title: Transepithelial transport of theasinensins through Caco-2 cell monolayers and their absorption in Sprague-Dawley rats after oral administration.
abstract: The aim of this study is to illustrate the in vivo and in vitro absorption of theasinensins B and A that are (-)-epigallocatechin-3-O-gallate (EGCG)-(-)-epigallocatechin (EGC) dimer and EGCG dimer, respectively, and their transport pathway across the intestinal membrane. Our animal study by a single oral administration to rats demonstrated the intact absorption of theasinensins into the blood system, which was estimated to be a >10-fold lower absorption amount than EGCG. The in vitro absorption study indicated that theasinensins can be transported across Caco-2 cell monolayers, while their permeability coefficients were also >10-fold lower than those of EGCG and EGC. Transport experiments using cytochalasin D or quercetin as a tight junction (TJ) modulator and a non-saturable permeation revealed that theasinensins were transported across Caco-2 cells in a TJ paracellular diffusion route. In conclusion, the dimers of condensed catechins, theasinensins B and A, can be absorbed intact into rat blood and transported across Caco-2 cell monolayers probably through a TJ paracellular pathway.
authors: Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, Hakozaki, Fukuoka, Japan.
authors: Ju
authors: J
authors: Qiu
authors: Yuko
authors: Y
authors: Kitamura
authors: Yuji
authors: Y
authors: Miyata
authors: Shizuka
authors: S
authors: Tamaru
authors: Kazunari
authors: K
authors: Tanaka
authors: Takashi
authors: T
authors: Tanaka
authors: Toshiro
authors: T
authors: Matsui
mesh_terms: D000042:Absorption; D000818:Animals; D001578:Benzopyrans / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D001692:Biological Transport; D018938:Caco-2 Cells; D002392:Catechin / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics; D002463:Cell Membrane Permeability; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D008297:Male; D010636:Phenols / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001578:Benzopyrans; D010636:Phenols; C576796:epigallocatechin-3-O-gallate epigallocatechin dimer; C481298:theasinensin A; D002392:Catechin