title: Changes in protein profile detected in seedlings of Caesalpinia  peltophoroides (Fabaceae) after exposure to high concentration of cadmium.
abstract: Sibipiruna (Caesalpinia peltophoroides Benth)  is a tree of the Brazilian Atlantic Forest. It is a flowering ornamental tree  widely planted throughout Brazil and indicated for restoration of degraded  areas. We examined protein profile changes in leaves of seedlings of C.  peltophoroides grown in nutrient solution under greenhouse conditions,  after exposure to cadmium (Cd; 32 mg/L). A two-dimensional gel was used to  analyze proteins expressed in response to stress 24 and 72 h after initiation  of treatment with Cd. Various protein bands were identified that were related  to stress response and/or metabolic adjustments, including proteins involved  with resistance to stress, including detoxification, degradation, antioxidant,  transport, signal transduction, photosynthesis, electron transport,  biosynthesis reactions, and transcription regulation. After 24 h of Cd  exposure, the genes of most of these proteins were upregulated. These putative  proteins were associated with resistance to stress, including heat shock  proteins, heat stress transcriptional factor and other transcriptional factors,  aquaporins, glutathione transferase and choline monooxygenase. Most of the  putative proteins observed after 72 h of exposure to Cd were downregulated.  They were mainly photosynthetic process proteins, such as NAD(P)H-quinone  oxidoreductase, photosystem I assembly, and photosystem II CP47 chlorophyll  apoprotein. There were also proteins involved with degradation, biosynthesis  and antioxidant activity, such as ATP-dependent Clp protease,  methylthioribose-1-phosphate and glutathione peroxidase 2. Based on preliminary  proteomic analysis, we conclude that proteins related to photosynthetic  activity are inhibited, decreasing plant performance under stress conditions  and that several proteins related to defense mechanisms are activated, inducing  the plant defense response.
authors: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors: L M C
authors: LM
authors: Gomes
authors: A S
authors: AS
authors: Gesteira
authors: A-A F
authors: AA
authors: de Almeida
authors: A V
authors: AV
authors: de Castro
authors: L O
authors: LO
authors: Dias
authors: C P
authors: CP
authors: Pirovani
authors: F P
authors: FP
authors: Gomes
mesh_terms: D002104:Cadmium / Q000633:toxicity*; D029911:Caesalpinia / Q000187:drug effects* / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D002104:Cadmium