title: Total glucosides of paeony attenuated functional maturation of dendritic cells via blocking TLR4/5 signaling in vivo.
abstract: It is well known that dendritic cells (DCs) play a critical role in the initiation and development of an immune response. Inhibitory effect on DC maturation alters immune-mediated inflammatory reaction in vivo. Total glucosides of paeony (TGP) are active compounds extracted from the roots of Paeonia lactiflora and have been widely used to ameliorate inflammation in therapy for autoimmune diseases. However, whether TGP act on DC maturation remains unknown. In this study, we investigated the effect of TGP on DC maturation in ovalbumin (OVA) immunized mice. Ear inflammation was inhibited by TGP (150 mgkg(-1), i.p.×11 days) obviously. The antigen presenting capacity of DC derived from TGP-treated mice was arrested. Meanwhile, OVA specific T cell proliferation was inhibited. In addition, we found that maturation of DCs was decreased by TGP treatment. Furthermore, OVA specific T cell proliferation was rescued by the adoptive transfer of mature DCs (mDCs) into TGP treated OVA-challenged mice. The research on the mechanism showed that TGP significantly inhibited activation of TLR4/5 singling. All these results demonstrated that TGP inhibited DC maturation and function by selectively blocking TLR4/5 activation in vivo, which in turn leads to reduce immune-mediated inflammation in vivo, adding a novel mechanism and therapeutic target of TGP for inflammatory and autoimmune disease treatment.
authors: Shanghai Institute of Immunology, Institute of medical sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors: Zhou
authors: Z
authors: Zhou
authors: Jinpiao
authors: J
authors: Lin
authors: Rongfen
authors: R
authors: Huo
authors: Wenkang
authors: W
authors: Huang
authors: Jian
authors: J
authors: Zhang
authors: Li
authors: L
authors: Wang
authors: Yue
authors: Y
authors: Sun
authors: Baihua
authors: B
authors: Shen
authors: Ningli
authors: N
authors: Li
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000941:Antigens / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects*; D005260:Female; D005960:Glucosides / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D029596:Paeonia; D010936:Plant Extracts / Q000494:pharmacology; D018517:Plant Roots; D013154:Spleen / Q000166:cytology; D051197:Toll-Like Receptor 4 / Q000276:immunology; D051198:Toll-Like Receptor 5 / Q000276:immunology
chemical_list: D000941:Antigens; D016207:Cytokines; D005960:Glucosides; D010936:Plant Extracts; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D051198:Toll-Like Receptor 5; D010047:Ovalbumin