title: Nitric oxide modulated the expression of DREAM/calsenilin/KChIP3 in inflammatory pain of rats.
abstract: Downstream regulatory element antagonistic modulator (DREAM) is a critical transcriptional repressor for pain modulation. The role of nitric oxide (NO) plays in modulating DREAM pain pathway in the periphery is unclear. Therefore, we investigated the role of the NO in modulation of the expression of DREAM in formalin-induced rat inflammatory pain models. Male Sprague-Dawley rats were randomly distributed into four groups: the normal group, formalin test group, Nω-nitro-L-arginine (l-NNA) group, and morphine group. One hundred microliters of 2.5 % formalin was injected into the plantar surface of the right hindpaw of rats. l-NNA (40 nmol/L) and morphine (40 nmol/L) were injected intrathecally in the hindpaw before formalin injection. The nociceptive behavioral reaction was recorded. After the formalin test, the expression of DREAM mRNA and protein in the spinal cord of the four groups were measured. The nociceptive reaction induced by injection of formalin exhibited two phases. Morphine and l-NNA significantly decreased pain scores of the second phase. The expression of DREAM was significantly increased in the rat spinal cord after formalin-induced pain. Morphine significantly upregulated the expression of DREAM, and the formalin-induced upregulation was significantly attenuated by l-NNA. NO may play an important role in the DREAM pathway modulation of inflammatory pain.
authors: Department of Physiology, Harbin Medical University, 194 Xuefu Rd, Nangang District, Harbin 150081, People's Republic of China.
authors: Hong-Bo
authors: HB
authors: Jin
authors: Yong-Liang
authors: YL
authors: Yang
authors: Ying-Li
authors: YL
authors: Song
authors: Yong-Bin
authors: YB
authors: Yang
authors: Yu-Rong
authors: YR
authors: Li
mesh_terms: D000818:Animals; D005557:Formaldehyde; D007249:Inflammation / Q000139:chemically induced / Q000503:physiopathology*; D007278:Injections, Spinal; D051605:Kv Channel-Interacting Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009020:Morphine / Q000494:pharmacology / Q000627:therapeutic use; D009569:Nitric Oxide / Q000378:metabolism*; D019335:Nitroarginine / Q000494:pharmacology / Q000627:therapeutic use; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology*; D010147:Pain Measurement; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism
chemical_list: C496760:Kcnip3 protein, rat; D051605:Kv Channel-Interacting Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D005557:Formaldehyde; D019335:Nitroarginine; D009569:Nitric Oxide; D009020:Morphine