title: Evaluation of liver regeneration by modulation with ischemic preconditioning after ischemia and reperfusion and partial hepatectomy.
abstract: OBJECTIVE
  To evaluate liver regeneration modulated by ischemic preconditioning after ischemia, reperfusion and partial hepatectomy.
  
  
  METHODS
  We used 24 Wistar rats of 12 weeks of age, which were randomly assigned into four groups: control (SHAM), hepatectomy (HEP), ischemia and reperfusion (IRG) and Ischemic Preconditioning (SRG). Analyses were made on liver enzymes ALT and AST, assessment of regeneration through the initial and final weight of the liver and the proliferation of hepatocytes by immunohistochemical analysis with Proliferating Cell Nuclear Antigen (PCNA).
  
  
  RESULTS
  In all groups there was liver regeneration, with no statistically significant difference between them. There were significant differences in ALT and AST between groups HEP and SHAM, PRE and GIR, GIR and SHAM and PRE and SHAM (p <0.05). There were also significant differences in the PCNA labeling of the SHAM group as compared to other groups (p <0.05).
  
  
  CONCLUSIONS
  The ischemic preconditioning decreased liver injury, but did not influence the regeneration up to 48 hours.
authors: Medical Sciences, Fluminense Federal University, Niterói-RJ-Brazil.
authors: Laura Sampaio
authors: LS
authors: Salomão
authors: Silvia Barbosa
authors: SB
authors: Young
authors: Maria Aparecida
authors: MA
authors: Galhardo
authors: Leandro Alves
authors: LA
authors: Pereira
authors: Andréa Rodrigues Cordovil
authors: AR
authors: Pires
authors: Gilson Teles
authors: GT
authors: Boaventura
authors: Ana Maria Reis
authors: AM
authors: Ferreira
authors: José Manoel
authors: JM
authors: Martinho
mesh_terms: D000818:Animals; D006498:Hepatectomy / Q000379:methods*; D019194:Ischemic Preconditioning; D008115:Liver Regeneration; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury