title: Effects of silica nanoparticle supported ionic liquid as additive on thermal reversibility of human carbonic anhydrase II.
abstract: Silica nanoparticle supported imidazolium ionic liquid [SNImIL] was synthesized and utilized as a biocompatible additive for studying the thermal reversibility of human carbonic anhydrase II (HCA II). For this purpose, we prepared additive by modification of nanoparticles through the grafting of ionic liquids on the surface of nanoparticles (SNImIL). The SNImIL were fully characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and thermo gravimetric analysis. The characterization of HCA II was investigated by various techniques including UV-vis and ANS fluorescence spectrophotometry, differential scanning calorimetry, and docking study. SNImIL induced disaggregation, enhanced protein stability and increased thermal reversibility of HCA II by up to 42% at pH 7.75.
authors: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors: Azadeh
authors: A
authors: Fallah-Bagheri
authors: Ali Akbar
authors: AA
authors: Saboury
authors: Leila
authors: L
authors: Ma'mani
authors: Mohammad
authors: M
authors: Taghizadeh
authors: Reza
authors: R
authors: Khodarahmi
authors: Samira
authors: S
authors: Ranjbar
authors: Mousa
authors: M
authors: Bohlooli
authors: Abbas
authors: A
authors: Shafiee
authors: Alireza
authors: A
authors: Foroumadi
authors: Nader
authors: N
authors: Sheibani
authors: Ali Akbar
authors: AA
authors: Moosavi-Movahedi
mesh_terms: D024402:Carbonic Anhydrase II / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D062105:Molecular Docking Simulation; D053758:Nanoparticles / Q000737:chemistry*; D055503:Protein Multimerization / Q000187:drug effects; D020836:Protein Structure, Quaternary; D012822:Silicon Dioxide / Q000737:chemistry*; D013696:Temperature
chemical_list: D007093:Imidazoles; D052578:Ionic Liquids; D012822:Silicon Dioxide; D024402:Carbonic Anhydrase II