title: Triterpene acids from Euscaphis japonica and assessment of their cytotoxic and anti-NO activities.
abstract: Six new triterpenoids, euscaphic acids G-L (1-6), along with nine known triterpene acids, and two known lignans were isolated from the ethanolic extract of the twigs of Euscaphis japonica. This is the first report concerning 1α,3β-dihydroxy-12-oleanen-28-oic acid isolated from a natural source. The structures of the new compounds were established by spectroscopic analysis. The cytotoxic and anti-NO production activities for the isolates are also evaluated and discussed; compound 1, hederagenin (11), and arjunic acid (12) showed significant cytotoxicity against NCI-H460 cells, HT-29 cells, and CEM cells (IC₅₀ = 1.64 ± 0.87, 2.11 ± 1.54, 1.73 ± 0.64 µM, respectively). Some of the isolated triterpenoids showed marginal inhibitions on NO production induced by LPS.
authors: National Research Institute of Chinese Medicine, Taipei, Taiwan.
authors: Li-Jie
authors: LJ
authors: Zhang
authors: Jing-Jy
authors: JJ
authors: Cheng
authors: Chia-Ching
authors: CC
authors: Liao
authors: Hui-Ling
authors: HL
authors: Cheng
authors: Hung-Tse
authors: HT
authors: Huang
authors: Li-Ming Yang
authors: LM
authors: Kuo
authors: Yao-Haur
authors: YH
authors: Kuo
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D020128:Inhibitory Concentration 50; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D019684:Magnoliopsida / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000009:adverse effects; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017705:Lignans; D010936:Plant Extracts; D011134:Polysaccharides; D014315:Triterpenes; C441431:arjunic acid; D009569:Nitric Oxide; C007695:euscaphic acid; D009828:Oleanolic Acid; C025763:hederagenin