title: The movement protein encoded by gene 3 of rice transitory yellowing virus is associated with virus particles.
abstract: Gene 3 in the genomes of several plant-infecting rhabdoviruses, including rice transitory yellowing virus (RTYV), has been postulated to encode a cell-to-cell movement protein (MP). Trans-complementation experiments using a movement-defective tomato mosaic virus and the P3 protein of RTYV, encoded by gene 3, facilitated intercellular transport of the mutant virus. In transient-expression experiments with the GFP-fused P3 protein in epidermal leaf cells of Nicotiana benthamiana, the P3 protein was associated with the nucleus and plasmodesmata. Immunogold-labelling studies of thin sections of RTYV-infected rice plants using an antiserum against Escherichia coli-expressed His(6)-tagged P3 protein indicated that the P3 protein was located in cell walls and on virus particles. In Western blots using antisera against E. coli-expressed P3 protein and purified RTYV, the P3 protein was detected in purified RTYV, whilst antiserum against purified RTYV reacted with the E. coli-expressed P3 protein. After immunogold labelling of crude sap from RTYV-infected rice leaves, the P3 protein, as well as the N protein, was detected on the ribonucleocapsid core that emerged from partially disrupted virus particles. These results provide evidence that the P3 protein of RTYV, which functions as a viral MP, is a viral structural protein and seems to be associated with the ribonucleocapsid core of virus particles.
authors: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Akihiro
authors: A
authors: Hiraguri
authors: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Hiroyuki
authors: H
authors: Hibino
authors: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Takaharu
authors: T
authors: Hayashi
authors: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Osamu
authors: O
authors: Netsu
authors: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Takumi
authors: T
authors: Shimizu
authors: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Tamaki
authors: T
authors: Uehara-Ichiki
authors: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Toshihiro
authors: T
authors: Omura
authors: Gene Research Center, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509 Japan.
authors: Nobumitsu
authors: N
authors: Sasaki
authors: Gene Research Center, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509 Japan.
authors: Hiroshi
authors: H
authors: Nyunoya
authors: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-8666, Japan.
authors: Takahide
authors: T
authors: Sasaya
mesh_terms: D002473:Cell Wall / Q000378:metabolism / Q000821:virology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000378:metabolism / Q000821:virology; D053830:Plant Viral Movement Proteins / Q000235:genetics* / Q000378:metabolism; D031425:Plasmodesmata / Q000378:metabolism / Q000821:virology; D012209:Rhabdoviridae / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism / Q000821:virology; D017898:Tobamovirus / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000235:genetics* / Q000378:metabolism
chemical_list: D053830:Plant Viral Movement Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins