title: [Construction and functional studies of uropathogenic E. coli strains with ompT gene knockout].
abstract: OBJECTIVE
  To explore the role of ompT gene in uropathogenic E. coli (UPEC) CFT073 strain in urinary tract infection (UTI).
  
  
  METHODS
  An ompT deletion mutant (COTD) was generated by λ Red recombineering in the UPEC CFT073 strain, which was characterized by PCR and sequencing. C57B/L6 mouse models of acute UTI with the mutant and wild-type strains were established to compare the colonization abilities of the two strains in the bladder. The adhesion of CFT073 mutant to human unthelial 5637 cells was also investigated in vitro.
  
  
  RESULTS
  PCR and DNA sequencing confirmed the loss of ompT gene in the mutant COTD. The in vitro adhesion rate of the mutant strain COTD to 5637 cells was (6.7±2.2)%, significantly lower than that of (8.3±1.9)% of the wild-type strain (P<0.05). In the murine models of acute UTI, the mutant strain showed a mean colonization number of about (17±8)×10⁴ cfu, which was significantly lower than that of (7∓2)×10⁵ cfu of the wide-type CFT073 strain (P<0.05).
  
  
  CONCLUSIONS
  OmpT gene can be involved in the colonization of UPEC in the bladder tissue and plays an important role in the pathogenesis of UPEC-induced UTI.
authors: Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. 443801982@163.com
authors: Tie
authors: T
authors: Zhao
authors: Xingxing
authors: X
authors: Fang
authors: Xiaolu
authors: X
authors: Liu
authors: Liang
authors: L
authors: Peng
authors: Min
authors: M
authors: Long
authors: Wenbing
authors: W
authors: Zhang
authors: Jun
authors: J
authors: Luo
authors: Hong
authors: H
authors: Cao
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D002460:Cell Line; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D055786:Gene Knockout Techniques; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018272:Porins / Q000235:genetics; D014552:Urinary Tract Infections / Q000382:microbiology*; D056689:Uropathogenic Escherichia coli / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D018272:Porins; C405564:ompT protein, bacteria