title: [Mesenchymal stromal cells synchronize the rhythm of protein synthesis under the effect of an exogenous signal].
abstract: A comparative study was performed of dense 5-hour cultures of rat hepatocytes and equal-density cultures of mesenchymal stromal cells (MSC) isolated from human adipose tissue of rat bone marrow. The cells were grown on collagen-coated class slides in serum-free medium. Unlike in hepatocytes, no rhythm of protein synthesis was initially revealed in MSC, but such a rhythm manifested itself when the culture medium was supplemented with melatonin (2 nM, 5 min). The results of experiments with cytoplasmic calcium chelator BAPTA-AM and protein kinase inhibitor H7 indicate that the mechanism of protein synthesis synchronization in MSC consists in calcium-dependent phosphorylation of cell proteins.
authors: V Ia
authors: VIa
authors: Brodskiĭ
authors: A V
authors: AV
authors: Vasil'ev
authors: V V
authors: VV
authors: Terskikh
authors: N D
authors: ND
authors: Zvezdina
authors: V I
authors: VI
authors: Fateeva
authors: L A
authors: LA
authors: Mal'chenko
authors: E V
authors: EV
authors: Kiseleva
authors: E I
authors: EI
authors: Bueverova
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D016895:Culture Media, Serum-Free; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D008550:Melatonin / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats
chemical_list: D002614:Chelating Agents; D016895:Culture Media, Serum-Free; D047428:Protein Kinase Inhibitors; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D004533:Egtazic Acid; D008550:Melatonin