title: Coagulases as determinants of protective immune responses against Staphylococcus aureus.
abstract: During infection, Staphylococcus aureus secretes two coagulases (Coa and von Willebrand factor binding protein [vWbp]), which, following an association with host prothrombin and fibrinogen, form fibrin clots and enable the establishment of staphylococcal disease. Within the genomes of different S. aureus isolates, coagulase gene sequences are variable, and this has been exploited for a classification of types. We show here that antibodies directed against the variable prothrombin binding portion of coagulases confer type-specific immunity through the neutralization of S. aureus clotting activity and protection from staphylococcal disease in mice. By combining variable portions of coagulases from North American isolates into hybrid Coa and vWbp proteins, a subunit vaccine that provided protection against challenge with different coagulase-type S. aureus strains in mice was derived.
authors: Department of Microbiology, University of Chicago, Chicago, Illinois, USA.
authors: Molly
authors: M
authors: McAdow
authors: Andrea C
authors: AC
authors: DeDent
authors: Carla
authors: C
authors: Emolo
authors: Alice G
authors: AG
authors: Cheng
authors: Barry N
authors: BN
authors: Kreiswirth
authors: Dominique M
authors: DM
authors: Missiakas
authors: Olaf
authors: O
authors: Schneewind
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D001665:Binding Sites; D003030:Coagulase / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000276:immunology*; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology*; D016680:Genome, Bacterial; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding; D011817:Rabbits; D013203:Staphylococcal Infections / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D013209:Staphylococcal Vaccines / Q000276:immunology; D013211:Staphylococcus aureus / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D013997:Time Factors
chemical_list: D000907:Antibodies, Bacterial; D003030:Coagulase; D013209:Staphylococcal Vaccines