title: Expression of KITENIN and its association with tumor progression in oral squamous cell carcinoma.
abstract: OBJECTIVE
  KAI1 COOH-terminal interacting tetraspanin (KITENIN) contributes to tumor invasion and metastasis in various cancers. The aim of this study was to investigate expression of KITENIN in patients with oral cavity squamous cell carcinoma (SCC) and to determine whether KITENIN affects tumor cell behavior in oral cavity SCC cell line.
  
  
  METHODS
  Western blotting and immunohistochemistry was used to assess alteration of KITENIN expression in human oral cavity SCC and normal oral cavity mucosa. To evaluate the impact of KITENIN knockdown, the cell invasion assay and cell migration assay using small-interfering RNA were performed.
  
  
  RESULTS
  KITENIN protein expression was significantly increased in human oral cavity SCC tissues than in normal oral cavity mucosa by Western blotting. KITENIN immunoreactivity was strongly identified in human oral cavity SCC relative to adjacent normal tissue. Knockdown of KITENIN resulted in significantly reduced cell invasion in human oral cavity SCC cells (p=0.001). Cell migration showed a marked decrease in KITENIN knockdown oral cavity SCC cells compared to the negative control oral cavity SCC cells (p=0.01).
  
  
  CONCLUSIONS
  KITENIN is associated with tumor invasiveness and metastasis in human oral cavity SCC.
authors: Department of Otolaryngology-Head and Neck Surgery, Research Institute of Medical Sciences, Chonnam National University Medical School & Hwasun Hospital, Gwangju, Republic of Korea.
authors: Tae Mi
authors: TM
authors: Yoon
authors: Sun-Ae
authors: SA
authors: Kim
authors: Joon Kyoo
authors: JK
authors: Lee
authors: Young-Lan
authors: YL
authors: Park
authors: Gyu Yeol
authors: GY
authors: Kim
authors: Young-Eun
authors: YE
authors: Joo
authors: Jae Hyuk
authors: JH
authors: Lee
authors: Kyung Keun
authors: KK
authors: Kim
authors: Sang Chul
authors: SC
authors: Lim
mesh_terms: D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000556:secondary; D002352:Carrier Proteins / Q000378:metabolism* / Q000502:physiology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D054443:Cell Migration Assays; D003584:Cytological Techniques; D018450:Disease Progression; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism* / Q000502:physiology; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism* / Q000502:physiology
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C488287:VANGL1 protein, human