title: Synthesis of biotin labelled cap analogue--incorporable into mRNA transcripts and promoting cap-dependent translation.
abstract: Analogues of the eukaryotic messenger RNA 5' end (m(7)G cap) are useful tools for studying mRNA fate and serve as reagents for in vitro preparation of 5' capped mRNAs. We designed a biotin-labeled dinucleotide cap analogue that can be incorporated into transcripts to produce 5'-capped and biotinylated mRNAs which retain their biological functionality and may be employed for biotin-(strept)avidin technologies.
authors: Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland. jacekj@biogeo.uw.edu.pl
authors: Jacek
authors: J
authors: Jemielity
authors: Maciej
authors: M
authors: Lukaszewicz
authors: Joanna
authors: J
authors: Kowalska
authors: Jakub
authors: J
authors: Czarnecki
authors: Joanna
authors: J
authors: Zuberek
authors: Edward
authors: E
authors: Darzynkiewicz
mesh_terms: D001710:Biotin / Q000737:chemistry*; D015394:Molecular Structure; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D012315:RNA Caps; D012333:RNA, Messenger; D001710:Biotin