title: Dopaminergic imaging of nonmotor manifestations in a rat model of Parkinson's disease by fMRI.
abstract: Nonmotor manifestations determine the life quality of patients with Parkinson's disease (PD). Identification of the nonmotor symptoms in PD as definite changes will represent a milestone in its diagnosis. Outcome measures that characterize nonmotor manifestations with specificity for dopaminergic deficiency are essential to that goal. Pain is a prevalent sensory disturbance in PD patients. The prevalence was reported to be up to 83%. Nociceptive stimuli under normal conditions elicit decreases in cerebral blood volume (CBV) in the striatum via dopaminergic neurotransmission. This nociception-induced CBV response is potentially to be defined as a characteristic of the pain symptom of PD. To validate this concept, steady-state CBV-weighted functional magnetic resonance imaging with iron oxide nanoparticles was employed to measure CBV changes in parkinsonian rats. Tyrosine hydroxylase immunohistology was used to identify the dopaminergic integrity to corroborate the imaging findings. Additional experiments that tested pain responses in parkinsonism were also carried out. The results revealed that the lesioned striatum exhibited a weakened CBV decrease in response to the nociceptive stimulus. This weakened CBV response occurred mainly in areas with dopaminergic denervation. A strong correspondence was observed between the distributions of the nociception-induced CBV responses and dopaminergic innervation. The persisting CBV signals in the striatum were abolished by the D2/D3 antagonist eticlopride. The findings of these behavioral, neuroimaging, immunohistological, and pharmacological experiments demonstrate that pain in a rat model of PD can be characterized by nociception induced striatal CBV signal changes with specificity for dopaminergic dysfunction.
authors: Institute of Biomedical Sciences, Academic Sinica, Taipei, Taiwan; Functional and Micro-magnetic Resonance Imaging Center, Academic Sinica, Taipei, Taiwan.
authors: Chiao-Chi V
authors: CC
authors: Chen
authors: Department of Neurology, University of North Carolina at Chapel Hill, NC, USA; Biomedical Research Imaging Center, University of North Carolina at Chapel Hill, NC, USA; Department of Biomedical Engineering, University of North Carolina at Chapel Hill, NC, USA. Electronic address: shihy@unc.edu.
authors: Yen-Yu I
authors: YY
authors: Shih
authors: Institute of Biomedical Sciences, Academic Sinica, Taipei, Taiwan; Functional and Micro-magnetic Resonance Imaging Center, Academic Sinica, Taipei, Taiwan. Electronic address: bmcchen@ibms.sinica.edu.tw.
authors: Chen
authors: C
authors: Chang
mesh_terms: D000818:Animals; D001810:Blood Volume / Q000187:drug effects / Q000502:physiology; D001811:Blood Volume Determination / Q000379:methods; D001921:Brain / Q000187:drug effects / Q000503:physiopathology*; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000502:physiology; D004298:Dopamine / Q000378:metabolism*; D018492:Dopamine Antagonists / Q000494:pharmacology; D005290:Ferric Compounds; D007839:Functional Laterality; D007150:Immunohistochemistry; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D053768:Metal Nanoparticles; D059226:Nociceptive Pain / Q000503:physiopathology*; D016627:Oxidopamine; D020734:Parkinsonian Disorders / Q000503:physiopathology*; D017208:Rats, Wistar; D012457:Salicylamides / Q000494:pharmacology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D018492:Dopamine Antagonists; D005290:Ferric Compounds; D012457:Salicylamides; C000499:ferric oxide; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase; C045989:eticlopride; D004298:Dopamine