title: Mycobacterium tuberculosis complex enhances susceptibility of CD4 T cells to HIV through a TLR2-mediated pathway.
abstract: Among HIV-infected individuals, co-infection with Mycobacterium tuberculosis is associated with faster progression to AIDS. We investigated the hypothesis that M. bovis BCG and M. tuberculosis (Mtb complex) could enhance susceptibility of CD4+ cells to HIV infection. Peripheral blood mononuclear cells (PBMCs) collected from healthy donors were stimulated with M. bovis BCG, M. tuberculosis CDC1551 and M. smegmatis MC(2)155, and stimulated CD4+ cells were infected with R5-and X4-tropic single replication-competent pseudovirus. CD4+ cells stimulated with Mtb complex showed enhanced infection with R5- and X4-tropic HIV, compared to unstimulated cells or cells stimulated with M. smegmatis (p<0.01). Treatment with TLR2 siRNA reversed the increased susceptibility of CD4+ cells with R5- and X4-tropic virus induced by Mtb complex. These findings suggest that TB infection and/or BCG vaccination may be a risk factor for HIV acquisition.
authors: Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
authors: Seema M
authors: SM
authors: Thayil
authors: Ya-Chi
authors: YC
authors: Ho
authors: Robert C
authors: RC
authors: Bollinger
authors: Joel N
authors: JN
authors: Blankson
authors: Robert F
authors: RF
authors: Siliciano
authors: Petros C
authors: PC
authors: Karakousis
authors: Kathleen R
authors: KR
authors: Page
mesh_terms: D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology* / Q000821:virology*; D004198:Disease Susceptibility / Q000276:immunology / Q000382:microbiology; D005786:Gene Expression Regulation / Q000276:immunology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D009163:Mycobacterium bovis / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D055815:Young Adult
chemical_list: C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2