title: Low melting point pyridinium ionic liquid pretreatment for enhancing enzymatic saccharification of cellulosic biomass.
abstract: The potential of 1-hexylpyridinium chloride ([Hpy][Cl]), to pretreat cellulosic feedstocks was investigated using microcrystalline cellulose (Avicel) and Bagasse at 80 °C or 100 °C. Short [Hpy][Cl] pretreatments, <30 min, at lower temperature accelerate subsequent enzymatic saccharification of Avicel. Over 95% conversion of pretreated Avicel to glucose was attained after 24h enzymatic saccharification under optimal conditions, whereas regenerated Bagasse showed 1-3-fold higher conversion than untreated biomass. FT-IR analysis of both Avicel and Bagasse samples pretreated with [Hpy][Cl] or 1-ethyl-3-methyimidazolium acetate ([Emim][OAc]) revealed that these ionic liquids behaved differently during pretreatment. [Hpy][Cl] pretreatment for an extended duration (180 min) released mono- and disaccharides without using cellulase enzymes, suggesting [Hpy][Cl] has capability for direct saccharification of cellulosic feedstocks. On the basis of the results obtained, [Hpy][Cl] pretreatment enhanced initial reaction rates in enzymatic saccharification by either crystalline polymorphic alteration of cellulose or partial degradation of the crystalline cellulosic fraction in biomass.
authors: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan.
authors: Uju
authors: Aya
authors: A
authors: Nakamoto
authors: Yasuhiro
authors: Y
authors: Shoda
authors: Masahiro
authors: M
authors: Goto
authors: Wataru
authors: W
authors: Tokuhara
authors: Yoshiyuki
authors: Y
authors: Noritake
authors: Satoshi
authors: S
authors: Katahira
authors: Nobuhiro
authors: N
authors: Ishida
authors: Chiaki
authors: C
authors: Ogino
authors: Noriho
authors: N
authors: Kamiya
mesh_terms: D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D052578:Ionic Liquids / Q000494:pharmacology*; D008031:Lignin / Q000032:analysis; D011726:Pyridinium Compounds / Q000494:pharmacology*; D059027:Recycling; D044366:Transition Temperature
chemical_list: C545843:1-hexylpyridinium; D052578:Ionic Liquids; D011726:Pyridinium Compounds; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D002480:Cellulase; C109691:microcrystalline cellulose