title: Effects of curcumin on bleomycin‑induced oxidative stress in malignant             testicular germ cell tumors.
abstract: Bleomycin is commonly used in the treatment of testicular cancer. Bleomycin             generates oxygen radicals, induces the oxidative cleavage of DNA strands and induces             cancer cell apoptosis. Curcumin (diferuloylmethane) is a potent antioxidant and             chief component of the spice turmeric. No study investigating the effects of curcumin             on intrinsic and bleomycin-induced oxidative stress in testicular germ cell tumors             has been reported in the literature. For this reason, the present study aimed             to examine the effects of curcumin on oxidative stress produced in wild-type NTera-2             and p53-mutant NCCIT testicular cancer cells incubated with bleomycin and the             results were compared with cells treated with H2O2 which directly produces oxidative             stress. The protein carbonyl content, thiobarbituric acid reactive substances             (TBARS), glutathione (GSH), 8-isoprostane, lipid hydroperoxide (LPO) levels and             total antioxidant capacity in the two testicular cancer cell lines were determined.             Results showed that bleomycin and H2O2 significantly increased protein carbonyl,             TBARS, 8-isoprostane and LPO levels in the NTera-2 and NCCIT cell lines. Bleomycin             and H2O2 significantly decreased the antioxidant capacity and GSH levels in NTera-2             cells. Curcumin significantly decreased LPO, 8-isoprostane and protein carbonyl             content, and TBARS levels increased in cells treated with bleomycin and H2O2.             Curcumin enhanced GSH levels and the antioxidant capacity of NTera-2 cells. In             conclusion, curcumin inhibits bleomycin and H2O2-induced oxidative stress in human             testicular cancer cells.
authors: Department of Biochemistry, Faculty of Medicine, Akdeniz University,                     Antalya 07070, Turkey.
authors: Aysegul
authors: A
authors: Cort
authors: Evrim
authors: E
authors: Ozdemir
authors: Mujgan
authors: M
authors: Timur
authors: Tomris
authors: T
authors: Ozben
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology* / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009373:Neoplasms, Germ Cell and Embryonal / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects; D013736:Testicular Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D001761:Bleomycin; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D006861:Hydrogen Peroxide; D005978:Glutathione; D003474:Curcumin