title: A cardiac tissue-specific binding agent of troponin I.
abstract: A fluorescently labeled, biphenylalanine-rich peptide was identified as a should be cardiac troponin I-specific binding agent with preferential staining affinity to myocardium tissues and extremely low staining to other stromal components. Fluorescence images demonstrate that this new peptide is an excellent contrast agent useful for examining troponin I structural distribution and expression density within heart tissue sections. It provides a clear contrast between myocardial cells and the surrounding collagen matrix.
authors: Department of Translational Imaging, Methodist Hospital Research Institute, Weill Cornell Medical College, Houston, TX, USA. ctung@tmhs.org
authors: Wael R
authors: WR
authors: Abd-Elgaliel
authors: Ching-Hsuan
authors: CH
authors: Tung
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005456:Fluorescent Dyes; D006651:Histocytochemistry / Q000379:methods*; D007202:Indicators and Reagents / Q000138:chemical synthesis* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000648:ultrastructure; D009206:Myocardium / Q000648:ultrastructure*; D032383:Myocytes, Cardiac / Q000648:ultrastructure*; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000378:metabolism; D011485:Protein Binding; D019210:Troponin I / Q000032:analysis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D009842:Oligopeptides; D019210:Troponin I