title: Development of a sensitive GC-C-IRMS method for the analysis of androgens.
abstract: The administration of anabolic steroids is one of the most important issues in doping control and is detectable through a change in the carbon isotopic composition of testosterone and/or its metabolites. Gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS), however, remains a very laborious and expensive technique and substantial amounts of urine are needed to meet the sensitivity requirements of the IRMS. This can be problematic because only a limited amount of urine is available for anti-doping analysis on a broad spectrum of substances. In this work we introduce a new type of injection that increases the sensitivity of GC-C-IRMS by a factor of 13 and reduces the limit of detection, simply by using solvent vent injections instead of splitless injection. This drastically reduces the amount of urine required. On top of that, by only changing the injection technique, the detection parameters of the IRMS are not affected and there is no loss in linearity.
authors: Doping Control Laboratory, Ghent University, Technologiepark 30, Zwijnaarde, Belgium. michael.polet@ugent.be
authors: Michael
authors: M
authors: Polet
authors: Wim
authors: W
authors: Van Gansbeke
authors: Koen
authors: K
authors: Deventer
authors: Peter
authors: P
authors: Van Eenoo
mesh_terms: D000728:Androgens / Q000737:chemistry / Q000652:urine*; D000736:Androstenes / Q000737:chemistry / Q000652:urine*; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007477:Ions / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D010420:Pentanes / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D000728:Androgens; D000736:Androstenes; D007477:Ions; D010420:Pentanes; C033353:pentane