title: Schistosoma mansoni schistosomula tegument (Smteg) immunization in absence of adjuvant induce IL-10 production by CD4+ cells and failed to protect mice against challenge infection.
abstract: The Schistosoma mansoni tegument interaction with the immune system plays a key role in disease establishment or elimination. We have recently demonstrated that S. mansoni schistosomula tegument (Smteg) is able to activate innate immune response and to induce protective immunity in a vaccine formulation with Freunds adjuvant. In this work, we evaluated the ability of Smteg to elicit protection in the absence of adjuvant. Smteg mice immunization resulted in significant antibody production, increased percentage of CD4+IFN-g+ and CD4+IL-10+ cells in spleen and increased production of IFN-g and IL-10 by spleen cells, but failed to reduce parasite burden, female fecundity and morbidity. We also demonstrated that BMDC stimulation with Smteg resulted in significant IL-10 production. Our results demonstrate that Smteg has immune modulatory proprieties.
authors: Centro de Pesquisas René Rachou, Fiocruz-MG, Belo Horizonte, Minas Gerais, Brazil.
authors: Juliano Michel
authors: JM
authors: Araujo
authors: Tatiane Teixeira
authors: TT
authors: de Melo
authors: Isabela Campos
authors: IC
authors: de Sena
authors: Clarice Carvalho
authors: CC
authors: Alves
authors: Neusa
authors: N
authors: Araujo
authors: Fernanda do Valle
authors: Fdo V
authors: Durães
authors: Sergio Costa
authors: SC
authors: Oliveira
authors: Cristina Toscano
authors: CT
authors: Fonseca
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D005298:Fertility / Q000276:immunology; D015801:Helminth Proteins / Q000008:administration & dosage / Q000276:immunology*; D007114:Immunization / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D059208:Parasite Load; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000517:prevention & control*; D014612:Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; C556062:IL10 protein, mouse; D014612:Vaccines; D016753:Interleukin-10; D007371:Interferon-gamma