title: Canonical Wnt signaling is required for Panax notoginseng saponin-mediated attenuation of the RANKL/OPG ratio in bone marrow stromal cells during osteogenic differentiation.
abstract: Panax notoginseng saponins (PNS) are known to regulate the osteogenic differentiation of bone marrow stromal cells (BMSCs). In the present study, we investigated whether PNS could promote the osteogenic differentiation of BMSCs through modulating the Wnt/β-catenin signaling pathways, which are implicated in BMSCs osteogenesis. We found that PNS enhanced the mRNA expression of OPG, β-catenin, and cyclin D1 while decreased the mRNA expression of RANKL and PPARγ2. The actions of PNS on BMSCs were reversed (or partially) by DKK-1, a classical inhibitor of Wnt/β-catenin signaling. These results suggest that PNS stimulating bone formation by promoting the proliferation and osteogenic differentiation of BMSCs, and could also protect the skeletal system by decreasing bone resorption through reduction of RANKL/OPG expression via Wnt/β-catenin signaling pathways.
authors: Department of Orthopaedics, First Affiliated Hospital, Shantou University Medical College, 57 Chang Ping Road, Shantou, Guangdong 515041, China.
authors: Bin
authors: B
authors: Chen
authors: Xue-dong
authors: XD
authors: Li
authors: Dong-xin
authors: DX
authors: Liu
authors: Hu
authors: H
authors: Wang
authors: Peng
authors: P
authors: Xie
authors: Zhao-yong
authors: ZY
authors: Liu
authors: Guo-qing
authors: GQ
authors: Hou
authors: Bo
authors: B
authors: Chang
authors: Shi-xin
authors: SX
authors: Du
mesh_terms: D000818:Animals; D001862:Bone Resorption; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D036145:Ginsenosides / Q000737:chemistry / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects; D053244:Osteoprotegerin / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics; D053723:Panax notoginseng / Q000737:chemistry*; D053245:RANK Ligand / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D051153:Wnt Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D060449:Wnt Signaling Pathway / Q000187:drug effects*
chemical_list: C531384:Dkk1 protein, rat; D036145:Ginsenosides; D036341:Intercellular Signaling Peptides and Proteins; D053244:Osteoprotegerin; D047495:PPAR gamma; D053245:RANK Ligand; D012333:RNA, Messenger; D051153:Wnt Proteins; D002118:Calcium