title: Anorexic effect of peripheral cholecystokinin (CCK) varies with age and body composition (short communication).
abstract: Obesity of middle-aged mammals is followed at old age by anorexia and cachexia leading to sarcopenia. Complex age- and body composition-related alterations in the regulation of energy homeostasis may be assumed in the background. We aimed to test the possible contribution of age- and body composition-related changes of satiety responses to catabolic brain-gut-axis peptide cholecystokinin (CCK) to these alterations in energy balance during aging. Male Wistar rats (6-8 animals/group) aged 2 months (juvenile), 3 months (young adult), 6 or 12 months (early or late middle-aged), and 24 months (old) were injected intraperitoneally with 5 μg CCK-8 prior to re-feeding after 48-h food-deprivation. CCK suppressed re-feeding in young adult (26.8%), early middle-aged (35.5%), and old (31.4%) animals, but not in juvenile or late middle-aged rats (one-way ANOVA). CCK-resistance of 12 months old rats was prevented by life-long calorie-restriction: CCK suppressed their re-feeding by 46.8%. Conversely, in highfat diet-induced obese 6 months old rats CCK failed to suppress re-feeding. In conclusion, age-related changes in satiety responsiveness to CCK may contribute to the age-related obesity of middle-aged as well as to the anorexia of old animals. CCK-responsiveness is also influenced by body composition: calorie-restriction prevents the resistance to CCK, pre-existing obesity enhances it.
authors: University of Pécs, Department of Pathophysiology and Gerontology, Pécs, Hungary. marta.balasko@aok.pte.hu
authors: Márta
authors: M
authors: Balaskó
authors: Sz
authors: S
authors: Soós
authors: A
authors: A
authors: Párniczky
authors: M
authors: M
authors: Koncsecskó-Gáspár
authors: M
authors: M
authors: Székely
authors: E
authors: E
authors: Pétervári
mesh_terms: D050154:Adiposity; D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000855:Anorexia / Q000139:chemically induced* / Q000378:metabolism / Q000523:psychology; D001823:Body Composition; D031204:Caloric Restriction; D059305:Diet, High-Fat / Q000009:adverse effects; D004734:Energy Metabolism; D005247:Feeding Behavior; D006706:Homeostasis; D007274:Injections, Intraperitoneal; D008297:Male; D009765:Obesity / Q000209:etiology / Q000378:metabolism / Q000523:psychology; D051381:Rats; D017208:Rats, Wistar; D012528:Satiety Response; D012844:Sincalide / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D012844:Sincalide