title: Dengue virus infection-enhancing and neutralizing antibody balance in children of the Philippines and Indonesia.
abstract: Dengue fever and dengue hemorrhagic fever are important diseases worldwide. Although antibody-dependent enhancement of infection has been proposed as a mechanism for increased disease severity, enhancing antibodies in endemic people have not been thoroughly investigated. Recently, we established a serological assay system to measure the balance of enhancing and neutralizing activities, which provides useful information for estimating in vivo antibody status. We measured the balance of these activities against four dengue virus (DENV) types in endemic populations, and analyzed the proportion of sera containing enhancing and neutralizing antibodies. Predominantly healthy Filipino children were used for analysis, although a population of Indonesian children was also investigated. In the Filipino population, the highest proportion of neutralizing activities was shown against DENV2, followed by DENV1. A greater proportion of sera exhibited enhancing rather than neutralizing antibodies against other virus types. Neutralizing activities were complement-dependent, while enhancing activities were complement-independent. The Indonesian population showed a similar dengue antibody status. Our results indicate that a relatively high proportion of endemic children possessed complement-independent enhancing antibodies against some DENV types.
authors: Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Airlangga University, Kampus C, UNAIR Jl. Mulyorejo, Surabaya, Indonesia. paradios99@yahoo.co.jp
authors: Atsushi
authors: A
authors: Yamanaka
authors: Yuko
authors: Y
authors: Tabuchi
authors: Kris C
authors: KC
authors: Mulyatno
authors: Helen
authors: H
authors: Susilowati
authors: Eryk
authors: E
authors: Hendrianto
authors: Soegeng
authors: S
authors: Soegijanto
authors: Eiji
authors: E
authors: Konishi
mesh_terms: D019138:Antibodies, Blocking / Q000097:blood / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D019067:Antibody-Dependent Enhancement / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003165:Complement System Proteins / Q000276:immunology; D003715:Dengue / Q000453:epidemiology / Q000276:immunology* / Q000821:virology; D003716:Dengue Virus / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D019353:Endemic Diseases; D006801:Humans; D007214:Indonesia / Q000453:epidemiology; D007223:Infant; D009500:Neutralization Tests; D010679:Philippines / Q000453:epidemiology; D011951:Receptors, Complement / Q000276:immunology; D019595:Severe Dengue / Q000453:epidemiology / Q000276:immunology / Q000821:virology
chemical_list: D019138:Antibodies, Blocking; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D011951:Receptors, Complement; D003165:Complement System Proteins