title: Fast screening for antioxidant properties of flavonoids from Pterogyne nitens using electrochemical methods.
abstract: A fast, low-cost, convenient, and especially sensitive voltammetric screening approach for the study of the antioxidant properties of isoquercitrin and pedalitin from Pterogyne nitens is suggested in this work. These flavonoids were investigated for their redox properties using cyclic voltammetry in nonaqueous media using N,N-dimethylformamide and tetrabutylammonium tetrafluorborate as the supporting electrolyte, a glassy carbon working electrode, A6(see symbol in text)AgCI reference electrode, and Pt bare wire counter electrode. The comparative analysis of the activity of rutin has also been carried out. Moreover, combining HPLC with an electrochemical detector allowed qualitative and quantitative detection of micromolecules (e.g., isoquercitrin and pedalitin) that showed antioxidant activities. These results were then correlated to the inhibition of beta-carotene bleaching determined by TLC autographic assay and to structural features of the flavonoids.
authors: Federal University of Viçosa-UFV, Dept. of Chemistry, Vigosa, MG, Brazil. leo.okumura@ufv.br
authors: Leonardo Luiz
authors: LL
authors: Okumura
authors: Luis Octávio
authors: LO
authors: Regasini
authors: Daniara Cristina
authors: DC
authors: Fernandes
authors: Dulce Helena Siqueira
authors: DH
authors: da Silva
authors: Maria Valnice Boldrin
authors: MV
authors: Zanoni
authors: Vanderlan da Silva
authors: Vda S
authors: Bolzani
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D055664:Electrochemical Techniques / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D047309:Flavones / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology; D012431:Rutin / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D047309:Flavones; D005419:Flavonoids; C510777:pedalitin; C016527:isoquercitrin; D012431:Rutin; D011794:Quercetin