title: Simultaneous determination of three flavonoid C-glycosides in mice biosamples by HPLC-ESI-MS method after oral administration of Abrus mollis extract and its application to biodistribution studies.
abstract: A simple HPLC-ESI-MS method was developed for the determination of vicenin-2 (1), isoschaftoside (2), and schaftoside (3) of Abrus mollis extract in mice plasma and tissues (heart, liver, spleen, lungs, and kidneys). The separation was achieved by HPLC on a Shim-Pack CLC-ODS column with a mobile phase composed of 0.1% formic acid (mobile phase A, 72%) and methanol-isopropanol (9:1) (mobile phase B, 28%). The electrospray source of the MS was operated in the selective ion monitoring (SIM) mode at m/z 593 ([M-H]⁻) for 1, and 563 ([M-H]⁻) for 2 and 3, respectively. The limit of detection (LOD) of 1-3 was in the range of 8.5-12.6 ng/mL for plasma, and 32.5-49.4 ng/g for tissue tested. The limit of quantification (LOQ) was found to be 25 ng/mL for plasma, and 100 ng/g for tissue. The calibration curves were linear in all matrices (r² > 0.994) in the concentration range of 25-500 ng/mL in plasma or 100-1250 ng/g in tissue, respectively. Intra-day and inter-day precision studies demonstrate that the method is precise with coefficients of variation intra-day and inter-day below 5.9 and 7.4% for all the samples, respectively. The recoveries of three flavonoid C-glycosides ranged from 95.3 to 106.4% for plasma, and 92.6 to 107.3% for tissues. Following oral administration of A. mollis extract to mice at a dose of 72 mg/kg, the concentrations of 1-3 in plasma and tissues were quantifiable in bio-samples collected up to 180 min. The method described is suitable for studies on the distribution of three flavonoid C-glycosides of A. mollis extract in plasma and different tissues of mice.
authors: State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, China. btwanghao@yahoo.com.cn
authors: Hao
authors: H
authors: Wang
authors: Zhenzhou
authors: Z
authors: Jiang
authors: Huibing
authors: H
authors: Du
authors: Chunyi
authors: C
authors: Liang
authors: Yuanchao
authors: Y
authors: Wang
authors: Mohan
authors: M
authors: Zhang
authors: Luyong
authors: L
authors: Zhang
authors: Wencai
authors: W
authors: Ye
authors: Ping
authors: P
authors: Li
mesh_terms: D029912:Abrus / Q000737:chemistry*; D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D006027:Glycosides / Q000032:analysis / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D057230:Limit of Detection; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D005419:Flavonoids; D006027:Glycosides; D010936:Plant Extracts