title: Irrepressible, truncated auxin response factors: natural roles and applications in dissecting auxin gene regulation pathways.
abstract: The molecularly well-characterized auxin signal transduction pathway involves two evolutionarily conserved families interacting through their C-terminal domains III and IV: the Auxin Response Factors (ARFs) and their repressors the Aux/IAAs, to control auxin-responsive genes, among them genes involved in auxin transport. ( 1)  (,)  ( 2)  We have developed a new genetic tool to study ARF function. Using MONOPTEROS (MP)/ARF5, we have generated a truncated version of MP (MPΔ), ( 3)  which has lost the target domains for repression by Aux/IAA proteins. Besides exploring genetic interactions between MP and Aux/IAAs, we used this construct to trace MP's role in vascular patterning, a previously characterized auxin dependent process. ( 4)  (,)  ( 5)  Here we summarize examples of naturally occurring truncated ARFs and summarize potential applications of truncated ARFs as analytical tools.
authors: Department of Cell and Systems Biology, University of Toronto, Toronto, ON Canada.
authors: Wenzislava
authors: W
authors: Ckurshumova
authors: Naden T
authors: NT
authors: Krogan
authors: Danielle
authors: D
authors: Marcos
authors: Adriana E
authors: AE
authors: Caragea
authors: Thomas
authors: T
authors: Berleth
mesh_terms: D000595:Amino Acid Sequence; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D058526:Plant Vascular Bundle / Q000254:growth & development / Q000378:metabolism; D017384:Sequence Deletion
chemical_list: D007210:Indoleacetic Acids; D010940:Plant Proteins