title: Interstrand disulfide crosslinking of DNA bases supports a double nucleotide unpairing mechanism for flap endonucleases.
abstract: Flap endonucleases (FENs) are proposed to select their target phosphate diester by unpairing the two terminal nucleotides of duplex. Interstrand disulfide crosslinks, introduced by oxidation of thiouracil and thioguanine bases, abolished the specificity of human FEN1 for hydrolysis one nucleotide into the 5'-duplex.
authors: Centre for Chemical Biology, Department of Chemistry, Krebs Institute, University of Sheffield, Sheffield, S3 7HF, UK.
authors: Amanda
authors: A
authors: Beddows
authors: Nikesh
authors: N
authors: Patel
authors: L David
authors: LD
authors: Finger
authors: John M
authors: JM
authors: Atack
authors: David M
authors: DM
authors: Williams
authors: Jane A
authors: JA
authors: Grasby
mesh_terms: D004247:DNA / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry; D045585:Flap Endonucleases / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D013866:Thioguanine / Q000737:chemistry / Q000378:metabolism*; D013889:Thiouracil / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004220:Disulfides; D013889:Thiouracil; D004247:DNA; D045585:Flap Endonucleases; C468317:FEN1 protein, human; D013866:Thioguanine