title: Antimicrobial activity of recombinant Pg-AMP1, a glycine-rich peptide from guava seeds.
abstract: Antimicrobial peptides (AMPs) are compounds that act in a wide range of physiological defensive mechanisms developed to counteract bacteria, fungi, parasites and viruses. These molecules have become increasingly important as a consequence of remarkable microorganism resistance to common antibiotics. This report shows Escherichia coli expressing the recombinant antimicrobial peptide Pg-AMP1 previously isolated from Psidium guajava seeds. The deduced Pg-AMP1 open reading frame consists in a 168 bp long plus methionine also containing a His6 tag, encoding a predicted 62 amino acid residue peptide with related molecular mass calculated to be 6.98 kDa as a monomer and 13.96 kDa at the dimer form. The recombinant Pg-AMP1 peptide showed inhibitory activity against multiple Gram-negative (E. coli, Klebsiella pneumonia and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and Staphylococcus epidermides) bacteria. Moreover, theoretical structure analyses were performed in order to understand the functional differences between natural and recombinant Pg-AMP1 forms. Data here reported suggest that Pg-AMP1 is a promising peptide to be used as a biotechnological tool for control of human infectious diseases.
authors: Departamento de Biologia, Programa de Pós-Graduação em Genética e Biotecnologia, Universidade Federal de Juiz de Fora, Campus Universitário, 36036-900, Martelos, Juiz de Fora-MG, Brazil.
authors: Letícia Stephan
authors: LS
authors: Tavares
authors: João Vitor
authors: JV
authors: Rettore
authors: Renata Mendes
authors: RM
authors: Freitas
authors: William Farias
authors: WF
authors: Porto
authors: Ana Paula do Nascimento
authors: AP
authors: Duque
authors: Júnya de Lacorte
authors: Jde L
authors: Singulani
authors: Osmar Nascimento
authors: ON
authors: Silva
authors: Michelle de Lima
authors: Mde L
authors: Detoni
authors: Eveline Gomes
authors: EG
authors: Vasconcelos
authors: Simoni Campos
authors: SC
authors: Dias
authors: Octávio Luiz
authors: OL
authors: Franco
authors: Marcelo de Oliveira
authors: Mde O
authors: Santos
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects; D005998:Glycine / Q000032:analysis*; D007709:Klebsiella / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D031647:Psidium / Q000737:chemistry*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D013210:Staphylococcus / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: C000588960:AMP1 protein, Psidium guajava; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D011994:Recombinant Proteins; D005998:Glycine