title: Effects of radial shock waves therapy on osteoblasts activities.
abstract: Radial shock waves therapy (RSWT) differs from extracorporeal shock waves therapy (ESWT) in that it produces a non-focused wave that is dissipated radially at the skin. Few studies have yet explored the effects of RSWT on bone tissue. Osteoblasts in culture flasks were studied by polymerase chain reaction after treatment with RSW (500 impulses, 0.05 mJ/mm(2)). An inhibited osteoblastogenesis was observed, with a statistically significant reduction in type 1 collagen, osterix, bone sialoprotein and receptor activator NF kappa ligand expression at 24 and 48 h, of osteocalcin at 24, 48 and 72 h, and osteopontin at 48 and 72 h. These findings show that RSWT is not indicated for treatment of delayed fracture union, pseudoarthrosis, and complex regional pain syndrome. The observed reduction in the receptor activator of nuclear factor-kB ligand/osteoprotegerin ratio suggests that it has an inhibiting effect on osteoclastogenesis, which could make it a useful tool for applications in proliferative diseases.
authors: Department of Neuroscience and Organs of Sense, Orthopedics Section, Faculty of Medicine and Surgery, General Hospital, University of Bari, Piazza Giulio Cesare 11, 70124 Bari, Italy. angelanotarnicola@yahoo.it
authors: Angela
authors: A
authors: Notarnicola
authors: Roberto
authors: R
authors: Tamma
authors: Lorenzo
authors: L
authors: Moretti
authors: Alessandra
authors: A
authors: Fiore
authors: Giovanni
authors: G
authors: Vicenti
authors: Alberta
authors: A
authors: Zallone
authors: Biagio
authors: B
authors: Moretti
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000473:pathology / Q000532:radiotherapy; D016723:Bone Remodeling / Q000528:radiation effects; D001862:Bone Resorption; D002454:Cell Differentiation / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000528:radiation effects; D000075202:Contraindications; D005786:Gene Expression Regulation / Q000528:radiation effects; D019220:High-Energy Shock Waves / Q000009:adverse effects; D057086:High-Intensity Focused Ultrasound Ablation; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000528:radiation effects*; D010010:Osteoclasts / Q000166:cytology / Q000528:radiation effects; D010012:Osteogenesis / Q000528:radiation effects; D014176:Protein Biosynthesis / Q000528:radiation effects; D060888:Real-Time Polymerase Chain Reaction; D012886:Skull / Q000166:cytology; D059708:Ultrasonic Surgical Procedures