title: Dynamic alteration of the colonic microbiota in intestinal ischemia-reperfusion injury.
abstract: BACKGROUND
  Intestinal ischemia-reperfusion (I/R) plays an important role in critical illnesses. Gut flora participate in the pathogenesis of the injury. This study is aimed at unraveling colonic microbiota alteration pattern and identifying specific bacterial species that differ significantly as well as observing colonic epithelium change in the same injury model during the reperfusion time course.
  
  
  RESULTS
  Denaturing gradient gel electrophoresis (DGGE) was used to monitor the colonic microbiota of control rats and experimental rats that underwent 0.5 hour ischemia and 1, 3, 6, 12, 24, and 72 hours following reperfusion respectively. The microbiota similarity, bacterial diversity and species that characterized the dysbiosis were estimated based on the DGGE profiles using a combination of statistical approaches. The interested bacterial species in the gel were cut and sequenced and were subsequently quantified and confirmed with real-time PCR. Meanwhile, the epithelial barrier was checked by microscopy and D-lactate analysis. Colonic flora changed early and differed significantly at 6 hours after reperfusion and then started to recover. The shifts were characterized by the increase of Escherichia coli and Prevotella oralis, and Lactobacilli proliferation together with epithelia healing.
  
  
  CONCLUSIONS
  This study shows for the first time that intestinal ischemia-reperfusion results in colonic flora dysbiosis that follows epithelia damage, and identifies the bacterial species that contribute most.
authors: Research Institute of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
authors: Fan
authors: F
authors: Wang
authors: Qiurong
authors: Q
authors: Li
authors: Chenyang
authors: C
authors: Wang
authors: Chun
authors: C
authors: Tang
authors: Jieshou
authors: J
authors: Li
mesh_terms: D000818:Animals; D044822:Biodiversity; D003106:Colon / Q000382:microbiology* / Q000473:pathology; D007413:Intestinal Mucosa / Q000382:microbiology; D019344:Lactic Acid / Q000097:blood; D008297:Male; D054892:Metagenome; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000145:classification / Q000382:microbiology* / Q000473:pathology; D013997:Time Factors
chemical_list: D019344:Lactic Acid