title: [Effect of shengbanfang on CYP3A1 activities of Sprague-Dawley rat].
abstract: OBJECTIVE
  To study the influences of Shengbanfang on CYP3A1 activities of SD rat and provide suggestions for drug combinations.
  
  
  METHODS
  25 male SD rats were devided into 5 groups randomly,and treated with saline( NS, ig, 10 mg/(kg/d) ,qd,14 d), dexamethasone (DEX, ig, 100 mg/(kg x d), qd, 3 d), high dose of Shengbanfang (HD, ig, 8.645 g/kg, bid, 14 d), middle dose of Shengbanfang (MD, ig, 4.322 g/kg, bid, 14 d) and low dose of Shengbanfang (LD, ig, 2.161 g/kg, bid, 14 d), respectively. The HPLC method was established and validated to determine the productive velocity of 6beta-hydroxytestosterone and measure the activity of CYP3Al.
  
  
  RESULTS
  Under the optimized incubation conditions, the productive rates of 6beta-hydroxytestosterone of HD, MD, LD, NS and DEX, groups were (55.82 +/- 5.97), (65.10 +/- 6.83), (60.89 +/- 6.53), (62.17 +/- 6.55), (126.73 +/- 15.40) micromol/(L x mg pro x min). There were significant differences between Shengbanfang groups compared with dexamethasone group, but there was no significant difference between Shengbanfang groups and the control group (NS).
  
  
  CONCLUSIONS
  Shengbanfang has no induce effect on the enzymic activity of CYP3Al in SD rats.
authors: State Key Laboratory of Oncology in South China, Department of Pharmacy, Tumor Hospital Affiliated to Sun Yat-Sen University, Guangzhou 510060, China. liutao@sysucc.org.cn
authors: Tao
authors: T
authors: Liu
authors: Duo
authors: D
authors: Deng
authors: Zi-chao
authors: ZC
authors: Lin
authors: Ying
authors: Y
authors: Pan
authors: Wen-jing
authors: WJ
authors: Zhou
authors: Wang
authors: W
authors: Zhou
authors: Hong-bing
authors: HB
authors: Huang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D051544:Cytochrome P-450 CYP3A / Q000187:drug effects / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006915:Hydroxytestosterones / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000378:metabolism*; D013921:Thrombocytopenia / Q000139:chemically induced / Q000188:drug therapy
chemical_list: D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D006915:Hydroxytestosterones; D013739:Testosterone; C025591:6 beta-hydroxytestosterone; D003907:Dexamethasone; C465138:Cyp3a23-3a1 protein, rat; D051544:Cytochrome P-450 CYP3A