title: Probing tubulin-blocked state of VDAC by varying membrane surface charge.
abstract: Reversible blockage of the voltage-dependent anion channel (VDAC) of the mitochondrial outer membrane by dimeric tubulin is being recognized as a potent regulator of mitochondrial respiration. The tubulin-blocked state of VDAC is impermeant for ATP but only partially closed for small ions. This residual conductance allows studying the nature of the tubulin-blocked state in single-channel reconstitution experiments. Here we probe this state by changing lipid bilayer charge from positive to neutral to negative. We find that voltage sensitivity of the tubulin-VDAC blockage practically does not depend on the lipid charge and salt concentration with the effective gating charge staying within the range of 10-14 elementary charges. At physiologically relevant low salt concentrations, the conductance of the tubulin-blocked state is decreased by positive and increased by negative charge of the lipids, whereas the conductance of the open channel is much less sensitive to this parameter. Such a behavior supports the model in which tubulin's negatively charged tail enters the VDAC pore, inverting its anionic selectivity to cationic and increasing proximity of ion pathways to the nearest lipid charges as compared with the open state of the channel.
authors: Program in Physical Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
authors: Philip A
authors: PA
authors: Gurnev
authors: Maria
authors: M
authors: Queralt-Martin
authors: Vicente M
authors: VM
authors: Aguilella
authors: Tatiana K
authors: TK
authors: Rostovtseva
authors: Sergey M
authors: SM
authors: Bezrukov
mesh_terms: D002463:Cell Membrane Permeability; D015640:Ion Channel Gating; D008051:Lipid Bilayers / Q000737:chemistry*; D008560:Membrane Fluidity; D008564:Membrane Potentials; D016062:Porosity; D050994:Voltage-Dependent Anion Channels / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D008051:Lipid Bilayers; D050994:Voltage-Dependent Anion Channels