title: Comparison of two DNA sequence-based typing schemes for the Fusarium solani Species Complex and proposal of a new consensus method.
abstract: Multilocus sequence typing (MLST) is a widely used approach for differentiating microbial isolates presenting many advantages such as easy access through online databases and straightforward interpretation. For the Fusarium solani species complex (FSSC), three gene regions have been widely used to investigate phylogenetic relationships at the interspecific level (ITS-nuLSU, EF1a, RPB2) and a nomenclature system has been proposed for the different known haplotypes. More recently, a MLST scheme was proposed for this species complex based on the polymorphisms of five housekeeping genes (ACC, ICL, GDP, MDP, SOD). Here, we compare the phylogenetic resolution and sequence discriminatory powers of these two sets of loci on 50 epidemiologically unrelated FSSC strains. Although the widely used gene set offers better phylogenetic resolution, the newly developed gene set is slightly better at discriminating isolates using a MLST method. A consensus scheme of eight loci is proposed for typing FSSC strains combining the advantages of the two previous gene sets and offering the best typing efficiency.
authors: Service de Parasitologie-Mycologie, CHU de Nancy, Hôpitaux de Brabois, 11 allée du Morvan, 54511 Vandœuvre-les-Nancy, France. a.debourgogne@chu-nancy.fr
authors: Anne
authors: A
authors: Debourgogne
authors: Cécile
authors: C
authors: Gueidan
authors: Sybren
authors: S
authors: de Hoog
authors: Alain
authors: A
authors: Lozniewski
authors: Marie
authors: M
authors: Machouart
mesh_terms: D016000:Cluster Analysis; D005670:Fusarium / Q000145:classification* / Q000235:genetics*; D058885:Multilocus Sequence Typing / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D010802:Phylogeny