title: Monitoring of methylergometrine in human breast milk by solid-phase extraction and high-performance liquid chromatography with fluorimetric detection.
abstract: A high-performance liquid chromatographic assay has been developed for the detection and quantification of the conventional postnatal uterotonic drug, methylergometrine, in human breast milk using a C-18 reversed-phase column by isocratic elution. The analytical method consisted of sample clean-up by solid-phase extraction, and the fluorescence detection required only 8.5 min per sample for separation and quantitation. This assay gave intra- and inter-assay coefficients of variation of less than 7.9% and 7.7%, respectively, and the detection limit was approximately 50 pg/ml. This method was applied for drug level monitoring in the breast milk of patients given methylergometrine.
authors: Department of Hygienic Chemistry, Showa Pharmaceutical University, Tokyo, Japan.
authors: T
authors: T
authors: Nakamichi
authors: A
authors: A
authors: Yawata
authors: H
authors: H
authors: Hojo
authors: H
authors: H
authors: Kagaya
authors: S
authors: S
authors: Kobayashi
authors: T
authors: T
authors: Chikuma
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005470:Fluorometry / Q000379:methods; D006801:Humans; D008755:Methylergonovine / Q000032:analysis* / Q000627:therapeutic use; D008895:Milk, Human / Q000737:chemistry*; D010120:Oxytocics / Q000032:analysis* / Q000627:therapeutic use; D049590:Postpartum Period; D012015:Reference Standards; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods; D012996:Solutions
chemical_list: D010120:Oxytocics; D012996:Solutions; D008755:Methylergonovine