title: RBMX: a regulator for maintenance and centromeric protection of sister chromatid cohesion.
abstract: Cohesion is essential for the identification of sister chromatids and for the biorientation of chromosomes until their segregation. Here, we have demonstrated that an RNA-binding motif protein encoded on the X chromosome (RBMX) plays an essential role in chromosome morphogenesis through its association with chromatin, but not with RNA. Depletion of RBMX by RNA interference (RNAi) causes the loss of cohesin from the centromeric regions before anaphase, resulting in premature chromatid separation accompanied by delocalization of the shugoshin complex and outer kinetochore proteins. Cohesion defects caused by RBMX depletion can be detected as early as the G2 phase. Moreover, RBMX associates with the cohesin subunits, Scc1 and Smc3, and with the cohesion regulator, Wapl. RBMX is required for cohesion only in the presence of Wapl, suggesting that RBMX is an inhibitor of Wapl. We propose that RBMX is a cohesion regulator that maintains the proper cohesion of sister chromatids.
authors: Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan. sachi@rs.tus.ac.jp
authors: Sachihiro
authors: S
authors: Matsunaga
authors: Hideaki
authors: H
authors: Takata
authors: Akihiro
authors: A
authors: Morimoto
authors: Kayoko
authors: K
authors: Hayashihara
authors: Tsunehito
authors: T
authors: Higashi
authors: Kouhei
authors: K
authors: Akatsuchi
authors: Eri
authors: E
authors: Mizusawa
authors: Mariko
authors: M
authors: Yamakawa
authors: Mamoru
authors: M
authors: Ashida
authors: Tomoko M
authors: TM
authors: Matsunaga
authors: Takachika
authors: T
authors: Azuma
authors: Susumu
authors: S
authors: Uchiyama
authors: Kiichi
authors: K
authors: Fukui
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism / Q000502:physiology; D018797:Cell Cycle Proteins / Q000378:metabolism / Q000502:physiology; D002503:Centromere / Q000378:metabolism; D011508:Chondroitin Sulfate Proteoglycans / Q000378:metabolism / Q000502:physiology; D002842:Chromatids / Q000378:metabolism / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism / Q000502:physiology; D004268:DNA-Binding Proteins; D016195:G2 Phase; D006367:HeLa Cells; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism / Q000502:physiology; D010750:Phosphoproteins / Q000378:metabolism / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000378:metabolism / Q000502:physiology; D034622:RNA Interference; D000097722:Cohesins
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D011508:Chondroitin Sulfate Proteoglycans; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; C429022:RAD21 protein, human; C510272:RBMX protein, human; C492318:SMC3 protein, human; C488092:WAPL protein, human; D000097722:Cohesins