title: Effect of an adipose-derived stem cell and nerve growth factor-incorporated hydrogel on recovery of erectile function in a rat model of cavernous nerve injury.
abstract: Postprostatectomy erectile dysfunction (ED) is the major problem for patients with clinically localized prostate cancer. Recently, gene and stem cell-based therapy of the corpus cavernosum has been attempted for postprostatectomy ED, but those therapies are limited by rapid blood flow and disruption of the normal architecture of the corpus cavernosum. In this study, we attempted to regenerate the damaged cavernous nerve (CN), which is the main cause of ED. We investigated the effectiveness of human adipose-derived stem cell (hADSC) and nerve growth factor-incorporated hyaluronic acid-based hydrogel (NGF-hydrogel) application on the CN in a rat model of bilateral cavernous nerve crush injury. Four weeks after the operation, erectile function was assessed by detecting the intracavernous pressure (ICP)/arterial pressure level by CN electrostimulation. The ICP was significantly increased by application of hADSC with NGF-hydrogel compared to the other experimental groups. CN and penile tissue were collected for histological examination. PKH-26 labeled hADSC colocalized with beta III tubulin were shown in CN tissue sections. hADSC/NGF-hydrogel treatment prevented smooth muscle atrophy in the corpus cavernosum. In addition, the hADSC/NGF-hydrogel group showed increased endothelial nitric oxide synthase protein expression. This study suggests that application of hADSCs with NGF-hydrogel on the CN might be a promising treatment for postprostatectomy ED.
authors: Department of Urology, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Korea.
authors: In Gul
authors: IG
authors: Kim
authors: Shuyu
authors: S
authors: Piao
authors: Ji Young
authors: JY
authors: Lee
authors: Sung Hoo
authors: SH
authors: Hong
authors: Tae-Kon
authors: TK
authors: Hwang
authors: Sae Woong
authors: SW
authors: Kim
authors: Choung Soo
authors: CS
authors: Kim
authors: Jeong Chan
authors: JC
authors: Ra
authors: Insup
authors: I
authors: Noh
authors: Ji Youl
authors: JY
authors: Lee
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000637:transplantation*; D000818:Animals; D002478:Cells, Cultured; D003131:Combined Modality Therapy; D004337:Drug Carriers / Q000737:chemistry; D007172:Erectile Dysfunction / Q000209:etiology* / Q000517:prevention & control*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008297:Male; D020932:Nerve Growth Factor / Q000008:administration & dosage*; D059348:Peripheral Nerve Injuries / Q000209:etiology / Q000628:therapy*; D011468:Prostatectomy / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology*; D033581:Stem Cell Transplantation / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D004337:Drug Carriers; D020100:Hydrogels; D020932:Nerve Growth Factor