title: Folate-conjugated thermo-responsive micelles for tumor targeting.
abstract: Folate-conjugated and thermo-responsive poly((N-isopropylacrylamide)-co- acrylamide-co-(octadecyl acrylate)-co-(folate-(polyethylene glycol)-(acrylic acid))) (P(NIPA-co-AAm-co-ODA-co-FPA)) micelles with mean diameter of about 60 nm and lower critical solution temperature (LCST) of about 39°C were synthesized by free radical random copolymerization. Single-factor tests of acrylamide and octadecyl acrylate were carried out to modulate micelles' LCST and diameter, respectively. LCST, diameter, and morphology of micelles were determined by UV-vis spectrophotometer, laser particle size analyzer, and transmittance electron microscope (TEM), respectively. Fluorescein was then used as a model drug to investigate the drug loading content of micelles. Micelles with maximum amount of octadecyl acrylate (180 mg) were found to yield drug loading content of 10.48%. Near infrared dye No.10 was chosen as the tracer to monitor micelles in vivo. The targeting behaviors of micelles in folate receptor positive Bel-7402 tumor bearing nude mice were assessed by a self-constructed near infrared imaging system. Results showed satisfactory targeting capability of the thermo-responsive micelles toward Bel-7402 tumors, and targeting accumulation could last for more than 96 h, enabling P(NIPA-co-AAm-co-ODA-co-FPA) micelles to function as a diagnostic reagent as well as a targeted tumor therapy.
authors: Department of Biomedical Engineering, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors: Jian
authors: J
authors: Zhang
authors: Dawei
authors: D
authors: Deng
authors: Hongyan
authors: H
authors: Zhu
authors: Youngro
authors: Y
authors: Byun
authors: Victor C
authors: VC
authors: Yang
authors: Yueqing
authors: Y
authors: Gu
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000179:Acrylates / Q000737:chemistry; D000818:Animals; D002460:Cell Line; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000378:metabolism*; D016503:Drug Delivery Systems; D005260:Female; D058973:Folate Receptors, GPI-Anchored / Q000032:analysis / Q000378:metabolism*; D005492:Folic Acid / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008823:Micelles; D009369:Neoplasms / Q000175:diagnosis / Q000188:drug therapy* / Q000378:metabolism; D013696:Temperature
chemical_list: D000179:Acrylates; D003692:Delayed-Action Preparations; D058973:Folate Receptors, GPI-Anchored; D008823:Micelles; D020106:Acrylamide; D005492:Folic Acid