title: Negative-pressure cavitation extraction of four main vinca alkaloids from Catharanthus roseus leaves.
abstract: In the present study, an improved method termed negative-pressure cavitation extraction (NPCE) followed by reverse phase high-performance liquid chromatography (RP-HPLC) was developed for the extraction and quantification of vindoline (VDL), catharanthine (CTR), vincristine (VCR) and vinblastine (VLB) from Catharanthus roseus leaves. The optimized method employed 60-mesh particles, 80% ethanol, a negative pressure of -0.075 MPa, a solid to liquid ratio of 1:20, 30 min of extraction and three extraction cycles. Under these optimized conditions, the extraction yields of VDL, CTR, VCR and VLB are 0.5783, 0.2843, 0.018 and 0.126 mg/g DW, respectively. These extraction yields are equivalent to those from the well-known ultrasonic extraction method and higher than the yields from maceration extraction and heating reflux extraction. Our results suggest that NPCE-RP-HPLC represents an excellent alternative for the extraction and quantification of vinca alkaloids for pilot- and industrial-scale applications.
authors: Engineering Research Center of Forestry Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
authors: Fansong
authors: F
authors: Mu
authors: Liuqing
authors: L
authors: Yang
authors: Wei
authors: W
authors: Wang
authors: Meng
authors: M
authors: Luo
authors: Yujie
authors: Y
authors: Fu
authors: Xiaorui
authors: X
authors: Guo
authors: Yuangang
authors: Y
authors: Zu
mesh_terms: D030008:Catharanthus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D000431:Ethanol / Q000737:chemistry; D010316:Particle Size; D010936:Plant Extracts / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry*; D052616:Solid Phase Extraction; D012997:Solvents; D050257:Tubulin Modulators / Q000302:isolation & purification; D014618:Vacuum; D014747:Vinblastine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D014748:Vinca Alkaloids / Q000302:isolation & purification*; D014750:Vincristine / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D012997:Solvents; D050257:Tubulin Modulators; D014748:Vinca Alkaloids; D000431:Ethanol; C009667:vindoline; D014750:Vincristine; D014747:Vinblastine; C017836:catharanthine