title: Mild pretreatment and enzymatic saccharification of cellulose with recycled ionic liquids towards one-batch process.
abstract: The development of second-generation bioethanol involves minimizing the energy input throughout the processing steps. We report here that efficient ionic liquid pretreatments of cellulose can be achieved with short duration times (20 min) at mild temperature (45°C) with [Emim](+)[MeO(H)PO(2)](-) and at room temperature (25 °C) with [Emim](+)[CH(3)COO](-). In these conditions, yields of glucose were increased by a factor of 3. In addition, the recycling of these two imidazolium-based ILs can be performed in maintaining their efficiency to pretreat cellulose. The short time and mild temperature of cellulose solubilization allowed a one-batch processing of [Emim](+)[MeO(H)PO(2)](-) IL-pretreatment and saccharification. In the range from 0 to 100% IL in an aqueous enzymatic medium, the glucose yields were improved at IL proportions between 10 and 40%. The maximum yield at 10% IL is very promising to consider one batch process as efficient as two-step process.
authors: Unité de Génie Enzymatique et Cellulaire, UMR 6022 CNRS, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens Cedex, France.
authors: Thomas
authors: T
authors: Auxenfans
authors: Sébastien
authors: S
authors: Buchoux
authors: Karim
authors: K
authors: Djellab
authors: Carine
authors: C
authors: Avondo
authors: Eric
authors: E
authors: Husson
authors: Catherine
authors: C
authors: Sarazin
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism; D017743:Equipment Reuse; D006868:Hydrolysis; D052578:Ionic Liquids / Q000494:pharmacology*; D011134:Polysaccharides / Q000378:metabolism*; D013696:Temperature
chemical_list: D004798:Enzymes; D052578:Ionic Liquids; D011134:Polysaccharides; D002482:Cellulose