title: Development of a minigenome system for Andes virus, a New World hantavirus.
abstract: The development of reverse genetics systems for negative-stranded RNA viruses is a rapidly evolving field that has greatly advanced the study of the many different aspects of the viral life cycle. Andes virus (ANDV) is a highly pathogenic hantavirus found in South America that causes hantavirus pulmonary syndrome but to date remains poorly characterized due to the lack of a reverse genetics system for genetic manipulation. Here, we describe the first successful minigenome system for a New World hantavirus, as well as many of the obstacles that still exist in the development of such a system.
authors: Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors: Kyle S
authors: KS
authors: Brown
authors: Hideki
authors: H
authors: Ebihara
authors: Heinz
authors: H
authors: Feldmann
mesh_terms: D016679:Genome, Viral; D006476:Orthohantavirus / Q000235:genetics*; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D059386:Reverse Genetics / Q000379:methods*; D013020:South America; D014773:Virology / Q000379:methods*