title: Cluster analysis of mRNA expression levels identifies multiple sequential patterns following focal cerebral ischemia.
abstract: OBJECTIVE
  The purpose of this study is to detect gene expression patterns following     focal cerebral ischemia.
  
  
  METHODS
  25 male Wistar rats were divided     into control (n = 8) and ischemic (n = 17) groups. In the ischemic group, slowly     progressing focal ischemia was simulated by two-vein occlusion with spreading     depression (SD) a cortical microinjection of KCl induced. Ischemic tissue was     removed at 2, 8, 24, or 72 h postischemia. Using semiquantitative reverse     transcription polymerase chain reaction, we investigated mRNA expression levels     of 13 representative genes related to cerebral ischemia. Cluster analysis of the     gene expression levels was done.
  
  
  RESULTS
  In the ischemic group, the expression     levels of c-fos, cyclin D1, and COX-2 were significantly higher at 2 h     postischemia, and those of bcl-2, bcl-xL, and HO-1 at 72 h. Based on the cluster     analysis, we statistically divided examined genes into three groups: group A,     early expression (COX-2, c-fos, and bcl-2); group B, s expression (c-jun, SOD-1,     bad, p53, SOD-2, bcl-xL, and bax); and group C, late expression (cyclin D1,     c-myc, and HO-1).
  
  
  CONCLUSIONS
  We statistically classified the genes into three     groups after focal ischemia. The genes of the early- and late-expression groups     can be possible therapeutic targets for the treatment of cerebral ischemia.
authors: Johannes Gutenberg University Mainz, Institute for Neurosurgical Pathophysiology, Mainz, Germany. kaido@ncnp.go.jp
authors: Takanobu
authors: T
authors: Kaido
authors: Oliver
authors: O
authors: Kempski
authors: Axel
authors: A
authors: Heimann
authors: Cara
authors: C
authors: Heers
authors: Daniela
authors: D
authors: Bartsch
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000098:blood supply; D002550:Cerebral Veins / Q000502:physiology; D016000:Cluster Analysis; D013181:Cortical Spreading Depression; D017097:Electric Impedance; D020767:Intracranial Thrombosis / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D010777:Photochemistry; D011189:Potassium Chloride / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D014158:Transcription, Genetic
chemical_list: D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011189:Potassium Chloride