title: Epimediphine, a novel alkaloid from Epimedium koreanum inhibits acetylcholinesterase.
abstract: A novel aporphine alkaloid was isolated from the leaves of Epimedium koreanum Nakai during activity-guided fractionation in search of compounds with an anticholinesterase activity. The structure of the new compound was assigned as 1,10-methoxy-7-hydroxy-aporphine (1), which we have named epimediphine. Unambiguous (1)H-NMR and (13)C-NMR data for epimediphine are described. Epimediphine inhibited an acetylcholinesterase (AchE) activity in a dose-dependent manner with an IC50 value of 3.1 µM. Meanwhile, tacrine, dehydroevodiamine and physostigmine, which are therapeutic drugs or candidates for AD, exhibited an anti-AchE activity with IC50 values of 0.4, 37.9 and 0.12 µM, respectively.
authors: Pharmaceutical Resources Botany Laboratory, Chung-Ang University, Seoul, Korea.
authors: Xiaodan
authors: X
authors: Zhang
authors: Mihyue
authors: M
authors: Oh
authors: Seulgi
authors: S
authors: Kim
authors: Jimin
authors: J
authors: Kim
authors: Hyeyeon
authors: H
authors: Kim
authors: Sungun
authors: S
authors: Kim
authors: Peter J
authors: PJ
authors: Houghton
authors: Wankyunn
authors: W
authors: Whang
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001060:Aporphines / Q000737:chemistry / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D034181:Epimedium / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D009682:Magnetic Resonance Spectroscopy; D010830:Physostigmine / Q000494:pharmacology; D013619:Tacrine / Q000494:pharmacology
chemical_list: D000470:Alkaloids; D001060:Aporphines; D002800:Cholinesterase Inhibitors; C586165:epimediphine; C081279:aporphine; D013619:Tacrine; C028633:dehydroevodiamine; D010830:Physostigmine; D000110:Acetylcholinesterase