title: An evaluation of the Xpert MTB/RIF assay and detection of false-positive rifampicin resistance in Mycobacterium tuberculosis.
abstract: Recent reports suggest that false-positive rifampicin resistance may be assigned by the Xpert MTB/RIF assay. We analysed 169 specimens using the MTB/RIF assay. Using culture as the gold standard, we found that the assay had 100% sensitivity and specificity for detecting M. tuberculosis. However, we found that the assay incorrectly assigned rifampicin resistance in 4/13 (31%) of cases.
authors: Department of Clinical Microbiology, Auckland District Health Board, Auckland, New Zealand. dwilliamson@adhb.govt.nz
authors: Deborah A
authors: DA
authors: Williamson
authors: Indira
authors: I
authors: Basu
authors: James
authors: J
authors: Bower
authors: Joshua T
authors: JT
authors: Freeman
authors: Gillian
authors: G
authors: Henderson
authors: Sally A
authors: SA
authors: Roberts
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods*; D024881:Drug Resistance, Bacterial; D005189:False Positive Reactions; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis*
chemical_list: D000995:Antitubercular Agents; D012293:Rifampin