title: Toxic effects of two brominated flame retardants BDE-47 and BDE-183 on the survival and protein expression of the tubificid Monopylephorus limosus.
abstract: The toxic effects of two brominated diphenyl ethers (BDE), BDE-47, and BDE-183, on a benthic oligochaete tubificid, Monopylephorus limosus were studied under laboratory conditions. Investigated responses included survival, growth, and protein expression profiles, at BDE concentrations of 1, 10, 100, and 700 ng/g on a dry soil weight basis, with isooctane as the carrier solvent. Body weight losses among treatments were insignificant after 8 weeks of exposure. The 8-wk LC(50) of BDE-47 and -183 were 2311 and 169 ng/g, respectively. By applying multivariate analysis techniques, protein expression patterns were compared and correlated with stressful sources of long-term culture, carrier solvent, BDE-47 and -183. The treatment of 8-wk 100 ng/g BDE-47 was most closely clustered to the 10 ng/g BDE-183 treatment, based on the 40 examined protein spots. This indicated that BDE-183 was more potent to M. limosus, than was BDE-47. The 2-wk and 8-wk controls clustered into different groups indicating the occurrence of physiological changes due to long-term laboratory culture. Additionally, solvent effect was shown by grouping the isooctane carrier to different clusters. With further characterization by principle component analysis, it was found that the separation was mainly contributed by the 2nd principal-component. And, the primarily inhibitory variation was at spots 2 (UMP-CMP kinase) and 40 (plasma retinol-binding protein precursor) in the 8-wk groups. On the contrary, protein spots 16 (cell division control protein 2 homolog) and 24 (mitochondrial DNA mismatch repair protein) showed stimulatory variation. In all, the observed proteomic responses suggest that BDEs disrupted metabolic function in M. limosus and multivariate analysis tool offers significant potential for the assessment of various stress sources at biochemical level.
authors: Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung 811, Taiwan, ROC.
authors: K H
authors: KH
authors: Chiu
authors: C-R
authors: CR
authors: Lin
authors: H-W
authors: HW
authors: Huang
authors: J
authors: J
authors: Shiea
authors: L L
authors: LL
authors: Liu
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D016000:Cluster Analysis; D005411:Flame Retardants / Q000633:toxicity*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D007928:Lethal Dose 50; D009835:Oligochaeta / Q000187:drug effects*; D011075:Polybrominated Biphenyls / Q000633:toxicity*; D025341:Principal Component Analysis; D012989:Soil Pollutants / Q000633:toxicity*; D016019:Survival Analysis
chemical_list: D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; D011075:Polybrominated Biphenyls; D012989:Soil Pollutants; C511295:2,2',4,4'-tetrabromodiphenyl ether; C483839:2,2',3,4,4',5',6-heptabromodiphenyl ether