title: Loop-mediated isothermal amplification for rapid detection of the causal agents of cassava brown streak disease.
abstract: The causal agents of cassava brown streak disease have recently been identified as Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Primers have been developed for rapid detection of these viruses by reverse transcription loop-mediated isothermal amplification (RT-LAMP). Performance of the RT-LAMP assays compared favourably with published RT-PCR and real-time RT-PCR methods. Furthermore, amplification by RT-LAMP is completed in 40 min and does not require thermal cycling equipment. Modification of the RT-LAMP reactions to use labelled primers allowed rapid detection of amplification products using lateral flow devices containing antibodies specific to the incorporated labels, avoiding the need for fluorescence detection or gel electrophoresis.
authors: The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, United Kingdom. jenny.tomlinson@fera.gsi.gov.uk
authors: J A
authors: JA
authors: Tomlinson
authors: S
authors: S
authors: Ostoja-Starzewska
authors: I P
authors: IP
authors: Adams
authors: D W
authors: DW
authors: Miano
authors: P
authors: P
authors: Abidrabo
authors: Z
authors: Z
authors: Kinyua
authors: T
authors: T
authors: Alicai
authors: M J
authors: MJ
authors: Dickinson
authors: D
authors: D
authors: Peters
authors: N
authors: N
authors: Boonham
authors: J
authors: J
authors: Smith
mesh_terms: D017931:DNA Primers / Q000235:genetics; D002365:Manihot / Q000821:virology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010935:Plant Diseases / Q000821:virology*; D019176:Potyviridae / Q000302:isolation & purification*; D013997:Time Factors; D014773:Virology / Q000379:methods*
chemical_list: D017931:DNA Primers