title: The mechanism of antibacterial activity of tetrandrine against Staphylococcus aureus.
abstract: Tetrandrine (TET) is a bis-benzylisoquinoline alkaloid derived from the radix of Stephania tetrandra S. Moore. TET performs a wide spectrum of biological activities. The radix of S. tetrandrae has been used traditionally in Asia, including Korea, to treat congestive circulatory disorders and inflammatory diseases. The aim of this study was to examine the mechanism of antibacterial activity of tetrandrine against Staphylococcus aureus. The mechanism was investigated by studying the effects of TET in combination with detergent or membrane potential un-couplers. In addition, the direct involvement of peptidoglycan (PGN) was assessed in titration assays. TET activity against S. aureus was 125-250 μg/mL, and the minimum inhibitory concentration (MIC) of the two reference strains was 250 μg/mL. The OD(600) of each suspension treated with a combination of ethylenediaminetetraacetic acid (EDTA), tris(hydroxymethyl) aminomethane (TRIS), and Triton X-100 (TX) with TET (0.25×MIC) had been reduced from 43% to 96%. Additional structure-function studies on the antibacterial activity of TET in combination with other agents may lead to the discovery of more effective antibacterial agents.
authors: Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, Eumsung, Chungbuk, Republic of Korea.
authors: Young-Seob
authors: YS
authors: Lee
authors: Sin-Hee
authors: SH
authors: Han
authors: Su-Hwan
authors: SH
authors: Lee
authors: Young-Guk
authors: YG
authors: Kim
authors: Chung-Berm
authors: CB
authors: Park
authors: Ok-Hwa
authors: OH
authors: Kang
authors: Joon-Ho
authors: JH
authors: Keum
authors: Sung-Bae
authors: SB
authors: Kim
authors: Su-Hyun
authors: SH
authors: Mun
authors: Yun-Soo
authors: YS
authors: Seo
authors: Noh-Yil
authors: NY
authors: Myung
authors: Dong-Yeul
authors: DY
authors: Kwon
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D044182:Benzylisoquinolines / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D004492:Edetic Acid / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D017830:Octoxynol / Q000737:chemistry; D010457:Peptidoglycan / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000472:pathogenicity; D031609:Stephania tetrandra / Q000737:chemistry; D014325:Tromethamine / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D044182:Benzylisoquinolines; D004791:Enzyme Inhibitors; D010457:Peptidoglycan; D010936:Plant Extracts; D014325:Tromethamine; C009438:tetrandrine; D017830:Octoxynol; D004492:Edetic Acid; D000251:Adenosine Triphosphatases