title: TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity.
abstract: Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) are key RNA viral sensors for triggering antiviral immunity. The underlying mechanisms for RLRs to trigger antiviral immunity have yet to be explored. Here we report the identification of TAPE (TBK1-associated protein in endolysosomes) as a novel regulator of the RLR pathways. TAPE functionally and physically interacts with RIG-I, MDA5, and IPS-1 to activate the IFN-β promoter. TAPE knockdown impairs IFN-β activation induced by RLRs but not IPS-1. TAPE-deficient cells are defective in cytokine production upon RLR ligand stimulation. During RNA virus infection, TAPE knockdown or deficiency diminishes cytokine production and antiviral responses. Our data demonstrate a critical role for TAPE in linking RLRs to antiviral immunity.
authors: Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.
authors: Kuan-Ru
authors: KR
authors: Chen
authors: Chun-Hung
authors: CH
authors: Chang
authors: Ching-Yu
authors: CY
authors: Huang
authors: Chun-Yang
authors: CY
authors: Lin
authors: Wan-Ying
authors: WY
authors: Lin
authors: Yin-Chiu
authors: YC
authors: Lo
authors: Chia-Yu
authors: CY
authors: Yang
authors: En-Wei
authors: EW
authors: Hsing
authors: Lin-Fang
authors: LF
authors: Chen
authors: Shin-Ru
authors: SR
authors: Shih
authors: Ai-Li
authors: AL
authors: Shiau
authors: Huan-Yao
authors: HY
authors: Lei
authors: Tse-Hua
authors: TH
authors: Tan
authors: Pin
authors: P
authors: Ling
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D007107:Immune System; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry; D034622:RNA Interference; D011971:Receptors, Immunologic; D012097:Repressor Proteins / Q000737:chemistry* / Q000378:metabolism; D015398:Signal Transduction; D014709:Vero Cells
chemical_list: D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases; D004268:DNA-Binding Proteins; D017346:Protein Serine-Threonine Kinases; D011971:Receptors, Immunologic; D012097:Repressor Proteins; C512283:CC2D1A protein, human; C522738:Cc2d1a protein, mouse; C498588:Tbk1 protein, mouse; C403191:TBK1 protein, human; C452820:RIGI protein, human; C503356:Ddx58 protein, mouse