title: The interfascicular trigeminal nucleus: a precerebellar nucleus in the mouse defined by retrograde neuronal tracing and genetic fate mapping.
abstract: We have found a previously unreported precerebellar nucleus located among the emerging fibers of the motor root of the trigeminal nerve in the mouse, which we have called the interfascicular trigeminal nucleus (IF5). This nucleus had previously been named the tensor tympani part of the motor trigeminal nucleus (5TT) in rodent brain atlases, because it was thought to be a subset of small motor neurons of the motor trigeminal nucleus innervating the tensor tympani muscle. However, following injection of retrograde tracer in the cerebellum, the labeled neurons in IF5 were found to be choline acetyltransferase (ChAT) negative, indicating that they are not motor neurons. The cells of IF5 are strongly labeled in mice from Wnt1Cre and Atoh1 CreER lineage fate mapping, in common with the major precerebellar nuclei that arise from the rhombic lip and that issue mossy fibers. Analysis of sections from mouse Hoxa3, Hoxb1, and Egr2 Cre labeled lineages shows that the neurons of IF5 arise from rhombomeres caudal to rhombomere 4, most likely from rhombomeres 6-8. We conclude that IF5 is a significant precerebellar nucleus in the mouse that shares developmental gene expression characteristics with mossy fiber precerebellar nuclei that arise from the caudal rhombic lip.
authors: Neuroscience Research Australia, Randwick, New South Wales 2031, Australia.
authors: Yuhong
authors: Y
authors: Fu
authors: Petr
authors: P
authors: Tvrdik
authors: Nadja
authors: N
authors: Makki
authors: Robert
authors: R
authors: Machold
authors: George
authors: G
authors: Paxinos
authors: Charles
authors: C
authors: Watson
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D019070:Cell Lineage / Q000235:genetics; D002531:Cerebellum / Q000166:cytology / Q000196:embryology / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D051767:Early Growth Response Protein 2 / Q000235:genetics / Q000378:metabolism; D004525:Efferent Pathways / Q000166:cytology* / Q000196:embryology* / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009412:Nerve Fibers / Q000502:physiology; D056325:Neuronal Tract-Tracers; D011149:Pons / Q000166:cytology* / Q000196:embryology* / Q000502:physiology; D014278:Trigeminal Nuclei / Q000166:cytology* / Q000196:embryology* / Q000502:physiology; D051155:Wnt1 Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002795:Choline O-Acetyltransferase; D051767:Early Growth Response Protein 2; D049452:Green Fluorescent Proteins; D018398:Homeodomain Proteins; D056325:Neuronal Tract-Tracers; D051155:Wnt1 Protein; C500943:Atoh1 protein, mouse; C497327:Egr2 protein, mouse; C101121:HOXB1 homeodomain protein; C080331:Hoxa3 protein, mouse; C495246:Wnt1 protein, mouse; C485184:enhanced green fluorescent protein