title: Studies on the dissociation and urea-induced unfolding of FtsZ support the dimer nucleus polymerization mechanism.
abstract: FtsZ is a major protein in bacterial cytokinesis that polymerizes into single filaments. A dimer has been proposed to be the nucleating species in FtsZ polymerization. To investigate the influence of the self-assembly of FtsZ on its unfolding pathway, we characterized its oligomerization and unfolding thermodynamics. We studied the assembly using size-exclusion chromatography and fluorescence spectroscopy, and the unfolding using circular dichroism and two-photon fluorescence correlation spectroscopy. The chromatographic analysis demonstrated the presence of monomers, dimers, and tetramers with populations dependent on protein concentration. Dilution experiments using fluorescent conjugates revealed dimer-to-monomer and tetramer-to-dimer dissociation constants in the micromolar range. Measurements of fluorescence lifetimes and rotational correlation times of the conjugates supported the presence of tetramers at high protein concentrations and monomers at low protein concentrations. The unfolding study demonstrated that the three-state unfolding of FtsZ was due to the mainly dimeric state of the protein, and that the monomer unfolds through a two-state mechanism. The monomer-to-dimer equilibrium characterized here (K(d) = 9 μM) indicates a significant fraction (~10%) of stable dimers at the critical concentration for polymerization, supporting a role of the dimeric species in the first steps of FtsZ polymerization.
authors: Laboratorio de Biología Estructural y Molecular, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
authors: Felipe
authors: F
authors: Montecinos-Franjola
authors: Justin A
authors: JA
authors: Ross
authors: Susana A
authors: SA
authors: Sánchez
authors: Juan E
authors: JE
authors: Brunet
authors: Rosalba
authors: R
authors: Lagos
authors: David M
authors: DM
authors: Jameson
authors: Octavio
authors: O
authors: Monasterio
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000648:ultrastructure*; D019281:Dimerization; D008956:Models, Chemical; D008958:Models, Molecular; D011108:Polymers / Q000737:chemistry; D011489:Protein Denaturation; D017510:Protein Folding; D014508:Urea / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; C071525:FtsZ protein, Bacteria; D011108:Polymers; D014508:Urea