title: New fluorescent and colorimetric probe for cyanide: direct reactivity, high selectivity, and bioimaging application.
abstract: Taking advantage of the special nucleophilicity of cyanide, a new ratiometric fluorescent and colorimetric probe (Coum-1) was designed by tuning the π-conjugated bridge to affect the intramolecular charge transfer efficiency. Upon the addition of CN(-) anion, the probe displayed very large blue-shift in both fluorescence (90 nm) and absorption (90 nm) spectra, with the detection limit of 800 nM. Other anions gave nearly no interference. Furthermore, Coum-1 was successfully applied to the fluorescent microscopic imaging for the detection of CN(-) in HeLa cells.
authors: Department of Chemistry, Hubei Key Lab on Organic and Polymeric Opto-Electronic Materials, Wuhan University, Wuhan 430072, China.
authors: Xiaohong
authors: X
authors: Cheng
authors: Runli
authors: R
authors: Tang
authors: Huizhen
authors: H
authors: Jia
authors: Jun
authors: J
authors: Feng
authors: Jingui
authors: J
authors: Qin
authors: Zhen
authors: Z
authors: Li
mesh_terms: D000838:Anions; D002138:Calibration; D003124:Colorimetry / Q000379:methods*; D003486:Cyanides / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D005456:Fluorescent Dyes / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008956:Models, Chemical; D008968:Molecular Conformation; D012995:Solubility
chemical_list: D000838:Anions; D003486:Cyanides; D005456:Fluorescent Dyes; D000431:Ethanol