title: Self-assembled complex of probe peptide--E. Coli RNA I conjugate and nano graphene oxide for apoptosis diagnosis.
abstract: Caspase-3 plays an important role in the initiation and propagation of apoptosis which is involved in various kinds of diseases including neurodegenerative diseases and inflammatory diseases. The capase-3 cleavable site of Asp-Glu-Val-Asp (DEVD) connected to a partial sequence of Escherichia coli RNAI was labeled with tetramethyl-6-carboxyrhodamine (TAMRA) as an optical probe. Graphene oxide (GO) was synthesized by a modified Hummer's method and exploited for the preparation of nano-sized GO (NGO) conjugated with polyethylene glycol (PEG). After binding the NGO-PEG by π-π stacking, the quenched fluorescence of TAMRA-DEVD-single stranded DNA (ssDNA) conjugate was recovered via the enzymatic cleavage by caspase-3 in live A549 cells. The comparative study with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay clearly confirmed the specific detection of apoptosis by the non-covalent TAMRA-DEVD-ssDNA/NGO-PEG complex. Furthermore, the self-assembled NGO complex was successfully exploited for in vivo diagnosis of apoptosis-related diseases like hypoxic-ischemic encephalopathy (HIE) and liver cirrhosis.
authors: Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Kyungbuk, South Korea.
authors: Won Ho
authors: WH
authors: Kong
authors: Dong Kyung
authors: DK
authors: Sung
authors: Ki Su
authors: KS
authors: Kim
authors: Ho Sang
authors: HS
authors: Jung
authors: Eun Ji
authors: EJ
authors: Gho
authors: Seok Hyun
authors: SH
authors: Yun
authors: Sei Kwang
authors: SK
authors: Hahn
mesh_terms: D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000175:diagnosis / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics*; D006108:Graphite; D006801:Humans; D000860:Hypoxia / Q000175:diagnosis; D042541:Intracellular Space / Q000201:enzymology; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000175:diagnosis / Q000473:pathology; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000648:ultrastructure; D010455:Peptides; D011092:Polyethylene Glycols / Q000737:chemistry; D012329:RNA, Bacterial; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence
chemical_list: D010455:Peptides; C042027:RNA I; D012329:RNA, Bacterial; D011092:Polyethylene Glycols; D006108:Graphite; D053148:Caspase 3