title: New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells.
abstract: OBJECTIVE
  The eukaryotic cytosolic chaperonin containing TCP-1 (CCT) plays an important role in maintaining cellular homeostasis by assisting the folding of many proteins and is also well known for the critical roles in disease. However, the functions of CCT complex have not been established globally, especially when translocating into nuclear. The purpose of this study is to explore the function of CCT in nuclear and present a strategy in clinical proteomics studies.
  
  
  METHODS
  Blue native polyacrylamide gel electrophoresis (BN-PAGE) combined with mass spectrometry was applied to separate and identify CCT protein complexes.
  
  
  RESULTS
  We isolated the CCT complex in K562 nucleus and identified a novel CCT complex containing 40 protein components involved in protein folding, RNA processing, apoptosis, and cell metabolism. The interactions between four candidate proteins and CCT were confirmed by immunoblotting. Computational biological analyses and independent biochemical assays validated the overall quality of interactions.
  
  
  CONCLUSIONS
  Our results support clues that CCT might play an unexpected role in various biological processes including RNA processing. And we envision future applications for this system searching for new clues of CCT in disease and readily be applied to the clinic.
authors: Beijing Institute of Radiation Medicine, Beijing, China.
authors: Rui
authors: R
authors: Huang
authors: Miao
authors: M
authors: Yu
authors: Chang-Yan
authors: CY
authors: Li
authors: Yi-Qun
authors: YQ
authors: Zhan
authors: Wang-Xiang
authors: WX
authors: Xu
authors: Feifei
authors: F
authors: Xu
authors: Chang-Hui
authors: CH
authors: Ge
authors: Wei
authors: W
authors: Li
authors: Xiao-Ming
authors: XM
authors: Yang
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D056404:Chaperonin Containing TCP-1 / Q000032:analysis / Q000378:metabolism*; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D020014:K562 Cells; D025941:Protein Interaction Mapping
chemical_list: D056404:Chaperonin Containing TCP-1