title: Monodisperse poly(lactide-co-glycolic acid)-based nanocarriers for gene transfection.
abstract: This contribution describes a simple, aerosol-based method for fabricating monodisperse particles containing mixtures of poly(lactide-co-glycolic acid) [PLGA], protamine sulfate (Prot), and poly(l-lysine) [PLL] as nanocarriers for gene transfection. Aqueous solutions of PLGA, Prot, and PLL were collison-atomized, and the resulting aerosolized droplets were dried "on the fly" to form solid particles, which then were electrostatically size-classified into 50, 100, and 200 nm mobility diameter samples. Measurements of cell viability and transfection reveal that the fabricated nanocarriers have a lower cytotoxicity (>85% in cell viability) and a higher transfection efficiency [>8.7 × 10(5)  in relative light units (RLU) mg(-1) ] than does 25 kDa polyethyleneimine (≈50% and 6.8 × 10(5)  RLU mg(-1) ).
authors: Department of Chemistry, Purdue University, IN 47907, USA.
authors: Jeong Hoon
authors: JH
authors: Byeon
authors: Hee-Kwon
authors: HK
authors: Kim
authors: Jeffrey T
authors: JT
authors: Roberts
mesh_terms: D004247:DNA / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D019344:Lactic Acid / Q000138:chemical synthesis*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000138:chemical synthesis*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014162:Transfection / Q000295:instrumentation*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D004247:DNA