title: Acute exposure to DE-71: effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae.
abstract: The aim of the present study was to investigate the acute developmental neurotoxicity of polybrominated diphenyl ethers (PBDEs) in zebrafish larvae. From 2 to 120 h postfertilization zebrafish embryos were exposed to DE-71 (0, 31.0, 68.7, and 227.6 µg/L). The authors studied the locomotor behavior of larvae, involvement of the cholinergic system, and selected gene and protein expressions in the central nervous system. The results showed that low DE-71 concentration caused hyperactivity, whereas higher concentrations decreased activity during the dark period. During the light period, larval activity was significantly reduced in a concentration-dependent manner. In the cholinergic system, acetylcholinesterase activity significantly increased (10.7 and 12.4%) in the 68.7 and 227.6 µg/L exposure groups, respectively, and acetylcholine concentration accordingly decreased (60.5%) in the 227.6 µg/L exposure group. The mRNA expressions of genes encoding myelin basic protein, neuron microtubule protein (α1-tubulin), and sonic hedgehog a were significantly downregulated. Western blotting assay demonstrated that the protein concentration of α1-tubulin was also decreased. Overall, the present study demonstrated that acute exposure to PBDEs can disrupt the neurobehavior of zebrafish larvae and affect cholinergic neurotransmission and neuron development.
authors: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
authors: Lianguo
authors: L
authors: Chen
authors: Changjiang
authors: C
authors: Huang
authors: Chenyan
authors: C
authors: Hu
authors: Ke
authors: K
authors: Yu
authors: Lihua
authors: L
authors: Yang
authors: Bingsheng
authors: B
authors: Zhou
mesh_terms: D000109:Acetylcholine / Q000032:analysis; D000110:Acetylcholinesterase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002490:Central Nervous System / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D053823:Hedgehog Proteins / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D009043:Motor Activity / Q000187:drug effects*; D004676:Myelin Basic Protein / Q000378:metabolism; D020258:Neurotoxicity Syndromes / Q000503:physiopathology*; D009435:Synaptic Transmission / Q000187:drug effects; D023382:Toxicity Tests, Acute; D014404:Tubulin / Q000378:metabolism; D015027:Zebrafish / Q000254:growth & development / Q000502:physiology*; D029961:Zebrafish Proteins / Q000378:metabolism
chemical_list: D055768:Halogenated Diphenyl Ethers; D053823:Hedgehog Proteins; D004676:Myelin Basic Protein; C524053:Shha protein, zebrafish; D014404:Tubulin; D029961:Zebrafish Proteins; C086401:pentabromodiphenyl ether; D000110:Acetylcholinesterase; D000109:Acetylcholine