title: Improved protocol for the extraction of bacterial mRNA from soils.
abstract: An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.
authors: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.
authors: Shilpi
authors: S
authors: Sharma
authors: Ravikumar
authors: R
authors: Mehta
authors: Rashi
authors: R
authors: Gupta
authors: Michael
authors: M
authors: Schloter
mesh_terms: D056186:Metagenomics / Q000379:methods*; D011387:Prokaryotic Cells; D012333:RNA, Messenger / Q000302:isolation & purification*; D012988:Soil Microbiology; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger