title: Discrimination of type 2 diabetes mellitus corresponding to different traditional Chinese medicine syndromes based on plasma fatty acid profiles and chemometric methods.
abstract: OBJECTIVE
  Traditional Chinese medicine (TCM) has a long history and particular advantages in the diagnosis and treatment of type 2 diabetes mellitus (T2DM). Syndrome differentiation is the foundation and essence of TCM theories. The aims of the study are to discriminate T2DM corresponding to different syndromes (Qi-deficiency, Qi and Yin-deficiency and Damp heat) and discover syndrome-related biomarkers using metabolomics technology.
  
  
  METHODS
  Plasma fatty acid profiles of 85 clinical samples were established by high performance liquid chromatography (HPLC). Moreover, some of the lipid parameters, including total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL), were obtained through clinical testing methods. Orthogonal signal correction-partial least squares (OSC-PLS) and uncorrelated linear discriminant analysis (ULDA) were employed to establish two-class models for three different syndromes.
  
  
  RESULTS
  Compared with the plasma fatty acid profiles of healthy controls, the characteristic fatty acids of three TCM syndromes were discovered (p<0.01). Totally, the plasma fatty acids of T2DM were up-regulated, while significant differences existed in different syndromes. Results of ULDA indicate that the three TCM syndromes could be effectively separated by the plasma fatty acid profiles and the syndrome-related biomarkers were also screened.
  
  
  CONCLUSIONS
  The fact that three TCM syndromes can be separated indicates certain metabolic differences in different TCM syndromes of T2DM really exist and such differences can be manifested by fatty acids and lipid parameters. The results benefit modern biological interpretation of the three TCM syndromes and in a sense the diagnosis and treatment of diabetes.
authors: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors: Wenjuan
authors: W
authors: Xu
authors: Liangxiao
authors: L
authors: Zhang
authors: Yuhong
authors: Y
authors: Huang
authors: Qianxu
authors: Q
authors: Yang
authors: Hongbin
authors: H
authors: Xiao
authors: Deqin
authors: D
authors: Zhang
mesh_terms: D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005227:Fatty Acids / Q000097:blood*; D006801:Humans; D019413:Qi; D013577:Syndrome; D016710:Yin Deficiency / Q000097:blood*
chemical_list: D005227:Fatty Acids