title: Preparation, characterization, and process performance of composite fibrous adsorbents as cation exchangers for high throughput and high capacity bioseparations.
abstract: Fibrous materials are proposed as novel chromatographic supports depicting high throughput and high product capacity. In this work, a composite fiber harboring strong cation-exchange moieties has been investigated. Such materials were characterized by a plethora of physical methods including degree of swelling (DS), scanning electron microscope (SEM), confocal laser scanning microscopy (CLSM), and Fourier transform infrared spectroscopy-attenuated total reflection (FTIR-ATR). The composite showed a high degree of grafting (∼30%) and exhibited a high swelling ratio (∼300%). Moreover, homogenous grafting and the development of an internal (functional) hydrogel were observed. The fibrous adsorbent was packed utilizing a designed "double roll" supported-structure and subsequently tested for packing efficiency and chromatography performance. The mentioned system showed similar packing efficiency of height equivalent to a theoretical plate (HETP) value and higher permeability coefficient (0.92 × 10⁻⁷ cm²) than commercial resins. Experimentally determined Peclet number (Pe) values were within the range 60-90, suggesting a close-to-plug-flow condition. Total ionic capacity of the fibrous adsorbent was determined by the transition pH method. A capacity of 6.5 mequiv./g was obtained. Moreover, a high dynamic binding capacity for lysozyme was found to be 283 mg/g. On the other hand, a bed of randomly packed fiber also demonstrated high-resolution ability when a mixture of model protein was utilized to that end. Resolution was maintained at high flow rates (up to 900 cm/h) and utilizing shorter gradient development routines. Direct sequestration of a model protein (lysozyme) was also possible from an artificial mixture containing 1.5% yeast homogenate. Summarizing, the composite fibrous adsorbents exhibited superior performance during early protein capture and intermediate-resolution applications.
authors: Downstream Processing Laboratory, Jacobs University Bremen gGmbH, Campus Ring 1, D-28759 Bremen, Germany.
authors: Poondi Rajesh
authors: PR
authors: Gavara
authors: Rosa
authors: R
authors: Cabrera
authors: Rami Reddy
authors: RR
authors: Vennapusa
authors: Mariano
authors: M
authors: Grasselli
authors: Marcelo
authors: M
authors: Fernandez-Lahore
mesh_terms: D000327:Adsorption; D002411:Cation Exchange Resins / Q000737:chemistry*; D002412:Cations; D002852:Chromatography, Ion Exchange / Q000295:instrumentation*; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D010539:Permeability; D011312:Pressure; D011485:Protein Binding; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002411:Cation Exchange Resins; D002412:Cations; D007074:Immunoglobulin G; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D009113:Muramidase