title: SOX17 methylation inhibits its antagonism of Wnt signaling pathway in lung cancer.
abstract: The purpose of this study was to explore epigenetic changes and functions of SOX17 in human lung cancer. Five lung cancer cell lines and 88 primary lung cancer samples were examined in this study. Methylation-specific polymerase chain reaction (MSP), semi-quantitative reverse-transcription PCR, immunohistochemistry, luciferase reporter assays, colony-formation assays, and western blotting were used to analyze methylation changes and functions of SOX17 in lung cancer. SOX17 methylation was found in 60.2% of primary human lung cancer samples, and promoter region methylation of SOX17 silenced its expression. SOX17 methylation was associated with female patients and lung cancer differentiation. Colony-formation assays revealed that SOX17 suppressed lung cancer cell proliferation. Re-expression of SOX17 inhibited Wnt signaling in H23 lung cancer cell line. SOX17 acts as a Wnt signaling inhibitor.
authors: Department of Thoracic Surgery, Chinese PLA General Hospital, Beijing 100853, China.
authors: Dongtao
authors: D
authors: Yin
authors: Yan
authors: Y
authors: Jia
authors: Yuanzi
authors: Y
authors: Yu
authors: Malcolm V
authors: MV
authors: Brock
authors: James G
authors: JG
authors: Herman
authors: Chao
authors: C
authors: Han
authors: Xiaomo
authors: X
authors: Su
authors: Yang
authors: Y
authors: Liu
authors: Mingzhou
authors: M
authors: Guo
mesh_terms: D000368:Aged; D045744:Cell Line, Tumor; D002999:Clone Cells; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D055760:SOXF Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics; D014410:Tumor Stem Cell Assay; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: C109188:SOX17 protein, human; D055760:SOXF Transcription Factors; D051176:beta Catenin