title: Soluble plantain fibre blocks adhesion and M-cell translocation of intestinal pathogens.
abstract: Dietary fibres may have prebiotic effects mediated by promotion of beneficial bacteria. This study explores the possibility that soluble plant fibre may also improve health by inhibiting epithelial adhesion and translocation by pathogenic bacteria. We have focussed on soluble non-starch polysaccharide (NSP) from plantain bananas (Musa spp.) which previous studies showed to be particularly effective at blocking Escherichia coli epithelial adherence. In vitro and ex vivo studies assessed the ability of plantain NSP to inhibit epithelial cell adhesion and invasion of various bacterial pathogens, and to inhibit their translocation through microfold (M)-cells and human Peyer's patches mounted in Ussing chambers. Plantain NSP showed dose-related inhibition of epithelial adhesion and M-cell translocation by a range of pathogens. At 5mg/ml, a concentration readily achievable in the gut lumen, plantain NSP inhibited adhesion to Caco2 cells by Salmonella Typhimurium (85.0 ± 8.2%, P<.01), Shigella sonnei (46.6 ± 29.3%, P<.01), enterotoxigenic E.coli (56.1 ± 23.7%, P<.05) and Clostridium difficile (67.6 ± 12.3%, P<.001), but did not inhibit adhesion by enteropathogenic E.coli. Plantain NSP also inhibited invasion of Caco2 cells by S. Typhimurium (80.2 ± 9.7%) and Sh. sonnei (46.7 ± 13.4%); P<.01. Plantain NSP, 5mg/ml, also inhibited translocation of S. Typhimurium and Sh. sonnei across M-cells by 73.3 ± 5.2% and 46.4 ± 7.7% respectively (P<.05). Similarly, S. Typhimurium translocation across Peyer's patches was reduced 65.9 ± 8.1% by plantain NSP (P<.01). Soluble plantain fibre can block epithelial adhesion and M-cell translocation of intestinal pathogens. This represents an important novel mechanism by which soluble dietary fibres can promote intestinal health and prevent infective diarrhoea.
authors: Gastroenterology, Institute of Translational Medicine, University of Liverpool, L69 3GE Liverpool, UK.
authors: Carol L
authors: CL
authors: Roberts
authors: Asa V
authors: AV
authors: Keita
authors: Bryony N
authors: BN
authors: Parsons
authors: Maelle
authors: M
authors: Prorok-Hamon
authors: Paul
authors: P
authors: Knight
authors: Craig
authors: C
authors: Winstanley
authors: Niamh
authors: N
authors: O' Kennedy
authors: Johan D
authors: JD
authors: Söderholm
authors: Jonathan M
authors: JM
authors: Rhodes
authors: Barry J
authors: BJ
authors: Campbell
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001422:Bacterial Adhesion / Q000187:drug effects*; D018988:Bacterial Translocation / Q000187:drug effects*; D018938:Caco-2 Cells / Q000187:drug effects / Q000382:microbiology; D016360:Clostridioides difficile / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology; D004043:Dietary Fiber / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D028521:Musa / Q000737:chemistry*; D010581:Peyer's Patches / Q000187:drug effects / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology; D012764:Shigella sonnei / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology; D012995:Solubility