title: Identification of miRNA signatures during the differentiation of hESCs into retinal pigment epithelial cells.
abstract: Retinal pigment epithelium (RPE) cells can be obtained through in vitro differentiation of both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). We have previously identified 87 signature genes relevant to RPE cell differentiation and function through transcriptome analysis of both human ESC- and iPSC-derived RPE as well as normal fetal RPE. Here, we profile miRNA expression through small RNA-seq in human ESCs and their RPE derivatives. Much like conclusions drawn from our previous transcriptome analysis, we find that the overall miRNA landscape in RPE is distinct from ESCs and other differentiated somatic tissues. We also profile miRNA expression during intermediate stages of RPE differentiation and identified unique subsets of miRNAs that are gradually up- or down-regulated, suggesting that dynamic regulation of these miRNAs is associated with the RPE differentiation process. Indeed, the down-regulation of a subset of miRNAs during RPE differentiation is associated with up-regulation of RPE-specific genes, such as RPE65, which is exclusively expressed in RPE. We conclude that miRNA signatures can be used to classify different degrees of in vitro differentiation of RPE from human pluripotent stem cells. We suggest that RPE-specific miRNAs likely contribute to the functional maturation of RPE in vitro, similar to the regulation of RPE-specific mRNA expression.
authors: Translational Center for Stem Cell Research, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
authors: Ganlu
authors: G
authors: Hu
authors: Kevin
authors: K
authors: Huang
authors: Juehua
authors: J
authors: Yu
authors: Sailesh
authors: S
authors: Gopalakrishna-Pillai
authors: Jun
authors: J
authors: Kong
authors: He
authors: H
authors: Xu
authors: Zhenshan
authors: Z
authors: Liu
authors: Kunshan
authors: K
authors: Zhang
authors: Jun
authors: J
authors: Xu
authors: Yuping
authors: Y
authors: Luo
authors: Siguang
authors: S
authors: Li
authors: Yi E
authors: YE
authors: Sun
authors: Linda E
authors: LE
authors: Iverson
authors: Zhigang
authors: Z
authors: Xue
authors: Guoping
authors: G
authors: Fan
mesh_terms: D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D035683:MicroRNAs / Q000096:biosynthesis*; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000378:metabolism*; D059467:Transcriptome / Q000502:physiology*; D019745:cis-trans-Isomerases / Q000096:biosynthesis; D000099178:Retinoid Isomerohydrolase
chemical_list: D035683:MicroRNAs; D019745:cis-trans-Isomerases; D000099178:Retinoid Isomerohydrolase