title: Mechanical and kinetic properties of β-cardiac/slow skeletal muscle myosin.
abstract: We aimed to establish reference parameters to identify functional effects of familial hypertrophic cardiomyopathy-related point mutations in the β-cardiac/slow skeletal muscle myosin heavy chain (β-cardiac/MyHC-1). We determined mechanical and kinetic parameters of the β-cardiac/MyHC-1 using human soleus muscle fibers that express the same myosin heavy chain (MyHC-1) as ventricular myocardium (β-cardiac). The observed parameters are compared to previously reported data for rabbit psoas muscle fibers. We found all of the examined kinetic parameters to be slower in soleus fibers than in rabbit psoas muscle. Somewhat surprisingly, however, we also found that the stiffness of the β-cardiac/MyHC-1 head domain is more than 3-fold lower than the stiffness of the fast isoform of psoas fibers. Furthermore, and different from rabbit psoas muscle, in human soleus fibers both the occupancy of force-generating cross-bridge states as well as the elastic extension of force-generating heads increase with temperature. Thus, a myosin head in the force generating states makes an increasing contribution to force with temperature. We support some of our fiber data by data from in vitro motility and optical trapping assays. Initial findings with FHC-related point mutations in the converter imply that the differences in stiffness of the head domain between the slow and fast isoform may well be due to particular differences in the amino acid sequence of the converter. We show that the slower kinetics may be linked to a larger flexibility of the β-cardiac/MyHC-1 isoform compared to fast MyHC isoforms.
authors: Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. brenner.bernhard@mh-hannover.de
authors: Bernhard
authors: B
authors: Brenner
authors: Nils
authors: N
authors: Hahn
authors: Eva
authors: E
authors: Hanke
authors: Faramarz
authors: F
authors: Matinmehr
authors: Tim
authors: T
authors: Scholz
authors: Walter
authors: W
authors: Steffen
authors: Theresia
authors: T
authors: Kraft
mesh_terms: D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D018482:Muscle, Skeletal / Q000378:metabolism; D024744:Skeletal Muscle Myosins / Q000378:metabolism*; D013696:Temperature
chemical_list: D000199:Actins; D000251:Adenosine Triphosphatases; D024744:Skeletal Muscle Myosins