title: An alternative cetyltrimethylammonium bromide-based  protocol for RNA isolation from blackberry (Rubus L.).
abstract: Isolation of high-quality RNA free of contaminants,  such as polyphenols, proteins, plant secondary metabolites, and genomic DNA  from plant tissues, is usually a challenging but crucial step for molecular  analysis. We developed a novel protocol based on the cetyltrimethylammonium  bromide method to isolate high-quality RNA from blackberry plant tissues,  especially fruits. Most DNA was removed when acetic acid was utilized, before RNA  precipitation. Thus, lithium chloride, a reagent widely used for RNA  purification, was not needed. The isolation time was shortened to less than 3  h. The RNA was quite pure, with little DNA contamination. The quality of the  RNA was assessed by spectrophotometric readings and electrophoresis on agarose  gels. It was good enough for downstream enzymatic reactions, such as reverse  transcription-PCR, cloning and real-time PCR assay. The method yielded an  amount of total RNA comparable to previously described protocols.
authors: College of Horticulture, Sichuan Agricultural University, Sichuan, PR China.
authors: Q
authors: Q
authors: Chen
authors: H W
authors: HW
authors: Yu
authors: X R
authors: XR
authors: Wang
authors: X L
authors: XL
authors: Xie
authors: X Y
authors: XY
authors: Yue
authors: H R
authors: HR
authors: Tang
mesh_terms: D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D035264:Flowers / Q000235:genetics; D005638:Fruit / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics; D018749:RNA, Plant / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D027824:Rosaceae / Q000235:genetics*
chemical_list: D002593:Cetrimonium Compounds; D003902:Detergents; D018749:RNA, Plant; D000077286:Cetrimonium