title: Cadmium toxicity is alleviated by AtLCD and AtDCD in Escherichia coli.
abstract: OBJECTIVE
  Arabidopsis thaliana l- and d-cysteine desulfhydrases (AtLCD and AtDCD) are two important H(2) S-generating enzymes. This study determined the effects of H(2) S derived from AtLCD and AtDCD on cadmium (Cd) toxicity in Escherichia coli.
  
  
  RESULTS
  AtLCD and AtDCD were cloned into pET28a vectors and transformed into wild-type E. coli strain BL21(DE3), named BL21(LCD) and BL21(DCD). In the induced BL21(LCD) and BL21(DCD) compared with wild type, significantly higher H(2) S generation rates were observed. Additionally, higher survival rates, reduced contents of malondialdehyde (MDA) and hydrogen peroxide (H(2) O(2)), decreased activities of superoxide dismutase and catalase under 220 μmol l(-1) Cd stress were noted. We obtained similar results in the wild type treated with NaHS, a H(2) S donor. The above changes were substantially counteracted by the mixture of ammonia and pyruvic acid potassium (NH(3) + C(3) H(3) KO(3)), a synthetic inhibitor of H(2) S.
  
  
  CONCLUSIONS
  AtLCD and AtDCD catalyse the H(2) S production, generating an ameliorating effect against Cd-induced oxidative stress and resulting in E. coli resistance to Cd toxicity.
  
  
  CONCLUSIONS
  H(2) S as a gasotransmitter is certified to have an ameliorating effect against Cd toxicity, thus providing information for further research regarding the role of H(2) S in regulating resistance to the heavy metal stress in organisms.
authors: School of Life Science, Shanxi University, Taiyuan, China.
authors: J J
authors: JJ
authors: Shen
authors: Z J
authors: ZJ
authors: Qiao
authors: T J
authors: TJ
authors: Xing
authors: L P
authors: LP
authors: Zhang
authors: Y L
authors: YL
authors: Liang
authors: Z P
authors: ZP
authors: Jin
authors: G D
authors: GD
authors: Yang
authors: R
authors: R
authors: Wang
authors: Y X
authors: YX
authors: Pei
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D003001:Cloning, Molecular; D003542:Cystathionine gamma-Lyase / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D005822:Genetic Vectors; D006861:Hydrogen Peroxide / Q000032:analysis; D006862:Hydrogen Sulfide / Q000378:metabolism*; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013440:Sulfides; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; D013440:Sulfides; D002104:Cadmium; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D003542:Cystathionine gamma-Lyase; C025451:sodium bisulfide; D006862:Hydrogen Sulfide