title: LINE-1 retrotransposition events regulate gene expression after X-ray irradiation.
abstract: Long interspersed nuclear element-1 (LINE-1) retrotransposons are mobile elements that insert into new genomic locations via reverse transcription of an RNA intermediate. The mechanism of retrotransposition is not entirely understood. The integration of these elements occurs by target-primed reverse transcription (TPRT), which initiates double-strand breaks (DSBs) during the LINE-1 integration. Also, X-ray is known to induce DNA damage. The aim of this study was to evaluate the potential effects of LINE-1 de novo retrotransposition on the expression of different genes after X-ray irradiation in human endothelial cells. After stable transfection of the human hybrid endothelial cell line EA.hy926 with the human LINE-1 element, we analyzed the expression of different genes after irradiation with 5 Gy X-rays by reverse transcription-polymerase chain reaction (RT-PCR). We determine the expression level of phosphorylated p53 and γ-histone H2AX protein levels upon X-ray irradiation with 5 Gy for 24 h. Our results showed that EA.hy926 LINE-1 cell clones react with a strong upregulation of phosphorylated p53 protein, already 15 min after irradiation compared to the wild type (WT) cells. Also, the expression of γ-histone H2AX protein was elevated in the cell clones with retrotransposition events 15 min after irradiation, whereas the WT cells have a delayed expression of phosphorylated histone H2AX protein. Taken together, our findings provide that LINE-1 retrotransposition events regulate different gene expression after irradiation in the EA.hy926 cell line.
authors: Institute of Anatomy, University Hospital Essen, Essen, Germany. ferya.banaz-yasar@uk-essen.de
authors: Ferya
authors: F
authors: Banaz-Yaşar
authors: Nilgün
authors: N
authors: Gedik
authors: Selda
authors: S
authors: Karahan
authors: David
authors: D
authors: Diaz-Carballo
authors: Birthe M
authors: BM
authors: Bongartz
authors: Süleyman
authors: S
authors: Ergün
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D002999:Clone Cells; D053903:DNA Breaks, Double-Stranded / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics* / Q000528:radiation effects*; D010766:Phosphorylation / Q000528:radiation effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D014965:X-Rays / Q000009:adverse effects
chemical_list: D016159:Tumor Suppressor Protein p53