title: Molecular identification of a case of Paragonimus pseudoheterotremus infection in Thailand.
abstract: Paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus Paragonimus. In Southeast Asia, Paragonimus heterotremus is the only proven causative pathogen. Recently, a new Paragonimus species, P. pseudoheterotremus, was found in Thailand. This species is genetically similar to P. heterotremus and is considered as a sister species. However, infectivity or pathogenicity of P. pseudoheterotremus to humans remains unclear. We report the first confirmed human pulmonary paragonimiasis case caused by P. pseudoheterotremus infection. After polymerase chain reaction/sequencing of the DNA extracted from Paragonimus eggs in the sputum of the patient, partial internal transcribed spacer 2 and cytochrome c oxidase subunit 1 sequences were approximately identical (98-100%) with those of P. pseudoheterotremus. For P. heterotremus, the partial internal transcribed spacer 2 sequence was approximately identical (99-100%), but the partial mitochondrial cytochrome c oxidase subunit 1 sequence showed a similarity of 90-95%.
authors: Department of Parasitology, Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. pewpan@kku.ac.th
authors: Pewpan M
authors: PM
authors: Intapan
authors: Oranuch
authors: O
authors: Sanpool
authors: Tongjit
authors: T
authors: Thanchomnang
authors: Kanokwan
authors: K
authors: Imtawil
authors: Chatlert
authors: C
authors: Pongchaiyakul
authors: Yukifumi
authors: Y
authors: Nawa
authors: Wanchai
authors: W
authors: Maleewong
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D003576:Electron Transport Complex IV / Q000235:genetics; D006801:Humans; D008174:Lung Diseases, Parasitic / Q000175:diagnosis* / Q000469:parasitology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010237:Paragonimiasis / Q000175:diagnosis* / Q000469:parasitology; D010238:Paragonimus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000469:parasitology; D013785:Thailand
chemical_list: D018509:DNA, Helminth; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV