title: Optimum conditions of ultrasound-mediated destruction of bubble liposome for siRNA transfer in bladder cancer.
abstract: BACKGROUND
  We investigated the effectiveness of ultrasound-mediated destruction of bubble liposome (UBL) for siRNA transfer by observing reduction in the luciferase activity of human bladder tumor RT-112 cells transfected with the luciferase gene (RT-112Luc) following luciferase siRNA transfer into the cells.
  
  
  RESULTS
  siRNA was transferred to 26% of RT-112Luc cells by UBL and the luciferase activity of RT-112Luc cells was significantly suppressed by UBL using the luciferase siRNA, compared with that using nonspecific siRNA in vitro (p = 0.036). The luciferase activity of RT-112Luc tumor was suppressed by UBL using luciferase siRNA compared with that using nonspecific siRNA 2 days after the in vivo treatment.
  
  
  CONCLUSIONS
  This study showed that UBL is suitable for siRNA transfer to mammalian cells.
authors: Graduate School of Frontier Science, The University of Tokyo, Japan.
authors: Suguru
authors: S
authors: Fujisawa
authors: Hiroshige
authors: H
authors: Arakawa
authors: Ryo
authors: R
authors: Suzuki
authors: Kazuo
authors: K
authors: Maruyama
authors: Tetsuya
authors: T
authors: Kodama
authors: Masahiro
authors: M
authors: Yasunaga
authors: Yoshikatsu
authors: Y
authors: Koga
authors: Yasuhiro
authors: Y
authors: Matsumura
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D045744:Cell Line, Tumor; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008081:Liposomes; D049409:Luciferases, Firefly / Q000096:biosynthesis / Q000235:genetics; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection / Q000379:methods*; D014465:Ultrasonics; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D001426:Bacterial Proteins; D008081:Liposomes; D008164:Luminescent Proteins; D034741:RNA, Small Interfering; C054562:yellow fluorescent protein, Bacteria; D049409:Luciferases, Firefly