title: Combined immunization of mice with DNA, rMVA and rAd5 expressing HIV-1 structural genes from different subtypes.
abstract: The objective of present paper is to study the immunogenicity of combinations of multiple vector vaccines expressing HIV-1 structural genes from different subtypes. Mice were vaccinated with DNA (B'/C) and rMVA (B'/C) vaccines expressing B'/C recombinant subtype gag-pol and env genes, DNA (B') and rAd5 (B') vaccines expressing subtype B' gag gene with different combination schemes. HIV-1 Gag-specific cellular immune responses and P24- specific IgG levels were analyzed by IFN-γ enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA) respectively. ELISPOT results indicated that the Gag-specific cellular immune responses induced by combination of three vaccines were much higher than that induced by combination of two vaccines. Among the groups of mice immunized with two vaccines, the groups with rAd5 booster elicited higher cellular immune responses compared with the groups with rMVA booster. All the test groups of three vaccines in combination could induce similar level of cellular immune responses, which did not correlate with the immunization order. ELISA results showed that p24- specific IgG induced by combination of three vaccines were much higher than that induced by combination of two vaccines. It indicates that the combination scheme of multiple vector vaccines maybe a promising AIDS vaccine strategy.
authors: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
authors: Lingfei
authors: L
authors: Zhang
authors: Liu
authors: L
authors: Yang
authors: Xia
authors: X
authors: Feng
authors: Zhulun
authors: Z
authors: Zhuang
authors: Shuangqing
authors: S
authors: Yu
authors: Ling
authors: L
authors: Yang
authors: Hongxia
authors: H
authors: Li
authors: Xianghui
authors: X
authors: Yu
authors: Wei
authors: W
authors: Kong
authors: Yi
authors: Y
authors: Zeng
mesh_terms: D016915:AIDS Vaccines / Q000494:pharmacology*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation; D015683:Gene Products, gag / Q000235:genetics; D015684:Gene Products, pol / Q000235:genetics; D015483:HIV Antibodies / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular / Q000235:genetics; D007114:Immunization; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019444:Vaccines, DNA / Q000494:pharmacology; D019562:Viral Load; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*
chemical_list: D016915:AIDS Vaccines; D015683:Gene Products, gag; D015684:Gene Products, pol; D015483:HIV Antibodies; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D054299:env Gene Products, Human Immunodeficiency Virus; C097729:gp140 envelope protein, Human immunodeficiency virus 1