title: Prevalence and genetic diversity of Bartonella species in sika deer (Cervus nippon) in Japan.
abstract: We report the first description of Bartonella prevalence and genetic diversity in 64 Honshu sika deer (Cervus nippon centralis) and 18 Yezo sika deer (Cervus nippon yesoensis) in Japan. Overall, Bartonella bacteremia prevalence was 41.5% (34/82). The prevalence in wild deer parasitized with ticks and deer keds was 61.8% (34/55), whereas no isolates were detected in captive deer (0/27) free of ectoparasites. The isolates belonged to 11 genogroups based on a combination of the gltA and rpoB gene sequences. Phylogenetic analysis of concatenated sequences of the ftsZ, gltA, ribC, and rpoB genes of 11 representative isolates showed that Japanese sika deer harbor three Bartonella species, including B. capreoli and two novel Bartonella species. All Yezo deer's isolates were identical to B. capreoli B28980 strain isolated from an elk in the USA, based on the sequences of the ftsZ, gltA, and rpoB genes. In contrast, the isolates from Honshu deer showed a higher genetic diversity.
authors: Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors: Shingo
authors: S
authors: Sato
authors: Hidenori
authors: H
authors: Kabeya
authors: Mari
authors: M
authors: Yamazaki
authors: Shinako
authors: S
authors: Takeno
authors: Kazuo
authors: K
authors: Suzuki
authors: Shinichi
authors: S
authors: Kobayashi
authors: Kousaku
authors: K
authors: Souma
authors: Takayoshi
authors: T
authors: Masuko
authors: Bruno B
authors: BB
authors: Chomel
authors: Soichi
authors: S
authors: Maruyama
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary*; D001473:Bartonella / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001474:Bartonella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000235:genetics*; D003670:Deer / Q000382:microbiology*; D014644:Genetic Variation; D007564:Japan / Q000453:epidemiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D015995:Prevalence
chemical_list: D004269:DNA, Bacterial