title: Distribution and accumulation of caffeine in rat tissues and its inhibition on semicarbazide-sensitive amine oxidase.
abstract: Wistar rats were treated with caffeine or 2-bromoethylamine, the effect of caffeine on the activity of semicarbazide-sensitive amine oxidase (SSAO) in rat serum and tissues was studied using various LC-MS methods. Caffeine was found to present in all tissues after administration for 10 days and accumulated for 25 days. The level of caffeine was high in brain and liver, and the SSAO activity in all tissues was found to be inhibited by caffeine. As the concentration of caffeine increased, the SSAO activity decreased. The inhibition ratio was correlated to the levels of caffeine present. We presume that caffeine may treat with SSAO activity associated diseases.
authors: School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
authors: Baoquan
authors: B
authors: Che
authors: Lin
authors: L
authors: Wang
authors: Zhe
authors: Z
authors: Zhang
authors: Yongqian
authors: Y
authors: Zhang
authors: Yulin
authors: Y
authors: Deng
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000201:enzymology; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002110:Caffeine / Q000097:blood / Q000493:pharmacokinetics*; D000697:Central Nervous System Stimulants / Q000097:blood / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D005021:Ethylamines / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D000697:Central Nervous System Stimulants; D005021:Ethylamines; C004504:2-bromoethylamine; D002110:Caffeine; D006631:Amine Oxidase (Copper-Containing)