title: Decreased immunoexpression of standard form of CD44 is an independent favourable predictor of nodal metastasis in colorectal carcinoma.
abstract: BACKGROUND
  CD44 is a transmembrane glycoprotein and is associated with cell-matrix and cell-cell interactions. CD44 expression was shown to be relevant to tumour progression in various types of human cancer. The objective of this study was to investigate the relationship between the expression levels of the standard form of CD44 (CD44s), and clinicopathological characteristics in a subset of colorectal carcinomas (CRC).
  
  
  METHODS
  A total of 96 cases of CRC were retrieved from the archives at the Department of Pathology at King Abdulaziz University, Jeddah, KSA. Immunohistochemistry was performed using antibodies to CD44s. A cut-off of <10% of positive neoplastic cells was used to define low expression, 10-50% to define moderate expression and >50% to define extensive expression. Statistical tests were used to determine the association of CD44s with clinicopathological characteristics in a subset of colorectal carcinomas and survival.
  
  
  RESULTS
  Immunostaining results showed that there was no association between C44 immunoexpression and age of patients, tumour grade, depth of invasion, vascular invasion, recurrence and survival. CD44s immunolabelling was found to have an association with nodal metastasis and to be an independent predictor of nodal metastasis.
  
  
  CONCLUSIONS
  Loss of CD44s immunolabelling in CRC is an independent favourable predictor of regional lymph node metastasis. On the other hand, CD44s loss has no significant association with disease recurrence or survival. Extensive in vivo and in vitro molecular studies are required to elucidate the possible mechanistic association of CD44s with tumour initiation, progression, invasion and metastasis in primary CRC, and in nodal and distant metastases.
authors: Department of Pathology King Abdulaziz University. P.O. Box 80205, Jeddah 21589, Saudi Arabia. jalmaghrabi@hotmail.com
authors: Jaudah
authors: J
authors: Al-Maghrabi
authors: Wafaey
authors: W
authors: Gomaa
authors: Abdelbaset
authors: A
authors: Buhmeida
authors: Mohmmad
authors: M
authors: Al-Qahtani
authors: Mahmoud
authors: M
authors: Al-Ahwal
mesh_terms: D002277:Carcinoma / Q000276:immunology / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000276:immunology / Q000473:pathology*; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged
chemical_list: D018960:Hyaluronan Receptors