title: Motility behavior of rpoS-deficient Escherichia coli analyzed by individual cell tracking.
abstract: Motility is one of the most extensively studied cellular events conducted by bacteria, including Escherichia coli. A motility agar plate assay showed that deletion of the rpoS gene enhanced the apparent motility of the E. coli BW25113 strain, which inherently had negligible motility compared to wild-type E. coli strains, such as MG1655, with no effect on cell growth. This enhancement of motility was accompanied by drastic up-regulation of genes involved in the formation and rotation of flagella. Furthermore, an individual cell motility assay showed that the population of ΔrpoS cells had bimodal motility character, and that a minority of this population exhibited a much higher motility rate. These results support a view that a minority population contributes to increasing in apparent motility of the whole population of ΔrpoS cells.
authors: Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan. ojima@cheng.es.osaka-u.ac.jp
authors: Yoshihiro
authors: Y
authors: Ojima
authors: Kazumi
authors: K
authors: Hakamada
authors: Yosuke
authors: Y
authors: Nishinoue
authors: Minh Hong
authors: MH
authors: Nguyen
authors: Jun
authors: J
authors: Miyake
authors: Masahito
authors: M
authors: Taya
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001681:Biological Assay; D058948:Cell Tracking; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics; D005407:Flagella / Q000235:genetics / Q000502:physiology; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D009068:Movement; D012808:Sigma Factor / Q000172:deficiency* / Q000235:genetics; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria