title: SP-D polymorphisms and the risk of COPD.
abstract: BACKGROUND
  There are limited data linking serum levels of surfactant protein D, its genetic polymorphisms to the risk of Chronic Obstructive Pulmonary Disease (COPD).
  
  
  OBJECTIVE
  We sought to investigate these relationships using a case control study design.
  
  
  METHODS
  Post bronchodilator values of FEV1/FVC < 0.7 were used to diagnose COPD patients (n=115). Controls were healthy subjects with normal spirometry (n=106) Single nucleotide polymorphisms (rs721917, rs2243639, rs3088308) were genotyped using polymerase chain reaction (PCR) and restriction analysis. Serum SP-D levels were measured using a specific immunoassay.
  
  
  RESULTS
  Allele 'A' at rs3088308 (p < 0.00, B= -0.41) and 'C' allele at rs721917 (p=0.03; B= -0.30) were associated with reduced serum SP-D levels. Genotype 'T/T' at rs721917 was significantly associated with risk of COPD (p=0.01). Patients with repeat exacerbations had significantly higher serum SP-D even after adjusting for genetic factors.
  
  
  CONCLUSIONS
  We report for the first time that rs3088308 is an important factor influencing systemic SP-D levels and confirm the previous association of rs721917 to the risk of COPD and serum SP-D levels.
authors: University of Health Science, Lahore, Pakistan. drtaniashakoori@yahoo.com
authors: Tania A
authors: TA
authors: Shakoori
authors: Don D
authors: DD
authors: Sin
authors: S Nazim Hussain
authors: SN
authors: Bokhari
authors: Farkhanda
authors: F
authors: Ghafoor
authors: A R
authors: AR
authors: Shakoori
mesh_terms: D000328:Adult; D000368:Aged; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D010154:Pakistan / Q000453:epidemiology; D020641:Polymorphism, Single Nucleotide; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000453:epidemiology / Q000235:genetics*; D037663:Pulmonary Surfactant-Associated Protein D / Q000097:blood / Q000235:genetics*; D012307:Risk Factors
chemical_list: D037663:Pulmonary Surfactant-Associated Protein D