title: Endothelial microparticles and the antiangiogenic state in preeclampsia and the postpartum period.
abstract: OBJECTIVE
  We sought to determine if endothelial microparticles (EMPs), markers of endothelial damage, are associated with soluble fms-like tyrosine kinase 1 (sFlt1), soluble endoglin, and placental growth factor (PlGF) in women with preeclampsia.
  
  
  METHODS
  A prospective cohort study was conducted on 20 preeclamptic women and 20 controls. EMPs by flow cytometry, sFlt1, soluble endoglin, and PlGF were measured at time of enrollment, 48-hours postpartum, and 1-week postpartum.
  
  
  RESULTS
  Preeclamptic CD31(+)/42(-), CD62E(+), and CD105(+) EMP levels were significantly elevated in preeclamptics vs controls at time of enrollment. The sFlt1:PlGF ratio was correlated with CD31(+)/42(-) and CD105(+) EMPs (r = 0.69 and r = 0.51, respectively) in preeclampsia. Levels of CD31(+)/42(-) EMPs remained elevated 1-week postpartum (P = .026).
  
  
  CONCLUSIONS
  EMPs are elevated in preeclampsia. The correlation of EMPs and the sFlt1:PlGF ratio suggests that antiangiogenesis is related to apoptosis of the endothelia. Endothelial damage persists 1 week after delivery.
authors: Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center at Dallas, USA.
authors: Loren
authors: L
authors: Petrozella
authors: Mala
authors: M
authors: Mahendroo
authors: Brenda
authors: B
authors: Timmons
authors: Scott
authors: S
authors: Roberts
authors: Donald
authors: D
authors: McIntire
authors: James M
authors: JM
authors: Alexander
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000097:blood; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles / Q000502:physiology*; D000071063:Endoglin; D042783:Endothelial Cells / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D000072483:Placenta Growth Factor; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D049590:Postpartum Period / Q000097:blood*; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood; D011446:Prospective Studies; D011956:Receptors, Cell Surface / Q000097:blood; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; C000608036:PGF protein, human; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011257:Pregnancy Proteins; D011956:Receptors, Cell Surface; D000072483:Placenta Growth Factor; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1