title: A study of a population of Nyssomyia trapidoi (Diptera: Psychodidae) caught on the Pacific coast of Ecuador.
abstract: BACKGROUND
  Cutaneous leishmaniasis is endemic to the Pacific coast of Ecuador, and Nyssomyia trapidoi is considered to be its main vector. Dujardin et al. [1] recorded some differences in body pigmentation and isoenzymatic profiles in sympatric populations of Ny. trapidoi from the Pacific coast of Ecuador and suggested the existence of two cryptic species.
  
  
  METHODS
  Entomological collections were performed in November 2008 and March 2011 in the locality of Paraíso Escondido using CDC miniature light traps and human bait. Morphological, isoenzymatical and molecular (sequencing of cytochome b and cytochrome c oxidase 1 of the mitochondrial DNA) analyses, such as detection of Leishmania DNA and phlebovirus RNA in some females, were performed.
  
  
  RESULTS
  Neighbor-joining trees from mitochondrial sequences grouped all of Ecuadorian Ny. trapidoi (including the two color variants) in one cluster, except for two specimens which clustered separately in both genes. Isoenzymatic characterization confirmed that the color variants belong to the same population. Additionally, 11.5% of females were found by PCR to contain Endotrypanum monterogeii kinetoplastid DNA. All pools of Ny. trapidoi were negative for phlebovirus RNA.
  
  
  CONCLUSIONS
  Analysis of mitochondrial gene sequences and isoenzymes was unable to support the existence of two sibling species within Ny. trapidoi, which is a probable vector of Endotrypanum monterogeii.
authors: Université de Reims Champagne-Ardenne, ANSES, EA 4688 USC Transmission vectorielle et épidémiosurveillance de maladies parasitaires, VECPAR, France.
authors: S
authors: S
authors: Zapata
authors: L
authors: L
authors: Mejía
authors: F
authors: F
authors: Le Pont
authors: R
authors: R
authors: León
authors: B
authors: B
authors: Pesson
authors: C
authors: C
authors: Ravel
authors: L
authors: L
authors: Bichaud
authors: R
authors: R
authors: Charrel
authors: C
authors: C
authors: Cruaud
authors: G
authors: G
authors: Trueba
authors: J
authors: J
authors: Depaquit
mesh_terms: D000483:Alleles; D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics; D003710:Demography; D004484:Ecuador; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D007521:Isocitrate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes; D008291:Malate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D016856:Phlebovirus / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011576:Psychodidae / Q000145:classification* / Q000235:genetics / Q000502:physiology*; D014345:Trypanosoma / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004272:DNA, Mitochondrial; D007527:Isoenzymes; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase