title: Preliminary experience on the use of the Adnatest® system for detection of circulating tumor cells in prostate cancer patients.
abstract: BACKGROUND
  The Adnatest® system combines immunomagnetic enrichment of epithelial cells with polymerase chain reaction for prostate cancer (PC)-specific transcripts for the detection circulating tumor cells (CTCs). We evaluated the Adnatest® in patients with castration-resistant PC receiving docetaxel chemotherapy.
  
  
  METHODS
  CTCs were assessed in 16 patients with castration-resistant PC before cycles one and three of chemotherapy. Furthermore, markers of stem cells and epithelial-mesenchymal transition were assessed. Treatment response was assessed by imaging and prostate-specific antigen measurements.
  
  
  RESULTS
  Before chemotherapy, 11 patients were Adnatest®-positive whereas five patients were Adnatest®-positive before cycle three. A positive Adnatest® correlated with radiological progression (p=0.02). Rates of disease progression in epidermal growth factor receptor (EGFR)-positive and -negative patients were 100% and 7.7% (p=0.03).
  
  
  CONCLUSIONS
  In this preliminary study, the Adnatest® detected CTCs in a considerable proportion of patients with castration-resistant PC. First data on certain markers (EGFR and aldehyd dehydrogenase 1) encourage future studies investigating transcripts predicting treatment response.
authors: Department of Urology, Eberhard-Karls University, Hoppe-Seyler Strasse 3, D-72076 Tübingen, Germany. tilman.todenhoefer@med.uni-tuebingen.de
authors: Tilman
authors: T
authors: Todenhöfer
authors: Jörg
authors: J
authors: Hennenlotter
authors: Susan
authors: S
authors: Feyerabend
authors: Stefan
authors: S
authors: Aufderklamm
authors: Johannes
authors: J
authors: Mischinger
authors: Ursula
authors: U
authors: Kühs
authors: Valentina
authors: V
authors: Gerber
authors: Jasmin
authors: J
authors: Fetisch
authors: David
authors: D
authors: Schilling
authors: Siegfried
authors: S
authors: Hauch
authors: Arnulf
authors: A
authors: Stenzl
authors: Christian
authors: C
authors: Schwentner
mesh_terms: D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use; D000077143:Docetaxel; D006801:Humans; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000097:blood* / Q000188:drug therapy / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D043823:Taxoids / Q000627:therapeutic use
chemical_list: D000970:Antineoplastic Agents; D012333:RNA, Messenger; D043823:Taxoids; D000077143:Docetaxel