec15 to stabilize the SFT subcomplex and further binds Rec14 in DSBC. Rec10 provides a docking platform for SFT binding to axes and can partially interact with DSB sites located in loops depending upon Mde2, which is indicative of the formation of multiprotein-based tethered axis-loop complex. These data lead us to propose a mechanism by which Mde2 functions as a recombination initiation mediator to tether axes and loops, in liaison with the meiotic replication checkpoint.
authors: Department of Life Sciences, The University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan.
authors: Tomoichiro
authors: T
authors: Miyoshi
authors: Masaru
authors: M
authors: Ito
authors: Kazuto
authors: K
authors: Kugou
authors: Shintaro
authors: S
authors: Yamada
authors: Masaki
authors: M
authors: Furuichi
authors: Arisa
authors: A
authors: Oda
authors: Takatomi
authors: T
authors: Yamada
authors: Kouji
authors: K
authors: Hirota
authors: Hisao
authors: H
authors: Masai
authors: Kunihiro
authors: K
authors: Ohta
mesh_terms: D002875:Chromosomes / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D008540:Meiosis / Q000235:genetics; D011995:Recombination, Genetic; D016196:S Phase; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D004706:Endodeoxyribonucleases; D051858:Forkhead Transcription Factors; D029702:Schizosaccharomyces pombe Proteins; D000099257:Meiotic Recombination Protein SPO11; C509497:mde2 protein, S. pombe