title: SAP30L (Sin3A-associated protein 30-like) is involved in regulation of cardiac development and hematopoiesis in zebrafish embryos.
abstract: The Sin3A-associated proteins SAP30 and SAP30L share 70% sequence identity and are part of the multiprotein Sin3A corepressor complex. They participate in gene repression events by linking members of the complex and stabilizing interactions among the protein members as well as between proteins and DNA. While most organisms have both SAP30 and SAP30L, the zebrafish is exceptional because it only has SAP30L. Here we demonstrate that SAP30L is expressed ubiquitously in embryonic and adult zebrafish tissues. Knockdown of SAP30L using morpholino-mediated technology resulted in a morphant phenotype manifesting as cardiac insufficiency and defective hemoglobinization of red blood cells. A microarray analysis of gene expression in SAP30L morphant embryos revealed changes in the expression of genes involved in regulation of transcription, TGF-beta signaling, Wnt-family transcription factors, and nuclear genes encoding mitochondrial proteins. The expression of the heart-specific nkx2.5 gene was markedly down-regulated in SAP30L morphants, and the cardiac phenotype could be partially rescued by nkx2.5 mRNA. In addition, changes were detected in the expression of genes known to be important in hemoglobin synthesis and erythropoiesis. Our results demonstrate that SAP30L regulates several transcriptional pathways in zebrafish embryos and is involved in the development of cardiac and hematopoietic systems.
authors: Paediatric Research Centre, University of Tampere School of Medicine and Tampere University Hospital, 33520 Tampere, Finland. kaisa.teittinen@uta.fi
authors: Kaisa J
authors: KJ
authors: Teittinen
authors: Toni
authors: T
authors: Grönroos
authors: Mataleena
authors: M
authors: Parikka
authors: Sini
authors: S
authors: Junttila
authors: Annemari
authors: A
authors: Uusimäki
authors: Asta
authors: A
authors: Laiho
authors: Hanna
authors: H
authors: Korkeamäki
authors: Kalle
authors: K
authors: Kurppa
authors: Hannu
authors: H
authors: Turpeinen
authors: Marko
authors: M
authors: Pesu
authors: Attila
authors: A
authors: Gyenesei
authors: Mika
authors: M
authors: Rämet
authors: Olli
authors: O
authors: Lohi
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D055785:Gene Knockdown Techniques; D006321:Heart / Q000033:anatomy & histology / Q000196:embryology*; D006410:Hematopoiesis; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D029961:Zebrafish Proteins