title: Secoiridoids from the stem barks of Fraxinus rhynchophylla with pancreatic lipase inhibitory activity.
abstract: Pancreatic lipase digests dietary fats by hydrolysis, which is a key enzyme for lipid absorption. Therefore, reduction of fat absorption by the inhibition of pancreatic lipase is suggested to be a therapeutic strategy for obesity. From the EtOAc-soluble fraction of the stem barks of Fraxinus rhynchophylla (Oleaceae), four secoiridoids such as ligstroside (1), oleuropein (2), 2"-hydroxyoleuropein (3) and hydroxyframoside B (4) were isolated. The inhibitory activity of these compounds on pancreatic lipase was assessed using porcine pancreatic lipase as an in vitro assay system. Compound 4 showed the strongest inhibition on pancreatic lipase, which followed by compounds 1-3. In addition, compound 4 exerted inhibitory effect on pancreatic lipase in a mixed mechanism of competitive and noncompetitive manner. Taken together, F. rhynchophylla and its constituents might be beneficial to obesity.
authors: College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea.
authors: Jong Hoon
authors: JH
authors: Ahn
authors: Eunjin
authors: E
authors: Shin
authors: Qing
authors: Q
authors: Liu
authors: Seon Beom
authors: SB
authors: Kim
authors: Kyeong-Mi
authors: KM
authors: Choi
authors: Hwan-Soo
authors: HS
authors: Yoo
authors: Bang Yeon
authors: BY
authors: Hwang
authors: Mi Kyeong
authors: MK
authors: Lee
mesh_terms: D000818:Animals; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D031661:Fraxinus / Q000737:chemistry*; D005960:Glucosides / Q000302:isolation & purification / Q000494:pharmacology; D057888:Iridoid Glucosides; D039823:Iridoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008049:Lipase / Q000037:antagonists & inhibitors*; D010179:Pancreas / Q000201:enzymology*; D024301:Plant Bark / Q000737:chemistry; D011714:Pyrans / Q000302:isolation & purification / Q000494:pharmacology; D013552:Swine
chemical_list: C586170:2''-hydroxyoleuropein; D004791:Enzyme Inhibitors; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; C561827:hydroxyframoside B; C002769:oleuropein; C445380:ligstroside; D008049:Lipase