title: Amplified electrochemiluminescence detection of nucleic acids by hairpin probe-based isothermal amplification.
abstract: Here, we present a straightforward method for isothermal amplified detection of nucleic acids. In this proof-of-concept study, a specific DNA sequence is amplified through hairpin probe-based isothermal strand-displacement polymerization reaction and then detected via a sensitive and commercially available ECL detection platform. Results show that the DNA sequence derived from the Listeria monocytogenes hly gene can be detected down to 10 pM in solution, together with correlation of the detected signal with the initial concentration of target DNA. Moreover, the designed stem-loop structured hairpin probe shows single-base variation differentiating ability. Considering the superior sensitivity and specificity, as well as the simple-to-implement features, the developed assay demonstrates a great potential of becoming a first-line tool for quantitative analysis of nucleic acids for biomedical research.
authors: MOE key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
authors: Xiaoming
authors: X
authors: Zhou
authors: Da
authors: D
authors: Xing
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D055664:Electrochemical Techniques; D008089:Listeria monocytogenes / Q000235:genetics*; D049449:Luminescence; D021141:Nucleic Acid Amplification Techniques; D009696:Nucleic Acids / Q000032:analysis*; D015345:Oligonucleotide Probes; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial; D009696:Nucleic Acids; D015345:Oligonucleotide Probes