title: Controversies about the chromosomal stability of cultivated mesenchymal stem cells: their clinical use is it safe?
abstract: The usefulness of adult stem cells in research and therapeutic applications highly relies on their genomic integrity and stability. Many laboratories including ours have addressed this concern using methods such as karyotyping, Qbanding, fluorescent in situ hybridization, array CGH, flow cytometry and Pap test to evaluate number and structure of chromosomes and cellular phenotype. This review attempts to summarize the findings reported so far for the studies on chromosomal aberrations in adult stem cells and warrant to perform certain basic tests before transplantation to avoid any adverse reactions, which will thus aid in better therapeutic output after cellular transplantation in the treatment of various diseases.
authors: Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Curitiba, Paraná, Brazil.
authors: Reginaldo Justino
authors: RJ
authors: Ferreira
authors: Ana Carolina
authors: AC
authors: Irioda
authors: Ricardo Correa
authors: RC
authors: Cunha
authors: Julio Cesarm
authors: JC
authors: Francisco
authors: Luiz Cesar
authors: LC
authors: Guarita-Souza
authors: Garikipati Venkata Naga
authors: GV
authors: Srikanth
authors: Soniya
authors: S
authors: Nityanand
authors: Roberto
authors: R
authors: Rosati
authors: Juan Carlos
authors: JC
authors: Chachques
authors: Katherine Athayde Teixeira
authors: KA
authors: de Carvalho
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D018929:Cell Culture Techniques; D043171:Chromosomal Instability; D002869:Chromosome Aberrations; D005820:Genetic Testing; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D044968:Regenerative Medicine