title: Adipose triglyceride lipase (ATGL) clone, expression pattern, and regulation by different lipid sources and lipid levels in large yellow croaker (Pseudosciaena crocea R.).
abstract: Adipose triglyceride lipase (ATGL) was recently identified as a triglyceride (TG)-specific lipase. In this study, we first obtained from large yellow croaker fish a 1,820-bp (GenBank ID: HQ916211) ATGL cDNA fragment with a 141-bp 5'UTR, a 1,485-bp open reading frame, and a 194-bp 3'UTR. The predicted fish ATGL had 494 amino acids (GenBank ID: ADY89608) and a calculated molecular weight of 55.1 kDa. ATGL was highly expressed in liver and, to a lesser degree, in heart, muscle, and abdominal fat. ATGL gene expression was high at 4.5 g and then decreased at 157.9 g and increased again at 474.2 g. The effects of lipid levels and lipid sources on ATGL expression in vivo were also investigated. A high-lipid diet decreased ATGL expression in fish significantly (P < 0.01). Fish in soybean oil group exhibited significantly lower ATGL expression than fish in the fish oil and beef tallow groups (P < 0.01). These data enhance our understanding of ATGL in fish lipid metabolism.
authors: Institute of Feed Science, Zhejiang University, No. 388 Yuhangtang Road, Hangzhou, People's Republic of China.
authors: Xinxia
authors: X
authors: Wang
authors: Yizhen
authors: Y
authors: Wang
authors: Yongjin
authors: Y
authors: Li
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D040461:Gene Components; D020869:Gene Expression Profiling; D008049:Lipase / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010473:Perciformes / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D014280:Triglycerides; D008049:Lipase