title: Self-assembled diphenylalanine nanowires for cellular studies and sensor applications.
abstract: In this paper we present a series of experiments showing that vertical self-assembled diphenylalanine peptide nanowires (PNWs) are a suitable candidate material for cellular biosensing. We grew HeLa and PC12 cells onto PNW modified gold surfaces and observed no hindrance of cell growth caused by the peptide nanostructures; furthermore we studied the properties of PNWs by investigating their influence on the electrochemical behavior of gold electrodes. The PNWs were functionalized with polypyrrole (PPy) by chemical polymerization, therefore creating conducting peptide/polymer nanowire structures vertically attached to a metal electrode. The electroactivity of such structures was characterized by cyclic voltammetry. The PNW/PPy modified electrodes were finally used as amperometric dopamine sensors, yielding a detection limit of 3,1 microM.
authors: Technical University of Denmark, Department of Micro- and Nanotechnology, Ørsteds Plads 345ø, 2800 Kongens Lyngby, Denmark.
authors: Luigi
authors: L
authors: Sasso
authors: Indumathi
authors: I
authors: Vedarethinam
authors: Jenny
authors: J
authors: Emnéus
authors: Winnie E
authors: WE
authors: Svendsen
authors: Jaime
authors: J
authors: Castillo-León
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002455:Cell Division; D004151:Dipeptides; D004566:Electrodes; D006046:Gold; D006367:HeLa Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D053770:Nanowires; D016716:PC12 Cells; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D051381:Rats
chemical_list: D004151:Dipeptides; C026650:phenylalanylphenylalanine; D010649:Phenylalanine; D006046:Gold