title: A synthetic approach reveals extensive tunability of auxin signaling.
abstract: Explaining how the small molecule auxin triggers diverse yet specific responses is a long-standing challenge in plant biology. An essential step in auxin response is the degradation of Auxin/Indole-3-Acetic Acid (Aux/IAA, referred to hereafter as IAA) repressor proteins through interaction with auxin receptors. To systematically characterize diversity in degradation behaviors among IAA|receptor pairs, we engineered auxin-induced degradation of plant IAA proteins in yeast (Saccharomyces cerevisiae). We found that IAA degradation dynamics vary widely, depending on which receptor is present, and are not encoded solely by the degron-containing domain II. To facilitate this and future studies, we identified a mathematical model able to quantitatively describe IAA degradation behavior in a single parameter. Together, our results demonstrate the remarkable tunability conferred by specific configurations of the auxin response pathway.
authors: Department of Electrical Engineering , University of Washington, Seattle, Washington 98195, USA.
authors: Kyle A
authors: KA
authors: Havens
authors: Jessica M
authors: JM
authors: Guseman
authors: Seunghee S
authors: SS
authors: Jang
authors: Edith
authors: E
authors: Pierre-Jerome
authors: Nick
authors: N
authors: Bolten
authors: Eric
authors: E
authors: Klavins
authors: Jennifer L
authors: JL
authors: Nemhauser
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D005434:Flow Cytometry; D006207:Half-Life; D007210:Indoleacetic Acids / Q000378:metabolism* / Q000494:pharmacology; D008954:Models, Biological; D010937:Plant Growth Regulators / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D059748:Proteolysis; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013045:Species Specificity; D013997:Time Factors; D014170:Transformation, Genetic; D054875:Ubiquitination
chemical_list: D029681:Arabidopsis Proteins; D044783:F-Box Proteins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011956:Receptors, Cell Surface; D012097:Repressor Proteins; C501405:TIR1 protein, Arabidopsis; C030737:indoleacetic acid