title: Pharmacological evaluation of tea polysaccharides with antioxidant activity in gastric cancer mice.
abstract: Tea polysaccharides were fractionated by Sephadex G-100 gel permeation chromatography. The results showed that tea polysaccharides were mainly composed of TF-1, TF-2 and TF-3. The average molecular weights of TF-1, TF-2 and TF-3 determined by high-performance gel permeation chromatography (HPGPC) and high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) were 231,568 Da, 46,278 Da and 7251 Da, respectively. The monosaccharide composition of Renshen polysaccharides was evaluated by GC. TF-1 was composed of glucose, mannose, xylose with molar ratio of 1:3.2:1.4. TF-2 and TF-3 consisted of glucose, xylose and glucose, xylose, arabinose with molar ratios of 1:1.7 and 1:2.5:0.9, respectively. TF-1 contained mannose as main sugar component and TF-2 was rich in xylose, whereas TF-3 was rich in xylose. In addition, tea polysaccharides could decrease stomach malondialdehyde (MDA) level, serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels, increased serum immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-10 (IL-10) levels, and stomach antioxidant enzymes activities in gastric cancer mice.
authors: Department of General Surgery, Ninth People's Hospital affiliated to Shanghai Jiaotong University, School of Medicine, Shanghai 200011, China.
authors: Jianjun
authors: J
authors: Yang
authors: Bin
authors: B
authors: Chen
authors: Yan
authors: Y
authors: Gu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D002277:Carcinoma / Q000097:blood / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D008769:Methylnitronitrosoguanidine; D051379:Mice; D009374:Neoplasms, Experimental / Q000097:blood / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000097:blood / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D013662:Tea; D008769:Methylnitronitrosoguanidine; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione