title: Quantifying the effect of covalently immobilized enzymes on biofilm formation by atomic force microscopy-based single-cell force spectroscopy.
abstract: A novel atomic force microscopy-based single-cell force spectroscopy assay to quantify the adhesion of bacterial cells to surfaces was developed. The assay was applied to quantify the effect of two biofilm-degrading enzymes, the protease Subtilisin A and glycoside hydrolase cellulase, on the attachment of the biofilm-forming bacterial strain Cobetia marina. Insights on the mechanism of the initial adhesion and on the nature of the adhesion-mediating molecules were gained. The assay can be easily adapted to various other substrates, different bacterial strains and other fouling species (e.g., algae and diatoms).
authors: Leibniz Institute of Polymer Research Dresden, Institute for Biofunctional Polymer Materials, Hohe Str 6, 01069 Dresden, Germany.
authors: Jens
authors: J
authors: Friedrichs
authors: Andrea
authors: A
authors: Zieris
authors: Silvana
authors: S
authors: Prokoph
authors: Carsten
authors: C
authors: Werner
mesh_terms: D018441:Biofilms; D057219:Biofouling; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D020563:Gammaproteobacteria / Q000502:physiology; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D013381:Subtilisins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D006026:Glycoside Hydrolases; D013381:Subtilisins