title: Protective effects of transduced Tat-DJ-1 protein against oxidative stress and ischemic brain injury.
abstract: Reactive oxygen species (ROS) contribute to the development of a number of neuronal diseases including ischemia. DJ-1, also known to PARK7, plays an important role in transcriptional regulation, acting as molecular chaperone and antioxidant. In the present study, we investigated whether DJ-1 protein shows a protective effect against oxidative stress-induced neuronal cell death in vitro and in ischemic animal models in vivo. To explore DJ-1 protein's potential role in protecting against ischemic cell death, we constructed cell permeable Tat-DJ-1 fusion proteins. Tat-DJ-1 protein efficiently transduced into neuronal cells in a doseand time-dependent manner. Transduced Tat-DJ-1 protein increased cell survival against hydrogen peroxide (H2O2) toxicity and also reduced intracellular ROS. In addition, Tat-DJ-1 protein inhibited DNA fragmentation induced by H2O2. Furthermore, in animal models, immunohistochemical analysis revealed that Tat-DJ-1 protein prevented neuronal cell death induced by transient forebrain ischemia in the CA1 region of the hippocampus. These results demonstrate that transduced Tat-DJ-1 protein protects against cell death in vitro and in vivo, suggesting that the transduction of Tat-DJ-1 may be useful as a therapeutic agent for ischemic injuries related to oxidative stress.
authors: Department of Biomedical Science Research Institute of Bioscience and Biotechnology Hallym University Chunchon 200-702, Korea.
authors: Hoon Jae
authors: HJ
authors: Jeong
authors: Dae Won
authors: DW
authors: Kim
authors: Mi Jin
authors: MJ
authors: Kim
authors: Su Jung
authors: SJ
authors: Woo
authors: Hye Ri
authors: HR
authors: Kim
authors: So Mi
authors: SM
authors: Kim
authors: Hyo Sang
authors: HS
authors: Jo
authors: Hyun Sook
authors: HS
authors: Hwang
authors: Duk Soo
authors: DS
authors: Kim
authors: Sung Woo
authors: SW
authors: Cho
authors: Moo Ho
authors: MH
authors: Won
authors: Kyu Hyung
authors: KH
authors: Han
authors: Jin Seu
authors: JS
authors: Park
authors: Won Sik
authors: WS
authors: Eum
authors: Soo Young
authors: SY
authors: Choi
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005849:Gerbillinae; D047908:Intracellular Signaling Peptides and Proteins / Q000008:administration & dosage* / Q000096:biosynthesis / Q000493:pharmacokinetics; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018696:Neuroprotective Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D015513:Oncogene Proteins / Q000008:administration & dosage* / Q000096:biosynthesis / Q000493:pharmacokinetics; D018384:Oxidative Stress; D016548:Prosencephalon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D000071617:Protein Deglycase DJ-1; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage* / Q000096:biosynthesis / Q000493:pharmacokinetics; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage* / Q000096:biosynthesis / Q000493:pharmacokinetics
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D018696:Neuroprotective Agents; D015513:Oncogene Proteins; D011993:Recombinant Fusion Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D008315:Malondialdehyde; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1