title: The tomato yellow leaf curl virus (TYLCV) V2 protein interacts with the host papain-like cysteine protease CYP1.
abstract: The V2 protein of Tomato yellow leaf curl geminivirus (TYLCV) is an RNA-silencing suppressor that counteracts the innate immune response of the host plant. However, this anti-host defense function of V2 may include targeting of other defensive mechanisms of the plant. Specifically, we show that V2 recognizes and directly binds the tomato CYP1 protein, a member of the family of papain-like cysteine proteases which are involved in plant defense against diverse pathogens. This binding occurred both in vitro and in vivo, within living plant cells. The V2 binding site within mCYP1 was identified in the direct proximity to the papain-like cysteine protease active site.
authors: Institute of Plant Sciences; A.R.O., The Volcani Center, Bet Dagan, Israel.
authors: Amalia
authors: A
authors: Bar-Ziv
authors: Yael
authors: Y
authors: Levy
authors: Hagit
authors: H
authors: Hak
authors: Anahit
authors: A
authors: Mett
authors: Eduard
authors: E
authors: Belausov
authors: Vitaly
authors: V
authors: Citovsky
authors: Yedidya
authors: Y
authors: Gafni
mesh_terms: D000595:Amino Acid Sequence; D053839:Begomovirus / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000821:virology*; D008969:Molecular Sequence Data; D010206:Papain / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014764:Viral Proteins; D010206:Papain