title: [Expression of reconstructed BCR-ABL-pIRES-SEA plasmids in the skeletal muscles of BALB/c mice].
abstract: This paper is aimed to investigate the transcription and expression of BCR-ABL-pIRES-SEA fusion gene vaccines in vivo in mice. The reconstructed plasmids (BCR-ABL-pIRES-SEA) which were developed previously in our laboratory were injected into the skeletal muscles of BALB/c mice at 14d intervals for three cycles. The transcription and expression of BCR-ABL and staphylococcal enterotoxin A (SEA) in injection site were detected using RT-PCR and immunohistological methods. The BCR-ABL/SEA mRNA and protein could be identified in the injection site of BCR-ABL-pIRES-SEA vaccinated mice. The reconstructed BCR-ABL-pIRES-SEA plasmids can effectively express gene production in the skeletal muscles of mice and have the common features of DNA vaccine.
authors: Department of Microbiology and Immunology, Medical College, Jinan University, Guangzhou 510632, China.
authors: Yongpeng
authors: Y
authors: Gao
authors: Yanan
authors: Y
authors: Qin
authors: Chen
authors: C
authors: Lin
authors: Hongxia
authors: H
authors: Tian
authors: Chen
authors: C
authors: Chen
authors: Yubing
authors: Y
authors: Zhou
authors: Yangqiu
authors: Y
authors: Li
mesh_terms: D000818:Animals; D004768:Enterotoxins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000378:metabolism*; D010957:Plasmids / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D004768:Enterotoxins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; C026066:enterotoxin A, Staphylococcal; D016044:Fusion Proteins, bcr-abl