title: Improved β-carotene biosynthesis and gene transcription in Blakeslea trispora with arachidonic acid.
abstract: With 0.4 g l(-1) arachidonic acid (AA) added to the medium after 36 h fermentation, β-carotene production in mated cultures of Blakeslea trispora was 73 % higher than that of the control at 690 mg l(-1). With the treatment of AA, the transcriptional levels of genes hmgR, carRA and carB, that are involved in carotene biosynthesis, increased by 31, 22 and 39 %, respectively.
authors: State Key Laboratory of Chemical Resource Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People's Republic of China. xianmeihu@126.com
authors: Xianmei
authors: X
authors: Hu
authors: Jie
authors: J
authors: Sun
authors: Qipeng
authors: Q
authors: Yuan
mesh_terms: D016718:Arachidonic Acid / Q000494:pharmacology*; D003470:Culture Media; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000235:genetics; D053858:Metabolic Networks and Pathways; D009090:Mucorales / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D019207:beta Carotene / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D012333:RNA, Messenger; D019207:beta Carotene; D016718:Arachidonic Acid