title: Isolation, preliminary characterization, and full-genome analyses of tick-borne encephalitis virus from Mongolia.
abstract: Tick-borne encephalitis virus (TBEV) causes one of the most important inflammatory diseases of the central nervous system, namely severe encephalitis in Europe and Asia. Since the 1980s tick-borne encephalitis is known in Mongolia with increasing numbers of human cases reported during the last years. So far, however, data on TBEV strains are still sparse. We herein report the isolation of a TBEV strain from Ixodes persulcatus ticks collected in Mongolia in 2010. Phylogenetic analysis of the E-gene classified this isolate as Siberian subtype of TBEV. The Mongolian TBEV strain showed differences in virus titers, plaque sizes, and growth properties in two human neuronal cell-lines. In addition, the 10,242 nucleotide long open-reading frame and the corresponding polyprotein sequence were revealed. The isolate grouped in the genetic subclade of the Siberian subtype. The strain Zausaev (AF527415) and Vasilchenko (AF069066) had 97 and 94 % identity on the nucleotide level. In summary, we herein describe first detailed data regarding TBEV from Mongolia. Further investigations of TBEV in Mongolia and adjacent areas are needed to understand the intricate dispersal of this virus.
authors: Department of Virology & Rickettsiology, Bundeswehr Institute of Microbiology, Neuherbergstraße 11, Munich, Germany.
authors: Stefan
authors: S
authors: Frey
authors: Ilona
authors: I
authors: Mossbrugger
authors: Damdin
authors: D
authors: Altantuul
authors: Jigjav
authors: J
authors: Battsetseg
authors: Rendoo
authors: R
authors: Davaadorj
authors: Damdindorj
authors: D
authors: Tserennorov
authors: Tsoodol
authors: T
authors: Buyanjargal
authors: Dashdavaa
authors: D
authors: Otgonbaatar
authors: Lothar
authors: L
authors: Zöller
authors: Stephanie
authors: S
authors: Speck
authors: Roman
authors: R
authors: Wölfel
authors: Gerhard
authors: G
authors: Dobler
authors: Sandra
authors: S
authors: Essbauer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D004669:Encephalitis Viruses, Tick-Borne / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D016679:Genome, Viral; D006801:Humans; D018884:Ixodes / Q000821:virology*; D008969:Molecular Sequence Data; D008986:Mongolia; D016366:Open Reading Frames; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics; D019562:Viral Load; D010948:Viral Plaque Assay
chemical_list: D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus