title: An improved method for RNA isolation from plants using commercial extraction kits.
abstract: Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.
authors: Warsaw University of Life Sciences-SGGW, Faculty of Horticulture and Landscape Architecture, Department of Plant Pathology, Poland. elzbieta_kalinowska@sggw.pl
authors: Elżbieta
authors: E
authors: Kalinowska
authors: Maria
authors: M
authors: Chodorska
authors: Elżbieta
authors: E
authors: Paduch-Cichal
authors: Karolina
authors: K
authors: Mroczkowska
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004587:Electrophoresis, Agar Gel; D035264:Flowers / Q000235:genetics; D007202:Indicators and Reagents / Q000737:chemistry; D019684:Magnoliopsida / Q000235:genetics*; D024301:Plant Bark / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D007202:Indicators and Reagents; D018749:RNA, Plant