title: Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats.
abstract: OBJECTIVE
  To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS).
  
  
  METHODS
  Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3.
  
  
  RESULTS
  RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group.
  
  
  CONCLUSIONS
  N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury.
authors: Paulista Medical School, UNIFESP, Sao Paulo, Brazil.
authors: Paulo Fernandes
authors: PF
authors: Saad
authors: Karen Ruggeri
authors: KR
authors: Saad
authors: Luiz Dantas de
authors: LD
authors: Oliveira Filho
authors: Sueli Gomes
authors: SG
authors: Ferreira
authors: Marcia Kiyomi
authors: MK
authors: Koike
authors: Edna Frasson de Souza
authors: EF
authors: Montero
mesh_terms: D000111:Acetylcysteine / Q000627:therapeutic use*; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects*; D004195:Disease Models, Animal; D005440:Fluid Therapy; D020287:In Situ Nick-End Labeling; D008168:Lung / Q000166:cytology*; D055370:Lung Injury / Q000517:prevention & control; D008297:Male; D051381:Rats; D012151:Resuscitation / Q000379:methods*; D012771:Shock, Hemorrhagic / Q000188:drug therapy* / Q000473:pathology; D013997:Time Factors
chemical_list: D053148:Caspase 3; D000111:Acetylcysteine