title: Coronavirus E protein forms ion channels with functionally and structurally-involved membrane lipids.
abstract: Coronavirus (CoV) envelope (E) protein ion channel activity was determined in channels formed in planar lipid bilayers by peptides representing either the transmembrane domain of severe acute respiratory syndrome CoV (SARS-CoV) E protein, or the full-length E protein. Both of them formed a voltage independent ion conductive pore with symmetric ion transport properties. Mutations N15A and V25F located in the transmembrane domain prevented the ion conductivity. E protein derived channels showed no cation preference in non-charged lipid membranes, whereas they behaved as pores with mild cation selectivity in negatively-charged lipid membranes. The ion conductance was also controlled by the lipid composition of the membrane. Lipid charge also regulated the selectivity of a HCoV-229E E protein derived peptide. These results suggested that the lipids are functionally involved in E protein ion channel activity, forming a protein-lipid pore, a novel concept for CoV E protein ion channel entity.
authors: Department of Physics, Laboratory of Molecular Biophysics, Universitat Jaume I, 12071 Castellón, Spain.
authors: Carmina
authors: C
authors: Verdiá-Báguena
authors: Jose L
authors: JL
authors: Nieto-Torres
authors: Antonio
authors: A
authors: Alcaraz
authors: Marta L
authors: ML
authors: DeDiego
authors: Jaume
authors: J
authors: Torres
authors: Vicente M
authors: VM
authors: Aguilella
authors: Luis
authors: L
authors: Enjuanes
mesh_terms: D000595:Amino Acid Sequence; D028941:Coronavirus 229E, Human / Q000378:metabolism*; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D008969:Molecular Sequence Data; D045473:Severe acute respiratory syndrome-related coronavirus / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000086082:Viroporin Proteins
chemical_list: C501689:E protein, SARS coronavirus; D007473:Ion Channels; D008563:Membrane Lipids; D014759:Viral Envelope Proteins; D000086082:Viroporin Proteins