title: Construction of an infectious cDNA clone and gene expression vector of Tobacco vein banding mosaic virus (genus Potyvirus).
abstract: Tobacco vein banding mosaic virus (TVBMV, genus Potyvirus) mainly infects solanaceous plants and is of increasing economic importance in China. Here, we report sequence determination of the full-length 5'-untranslated region of TVBMV isolate HN39 and construction of an infectious clone. The resultant clone, pTVBMV, which was stabilized by introducing three introns in the P3 and CI-encoding regions, induced similar disease symptoms and accumulated similar titers of virus in plants of Nicotiana benthamiana, Nicotiana tabacum and N. rustica as the wild type HN39 isolate. Mutation of arginine to isoleucine (R182I) or aspartic acid to lysine (D198K) in HC-Pro alleviated the symptoms of pTVBMV significantly, indicating a role of the two amino acids in regulating virulence of TVBMV. The Aequoria victoriae gene for green fluorescent protein was inserted between the NIb and CP encoding regions of pTVBMV and expressed stably in the systemically infected N. benthamiana leaves, indicating suitability of pTVBMV for expression of foreign proteins in plants.
authors: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors: Rui
authors: R
authors: Gao
authors: Yan-Ping
authors: YP
authors: Tian
authors: Jie
authors: J
authors: Wang
authors: Xiao
authors: X
authors: Yin
authors: Xiang-Dong
authors: XD
authors: Li
authors: Jari P T
authors: JP
authors: Valkonen
mesh_terms: D002681:China; D003546:Cysteine Endopeptidases / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000235:genetics; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins; D037521:Virulence Factors; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus