title: Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples.
abstract: A novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe than cervical intraepithelial neoplasia grade 3 (CIN3+), 83% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72% tested E6 positive and 92% tested high-risk (HR)-HPV DNA positive (P = 0.2). Among the women with a less than CIN3 diagnosis, 31% and 47% tested positive for E6 and HR-HPV DNA, respectively (P = 0.0006).
authors: OncoHealth Corporation, Fremont, CA, USA.
authors: Yi-Shan
authors: YS
authors: Yang
authors: Karen
authors: K
authors: Smith-McCune
authors: Teresa M
authors: TM
authors: Darragh
authors: Yvonne
authors: Y
authors: Lai
authors: Ju-Hwa
authors: JH
authors: Lin
authors: Ting-Chang
authors: TC
authors: Chang
authors: Hsiao-Yun
authors: HY
authors: Guo
authors: Tiea
authors: T
authors: Kesler
authors: Alicia
authors: A
authors: Carter
authors: Philip E
authors: PE
authors: Castle
authors: Shuling
authors: S
authors: Cheng
mesh_terms: D003584:Cytological Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000473:pathology*
chemical_list: D009856:Oncogene Proteins, Viral