title: Interactions of L-Arg with calf thymus DNA using neutral red dye as a fluorescence probe.
abstract: The interaction between l-Arg and calf thymus DNA (ctDNA) in sodium acetate-acetic acid buffer (pH=4) was investigated with the use of neutral red (NR) dye as a spectral probe coupled with UV-vis absorption, fluorescence, and circular dichroism (CD) spectroscopy technique. The UV absorption spectroscopy indicated that l-Arg interacted with ctDNA via electrostatic force and the fluorescence enhancing of the DNA-NR system verified the electrostatic interaction. In addition, detectable changes in the CD spectrum of ctDNA in the presence of l-Arg indicated conformational changes in the DNA double helix after interaction with the drug. Docking studies were found to corroborate the experimental results. All these results prove that this drug interacts with ctDNA via an electrostatic binding mode.
authors: Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, China-America CRC for Environment and Health, Shandong Province, Shandong University, 27# Shanda South Road, Jinan 250100, PR China.
authors: Jing
authors: J
authors: Lin
authors: Rutao
authors: R
authors: Liu
authors: Canzhu
authors: C
authors: Gao
mesh_terms: D000042:Absorption; D000818:Animals; D001120:Arginine / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D009499:Neutral Red / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005456:Fluorescent Dyes; D009499:Neutral Red; D004247:DNA; C427203:calf thymus DNA; D001120:Arginine