title: Toxins from Physalia physalis (Cnidaria) raise the intracellular Ca(2+) of beta-cells and promote insulin secretion.
abstract: Physalia physalis is a marine cnidarian from which high molecular weight toxins with hemolytic and neurotoxic effects have been isolated. In the present work, two novel toxins, PpV9.4 and PpV19.3 were purified from P. physalis by bioactive guideline isolation. It involved two steps of column chromatography, gel filtration and RP-HPLC. The molecular weights were 550.7 and 4720.9 Da for PpV9.4 and PpV19.3, respectively. In the light of the Edman sequencing results, the structure of these toxins included the presence of modified amino acids. Both toxins increased the percentage of insulin secreting beta-cells and induced cytosolic Ca2+ elevation. To date, this is the first report of low molecular weight toxins increasing insulin secretion purified from cnidarians, by constituting a new approach to the study of beta-cells physiology.
authors: Department of Neural Development and Physiology, Instituto de Fisiologia Celular, Universidad Nacional Autonoma de Mexico/Circuito Ext. SN, UNAM, Coyoacan, Mexico DF. CP 04510, Mexico.
authors: C M
authors: CM
authors: Diaz-Garcia
authors: D
authors: D
authors: Fuentes-Silva
authors: C
authors: C
authors: Sanchez-Soto
authors: D
authors: D
authors: Domínguez-Pérez
authors: N
authors: N
authors: García-Delgado
authors: C
authors: C
authors: Varela
authors: G
authors: G
authors: Mendoza-Hernández
authors: A
authors: A
authors: Rodriguez-Romero
authors: O
authors: O
authors: Castaneda
authors: M
authors: M
authors: Hiriart
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D056148:Chromatography, Reverse-Phase; D006461:Hemolysis / Q000187:drug effects; D037402:Hydrozoa / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014118:Toxins, Biological / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D007328:Insulin; D014118:Toxins, Biological; D002118:Calcium