title: Ordered arrays of native chromatin molecules for high-resolution imaging and analysis.
abstract: Individual chromatin molecules contain valuable genetic and epigenetic information. To date, there have not been reliable techniques available for the controlled stretching and manipulation of individual chromatin fragments for high-resolution imaging and analysis of these molecules. We report the controlled stretching of single chromatin fragments extracted from two different cancerous cell types (M091 and HeLa) characterized through fluorescence microscopy and atomic force microscopy (AFM). Our method combines soft lithography with molecular stretching to form ordered arrays of more than 250,000 individual chromatin fragments immobilized into a beads-on-a-string structure on a solid transparent support. Using fluorescence microscopy and AFM, we verified the presence of histone proteins after the stretching and transfer process.
authors: School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, United States.
authors: Aline
authors: A
authors: Cerf
authors: Harvey C
authors: HC
authors: Tian
authors: Harold G
authors: HG
authors: Craighead
mesh_terms: D002843:Chromatin / Q000648:ultrastructure*; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D054802:Molecular Imprinting / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size
chemical_list: D002843:Chromatin