title: MicroRNA-9 suppresses uveal melanoma cell migration and invasion through             the NF-κB1 pathway.
abstract: The aggressive course of uveal melanoma is believed to reflect its unusually             invasive and metastatic nature, which is associated with the nuclear factor kappaB             (NF-κB) pathway. MicroRNAs (miRNAs) have been implicated in the regulation of             various biological and pathological processes in cancer, however, the special             role of miR-9 in uveal melanoma metastasis is largely unknown. In the present             study, we showed that miR-9 is significantly reduced in highly invasive uveal             melanoma cell lines, and suppressed migration and invasion of highly invasive             cells. Furthermore, miR-9 negatively modulated NF-κB1 expression by direct targeting             at its 3'-UTRs. Additionally, downstream targets of NF-κB1, such as MMP-2, MMP-9             and VEGFA, were regulated by miR-9 in the same pattern as NF-κB1. Therefore, miR-9             suppresses uveal melanoma cell migration and invasion partly through downregulation             of the NF-κB1 signaling pathway.
authors: Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu Province, PR China.
authors: Nannan
authors: N
authors: Liu
authors: Qingmin
authors: Q
authors: Sun
authors: Jie
authors: J
authors: Chen
authors: Jiaqi
authors: J
authors: Li
authors: Ying
authors: Y
authors: Zeng
authors: Sulan
authors: S
authors: Zhai
authors: Ping
authors: P
authors: Li
authors: Bin
authors: B
authors: Wang
authors: Xuerong
authors: X
authors: Wang
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D008545:Melanoma / Q000473:pathology*; D035683:MicroRNAs / Q000502:physiology*; D052002:NF-kappa B p50 Subunit / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D015398:Signal Transduction; D014604:Uveal Neoplasms / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D000098943:Uveal Melanoma
chemical_list: C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D052002:NF-kappa B p50 Subunit; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9