title: Glycohistochemical characterization of histologically normal nasal mucosa and enzootic nasal tumor of sheep.
abstract: OBJECTIVE
  To determine glycohistochemical characteristics of enzootic nasal tumors (ENTs) of sheep, compare results for ENT with those of histologically normal nasal mucosa of sheep, and identify the histologic origin of ENT.
  
  
  METHODS
  ENT and nasal mucosa samples obtained from cadavers of 5 adult Lacaune sheep with ENT and 5 Lacaune sheep unaffected by ENT, respectively.
  
  
  METHODS
  Samples of ENT and nasal mucosa were collected from cadavers of sheep and sectioned. Conventional and lectin histochemical analyses were used to identify glycoconjugates in tissue sections on the basis of their principal chemical groups and principal terminal or internal oligosaccharidic glucidic residues, respectively.
  
  
  RESULTS
  ENTs had papillary and tubular portions. Cells in the papillary portion of ENTs had secretion and surface glycoconjugates, which included sulfated glycosaminoglycans and neutral and sialilated glycoproteins. Cells in the tubular portion of ENTs had surface glycoconjugates, which included neutral and sialilated glycoproteins. Both portions of ENTs had C(4)-acetylated sialoderivatives that were not detected in sections of histologically normal nasal mucosa.
  
  
  CONCLUSIONS
  The papillary portion of ENTs in sheep may originate from respiratory glands and goblet cells. The tubular portion of ENTs in sheep may originate from olfactory glands. Presence of C(4)-acetylated sialoderivatives in cells of ENTs could confer resistance against pathogens to those cells.
authors: School of Environmental Sciences, University of Camerino, Macerata, Italy.
authors: Paola
authors: P
authors: Scocco
authors: Elvio
authors: E
authors: Lepri
authors: Francesca
authors: F
authors: Mercati
authors: Giovanni
authors: G
authors: Vitellozzi
authors: Luca
authors: L
authors: Mechelli
authors: Piero
authors: P
authors: Ceccarelli
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D000818:Animals; D030121:Betaretrovirus / Q000502:physiology; D006001:Glycoconjugates / Q000378:metabolism*; D006651:Histocytochemistry / Q000662:veterinary; D037102:Lectins / Q000737:chemistry; D009297:Nasal Mucosa / Q000166:cytology / Q000473:pathology / Q000821:virology; D009669:Nose Neoplasms / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D012192:Retroviridae Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D012756:Sheep; D012757:Sheep Diseases / Q000473:pathology* / Q000821:virology; D014412:Tumor Virus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology
chemical_list: D006001:Glycoconjugates; D037102:Lectins