title: One-step electrochemically co-assembled redox-active [Ru(bpy)2(tatp)]2+-BSA-SWCNTs hybrid film for non-redox protein biosensors.
abstract: A redox-active [Ru(bpy)(2)(tatp)](2+)-BSA-SWCNTs (bpy=2,2'-bipyridine, tatp=1,4,8,9-tetra-aza-triphenylene, BSA=bovine serum albumin, SWCNTs=single-walled carbon nanotubes) hybrid film is fabricated on an indium-tin oxide (ITO) electrode via one-step electrochemical co-assembly approach. BSA is inherently dispersive and therefore served as the linking mediator of SWCNTs, which facilitate the redox reactions of [Ru(bpy)(2)(tatp)](2+) employed as a reporter of BSA. The evidences from differential pulse voltammetry, cyclic voltammetry, scanning electron microscope, emission spectroscopy and fluorescence microscope reveal that the [Ru(bpy)(2)(tatp)](2+)-BSA-SWCNTs hybrid can be electrochemically co-assembled on the ITO electrode, showing two pairs of well-defined Ru(II)-based redox waves. Furthermore, the electrochemical co-assembly of the [Ru(bpy)(2)(tatp)](2+)-BSA-SWCNTs hybrid is found to be strongly dependent on the simultaneous presence of BSA and SWCNTs, indicating a good linear response to BSA in the range from 6 to 50mgL(-1). The results from this study provide an electrochemical co-assembly method for the development of non-redox protein biosensors.
authors: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, PR China.
authors: Shi-Bo
authors: SB
authors: Ji
authors: Zhi-Hong
authors: ZH
authors: Yan
authors: Jun-Wen
authors: JW
authors: Wu
authors: Lin-Lin
authors: LL
authors: Chen
authors: Hong
authors: H
authors: Li
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D055664:Electrochemical Techniques / Q000379:methods; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis*
chemical_list: D037742:Nanotubes, Carbon; D009942:Organometallic Compounds; D010618:Phenanthrolines; C103599:bis(bipyridyl)ruthenium(II); D012710:Serum Albumin, Bovine; D015082:2,2'-Dipyridyl