title: Establishment of an experimental mouse model of trauma-hemorrhagic shock.
abstract: This study established an experimental mouse model of trauma-hemorrhagic shock (THS). THS-induced mice (C57BL/6J, n=33) were subjected to femoral fracture, ischemia for 90 min, and resuscitation for 15 min. The sham-operated mice (C57BL/6J, n=33) underwent the same anesthetic and surgical procedures, but neither trauma-hemorrhage nor fluid resuscitation were performed. Mean arterial pressure (MAP) and microvascular tissue perfusion over the small intestine, liver, and left kidney were longitudinally measured in all mice. Blood was collected for analysis at baseline and 3, 6, 12, and 24 h post resuscitation, and the small intestine, liver, and left kidney were resected for hematoxylin and eosin staining 24 h post resuscitation. Compared with the sham group, MAP and microvascular tissue perfusion over the small intestine, liver, and left kidney were all significantly reduced in the THS group at the end of hemorrhage. Following resuscitation, no significant differences were observed between the groups. THS induction was associated with significantly increased plasma concentrations of Cr, AST, CPK, IL-6, IL-10, and TNF-α from the baseline values by two- to three-fold after the hemorrhage phase, and THS-induced mice demonstrated significantly increased histological injury scores. The rapid drop in MAP and microvascular tissue perfusion observed following THS induction, and the gradual recovery post resuscitation, reflects the successful establishment of a THS experimental mouse model.
authors: Department of Hepatobiliary and Pancreatic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, PR China.
authors: Yin
authors: Y
authors: Tang
authors: Xue-Feng
authors: XF
authors: Xia
authors: Yun
authors: Y
authors: Zhang
authors: Bing-Feng
authors: BF
authors: Huang
authors: Tao
authors: T
authors: Ma
authors: Wei
authors: W
authors: Chen
authors: Ting-Bo
authors: TB
authors: Liang
mesh_terms: D000818:Animals; D062186:Arterial Pressure; D001774:Blood Chemical Analysis; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D005264:Femoral Fractures / Q000150:complications / Q000473:pathology / Q000628:therapy; D005440:Fluid Therapy; D006801:Humans; D007421:Intestine, Small / Q000098:blood supply / Q000503:physiopathology; D007511:Ischemia / Q000150:complications / Q000473:pathology / Q000628:therapy; D007552:Isotonic Solutions / Q000627:therapeutic use; D007668:Kidney / Q000098:blood supply / Q000503:physiopathology; D008099:Liver / Q000098:blood supply / Q000503:physiopathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000077325:Ringer's Lactate; D012771:Shock, Hemorrhagic / Q000209:etiology / Q000473:pathology* / Q000628:therapy*
chemical_list: D016207:Cytokines; D007552:Isotonic Solutions; D000077325:Ringer's Lactate