title: Chikungunya virus nsP3 blocks stress granule assembly by recruitment of G3BP into cytoplasmic foci.
abstract: Chikungunya virus nonstructural protein nsP3 has an essential but unknown role in alphavirus replication and interacts with Ras-GAP SH3 domain-binding protein (G3BP). Here we describe the first known function of nsP3, to inhibit stress granule assembly by recruiting G3BP into cytoplasmic foci. A conserved SH3 domain-binding motif in nsP3 is essential for both nsP3-G3BP interactions and viral RNA replication. This study reveals a novel role for nsP3 as a regulator of the cellular stress response.
authors: Laboratory of Virology, Wageningen University, Wageningen, The Netherlands.
authors: Jelke J
authors: JJ
authors: Fros
authors: Natalia E
authors: NE
authors: Domeradzka
authors: Jim
authors: J
authors: Baggen
authors: Corinne
authors: C
authors: Geertsema
authors: Jacky
authors: J
authors: Flipse
authors: Just M
authors: JM
authors: Vlak
authors: Gorben P
authors: GP
authors: Pijlman
mesh_terms: D018354:Alphavirus Infections / Q000378:metabolism / Q000821:virology*; D020816:Amino Acid Motifs; D000818:Animals; D065632:Chikungunya Fever; D002646:Chikungunya virus / Q000235:genetics*; D002522:Chlorocebus aethiops; D017124:Conserved Sequence; D003593:Cytoplasm / Q000378:metabolism*; D017353:Gene Deletion; D008957:Models, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012367:RNA, Viral / Q000378:metabolism; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics*; D014779:Virus Replication; D018909:src Homology Domains
chemical_list: D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; C089383:nsp3 protein, alphavirus