title: Culture systems: single step.
abstract: Culture media to support development of zygotes to the blastocyst stage is based either on a single medium or sequential (two-step) media. Single medium culture either with or without day 3 renewal is associated with simplified laboratory protocols and lower costs compared with sequential medium. There are currently insufficient clinical data to conclude that one system, either single or sequential, is superior to the other with regard to clinical performance. This chapter summarizes the rationale for use of a single medium, along with supporting animal and human data for culturing embryos in a single medium, renewed or not renewed.
authors: Obstetrics, Gynecology & Reproductive Biology, Brigham and Women's Hospital, Boston, MA, USA.
authors: Ronit
authors: R
authors: Machtinger
authors: Catherine
authors: C
authors: Racowsky
mesh_terms: D000596:Amino Acids / Q000009:adverse effects / Q000494:pharmacology; D000818:Animals; D003470:Culture Media / Q000737:chemistry; D004492:Edetic Acid / Q000009:adverse effects / Q000494:pharmacology; D046149:Embryo Culture Techniques / Q000379:methods*; D005947:Glucose / Q000009:adverse effects / Q000494:pharmacology; D006801:Humans; D000644:Quaternary Ammonium Compounds / Q000009:adverse effects / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D004492:Edetic Acid; D005947:Glucose