title: Analysis of renal cell carcinoma as a first step for developing mass spectrometry-based diagnostics.
abstract: Immediate diagnosis of human specimen is an essential prerequisites in medical routines. This study aimed to establish a novel cancer diagnostics system based on probe electrospray ionization-mass spectrometry (PESI-MS) combined with statistical data processing. PESI-MS uses a very fine acupuncture needle as a probe for sampling as well as for ionization. To demonstrate the applicability of PESI-MS for cancer diagnosis, we analyzed nine cases of clear cell renal cell carcinoma (ccRCC) by PESI-MS and processed the data by principal components analysis (PCA). Our system successfully delineated the differences in lipid composition between non-cancerous and cancerous regions. In this case, triacylglycerol (TAG) was reproducibly detected in the cancerous tissue of nine different individuals, the result being consistent with well-known profiles of ccRCC. Moreover, this system enabled us to detect the boundaries of cancerous regions based on the expression of TAG. These results strongly suggest that PESI-MS will be applicable to cancer diagnosis, especially when the number of data is augmented.
authors: Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Japan.
authors: Kentaro
authors: K
authors: Yoshimura
authors: Lee Chuin
authors: LC
authors: Chen
authors: Mridul Kanti
authors: MK
authors: Mandal
authors: Tadao
authors: T
authors: Nakazawa
authors: Zhan
authors: Z
authors: Yu
authors: Takahito
authors: T
authors: Uchiyama
authors: Hirokazu
authors: H
authors: Hori
authors: Kunio
authors: K
authors: Tanabe
authors: Takeo
authors: T
authors: Kubota
authors: Hideki
authors: H
authors: Fujii
authors: Ryohei
authors: R
authors: Katoh
authors: Kenzo
authors: K
authors: Hiraoka
authors: Sen
authors: S
authors: Takeda
mesh_terms: D002292:Carcinoma, Renal Cell / Q000737:chemistry* / Q000175:diagnosis; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007668:Kidney / Q000737:chemistry*; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000175:diagnosis; D057054:Molecular Imaging / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014280:Triglycerides / Q000737:chemistry
chemical_list: D010743:Phospholipids; D014280:Triglycerides