title: Genotoxic effects of the herbicide Roundup(®) in the fish Corydoras paleatus (Jenyns 1842) after short-term, environmentally low concentration exposure.
abstract: The glyphosate-based herbicide, Roundup(®), is one of the most used pesticides worldwide. In concert with the advent of transgenic crops resistant to glyphosate, the use of this pesticide has led to an increase in agricultural yields. The objective of this study was to evaluate the genotoxic effect that the herbicide Roundup(®) (at a concentration of 6.67 μg/L, corresponding to 3.20 μg/L glyphosate) can have on the fish Corydoras paleatus. Treatment groups were exposed for 3, 6, and 9 days, and effects were analyzed using the piscine micronucleus test (PMT) and comet assay. A group subjected to filtered water only was used as a negative control. The PMT did not show differences between the control and exposed groups for any of the treatment times. In contrast, the comet assay showed a high rate of DNA damage in group exposed to Roundup(®) for all treatment times, both for blood and hepatic cells. We conclude that for the low concentration used in this research, the herbicide shows potential genotoxic effects. Future research will be important in evaluating the effects of this substance, whose presence in the environment is ever-increasing.
authors: Programa de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Universidade Estadual de Maringá, Av Colombo, 5790 Bloco G-90, sala 16, Jardim Universitário, 87020-900 Maringá, Paraná, Brazil. nediaghisi@gmail.com
authors: Nédia
authors: N
authors: de Castilhos Ghisi
authors: Marta Margarete
authors: MM
authors: Cestari
mesh_terms: D000818:Animals; D001681:Biological Assay; D002397:Catfishes; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005998:Glycine / Q000031:analogs & derivatives* / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D000097797:Glyphosate
chemical_list: D005998:Glycine; D006540:Herbicides; D009153:Mutagens; D014874:Water Pollutants, Chemical; D000097797:Glyphosate