title: Global analysis of the small RNA transcriptome in different ploidies and genomic combinations of a vertebrate complex--the Squalius alburnoides.
abstract: The Squalius alburnoides complex (Steindachner) is one of the most intricate hybrid polyploid systems known in vertebrates. In this complex, the constant switch of the genome composition in consecutive generations, very frequently involving a change on the ploidy level, promotes repetitive situations of potential genomic shock. Previously in this complex, it was showed that in response to the increase in genome dosage, triploids hybrids could regulate gene expression to a diploid state. In this work we compared the small RNA profiles in the different genomic compositions interacting in the complex in order to explore the miRNA involvement in gene expression regulation of triploids. Using high-throughput arrays and sequencing technologies we were able to verify that diploid and triploid hybrids shared most of their sequences and their miRNA expression profiles were high correlated. However, an overall view indicates an up-regulation of several miRNAs in triploids and a global miRNA expression in triploids higher than the predicted from an additive model. Those results point to a participation of miRNAs in the cellular functional stability needed when the ploidy change.
authors: Centro de Biologia Ambiental, Departamento de Biologia Animal da Faculdade de Ciências, Universidade de Lisboa, Lisboa, Portugal. mainacio@fc.ul.pt
authors: Angela
authors: A
authors: Inácio
authors: Joana
authors: J
authors: Pinho
authors: Patrícia Matos
authors: PM
authors: Pereira
authors: Luca
authors: L
authors: Comai
authors: Maria Manuela
authors: MM
authors: Coelho
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003530:Cyprinidae / Q000235:genetics* / Q000502:physiology; D018628:Gene Dosage; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D016678:Genome; D023281:Genomics; D035683:MicroRNAs / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D011123:Polyploidy; D012313:RNA / Q000235:genetics*; D014158:Transcription, Genetic; D014714:Vertebrates / Q000235:genetics
chemical_list: D035683:MicroRNAs; D012313:RNA