title: Isolation of saffold virus type 2 in green monkey kidney cells.
abstract: Saffold viruses (SAFV) have been discovered recently and they are classified into Theilovirus species in genus Cardiovirus in the Picornaviridae family. SAFV, especially those belonging to the genotype 2, have been difficult to propagate in laboratory cell lines. This study describes the successful isolation of an efficiently growing SAFV-2 strain directly from a stool specimen by standard virological methods. The availability of SAFV isolates that can be propagated to high titers is crucial to the future studies on pathogenesis and epidemiology of these novel human viruses.
authors: Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare-THL, Helsinki, Finland. soile.blomqvist@thl.fi
authors: Soile
authors: S
authors: Blomqvist
authors: Maija
authors: M
authors: Lappalainen
authors: Anja
authors: A
authors: Paananen
authors: Petri
authors: P
authors: Ylipaasto
authors: Merja
authors: M
authors: Roivainen
mesh_terms: D000818:Animals; D018188:Cardiovirus Infections / Q000662:veterinary* / Q000821:virology; D002522:Chlorocebus aethiops / Q000821:virology*; D005243:Feces / Q000821:virology; D006801:Humans; D007668:Kidney / Q000166:cytology; D008992:Monkey Diseases / Q000821:virology*; D058974:Phylogeography; D017422:Sequence Analysis, DNA; D017945:Theilovirus / Q000235:genetics / Q000302:isolation & purification*; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins