title: Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim.
abstract: High-speed counter-current chromatography using a new liquid-liquid/solid three-phase system was used for the separation of the polyphenols corilagin and geraniin from a crude extract of Geranium wilfordii Maxim in one step. The optimized three-phase system was composed of n-hexane/ethyl acetate/methanol/acetic acid/water and to which was added 10-μm average diameter microspheres of cross-linked 12% agarose at the ratio of 0.2:10:2:1:5 and 0.1 g/mL, respectively. The purities of geraniin and corilagin were 82 and 90%, which were determined by HPLC at 280 nm. A 14.5 and 7 mg of geraniin and corilagin were purified from 160 mg crude extract with the yields of 70 and 78%, respectively.
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
authors: Dan
authors: D
authors: Liu
authors: Yan
authors: Y
authors: Ma
authors: Ming
authors: M
authors: Gu
authors: Jan-Christer
authors: JC
authors: Janson
authors: Changhai
authors: C
authors: Wang
authors: Hongbin
authors: H
authors: Xiao
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D031315:Geranium / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D059808:Polyphenols / Q000302:isolation & purification*; D012015:Reference Standards
chemical_list: D010936:Plant Extracts; D059808:Polyphenols