title: Evaluation of the VITEK 2 Gram-negative (GN) microbial identification test card: collaborative study.
abstract: A collaborative study was conducted to evaluate the performance of the VITEK 2 Gram-negative (GN) Identification card for use with the VITEK 2 automated microbial identification system. The GN test card is used in the identification of fermenting and nonfermenting Gram-negative bacilli, including the select agent organisms Brucella melitensis, Francisella tularensis, Burkholderia mallei, B. pseudomallei, and Yersinia pestis. The VITEK 2 GN card is based on 47 biochemical tests measuring carbon source utilization, inhibition and resistance, and enzymatic activities. A total of 20 laboratories representing government, industry, and private testing facilities throughout the United States participated. In this study, 720 Gram-negative inclusivity isolates were analyzed by the GN Identification method. Of the 720 well-characterized isolates, 707 were identified correctly, 0 were misidentified, 0 were unidentified, and 13 were not characterized as a Gram-negative organism. Additionally, 120 isolates exclusive of fermenting and nonfermenting Gram-negative bacilli were screened by Gram stain. A total of 117 isolates were correctly excluded. Three organisms were incorrectly characterized by Gram stain procedures, resulting in incorrect analysis and misidentification by VITEK 2 GN. The VITEK 2 GN identification method is an acceptable automated method for the rapid identification of Gram-negative bacteria.
authors: Q Laboratories, Inc., Cincinnati, OH 45214, USA.
authors: Erin
authors: E
authors: Crowley
authors: Patrick
authors: P
authors: Bird
authors: Kiel
authors: K
authors: Fisher
authors: Katherine
authors: K
authors: Goetz
authors: Megan
authors: M
authors: Boyle
authors: M Joseph
authors: MJ
authors: Benzinger
authors: Marc
authors: M
authors: Juenger
authors: James
authors: J
authors: Agin
authors: David
authors: D
authors: Goins
authors: Ronald
authors: R
authors: Johnson
mesh_terms: D003299:Cooperative Behavior; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*