title: 15α-Hydroxylation of a steroid (13-ethyl-gon-4-en-3,17-dione) by Penicillium raistrickii in an ionic liquid/aqueous biphasic system.
abstract: Biphasic processes are used in whole-cell biotransformation to overcome the low water solubility of substrates and products as well as their inhibitory effects on the biocatalyst. Commercially available [NTf(2)]- and [PF(6)]-based ionic liquids (ILs) were used in a biphasic system for the 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione by Penicillium raistrickii. With the substrate at 5 g l(-1) and a volume ratio of IL to buffer, buffer pH and cell density at, 1:9, 6.5, 16.8 g(DW) l(-1), respectively, the 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione was achieved with a yield of 70 % after 72 h using [BMIm][NTf(2)] in a 50 ml biphasic system. This is compared to a 30 % yield in a monophasic aqueous system. This suggests the potential industrial application of ILs-based biphasic systems for steroid biotransformation.
authors: Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science & Technology, Tianjin, People's Republic of China. shuhongmao@126.com
authors: Shuhong
authors: S
authors: Mao
authors: Xiaojie
authors: X
authors: Hu
authors: Boyuan
authors: B
authors: Hua
authors: Na
authors: N
authors: Wang
authors: Xiaoguang
authors: X
authors: Liu
authors: Fuping
authors: F
authors: Lu
mesh_terms: D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D001711:Biotransformation / Q000502:physiology*; D006067:Gonanes / Q000737:chemistry / Q000378:metabolism*; D006900:Hydroxylation; D007093:Imidazoles / Q000737:chemistry; D052578:Ionic Liquids / Q000737:chemistry*; D010407:Penicillium / Q000378:metabolism*; D014867:Water
chemical_list: D006067:Gonanes; D007093:Imidazoles; D052578:Ionic Liquids; D014867:Water; C055308:13-ethyl-gon-4-ene-3,17-dione