title: Photoluminescent and electrochemiluminescent dual-signaling probe for bio-thiols based on a ruthenium(II) complex.
abstract: Photoluminescence (PL) and electrochemiluminescence (ECL) detection techniques are highly sensitive and widely used methods for clinical diagnostics and analytical biotechnology. In this work, a unique ruthenium(II) complex, [Ru(bpy)(2)(DNBSO-bpy)](PF(6))(2) (bpy: 2,2'-bipyridine; DNBSO-bpy: 2,4-dinitrobenzenesulfonate of 4-(4-hydroxyphenyl)-2,2'-bipyridine), has been designed and synthesized as a highly sensitive and selective PL and ECL dual-signaling probe for the recognition and detection of bio-thiols in aqueous media. As a thiol-responsive probe, the complex can specifically and rapidly react with bio-thiols in aqueous solutions to yield a bipyridine-Ru(II) complex derivative, [Ru(bpy)(2)(HP-bpy)](2+) (HP-bpy: 4-(4-hydroxyphenyl)-2,2'-bipyridine), accompanied by the remarkable PL and ECL enhancements. The complex was used as a probe for the PL and ECL detections of cysteine (Cys) and glutathione (GSH) in aqueous solutions. The dose-dependent PL and ECL enhancements showed good linear relationships against the Cys/GSH concentrations with the detection limits at nano-molar concentration level. Moreover, the complex-loaded HeLa cells were prepared for PL imaging of the endogenous intracellular thiols. The results demonstrated the practical utility of the complex as a cell-membrane permeable probe for PL imaging detection of bio-thiols in living cells.
authors: State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116024, PR China. wenzhuzhang@yahoo.com.cn
authors: Wenzhu
authors: W
authors: Zhang
authors: Run
authors: R
authors: Zhang
authors: Jingmei
authors: J
authors: Zhang
authors: Zhiqiang
authors: Z
authors: Ye
authors: Dayong
authors: D
authors: Jin
authors: Jingli
authors: J
authors: Yuan
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D055664:Electrochemical Techniques; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008163:Luminescent Measurements; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D012428:Ruthenium / Q000737:chemistry*; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D013438:Sulfhydryl Compounds; D012428:Ruthenium