title: The nucleoprotein is responsible for intracerebral pathogenicity of A/duck/Mongolia/47/2001 (H7N1) in chicks.
abstract: Avian influenza viruses A/duck/Mongolia/47/2001 (H7N1) (47/01) and A/duck/Mongolia/867/2002 (H7N1) (867/02) were defined as low-pathogenic avian influenza viruses (LPAIVs) using an intravenous pathogenicity test in chickens. On the other hand, the intracerebral pathogenicity indices of 47/01 and 867/02 were 1.30 and 0.00, respectively. A series of reassortant viruses were generated between 47/01 and 867/02, and their intracerebral pathogenicity was compared in one-day-old chicks to identify the protein(s) responsible for the intracerebral pathogenicity of 47/01. The results indicate that the amino acids at positions 50 and 98 of the nucleoprotein are related to the pathogenicity of 47/01 in chicks by intracerebral inoculation. A significant association was found between mortality of the chicks inoculated intracerebrally with 47/01 and virus replication in the lungs and/or brain. These results indicate that the NP of avian influenza viruses may be responsible for intracerebral pathogenicity in the host.
authors: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18-jo Nishi-9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan.
authors: Norikazu
authors: N
authors: Isoda
authors: Yoshimi
authors: Y
authors: Tsuda
authors: Shingo
authors: S
authors: Asakura
authors: Masatoshi
authors: M
authors: Okamatsu
authors: Yoshihiro
authors: Y
authors: Sakoda
authors: Hiroshi
authors: H
authors: Kida
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000473:pathology / Q000821:virology; D002642:Chick Embryo; D002645:Chickens; D004372:Ducks; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D057916:Influenza A Virus, H7N1 Subtype / Q000378:metabolism* / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000473:pathology / Q000821:virology*; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016865:Reassortant Viruses
chemical_list: D009698:Nucleoproteins; D012367:RNA, Viral