title: Formation of spherulitic amyloid β aggregate by anionic liposomes.
abstract: Alzheimer's disease is the most common form of senile dementia. This neurodegenerative disorder is characterized by an amyloid deposition in senile plaques, composed primarily of fibrils of an aggregated peptide, amyloid β (Aβ). The modeling of a senile plaque formation on a model neuronal membrane under the physiological condition is an attractive issue. In this study, we used anionic liposomes to model the senile plaque formation by Aβ. The growth behavior of amyloid Aβ fibrils was directly observed, revealing that the induction of the spherulitic Aβ aggregates could result from the growth of seeds in the presence of anionic liposomes. The seeds of Aβ fibrils strongly interacted with negatively charged liposome and the subsequent association of the seeds were induced to form the seed cluster with many growth ends, which is advantageous for the formation of spherulitic Aβ aggregates. Therefore, anionic liposomes mediated not only fibril growth but also the aggregation process. These results imply that anionic liposome membranes would affect the aggregate form of Aβ fibrils. The modeling of senile plaque reported here is considered to have great potential for study on the amyloidosis.
authors: Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
authors: Toshinori
authors: T
authors: Shimanouchi
authors: Naoya
authors: N
authors: Shimauchi
authors: Ryo
authors: R
authors: Ohnishi
authors: Nachi
authors: N
authors: Kitaura
authors: Hisashi
authors: H
authors: Yagi
authors: Yuji
authors: Y
authors: Goto
authors: Hiroshi
authors: H
authors: Umakoshi
authors: Ryoichi
authors: R
authors: Kuboi
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000686:Amyloidosis / Q000378:metabolism; D000838:Anions / Q000737:chemistry; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008956:Models, Chemical; D010446:Peptide Fragments / Q000737:chemistry*; D058225:Plaque, Amyloid / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D000838:Anions; D008081:Liposomes; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40)