title: E-NTPDase and E-ADA activities are altered in lymphocytes of patients with indeterminate form of Chagas' disease.
abstract: Trypanosoma cruzi infection triggers a chronic inflammatory process in human host and purinergic system ecto-enzymes play an important role in modulating the inflammatory and immune responses. In this study, it was investigated ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5; CD39) and ecto-adenosine deaminase (E-ADA; EC 3.5.4.4) activities in lymphocytes from patients with indeterminate form of Chagas' disease (IFCD). Twenty-five IFCD patients and 25 healthy subjects (control group) were selected. The peripheral lymphocytes were isolated and E-NTPDase and E-ADA activities were determined. Adenine nucleotides and adenosine levels were determined in serum by HPLC and the E-NTPDase1 expression in lymphocytes by Western blot analysis. E-NTPDase (ATP and ADP as substrates) and E-ADA (adenosine as substrate) activities were decreased in lymphocytes from IFCD patients (P<0.05 and P<0.01, respectively), while the E-NTPDase1 expression presented no changes in these patients. Serum ATP levels showed to be decreased (P<0.05) and both AMP (P<0.01) and adenosine (P<0.001) levels were increased in the IFCD group. The enzymatic alterations observed are in agreement with the immune response against T. cruzi infection in IFCD patients, since the decreased extracellular ATP and the increased adenosine levels trigger a Th2 anti-inflammatory response, which it is associated to adaptation of host to parasite, preventing clinical progress of disease.
authors: Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, 97105-900, Santa Maria, RS, Brazil.
authors: Viviane do Carmo Gonçalves
authors: Vdo C
authors: Souza
authors: Karine Bizzi
authors: KB
authors: Schlemmer
authors: Cristiano Bicca
authors: CB
authors: Noal
authors: Jeandre Augusto dos Santos
authors: JA
authors: Jaques
authors: Carine Eloise Prestes
authors: CE
authors: Zimmermann
authors: Cláudio Alberto Martins
authors: CA
authors: Leal
authors: Juliana
authors: J
authors: Fleck
authors: Emerson Andre
authors: EA
authors: Casali
authors: Vera Maria
authors: VM
authors: Morsch
authors: Maria Rosa Chitolina
authors: MR
authors: Schetinger
authors: Daniela Bitencourt Rosa
authors: DB
authors: Leal
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001081:Apyrase / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D014355:Chagas Disease / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged
chemical_list: D015703:Antigens, CD; D000243:Adenosine Deaminase; D001081:Apyrase; C067869:CD39 antigen