title: An integrated chip for the high-throughput synthesis of transcription activator-like effectors.
abstract: A TALE of two assays: Transcription activator-like effectors (TALEs) are programmable proteins that can specifically recognize a DNA sequence. Previous strategies for the synthesis of TALEs were complicated and time-consuming. The solid-phase synthesis strategy demonstrated here allows quick and simple purification of the ligation product.
authors: Biomedical Engineering Department, College of Engineering, Peking University, Yan Nan Yuan 60, Beijing 100871, China.
authors: Zhao
authors: Z
authors: Wang
authors: Juan
authors: J
authors: Li
authors: Huang
authors: H
authors: Huang
authors: Gancheng
authors: G
authors: Wang
authors: Mingjun
authors: M
authors: Jiang
authors: Shenyi
authors: S
authors: Yin
authors: Changhong
authors: C
authors: Sun
authors: Hanshuo
authors: H
authors: Zhang
authors: Fengfeng
authors: F
authors: Zhuang
authors: Jianzhong Jeff
authors: JJ
authors: Xi
mesh_terms: D006367:HeLa Cells; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D015202:Protein Engineering; D015534:Trans-Activators / Q000138:chemical synthesis* / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000138:chemical synthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: D015534:Trans-Activators; D014157:Transcription Factors