title: [Study on purification of total organic acid and chlorogenic acid in Herba Artemisiae scopariae with macroporous adsorption resin].
abstract: OBJECTIVE
  To optimize the technological parameters of the purification process for total organic acid and chlorogenic acid in Herba Artemisiae scopariae with macroporous adsorption resin.
  
  
  METHODS
  Static and dynamic adsorption-desorption methods were adopted to choose the optimal type of resin. Then orthogonal design L9 (3(4)) and single factor experiment were used to select the optimum purification process conditions.
  
  
  RESULTS
  The optimal purification process for total organic acid and chlorogenic acid with HPD200A macroporous adsorption resin were as follows: the diameter height ratio of the resin was 1:6, the sample concentration was 1 g/mL, the absorption velocity was 1 BV/h (1 BV represented one column volume), the ratio of sample to HPD200A macroporous adsorption resin was 1.5:1 (W/W), 3 BV of water was used as purificant and 2 BV of 90% ethanol was used as eluant. Under these conditions, the purity of total organic acid and chlorogenic acid was 588.74% and 567.89%, respectively.
  
  
  CONCLUSIONS
  The established purification process for total organic acid and chlorogenic acid in Herba Artemisiae scopariae with HPD200A macroporous adsorption resin is simple and stable and can be used in industrial production.
authors: Lunan Pharmaceutical Group Co. Ltd., Linyi 276005, China. zongxisun@163.com
authors: Zong-xi
authors: ZX
authors: Sun
authors: Gui-hua
authors: GH
authors: Xu
authors: Yan-fang
authors: YF
authors: Li
authors: Xiao-hui
authors: XH
authors: Lv
mesh_terms: D000327:Adsorption; D019659:Asteraceae / Q000737:chemistry*; D002264:Carboxylic Acids / Q000737:chemistry / Q000302:isolation & purification*; D002726:Chlorogenic Acid / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D035261:Plant Components, Aerial / Q000737:chemistry; D012117:Resins, Synthetic / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D002264:Carboxylic Acids; D012117:Resins, Synthetic; D002726:Chlorogenic Acid; D000431:Ethanol