title: [Callus induction of Liriope muscari and its dynamic accumulation of polysaccharide].
abstract: OBJECTIVE
  To study the induction rate of callus tissue in four different explants of stem, root, leaf and fruit of Liriope muscari.
  
  
  METHODS
  The effect of 2,4-D, sugar and illumination on callus succeeding preservation was analyzed. The dynamic accumlation of polysaccharide in callus was described. The polysaccharide content among callus, tissue culture seedings and field seedings was compared.
  
  
  CONCLUSIONS
  The callus induction rate of stem was the highest. The optimal concentration range of 2,4-D was 1.5-2.0 mg/L, then the induction rate was 87.5%. When the 2,4-D conncentration was 0.5-1.0 mg/L, and the sucrose concentration was 20 g/L, the multiplication coefficient was highest. The illumination condition did not influence the effect of callus succeeding preservation. The content of callus polysaccharide continuously increased for 60 d. The growth rate of callus was reached the highest level from 40 d to 60 d. Polysaccharide content in root of tissue culture seeding was higher than that of the field seeding.
authors: Agricultural Bio-resources Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350003, China. wxf125@163.com
authors: Xue-feng
authors: XF
authors: Wan
authors: Ying-zhen
authors: YZ
authors: Huang
authors: Zhong-xiong
authors: ZX
authors: Lai
authors: Jing-ying
authors: JY
authors: Chen
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D008027:Light; D031424:Liriope Plant / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000379:methods
chemical_list: D003470:Culture Media; D010937:Plant Growth Regulators; D011134:Polysaccharides; D015084:2,4-Dichlorophenoxyacetic Acid; D013395:Sucrose