title: Preparative isolation and purification of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. by high-speed counter-current chromatography.
abstract: A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxytigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and ¹H-NMR.
authors: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
authors: Guilong
authors: G
authors: Yan
authors: Lilian
authors: L
authors: Ji
authors: Yuming
authors: Y
authors: Luo
authors: Yonghong
authors: Y
authors: Hu
mesh_terms: D000085:Acetates / Q000737:chemistry; D000440:Butanols / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D036602:Eupatorium / Q000737:chemistry*; D006586:Hexanes / Q000737:chemistry; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification*; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification*; D045788:Sesquiterpenes, Germacrane / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014867:Water / Q000737:chemistry
chemical_list: C576577:3beta-hydroxy-8beta-(4'-hydroxytigloyloxy)-costunolide; D000085:Acetates; D000440:Butanols; D004365:Drugs, Chinese Herbal; D006586:Hexanes; D007783:Lactones; D012717:Sesquiterpenes; D045788:Sesquiterpenes, Germacrane; D012997:Solvents; C571218:eupalinolide A; C571217:eupalinolide B; D014867:Water; D000431:Ethanol; C007650:ethyl acetate; D000432:Methanol