title: Antidiabetic effects of Justicia spicigera Schltdl (Acanthaceae).
abstract: BACKGROUND
  Justicia spicigera is a plant species used for the Teenak (Huesteca Potosina) and Mayan (Yucatan peninsula) indigenous for the empirical treatment of diabetes, infections and as stimulant.
  
  
  OBJECTIVE
  To evaluate the cytotoxicity, antioxidant and antidiabetic properties of J. spicigera.
  
  
  METHODS
  The effects of ethanolic extracts of J. spicigera (JSE) on the glucose uptake in insulin-sensitive and insulin-resistant murine 3T3-F442A and human subcutaneous adipocytes was evaluated. The antioxidant activities of the extract of JSE was determined by ABTS and DPPH methods. Additionally, it was evaluated the antidiabetic properties of JSE on T2DM model.
  
  
  RESULTS
  JSE stimulated 2-NBDG uptake by insulin-sensitive and insulin-resistant human and murine adipocytes in a concentration-dependent manner with higher potency than rosiglitazone 1mM. JSE showed antioxidant effects in vitro and induced glucose lowering effects in normoglycemic and STZ-induced diabetic rats.
  
  
  CONCLUSIONS
  The antidiabetic effects of administration of J. spicigera are related to the stimulation of glucose uptake in both insulin-sensitive and insulin-resistant murine and human adipocytes and this evidence justify its empirical use in Traditional Medicine. In addition, J. spicigera exerts glucose lowering effects in normoglycemic and STZ-induced diabetic rats.
authors: Facultad de Química, Universidad Autónoma de Yucatán, Mérida, Yucatán, México. rolffy@uady.mx
authors: Rolffy
authors: R
authors: Ortiz-Andrade
authors: Angel
authors: A
authors: Cabañas-Wuan
authors: Víctor E
authors: VE
authors: Arana-Argáez
authors: Angel Josabad
authors: AJ
authors: Alonso-Castro
authors: Rocio
authors: R
authors: Zapata-Bustos
authors: Luis A
authors: LA
authors: Salazar-Olivo
authors: Fabiola
authors: F
authors: Domínguez
authors: Marco
authors: M
authors: Chávez
authors: Candy
authors: C
authors: Carranza-Álvarez
authors: Alejandro
authors: A
authors: García-Carrancá
mesh_terms: D016475:3T3 Cells; D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives / Q000378:metabolism; D027901:Acanthaceae; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D052160:Benzothiazoles / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D005951:Glucose Tolerance Test; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D044949:Kaempferols / Q000032:analysis; D008297:Male; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D013451:Sulfonic Acids / Q000378:metabolism
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D007004:Hypoglycemic Agents; D044949:Kaempferols; D010936:Plant Extracts; D012997:Solvents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D000431:Ethanol; D003847:Deoxyglucose; D009327:4-Chloro-7-nitrobenzofurazan; D005947:Glucose; C098340:2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose; C042728:lespenefril