title: Segregation of donor cell mitochondrial DNA in gaur-bovine interspecies somatic cell nuclear transfer embryos, fetuses and an offspring.
abstract: The fate of foreign mitochondrial DNA (mtDNA) following somatic cell nuclear transfer (SCNT) is still controversial. In this study, we examined the transmission of the heteroplasmic mtDNA of gaur donor cells and recipient bovine oocytes to an offspring and aborted and mummified fetuses at various levels during the development of gaur-bovine interspecies SCNT (iSCNT) embryos. High levels of the donor cell mtDNA were found in various tissue samples but they did not have any beneficial effect to the survival of iSCNT offspring. However, the factors on mtDNA inheritance are unique for each iSCNT experiment and depend on the recipient oocyte and donor cell used, which might play an important role in the efficiency of iSCNT.
authors: Embryo Technology and Stem Cell Research Center, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
authors: Sumeth
authors: S
authors: Imsoonthornruksa
authors: Kanokwan
authors: K
authors: Srirattana
authors: Wanwisa
authors: W
authors: Phewsoi
authors: Wanchai
authors: W
authors: Tunwattana
authors: Rangsun
authors: R
authors: Parnpai
authors: Mariena
authors: M
authors: Ketudat-Cairns
mesh_terms: D000818:Animals; D002417:Cattle; D019976:Cloning, Organism; D004272:DNA, Mitochondrial; D047108:Embryonic Development; D005260:Female; D005333:Fetus; D022761:Gene Transfer, Horizontal; D008297:Male; D053652:Nuclear Transfer Techniques; D012418:Ruminants / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial