title: [Gram-negative bacteria influence on the ability of the arginine-vasotocin to stimulate osmotic permeability of the frog urinary bladder epithelium].
abstract: The influence of endogenous gram-negative bacteria colonizing the mucosal epithelium of frog Rana temporaria L. urinary bladders (FUB) on arginine-vasotocin AVT-stimulated osmotic water flow in isolated urinary bladders was investigated. 170 animals were examined and only 40% were contaminated with gram-negative bacteria (about 10(3)-10(6) CFU per hemibladder). Several Enterobacteriaceae species were identified (Hafnia alvei, 36.7%, E. coli, 32.3%, Serratia marcescens, 8.8%, Citrobacter freundii, 4.4% etc.). Basal osmotic water flow level was invariable in "clean" and contaminated FUB, whereas bacterial contamination resulted in considerable decrease in AVT-stimulated water flow ("clean": 2.53 +/- 0.13, n = 59, contaminated: 1.21 +/- 0.17 me/min/cm2, n = 38, p < 0.001, within first 15 min of incubation with 5 x 10(-10)M AVT). Gentamycin protection assay revealed predominantly adhesive forms of bacteria. Thus our data indicated that the presence of gram-negative bacteria colonizing the mucosal epithelium of the urinary bladder results in decreased adility of ADH to rise osmotic water permeability which in turn could impair body osmoregulation.
authors: E A
authors: EA
authors: Lavrova
authors: L S
authors: LS
authors: Gretchenko
authors: R G
authors: RG
authors: Parnova
authors: E M
authors: EM
authors: Fok
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D058491:Bacterial Load; D001692:Biological Transport / Q000187:drug effects; D004755:Enterobacteriaceae / Q000502:physiology*; D005839:Gentamicins / Q000494:pharmacology; D008297:Male; D009924:Organ Culture Techniques; D009995:Osmosis; D010539:Permeability / Q000187:drug effects; D011896:Rana temporaria; D001743:Urinary Bladder / Q000187:drug effects* / Q000502:physiology; D019459:Urothelium / Q000187:drug effects* / Q000502:physiology; D014668:Vasotocin / Q000494:pharmacology*; D014867:Water / Q000378:metabolism*; D014882:Water-Electrolyte Balance / Q000187:drug effects* / Q000502:physiology
chemical_list: D005839:Gentamicins; D014867:Water; D014668:Vasotocin