title: Cellular and humoral immunity elicited by influenza vaccines in pediatric hematopoietic-stem cell transplantation.
abstract: Immunity induced by influenza vaccines following hematopoietic stem-cell transplantation (HSCT) is poorly understood. Here, 14 pediatric recipients (mean age: 6 years) received H1N1 (n=9) or H1N1/H3N2 (n=5) vaccines at a median of 5.7 months post-HSCT (HLA-identical related bone-marrow graft: 10/14). Fourteen clinically-matched non-vaccinated recipients were included as controls. Cellular response to vaccination was assessed by a T-cell proliferation assay. Humoral response was assessed by H1N1-specific antibody titration. IL2 and IFNγ responses to influenza were also evaluated by an intracellular cytokine accumulation method for some of the recipients. Higher proliferative responses to H1N1 (p=0.0001) and higher H1N1-specific antibody titers (p<0.02) were observed in vaccines opposed to non-vaccinated recipients. In some cases, proliferative responses to H1N1 developed while at the same time antibody titers did not reach protective (≥1:40) levels. Most recipients vaccinated with only the H1N1 strain had proliferative responses to both H1N1 and H3N2 (median stimulation index H1N1: 96, H3N2: 126 in responders). Finally, IL2 responses predominated over IFNγ responses (p<0.02) to influenza viruses in responders. In conclusion, H1N1 vaccination induced substantial cell-mediated immunity, and to a lesser extent, humoral immunity at early times post-HSCT. H1N1/H3N2 T-cell cross-reactivity and protective (IL2) rather than effector (IFNγ) cytokinic profiles were elicited.
authors: Laboratory of Immunology, Robert Debré Hospital, Assistance Publique-Hôpitaux de Paris, Paris 7 Denis Diderot University, Paris, France.
authors: Valérie
authors: V
authors: Guérin-El Khourouj
authors: Marie
authors: M
authors: Duchamp
authors: Anne
authors: A
authors: Krivine
authors: Béatrice
authors: B
authors: Pédron
authors: Marie
authors: M
authors: Ouachée-Chardin
authors: Karima
authors: K
authors: Yakouben
authors: Marie-Louise
authors: ML
authors: Frémond
authors: André
authors: A
authors: Baruchel
authors: Jean-Hugues
authors: JH
authors: Dalle
authors: Ghislaine
authors: G
authors: Sterkers
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000276:immunology; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007223:Infant; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008297:Male
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D007252:Influenza Vaccines