title: RNA polymerase II transcriptional silencing in growing and fully grown germinal vesicle oocytes isolated from gonadotropin-stimulated and non-stimulated gilts.
abstract: Global transcription silencing occurs in the oocyte during its final phase of growth. The particular mechanism of this silencing is not well understood. Here, we investigated the silencing of RNA polymerase II transcription in porcine oocytes. First, we investigated the transcriptional activity of germinal vesicle oocytes derived from stimulated and non-stimulated gilts, but no transcriptional activity was observed. Second, we focused on the fate of RNA polymerase II in growing and fully grown oocytes. Active and inactive forms of RNA polymerase II were detected in growing oocytes by immunofluorescence and Western blots. In contrast, only the inactive form of RNA polymerase II was detected in fully grown oocytes. To evaluate if the inactive form of RNA polymerase II is released from DNA, the oocytes were subsequently permeabilized and fixed in one step. After this modified fixation protocol, the immunofluorescent labeling was negative in fully grown oocytes, but remained unchanged (positive) in growing oocytes. These results indicate that the inactive form of RNA polymerase II is not bound to DNA during the oocyte growth. Finally, based on Western blot analysis of different stages of oocyte maturation, the inactive form of RNA polymerase II was detected in metaphase I but not in metaphase II. Our study confirmed the global transcription silencing of fully grown oocytes. Compared with other mammalian species (e.g., mouse), the mechanism of RNA polymerase II silencing in porcine oocytes seems to be similar, despite some differences in dynamics.
authors: Department of Biology of Reproduction, Institute of Animal Science, Prague, Czech Republic. barnetova.irena@vuzv.cz
authors: Irena
authors: I
authors: Barnetova
authors: Martin
authors: M
authors: Morovic
authors: Frantisek
authors: F
authors: Strejcek
authors: Olga
authors: O
authors: Østrup
authors: Poul
authors: P
authors: Hyttel
authors: Heiner
authors: H
authors: Niemann
authors: Jozef
authors: J
authors: Laurincik
authors: Josef
authors: J
authors: Fulka
authors: Helena
authors: H
authors: Fulka
mesh_terms: D000241:Adenosine / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D005260:Female; D020868:Gene Silencing; D006062:Gonadotropins / Q000378:metabolism; D007150:Immunohistochemistry; D007553:Isotope Labeling; D051379:Mice; D009865:Oocytes / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D010766:Phosphorylation; D012319:RNA Polymerase II / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D013552:Swine; D014158:Transcription, Genetic; D014529:Uridine / Q000737:chemistry / Q000378:metabolism
chemical_list: D006062:Gonadotropins; D012319:RNA Polymerase II; D000241:Adenosine; D014529:Uridine