title: Dielectric spectroscopy for non-invasive monitoring of epithelial cell differentiation within three-dimensional scaffolds.
abstract: In this study, we introduce a cellular differentiation cellular model based on dielectric spectroscopy that characterizes epithelial differentiation processes. Non-invasive cellular monitoring was achieved within a three-dimensional microenvironment consisting of a cell-containing collagen I gel seeded onto microfabricated scaffolds. In this proof-of-concept investigation, Madin-Darby canine kidney cells were cultured within microfabricated, geometrically controlled scaffolds and allowed us to differentiate to hollow cyst-like structures. This transformation within the three-dimensional environment is monitored and characterized through dielectric spectroscopy while maintaining cell culture in vitro.
authors: Department of Biomedical Engineering, McGill University, Montreal, QC, Canada.
authors: Jamal
authors: J
authors: Daoud
authors: Koji
authors: K
authors: Asami
authors: Lawrence
authors: L
authors: Rosenberg
authors: Maryam
authors: M
authors: Tabrizian
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D058266:Dielectric Spectroscopy; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology*; D020342:Finite Element Analysis; D017629:Gap Junctions / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D054457:Tissue Scaffolds