title: Nanoporous silica microparticle interaction with toll-like receptor agonists in macrophages.
abstract: Nanoporous silica microparticles (NSiO(2)-MP) are considered to be potential drug delivery systems and scaffolding platforms in tissue engineering. However, few biocompatibility studies regarding NSiO(2)-MP interaction with the immune system have been reported. Toll-like receptors (TLR) are involved in host defence as well as autoimmune and inflammatory diseases. The results show that NSiO(2)-MP up to 100μgml(-1) do not affect macrophage cell viability after 24h cell culture. Moreover, NSiO(2)-MP do not compromise the cell viability of TLR-activated Raw 264.7 cells, for either cell surface TLR (TLR1/TLR2/TLR4/TLR6) or endocytic compartment TLR (TLR3/TLR7/TLR9). Furthermore, Raw 264.7 cells do not respond to NSiO(2)-MP exposure in terms of IL-6 or IL-10 secretion. NSiO(2)-MP co-treatment in the presence of TLR ligands does not impair or enhance the secretion of the pro-inflammatory cytokine IL-6 or the regulatory cytokine IL-10. Thus, NSiO(2)-MP do not affect macrophage polarization towards a pro-inflammatory or immunosuppressive status, representing added value in terms of biocompatibility compared with other SiO(2)-based micro- and nanoparticles.
authors: CABIMER-Andalusian Center for Molecular Biology and Regenerative Medicine (CSIC-University of Seville-UPO), Americo Vespucio s/n. Parque Cientifico y Tecnologico Cartuja 93, E-41092 Seville, Spain.
authors: M
authors: M
authors: Cejudo-Guillén
authors: M L
authors: ML
authors: Ramiro-Gutiérrez
authors: A
authors: A
authors: Labrador-Garrido
authors: A
authors: A
authors: Díaz-Cuenca
authors: D
authors: D
authors: Pozo
mesh_terms: D000818:Animals; D002460:Cell Line; D016503:Drug Delivery Systems; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism; D008024:Ligands; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D053758:Nanoparticles; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology*; D013997:Time Factors; D051193:Toll-Like Receptors / Q000819:agonists* / Q000276:immunology / Q000378:metabolism
chemical_list: C556062:IL10 protein, mouse; D015850:Interleukin-6; D008024:Ligands; D051193:Toll-Like Receptors; D016753:Interleukin-10; D012822:Silicon Dioxide