title: Beaded plasma clot: a potent sustained-release, drug-delivery system.
abstract: OBJECTIVE
  The aim of the study was to prepare a drug-entrapped, beaded form of blood plasma for possible sustained drug delivery.
  
  
  METHODS
  Blood plasma mixed with various drugs was enriched with CaCl2 and transferred in the form of small droplets on to a glass slide covered with parafilm. Clot formation was induced by incubation at 37 degrees C.
  
  
  RESULTS
  Plasma-bead entrapped tetracycline, amphotericin B and daunorubicin were released gradually in vitro. Crosslinking of the beads with glutaraldehyde decreased the release rate of drugs remarkably. The plasma bead-entrapped cefotaxime administered subcutaneously in mice was released in a slow and sustained fashion and remained in circulation for a longer duration than the antibiotic administered in the free form.
  
  
  CONCLUSIONS
  The plasma beads have potential for the sustained delivery of drugs in vivo, since their preparation does not require additional thrombin or other proteins and can be readily accomplished by using autologous plasma, thereby minimizing the risk of immunological complications.
authors: Interdisciplinary Biotechnology Unit. Aligarh Muslim University, Aligarh 202002, India.
authors: Ejaj
authors: E
authors: Ahmad
authors: Munazza T
authors: MT
authors: Fatima
authors: Mohammad
authors: M
authors: Owais
authors: Mohammad
authors: M
authors: Saleemuddin
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D051379:Mice; D008807:Mice, Inbred BALB C; D010949:Plasma
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations