title: Anti-Candida albicans action of the glyco-protein complex purified from metabolites of gut bacterium Raoultella ornithinolytica isolated from earthworms Dendrobaena veneta.
abstract: OBJECTIVE
  The aim of our research was to isolate the compounds from the metabolites of Raoultella ornithinolytica with the activity against Candida albicans and to analyse the action of the compounds on the metabolic activity and morphology of the fungus cells.
  
  
  RESULTS
  The effect of active protein fractions on the cell morphology, growth, and metabolic activity of C. albicans was analysed under a light microscope with Nomarski contrast and after staining with calcofluor-white. The LIVE/DEAD Yeast Viability Kit F-7030 FUN 1 was used for determination of C. albicans metabolic activity. The biomolecules obtained after isolation by ion exchange chromatography were further fractionated by Sephadex G-50 medium gel filtration. Then, after molecular sieve, the fractions were analysed by FTIR and SERS Spectroscopy. A subfraction was isolated from the antifungal protein fraction above 100 kDa. The active subfraction identified as the glyco-protein complex caused a decrease in the metabolic activity and morphological changes of C. albicans cells.
  
  
  CONCLUSIONS
  The glyco-protein complex obtained from metabolites of bacteria Raoultella ornithinolytica possesses antifungal activity against C. albicans and shows minimal toxicity (1%) against fibroblasts.
  
  
  CONCLUSIONS
  Studies on the glyco-protein complex obtained from earthworm gut bacteria R. ornithinolytica can lead to their application in biological fungicide and pharmaceutical industry.
authors: Department of Immunobiology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland. mfiolka@poczta.onet.pl
authors: M J
authors: MJ
authors: Fiołka
authors: K
authors: K
authors: Grzywnowicz
authors: K
authors: K
authors: Chlebiej
authors: E
authors: E
authors: Szczuka
authors: E
authors: E
authors: Mendyk
authors: R
authors: R
authors: Keller
authors: J
authors: J
authors: Rzymowska
mesh_terms: D000666:Amphotericin B; D000818:Animals; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000494:pharmacology; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects*; D004755:Enterobacteriaceae / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009835:Oligochaeta / Q000382:microbiology*; D013059:Spectrum Analysis, Raman
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D006023:Glycoproteins; D000666:Amphotericin B