title: Dephosphorylation of Centrins by Protein Phosphatase 2C α and β.
abstract: In the present study, we identified protein phosphatases dephosphorylating centrins previously phosphorylated by protein kinase CK2. The following phosphatases known to be present in the retina were tested: PP1, PP2A, PP2B, PP2C, PP5, and alkaline phosphatase. PP2C α and β were capable of dephosphorylating P-Thr(138)-centrin1 most efficiently. PP2Cδ was inactive and the other retinal phosphatases also had much less or no effect. Similar results were observed for centrins 2 and 4. Centrin3 was not a substrate for CK2. The results suggest PP2C α and β to play a significant role in regulating the phosphorylation status of centrins in vivo.
authors: Institut für Pharmazeutische und Medizinische Chemie, Westfälische Wilhelms-Universität Münster, Hittorfstr. 58-62, D-48149 Münster, Germany.
authors: Marie-Christin
authors: MC
authors: Thissen
authors: Josef
authors: J
authors: Krieglstein
authors: Uwe
authors: U
authors: Wolfrum
authors: Susanne
authors: S
authors: Klumpp