title: Membrane proteins of human fetal primitive nucleated red blood cells.
abstract: In humans, primitive fetal nucleated red blood cells (FNRBCs) are thought to be as vital for embryonic life as their counterpart, adult red blood cells (adult RBCs) are in later-gestation fetuses and adults. Unlike adult RBCs, the identity and functions of FNRBC proteins are poorly understood owing to a scarcity of FNRBCs for proteomic investigations. The study aimed to investigate membrane proteins of this unique cell type. We present here, the first report on the membrane proteome of human primitive FNRBCs investigated by two-dimensional liquid chromatography coupled with mass-spectrometry (2D-LCMS/MS) and bioinformatics analysis. A total of 273 proteins were identified, of which 133 (48.7%) were membrane proteins. We compared our data with membrane proteins of adult RBCs to identify common, and unique, surface membrane proteins. Twelve plasma membrane proteins with transmembrane domains and eight proteins with transmembrane domains but without known sub-cellular location were identified as unique-to-FNRBCs. Except for the transferrin receptor, all other 19 unique-to-FNRBC membrane proteins have never been described in RBCs. Reverse-transcriptase PCR (RT-PCR) and immunocytochemistry validated the 2D-LCMS/MS data. Our findings provide potential surface antigens for separation of primitive FNRBCs from maternal blood for noninvasive prenatal diagnosis, and to understand the biology of these rare cells.
authors: Department of Obstetrics and Gynecology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore.
authors: Sukumar
authors: S
authors: Ponnusamy
authors: Huoming
authors: H
authors: Zhang
authors: Priya
authors: P
authors: Kadam
authors: Qingsong
authors: Q
authors: Lin
authors: Teck Kwang
authors: TK
authors: Lim
authors: Jaspal Singh
authors: JS
authors: Sandhu
authors: Narasimhan
authors: N
authors: Kothandaraman
authors: Aniza Puteri
authors: AP
authors: Mahyuddin
authors: Arijit
authors: A
authors: Biswas
authors: Annapoorna
authors: A
authors: Venkat
authors: Choy-Leong
authors: CL
authors: Hew
authors: Shashikant B
authors: SB
authors: Joshi
authors: Maxey Ching Ming
authors: MC
authors: Chung
authors: Mahesh
authors: M
authors: Choolani
mesh_terms: D004900:Erythroblasts / Q000737:chemistry*; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005333:Fetus; D006801:Humans; D008565:Membrane Proteins / Q000097:blood*; D011247:Pregnancy; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008565:Membrane Proteins