title: Influence of S. aureus exosecretions on cytokine profile in bovine leukocyte cultures in vitro.
abstract: The aim of the research was to evaluate the in vitro effect of Staphylococcus aureus exosecretions on the expression of genes encoding IL-2 and IL-12 and secretion of IFN-γ and TNF-α, in bovine leukocyte cultures in vitro. The research was based on 30 S. aureus isolates collected from milk samples from cows with clinical mastitis. Supernatants prepared from the bacterial liquid cultures, which were used to treat leukocytes, were divided into three groups: one with superantigen-like properties, one with leukotoxic-like properties and the one without superantigen or leukotoxic-like properties. The MNC, PMN and MIX (consisted of MNC and PMN leukocytes) cultures were grown and treated with the supernatants. The work shows that the effect on the cytokine gene expression and cytokine secretion caused by S. aureus exosecretions is mainly due to the presence of virulence factors connected with superantigen-like activity and less with leukotoxic-like activity whereas exosecretions of other activity are not or only slightly involved in this process.
authors: Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Doktora Judyma 24, 71-466 Szczecin, Poland. karol.fijalkowski@zut.edu.pl
authors: Karol
authors: K
authors: Fijałkowski
authors: Danuta
authors: D
authors: Czernomysy-Furowicz
authors: Paweł
authors: P
authors: Nawrotek
authors: Jolanta
authors: J
authors: Karakulska
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism*
chemical_list: D003470:Culture Media; D016207:Cytokines