title: Dynamic high pressure microfluidization-assisted extraction and antioxidant activities of lentinan.
abstract: Dynamic high pressure microfluidization (DHPM) was applied to assist the lentinan extraction. Response surface methodology (RSM), based on Box-Behnken design, was employed to optimize the DHPM-assisted extraction conditions of lentinan. Three main independent variables (DHPM pressure, ratio of water to raw material, extraction temperature) were taken into consideration. A yield of 7.200% was obtained under a modified condition (ratio of water to raw material of 65 mL/g, DHPM pressure of 147 MPa, extraction temperature of 83 °C), which matched well with the predicted value of the model. The molecular weight of the DHPM-assisted extract and hot water extract was 913,329 and 965,361 Da, respectively. Compared to the traditional hot water extraction, the lentinan extracted by DHPM assisting had better scavenging capacity of hydroxyl radical, superoxide anion free radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and nitrite. It could be concluded that the DHPM was a promising method to enhance the yield and antioxidant activity of lentinan during extraction.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
authors: Xiaoqin
authors: X
authors: Huang
authors: Zongcai
authors: Z
authors: Tu
authors: Ying
authors: Y
authors: Jiang
authors: Hui
authors: H
authors: Xiao
authors: Qiuting
authors: Q
authors: Zhang
authors: Hui
authors: H
authors: Wang
mesh_terms: D032701:Biomimetics; D001713:Biphenyl Compounds / Q000737:chemistry; D005591:Chemical Fractionation / Q000295:instrumentation*; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism; D017665:Hydroxyl Radical / Q000737:chemistry; D007912:Lentinan / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D015233:Models, Statistical; D009573:Nitrites / Q000378:metabolism; D010851:Picrates / Q000737:chemistry; D011312:Pressure; D013481:Superoxides / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009573:Nitrites; D010851:Picrates; D014867:Water; D013481:Superoxides; D017665:Hydroxyl Radical; D007912:Lentinan; C004931:1,1-diphenyl-2-picrylhydrazyl