title: Downregulation of miR-126 induces angiogenesis and lymphangiogenesis by activation of VEGF-A in oral cancer.
abstract: BACKGROUND
  MicroRNA (miRNA)-126 (miR-126) is an endothelial-specific miRNA located within intron 7 of epidermal growth factor-like domain 7 (EGFL7). However, the role of miR-126 in cancer is controversial.
  
  
  METHODS
  We examined the function of miR-126 in oral squamous cell carcinoma (OSCC) cells. Furthermore, a series of 118 cases with OSCC were evaluated for the expression levels of miR-126.
  
  
  RESULTS
  MicroRNA-126 (miR-126) was associated with cell growth and regulation of vascular endothelial growth factor-A activity, and demethylation treatment increased expression levels of miR-126 and EGFL7 in OSCC cells. A significant association was found between miR-126 expression and tumour progression, nodal metastasis, vessel density, or poor prognosis in OSCC cases. In the multivariate analysis, decreased miR-126 expression was strongly correlated with disease-free survival.
  
  
  CONCLUSIONS
  The present results suggest that miR-126 might be a useful diagnostic and therapeutic target in OSCC.
authors: Department of Molecular Pathology, Nara Medical University School of Medicine, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan.
authors: T
authors: T
authors: Sasahira
authors: M
authors: M
authors: Kurihara
authors: U K
authors: UK
authors: Bhawal
authors: N
authors: N
authors: Ueda
authors: T
authors: T
authors: Shimomoto
authors: K
authors: K
authors: Yamamoto
authors: T
authors: T
authors: Kirita
authors: H
authors: H
authors: Kuniyasu
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002135:Calcium-Binding Proteins; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D015536:Down-Regulation; D066255:EGF Family of Proteins; D016228:Endothelial Growth Factors / Q000235:genetics; D005260:Female; D006801:Humans; D042583:Lymphangiogenesis / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics*; D009389:Neovascularization, Pathologic / Q000235:genetics*; D015533:Transcriptional Activation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D002135:Calcium-Binding Proteins; D066255:EGF Family of Proteins; C527877:EGFL7 protein, human; D016228:Endothelial Growth Factors; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A