title: Competitive binding of UBPY and ubiquitin to the STAM2 SH3 domain revealed by NMR.
abstract: To date, the signal transducing adaptor molecule 2 (STAM2) was shown to harbour two ubiquitin binding domains (UBDs) known as the VHS and UIM domains, while the SH3 domain of STAM2 was reported to interact with deubiquitinating enzymes (DUBs) like UBPY and AMSH. In the present study, NMR evidences the interaction of the STAM2 SH3 domain with ubiquitin, demonstrating that SH3 constitutes the third UBD of STAM2. Furthermore, we show that a UBPY-derived peptide can outcompete ubiquitin for SH3 binding and vice versa. These results suggest that the SH3 domain of STAM2 plays versatile roles in the context of ubiquitin mediated receptor sorting.
authors: Université de Lyon, Université Claude Bernard Lyon1, CNRS, UMR 5280 Institut des Sciences Analytiques, Villeurbanne, France.
authors: Anja
authors: A
authors: Lange
authors: Mouhamad-Baligh
authors: MB
authors: Ismail
authors: Gwladys
authors: G
authors: Rivière
authors: Maggy
authors: M
authors: Hologne
authors: Denis
authors: D
authors: Lacabanne
authors: Florence
authors: F
authors: Guillière
authors: Jean-Marc
authors: JM
authors: Lancelin
authors: Isabelle
authors: I
authors: Krimm
authors: Olivier
authors: O
authors: Walker
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001667:Binding, Competitive; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D056827:Endosomal Sorting Complexes Required for Transport / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D019906:Nuclear Magnetic Resonance, Biomolecular; D054730:Protein Interaction Domains and Motifs; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D025801:Ubiquitin / Q000737:chemistry / Q000378:metabolism*; D043222:Ubiquitin Thiolesterase / Q000737:chemistry / Q000378:metabolism*; D018909:src Homology Domains
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D056827:Endosomal Sorting Complexes Required for Transport; D011994:Recombinant Proteins; C491647:STAM2 protein, human; D025801:Ubiquitin; D010450:Endopeptidases; C541534:USP8 protein, human; D043222:Ubiquitin Thiolesterase