title: Transgenic RNA interference (RNAi)-derived field resistance to cassava brown streak disease.
abstract: Cassava brown streak disease (CBSD), caused by the Ipomoviruses Cassava brown streak virus (CBSV) and Ugandan Cassava brown streak virus (UCBSV), is considered to be an imminent threat to food security in tropical Africa. Cassava plants were transgenically modified to generate small interfering RNAs (siRNAs) from truncated full-length (894-bp) and N-terminal (402-bp) portions of the UCBSV coat protein (ΔCP) sequence. Seven siRNA-producing lines from each gene construct were tested under confined field trials at Namulonge, Uganda. All nontransgenic control plants (n = 60) developed CBSD symptoms on aerial tissues by 6 months after planting, whereas plants transgenic for the full-length ΔCP sequence showed a 3-month delay in disease development, with 98% of clonal replicates within line 718-001 remaining symptom free over the 11-month trial. Reverse transcriptase-polymerase chain reaction (RT-PCR) diagnostics indicated the presence of UCBSV within the leaves of 57% of the nontransgenic controls, but in only two of 413 plants tested (0.5%) across the 14 transgenic lines. All transgenic plants showing CBSD were PCR positive for the presence of CBSV, except for line 781-001, in which 93% of plants were confirmed to be free of both pathogens. At harvest, 90% of storage roots from nontransgenic plants were severely affected by CBSD-induced necrosis. However, transgenic lines 718-005 and 718-001 showed significant suppression of disease, with 95% of roots from the latter line remaining free from necrosis and RT-PCR negative for the presence of both viral pathogens. Cross-protection against CBSV by siRNAs generated from the full-length UCBSV ΔCP confirms a previous report in tobacco. The information presented provides proof of principle for the control of CBSD by RNA interference-mediated technology, and progress towards the potential control of this damaging disease.
authors: National Crops Resources Research Institute, Namulonge, Kampala, Uganda.
authors: Emmanuel
authors: E
authors: Ogwok
authors: John
authors: J
authors: Odipio
authors: Mark
authors: M
authors: Halsey
authors: Eliana
authors: E
authors: Gaitán-Solís
authors: Anton
authors: A
authors: Bua
authors: Nigel J
authors: NJ
authors: Taylor
authors: Claude M
authors: CM
authors: Fauquet
authors: Titus
authors: T
authors: Alicai
mesh_terms: D000383:Agriculture; D000818:Animals; D060467:Disease Resistance / Q000276:immunology*; D018506:Gene Expression Regulation, Plant; D006430:Hemiptera / Q000502:physiology; D002365:Manihot / Q000235:genetics* / Q000276:immunology / Q000469:parasitology / Q000821:virology*; D010935:Plant Diseases / Q000276:immunology* / Q000469:parasitology / Q000821:virology*; D018517:Plant Roots / Q000821:virology; D018547:Plant Stems / Q000821:virology; D030821:Plants, Genetically Modified; D019176:Potyviridae / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014454:Uganda
chemical_list: D034741:RNA, Small Interfering