title: Selective immunophenotyping for diagnosis of B-cell neoplasms: immunohistochemistry and flow cytometry strategies and results.
abstract: Determining the immunophenotype of hematologic malignancies is now an indispensable part of diagnostic classification, and can help to guide therapy, or to predict clinical outcome. Diagnostic workup should be guided by morphologic findings and evaluate clinically important markers, but ideally should avoid the use of overly broad panels of immunostains that can reveal incidental findings of uncertain significance and give rise to increased costs. Here, we outline our approach to diagnosis of B-cell neoplasms, combining histologic and clinical data with tailored panels of immunophenotyping reagents, in the context of the 2008 World Health Organization classification. We present data from cases seen at our institution from 2004 through 2008 using this approach, to provide a practical reference for findings seen in daily diagnostic practice.
authors: Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA. sboyd1@stanford.edu
authors: Scott D
authors: SD
authors: Boyd
authors: Yasodha
authors: Y
authors: Natkunam
authors: John R
authors: JR
authors: Allen
authors: Roger A
authors: RA
authors: Warnke
mesh_terms: D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology / Q000473:pathology; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D007938:Leukemia / Q000145:classification / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000145:classification / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D060787:Neoplasm Grading / Q000592:standards*; D009363:Neoplasm Proteins / Q000235:genetics* / Q000276:immunology; D011379:Prognosis
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins