title: Media composition: pH and buffers.
abstract: The proper pH of media is a crucial parameter for optimizing efficacy of gamete and embryo culture. Selecting the right media pH and stabilizing this pH are important variables in minimizing intracellular stress and optimizing development. Regulation of intracellular (pHi) and extracellular pH (pHe) is discussed, as well as methods to prevent harmful pHe oscillations. Furthermore, proper approaches to ensure accurate measurement of media pHe are described.
authors: University of Michigan, Ann Arbor, MI, USA. swainj@umich.edu
authors: Jason E
authors: JE
authors: Swain
mesh_terms: D000818:Animals; D002021:Buffers; D002138:Calibration; D003470:Culture Media / Q000737:chemistry*; D046149:Embryo Culture Techniques / Q000295:instrumentation / Q000379:methods*; D005110:Extracellular Space / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000737:chemistry; D051379:Mice
chemical_list: D002021:Buffers; D003470:Culture Media