title: Regulatable vectors for environmental gene expression in Alphaproteobacteria.
abstract: Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.
authors: Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Norwich, United Kingdom.
authors: Adrian J
authors: AJ
authors: Tett
authors: Steven J
authors: SJ
authors: Rudder
authors: Alexandre
authors: A
authors: Bourdès
authors: Ramakrishnan
authors: R
authors: Karunakaran
authors: Philip S
authors: PS
authors: Poole
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004783:Environmental Microbiology; D018628:Gene Dosage; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D008969:Molecular Sequence Data; D010944:Plants / Q000382:microbiology; D010957:Plasmids; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial