title: Loktanella cinnabarina sp. nov., isolated from a deep subseafloor sediment, and emended description of the genus Loktanella.
abstract: A Gram-stain-negative, aerobic, heterotrophic and salt-tolerant bacterium, designated strain LL-001(T), was isolated from a deep subseafloor sediment in Japanese waters. Cells were non-motile rods and colonies were smooth, convex, circular and vermilion. The conditions for growth were 15-35 °C, pH 5.5-7.5 and 1-8 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain LL-001(T) belonged to the genus Loktanella within the family Rhodobacteraceae of the class Alphaproteobacteria. 16S rRNA gene sequence similarity between strain LL-001(T) and members of the genus Loktanella was 94.5-98.5 %; the highest sequence similarity was with Loktanella hongkongensis UST950701-009P(T). DNA-DNA relatedness between strain LL-001(T) and L. hongkongensis UST950701-009P(T) was 41.5-43.6 %. The DNA G+C content of strain LL-001(T) was 69.3 mol%. On the basis of biochemical features and 16S rRNA gene sequence comparison, strain LL-001(T) is considered to represent a novel species of the genus Loktanella, for which the name Loktanella cinnabarina sp. nov. is proposed. The type strain is LL-001(T) ( = JCM 18161(T) = CECT 8072(T)). The description of the genus Loktanella is also emended.
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Taishi
authors: T
authors: Tsubouchi
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Yasuhiro
authors: Y
authors: Shimane
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Kozue
authors: K
authors: Mori
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Masayuki
authors: M
authors: Miyazaki
authors: Section 1 Geochemical Oceanography, Office of Marine Research Department of Marine Science, Marine Works Japan Ltd, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Akihiro
authors: A
authors: Tame
authors: Section 1 Geochemical Oceanography, Office of Marine Research Department of Marine Science, Marine Works Japan Ltd, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Katsuyuki
authors: K
authors: Uematsu
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Tadashi
authors: T
authors: Maruyama
authors: Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka-shi, Kanagawa 237-0061, Japan.
authors: Yuji
authors: Y
authors: Hatada
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2