title: Differential expression and regulation of angiopoietin-3 in mouse uterus during preimplantation period.
abstract: Angiogenesis is necessary for successful implantation and decidualization. This study was to investigate the differential expression of angiopoietin-3 (Ang-3) in mouse uterus during early pregnancy and its regulation by steroid hormones using in situ hybridization and reverse transcription polymerase chain reaction (RT-PCR). There was no detectable Ang-3 mRNA signal on days 1-5 of pregnancy by in situ hybridization. On day 6 of pregnancy, a low level of Ang-3 mRNA signal was seen in the primary decidua. Ang-3 mRNA expression gradually increased on days 7 and 8 of pregnancy along with the development of decidua, and its expression scope was also expanded. The RT-PCR result indicated that Ang-3 mRNA expression was low on days 1-4 of pregnancy. On day 5, as embryo implanted, Ang-3 mRNA was highly expressed in mouse uterus, and the expression gradually increased on days 6-8 of pregnancy, with peak level on day 8 of pregnancy. Similarly, Ang-3 mRNA was also strongly expressed in decidualized cells under artificial decidualization. Compared with the delayed uterus, a high level of Ang-3 mRNA expression was detected in activated implantation uterus by RT-PCR. In the ovariectomized mouse uterus, Ang-3 mRNA expression increased and reached the highest level at 12 hr after injection of estrogen, progesterone, and estrogen plus progesterone, respectively. These results suggest that Ang-3 may play an important role during the process of mouse decidualization. Both estrogen and progesterone can induce the expression of Ang-3 in ovariectomized mouse uterus.
authors: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, P R China.
authors: Bin
authors: B
authors: Guo
authors: Xue-Ming
authors: XM
authors: Zhang
authors: Shi-Jie
authors: SJ
authors: Li
authors: Xue-Chao
authors: XC
authors: Tian
authors: Shou-Tang
authors: ST
authors: Wang
authors: Dang-Dang
authors: DD
authors: Li
authors: Ju-Xiong
authors: JX
authors: Liu
authors: Zhan-Peng
authors: ZP
authors: Yue
mesh_terms: D042682:Angiopoietins / Q000378:metabolism*; D000818:Animals; D010064:Embryo Implantation / Q000502:physiology; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D000077267:Fulvestrant; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017403:In Situ Hybridization; D051379:Mice; D015735:Mifepristone / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000502:physiology*; D011247:Pregnancy; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014599:Uterus / Q000378:metabolism*
chemical_list: D042682:Angiopoietins; D004965:Estrogen Antagonists; D004967:Estrogens; C118117:angiopoietin 4; D000077267:Fulvestrant; D015735:Mifepristone; D011374:Progesterone; D004958:Estradiol