title: A target-activated autocatalytic DNAzyme amplification strategy for the assay of base excision repair enzyme activity.
abstract: Based on a target-activated autocatalytic DNAzyme amplification strategy, novel fluorescence sensing platforms were constructed for highly sensitive and selective assay of base excision repair enzyme activity. By using a rolling circle amplification (RCA)-coupled amplification cascade, an extremely low detection limit (0.002 U mL(-1)) was achieved.
authors: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors: Liangliang
authors: L
authors: Zhang
authors: Jingjin
authors: J
authors: Zhao
authors: Jianhui
authors: J
authors: Jiang
authors: Ruqin
authors: R
authors: Yu
mesh_terms: D055162:Biocatalysis; D001681:Biological Assay; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004260:DNA Repair / Q000235:genetics*; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D005453:Fluorescence; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques; D051981:Uracil-DNA Glycosidase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D021881:DNA, Catalytic; D004247:DNA; D051981:Uracil-DNA Glycosidase