title: A FILAMENTOUS FLOWER orthologue plays a key role in leaf patterning in opium poppy.
abstract: The plant-specific YABBY genes were initially defined by their roles in determining abaxial/adaxial cell fate in lateral organs of eudicots, and repressing meristematic genes in differentiating tissues such as leaves. In Arabidopsis thaliana FILAMENTOUS FLOWER (FIL) is also required for inflorescence and floral meristem establishment and flower development in a pathway involving the floral transition and identity genes. Here we describe the characterization of a FIL orthologue from the basal eudicot, Papaver somniferum (the opium poppy), and demonstrate a role for the gene in patterning the highly lobed leaf of the poppy. Silencing of PapsFIL using viral-induced gene silencing resulted in leaves of reduced laminar area, more pronounced margin serration and, in some cases, leaf bifurcation. In contrast, the gene does not appear to affect the development of the flower, and these variations in function are discussed in relation to its taxonomic position as a basal eudicot and its determinate growth habit.
authors: Department of Biology, University of Leicester, University Road, Leciester LE1 7RH, UK.
authors: Nikolaos
authors: N
authors: Vosnakis
authors: Afiqah
authors: A
authors: Maiden
authors: Sofia
authors: S
authors: Kourmpetli
authors: Philip
authors: P
authors: Hands
authors: Donna
authors: D
authors: Sharples
authors: Sinéad
authors: S
authors: Drea
mesh_terms: D060054:Agrobacterium / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D017796:Caulimovirus / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D035264:Flowers / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D017343:Genes, Plant; D018519:Meristem / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D010207:Papaver / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic
chemical_list: C119103:AFO protein, Arabidopsis; D029681:Arabidopsis Proteins; D010940:Plant Proteins