title: Synthesis, characterizations, and biocompatibility of block poly(ester-urethane)s based on biodegradable poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P3/4HB) and poly(ε-caprolactone).
abstract: A type of block poly(ester-urethane)s (abbreviated as PUBC) based on bacterial copolyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P3/4HB) and biodegradable poly(ε-caprolactone) (PCL) was synthesized by melting polymerization using 1,6-hexamethylene diisocyanate (HDI) as the coupling agent, with different 3HB, 4HB and PCL contents and segment lengths. Stannous octanoate (Sn(Oct)(2)) was used as catalyst. The chemical structure, molecular weight and thermal property were characterized by (1)H NMR, FTIR GPC, DSC and TGA. DSC analysis revealed that the PUBC polyurethanes exhibit amorphous to semi-crystalline (20.9% crystallinity degree) with T(g) range from -39.7 to -21.5 °C. The hydrophilicity was investigated by static contact angle of deionized water and CH(2)I(2). The obtained PUBCs are hydrophobic (water contact angle 73.7-90.2°). Platelet adhesion study and plasma recalcification time revealed that the block polyurethanes possess hemastasis ability. CCK-8 assay illuminated that the no cytotoxic polyurethanes maintain rat aortic smooth muscle cells (RaSMCs) good viability. It was found that the 4HB content in the materials is an important factor to affect the sustainable cell viability.
authors: Multidisciplinary Research Center, Shantou University, Shantou, Guangdong 515063, China.
authors: Handi
authors: H
authors: Qiu
authors: Dandan
authors: D
authors: Li
authors: Xi
authors: X
authors: Chen
authors: Kaiyan
authors: K
authors: Fan
authors: Wenfeng
authors: W
authors: Ou
authors: Kevin C
authors: KC
authors: Chen
authors: Kaitian
authors: K
authors: Xu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D001673:Biodegradation, Environmental; D001792:Blood Platelets / Q000187:drug effects / Q000648:ultrastructure; D002152:Calorimetry, Differential Scanning; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D006885:Hydroxybutyrates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008422:Materials Testing; D008970:Molecular Weight; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D010973:Platelet Adhesiveness / Q000187:drug effects; D011091:Polyesters / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D011140:Polyurethanes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013816:Thermodynamics; D013818:Thermogravimetry; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D006885:Hydroxybutyrates; D011091:Polyesters; D011140:Polyurethanes; C081821:microthane foam; C068056:poly(3-hydroxybutyrate-co-4-hydroxybutyrate); D000094122:Polyhydroxybutyrates; C016240:polycaprolactone