title: Restricted expression of Neuroglobin in the mouse retina and co-localization with Melanopsin and Tyrosine Hydroxylase.
abstract: Neuroglobin (Ngb), a neuronal specific oxygen binding heme-globin, reported to be expressed at high levels in most layers of the murine retina. Ngb's function is presently unknown, but based on its high expression level and oxygen binding capabilities Ngb was proposed to function as an oxygen reservoir facilitating oxygen metabolism in highly active neurons or to function as a neuroprotectant. In the present study, we re-examined the expression pattern of Ngb in the retina using a highly validated antibody. Furthermore, intactness of retino-hypothalamic projections and the retinal expression level of Melanopsin and Tyrosine Hydroxylase were investigated in Ngb-null mice. Ngb-immunoreactivity was found in a few neurons of the ganglion cell and inner nuclear layers co-expressing Melanopsin and Tyrosine Hydroxylase, respectively. Ngb deficiency neither affected the level of Melanopsin and Tyrosine Hydroxylase proteins nor the intactness of PACAP-positive retinohypothalamic projections in the suprachiasmatic nucleus. Based on the present results, it seems unlikely that Ngb could have a major role in retinal oxygen homeostasis and neuronal survival under normal conditions. The present study suggests that a number of previously published reports have relied on antibodies with dubious specificity.
authors: Department of Clinical Biochemistry, Bispebjerg Hospital, University of Copenhagen, Copenhagen, Denmark. c.hundahl@gmail.com
authors: C A
authors: CA
authors: Hundahl
authors: J
authors: J
authors: Fahrenkrug
authors: H
authors: H
authors: Luuk
authors: A
authors: A
authors: Hay-Schmidt
authors: J
authors: J
authors: Hannibal
mesh_terms: D000818:Animals; D005914:Globins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000078787:Neuroglobin; D010100:Oxygen / Q000378:metabolism; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D017299:Rod Opsins / Q000378:metabolism*; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D000099210:Melanopsin
chemical_list: D005914:Globins; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D010100:Oxygen; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D017299:Rod Opsins; D014446:Tyrosine 3-Monooxygenase; D000099210:Melanopsin