title: Effect of Huxin Formula on reverse cholesterol transport in ApoE-gene knockout mice.
abstract: OBJECTIVE
  To observe the effect of Huxin Formula on expressions of the chief reverse cholesterol transport (RCT) associated genes, caveolin-1 and scavenger receptor-BI (SR-BI) in ApoE-gene knockout [ApoE (-/-)] mice.
  
  
  METHODS
  Thirty ApoE (-/-) mice of 4-6 weeks old were randomly divided into three groups (A-C). After being fed with high-fat diet for 16 weeks, they were treated with HXF (1 mL/100 g), pravachol (0.3 mg/100 g), and saline in equal volume respectively for 16 weeks successively; in addition, a blank group was set up with 10 C57BL/6J mice of 6-week old received 16-week high-fat feeding and saline treatment. Animals were sacrificed at the termination of the experiment, their paraffin sections of aortic tissue were used to measure the size of plaque, expressions of cavolin-1 and SR-BI were detected by immunological histochemical method.
  
  
  RESULTS
  As compared with the blank group, levels of caveolin-1 and SR-BI were increased in Groups A and B (P<0.01); but the increase in Group A was more significant than that in Group B (P<0.05). The plaque/aorta area ratio decreased significantly in Groups A and B, but showed insignificant difference between the two groups.
  
  
  CONCLUSIONS
  HXF could obviously increase the expressions of RCT associated genes, caveolin-1 and SR-BI, promote the RCT process, so as to reduce the formation of aorta atherosclerotic plaque in ApoE (-/-) mice.
authors: The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou (510120), China. drjiangwei@163.com
authors: Wei
authors: W
authors: Jiang
authors: Song
authors: S
authors: Li
authors: Wei
authors: W
authors: Mao
authors: Guang
authors: G
authors: Yang
authors: Xin-mei
authors: XM
authors: Li
authors: Guang-juan
authors: GJ
authors: Zheng
authors: Huan-lin
authors: HL
authors: Wu
authors: Xin-min
authors: XM
authors: Ruan
authors: Ke-ji
authors: KJ
authors: Chen
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000473:pathology; D001057:Apolipoproteins E / Q000172:deficiency* / Q000235:genetics*; D050197:Atherosclerosis / Q000473:pathology; D001692:Biological Transport / Q000187:drug effects; D051242:Caveolin 1 / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D058226:Plaque, Atherosclerotic / Q000473:pathology; D051116:Receptors, Scavenger / Q000378:metabolism
chemical_list: D001057:Apolipoproteins E; D051242:Caveolin 1; D004365:Drugs, Chinese Herbal; D051116:Receptors, Scavenger; C578964:huxin; D002784:Cholesterol