title: Early childhood infection of Kaposi's sarcoma-associated herpesvirus in Zambian households: a molecular analysis.
abstract: Sub-Saharan Africa is endemic for Kaposi's sarcoma-associated herpesvirus (KSHV) and there is a high rate of early childhood infection; however, the transmission sources are not well characterized. We examined household members as potential KSHV transmission sources to young children in the KSHV-endemic country of Zambia. To this end, we enrolled and followed Zambian households with at least one KSHV-seropositive child and collected longitudinal buccal swab samples. KSHV burden was evaluated and K1 sequences from the children were determined and analyzed for differences to K1 sequences from household members. The K1 sequences were also analyzed for evolution over time. We generated K1 sequences from 31 individuals across 16 households. Nine households contained multiple KSHV-positive members, including at least one child. In six out of the nine households, the child had 100% sequence identity to all household members. However, in two households the child and mother had distinct K1 sequences. In the remaining household, the children were the only KSHV-infected individuals. Furthermore, we report that 1 out of 18 individuals had K1 sequence variation within the timespan analyzed. In our study, we provide evidence that (i) early childhood KSHV transmission occurs from both within and outside the household, (ii) intrahousehold transmission can occur via nonmaternal sources, (iii) viral shedding in the buccal cavity is highly variable and (iv) the dominant K1 sequence within an individual did not rapidly evolve over time. These results are important for developing KSHV intervention strategies.
authors: Nebraska Center for Virology and School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
authors: Landon N
authors: LN
authors: Olp
authors: Danielle M
authors: DM
authors: Shea
authors: Maxine K
authors: MK
authors: White
authors: Clement
authors: C
authors: Gondwe
authors: Chipepo
authors: C
authors: Kankasa
authors: Charles
authors: C
authors: Wood
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D002648:Child; D002675:Child, Preschool; D005191:Family Characteristics; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006566:Herpesviridae Infections / Q000453:epidemiology* / Q000235:genetics / Q000635:transmission / Q000821:virology*; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D007223:Infant; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000821:virology; D014764:Viral Proteins / Q000235:genetics; D055815:Young Adult; D015024:Zambia / Q000453:epidemiology
chemical_list: C111600:K1 protein, Human herpesvirus 8; D014764:Viral Proteins