title: Novel lipoidal amine-based nanocarrier formulations for siRNA delivery.
abstract: BACKGROUND
  Lack of safe and efficient delivery of siRNA remains the greatest hurdle for the therapeutic application of siRNA. This article reports synthesis and evaluation of novel lipoidal amine-based nanocarrier (LANC) formulations for siRNA delivery.
  
  
  METHODS
  Physicochemical properties were analyzed for LANC formulations. siRNA delivery efficiency of LANC-siRNA complexes was determined using a luciferase reporter gene assay. Cytotoxicity of the LANC-siRNA complexes was measured by the MTS assay. Finally, cellular uptake and cytoplasmic release of siRNA were analyzed using flow cytometry.
  
  
  CONCLUSIONS
  The LANC formulation facilitated siRNA uptake and release into the cytoplasm, mediating significant luciferase knockdown (70% inhibition).
authors: Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
authors: Chenguang
authors: C
authors: Zhou
authors: Zhang
authors: Z
authors: Yue
authors: L James
authors: LJ
authors: Lee
authors: Robert J
authors: RJ
authors: Lee
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism; D003959:Diamines / Q000737:chemistry* / Q000633:toxicity; D005434:Flow Cytometry; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000633:toxicity; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D053758:Nanoparticles; D010316:Particle Size; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
chemical_list: D003959:Diamines; D008055:Lipids; D034741:RNA, Small Interfering; D008156:Luciferases; C006270:avridine