title: Suppressive effects of coumarins from Mammea siamensis on inducible nitric oxide synthase expression in RAW264.7 cells.
abstract: A methanol extract of the flowers of Mammea siamensis (Calophyllaceae) was found to inhibit nitric oxide (NO) production in lipopolysaccharide-activated RAW264.7 cells. From the extract, two new geranylated coumarins, mammeasins A (1) and B (2), were isolated together with 17 known compounds including 15 coumarins. The structures of 1 and 2 were determined on the basis of their spectroscopic properties as well as of their chemical evidence. Among the isolates, 1 (IC(50) = 1.8 μM), 2 (6.4 μM), surangins B (3, 5.0 μM), C (4, 6.8 μM), and D (5, 6.2 μM), kayeassamins E (7, 6.1 μM), F (8, 6.0 μM), and G (9, 0.8 μM), mammea A/AD (11, 1.3 μM), and mammea E/BB (16, 7.9 μM) showed NO production inhibitory activity. Compounds 1, 9, and 11 were found to inhibit induction of inducible nitric oxide synthase (iNOS). With regard to mechanism of action of these active constituents (1, 9, and 11), suppression of STAT1 activation is suggested to be mainly involved in their suppression of iNOS induction.
authors: Pharmaceutical Research and Technology Institute, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka 577-8502, Japan.
authors: Toshio
authors: T
authors: Morikawa
authors: Mayumi
authors: M
authors: Sueyoshi
authors: Saowanee
authors: S
authors: Chaipech
authors: Hisashi
authors: H
authors: Matsuda
authors: Yukiko
authors: Y
authors: Nomura
authors: Mikuko
authors: M
authors: Yabe
authors: Tomoko
authors: T
authors: Matsumoto
authors: Kiyofumi
authors: K
authors: Ninomiya
authors: Masayuki
authors: M
authors: Yoshikawa
authors: Yutana
authors: Y
authors: Pongpiriyadacha
authors: Takao
authors: T
authors: Hayakawa
authors: Osamu
authors: O
authors: Muraoka
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D031252:Mammea / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism; D013237:Stereoisomerism; D013329:Structure-Activity Relationship
chemical_list: D003374:Coumarins; D004791:Enzyme Inhibitors; D052247:Nitric Oxide Synthase Type II