title: Quantitative characterization of nanoparticles in blood by transmission electron microscopy with a window-type microchip nanopipet.
abstract: Transmission electron microscopy (TEM) is a unique and powerful tool for observation of nanoparticles. However, due to the uneven spatial distribution of particles conventionally dried on copper grids, TEM is rarely employed to evaluate the spatial distribution of nanoparticles in aqueous solutions. Here, we present a microchip nanopipet with a narrow chamber width for sorting nanoparticles from blood and preventing the aggregation of the particles during the drying process, enabling quantitative analysis of their aggregation/agglomeration states and the particle concentration in aqueous solutions. This microchip is adaptable to all commercial TEM holders. Such a nanopipet proves to be a simple and convenient sampling device for TEM image-based quantitative characterization.
authors: Lin-Ai
authors: LA
authors: Tai
authors: Yu-Ting
authors: YT
authors: Kang
authors: Yu-Ching
authors: YC
authors: Chen
authors: Yu-Chao
authors: YC
authors: Wang
authors: Yu-Jing
authors: YJ
authors: Wang
authors: Yu-Ting
authors: YT
authors: Wu
authors: Kuo-Liang
authors: KL
authors: Liu
authors: Chiu-Yen
authors: CY
authors: Wang
authors: Yu-Feng
authors: YF
authors: Ko
authors: Ching-Ya
authors: CY
authors: Chen
authors: Nai-Chun
authors: NC
authors: Huang
authors: Jen-Kun
authors: JK
authors: Chen
authors: Yong-Fen
authors: YF
authors: Hsieh
authors: Tri-Rung
authors: TR
authors: Yew
authors: Chung-Shi
authors: CS
authors: Yang
mesh_terms: D006046:Gold / Q000737:chemistry; D006801:Humans; D056656:Lab-On-A-Chip Devices; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000032:analysis*; D036103:Nanotechnology / Q000295:instrumentation; D010949:Plasma / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D011092:Polyethylene Glycols; D006046:Gold