title: Solution structure of the second RRM domain of RBM5 and its unusual binding characters for different RNA targets.
abstract: The RNA binding motif protein 5 (RBM5), also known as LUCA15 or H37, containing two RNA recognition motifs, is a component of the spliceosome A complex. Previously, it has been reported that RBM5 bound to a U/C-rich sequence upstream of the In100 element at intron 9 of caspase2 pre-mRNA that enhanced the formation of proapoptotic caspase-2L isoform. In the present study, we solved the solution structure of the RBM5 RRM2 core domain and characterized its unusual binding capability for different RNA sequences. We found that the RBM5 RRM2 could preferentially bind to both CU rich and GA rich sequences with affinity in 10(-5) molar range. Further NMR experiments revealed that the dual RNA molecules could be accommodated on almost the same region of the protein's β-sheet surface and that both the N- and C-terminal regions of the protein were involved in the recognition. Our studies provide evidence for the RBM5 sequence specific interaction with the cis-acting element in pre-mRNA regulating alternative splicing.
authors: Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, People's Republic of China.
authors: Zhenwei
authors: Z
authors: Song
authors: Peiwen
authors: P
authors: Wu
authors: Peng
authors: P
authors: Ji
authors: Jiahai
authors: J
authors: Zhang
authors: Qingguo
authors: Q
authors: Gong
authors: Jihui
authors: J
authors: Wu
authors: Yunyu
authors: Y
authors: Shi
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D012313:RNA / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry*; D025521:Tumor Suppressor Proteins / Q000737:chemistry*
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; C473448:RBM5 protein, human; D012322:RNA Precursors; D016601:RNA-Binding Proteins; D025521:Tumor Suppressor Proteins; D012313:RNA