title: Asymmetric synthesis of novel N-(1-phenyl-2,3-dihydroxypropyl)arachidonylamides and evaluation of their anti-inflammatory activity.
abstract: OBJECTIVE
  To design and synthesize novel N-(1-phenyl-2,3-dihydroxypropyl)arachidonylamides and evaluate their analgesic and anti-inflammatory potential.
  
  
  METHODS
  The murine macrophage cell line RAW 264.7 has been widely used as a model for inflammatory responses in vitro. Our model consists of cultured monolayers of RAW 264.7 cells in which media concentrations of 15-deoxy-Δ(13,14)-PGJ2 (PGJ) are measured by ELISA following LPS (10ng/ml) stimulation and treatment with 0.1, 0.3, 1.0, 3.0 and 10μM concentrations of the compounds.
  
  
  RESULTS
  Our data indicate that several of our compounds have the capacity to increase production of PGJ and may also increase the occurrence of programmed cell death (apoptosis).
  
  
  CONCLUSIONS
  Thus these agents are potential candidates for the therapy of conditions characterized by ongoing (chronic) inflammation and its associated pain.
authors: Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.
authors: Padmanabha V
authors: PV
authors: Kattamuri
authors: Rebecca
authors: R
authors: Salmonsen
authors: Catherine
authors: C
authors: McQuain
authors: Sumner
authors: S
authors: Burstein
authors: Hao
authors: H
authors: Sun
authors: Guigen
authors: G
authors: Li
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000138:chemical synthesis* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001095:Arachidonic Acids / Q000138:chemical synthesis* / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D007118:Immunoassay; D007202:Indicators and Reagents; D051379:Mice; D015230:Prostaglandin D2 / Q000031:analogs & derivatives / Q000096:biosynthesis; D043884:Receptor, Cannabinoid, CB1 / Q000187:drug effects / Q000378:metabolism; D043885:Receptor, Cannabinoid, CB2 / Q000187:drug effects / Q000378:metabolism
chemical_list: C097240:15-deoxy-delta(12,14)-prostaglandin J2; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001095:Arachidonic Acids; D007202:Indicators and Reagents; D043884:Receptor, Cannabinoid, CB1; D043885:Receptor, Cannabinoid, CB2; D015230:Prostaglandin D2