title: Inhibition of Pim-1 attenuates the proliferation and migration in nasopharyngeal carcinoma cells.
abstract: OBJECTIVE
  To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma (NPC) cells.
  
  
  METHODS
  Pim-1 expressions in NPC cell lines CNE1, CNE1-GL, CNE-2Z and C666-1 were examined by RT-PCR, western blotting and immunoflucesence, respectively. After CNE1, CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor, quercetagetin, the cell viability, colony formation rate and migration ability were analyzed.
  
  
  RESULTS
  Pim-1 expression was negative in well-differentiated CNE1 cells, whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells. Interestingly, CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1. Treatment of CNE1-GL and C666-1 cells with quercetagetin significantly decreased the cell viability, colony formation rate and migration ability but not the CNE1 cells.
  
  
  CONCLUSIONS
  These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration, and targeting Pim-1 may be a potential treatment for anti-Pim-1-expressed NPCs.
authors: Department of Pathology & Pathophysiology, School of Basic Medicine Science, Guangdong Medical College, Zhanjiang 524023, PR China.
authors: Wei
authors: W
authors: Jie
authors: Qi-Yi
authors: QY
authors: He
authors: Bo-Tao
authors: BT
authors: Luo
authors: Shao-Jiang
authors: SJ
authors: Zheng
authors: Yue-Qiong
authors: YQ
authors: Kong
authors: Han-Guo
authors: HG
authors: Jiang
authors: Ru-Jia
authors: RJ
authors: Li
authors: Jun-Li
authors: JL
authors: Guo
authors: Zhi-Hua
authors: ZH
authors: Shen
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015153:Blotting, Western; D002277:Carcinoma; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002867:Chromones / Q000494:pharmacology*; D047309:Flavones; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D000090063:Proto-Oncogene Mas; D051573:Proto-Oncogene Proteins c-pim-1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014410:Tumor Stem Cell Assay
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; D002867:Chromones; D047309:Flavones; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; C496634:PIM1 protein, human; D051573:Proto-Oncogene Proteins c-pim-1; C079163:quercetagetin