title: High frequency of t(14;18) in Hodgkin's lymphoma associated with follicular lymphoma.
abstract: t(14;18) is a common cytogenetic abnormality in B-cell lymphoma, especially in follicular lymphoma (FL), but is rarely seen in Hodgkin's lymphoma (HL). However, due to the small number of cases, the incidence of t(14;18) in HL associated with FL remains unclear. In this study, we applied chromogenic in situ hybridization and fluorescence in situ hybridization for t(14;18) on paraffin-embedded tissue from four HL associated with FL cases and 11 HL cases without a history of FL for comparison. t(14;18) was present in all of the three successfully-tested HL associated with FL cases and one case without a history of FL. The frequency of t(14;18) was significantly high in HL associated with FL (p = 0.013). All Hodgkin's and Reed-Sternberg (HRS) cells having t(14;18) showed immunoreactivity for BCL2 and were negatively stained for nuclear factor-κB (NF-κB), even in Epstein-Barr virus (EBV)-infected cases. However, HRS cells without t(14;18) showed BCL2 and NF-κB immunoreactivity in 33% and 57% of cases, respectively. There was an inverse correlation between t(14;18) and NF-κB. In conclusion, we assume the incidence of t(14;18) in HL associated with FL is higher than previously believed and BCL2 expression derived from t(14;18) may play a role in the pathogenesis of HL associated with FL.
authors: Department of Pathology, School of Medicine, Kurume University, Kurume city, Fukuoka, Japan. yoshida_maki@med.kurumeu.ac.jp
authors: Maki
authors: M
authors: Yoshida
authors: Ayako
authors: A
authors: Ichikawa
authors: Hiroaki
authors: H
authors: Miyoshi
authors: Masanori
authors: M
authors: Takeuchi
authors: Yoshizo
authors: Y
authors: Kimura
authors: Daisuke
authors: D
authors: Nino
authors: Koichi
authors: K
authors: Ohshima
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification / Q000502:physiology; D006689:Hodgkin Disease / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008224:Lymphoma, Follicular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D009378:Neoplasms, Multiple Primary; D016612:Paraffin Embedding; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis; D016539:Reed-Sternberg Cells / Q000473:pathology; D014178:Translocation, Genetic
chemical_list: D014408:Biomarkers, Tumor; C099537:Epstein-Barr virus encoded RNA 1; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D012367:RNA, Viral