title: Anticancer peptide SVS-1: efficacy precedes membrane neutralization.
abstract: Anticancer peptides are polycationic amphiphiles capable of preferentially killing a wide spectrum of cancer cells relative to noncancerous cells. Their primary mode of action is an interaction with the cell membrane and subsequent activation of lytic effects; however, the exact mechanism responsible for this mode of action remains controversial. Using zeta potential analyses we demonstrate the interaction of a small anticancer peptide with membrane model systems and cancer cells. Electrostatic interactions have a pivotal role in the cell killing process, and in contrast to the antimicrobial peptides action cell death occurs without achieving full neutralization of the membrane charge.
authors: Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisbon, Portugal.
authors: Diana
authors: D
authors: Gaspar
authors: Ana Salomé
authors: AS
authors: Veiga
authors: Chomdao
authors: C
authors: Sinthuvanich
authors: Joel P
authors: JP
authors: Schneider
authors: Miguel A R B
authors: MA
authors: Castanho
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D020128:Inhibitory Concentration 50; D008567:Membranes, Artificial; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D010713:Phosphatidylcholines / Q000737:chemistry; D010718:Phosphatidylserines / Q000737:chemistry; D055672:Static Electricity; D013237:Stereoisomerism
chemical_list: D000970:Antineoplastic Agents; D008567:Membranes, Artificial; D010455:Peptides; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; C574293:SVS-1 peptide; C050294:1-palmitoyl-2-oleoylglycero-3-phosphoserine; C028694:1-palmitoyl-2-oleoylphosphatidylcholine