title: Bubble Jet agent release cartridge for chemical single cell stimulation.
abstract: We present a new method for the distinct specific chemical stimulation of single cells and small cell clusters within their natural environment. By single-drop release of chemical agents with droplets in size of typical cell diameters (d <30 μm) on-demand micro gradients can be generated for the specific manipulation of single cells. A single channel and a double channel agent release cartridge with integrated fluidic structures and integrated agent reservoirs are shown, tested, and compared in this publication. The single channel setup features a fluidic structure fabricated by anisotropic etching of silicon. To allow for simultaneous release of different agents even though maintaining the same device size, the second type comprises a double channel fluidic structure, fabricated by photolithographic patterning of TMMF. Dispensed droplet volumes are V = 15 pl and V = 10 pl for the silicon and the TMMF based setups, respectively. Utilizing the agent release cartridges, the application in biological assays was demonstrated by hormone-stimulated premature bud formation in Physcomitrella patens and the individual staining of one single L 929 cell within a confluent grown cell culture.
authors: BIOSS - Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany. nicolai.wangler@imtek.uni-freiburg.de
authors: N
authors: N
authors: Wangler
authors: M
authors: M
authors: Welsche
authors: M
authors: M
authors: Blazek
authors: M
authors: M
authors: Blessing
authors: M
authors: M
authors: Vervliet-Scheebaum
authors: R
authors: R
authors: Reski
authors: C
authors: C
authors: Müller
authors: H
authors: H
authors: Reinecke
authors: J
authors: J
authors: Steigert
authors: G
authors: G
authors: Roth
authors: R
authors: R
authors: Zengerle
authors: N
authors: N
authors: Paust
mesh_terms: D019068:Bryopsida / Q000166:cytology / Q000187:drug effects; D003583:Cytokinins / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D059010:Single-Cell Analysis / Q000295:instrumentation*
chemical_list: D003583:Cytokinins