title: Application of solid phase microextraction on dental composite resin analysis.
abstract: A direct immersion solid phase microextraction (DI-SPME) method was developed for the analysis of dentin monomers in saliva. Dentine monomers, such as triethylene glycol dimethacrylate (TEGDMA), urethane dimethacrylate (UDMA) and 2,2-bis-[4-(2-hydroxy-3-methacryloyloxypropoxy) phenyl]-propane (Bis-GMA), have a high molecular weight and a low vapor pressure. The polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber with a medium polarity was employed for DI-SPME, and 215 nm of detection wavelength was found to be optimum in the chromatogram of HPLC measurement. The calibration range for DI-SPME was 0.30-300 μg/mL with correlation coefficients (r) greater than 0.998 for each analyte. The DI-SPME method achieved good accuracy (recovery 96.1-101.2%) and precision (2.30-8.15% CV) for both intra- and inter-day assays of quality control samples for three target compounds. Method validation was performed on standards dissolved in blank saliva, and there was no significant difference (p>0.2) between the DI-SPME method and the liquid injection method. However, the detection limit of DI-SPME was as low as 0.03, 0.27 and 0.06 μg/mL for TEGDMA, UDMA and Bis-GMA, respectively. Real sample analyses were performed on commercial dentin products after curing for the leaching measurement. In summary, DI-SPME is a more sensitive method that requires less sample pretreatment procedures to measure the resin materials leached in saliva.
authors: Department of Occupational Safety & Health, China Medical University, Taichung, Taiwan.
authors: Ven-Shing
authors: VS
authors: Wang
authors: Ta-Yuan
authors: TY
authors: Chang
authors: Chien-Chen
authors: CC
authors: Lai
authors: San-Yue
authors: SY
authors: Chen
authors: Long-Chen
authors: LC
authors: Huang
authors: Keh-Ping
authors: KP
authors: Chao
mesh_terms: D000180:Acrylic Resins / Q000032:analysis* / Q000737:chemistry*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003188:Composite Resins / Q000032:analysis* / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000032:analysis; D011092:Polyethylene Glycols / Q000032:analysis; D011109:Polymethacrylic Acids / Q000032:analysis; D011140:Polyurethanes / Q000032:analysis* / Q000737:chemistry*; D011145:Polyvinyls / Q000032:analysis; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry; D012680:Sensitivity and Specificity; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D000180:Acrylic Resins; C029012:Composite Dental Resin; D003188:Composite Resins; D004129:Dimethylpolysiloxanes; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011140:Polyurethanes; D011145:Polyvinyls; C445089:poly(dimethylsiloxane-divinylbenzene); C029824:urethane dimethacrylate luting resin; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate