title: Transplantation of nano-bioglass/gelatin scaffold in a non-autogenous setting for bone regeneration in a rabbit ulna.
abstract: Bioactive glass has been investigated for variety of tissue engineering applications. In this study, fabrication, in vitro and in vivo evaluation of bioactive glass nanocomposite scaffold were investigated. The nanocomposite scaffolds with compositions based on gelatin and bioactive glass nanoparticles were prepared. The apatite formation at the surface of the nanocomposite samples confirmed by Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray powder diffraction analyses. The in vitro characteristics of bioactive glass scaffold as well as the in vivo bone formation capacity of the bioactive glass scaffold in rabbit ulnar model were investigated. The bioactive glass scaffold showed no cytotoxicity effects in vitro. The nanocomposite scaffold made from gelatin and bioactive glass nanoparticles could be deliberated as an extremely bioactive and prospective bone tissue engineering implant. Bioactive glass scaffolds were capable of guiding bone formation in a rabbit ulnar critical-sized-defect model. Radiographic evaluation indicated that successful bridging of the critical-sized defect on the sides both next to and away from the radius took place using bioactive glass scaffolds. X-ray analysis also proposed that bioactive glass scaffolds supported normal bone formation via intramembranous formation.
authors: Department of Chemistry, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
authors: Forough
authors: F
authors: Hafezi
authors: Fatemeh
authors: F
authors: Hosseinnejad
authors: Abbas Ali Imani
authors: AA
authors: Fooladi
authors: Soroush Mohit
authors: SM
authors: Mafi
authors: Afsaneh
authors: A
authors: Amiri
authors: Mohammad Reza
authors: MR
authors: Nourani
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D004797:Enzyme-Linked Immunosorbent Assay; D005780:Gelatin; D005898:Glass; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology; D033362:Powder Diffraction; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds; D014457:Ulna / Q000502:physiology*
chemical_list: D005780:Gelatin