title: [An anti-human ovarian carcinoma and CD3 bispecific single-chain antibody mediates CDR3 spectratype drift of T cell receptor alpha and beta chains].
abstract: OBJECTIVE
  To analyze the drift of T cell receptor (TCR) Vα and Vβ gene family CDR3 spectratype in response to ovarian carcinoma cells mediated by an anti-human ovarian carcinoma/CD3 bispecific single-chain antibody (BHL-1), and explore the mechanism of the bispecific single-chain antibody-mediated T cell immune response.
  
  
  METHODS
  Immunoscopic spectratyping technique was used to analyze the TCR repertoire diversity (CDR3 spectratype distribution) of the T cells from 6 healthy donors before and after stimulation of the cells with human ovarian carcinoma in the presence of BHL-1. The predominant usage of TCR α and Vβ chain CDR3 was analyzed after the stimulation, and sequence analysis was performed for the CDR3 region of the monoclonal T cells.
  
  
  RESULTS
  The spectratypes of Vα and Vβ gene family TCR CDR3 region showed a Gaussian distribution before stimulation of the T cells from the 6 donors. After stimulation of the T cells, CDR3 spectratype drift occurred in the T cells, and some TCR Vα and Vβ families showed an anomalous and oligoclonal expansion. Different CDR3 sequences of the Vα and Vβ gene family TCR were found in the monoclonal T cells stimulated with BHL-1.
  
  
  CONCLUSIONS
  CDR3 spectratype drift occurs in TCR α and Vβ chains of T cells after stimulation with human ovarian carcinoma cells and BHL-1, indicating that the predominant usage of TCR Vα and Vβ families is associated with the specific T cell immune response mediated by BHL-1.
authors: Institute of Molecular Immunology, School of Biotechnology, Southern Medical University, Guangzhou 510515, China.
authors: Wei
authors: W
authors: Luo
authors: Qian
authors: Q
authors: Wen
authors: Mingqian
authors: M
authors: Zhou
authors: Li
authors: L
authors: Ma
mesh_terms: D018033:Antibodies, Bispecific / Q000276:immunology*; D002460:Cell Line; D045744:Cell Line, Tumor; D022801:Complementarity Determining Regions / Q000276:immunology*; D005260:Female; D006801:Humans; D009000:Monocytes / Q000276:immunology; D010051:Ovarian Neoplasms / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000276:immunology*
chemical_list: D018033:Antibodies, Bispecific; D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta; D057127:Single-Chain Antibodies