title: Downregulation of antigen-presenting cells in tonsil and lymph nodes of porcine reproductive and respiratory syndrome virus-infected pigs.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) can persist in different organs of infected pigs, which suggests a failure in the immune response. Antigen-presenting cells (APCs) play a pivotal role in the induction of effective T- and B-cell responses. In this study, we investigated the changes in the different APC subpopulations and T- and B-cell counts in the tonsil, retropharyngeal and mediastinal lymph nodes of pigs experimentally infected with a European PRRSV field isolate. Our results demonstrated that the expression of S100, SWC3, HLA-DR molecule and CD3 was diminished in the studied organs throughout the study, observing a significant negative correlation between viral antigen and HLA-DR expression in both retropharyngeal and mediastinal lymph nodes. In contrast, λ-light chains showed an increase during the study. Taking all into account, after PRRSV infection, no enhancement in the number of APCs and T cells was observed, suggesting an impairment of the immune function which may allow the persistence of PRRSV into the organism.
authors: Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, Córdoba University, Córdoba, Spain. v22rogoi@uco.es
authors: I M
authors: IM
authors: Rodríguez-Gómez
authors: J
authors: J
authors: Gómez-Laguna
authors: I
authors: I
authors: Barranco
authors: F J
authors: FJ
authors: Pallarés
authors: G
authors: G
authors: Ramis
authors: F J
authors: FJ
authors: Salguero
authors: L
authors: L
authors: Carrasco
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D015536:Down-Regulation; D006684:HLA-DR Antigens / Q000032:analysis; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology*; D018655:Lymphocyte Count; D008264:Macrophages / Q000276:immunology; D008482:Mediastinum; D014066:Palatine Tonsil / Q000276:immunology*; D010614:Pharynx; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D034421:Sus scrofa / Q000276:immunology; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D006684:HLA-DR Antigens