title: Investigation of proNT/NMN secretion from small cell lung carcinoma             cells using a mouse xenograft model.
abstract: Proneurotensin/neuromedin N (proNT/NMN), the precursor of neurotensin (NT)             and neuromedin N (NMN), is produced by cancer tissues derived from the pancreas             and colon. NT stimulates tumor growth and proliferation through its receptors;             however, little is known about the precursor molecule in cancer tissues. We previously             demonstrated that proNT/NMN is secreted from small cell lung carcinoma (SCLC)             cell lines in serum-free conditioned medium, but not from non-small cell lung             carcinoma (NSCLC) cell lines. It was suggested that this precursor may serve as             a tumor marker for SCLC. In this study, we established in vivo xenograft models             to evaluate the possibility of proNT/NMN as a specific tumor marker. SBC3 cells,             derived from human SCLC, were inoculated into mice, and the proNT/NMN levels in             plasma and tumor tissues were detected using specific antibodies. In contrast             to control mouse plasma, the proNT/NMN levels in tumor-bearing mice increased             as the tumors grew, and the elevated plasma proNT/NMN levels were decreased by             tumor resection. Moreover, proNT/NMN was expressed in SBC3 tumors, suggesting             that proNT/NMN was secreted into blood from the tumor, and this secretion may             be specific to SCLC.
authors: Medical Genetics Division, Experimental Animal Facility, Shizuoka Cancer Center Research Institute, Shizuoka, Japan.
authors: Kanako
authors: K
authors: Wakabayashi-Nakao
authors: Koji
authors: K
authors: Maruyama
authors: Hidee
authors: H
authors: Ishii
authors: Koji
authors: K
authors: Muramatsu
authors: Keiichi
authors: K
authors: Hatakeyama
authors: Keiichi
authors: K
authors: Ohshima
authors: Shun-Ichiro
authors: S
authors: Ogura
authors: Takashi
authors: T
authors: Nakajima
authors: Ken
authors: K
authors: Yamaguchi
authors: Tohru
authors: T
authors: Mochizuki
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009496:Neurotensin / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D010446:Peptide Fragments / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D011498:Protein Precursors / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D055752:Small Cell Lung Carcinoma / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D010446:Peptide Fragments; D011498:Protein Precursors; C411471:proneurotensin; C042090:neuromedin N; D009496:Neurotensin