title: Large-scale in-vitro expansion of RBCs from hematopoietic stem cells.
abstract: The quest for RBCs in transfusion medicine has prompted scientists to explore the large-scale expansion of human RBCs from various sources. The successful production of RBCs in the laboratory depends on the selection of potential cell source, optimized culture, bio-physiological parameters, clinically applicable culture media that yields a scalable, contamination-free, non-reactive, non-tumorogenic, stable and functional end product. The expansion protocol considering the in vivo factors involved in homeostasis can generate a cost-effective and readily available cell source for transfusion. This review paper discusses several approaches used to expand RBCs from various sources of stem cells.
authors: Department of Stem Cells and Tissue Engineering, Frontier Life Line Pvt Ltd., Mugappair, Chennai, India.
authors: Balasundari
authors: B
authors: Ramesh
authors: Soma
authors: S
authors: Guhathakurta
mesh_terms: D020811:Biomedical Technology / Q000639:trends; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000502:physiology*; D004920:Erythropoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans