title: Ultrasound-assisted extraction of syringin from the bark of Ilex rotunda thumb using response surface methodology.
abstract: In this work, a rapid extraction method based on ultrasound-assisted extraction (UAE) of syringin from the bark of Ilex rotunda Thumb using response surface methodology (RSM) is described. The syringin was analyzed and quantified by high performance liquid chromatography coupled with UV detection (HPLC-UV). The extraction solvent, extraction temperature and extraction time, the three main factors for UAE, were optimized with Box-Behnken design (BBD) to obtain the highest extraction efficiency. The optimal conditions were the use of a sonication frequency of 40 kHz, 65% methanol as the solvent, an extraction time of 30 min and an extraction temperature of 40 °C. Using these optimal conditions, the experimental values agreed closely with the predicted values. The analysis of variance (ANOVA) indicated a high goodness of model fit and the success of the RSM method for optimizing syringin extraction from the bark of I. rotunda.
authors: College of Pharmacy, Hebei University, Baoding 071002, China.|The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China.
authors: Li-Chun
authors: LC
authors: Zhao
authors: The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China.
authors: Ying
authors: Y
authors: He
authors: The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China.
authors: Xin
authors: X
authors: Deng
authors: College of Pharmacy, Hebei University, Baoding 071002, China.
authors: Xiang-Hua
authors: XH
authors: Xia
authors: The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China.
authors: Jian
authors: J
authors: Liang
authors: College of Pharmacy, Hebei University, Baoding 071002, China.
authors: Geng-Liang
authors: GL
authors: Yang
authors: College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
authors: Wei
authors: 0000-0002-2988-4298
authors: W
authors: Li
authors: China-Japan Union Hospital, Jilin University, Changchun 130033, China.
authors: Hui
authors: H
authors: Wang
mesh_terms: D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D019220:High-Energy Shock Waves; D030017:Ilex / Q000737:chemistry*; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification*; D024301:Plant Bark / Q000737:chemistry*
chemical_list: D005960:Glucosides; D010666:Phenylpropionates; C028305:syringin