title: The strength of the miR398-Csd2-CCS1 regulon is subject to natural variation in Arabidopsis thaliana.
abstract: miR398 links expression of the three major chloroplast copper proteins, plastocyanin, CCS1 and Csd2, to copper availability. miR398 abundance was stronger plastocyanin-controlled in accessions from cold and continental habitats (Kas-1, Ms-0, WS) than in Cvi-0 and Col-0. Target gene regulation was broken for Csd2 in Cvi-0 upon cold-treatment. Comparison of miR398 levels, target gene regulation as well as Ago1 and miR168 expression demonstrated that the miR398 regulon can be overwritten by accession specific transcriptional regulation in Cvi-0. It is concluded that the escape from the miRNA control of copper homeostasis is linked to adaptation of Cvi-0 to its harsh natural habitat.
authors: Plant Physiology, Freie Universität Berlin, Berlin, Germany.
authors: Ilona
authors: I
authors: Juszczak
authors: Margarete
authors: M
authors: Baier
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D060365:Chloroplast Proteins / Q000235:genetics* / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D017753:Ecosystem; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism; D010970:Plastocyanin / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D018085:Regulon / Q000235:genetics*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000099156:Superoxide Dismutase 2
chemical_list: D029681:Arabidopsis Proteins; D060565:Argonaute Proteins; D002352:Carrier Proteins; D060365:Chloroplast Proteins; D003300:Copper; D018744:DNA, Plant; D035683:MicroRNAs; D018832:Molecular Chaperones; D010970:Plastocyanin; D018749:RNA, Plant; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2; C117457:AGO1 protein, Arabidopsis; C433183:CCH protein, Arabidopsis; C543882:MIRN398 microRNA, Arabidopsis; C543877:PETE2 protein, Arabidopsis; C023548:copper-binding protein