title: TLR13 recognizes bacterial 23S rRNA devoid of erythromycin resistance-forming modification.
abstract: Host protection from infection relies on the recognition of pathogens by innate pattern-recognition receptors such as Toll-like receptors (TLRs). Here, we show that the orphan receptor TLR13 in mice recognizes a conserved 23S ribosomal RNA (rRNA) sequence that is the binding site of macrolide, lincosamide, and streptogramin group (MLS) antibiotics (including erythromycin) in bacteria. Notably, 23S rRNA from clinical isolates of erythromycin-resistant Staphylococcus aureus and synthetic oligoribonucleotides carrying methylated adenosine or a guanosine mimicking a MLS resistance-causing modification failed to stimulate TLR13. Thus, our results reveal both a natural TLR13 ligand and specific mechanisms of antibiotic resistance as potent bacterial immune evasion strategy, avoiding recognition via TLR13.
authors: Institute of Medical Microbiology, University of Duisburg-Essen, Essen, Germany.
authors: Marina
authors: M
authors: Oldenburg
authors: Anne
authors: A
authors: Krüger
authors: Ruth
authors: R
authors: Ferstl
authors: Andreas
authors: A
authors: Kaufmann
authors: Gernot
authors: G
authors: Nees
authors: Anna
authors: A
authors: Sigmund
authors: Barbara
authors: B
authors: Bathke
authors: Henning
authors: H
authors: Lauterbach
authors: Mark
authors: M
authors: Suter
authors: Stefan
authors: S
authors: Dreher
authors: Uwe
authors: U
authors: Koedel
authors: Shizuo
authors: S
authors: Akira
authors: Taro
authors: T
authors: Kawai
authors: Jan
authors: J
authors: Buer
authors: Hermann
authors: H
authors: Wagner
authors: Stefan
authors: S
authors: Bauer
authors: Hubertus
authors: H
authors: Hochrein
authors: Carsten J
authors: CJ
authors: Kirschning
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000818:Animals; D024901:Drug Resistance, Multiple, Bacterial / Q000276:immunology*; D004917:Erythromycin / Q000494:pharmacology*; D006151:Guanosine / Q000378:metabolism; D055231:Lincosamides / Q000494:pharmacology; D018942:Macrolides / Q000494:pharmacology; D008745:Methylation; D051379:Mice; D012338:RNA, Ribosomal, 23S / Q000276:immunology*; D013203:Staphylococcal Infections / Q000276:immunology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000276:immunology*; D025361:Streptogramins / Q000494:pharmacology; D051193:Toll-Like Receptors / Q000276:immunology*
chemical_list: D055231:Lincosamides; D018942:Macrolides; D012338:RNA, Ribosomal, 23S; D025361:Streptogramins; C539093:Tlr13 protein, mouse; D051193:Toll-Like Receptors; D006151:Guanosine; D004917:Erythromycin; D000241:Adenosine