title: Cell therapy with adipose tissue-derived stem/stromal cells for elastase-induced pulmonary emphysema in rats.
abstract: OBJECTIVE
  The purpose of this study was to elucidate the mechanism underlying the effects of adipose tissue-derived stem/stromal cell (ASC) transplantation on porcine pancreatic elastase-induced emphysema.
  
  
  METHODS
  ASCs (2.5 × 10(6)) were transplanted into pancreatic elastase (250 U/kg)-treated rats, after which gas exchange and growth factor/cytokine levels in lung tissue were determined.
  
  
  RESULTS
  ASC transplantation restored pulmonary function (arterial oxygen tension and alveolar-arterial oxygen tension difference) almost to that of normal animals. Enlargement of the alveolar airspaces was inhibited. HGF and CINC-1 levels were significantly higher in the ASC group even at 2 weeks after transplantation. Sponge implantation with CINC-1 induced neovascular formation with increased HGF. In vitro secretion of HGF and CINC-1 from ASCs was promoted in the presence of IL-1β.
  
  
  CONCLUSIONS
  Not only HGF, but also CINC-1, secreted from transplanted and viable ASCs presumably contributed to lung repair through angiogenesis.
authors: Division of Respiratory & Infectious Diseases, Department of Internal Medicine, St Marianna University School of Medicine, Kawasaki, Japan.
authors: Naoki
authors: N
authors: Furuya
authors: Mitsuko
authors: M
authors: Takenaga
authors: Yuki
authors: Y
authors: Ohta
authors: Yukie
authors: Y
authors: Tokura
authors: Akemi
authors: A
authors: Hamaguchi
authors: Aya
authors: A
authors: Sakamaki
authors: Hirotaka
authors: H
authors: Kida
authors: Hiroshi
authors: H
authors: Handa
authors: Hiroki
authors: H
authors: Nishine
authors: Masamichi
authors: M
authors: Mineshita
authors: Teruomi
authors: T
authors: Miyazawa
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D054360:Chemokine CXCL1 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005740:Gases / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D018919:Neovascularization, Physiologic; D010196:Pancreatic Elastase; D019919:Prosthesis Implantation; D011656:Pulmonary Emphysema / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D051381:Rats; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000637:transplantation; D034421:Sus scrofa
chemical_list: D054360:Chemokine CXCL1; C513297:Cxcl1 protein, rat; D016207:Cytokines; D005740:Gases; D036341:Intercellular Signaling Peptides and Proteins; D010196:Pancreatic Elastase