title: In vitro expression of vascular endothelial growth factor and its receptors by placental macrophages.
abstract: The expression of VEGF and membrane-bound and soluble forms of the VEGF-R1 receptor in cultured placental macrophages (trimesters I and III of pregnancy) was studied by flow cytometry, cytometric bead array, and ELISA. Nearly all population of placental macrophages (98%) was capable of producing VEGF during the early and late gestational periods. However, the expression of cellular VEGF-R1 varied from 3.4 to 92%. VEGF secretion was relatively low in the first and third trimesters (0.5 and 1.1 pg/10(5) cells, respectively). Cultured placental macrophages produced soluble receptor sVEGF-R1 in the first and third trimesters (86.4 and 36.4 pg/10(5) cells, respectively). Stimulation with LPS was followed by a 4-fold increase in sVEGF-R1 secretion. Our results indicate that placental macrophages are involved in the autocrine and paracrine regulation in chorionic villi. The data suggest that these cells have a physiological and pathogenetic role in gestation.
authors: D. O. Ott Institute of Obstetrics and Gynecology, Northwestern Division of the Russian Academy of Medical Sciences, St. Petersburg, Russia. ovpavlov@hotmail.com
authors: O V
authors: OV
authors: Pavlov
authors: T S
authors: TS
authors: Sheveleva
authors: S A
authors: SA
authors: Selkov
mesh_terms: D002478:Cells, Cultured; D002824:Chorionic Villi; D005260:Female; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D010920:Placenta / Q000166:cytology* / Q000276:immunology; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000096:biosynthesis*; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis*; D042442:Vascular Endothelial Growth Factors / Q000096:biosynthesis*
chemical_list: D042442:Vascular Endothelial Growth Factors; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2