title: Visualization of mobility by atomic force microscopy.
abstract: Intrinsically disordered regions (IDRs) of proteins are very thin and hence hard to be visualized by electron microscopy. Thus far, only high-speed atomic force microscopy (HS-AFM) can visualize them. The molecular movies identify the alignment of IDRs and ordered regions in an intrinsically disordered protein (IDP) and show undulation motion of the IDRs. The visualized tail-like structures contain the information of mechanical properties of the IDRs. Here, we describe methods of HS-AFM visualization of IDPs and methods of analyzing the obtained images to characterize IDRs.
authors: Department of Physics and Bio-AFM Frontier Research Center, Kanazawa University, Kanazawa, Japan. tando@staff.kanazawa-u.ac.jp
authors: Toshio
authors: T
authors: Ando
authors: Noriyuki
authors: N
authors: Kodera
mesh_terms: D001696:Biomechanical Phenomena; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism*; D018625:Microscopy, Atomic Force / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009068:Movement
chemical_list: D055767:Immobilized Proteins