title: Copy number variations (CNVs) in human pluripotent cell-derived neuroprogenitors.
abstract: Results from the analysis of copy number variations (CNVs) in human pluripotent cell-derived neuroprogenitor cell lines (hiPSC and hESC-derived NPC) are presented. Two different types of CNVs were detected: a) CNVs inherited from the original source of pluripotent cells (hESC and hiPSC) and b) CNVs detected either in the original source of pluripotent cells or in the derived NPC cell lines but not in both at the same time. Our data suggest that submicroscopic chromosomal changes happened during culture and manipulation of cells and those differentiation procedures could result in gains and losses of genomic regions in pluripotent cell-derived neuroprogenitors. Overall, the results indicate that even chromosomally stable stem cell lines would need to be analyzed in detail by high resolution methodologies before their clinical use.
authors: CnPQ Fellowship, Brazil.
authors: Nestor L Lopez
authors: NL
authors: Corrales
authors: Kristin
authors: K
authors: Mrasek
authors: Martin
authors: M
authors: Voigt
authors: Thomas
authors: T
authors: Liehr
authors: Nadezda
authors: N
authors: Kosyakova
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations; D018628:Gene Dosage; D042822:Genomic Instability; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D007621:Karyotyping; D008957:Models, Genetic; D009474:Neurons / Q000166:cytology*; D012559:Schizophrenia / Q000235:genetics