title: Similar evolution of cellular HIV-1 DNA level in darunavir/ritonavir monotherapy versus triple therapy in MONOI-ANRS136 trial over 96 weeks.
abstract: BACKGROUND
  A higher proportion of intermittent viremia (to have a HIV-1 RNA viral load>50 copies/mL not confirmed) was reported in the boosted protease inhibitor monotherapy arm in some studies including MONOI trial, and that could have an impact on the replenishment of the HIV-1 DNA reservoirs. The HIV-1 DNA level is an interesting marker which reflects the size of cellular HIV reservoir. Our objectives were to study the impact of 96 weeks of Darunavir/ritonavir monotherapy versus a triple standard combination on the HIV-1 blood reservoir and factors associated with HIV-1 plasma DNA at baseline in MONOI trial sub-study.
  
  
  RESULTS
  This sub-study is focused on 160 patients (79 patients in monotherapy arm and 81 in tritherapy arm) for whom blood cells were available both at baseline and at week 96 (W96). Baseline HIV-1 plasma DNA was associated with CD4 nadir, pre therapeutic HIV-1 RNA viral load and baseline HIV-1 RNA measured by ultrasensitive assay. A similar median delta HIV-DNA was observed between D0 and W96 in both arms; 0.35 log copies/10(6) leucocytes in monotherapy arm versus 0.51 log copies/10(6) leucocytes in tritherapy arm.
  
  
  CONCLUSIONS
  Despite a higher proportion of intermittent viremia in monotherapy arm, a similar evolution of cellular HIV-1 DNA level was observed between mono and triple therapy arm.
  
  
  BACKGROUND
  ClinicalTrials. gov NCT00421551.
authors: AP-HP, Pitié-Salpêtrière Hospital, INSERM U 943 and Pierre et Marie Curie University Paris, Paris, France. sidonie.lambert@psl.aphp.fr
authors: Sidonie
authors: S
authors: Lambert-Niclot
authors: Philippe
authors: P
authors: Flandre
authors: Marc-Antoine
authors: MA
authors: Valantin
authors: Cathia
authors: C
authors: Soulie
authors: Slim
authors: S
authors: Fourati
authors: Marc
authors: M
authors: Wirden
authors: Sophie
authors: S
authors: Sayon
authors: Sophie
authors: S
authors: Pakianather
authors: Laurence
authors: L
authors: Bocket
authors: Bernard
authors: B
authors: Masquelier
authors: Georges
authors: G
authors: Dos Santos
authors: Christine
authors: C
authors: Katlama
authors: Vincent
authors: V
authors: Calvez
authors: Anne-Genevieve
authors: AG
authors: Marcelin
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000097:blood*; D000069454:Darunavir; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000188:drug therapy / Q000235:genetics; D017320:HIV Protease Inhibitors / Q000008:administration & dosage*; D015497:HIV-1; D006801:Humans; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000097:blood*; D019438:Ritonavir / Q000008:administration & dosage*; D013449:Sulfonamides / Q000008:administration & dosage*; D019562:Viral Load / Q000187:drug effects; D014766:Viremia / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D017320:HIV Protease Inhibitors; D012367:RNA, Viral; D013449:Sulfonamides; D019438:Ritonavir; D000069454:Darunavir