title: A sandwich assay for quantitative detection of transcription factors in cell lysate.
abstract: A double-stranded DNA (dsDNA) mediated sandwich assay was developed for quantitative detection of transcription factors. The detection limit for human recombinant c-jun protein is 2.5 ng, and for c-jun protein the limit is as low as 0.625 μg of cell lysate.
authors: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Chinese Academy of Sciences, Guangzhou, China.
authors: Zhiyuan
authors: Z
authors: Fang
authors: Wenjuan
authors: W
authors: Zhang
authors: Chenchen
authors: C
authors: Ge
authors: Jie
authors: J
authors: Liu
authors: Puchang
authors: P
authors: Lie
authors: Lingwen
authors: L
authors: Zeng
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D015336:Molecular Probe Techniques; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis*; D012680:Sensitivity and Specificity; D014157:Transcription Factors / Q000032:analysis*
chemical_list: D016755:Proto-Oncogene Proteins c-jun; D014157:Transcription Factors; D004247:DNA