title: Phylogenetically diverse TT virus viremia among pregnant women.
abstract: Infections during pregnancy have been suggested to be involved in childhood leukemias. We used high-throughput sequencing to describe the viruses most readily detectable in serum samples of pregnant women. Serum DNA of 112 mothers to leukemic children was amplified using whole genome amplification. Sequencing identified one TT virus (TTV) isolate belonging to a known type and two putatively new TTVs. For 22 mothers, we also performed TTV amplification by general primer PCR before sequencing. This detected 39 TTVs, two of which were identical to the TTVs found after whole genome amplification. Altogether, we found 40 TTV isolates, 29 of which were putatively new types (similarities ranging from 89% to 69%). In conclusion, high throughput sequencing is useful to describe the known or unknown viruses that are present in serum samples of pregnant women.
authors: Depts. of Laboratory Medicine, Medical Epidemiology & Biostatistics, Karolinska Institutet and Karolinska University Hospital, 17177 Stockholm, Sweden.
authors: Davit
authors: D
authors: Bzhalava
authors: Johanna
authors: J
authors: Ekström
authors: Fredrik
authors: F
authors: Lysholm
authors: Emilie
authors: E
authors: Hultin
authors: Helena
authors: H
authors: Faust
authors: Bengt
authors: B
authors: Persson
authors: Matti
authors: M
authors: Lehtinen
authors: Ethel-Michele
authors: EM
authors: de Villiers
authors: Joakim
authors: J
authors: Dillner
mesh_terms: D000595:Amino Acid Sequence; D002648:Child; D002675:Child, Preschool; D004266:DNA Virus Infections / Q000453:epidemiology / Q000635:transmission / Q000821:virology; D004279:DNA, Viral / Q000097:blood; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D018445:Infectious Disease Transmission, Vertical; D007938:Leukemia / Q000453:epidemiology / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000453:epidemiology / Q000821:virology*; D017422:Sequence Analysis, DNA; D022783:Torque teno virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014766:Viremia / Q000453:epidemiology / Q000635:transmission / Q000821:virology*
chemical_list: D004279:DNA, Viral