title: Subcutaneous administration of rhIGF-I post irradiation exposure enhances hematopoietic recovery and survival in BALB/c mice.
abstract: It is unclear how to effectively mitigate against irradiation injury. In this study, we studied the capacity of recombinant human insulin-like growth factor-I (rhIGF-I) on hematologic recovery in irradiated BALB/c mice and its possible mechanism. BALB/c mice were injected with rhIGF-I subcutaneously at a dose of 100 μg/kg twice daily for 7 days after total body irradiation. Compared with a saline control group, treatment with rhIGF-I significantly improved the survival of mice after lethal irradiation (7.5 Gy). It was found that treatment with rhIGF-I not only could increase the frequency of Sca-1(+) cells in bone marrow harvested at Day 14 after irradiation, but also it could decrease the apoptosis of mononuclear cells induced by irradiation as measured by flow cytometry, suggesting that rhIGF-I may mediate its effects primarily through promoting hematopoietic stem cell/progenitor survival and protecting mononuclear cells from apoptosis after irradiation exposure. Moreover, we have found that rhIGF-I might facilitate thrombopoiesis in an indirect way. Our data demonstrated that rhIGF-I could promote overall hematopoietic recovery after ionizing radiation and reduce the mortality when administered immediately post lethal irradiation exposure.
authors: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury of PLA, Research Center of Nanomedicine of Chongqing, Third Military Medical University, Chongqing, China.
authors: Shilei
authors: S
authors: Chen
authors: Yang
authors: Y
authors: Xu
authors: Song
authors: S
authors: Wang
authors: Mingqiang
authors: M
authors: Shen
authors: Fang
authors: F
authors: Chen
authors: Mo
authors: M
authors: Chen
authors: Aiping
authors: A
authors: Wang
authors: Tianmin
authors: T
authors: Cheng
authors: Yongping
authors: Y
authors: Su
authors: Junping
authors: J
authors: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002470:Cell Survival; D005434:Flow Cytometry / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007279:Injections, Subcutaneous; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage*; D007963:Leukocytes, Mononuclear / Q000473:pathology / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011836:Radiation Tolerance; D011839:Radiation, Ionizing; D011994:Recombinant Proteins / Q000008:administration & dosage*; D034061:Thrombopoiesis / Q000187:drug effects / Q000528:radiation effects; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I