title: Comparison of different non-invasive methods for detection of allergic asthma.
abstract: Non-invasive methods to assess inflammation of lower airways are induced sputum (IS), exhaled nitric oxide (eNO), and exhaled breath condensate (EBC). Here we focused on the assessment of airway inflammation with a panel of non-invasive methods in health care workers (HCWs) with suspected latex allergy with and without current allergic respiratory symptoms about 10 years after the latex ban in German health care facilities. Seventy-seven non-smoking subjects were examined by skin prick test and specific IgE measurements, eNO, IS, and EBC. Sensitivity, specificity, and positive and negative predicted values for relevant biomarkers were calculated using current asthma symptoms as the gold standard. Twenty-nine subjects (38%) reported ongoing asthmatic symptoms (AS). In these subjects the EBC concentrations of nitrogen oxides (NO(x); p=0.027) and leukotriene B(4) (p=0.025) were significantly higher than in subjects without AS. In addition, in the subjects with AS the numbers of eosinophils (p=0.015) and the concentrations of IL-5 (p= 0.021) in IS samples were significantly higher than in the subjects without AS. A good correlation between several inflammatory markers in IS was detected. The maximum Youden Index was reached for IS total eosinophils ≥3.5·10(4) with a test efficiency of 0.72. In conclusion, non-invasive inflammatory monitoring with EBC and IS may assist the diagnosis of allergic asthma. Self-reported current asthmatic symptoms were reflected by eosinophilic inflammation and the best parameter to support the asthma diagnosis is a total number of eosinophils ≥3.5·10(4) in IS.
authors: Institute of the Ruhr-University, Ruhr-University Bochum, Bochum, Germany. raulf@ipa-dguv.de
authors: M
authors: M
authors: Raulf-Heimsoth
authors: V
authors: V
authors: van Kampen
authors: E
authors: E
authors: Heinze
authors: S
authors: S
authors: Bernard
authors: G
authors: G
authors: Borowitzki
authors: S
authors: S
authors: Freundt
authors: K
authors: K
authors: Sucker
authors: C
authors: C
authors: Eliakopoulos
authors: A
authors: A
authors: Nienhaus
authors: T
authors: T
authors: Brüning
authors: R
authors: R
authors: Merget
mesh_terms: D000328:Adult; D001249:Asthma / Q000175:diagnosis*; D001944:Breath Tests; D005260:Female; D006282:Health Personnel; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007975:Leukotriene B4 / Q000032:analysis; D008297:Male; D009569:Nitric Oxide / Q000032:analysis; D012882:Skin Tests
chemical_list: D007975:Leukotriene B4; D009569:Nitric Oxide; D007073:Immunoglobulin E