title: Structural similarities of fucoidans from brown algae Silvetia babingtonii and Fucus evanescens, determined by tandem MALDI-TOF mass spectrometry.
abstract: Rapid mass spectrometric investigation of oligosaccharides, obtained by autohydrolysis of fucoidans from brown algae Silvetia babingtonii and Fucus evanescens (Fucales, Phaeophyceae) has shown both similarities and differences in structural features/sulfation pattern of their fragments, obtained in the same conditions. Tandem MALDI-TOF MS of fucooligosaccharides with even DP (degree of polymerization) was close to that observed for fucoidan from F. evanescens. Slight differences in tandem mass spectra of fragments with odd DP indicated, probably, sulfation at C-3 (instead of C-2 in F. evanescens) of some (1→4)-linked α-L-Fucp residues and/or the presence of short blocks, built up of (1→3)-linked α-L-Fucp residues.
authors: GB Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia. sanastyuk@piboc.dvo.ru
authors: Stanislav D
authors: SD
authors: Anastyuk
authors: Natalia M
authors: NM
authors: Shevchenko
authors: Pavel S
authors: PS
authors: Dmitrenok
authors: Tatyana N
authors: TN
authors: Zvyagintseva
mesh_terms: D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D000459:Phaeophyceae / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; C007789:fucoidan