title: Development of an MRI-visible nonviral vector for siRNA delivery targeting gastric cancer.
abstract: An antibody-directed nonviral vector, polyethylene glycol-grafted polyethylenimine functionalized with superparamagnetic iron oxide nanoparticles and a gastric cancer-associated CD44v6 single-chain variable fragment (scFv(CD44v6),-PEG-g-PEI-SPION), was constructed as a gastric cancer-targeting and magnetic resonance imaging (MRI)-visible nanocarrier for small interfering RNA (siRNA) delivery. Biophysical characterization of PEG-g-PEI-SPION and scFv(CD44v6)-PEG-g-PEI-SPION was carried out, including siRNA condensation capacity, cell viability, and transfection efficiency. Both the targeting and nontargeting nanocarriers were effective for transferring siRNA in vitro. The cellular uptake and distribution of nanoparticles complexed with siRNA was analyzed by fluorescence imaging and immunofluorescent staining. Moreover, the gastric cancer-targeting effect was verified in vivo by MRI and histology analysis. These results indicate that scFv(CD44v6)-PEG-g-PEI-SPION is a promising nonviral vector for gastric cancer gene therapy and diagnosis.
authors: Department of Gastroenterology, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors: Yinting
authors: Y
authors: Chen
authors: Weiwei
authors: W
authors: Wang
authors: Guoda
authors: G
authors: Lian
authors: Chenchen
authors: C
authors: Qian
authors: Lingyun
authors: L
authors: Wang
authors: Linjuan
authors: L
authors: Zeng
authors: Chengde
authors: C
authors: Liao
authors: Biling
authors: B
authors: Liang
authors: Bing
authors: B
authors: Huang
authors: Kaihong
authors: K
authors: Huang
authors: Xintao
authors: X
authors: Shuai
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D005260:Female; D005822:Genetic Vectors / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D006651:Histocytochemistry; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000737:chemistry / Q000493:pharmacokinetics; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D057127:Single-Chain Antibodies / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000628:therapy*; D014162:Transfection
chemical_list: D003287:Contrast Media; D004337:Drug Carriers; D034741:RNA, Small Interfering; D057127:Single-Chain Antibodies; C544016:poly(ethylene glycol)-co-poly(ethyleneimine); D011092:Polyethylene Glycols; D011094:Polyethyleneimine