title: Nanodiamond for intracellular imaging in the microorganisms in vivo.
abstract: Nanodiamond (ND) has great potential for bio labeling and drug delivery. In this work, the biocompatibility and bio labeling of ND are demonstrated via the interaction with cells and microorganisms, protists microorganisms Paramecium caudatum and Tetrahymena thermophile, in vitro and in vivo. We found the microorganism's living functions are not significantly affected by ND. The NDs were found entering the food vacuoles and later excreted by the microorganisms. The 5 nm ND was found more toxic compared to 100 nm ND, presumably due to the surface disordered carbons. Our results demonstrated nanodiamond can be used in bio imaging and matter delivery.
authors: Department of Physics, National Dong Hwa University, Hualien, Taiwan.
authors: Yu-Chung
authors: YC
authors: Lin
authors: Elena
authors: E
authors: Perevedentseva
authors: Lin-Wei
authors: LW
authors: Tsai
authors: Kuan-Ting
authors: KT
authors: Wu
authors: Chia-Liang
authors: CL
authors: Cheng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism*; D002460:Cell Line; D002923:Cilia / Q000378:metabolism; D042541:Intracellular Space / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D058612:Nanodiamonds; D048488:Paramecium caudatum / Q000166:cytology* / Q000378:metabolism; D010316:Particle Size; D016808:Tetrahymena thermophila / Q000166:cytology* / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D058612:Nanodiamonds