title: Characterization of the twin-arginine transport secretome in Sinorhizobium meliloti and evidence for host-dependent phenotypes.
abstract: The twin-arginine transport (Tat) system is essential for cell viability in Sinorhizobium meliloti and may play a role during the development of root nodules. Utilizing an in vivo recombination strategy, we have constructed 28 strains that contain deletions in predicted Tat substrates. Testing of these mutations for symbiotic proficiency on the plant hosts alfalfa and sweet clover shows that some of these mutations affect associations with these hosts differentially.
authors: Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors: Brad S
authors: BS
authors: Pickering
authors: Harry
authors: H
authors: Yudistira
authors: Ivan J
authors: IJ
authors: Oresnik
mesh_terms: D017353:Gene Deletion; D000455:Medicago sativa / Q000382:microbiology; D029903:Melilotus / Q000382:microbiology; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D011995:Recombination, Genetic; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D013559:Symbiosis
chemical_list: D026901:Membrane Transport Proteins