title: A murine experimental model for the mechanical behaviour of viable right-ventricular myocardium.
abstract: Although right-ventricular function is an important determinant of cardio-pulmonary performance in health and disease, right ventricular myocardium mechanical behaviour has received relatively little attention. We present a novel experimental method for quantifying the mechanical behaviour of transmurally intact, viable right-ventricular myocardium. Seven murine right ventricular free wall (RVFW) specimens were isolated and biaxial mechanical behaviour measured, along with quantification of the local transmural myofibre and collagen fibre architecture. We developed a complementary strain energy function based method to capture the average biomechanical response. Overall, murine RVFW revealed distinct mechanical anisotropy. The preferential alignment of the myofibres and collagen fibres to the apex-to-outflow-tract direction was consistent with this also being the mechanically stiffer axis. We also observed that the myofibre and collagen fibre orientations were remarkably uniform throughout the entire RVFW thickness. Thus, our findings indicate a close correspondence between the tissue microstructure and biomechanical behaviour of the RVFW myocardium, and are a first step towards elucidating the structure–function of non-contracted murine RVFW myocardium in health and disease.
authors: Heart and Vascular Institute, Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
authors: Daniela
authors: D
authors: Valdez-Jasso
authors: Marc A
authors: MA
authors: Simon
authors: Hunter C
authors: HC
authors: Champion
authors: Michael S
authors: MS
authors: Sacks
mesh_terms: D000818:Animals; D006321:Heart / Q000502:physiology*; D008297:Male; D008955:Models, Cardiovascular; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D016278:Ventricular Function, Right / Q000502:physiology*