title: Improved gene transfer into bladder cancer cells using adenovirus vector containing RGD motif.
abstract: BACKGROUND
  The transduction efficacy of adenovirus serotype 5 (Ad5) vector in high-grade human bladder cancer cells is generally extremely low due to the non-expression of coxsackie and adenoviral receptor (CAR). We investigated whether fiber-modified adenovirus vector containing an RGD motif in the HI loop of the adenovirus fiber knob could increase the transduction efficiency of Ad5 into human bladder cancer cells in vitro.
  
  
  METHODS
  We examined the expressions of CAR, and of α(v), β(3) and β(5) integrin, and the transduction efficacy of fiber-modified adenovirus vector in four human bladder cancer cell lines (TCC-SUP, 253J, T24 and KK47).
  
  
  RESULTS
  The expression of CAR was lower and those of α(v) and β(3) integrin were higher in four human cancer cell lines compared with the control cell line, KK47. The transduction efficacy of fiber-modified adenovirus vector increased by 20- to 470-fold compared with Ad5.
  
  
  CONCLUSIONS
  Fiber-modified adenovirus vector may be useful in order to establish new effective gene therapy strategies for the treatment of high-grade human bladder cancer.
authors: Laboratory of Cell and Gene Therapy, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Nishinomiya, Japan.
authors: Kazuhiro
authors: K
authors: Hiwasa
authors: Hisao
authors: H
authors: Nagaya
authors: Shuji
authors: S
authors: Terao
authors: Bishnu
authors: B
authors: Acharya
authors: Katsuyuki
authors: K
authors: Hamada
authors: Hiroyuki
authors: H
authors: Mizuguchi
authors: Akinobu
authors: A
authors: Gotoh
mesh_terms: D000256:Adenoviridae; D001483:Base Sequence; D045744:Cell Line, Tumor; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D017931:DNA Primers; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D016023:Integrins / Q000235:genetics; D009842:Oligopeptides / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011991:Receptors, Virus / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014161:Transduction, Genetic / Q000379:methods*; D001749:Urinary Bladder Neoplasms / Q000628:therapy*
chemical_list: C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D017931:DNA Primers; D016023:Integrins; D009842:Oligopeptides; D012333:RNA, Messenger; D011991:Receptors, Virus; C047981:arginyl-glycyl-aspartic acid