title: Melanogenesis inhibitory and fibroblast proliferation accelerating effects of noroleanane- and oleanane-type triterpene oligoglycosides from the flower buds of Camellia japonica.
abstract: A 28-noroleanane-type triterpene oligoglycoside, camellioside E (4), an oleanane-type triterpene oligoglycoside, camellioside F (5), and the known compounds camelliosides A (1) and D (3) were isolated from a 50% EtOH extract of Camellia japonica flower buds from Korea. The principal constituents (1 and 5) significantly inhibited melanogenesis in theophylline-stimulated B16 melanoma 4A5 cells. Camellioside B (2), a major constituent of C. japonica grown in Japan, showed potent inhibition of melanogenesis [95.0 ± 1.0% (p < 0.01) at 20 μM]. The inhibitory effects of 1, 2, and 5 were stronger than that of the reference compound, arbutin. We believe the melanogenesis inhibitory effects of 2 and 5 are partly related to the proliferation inhibitory effects in B16 melanoma 4A5 cells. Conversely, camelliosides tended to enhance proliferation in normal human neonatal skin fibroblasts. Interestingly, camellioside B (2) significantly accelerated fibroblast proliferation. This biological selectivity could make camellioside B useful for treating skin disorders. Herein, we report the first scientific investigation of a triterpene that displays an inhibitory effect on melanogenesis, but that also has an enhancing effect on fibroblast proliferation.
authors: Kyoto Pharmaceutical University, Misasagi, Kyoto 607-8412, Japan.
authors: Seikou
authors: S
authors: Nakamura
authors: Toshitsugu
authors: T
authors: Moriura
authors: Sunmi
authors: S
authors: Park
authors: Katsuyoshi
authors: K
authors: Fujimoto
authors: Takahiro
authors: T
authors: Matsumoto
authors: Tomoe
authors: T
authors: Ohta
authors: Hisashi
authors: H
authors: Matsuda
authors: Masayuki
authors: M
authors: Yoshikawa
mesh_terms: D000818:Animals; D028244:Camellia / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects*; D035264:Flowers / Q000187:drug effects; D006801:Humans; D008543:Melanins / Q000096:biosynthesis; D008546:Melanoma, Experimental / Q000188:drug therapy*; D051379:Mice; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009828:Oleanolic Acid / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D056910:Republic of Korea; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012867:Skin / Q000166:cytology
chemical_list: D008543:Melanins; D012503:Saponins; C575898:camellioside E; C575897:camellioside F; D009828:Oleanolic Acid