title: [Prolonged angiotensin-(1-7) infusion inhibits hepatic fibrosis in rats with bile duct ligation].
abstract: OBJECTIVE
  To observe the inhibitory effect of angiotensin-(1-7) on liver fibrosis induced by bile duct ligation in rats.
  
  
  METHODS
  Eighteen Wistar rats were randomized into 3 groups and subject to sham operation, bile duct ligation (BDL), or BDL with angiotensin-(1-7) treatment. An osmotic minipump was implanted intraperitoneally for administration of saline in the sham-operated and BDL groups and angiotensin-(1-7) (25 µg·kg(-1)·h(-1)) in angiotensin-(1-7) treatment group. After a 4-week treatments, the fibrosis score, Masson staining, and hydroxyproline assay were used to evaluate the level of liver fibrosis in the rats, and immunohistochemistry was used to detect expression of α-smooth muscle actin (α-SMA) in the liver tissue.
  
  
  RESULTS
  Compared with BDL group, a 4-week treatment with angiotensin-(1-7) following BDL significantly reduced the fibrosis score (2.33±0.52 vs 5.17±0.75), hydroxyproline content (0.36±0.03 vs 0.52±0.04) and α-SMA expression (54.11±17.55 vs 191.84±31.72) in the liver tissue of the rats (P<0.01).
  
  
  CONCLUSIONS
  Prolonged infusion of angiotensin-(1-7) inhibit the formation of hepatic fibrosis in rats following bile duct ligation.
authors: Department of Emergency Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. mylx99@163.com
authors: Xu
authors: X
authors: Li
authors: Zuowei
authors: Z
authors: Ning
authors: Wei
authors: W
authors: Luo
authors: Wenyong
authors: W
authors: Zhang
authors: Changhui
authors: C
authors: Yu
mesh_terms: D000803:Angiotensin I / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001652:Bile Ducts / Q000601:surgery; D007263:Infusions, Parenteral; D008026:Ligation; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism* / Q000517:prevention & control* / Q000601:surgery; D008297:Male; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010446:Peptide Fragments; D000803:Angiotensin I; C118790:angiotensin I (1-7)