title: AFM investigation on Ox-LDL-induced changes in cell spreading and cell-surface adhesion property of endothelial cells.
abstract: The integrity and adhesion properties of endothelium play vital roles during atherosclerosis. It is well known that oxidized low-density lipoprotein (Ox-LDL) influences many physiological activities or mechanical properties of endothelial cells. However, the effects of Ox-LDL on the integrity and nonspecific adhesion properties of endothelial cells are still unclear. In this study, using the topographical imaging and force measurement functions of atomic force microscopy (AFM), we found that Ox-LDL can transiently weaken the integrity of endothelium by impairing cell spreading of endothelial cells and decrease the attachment of irrelevant blood cells to endothelium by impairing the nonspecific adhesion property of endothelial cells. The AFM-based data provide important information for understanding the effects of Ox-LDL on endothelial cells or during atherogenesis.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.
authors: Wenxiang
authors: W
authors: Shao
authors: Hua
authors: H
authors: Jin
authors: Jie
authors: J
authors: Huang
authors: Bin
authors: B
authors: Qiu
authors: Ruihua
authors: R
authors: Xia
authors: Zeyuan
authors: Z
authors: Deng
authors: Jiye
authors: J
authors: Cai
authors: Yong
authors: Y
authors: Chen
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein