title: Quantitative profiling of oxylipins through comprehensive LC-MS/MS analysis: application in cardiac surgery.
abstract: Oxylipins, including eicosanoids, affect a broad range of biological processes, such as the initiation and resolution of inflammation. These compounds, also referred to as lipid mediators, are (non-) enzymatically generated by oxidation of polyunsaturated fatty acids such as arachidonic acid (AA). A plethora of lipid mediators exist which makes the development of generic analytical methods challenging. Here we developed a robust and sensitive targeted analysis platform for oxylipins and applied it in a biological setting, using high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) operated in dynamic multiple reaction monitoring (dMRM). Besides the well-described AA metabolites, oxylipins derived from linoleic acid, dihomo-γ-linolenic acid, α-linolenic acid, eicosapentaenoic acid and docosahexaenoic acid were included. Our comprehensive platform allows the quantitative evaluation of approximately 100 oxylipins down to low nanomolar levels. Applicability of the analytical platform was demonstrated by analyzing plasma samples of patients undergoing cardiac surgery. Altered levels of some of the oxylipins, especially in certain monohydroxy fatty acids such as 12-HETE and 12-HEPE, were observed in samples collected before and 24 h after cardiac surgery. These findings indicate that this generic oxylipin profiling platform can be applied broadly to study these highly bioactive compounds in relation to human disease.
authors: Leiden Amsterdam Centre for Drug Research, Leiden University, Leiden, The Netherlands.
authors: Katrin
authors: K
authors: Strassburg
authors: Annemarie M L
authors: AM
authors: Huijbrechts
authors: Kirsten A
authors: KA
authors: Kortekaas
authors: Jan H
authors: JH
authors: Lindeman
authors: Theresa L
authors: TL
authors: Pedersen
authors: Adrie
authors: A
authors: Dane
authors: Ruud
authors: R
authors: Berger
authors: Arjan
authors: A
authors: Brenkman
authors: Thomas
authors: T
authors: Hankemeier
authors: John
authors: J
authors: van Duynhoven
authors: Eric
authors: E
authors: Kalkhoven
authors: John W
authors: JW
authors: Newman
authors: Rob J
authors: RJ
authors: Vreeken
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D054883:Oxylipins / Q000032:analysis / Q000097:blood*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013903:Thoracic Surgery
chemical_list: D054883:Oxylipins