title: Culture systems: embryo co-culture.
abstract: During the 1970s, domestic animal biotechnology, i.e., embryo transfer in farm animals, was confronted with the problem of embryonic developmental arrest observed in vitro, especially during the cycle in which maternal to zygotic transition (MZT) cycle takes place. In farm animals, obtaining blastocysts is mandatory, as transfer at earlier stages results in expulsion of the embryo from the vagina. In humans, the first attempts to obtain blastocysts with classical culture media were disappointing, and the use of a coculture strategy was naturally tempting: the first significant results of successful blastocyst development were obtained in the early 1980s, using trophoblastic tissue as a feeder layer in order to mimic an autocrine embryotrophic system. The next supporting cell systems were based on oviduct epithelial cells and uterine cells in order to achieve a paracrine effect. Non-hormone dependence was then demonstrated with the use of prepubertal cells, and finally with the use of established cell lines of nongenital origin (African Green Monkey Kidney, Vero cells). The embryotrophic properties are linked to features of "transport epithelia." Vero cells have been extensively used in human ART, and most of our knowledge about the human blastocyst was gathered with the use of this technology. Coculture is still in current use, but with systems that employ autologous uterine cells. Results following the use of this technology in human ART are superior to those observed with the use of sequential media. The benefit is linked to the release of free radical scavengers and growth factors by the feeder cells. In animal biotechnology, an important part of the "precious embryos," i.e., those resulting from cloning technology, involves coculture with buffalo rat liver (BRL) cells or Vero cells.
authors: UNILABS France, Paris, France. ymenezo@unilabs.fr
authors: Yves J R
authors: YJ
authors: Ménézo
authors: Edouard
authors: E
authors: Servy
authors: Anna
authors: A
authors: Veiga
authors: André
authors: A
authors: Hazout
authors: Kay
authors: K
authors: Elder
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002522:Chlorocebus aethiops; D015925:Cryopreservation; D046149:Embryo Culture Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D014599:Uterus / Q000166:cytology; D014709:Vero Cells