title: Denaturation mapping of Saccharomyces cerevisiae.
abstract: Optical mapping of DNA provides large-scale genomic information that can be used to assemble contigs from next-generation sequencing, and to detect rearrangements between single cells. A recent optical mapping technique called denaturation mapping has the advantage of using physical principles rather than the action of enzymes to probe genomic structure. Denaturation mapping uses fluorescence microscopy to image the pattern of partial melting along a DNA molecule extended in a channel of cross-section 120 nm at the heart of a nanofluidic device. We used denaturation mapping to locate single DNA molecules on the yeast genome (12.1 Mbp) by comparing images to a computationally predicted map for the entire genome sequence. By locating 84 molecules we assembled an optical map of the yeast genome with > 50% coverage.
authors: Department of Physics, McGill University, 3600 rue University, Montreal, Canada.
authors: Robert L
authors: RL
authors: Welch
authors: Robert
authors: R
authors: Sladek
authors: Ken
authors: K
authors: Dewar
authors: Walter W
authors: WW
authors: Reisner
mesh_terms: D002874:Chromosome Mapping; D004271:DNA, Fungal / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D009691:Nucleic Acid Denaturation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal