title: RAPD analysis of genetic  diversity and population structure of Elymus sibiricus (Poaceae) native  to the southeastern Qinghai-Tibet Plateau, China.
abstract: Genetic  diversity of Elymus sibiricus (Poaceae) was examined in eight  populations from the southeast Qinghai-Tibet Plateau. We detected 291 RAPD  polymorphic loci in 93 samples. The percentage of polymorphic bands (PPB)  was 79%. Genetic diversity (H(E)) was  0.264, effective number of alleles (N(E)) was  1.444, Shannon's information index (H(O)) was  0.398, and expected Bayesian heterozygosity (H(B)) was  0.371. At the population level, PPB = 51%, N(E) =  1.306, H(E) = 0.176, I = 0.263, and H(B) =  0.247. A high level of genetic differentiation was detected based on Nei's  genetic diversity analysis (G(ST) =  32.0%), Shannon's index analysis (33.7%), and the Bayesian method (θ(B) =  33.5%). The partitioning of molecular variance by AMOVA demonstrated  significant genetic differentiation within populations (60%) and among  populations (40%). The average number of individuals exchanged between populations  per generation (N(m)) was  1.06. The populations were found to share high levels of genetic identity. No  significant correlation was found between geographic distance and pairwise  genetic distance (r = 0.7539, P = 0.9996). Correlation analysis revealed  a significant correlation (r = 0.762) between RAPD H(E) found  in this study and ISSR H(E) values  from a previous study.
authors: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Ya'an, Sichuan, P.R. China.
authors: X
authors: X
authors: Ma
authors: S-Y
authors: SY
authors: Chen
authors: S-Q
authors: SQ
authors: Bai
authors: X-Q
authors: XQ
authors: Zhang
authors: D-X
authors: DX
authors: Li
authors: C-B
authors: CB
authors: Zhang
authors: J-J
authors: JJ
authors: Yan
mesh_terms: D000531:Altitude; D031749:Elymus / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D005843:Geography; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D018709:Statistics, Nonparametric; D018609:Tibet
chemical_list: D005819:Genetic Markers