title: A focused scanning vertical beam for charged particle irradiation of living cells with single counted particles.
abstract: The Surrey vertical beam is a new facility for targeted irradiation of cells in medium with singly counted ions. A duo-plasmatron ion source and a 2 MV Tandem™ accelerator supply a range of ions from protons to calcium for this beamline and microscope endstation, with energy ranges from 0.5 to 12 MeV. A magnetic quadrupole triplet lens is used to focus the beam of ions. We present the design of this beamline, and early results showing the capability to count single ions with 98% certainty on CR-39 track etch. We also show that the beam targeting accuracy is within 5 μm and selectively target human fibroblasts with a <5 μm carbon beam, using γ-H2AX immunofluorescence to demonstrate which cell nuclei were irradiated. We discuss future commissioning steps necessary to achieve submicron targeting accuracy with this beamline.
authors: Ion Beam Centre, University of Surrey, Guildford, Surrey GU2 7XH, United Kingdom. m.merchant@surrey.ac.uk
authors: Michael J
authors: MJ
authors: Merchant
authors: Jonathan C G
authors: JC
authors: Jeynes
authors: Geoffrey W
authors: GW
authors: Grime
authors: Vladimir
authors: V
authors: Palitsin
authors: Iain D W
authors: ID
authors: Tullis
authors: Paul R
authors: PR
authors: Barber
authors: Boris
authors: B
authors: Vojnovic
authors: Roger P
authors: RP
authors: Webb
authors: Karen J
authors: KJ
authors: Kirkby
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D005246:Feedback; D006801:Humans; D006813:Humidity; D036103:Nanotechnology / Q000295:instrumentation*; D010315:Particle Accelerators / Q000295:instrumentation*; D011853:Radiobiology / Q000295:instrumentation*; D013696:Temperature