title: Coagulation profile of liquid-state plasma.
abstract: BACKGROUND
  Use of liquid plasma (LP) has been reported as early as the mid 1930s. Unlike fresh-frozen plasma (FFP), LP is maintained at 1 to 6°C for up to 40 days after collection and processing. Despite its approved use by the US Food and Drug Administration, the coagulation profile of LP is incompletely described. In this study we evaluate the coagulation profile of LP stored up to 30 days.
  
  
  METHODS
  LP was prepared by removing plasma from nonleukoreduced whole blood within 24 hours of collection. Three LP units from each ABO group were collected and stored at 1 to 6°C. Plasma aliquots were obtained at Postcollection Days 1 to 5, 10, 15, 20, 25, and 30 and then stored at -70°C. Each aliquot was tested for prothrombin time, activated partial thromboplastin time, and other coagulation and fibrinolytic factors.
  
  
  RESULTS
  There was a significant decrease in Factor (F)V, FVII, FVIII, von Willebrand factor (VWF), protein S (PS) activity, and endogenous thrombin potential on Day 15 compared with Day 1. No significant difference was observed for PS antigen, D-dimer, or thrombin-antithrombin complex. At least 50% activity of all measured factors was noted on Day 15, compared to Day 1. Considerable heterogeneity was observed between the different blood groups for FVII, FVIII, and VWF.
  
  
  CONCLUSIONS
  These data demonstrate that LP maintains at least 50% of factor activity and thrombin-generating capacity up to 15 days of refrigerated storage. It may be more appropriate to limit LP storage and supplement with FFP when used for management of massively bleeding patients.
authors: Department of Medical Pathology and Laboratory Medicine, the Trauma Division, University of California, Davis Health System, Sacramento, California 95817, USA.
authors: Robert C
authors: RC
authors: Gosselin
authors: Carol
authors: C
authors: Marshall
authors: Denis M
authors: DM
authors: Dwyre
authors: Chris
authors: C
authors: Gresens
authors: Diana
authors: D
authors: Davis
authors: Lynette
authors: L
authors: Scherer
authors: Douglas
authors: D
authors: Taylor
mesh_terms: D000328:Adult; D001777:Blood Coagulation / Q000502:physiology*; D001779:Blood Coagulation Factors / Q000032:analysis / Q000378:metabolism; D001780:Blood Coagulation Tests; D001793:Blood Preservation / Q000379:methods; D005343:Fibrinolytic Agents / Q000032:analysis; D006487:Hemostasis / Q000502:physiology; D006801:Humans; D008297:Male; D010314:Partial Thromboplastin Time; D010949:Plasma / Q000737:chemistry / Q000502:physiology*; D059748:Proteolysis; D011517:Prothrombin Time / Q000379:methods
chemical_list: D001779:Blood Coagulation Factors; D005343:Fibrinolytic Agents