title: Sequencing of the smallest Apicomplexan genome from the human pathogen Babesia microti.
abstract: We have sequenced the genome of the emerging human pathogen Babesia microti and compared it with that of other protozoa. B. microti has the smallest nuclear genome among all Apicomplexan parasites sequenced to date with three chromosomes encoding ∼3500 polypeptides, several of which are species specific. Genome-wide phylogenetic analyses indicate that B. microti is significantly distant from all species of Babesidae and Theileridae and defines a new clade in the phylum Apicomplexa. Furthermore, unlike all other Apicomplexa, its mitochondrial genome is circular. Genome-scale reconstruction of functional networks revealed that B. microti has the minimal metabolic requirement for intraerythrocytic protozoan parasitism. B. microti multigene families differ from those of other protozoa in both the copy number and organization. Two lateral transfer events with significant metabolic implications occurred during the evolution of this parasite. The genomic sequencing of B. microti identified several targets suitable for the development of diagnostic assays and novel therapies for human babesiosis.
authors: Laboratoire de Biologie Cellulaire et Moléculaire (LBCM-EA4558), UFR Pharmacie, Université Montpellier 1, 15, av. Charles Flahault, 34093 Montpellier cedex 5, France. emmanuel.cornillot@univ-montp1.fr
authors: Emmanuel
authors: E
authors: Cornillot
authors: Kamel
authors: K
authors: Hadj-Kaddour
authors: Amina
authors: A
authors: Dassouli
authors: Benjamin
authors: B
authors: Noel
authors: Vincent
authors: V
authors: Ranwez
authors: Benoît
authors: B
authors: Vacherie
authors: Yoann
authors: Y
authors: Augagneur
authors: Virginie
authors: V
authors: Brès
authors: Aurelie
authors: A
authors: Duclos
authors: Sylvie
authors: S
authors: Randazzo
authors: Bernard
authors: B
authors: Carcy
authors: Françoise
authors: F
authors: Debierre-Grockiego
authors: Stéphane
authors: S
authors: Delbecq
authors: Karina
authors: K
authors: Moubri-Ménage
authors: Hosam
authors: H
authors: Shams-Eldin
authors: Sahar
authors: S
authors: Usmani-Brown
authors: Frédéric
authors: F
authors: Bringaud
authors: Patrick
authors: P
authors: Wincker
authors: Christian P
authors: CP
authors: Vivarès
authors: Ralph T
authors: RT
authors: Schwarz
authors: Theo P
authors: TP
authors: Schetters
authors: Peter J
authors: PJ
authors: Krause
authors: André
authors: A
authors: Gorenflot
authors: Vincent
authors: V
authors: Berry
authors: Valérie
authors: V
authors: Barbe
authors: Choukri
authors: C
authors: Ben Mamoun
mesh_terms: D041001:Babesia microti / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D018503:Genome, Protozoan; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D017261:Glycosylphosphatidylinositols; D020543:Proteome