title: A rapid and simplified method for protein silver staining in polyacrylamide gels.
abstract: Silver staining is widely used to detect protein in polyacrylamide gels when high sensitivity is required. A simple and rapid protocol for silver staining of proteins following PAGE was developed in the present study. The number of steps was reduced compared to conventional protocol by combining fixing, rinsing, and soaking into a single impregnating step, thus achieving detection of proteins in 20 min. The present method is as sensitive as current protocols with the advantage of saving time and costs.
authors: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China.
authors: Lijing
authors: L
authors: Zhao
authors: Cong
authors: C
authors: Liu
authors: Yinpeng
authors: Y
authors: Sun
authors: Liping
authors: L
authors: Ban
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000191:economics / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity; D016622:Silver Staining / Q000191:economics / Q000379:methods*; D013997:Time Factors
chemical_list: D011506:Proteins