title: Quartz crystal microbalance analysis of DNA-templated calcium phosphate mineralization.
abstract: A quartz crystal microbalance (QCM) sensor was developed for the quantitation of calcium phosphate mineralization and the assessment of DNA as a template molecule. Inherent advantages of QCM, such as nanogram sensitivity, temporal resolution, surface-based measurements, and flow capabilities, were leveraged in the design of this sensor, and in-line fluidic mixing was used to control precursor reaction. This research shows that DNA, a highly programmable anionic polymer, is able to template and control mineralization of calcium phosphate, with nucleation occurring in less than 15 min and initial rates ranging from 4 to 8 ng/min. FT-IR measurements show mineralized material to be calcium phosphate resembling hydroxyapatite (HAP) when a DNA template is used. DNA is a promising mineralization template, and the QCM proves to be a dynamic technique for a broad range of heterogeneous mineralization experiments in complement to classic, diffusion-limited, end-point analysis techniques.
authors: Emmanuel College, Boston, Massachusetts 02115, United States.
authors: Sunaro C
authors: SC
authors: Ngourn
authors: Hillary A
authors: HA
authors: Butts
authors: Alexander R
authors: AR
authors: Petty
authors: Jamie E
authors: JE
authors: Anderson
authors: Aren E
authors: AE
authors: Gerdon
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques; D002122:Calcium Chloride / Q000378:metabolism; D002130:Calcium Phosphates / Q000378:metabolism*; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D008903:Minerals / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D002130:Calcium Phosphates; D008903:Minerals; D010710:Phosphates; D004247:DNA; C020243:calcium phosphate; D002122:Calcium Chloride; C018279:sodium phosphate