title: Lateral hypothalamic area mediated the protective effects of microinjection of glutamate into interpositus nucleus on gastric ischemia-reperfusion injury in rats.
abstract: We investigated the protective effects of chemical stimulation of cerebellar interpositus nucleus (IN) on gastric ischemia-reperfusion injury (GI-RI) and its possible regulatory mechanisms in rats. Gastric mucosal damage index (GMDI) indicated the severity of gastric mucosal injuries. Transferase dUTP nick end labeling (TUNEL) staining and proliferating cell nuclear antigen (PCNA) were performed to assess gastric mucosal cell apoptosis and proliferation. Microinjection of glutamate into IN markedly attenuated GI-RI. Either chemical lesion of IN or electrical ablation of the decussation of superior cerebellar peduncle (DSCP) obviously aggravated GI-RI. The protective effects of IN were reversed with the pretreatments of microinjection of 3-mercaptopropionic acid into IN or Bicuculline into lateral hypothalamic area (LHA), individually. The discharge frequency and intensity of greater splanchnic nerve (GSN) decreased and gastric mucosal blood flow increased after chemical stimulation of IN. The apoptosis of positive cells of gastric mucosa was decreased by chemical stimulation of IN, whereas proliferation increased. The gastric juice volume, acidity, and total acid output were all decreased after the chemical stimulation of IN. These results indicated that IN participates in regulation of GI-RI and is a specific area in central nervous system for exerting protective effects on GI-RI. DSCP, LHA and GSN may involve in this process. Apoptosis and proliferation may mediate this protective process in rats too.
authors: Department of Gastroenterology, Affiliated Hospital of Xuzhou Medical College, 99 West Huaihai Road, Xuzhou, 221002, Jiangsu, China.
authors: Sheng-Ping
authors: SP
authors: Zhu
authors: Su-Juan
authors: SJ
authors: Fei
authors: Jian-Fu
authors: JF
authors: Zhang
authors: Jin-Zhou
authors: JZ
authors: Zhu
authors: Ying
authors: Y
authors: Li
authors: Zhang-Bo
authors: ZB
authors: Liu
authors: Xiao
authors: X
authors: Qiao
authors: Ting-Ting
authors: TT
authors: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation; D002531:Cerebellum / Q000187:drug effects* / Q000503:physiopathology; D005750:Gastric Juice / Q000737:chemistry / Q000378:metabolism; D005753:Gastric Mucosa / Q000473:pathology / Q000503:physiopathology; D018698:Glutamic Acid / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007026:Hypothalamic Area, Lateral / Q000503:physiopathology*; D008297:Male; D008845:Microinjections; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology* / Q000503:physiopathology; D013270:Stomach / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid