title: Simultaneous injection effective mixing flow analysis of urinary albumin using dye-binding reaction.
abstract: A new four-channel simultaneous injection effective mixing flow analysis (SIEMA) system has been assembled for the determination of urinary albumin. The SIEMA system consisted of a syringe pump, two 5-way cross connectors, four holding coils, five 3-way solenoid valves, a 50-cm long mixing coil and a spectrophotometer. Tetrabromophenol blue anion (TBPB) in Triton X-100 micelle reacted with albumin at pH 3.2 to form a blue ion complex with a λ(max) 625nm. TBPB, Triton X-100, acetate buffer and albumin standard solutions were aspirated into four individual holding coils by a syringe pump and then the aspirated zones were simultaneously pushed in the reverse direction to the detector flow cell. Baseline drift, due to adsorption of TBPB-albumin complex on the wall of the hydrophobic PTFE tubing, was minimized by aspiration of Triton X-100 and acetate buffer solutions between samples. The calibration graph was linear in the range of 10-50μg/mL and the detection limit for albumin (3σ) was 0.53μg/mL. The RSD (n=11) at 30μg/mL was 1.35%. The sample throughput was 37/h. With a 10-fold dilution, interference from urine matrix was removed. The proposed method has advantages in terms of simple automation operation and short analysis time.
authors: Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan.
authors: Nuanlaor
authors: N
authors: Ratanawimarnwong
authors: Kraingkrai
authors: K
authors: Ponhong
authors: Norio
authors: N
authors: Teshima
authors: Duangjai
authors: D
authors: Nacapricha
authors: Kate
authors: K
authors: Grudpan
authors: Tadao
authors: T
authors: Sakai
authors: Shoji
authors: S
authors: Motomizu
mesh_terms: D000418:Albumins / Q000032:analysis* / Q000737:chemistry*; D000818:Animals; D001978:Bromphenol Blue / Q000737:chemistry*; D002417:Cattle; D004396:Coloring Agents / Q000737:chemistry*; D003920:Diabetes Mellitus / Q000652:urine; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D013053:Spectrophotometry; D013997:Time Factors; D016482:Urinalysis / Q000379:methods*
chemical_list: D000418:Albumins; D004396:Coloring Agents; D001978:Bromphenol Blue