title: RNAi knockdown of rice SE5 gene is sensitive to the herbicide methyl viologen by the down-regulation of antioxidant defense.
abstract: Plant heme oxygenase (HO) catalyzes the oxygenation of heme to biliverdin, carbon monoxide (CO), and free iron (Fe(2+))-and Arabidopsis and rice (Oryza sativa) HOs are involved in light signaling. Here, we identified that the rice PHOTOPERIOD SENSITIVITY 5 (SE5) gene, which encoded a putative HO with high similarity to HO-1 from Arabidopsis (HY1), exhibited HO activity, and localized in the chloroplasts. Rice RNAi mutants silenced for SE5 were generated and displayed early flowering under long-day conditions, consistent with phenotypes of the null mutation in SE5 gene reported previously (se5 and s73). The herbicide methyl viologen (MV), which produces reactive oxygen species (ROS), was applied to determine whether SE5 regulates oxidative stress response. Compared with wild-type, SE5 RNAi transgenic plants aggravated seedling growth inhibition, chlorophyll loss and ROS overproduction, and decreased the transcripts of some representative antioxidative genes. By contrast, administration of exogenous CO partially rescued corresponding MV hypersensitivity in the SE5 RNAi plants. Alleviation of seed germination inhibition, chlorophyll loss and ROS overproduction, as well as the induction of antioxidant defense were further observed when SE5 or HY1 was overexpressed in transgenic Arabidopsis plants, indicating that SE5 may be useful for molecular breeding designed to improve plant tolerance to oxidative stress.
authors: College of Life Sciences, Cooperative Demonstration Laboratory of Centrifuge Technique, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors: Sheng
authors: S
authors: Xu
authors: Lijuan
authors: L
authors: Wang
authors: Bo
authors: B
authors: Zhang
authors: Bin
authors: B
authors: Han
authors: Yanjie
authors: Y
authors: Xie
authors: Jie
authors: J
authors: Yang
authors: Weigong
authors: W
authors: Zhong
authors: Huiping
authors: H
authors: Chen
authors: Ren
authors: R
authors: Wang
authors: Ning
authors: N
authors: Wang
authors: Weiti
authors: W
authors: Cui
authors: Wenbiao
authors: W
authors: Shen
mesh_terms: D015153:Blotting, Western; D002248:Carbon Monoxide / Q000494:pharmacology; D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects*; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D006540:Herbicides / Q000633:toxicity; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010269:Paraquat / Q000633:toxicity*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013696:Temperature
chemical_list: D006540:Herbicides; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D002734:Chlorophyll; D049452:Green Fluorescent Proteins; D002248:Carbon Monoxide; D006419:Heme Oxygenase (Decyclizing); D010269:Paraquat