title: Differential scanning microcalorimetry of intrinsically disordered proteins.
abstract: Ultrasensitive differential scanning calorimetry (DSC) is an indispensable thermophysical technique enabling to get direct information on enthalpies accompanying heating/cooling of dilute biopolymer solutions. The thermal dependence of protein heat capacity extracted from DSC data is a valuable source of information on intrinsic disorder level of a protein. Application details and limitations of DSC technique in exploration of protein intrinsic disorder are described.
authors: Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia. permyakov.s@gmail.com
authors: Sergei E
authors: SE
authors: Permyakov
mesh_terms: D002021:Buffers; D002152:Calorimetry, Differential Scanning / Q000379:methods*; D006358:Hot Temperature; D010984:Platinum / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D002021:Buffers; D011506:Proteins; D010984:Platinum