title: A simple and sensitive HPLC-UV determination of 7-O-succinyl macrolactin A in rat plasma and urine and its application to a pharmacokinetic study.
abstract: A simple, sensitive and reproducible isocratic reversed-phase (C(18) ) high-performance liquid chromatography (HPLC) method was developed to determine 7-O-succinyl macrolactin A (SMA) in rat plasma and urine samples using UV detector set at 230 nm. Lamotrigine was used as internal standards (IS) to ensure the precision and accuracy of the method. The retention times of SMA and IS for the plasma sample were 9.2 and 4.4 min, respectively, and those for the urine samples were 7.9 and 4.3 min, respectively. The intra- and inter-day variations of the analytical responses, expressed in terms of relative standard deviation, were less than 14.9%. The accuracy, in terms of average analytical recovery, ranged from 90.4 to 119%. The lower limits of quantification of SMA in rat plasma and urine samples were 0.02 and 0.1 µg/mL, respectively. This method is applicable for the pharmacokinetic studies of SMA in rats.
authors: College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon, 420-743, South Korea.
authors: Ju Myung
authors: JM
authors: Kim
authors: Ji Won
authors: JW
authors: Jung
authors: Dong Hee
authors: DH
authors: Kim
authors: Jae Seon
authors: JS
authors: Kang
authors: Chun Gyu
authors: CG
authors: Kim
authors: Hee Eun
authors: HE
authors: Kang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D000077213:Lamotrigine; D016014:Linear Models; D018942:Macrolides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics / Q000652:urine*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014227:Triazines / Q000097:blood / Q000737:chemistry / Q000652:urine
chemical_list: C559723:7-O-succinyl macrolactin A; D018942:Macrolides; D014227:Triazines; D000077213:Lamotrigine