title: Type II thioesterase gene (ECO-orf27) from Amycolatopsis orientalis influences production of the polyketide antibiotic, ECO-0501 (LW01).
abstract: ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Complementation of the disrupted mutant with intact ECO-orf27 restored the production of LW01 suggesting that ECO-orf27 is crucial for LW01 biosynthesis. ECO-TE I, the gene encoding type I thioesterase from LW01 polyketide synthases, cannot complement ECO-orf27 deficient mutant distinguishing ECO-orf27 from type I thioesterase gene. Type II thioesterase gene pikAV from Streptomyces venezuelae could complement ECO-orf27 in A. orientalis indicating that the two genes are equivalent in their function. Overexpression of ECO-orf27 resulted in a 20 % increase in LW01 production providing an alternative approach for yield improvement.
authors: School of Biotechnology, East China University of Science and Technology, Meilong Rd. 130#, Shanghai, People's Republic of China.
authors: Yang
authors: Y
authors: Shen
authors: He
authors: H
authors: Huang
authors: Li
authors: L
authors: Zhu
authors: Minyu
authors: M
authors: Luo
authors: Daijie
authors: D
authors: Chen
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D064429:Fatty Acid Synthases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000378:metabolism; D055786:Gene Knockout Techniques; D005816:Genetic Complementation Test; D006146:Guanidines / Q000032:analysis* / Q000378:metabolism; D008969:Molecular Sequence Data; D005810:Multigene Family; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013869:Thiolester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C515838:13-hydroxy-2,12,14,16,22-pentamethyl-28-(N-methyl-guanidino)-octacosa-2,4,6,8,10,14,20,24-octaenoic acid (2-hydroxy-5-oxo-cyclopent-1-enyl)-amide; D001426:Bacterial Proteins; D005231:Fatty Acids, Unsaturated; D006146:Guanidines; D048630:Polyketide Synthases; D064429:Fatty Acid Synthases; D013869:Thiolester Hydrolases; C022738:thioesterase II