title: Comparative study on experimental autoimmune pancreatitis and its extrapancreatic involvement in mice.
abstract: OBJECTIVE
  The objective of the study was to study the relationship between autoimmune pancreatitis (AIP) and colitis in C57BL/6 interleukin 10-deficient (IL-10KO) mice and to compare the extrapancreatic involvement of AIP between IL-10KO and MRL/Mp mice that developed pancreatitis.
  
  
  METHODS
  Six-week-old female IL-10KO and MRL/Mp mice were injected intraperitoneally with polyinosinic polycytidylic acid (poly I:C) twice weekly for 8 or 12 weeks, respectively. The mice were killed, and the severity of inflammation in the pancreas, colon, liver, bile duct, and salivary gland was assessed using histological scoring systems. T-cell subsets derived from IL-10KO mice with pancreatitis were adoptively transferred into recombination activating gene 2-deficient mice.
  
  
  RESULTS
  Administration of poly I:C induced pancreatitis and accelerated the development of colitis in IL-10KO mice. Pancreatitis was characterized by specific destruction of exocrine glands and the production of various autoantibodies. Involvement of the liver and bile duct was observed in both IL-10KO and MRL/Mp mice, but sialadenitis was present only in MRL/Mp mice. Adoptive transfer of CD4(+) T cells from AIP mice induced pancreatitis in recipient mice.
  
  
  CONCLUSIONS
  Pancreatitis in IL-10KO mice resembles human type 1 AIP and is not associated with colitis. Genetic background may affect susceptibility to extrapancreatic involvement in type 1 AIP.
authors: Third Department of Internal Medicine, Kansai Medical University, Moriguchi, Japan.
authors: Masao
authors: M
authors: Yamashina
authors: Akiyoshi
authors: A
authors: Nishio
authors: Shinji
authors: S
authors: Nakayama
authors: Takashi
authors: T
authors: Okazaki
authors: Kazushige
authors: K
authors: Uchida
authors: Toshiro
authors: T
authors: Fukui
authors: Kazuichi
authors: K
authors: Okazaki
mesh_terms: D019264:Adoptive Transfer / Q000009:adverse effects; D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000637:transplantation; D002266:Carboxymethylcellulose Sodium / Q000008:administration & dosage / Q000031:analogs & derivatives; D002761:Cholangitis / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000473:pathology; D003092:Colitis / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D005088:Exocrine Glands / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D005260:Female; D006505:Hepatitis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D006801:Humans; D007369:Interferon Inducers / Q000008:administration & dosage; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D051379:Mice; D019463:Mice, Inbred MRL lpr; D018345:Mice, Knockout; D010195:Pancreatitis / Q000139:chemically induced / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D011070:Poly I-C / Q000008:administration & dosage; D011107:Polylysine / Q000008:administration & dosage / Q000031:analogs & derivatives; D012720:Severity of Illness Index; D012793:Sialadenitis / Q000139:chemically induced / Q000235:genetics / Q000276:immunology / Q000473:pathology
chemical_list: D001323:Autoantibodies; C556062:IL10 protein, mouse; D007369:Interferon Inducers; D016753:Interleukin-10; D011107:Polylysine; C019531:poly ICLC; D002266:Carboxymethylcellulose Sodium; D011070:Poly I-C