title: [Recombinant interferon-alpha suppression of Karelian fever virus replication in human blood cells].
abstract: The active replication of Karelian fever virus (KFV) in human blood vessels and the protective activity of the Russian agent reaferon were first shown. KFL was highly susceptible to interferon (IFN)-alpha. In control (uninfected) cells, reaferon caused low gene expressions of the IFN-dependent enzymes dsRNA-dependent protein kinase and 2'5'-oligoadenylate synthetase, by exerting a little effect on the activity of its family genes. KFV suppressed the reaferon-induced gene expression of IFN-dependent enzymes, but IFN-alpha gene transcription was increased in the reaferon-treated infected cells.
authors: T M
authors: TM
authors: Sokolova
authors: A N
authors: AN
authors: Shuvalov
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D018354:Alphavirus Infections / Q000378:metabolism; D000818:Animals; D001773:Blood Cells / Q000378:metabolism / Q000821:virology*; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D011994:Recombinant Proteins / Q000378:metabolism / Q000494:pharmacology; D012845:Sindbis Virus / Q000187:drug effects* / Q000302:isolation & purification; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*; D019892:eIF-2 Kinase / Q000378:metabolism
chemical_list: D000077190:Interferon alpha-2; D016898:Interferon-alpha; D012367:RNA, Viral; D011994:Recombinant Proteins; D019892:eIF-2 Kinase; D015088:2',5'-Oligoadenylate Synthetase