title: Interplay between protein-modified surface and functional response of osteoblasts.
abstract: The objective of the study is to elucidate the interplay between fibronectin-metal hybrid surfaces and osteoblast function. The practical relevance is that a significant initial step in the process of prosthetic integration within a physiological system is the rapid adsorption of proteins, including fibronectin, on the surface of biomedical device. Here, we compare and contrast the cell-substrate interactions on bare and protein-modified surfaces. The protein adsorption on the surface was beneficial in favorably modulating biological functions including cell attachment, proliferation, and viability. Additionally, immunofluorescence studies demonstrated stronger vinculin signals associated with actin stress fibers in the outer regions of the cells and cellular extensions on protein-adsorbed surfaces. These results support the hypothesis that protein adsorption on artificial biomedical devices can promote bioactivity and regulate biological functions.
authors: BiCenter for Structural and Functional Materials, University of Louisiana at Lafayette, Lafayette, Louisiana 70504, USA.
authors: P
authors: P
authors: Chaudhary
authors: T C
authors: TC
authors: Pesacreta
authors: R D K
authors: RD
authors: Misra
mesh_terms: D016475:3T3 Cells; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D013193:Stainless Steel / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D013193:Stainless Steel