title: Analysis of solvent tolerance in Pseudomonas putida DOT-T1E based on its genome sequence and a collection of mutants.
abstract: Pseudomonas putida strains are prevalent in a variety of pristine and polluted environments. The genome of the solvent-tolerant P. putida strain DOT-T1E which thrives in the presence of high concentrations of monoaromatic hydrocarbons, contains a circular 6.3 Mbp chromosome and a 133 kbp plasmid. Omics information has been used to identify the genes and proteins involved in solvent tolerance in this bacterium. This strain uses a multifactorial response that involves fine-tuning of lipid fluidity, activation of a general stress-response system, enhanced energy generation, and induction of specific efflux pumps that extrude solvents to the medium. Local and global transcriptional regulators participate in a complex network of metabolic functions, acting as the decision makers in the response to solvents.
authors: Consejo Superior de Investigaciones Científicas, EEZ, Department of Environmental Protection, 18008 Granada, Spain.
authors: Zulema
authors: Z
authors: Udaondo
authors: Estrella
authors: E
authors: Duque
authors: Matilde
authors: M
authors: Fernández
authors: Lázaro
authors: L
authors: Molina
authors: Jesús
authors: J
authors: de la Torre
authors: Patricia
authors: P
authors: Bernal
authors: José-Luis
authors: JL
authors: Niqui
authors: Cecilia
authors: C
authors: Pini
authors: Amalia
authors: A
authors: Roca
authors: Miguel A
authors: MA
authors: Matilla
authors: M Antonia
authors: MA
authors: Molina-Henares
authors: Hortencia
authors: H
authors: Silva-Jiménez
authors: Gloria
authors: G
authors: Navarro-Avilés
authors: Andreas
authors: A
authors: Busch
authors: Jesús
authors: J
authors: Lacal
authors: Tino
authors: T
authors: Krell
authors: Ana
authors: A
authors: Segura
authors: Juan-Luis
authors: JL
authors: Ramos
mesh_terms: D016680:Genome, Bacterial; D009154:Mutation; D016958:Pseudomonas putida / Q000187:drug effects* / Q000235:genetics; D012997:Solvents
chemical_list: D012997:Solvents