title: [Effect of opioid peptides on oxygen-dependent microbicidity of peripheral blood neutrophils].
abstract: Investigation ofopioid peptide effect on the production of reactive oxygen species by neutrophils in non-fractionated leukocyte suspension and in purified fraction of peripheral blood neutrophils is disclosed in this work. It was determined that selective delta- and micro-agonists of peptide origin stimulated the spontaneous and suppressed 15 mkg/ml zymosan-induced LDCL (luminol-dependent chemiluminescence) reaction of neutrophils in leukocyte suspension. beta-endorphin was found to render less marked suppressive action on 15 mkg/ml zymosan-induced LDCL, and delta2-agonist deltorphin 2 promoted 15 mkg/ml zymosan-induced LDCL only toward the 25 minutes of the experiment. beta-endorphin and selective d- and m- agonists did not affect the spontaneous and suppressed 15 mkg/ml and 150 mkg/ml zymosan-induced neutrophil LDCL. Therefore, opioid peptides play essential role in the process of direct and indirect regulation of oxygen-dependent system of neutrophil granulocyte bactericidal activity.
authors: S V
authors: SV
authors: Geĭn
authors: K G
authors: KG
authors: Gorshkova
mesh_terms: D000328:Adult; D002450:Cell Communication / Q000502:physiology; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / Q000494:pharmacology; D016308:Enkephalin, Leucine-2-Alanine / Q000494:pharmacology; D005260:Female; D006801:Humans; D049408:Luminescent Agents / Q000494:pharmacology; D008165:Luminol / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000008:administration & dosage; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D009842:Oligopeptides / Q000008:administration & dosage; D018847:Opioid Peptides / Q000097:blood; D017382:Reactive Oxygen Species / Q000097:blood; D011957:Receptors, Opioid / Q000819:agonists* / Q000378:metabolism; D015054:Zymosan / Q000008:administration & dosage*; D001615:beta-Endorphin / Q000008:administration & dosage
chemical_list: D049408:Luminescent Agents; D018377:Neurotransmitter Agents; D009842:Oligopeptides; D018847:Opioid Peptides; D017382:Reactive Oxygen Species; D011957:Receptors, Opioid; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; C060209:deltorphin II, Ala(2)-; D008165:Luminol; D001615:beta-Endorphin; D016308:Enkephalin, Leucine-2-Alanine; D015054:Zymosan