title: Modulation of matrix metalloproteinase activity by EDTA prevents posterior capsular opacification.
abstract: OBJECTIVE
  To evaluate the effect of ethylenediaminetetraacetic acid (EDTA) on posterior capsular opacification (PCO) of rabbits and to assess its effect on intraocular tissues.
  
  
  METHODS
  Modulation of matrix metalloproteinase (MMP) activity in the aqueous following cataract surgery in rabbits and its prevention by different doses of EDTA was determined by zymography. For evaluation of PCO, lensectomized rabbits were intracamerally injected with single dose of either 5 mg EDTA or normal saline. After one month, the degree of PCO was determined by slitlamp biomicroscopy, Miyake-Apple view, and histology of the lens capsule. The effect of EDTA on intra ocular pressure (IOP), corneal endothelial cells, and the retina was evaluated by tonometry, specular microscopy and scanning electron microscopy, and electroretinography. The concentration of EDTA in the aqueous was determined by high performance liquid chromatography (HPLC) at different time points.
  
  
  RESULTS
  The MMP activity was significantly increased in the aqueous of the operated eyes, and EDTA reduced the degree of increase in a dose-dependent manner. EDTA treatment significantly reduced the degree of PCO (p<0.05). Histopathology of the lens capsule showed a reduction in the number of proliferating and migrating cells as well as MMP2 expression in the EDTA-treated eyes. EDTA treatment did not change the IOP; density, morphology and ultrastructure of the corneal endothelial cells; and electroretinography (ERG). EDTA was detectable in the aqueous humor up to 72 h following a single intracameral injection.
  
  
  CONCLUSIONS
  EDTA reduces the degree of PCO by suppressing the MMP activity and it is not toxic to intra ocular structures at the concentration used.
authors: West Bengal University of Animal & Fishery Sciences, Kolkata, India. shazrakon@yahoo.co.in
authors: Sarbani
authors: S
authors: Hazra
authors: Rajdeep
authors: R
authors: Guha
authors: Geram
authors: G
authors: Jongkey
authors: Himangshu
authors: H
authors: Palui
authors: Akhilesh
authors: A
authors: Mishra
authors: Geeta K
authors: GK
authors: Vemuganti
authors: Samar K
authors: SK
authors: Basak
authors: Tapan Kumar
authors: TK
authors: Mandal
authors: Aditya
authors: A
authors: Konar
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects; D058442:Capsule Opacification / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D002387:Cataract Extraction; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D004596:Electroretinography; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D019573:Epithelium, Corneal / Q000166:cytology / Q000187:drug effects; D056965:Injections, Intraocular; D007429:Intraocular Pressure / Q000187:drug effects; D007903:Lens Capsule, Crystalline / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors; D008855:Microscopy, Electron, Scanning; D011183:Postoperative Complications / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D011817:Rabbits
chemical_list: D061965:Matrix Metalloproteinase Inhibitors; D004492:Edetic Acid; D020778:Matrix Metalloproteinase 2