title: Zinc inhibits high glucose-induced apoptosis in peritoneal mesothelial cells.
abstract: Zinc (Zn) plays an important role in influencing many types of apoptosis. However, its function in apoptosis in peritoneal mesothelial cells (PMCs) remains unknown. Here, we studied the effects of Zn on high glucose (HG)-induced apoptosis in rat PMCs (RPMCs) and examined the underlying molecular mechanisms. We found that Zn supplementation inhibited HG-induced RPMC apoptosis significantly, by attenuating reactive oxygen species (ROS) production, inhibiting HG-induced sFasR and sFasL over-expression, caspase-8 and caspase-3 activation, and inhibiting release of cytochrome c from mitochondria to the cytosol. Further analysis revealed that Zn supplementation facilitated cell survival through activation of the phosphatidylinositol 3-kinase/Akt signaling pathway and MAPK/ERK pathways. These results indicate that Zn can inhibit apoptosis in HG-induced RPMCs by several independent mechanisms, including an indirect antioxidative effect and probably by inhibition of caspase-8 and caspase-3 activation.
authors: Department of Nephrology, The First Affiliated Hospital of China Medical University, 155th Nanjing North Street, Shenyang, Liaoning, 110001, People's Republic of China.
authors: Xiuli
authors: X
authors: Zhang
authors: Dan
authors: D
authors: Liang
authors: Baolei
authors: B
authors: Guo
authors: Lina
authors: L
authors: Yang
authors: Lining
authors: L
authors: Wang
authors: Jianfei
authors: J
authors: Ma
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D061945:Caspase Inhibitors / Q000378:metabolism / Q000494:pharmacology; D020169:Caspases / Q000737:chemistry / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019587:Dietary Supplements; D015536:Down-Regulation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D053222:Fas Ligand Protein / Q000378:metabolism; D006943:Hyperglycemia / Q000378:metabolism*; D009852:Omentum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010537:Peritoneum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D015032:Zinc / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism
chemical_list: D000975:Antioxidants; D061945:Caspase Inhibitors; D004791:Enzyme Inhibitors; D053222:Fas Ligand Protein; C491898:Fas protein, rat; C505662:Faslg protein, rat; D017382:Reactive Oxygen Species; D019014:fas Receptor; D020169:Caspases; D015032:Zinc