title: Genome-scale identification of MLO domain-containing genes in soybean (Glycine max L. Merr.).
abstract: In plants, powdery-mildew-resistance locus o (Mlo) genes encode proteins that are calmodulin-binding proteins involved in a variety of cellular processes. However, systematic characterization of this gene family in soybean (Glycine max L. Merr.) has not been yet reported. In this study, we identified MLO domain-contained members in soybean and examined their expression under phytohormone treatment and abiotic stress conditions. A total of 20 soybean Mlo genes were identified (GmMlo1-20), which are distributed on 13 chromosomes, and display diverse exon-intron structures. Phylogenetic analysis indicated that the Mlo family can be classified into four subfamilies. Sequence comparison was used to reveal the conserved calmodulin-binding domain (CaMBD) in GmMLO proteins. The expression of GmMlo genes was influenced by various phytohormone treatments and abiotic stresses, suggesting that these Mlo genes have various roles in the response of soybean to environmental stimuli. Promoter sequence analysis revealed an overabundance of stress and/or phytohormone-related cis-elements in GmMlo genes. These data provide important clues for elucidating the functions of genes of the Mlo gene family.
authors: National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, PR China.
authors: Qi
authors: Q
authors: Shen
authors: Jinming
authors: J
authors: Zhao
authors: Caifu
authors: C
authors: Du
authors: Yang
authors: Y
authors: Xiang
authors: Jinxuan
authors: J
authors: Cao
authors: Xinrong
authors: X
authors: Qin
mesh_terms: D000595:Amino Acid Sequence; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056426:Genetic Loci; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism; D011401:Promoter Regions, Genetic; D054730:Protein Interaction Domains and Motifs; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D030262:Soybean Proteins / Q000235:genetics*; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism
chemical_list: D002148:Calmodulin-Binding Proteins; D010937:Plant Growth Regulators; D030262:Soybean Proteins