title: Assessment of the clinical significance of antigenic and functional levels of α1-proteinase inhibitor (α1-Pi) in infiltrating ductal breast carcinomas.
abstract: OBJECTIVE
  To determine the clinical significance of α1-proteinase inhibitor (α1-Pi) in infiltrating ductal breast carcinoma patients.
  
  
  METHODS
  Serum levels of α1-Pi, tryptic specific inhibitory capacity and α1-Pi circulating immune complexes were determined using radial immunodiffusion, BAPNA assays and ELISA, respectively. 2-DE-MS and immunohistochemistry were performed to examine α1-Pi protein expression.
  
  
  RESULTS
  A decreased serum level of α1-Pi was found among breast cancer patients in comparison to controls. In addition, we found a significantly decreased mean level of α1-Pi in the node metastatic group when compared to node negative patients. However, the functional activity of the inhibitor did not decrease proportionately. Through 2-DE analyses, a differential expression of α1-Pi isoforms according to tumor stage and node metastatic development was found.
  
  
  CONCLUSIONS
  Both α1-Pi levels and specific activity could be a source of complementary clinical information and may provide useful information for a better understanding of the mechanisms of metastasis.
authors: Laboratoire d'Immuno-Oncologie Moléculaire, Faculté de Médecine de Monastir, Tunisia.
authors: Amel Ben
authors: AB
authors: Anes
authors: Hela Ben
authors: HB
authors: Nasr
authors: Philippe
authors: P
authors: Hammann
authors: Lauriane
authors: L
authors: Kuhn
authors: Mounir
authors: M
authors: Trimeche
authors: Bechr
authors: B
authors: Hamrita
authors: Iheb
authors: I
authors: Bougmiza
authors: Anouar
authors: A
authors: Chaieb
authors: Hedi
authors: H
authors: Khairi
authors: Karim
authors: K
authors: Chahed
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000097:blood* / Q000276:immunology; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D001943:Breast Neoplasms / Q000097:blood* / Q000276:immunology / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000097:blood* / Q000276:immunology / Q000556:secondary; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D020033:Protein Isoforms / Q000097:blood / Q000276:immunology; D059748:Proteolysis; D012372:ROC Curve; D014357:Trypsin / Q000737:chemistry; D014361:Trypsin Inhibitors / Q000097:blood / Q000276:immunology; D000515:alpha 1-Antitrypsin / Q000097:blood* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D010446:Peptide Fragments; D020033:Protein Isoforms; D014361:Trypsin Inhibitors; D000515:alpha 1-Antitrypsin; D014357:Trypsin