title: Assessment of the probiotic potential of a dairy product fermented by Propionibacterium freudenreichii in piglets.
abstract: Dairy propionibacteria, including  Propionibacterium freudenreichii , display promising probiotic properties, including immunomodulation. These properties are highly strain-dependent and rarely studied in a fermented dairy product. We screened 10 strains, grown in a newly developed fermented milk ultrafiltrate, for immunomodulatory properties in vitro. The most anti-inflammatory strain,  P. freudenreichii  BIA129, was further tested on piglets.  P. freudenreichii -fermented product improved food intake and growth of piglets. Colonic mucosa explants of treated pigs secreted less interleukin 8 (-25%, P < 0.05) and tumor necrosis factor α (-20%, P < 0.05), either in basal conditions or after a lipopolysaccharide challenge. By contrast, the gut structure, barrier function (measured ex vivo in Ussing chambers), microbial diversity (assessed by 16S rRNA pyrosequencing), and colonic short-chain fatty acid content were unchanged, assuming maintenance of normal intestinal physiology. In conclusion, this work confirms in vivo probiotic properties of dairy propionibacteria-fermented products, which are promising for the prevention or healing of inflammatory bowel diseases.
authors: UMR 1253, Science et Technologie du Lait et de l'Œuf, INRA, F-35042 Rennes, France.
authors: Fabien J
authors: FJ
authors: Cousin
authors: Benoît
authors: B
authors: Foligné
authors: Stéphanie-Marie
authors: SM
authors: Deutsch
authors: Sébastien
authors: S
authors: Massart
authors: Sandrine
authors: S
authors: Parayre
authors: Yves
authors: Y
authors: Le Loir
authors: Gaëlle
authors: G
authors: Boudry
authors: Gwénaël
authors: G
authors: Jan
mesh_terms: D000818:Animals; D003106:Colon / Q000276:immunology / Q000378:metabolism; D004435:Eating; D005260:Female; D005285:Fermentation; D056747:Immunomodulation / Q000502:physiology; D016209:Interleukin-8 / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D008892:Milk / Q000382:microbiology*; D019936:Probiotics; D011424:Propionibacterium / Q000254:growth & development / Q000502:physiology*; D013045:Species Specificity; D034421:Sus scrofa / Q000254:growth & development / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha