title: Alpha-mangostin suppresses MMP-2 and MMP-9 expression in head and neck squamous carcinoma cells.
abstract: The purpose of this study was to investigate the effect of alpha-mangostin on matrix metalloproteinase (MMP)-2 and MMP-9 expression in head and neck squamous cell carcinoma (HNSCC). The human HNSCC cell lines were treated with alpha-mangostin and the cytotoxicity of alpha-mangostin in HNSCC was determined using the MTS assay. To determine the effect of alpha-mangostin on the expression of MMP-2 and MMP-9 in HNSCC, gelatin zymography and RT-PCR were performed. The results showed that alpha-mangostin increased in growth inhibition of HNSCC cell lines in a concentration-dependent manner. Treatment with alpha-mangostin decreased MMP-2 and MMP-9 expression in a concentration-dependent manner in all cell lines. These findings suggested that alpha-mangostin might be a potential therapeutic agent for HNSCC.
authors: Department of Oral Diagnosis, Faculty of Dentistry, Naresuan University, Phitsanulok, 65000, Thailand, ruchadapornk@yahoo.com.
authors: Ruchadaporn
authors: R
authors: Kaomongkolgit
mesh_terms: D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D002460:Cell Line; D017931:DNA Primers; D006258:Head and Neck Neoplasms / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044004:Xanthones / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D044004:Xanthones; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C021053:mangostin