title: Inactivation of the ybdD gene in Lactococcus lactis increases the amounts of exported proteins.
abstract: Random insertional mutagenesis performed on a Lactococcus lactis reporter strain led us to identify L. lactis ybdD as a protein-overproducing mutant. In different expression contexts, the ybdD mutant shows increased levels of exported proteins and therefore constitutes a new and attractive heterologous protein production host. This study also highlights the importance of unknown regulatory processes that play a role during protein secretion.
authors: Commensal and Probiotics-Host Interactions Laboratory, INRA, UMR1319 Micalis, Jouy-en-Josas, France.
authors: E
authors: E
authors: Morello
authors: S
authors: S
authors: Nouaille
authors: N G
authors: NG
authors: Cortes-Perez
authors: S
authors: S
authors: Blugeon
authors: L F C
authors: LF
authors: Medina
authors: V
authors: V
authors: Azevedo
authors: J J
authors: JJ
authors: Gratadoux
authors: L G
authors: LG
authors: Bermúdez-Humarán
authors: Y
authors: Y
authors: Le Loir
authors: P
authors: P
authors: Langella
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D016254:Mutagenesis, Insertional
chemical_list: D001426:Bacterial Proteins