title: LincRNA-p21 suppresses target mRNA translation.
abstract: Mammalian long intergenic noncoding RNAs (lincRNAs) are best known for modulating transcription. Here we report a posttranscriptional function for lincRNA-p21 as a modulator of translation. Association of the RNA-binding protein HuR with lincRNA-p21 favored the recruitment of let-7/Ago2 to lincRNA-p21, leading to lower lincRNA-p21 stability. Under reduced HuR levels, lincRNA-p21 accumulated in human cervical carcinoma HeLa cells, increasing its association with JUNB and CTNNB1 mRNAs and selectively lowering their translation. With elevated HuR, lincRNA-p21 levels declined, which in turn derepressed JunB and β-catenin translation and increased the levels of these proteins. We propose that HuR controls translation of a subset of target mRNAs by influencing lincRNA-p21 levels. Our findings uncover a role for lincRNA as a posttranscriptional inhibitor of translation.
authors: Laboratory of Molecular Biology and Immunology, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA.
authors: Je-Hyun
authors: JH
authors: Yoon
authors: Kotb
authors: K
authors: Abdelmohsen
authors: Subramanya
authors: S
authors: Srikantan
authors: Xiaoling
authors: X
authors: Yang
authors: Jennifer L
authors: JL
authors: Martindale
authors: Supriyo
authors: S
authors: De
authors: Maite
authors: M
authors: Huarte
authors: Ming
authors: M
authors: Zhan
authors: Kevin G
authors: KG
authors: Becker
authors: Myriam
authors: M
authors: Gorospe
mesh_terms: D001483:Base Sequence; D002268:Carboxypeptidases / Q000235:genetics / Q000378:metabolism; D051959:ELAV Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D059748:Proteolysis; D012323:RNA Processing, Post-Transcriptional; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; D051959:ELAV Proteins; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D051176:beta Catenin; C487931:mirnlet7 microRNA, human; D002268:Carboxypeptidases; C436327:SCPEP1 protein, human