title: Effects of N-n-butyl haloperidol iodide on the rat myocardial sarcoplasmic reticulum Ca(2+)-ATPase during ischemia/reperfusion.
abstract: We have previously shown that N-n-butyl haloperidol iodide (F(2)), a newly synthesized compound, reduces ischemia/reperfusion (I/R) injury by preventing intracellular Ca(2+) overload through inhibiting L-type calcium channels and outward current of Na(+)/Ca(2+) exchanger. This study was to investigate the effects of F(2) on activity and protein expression of the rat myocardial sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) during I/R to discover other molecular mechanisms by which F(2) maintains intracellular Ca(2+) homeostasis. In an in vivo rat model of myocardial I/R achieved by occluding coronary artery for 30-60 min followed by 0-120 min reperfusion, treatment with F(2) (0.25, 0.5, 1, 2 and 4 mg/kg, respectively) dose-dependently inhibited the I/R-induced decrease in SERCA activity. However, neither different durations of I/R nor different doses of F(2) altered the expression levels of myocardial SERCA2a protein. These results indicate that F(2) exerts cardioprotective effects against I/R injury by inhibiting I/R-mediated decrease in SERCA activity by a mechanism independent of SERCA2a protein levels modulation.
authors: Department of Pharmacology, Shantou University Medical College, Shantou 515041, Guangdong, China.
authors: Yan-Mei
authors: YM
authors: Zhang
authors: Chun-Yan
authors: CY
authors: Wang
authors: Fu-Chun
authors: FC
authors: Zheng
authors: Fen-Fei
authors: FF
authors: Gao
authors: Yi-Cun
authors: YC
authors: Chen
authors: Zhan-Qin
authors: ZQ
authors: Huang
authors: Zheng-Yuan
authors: ZY
authors: Xia
authors: Michael G
authors: MG
authors: Irwin
authors: Wei-Qiu
authors: WQ
authors: Li
authors: Xing-Ping
authors: XP
authors: Liu
authors: Yan-Shan
authors: YS
authors: Zheng
authors: Han
authors: H
authors: Xu
authors: Gang-Gang
authors: GG
authors: Shi
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D006220:Haloperidol / Q000031:analogs & derivatives* / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D046529:Microscopy, Electron, Transmission; D015428:Myocardial Reperfusion Injury / Q000201:enzymology* / Q000473:pathology; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012519:Sarcoplasmic Reticulum / Q000187:drug effects* / Q000201:enzymology; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
chemical_list: D002316:Cardiotonic Agents; C482574:N-n-butyl haloperidol iodide; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D006220:Haloperidol