title: Performance of a rapid molecular multiplex assay for the detection of influenza and picornaviruses.
abstract: BACKGROUND
  Multiplex assays are a new strategy for diagnosing respiratory infections. These assays are better than those based on cultures or antigen detection, but few data are available for comparing them to monoplex polymerase chain reactions (PCRs). This study evaluated the performance of the Luminex xTAG Respiratory Viral Panel (RVP) Fast assay with reference to 2 real-time PCR assays for detecting type A influenza H1 viruses and human enteroviruses and rhinoviruses.
  
  
  METHODS
  This was an analysis of nasal swab specimens obtained from 590 outpatients suffering from acute respiratory tract disease between September 2009 and February 2010.
  
  
  RESULTS
  The RVP Fast assay performed well in less than 4 h for detecting type A influenza H1 viruses, particularly (H1N1)pdm09, and human entero/rhinoviruses, with 95.2% and 90.05% agreement, respectively, when compared to monoplex real-time PCR assays. This multiplex assay also detected at least 1 virus in 69.3% of the specimens and detected multiple infections in 40 samples.
  
  
  CONCLUSIONS
  The multiplex assay detected clinically important viruses in a single genomic test. It will thus be useful for detecting several viruses causing respiratory tract disorders.
authors: CHU de Toulouse, Hôpital Purpan, Laboratoire de Virologie, Toulouse, France. mansuy.jm@chu-toulouse.fr
authors: Jean-Michel
authors: JM
authors: Mansuy
authors: Catherine
authors: C
authors: Mengelle
authors: Isabelle
authors: I
authors: Da Silva
authors: Isidore
authors: I
authors: Grog
authors: Karine
authors: K
authors: Sauné
authors: Jacques
authors: J
authors: Izopet
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D009980:Influenza A virus / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000175:diagnosis* / Q000821:virology; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009297:Nasal Mucosa / Q000821:virology; D010850:Picornaviridae Infections / Q000175:diagnosis* / Q000821:virology; D012141:Respiratory Tract Infections / Q000821:virology*; D012229:Rhinovirus / Q000235:genetics / Q000302:isolation & purification*; D055815:Young Adult