title: Fabrication of titanium based MALDI bacterial chips for rapid, sensitive and direct analysis of pathogenic bacteria.
abstract: For the first time, we report the fabrication of a titanium bacterial chip for MALDI-MS produced from a simple, cost effective and rapid heat treatment process. This bacterial chip can be reused many times and is highly versatile. These bacterial chips serve dual roles: (1) They can be applied as MALDI-MS target plates for direct and highly sensitive bacterial analysis. (2) They can be used as bacterial sensors for direct analysis of the captured bacteria using MALDI-MS. The sensitivity of these chips when used as bacterial sensors is <10(3)cfu/mL. The lowest detectable concentration for direct MALDI-MS analysis was found to be 10(4)cfu/mL. The results were further justified by using standard plate counting method combined with Tukey-Kramer statistical analysis and fluorescence imaging followed by image processing for fluorescence quantification using ImageJ software to substantiate the MALDI-MS results.
authors: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan.
authors: Judy
authors: J
authors: Gopal
authors: Nazim
authors: N
authors: Hasan
authors: Hui-Fen
authors: HF
authors: Wu
mesh_terms: D006801:Humans; D056656:Lab-On-A-Chip Devices / Q000191:economics; D011552:Pseudomonas Infections / Q000175:diagnosis; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000191:economics / Q000295:instrumentation*; D013203:Staphylococcal Infections / Q000175:diagnosis; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
chemical_list: D014025:Titanium