title: Antimicrobial resistance and molecular typing of pseudomonas aeruginosa isolated from surgical wounds in Lagos, Nigeria.
abstract: The aim of the study was to determine the resistance patterns of Pseudomonas aeruginosa isolates recovered from patients with surgical wounds in hospitals and also to investigate their epidemiological relatedness using molecular typing techniques. Twenty Pseudomonas sp. isolated from surgical wounds were subjected to antibiotic susceptibility testing by disk diffusion, plasmid profile, SDS-PAGE and PCR using the parC, gyr A gene and RAPD using the 1254 primer. The isolates showed resistance to 12 different antibiotics with six being 100% resistant. Plasmids were detected in 16 (80%) of the isolates. The RAPD-PCR using the primer 1254, SDS-PAGE classified the 20 Pseudomonas spp. into 5 and 6 types respectively. Pseudomona aeruginosa strains isolated from surgical wounds were generally resistant to a broad range of antibiotics and this is rather worrisome. The typing techniques classified the 20 isolates into 5 and 6 groups.
authors: Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, 6 Edmond Crescent, Yaba, Lagos, Nigeria. stellaismith@yahoo.com
authors: Stella
authors: S
authors: Smith
authors: Olaniyi
authors: O
authors: Ganiyu
authors: Rachael
authors: R
authors: John
authors: Muinah
authors: M
authors: Fowora
authors: Kehinde
authors: K
authors: Akinsinde
authors: Peter
authors: P
authors: Odeigah
mesh_terms: D006801:Humans; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D009549:Nigeria; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification; D013530:Surgical Wound Infection / Q000382:microbiology*