title: Label-free molecular analysis of live Neospora caninum tachyzoites in host cells by selective scanning Raman micro-spectroscopy.
abstract: A selective scanning method was used to measure spatially resolved Raman spectra of live Neospora caninum tachyzoites colonizing human brain microvascular-endothelial cells. The technique allowed the detection of nucleic acids, lipids and proteins linked to the parasites and their cellular micro-environment at ∼10× shorter acquisition time compared to raster scanning.
authors: School of Physics and Astronomy, University of Nottingham, UK.
authors: Kenny
authors: K
authors: Kong
authors: Christopher J
authors: CJ
authors: Rowlands
authors: Hany
authors: H
authors: Elsheikha
authors: Ioan
authors: I
authors: Notingher
mesh_terms: D001921:Brain / Q000469:parasitology*; D042783:Endothelial Cells / Q000469:parasitology*; D006790:Host-Parasite Interactions; D006801:Humans; D008055:Lipids / Q000032:analysis; D018688:Neospora / Q000737:chemistry* / Q000254:growth & development; D009696:Nucleic Acids / Q000032:analysis; D015800:Protozoan Proteins / Q000032:analysis; D013059:Spectrum Analysis, Raman
chemical_list: D008055:Lipids; D009696:Nucleic Acids; D015800:Protozoan Proteins