title: Screening α-glucosidase inhibitors from mulberry extracts via DOSY and relaxation-edited NNR.
abstract: Inhibition of the α-glucosidase activity is a therapeutic approach for diabetes. In this study, an effective strategy for screening α-glucosidase inhibitors based on Nuclear magnetic resonance (NMR) techniques was developed to screen and identify α-glucosidase inhibitors from Mulberry leaf extract. As a result, deoxynojirimycin, as a potential α-glucosidase inhibitor, was found. The study suggested that our strategy was a powerful tool for screening and identification of α-glucosidase inhibitors in complex samples. Furthermore the interaction between α-glucosidase and its inhibitor was studied by NMR.
authors: Beijing National Laboratory for Molecular Sciences (BNLMS), Centre for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors: Qian
authors: Q
authors: Shang
authors: Jun-Feng
authors: JF
authors: Xiang
authors: Ya-Lin
authors: YL
authors: Tang
mesh_terms: D017485:1-Deoxynojirimycin / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology*; D005944:Glucosamine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D008024:Ligands; D010936:Plant Extracts; C501633:deoxynojirimycine; D017485:1-Deoxynojirimycin; D000520:alpha-Glucosidases; D005944:Glucosamine