title: Challenges for assessing the performance of biomass degrading biocatalysts.
abstract: Common analytical challenges impact current work to estimate the cost of converting plant biomass to fermentable sugars. The most noteworthy are measuring cellulase and hemicellulase activities, cellulase and hemicellulase protein, biomass compositions (before and after pretreatment), and the products formed. The use of high-throughput (HTP) methods has shown considerable promise for improving both analytical precision and technician efficiency, but can also present pitfalls regarding experimental accuracy and relevance. Recent work demonstrates that HTP systems which include biomass composition analysis, thermal chemical pretreatment, and biomass saccharification can be realized.
authors: National Renewable Energy Laboratory, Biosciences Center, Golden, CO, USA. mike.himmel@nrel.gov
authors: Michael E
authors: ME
authors: Himmel
authors: Stephen R
authors: SR
authors: Decker
authors: David K
authors: DK
authors: Johnson
mesh_terms: D055162:Biocatalysis; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D057166:High-Throughput Screening Assays / Q000379:methods*
chemical_list: D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase