title: Cell viability of microencapsulated Bifidobacterium animalis subsp. lactis under freeze-drying, storage and gastrointestinal tract simulation conditions.
abstract: The purpose of this study was to improve the survival of Bifidobacterium animalis subsp. lactis 10140 during freeze-drying process by microencapsulation, using a special pediatric prebiotics mixture (galactooligosaccharides and fructooligosaccharides). Probiotic microorganisms were encapsulated with a coat combination of prebiotics-calcium-alginate prior to freeze-drying. Both encapsulated and free cells were then freeze-dried in their optimized combinations of skim milk and prebiotics. Response surface methodology (RSM) was used to produce a coating combination as well as drying medium with the highest cell viability during freeze-drying. The optimum encapsulation composition was found to be 2.1 % Na-alginate, 2.9 % prebiotic, and 21.7 % glycerol. Maximum survival predicted by the model was 81.2 %. No significant (p > 0.05) difference between the predicted and experimental values verified the adequacy of final reduced models. The protection ability of encapsulation was then examined over 120 days of storage at 4 and 25 °C and exposure to a sequential model of infantile GIT conditions including both gastric conditions (pH 3.0 and 4.0, 90 min, 37 °C) and intestinal conditions (pH 7.5, 5 h, 37 °C). Significantly improved cell viability showed that microencapsulation of B. lactis 10140 with the prebiotics was successful in producing a stable symbiotic powdery nutraceutical.
authors: Department of Microbiology, University Putra Malaysia, Selangor, Malaysia.
authors: Fatemeh
authors: F
authors: Shamekhi
authors: Mustafa
authors: M
authors: Shuhaimi
authors: Arbakariya
authors: A
authors: Ariff
authors: Yazid A
authors: YA
authors: Manap
mesh_terms: D001644:Bifidobacterium / Q000502:physiology* / Q000528:radiation effects; D015169:Colony Count, Microbial; D004339:Drug Compounding; D004356:Drug Storage / Q000379:methods; D005612:Freeze Drying; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007223:Infant; D041943:Infant Formula; D050296:Microbial Viability / Q000528:radiation effects*; D019936:Probiotics / Q000528:radiation effects*; D013696:Temperature; D013997:Time Factors