title: Analytical challenges in the detection of peptide hormones for anti-doping purposes.
abstract: Although significant progress has been achieved during the past few years with the introduction of new assays and analytical methodologies, the detection and quantification of protein analytes, in particular of peptide hormones, continues to pose analytical challenges for the World Anti-Doping Agency-accredited anti-doping laboratories. In this article, the latest achievements in the application of MS-based methodologies and specific biochemical and immunological assays to detect some of the prohibited substances listed in section S2 of the World Anti-Doping Agency List of Prohibited Substances and Methods are reviewed. In addition, we look towards the future by focusing on some of the most promising analytical approaches under development for the detection of so-called 'biomarkers of doping'.
authors: World Anti-Doping Agency, 800 Place Victoria, Montreal, Quebec, H4Z 1B7, Canada. osquel.barroso@WADA-ama.org
authors: Osquel
authors: O
authors: Barroso
authors: David J
authors: DJ
authors: Handelsman
authors: Christian
authors: C
authors: Strasburger
authors: Mario
authors: M
authors: Thevis
mesh_terms: D045930:Anabolic Agents / Q000097:blood; D056352:Athletes; D015415:Biomarkers / Q000097:blood; D004282:Documentation; D004300:Doping in Sports; D006397:Hematinics / Q000097:blood; D019382:Human Growth Hormone / Q000097:blood; D006801:Humans; D007390:International Agencies; D013058:Mass Spectrometry / Q000379:methods; D055432:Metabolomics / Q000379:methods; D036361:Peptide Hormones / Q000097:blood*; D058573:Performance-Enhancing Substances / Q000097:blood*; D040901:Proteomics / Q000379:methods; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D045930:Anabolic Agents; D015415:Biomarkers; D006397:Hematinics; D036361:Peptide Hormones; D058573:Performance-Enhancing Substances; D019382:Human Growth Hormone