title: Rotavirus capsid surface protein VP4-coated Fe(3)O(4) nanoparticles as a theranostic platform for cellular imaging and drug delivery.
abstract: The development of a theranostic nanoplatform based on rotavirus structural protein VP4-coated Fe(3)O(4) nanoparticles (NPs) for dual modality magnetic resonance/fluorescence cellular imaging and drug delivery is reported. VP4 protein was obtained from Escherichia coli approach, and then chemically conjugated to Fe(3)O(4) NPs premodified with meso-2,3-dimercaptosuccinnic acid (DMSA) in the presence of 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide (EDC). Next, the VP4-coated Fe(3)O(4) NPs were loaded with doxorubicin (DOX), a typical anticancer drug, via formation of amide bond through the EDC approach. Prussian blue staining analysis reveals that the VP4-coated Fe(3)O(4) NPs can be internalized efficiently by MA104 and HepG2 cells, thereby significantly improving cellular MRI sensitivity, compared with dextran- and BSA-coated Fe(3)O(4) NPs. In addition, DOX loaded on the VP4-coated Fe(3)O(4) NPs exhibits significant cytotoxicity to the cancer cells (HepG2). The current work provides a general approach toward the rational design and synthesis of a versatile theranostic nanoplatform based on functional protein-coated magnetic NPs with good biocompatibility, biodegradability, and capability of simultaneously performing multimodality imaging and therapy for optimal clinical outcomes.
authors: Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, PR China.
authors: Weihong
authors: W
authors: Chen
authors: Yuhua
authors: Y
authors: Cao
authors: Min
authors: M
authors: Liu
authors: Qinghuan
authors: Q
authors: Zhao
authors: Jie
authors: J
authors: Huang
authors: Hailu
authors: H
authors: Zhang
authors: Zongwu
authors: Z
authors: Deng
authors: Jianwu
authors: J
authors: Dai
authors: David F
authors: DF
authors: Williams
authors: Zhijun
authors: Z
authors: Zhang
mesh_terms: D000818:Animals; D002213:Capsid / Q000737:chemistry*; D036022:Capsid Proteins / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D003952:Diagnostic Imaging / Q000379:methods*; D004317:Doxorubicin / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005290:Ferric Compounds / Q000737:chemistry / Q000633:toxicity; D006801:Humans; D007118:Immunoassay; D008279:Magnetic Resonance Imaging; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D013499:Surface Properties / Q000187:drug effects
chemical_list: D036022:Capsid Proteins; D005290:Ferric Compounds; C060339:VP4 protein, Rotavirus; C000499:ferric oxide; D004317:Doxorubicin