title: In vitro biocompatibility tests of glass ionomer cements impregnated with collagen or bioactive glass to fibroblasts.
abstract: OBJECTIVE
  To evaluate the biocompatibility of glass ionomer cement (GIC) impregnated with collagen or bioactive glass to BHK-21 fibroblasts in vitro. Mineral Trioxide Aggregate was used as the standard for comparison. Human maxillary central incisors (n = 70) were instrumented with a rotary NiTi system and filled. Following resection of the apical 3mm, root end cavities were prepared and restored with conventional GIC (group 1) or GIC with 0.01%, 0.1% or 1% collagen (groups 2, 3, 4 respectively) or, 10%, 30% or 50% bioactive glass (groups 5, 6, 7 respectively), or Mineral Trioxide Aggregate (group 8). The root slices were incubated in tissue culture plates with BHK-21 fibroblast cell line. Phase contrast and scanning electron microscopes were used to score cell quantity, morphology and cell attachment. The data were statistically analyzed by one way ANOVA with Post Hoc Tukey HSD test (p = 0.05).
  
  
  CONCLUSIONS
  Group 5 showed the highest scores which was significantly higher than all other groups (p < 0.05) except group 8, with which there was no significant difference (p > 0.05). Glass ionomer cement with 10% bioactive glass showed better adhesion and spreading of cells than glass ionomer cement with 0.01% collagen. The biocompatibility of collagen and bioactive glass was concentration dependent. The addition of bioactive glass improved the biocompatibility of glass ionomer cement to fibroblasts better than addition of collagen.
authors: Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha University, Chennai, India.
authors: C
authors: C
authors: Subbarao
authors: P
authors: P
authors: Neelakantan
authors: C V
authors: CV
authors: Subbarao
mesh_terms: D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D001047:Apicoectomy / Q000379:methods; D001672:Biocompatible Materials / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D018929:Cell Culture Techniques; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D006224:Cricetinae; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects*; D005898:Glass; D005899:Glass Ionomer Cements / Q000494:pharmacology*; D006801:Humans; D007180:Incisor / Q000473:pathology; D008422:Materials Testing; D008647:Mesocricetus; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D010087:Oxides / Q000494:pharmacology; D012184:Retrograde Obturation / Q000379:methods; D012387:Root Canal Filling Materials / Q000494:pharmacology; D017640:Silicates / Q000494:pharmacology
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D005899:Glass Ionomer Cements; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C425330:bioactive glass 45S5; C086631:mineral trioxide aggregate; D003094:Collagen