title: Cryopreserved reticulocytes derived from hematopoietic stem cells can be invaded by cryopreserved Plasmodium vivax isolates.
abstract: The development of a system for the continuous culture of Plasmodium vivax in vitro would benefit from the use of reticulocytes derived from differentiated hematopoietic stem cells (HCS). At present, the need to use both fresh reticulocytes and fresh P. vivax isolates represents a major obstacle towards this goal, particularly for laboratories located in non-endemic countries. Here, we describe a new method for the cryopreservation of HSC-derived reticulocytes to be used for both P. falciparum and P. vivax invasion tests. Cryopreserved P. falciparum and P. vivax isolates could invade both fresh and cryopreserved HSC-derived reticulocytes with similar efficiency. This new technique allows the storage of HSC-derived reticulocytes which can be used for later invasion tests and represents an important step towards the establishment of a continuous P. vivax culture.
authors: Unit of Malariology, Institute of Tropical Medicine Antwerp, Belgium. fnoulin@itg.be
authors: Florian
authors: F
authors: Noulin
authors: Céline
authors: C
authors: Borlon
authors: Peter
authors: P
authors: van den Eede
authors: Luc
authors: L
authors: Boel
authors: Catherine M
authors: CM
authors: Verfaillie
authors: Umberto
authors: U
authors: D'Alessandro
authors: Annette
authors: A
authors: Erhart
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation; D015925:Cryopreservation; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D010966:Plasmodium vivax / Q000302:isolation & purification* / Q000502:physiology*; D011247:Pregnancy; D012156:Reticulocytes / Q000166:cytology* / Q000469:parasitology*