title: Assembly of the viroplasm by viral non-structural protein Pns10 is essential for persistent infection of rice ragged stunt virus in its insect vector.
abstract: Rice ragged stunt virus (RRSV), an oryzavirus, is transmitted by brown planthopper in a persistent propagative manner. In this study, sequential infection of RRSV in the internal organs of its insect vector after ingestion of virus was investigated by immunofluorescence microscopy. RRSV was first detected in the epithelial cells of the midgut, from where it proceeded to the visceral muscles surrounding the midgut, then throughout the visceral muscles of the midgut and hindgut, and finally into the salivary glands. Viroplasms, the sites of virus replication and assembly of progeny virions, were formed in the midgut epithelium, visceral muscles and salivary glands of infected insects and contained the non-structural protein Pns10 of RRSV, which appeared to be the major constituent of the viroplasms. Viroplasm-like structures formed in non-host insect cells following expression of Pns10 in a baculovirus system, suggesting that the viroplasms observed in RRSV-infected cells were composed basically of Pns10. RNA interference induced by ingestion of dsRNA from the Pns10 gene of RRSV strongly inhibited such viroplasm formation, preventing efficient virus infection and spread in its insect vectors. These results show that Pns10 of RRSV is essential for viroplasm formation and virus replication in the vector insect.
authors: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors: Dongsheng
authors: D
authors: Jia
authors: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors: Nianmei
authors: N
authors: Guo
authors: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors: Hongyan
authors: H
authors: Chen
authors: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Fusamichi
authors: F
authors: Akita
authors: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors: Lianhui
authors: L
authors: Xie
authors: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Toshihiro
authors: T
authors: Omura
authors: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China.
authors: Taiyun
authors: T
authors: Wei
mesh_terms: D000818:Animals; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology; D004848:Epithelium / Q000378:metabolism / Q000821:virology; D007303:Insect Vectors / Q000235:genetics* / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism / Q000821:virology; D012275:Oryza / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D012087:Reoviridae / Q000235:genetics* / Q000378:metabolism; D012469:Salivary Glands / Q000378:metabolism / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism*; D014771:Virion / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication / Q000235:genetics
chemical_list: D012367:RNA, Viral; C113494:S10 protein, Rice ragged stunt virus; D017361:Viral Nonstructural Proteins