title: Development and characterization of multiplex panels of microsatellite markers for Syphacia obvelata, a parasite of the house mouse (Mus musculus), using a high throughput DNA sequencing approach.
abstract: Syphacia obvelata is a common gastro-intestinal parasitic nematode of the house mouse (Mus musculus), a prime model rodent species. Investigations of the genetic structure, variability of parasite populations and other biological aspects of this host-parasite system are limited due to the lack of genetic resources for S. obvelata. To fill this gap, we developed a set of microsatellite markers for S. obvelata, using a 454 pyrosequencing approach. We designed three multiplex panels allowing genotyping of 10 polymorphic loci and scrutinized them on 42 samples from two different regions inhabited by two different house mouse subspecies (Mus musculus musculus and M. m. domesticus). The numbers of alleles ranged from 2 to 6 with mean observed heterozygosities 0.1476 and 0.2095 for domesticus and musculus worms, respectively. The described markers will facilitate further studies on population biology and co-evolution of this host-parasite system.
authors: Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Květná 8, 603 65 Brno, Czech Republic. wasim.bt@gmail.com
authors: Wasimuddin
authors: Dagmar
authors: D
authors: Čížková
authors: Alexis
authors: A
authors: Ribas
authors: Jaroslav
authors: J
authors: Piálek
authors: Joëlle Goüy
authors: JG
authors: de Bellocq
authors: Josef
authors: J
authors: Bryja
mesh_terms: D000483:Alleles; D000818:Animals; D005260:Female; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics*; D009349:Nematode Infections / Q000469:parasitology / Q000662:veterinary*; D010124:Oxyuroidea / Q000235:genetics*; D012376:Rodent Diseases / Q000469:parasitology*; D013045:Species Specificity