title: Production of mixed-linkage beta-oligosaccharides from lichenan using immobilized Bacillus licheniformis UEB CF lichenase.
abstract: Lichenase from Bacillus licheniformis UEB CF was immobilized on Amberlite IR120 H. The immobilization yield and lichenase activity were 87 and 92.81 % of initial activity, respectively. The immobilized enzyme exhibited a shift in the optimal pH from 5.0 to 3.0, but the activity optimal temperature was not affected. The immobilized enzyme showed a residual activity of 50 % after five uses. It also exhibited high storage stability and retained 50 % of its initial activity after 120 days at 4 °C. The main hydrolysis products yielded from lichenan were trisaccharide and tetrasaccharide. The resulting mixed-linkage beta-oligosaccharides could be used as a special nutriment for lactic bacteria.
authors: Unité enzymes et bioconversion, Biology Department, Sfax National School of Engineers, Sfax University, B.P. 1173, 3038 Sfax Cedex, Tunisia.
authors: Fatma
authors: F
authors: Chaari
authors: Monia
authors: M
authors: Blibech
authors: Fatma
authors: F
authors: Bhiri
authors: Sameh
authors: S
authors: Maktouf
authors: Raoudha
authors: R
authors: Ellouz-Ghorbel
authors: Semia
authors: S
authors: Ellouz-Chaabouni
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D005936:Glucans / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D005936:Glucans; D009844:Oligosaccharides; D006026:Glycoside Hydrolases; C028020:licheninase; C015968:lichenin