title: Simultaneous determination of dextromethorphan and dextrophan in rat plasma by LC-MS/MS and its application to a pharmacokinetic study.
abstract: A highly selective and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for quantitating dextromethorphan (DXM) and its metabolite dextrophan (DXO) in rat plasma using pirfenidone as an internal standard. Protein precipitation with acetonitrile was employed for the sample preparation. Chromatographic separation was achieved on a SB-C18 column at 25 degrees C, with a gradient elution programme of which acetonitrile-0.1% formic acid in water as mobile phase. The flow rate was 0.4 mL/min. Detection is carried out by multiple reaction monitoring (MRM) on a ion-trap LC-MS/MS system with an electrospray ionization interface. The assay is linear over the range 1-500 ng/mL for DXM and 1-250 ng/mL for DXO, with a lower limit of quantitation of 1 ng/mL for both. Intra- and inter-day precision of the assay were less than 9.80% and the accuracy were in the range 96.35-106.39%. The developed method was successfully applied to analyze the drug in samples of rat plasma for pharmacokinetic study.
authors: Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.
authors: Renai
authors: R
authors: Xu
authors: Tao
authors: T
authors: Xu
authors: Zhe
authors: Z
authors: Wang
authors: Haichao
authors: H
authors: Zhan
authors: Xiaole
authors: X
authors: Chen
authors: Xianqin
authors: X
authors: Wang
authors: Lufeng
authors: L
authors: Hu
authors: Xiuhua
authors: X
authors: Zhang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003915:Dextromethorphan / Q000097:blood* / Q000493:pharmacokinetics*; D003917:Dextrorphan / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D008297:Male; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013048:Specimen Handling; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007202:Indicators and Reagents; D003917:Dextrorphan; D003915:Dextromethorphan