title: Role of curcuminoids in ameliorating oxidative modification in β-thalassemia/Hb E plasma proteome.
abstract: Thalassemic patients often exhibit high levels of oxidative stress and iron overload, which can lead to hazardous complications. Curcuminoids, extracted from the spice turmeric, are known to have antioxidant and iron-chelating properties and have been proposed as a potential upstream therapy of thalassemia. Here we have applied proteomic techniques to study the protein profile and oxidative damage in the plasma of β-thalassemia/Hb E patients before and after treatment with curcuminoids. In this study, 10 β-thalassemia/Hb E patients were treated with 500 mg curcuminoids daily for 12 months. The plasma protein profile and protein carbonyl content were determined at baseline, 6 and 12 months using two-dimensional fluorescence difference gel electrophoresis and carbonyl immunoblotting, respectively. Other hematological, clinical, and biochemical parameters were also analyzed. Twenty-six spots, identified as coagulation factors and proteins involved in iron homeostasis, showed significantly decreased intensity in thalassemic plasma, compared to those of normal subjects. Treatment with curcuminoids up-regulated the plasma levels of these proteins and reduced their oxidative damage. Serum non-transferrin bound iron, platelet factor-3 like activity, oxidative stress parameters and antioxidant enzymes were also improved after curcuminoids treatment. This study is the first proteomic study of plasma in the thalassemic state and also shows the ameliorating role of curcuminoids towards oxidative stress and iron overload in the plasma proteome.
authors: Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand.
authors: Churat
authors: C
authors: Weeraphan
authors: Chantragan
authors: C
authors: Srisomsap
authors: Daranee
authors: D
authors: Chokchaichamnankit
authors: Pantipa
authors: P
authors: Subhasitanont
authors: Suneerat
authors: S
authors: Hatairaktham
authors: Ratiya
authors: R
authors: Charoensakdi
authors: Narumol
authors: N
authors: Panichkul
authors: Noppadol
authors: N
authors: Siritanaratkul
authors: Suthat
authors: S
authors: Fucharoen
authors: Jisnuson
authors: J
authors: Svasti
authors: Ruchaneekorn W
authors: RW
authors: Kalpravidh
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000494:pharmacology; D030024:Curcuma / Q000737:chemistry; D019587:Dietary Supplements; D005260:Female; D006446:Hemoglobin E; D006801:Humans; D007502:Iron Chelating Agents / Q000737:chemistry; D019190:Iron Overload / Q000188:drug therapy; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000493:pharmacokinetics*; D050050:Protein Carbonylation; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D014168:Transferrin / Q000032:analysis / Q000378:metabolism; D015854:Up-Regulation; D055815:Young Adult; D017086:beta-Thalassemia / Q000188:drug therapy*
chemical_list: D000975:Antioxidants; D007502:Iron Chelating Agents; D010936:Plant Extracts; D020543:Proteome; D014168:Transferrin; C012596:turmeric extract; D006446:Hemoglobin E