title: [Study on the HPLC fingerprint of toad skin from different regions].
abstract: OBJECTIVE
  To study the HPLC fingerprint of toad skin and provide a reliable method for quality control and identification.
  
  
  METHODS
  It used HPLC for detection and computer aided similarity evaluation system for processing and analysing HPLC fingerprint.
  
  
  RESULTS
  The common pattern of HPLC fingerprint of toad skin was astablished, 29 peaks were identified as the characteristic fingerprints, in which 9 peaks corresponded to 9 bufogenins. (2) Each samples' similarity of relative retention time was all above 0.99, but the similarity of relative peak areas was low.
  
  
  CONCLUSIONS
  (1) The method is accurate and with good reproducibility. The fingerprints can be used for the identification and quality control of toad skin. (2) The toad skin from different regions are stable in composition, but the contents of the components are different.
authors: Department of Natural Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China. duanlinrui@163.com
authors: Lin-rui
authors: LR
authors: Duan
authors: Xiao-kai
authors: XK
authors: Zhang
authors: Wei
authors: W
authors: Cao
authors: Yan-hua
authors: YH
authors: Xie
authors: Si-wang
authors: SW
authors: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry; D002018:Bufanolides / Q000737:chemistry*; D002025:Bufonidae; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008419:Materia Medica / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012867:Skin / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D002018:Bufanolides; D008419:Materia Medica