title: Comparative studies of saponins in 1-3-year-old main roots, fibrous roots, and rhizomes of Panax notoginseng, and identification of different parts and growth-year samples.
abstract: Notoginsenosides R1, R4, Fa, and K (N-R1, N-R4, N-Fa, and N-K), as well as ginsenosides Rg1, Rb1, Rd, Re, Rf, Rg2 and Rh1 (G-Rg1, G-Rb1, G-Rd, G-Re, G-Rf, G-Rg2 and G-Rh1) in 47 Notoginseng samples including 1-, 2- and 3-year-old main roots, rhizomes and fibrous roots of Panax notoginseng were determined by high-performance liquid chromatography-diode array detection method. Total contents (%) of the 11 saponins were 9.82-14.57 for 2-year old and 14.20-16.00 for 3-year-old rhizomes; 2.72-4.50 for 2-year-old and 1.98-4.92 for 3-year-old fibrous roots; 1.75-3.05 for 1-year-old whole roots; and 3.71-8.98 for 2-year-old and 7.03-11.23 for 3-year-old main roots. Contents of most saponins and total content of 11 saponins were in the order 3- >2- >1-year-old main root samples. G-Rf content, sum of G-Rf and G-Rh1 were, respectively, 0.08-0.18 and 0.14-0.32 for 2- or 3-year-old rhizomes, and 0.01-0.07 and 0.03-0.10 for 2- or 3-year-old main roots. Combined contents of N-R1, G-Rg1 and G-Rb1 were 5.78-9.37 in 3-year-old main roots, and 2.99-7.13 in 2-year-old main roots, of which nearly one-third of samples were lower than the limit (5 %) in the Chinese Pharmacopoeia. Those of 2- or 3-year-old fibrous roots (1.47-3.83) and 1-year-old whole roots (1.41-2.44) were much lower than the limit, and were considered not suitable for use as Notoginseng. Two-year-old main roots are not appropriate for collection as Notoginseng. Different parts and growth years of P. notoginseng can be identified from each another according to differences in saponin content.
authors: Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China.
authors: Xiu-Hong
authors: XH
authors: Jia
authors: Chao-Qun
authors: CQ
authors: Wang
authors: Jin-Huai
authors: JH
authors: Liu
authors: Xiao-Wei
authors: XW
authors: Li
authors: Xuan
authors: X
authors: Wang
authors: Ming-Ying
authors: MY
authors: Shang
authors: Shao-Qing
authors: SQ
authors: Cai
authors: Shu
authors: S
authors: Zhu
authors: Katsuko
authors: K
authors: Komatsu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D053723:Panax notoginseng / Q000737:chemistry* / Q000254:growth & development; D018517:Plant Roots / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry*
chemical_list: D012503:Saponins