title: Quantification of Yersinia enterocolitica in raw milk using qPCR.
abstract: This report describes a new, sensitive and specific protocol for rapid detection and quantification of Yersinia enterocolitica in artificially contaminated raw milk samples. The new method is based on an optimized real-time PCR protocol with a TaqMan probe. The primers and probe are based on the chromosomal ail gene. This method was successful for both intended uses: (1) direct detection and quantification of Y. enterocolitica in artificially and naturally contaminated raw milk samples and (2) characterization of growth potential of different serotypes of Y. enterocolitica in raw milk at the most commonly used storage temperatures. The recent method eliminates the pre-PCR enrichment step, which makes it possible to quickly assess milk-related consumer exposure to this pathogen.
authors: The Stephan Angeloff Institute of Microbiology, Department of Infectious Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria. hnajdenski@abv.bg
authors: Hristo
authors: H
authors: Najdenski
authors: Marc
authors: M
authors: Heyndrickx
authors: Lieve
authors: L
authors: Herman
authors: Hadewig
authors: H
authors: Werbrouck
authors: Els
authors: E
authors: Van Coillie
mesh_terms: D000818:Animals; D058491:Bacterial Load / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D005511:Food Handling; D005516:Food Microbiology; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013696:Temperature; D015008:Yersinia enterocolitica / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D017931:DNA Primers