title: Overexpression of the wheat salt tolerance-related gene TaSC enhances salt tolerance in Arabidopsis.
abstract: A novel gene named TaSC was cloned from salt-tolerant wheat. Northern blot showed that the expression of TaSC in salt-tolerant wheat was up-regulated after salt stress. Real-time quantitative PCR analyses showed that TaSC expression was induced by salt and ABA in wheat. Localization analysis showed that TaSC proteins were localized to the plasma membrane in transgenic Arabidopsis thaliana. The overexpression of TaSC in Col-0 and atsc (SALK_072220) Arabidopsis strains resulted in increased salt tolerance of the transgenic plants. TaSC overexpression in Col-0 and atsc signiﬁcantly up-regulated the expression of AtFRY1, AtSAD1, and AtCDPK2. AtCDPK2 overexpression in atsc rescued the salt-sensitive phenotype of atsc. The TaSC gene may improve plant salt tolerance by acting via the CDPK pathway.
authors: College of Life Science, Hebei Normal University, No. 20 Road East of 2nd Ring South, Yuhua District, Shijiazhuang 050024  China.
authors: Xi
authors: X
authors: Huang
authors: Yang
authors: Y
authors: Zhang
authors: Bo
authors: B
authors: Jiao
authors: Guiping
authors: G
authors: Chen
authors: Shenghe
authors: S
authors: Huang
authors: Feng
authors: F
authors: Guo
authors: Yinzhu
authors: Y
authors: Shen
authors: Zhanjing
authors: Z
authors: Huang
authors: Baocun
authors: B
authors: Zhao
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002462:Cell Membrane; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010641:Phenotype; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D055049:Salt Tolerance / Q000235:genetics*; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology; D014908:Triticum / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D015854:Up-Regulation / Q000235:genetics
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid