title: Stability studies of common biochemical analytes in serum separator tubes with or without gel barrier subjected to various storage conditions.
abstract: BACKGROUND
  The collected and shipped blood samples are exposed to a various extra-analytical factors prior to analysis. The aim of the study was to determine the stability of analytes in serum gel tubes and plain tubes exposed to a range of storage temperatures and times after centrifugation.
  
  
  METHODS
  Fifteen healthy volunteers were recruited and venous blood was collected into four tubes, two with and two without gel separator. Analyzing the baseline samples in 30 min, all were stored at 4 degrees C or 24 degrees C for 6, 12, 18, 24, 30, 36, 48 and 72 hours and 1 week. Sixteen biochemical anaytes were measured on each sample. Variations remained under the desirable bias considered as clinically insignificant.
  
  
  RESULTS
  On day three, most analytes remained stable including albumin, protein, creatinine, cholesterol, triglycerides, gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), alanine aminotransferase (ALT), creatine kinase (CK), lactate dehydrogenase (LD) regardless of tube types. Glucose concentration decreased markedly (P = 0.001) beginning from the first hours of storage in plain serum. The stability maximized for the analytes including glucose, total bilirubin, urea nitrogen (BUN), uric acid stored at 4 degrees C in gel tubes. Aspartate aminotransferase (AST) activity increased significantly (P = 0.002) up to 48-h, however bias was not significant clinically. High density lipoprotein (HDL) concentration was stable in gel tubes at 24 degrees C, in plain tubes at 4 degrees C stored up to 36-h.
  
  
  CONCLUSIONS
  Serum gel or non-gel tubes might be used interchangeably for 11 analytes chilled or at 24 degrees C, whereas some restrictions must be applied for glucose, AST, BUN, HDL, and uric acid.
authors: Ataturk Training and Research Hospital, Department of Biochemistry and Clinical Biochemistry, Izmir, Turkey. sdcuhadar@yahoo.com
authors: Serap
authors: S
authors: Cuhadar
authors: Ayşenur
authors: A
authors: Atay
authors: Mehmet
authors: M
authors: Koseoglu
authors: Ahmet
authors: A
authors: Dirican
authors: Aysel
authors: A
authors: Hur
mesh_terms: D000328:Adult; D001671:Biochemistry / Q000379:methods*; D001800:Blood Specimen Collection / Q000295:instrumentation* / Q000379:methods*; D002498:Centrifugation; D005260:Female; D005782:Gels; D006801:Humans; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D005782:Gels