title: Bacterial isolates from the urine of cattle affected by urothelial tumors of the urinary bladder.
abstract: Microbiological investigations were performed on urine samples from 108 cows affected by urothelial tumors of the urinary bladder. Bacteria, frequently of mixed population, were isolated from 100 animals. Gram-positive bacteria prevailed, with Staphylococcus spp. and Bacillus spp. being the most common. Escherichia coli and Acinetobacter spp. were the most frequently recovered Gram-negative bacteria. E5 oncoprotein was detected in 86 of the 108 urothelial tumors under study. In the majority of cases, bacterial agents and BPV-2 E5 were simultaneously detected. A marked down-regulation of Tamm-Horsfall protein was also observed in the examined cases. In addition, the p65 subunit of the nuclear factor-κB (NF-κB) transcription factor appeared to be overexpressed. In all cases, a mild to severe chronic inflammation was evident in the stroma of urinary bladder tumors. Bacterial components may play a role in the activation of the NF-κB and might cause chronic inflammation resulting in an impaired ability to clear BPV-2 infection, thus cooperating with the virus in cancer development. As in man, therefore, bacteria could play both a direct and an indirect role in bovine bladder carcinogenesis.
authors: Department of Pathology and Animal Health, Division of Infectious Diseases, Faculty of Veterinary Medicine, University of Naples Federico II, Via Delpino, 1-80137 Naples, Italy.
authors: Sante
authors: S
authors: Roperto
authors: Giovanni
authors: G
authors: Di Guardo
authors: Leonardo
authors: L
authors: Leonardi
authors: Ugo
authors: U
authors: Pagnini
authors: Emmanuele
authors: E
authors: Manco
authors: Orlando
authors: O
authors: Paciello
authors: Iolanda
authors: I
authors: Esposito
authors: Giuseppe
authors: G
authors: Borzacchiello
authors: Valeria
authors: V
authors: Russo
authors: Paola
authors: P
authors: Maiolino
authors: Franco
authors: F
authors: Roperto
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D001437:Bacteriuria / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology / Q000473:pathology*; D005260:Female; D001749:Urinary Bladder Neoplasms / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D019459:Urothelium / Q000473:pathology*