title: (+)-Pinoresinol is a putative hypoglycemic agent in defatted sesame (Sesamum indicum) seeds though inhibiting α-glucosidase.
abstract: Defatted sesame seeds have been reported for hypoglycemic effect in mice and T2DM women. An attempted to identify active components responsible for this effect was conducted using α-glucosidase-guided fractionation, resulting in the isolation of various lignans. Of compounds isolated, only (+)-pinoresinol showed inhibitory activity against rat intestinal maltase with an IC(50) value of 34.3 μM. The kinetic study indicated that enzymatic hydrolysis of maltose is inhibited by (+)-pinoresinol through competitive and noncompetitive manners. However, a lower dissociation constant (k(i) 288 M) of EI complex suggested that competitive inhibition is predominant over noncompetitive mode (k'(i) 1342 M).
authors: Natural Product Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Arin
authors: A
authors: Wikul
authors: Thanakorn
authors: T
authors: Damsud
authors: Keita
authors: K
authors: Kataoka
authors: Preecha
authors: P
authors: Phuwapraisirisan
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D005260:Female; D005663:Furans / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D065089:Glycoside Hydrolase Inhibitors; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D007422:Intestines / Q000201:enzymology; D007700:Kinetics; D017705:Lignans / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D051379:Mice; D051381:Rats; D012639:Seeds / Q000737:chemistry; D031685:Sesamum / Q000737:chemistry*; D013237:Stereoisomerism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D005663:Furans; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D017705:Lignans; D000520:alpha-Glucosidases; C103298:pinoresinol