title: Stress-induced interaction between p38 MAPK and HSP70.
abstract: p38 MAPK, one of the four MAPK subfamilies in mammalian cells, is activated by environmental stresses and pro-inflammatory cytokines, playing fundamental roles in many biological processes. Despite all that is known on the structure and functions of p38, many questions still exist. The coupling of activation and nuclear translocation represents an important aspect of p38 signaling. In our effort in exploring the potential chaperone for p38 translocation, we performed an endogenous pull-down assay and identified HSP70 as a potential interacting protein of p38. We confirmed the interaction between p38 and HSP70 in vitro and in vivo, and identified their interaction domains. We also showed stress-induced nuclear co-localization of these two proteins. Our preliminary result indicated that HSP70 was related to the phosphorylation of MK2, a specific nuclear downstream target of p38, suggesting HSP70 is a potential chaperone for the nuclear translocation of p38.
authors: Department of Pathophysiology and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou 510515, China. gongxw@fimmu.com
authors: Xiaowei
authors: X
authors: Gong
authors: Tingting
authors: T
authors: Luo
authors: Peng
authors: P
authors: Deng
authors: Zhenxi
authors: Z
authors: Liu
authors: Jiancheng
authors: J
authors: Xiu
authors: Hongqin
authors: H
authors: Shi
authors: Yong
authors: Y
authors: Jiang
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D010766:Phosphorylation; D054730:Protein Interaction Domains and Motifs; D060066:Protein Interaction Maps; D013312:Stress, Physiological; D048051:p38 Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D048051:p38 Mitogen-Activated Protein Kinases