title: Analysing signalling networks by mass spectrometry.
abstract: Sequence analysis of the human genome and the association of genetic aberrations with diseases have provided a rough framework whereby the impact of individual genotypes can be assessed. To fully understand the effect of individual and co-occurring genetic aberrations, as well as their individual and collected contribution to the development of diseases, it is critical to analyse the matching proteome and to determine how the organisation, expression level and function of protein networks are affected. Sensitive mass spectrometric platforms in combination with innovative workflows allow qualitative and quantitative analyses of the cellular as well as the extracellular proteome. Importantly, in addition to specifically identifying the content of the proteome, several aspects of the proteomic organisation can be analysed including protein complexes, protein modifications, enzymatic activities and subcellular/organelle localisation. Together, these measurements will provide novel insight into the biological effect of disease-causing mutations ultimately coupling genotype and phenotype.
authors: Division of Cancer Biology, Cell Communication Team, The Institute of Cancer Research, London, UK. claus.jorgensen@icr.ac.uk
authors: Claus
authors: C
authors: Jørgensen
authors: Marie
authors: M
authors: Locard-Paulet
mesh_terms: D000818:Animals; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D025941:Protein Interaction Mapping; D011499:Protein Processing, Post-Translational; D055550:Protein Stability; D020543:Proteome / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D015398:Signal Transduction
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D020543:Proteome