title: Dynamic micro- and macrovascular remodeling in coronary circulation of obese Ossabaw pigs with metabolic syndrome.
abstract: Previous studies from our laboratory showed that coronary arterioles from type 2 diabetic mice undergo inward hypertrophic remodeling and reduced stiffness. The aim of the current study was to determine if coronary resistance microvessels (CRMs) in Ossabaw swine with metabolic syndrome (MetS) undergo remodeling distinct from coronary conduit arteries. Male Ossabaw swine were fed normal (n = 7, Lean) or hypercaloric high-fat (n = 7, MetS) diets for 6 mo, and then CRMs were isolated and mounted on a pressure myograph. CRMs isolated from MetS swine exhibited decreased luminal diameters (126 ± 5 and 105 ± 9 μm in Lean and MetS, respectively, P < 0.05) with thicker walls (18 ± 3 and 31 ± 3 μm in Lean and MetS, respectively, P < 0.05), which doubled the wall-to-lumen ratio (14 ± 2 and 30 ± 2 in Lean and MetS, respectively, P < 0.01). Incremental modulus of elasticity (IME) and beta stiffness index (BSI) were reduced in CRMs isolated from MetS pigs (IME: 3.6 × 10(6) ± 0.7 × 10(6) and 1.1 × 10(6) ± 0.2 × 10(6) dyn/cm(2) in Lean and MetS, respectively, P < 0.001; BSI: 10.3 ± 0.4 and 7.3 ± 1.8 in Lean and MetS, respectively, P < 0.001). BSI in the left anterior descending coronary artery was augmented in pigs with MetS. Structural changes were associated with capillary rarefaction, decreased hyperemic-to-basal coronary flow velocity ratio, and augmented myogenic tone. MetS CRMs showed a reduced collagen-to-elastin ratio, while immunostaining for the receptor for advanced glycation end products was selectively increased in the left anterior descending coronary artery. These data suggest that MetS causes hypertrophic inward remodeling of CRMs and capillary rarefaction, which contribute to decreased coronary flow and myocardial ischemia. Moreover, our data demonstrate novel differential remodeling between coronary micro- and macrovessels in a clinically relevant model of MetS.
authors: Center for Cardiovascular and Pulmonary Research, The Heart Center, The Research Institute at Nationwide Children's Hospital, 700 Children’s Drive, Columbus, OH 43205, USA. aaron.trask@nationwidechildrens.org
authors: Aaron J
authors: AJ
authors: Trask
authors: Paige S
authors: PS
authors: Katz
authors: Amy P
authors: AP
authors: Kelly
authors: Maarten L
authors: ML
authors: Galantowicz
authors: Mary J
authors: MJ
authors: Cismowski
authors: T Aaron
authors: TA
authors: West
authors: Zachary P
authors: ZP
authors: Neeb
authors: Zachary C
authors: ZC
authors: Berwick
authors: Adam G
authors: AG
authors: Goodwill
authors: Mouhamad
authors: M
authors: Alloosh
authors: Johnathan D
authors: JD
authors: Tune
authors: Michael
authors: M
authors: Sturek
authors: Pamela A
authors: PA
authors: Lucchesi
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D003094:Collagen / Q000378:metabolism; D003326:Coronary Circulation / Q000502:physiology*; D003331:Coronary Vessels / Q000378:metabolism / Q000503:physiopathology*; D004549:Elastin / Q000378:metabolism; D008297:Male; D024821:Metabolic Syndrome / Q000378:metabolism / Q000503:physiopathology*; D055806:Microvessels / Q000378:metabolism / Q000503:physiopathology*; D009765:Obesity / Q000378:metabolism / Q000503:physiopathology*; D013552:Swine
chemical_list: D003094:Collagen; D004549:Elastin