title: Chemical and biological properties of a highly branched β-glucan from edible mushroom Pleurotus sajor-caju.
abstract: Hot aqueous extraction of the basidiocarps of the mushroom Pleurotus sajor-caju provided a cold water-soluble, gel-like glucan, which was characterized chemically, and its effects on RAW 264.7 cell line (mouse leukaemic monocyte macrophage) activation were determined. NMR spectroscopy, HPSEC, methylation analysis, and a controlled Smith degradation showed it to have a branched structure with a (1→3)-linked β-Glcp main-chain, substituted at O-6 by single-unit β-Glcp side-chains, on the average of two to every third residues of the backbone, with a molar mass of 9.75 × 10(5) g mol(-1). In macrophage cell culture, the β-glucan induced production of NO and the cytokines TNF-α, IL-1β, these effects being very similar as those of Escherichia coli serotype 0111:B4 Sigma-Aldrich lipopolysaccharide (LPS), although not modifying the response of LPS-activated macrophages. The results suggest that the (1→3), (1→6)-linked β-glucan from P. sajor-caju may have potential for immunological activities, although additional experiments are necessary for a better understanding of the mechanisms involved.
authors: Departamento de Química, Universidade Federal de Goiás, Campus Catalão, 75704-020 Catalão GO, Brazil.
authors: Elaine R
authors: ER
authors: Carbonero
authors: Andrea Caroline
authors: AC
authors: Ruthes
authors: Cristina Setim
authors: CS
authors: Freitas
authors: Pillar
authors: P
authors: Utrilla
authors: Júlio
authors: J
authors: Gálvez
authors: Estefânia Viano
authors: EV
authors: da Silva
authors: Guilherme Lanzi
authors: GL
authors: Sassaki
authors: Philip Albert James
authors: PA
authors: Gorin
authors: Marcello
authors: M
authors: Iacomini
mesh_terms: D000363:Agaricales / Q000737:chemistry; D000818:Animals; D002240:Carbohydrate Sequence / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D051379:Mice; D008954:Models, Biological; D020076:Pleurotus / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D047071:beta-Glucans / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D047071:beta-Glucans