title: Integrin-linked kinase (ILK) expression correlates with tumor severity in clear cell renal carcinoma.
abstract: Integrin-linked kinase (ILK) is an unique intracellular serine/threonine kinase and adapter protein. When dysregulated, it has been associated with increased cell proliferation, anchorage-independent cell growth, evasion of apoptosis, angiogenesis, invasion of surrounding tissues, downregulation of E-cadherin expression, nuclear translocation of β-catenin and metastasis, all features of tumoral malignancy. The objective of the present work was to evaluate the expression of ILK in clear cell renal carcinomas (CCRC) as a possible prognostic indicator. ILK immunoexpression was evaluated in a tissue microarray (TMA) with 45 human CCRCs. In addition, the apoptotic and proliferative indices and the immuno-expression of β-catenin and E-cadherin were also evaluated. E-cadherin expression was significantly decreased in tumors with positive ILK expression in relation to those with negative immunoexpression (p = 0.011). ILK immunostaining was significantly increased in high-grade in comparison to low-grade CCRCs (p = 0.0008). ILK expression was also associated with increased proliferative index (p = 0.020), tumor size >7.0 cm (p = 0.018) and with renal vein and capsule invasion (p = 0.003 and p = 0.00). Finally, tumors stage I and II (noninvasive) presented significantly reduced ILK immunoexpression when compared to stage III (locally invasive) (p = 0.0028). ILK immunoexpression in CCRC increases with loss of intercellular adhesion, nuclear grading, increased proliferative index and Robson stage. Altogether, our data suggest a possible role for ILK in the progression of CRCC.
authors: Faculdade de Ciências da Saúde Dr José Antonio Garcia Coutinho, Universidade do Vale do Sapucai, Avenida Alfredo Custódio de Paula 360, CEP 37550000, Pouso Alegre, Minas Gerais, Brazil. mi.engelman@uol.com.br
authors: Míriam de Fátima Brasil
authors: Mde F
authors: Engelman
authors: Rogério Mendes
authors: RM
authors: Grande
authors: Marcelo Andery
authors: MA
authors: Naves
authors: Marcello Fabiano
authors: MF
authors: de Franco
authors: Vicente
authors: V
authors: de Paulo Castro Teixeira
mesh_terms: D017209:Apoptosis / Q000502:physiology; D015820:Cadherins / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000737:chemistry / Q000201:enzymology* / Q000473:pathology*; D048708:Cell Growth Processes / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology*; D060787:Neoplasm Grading; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000378:metabolism; D018709:Statistics, Nonparametric; D046888:Tissue Array Analysis; D051176:beta Catenin / Q000378:metabolism; D000099200:Scaffold Protein ILK
chemical_list: D015820:Cadherins; D017346:Protein Serine-Threonine Kinases; D051176:beta Catenin; D000099200:Scaffold Protein ILK