title: Type II natural killer T cells use features of both innate-like and conventional T cells to recognize sulfatide self antigens.
abstract: Glycolipids presented by the major histocompatibility complex (MHC) class I homolog CD1d are recognized by natural killer T cells (NKT cells) characterized by either a semi-invariant T cell antigen receptor (TCR) repertoire (type I NKT cells or iNKT cells) or a relatively variable TCR repertoire (type II NKT cells). Here we describe the structure of a type II NKT cell TCR in complex with CD1d-lysosulfatide. Both TCR α-chains and TCR β-chains made contact with the CD1d molecule with a diagonal footprint, typical of MHC-TCR interactions, whereas the antigen was recognized exclusively with a single TCR chain, similar to the iNKT cell TCR. Type II NKT cell TCRs, therefore, recognize CD1d-sulfatide complexes by a distinct recognition mechanism characterized by the TCR-binding features of both iNKT cells and conventional peptide-reactive T cells.
authors: Division of Cell Biology, La Jolla Institute for Allergy & Immunology, La Jolla, California, USA.
authors: Enrico
authors: E
authors: Girardi
authors: Igor
authors: I
authors: Maricic
authors: Jing
authors: J
authors: Wang
authors: Thien-Thi
authors: TT
authors: Mac
authors: Pooja
authors: P
authors: Iyer
authors: Vipin
authors: V
authors: Kumar
authors: Dirk M
authors: DM
authors: Zajonc
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D055610:Antigens, CD1d / Q000737:chemistry / Q000276:immunology; D001324:Autoantigens / Q000276:immunology*; D003460:Crystallization; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D020836:Protein Structure, Quaternary; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry* / Q000276:immunology; D013433:Sulfoglycosphingolipids / Q000276:immunology; D020349:Surface Plasmon Resonance; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology*
chemical_list: D055610:Antigens, CD1d; D001324:Autoantigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013433:Sulfoglycosphingolipids