title: Purification and characterization of a new bacteriocin active against Campylobacter produced by Lactobacillus salivarius SMXD51.
abstract: Strain SMXD51, isolated from chicken ceca and identified as Lactobacillus salivarius, produced a component that inhibits the growth of Gram-positive and Gram-negative bacteria and especially Campylobacter jejuni. The active peptide from the cell-free supernatant of Lb. salivarius SMXD51 was purified in three steps: (i) precipitation with 80% saturated ammonium sulfate, (ii) elution on a reversed phase SPE UPTI-CLEAN cartridge using different concentrations of acetonitrile, (iii) final purification by reversed phase HPLC on a C(18) column. The mode of action of this peptide of 5383.2 Da was identified as bactericidal, and its amino acid composition was established. This new bacteriocin SMXD51 appears potentially very useful to reduce Campylobacter in poultry prior to processing.
authors: LUNAM Université, Oniris, UMR1014 Secalim, BP 82225, Nantes F-44307, France.
authors: Soumaya
authors: S
authors: Messaoudi
authors: Gilles
authors: G
authors: Kergourlay
authors: Michèle
authors: M
authors: Dalgalarrondo
authors: Yvan
authors: Y
authors: Choiset
authors: Mounir
authors: M
authors: Ferchichi
authors: Hervé
authors: H
authors: Prévost
authors: Marie-France
authors: MF
authors: Pilet
authors: Jean-Marc
authors: JM
authors: Chobert
authors: Mohamed
authors: M
authors: Manai
authors: Xavier
authors: X
authors: Dousset
mesh_terms: D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000254:growth & development; D002851:Chromatography, High Pressure Liquid; D007778:Lactobacillus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight
chemical_list: D001430:Bacteriocins