title: Application of Chemoenzymatic Hydrolysis in the Synthesis of 2-Monoacylglycerols.
abstract: The selective biocatalyzed synthesis of 2-monoacylglycerols (2-MAGs) through the use of commercially available immobilized Candida antarctica (Novozym435) and Rhizomucor miehei is explored. Reactions at room temperature result in the formation of a 2-MAG and a corresponding ethyl ester of the fatty acid with immobilized Candida antarctica within 2h with yields ranging from 36%-83%. Similar reaction conditions with immobilized Rhizomucor miehei yielded exclusively the 2-MAG after 24h with yields ranging from 37% to 88%. Yields vary on the acyl group at the sn-2 position and choice of enzyme involved.
authors: Center for Drug Discovery, 116 Mugar Hall, 360 Huntington Avenue, Northeastern University, Boston, MA 02115.
authors: Kyle M
authors: KM
authors: Whitten
authors: Alexandros
authors: A
authors: Makriyannis
authors: Subramanian K
authors: SK
authors: Vadivel