title: Expression of podoplanin is a rare event in sporadic gastrointestinal stromal tumors and does not influence prognosis.
abstract: OBJECTIVE
  Podoplanin overexpression is associated with worse prognosis in several human cancers. In gastrointestinal stromal tumors (GISTs) very few data on the expression of podoplanin exist, but it seems to be frequently overexpressed in pediatric/syndromic GISTs. We investigated podoplanin expression and its clinical relevance in a large series of sporadic GISTs.
  
  
  METHODS
  Podoplanin expression was determined immunohistochemically in 145 sporadic adult GISTs. Aneuploidies of 1p36 and 1q25 were investigated using FISH, and KIT and PDGFRA genes were investigated by sequencing.
  
  
  RESULTS
  Overexpression of podoplanin was observed in eight (5.6%) GISTs and no association with amplification of 1p36 or KIT or PDGFRA mutations was seen. The amount of podoplanin expression was not associated with clinical risk factors or patient survival.
  
  
  CONCLUSIONS
  Overexpression of podoplanin is a rare event in sporadic GISTs and is not associated with amplification of 1p36 or with KIT or PDGFRA mutations, which indicates limited pathobiological or clinical relevance.
authors: Department of Surgery, Upper GI Research Unit, Medical University of Vienna, General Hospital, Waehringer Guertel 18-20, A-1090 Vienna, Austria. sebastian.schoppmann@meduniwien.ac.at
authors: Sebastian F
authors: SF
authors: Schoppmann
authors: Anna Sophie
authors: AS
authors: Berghoff
authors: Bettina
authors: B
authors: Jesch
authors: Johannes
authors: J
authors: Zacherl
authors: Nadine
authors: N
authors: Nirtl
authors: Gerd
authors: G
authors: Jomrich
authors: Florian
authors: F
authors: Maroske
authors: Berthold
authors: B
authors: Streubel
authors: Ildiko
authors: I
authors: Mesteri
authors: Peter
authors: P
authors: Birner
mesh_terms: D000328:Adult; D000369:Aged, 80 and over; D002878:Chromosomes, Human, Pair 1; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D046152:Gastrointestinal Stromal Tumors / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D009154:Mutation; D011379:Prognosis; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics; D012189:Retrospective Studies; D015996:Survival Rate; D000098853:Podoplanin
chemical_list: D008562:Membrane Glycoproteins; D019009:Proto-Oncogene Proteins c-kit; D020796:Receptor, Platelet-Derived Growth Factor alpha; D017479:Receptors, Platelet-Derived Growth Factor; C117468:PDPN protein, human; D000098853:Podoplanin