title: Diagnostic performance of multiplex cytokine and chemokine assay for tuberculosis.
abstract: Simultaneous detection of multiple biomarkers might lead to improved diagnostic performance for Mycobacterium tuberculosis infection. In this study, we screened soluble biomarkers that had significant differences in patients with active tuberculosis and healthy controls and evaluated the diagnostic performance of the multiplex cytokine/chemokine assay. Overall, 178 patients with active pulmonary tuberculosis, 156 healthy individuals and 35 patients with bacterial pneumonia or lung cancer were evaluated. Among the 16 soluble biomarkers screened by the microbead-based multiplex assay, five cytokines/chemokines including IFN-γ, IP-10, MIG, TNF-α and IL-2 that showed most significant differences between active pulmonary tuberculosis patients and healthy controls were selected for further analysis. When analyzed individually, both IP-10 and MIG had sensitivity and specificity comparable to IFN-γ in detection of active TB. Combined detection of IFN-γ, IP-10 and MIG had significantly improved sensitivity and specificity as compared with individual cytokine and chemokine detection. The responsive levels of IFN-γ, IP-10, MIG, TNF-α and IL-2 were significantly lower in re-treatment pulmonary tuberculosis patients than in new tuberculosis patients. It is concluded that combined IFN-γ, IP-10, MIG multiplex detection had better diagnostic performance for tuberculosis than the individual cytokine/chemokine assays. The re-treatment pulmonary tuberculosis patients had poor responses to ESAT-6/CFP-10 peptides stimulation.
authors: Division of Research, Institute of Tuberculosis, 309 Hospital, 17 Hei Shan Hu Road, Haidian, Beijing 100091, China.
authors: Xinjing
authors: X
authors: Wang
authors: Jing
authors: J
authors: Jiang
authors: Zhihong
authors: Z
authors: Cao
authors: Bingfen
authors: B
authors: Yang
authors: Jinhua
authors: J
authors: Zhang
authors: Xiaoxing
authors: X
authors: Cheng
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis; D000995:Antitubercular Agents / Q000008:administration & dosage; D001426:Bacterial Proteins / Q000032:analysis; D015415:Biomarkers; D018925:Chemokines / Q000032:analysis; D002681:China; D016207:Cytokines / Q000032:analysis; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000032:analysis; D008175:Lung Neoplasms / Q000175:diagnosis / Q000276:immunology*; D008297:Male; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques; D018410:Pneumonia, Bacterial / Q000175:diagnosis / Q000276:immunology*; D040081:Protein Array Analysis; D012680:Sensitivity and Specificity; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D000995:Antitubercular Agents; D001426:Bacterial Proteins; D015415:Biomarkers; D018925:Chemokines; D016207:Cytokines; D007371:Interferon-gamma