title: [Standard algorithm of molecular typing of Yersinia pestis strains].
abstract: OBJECTIVE
  Development of the standard algorithm of molecular typing of Yersinia pestis that ensures establishing of subspecies, biovar and focus membership of the studied isolate. Determination of the characteristic strain genotypes of plague infectious agent of main and nonmain subspecies from various natural foci of plague of the Russian Federation and the near abroad.
  
  
  METHODS
  Genotyping of 192 natural Y. pestis strains of main and nonmain subspecies was performed by using PCR methods, multilocus sequencing and multilocus analysis of variable tandem repeat number.
  
  
  RESULTS
  A standard algorithm of molecular typing of plague infectious agent including several stages of Yersinia pestis differentiation by membership: in main and nonmain subspecies, various biovars of the main subspecies, specific subspecies; natural foci and geographic territories was developed. The algorithm is based on 3 typing methods--PCR, multilocus sequence typing and multilocus analysis of variable tandem repeat number using standard DNA targets--life support genes (terC, ilvN, inv, glpD, napA, rhaS and araC) and 7 loci of variable tandem repeats (ms01, ms04, ms06, ms07, ms46, ms62, ms70). The effectiveness of the developed algorithm is shown on the large number of natural Y. pestis strains. Characteristic sequence types of Y. pestis strains of various subspecies and biovars as well as MLVA7 genotypes of strains from natural foci of plague of the Russian Federation and the near abroad were established.
  
  
  CONCLUSIONS
  The application of the developed algorithm will increase the effectiveness of epidemiologic monitoring of plague infectious agent, and analysis of epidemics and outbreaks of plague with establishing the source of origin of the strain and routes of introduction of the infection.
authors: G A
authors: GA
authors: Eroshenko
authors: G N
authors: GN
authors: Odinokov
authors: L M
authors: LM
authors: Kukleva
authors: A I
authors: AI
authors: Pavlova
authors: Ia M
authors: IaM
authors: Krasnov
authors: N Iu
authors: NIu
authors: Shavina
authors: N P
authors: NP
authors: Guseva
authors: N A
authors: NA
authors: Vinogradova
authors: V V
authors: VV
authors: Kutyrev
mesh_terms: D000465:Algorithms; D017931:DNA Primers; D014644:Genetic Variation; D016680:Genome, Bacterial; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats / Q000235:genetics*; D058885:Multilocus Sequence Typing / Q000379:methods; D010802:Phylogeny; D010930:Plague / Q000175:diagnosis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012426:Russia; D013045:Species Specificity; D015010:Yersinia pestis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015011:Yersinia pseudotuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015012:Yersinia pseudotuberculosis Infections / Q000175:diagnosis / Q000382:microbiology
chemical_list: D017931:DNA Primers