title: Distinct pattern of immunophenotypic features of innate and adaptive immunity as a putative signature of clinical and laboratorial status of patients with localized cutaneous leishmaniasis.
abstract: In this study, we have analysed the phenotypic features of innate/adaptive immunity of patients with localized cutaneous leishmaniasis (LCL), categorized according to their clinical/laboratorial status, including number of lesion (L1; L2–4), days of illness duration (≤60;>60) and positivity in the Montenegro skin test (MT−;MT+). Our findings highlighted a range of phenotypic features observed in patients with LCL (↑%HLA-DR+ neutrophils; ↑CD8+ HLA-DR+/CD4+ HLA-DR+ T cell ratio; ↑HLA-DR in B lymphocytes, ↑%CD23+ neutrophils, monocytes and B cells; ↑α-Leishmania IgG and ↑serum NO₂⁻ + NO₃⁻). Selective changes were observed in L1 (↑%HLA-DR+ neutrophils, ↑CD8+ HLA-DR+/CD4+ HLA-DR+ T cell ratio and ↑serum NO₂⁻ + NO₃⁻) as compared to L2–4 (↑%CD5− B cells; ↑CD23+ B cells and ↑α-Leishmania IgG). Whilst ≤60 presented a mixed profile of innate/adaptive immunity (↓%CD28+ neutrophils and ↑%CD4+ T cells), >60 showed a well-known leishmanicidal events (↑CD8+ T cells; ↑serum NO₂⁻ + NO₃⁻ and ↑α-Leishmania IgG). MT+ patients showed increased putative leishmanicidal capacity (↑%HLA-DR+ neutrophils; ↑%CD23+ monocytes; ↑CD8+ HLA-DR+/CD4+ HLA-DR+ T cell ratio and ↑ serum NO₂⁻ + NO₃⁻). Overall, a range of immunological biomarkers illustrates the complex immunological network associated with distinct clinical/laboratorial features of LCL with applicability in clinical studies.
authors: Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, FIOCRUZ/Minas, Barro Preto, Belo Horizonte, MG, Brazil.
authors: P M
authors: PM
authors: Freitas-Teixeira
authors: D
authors: D
authors: Silveira-Lemos
authors: R C
authors: RC
authors: Giunchetti
authors: A
authors: A
authors: Baratta-Masini
authors: W
authors: W
authors: Mayrink
authors: V
authors: V
authors: Peruhype-Magalhães
authors: R D R
authors: RD
authors: Rocha
authors: A C
authors: AC
authors: Campi-Azevedo
authors: A
authors: A
authors: Teixeira-Carvalho
authors: O A
authors: OA
authors: Martins-Filho
mesh_terms: D056704:Adaptive Immunity; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000097:blood / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D015415:Biomarkers / Q000097:blood; D002648:Child; D002675:Child, Preschool; D005260:Female; D006684:HLA-DR Antigens / Q000097:blood / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate; D016130:Immunophenotyping; D007223:Infant; D007892:Leishmania braziliensis / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D009566:Nitrates / Q000097:blood / Q000276:immunology; D009573:Nitrites / Q000097:blood / Q000276:immunology; D012867:Skin / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology* / Q000469:parasitology / Q000473:pathology
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D006684:HLA-DR Antigens; D009566:Nitrates; D009573:Nitrites