title: Alpha 1-antitrypsin: a novel tumor-associated antigen identified in patients with early-stage breast cancer.
abstract: Several studies have demonstrated that sera from patients with cancer contain antibodies that recognize a unique group of autologous antigens called tumor-associated antigens (TAA). In the current study, we employed an immunoproteomic approach, combining 2DE, Western blot, and MALDI-MS to identify TAA in the sera of patients diagnosed with infiltrating ductal or in situ carcinoma breast cancer. Sera obtained from 25 newly diagnosed patients with stage II breast cancer and 20 healthy volunteers was evaluated for the presence of novel TAA. Alpha 1-antitrypsin (A1AT) antibodies were detected in 24 of 25 patients with breast cancer (96%) and in 2 of 20 controls (10%). Sensitivity of detection of autoantibodies against A1AT in patients with breast cancer was 96%. Our preliminary results suggest that A1AT and autoantibodies against alpha 1 antitrypsin may be useful serum biomarkers for early-stage breast cancer screening and diagnosis.
authors: Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C.-CIATEJ, Guadalajara, México.
authors: Eneida
authors: E
authors: López-Árias
authors: Adriana
authors: A
authors: Aguilar-Lemarroy
authors: Luis
authors: L
authors: Felipe Jave-Suárez
authors: Gilberto
authors: G
authors: Morgan-Villela
authors: Ignacio
authors: I
authors: Mariscal-Ramírez
authors: Moisés
authors: M
authors: Martínez-Velázquez
authors: Angel H
authors: AH
authors: Alvarez
authors: Abel
authors: A
authors: Gutiérrez-Ortega
authors: Rodolfo
authors: R
authors: Hernández-Gutiérrez
mesh_terms: D000328:Adult; D000906:Antibodies / Q000097:blood / Q000276:immunology; D000951:Antigens, Neoplasm / Q000097:blood* / Q000276:immunology; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D002278:Carcinoma in Situ / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D018270:Carcinoma, Ductal, Breast / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008875:Middle Aged; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000515:alpha 1-Antitrypsin / Q000097:blood* / Q000276:immunology
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D000515:alpha 1-Antitrypsin