title: LKTA and PlpE small fragments fusion protein protect against Mannheimia haemolytica challenge.
abstract: Bovine respiratory disease (BRD) complex is a major cause of economic losses for the cattle backgrounding and feedlot industries. Mannheimia haemolytica is considered the most important pathogen associated with this disease. Vaccines against M. haemolytica have been prepared and used for many decades, but traditional bacterins have failed to demonstrate effective protection and their use has often exacerbated disease in vaccinated animals. Thus, the BRD complex continues to exert a strong adverse effect on the health and wellbeing of stocker and feeder cattle. Therefore, generation of recombinant proteins has been helpful in formulating enhanced vaccines against M. haemolytica, which could confer better protection against BRD. In the present study, we formulated a vaccine preparation enriched with recombinant small fragments of leukotoxin A (LKTA) and outer-membrane lipoprotein (PlpE) proteins, and demonstrated its ability to generate high antibody titers in rabbits and sheep, which protected against M. haemolytica bacterial challenge in mice.
authors: Laboratorio de Desarrollo y Regeneración Neural, Instituto de Neurobiología, Departamento de Biología Celular y Molecular, CUCBA, Universidad de Guadalajara, camino Ing. R. Padilla Sánchez, 2100, Las Agujas, Zapopan 44600, Mexico.
authors: Carolina
authors: C
authors: Guzmán-Brambila
authors: Saray
authors: S
authors: Quintero-Fabián
authors: Celia
authors: C
authors: González-Castillo
authors: Álvaro
authors: Á
authors: de Obeso-Fernández del Valle
authors: Beatriz
authors: B
authors: Flores-Samaniego
authors: Germán
authors: G
authors: de la Mora
authors: Argelia E
authors: AE
authors: Rojas-Mayorquín
authors: Daniel
authors: D
authors: Ortuño-Sahagún
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006460:Hemolysin Proteins / Q000737:chemistry / Q000276:immunology*; D008074:Lipoproteins / Q000737:chemistry / Q000276:immunology*; D016978:Mannheimia haemolytica / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016871:Pasteurellaceae Infections / Q000517:prevention & control / Q000662:veterinary*; D011817:Rabbits; D011994:Recombinant Proteins; D012756:Sheep
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D006460:Hemolysin Proteins; D008074:Lipoproteins; C116100:PlpE protein, Pasteurella haemolytica; D011994:Recombinant Proteins; C102677:lktA protein, bacteria