title: Simple monitoring of cancer cells using nanoparticles.
abstract: Here we present a new strategy for a simple and fast detection of cancer circulating cells (CTCs) using nanoparticles. The human colon adenocarcinoma cell line (Caco2) was chosen as a model CTC. Similarly to other adenocarcinomas, colon adenocarcinoma cells have a strong expression of EpCAM, and for this reason this glycoprotein was used as the capture target. We combine the capturing capability of anti-EpCAM functionalized magnetic beads (MBs) and the specific labeling through antibody-modified gold nanoparticles (AuNPs), with the sensitivity of the AuNPs-electrocatalyzed hydrogen evolution reaction (HER) detection technique. The fully optimized process was used for the electrochemical detection of Caco2 cells in the presence of monocytes (THP-1), other circulating cells that could interfere in real blood samples. Therefore we obtained a novel and simple in situ-like sensing format that we applied for the rapid quantification of AuNPs-labeled CTCs in the presence of other human cells.
authors: Nanobioelectronics & Biosensors Group, Catalan Institute of Nanotechnology, CIN2 (ICN-CSIC), Campus UAB, Barcelona, Spain.
authors: Marisa
authors: M
authors: Maltez-da Costa
authors: Alfredo
authors: A
authors: de la Escosura-Muñiz
authors: Carme
authors: C
authors: Nogués
authors: Lleonard
authors: L
authors: Barrios
authors: Elena
authors: E
authors: Ibáñez
authors: Arben
authors: A
authors: Merkoçi
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000032:analysis / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D015374:Biosensing Techniques; D018938:Caco-2 Cells; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism; D003110:Colonic Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D055664:Electrochemical Techniques; D000071858:Epithelial Cell Adhesion Molecule; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D008856:Microscopy, Fluorescence; D009360:Neoplastic Cells, Circulating / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D006046:Gold