title: Ameliorative effects of resveratrol on acute ovarian toxicity induced by total body irradiation in young adult rats.
abstract: OBJECTIVE
  The purpose of this study was to investigate the ovarian protective effects of resveratrol in rats exposed to total body irradiation.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Thirty female rats were randomized into four groups: (1) control group (n = 7); (2) low-dose (10 mg/kg) resveratrol group (n = 8); (3) high-dose (100 mg/kg) resveratrol group (n =7); and (4) sham irradiation group (n = 8). The drugs were administered intraperitoneally as single doses, and the rats were exposed to total body radiation 24 h after the treatment. The animals were sacrificed the following day, and their ovaries were excised for histopathological and biochemical analysis.
  
  
  METHODS
  The ovarian follicle counts were calculated, and irradiation-dependent ovarian damage and tissue levels of antioxidant enzymes were evaluated.
  
  
  RESULTS
  Group 2 and Group 3 showed significantly higher numbers of total follicle counts compared with Group 1 (P < 0.01). The low-dose resveratrol treatment was associated with significantly higher numbers of primary follicles than the high-dose group. The tissue activities of glutathione peroxidase (GsH-Px) and catalase (CAT) were significantly elevated in the resveratrol-treated animals. Evaluation of ovarian histology revealed no remarkable changes in fibrosis and leucocyte infiltration among the resveratrol-treated and control rats; however, vascularity was significantly reduced in the high-dose group (P = 0.014).
  
  
  CONCLUSIONS
  Resveratrol attenuated irradiation-dependent ovarian damage, suggesting that this natural antioxidant is effective in reducing the follicle loss induced by ionizing radiation.
authors: Inonu University Faculty of Medicine, Department of Obstetrics and Gynecology, Malatya, Turkey. dryavuzsimsek@gmail.com
authors: Yavuz
authors: Y
authors: Simsek
authors: Simay
authors: S
authors: Gurocak
authors: Yusuf
authors: Y
authors: Turkoz
authors: Nusret
authors: N
authors: Akpolat
authors: Onder
authors: O
authors: Celik
authors: Ali
authors: A
authors: Ozer
authors: Ercan
authors: E
authors: Yılmaz
authors: Ugur
authors: U
authors: Turhan
authors: Fatma
authors: F
authors: Ozyalin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D006080:Ovarian Follicle / Q000187:drug effects / Q000528:radiation effects; D010053:Ovary / Q000098:blood supply / Q000201:enzymology / Q000473:pathology / Q000528:radiation effects*; D011832:Radiation Injuries / Q000201:enzymology / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D018709:Statistics, Nonparametric; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D000077185:Resveratrol