title: HILIC-MS/MS method for the quantitation of nucleotides in infant formula and adult nutritional formula: First Action 2011.21.
abstract: Official Method 2011.21 is for the quantitation of the following nucleotides: adenosine 5'-monophosphate (AMP), guanosine 5'-monophosphate (GMP), uridine 5'-monophosphate (UMP), cytidine 5'-monophosphate (CMP), and inosine 5'-monophosphate (IMP) in infant formula and adult/pediatric nutritional formula. It uses hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC-MS/MS). Preparation of the internal standards was conducted using centrifugal ultrafiltration and the standards are AMP- (13)C10, (15)N5; GMP-(13)C10, (15)N5; UMP-(13)C9, (15)N2; and15 CMP- (13)C9, (15)N3. Data were collected by using multiple reaction monitoring of the product ions of protonated molecules of the five nucleotides generated by positive-electrospray ionization. The HILIC conditions were conducted with ammonium formate (30 mmol/L) in water (pH 2.5, adjusted with formic acid) and methanol. The LOD and LOQ of the standard solution were 0.005-0.01 and 0.01-0.03 microg/mL, respectively. Recovery data were collected for intraday and interday testing and ranged from 98.1 to 108.9% with an RSD of 0.7-5.4%. The analytical range of the method is between 0.04 to 5 microg/mL for standard solution.
authors: Kinjo Gakuin University, School of Pharmacy, Department of Physical and Analytical Chemistry, Nagoya, Japan. kinoue@u-shizuoka-ken.ac.jp
authors: Koichi
authors: K
authors: Inoue
authors: Dawn
authors: D
authors: Dowell
mesh_terms: D000249:Adenosine Monophosphate / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D003568:Cytidine Monophosphate / Q000032:analysis; D006157:Guanosine Monophosphate / Q000032:analysis; D041943:Infant Formula / Q000737:chemistry*; D007291:Inosine Monophosphate / Q000032:analysis; D011685:Purine Nucleotides / Q000032:analysis*; D011742:Pyrimidine Nucleotides / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014542:Uridine Monophosphate / Q000032:analysis
chemical_list: D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D007291:Inosine Monophosphate; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D014542:Uridine Monophosphate; D003568:Cytidine Monophosphate