title: Evaluation of serological cross-reactivity between canine visceral leishmaniasis and natural infection by Trypanosoma caninum.
abstract: In order to evaluate if the presence of Trypanosoma caninum can lead to a confuse diagnosis of canine visceral leishmaniasis (CVL), we investigated the serological status of dogs infected by T. caninum and assessed the serological cross-reactivity with CVL. A set of 117 serum samples from dogs infected by T. caninum, Leishmania chagasi and not infected dogs (n=39 in each group) was tested using commercial kits--indirect immunofluorescence (IFI-LVC), ELISA (EIE-LVC) and immunochromatographic test (DPP)--and in house tests with T. caninum (IIF-Tc and ELISA-Tc) and L. chagasi antigens (IIF-Lc and ELISA-Lc). IIF-Tc and ELISA-Tc presented sensitivity of 64.1% and 94.9% and specificity of 23.1% and 35.9%, respectively. The sensitivity of the IFI-LVC, EIE-LVC and DPP tests was 100% and the specificity was 70.5%, 68% and 97.5% respectively. The concordance between the tests was considered as satisfactory. The specificities of IFI-LVC, EIE-LVC and DPP were higher when the group Tc was excluded, with significant values for IFI-LVC (χ2=4.36, P-value=0.036), thus suggesting that the infection by T. caninum can confuse the diagnosis of CVL.
authors: Programa de pós-graduação em Pesquisa Clínica em Doenças Infecciosas, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil 4365, 21040-900 Rio de Janeiro, RJ, Brazil.
authors: A S
authors: AS
authors: Alves
authors: E
authors: E
authors: Mouta-Confort
authors: F B
authors: FB
authors: Figueiredo
authors: R V C
authors: RV
authors: Oliveira
authors: A O
authors: AO
authors: Schubach
authors: M F
authors: MF
authors: Madeira
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D003429:Cross Reactions / Q000276:immunology; D004283:Dog Diseases / Q000175:diagnosis / Q000469:parasitology*; D004285:Dogs; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D012698:Serologic Tests / Q000662:veterinary*; D014345:Trypanosoma / Q000145:classification* / Q000276:immunology; D014352:Trypanosomiasis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D000913:Antibodies, Protozoan