title: Expression of hBD-2 induced by 23-valent pneumococcal polysaccharide vaccine, Haemophilus influenzae type b vaccine and split influenza virus vaccine.
abstract: Human β-defensin-2 (hBD-2) is an antimicrobial peptide with high activity and broad spectrum activity. hBD-2 expression may be highly elevated by microorganisms and inflammation. We reported that the majority of common vaccines used, including 23-valent pneumococcal polysaccharide vaccine, Haemophilus influenzae type b vaccine and split influenza virus vaccine, could induce the expression of hBD-2 in epithelial cells. Among them, the 23-valent pneumococcal polysaccharide vaccine was effective at a lower concentration (0.5 µg/ml), while Haemophilus influenzae type b vaccine and split influenza virus vaccine were effective at the concentration of 1 µg/ml. However, bacteriostatic experiments revealed that the split influenza virus vaccine was capable of inducing the highest antimicrobial activity. The medium of the 23-valent pneumococcal polysaccharide vaccine treatment group had a higher antimicrobial activity than the medium of the Haemophilus influenzae type b vaccine treatment group. The transcriptional regulator of hBD-2, that is, the NF-κB subunit, had a high level of activity, while the normal epithelial cells showed barely detectable activity, indicating that these vaccines have potential for clinical application.
authors: Department of Intensive Care Unit, Eastern Hospital, Tongji University, Shanghai 200120, PR China.
authors: Zhenwei
authors: Z
authors: Shen
authors: Han
authors: H
authors: Lei
mesh_terms: D016667:Bacterial Capsules; D001980:Bronchi / Q000166:cytology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D018073:Haemophilus Vaccines / Q000494:pharmacology*; D006801:Humans; D007252:Influenza Vaccines / Q000494:pharmacology*; D016328:NF-kappa B / Q000378:metabolism; D022242:Pneumococcal Vaccines / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*; D023083:beta-Defensins / Q000235:genetics / Q000378:metabolism*
chemical_list: C414006:23-valent pneumococcal capsular polysaccharide vaccine; C106641:DEFB4A protein, human; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D007252:Influenza Vaccines; D016328:NF-kappa B; D022242:Pneumococcal Vaccines; D023083:beta-Defensins