title: Finished bacterial genomes from shotgun sequence data.
abstract: Exceptionally accurate genome reference sequences have proven to be of great value to microbial researchers. Thus, to date, about 1800 bacterial genome assemblies have been "finished" at great expense with the aid of manual laboratory and computational processes that typically iterate over a period of months or even years. By applying a new laboratory design and new assembly algorithm to 16 samples, we demonstrate that assemblies exceeding finished quality can be obtained from whole-genome shotgun data and automated computation. Cost and time requirements are thus dramatically reduced.
authors: Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
authors: Filipe J
authors: FJ
authors: Ribeiro
authors: Dariusz
authors: D
authors: Przybylski
authors: Shuangye
authors: S
authors: Yin
authors: Ted
authors: T
authors: Sharpe
authors: Sante
authors: S
authors: Gnerre
authors: Amr
authors: A
authors: Abouelleil
authors: Aaron M
authors: AM
authors: Berlin
authors: Anna
authors: A
authors: Montmayeur
authors: Terrance P
authors: TP
authors: Shea
authors: Bruce J
authors: BJ
authors: Walker
authors: Sarah K
authors: SK
authors: Young
authors: Carsten
authors: C
authors: Russ
authors: Chad
authors: C
authors: Nusbaum
authors: Iain
authors: I
authors: MacCallum
authors: David B
authors: DB
authors: Jaffe
mesh_terms: D000465:Algorithms; D001419:Bacteria / Q000235:genetics*; D016680:Genome, Bacterial; D015698:Genomic Library; D017422:Sequence Analysis, DNA / Q000379:methods*