title: Thermostable bacterial bioflocculant produced by Cobetia spp. isolated from Algoa Bay (South Africa).
abstract: A novel bioflocculant-producing bacteria was isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa and the effect of culture conditions on the bioflocculant production was investigated. Analysis of the partial nucleotide sequence of the 16S rDNA of the bacteria revealed 99% similarity to Cobetia sp. L222 and the sequence was deposited in GenBank as Cobetia sp. OAUIFE (accession number JF799092). Cultivation condition studies revealed that bioflocculant production was optimal with an inoculum size of 2% (v/v), initial pH of 6.0, Mn(2+) as the metal ion, and glucose as the carbon source. Metal ions, including Na(+), K(+), Li(+), Ca(2+)and Mg(2+) stimulated bioflocculant production, resulting in flocculating activity of above 90%. This crude bioflocculant is thermally stable, with about 78% of its flocculating activity remaining after heating at 100 °C for 25 min. Analysis of the purified bioflocculant revealed it to be an acidic extracellular polysaccharide.
authors: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa. ugbenyenanthony@gmail.com
authors: Anthony
authors: A
authors: Ugbenyen
authors: Sekelwa
authors: S
authors: Cosa
authors: Leonard
authors: L
authors: Mabinya
authors: Olubukola O
authors: OO
authors: Babalola
authors: Farhad
authors: F
authors: Aghdasi
authors: Anthony
authors: A
authors: Okoh
mesh_terms: D005427:Flocculation; D019015:Geologic Sediments / Q000382:microbiology; D044129:Halomonadaceae / Q000502:physiology*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D013019:South Africa