title: Regulation of mammalian transcription by Gdown1 through a novel steric crosstalk revealed by cryo-EM.
abstract: In mammals, a distinct RNA polymerase II form, RNAPII(G) contains a novel subunit Gdown1 (encoded by POLR2M), which represses gene activation, only to be reversed by the multisubunit Mediator co-activator. Here, we employed single-particle cryo-electron microscopy (cryo-EM) to disclose the architectures of RNAPII(G), RNAPII and RNAPII in complex with the transcription initiation factor TFIIF, all to ~19 Å. Difference analysis mapped Gdown1 mostly to the RNAPII Rpb5 shelf-Rpb1 jaw, supported by antibody labelling experiments. These structural features correlate with the moderate increase in the efficiency of RNA chain elongation by RNAP II(G). In addition, our updated RNAPII-TFIIF map showed that TFIIF tethers multiple regions surrounding the DNA-binding cleft, in agreement with cross-linking and biochemical mapping. Gdown1's binding sites overlap extensively with those of TFIIF, with Gdown1 sterically excluding TFIIF from RNAPII, herein demonstrated by competition assays using size exclusion chromatography. In summary, our work establishes a structural basis for Gdown1 impeding initiation at promoters, by obstruction of TFIIF, accounting for an additional dependent role of Mediator in activated transcription.
authors: Institute of Chemistry, Academia Sinica, Taipei, Taiwan.
authors: Yi-Min
authors: YM
authors: Wu
authors: Jen-Wei
authors: JW
authors: Chang
authors: Chun-Hsiung
authors: CH
authors: Wang
authors: Yen-Chen
authors: YC
authors: Lin
authors: Pei-lun
authors: PL
authors: Wu
authors: Shih-hsin
authors: SH
authors: Huang
authors: Chia-Chi
authors: CC
authors: Chang
authors: Xiaopeng
authors: X
authors: Hu
authors: Averell
authors: A
authors: Gnatt
authors: Wei-hau
authors: WH
authors: Chang
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002850:Chromatography, Gel; D020285:Cryoelectron Microscopy; D011487:Protein Conformation; D012319:RNA Polymerase II / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D019473:Transcription Factors, TFII / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D014158:Transcription, Genetic
chemical_list: C433812:POLR2M protein, human; D011994:Recombinant Proteins; D019473:Transcription Factors, TFII; D012319:RNA Polymerase II; C056604:transcription factor TFIIF