title: Mast cell degranulation mediates compound 48/80-induced hyperalgesia in mice.
abstract: Mast cells mediate allergies, hypersensitivities, host defense, and venom neutralization. An area of recent interest is the contribution of mast cells to inflammatory pain. Here we found that specific, local activation of mast cells produced plantar hyperalgesia in mice. Basic secretagogue compound 48/80 induced plantar mast cell degranulation accompanied by thermal hyperalgesia, tissue edema, and neutrophil influx in the hindpaws of ND4 Swiss mice. Blocking mast cell degranulation, neutrophil extravasation, and histamine signaling abrogated these responses. Compound 48/80 also produced edema, pain, and neutrophil influx in WT C57BL/6 but not in genetically mast cell-deficient C57BL/6-Kit(W-sh)(/)(W-sh) mice. These responses were restored following plantar reconstitution with bone marrow-derived cultured mast cells.
authors: Department of Biology, Macalester College, St. Paul, MN 55105, USA. chatterjead@macalester.edu
authors: Devavani
authors: D
authors: Chatterjea
authors: Abigail
authors: A
authors: Wetzel
authors: Madison
authors: M
authors: Mack
authors: Camilla
authors: C
authors: Engblom
authors: Juliann
authors: J
authors: Allen
authors: Carolina
authors: C
authors: Mora-Solano
authors: Luisa
authors: L
authors: Paredes
authors: Evelyn
authors: E
authors: Balsells
authors: Tijana
authors: T
authors: Martinov
mesh_terms: D000818:Animals; D015550:Cell Degranulation; D004487:Edema / Q000139:chemically induced / Q000276:immunology; D006358:Hot Temperature; D006930:Hyperalgesia / Q000139:chemically induced / Q000276:immunology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D020556:Neutrophil Infiltration / Q000187:drug effects / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D003189:p-Methoxy-N-methylphenethylamine