title: Delay in virus accumulation and low virus transmission from transgenic rice plants expressing Rice tungro spherical virus RNA.
abstract: Rice tungro, a devastating viral disease of rice in South and Southeast Asia, is caused by the joint infection of a DNA virus, Rice tungro bacilliform virus (RTBV) and an RNA virus Rice tungro spherical virus (RTSV). RTBV and RTSV are transmitted exclusively by the insect vector Green leafhopper (GLH). RTSV is necessary for the transmission of RTBV. To obtain transgenic resistance against RTSV, indica rice plants were transformed using DNA constructs designed to express an untranslatable sense or anti-sense RTSV RNA. Progeny of primary transformants showing low copies of the integrated transgenes and accumulating the corresponding transcripts at low levels were challenged with viruliferous GLH. Three out of four transgenic plant lines expressing untranslatable RTSV RNA in the sense orientation and two out of the four lines expressing an RTSV gene in the anti-sense orientation showed delayed buildup of RTSV RNA over time. Transmission of RTBV from the above lines was reduced significantly.
authors: Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
authors: Vidhu
authors: V
authors: Verma
authors: Shweta
authors: S
authors: Sharma
authors: S Vimla
authors: SV
authors: Devi
authors: S
authors: S
authors: Rajasubramaniam
authors: Indranil
authors: I
authors: Dasgupta
mesh_terms: D000818:Animals; D018562:Disease Transmission, Infectious / Q000517:prevention & control*; D015870:Gene Expression; D006430:Hemiptera / Q000821:virology; D012275:Oryza / Q000235:genetics / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified; D012367:RNA, Viral / Q000096:biosynthesis*; D052641:Tungrovirus / Q000472:pathogenicity; D019193:Waikavirus / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D012367:RNA, Viral