title: Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos.
abstract: BACKGROUND
  The 7S globulins are plant seed storage proteins that have been associated with the development of a number of human diseases, including peanut allergy. Immune reactivity to the wheat seed storage protein globulin-3 (Glo-3) has been associated with the development of the autoimmune disease type 1 diabetes in diabetes-prone rats and mice, as well as in a subset of human patients.
  
  
  RESULTS
  The present study characterized native wheat Glo-3 in salt-soluble wheat seed protein extracts. Glo-3-like peptides were observed primarily in the wheat embryo. Glo-3-like proteins varied significantly in their molecular masses and isoelectric points, as determined by two dimensional electrophoresis and immunoblotting with anti-Glo-3A antibodies. Five major polypeptide spots were identified by mass spectrometry and N-terminal sequencing as belonging to the Glo-3 family.
  
  
  CONCLUSIONS
  These results in combination with our previous findings have allowed for the development of a hypothetical model of the post-translational events contributing to the wheat 7S globulin profile in mature wheat kernels.
authors: Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Canada.
authors: Adam G
authors: AG
authors: Koziol
authors: Evelin
authors: E
authors: Loit
authors: Melissa
authors: M
authors: McNulty
authors: Amanda J
authors: AJ
authors: MacFarlane
authors: Fraser W
authors: FW
authors: Scott
authors: Illimar
authors: I
authors: Altosaar
mesh_terms: D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D056625:Endosperm / Q000378:metabolism; D005916:Globulins / Q000737:chemistry / Q000378:metabolism*; D007526:Isoelectric Point; D008954:Models, Biological; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D020539:Sequence Analysis, Protein; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000196:embryology / Q000378:metabolism*
chemical_list: D005916:Globulins; D010940:Plant Proteins; C038234:storage protein, Triticum aestivum