title: Toll-like receptor 3 polymorphism and its association with hepatitis B virus infection in Saudi Arabian patients.
abstract: Hepatitis B virus (HBV) is the major causative agent of chronic liver complications including cirrhosis and hepatocellular carcinoma (HCC). Individuals infected with HBV show a wide spectrum of disease manifestations ranging from asymptomatic carriers to HCC. TLR3 is part of the innate immune system that recognizes double-stranded RNA (dsRNA) and provides early immune response to exogenous antigens. The genetic polymorphisms such as single nucleotide polymorphisms (SNPs) in the TLR3 could be considered as factors for the susceptibility to viral pathogens including HBV. Due to lack of knowledge on the role of TLR3 polymorphisms in HBV infection, this study investigated the distribution of nine SNPs in the TLR3 gene and its association with Saudi Arabian patients infected with HBV. A total of 707 patients and 600 uninfected controls were examined for different parameters including the nine SNPs (rs5743311, rs5743312, rs1879026, rs5743313, rs5743314, rs5743315, rs111611328, rs78726532 and a newly identified SNP located at position 184322913 of chr4). The association analysis confirmed that only one SNP, rs1879026 (G/T), showed a significant difference (P = 0.0480; OR = 0.809, 95% CI = 0.655-0.999) in the distribution between HBV carriers and uninfected controls. While, the rest of the SNPs showed no significant association with regards to HBV infection or in the progression to cirrhosis of the liver and HCC. Furthermore, haplotype analysis revealed that one haplotype GCGA (rs1879026, rs5743313, rs5743314, and rs5743315, respectively), was associated significantly with HBV infection in this population. These findings indicate that genetic variations in the TLR3 gene could affect the outcome of HBV infection among Saudis.
authors: Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia.
authors: Ahmed
authors: A
authors: Al-Qahtani
authors: Mohammed
authors: M
authors: Al-Ahdal
authors: Ayman
authors: A
authors: Abdo
authors: Faisal
authors: F
authors: Sanai
authors: Mashael
authors: M
authors: Al-Anazi
authors: Nisreen
authors: N
authors: Khalaf
authors: Nisha A
authors: NA
authors: Viswan
authors: Hamad
authors: H
authors: Al-Ashgar
authors: Hind
authors: H
authors: Al-Humaidan
authors: Riham
authors: R
authors: Al-Suwayeh
authors: Zahid
authors: Z
authors: Hussain
authors: Saud
authors: S
authors: Alarifi
authors: Majid
authors: M
authors: Al-Okail
authors: Fahad N
authors: FN
authors: Almajhdi
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006239:Haplotypes; D006515:Hepatitis B virus; D019694:Hepatitis B, Chronic / Q000097:blood / Q000235:genetics* / Q000821:virology; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012529:Saudi Arabia; D017422:Sequence Analysis, DNA; D051196:Toll-Like Receptor 3 / Q000235:genetics*; D019562:Viral Load
chemical_list: C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3