title: Performance of an organic photodiode as an optical detector and its application to fluorometric flow-immunoassay for IgA.
abstract: The performance of an organic thin film photodiode (OPD), fabricated from a hetero-junction comprised of two layers of C(60) and a phthalocyanine-Cu(II) complex was evaluated by detecting the chemiluminescence generated from the reaction of luminol with horseradish peroxidase in the presence of H(2)O(2), and the fluorescence from resorufin, as an optical detector. The photocurrent of the OPD was linear with respect to the power of light from a commercial LED. The sensitivity of the OPD was sufficient for detecting chemiluminescence with a power 0.1μW/cm(2). The OPD was successfully used in a flow-immunoassay for IgA, a marker of human stress, in which a sandwich immunoassay was carried out on the microchip and the fluorescence from resorufin, produced by the enzymatic reaction, was detected. The detection limits for resorufin and IgA were 5.0μM and 16ng/mL, respectively. The photosensitivity of the OPD remained relatively constant for a minimum of one year.
authors: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors: Mayo
authors: M
authors: Miyake
authors: Hizuru
authors: H
authors: Nakajima
authors: Akihide
authors: A
authors: Hemmi
authors: Masayuki
authors: M
authors: Yahiro
authors: Chihaya
authors: C
authors: Adachi
authors: Nobuaki
authors: N
authors: Soh
authors: Ryoichi
authors: R
authors: Ishimatsu
authors: Koji
authors: K
authors: Nakano
authors: Katsumi
authors: K
authors: Uchiyama
authors: Toshihiko
authors: T
authors: Imato
mesh_terms: D000818:Animals; D002417:Cattle; D004553:Electric Conductivity; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods*; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods*; D037741:Fullerenes / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007070:Immunoglobulin A / Q000032:analysis*; D007211:Indoles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques; D055620:Optical Phenomena; D009942:Organometallic Compounds / Q000737:chemistry*; D010078:Oxazines / Q000737:chemistry
chemical_list: D037741:Fullerenes; D007070:Immunoglobulin A; D007211:Indoles; D009942:Organometallic Compounds; D010078:Oxazines; C015445:copper phthalocyanine; C014180:resorufin; C069837:fullerene C60