title: Neural stimulation on human bone marrow-derived mesenchymal stem cells by extremely low frequency electromagnetic fields.
abstract: Adult stem cells are considered multipotent. Especially, human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have the potential to differentiate into nerve type cells. Electromagnetic fields (EMFs) are widely distributed in the environment, and recently there have been many reports on the biological effects of EMFs. hBM-MSCs are weak and sensitive pluripotent stem cells, therefore extremely low frequency-electromagnetic fields (ELF-EMFs) could be affect the changes of biological functions within the cells. In our experiments, ELF-EMFs inhibited the growth of hBM-MSCs in 12 days exposure. Their gene level was changed and expression of the neural stem cell marker like nestin was decreased but the neural cell markers like MAP2, NEUROD1, NF-L, and Tau were induced. In immunofluorescence study, we confirmed the expression of each protein of neural cells. And also both oligodendrocyte and astrocyte related proteins like O4 and GFAP were expressed by ELF-EMFs. We suggest that EMFs can induce neural differentiation in BM-MSCs without any chemicals or differentiation factors.
authors: Dongguk University Research Institute of Biotechnology, Dongguk University, Seoul 100-715, Korea.
authors: Hyunjin
authors: H
authors: Cho
authors: Young-Kwon
authors: YK
authors: Seo
authors: Hee-Hoon
authors: HH
authors: Yoon
authors: Soo-Chan
authors: SC
authors: Kim
authors: Sung-Min
authors: SM
authors: Kim
authors: Kye-Yong
authors: KY
authors: Song
authors: Jung-Keug
authors: JK
authors: Park
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D001854:Bone Marrow Cells / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004574:Electromagnetic Fields; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D016875:tau Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D016875:tau Proteins; C093564:Neurogenic differentiation factor 1