title: Electrochemical immunosensor for label free epidermal growth factor receptor (EGFR) detection.
abstract: This paper presents an electrochemical immune sensor for label free detection of epidermal growth factor receptor (EGFR) by immobilizing anti-EGFR antibody (Anti-EGFRab) on dithiobissuccinimidyl propionate (DTSP) self-assembled monolayer (SAM) on gold (Au) electrode. Electrochemical studies show that increased surface concentration of redox moieties onto Anti-EGFRab/DTSP immuno-electrode leads to high electron transport and improved sensing performance. The antigen-antibody complex demonstrates a high association constant (5×10(12)L/mol) that results in high affinity of Anti-EGFRab to EGFR, confirming that the DTSP-SAM provides a conducive environment for anti-EGFR immobilization. The electrochemical response of EA/Anti-EGFRab/DTSP/Au electrode as a function of EGFR concentrations exhibits a linear range from 1pg/mL to 100ng/mL, a detection limit of 1pg/mL at a sensitivity of 2.02μA M(-1)at a regression coefficient of 0.99.
authors: Bio-MEMS and Microsystems Lab, Department of Biomedical Engineering, Florida International University, Miami, FL 33174, USA. avasu002@fiu.edu
authors: Abhay
authors: A
authors: Vasudev
authors: Ajeet
authors: A
authors: Kaushik
authors: Shekhar
authors: S
authors: Bhansali
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D066246:ErbB Receptors / Q000032:analysis* / Q000276:immunology; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D011422:Propionates / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D011422:Propionates; D006046:Gold; D066246:ErbB Receptors