title: Comparative analysis of periprostatic implantation and intracavernosal injection of human adipose tissue-derived stem cells for erectile function recovery in a rat model of cavernous nerve injury.
abstract: BACKGROUND
  We compared periprostatic implantation (PPI) and intracavernosal injection (ICI) of human adipose tissue-derived stem cell (ADSC) to facilitate recovery of erectile function in a rat model of cavernous nerve (CN) injury.
  
  
  METHODS
  Bilateral CN dissection (BCND) was induced in Sprague-Dawley rats. After BCND 10 rats each were treated with PPI and/or ICI of ADSCs. After 4 weeks erectile responses to electric pelvic ganglion stimulation were studied. Each penis was evaluated in terms of the expression of neuronal nitric oxide synthase and smooth muscle content.
  
  
  RESULTS
  The ratio of maximal intracavernosal pressure to mean arterial pressure was significantly decreased in the BCND group (24.5%) compared to the sham group (64.2%). PPI and ICI significantly improved erectile function (46.7% and 47.9%, respectively) compared to the BCND group. A combination of PPI and ICI (42.5%) did not afford any incremental effect on erectile function. After stem cell therapy, the expression of neuronal nitric oxide synthase increased slightly in the ICI group without statistical relevance, whereas the PPI and combination groups showed marginally significant increases (P = 0.08). In both the PPI and ICI groups, the smooth muscle content was similar to the sham group. The combination group showed remarkable increase in smooth muscle content to an extent greater than that seen when either treatment was given alone, although statistically not significant.
  
  
  CONCLUSIONS
  PPI or ICI of ADSCs in a rat model of CN injury were equally effective in recovering penile erection, but may address different types of pathophysiology.
authors: Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
authors: Dalsan
authors: D
authors: You
authors: Myoung Jin
authors: MJ
authors: Jang
authors: Jiyeon
authors: J
authors: Lee
authors: Nayoung
authors: N
authors: Suh
authors: In Gab
authors: IG
authors: Jeong
authors: Dong Wan
authors: DW
authors: Sohn
authors: Sae Woong
authors: SW
authors: Kim
authors: Tai Young
authors: TY
authors: Ahn
authors: Choung-Soo
authors: CS
authors: Kim
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002478:Cells, Cultured; D004558:Electric Stimulation; D007172:Erectile Dysfunction / Q000209:etiology* / Q000503:physiopathology / Q000628:therapy*; D006801:Humans; D008297:Male; D023421:Models, Animal; D009130:Muscle, Smooth / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D010413:Penis / Q000294:innervation / Q000503:physiopathology; D059348:Peripheral Nerve Injuries / Q000150:complications* / Q000378:metabolism / Q000503:physiopathology; D011467:Prostate / Q000601:surgery*; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*
chemical_list: D052248:Nitric Oxide Synthase Type I