title: Schistosoma japonicum UDP-glucose 4-epimerase protein is located on the tegument and induces moderate protection against challenge infection.
abstract: Schistosomiasis is an important global public health problem, as millions of people are at risk of acquiring this infection. An ideal method for sustainable control of schistosomiasis is using a vaccine alone or in combination with drugs. In the present study, we cloned the SjGALE gene and generated the expression product in E. coli. The expression level of SjGALE during different developmental stages of S. japonicum was evaluated by real-time RT-PCR and western blotting. Immunolocalization indicated that the protein was mainly located on the tegument of the parasite. Infection of rSjGALE-immunized mice demonstrated a 34% and 49% reduction of the mean worm burden and liver egg burden, respectively, in two independent experiments, indicating immune protection. The liver egg count from each female adult worm was significantly reduced by 63% in the two trials. The cytokine profile and IgG isotype analysis demonstrated the induction of a Th1 immune profile in response to immunization with this protein, further suggesting protection against infection. In conclusion, these findings indicated that SjGALE is a potential vaccine against S. japonicum.
authors: National Laboratory of Animal Schistosomiasis Control, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, People's Republic of China.
authors: Pingping
authors: P
authors: Liu
authors: Yanli
authors: Y
authors: Shi
authors: Yunxia
authors: Y
authors: Yang
authors: Yufan
authors: Y
authors: Cao
authors: Yaojun
authors: Y
authors: Shi
authors: Hao
authors: H
authors: Li
authors: Jinming
authors: J
authors: Liu
authors: Jiaojiao
authors: J
authors: Lin
authors: Yamei
authors: Y
authors: Jin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D005620:Freund's Adjuvant / Q000276:immunology; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007114:Immunization; D008018:Life Cycle Stages; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D021381:Protein Transport; D012549:Schistosoma japonicum / Q000033:anatomy & histology* / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity; D012554:Schistosomiasis japonica / Q000276:immunology*; D014158:Transcription, Genetic; D014534:UDPglucose 4-Epimerase / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000909:Antibodies, Helminth; D018076:DNA, Complementary; D005620:Freund's Adjuvant; D014534:UDPglucose 4-Epimerase