title: Drosophila dosage compensation involves enhanced Pol II recruitment to male X-linked promoters.
abstract: Through hyperacetylation of histone H4 lysine 16 (H4K16), the male-specific lethal (MSL) complex in Drosophila approximately doubles transcription from the single male X chromosome in order to match X-linked expression in females and expression from diploid autosomes. By obtaining accurate measurements of RNA polymerase II (Pol II) occupancies and short promoter-proximal RNA production, we detected a consistent, genome-scale increase in Pol II activity at the promoters of male X-linked genes. Moreover, we found that enhanced Pol II recruitment to male X-linked promoters is largely dependent on the MSL complex. These observations provide insights into how global modulation of chromatin structure by histone acetylation contributes to the precise control of Pol II function.
authors: Max Planck Institute of Immunobiology and Epigenetics, 79108 Freiburg im Breisgau, Germany.
authors: Thomas
authors: T
authors: Conrad
authors: Florence M G
authors: FM
authors: Cavalli
authors: Juan M
authors: JM
authors: Vaquerizas
authors: Nicholas M
authors: NM
authors: Luscombe
authors: Asifa
authors: A
authors: Akhtar
mesh_terms: D000107:Acetylation; D000818:Animals; D002460:Cell Line; D047369:Chromatin Immunoprecipitation; D004257:DNA Polymerase II / Q000378:metabolism*; D004303:Dosage Compensation, Genetic; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism*; D005260:Female; D017344:Genes, Insect; D050172:Genes, X-Linked; D006657:Histones / Q000378:metabolism; D008297:Male; D005810:Multigene Family; D011401:Promoter Regions, Genetic; D012727:Sex Characteristics; D014158:Transcription, Genetic; D014960:X Chromosome / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D006657:Histones; D004257:DNA Polymerase II