title: Transducing methyltransferase activity into electrical signals in a carbon nanotube-DNA device().
abstract: This study creates a device where the DNA is electronically integrated to serve as both the biological target and electrical transducer in a CNT-DNA-CNT device. We detect DNA binding and methylation by the methyltransferase M.SssI at the single molecule level. We demonstrate sequence-specific, reversible binding of M.SssI and protein-catalyzed methylation that alters the protein-binding affinity of the device. This device, which relies on the exquisite electrical sensitivity of DNA, represents a unique route for the specific, single molecule detection of enzymatic activity.
authors: Department of Chemistry, Columbia University, New York, NY, 10027, USA;
authors: Hanfei
authors: H
authors: Wang
authors: Natalie B
authors: NB
authors: Muren
authors: David
authors: D
authors: Ordinario
authors: Alon A
authors: AA
authors: Gorodetsky
authors: Jacqueline K
authors: JK
authors: Barton
authors: Colin
authors: C
authors: Nuckolls