title: Functionalization of poly-SNS-anchored carboxylic acid with Lys and PAMAM: surface modifications for biomolecule immobilization/stabilization and bio-sensing applications.
abstract: Poly(2-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl) (SNS) acetic acid) was electrochemically deposited on graphite electrodes and functionalized with lysine (Lys) amino acid and poly(amidoamine) derivatives (PAMAM G2 and PAMAM G4) to investigate their matrix properties for biosensor applications. Glucose oxidase (GOx) was immobilized onto the modified surface as the model enzyme. X-Ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) were used to report the surface properties of the matrices in each step of the biosensor construction. The biosensors were characterized in terms of their operational and storage stabilities and the kinetic parameters (K(app)(m) and I(max)). Three new glucose biosensors revealed good stability, featuring low detection limits (19.0 μM, 3.47 μM and 2.93 μM for lysine-, PAMAM G2- and PAMAM G4-functionalized electrodes, respectively) and prolonged the shelf lives (4, 5, and 6 weeks for Lys-, PAMAM G2- and PAMAM G4-modified electrodes, respectively). The proposed biosensors were tested for glucose detection on real human blood serum samples.
authors: Department of Polymer Science and Technology, Middle East Technical University, Ankara, Turkey.
authors: Sema
authors: S
authors: Demirci
authors: Fatma Bilge
authors: FB
authors: Emre
authors: Fulya
authors: F
authors: Ekiz
authors: Funda
authors: F
authors: Oğuzkaya
authors: Suna
authors: S
authors: Timur
authors: Cihangir
authors: C
authors: Tanyeli
authors: Levent
authors: L
authors: Toppare
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D002264:Carboxylic Acids / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D005947:Glucose / Q000032:analysis; D006801:Humans; D008239:Lysine / Q000737:chemistry; D018625:Microscopy, Atomic Force; D009757:Nylons / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011073:Polyamines / Q000737:chemistry; D013499:Surface Properties
chemical_list: D001786:Blood Glucose; D002264:Carboxylic Acids; D050091:Dendrimers; D004800:Enzymes, Immobilized; D009757:Nylons; C104700:PAMAM Starburst; C520550:PAMAM-G4; C531249:Poly(amidoamine); D011073:Polyamines; D005947:Glucose; D008239:Lysine