title: Application of curve resolution algorithms in the study of drug photodegradation kinetics -- the example of moclobemide.
abstract: The photodegradation of moclobemide was studied in methanolic media. Ultra-HPLC (UHPLC)/MS/MS analysis proved decomposition to 4-chlorobenzamide as a major degradation product and small amounts of Ro 16-3177 (4-chloro-N-[2-[(2-hydroxyethyl)amino] ethyl]benzamide) and 2-[(4-chlorobenzylidene)amino]-N-[2-ethoxyethenyl]ethenamine. The methanolic solution was investigated spectrophotometrically in the UV region, registering the spectra during 30 min of degradation. Using reference spectra and a multivariate chemometric method (multivariate curve resolution-alternating least squares), the spectra were resolved and concentration profiles were obtained. The obtained results were in good agreement with a quantitative approach, with UHPLC-diode array detection as the reference method.
authors: robert.skibinski@am.lublin.pl
authors: Robert
authors: R
authors: Skibiński
authors: Łukasz
authors: Ł
authors: Komsta
mesh_terms: D000465:Algorithms; D001549:Benzamides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D020912:Moclobemide / Q000737:chemistry*; D010782:Photolysis; D053719:Tandem Mass Spectrometry
chemical_list: D001549:Benzamides; C084848:Ro 16-3177; D020912:Moclobemide