title: Rheinheimera tilapiae sp. nov., isolated from a freshwater culture pond.
abstract: A bacterial strain designated Ruye-90(T) was isolated from a freshwater tilapiine cichlid fish culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Strain Ruye-90(T) was gram-negative, aerobic, yellow-coloured, rod-shaped, and motile by means of a single polar flagellum. Growth occurred at 4-30 °C (optimum, 20-30 °C), at pH 7.0-9.0 (optimum, pH 8.0-9.0) and with 0-2 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ruye-90(T) belonged to the genus Rheinheimera and its most closely related neighbour was Rheinheimera tangshanensis JA3-B52(T) with sequence similarity of 97.5 %. The major fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c, 41.3 %), C16 : 0 (19.3 %), C18 : 1ω7c (8.4 %) and C12 : 0 3-OH (7.0 %). The major respiratory quinone was Q-8. The DNA G+C content of the genomic DNA was 49.0 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, aminolipid and two uncharacterized phospholipids. The DNA-DNA relatedness of strain Ruye-90(T) with respect to recognized members of the genus Rheinheimera was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Ruye-90(T) represents a novel species in the genus Rheinheimera, for which the name Rheinheimera tilapiae sp. nov. is proposed. The type strain is Ruye-90(T) ( = LMG 26339(T) = BCRC 80263(T) = KCTC 23315(T)).
authors: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Road Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors: Wen-Ming
authors: WM
authors: Chen
authors: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Road Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors: Shwu-Harn
authors: SH
authors: Yang
authors: College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC.
authors: Chiu-Chung
authors: CC
authors: Young
authors: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Road Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors: Shih-Yi
authors: SY
authors: Sheu
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002841:Chromatiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D023681:Cichlids / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S