title: Age-related changes in brain metabolites and cognitive function in APP/PS1 transgenic mice.
abstract: Proton magnetic resonance spectroscopy ((1)H-MRS) and the Morris water maze (MWM) have played an important role in Alzheimer's disease (AD) research. The aim of this study was to determine whether (1)H-MRS and the MWM can detect for early AD in APP/PS1 transgenic (tg) mice. (1)H-MRS was performed in 20 tg mice and 15 wild-type mice at 3, 5 and 8 months of age. The concentration of N-acetylaspartate (NAA), glutamate (Glu), myo-inositol (mI), choline (Cho) and creatine (Cr) in the hippocampus were measured, and the NAA/Cr, Glu/Cr, mI/Cr and Cho/Cr ratios were quantified. Additionally, the spatial learning and memory of the mice were evaluated by MWM. The (1)H-MRS revealed that mI levels in tg mice were significantly higher at 3 months of age compared to wt mice, while the NAA and Glu levels in 5- and 8-month-old tg mice were significantly decreased (p<0.05). Additionally, significant cognitive changes only occurred at 8 months of age in APP/PS1 tg mice. These results indicated that metabolic changes preceded overt cognitive dysfunctions in early-stage AD, suggesting that (1)H-MRS is a more sensitive biomarker for assessing early AD.
authors: Department of Radiology, Suzhou Hospital, Nanjing Medical University, Suzhou 215001, China. sznaonao@163.com
authors: Shuang-qing
authors: SQ
authors: Chen
authors: Qing
authors: Q
authors: Cai
authors: Yu-ying
authors: YY
authors: Shen
authors: Pei-jun
authors: PJ
authors: Wang
authors: Gao-jun
authors: GJ
authors: Teng
authors: Wei
authors: W
authors: Zhang
authors: Feng-chao
authors: FC
authors: Zang
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000523:psychology*; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism / Q000523:psychology; D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D004195:Disease Models, Animal; D059907:Functional Neuroimaging / Q000379:methods / Q000523:psychology; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D007294:Inositol / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018782:Maze Learning / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D053764:Presenilin-1 / Q000235:genetics*; D011522:Protons
chemical_list: D016564:Amyloid beta-Protein Precursor; D015415:Biomarkers; D053764:Presenilin-1; D011522:Protons; D001224:Aspartic Acid; D018698:Glutamic Acid; D007294:Inositol; C000179:N-acetylaspartate; D003401:Creatine; D002794:Choline