title: Structure of a peptidoglycan-related polysaccharide from Providencia alcalifaciens O45.
abstract: A polysaccharide was isolated from the opportunistic human pathogen Providencia alcalifaciens O45:H26 by extraction with aqueous phenol and studied by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional ROESY and H-detected (1)H,(13)C HSQC experiments. The polysaccharide contains N-acetylglucosamine and N-acetylmuramic acid (D-GlcpNAc3Rlac) amidated with L-alanine and has the following structure: →4)-β-D-GlcpNAc-(1→4)-β-D-GlcpNAc3(Rlac-L-Ala)-(1→. The polysaccharide possesses a remarkable structural similarity to the bacterial cell wall peptidoglycan. It is not unique to the strain studied but is common to strains of at least four P. alcalifaciens O-serogroups (O3, O24, O38, and O45). No evidence was obtained that the polysaccharide is associated with the LPS, and hence it might represent a bacterial capsule component.
authors: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991 Moscow, Russia. olga.ovchinnikova@gmail.com
authors: O G
authors: OG
authors: Ovchinnikova
authors: B
authors: B
authors: Liu
authors: N A
authors: NA
authors: Kocharova
authors: A S
authors: AS
authors: Shashkov
authors: A N
authors: AN
authors: Kondakova
authors: M
authors: M
authors: Siwinska
authors: L
authors: L
authors: Feng
authors: A
authors: A
authors: Rozalski
authors: L
authors: L
authors: Wang
authors: Y A
authors: YA
authors: Knirel
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis; D000409:Alanine / Q000032:analysis; D016667:Bacterial Capsules / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009112:Muramic Acids / Q000032:analysis; D019081:O Antigens / Q000737:chemistry*; D010457:Peptidoglycan / Q000737:chemistry* / Q000302:isolation & purification; D011532:Providencia / Q000276:immunology*
chemical_list: D009112:Muramic Acids; D019081:O Antigens; D010457:Peptidoglycan; C031651:N-acetylmuramic acid; D000409:Alanine; D000117:Acetylglucosamine