title: Recombinatorial biases and convergent recombination determine interindividual TCRβ sharing in murine thymocytes.
abstract: Overlap of TCR repertoires among individuals provides the molecular basis for public T cell responses. By deep-sequencing the TCRβ repertoires of CD4+CD8+ thymocytes from three individual mice, we observed that a substantial degree of TCRβ overlap, comprising ∼10-15% of all unique amino acid sequences and ∼5-10% of all unique nucleotide sequences across any two individuals, is already present at this early stage of T cell development. The majority of TCRβ sharing between individual thymocyte repertoires could be attributed to the process of convergent recombination, with additional contributions likely arising from recombinatorial biases; the role of selection during intrathymic development was negligible. These results indicate that the process of TCR gene recombination is the major determinant of clonotype sharing between individuals.
authors: State Key Laboratory of Cellular Stress Biology and School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China.
authors: Hanjie
authors: H
authors: Li
authors: Congting
authors: C
authors: Ye
authors: Guoli
authors: G
authors: Ji
authors: Xiaohui
authors: X
authors: Wu
authors: Zhe
authors: Z
authors: Xiang
authors: Yuanyue
authors: Y
authors: Li
authors: Yonghao
authors: Y
authors: Cao
authors: Xiaolong
authors: X
authors: Liu
authors: Daniel C
authors: DC
authors: Douek
authors: David A
authors: DA
authors: Price
authors: Jiahuai
authors: J
authors: Han
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology*; D005260:Female; D051379:Mice; D011897:Random Allocation; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000502:physiology*; D011995:Recombination, Genetic / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D060152:V(D)J Recombination / Q000276:immunology*
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta