title: Synthesis and characterization of a novel boronic acid-functionalized chitosan polymeric nanosphere for highly specific enrichment of glycopeptides.
abstract: In this study we describe a method for highly specific enrichment of glycopeptides with boronic acid-functionalized chitosan polymeric nanospheres and matrix assisted laser desorption-ionization mass spectrometry (MALDI-MS). This is the first time chitosan has been used to create nanosphere support material for selective enrichment of glycopeptides by modification with glycidyl methacrylate (GMA) and derivatization with 3-aminophenylboronic acid (APB). Due to their multifunctional chemical moieties, these 20-100 nm chitosan-GMA-APB nanospheres have unique properties, such as good dispersibility, good biocompatibility and chemical stability, as well as augmented specificity with glycopeptides. Enrichment conditions were optimized by using trypsin digested glycoprotein horseradish peroxidase. The high specificity of chitosan-GMA-APB nanospheres was demonstrated by effectively enriching glycopeptides from a digest mixture of horseradish peroxidase and nonglycoproteins (bovine serum albumin (BSA)).
authors: Proteomics Laboratory, Medical and Healthy Analytical Center, Peking University, Beijing 100191, China. zouxj@bjmu.edu.cn
authors: Xiajuan
authors: X
authors: Zou
authors: Dan
authors: D
authors: Liu
authors: Lijun
authors: L
authors: Zhong
authors: Bin
authors: B
authors: Yang
authors: Yaxin
authors: Y
authors: Lou
authors: Yuxin
authors: Y
authors: Yin
mesh_terms: D000818:Animals; D001897:Boronic Acids / Q000737:chemistry* / Q000494:pharmacology; D002417:Cattle; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D045424:Complex Mixtures / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry / Q000494:pharmacology; D006020:Glycopeptides / Q000302:isolation & purification*; D006735:Horseradish Peroxidase / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D054118:Nanospheres / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis* / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity
chemical_list: D001897:Boronic Acids; D045424:Complex Mixtures; D004852:Epoxy Compounds; D006020:Glycopeptides; D008689:Methacrylates; D011108:Polymers; D012710:Serum Albumin, Bovine; C028592:3-aminobenzeneboronic acid; D048271:Chitosan; D006735:Horseradish Peroxidase; C007870:glycidyl methacrylate