title: A pyramid breeding of eight grain-yield related quantitative trait loci based on marker-assistant and phenotype selection in rice (Oryza sativa L.).
abstract: 1000-Grain weight and spikelet number per panicle are two important components for rice grain yield. In our previous study, eight quantitative trait loci (QTLs) conferring spikelet number per panicle and 1000-grain weight were mapped through sequencing-based genotyping of 150 rice recombinant inbred lines (RILs). In this study, we validated the effects of four QTLs from Nipponbare using chromosome segment substitution lines (CSSLs), and pyramided eight grain yield related QTLs. The new lines containing the eight QTLs with positive effects showed increased panicle and spikelet size as compared with the parent variety 93-11. We further proposed a novel pyramid breeding scheme based on marker-assistant and phenotype selection (MAPS). This scheme allowed pyramiding of as many as 24 QTLs at a single hybridization without massive cross work. This study provided insights into the molecular basis of rice grain yield for direct wealth for high-yielding rice breeding.
authors: National Center for Gene Research and Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China.
authors: Guo
authors: G
authors: Zong
authors: Ahong
authors: A
authors: Wang
authors: Lu
authors: L
authors: Wang
authors: Guohua
authors: G
authors: Liang
authors: Minghong
authors: M
authors: Gu
authors: Tao
authors: T
authors: Sang
authors: Bin
authors: B
authors: Han
mesh_terms: D001947:Breeding / Q000379:methods*; D002874:Chromosome Mapping; D005819:Genetic Markers; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010641:Phenotype; D040641:Quantitative Trait Loci; D012639:Seeds / Q000235:genetics* / Q000502:physiology; D012641:Selection, Genetic
chemical_list: D005819:Genetic Markers