title: Investigation of effects and mechanisms of total flavonoids of Astragalus and calycosin on human erythroleukemia cells.
abstract: Flavonoids are found in most parts of plants and have been shown to have multiple biological activities such as anticancer, anti-inflammation, antibacteria, antivirus, and immune-stimulation. Existing data showed that the total flavonoids of Astragalus (TFA) can provide biological system with resistance to injury and can possess antimutagenic, atherosclerotic inhibition, and other biological effects. This study investigated the effects of TFA and calycosin (a compound isolated from TFA), on apoptosis induction, and cell cycle of human erythroleukemia cell line K562 by an array of techniques, including proliferation (MTT), PI staining, Annexin V/PI double staining, and RT-PCR. The experimental data showed that TFA and calycosin could inhibit the proliferation of K562 cells. The 50% inhibiting concentrations of TFA and calycosin were 98.63 μg/mL and 130.32 μg/mL, respectively. However, TFA and calycosin could not induce apoptosis in K562 cells, but could increase the number of the cells in the G(0)/G(1) phase. The level of cyclin D1 mRNA in K562 cells decreased after the treatment with TFA and calycosin. This study provides new insights into the functional mechanism of total flavonoids of Astragalus and calycosin on human erythroleukemia cells.
authors: Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853, China.
authors: Dongqing
authors: D
authors: Zhang
authors: Yuan
authors: Y
authors: Zhuang
authors: Jichun
authors: J
authors: Pan
authors: Haibao
authors: H
authors: Wang
authors: Hui
authors: H
authors: Li
authors: Yang
authors: Y
authors: Yu
authors: Deqing
authors: D
authors: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D027883:Astragalus Plant / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology*; D020014:K562 Cells; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D005419:Flavonoids; D007529:Isoflavones; D012333:RNA, Messenger; C121707:7,3'-dihydroxy-4'-methoxyisoflavone; D019938:Cyclin D1