title: Characterization of catechol 2,3-dioxygenase from Planococcus sp. strain S5 induced by high phenol concentration.
abstract: This study aimed at characterization of a new catechol 2,3-dioxygenase isolated from a Gram-positive bacterium able to utilize phenol as the sole carbon and energy source. Planococcus sp. strain S5 grown on 1 or 2 mM phenol showed activity of both a catechol 1,2- and catechol 2,3-dioxygenase while at a higher concentrations of phenol only catechol 2,3-dioxygenase activity was observed. The enzyme was optimally active at 60°C and pH 8.0. Kinetic studies showed that the K(m) and V(max) of the enzyme were 42.70 µM and 329.96 mU, respectively. The catechol 2,3-dioxygenase showed the following relative meta-cleavage activities for various catechols tested: catechol (100%), 3-methylcatechol (13.67%), 4-methylcatechol (106.33%) and 4-chlorocatechol (203.80%). The high reactivity of this enzyme towards 4-chlorocatechol is different from that observed for other catechol 2,3-dioxygenases. Nucleotide sequencing and homology search revealed that the gene encoding the S5 catechol 2,3-dioxygenase shared the greatest homology with the known genes encoding isoenzymes from Gram-negative Pseudomonas strains.
authors: Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Poland.
authors: Katarzyna
authors: K
authors: Hupert-Kocurek
authors: Urszula
authors: U
authors: Guzik
authors: Danuta
authors: D
authors: Wojcieszyńska
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism; D004789:Enzyme Activation; D004795:Enzyme Stability; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D019800:Phenol / Q000378:metabolism*; D058642:Planococcus Bacteria / Q000201:enzymology* / Q000235:genetics; D016415:Sequence Alignment; D017385:Sequence Homology; D013045:Species Specificity; D013379:Substrate Specificity; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D002396:Catechols; D003470:Culture Media; C031136:3-methylcatechol; C018599:4-methylcatechol; D019800:Phenol; D050557:Catechol 2,3-Dioxygenase; C038861:4-chlorocatechol