title: Acetate-producing bifidobacteria protect the host from enteropathogenic infection via carbohydrate transporters.
abstract: The human gut harbors a large and diverse community of commensal bacteria. Among them, Bifidobacterium is known to exhibit various probiotic effects including protection of hosts from infectious diseases. We recently discovered that genes encoding an ATP-binding-cassette-type carbohydrate transporter present in certain bifidobacteria contribute to protecting gnotobiotic mice from death induced by enterohemorrhagic Escherichia coli O157:H7. We elucidated the molecular mechanism on lethal infection in mice associated with several bifidobacterial strains by a multi-omics approach combining genomics, transcriptomics and metabolomics. The combined data clearly show that acetate produced by protective bifidobacteria acts in vivo to promote defense functions of the host epithelial cells and thereby protects the host from lethal infection. As demonstrated here, our multi-omics approach provides a powerful strategy for evaluation of host-microbial interactions in the complex gut ecosystem.
authors: Laboratory for Epithelial Immunobiology, RIKEN Research Center for Allergy and Immunology, Yokohama, Japan.
authors: Shinji
authors: S
authors: Fukuda
authors: Hidehiro
authors: H
authors: Toh
authors: Todd D
authors: TD
authors: Taylor
authors: Hiroshi
authors: H
authors: Ohno
authors: Masahira
authors: M
authors: Hattori
mesh_terms: D000085:Acetates / Q000378:metabolism*; D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology*; D019453:Escherichia coli O157 / Q000276:immunology* / Q000472:pathogenicity; D023281:Genomics; D007155:Immunologic Factors / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism*; D055432:Metabolomics; D051379:Mice; D008954:Models, Biological; D059467:Transcriptome
chemical_list: D000085:Acetates; D007155:Immunologic Factors; D026901:Membrane Transport Proteins