title: A microfluidic flow-stretch chip for investigating blood vessel biomechanics.
abstract: This microfluidic flow-stretch chip integrates fluid shear stress (FSS) and cyclic stretch (CS), two major mechanical stimulations in cardiovascular systems, for cultured cells. The model chip can deliver FSS and CS simultaneously or independently to vascular cells to mimic the haemodynamic microenvironment of blood vessels in vivo. By imposing FSS-only, CS-only, and FSS+CS stimulation on rat mesenchymal stem cells and human umbilical vein endothelial cells, we found the alignment of the cellular stress fibers varied with cell type and the type of stimulation. The flow-stretch chip is a reliable tool for simulating the haemodynamic microenvironment.
authors: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience & Technology, 11 ZhongGuanCun BeiYiTiao, Beijing, 100190, China.
authors: Wenfu
authors: W
authors: Zheng
authors: Bo
authors: B
authors: Jiang
authors: Dong
authors: D
authors: Wang
authors: Wei
authors: W
authors: Zhang
authors: Zhuo
authors: Z
authors: Wang
authors: Xingyu
authors: X
authors: Jiang
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001808:Blood Vessels / Q000502:physiology*; D006439:Hemodynamics; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D051381:Rats; D033081:Shear Strength; D013234:Stem Cells / Q000166:cytology / Q000502:physiology