title: Immunochromatographic assay of cadmium levels in oysters.
abstract: Oysters are one of foodstuffs containing a relatively high amount of cadmium. Here we report on establishment of an immunochromatographic assay (ICA) method of cadmium levels in oysters. Cadmium was extracted with 0.l mol L(-1) HCl from oysters and cleaned up from other metals by the use of an anion-exchange column. The behavior of five metals Mn, Fe, Cu, Zn, and Cd was monitored at each step of extraction and clean-up procedure for the ICA method in an inductively coupled plasma-mass spectrometry (ICP-MS) analysis. The results revealed that a simple extraction method with the HCl solution was efficient enough to extract almost all of cadmium from oysters. Clean-up with an anion-exchange column presented almost no loss of cadmium adsorbed on the column and an efficient removal of metals other than cadmium. When a spiked recovery test was performed in the ICA method, the recovery ranged from 98% to 112% with relative standard deviations between 5.9% and 9.2%. The measured values of cadmium in various oyster samples in the ICA method were favorably correlated with those in ICP-MS analysis (r(2)=0.97). Overall results indicate that the ICA method established in the present study is an adequate and reliable detection method for cadmium levels in oysters.
authors: Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan. knishi@agr.ehime-u.ac.jp
authors: Kosuke
authors: K
authors: Nishi
authors: In-Hae
authors: IH
authors: Kim
authors: Takaaki
authors: T
authors: Itai
authors: Takuya
authors: T
authors: Sugahara
authors: Haruko
authors: H
authors: Takeyama
authors: Hideo
authors: H
authors: Ohkawa
mesh_terms: D000818:Animals; D002104:Cadmium / Q000032:analysis* / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods* / Q000592:standards; D002852:Chromatography, Ion Exchange; D004785:Environmental Pollutants / Q000032:analysis* / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis*; D006851:Hydrochloric Acid / Q000737:chemistry; D010125:Ostreidae / Q000737:chemistry*; D012015:Reference Standards
chemical_list: D004785:Environmental Pollutants; D002104:Cadmium; D006851:Hydrochloric Acid