title: PKCα suppresses 7,12-dimethylbenz[a]anthracene-induced skin tumor formation.
abstract: BACKGROUND
  Protein kinase C (PKC)α is distributed in almost all tissues and participates in various signaling pathways. However, the role of PKCα in carcinogenesis remains unclear. In this study, we performed complete skin carcinogenesis in PKCα knockout mice by repeated administration of 7,12-dimethylbenz[a]anthracene (DMBA).
  
  
  METHODS
  Complete skin carcinogenesis was performed by repeated DMBA treatment using PKCα knockout mice. The number of tumors was determined weekly. Tumor types were determined by Hematoxylin and eosin (H & E) analysis. Tumor growth was assayed by proliferating cell nuclear antigen (PCNA) staining.
  
  
  RESULTS
  In the knockout mice, the average number of tumors was 16.6/mouse at 20 weeks. In contrast, in the wild-type (WT) mice, the tumor number was 6.9/mouse. Growth and malignant grade of tumors in PKCα knockout mice did not differ from those in WT mice.
  
  
  CONCLUSIONS
  PKCα suppresses tumor formation, but not tumor growth and progression in skin carcinogenesis.
authors: Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
authors: Takeshi
authors: T
authors: Hara
authors: Shoji
authors: S
authors: Matsumura
authors: Fumihiko
authors: F
authors: Hakuno
authors: Shin-Ichiro
authors: S
authors: Takahashi
authors: Kazuhiro
authors: K
authors: Chida
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism*; D005260:Female; D051379:Mice; D018345:Mice, Knockout; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D012878:Skin Neoplasms / Q000139:chemically induced / Q000201:enzymology* / Q000235:genetics
chemical_list: D002273:Carcinogens; D015127:9,10-Dimethyl-1,2-benzanthracene; D051571:Protein Kinase C-alpha