title: H5N1 virus causes significant perturbations in host proteome very early in influenza virus-infected primary human monocyte-derived macrophages.
abstract: H5N1 influenza viruses, which cause disease in humans, have unusually high pathogenicity. The temporal response of primary human monocyte-derived macrophages infected with highly pathogenic H5N1 and seasonal H1N1 influenza viruses was evaluated using mass spectrometry-based quantitative proteomic profiling. This was done in order to demonstrate significant perturbation of the host proteome upon viral infection, as early as 1 hour after infection. This early host response distinguished H5N1 infection from H1N1 infection, the latter inducing less of a response. The most pronounced effect was observed on the translational machinery, suggesting that H5N1 might gain advantage in replication by using the cell protein synthesis machinery early in the infection.
authors: Centre of Influenza Research & School of Public Health, University of Hong Kong.
authors: Chung Yan
authors: CY
authors: Cheung
authors: Eric Y
authors: EY
authors: Chan
authors: Alexei
authors: A
authors: Krasnoselsky
authors: David
authors: D
authors: Purdy
authors: Arti T
authors: AT
authors: Navare
authors: Janine T
authors: JT
authors: Bryan
authors: Carolina K L
authors: CK
authors: Leung
authors: Kenrie P Y
authors: KP
authors: Hui
authors: Joseph Sriyal Malik
authors: JS
authors: Peiris
authors: Michael G
authors: MG
authors: Katze
mesh_terms: D054884:Host-Pathogen Interactions; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity / Q000502:physiology*; D007251:Influenza, Human / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000821:virology*; D025341:Principal Component Analysis; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry