title: Feline bone marrow-derived mesenchymal stromal cells (MSCs) show similar phenotype and functions with regards to neuronal differentiation as human MSCs.
abstract: Mesenchymal stromal cells (MSCs) show promise for treatment of a variety of neurological and other disorders. Cat has a high degree of linkage with the human genome and has been used as a model for analysis of neurological disorders such as stroke, Alzheimer's disease and motor disorders. The present study was designed to characterize bone marrow-derived MSCs from cats and to investigate the capacity to generate functional peptidergic neurons. MSCs were expanded with cells from the femurs of cats and then characterized by phenotype and function. Phenotypically, feline and human MSCs shared surface markers, and lacked hematopoietic markers, with similar morphology. As compared to a subset of human MSCs, feline MSCs showed no evidence of the major histocompatibility class II. Since the literature suggested Stro-1 as an indicator of pluripotency, we compared early and late passages feline MSCs and found its expression in >90% of the cells. However, the early passage cells showed two distinct populations of Stro-1-expressing cells. At passage 5, the MSCs were more homogeneous with regards to Stro-1 expression. The passage 5 MSCs differentiated to osteogenic and adipogenic cells, and generated neurons with electrophysiological properties. This correlated with the expression of mature neuronal markers with concomitant decrease in stem cell-associated genes. At day 12 induction, the cells were positive for MAP2, Neuronal Nuclei, tubulin βIII, Tau and synaptophysin. This correlated with electrophysiological maturity as presented by excitatory postsynaptic potentials (EPSPs). The findings indicate that the cat may constitute a promising biomedical model for evaluation of novel therapies such as stem cell therapy in such neurological disorders as Alzheimer's disease and stroke.
authors: Department of Medicine-Hematology-Oncology University of Medicine and Dentistry, New Jersey Medical School, Newark, NJ 07103, USA.
authors: Jessian L
authors: JL
authors: Munoz
authors: Steven J
authors: SJ
authors: Greco
authors: Shyam A
authors: SA
authors: Patel
authors: Lauren S
authors: LS
authors: Sherman
authors: Suresh
authors: S
authors: Bhatt
authors: Rekha S
authors: RS
authors: Bhatt
authors: Jeffrey A
authors: JA
authors: Shrensel
authors: Yan-Zhong
authors: YZ
authors: Guan
authors: Guiqin
authors: G
authors: Xie
authors: Jiang-Hong
authors: JH
authors: Ye
authors: Pranela
authors: P
authors: Rameshwar
authors: Allan
authors: A
authors: Siegel
mesh_terms: D050156:Adipogenesis; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002415:Cats; D002478:Cells, Cultured; D019706:Excitatory Postsynaptic Potentials; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D010641:Phenotype; D013045:Species Specificity
chemical_list: D000954:Antigens, Surface; C525591:STRO-1 antigen, human