title: Oleanolic acid potentiates the antitumor activity of 5-fluorouracil             in pancreatic cancer cells.
abstract: The antitumor activity of oleanolic (OA) has attracted attention due to             its marked antitumor effects and pharmacological safety. In the present study,             the effects of the combination of OA and 5-fluorouracil (5-FU) on Panc-28 human             pancreatic cells were studied. The results showed that combined use of OA and             5-FU synergistically potentiated cell death effects on Panc-28 cells, and the             pro-apoptotic effects were also increased. Further study revealed that the combined             treatment could enhance mitochondrial depolarization, lysosomal membrane permeabilization             (LMP) and leakage of cathepin D, while the release of cytochrome C did not display             significant changes. The expression of apoptosis related proteins was also affected             in cells treated with the combination of OA and 5-FU, including activation of             caspases-3 and the expression of Bcl-2/Bax, survivin and NF-κB. Our results provide             evidence that combination of OA and 5-FU may serve as a novel strategy for the             treatment of pancreatic cancer.
authors: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors: Jianteng
authors: J
authors: Wei
authors: Haizhou
authors: H
authors: Liu
authors: Ming
authors: M
authors: Liu
authors: Ning
authors: N
authors: Wu
authors: Jin
authors: J
authors: Zhao
authors: Lin
authors: L
authors: Xiao
authors: Lijun
authors: L
authors: Han
authors: Edward
authors: E
authors: Chu
authors: Xiukun
authors: X
authors: Lin
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002402:Cathepsin D / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D004354:Drug Screening Assays, Antitumor; D005472:Fluorouracil / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism; D007425:Intracellular Membranes / Q000187:drug effects; D008247:Lysosomes / Q000187:drug effects; D016328:NF-kappa B / Q000378:metabolism; D009828:Oleanolic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D010539:Permeability; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D000077022:Survivin; D014407:Tumor Cells, Cultured / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D000077022:Survivin; D051028:bcl-2-Associated X Protein; D009828:Oleanolic Acid; D045304:Cytochromes c; D053148:Caspase 3; C533922:CTSD protein, human; D002402:Cathepsin D; D005472:Fluorouracil