title: Gene therapy of gastric cancer using LIGHT-secreting human umbilical cord blood-derived mesenchymal stem cells.
abstract: BACKGROUND
  Mesenchymal stem cells (MSCs) have the ability to migrate into tumors and therefore are potential vehicles for the therapy of malignant diseases. In this study, we investigated the use of umbilical cord blood mesenchymal stem cells (UCB-MSCs) as carriers for a constant source of transgenic LIGHT (TNFSF14) to target tumor cells in vivo.
  
  
  METHODS
  Lentiviral vectors carrying LIGHT genes were constructed, producing viral particles with a titer of 2 × 10(8) TU/L. Fourteen days after UCB-MSCs transfected by LIGHT gene packaged lentivirus had been injected into mouse gastric cancer models, the expression levels of LIGHT mRNA and protein were detected by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Then the tumors' approximate volumes were measured.
  
  
  RESULTS
  The treatment with MSC-LIGHT demonstrated a strong suppressive effect on tumor growth compared to treatment with MSC and NaCl (p < 0.001). Examination of pathological sections of the tumor tissues showed that the areas of tumor necrocis in the MSC-LIGHT group were larger than those in the MSC group. Moreover, we found that MSCs with LIGHT were able to significantly induce apoptosis of tumor cells. The expression levels of LIGHT mRNA and protein were significantly higher in the UCB-MSCs with the LIGHT gene than the levels in UCB-MSCs (p < 0.001).
  
  
  CONCLUSIONS
  These results suggest that UCB-MSCs carrying the LIGHT gene have the potential to be used as effective delivery vehicles in the treatment of gastric cancers.
authors: Department of Central Laboratory, Qingdao Municipal Hospital, Qingdao, China.
authors: Xinhong
authors: X
authors: Zhu
authors: Dongming
authors: D
authors: Su
authors: Shiying
authors: S
authors: Xuan
authors: Guiliang
authors: G
authors: Ma
authors: Zhenbo
authors: Z
authors: Dai
authors: Tongyun
authors: T
authors: Liu
authors: Dongqi
authors: D
authors: Tang
authors: Weizheng
authors: W
authors: Mao
authors: Chenfang
authors: C
authors: Dong
mesh_terms: D000818:Animals; D001483:Base Sequence; D004195:Disease Models, Animal; D005312:Fetal Blood / Q000166:cytology*; D005786:Gene Expression Regulation; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000821:virology; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D013274:Stomach Neoplasms / Q000473:pathology / Q000628:therapy*; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
chemical_list: C505914:TNFSF14 protein, human; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14