title: Phosphatase activity in barley proteins tightly bound to DNA and its development-dependent changes.
abstract: The tightly bound proteins (TBPs), a protein group that remains attached to DNA either covalently or noncovalently after deproteinization, have been found in numerous eukaryotic species. Some TBPs isolated from mammalian and yeast cells possess phosphatase or kinase activity. The aim of this study was to characterize further TBPs in barley (Hordeum vulgare) cells. The spectra of TBPs varied in different organs of barley shoots (first leaves, coleoptile, and roots) and at different developmental stages of the plant. Some barley TBPs manifested phosphatase, probably Ser/Thr or dual Ser/Thr/Tyr activity. MALDI-TOF mass spectrometry of barley TBPs identified several proteins involved in chromatin rearrangement and regulation processes, including transcription factors, serpins, protein phosphatases and protein kinases, RNA helicases, and DNA topoisomerase II.
authors: Laboratory of Molecular Oncology, Institute of Oncology, Vilnius University, P. Baublio 3b, Vilnius LT-08406, Lithuania.
authors: K
authors: K
authors: Bielskienė
authors: D
authors: D
authors: Labeikytė
authors: N
authors: N
authors: Sjakste
authors: L
authors: L
authors: Bagdonienė
authors: B
authors: B
authors: Juodka
mesh_terms: D042002:Chromatin Assembly and Disassembly / Q000502:physiology; D018548:Cotyledon / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D004250:DNA Topoisomerases, Type II / Q000032:analysis; D018744:DNA, Plant / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D001467:Hordeum / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism*; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D011494:Protein Kinases / Q000032:analysis; D020365:RNA Helicases / Q000032:analysis; D015843:Serpins / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018744:DNA, Plant; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D015843:Serpins; D011494:Protein Kinases; D010744:Phosphoric Monoester Hydrolases; D020365:RNA Helicases; D004250:DNA Topoisomerases, Type II