title: Changes in group II phospholipase A2 gene expression in rat heart during sepsis.
abstract: BACKGROUND
  This study was undertaken to investigate alterations of group II phospholipase A2 (PLA2) gene expression and its underlying mechanism in rat heart during different phases of sepsis.
  
  
  METHODS
  Sepsis was induced by cecal ligation and puncture (CLP). Experiments were divided into three groups, control, early sepsis, and late sepsis. Early and late sepsis refers to those animals sacrificed at 9 and 18 h, respectively, after CLP. PLA2 enzyme activity, group II PLA2 protein level, messenger RNA (mRNA) abundance, transcription rate, and half-life were measured.
  
  
  RESULTS
  PLA2 activity was decreased by 29% during early sepsis but it was increased by 49% during late sepsis. Group II PLA2 protein level was decreased by 27% during early sepsis but it was increased by 35.3% during late sepsis. Group II PLA2 mRNA was decreased by 21% during early sepsis but it was increased by 141% during late sepsis. The transcription rate of group II PLA2 mRNA was reduced by 25% during early sepsis but it was elevated by 67% during late sepsis. The half-life of group II PLA2 mRNA remained unaltered during early and late phases of sepsis.
  
  
  CONCLUSIONS
  These results demonstrate that PLA2 activity, group II PLA2 protein level, the mRNA abundance, and transcription rate were concurrently underexpressed during early sepsis, while they were overexpressed during late sepsis, with no change in the degradation of gene transcript. These data indicate that the biphasic changes in group II PLA2 gene expression are regulated transcriptionally during sepsis.
authors: Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, St. Louis, Missouri, USA. mawshungliu@gmail.com
authors: Maw-Shung
authors: MS
authors: Liu
authors: Rei-Cheng
authors: RC
authors: Yang
authors: Chin
authors: C
authors: Hsu
authors: Yen-Hsu
authors: YH
authors: Chen
authors: Chia-Hsiung
authors: CH
authors: Liu
authors: Yuefang
authors: Y
authors: Zhou
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015152:Blotting, Northern; D015153:Blotting, Western; D002432:Cecum / Q000601:surgery; D005786:Gene Expression Regulation; D054501:Group II Phospholipases A2 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000201:enzymology* / Q000209:etiology / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D054501:Group II Phospholipases A2