title: MiR-146a polymorphism is associated with asthma but not with systemic lupus erythematosus and juvenile rheumatoid arthritis in Mexican patients.
abstract: Extensive research has shown that aberrant expression of microRNAs (miRNAs) plays an important role in innate and adaptive immune responses. The rs2910164 polymorphism has been identified as a functional variant, which affects the transcription and expression level of miR-146a and, thereby, contributes to the pathogenesis of several inflammatory and autoimmune diseases. To investigate whether the rs2910164 G/C polymorphism was associated with asthma, systemic lupus erythematosus (SLE) or juvenile rheumatoid arthritis (JRA), we performed an association study in a pediatric Mexican cohort. We included 979 pediatric patients (asthma: 402, SLE: 367 and JRA: 210) and 531 control subjects without inflammatory or immune diseases. Genotyping was performed using the 5' exonuclease technique. The genotype distribution of the rs2910164 polymorphism was in Hardy-Weinberg equilibrium in each group. No significant differences were detected in the distribution of this polymorphism between cases and controls (P = 0.108, 0.609 and 0.553 for subjects with asthma, JRA and SLE, respectively). However, stratification by gender showed a statistically significant difference between asthmatic and control females, where the C allele was significantly associated with protection to asthma (odds ratio = 0.694, 95% confidence interval 0.519-0.929, P = 0.0138). Our results provide evidence that rs2910164 may play a role in the susceptibility to childhood-onset asthma, but not SLE or JRA in Mexicans. Further association studies may contribute to determining the role of miR-146a single-nucleotide polymorphisms in immune-mediated diseases.
authors: Immunogenomics and Metabolic diseases Laboratory, Instituto Nacional de Medicina Genómica, S.S, Mexico City, Mexico.
authors: S
authors: S
authors: Jiménez-Morales
authors: R
authors: R
authors: Gamboa-Becerra
authors: V
authors: V
authors: Baca
authors: B E
authors: BE
authors: Del Río-Navarro
authors: D Y
authors: DY
authors: López-Ley
authors: R
authors: R
authors: Velázquez-Cruz
authors: Y
authors: Y
authors: Saldaña-Alvarez
authors: G
authors: G
authors: Salas-Martínez
authors: L
authors: L
authors: Orozco
mesh_terms: D000293:Adolescent; D000328:Adult; D017668:Age of Onset; D000483:Alleles; D001171:Arthritis, Juvenile / Q000453:epidemiology* / Q000235:genetics / Q000276:immunology; D001249:Asthma / Q000453:epidemiology* / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000453:epidemiology* / Q000235:genetics / Q000276:immunology; D008297:Male; D008800:Mexico / Q000453:epidemiology; D035683:MicroRNAs / Q000235:genetics* / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012737:Sex Factors
chemical_list: C542542:MIRN146 microRNA, human; D035683:MicroRNAs