title: Participation of microRNA 124-CREB pathway: a parallel memory enhancing mechanism of standardised extract of Bacopa monniera (BESEB CDRI-08).
abstract: Bacosides, the effective component of standardised leaf extract of Bacopa monniera (BESEB CDRI-08) has been reported to have memory enhancing effect. Our previous reports suggested that BESEB CDRI-08 (BME) improves memory in postnatal rats by enhancing serotonin [5-hydroxytryptamine (5-HT)] metabolism, its transportation and subsequently activates 5-HT(3A) receptor during hippocampus-dependent learning. In this study, we examine whether the up-regulated 5-HT(3A) receptor activity by BME modulate microRNA 124-CREB pathway to enhance synaptic plasticity. Wistar rat pups received single dose of vehicle solution (0.5 % gum acacia + 0.9 % saline)/BME (80 mg/kg)/mCPBG (10 mg/kg)/BME + mCPBG during the postnatal days (PND) 15-29. On PND 30, individuals were trained at brightness discrimination task and 24 h later, they were tested on the task. The BME treated group exhibited significantly lower percentage of errors during retention than acquisition. In addition, pre-miR-124 expression in hippocampus was significantly down-regulated in the BME and mCPBG + BME treated groups combined with a significant increase in the plasticity related genes, cAMP response element-binding protein, its phosphorylation and postsynaptic density protein 95. Our results suggest that this may be one of the mechanisms of bacosides present in BME for the memory enhancement.
authors: Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, India.
authors: Jayakumar
authors: J
authors: Preethi
authors: Hemant K
authors: HK
authors: Singh
authors: Prisila Dulcy
authors: PD
authors: Charles
authors: Koilmani Emmanuvel
authors: KE
authors: Rajan
mesh_terms: D000818:Animals; D034681:Bacopa / Q000737:chemistry*; D001483:Base Sequence; D015153:Blotting, Western; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D017931:DNA Primers; D006624:Hippocampus / Q000187:drug effects; D035683:MicroRNAs / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D017931:DNA Primers; C548144:MIRN124 microRNA, rat; D035683:MicroRNAs; D010936:Plant Extracts