title: Gastroprotection of suaveolol, isolated from Hyptis suaveolens, against ethanol-induced gastric lesions in Wistar rats: role of prostaglandins, nitric oxide and sulfhydryls.
abstract: Hyptis suaveolens is a medicinal plant that is, according to traditional medicine, considered useful in the treatment of gastric ulcers. Although its gastroprotective activity was reported, the active compounds have not been identified. Therefore, the aim of the present study was to identify at least one active compound potentially responsible for the gastroprotective activity of H. suaveolens by using a bioassay guided study with an ethanol-induced gastric ulcer experimental model in rats. The results show that the hexane extract had protective activity (close to 70% when using doses between 10 and 100 mg/kg), and that the compound suaveolol, isolated from this extract, was one of the active gastroprotective agents. This is the first report about the gastroprotective activity of suaveolol. Rats treated with this compound at 3, 10, 30 and 100 mg/kg showed 12.6, 21.3, 39.6 and 70.2% gastroprotection respectively. The effect elicited by suaveolol (at 100 mg/kg) was attenuated by pretreatment with either N(G)-nitro-L-arginine methyl ester (70 mg/kg, i.p.), a nitric oxide (NO) synthase inhibitor, indomethacin (10 mg/kg, s.c.), a blocker of prostaglandin synthesis, or N-ethylmaleimide (10 mg/kg, s.c.), a blocker of sulfhydryl groups. This suggests that the gastroprotective mechanism of action of this compound involves NO, prostaglandins and sulfhydryl groups.
authors: Superior Medicine School, National Polytechnic Institute, Plan de San Luis y Díaz Mirón, Colonia Santo Tomás, Delegación Miguel Hidalgo, Mexico City 11340, Mexico.
authors: Carlos
authors: C
authors: Vera-Arzave
authors: Leticia Cruz
authors: LC
authors: Antonio
authors: Jesús
authors: J
authors: Arrieta
authors: Gerardo
authors: G
authors: Cruz-Hernández
authors: Antonio Magdiel
authors: AM
authors: Velasquez-Mendez
authors: Adelfo
authors: A
authors: Reyes-Ramírez
authors: María Elena
authors: ME
authors: Sánchez-Mendoza
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D002229:Carbenoxolone / Q000494:pharmacology / Q000627:therapeutic use; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D000431:Ethanol; D005033:Ethylmaleimide / Q000494:pharmacology; D031331:Hyptis / Q000737:chemistry*; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D015394:Molecular Structure; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D011083:Polycyclic Compounds / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D011453:Prostaglandins / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*; D013438:Sulfhydryl Compounds / Q000037:antagonists & inhibitors / Q000502:physiology*; D013439:Sulfhydryl Reagents / Q000494:pharmacology
chemical_list: D000897:Anti-Ulcer Agents; D016861:Cyclooxygenase Inhibitors; D010936:Plant Extracts; D011083:Polycyclic Compounds; D011453:Prostaglandins; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; C576568:suaveolol; D009569:Nitric Oxide; D000431:Ethanol; D019001:Nitric Oxide Synthase; D002229:Carbenoxolone; D005033:Ethylmaleimide; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin