title: Fibrinogen excretion in the urine and immunoreactivity in the kidney serves as a translational biomarker for acute kidney injury.
abstract: Fibrinogen (Fg) is significantly up-regulated in the kidney after acute kidney injury (AKI). We evaluated the performance of Fg as a biomarker for early detection of AKI. In rats and mice with kidney tubular damage induced by ischemia/reperfusion (I/R) or cisplatin administration, respectively; kidney tissue and urinary Fg increased significantly and correlated with histopathological injury, urinary kidney injury molecule-1 (KIM-1) and N-acetyl glucosaminidase (NAG) corresponding to the progression and regression of injury temporally. In a longitudinal follow-up of 31 patients who underwent surgical repair of abdominal aortic aneurysm, urinary Fg increased earlier than SCr in patients who developed postoperative AKI (AUC-ROC = 0.72). Furthermore, in a cohort of patients with biopsy-proven AKI (n = 53), Fg immunoreactivity in the tubules and interstitium increased remarkably and was able to distinguish patients with AKI from those without AKI (n = 59). These results suggest that immunoreactivity of Fg in the kidney, as well as urinary excretion of Fg, serves as a sensitive and early diagnostic translational biomarker for detection of AKI.
authors: Renal Division, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts, USA.
authors: Dana
authors: D
authors: Hoffmann
authors: Vanesa
authors: V
authors: Bijol
authors: Aparna
authors: A
authors: Krishnamoorthy
authors: Victoria R
authors: VR
authors: Gonzalez
authors: Gyorgy
authors: G
authors: Frendl
authors: Qin
authors: Q
authors: Zhang
authors: Peter L
authors: PL
authors: Goering
authors: Ronald P
authors: RP
authors: Brown
authors: Sushrut S
authors: SS
authors: Waikar
authors: Vishal S
authors: VS
authors: Vaidya
mesh_terms: D058186:Acute Kidney Injury / Q000150:complications / Q000175:diagnosis* / Q000473:pathology / Q000652:urine*; D000368:Aged; D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000601:surgery / Q000652:urine; D015415:Biomarkers / Q000652:urine; D002945:Cisplatin; D003710:Demography; D005260:Female; D005340:Fibrinogen / Q000235:genetics / Q000276:immunology* / Q000652:urine*; D006801:Humans; D007668:Kidney / Q000276:immunology* / Q000473:pathology*; D007684:Kidney Tubules / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009402:Nephrosis, Lipoid / Q000150:complications / Q000473:pathology / Q000652:urine; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000473:pathology / Q000652:urine; D057170:Translational Research, Biomedical; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D005340:Fibrinogen; D002945:Cisplatin