title: Magnetic responsive hydroxyapatite composite scaffolds construction for bone defect reparation.
abstract: BACKGROUND
  In recent years, interest in magnetic biomimetic scaffolds for tissue engineering has increased considerably. A type of magnetic scaffold composed of magnetic nanoparticles (MNPs) and hydroxyapatite (HA) for bone repair has been developed by our research group.
  
  
  OBJECTIVE
  In this study, to investigate the influence of the MNP content (in the scaffolds) on the cell behaviors and the interactions between the magnetic scaffold and the exterior magnetic field, a series of MNP-HA magnetic scaffolds with different MNP contents (from 0.2% to 2%) were fabricated by immersing HA scaffold into MNP colloid. ROS 17/2.8 and MC3T3-E1 cells were cultured on the scaffolds in vitro, with and without an exterior magnetic field, respectively. The cell adhesion, proliferation and differentiation were evaluated via scanning electron microscopy; confocal laser scanning microscopy; and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase, and bone gla protein activity tests.
  
  
  RESULTS
  The results demonstrated the positive influence of the magnetic scaffolds on cell adhesion, proliferation, and differentiation. Further, a higher amount of MNPs on the magnetic scaffolds led to more significant stimulation.
  
  
  CONCLUSIONS
  The magnetic scaffold can respond to the exterior magnetic field and engender some synergistic effect to intensify the stimulating effect of a magnetic field to the proliferation and differentiation of cells.
authors: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, People's Republic of China.
authors: Xiao Bo
authors: XB
authors: Zeng
authors: Hao
authors: H
authors: Hu
authors: Li Qin
authors: LQ
authors: Xie
authors: Fang
authors: F
authors: Lan
authors: Wen
authors: W
authors: Jiang
authors: Yao
authors: Y
authors: Wu
authors: Zhong Wei
authors: ZW
authors: Gu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D053761:Nanocomposites / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D015675:Osteocalcin / Q000378:metabolism; D051381:Rats; D018709:Statistics, Nonparametric; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D058185:Magnetite Nanoparticles; D015675:Osteocalcin; D017886:Durapatite; D000469:Alkaline Phosphatase