title: SARA is dispensable for functional TGF-β signaling.
abstract: Smad anchor for receptor activation (SARA or ZFYVE9) has been proposed to mediate transforming growth factor β (TGF-β) signaling by direct interaction with the non-activated Smad proteins and the TGF-β receptors; however, these findings are controversial. We demonstrate no correlation between SARA expression and the levels of TGF-β-induced phosphorylation of Smads in various B-cell lymphomas. Moreover, knockdown of SARA in HeLa cells did not interfere with TGF-β-induced Smad activation, Smad nuclear translocation, or induction of TGF-β target genes. Various R-Smads and TGF-β receptors did not co-immunoprecipitate with SARA. Collectively, our results demonstrate that SARA is dispensable for functional TGF-β-mediated signaling.
authors: Department of Immunology, Institute for Cancer Research, Oslo University Hospital HF, Montebello, Oslo, Norway.
authors: Maren
authors: M
authors: Bakkebø
authors: Kanutte
authors: K
authors: Huse
authors: Vera I
authors: VI
authors: Hilden
authors: Lise
authors: L
authors: Forfang
authors: June H
authors: JH
authors: Myklebust
authors: Erlend B
authors: EB
authors: Smeland
authors: Morten P
authors: MP
authors: Oksvold
mesh_terms: D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D016393:Lymphoma, B-Cell / Q000378:metabolism; D010766:Phosphorylation; D034741:RNA, Small Interfering / Q000235:genetics; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D051896:Smad Proteins, Receptor-Regulated / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D034741:RNA, Small Interfering; D018125:Receptors, Transforming Growth Factor beta; D011994:Recombinant Proteins; D051896:Smad Proteins, Receptor-Regulated; D053773:Transforming Growth Factor beta1; C116420:ZFYVE16 protein, human; D012697:Serine Endopeptidases