title: Microsatellites for Tetracentron sinense (Trochodendraceae), a Tertiary relict endemic to East Asia.
abstract: OBJECTIVE
  Tetracentron sinense (Trochodendraceae) is a Tertiary relict endemic to East Asia. Microsatellite markers were developed and characterized to investigate the population genetics of the species.
  
  
  RESULTS
  Microsatellite markers were isolated from the genome of T. sinense using the protocol of Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO). Eight polymorphic microsatellite markers were assessed in 44 samples collected from three wild populations. The number of alleles observed for each locus ranged from two to five. The observed and expected heterozygosities ranged from 0.0000 to 0.9375 and 0.0000 to 0.7681, respectively.
  
  
  CONCLUSIONS
  The microsatellite markers will be helpful in further studies of the population genetics and phylogeography of T. sinense.
authors: Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, People's Republic of China.
authors: Zhengyan
authors: Z
authors: Yang
authors: Ranran
authors: R
authors: Lu
authors: Chengcheng
authors: C
authors: Tao
authors: Shaotian
authors: S
authors: Chen
authors: Yunheng
authors: Y
authors: Ji
mesh_terms: D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D056426:Genetic Loci; D005819:Genetic Markers; D005828:Genetics, Population; D019684:Magnoliopsida / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000145:classification / Q000235:genetics; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers