title: In vivo evaluation of in situ polysaccharide based hydrogel for prevention of postoperative adhesion.
abstract: In this paper, the carboxymethyl chitosan/oxidized dextran hydrogel was developed and its potency application in the prevention of postoperative adhesion was investigated. The developed hydrogel showed porous and interconnected interior structure with pore size about 250 μm, which was sensitive to lysozymic solution (1.5 μg/ml) with almost complete degradation after 4 weeks of in vitro incubation. In vivo study suggested that the developed hydrogel showed the great capacity on the prevention of postoperative adhesions in rat model. According to the result of histopathological examination, it clearly showed that the mesothelial cell layer of abdominal wall and cecum were completely recovered after 7 days of surgery in 3% carboxymethyl chitosan/oxidized dextran hydrogel group, while obvious adhesion between abdominal wall and cecum was observed as treatment with saline solution or 3% carboxymethyl chitosan solution after 1 day of surgery. All these results suggested that the developed biodegradable hydrogel might have potential application in the prevention of postoperative adhesion.
authors: Department of Prosthodontics, School & Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, China.
authors: Weiwei
authors: W
authors: Lou
authors: Hualin
authors: H
authors: Zhang
authors: Jianfeng
authors: J
authors: Ma
authors: Dafeng
authors: D
authors: Zhang
authors: Chuantong
authors: C
authors: Liu
authors: Siqian
authors: S
authors: Wang
authors: Zhennan
authors: Z
authors: Deng
authors: Haihong
authors: H
authors: Xu
authors: Jinsong
authors: J
authors: Liu
mesh_terms: D000287:Administration, Topical; D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D048271:Chitosan / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D003911:Dextrans / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D015552:Injections, Intralesional; D008297:Male; D008954:Models, Biological; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000008:administration & dosage* / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions / Q000517:prevention & control*
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D011134:Polysaccharides; C514968:carboxymethyl-chitosan; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan; C014392:carboxymethyl dextran