title: Adipose-tissue-derived Stem Cells Enhance the Healing of Ischemic Colonic Anastomoses: An Experimental Study in Rats.
abstract: OBJECTIVE
  This experimental study verified the effect of adipose-tissue-derived stem cells (ASCs) on the healing of ischemic colonic anastomoses in rats.
  
  
  METHODS
  ASCs were isolated from the subcutaneous fat tissue of rats and identified as mesenchymal stem cells by identification of different potentials. An animal model of colonic ischemic anastomosis was induced by modifying Nagahata's method. Sixty male Sprague-Dawley rats (10-week-old, 370 ± 50 g) were divided into two groups (n = 30 each): a control group in which the anastomosis was sutured in a single layer with 6-0 polypropylene without any treatment and an ASCtreated group (ASC group) in which the anastomosis was sutured as in the control group, but then ASCs were locally transplanted into the bowel wall around the anastomosis. The rats were sacrificed on postoperative day 7. Healing of the anastomoses was assessed by measuring loss of body weight, wound infection, anastomotic leakage, mortality, adhesion formation, ileus, anastomotic stricture, anastomotic bursting pressure, histopathological features, and microvascular density.
  
  
  RESULTS
  No differences in wound infection, anastomotic leakage, or mortality between the two groups were observed. The ASC group had significantly more favorable anastomotic healing, including less body weight lost, less ileus, and fewer ulcers and strictures, than the control group. ASCs augmented bursting pressure and collagen deposition. The histopathological features were significantly more favorable in the ASC group, and microvascular density was significantly higher than it was in the control group.
  
  
  CONCLUSIONS
  Locally-transplanted ASCs enhanced healing of ischemic colonic anastomoses by increasing angiogenesis. ASCs could be a novel strategy for accelerating healing of colonic ischemic risk anastomoses.
authors: Department of Surgery, Busan Paik Hospital, Inje University College of Medicine, Busan, Korea.
authors: Jong Han
authors: JH
authors: Yoo
authors: Jae Ho
authors: JH
authors: Shin
authors: Min Sung
authors: MS
authors: An
authors: Tae Kwun
authors: TK
authors: Ha
authors: Kwang Hee
authors: KH
authors: Kim
authors: Ki Beom
authors: KB
authors: Bae
authors: Tae Hyeon
authors: TH
authors: Kim
authors: Chang Soo
authors: CS
authors: Choi
authors: Kwan Hee
authors: KH
authors: Hong
authors: Jeong
authors: J
authors: Kim
authors: Soo Jin
authors: SJ
authors: Jung
authors: Sun Hee
authors: SH
authors: Kim
authors: Kuk Hwan
authors: KH
authors: Rho
authors: Jong Tae
authors: JT
authors: Kim
authors: Young Il
authors: YI
authors: Yang