title: Restoration of hearing in the VGLUT3 knockout mouse using virally mediated gene therapy.
abstract: Mice lacking the vesicular glutamate transporter-3 (VGLUT3) are congenitally deaf due to loss of glutamate release at the inner hair cell afferent synapse. Cochlear delivery of VGLUT3 using adeno-associated virus type 1 (AAV1) leads to transgene expression in only inner hair cells (IHCs), despite broader viral uptake. Within 2 weeks of AAV1-VGLUT3 delivery, auditory brainstem response (ABR) thresholds normalize, along with partial rescue of the startle response. Lastly, we demonstrate partial reversal of the morphologic changes seen within the afferent IHC ribbon synapse. These findings represent a successful restoration of hearing by gene replacement in mice, which is a significant advance toward gene therapy of human deafness.
authors: Department of Otolaryngology, Head and Neck Surgery, University of California San Francisco, San Francisco, CA 94143, USA.
authors: Omar
authors: O
authors: Akil
authors: Rebecca P
authors: RP
authors: Seal
authors: Kevin
authors: K
authors: Burke
authors: Chuansong
authors: C
authors: Wang
authors: Aurash
authors: A
authors: Alemi
authors: Matthew
authors: M
authors: During
authors: Robert H
authors: RH
authors: Edwards
authors: Lawrence R
authors: LR
authors: Lustig
mesh_terms: D026922:Amino Acid Transport Systems, Acidic / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D003638:Deafness / Q000235:genetics* / Q000378:metabolism / Q000628:therapy*; D000229:Dependovirus / Q000235:genetics; D016057:Evoked Potentials, Auditory, Brain Stem / Q000235:genetics*; D015316:Genetic Therapy / Q000379:methods*; D018698:Glutamic Acid / Q000378:metabolism; D006199:Hair Cells, Auditory, Inner / Q000378:metabolism; D006320:Hearing Tests; D051379:Mice; D018345:Mice, Knockout; D013216:Reflex, Startle / Q000235:genetics*; D013569:Synapses / Q000378:metabolism; D009435:Synaptic Transmission / Q000235:genetics
chemical_list: D026922:Amino Acid Transport Systems, Acidic; D018698:Glutamic Acid; C504941:Slc17a8 protein, mouse