title: Correlating macrophage morphology and cytokine production resulting from biomaterial contact.
abstract: The morphological and inflammatory responses of adherent macrophages are correlated to evaluate the biocompatibility of surfaces. Monocyte-derived macrophage (MDM), THP-1, and THP-1 cells expressing GFP-actin chimeric protein were seeded onto glass, polyurethane (PU), and glass surface modified with quaternary ammonium salt functionalized chitosan (CH-Q) and hyaluronic acid (HA). Using confocal microscopy, the surface area, volume and 3D shape factor of adherent macrophages was quantified. For comparison, functional consequences of cell-surface interactions that activate macrophages and thereby elicit secretion of a proinflammatory cytokine were evaluated. Using an enzyme linked immune sorbent assay, tumor necrosis factor-alpha (TNF-α) was measured. On glass, macrophages exhibited mainly an amoeboid shape, exhibited the largest surface area, volume, and 3D shape factor and produced the most TNF-α. On PU, macrophages displayed mainly a hemispherical shape, exhibited an intermediate volume, surface area and 3D shape factor, and produced moderate TNF-α. In contrast, on CH-Q and HA surfaces, macrophages were spherical, exhibited the smallest volume, surface area, and 3D shape factor, and produced the least TNF-α. These studies begin to validate the use of GFP-actin-modified MDM as a novel tool to correlate cell morphology with inflammatory cell response.
authors: Department of Anesthesiology and Critical Care, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6802, USA.
authors: Hyun-Su
authors: HS
authors: Lee
authors: Stanley J
authors: SJ
authors: Stachelek
authors: Nancy
authors: N
authors: Tomczyk
authors: Matthew J
authors: MJ
authors: Finley
authors: Russell J
authors: RJ
authors: Composto
authors: David M
authors: DM
authors: Eckmann
mesh_terms: D000199:Actins / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects*; D048429:Cell Size / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional; D016086:Lentivirus / Q000235:genetics; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects; D018613:Microscopy, Confocal; D009000:Monocytes / Q000166:cytology; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013499:Surface Properties; D014161:Transduction, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D000199:Actins; D001672:Biocompatible Materials; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; D049452:Green Fluorescent Proteins