title: Efficacy verification and microscopic observations of an anticancer peptide, CB1a, on single lung cancer cell.
abstract: In this work, we introduce a new customized anti-lung cancer peptide, CB1a, with IC₅₀ of about 25.0 ± 1.6 μM on NCI-H460 lung cancer cells. Using a multi-cellular tumor spheroid (MCTS) model, results show that CB1a is potent in preventing the growth of lung cancer tumor-like growths in vitro. Additionally, atomic force microscopy (AFM) was used to examine cell surface damage of a single cancer. The mechanism for cell death under CB1a toxicity was verified as being largely due to cell surface damage. Moreover, with a treatment dosage of CB1a at 25 μM, Young's module (E) shows that the elasticity and stiffness of cancer cell decreased with time such that the interaction time for a 50% reduction of E (IT₅₀) was about 7.0min. This new single-cell toxicity investigation using IT₅₀ under AFM assay can be used to separately verify drug efficacy in support of the traditional IC₅₀ measurement in bulk solution. These results could be of special interest to researchers engaged in new drug development.
authors: National Nano Device Laboratories, Nano Biomedical Group, Hsinchu, Taiwan.
authors: Feng-Sheng
authors: FS
authors: Kao
authors: Yun-Ru
authors: YR
authors: Pan
authors: Ray-Quen
authors: RQ
authors: Hsu
authors: Hueih-Min
authors: HM
authors: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry* / Q000494:pharmacology; D008175:Lung Neoplasms / Q000188:drug therapy*; D018625:Microscopy, Atomic Force; D010446:Peptide Fragments / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D019476:Insect Proteins; D010446:Peptide Fragments; C115150:cecropin B1 protein, insect