title: Wollastonite nanofiber-doped self-setting calcium phosphate bioactive cement for bone tissue regeneration.
abstract: The purpose of this study was to synthesize a self-setting bioactive cement by incorporation of wollastonite nanofibers (WNFs) into calcium phosphate cement (CPC). The composition, morphology, setting time, compressive strength, hydrophilicity, and degradation of WNF-doped CPC (wnf-CPC) were investigated. Scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and inductively coupled plasma atomic emission spectroscopy were utilized. Additionally, methyl-thiazolyl-tetrazolium bromide assay, scanning electron microscopy, inductively coupled plasma atomic emission spectroscopy, and histological evaluation were used to study the cell and tissue responses to wnf-CPC, both in vitro and in vivo. The results confirmed that the addition of WNFs into CPC had no obvious effect on the setting time or the compressive strength of wnf-CPC, provided the WNF amount was not more than 10 wt%. However, the hydrophilicity and degradability of wnf-CPC were significantly improved by the addition of WNFs - this was because of the change of microstructure caused by the WNFs. The preferred dissolution of WNFs caused the formation of microporosity in wnf-CPC when soaked in tris hydrochloride solution. The microporosity enlarged the surface area of the wnf-CPC and so promoted degradation of the wnf-CPC when in contact with liquid. In addition, MG-63 cell attachment and proliferation on the wnf-CPC were superior to that on the CPC, indicating that incorporation of WNFs into CPC improved the biological properties for wnf-CPC. Following the implantation of wnf-CPC into bone defects of rabbits, histological evaluation showed that wnf-CPC enhanced the efficiency of new bone formation in comparison with CPC, indicating excellent biocompatibility and osteogenesis of wnf-CPC. In conclusion, wnf-CPC exhibited promising prospects in bone regeneration.
authors: Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, People's Republic of China.
authors: Han
authors: H
authors: Guo
authors: Jie
authors: J
authors: Wei
authors: Wenhua
authors: W
authors: Song
authors: Shan
authors: S
authors: Zhang
authors: Yonggang
authors: Y
authors: Yan
authors: Changsheng
authors: C
authors: Liu
authors: Tiqiao
authors: T
authors: Xiao
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D017610:Calcium Compounds / Q000737:chemistry* / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002468:Cell Physiological Phenomena / Q000187:drug effects; D004355:Drug Stability; D005269:Femur / Q000166:cytology / Q000293:injuries; D006651:Histocytochemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D010012:Osteogenesis; D010316:Particle Size; D011817:Rabbits; D017640:Silicates / Q000737:chemistry* / Q000494:pharmacology; D023822:Tissue Engineering; D014945:Wound Healing / Q000187:drug effects
chemical_list: D017610:Calcium Compounds; D002130:Calcium Phosphates; D017640:Silicates; C020243:calcium phosphate; C031293:calcium silicate