title: A study on mutual interaction between cytokine induced killer cells and umbilical cord-derived mesenchymal cells: Implication for their in-vivo use.
abstract: Recently a number of cellular therapy based-clinical trials have been carried out using mesenchymal stromal cells (MSC) or cytokine-induced-killer (CIK) cells aiming to improve outcome of allogeneic hematopoietic stem cell transplantation. We have isolated MSC from umbilical cord (UC) exploring the interaction between CIK cells and UC-MSC. We found that UC-MSC could suppress CIK cells activity, when co-cultured in a cell-to-cell system. In addition, CIK cells could potentially lyse UC-MSC in a time and ratio dependent manner that could have implications for their in vivo use. Here we provide experimental data on the mutual interaction of CIK cells and UC-MSC, suggesting a negative interference when the two cell types are used in combination. In the light of our observations, when CIK and UC-MSC will be used in clinical trials, timing and sequencing of their infusion should be considered.
authors: Advanced Cellular Therapy Laboratory, Department of Cellular Therapy and Hematology, San Bortolo Hospital, Via Rodolfi 37, 36100 Vicenza, Italy. k.chieregato@inwind.it
authors: Katia
authors: K
authors: Chieregato
authors: Elena
authors: E
authors: Albiero
authors: Silvia
authors: S
authors: Castegnaro
authors: Martina
authors: M
authors: Bernardi
authors: Emanuele S G
authors: ES
authors: d'Amore
authors: Cristina
authors: C
authors: Zanon
authors: Domenico
authors: D
authors: Madeo
authors: Francesco
authors: F
authors: Rodeghiero
authors: Giuseppe
authors: G
authors: Astori
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002450:Cell Communication / Q000276:immunology*; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D018920:Coculture Techniques; D055612:Cytokine-Induced Killer Cells / Q000166:cytology* / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000276:immunology; D006801:Humans; D020014:K562 Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D014470:Umbilical Cord / Q000166:cytology* / Q000276:immunology
chemical_list: D015415:Biomarkers; D016207:Cytokines