title: Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.
abstract: We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε(585 nm), 64,000 M(-1) x cm(-1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
authors: Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Victoria, Australia.
authors: Anne
authors: A
authors: Pettikiriarachchi
authors: Lan
authors: L
authors: Gong
authors: Matthew A
authors: MA
authors: Perugini
authors: Rodney J
authors: RJ
authors: Devenish
authors: Mark
authors: M
authors: Prescott
mesh_terms: D053148:Caspase 3 / Q000378:metabolism; D002845:Chromatography / Q000379:methods; D017931:DNA Primers / Q000235:genetics; D004789:Enzyme Activation; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation* / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008853:Microscopy / Q000379:methods; D016297:Mutagenesis, Site-Directed; D014461:Ultracentrifugation / Q000379:methods
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D053148:Caspase 3