title: Inhibition of acetate accumulation leads to enhanced production of (R,R)-2,3-butanediol from glycerol in Escherichia coli.
abstract: This work describes the production of (R,R)-2,3-butanediol in Escherichia coli using glycerol by metabolic engineering approaches. The introduction of a synthetic pathway converting pyruvate to (R,R)-2,3-butanediol into wild-type E. coli strain BW25113 led to the production of (R,R)-2,3-butanediol at a titer of 3.54 g/l and a yield of 0.131 g product/g glycerol (26.7 % of theoretical maximum) with acetate (around 3.00 g/l) as the dominant by-product. We therefore evaluated the impacts of deleting the genes ackA or/and poxB that are responsible for the major by-product, acetate. This increased production of (R,R)-2,3-butanediol to 9.54 g/l with a yield of 0.333 g product/g glycerol (68.0 % of theoretical maximum) in shake flask studies. The utilization of low-priced crude glycerol to produce value-added chemicals is of great significance to the economic viability of the biodiesel industry.
authors: Key Laboratory of Bioprocess of Beijing, Beijing University of Chemical Technology, Beijing 100029, China.
authors: Xiaolin
authors: X
authors: Shen
authors: Yuheng
authors: Y
authors: Lin
authors: Rachit
authors: R
authors: Jain
authors: Qipeng
authors: Q
authors: Yuan
authors: Yajun
authors: Y
authors: Yan
mesh_terms: D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid / Q000378:metabolism; D056804:Biofuels; D002072:Butylene Glycols / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000235:genetics; D005990:Glycerol / Q000378:metabolism*; D060847:Metabolic Engineering; D019289:Pyruvic Acid / Q000378:metabolism
chemical_list: D000085:Acetates; D056804:Biofuels; D002072:Butylene Glycols; D029968:Escherichia coli Proteins; C026978:2,3-butylene glycol; D019289:Pyruvic Acid; D005990:Glycerol; D019342:Acetic Acid