title: [Enzymatic extraction and antibacterial activity of aucubin from Eucommia ulmoides leaves].
abstract: OBJECTIVE
  To investigate the technology of Aucubin in Eucommia ulmoides leaves extracted by enzymatic method and its antibacterial activity.
  
  
  METHODS
  Aucubin in Eucommia ulmoides leaves was extracted by cellulase method. The extraction technology was optimized using the content of Aucubin as index. The antibacterial activity of Aucubin was determined.
  
  
  RESULTS
  The results showed that the optimum technology was as follows; The solid-liquid ratio was 1:12, extracted for 50 min by 0.4% enzyme at 50 degrees C in pH 6.0. The extraction rate of Aucubin was as high as 17.892 mg/g. The Aucubin extracted could obviously inhibit the growth of Escherichia coli and Staphylococcus aureus, the MIC of Aucubin against Staphylococcus aureus and Escherichia coli were 4.832 mg/mL and 9.664 mg/mL respectively. However, Aucubin presented weak inhibitory effect on Streptococcus pneumonia and MG-hemolytic streptococcus, the MIC of Aucubin against them were all 28.946 mg/mL.
  
  
  CONCLUSIONS
  The extraction technology obtained in this experiment is reasonable and feasible with high extraction rate, and the Aucubin has some antibacterial activity.
authors: Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China. zhengjie666@gmail.com
authors: Jie
authors: J
authors: Zheng
authors: Duan
authors: D
authors: Liu
authors: Su-qing
authors: SQ
authors: Zhao
authors: Juan
authors: J
authors: Su
authors: Qiu-ping
authors: QP
authors: Yan
authors: Lei
authors: L
authors: Chen
authors: Yi
authors: Y
authors: Xiao
authors: Chun-mei
authors: CM
authors: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002480:Cellulase / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects; D031283:Eucommiaceae / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D057888:Iridoid Glucosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D057888:Iridoid Glucosides; C006650:aucubin; D002480:Cellulase