title: Structural characterization of neutral and acidic glycolipids from Thermus thermophilus HB8.
abstract: The structural characterization of glycolipids from Thermus thermophilus HB8 was performed in this study. Two neutral and one acidic glycolipids were extracted and purified by the modified TLC-blotting method, after which their chemical structures were determined by chemical composition analysis, mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. The structure of one of the neutral glycolipids, NGL-A, was Galp(α1-6)GlcpNacyl(β1-2)Glcp(α1-)acyl(2)Gro, and the other, NGL-C, was Galf(β1-2)Galp(α1-6)GlcpNacyl(β1-2)Glcp(α1-)acyl(2)Gro. The structure of NGL-C was identical to that reported previously [Oshima, M. and Ariga, T. (1976) FEBS Lett. 64, 440]. Both neutral glycolipids shared a common structural unit found in the Thermus species. The acyl groups found in NGL-A and NGL-C, iso-type pentadecanoxy and heptadecanoxy fatty acid, were also the same as those found in this species. In contrast, the acidic glycolipid, AGL-B, possessed the structure of N-(((GlcpNAc(α1-)acyl(2)Gro)P-2)GroA)alkylamine. The alkyl group in AGL-B was an iso-type heptadecanyl, suggesting that the iso-type structure of the long alkyl chain is responsible for the thermal stability of the bacteria.
authors: Department of Chemistry, Biotechnology and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, Kagoshima, Japan. ysuda@eng.kagoshima-u.ac.jp
authors: Yasuo
authors: Y
authors: Suda
authors: Fumiaki
authors: F
authors: Okazaki
authors: Yushi
authors: Y
authors: Hasegawa
authors: Seiji
authors: S
authors: Adachi
authors: Koichi
authors: K
authors: Fukase
authors: Susumu
authors: S
authors: Kokubo
authors: Seiki
authors: S
authors: Kuramitsu
authors: Shoichi
authors: S
authors: Kusumoto
mesh_terms: D002240:Carbohydrate Sequence; D002725:Chloroform / Q000737:chemistry; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D008969:Molecular Sequence Data; D013696:Temperature; D016963:Thermus thermophilus / Q000737:chemistry*
chemical_list: D006017:Glycolipids; D002725:Chloroform; D000432:Methanol