title: Influence of sublingual immunotherapy on the expression of Mac-1 integrin in neutrophils from asthmatic children.
abstract: Asthma can be effectively treated with sublingual immunotherapy. The influence of -sublingual immunotherapy on the function of granulocytes in asthmatic patients is largely unknown. Mac-1 integrin is a transmembrane protein containing α (CD11b) and β (CD18) chains. High expression of the complex is found on the surface of neutrophils, NK cells, and macrophages. CD11b/CD18 may bind to CD23, ICAM-1, ICAM-2, and ICAM-4. It plays a crucial role in diapedesis of neutrophils. The aim of the present study was to assess Mac-1 expression on neutrophils from asthmatic children before and after sublingual immunotherapy. Twenty five children aged of 8.1 ± 3.1 suffering from atopic asthma and allergic rhinitis, shortlisted for specific immunotherapy, served as the study group. Fifteen healthy individuals, aged 9.8 ± 3.4, served as a control group. The assessment of CD11b and CD18 expression on cells from peripheral blood was performed with a flow cytometer. The tests were performed before and after 12 months of sublingual immunotherapy. In the asthmatic children, 98.08 (90.79-99.12)% of Mac-1 positive neutrophils were detected. The group was divided into two subgroups: of more than 98% and less than 95% of neutrophils with CD11b/CD18 expression in the sample. After immunotherapy, the percentage of Mac-1 positive granulocytes increased to 99.60 (99.29-99.68)%, p = 0.01. In the control group, 90.56 (87.08-88.86)% granulocytes were Mac-1 positive, p = 0.002. We conclude that sublingual immunotherapy strongly influences the function of the immunological system, including Mac-1 expression on neutrophils.
authors: Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Warsaw Medical University, Warsaw, Poland. olga.ciepiela@wum.edu.pl
authors: Olga
authors: O
authors: Ciepiela
authors: Anna
authors: A
authors: Zawadzka-Krajewska
authors: Iwona
authors: I
authors: Kotula
authors: Beata
authors: B
authors: Pyrzak
authors: Urszula
authors: U
authors: Demkow
mesh_terms: D000286:Administration, Sublingual; D015703:Antigens, CD / Q000378:metabolism; D001249:Asthma / Q000276:immunology*; D039481:CD11b Antigen / Q000378:metabolism; D018821:CD18 Antigens / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002648:Child; D003888:Desensitization, Immunologic; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D008297:Male; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D017455:Receptors, IgE / Q000378:metabolism; D057705:Transendothelial and Transepithelial Migration
chemical_list: D015703:Antigens, CD; D039481:CD11b Antigen; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; C065289:ICAM2 protein, human; C095100:ICAM4 protein, human; D016177:Macrophage-1 Antigen; D017455:Receptors, IgE; D018799:Intercellular Adhesion Molecule-1