title: Pressurized liquid extraction and HPLC quantification of folic acid in fortified wheat flours.
abstract: A pressurized liquid extraction (PLE) method using phosphate buffer as solvent was applied for folic acid (FA) extraction from fortified wheat flours and was compared to a standard solid-liquid extraction (SLE) method. Extracted FA was quantified by reverse phase high-performance liquid chromatography (RP-HPLC) hyphenated with a phenyl column and an absorption photometric detector (λ = 280 nm). Detection and quantification limits were 0.12 and 0.4 ng, respectively, corresponding to 0.06 and 0.2 μg g(-1) of analyzed wheat flour. Equivalent FA contents were found by both extraction methods, but a single PLE allowed a total recovery of FA content, whereas at least three successive SLEs were needed to achieve a total recovery of FA. The obtained results indicated that PLE is a rapid and efficient technique for FA extraction from fortified wheat flour.
authors: Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP), Centro de Tecnologia das Radiações. Av. Prof. Lineu Prestes 2242, 05508-910 São Paulo, Brazil. mmozeika@yahoo.com
authors: Michel Mozeika
authors: MM
authors: Araújo
authors: Eric
authors: E
authors: Marchioni
authors: Anna Lucia Casañas Haasis
authors: AL
authors: Villavicencio
authors: Minjie
authors: M
authors: Zhao
authors: Pierre
authors: P
authors: Zimmermann
authors: Etienne
authors: E
authors: El-Khoury
authors: Martine
authors: M
authors: Bergaentzle
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005433:Flour / Q000032:analysis*; D005492:Folic Acid / Q000032:analysis / Q000302:isolation & purification*; D005527:Food, Fortified / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D014908:Triticum / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D005492:Folic Acid