title: Study of the relationship between human MIF level, MIF-794CATT5-8 microsatellite polymorphism, and susceptibility of tuberculosis in Southwest China.
abstract: OBJECTIVE
  To study the human migration inhibitory factor (MIF) level in tuberculosis (TB) patients, and the relationship between MIF-794CATT microsatellite polymorphism and susceptibility of TB in Southwest China.
  
  
  METHODS
  TB patients (n=151) and healthy unrelated controls (n=149) were recruited for this study. Genomic DNA was extracted, and then amplified by polymerase chain reaction (PCR). MIF-794CATT(5-8) microsatellite polymorphism was genotyped by DNA sequencing. MIF level was detected by ELISA.
  
  
  RESULTS
  In the TB group, the repeat number of 7/7 and 7/8 (17.89%) was significantly higher than that of the control group (8.05%), and the serum MIF level was also much higher than that of the healthy controls (705.21 ± 67.98 vs. 355.31 ± 57.29 pg/mL, p<0.01).
  
  
  CONCLUSIONS
  The appearance of MIF-794CATT 7/7 and 7/8 is associated with susceptibility to TB, and may play an important role in the occurrence and development of TB in Southwest China.
authors: Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, China.
authors: Yanlin
authors: Y
authors: Li
authors: Zhaofang
authors: Z
authors: Zeng
authors: Shaoli
authors: S
authors: Deng
mesh_terms: D000328:Adult; D001483:Base Sequence; D016022:Case-Control Studies; D002681:China; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008263:Macrophage Migration-Inhibitory Factors / Q000097:blood* / Q000235:genetics; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D014376:Tuberculosis / Q000235:genetics*
chemical_list: D008263:Macrophage Migration-Inhibitory Factors