title: Annexin A9 is a periplakin interacting partner in membrane-targeted cytoskeletal linker protein complexes.
abstract: Periplakin regulates keratin organisation and participates in the assembly of epidermal cornified envelopes. A proteomic approach identified annexin A9 as a novel interacting partner for periplakin N-terminus. The presence of annexin A9 in complexes with periplakin was confirmed by immunoblotting of proteins immunoprecipitated by anti-HA or anti-annexin A9 antibodies. Both endogenous and GFP-tagged annexin A9 co-localise with endogenous periplakin and transfected periplakin N-terminus at MCF-7 cell borders and aggregate after Okadaic acid treatment. Annexin A9 and periplakin co-localise in the epidermis and annexin A9 is up-regulated in differentiating keratinocytes, but the epidermal annexin A9 expression does not require periplakin.
authors: School of Biological and Biomedical Sciences, Durham University, Durham, United Kingdom.
authors: Veronika
authors: V
authors: Boczonadi
authors: Arto
authors: A
authors: Määttä
mesh_terms: D000818:Animals; D017302:Annexins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D061986:MCF-7 Cells; D051379:Mice; D046912:Multiprotein Complexes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D019319:Okadaic Acid / Q000494:pharmacology; D051179:Plakins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: C114643:ANXA9 protein, human; D017302:Annexins; C496015:Anxa9 protein, mouse; D003598:Cytoskeletal Proteins; D046912:Multiprotein Complexes; C491674:PPL protein, human; D051179:Plakins; C110037:Ppl protein, mouse; D011994:Recombinant Proteins; D019319:Okadaic Acid