title: Gss1 protein of the methylotrophic yeast Pichia pastoris is involved in glucose sensing, pexophagy and catabolite repression.
abstract: In the yeast Saccharomyces cerevisiae, the one-at-a-time deletions of either the high-affinity glucose sensor gene SNF3 or the low-affinity glucose sensor gene RGT2 only slightly reduced pexophagy; however, deleting both genes greatly reduced pexophagy, evincing interaction beyond the sum of the additive effects, as recently shown. The present study identifies the only ScSNF3/RGT2 ortholog in the methylotrophic yeast Pichia pastoris (designated as PpGSS1, from GlucoSe Sensor) and describes its roles in autophagic pathways (non-selective and selective). GSS1 knock-out strain has been constructed. The experiments support the hypothesis that Gss1 plays an important role in autophagic degradation of peroxisomes and glucose catabolite repression in P. pastoris.
authors: Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine.
authors: Andriy S
authors: AS
authors: Polupanov
authors: Volodymyr Y
authors: VY
authors: Nazarko
authors: Andriy A
authors: AA
authors: Sibirny
mesh_terms: D000595:Amino Acid Sequence; D001343:Autophagy / Q000187:drug effects*; D015139:Blotting, Southern; D057465:Catabolite Repression / Q000187:drug effects* / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D017353:Gene Deletion; D005800:Genes, Fungal / Q000235:genetics; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D000432:Methanol / Q000494:pharmacology*; D008969:Molecular Sequence Data; D019301:Oleic Acid / Q000494:pharmacology; D020675:Peroxisomes / Q000187:drug effects / Q000378:metabolism*; D010843:Pichia / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D019301:Oleic Acid; D005947:Glucose; D000432:Methanol