title: On-demand killing of adherent cells on photo-acid-generating culture substrates.
abstract: As a powerful tool of cell screening and cell purification, we developed a novel method to kill adherent cells as cultured on a substrate by micro-projection of incoherent visible light. To kill the cells by the mild light irradiated by electrically controllable micro-projection systems currently available, we introduced the assist of the photo-responsive culture substrates functionalized with a photo-acid-generating polymer. In clear contrast to the existing laser-based methods requiring point scanning, areal micro-projection of blue light with the wavelength 436 nm killed many CHO-K1 cells at a time in the irradiated area on the substrate. The effect of the photo-generated acid was so confined that selective killing of targeted cells was achieved without critical damage to the neighboring cells. Further, we demonstrated the photo-selective killing of the adherent cells after preliminarily patterning through the photo-induced removal of cell adhesion-inhibiting polymer.
authors: Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology, Tsukuba Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. k.sumaru@aist.go.jp
authors: Kimio
authors: K
authors: Sumaru
authors: Kyoko
authors: K
authors: Kikuchi
authors: Toshiyuki
authors: T
authors: Takagi
authors: Manae
authors: M
authors: Yamaguchi
authors: Taku
authors: T
authors: Satoh
authors: Kana
authors: K
authors: Morishita
authors: Toshiyuki
authors: T
authors: Kanamori
mesh_terms: D000143:Acids / Q000378:metabolism*; D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion; D016923:Cell Death / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D003584:Cytological Techniques / Q000379:methods*; D008027:Light; D018613:Microscopy, Confocal; D055668:Photochemical Processes / Q000528:radiation effects*; D019904:Polymethyl Methacrylate / Q000378:metabolism / Q000528:radiation effects
chemical_list: D000143:Acids; D019904:Polymethyl Methacrylate