title: VpWRKY3, a biotic and abiotic stress-related transcription factor from the Chinese wild Vitis pseudoreticulata.
abstract: Chinese wild grapevine Vitis pseudoreticulata accession 'Baihe-35-1' is identified as the precious resource with multiple resistances to pathogens. A directional cDNA library was constructed from the young leaves inoculated with Erysiphe necator. A total of 3,500 clones were sequenced, yielding 1,727 unigenes. Among them, 762 unigenes were annotated and classified into three classes, respectively, using Gene Ontology, including 22 ESTs related to transcription regulator activity. A novel WRKY transcription factor was isolated from the library, and designated as VpWRKY3 (GenBank Accession No. JF500755). The full-length cDNA is 1,280 bp, encoding a WRKY protein of 320 amino acids. VpWRKY3 is localized to nucleus and functions as a transcriptional activator. QRT-PCR analysis showed that the VpWRKY3 specifically accumulated in response to pathogen, salicylic acid, ethylene and drought stress. Overexpression of VpWRKY3 in tobacco increased the resistance to Ralstonia solanacearum, indicating that VpWRKY3 participates in defense response. Furthermore, VpWRKY3 is also involved in abscisic acid signal pathway and salt stress. This experiment provided an important basis for understanding the defense mechanisms mediated by WRKY genes in China wild grapevine. Generation of the EST collection from the cDNA library provided valuable information for the grapevine breeding.                 Key message We constructed a cDNA library from Chinese wild grapevine leaves inoculated with powdery mildew. VpWRKY3 was isolated and demonstrated that it was involved in biotic and abiotic stress responses.
authors: College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People's Republic of China.
authors: Ziguo
authors: Z
authors: Zhu
authors: Jiangli
authors: J
authors: Shi
authors: Jiangling
authors: J
authors: Cao
authors: Mingyang
authors: M
authors: He
authors: Yuejin
authors: Y
authors: Wang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001203:Ascomycota / Q000502:physiology*; D001483:Base Sequence; D060467:Disease Resistance / Q000235:genetics*; D055864:Droughts; D005030:Ethylenes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D043368:Ralstonia solanacearum / Q000502:physiology*; D020156:Salicylic Acid / Q000494:pharmacology; D055049:Salt Tolerance; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000382:microbiology / Q000502:physiology; D016415:Sequence Alignment; D015398:Signal Transduction; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D027843:Vitis / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology / Q000502:physiology
chemical_list: D005030:Ethylenes; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid; C036216:ethylene; D020156:Salicylic Acid