title: Gene amplification of ZNF217 located at chr20q13.2 is associated with lymph node metastasis in ovarian clear cell carcinoma.
abstract: BACKGROUND
  Recently we reported that amplification of the Zinc Finger Protein 217 (ZNF217) gene adversely affects survival of patients with ovarian clear cell carcinoma. This study sought to determine the mechanism by which ZNF217 amplification affects patient survival.
  
  
  METHODS
  Fluorescence in situ hybridization (FISH) was used to detect ZNF217 gene amplification status and ZNF217-specific siRNA was used to inactivate ZNF217 for in vitro biological analyses.
  
  
  RESULTS
  We found ZNF217 gene amplification to be significantly correlated with lymph node metastasis (p<0.05) in ovarian clear cell carcinoma. Profound inhibition of cell migration and invasion was observed in siRNA-treated cells with ZNF217 amplification, compared to cells without amplification.
  
  
  CONCLUSIONS
  These findings provide new insight into the biological role of ZNF217 gene amplification in ovarian clear cell carcinoma. Additionally, our observations have an important therapeutic implication for patients with ovarian clear cell carcinomas with ZNF217 amplification, as these patients may potentially benefit from ZNF217 targeted-therapy.
authors: Department of Obstetrics, Shimane University School of Medicine, Shimane, Japan.
authors: Mohammed Tanjimur
authors: MT
authors: Rahman
authors: Kentaro
authors: K
authors: Nakayama
authors: Munmun
authors: M
authors: Rahman
authors: Hiroshi
authors: H
authors: Katagiri
authors: Atsuko
authors: A
authors: Katagiri
authors: Tomoka
authors: T
authors: Ishibashi
authors: Masako
authors: M
authors: Ishikawa
authors: Kouji
authors: K
authors: Iida
authors: Naomi
authors: N
authors: Nakayama
authors: Yoshiro
authors: Y
authors: Otsuki
authors: Satoru
authors: S
authors: Nakayama
authors: Kohji
authors: K
authors: Miyazaki
mesh_terms: D002890:Chromosomes, Human, Pair 20; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008207:Lymphatic Metastasis; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology*; D034741:RNA, Small Interfering; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D015534:Trans-Activators; C113698:ZNF217 protein, human