title: MicroRNA-10b targets E-cadherin and modulates breast cancer metastasis.
abstract: BACKGROUND
  Recent studies have suggested that microRNA-10b (miR-10b) acts as a promoter of metastasis in breast cancer, although the underlying mechanism remains largely unknown. In this study, we provide the first evidence that E-cadherin (E-cad) is a potential target of miR-10b.
  
  
  METHODS
  By applying gain-of-function and loss-of-function approaches in the metastatic breast cancer cell line MDA-MB-231, we demonstrated that miR-10b is necessary and sufficient to regulate the cellular expression of E-cad and in vitro tumor cell invasion.
  
  
  RESULTS
  Comparative expression analysis of miR-10b in benign breast lesions (N=16), primary breast cancers (N=21), and metastatic breast carcinomas (N=23) revealed that miR-10b transcription was uniquely up-regulated in metastatic cancers. The expression level of miR-10b positively correlated with tumor size, pathological grading, clinical staging, lymph node metastasis, Her2-positivity and tumor proliferation, but was negatively associated with estrogen receptor-positivity, progesterone receptor-positivity and E-cad mRNA and protein levels.
  
  
  CONCLUSIONS
  These findings indicate the existence of a novel E-cadherin-related mechanism by which miR-10b modulates breast cancer metastasis. In addition, miR-10b may be a useful biomarker of advanced progression and metastasis of breast cancer.
authors: Department of Medical Oncology, Central Hospital of Xuzhou, Affiliated Xuzhou Hospital, Medical College of Southeast University, Xuzhou, China.
authors: Yong
authors: Y
authors: Liu
authors: Jing
authors: J
authors: Zhao
authors: Pei-Ying
authors: PY
authors: Zhang
authors: Yu
authors: Y
authors: Zhang
authors: San-Yuan
authors: SY
authors: Sun
authors: Shi-Ying
authors: SY
authors: Yu
authors: Qing-Song
authors: QS
authors: Xi
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016014:Linear Models; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D008969:Molecular Sequence Data; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D015854:Up-Regulation / Q000235:genetics
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C547183:MIRN10 microRNA, human; D035683:MicroRNAs