title: The involvement of nitric oxide in the physiopathology of hepatoportal sclerosis.
abstract: OBJECTIVE
  Hepatoportal sclerosis (HPS) is a clinical syndrome of unspecified etiology depicted by enlarged spleen and portal hypertension in the lack of other chronic liver disease findings, hematological disorders or any infectious disease in the liver. Nitric oxide (NO) molecule has many important functions in human body including phagocytosis in macrophages, neural transmission and endothelial relaxation. Although there is no data in literature that depicts the role of NO in HPS pathogenesis, this study was conducted in order to evaluate the potential role of NO in patients with HPS.
  
  
  METHODS
  The study participants included 24 HPS patients and 20 healthy controls. The median age of HPS and control patients was 41.2 ± 13.9 and 46.5 ± 12.4 years, respectively. NO was predicted as nitric oxide metabolites (NOx) by Griess reaction after transformation of nitrate to nitrite by nitrate reductase using the commercially obtainable Nitric Oxide Assay Kit.
  
  
  RESULTS
  Serum NOx levels were 2.69 ± 2.98 μmol/L and 0.85 ± 1.05 μmol/L for the HPS patients and controls, respectively. Serum NO levels were significantly higher in patients with HPS compared to the control group (p<0.001). ROC curve analysis suggested that the optimum NOx cut-off point for HPS was 1.305 with sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 83.3%, 90 %, 90.9 %, and 81.8% respectively.
  
  
  CONCLUSIONS
  Circulating NO concentration was notably higher in patients with HPS in comparison to the control group. Our study verified that an elevated level of NO might have a role in the pathogenesis of HPS.
authors: MD, Department of Gastroenterology Ankara Numune Education and Research Hospital, Ankara, Turkey. purnakt@yahoo.com
authors: Tugrul
authors: T
authors: Purnak
authors: Yavuz
authors: Y
authors: Beyazit
authors: Mehmet
authors: M
authors: Ibis
authors: Seyfettin
authors: S
authors: Koklu
authors: Cumali
authors: C
authors: Efe
authors: Ersan
authors: E
authors: Ozaslan
authors: Aydin
authors: A
authors: Ciftci
authors: Ilyas
authors: I
authors: Tenlik
mesh_terms: D000015:Abnormalities, Multiple / Q000097:blood* / Q000503:physiopathology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D006975:Hypertension, Portal / Q000097:blood* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood* / Q000502:physiology; D012372:ROC Curve; D012598:Sclerosis; D013163:Splenomegaly / Q000097:blood* / Q000503:physiopathology; D013577:Syndrome; D055815:Young Adult; D005723:gamma-Glutamyltransferase / Q000097:blood
chemical_list: D009569:Nitric Oxide; D005723:gamma-Glutamyltransferase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase