title: Cytologic and immunophenotypic features of CD34+ stem cells in body cavity fluids.
abstract: OBJECTIVE
  The possibility of leakage of CD34+ bone marrow stem cells from the peripheral blood into the coelomic cavity and the capability of coelomic fluid factors to induce their non-hematogenous differentiation were examined by immunocytochemistry (ICC).
  
  
  METHODS
  Body cavity fluid smears from 12 and 18 patients with and without cancer, respectively, were processed for double immunoperoxidase or double fluorescent ICC methods using antibodies against CD34, CD14, CD16, CD68, AE1/AE3, epidermal growth factor receptor (EGFR), D2-40, and CA125.
  
  
  RESULTS
  Heavily irritated exudative fluid from 6 patients with or without cancer contained a few small round cells positive for CD34. Some of them co-expressed myeloid or monocytic markers such as CD14, CD68 or CD16. Some of the CD34+ cells also co-expressed AE1/AE3 or EGFR. In addition, D2-40 and CA125 were also demonstrated though the expression of the latter was quite sporadic.
  
  
  CONCLUSIONS
  These findings support the concept that CD34+ stem cells can be released into irritated body cavity fluid and the possibility of subsequent differentiation to a non-hematogenous lineage under the influence of local humoral factors, in agreement with our previous in vitro experiments. The possibility of such a phenomenon should be kept in mind when body cavity fluid specimens are analyzed by ICC for diagnostic purposes.
authors: Department of Clinical Laboratory, Saiseikai Shiga Hospital, Imperial Gift Foundation, Inc., Shiga, Japan.
authors: Hideo
authors: H
authors: Kataoka
authors: Hidetoshi
authors: H
authors: Okabe
authors: Shigeru
authors: S
authors: Amano
authors: Eiji
authors: E
authors: Yamada
authors: Mitsuaki
authors: M
authors: Ishida
authors: Ryoji
authors: R
authors: Kushima
authors: Tadao K
authors: TK
authors: Kobayashi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000378:metabolism; D001826:Body Fluids / Q000166:cytology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000473:pathology*
chemical_list: D018952:Antigens, CD34