title: Heat shock protein production and immunity and altered fetal development in diabetic pregnant rats.
abstract: We evaluated associations between the concentrations of heat shock proteins (hsp60 and hsp70) and their respective antibodies, alterations in maternal reproductive performance, and fetal malformations in pregnant rats with hyperglycemia. Mild diabetes (MD) or severe diabetes (SD) was induced in Sprague-Dawley rats prior to mating; non-treated non-diabetic rats (ND) served as controls. On day 21 of pregnancy, maternal blood was analyzed for hsp60 and hsp70 and their antibodies; and fetuses were weighed and analyzed for congenital malformations. Hsp and anti-hsp levels were correlated with blood glucose levels during gestation. There was a positive correlation between hsp60 and hsp70 levels and the total number of malformations (R = 0.5908, P = 0.0024; R = 0.4877, P = 0.0134, respectively) and the number of malformations per fetus (R = 0.6103, P = 0.0015; R = 0.4875, P = 0.0134, respectively). The anti-hsp60 IgG concentration was correlated with the number of malformations per fetus (R = 0.3887, P = 0.0451) and the anti-hsp70 IgG level correlated with the total number of malformations (R = 0.3999, P = 0.0387). Moreover, both hsp and anti-hsp antibodies showed negative correlations with fetal weight. The results suggest that there is a relationship between hsp60 and hsp70 levels and their respective antibodies and alterations in maternal reproductive performance and impaired fetal development and growth in pregnancies associated with diabetes.
authors: Laboratory of Experimental Research on Gynecology and Obstetrics, Department of Gynecology and Obstetrics, UNESP-Universidade Estadual Paulista, Botucatu Medical School, Sao Paulo, Brazil.
authors: Felipe Hiroshi
authors: FH
authors: Saito
authors: Débora Cristina
authors: DC
authors: Damasceno
authors: Bruna
authors: B
authors: Dallaqua
authors: Iara Moreno
authors: IM
authors: Linhares
authors: Marilza Vieira Cunha
authors: MV
authors: Rudge
authors: Iracema
authors: I
authors: De Mattos Paranhos Calderon
authors: Steven S
authors: SS
authors: Witkin
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology; D001786:Blood Glucose / Q000032:analysis; D018834:Chaperonin 60 / Q000276:immunology / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D047109:Fetal Development; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000906:Antibodies; D001786:Blood Glucose; D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins