title: Validated determination of primulasaponins in primula root by a high-performance-thin-layer-chromatography densitometric approach.
abstract: A novel HPTLC-densitometric method was developed for separation and quantitation of primulasaponin I and II in different matrices. HPTLC silica gel 60 F254(S), 20 cm × 10 cm, plates with ethyl acetate:water:formic acid (5:1:1 v/v) as the mobile phase were used. Densitometric determinations were performed in reflectance mode at 540 nm after derivatization with vanillin reagent. The method was validated giving rise to a dependable and high throughput procedure well suited to routine applications. Primulasaponins were quantified in the range of 150-450 ng with RSD of repeatability and intermediate precision between 0.8 and 1.4% and accuracy within the acceptance limits. The method was tested on commercial herbal medicinal preparations claiming to contain primula root extract.
authors: Dipartimento di Scienze Farmaceutiche, Università di Firenze, Via Ugo Schiff 6, I-50019 Sesto Fiorentino, Firenze, Italy. coran@unifi.it
authors: Silvia A
authors: SA
authors: Coran
authors: Stefano
authors: S
authors: Mulas
mesh_terms: D000085:Acetates / Q000737:chemistry; D001547:Benzaldehydes / Q000737:chemistry; D002138:Calibration; D002855:Chromatography, Thin Layer / Q000592:standards; D003720:Densitometry; D005561:Formates / Q000737:chemistry; D028321:Plant Preparations / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal; D031825:Primula / Q000737:chemistry*; D012015:Reference Standards; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D058428:Silica Gel / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000085:Acetates; D001547:Benzaldehydes; D005561:Formates; D028321:Plant Preparations; D012503:Saponins; D014867:Water; C030544:formic acid; D058428:Silica Gel; C007650:ethyl acetate; C100058:vanillin