title: Angiotensin II-induced mitochondrial reactive oxygen species and peroxiredoxin-3 expression in cardiac fibroblasts.
abstract: OBJECTIVE
  The aim of this study was to determine whether angiotensin II (ANG II) affects the protein and mRNA expression of the mitochondrial antioxidant peroxiredoxin-3 (Prx-3) in cardiac fibroblasts, thereby contributing to the oxidative stress in the myocardium.
  
  
  METHODS
  Cardiac fibroblasts (passage 2) from normal male adult rats were cultured to confluency and incubated in Dulbecco's modified Eagle's medium for 24  h. The cells were then preincubated with(out) the tested inhibitors for 1  h and further incubated with/without ANG II (1 μmol/l) for 24  h.
  
  
  RESULTS
  ANG II increased (P < 0.001) the mitochondrial production of reactive oxygen species in cardiac fibroblasts from 187.8 ± 38.6 to 313.8 ± 30.6 a.u./mg mitochondrial protein (n = 15). ANG II decreased (P < 0.01) the mRNA and protein expression of Prx-3 by 36.9 ± 3.0% and 29.7 ± 2.7% (n = 4), respectively. The ANG II-induced decrease in mRNA expression of Prx-3 was prevented by the angiotensin type 1 receptor blocker, losartan but not by the angiotensin type 2 receptor blocker, PD 123 319.
  
  
  CONCLUSIONS
  Our data indicate that ANG II-stimulated mitochondrial reactive oxygen species production in rat cardiac fibroblasts is accompanied by a reduction in the expression of the mitochondrial antioxidant Prx-3, and thereby potentially contributing to oxidative stress in the myocard.
authors: Hypertension and Cardiovascular Rehabilitation Unit, Department of Cardiovascular Sciences, Catholic University of Leuven (KULeuven), Leuven, Belgium. paul.lijnen@med.kuleuven.be
authors: Paul J
authors: PJ
authors: Lijnen
authors: Yvette
authors: Y
authors: Piccart
authors: Tamara
authors: T
authors: Coenen
authors: John S
authors: JS
authors: Prihadi
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005347:Fibroblasts / Q000378:metabolism; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D061105:Peroxiredoxin III / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D000804:Angiotensin II; D061105:Peroxiredoxin III