title: Bacteriological analysis of platelets and cases of septic reactions associated with transfusion of contaminated samples.
abstract: BACKGROUND
  For years, platelet transfusion therapy has been playing an important role in controlling patients with hematological and oncological diseases. However, platelet transfusion represents a serious risk for bacterial sepsis. This study aimed to verify the bacterial contamination index in platelet concentrates (PCs) obtained from the Blood Center of the State of Rio Grande do Sul (HEMORGS).
  
  
  METHODS
  All 612 samples of PCs from HEMORGS were analyzed in August 2009 and January 2010. We used a qualitative methodology which was modified manually in order to detect bacterial contamination. The patients who received the platelet units with confirmed positive cultures had their medical records analyzed. After the prospective study, a retrospective analysis of the samples of PCs checked by the bacteriological control from HEMORGS since its foundation (2008) until 2010 was made.
  
  
  RESULTS
  Four hundred and eighty samples (10 monthly samples of plateletpheresis and 10 of whole blood-derived platelets) were analyzed in order to compare the contamination index for both studies. Of 1092 samples, 15 were found to be contaminated (6 of retrospective analysis and 9 of prospective). In prospective study, isolated microorganisms were nine Staphylococcus epidermidis, but in retrospective two Staphylococcus sp., two glucose non-fermenting gram negative bacilli, one Streptococcus sp. and one Proteus mirabilis. We report 3 feasible cases of bacterial sepsis associated with the transfusion of CPs analyzed by prospective study.
  
  
  CONCLUSIONS
  Bacterial sepsis associated with platelet transfusion is today the most frequent infectious complication of transfusion medicine. Although complex, it is important to recognize transfusion sepsis in patients to reduce morbidity and mortality.
authors: Post-Graduation in Pharmaceutical Sciences Program, Federal University of Santa Maria, Campus of UFSM, 97015-900 Santa Maria, RS, Brazil. rosifarma@gmail.com
authors: Rosiéli
authors: R
authors: Martini
authors: Rosmari
authors: R
authors: Horner
authors: Mônica de Abreu
authors: Mde A
authors: Rodrigues
authors: Cláudia Barbisan
authors: CB
authors: Kempfer
authors: Maísa Kraulich
authors: MK
authors: Tizotti
authors: Viviane
authors: V
authors: Ratzlaff
mesh_terms: D000293:Adolescent; D016470:Bacteremia / Q000097:blood / Q000209:etiology* / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods; D001792:Blood Platelets / Q000382:microbiology*; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D017713:Platelet Transfusion / Q000009:adverse effects*; D013203:Staphylococcal Infections / Q000097:blood / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000302:isolation & purification; D065227:Transfusion Reaction; D055815:Young Adult