title: Identification and quantification of 1-hydroxybutene-2-yl mercapturic acid in human urine by UPLC- HILIC-MS/MS as a novel biomarker for 1,3-butadiene exposure.
abstract: 1,3-Butadiene (BD) is a Class 1 carcinogen present at workplaces, in polluted air, in automobile exhaust, and in tobacco smoke. 2-Hydroxybutene-1-yl mercapturic acid (2-MHBMA) is a urinary metabolite often measured as a biomarker for exposure to BD. Here, we show for the first time that an additional MHBMA isomer is present at significant amounts in human urine, 1-hydroxybutene-2-yl mercapturic acid (1-MHBMA). For its quantification, a highly sensitive UPLC-HILIC-MS/MS method was developed and validated. Analyzing urinary samples of 183 volunteers, we demonstrate that 1-MHBMA is a novel and potentially more reliable biomarker for BD exposure than the commonly analyzed 2-MHBMA.
authors: ABF Analytisch-Biologisches Forschungslabor GmbH, 80336 Munich, Germany.
authors: Katharina
authors: K
authors: Sterz
authors: Gerhard
authors: G
authors: Scherer
authors: Jan
authors: J
authors: Krumsiek
authors: Fabian J
authors: FJ
authors: Theis
authors: Josef
authors: J
authors: Ecker
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000652:urine; D000393:Air Pollutants / Q000737:chemistry / Q000378:metabolism*; D015415:Biomarkers / Q000652:urine; D002070:Butadienes / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007536:Isomerism; D016273:Occupational Exposure; D053719:Tandem Mass Spectrometry
chemical_list: C576990:1-hydroxybutene-2-yl mercapturic acid; D000393:Air Pollutants; D015415:Biomarkers; D002070:Butadienes; C031763:1,3-butadiene; D000111:Acetylcysteine