title: MiR-30c: a novel regulator of salt tolerance in tilapia.
abstract: miRNAs comprise a class of ~22 nt noncoding RNAs that modulate the stability and/or translational potential of their mRNA targets. Emerging data suggest that stress conditions can alter the biogenesis of miRNAs, thereby changing the expression of mRNA targets. Here, we reveal that miR-30c, a kidney-enriched miRNA, emerges as a crucial osmoregulator in Nile tilapia. miR-30c loss of function leads to an inability to respond to osmotic stress. We identify HSP70 as one of the direct regulatory targets of miR-30c. miR-30c directly regulates HSP70 by targeting its 3'-UTR, and inhibition of miR-30c substantially increases HSP70 mRNA level in vivo. Taken together, our experiments suggest that miRNAs participate in a regulatory circuit that allows rapid gene program transitions in response to osmotic stress. miR-30c may be developed as a molecular marker to assist to breed or genetically engineer salt tolerant species.
authors: Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China.
authors: Biao
authors: B
authors: Yan
authors: Jin-Tao
authors: JT
authors: Guo
authors: Li-Hui
authors: LH
authors: Zhao
authors: Jin-Liang
authors: JL
authors: Zhao
mesh_terms: D000818:Animals; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D007668:Kidney / Q000502:physiology; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D009997:Osmotic Pressure; D055049:Salt Tolerance / Q000235:genetics*; D017210:Tilapia / Q000235:genetics / Q000502:physiology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D035683:MicroRNAs