title: Liquid chromatography tandem mass spectrometry determination of chemical markers and principal component analysis of Vitex agnus-castus L. fruits (Verbenaceae) and derived food supplements.
abstract: A validated analytical method for the quantitative determination of seven chemical markers occurring in a hydroalcoholic extract of Vitex agnus-castus fruits by liquid chromatography electrospray triple quadrupole tandem mass spectrometry (LC/ESI/(QqQ)MSMS) is reported. To carry out a comparative study, five commercial food supplements corresponding to hydroalcoholic extracts of V. agnus-castus fruits were analysed under the same chromatographic conditions of the crude extract. Principal component analysis (PCA), based only on the variation of the amount of the seven chemical markers, was applied in order to find similarities between the hydroalcoholic extract and the food supplements. A second PCA analysis was carried out considering the whole spectroscopic data deriving from liquid chromatography electrospray linear ion trap mass spectrometry (LC/ESI/(LIT)MS) analysis. High similarity between the two PCA was observed, showing the possibility to select one of these two approaches for future applications in the field of comparative analysis of food supplements and quality control procedures.
authors: Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, Via Ponte Don Melillo, 84084 Fisciano, Salerno, Italy.
authors: Angela
authors: A
authors: Mari
authors: Paola
authors: P
authors: Montoro
authors: Cosimo
authors: C
authors: Pizza
authors: Sonia
authors: S
authors: Piacente
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid / Q000592:standards; D019587:Dietary Supplements / Q000032:analysis*; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D010946:Plants, Medicinal; D025341:Principal Component Analysis; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000592:standards; D020950:Vitex / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; C553090:Vitex agnus castus extract