title: Microsatellite markers for the endangered root holoparasite Dactylanthus taylorii (Balanophoraceae) from 454 pyrosequencing.
abstract: OBJECTIVE
  Microsatellite loci were isolated and developed as polymorphic markers for the New Zealand endemic root holoparasite Dactylanthus taylorii for use in population and conservation genetics studies.
  
  
  RESULTS
  Shotgun 454 pyrosequencing was performed on genomic DNA pooled from three individuals of D. taylorii. From 61709 individual sequence reads, primers for 753 microsatellite loci were developed in silico and 72 of these were tested for consistent amplification and variability. Ten microsatellite loci were found to be polymorphic and consistently scorable when screened in 44 individuals from five geographically distant populations. The number of alleles per locus ranged from four to 16 with an average of 9.7, and average observed heterozygosity per locus was between 0.182 and 0.634.
  
  
  CONCLUSIONS
  These polymorphic microsatellite markers establish an important resource for ongoing conservation initiatives and planned population genetic studies of D. taylorii.
authors: Institute of Molecular BioSciences, Massey University, Palmerston North, New Zealand.
authors: Todd G B
authors: TG
authors: McLay
authors: Jennifer A
authors: JA
authors: Tate
authors: V Vaughan
authors: VV
authors: Symonds
mesh_terms: D000483:Alleles; D031111:Balanophoraceae / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D056426:Genetic Loci; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing; D056626:Inflorescence / Q000145:classification / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers