title: Production of mouse pups from germline transmission-failed knockout chimeras.
abstract: Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
authors: Research Institute for Microbial Diseases, Osaka University, Yamadaoka 3-1, Suita, 565-0871, Osaka, Japan.
authors: Yoshitaka
authors: Y
authors: Fujihara
authors: Kazuhiro
authors: K
authors: Kaseda
authors: Naokazu
authors: N
authors: Inoue
authors: Masahito
authors: M
authors: Ikawa
authors: Masaru
authors: M
authors: Okabe
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002678:Chimera / Q000235:genetics / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D055786:Gene Knockout Techniques / Q000379:methods*; D005854:Germ Cells / Q000166:cytology; D049452:Green Fluorescent Proteins; D006801:Humans; D008297:Male; D051379:Mice; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D049452:Green Fluorescent Proteins