title: Clostridium difficile DNA polymerase IIIC: basis for activity of antibacterial compounds.
abstract: Based on the finding that aerobic Gram-positive antibacterials that inhibit DNA polymerase IIIC (pol IIIC) were potent inhibitors of the growth of anaerobic Clostridium difficile (CD) strains, we chose to clone and express the gene for pol IIIC from this organism. The properties of the recombinant enzyme are similar to those of related pol IIICs from Gram-positive aerobes, e.g. B. subtilis. Inhibitors of the CD enzyme also inhibited B. subtilis pol IIIC, and were competitive with respect to the cognate substrate 2'-deoxyguanosine 5'-triphosphate (dGTP). Significantly, several of these inhibitors of the CD pol IIIC had potent activity against the growth of CD clinical isolates in culture.
authors: Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, I-27100 Pavia, Italy.
authors: Andrea
authors: A
authors: Torti
authors: Andrea
authors: A
authors: Lossani
authors: Lida
authors: L
authors: Savi
authors: Federico
authors: F
authors: Focher
authors: George Edward
authors: GE
authors: Wright
authors: Neal Curtis
authors: NC
authors: Brown
authors: Wei-Chu
authors: WC
authors: Xu