title: Veillonella tobetsuensis sp. nov., an anaerobic, gram-negative coccus isolated from human tongue biofilms.
abstract: Four previously unknown, gram-negative, anaerobic coccal strains were isolated from the tongue biofilm of healthy human adults (ages 22-29 years). The isolates displayed all phenotypic characteristics of the genus Veillonella. Comparative analysis of the 16S rRNA, dnaK and rpoB gene sequences indicated that the four strains were phylogenetically homogeneous and comprised a distinct novel lineage within the genus Veillonella. The production of major cellular fatty acids (C13 : 0 and C17 : 1ω8) was consistent with that of other members of the genus Veillonella. Based on these observations, strains B16(T), A16, B4 and Y6 represent a novel species, for which the name Veillonella tobetsuensis sp. nov. is proposed, with the type strain B16(T) ( = JCM 17976(T) = ATCC BAA-2400(T)).
authors: Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
authors: Izumi
authors: I
authors: Mashima
authors: Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
authors: Arihide
authors: A
authors: Kamaguchi
authors: Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
authors: Hiroshi
authors: H
authors: Miyakawa
authors: Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
authors: Futoshi
authors: F
authors: Nakazawa
mesh_terms: D000328:Adult; D015373:Bacterial Typing Techniques; D018441:Biofilms; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014059:Tongue / Q000382:microbiology*; D014678:Veillonella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S