title: Oral delivery of near-infrared quantum dot loaded micelles for noninvasive biomedical imaging.
abstract: The purpose of this study is to design, develop, and characterize an optical imaging agent for oral administration. The hydrophobic, nanosized (7 nm), near-infrared (NIR) quantum dots (QDs) have been loaded into deoxycholic acid (DOCA) conjugated low molecular weight heparin (LMWH) micelles. The QD-loaded LMWH-DOCA (Q-LHD) nanoparticles have been characterized by electrophoretic light scattering (ELS) and a transmission electron microscope (TEM) which shows the average particle size was 130-220 nm in diameter. The Q-LHD nanoparticles also show the excellent stability in different pH conditions, and the release profile demonstrates the slow release of QDs after 5 days of oral administration. Concfocal laser microscopic scanning images show that the Q-LHD nanoparticles penetrate the cell membrane and are located inside the cell membrane. The real time pharmacokinetics studies show the absorption, distribution, metabolism, and elimination profile of Q-LHD nanoparticles, observed by the Kodak molecular imaging system (KMIS). This study has demonstrated that the orally administered Q-LHD nanoparticles are absorbed in the small intestine through the bile acid transporter and eliminated through the kidneys.
authors: Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungbuk 380-702, Republic of Korea.
authors: Zehedina
authors: Z
authors: Khatun
authors: Md
authors: M
authors: Nurunnabi
authors: Kwang Jae
authors: KJ
authors: Cho
authors: Yong-Kyu
authors: YK
authors: Lee
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001671:Biochemistry / Q000379:methods; D018938:Caco-2 Cells; D002104:Cadmium / Q000737:chemistry; D003840:Deoxycholic Acid / Q000737:chemistry; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light; D051379:Mice; D008819:Mice, Nude; D008823:Micelles; D046529:Microscopy, Electron, Transmission / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry; D045663:Quantum Dots; D012542:Scattering, Radiation; D019265:Spectroscopy, Near-Infrared / Q000379:methods
chemical_list: D006495:Heparin, Low-Molecular-Weight; D008823:Micelles; D003840:Deoxycholic Acid; D002104:Cadmium