title: Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs.
abstract: BACKGROUND
  This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and a new scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs.
  
  
  METHODS
  The miniature pig was selected as an experimental model to compare the results in the left femoral bones (MSCs and CHC in scaffold transplantation into the iatrogenic partial distal growth plate defect) and right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. Bone marrow from os ilium as the source of MSCs was used. A porous cylinder consisting of 0.5% by weight type-I collagen and 30% by weight chitosan, was the optimal choice. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined.
  
  
  RESULTS
  Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases.
  
  
  CONCLUSIONS
  The promising results of this study reveal the new and effective means for the prevention of bone bridge formation after growth plate injury.
authors: Clinic of Pediatric Surgery, Orthopedics and Traumatology, Faculty Hospital Brno, Czech Republic. lplanka@seznam.cz
authors: Ladislav
authors: L
authors: Planka
authors: Robert
authors: R
authors: Srnec
authors: Petr
authors: P
authors: Rauser
authors: David
authors: D
authors: Stary
authors: Eva
authors: E
authors: Filova
authors: Josef
authors: J
authors: Jancar
authors: Jana
authors: J
authors: Juhasova
authors: Leos
authors: L
authors: Kren
authors: Alois
authors: A
authors: Necas
authors: Petr
authors: P
authors: Gal
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000254:growth & development; D048271:Chitosan; D019902:Chondrocytes / Q000637:transplantation*; D004838:Epiphyses / Q000254:growth & development; D005269:Femur / Q000254:growth & development / Q000601:surgery; D006132:Growth Plate / Q000254:growth & development*; D045164:Mesenchymal Stem Cell Transplantation; D057139:Nanofibers; D000072042:Salter-Harris Fractures; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D048271:Chitosan