title: Myeloperoxidase and free iron levels: potential biomarkers for early detection and prognosis of ovarian cancer.
abstract: OBJECTIVE
  The study sought to identify whether a relationship exists between serum myeloperoxidase (MPO) and free iron with stages of ovarian cancer.
  
  
  METHODS
  Serum and tissue samples were collected from women with stages I through IV ovarian cancer, benign gynecologic conditions, inflammation, and healthy controls. Myeloperoxidase ELISA and VITROS Fe Slide assays were used to measure serum and tissue MPO and free iron levels, respectively. Data were analyzed with a one-way ANOVA with post-hoc comparisons (p < 0.05 considered significant).
  
  
  RESULTS
  There was a significant increase in the level of free iron in serum and tissues obtained from stages II-IV as compared to early-stage (stage I) ovarian cancer. There was an overlap between early-stage and inflammation serum MPO levels, however serum free iron levels were significantly higher in early-stage. There was no significant change in serum free iron levels between non-cancer groups. In contrast, there was a significant increase in serum free iron levels in early-stage as compared to non-cancer groups.
  
  
  CONCLUSIONS
  Collectively, these findings clearly indicate a role for the combination of serum MPO and free iron as biomarkers for early detection and prognosis of ovarian cancer.
authors: Department of Obstetrics and Gynecology, The C.S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors: Nicole M
authors: NM
authors: Fletcher
authors: Zhongliang
authors: Z
authors: Jiang
authors: Rouba
authors: R
authors: Ali-Fehmi
authors: Nancy K
authors: NK
authors: Levin
authors: Jimmy
authors: J
authors: Belotte
authors: Michael A
authors: MA
authors: Tainsky
authors: Michael P
authors: MP
authors: Diamond
authors: Husam M
authors: HM
authors: Abu-Soud
authors: Ghassan M
authors: GM
authors: Saed
mesh_terms: D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000097:blood* / Q000378:metabolism; D016022:Case-Control Studies; D055088:Early Detection of Cancer; D005260:Female; D006801:Humans; D007501:Iron / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000097:blood* / Q000201:enzymology / Q000473:pathology*; D009195:Peroxidase / Q000097:blood* / Q000378:metabolism; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D007501:Iron; D009195:Peroxidase