title: Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated             by Epstein-Barr virus latent membrane protein 1.
abstract: We aimed to investigate the relationship between miRNA-146a and latent membrane             protein 1 (LMP1) of Epstein-Barr virus (EBV) in nasopharyngeal carcinoma (NPC).             The expression levels of LMP1 in 40 cases of NPC, 28 cases of chronic nasopharyngitis             and NPC cell lines CNE1 and CNE1-GL (in which LMP1 was stably transfected) were             detected by immunohistochemical staining. The expression of miRNA-146a in 16 cases             of NPC, 13 cases of chronic nasopharyngitis and cell lines was determined by quantitative             reverse transcription polymerase chain reaction (qRT-PCR) analysis. A plasmid             containing the luciferase gene under the control of miRNA-146a promoter (pri-miRNA-146a)             was constructed and transfected into NPC cells, and the luciferase activity was             detected. LMP1 was positive in 17.9% (5/28) of chronic nasopharyngitis cases and             62.5% (25/40) of NPC cases (p<0.01). The miRNA-146a levels in NPC were significantly             higher than that in chronic nasopharyngitis (p<0.01), and were higher in CNE1-GL             cells than those in CNE1 cells (p<0.01). The expression of miRNA-146a in human             NPC was elevated by EBV-associated antigen LMP1, probably through the activation             of the miRNA-146a promoter.
authors: Department of Pathology, Guangdong Medical College, Zhanjiang 524023, PR China.
authors: Yinghai
authors: Y
authors: Zhao
authors: Xiaoyi
authors: X
authors: Chen
authors: Min
authors: M
authors: Jing
authors: Haijun
authors: H
authors: Du
authors: Yi
authors: Y
authors: Zeng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000821:virology; D009304:Nasopharyngitis / Q000235:genetics; D011401:Promoter Regions, Genetic; D015854:Up-Regulation; D014763:Viral Matrix Proteins / Q000378:metabolism*; D055815:Young Adult
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; C542542:MIRN146 microRNA, human; D035683:MicroRNAs; D014763:Viral Matrix Proteins; D008156:Luciferases