title: A polysaccharide from Sargassum fusiforme protects against immunosuppression in cyclophosphamide-treated mice.
abstract: A water-soluble polysaccharide (SFPS) isolated from Sargassum fusiforme was purified by DEAE-52 cellulose anion-exchange and Sephadex G-200 gel filtration chromatography. The high performance gel permeation chromatography (HPGPC) analysis showed that the average molecular weight (Mw) of SFPS was 299 kDa. The SFPS was composed of D-fucose, L-xylose, D-mannose and D-galactose in a molar ratio of 5.9:1.0:2.3:2.2. The results showed that SFPS stimulated proliferation and the cytokines (IL-2, IL-6 and IFN-γ) secretion of splenic lymphocytes in cyclophosphamide-induced immunosuppressed mice. SFPS markedly increased the phagocytic rates and cytokines (IL-2, IL-6 and TNF-α) secretion of peritoneal macrophages. Administration of SFPS significantly raised spleen index. It could act as an efficacious adjacent immunopotentiating therapy or an alternative means in lessening chemotherapy-induced immunosuppression, and also can be utilized as immunostimulants for food and pharmaceutical industries.
authors: Institute of Glycobiological Engineering/School of Laboratory Medicine & Life Science, Wenzhou Medical College, Wenzhou 325035, China. xmchen01@163.com
authors: Xiaoming
authors: X
authors: Chen
authors: Wenjian
authors: W
authors: Nie
authors: Sairong
authors: S
authors: Fan
authors: Junfeng
authors: J
authors: Zhang
authors: Yanxing
authors: Y
authors: Wang
authors: Jianxin
authors: J
authors: Lu
authors: Liqin
authors: L
authors: Jin
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D019610:Cytoprotection / Q000187:drug effects* / Q000276:immunology; D007108:Immune Tolerance / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008813:Mice, Inbred ICR; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*; D013154:Spleen / Q000187:drug effects
chemical_list: D007166:Immunosuppressive Agents; D011134:Polysaccharides; D003520:Cyclophosphamide