title: Evaluation of lipid removal from animal fats for the determination of organochlorine, pesticides, and polychlorinated biphenyl indicators by gas chromatography with electron capture detector.
abstract: For cleanup of animal fat before GC analysis of organochlorine pesticides and polychlorinated biphenyls, freezing-lipid filtration with solid-phase extraction and matrix solid-phase dispersion were evaluated to replace the official Brazilian methodology that uses preparative alumina column chromatography. General drawbacks associated with this last technique, such as the use of large amounts of solvent, laborious and time-consuming procedure could be avoided by using these alternative approaches. Experiments were carried out to study the performance by using different combinations of sorbents and elution solvents. Efficiency of alternative extraction methods in terms of fat removal and recovery capability was monitored by gravimetry, TLC, and GC with electron capture detection. Freezing-lipid filtration with solid-phase extraction afforded better clean up efficiency with recoveries in a range of 54.5 to 103.6% with the relative standard deviation of less than 10% for all compounds under investigation.
authors: Laboratory of Pesticides and Veterinary Residues - Ministry of Agriculture, Livestock and Food Supply of Brazil, Brazil. adrianasantosandrade@gmail.com
authors: Adriana S
authors: AS
authors: Andrade
authors: Doriane
authors: D
authors: Sacheto
authors: Rodrigo B
authors: RB
authors: Hoff
authors: Tania M
authors: TM
authors: Pizzolato
mesh_terms: D000818:Animals; D001938:Brazil; D002417:Cattle; D002645:Chickens; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods; D005223:Fats / Q000737:chemistry*; D006736:Horses; D006843:Hydrocarbons, Chlorinated / Q000032:analysis / Q000302:isolation & purification*; D008055:Lipids / Q000032:analysis / Q000302:isolation & purification*; D010573:Pesticide Residues / Q000032:analysis / Q000302:isolation & purification*; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D013552:Swine
chemical_list: D005223:Fats; D006843:Hydrocarbons, Chlorinated; D008055:Lipids; D010573:Pesticide Residues; D011078:Polychlorinated Biphenyls