title: Cytotoxicity of calcium enriched mixture cement compared with mineral trioxide aggregate and intermediate restorative material.
abstract: Calcium enriched mixture (CEM) cement has been recently invented by the last author. It is composed of calcium oxide, calcium phosphate, calcium silicate and calcium sulphate; however, it has a different chemical composition to mineral trioxide aggregate (MTA). The purpose of this ex vivo study was to investigate the cytotoxicity of CEM cement, and compare it with intermediate restorative material (IRM) and MTA. The materials were tested in fresh and set states on L929 fibroblasts to assess their cytotoxicity. The cell viability responses were evaluated with methyl-tetrazolium bromide assay and Elisa reader at 1, 24 and 168 h (7 days). The tested materials were eluted with L929 culture medium according to international standard organisation 109935 standard. Distilled water and culture medium served as positive and negative controls, respectively. Differences in cytotoxicity were evaluated by one-way anova and t-tests. The cytotoxicity of the materials was statistically different at the three time intervals (P < 0.01). The lowest cytotoxic values recorded were expressed by MTA subgroups followed by CEM cement; IRM subgroups were the most cytotoxic root-end/dental material (P < 0.001). CEM cement and MTA are reasonable alternatives to IRM because of lower cytotoxicity. CEM cement also has good biocompatibility as well as lower estimated cost to MTA and seems to be a promising dental material.
authors: Iranian Center for Endodontic research, Iran Dental Research Center, Dental School, Shahid Beheshti Medical University, Tehran, Iran.
authors: Mohammad A
authors: MA
authors: Mozayeni
authors: Amin S
authors: AS
authors: Milani
authors: Laleh A
authors: LA
authors: Marvasti
authors: Saeed
authors: S
authors: Asgary
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017610:Calcium Compounds / Q000737:chemistry / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival; D003738:Dental Cements / Q000737:chemistry / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D008422:Materials Testing; D051379:Mice; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000633:toxicity*
chemical_list: D017610:Calcium Compounds; D003738:Dental Cements; D012387:Root Canal Filling Materials