title: [Role of K(Ca)3.1 channel in proliferation and migration of rat vascular smooth muscle cells of the proliferative phenotype].
abstract: OBJECTIVE
  To investigate the role of K(Ca)3.1 channel in the proliferation and migration of rat vascular smooth muscle cells of the proliferative phenotype.
  
  
  METHODS
  Rat vascular smooth muscle cells (VSMCs) were cultured with tissue adhesion method. The morphological characteristics of the fist and ninth passages of VSMCs were observed with light and electron microscopy and immunocytochemistry. The expressions of K(Ca)3.1 channel mRNA and protein in the cells were detected using RT-PCR and immunocytochemistry, respectively. MTT and transwell assay were employed to assess the effect of the K(Ca)3.1 channel blocker TRAM-34 on the proliferation and migration of VSMCs.
  
  
  RESULTS
  The first and ninth passages of VSMCs showed morphological characteristics of contractile and proliferative phenotypes, respectively. Compared with the first- passage cells, the ninth-passage VSMCs exhibited significantly increased K(Ca)3.1 channel mRNA and protein expressions with enhanced cell proliferation and migration (P<0.01), which was inhibited by the application of TRAM-34 (P<0.01). TRAM-34 produced no obvious effect on the first-passage VSMCs.
  
  
  CONCLUSIONS
  Upregulated expression of K(Ca)3.1 channel can promote the proliferation and migration of rat VSMCs of the proliferative phenotype.
authors: Department of Basic Medical Science, Xi'an Medical University, China. suxingli@126.com
authors: Xingli
authors: X
authors: Su
authors: Hong
authors: H
authors: Zhang
authors: Wei
authors: W
authors: Yu
authors: Jian
authors: J
authors: Huo
authors: Yufang
authors: Y
authors: Guo
authors: Shuang
authors: S
authors: Wang
authors: Xiang
authors: X
authors: Wang
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D051036:Large-Conductance Calcium-Activated Potassium Channels / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D026902:Potassium Channel Blockers / Q000494:pharmacology; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051036:Large-Conductance Calcium-Activated Potassium Channels; D026902:Potassium Channel Blockers; D011720:Pyrazoles; C411671:TRAM 34