title: Genome characterization of a novel porcine bocavirus.
abstract: Using a high-throughput DNA sequencing method, one DNA sequence (contig01006), suspected to belong to a novel porcine bocavirus (PBoV), was found with a high rate of detection (19.6 %) in fecal samples from healthy piglets. Moreover, a novel PBoV (tentatively named PBoV3C) with a nearly complete genome sequence (5235 bp) was identified. PBoV3C exhibits typical genome characteristics of bocaviruses and shows the highest genomic sequence identity (78 % to 81 %) to PBoV3A/B (PBoV3/4-UK) and PBoV3D/E (PBoV3/4-HK), respectively. Phylogenetic and recombination analysis indicated high diversity, prevalence and complexity among the PBoVs. The phospholipase A2 (PLA2) site of VP1 and the secondary structure of VP2 of PBoV3C were also analyzed. Additionally, we propose a uniform method of PBoV nomenclature based on the VP1 gene.
authors: Department of Viral Diarrhea, National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Xuan-Wu District, Beijing 100052, China.
authors: Wan-zhu
authors: WZ
authors: Yang
authors: Jie-mei
authors: JM
authors: Yu
authors: Jin-song
authors: JS
authors: Li
authors: Wei-xia
authors: WX
authors: Cheng
authors: Can-ping
authors: CP
authors: Huang
authors: Zhao-jun
authors: ZJ
authors: Duan
mesh_terms: D000818:Animals; D052660:Bocavirus / Q000235:genetics* / Q000302:isolation & purification; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D017433:Protein Structure, Secondary; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D009626:Terminology as Topic; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins