title: Growth and differentiation characteristics of equine mesenchymal stromal cells derived from different sources.
abstract: Multipotent mesenchymal stromal cells (MSCs) are a promising therapeutic tool for the treatment of equine tendon and other musculoskeletal injuries. While bone marrow is considered the 'gold standard' source of these cells, various other tissues contain MSCs with potentially useful features. The aim of this study was to compare clinically relevant characteristics of MSCs derived from bone marrow, umbilical cord blood and tissue and from adipose tissue and tendon. Cell yield, proliferation, migration, tendon marker expression and differentiation into adipocytes, chondrocytes and osteoblasts was assessed, quantified and compared. MSC numbers obtained from adipose, tendon or umbilical cord tissues were 222-fold higher than those obtained from bone marrow or cord blood. Cells derived from tendon and adipose tissues exhibited most rapid proliferation. Osteogenic differentiation was most prominent in MSCs derived from bone marrow, and was weak in MSCs derived from umbilical cord blood and tissue. In contrast, the highest levels of chondrogenic differentiation were observed in MSCs derived from these sources. Collagen 1A2 expression was highest in adipose- and tendon-derived MSCs, while scleraxis expression was highest in cord blood- and in tendon-derived MSCs. The findings indicate that MSCs from different sources display significantly diverse properties that may impact on their therapeutic application.
authors: Large Animal Clinic for Surgery, Universität Leipzig, An den Tierkliniken 21, 04103 Leipzig, Germany. burk@rz.uni-leipzig.de
authors: Janina
authors: J
authors: Burk
authors: Iris
authors: I
authors: Ribitsch
authors: Claudia
authors: C
authors: Gittel
authors: Henriette
authors: H
authors: Juelke
authors: Cornelia
authors: C
authors: Kasper
authors: Carsten
authors: C
authors: Staszyk
authors: Walter
authors: W
authors: Brehm
mesh_terms: D000818:Animals; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D005312:Fetal Blood / Q000166:cytology; D005786:Gene Expression Regulation / Q000502:physiology; D006736:Horses / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D013710:Tendons / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D015415:Biomarkers