title: Simultaneous determination of three sesquiterpene lactones from Herba Inula extract in rat plasma by LC/MS/MS and its application to pharmacokinetic study.
abstract: A rapid and sensitive liquid chromatography-tandem mass spectrometry method has been developed and validated for the determination of 1-acetoxy-6α-hydroxyeriolanolide, 1β-hydroxyalantolactone and ivangustin from Herba Inula extract in rat plasma. Plasma samples were pretreated by protein precipitation with methanol. Chromatographic separation was accomplished on a TOSOH TSKgel ODS column with mobile phase consisting of methanol and 0.3% formic acid (80:20, v/v). The detection was carried out by multiple-reaction monitoring mode under positive electrospray ionization. The quantification was performed using the transitions of m/z 309.1/185.0 for 1-acetoxy-6α-hydroxyeriolanolide, m/z 249.0/231.1 for 1β-hydroxyalantolactone and ivangustin and m/z 285.0/193.0 for diazepam, respectively. Calibration curves were linear over the concentration range of 4-800 ng/mL for 1-acetoxy-6α-hydroxyeriolanolide, 8-500 ng/mL for 1β-hydroxyalantolactone and ivangustin. The limit of detection (LOD) was 1 ng/mL for 1-acetoxy-6α-hydroxyeriolanolide, 1.6 ng/mL for 1β-hydroxyalantolactone and ivangustin (S/N=3). The intra-day and inter-day precisions (RSD%) for the three compounds were less than 7.8% and 8.6%, and the accuracy (RE%) ranged from -4.6 to 6.8%. The method was successfully applied to pharmacokinetic studies of the three sesquiterpene lactones after oral administration of 300 mg/kg Herba Inula extract to rats, the t(½) of 1-acetoxy-6α-hydroxyeriolanolide, 1β-hydroxyalantolactone and ivangustin was 9.65±1.43, 14.88±0.82 and 13.93±2.74 (h). The AUC((0-t)) of 1-acetoxy-6α-hydroxyeriolanolide, 1β-hydroxyalantolactone and ivangustin was 1102.46±247.04, 808.92±117.53 and 990.35±275.49 (ng h/mL), respectively.
authors: Department of Phytochemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China.
authors: Xi
authors: X
authors: Yang
authors: Juan
authors: J
authors: Su
authors: Yajun
authors: Y
authors: He
authors: Hui
authors: H
authors: Liu
authors: Haiyun
authors: H
authors: Li
authors: Weidong
authors: W
authors: Zhang
mesh_terms: D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D038143:Inula / Q000737:chemistry*; D057230:Limit of Detection; D008297:Male; D010936:Plant Extracts / Q000097:blood / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000097:blood* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010936:Plant Extracts; D012717:Sesquiterpenes