title: ZmMKK3, a novel maize group B mitogen-activated protein kinase kinase gene, mediates osmotic stress and ABA signal responses.
abstract: Mitogen-activated protein kinase (MAPK) cascades are important intracellular signaling modules and function as a convergent point for crosstalk during abiotic stress signaling. In this article, we isolated a novel group B MAPKK gene, ZmMKK3, from Zea mays. ZmMKK3 protein might be localized in both the cytoplasm and the nucleus. RNA blot analysis indicated that the ZmMKK3 transcription was up-regulated by abscisic acid (ABA), hydrogen peroxide (H(2)O(2)) and PEG, and that H(2)O(2) mediated PEG-induced expression of ZmMKK3. Constitutive expression of ZmMKK3 in Nicotiana tabacum reduced H(2)O(2) accumulation under osmotic stress by affecting antioxidant defense systems and alleviated reactive oxygen species-mediated injury under oxidative stress. Transgenic tobacco exhibited attenuated ABA sensitivity by means of an increased germination rate and main root growth. Taken together, these results indicate that ZmMKK3 is a positive regulator of osmotic tolerance and ABA signaling in plants.
authors: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, China.
authors: Maoying
authors: M
authors: Zhang
authors: Jiaowen
authors: J
authors: Pan
authors: Xiangpei
authors: X
authors: Kong
authors: Yan
authors: Y
authors: Zhou
authors: Yang
authors: Y
authors: Liu
authors: Liping
authors: L
authors: Sun
authors: Dequan
authors: D
authors: Li
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D009997:Osmotic Pressure / Q000502:physiology; D030821:Plants, Genetically Modified / Q000378:metabolism; D011092:Polyethylene Glycols / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological; D014026:Nicotiana / Q000201:enzymology / Q000254:growth & development; D003313:Zea mays / Q000201:enzymology / Q000235:genetics*
chemical_list: D011092:Polyethylene Glycols; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D020928:Mitogen-Activated Protein Kinases