title: Downregulating PRL-3 inhibit migration and invasion of lung cancer cell via RhoA and mDia1.
abstract: OBJECTIVE
  The overexpression of phosphatase of regenerating liver-3 (PRL-3) affects the migration and invasion of epithelial cells. Little is known about the substrates or pathways that PRL-3 interacts with.
  
  
  METHODS
  We studied the action and the mechanism of PRL-3 in migration and invasion of lung cancer. We detected the expression of PRL-3 in lung cancer cell lines and normal human bronchial epithelial cell.
  
  
  RESULTS
  We found that PRL-3 expression was high in lung cancer cells. Knockdown of PRL-3 by siRNA inhibited cell migration and invasion and reorganizated the cytoskeleton. Furthermore, blocking PRL-3 decreased RhoA activity and mDia1 expression. Blocking RhoA or mDia1 showed the similar changes of cytoskeleton and suppression of migration and invasion as inhibiting PRL-3 expression. Blocking RhoA inhibited the expression of mDia1.
  
  
  CONCLUSIONS
  These data indicate that downregulating PRL-3 inhibit cell migration and invasion by inactivating RhoA to downregulate mDia1 to reorganizate cytoskeleton of lung cancer cells.
authors: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang; PR China.
authors: Ming
authors: M
authors: Jian
authors: Liu
authors: L
authors: Nan
authors: Jiang
authors: J
authors: Guocheng
authors: Zhang
authors: Z
authors: Qingfu
authors: Qiu
authors: Q
authors: Xueshan
authors: Wang
authors: W
authors: Enhua
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000230:Adenocarcinoma / Q000201:enzymology*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000201:enzymology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism; D018450:Disease Progression; D015536:Down-Regulation; D005455:Fluorescent Antibody Technique; D000080865:Formins; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020742:rhoA GTP-Binding Protein / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C108962:DIAPH1 protein, human; D000080865:Formins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; C484730:PTP4A3 protein, human; D017027:Protein Tyrosine Phosphatases; D020742:rhoA GTP-Binding Protein