title: Comprehensive phenotyping of salt-induced hypertensive heart disease in living mice using cardiac magnetic resonance.
abstract: OBJECTIVE
  To characterise the effects of high-salt diet (HSD) on left ventricular (LV) mass, systolic function and coronary reserve in living mice using cardiac magnetic resonance imaging (MRI).
  
  
  METHODS
  Thirty C57BL/6 1-month-old female mice were fed either a control (n = 15) or an HSD (n = 15). After 3 months, LV volumes, ejection fraction and mass were assessed using time-resolved three-dimensional (3D) black-blood manganese-enhanced MRI, and coronary flow velocity reserve (CFVR) was assessed using dynamic MR angiography at rest and during adenosine-induced hyperaemia. Hearts were excised to assess LV wet mass and micro-vascular remodelling at histology.
  
  
  RESULTS
  Micro-vascular remodelling was found at histology in all investigated hearts from the HSD group and none from the control group. No difference between the HSD and control groups was found in terms of heart weight, LV volumes and ejection fraction. Heart to body weight ratio was higher in the HSD group (4.39 ± 0.24 vs 4.02 ± 0.16 mg/g, P < 0.001), because of lower body weight (22.3 ± 0.9 vs 24.0 ± 1.4 g, P < 0.001). CFVR was lower in the HSD group (1.73 ± 0.11 vs 1.94 ± 0.12, P < 0.001).
  
  
  CONCLUSIONS
  Phenotyping of hypertensive heart disease is feasible in living mice using dynamic MR angiography and time-resolved 3D black-blood manganese-enhanced MRI. HSD is associated with early impairment of coronary reserve, before the onset of significant hypertrophy.
authors: CHU/Université de Bordeaux-Institut LIRYC/INSERM U1045, Pessac, France. hcochet@wanadoo.fr
authors: Hubert
authors: H
authors: Cochet
authors: William
authors: W
authors: Lefrançois
authors: Michel
authors: M
authors: Montaudon
authors: François
authors: F
authors: Laurent
authors: Line
authors: L
authors: Pourtau
authors: Sylvain
authors: S
authors: Miraux
authors: Elodie
authors: E
authors: Parzy
authors: Jean-Michel
authors: JM
authors: Franconi
authors: Eric
authors: E
authors: Thiaudière
mesh_terms: D000818:Animals; D003326:Coronary Circulation / Q000502:physiology; D004195:Disease Models, Animal; D005260:Female; D006973:Hypertension / Q000139:chemically induced / Q000175:diagnosis*; D021621:Imaging, Three-Dimensional; D018810:Magnetic Resonance Angiography; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011897:Random Allocation; D012016:Reference Values; D018570:Risk Assessment; D017673:Sodium Chloride, Dietary; D013318:Stroke Volume / Q000502:physiology; D018487:Ventricular Dysfunction, Left / Q000139:chemically induced / Q000175:diagnosis / Q000503:physiopathology
chemical_list: D017673:Sodium Chloride, Dietary