title: Expression of leucocyte function-associated antigen-1 and intercellular adhesion molecule-1 in the lungs of pigs infected with Actinobacillus pleuropneumoniae.
abstract: The aim of this study was to determine the expression of leucocyte function-associated antigen (LFA)-1 (CD11a/CD18) by neutrophils and intercellular adhesion molecule (ICAM)-1 (CD54) by endothelial cells in the lungs of pigs that had been infected experimentally with Actinobacillus pleuropneumoniae. Sixty-four 7-week-old conventional pigs were allocated randomly into infected (n = 40) or control (n = 24) groups. Five infected and three uninfected pigs were killed at 3, 6, 9, 12, 24, 36, 48 and 60 h post inoculation (hpi). Strong immunohistochemical expression of LFA-1 and ICAM-1 was detected frequently in neutrophils in the alveolar space and in endothelial cells in the capillaries of the alveolar septa, respectively. LFA-1 and ICAM-1 expression appeared to correlate with the onset of neutrophil infiltration into the alveolar space. The interaction between ICAM-1 and LFA-1 may be associated with the adherence of neutrophils to vascular endothelium, thereby permitting transmigration of these cells into inflamed lung.
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea.
authors: Y
authors: Y
authors: Oh
authors: Y
authors: Y
authors: Ha
authors: K
authors: K
authors: Han
authors: H W
authors: HW
authors: Seo
authors: I
authors: I
authors: Kang
authors: C
authors: C
authors: Park
authors: S C
authors: SC
authors: Kim
authors: S-H
authors: SH
authors: Kim
authors: C
authors: C
authors: Chae
mesh_terms: D000189:Actinobacillus Infections / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000302:isolation & purification*; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000382:microbiology* / Q000473:pathology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction / Q000502:physiology; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1