title: A benchmark for chromatin binding measurements in live cells.
abstract: Live-cell measurement of protein binding to chromatin allows probing cellular biochemistry in physiological conditions, which are difficult to mimic in vitro. However, different studies have yielded widely discrepant predictions, and so it remains uncertain how to make the measurements accurately. To establish a benchmark we measured binding of the transcription factor p53 to chromatin by three approaches: fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS) and single-molecule tracking (SMT). Using new procedures to analyze the SMT data and to guide the FRAP and FCS analysis, we show how all three approaches yield similar estimates for both the fraction of p53 molecules bound to chromatin (only about 20%) and the residence time of these bound molecules (∼1.8 s). We also apply these procedures to mutants in p53 chromatin binding. Our results support the model that p53 locates specific sites by first binding at sequence-independent sites.
authors: Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, 20892, Bethesda, MD, USA.
authors: Davide
authors: D
authors: Mazza
authors: Alice
authors: A
authors: Abernathy
authors: Nicole
authors: N
authors: Golob
authors: Tatsuya
authors: T
authors: Morisaki
authors: James G
authors: JG
authors: McNally
mesh_terms: D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D006801:Humans; D007700:Kinetics; D013050:Spectrometry, Fluorescence; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D002843:Chromatin; D016159:Tumor Suppressor Protein p53