title: Effects of cryopreservation of Phaseolus vulgaris L. seeds on early stages of germination.
abstract: In this work, we studied the effects of cryopreservation on various parameters of early stages of germination of Phaseolus vulgaris seeds (0, 7 and 14 days). Percentages of germination, fresh mass of different plant parts, levels of chlorophyll pigments (a, b, total), malondialdehyde, other aldehydes, phenolics (cell wall-linked, free, and total) and protein were determined. No phenotypic changes were observed visually in seedlings recovered from cryopreserved seeds. However, several significant effects of seed liquid nitrogen exposure were recorded at the biochemical level. There was a significant negative effect of cryopreservation on shoot protein content, which decreased from 3.11 mg g(-1) fresh weight for non-cryopreserved controls to 0.44 mg g(-1) fresh shoot weight for cryopreserved seeds. On the other hand, cryopreservation significantly increased levels of other aldehydes than malondialdehyde in shoots at day 7, from 56.47 μmol g(-1) for non-cryopreserved controls to 253.19 μmol g(-1) fresh shoot weight for cryopreserved samples. Liquid nitrogen exposure significantly reduced phenolics contents (free, cell-wall linked, total) in roots at day 7 after onset of germination. In general, roots were more affected by cryostorage compared with other plant parts, while leaves were the least affected. The effects of seed cryopreservation seem to decline progressively along with seedling growth.
authors: Faculty of Agronomy, Universidad de Ciego de Ávila, Ciego de Ávila, 69450, Cuba.
authors: Inaudis
authors: I
authors: Cejas
authors: Karel
authors: K
authors: Vives
authors: Taletha
authors: T
authors: Laudat
authors: Justo
authors: J
authors: González-Olmedo
authors: Florent
authors: F
authors: Engelmann
authors: Marcos Edel
authors: ME
authors: Martínez-Montero
authors: José Carlos
authors: JC
authors: Lorenzo
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D018548:Cotyledon / Q000254:growth & development / Q000378:metabolism; D015925:Cryopreservation; D018525:Germination / Q000502:physiology*; D008315:Malondialdehyde / Q000378:metabolism; D027805:Phaseolus / Q000254:growth & development / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism; D010641:Phenotype; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors
chemical_list: D010636:Phenols; D002734:Chlorophyll; D008315:Malondialdehyde