title: Culture systems: sequential.
abstract: Methods for the culture of preimplantation human embryos evolved primarily from those used for mouse embryos. The initial method was usually culture in a single medium in microdrops of medium under oil for 2-3 days before transfer. Subsequently, extended culture over the whole preimplantation period was used. The debate at present is which system is best, a sequential series of media to accommodate changes in physiology and metabolism of the embryo from a 1-cell zygote to the differentiated blastocyst stage or a single-step culture regime using the same culture medium throughout the preimplantation period. Aspects of the advantages and disadvantages of these two culture systems will be discussed.
authors: Research and Development, Sage IVF, A CooperSurgical Company, Redmond, OR, USA. Patrick.quinn@coopersurgical.com
authors: Patrick
authors: P
authors: Quinn
mesh_terms: D000818:Animals; D003470:Culture Media / Q000737:chemistry; D046149:Embryo Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: D003470:Culture Media