title: Extraction, isolation, and purification of analytes from samples of marine origin--a multivariate task.
abstract: The development of a multivariate study for a quantitative analysis of six different polybrominated diphenyl ethers (PBDEs) in tissue of Atlantic Salmo salar L. is reported. An extraction, isolation, and purification process based on an accelerated solvent extraction system was designed, investigated, and optimized by means of statistical experimental design and multivariate data analysis and regression. An accompanying gas chromatography-mass spectrometry analytical method was developed for the identification and quantification of the analytes, BDE 28, BDE 47, BDE 99, BDE 100, BDE 153, and BDE 154. These PBDEs have been used in commercial blends that were used as flame-retardants for a variety of materials, including electronic devices, synthetic polymers and textiles. The present study revealed that an extracting solvent mixture composed of hexane and CH₂Cl₂ (10:90) provided excellent recoveries of all of the six PBDEs studied herein. A somewhat lower polarity in the extracting solvent, hexane and CH₂Cl₂ (40:60) decreased the analyte %-recoveries, which still remain acceptable and satisfactory. The study demonstrates the necessity to perform an intimately investigation of the extraction and purification process in order to achieve quantitative isolation of the analytes from the specific matrix.
authors: National Institute of Nutrition and Seafood Research, Strandgaten 229, P.O. Box 2029 Nordnes, N-5817 Bergen, Norway. Lucia.Liguori@kj.uib.no
authors: Lucia
authors: L
authors: Liguori
authors: Hans-René
authors: HR
authors: Bjørsvik
mesh_terms: D000818:Animals; D005411:Flame Retardants / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D055768:Halogenated Diphenyl Ethers / Q000032:analysis* / Q000302:isolation & purification*; D020317:Salmo salar; D017747:Seafood / Q000032:analysis*; D012623:Seawater / Q000032:analysis; D052616:Solid Phase Extraction / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; D014874:Water Pollutants, Chemical