title: Superior osteogenic capacity of different mesenchymal stem cells for bone tissue engineering.
abstract: OBJECTIVE
  We evaluated the effect of human bone marrow stromal cells (hBMSCs), human adipose tissue-derived mesenchymal stem cells (hAD-MSCs), and umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in bone tissue engineering and identified a reliable cell source.
  
  
  METHODS
  Alkaline phosphatase (ALP) activity and quantitative polymerase chain reaction were used to evaluate osteogenic in vitro, X-ray and histologic analysis in vivo.
  
  
  RESULTS
  hBMSCs exhibited strongest ALP staining, followed by hAD-MSCs and hUC-MSCs. At 7 days, hUC-MSCs and hAD-MSCs had higher expression of collagen type I and Runt-related transcription factor 2 than hBMSCs, and hUC-MSCs showed higher osteopontin expression. Bone structure was observed in the hUC-MSC group. Defects showed good healing in the hBMSC and hAD-MSC groups. Enhanced green fluorescent protein and osteopontin were detected in newly formed bone at 8 weeks.
  
  
  CONCLUSIONS
  Our results suggested that hUC-MSCs and hAD-MSCs could be used for bone tissue engineering effectively; hUC-MSCs could serve as a new alternative cell source.
authors: School of Stomatology, Shandong University, Jinan, China; Stem Cell and Regenerative Medicine Laboratory, Beijing Institute of Transfusion Medicine, Beijing, China; Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan, China.
authors: Yong
authors: Y
authors: Wen
authors: Baoqi
authors: B
authors: Jiang
authors: Jun
authors: J
authors: Cui
authors: Guoju
authors: G
authors: Li
authors: Meijiao
authors: M
authors: Yu
authors: Fang
authors: F
authors: Wang
authors: Gairong
authors: G
authors: Zhang
authors: Xue
authors: X
authors: Nan
authors: Wen
authors: W
authors: Yue
authors: Xin
authors: X
authors: Xu
authors: Xuetao
authors: X
authors: Pei
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology; D024042:Collagen Type I / Q000378:metabolism; D050676:Core Binding Factor Alpha 2 Subunit / Q000378:metabolism; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology*; D053495:Osteopontin / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012886:Skull / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods*; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D024042:Collagen Type I; D050676:Core Binding Factor Alpha 2 Subunit; D053495:Osteopontin; D049452:Green Fluorescent Proteins; D000469:Alkaline Phosphatase