title: Effect of LED light spectra on starvation-induced oxidative stress in the cinnamon clownfish Amphiprion melanopus.
abstract: The present study aimed to test starvation-induced oxidative stress in the cinnamon clownfish Amphiprion melanopus illuminated by light-emitting diodes (LEDs): red (peak at 630 nm), green (peak at 530 nm), and blue (peak at 450 nm) within a visible light. We investigated the oxidative stress induced by starvation for 12 days during illumination with 3 LED light spectra through measuring antioxidant enzyme (superoxide dismutase [SOD] and catalase [CAT]) mRNA expression and activity; CAT western blotting; and measuring lipid peroxidation [LPO]), plasma H(2)O(2), lysozyme, glucose, alanine aminotransferase (AlaAT), aspartate aminotransferase (AspAT), and melatonin levels. In green and blue lights, expression and activity of antioxidant enzyme mRNA were significantly lower than those of other light spectra, results that are in agreement with CAT protein expression level by western blot analysis. Also, in green and blue lights, plasma H(2)O(2), lysozyme, glucose, AlaAT, AspAT, and melatonin levels were significantly lower than those in other light spectra. These results indicate that green and blue LEDs inhibit oxidative stress and enhance immune function in starved cinnamon clownfish.
authors: Division of Marine Environment & BioScience, Korea Maritime University, Busan 606-791, Republic of Korea. choic@hhu.ac.kr
authors: Cheol Young
authors: CY
authors: Choi
authors: Hyun Suk
authors: HS
authors: Shin
authors: Young Jae
authors: YJ
authors: Choi
authors: Na Na
authors: NN
authors: Kim
authors: Jehee
authors: J
authors: Lee
authors: Gyung-Suk
authors: GS
authors: Kil
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001786:Blood Glucose; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000097:blood; D008027:Light; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000097:blood; D009113:Muramidase / Q000378:metabolism; D018384:Oxidative Stress / Q000528:radiation effects*; D010473:Perciformes / Q000276:immunology / Q000378:metabolism* / Q000502:physiology; D013217:Starvation; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D029941:Fish Proteins; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D009113:Muramidase; D008550:Melatonin