title: Scp160-dependent mRNA trafficking mediates pheromone gradient sensing and chemotropism in yeast.
abstract: mRNAs encoding polarity and secretion factors (POLs) target the incipient bud site in yeast for localized translation during division. In pheromone-treated cells we now find that these mRNAs are also localized to the yeast-mating projection (shmoo) tip. However, in contrast to the budding program, neither the She2 nor She3 proteins are involved. Instead, the Scp160 RNA-binding protein binds POL and mating pathway mRNAs and regulates their spatial distribution in a Myo4- and cortical ER-dependent fashion. RNA binding by Scp160 is stimulated by activation of Gpa1, the G protein α subunit regulated by the pheromone receptor, and is required for pheromone gradient sensing, as well as subsequent chemotropic growth and cell-cell mating. These effects are incurred independently of obvious changes in translation; thus, mRNA trafficking is required for chemotropism and completion of the mating program. This is, to our knowledge, the first demonstration of ligand-activated RNA targeting in the development of a simple eukaryote.
authors: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
authors: Rita
authors: R
authors: Gelin-Licht
authors: Saurabh
authors: S
authors: Paliwal
authors: Patrick
authors: P
authors: Conlon
authors: Andre
authors: A
authors: Levchenko
authors: Jeffrey E
authors: JE
authors: Gerst
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D016764:Cell Polarity / Q000502:physiology; D002633:Chemotaxis / Q000502:physiology*; D044385:GTP-Binding Protein alpha Subunits / Q000502:physiology; D018995:Myosin Heavy Chains / Q000502:physiology; D024701:Myosin Type V / Q000502:physiology; D010675:Pheromones / Q000502:physiology*; D012333:RNA, Messenger / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology*; D018523:Tropism / Q000502:physiology*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D044385:GTP-Binding Protein alpha Subunits; C098732:Myo4 protein, S cerevisiae; D010675:Pheromones; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C097262:SCP160 protein, S cerevisiae; C103130:SEC3 protein, S cerevisiae; C471175:SHE2 protein, S cerevisiae; C471176:SHE3 protein, S cerevisiae; C116430:SRO7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D024701:Myosin Type V; D018995:Myosin Heavy Chains