title: The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors.
abstract: Transcriptional regulation plays a central role in plant hormone signaling. At the core of transcriptional regulation is the Mediator, an evolutionarily conserved, multisubunit complex that serves as a bridge between gene-specific transcription factors and the RNA polymerase machinery to regulate transcription. Here, we report the action mechanisms of the MEDIATOR25 (MED25) subunit of the Arabidopsis thaliana Mediator in regulating jasmonate- and abscisic acid (ABA)-triggered gene transcription. We show that during jasmonate signaling, MED25 physically associates with the basic helix-loop-helix transcription factor MYC2 in promoter regions of MYC2 target genes and exerts a positive effect on MYC2-regulated gene transcription. We also show that MED25 physically associates with the basic Leu zipper transcription factor ABA-INSENSITIVE5 (ABI5) in promoter regions of ABI5 target genes and shows a negative effect on ABI5-regulated gene transcription. Our results reveal that underlying the distinct effects of MED25 on jasmonate and ABA signaling, the interaction mechanisms of MED25 with MYC2 and ABI5 are different. These results highlight that the MED25 subunit of the Arabidopsis Mediator regulates a wide range of signaling pathways through selectively interacting with specific transcription factors.
authors: State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors: Rong
authors: R
authors: Chen
authors: Hongling
authors: H
authors: Jiang
authors: Lin
authors: L
authors: Li
authors: Qingzhe
authors: Q
authors: Zhai
authors: Linlin
authors: L
authors: Qi
authors: Wenkun
authors: W
authors: Zhou
authors: Xiaoqiang
authors: X
authors: Liu
authors: Hongmei
authors: H
authors: Li
authors: Wenguang
authors: W
authors: Zheng
authors: Jiaqiang
authors: J
authors: Sun
authors: Chuanyou
authors: C
authors: Li
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics* / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics* / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D004268:DNA-Binding Proteins; D035264:Flowers / Q000166:cytology / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D018525:Germination; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D054883:Oxylipins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000166:cytology / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000502:physiology; D012639:Seeds / Q000166:cytology / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D013997:Time Factors
chemical_list: C409743:ABI5 protein, Arabidopsis; D029681:Arabidopsis Proteins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D050976:Basic-Leucine Zipper Transcription Factors; D003517:Cyclopentanes; D004268:DNA-Binding Proteins; C473611:MYC2 protein, Arabidopsis; D009687:Nuclear Proteins; D054883:Oxylipins; C475686:PFT1 protein, Arabidopsis; D010937:Plant Growth Regulators; C011006:jasmonic acid; D000040:Abscisic Acid