title: Development of an automatic multi-channel ink-jet ejection chemiluminescence system and its application to the determination of horseradish peroxidase.
abstract: In this work, an automatic multi-channel ink-jet for chemiluminescence (CL) analysis was developed. The four-channel ink-jet device was controlled by a home-made circuit. Differing from the classic flow injection CL, the whole procedure for CL analysis was automatically completed on a hydrophobic glass side. CL reaction of luminal and hydrogen peroxide for the determination of horseradish peroxidase (HRP) was selected as an application to automatic CL analysis platform. All solutions delivered by different channels were precisely ejected to the same position of the glass slide for the CL analysis. The consumption of reaction solution was reduced to nanoliter level. The whole CL analysis could be completed in less than 4min, which was benefited from the prompt solution mixing in small size of droplet. The CL intensity increased linearly with HRP concentration in the range from 0.01 to 0.5μgmL(-1). The limit of detection (LOD) (S/N=3) was 0.005μgmL(-1). Finally, the automatic CL system could also be used for the detection of HRP in HRP-protein conjugates, which showed its practical application in immunoassay.
authors: Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
authors: Fengming
authors: F
authors: Chen
authors: Zhen
authors: Z
authors: Lin
authors: Yongzan
authors: Y
authors: Zheng
authors: Hulie
authors: H
authors: Zeng
authors: Hizuru
authors: H
authors: Nakajima
authors: Katsumi
authors: K
authors: Uchiyama
authors: Jin-Ming
authors: JM
authors: Lin
mesh_terms: D004867:Equipment Design; D017022:Flow Injection Analysis / Q000295:instrumentation* / Q000379:methods; D006735:Horseradish Peroxidase / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation* / Q000379:methods; D008165:Luminol / Q000737:chemistry; D008904:Miniaturization / Q000295:instrumentation* / Q000379:methods
chemical_list: D008165:Luminol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase