title: Influence of "glow discharge plasma" as an external stimulus on the self-assembly, morphology and binding affinity of gold nanoparticle-streptavidin conjugates.
abstract: In this study, we investigate the influence of glow discharge plasma (GDP) on the self-assembly, morphology and binding affinity of streptavidin coated gold nanoparticles (Au-NP-SV) and biotinylated antibody (bAb) adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate. Atomic force microscope (AFM) was used to image the pre- and post-GDP treated samples. The analysis of the AFM images showed a considerable change in the aggregation and morphology of Au-NP-conjugates after treatment with GDP. To our knowledge, this is the first report on using GDP to enhance and speed-up the aggregation (sintering) of adsorbed NP biomolecular conjugates. These results show a promising route that could be generalized for other NPs and their conjugates. It can also be considered as an alternative and cheap aggregation method for controlling the binding affinity of biomolecular species on different surfaces with interesting applications.
authors: Department of Chemistry, School of Sciences and Engineering, The American University in Cairo (AUC), AUC Avenue, P.O. Box 74, New Cairo 11835, Egypt.
authors: Wael
authors: W
authors: Mamdouh
authors: Advanced Nanotechnology Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Yingzhi
authors: Y
authors: Li
authors: Department of Chemistry, School of Sciences and Engineering, The American University in Cairo (AUC), AUC Avenue, P.O. Box 74, New Cairo 11835, Egypt.
authors: Sherif M
authors: SM
authors: Shawky
authors: Department of Chemistry, School of Sciences and Engineering, The American University in Cairo (AUC), AUC Avenue, P.O. Box 74, New Cairo 11835, Egypt.|Yousef Jameel Science & Technology Research Center, the American University in Cairo, AUC Avenue, P.O. Box 74, New Cairo 11835, Egypt.
authors: Hassan M E
authors: 0000-0003-2047-4222
authors: HME
authors: Azzazy
authors: Advanced Nanotechnology Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Chang-Jun
authors: CJ
authors: Liu
mesh_terms: D000327:Adsorption; D000906:Antibodies / Q000737:chemistry; D001681:Biological Assay; D019921:Biotinylation; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D058726:Nanoconjugates / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000906:Antibodies; D058726:Nanoconjugates; D006046:Gold; D006108:Graphite; D019809:Streptavidin