title: Screening of siRNA nanoparticles for delivery to airway epithelial cells using high-content analysis.
abstract: OBJECTIVE
  Delivery of siRNA to the lungs via inhalation offers a unique opportunity to develop a new treatment paradigm for a range of respiratory conditions. However, progress has been greatly hindered by safety and delivery issues. This study developed a high-throughput method for screening novel nanotechnologies for pulmonary siRNA delivery.
  
  
  METHODS
  Following physicochemical analysis, the ability of PEI-PEG-siRNA nanoparticles to facilitate siRNA delivery was determined using high-content analysis (HCA) in Calu-3 cells. Results obtained from HCA were validated using confocal microscopy. Finally, cytotoxicity of the PEI-PEG-siRNA particles was analyzed by HCA using the Cellomics multiparameter cytotoxicity assay.
  
  
  CONCLUSIONS
  PEI-PEG-siRNA nanoparticles facilitated increased siRNA uptake and luciferase knockdown in Calu-3 cells compared with PEI-siRNA.
authors: School of Pharmacy. Royal College of Surgeons in Ireland, Dublin 2, Ireland.
authors: Alan
authors: A
authors: Hibbitts
authors: Nora
authors: N
authors: Lieggi
authors: Olive
authors: O
authors: McCabe
authors: Warren
authors: W
authors: Thomas
authors: James
authors: J
authors: Barlow
authors: Fiona
authors: F
authors: O'Brien
authors: Sally-Ann
authors: SA
authors: Cryan
mesh_terms: D002452:Cell Count / Q000379:methods; D002460:Cell Line; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D004705:Endocytosis / Q000187:drug effects; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000037:antagonists & inhibitors; D006801:Humans; D007097:Imines / Q000008:administration & dosage / Q000737:chemistry; D008156:Luciferases / Q000037:antagonists & inhibitors; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry; D011095:Polyethylenes / Q000008:administration & dosage / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism
chemical_list: D004337:Drug Carriers; D007097:Imines; D011095:Polyethylenes; D034741:RNA, Small Interfering; C505405:poly(ethylene imine); D011092:Polyethylene Glycols; D008156:Luciferases; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)