title: Novel single  nucleotide polymorphisms of the bovine methyltransferase 3b gene and their  association with meat quality traits in beef cattle.
abstract: DNA  methylation is essential for adipose deposition in mammals. We screened SNPs of  the bovine DNA methyltransferase 3b (DNMT3b) gene in Snow Dragon beef, a  commercial beef cattle population in China. Nine SNPs were found in the  population and three of six novel SNPs were chosen for genotyping and analyzing  a possible association with 16 meat quality traits. The frequencies of the  alleles and genotypes of the three SNPs in Snow Dragon beef were similar to  those in their terminal-paternal breed, Wagyu. Association analysis disclosed  that SNP1 was not associated with any of the traits; SNP2 was significantly  associated with lean meat color score and chuck short rib score, and SNP3 had a  significant effect on dressing percentage and back-fat thickness in the beef  population. The individuals with genotype GG for SNP2 had a 25.7% increase in  lean meat color score and a 146% increase in chuck short rib score, compared  with genotype AA. The cattle with genotype AG for SNP3 had 35.7 and 24%  increases in dressing percentage and 28.8 and 29.2% increases in back-fat  thickness, compared with genotypes GG and AA, respectively. Genotypic  combination analysis revealed significant interactions between SNP1 and SNP2  and between SNP2 and SNP3 for the traits rib-eye area and live weight. We  conclude that there is considerable evidence that DNMT3b is a determiner  of beef quality traits.
authors: Key Laboratory of Agricultural Animal and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, P.R. China.
authors: X
authors: X
authors: Liu
authors: X Y
authors: XY
authors: Guo
authors: X Z
authors: XZ
authors: Xu
authors: M
authors: M
authors: Wu
authors: X
authors: X
authors: Zhang
authors: Q
authors: Q
authors: Li
authors: P P
authors: PP
authors: Ma
authors: Y
authors: Y
authors: Zhang
authors: C Y
authors: CY
authors: Wang
authors: F J
authors: FJ
authors: Geng
authors: C H
authors: CH
authors: Qin
authors: L
authors: L
authors: Liu
authors: W H
authors: WH
authors: Shi
authors: Y C
authors: YC
authors: Wang
authors: Y
authors: Y
authors: Yu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D005838:Genotype; D008460:Meat; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D019655:Quantitative Trait, Heritable; D000097584:DNA Methyltransferase 3B
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases; D000097584:DNA Methyltransferase 3B