title: Microarray-based identification of Pitx3 targets during Xenopus embryogenesis.
abstract: BACKGROUND
  Unexpected phenotypes resulting from morpholino-mediated translational knockdown of Pitx3 in Xenopus laevis required further investigation regarding the genetic networks in which the gene might play a role. Microarray analysis was, therefore, used to assess global transcriptional changes downstream of Pitx3.
  
  
  RESULTS
  From the large data set generated, selected candidate genes were confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ hybridization.
  
  
  CONCLUSIONS
  We have identified four genes as likely direct targets of Pitx3 action: Pax6, β Crystallin-b1 (Crybb1), Hes7.1, and Hes4. Four others show equivocal promise worthy of consideration: Vent2, and Ripply2 (aka Ledgerline or Stripy), eFGF and RXRα. We also describe the expression pattern of additional and novel genes that are Pitx3-sensitive but that are unlikely to be direct targets.
authors: Department of Biological Sciences, University of Windsor, Windsor, Ontario, Canada.
authors: Lara
authors: L
authors: Hooker
authors: Cristine
authors: C
authors: Smoczer
authors: Farhad
authors: F
authors: KhosrowShahian
authors: Marian
authors: M
authors: Wolanski
authors: Michael J
authors: MJ
authors: Crawford
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D005123:Eye / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D050437:Genes, Developmental / Q000235:genetics*; D017403:In Situ Hybridization; D018878:Limb Buds / Q000196:embryology / Q000378:metabolism; D046228:Microarray Analysis; D013623:Tail / Q000196:embryology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D059467:Transcriptome; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics*
chemical_list: C506549:Pitx3 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins