title: An efficient synthesis of pyrimidine specific 2'-deoxynucleoside-5'-tetraphosphates.
abstract: An efficient chemical synthesis of pyrimidine specific 2'-deoxynucleoside-5'-tetraphosphates, such as 2'-deoxycytidine-5'-tetraphosphate (dC4P) and thymidine-5'-tetraphosphate (T4P) is described. The present three-step synthetic strategy involves monophosphorylation of 2'-deoxynucleoside using phosphorous oxychloride, conversion of 5'-monophosphate into the corresponding imidazolide salt, followed by reaction with tris[tributylammonium] triphosphate leading to the 2'-deoxynucleoside-5'-tetraphosphate in good yields.
authors: Bioorganic Chemistry Division, Life Technologies Inc., Austin, TX 78744–1832, USA. anil.kore@lifetech.com
authors: Anilkumar R
authors: AR
authors: Kore
authors: Zejun
authors: Z
authors: Xiao
authors: Annamalai
authors: A
authors: Senthilvelan
authors: Irudaya
authors: I
authors: Charles
authors: Muthian
authors: M
authors: Shanmugasundaram
authors: Sriram
authors: S
authors: Mukundarajan
authors: Balasubramanian
authors: B
authors: Srinivasan
mesh_terms: D060326:Chemistry Techniques, Synthetic / Q000191:economics / Q000379:methods*; D003853:Deoxyribonucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D007093:Imidazoles / Q000138:chemical synthesis / Q000737:chemistry; D010766:Phosphorylation; D011122:Polyphosphates / Q000138:chemical synthesis* / Q000737:chemistry; D011743:Pyrimidines / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D003853:Deoxyribonucleosides; D007093:Imidazoles; D011122:Polyphosphates; D011743:Pyrimidines