title: In planta localization and interactions of impatiens necrotic spot tospovirus proteins.
abstract: Impatiens necrotic spot tospovirus (INSV) is a significant pathogen of ornamentals. The tripartite negative- and ambi-sense RNA genome encodes six proteins that are involved in cytoplasmic replication, movement, assembly, insect transmission and defence. To gain insight into the associations of these viral proteins, we determined their intracellular localization and interactions in living plant cells. Nucleotide sequences encoding the nucleoprotein N, non-structural proteins NSs and NSm, and glycoproteins Gn and Gc of a Kentucky isolate of INSV were amplified by RT-PCR, cloned, sequenced and transiently expressed as fusions with autofluorescent proteins in leaf epidermal cells of Nicotiana benthamiana. All proteins accumulated at the cell periphery and co-localized with an endoplasmic reticulum marker. The Gc protein fusion also localized to the nucleus. N and NSm protein self-interactions and an NSm-N interaction were observed by using bimolecular fluorescence complementation. A tospovirus NSm homotypic interaction had not been reported previously.
authors: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Qld 4072, Australia.|Department of Plant Pathology, University of Kentucky, Lexington KY 40546, USA.
authors: Ralf G
authors: RG
authors: Dietzgen
authors: Department of Plant Pathology, University of Kentucky, Lexington KY 40546, USA.
authors: Kathleen M
authors: KM
authors: Martin
authors: Department of Plant Pathology, University of Kentucky, Lexington KY 40546, USA.
authors: Gavin
authors: G
authors: Anderson
authors: Department of Plant Pathology, University of Kentucky, Lexington KY 40546, USA.
authors: Michael M
authors: MM
authors: Goodin
mesh_terms: D002478:Cells, Cultured; D018506:Gene Expression Regulation, Plant; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D029069:Impatiens / Q000821:virology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D019441:Plant Epidermis / Q000166:cytology / Q000821:virology; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000235:genetics / Q000821:virology; D017865:Tospovirus / Q000145:classification* / Q000235:genetics*; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D014764:Viral Proteins