title: More accurate matrix-matched quantification using standard superposition method for herbal medicines.
abstract: Various analytical technologies have been developed for quantitative determination of marker compounds in herbal medicines (HMs). One important issue is matrix effects that must be addressed in method validation for different detections. Unlike biological fluids, blank matrix samples for calibration are usually unavailable for HMs. In this work, practical approaches for minimizing matrix effects in HMs analysis were proposed. The matrix effects in quantitative analysis of five saponins from Panax notoginseng were assessed using high-performance liquid chromatography (HPLC). Matrix components were found to interfere with the ionization of target analytes when mass spectrometry (MS) detection were employed. To compensate the matrix signal suppression/enhancement, two matrix-matched methods, standard addition method with the target-knockout extract and standard superposition method with a HM extract were developed and tested in this work. The results showed that the standard superposition method is simple and practical for overcoming matrix effects for quantitative analysis of HMs. Moreover, the interference components were observed to interfere with light scattering of target analytes when evaporative light scattering detection (ELSD) was utilized for quantitative analysis of HMs but was not indicated when Ultraviolet detection (UV) were employed. Thus, the issue of interference effects should be addressed and minimized for quantitative HPLC-ELSD and HPLC-MS methodologies for quality control of HMs.
authors: State Key Laboratory of Natural Medicines-China Pharmaceutical University, Nanjing 210009, China.
authors: Ying
authors: Y
authors: Liu
authors: Xiao-Wei
authors: XW
authors: Shi
authors: E-Hu
authors: EH
authors: Liu
authors: Long-Sheng
authors: LS
authors: Sheng
authors: Lian-Wen
authors: LW
authors: Qi
authors: Ping
authors: P
authors: Li
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D053723:Panax notoginseng / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet
chemical_list: D010936:Plant Extracts; D012503:Saponins