title: Production of biodiesel from plant oil hydrolysates using an Aspergillus oryzae whole-cell biocatalyst highly expressing Candida antarctica lipase B.
abstract: For enzymatic biodiesel production from plant oil hydrolysates, an Aspergillus oryzae whole-cell biocatalyst that expresses Candida antarctica lipase B (r-CALB) with high esterification activity was developed. Each of soybean and palm oils was hydrolyzed using Candida rugosa lipase, and the resultant hydrolysates were subjected to esterification where immobilized r-CALB was used as a catalyst. In esterification, r-CALB afforded a methyl ester content of more than 90% after 6 h with the addition of 1.5 M equivalents of methanol. Favorably, stepwise additions of methanol and a little water were unnecessary for maintaining the lipase stability of r-CALB during esterification. During long-term esterification in a rotator, r-CALB can be recycled for 20 cycles without a significant loss of lipase activity, resulting in a methyl ester content of more than 90% even after the 20th batch. Therefore, the presented reaction system using r-CALB shows promise for biodiesel production from plant oil hydrolysates.
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Kobe 657-8501, Japan.
authors: Daisuke
authors: D
authors: Adachi
authors: Shinji
authors: S
authors: Hama
authors: Kazunori
authors: K
authors: Nakashima
authors: Takayuki
authors: T
authors: Bogaki
authors: Chiaki
authors: C
authors: Ogino
authors: Akihiko
authors: A
authors: Kondo
mesh_terms: D001236:Aspergillus oryzae / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D055162:Biocatalysis / Q000187:drug effects; D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D018914:Cells, Immobilized / Q000378:metabolism; D004795:Enzyme Stability / Q000187:drug effects; D004951:Esterification / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D006868:Hydrolysis / Q000187:drug effects; D008049:Lipase / Q000378:metabolism*; D000432:Methanol / Q000494:pharmacology; D000073878:Palm Oil; D010938:Plant Oils / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013997:Time Factors; D014867:Water / Q000494:pharmacology
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D005656:Fungal Proteins; D010938:Plant Oils; D011994:Recombinant Proteins; D014867:Water; D000073878:Palm Oil; D008049:Lipase; C431349:lipase B, Candida antarctica; D000432:Methanol