title: Expression patterns of chitinase produced from Paenibacillus chitinolyticus with different two culture media.
abstract: To investigate the expression patterns of chitinase isozymes on native-PAGE and SDS-PAGE gels Paenibacillus chitinolyticus MP-306 was cultured on culture media with and without chitin substrate. P. chitinolyticus MP-306 had a strong chitinolytic activity on colloidal chitin medium. Chitinase isozymes of MP-306 were expressed as six bands (CN1-CN6) on native-PAGE gels and thirteen bands (CS1-CS13) on SDS-PAGE gels after incubation in chitin medium. Three bands (CN1, CN2, and CN3) of chitinase isozymes of MP-306 on native-PAGE gels were expressed as nine bands (CS1, CS2, CS3, CS4, CS5, CS6, CS8, CS10, and CS13) of chitinase isozymes on SDS-PAGE gels. Three bands (CN4, CN5, and CN6) of chitinase isozymes of MP-306 were strongly inhibited by metal ions on native-PAGE and SDS-PAGE gels.
authors: Division of Applied Bioscience and Biotechnology, Institute of Environmently Friendly Agriculture, College of Agricultural and Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors: Yong-Su
authors: YS
authors: Song
authors: Dong-Jun
authors: DJ
authors: Seo
authors: Kil-Yong
authors: KY
authors: Kim
authors: Ro-Dong
authors: RD
authors: Park
authors: Woo-Jin
authors: WJ
authors: Jung
mesh_terms: D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007477:Ions / Q000494:pharmacology; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008670:Metals / Q000494:pharmacology; D008828:Microbiological Techniques / Q000379:methods; D056507:Paenibacillus / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D003470:Culture Media; D007477:Ions; D007527:Isoenzymes; D008670:Metals; D002686:Chitin; D002688:Chitinases