title: Structure of solid-supported lipid membrane probed by noble metal nanoparticle deposition.
abstract: Direct deposition of a noble metal layer onto a solid-supported membrane was proposed as an indirect microscopy tool to visually observe different lipid phases that may develop in the lipid membrane. The method relied on the different permeability of the lipid membrane towards the incident atoms during deposition. Liquid state or structural defects such as phase boundaries, step ledges in a multi-lamellar stack, and pores permitted the metal atoms to penetrate and nucleate inside the membrane whereas rigid gel state was relatively impermeable to the incident atoms, thus enabling visualization of liquid phase or structural defects inside the gel state. Based on the proposed method, we demonstrated the phase states resulting from thermotropic transitions of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), dioleoylphosphatidylethanolamine (DOPE)/DPPC mixture, and 1,2-dioleoyl-3-trimethylammonium propane (DOTAP). Although the proposed method does not allow in-situ observation of equilibrium states, the method should be an excellent complementary tool for visualizing the lipid phases as the method can resolve fine structural details (up to tens of nanometer scale) as seen in the DPPC membrane while providing macroscopic images (up to several micrometers).
authors: Hanyang University, Seoul, Republic of Korea.
authors: Hyeun Hwan
authors: HH
authors: An
authors: Seung Jae
authors: SJ
authors: Lee
authors: Hee-Soo
authors: HS
authors: Kim
authors: Won Bae
authors: WB
authors: Han
authors: Chong Seung
authors: CS
authors: Yoon
mesh_terms: D008563:Membrane Lipids / Q000737:chemistry*; D053768:Metal Nanoparticles; D008670:Metals / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D010743:Phospholipids / Q000737:chemistry*
chemical_list: D008563:Membrane Lipids; D008670:Metals; D010743:Phospholipids