title: A simple fed-batch method for transcription and insect cell-free translation.
abstract: We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.
authors: Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Aobayama 6-6-07, Sendai 980-8579, Japan.
authors: Yoko
authors: Y
authors: Sato
authors: Keishi
authors: K
authors: Aizawa
authors: Toru
authors: T
authors: Ezure
authors: Eiji
authors: E
authors: Ando
authors: Nobuyuki
authors: N
authors: Uozumi
mesh_terms: D000818:Animals; D002474:Cell-Free System; D010957:Plasmids / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera / Q000166:cytology*; D014158:Transcription, Genetic
chemical_list: D011506:Proteins