title: Composition and organization of active centromere sequences in complex genomes.
abstract: BACKGROUND
  Centromeres are sites of chromosomal spindle attachment during mitosis and meiosis. While the sequence basis for centromere identity remains a subject of considerable debate, one approach is to examine the genomic organization at these active sites that are correlated with epigenetic marks of centromere function.
  
  
  RESULTS
  We have developed an approach to characterize both satellite and non-satellite centromeric sequences that are missing from current assemblies in complex genomes, using the dog genome as an example. Combining this genomic reference with an epigenetic dataset corresponding to sequences associated with the histone H3 variant centromere protein A (CENP-A), we identify active satellite sequence domains that appear to be both functionally and spatially distinct within the overall definition of satellite families.
  
  
  CONCLUSIONS
  These findings establish a genomic and epigenetic foundation for exploring the functional role of centromeric sequences in the previously sequenced dog genome and provide a model for similar studies within the context of less-characterized genomes.
authors: Genome Biology Group, Duke Institute for Genome Sciences & Policy, Duke University, Durham, NC, USA. kehayden@soe.ucsc.edu
authors: Karen E
authors: KE
authors: Hayden
authors: Huntington F
authors: HF
authors: Willard
mesh_terms: D000818:Animals; D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D047369:Chromatin Immunoprecipitation; D004251:DNA Transposable Elements / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics; D030541:Databases, Genetic; D004285:Dogs; D015723:Gene Library; D016678:Genome / Q000235:genetics*; D061985:Madin Darby Canine Kidney Cells; D058977:Molecular Sequence Annotation
chemical_list: D004251:DNA Transposable Elements; D004276:DNA, Satellite