title: Control of cell adhesion and neurite outgrowth by patterned gold nanoparticles with tunable attractive or repulsive surface properties.
abstract: Guiding of neuronal cells on surfaces is required for the investigation of fundamental aspects of neurobiology, for tissue engineering, and for numerous bioelectronic applications. A modular method to establish nanostructured chemical templates for local deposition of gold nanoparticles is presented. A process comprising nanoimprint lithography, silanization, lift-off, and gold nanoparticle immobilization is used to fabricate the particle patterns. The chemical composition of the surface can be modified by in situ adsorption of cell-binding ligands to locally addressed particles. The versatility of this approach is demonstrated by inverting the binding affinity between rat cortical neurons and nanopatterned surfaces via wet-chemical means and thereby reversing the pattern of guided neurons.
authors: Peter Grünberg Institute, Forschungszentrum Jülich GmbH, 52428 Jülich, Germany.
authors: Sandra
authors: S
authors: Gilles
authors: Silke
authors: S
authors: Winter
authors: Kristin E
authors: KE
authors: Michael
authors: Simone H
authors: SH
authors: Meffert
authors: Pinggui
authors: P
authors: Li
authors: Kyrylo
authors: K
authors: Greben
authors: Ulrich
authors: U
authors: Simon
authors: Andreas
authors: A
authors: Offenhäusser
authors: Dirk
authors: D
authors: Mayer
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D006046:Gold / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D054802:Molecular Imprinting; D036103:Nanotechnology / Q000379:methods*; D016501:Neurites / Q000378:metabolism* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D014466:Ultraviolet Rays
chemical_list: D011092:Polyethylene Glycols; D006046:Gold