title: Effects of ADHD therapeutic agents, methylphenidate and atomoxetine, on hippocampal neurogenesis in the adolescent mouse dentate gyrus.
abstract: Methylphenidate (MPH) and atomoxetine (ATX) are commonly used as attention-deficit/hyperactivity disorder (ADHD) therapeutic agents. In the present study, we investigated the effects of MPH and ATX on cell proliferation and neuronal differentiation in the dentate gyrus (DG) of the adolescent mouse by 5-bromo-2'-deoxyuridine (BrdU) and doublecortin (DCX) immunohistochemistry. BrdU-positive ((+)) cells, DCX(+) cells and BrdU(+)/NeuN(+) neurons (BrdU(+) cells with NeuN immunoreaction) were easily detected in the subgranular zone (SGZ) of the DG in the vehicle-, MPH- and ATX-treated groups. Among them, only in the 10mg/kg MPH-treated group, the numbers of BrdU(+), DCX(+) and BrdU(+)/NeuN(+) cells were significantly increased compared to those in the vehicle-treated group. In addition, brain-derived neurotrophic factor (BDNF) level was significantly increased in 10mg/kg MPH-treated group, not in the other experimental groups, compared to the vehicle-treated group. These results indicate that MPH, not ATX, can enhance cell proliferation and neuroblast differentiation in the SGZ of the DG via increasing BDNF level.
authors: Department of Emergency Medicine, Chuncheon Sacred Heart Hospital, College of Medicine, Hallym University, Chuncheon 200-702, South Korea.
authors: Tae Hun
authors: TH
authors: Lee
authors: Choong Hyun
authors: CH
authors: Lee
authors: In Hye
authors: IH
authors: Kim
authors: Bing Chun
authors: BC
authors: Yan
authors: Joon Ha
authors: JH
authors: Park
authors: Seung-Hae
authors: SH
authors: Kwon
authors: Ok Kyu
authors: OK
authors: Park
authors: Ji Hyeon
authors: JH
authors: Ahn
authors: Jun Hwi
authors: JH
authors: Cho
authors: Moo-Ho
authors: MH
authors: Won
authors: Sung Koo
authors: SK
authors: Kim
mesh_terms: D000818:Animals; D000069445:Atomoxetine Hydrochloride; D001289:Attention Deficit Disorder with Hyperactivity / Q000188:drug therapy; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D018891:Dentate Gyrus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000089662:Doublecortin Protein; D007150:Immunohistochemistry; D008297:Male; D008774:Methylphenidate / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D011437:Propylamines / Q000494:pharmacology*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; C000714847:Dcx protein, mouse; D000089662:Doublecortin Protein; D011437:Propylamines; D008774:Methylphenidate; D000069445:Atomoxetine Hydrochloride