title: Pretransplant anti-HLA-Cw and anti-HLA-DP antibodies in sensitized patients.
abstract: We investigated the prevalence and the strength of anti-HLA-Cw and DP antibodies and clinical outcomes in kidney transplant recipients with isolated donor-specific anti-HLA-Cw antibodies. Patients on the waiting list were screened by Luminex single antigen beads (One Lambda). The strength of antibodies was determined by mean fluorescence intensity (MFI) values of the beads. Of the 1069 patients on the waiting list, 251 (24%) were sensitized with calculated panel reactive antibody >0%. The frequency and the median MFI values of anti-HLA antibodies to Cw (56%, 4955) and DP (35%, 2945) were lower than anti-HLA-A (79%, 10,194), B (86%, 11,235), DR (66%, 7866) and DQ (69%, 8283) (p<0.01). Among three major sensitizing events, only previous transplant was associated with development of all anti-HLA antibodies and history of pregnancy was associated only with development of anti-HLA-A antibodies. Eight patients with donor-specific anti-HLA-Cw antibodies received transplantation. During a median 6 months of follow-up (range 3-24 months), patient and graft survival was 100% without any acute rejection. In summary, the prevalence and the strength of anti-HLA-Cw and HLA-DP were lower compared to anti-HLA-A, B, DR, and DQ antibodies and previous organ transplantation was the main sensitizing event in our cohort of patients.
authors: Montefiore-Einstein Center for Transplantation, Albert Einstein College of Medicine, Bronx, NY 10467, USA.
authors: Min
authors: M
authors: Ling
authors: Kwaku
authors: K
authors: Marfo
authors: Peter
authors: P
authors: Masiakos
authors: Aws
authors: A
authors: Aljanabi
authors: Joel
authors: J
authors: Lindower
authors: Daniel
authors: D
authors: Glicklich
authors: Graciela
authors: G
authors: de Boccardo
authors: Stuart
authors: S
authors: Greenstein
authors: Javier
authors: J
authors: Chapochnick-Friedmann
authors: Liise
authors: L
authors: Kayler
authors: Milan
authors: M
authors: Kinkhabwala
authors: Enver
authors: E
authors: Akalin
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D015236:HLA-C Antigens / Q000276:immunology*; D006682:HLA-DP Antigens / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000097:blood / Q000276:immunology*; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D014019:Tissue Donors; D055815:Young Adult
chemical_list: D015236:HLA-C Antigens; D006682:HLA-DP Antigens; D007518:Isoantibodies