title: Evaluation of the pharmacological function of ulinastatin in experimental animals.
abstract: Organ protection is a routine therapy in severe injuries. Our aim was to evaluate the beneficial effects of ulinastatin in experimental rats. Rats were randomly divided into a sham control, a model control and an ulinastatin-treated group. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined. Serum amylase, serum aspartate aminotransaminase (AST), lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CKMD) activities, interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), nitric oxide (NO) and cardiac troponin I (nTnl) levels were examined. Results showed that ulinastatin decreased MDA levels and ameliorated the down-regulation of SOD activity. In addition, ulinastatin pretreatment may decrease serum AST, LDH and CKMD activities, IL-8, TNF-α, and nTnl levels, and enhance NO level. Our results demonstrated that oxidative injury occurred after IR and that ulinastatin exhibits significant protective effects against these effects.
authors: Department of Cardiac Surgery, Provincial Hospital Affiliated to Shandong University, 324# Jingwu-Weiqi Road, Jinan, 250021, China.
authors: Chong-En
authors: CE
authors: Xu
authors: Meng-Yuan
authors: MY
authors: Zhang
authors: Cheng-Wei
authors: CW
authors: Zou
authors: Ling
authors: L
authors: Guo
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002316:Cardiotonic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003402:Creatine Kinase / Q000097:blood; D004353:Drug Evaluation, Preclinical; D006023:Glycoproteins / Q000494:pharmacology* / Q000627:therapeutic use; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D016209:Interleukin-8 / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000097:blood / Q000517:prevention & control*; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood; D019210:Troponin I / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D017725:Ventricular Pressure / Q000187:drug effects
chemical_list: D002316:Cardiotonic Agents; D006023:Glycoproteins; D016209:Interleukin-8; D019210:Troponin I; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase; C028665:urinastatin