title: Electric field orientation for gene delivery using high-voltage and low-voltage pulses.
abstract: Electropermeabilization is a biological physical process in response to the presence of an applied electric field that is used for the transfer of hydrophilic molecules such as anticancer drugs or DNA across the plasma membranes of living cells. The molecular processes that support the transfer are poorly known. The aim of our study was to investigate the effect of high-voltage and low-voltage (HVLV) pulses in vitro with different orientations on cell permeabilization, viability and gene transfection. We monitored the permeabilization with unipolar and bipolar HVLV pulses with different train repetition pulses, showing that HVLV pulses increase cell permeabilization and cell viability. Gene transfer was also observed by measuring green fluorescent protein (GFP) expression. The expression was the same for HVLV pulses and electrogenotherapy pulses for in vitro experimentation. As the viability was better preserved for HVLV-pulsed cells, we managed to increase the number of GFP-expressing cells by up to 65% under this condition. The use of bipolar HVLV train pulses increased gene expression to a higher extent, probably by affecting a larger part of the cell surface.
authors: Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, UMR 5089, 205 Route de Narbonne, BP 64182, 31077 Toulouse Cedex, France. orio@ipbs.fr
authors: J
authors: J
authors: Orio
authors: M
authors: M
authors: Coustets
authors: C
authors: C
authors: Mauroy
authors: J
authors: J
authors: Teissie
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival; D006224:Cricetinae; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D049452:Green Fluorescent Proteins