title: Detection of acetaldehyde derived N(2)-ethyl-2'-deoxyguanosine in human leukocyte DNA following alcohol consumption.
abstract: Epidemiological studies have shown an association between alcohol (ethanol) consumption and increased cancer risk. The effect of alcohol consumption on the levels and persistence of N(2)-ethylidene-2'-deoxyguanosine (N(2)-ethylidene-dG) formed by acetaldehyde, the oxidative metabolite of ethanol, in human leukocyte DNA was investigated. DNA was isolated from venous blood samples obtained from 30 male non-smoking individuals before consumption of alcohol (0h) and subsequently at 3-5h following the consumption of 150mL of vodka (containing 42% pure ethanol). Additional samples were collected 24h and 48h post-alcohol consumption. The levels of N(2)-ethyl-2'-deoxyguanosine (N(2)-ethyl-dG) in the DNA were determined following reduction of N(2)-ethylidene-dG with sodium cyanoborohydride using a liquid chromatography-tandem mass spectrometry selected reaction monitoring method. A slight time-dependent trend showing an increase and decrease in the levels of N(2)-ethyl-dG was observed following consumption of alcohol compared to time 0h, however, the differences were not statistically significant. The average levels of N(2)-ethyl-dG observed at 0h, 3-5h, 24h and 48h time points following ingestion of alcohol were 34.6±21.9, 35.1±21.0, 36.8±20.7 and 35.6±21.1 per 10(8) 2'-deoxynucleosides, respectively. In conclusion, alcohol consumption that could be encountered under social drinking conditions, does not significantly alter the levels of the acetaldehyde derived DNA adduct, N(2)-ethyl-dG in human leukocyte DNA from healthy individuals.
authors: Department of Cancer Studies and Molecular Medicine, University of Leicester, University Road, Leicester, UK. rs25@le.ac.uk
authors: Rajinder
authors: R
authors: Singh
authors: Jolanta
authors: J
authors: Gromadzinska
authors: Yogita
authors: Y
authors: Mistry
authors: Rebecca
authors: R
authors: Cordell
authors: Tina
authors: T
authors: Juren
authors: Dan
authors: D
authors: Segerbäck
authors: Peter B
authors: PB
authors: Farmer
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism*; D000328:Adult; D000428:Alcohol Drinking / Q000235:genetics* / Q000378:metabolism; D002853:Chromatography, Liquid; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000378:metabolism; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D007962:Leukocytes / Q000737:chemistry; D008297:Male; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D055815:Young Adult
chemical_list: D018736:DNA Adducts; C492934:N2-ethyl-2'-deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine; D000079:Acetaldehyde