title: Efficacy of commercial and household denture cleansers against Candida albicans adherent to acrylic denture base resin: an in vitro study.
abstract: OBJECTIVE
  To evaluate and compare the efficacy of two commercial and two household denture cleansers against Candida albicans adherent to acrylic denture base resin.
  
  
  METHODS
  Fifty specimens of acrylic denture base resin (10 × 10 × 2 mm) were fabricated and processed according to the manufacturer's instructions. Sterile acrylic resin specimens were inoculated by immersing in Sabouraud broth containing C albicans for 16 hours at 37°C in an incubator. Then the specimens were washed and immersed in denture cleansers (four groups) - Clinsodent (powder form), Fittydent (tablet form), vinegar (4% acetic acid), diluted vinegar (50% diluted with water), and water (control group) for 8 hours at room temperature. After 8 hours the acrylic resin specimens were washed, fixed with methanol, and stained with crystal violet. Candida cells adherent to the acrylic resin specimens were counted under microscope. The number of cells adherent to the test samples were compared with that adherent to the control.
  
  
  METHODS
  The data were analyzed using the independent-samples 't ' tests, analysis of variance (ANOVA), and Tukey's HSD test.
  
  
  RESULTS
  All the denture cleansers were highly effective against C albicans. The effectiveness of commercial denture cleansers was significant better than that of household denture cleansers. Fittydent fared better than Clinsodent, but the difference between the two was not statistically significant (P=.765). Vinegar was more effective than diluted vinegar (P<.05).
  
  
  CONCLUSIONS
  Within the limitations of this in vitro study, denture cleansers were found to be effective in reducing C albicans cells adhering to dentures. The commercial denture cleansers (Fittydent and Clinsodent) were more effective than household denture cleansers (vinegar and diluted vinegar).
authors: Department of Prosthodontics, Bapuji Dental College and Hospital, Davangere, India.
authors: M Nanditha
authors: MN
authors: Kumar
authors: H M
authors: HM
authors: Thippeswamy
authors: K N
authors: KN
authors: Raghavendra Swamy
authors: Anil Kumar
authors: AK
authors: Gujjari
mesh_terms: D019342:Acetic Acid / Q000627:therapeutic use; D000180:Acrylic Resins / Q000737:chemistry*; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D015169:Colony Count, Microbial; D004396:Coloring Agents; D003764:Dental Materials / Q000737:chemistry*; D003819:Denture Bases / Q000382:microbiology*; D003820:Denture Cleansers / Q000145:classification / Q000627:therapeutic use*; D005840:Gentian Violet; D006801:Humans; D008422:Materials Testing; D016877:Oxidants / Q000627:therapeutic use; D010545:Peroxides / Q000627:therapeutic use; D013696:Temperature; D013997:Time Factors; D014867:Water
chemical_list: D000180:Acrylic Resins; D004396:Coloring Agents; D003764:Dental Materials; D003820:Denture Cleansers; D016877:Oxidants; D010545:Peroxides; D014867:Water; D005840:Gentian Violet; D019342:Acetic Acid