title: Molecular characterization and expression of As-nurp1 gene from Artemia sinica during development and in response to salinity and temperature stress.
abstract: Nuclear protein 1 (NURP1) is a stress-related protein and closely related to diapause in the development of Artemia. In the present paper, the full-length 568-bp cDNA sequence of the nurp1 homolog of Artemia sinica (As-nurp1) was isolated by RACE technology for the first time. The putative As-nurp1 protein consists of 66 amino acids with a basic helix-loop-helix (bHLH) motif and a bipartite nuclear localization signal (NLS). Multiple sequence alignments revealed that the putative As-nurp1 protein sequence was relatively conserved across species, especially in the bHLH domain. The expression of As-nurp1 is widely distributed during A. sinica development. This is followed by a dramatic downregulation after diapause and is newly upregulated from the larval nauplius stage. Furthermore, As-nurp1 transcripts are highly upregulated under conditions of high salinity and low temperature. These findings suggest that As-nurp1 is stress-related and may act as a protective factor in embryonic development.
authors: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors: Qiuying
authors: Q
authors: Li
authors: Qiaozhi
authors: Q
authors: Zhang
authors: Lulu
authors: L
authors: Han
authors: Zhe
authors: Z
authors: Yuan
authors: Jian
authors: J
authors: Tan
authors: Bin
authors: B
authors: Du
authors: Xiangyang
authors: X
authors: Zou
authors: Lin
authors: L
authors: Hou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D054712:Salinity; D013312:Stress, Physiological / Q000502:physiology*; D013696:Temperature
chemical_list: D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D009687:Nuclear Proteins