title: [Effect of gold nanoparticles on mouse spermatogenesis].
abstract: The response of the mouse male germ cells exposed to gold nanoparticles (approximately 2.5 nm) was studied. Our investigation demonstrates that treatment with Au nanoparticles for four days does not impair the architecture of the spermatogenic epithelium. Cytogenetic evaluation using micronucleus assay showed that gold nanoparticles can affect the chromosomes of early primary spermatocytes. However, gold nanoparticles did not induce chromosome abnormalities in spermatogonial stem cells. Further, the cauda epididymal sperm was isolated on the 14th day after treatment and was incubated in SDS solution (Na sodium dodecyl) and then in a solution containing DTT (dithiothreitol) to induce nuclear chromatin decondensation. Observations showed that after four days of treatment of spermiogenic (postmeiotic) cells with gold nanoparticles the decondensation process had no differences from the control. On the contrary, in the experiment with the same cells and period of fixation but with a single exposure to gold nanoparticles, the number of mature gametes with totally decondensed nuclei reached 100% as opposed to 44% in the controls.
authors: S T
authors: ST
authors: Zakhidov
authors: S M
authors: SM
authors: Pavliuchenkova
authors: T L
authors: TL
authors: Marshak
authors: V M
authors: VM
authors: Rudoĭ
authors: O V
authors: OV
authors: Dement'eva
authors: I A
authors: IA
authors: Zelenina
authors: S G
authors: SG
authors: Skuridin
authors: A A
authors: AA
authors: Makarov
authors: A N
authors: AN
authors: Khokhlov
authors: Iu M
authors: IuM
authors: Evdokimov
mesh_terms: D000818:Animals; D002843:Chromatin / Q000187:drug effects; D004229:Dithiothreitol / Q000494:pharmacology; D004822:Epididymis / Q000166:cytology / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects; D006046:Gold / Q000494:pharmacology*; D008297:Male; D053768:Metal Nanoparticles; D051379:Mice; D008810:Mice, Inbred C57BL; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013091:Spermatogenesis / Q000187:drug effects*; D013093:Spermatogonia / Q000187:drug effects*
chemical_list: D002843:Chromatin; D012967:Sodium Dodecyl Sulfate; D006046:Gold; D004229:Dithiothreitol