title: Evaluation of cytotoxic compounds in different organs of the sea bream Sarpa salpa as related to phytoplankton consumption: an in vitro study in human liver cell lines HepG2 and WRL68.
abstract: The present study was aimed to assess the cytotoxic effects of not-yet identified compounds present in organ extracts of Sarpa salpa, collected in autumn, the period with a peak in health problems. In addition, we studied the cytotoxicity of extracts of epiphytes found in the stomach content of S. salpa collected in summer and of epiphytes collected from the sea in the Sfax area at the end of spring. We tested these fractions in two human hepatic cell lines: HepG2 and WRL68. We observed a significant loss of viable cells when HepG2 cells were exposed for 72 h to acetone extracts of livers of S. salpa at a concentration of 2.5 mg/ml protein. Proteins extracted from brain or muscle did not significantly induce cell death at the studied concentrations (≤10 mg/ml). There was a significant loss of viable cells when treated with liver extract of S. salpa dissolved in DMSO. Extracts of epiphytes collected in late spring showed a cytotoxic effect in a concentration-dependent manner. Moreover, we observed a significantly decreased cell viability of HepG2 at a dilution (1/40) of epiphyte extracts from stomach contents of two fish we had collected. The cytotoxic effect of the observed epiphyte extracts confirms the transfer of toxins originating from toxic dinoflagellates which live in epiphyte on the Posidonia oceanica leaves to fish organs by grazing. Hence, the liver of this fish can cause a threat to human health and consumption should for this reason be dissuaded.
authors: Animal Ecophysiology Laboratory, Sciences Faculty of Sfax, Department of Life Sciences, BP 1171, 3000, Sfax, Tunisia. khaledbra3@gmail.com
authors: Khaled
authors: K
authors: Bellassoued
authors: Asma
authors: A
authors: Hamza
authors: Jos
authors: J
authors: Van Pelt
authors: Abdelfattah
authors: A
authors: Elfeki
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D004838:Epiphyses / Q000737:chemistry; D005504:Food Analysis; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000737:chemistry; D010839:Phytoplankton / Q000378:metabolism; D021541:Sea Bream; D014020:Tissue Extracts / Q000737:chemistry / Q000633:toxicity*
chemical_list: D014020:Tissue Extracts