title: Altered microRNA expression in bovine subcutaneous and visceral adipose tissues from cattle under different diet.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are a class of molecular regulators found to participate in numerous biological processes, including adipogenesis in mammals. This study aimed to evaluate the differences of miRNA expression between bovine subcutaneous (backfat) and visceral fat depots (perirenal fat) and the dietary effect on miRNA expression in these fat tissues.
  
  
  RESULTS
  Fat tissues were collected from 16 Hereford×Aberdeen Angus cross bred steers (15.5 month old) fed a high-fat diet (5.85% fat, n = 8) or control diet (1.95% fat, n = 8). Total RNA from each animal was subjected to miRNA microarray analysis using a customized Agilent miRNA microarray containing 672 bovine miRNA probes. Expression of miRNAs was not equal between fat depots as well as diets: 207 miRNAs were detected in both fat depots, while 37 of these were found to be tissue specific; and 169 miRNAs were commonly expressed under two diets while 75 were diet specific. The number of miRNAs detected per animal fed the high fat diet was higher than those fed control diet (p = 0.037 in subcutaneous fat and p = 0.002 visceral fat). Further qRT-PCR analysis confirmed that the expression of some miRNAs was highly influenced by diet (miR-19a, -92a, -92b, -101, -103, -106, -142-5p, and 296) or fat depot (miR-196a and -2454).
  
  
  CONCLUSIONS
  Our results revealed that the miRNA may differ among adipose depots and level of fat in the diet, suggesting that miRNAs may play a role in the regulation of bovine adipogenesis.
authors: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
authors: Josue Moura
authors: JM
authors: Romao
authors: Weiwu
authors: W
authors: Jin
authors: Maolong
authors: M
authors: He
authors: Tim
authors: T
authors: McAllister
authors: Le Luo
authors: LL
authors: Guan
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D002417:Cattle / Q000235:genetics*; D004032:Diet / Q000662:veterinary*; D005247:Feeding Behavior; D005786:Gene Expression Regulation; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D050356:Lipid Metabolism / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D019655:Quantitative Trait, Heritable; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050151:Subcutaneous Fat / Q000378:metabolism*
chemical_list: D035683:MicroRNAs