title: [Refinement of taxonomic position of Lactobacillus genus probiotic strains by 16S rDNA and rpoA gene sequencing].
abstract: OBJECTIVE
  Revision of the species identification of collection lactobacilli strains based on 16S rDNA and rpoA gene sequencing.
  
  
  METHODS
  52 lactobacilli cultures that present mostly Gamaleya Research Institute of Epidemiology and Microbiology (GIMC) collection were studied. 16S rDNA gene fragments were amplified by using Lb16a, Lb16b, 16S-midford, 16S-midrev primers. 2 different reverse primers were used for the analysis of rpoA gene depending on lactobacilli species. DNA fragments sequencing was performed with 3130 Genetic Analyzer (Applied Biosystems/Hitachi) with primers used for amplification.
  
  
  RESULTS
  The effectiveness of sequencing of 2 targets for differentiation of species within lactobacilli phylogenetic groups was shown. Species diversity was demonstrated for GIMC lactobacilli strain collection that includes members of 9 species. All the strains marked previously as L. acidophilus were determined to belong to L. helveticus. Strains belonging to recently discovered L. farraginis species that has promising application in agriculture were detected.
  
  
  CONCLUSIONS
  Genetic passports of original strains of 9 species of lactobacilli that are promising for further research.
authors: O L
authors: OL
authors: Voronina
authors: M S
authors: MS
authors: Kunda
authors: V M
authors: VM
authors: Bondarenko
authors: N A
authors: NA
authors: Shabanova
authors: V G
authors: VG
authors: Lunin
mesh_terms: D017931:DNA Primers / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000145:classification / Q000235:genetics*; D007778:Lactobacillus / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit