title: Flow cytometric detection of anti-AB antibody titers in blood group O recipients of blood group A2 donor kidneys.
abstract: OBJECTIVE
  ABO-incompatible kidney transplantation has been accepted for end-stage renal failure patients who have no ready opportunity for a deceased or living donor. Antibody titration for ABO-incompatible renal transplantation is not only difficult but also lacks conformity among laboratories. Herein we analyzed 20 living related renal transplant couples to detect recipient anti-A2 antibody using flow cytometric analysis.
  
  
  METHODS
  Patients were admitted to our center for renal transplantation between January 1999 and December 2010. All but four of them had undergone a previous renal transplantation from an ABO-compatible donor but experienced graft failure. All donor blood groups were subtyped by our blood bank using a lectin-based dilution assay. To detect recipient anti-A2 antibody titers we used a tube hemagglutination method. A/B antibody titer analysis by flow cytometry incubated serially diluted serum samples with donor erythrocytes. Each analysis was repeated three times over a 2-week period using an older and the last sera simultaneously.
  
  
  RESULTS
  The 13 male and 7 female patients showed our overall mean age of 32 ± 12 years. All patients had panel-reactive antibody levels below 15%. The level of flow cytometric antibody titers did not vary upon repeated analysis (P = .01). When compared with the tube method there was a discrepancy of the level at which the antibody titer became negative.
  
  
  CONCLUSIONS
  Flow cytometric antibody titration is a practical and rapid technique to determine the amount of anti-A2 antibody in renal recipients.
authors: Department of Molecular Genetics, Haydarpasa Numune Research and Training Hospital, Genetic Diseases Diagnosis Center, Turkey. pinaren@gmail.com
authors: P
authors: P
authors: Ata
authors: F
authors: F
authors: Cetinkaya
authors: T
authors: T
authors: Ozgezer
authors: L
authors: L
authors: Ozel
authors: A
authors: A
authors: Tulunay
authors: E
authors: E
authors: Eksioglu
authors: M I
authors: MI
authors: Titiz
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000328:Adult; D000906:Antibodies / Q000097:blood*; D001787:Blood Group Incompatibility / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006386:Hemagglutination Tests; D006648:Histocompatibility; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D011237:Predictive Value of Tests; D013997:Time Factors; D016896:Treatment Outcome; D014421:Turkey; D055815:Young Adult
chemical_list: D000017:ABO Blood-Group System; D000906:Antibodies