title: [Time-effect and dose-effect relationships study on the effect of tonifying kidney and activating blood formulas contained serum on MSCs proliferation and osteogenic differentiation].
abstract: OBJECTIVE
  To screen the optimal cell proliferation and osteogenic differentiation concentration of SD rats' Mesenchymal stem cells (MSCs) affected by compound Bushenhuoxue (CB) contained serum.
  
  
  METHODS
  30 SD female rats, aged 10 months, were randomly divided into low-dose (11.6 g/kg), medium dose (34.8 g/kg) and high dose CB group (104.4 g/kg), 10 rats in each group (qd, 12 d). Rats blood of three groups were taken respectively in 0.5, 1.0, 1.5, 2.0, 3.0 h after the last intragastric infusion, blood serum was separated, and different concentrations of CB contained serum were given to MSCs form SD female rats aged 3 months. MTT method was used to determine the effect of CB on MSCs proliferation. Alkaline phosphatase (ALP) was detected to determine the osteogenic differentiation of MSCs from each group.
  
  
  RESULTS
  The 25% concentration of CB contained serum collected from SD rats one hour after intragastric infusion with low dose CB was proved to be the most effective concentration on the proliferation of MSCs; The 25%-30% concentration of CB contained serum collected from SD rats one hour after intragastric infusion with high dose CB was proved to be the most effective concentration on the differentiations of MSCs into osteoblast.
  
  
  CONCLUSIONS
  CB contained serum could promote the proliferation and osteogenic differentiation of MSCs.
authors: Pharmacy College, Jinan University, Guangzhou 510632, China. doctormonkey@126.com
authors: Li
authors: L
authors: Yang
authors: Xiao-feng
authors: XF
authors: Zhu
authors: Rong-hua
authors: RH
authors: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum / Q000737:chemistry; D013997:Time Factors
chemical_list: D003470:Culture Media; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D000469:Alkaline Phosphatase