title: Myelinated nerve bundles developed on the plano-concave fibers containing nerve conduit.
abstract: Morphologically and chemically modified plano-concave fibers (PCFs) are designed as a unit of guided channels for supporting Schwann cells to facilitate mass transport and promote nerve regeneration. The surface-modified PCFs are imprinted with linearly patterned grooves (LPGs) to guide adherent Schwann cell elongation and axon extension. After being cocultured with PC12 neuron-like cells, Schwann cells differentiate into the myelinated type and interact with PC12 axons. The myelinated axons aggregate as a linear bundle and extend along the direction of LPGs on a PCF. The cross section of a myelin structure is examined using a transmission electron microscope. The PCFs can potentially bridge gaps in injured nerves, improving the therapeutic efficacy of nerve regeneration.
authors: Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.
authors: Wei-Chin
authors: WC
authors: Huang
authors: Jiunn-Der
authors: JD
authors: Liao
authors: Chou-Ching K
authors: CC
authors: Lin
authors: Ming-Shaung
authors: MS
authors: Ju
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D018920:Coculture Techniques; D005456:Fluorescent Dyes / Q000378:metabolism; D048091:Guided Tissue Regeneration / Q000379:methods*; D019344:Lactic Acid / Q000493:pharmacokinetics; D009413:Nerve Fibers, Myelinated / Q000187:drug effects / Q000502:physiology*; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology*; D016716:PC12 Cells; D011091:Polyesters; D011108:Polymers / Q000493:pharmacokinetics; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)