title: Analysis of volatile components in Curcuma rhizome by microemulsion electrokinetic chromatography.
abstract: Volatile chemicals are a group of very important compounds in natural products. Curcuma rhizome, which contains many bioactive volatile compounds, is a traditional Chinese medicine that has long been used for the treatment of several diseases. In the present study, a microemulsion electrokinetic chromatography (MEEKC) method was developed for the analysis of four volatile components in Curcuma rhizome, including germacrone, furanodiene, curcumenol and curdione. Experimental parameters, including the pH, type and concentrations of background electrolyte, and microemulsion compositions (type and concentrations of surfactant, co-surfactant and oil phase) were intensively investigated. Finally, the primary compounds in the methanol extract of Curcuma rhizome were separated within 30 min using a running buffer composed of 2.31% w/v (80 mmol/L) sodium dodecyl sulfate (SDS), 0.91% w/v (80 mmol/L) 1-octane, 6.95% w/v (937.5 mmol/L) 1-butanol and 1.88% w/v (312.5 mmol/L) propanol in a 5-mM borate buffer (pH 8.1). The contents of the four investigated compounds were determined in the rhizome from C. phaeocaulis. The results showed that the developed MEEKC method provided an alternative tool for the analysis of volatile components, especially those of heat-sensitive compounds from natural products.
authors: College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China.
authors: Feng-Qing
authors: FQ
authors: Yang
authors: Jing
authors: J
authors: Yang
authors: Xue-Mei
authors: XM
authors: Zhang
authors: Pan
authors: P
authors: Xu
authors: Zhi-Ning
authors: ZN
authors: Xia
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D030024:Curcuma / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005663:Furans / Q000032:analysis / Q000737:chemistry; D006574:Heterocyclic Compounds, 2-Ring / Q000032:analysis / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D009795:Octanes / Q000737:chemistry; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D012717:Sesquiterpenes / Q000032:analysis* / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D005663:Furans; D006574:Heterocyclic Compounds, 2-Ring; D009795:Octanes; D012717:Sesquiterpenes; D013501:Surface-Active Agents; C442489:furanodiene; D012967:Sodium Dodecyl Sulfate; C026728:octane