title: Enhanced killing effect of nanosecond pulse electric fields on PANC1 and Jurkat cell lines in the presence of Tween 80.
abstract: We investigated the effects of nanosecond pulse electric fields (nsPEFs) on Jurkat and PANC1 cells, which are human carcinoma cell lines, in the presence of Tween 80 (T80) at a concentration of 0.18% and demonstarted an enhanced killing effect. We used two biological assays to determine cell viability after exposing cells to nsPEFs in the presence of T80 and observed a significant increase in the killing effect of nsPEFs. We did not see a toxic effect of T80 when cells were exposed to surfactant alone. However, we saw a synergistic effect when cells exposed to T80 were combined with the nsPEFs. Increasing the time of exposure for up to 8 h in T80 led to a significant decrease in cell viability when nsPEFs were applied to cells compared to control cells. We also observed cell type-specific swelling in the presence of T80. We suggest that T80 acts as an adjuvant in facilitating the effects of nsPEFs on the cell membrane; however, the limitations of the viability assays were addressed. We conclude that T80 may increase the fragility of the cell membrane, which makes it more susceptible to nsPEF-mediated killing.
authors: Frank Reidy Research Center for Bioelectrics, Old Dominion University, 4211 Monarch Way, STE-300, Norfolk, VA 23508, USA.
authors: Gaurav
authors: G
authors: Basu
authors: Bhargava Subhash
authors: BS
authors: Kalluri
authors: Ahmet Can
authors: AC
authors: Sabuncu
authors: Christopher J
authors: CJ
authors: Osgood
authors: Michael W
authors: MW
authors: Stacey
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects*; D004560:Electricity / Q000009:adverse effects*; D006801:Humans; D019169:Jurkat Cells; D011136:Polysorbates / Q000494:pharmacology*
chemical_list: D011136:Polysorbates