title: In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand.
abstract: In this article, we reported a novel approach for in situ labeling and imaging HeLa cancer cells utilizing a bifunctional aptamer (AS1411) and its fluorescent ligand, protoporphyrin IX (PPIX). In the presence of potassium ion, AS1411 folded to G-quadruplex structure, binded fluorescent ligand (PPIX) with fluorescent enhancement, and targeted the nucleolin overexpressed by cancer cells. Consequently, bioimaging of cancer cells specifically were realized by laser scanning confocal microscope. The bioimaging strategy with AS1411-PPIX complex was capable to distinguish HeLa cancer cells from normal cells unambiguously, and fluorescence imaging of cancer cells was also realized in human serum. Moreover, the bioimaging method was very facile, effective and need not any covalent modification. These results illustrated that the useful approach can provide a novel clue for bioimaging based on non-covalent bifunctional aptamer in clinic diagnosis.
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors: Jun
authors: J
authors: Ai
authors: Tao
authors: T
authors: Li
authors: Bingling
authors: B
authors: Li
authors: Yuanhong
authors: Y
authors: Xu
authors: Dan
authors: D
authors: Li
authors: Zuojia
authors: Z
authors: Liu
authors: Erkang
authors: E
authors: Wang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D054856:G-Quadruplexes; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D057054:Molecular Imaging / Q000379:methods*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011524:Protoporphyrins / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*; D013379:Substrate Specificity; D000097796:Nucleolin
chemical_list: D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D008024:Ligands; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins; D011524:Protoporphyrins; D016601:RNA-Binding Proteins; D000097796:Nucleolin; C513936:AGRO 100; C028025:protoporphyrin IX