title: Chronic Myelogenous Leukemia Cells Contribute to the Stromal Myofibroblasts in Leukemic NOD/SCID Mouse In Vivo.
abstract: We recently reported that chronic myelogenous leukemia (CML) cells converted into myofibroblasts to create a microenvironment for proliferation of CML cells in vitro. To analyze a biological contribution of CML-derived myofibroblasts in vivo, we observed the characters of leukemic nonobese diabetes/severe combined immunodeficiency (NOD/SCID) mouse. Bone marrow nonadherent mononuclear cells as well as human CD45-positive cells obtained from CML patients were injected to the irradiated NOD/SCID mice. When the chimeric BCR-ABL transcript was demonstrated in blood, human CML cells were detected in NOD/SCID murine bone marrow. And CML-derived myofibroblasts composed with the bone marrow-stroma, which produced significant amounts of human vascular endothelial growth factor A. When the parental CML cells were cultured with myofibroblasts separated from CML cell-engrafted NOD/SCID murine bone marrow, CML cells proliferated significantly. These observations indicate that CML cells make an adequate microenvironment for their own proliferation in vivo.
authors: Department of Hematology/Oncology, Teikyo University School of Medicine, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8606, Japan.
authors: Ryosuke
authors: R
authors: Shirasaki
authors: Haruko
authors: H
authors: Tashiro
authors: Yoko
authors: Y
authors: Oka
authors: Takuji
authors: T
authors: Matsuo
authors: Tadashi
authors: T
authors: Yamamoto
authors: Toshihiko
authors: T
authors: Sugao
authors: Nobu
authors: N
authors: Akiyama
authors: Kazuo
authors: K
authors: Kawasugi
authors: Naoki
authors: N
authors: Shirafuji