title: The role of recombinant epidermal growth factor and serotonin in the             stimulation of tumor growth in a SCCHN xenograft model.
abstract: One challenge of squamous cell carcinoma of the head and neck (SCCHN) chemotherapy             is a small percentage of tumor cells that arrest in the G0 phase of the cell cycle             and are thus not affected by chemotherapy. This could be one reason for tumor             recurrence at a later date. The recruitment of these G0-arresting cells into the             active cell cycle and thus, proliferation, may increase the efficacy of chemotherapeutic             agents. The aim of this study was to investigate whether stimulation with recombinant             epidermal growth factor (EGF) or serotonin leads to an increased tumor cell proliferation             in xenografts. Detroit 562 cells were injected into NMRI-Foxn1nu mice. Treatment             was performed with 15 µg murine or human EGF, or 200 µg serotonin. The control             mice were treated with Lactated Ringer's solution (5 mice/group). Tumor size was             measured on days 4, 8 and 12 after tumor cell injection. The EGF stimulated mice             showed a significantly higher tumor growth compared to the serotonin-stimulated             mice and the untreated controls. In the present study, we show that it is possible             to stimulate tumor cells in xenografts by EGF and thus, enhance cell proliferation,             resulting in a higher tumor growth compared to the untreated control group. In             our future investigations, we plan to include a higher number of mice, an adjustment             of the EGF dosage and cell subanalysis, considering the heterogeneity of SCCHN tumors.
authors: ENT Department, Medical School, Goethe University, Frankfurt                     am Main, Germany.
authors: Christin
authors: C
authors: Geissler
authors: Markus
authors: M
authors: Hambek
authors: Anne
authors: A
authors: Eckardt
authors: Christoph
authors: C
authors: Arnoldner
authors: Marc
authors: M
authors: Diensthuber
authors: Timo
authors: T
authors: Stöver
authors: Jens
authors: J
authors: Wagenblast
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D004815:Epidermal Growth Factor / Q000494:pharmacology* / Q000502:physiology; D066246:ErbB Receptors / Q000378:metabolism; D005260:Female; D006258:Head and Neck Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology*; D012701:Serotonin / Q000494:pharmacology* / Q000502:physiology; D047368:Tumor Burden
chemical_list: D019394:Ki-67 Antigen; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011994:Recombinant Proteins; D012701:Serotonin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors