title: Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays.
abstract: The potential of aptamers as ligand binding molecule has opened new avenues in the development of biosensors for cancer oncoproteins. In this paper, a label-free detection strategy using signaling aptamer/protein binding complex for platelet-derived growth factor (PDGF-BB) oncoprotein detection is reported. The detection mechanism is based on the release of fluorophore (TOTO intercalating dye) from the target binding aptamer's stem structure when it captures PDGF. Amino-terminated three-dimensional carbon microarrays fabricated by pyrolyzing patterned photoresist were used as a detection platform. The sensor showed near linear relationship between the relative fluorescence difference and protein concentration even in the sub-nanomolar range with an excellent detection limit of 5pmol. This detection strategy is promising in a wide range of applications in the detection of cancer biomarkers and other proteins.
authors: Department of Mechanical and Materials Engineering, Florida International University, United States.
authors: Varun
authors: V
authors: Penmatsa
authors: A Rahim
authors: AR
authors: Ruslinda
authors: Majid
authors: M
authors: Beidaghi
authors: Hiroshi
authors: H
authors: Kawarada
authors: Chunlei
authors: C
authors: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D000077214:Becaplermin; D015374:Biosensing Techniques / Q000295:instrumentation; D002244:Carbon / Q000737:chemistry*; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D040081:Protein Array Analysis / Q000295:instrumentation*; D020574:Proto-Oncogene Proteins c-sis / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D002244:Carbon