title: Modulation of the neurotensin solution structure in the presence of ganglioside GM1 bicelle.
abstract: Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter that shows multiple biological function in central and peripheral nervous systems. Gangliosides are glycosphingolipids, most abundant in the plasma membrane of nerve cells. Here we investigate the change of neurotensin solution structure induced by isotropic CHAPS-PC bicelles with and without ganglioside GM1 using solution state NMR spectroscopy. In aqueous solution the peptide is predominately unstructured. In the presence of bicelle overall structure of the peptide is stabilized. In CHAPS-PC bicelle neurotensin adopts 3(10) helical structure. In the presence of GM1 containing bicelle, the peptide adopts predominately 3(10) helical structures with small amount of α-helical structure. These results are consistent with the CD spectroscopic results. Neurotensin interacts better with GM1 containing bicelle than that of the CHAPS-PC bicelle. Docking studies between the Neurotensin Receptor3 (NTS3) and different NT conformations also indicated better binding of the NT conformation obtained in presence of GM1-containing bicelles.
authors: Department of Chemistry, University of Calcutta, 92, A.P.C Road, Kolkata 700009, India.
authors: Ummul Liha
authors: UL
authors: Khatun
authors: Sudipto Kishore
authors: SK
authors: Goswami
authors: Chaitali
authors: C
authors: Mukhopadhyay
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D002793:Cholic Acids / Q000737:chemistry; D002942:Circular Dichroism; D003198:Computer Simulation; D005677:G(M1) Ganglioside / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D009496:Neurotensin / Q000737:chemistry* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000737:chemistry; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D012996:Solutions / Q000737:chemistry; D000099183:Sortilin
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D002793:Cholic Acids; D005677:G(M1) Ganglioside; D008051:Lipid Bilayers; D009496:Neurotensin; D010713:Phosphatidylcholines; D012996:Solutions; D000099183:Sortilin; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate