title: Prevalence of hepatitis B virus in chronic hepatitis B patients.
abstract: The aim of the current study was to detect HBV by Real time - PCR in chronic hepatitis B patients. Fifty-eight sera of chronic hepatitis B patients were subjected during the period March 2009 to April 2010 in Ilam cities in West of Iran. Sera assayed by real-time PCR and ELISA methods. Twenty serum samples from healthy volunteers and non-hepatitis B patients and negative for hepatitis B seromarkers served as negative controls for the study. Among fifty-eight sera, ELISA showed fifty-five (94.8%) of the samples were positive for HBsAg and three (5.2%) negative results obtained while real-time PCR specified fifty-eight (100%) positive results in chronic hepatitis B patients. HBsAg status did not necessarily reflect HBV DNA level in the serum, as 5.2% of chronic Hepatitis B patients were positive for HBV DNA but negative for HBsAg. HBV DNA was not found to be positive amongst any of the negative controls. Real time - PCR is a sensitive and reproducible assay for HBV DNA quantization.
authors: Clinical Microbiology Research Center, Ilam University Of Medical Sciences, Ilam, Iran.
authors: Nourkhoda
authors: N
authors: Sadeghifard
authors: Reza
authors: R
authors: Mohebi
authors: Zamberi
authors: Z
authors: Sekawi
authors: Sobhan
authors: S
authors: Ghafourian
authors: Hossien
authors: H
authors: Kazemian
authors: Mitra
authors: M
authors: Rezaee
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D015995:Prevalence
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens