title: Limited genetic diversity of Aerococcus viridans strains isolated from clinical and subclinical cases of bovine mastitis in Slovakia.
abstract: The Aerococcus viridans isolates from bovine mastitis in Slovakia were isolated and characterized by classical microbiological and biochemical, and molecular techniques including IGS-PCR and rep-PCR, ARDRA and 16S rDNA gene sequencing. The substantial variability of antibiotic resistance patterns was observed. The majority of strains were resistant to beta-lactam antibiotics, the resistance to tetracycline was observed in 3 tested strains, resistance to lincomycin was found in 4 strains and practically all tested strains were sensitive to neomycin and ciprofloxacin. While variable at a phenotypic level, no significant genetic variability among A. viridans isolates was detected by molecular DNA based methods. The data obtained suggest that a few A. viridans strains spread among cow's population in Slovak farms.
authors: Faculty of Medicine, Pavol Jozef Safárik University, Trieda SNP 1, 040 11 Kosice, Slovakia.
authors: T
authors: T
authors: Spaková
authors: J
authors: J
authors: Elecko
authors: M
authors: M
authors: Vasil
authors: J
authors: J
authors: Legáth
authors: P
authors: P
authors: Pristas
authors: P
authors: P
authors: Javorský
mesh_terms: D056570:Aerococcus / Q000145:classification* / Q000235:genetics*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D004351:Drug Resistance; D005260:Female; D014644:Genetic Variation; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000382:microbiology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D018154:Slovakia / Q000453:epidemiology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S