title: Saccharification of poplar biomass by using lignocellulases from Pholiota adiposa.
abstract: A basidiomycetous fungus, identified as Pholiota adiposa SKU0714 on the basis of morphological and phylogenetic analyses, was found to secrete efficient lignocellulose degrading enzymes. The strain showed maximum endoglucanase, cellobiohydrolase and β-glucosidase activities of 26, 32 and 39 U/mL, respectively and also secreted xylanase, laccase, mannanase, and lignin peroxidase with activities of 1680, 0.12, 65 and 0.41 U/mL, respectively when grown with rice straw as a carbon source. Among the various plant biomasses tested for saccharification, poplar biomass produced the maximum amount of reducing sugar. Response surface methodology was used to optimize hydrolysis parameters. A maximum saccharification yield of 83.4% (667 mg/g-substrate), the highest yield from any plant biomass, was obtained with Populus biomass after 24h of hydrolysis. P. adiposa was proven to be a good choice for the production of reducing sugars from cellulosic biomass.
authors: Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
authors: Sujit Sadashiv
authors: SS
authors: Jagtap
authors: Saurabh Sudha
authors: SS
authors: Dhiman
authors: Marimuthu
authors: M
authors: Jeya
authors: Yun Chan
authors: YC
authors: Kang
authors: Joon-Ho
authors: JH
authors: Choi
authors: Jung-Kul
authors: JK
authors: Lee
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D002480:Cellulase / Q000378:metabolism*; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000494:pharmacology; D055368:Pholiota / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D010802:Phylogeny; D032107:Populus / Q000187:drug effects / Q000378:metabolism*; D012044:Regression Analysis; D015203:Reproducibility of Results; D013501:Surface-Active Agents / Q000494:pharmacology; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D013501:Surface-Active Agents; C036909:lignocellulose; D002244:Carbon; D008031:Lignin; D001617:beta-Glucosidase; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; D009584:Nitrogen