title: Exploring the pan-surfome of Streptococcus suis: looking for common protein antigens.
abstract: Streptococcus suis is a swine and human pathogen for which no commercial vaccine is still available. Conserved and broadly distributed surface proteins have become the chosen targets for the development of efficacious vaccines that could overcome the problems of non-heterologous protection of bacterins or capsule polysaccharide-based vaccines. In this work, we have analyzed by proteomics a collection of 39 strains obtained from infected pigs. The isolates belonged to 19 of the most prevalent serotypes during the last years. We have applied the "shaving" approach to define the "pan-surfome" or the set of both common and unique surface proteins identified in such strains. This set was constituted by 113 proteins. We have categorized them for their potential for further vaccination studies, based on their distribution among strains and their a priori accessibility to antibodies. According to these criteria, the cell-wall protein SsnA appears to be the best candidate from this list, as it was that with the widest distribution among the analyzed pathogen types, it showed to be highly immunogenic and highly accessible to antibodies, as demonstrated by flow cytometry.
authors: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Campus de Excelencia Internacional CeiA3, and Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba, Spain.
authors: Lidia
authors: L
authors: Gómez-Gascón
authors: Inmaculada
authors: I
authors: Luque
authors: Alfonso
authors: A
authors: Olaya-Abril
authors: Irene
authors: I
authors: Jiménez-Munguía
authors: Ricardo A
authors: RA
authors: Orbegozo-Medina
authors: Esther
authors: E
authors: Peralbo
authors: Carmen
authors: C
authors: Tarradas
authors: Manuel J
authors: MJ
authors: Rodríguez-Ortega
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000235:genetics / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D003851:Deoxyribonucleases / Q000235:genetics / Q000276:immunology*; D005260:Female; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D013290:Streptococcal Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D022541:Streptococcal Vaccines / Q000276:immunology; D017011:Streptococcus suis / Q000235:genetics / Q000276:immunology*; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins; D022541:Streptococcal Vaccines; D003851:Deoxyribonucleases