title: Lipopolysaccharide induces multinuclear cell from RAW264.7 line with increased phagocytosis activity.
abstract: Lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria, induces strong proinflammatory responses, including the release of cytokines and nitric oxide from macrophage. In this study, we found that a murine macrophage-derived line, RAW264.7, became multinuclear through cell-cell fusion after incubation with highly purified LPS or synthetic lipid A in the presence of Ca(2+). The same cell line is known to differentiate into multinuclear osteoclast, which expresses a specific proton pumping ATPase together with osteoclast markers on stimulation by the extracellular domain of receptor activator of nuclear factor κB ligand (Toyomura, T., Murata, Y., Yamamoto, A., Oka, T., Sun-Wada, G.-H., Wada, Y. and Futai, M., 2003). The LPS-induced multinuclear cells did not express osteoclast-specific enzymes including tartrate-resistant acid phosphatase and cathepsin K. During multinuclear cell formation, the cells internalized more and larger polystyrene beads (diameter 6-15 μm) than mononuclear cells and osteoclasts. The internalized beads were located in lysosome-marker positive organelles, which were probably phagolysosomes. The LPS-induced multinuclear cell could be a good model system to study phagocytosis of large foreign bodies.
authors: Department of Biochemistry, Faculty of Pharmaceutical Sciences, Iwate Medical University, Futai Special Laboratory, Yahaba, Iwate 028-3694, Japan. nakanim@iwate-med.ac.jp
authors: Mayumi
authors: M
authors: Nakanishi-Matsui
authors: Shio
authors: S
authors: Yano
authors: Naomi
authors: N
authors: Matsumoto
authors: Masamitsu
authors: M
authors: Futai
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D008050:Lipid A / Q000276:immunology / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008863:Microspheres; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D010588:Phagosomes / Q000276:immunology*; D011137:Polystyrenes / Q000276:immunology; D012475:Salmonella / Q000276:immunology
chemical_list: D008050:Lipid A; D008070:Lipopolysaccharides; D011137:Polystyrenes; D002118:Calcium