title: Detection of cross infections by Leishmania spp. and Trypanosoma spp. in dogs using indirect immunoenzyme assay, indirect fluorescent antibody test and polymerase chain reaction.
abstract: The aim of this study was to detect cross infections by Leishmania spp. and Trypanosoma spp. using enzyme-linked immunosorbent assay (ELISA), indirect fluorescent antibody test (IFAT) and polymerase chain reaction (PCR). Thus, 408 blood samples were collected from dogs domiciled in Araçatuba Municipality, São Paulo State, Brazil; the dogs were of both sexes, of several breeds and aged 6 months. For Leishmania spp., 14.95% (61 out of 408) of dogs were reactive using IFAT. Positivity was 20.10% (82 out of 408) using ELISA and 29.66% (121 out of 408) using PCR, with significant differences for the sex and age of these animals (p < 0.05). For Trypanosoma spp., antibody occurrence using ELISA was 10.54% (43 out of 408), while PCR indicated 2.45% (10 out of 408) positive dogs. Using IFAT, 10.29% (42 out of 408) of animals were considered positive and only sex showed a significant difference (p < 0.05). In this study, 10.54% (43 out of 408) of animals were seropositive according to ELISA for Trypanosoma spp., of which 79.07% (34 out of 43) showed positive results in the molecular diagnosis for Leishmania spp., while of the 10.29% (42 out of 408) positive dogs according to IFAT, 95.24 % (40 out of 42) had confirmed infection by this parasite. The obtained results demonstrate evidence of cross infections by both protozoa in the animals analysed in this study.
authors: Faculdade de Odontologia, UNESP-Universidade Estadual Paulista, Rua Clóvis Pestana, 793, Araçatuba, São Paulo CEP 16050-680, Brazil.
authors: M A
authors: MA
authors: Viol
authors: V M F
authors: VM
authors: Lima
authors: M C C
authors: MC
authors: Aquino
authors: G
authors: G
authors: Gallo
authors: I P
authors: IP
authors: Alves
authors: D
authors: D
authors: Generoso
authors: S H V
authors: SH
authors: Perri
authors: S B
authors: SB
authors: Lucheis
authors: H
authors: H
authors: Langoni
authors: C M
authors: CM
authors: Nunes
authors: K D S
authors: KD
authors: Bresciani
mesh_terms: D000818:Animals; D001938:Brazil / Q000453:epidemiology; D060085:Coinfection / Q000453:epidemiology / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006801:Humans; D007891:Leishmania / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000150:complications / Q000453:epidemiology / Q000662:veterinary*; D008297:Male; D010274:Parasitology / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D014345:Trypanosoma / Q000302:isolation & purification*; D014352:Trypanosomiasis / Q000150:complications / Q000453:epidemiology / Q000662:veterinary*