title: Preparation and biological evaluation of chitosan-collagen-icariin composite scaffolds for neuronal regeneration.
abstract: In this study the chitosan/collagen/icariin composite scaffolds for nerve regeneration were produced by blending and crosslinking chitosan with collagen and icariin. The microstructure of the composite scaffolds was observed by scanning electron microscopy. 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide and attachment assays were conducted, respectively, to investigate the biocompatibility of the scaffolds. Cell cultural tests showed that the channel-structured porous scaffolds acted as a positive factor to support connective nerve cell growth. After culture, cells showed a clear flow trend to move close to the composite scaffolds in culture solution, arranging in spiral-like, and aligned parallel to the orientation of the channel structure on the surface of scaffolds. When compared to pure chitosan and chitosan/collagen scaffolds, Schwann cells and PC12 cells on the chitosan/collagen/icariin composite scaffolds exhibited the greatest proliferation and longest average neurite length. These results suggested that the chitosan/collagen/icariin composite scaffolds are potential cell carriers in nerve tissue engineering.
authors: Department of Materials Science and Engineering, Fujian University of Technology, Fuzhou 350108, China. milkhoney3@163.com
authors: Chun Rong
authors: CR
authors: Yang
authors: Jing Di
authors: JD
authors: Chen
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049108:Cell Enlargement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure; D016716:PC12 Cells; D051381:Rats; D012583:Schwann Cells / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000166:cytology; D013997:Time Factors; D054457:Tissue Scaffolds
chemical_list: D005419:Flavonoids; D003094:Collagen; D048271:Chitosan; C056599:icariin