title: Homogeneous electrochemical monitoring of exonuclease III activity and its application to nucleic acid testing by target recycling.
abstract: A simple and fast electrochemical nucleic acid assay based on a target recycling strategy and the release of a double-stranded DNA intercalating redox probe upon digestion of a specific duplex by exonuclease III is demonstrated.
authors: Laboratoire d'Electrochimie Moléculaire, UMR 7591 CNRS, Université Paris Diderot, Sorbonne Paris Cité, 15 rue Jean-Antoine de Baïf, F-75205 Paris Cedex 13, France.
authors: Rebeca
authors: R
authors: Miranda-Castro
authors: Damien
authors: D
authors: Marchal
authors: Benoît
authors: B
authors: Limoges
authors: François
authors: F
authors: Mavré
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D055664:Electrochemical Techniques; D005090:Exodeoxyribonucleases / Q000737:chemistry* / Q000378:metabolism; D007364:Intercalating Agents / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D012486:Salmonella typhimurium / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D007364:Intercalating Agents; D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III