title: Rapid and sensitive detection of auxins and flavonoids in plant samples by high-performance liquid chromatography coupled with tandem mass spectrometry.
abstract: Simultaneous determination of indole-3-acetic acid and methyl indole-3-acetic acid ester in small amounts of plant tissue is essential for elucidating their mutual transformation mechanism and the in vivo function of methyl indole-3-acetic acid ester. Rapid quantification of flavonoids in the same sample is important for clarifying their roles in the transport of auxins and other phytohormones. Herein, we describe a simple method for the simultaneous determination of indole-3-acetic acid and its methyl ester in the roots of the Arabidopsis thaliana seedlings and a protocol for the rapid extraction and quantification of quercetin and kaempferol in these seedlings. High-performance liquid chromatography coupled with electrospray ionization time-of-flight tandem mass spectrometry was used for the detection of all the compounds. Negative data for indole-3-acetic acid and positive data for methyl indole-3-acetic acid ester were collected in two successive files with a single injection of the extracted sample. Under optimized conditions, the limit of detection for the four compounds was 2 ng/mL for indole-3-acetic acid, 0.5 ng/mL for methyl indole-3-acetic acid ester, 5 ng/mL for quercetin, and 1 ng/mL for kaempferol, respectively. Because of the high sensitivity of the assay, only 2-10 mg of the plant material was required to obtain quantitative results.
authors: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
authors: Yuan
authors: Y
authors: Liang
authors: Xiaocui
authors: X
authors: Zhu
authors: Tongbo
authors: T
authors: Wu
authors: Meiping
authors: M
authors: Zhao
authors: Huwei
authors: H
authors: Liu
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007210:Indoleacetic Acids / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D010937:Plant Growth Regulators / Q000032:analysis; D011794:Quercetin / Q000032:analysis*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007210:Indoleacetic Acids; D010936:Plant Extracts; D010937:Plant Growth Regulators; D011794:Quercetin