title: [Mutants of Burkholderia cenocepacia with a change in synthesis of N-acyl-homoserine lactones--signal molecules of Quorum Sensing regulation].
abstract: By means of plasposon mutagenesis, mutants of Burkholderia cenocepacia 370 with the change in production of N-acyl-homoserine lactones (AHL), signal molecules of the Quorum Sensing system of regulation, were obtained. To localize plasposon insertions in mutant strains, fragments of chromosomal DNA containing plasposons were cloned, adjacent DNA regions sequenced, and a search for homologous nucleotide sequences in the GeneBank was initiated. It has been shown that the insertion of plasposon into gene lon encoding lon proteinase drastically decreases AHL synthesis. Upon insertion of plasposon into gene pps encoding phosphoenolpyruvate-synthase, enhancement of AHL production is observed. In mutant carrying inactivated gene lon, a strong decline of extracellular protease activity, hemolytic, and chitinolytic activities was observed in comparison with the original strain; lipase activity was not changed in this mutant. Mutation in gene pps did not affect these properties of B. cenocepacia 370. Mutations in genes lon and pps reduced the virulence of bacteria upon infection of mice.
authors: M A
authors: MA
authors: Veselova
authors: V A
authors: VA
authors: Lipasova
authors: Iu V
authors: IuV
authors: Zaĭtseva
authors: O A
authors: OA
authors: Koksharova
authors: M Iu
authors: MIu
authors: Chernukha
authors: Iu M
authors: IuM
authors: Romanova
authors: I A
authors: IA
authors: Khmel'
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000818:Animals; D018441:Biofilms; D057508:Burkholderia cenocepacia / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D015964:Gene Expression Regulation, Bacterial; D008297:Male; D051379:Mice; D009154:Mutation; D017866:Phosphotransferases (Paired Acceptors) / Q000235:genetics* / Q000378:metabolism; D049070:Protease La / Q000235:genetics* / Q000378:metabolism; D053038:Quorum Sensing / Q000235:genetics*; D014774:Virulence / Q000235:genetics
chemical_list: D054742:Acyl-Butyrolactones; D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase; D049070:Protease La