title: The alignment of MC3T3-E1 osteoblasts on steps of slip traces introduced by dislocation motion.
abstract: Bone tissue shows a highly anisotropic microstructure comprising biological apatite and collagen fibrils produced by the mutual activities of bone cells, which dominates its mechanical function. Accordingly, directional control of osteoblasts is crucial for forming anisotropic bone tissue. A new approach was proposed for controlling cell directionality by using crystallographic slip traces caused by dislocation glide. Dislocations were introduced into α-titanium single crystals by plastic deformation of (011¯0)[21¯1¯0] slip system, inducing a step-like structure with acute angles between the surface normal and the slip plane. Topographical properties of step patterning, including step interval and step height, could be controlled by varying the compressive plastic strain. The step geometry introduced by plastic deformation strongly influenced osteoblast elongation, and it aligned preferentially along slip traces. Ti substrates under 10% plastic strain with step height of approximately 300 nm and step interval of 10 μm induced osteoblast alignment most successfully. Actin stress fibers elongated parallel to slip traces, with polarized vinculin accumulation between steps.
authors: Department of Materials Science and Engineering, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
authors: Aira
authors: A
authors: Matsugaki
authors: Gento
authors: G
authors: Aramoto
authors: Takayoshi
authors: T
authors: Nakano
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D019245:Compressive Strength / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects*; D013194:Staining and Labeling; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*
chemical_list: D014025:Titanium