title: Construction and hemocompatibility study of highly bioactive heparin-functionalized surface.
abstract: A simple method is developed to construct anticoagulant surfaces via passive adsorption of heparin onto the protonated plasma-polymerized allylamine (PPAam) films from phosphate-buffered saline (PBS). These protonated PPAam surfaces are found to have high affinity to heparin. Importantly, the heparin-functionalized PPAam (Hep-PPAam) surfaces show good retention of heparin after long-term immersion in PBS. The Hep-PPAam surface prolongs the activated partial thromboplastin time for about 20 s as compared to 316L stainless steel even though the adsorption amount of heparin is only about 300 ng/cm(2). This indicates that the heparin bound to the protonated PPAam surfaces in this way maintains a high bioactivity. Blood platelet adhesion and activation on this surface is remarkably reduced and adsorption and activation of fibrinogen is inhibited. Thus, Hep-PPAam surface modification leads to a significant improve of the hemocompatibility.
authors: Key Laboratory of Advanced Technology for Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China.
authors: Zhi-Lu
authors: ZL
authors: Yang
authors: Shuo
authors: S
authors: Zhou
authors: Lei
authors: L
authors: Lu
authors: Xin
authors: X
authors: Wang
authors: Jin
authors: J
authors: Wang
authors: Nan
authors: N
authors: Huang
mesh_terms: D000327:Adsorption; D000925:Anticoagulants / Q000737:chemistry / Q000494:pharmacology*; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D005340:Fibrinogen / Q000378:metabolism; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D010314:Partial Thromboplastin Time; D010973:Platelet Adhesiveness / Q000187:drug effects; D011073:Polyamines / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000925:Anticoagulants; D020099:Coated Materials, Biocompatible; D011073:Polyamines; C063994:polyallylamine; D005340:Fibrinogen; D006493:Heparin