title: Identification and elimination of heterophilic antibody interference during antibody pair screening.
abstract: High background interference during the antibody pair screening process is inevitable. In this study, we found that the high background was associated with heterophilic antibody interference introduced by the application of ascites-derived monoclonal antibodies when conducting large-scale antibody pair screening against different proteins. To eliminate antibody-associated heterophilic antibody interference, both blocking with mouse normal sera and antigen-mediated affinity chromatography were used, resulting in significant improvement in pairing performance and in antibody pair screening efficiency.
authors: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China. youyonglu@hsc.pku.edu.cn
authors: Yuan
authors: Y
authors: Wang
authors: Hanfu
authors: H
authors: Wei
authors: Qin
authors: Q
authors: Pan
authors: Zhaohui
authors: Z
authors: Wang
authors: Rui
authors: R
authors: Xing
authors: Wenmei
authors: W
authors: Li
authors: Jun
authors: J
authors: Zhang
authors: Meng
authors: M
authors: Ding
authors: Jiao
authors: J
authors: Guo
authors: Lin
authors: L
authors: Wu
authors: Youyong
authors: Y
authors: Lu
authors: Siqi
authors: S
authors: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology*; D016477:Artifacts; D002417:Cattle; D007118:Immunoassay / Q000379:methods*; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins