title: PUB13, a U-box/ARM E3 ligase, regulates plant defense, cell death, and flowering time.
abstract: The ubiquitination pathway is involved in a variety of cellular processes in plant growth, development, and immune responses. However, the function of this pathway in connecting plant development and innate immunity is still largely unknown. Recently, we characterized the U-box/ARM E3 ubiquitin ligase PUB13, which regulates both immune responses and flowering time in Arabidopsis. Here, we show that the rice Spl11 gene can complement the cell death and flowering functions of PUB13 in the pub13 mutant. In addition, HFR1, which functions mainly in photomorphogenesis, was identified as one of the PUB13-interacting proteins through yeast two-hybrid screening and pull-down assays. Because the flowering phenotype of pub13 depends on photoperiod, we propose that PUB13 may regulate HFR1 to fine-tune photomorphogenesis and flowering time in Arabidopsis.
authors: Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization and Hunan Provincial Key Laboratory of Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, Hunan China.
authors: Wei
authors: W
authors: Li
authors: Liangying
authors: L
authors: Dai
authors: Guo-Liang
authors: GL
authors: Wang
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D016923:Cell Death / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000378:metabolism; D035264:Flowers / Q000166:cytology* / Q000502:physiology* / Q000528:radiation effects; D008027:Light; D009024:Morphogenesis / Q000528:radiation effects; D009687:Nuclear Proteins / Q000378:metabolism; D010935:Plant Diseases / Q000276:immunology; D030821:Plants, Genetically Modified; D011485:Protein Binding / Q000528:radiation effects; D013997:Time Factors; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D044767:Ubiquitin-Protein Ligases; C414438:At1g02340 protein, Arabidopsis; C558942:PUB13 protein, Arabidopsis