title: Lack of association between the rs2294008 polymorphism in the prostate stem cell antigen gene and colorectal neoplasia: a case-control and immunohistochemical study.
abstract: BACKGROUND
  Prostate stem cell antigen (PSCA) has been implicated in the pathogenesis of several solid tumours, either due to changes in protein expression, or through association with the rs2294008 polymorphism in the PSCA gene. To our knowledge, the role of PSCA in the development of colorectal neoplasia has not been explored. We performed a genotyping study to assess for associations between the rs2294008 polymorphism and risk of adenomatous polyps and colorectal cancer. DNA samples were available from 388 individuals with colorectal neoplasia and 496 controls, all of whom had undergone screening colonoscopy. In addition, we performed immunohistochemical staining for PSCA in colonic tissue representing all stages of the adenoma-carcinoma sequence.
  
  
  RESULTS
  No genotypic associations were found between the rs2294008 polymorphism and the risk of colorectal adenomata or cancer. Immunohistochemical staining did not reveal any alteration in PSCA expression accompanying the adenoma-carcinoma sequence.
  
  
  CONCLUSIONS
  From these data it seems unlikely that PSCA has a role in the initiation or progression of colorectal neoplasia.
authors: Gastrointestinal Research Group, Institute of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, Scotland.
authors: Christopher
authors: C
authors: Smith
authors: Paul
authors: P
authors: Lochhead
authors: Umesh
authors: U
authors: Basavaraju
authors: Georgina L
authors: GL
authors: Hold
authors: Nicky
authors: N
authors: Fyfe
authors: Graeme I
authors: GI
authors: Murray
authors: Emad M
authors: EM
authors: El-Omar
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D058851:GPI-Linked Proteins / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D000951:Antigens, Neoplasm; D058851:GPI-Linked Proteins; D009363:Neoplasm Proteins; C110843:PSCA protein, human