title: Matrix metalloproteinase-2 promotes αvβ3 integrin-mediated adhesion and migration of human melanoma cells by cleaving fibronectin.
abstract: BACKGROUND
  Matrix metalloproteinase-2 (MMP-2) is a key regulator in the migration of tumor cells. αvβ3 integrin has been reported to play a critical role in cell adhesion and regulate the migration of tumor cells by promoting MMP-2 activation. However, little is known about the effects of MMP-2 on αvβ3 integrin activity and αvβ3 integrin-mediated adhesion and migration of tumor cells.
  
  
  RESULTS
  Human melanoma cells were seeded using an agarose drop model and/or subjected to in vitro analysis using immunofluorescence, adhesion, migration and invasion assays to investigate the relationship between active MMP-2 and αvβ3 integrin during the adhesion and migration of the tumor cells. We found that MMP-2 was localized at the leading edge of spreading cells before αvβ3 integrin. αvβ3 integrin-mediated adhesion and migration of the tumor cells were inhibited by a MMP-2 inhibitor. MMP-2 cleaved fibronectin into small fragments, which promoted the adhesion and migration of the tumor cells.
  
  
  CONCLUSIONS
  MMP-2 cleaves fibronectin into small fragments to enhance the adhesion and migration of human melanoma cells mediated by αvβ3 integrin. These results indicate that MMP-2 may guide the direction of the tumor cell migration.
authors: Institute of Genetics and Cytology, Northeast Normal University, Changchun, Jilin, China.
authors: Yang
authors: Y
authors: Jiao
authors: Xue
authors: X
authors: Feng
authors: Yinpeng
authors: Y
authors: Zhan
authors: Ruifei
authors: R
authors: Wang
authors: Sheng
authors: S
authors: Zheng
authors: Wenguang
authors: W
authors: Liu
authors: Xianlu
authors: X
authors: Zeng
mesh_terms: D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness
chemical_list: D005353:Fibronectins; D039302:Integrin alphaVbeta3; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2