title: Structural and functional characterization of the bacterial translocation inhibitor GE82832.
abstract: The structure of GE82832, a translocation inhibitor produced by a soil microorganism, is shown to be highly related to that of dityromycin, a bicyclodecadepsipeptide antibiotic discovered long ago whose characterization had never been pursued beyond its structural elucidation. GE82832 and dityromycin were shown to interfere with both aminoacyl-tRNA and mRNA movement and with the Pi release occurring after ribosome- and EF-G-dependent GTP hydrolysis. These findings and the unusual ribosomal localization of GE82832/dityromycin near protein S13 suggest that the mechanism of inhibition entails an interference with the rotation of the 30S subunit "head" which accompanies the ribosome-unlocking step of translocation.
authors: Department of Biosciences & Biotechnology, University of Camerino, Camerino (MC), Italy.
authors: Letizia
authors: L
authors: Brandi
authors: Sonia
authors: S
authors: Maffioli
authors: Stefano
authors: S
authors: Donadio
authors: Fabio
authors: F
authors: Quaglia
authors: Marco
authors: M
authors: Sette
authors: Pohl
authors: P
authors: Milón
authors: Claudio O
authors: CO
authors: Gualerzi
authors: Attilio
authors: A
authors: Fabbretti
mesh_terms: D000192:Actinomycetales / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D018988:Bacterial Translocation / Q000187:drug effects; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D008958:Models, Molecular; D015394:Molecular Structure; D020653:Peptide Elongation Factor G / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D012988:Soil Microbiology; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C512383:GE82832 peptide, Streptosporangium cinnabarinum; D020653:Peptide Elongation Factor G; D010455:Peptides; D006160:Guanosine Triphosphate