title: Biosynthetic origin of the carbon skeleton of a novel anti-tumor compound, haloroquinone, from a marine-derived fungus, Halorosellinia sp.
abstract: The biosynthetic pathway of a new antitumor compound, haloroquinone, is elucidated to facilitate metabolic regulation for product accumulation and modification to produce new bioactive structural analogues of the compound.                 The biosynthetic origin of a novel promising protein kinase B inhibitor and anti-tumor compound, haloroquinone, from a marine-derived fungus, Halorosellinia sp. was clarified. The origin of carbon skeleton of haloroquinone was elucidated by feeding experiments with [2-(13)C]malonate and [1,2,3-(13)C(3)]malonate followed by (13)C-NMR analysis of the isolated compounds: 15 carbon atoms were derived from malonate, of which eight were from the methylene group and seven from the carboxyl group. The remaining one is probably obtained by O-methylation. Haloroquinone is thus synthesized via a polyketide pathway using malonyl-CoA as both the starter unit and the extender unit.
authors: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. niu1206@163.com
authors: Chuanpeng
authors: C
authors: Niu
authors: Menghao
authors: M
authors: Cai
authors: Yuanxing
authors: Y
authors: Zhang
authors: Xiangshan
authors: X
authors: Zhou
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000378:metabolism; D002247:Carbon Isotopes / Q000737:chemistry / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008958:Models, Molecular; D047428:Protein Kinase Inhibitors / Q000737:chemistry* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors; D011809:Quinones / Q000737:chemistry* / Q000378:metabolism*; D013098:Xylariales / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D002247:Carbon Isotopes; D047428:Protein Kinase Inhibitors; D011809:Quinones; D051057:Proto-Oncogene Proteins c-akt