title: MLH1 and XRCC1 polymorphisms in Mexican patients with colorectal cancer.
abstract: DNA  repair proteins maintain DNA integrity; polymorphisms in genes coding for these  proteins can increase susceptibility to colorectal cancer (CRC) development. We  analyzed a possible association of MLH1 -93G>A and 655A>G and XRCC1 Arg194Trp and Arg399Gln polymorphisms with CRC in Mexican patients. Genomic  DNA samples were obtained from peripheral blood of 108 individuals with CRC  (study group) at diagnosis and 120 blood donors (control group) from Western  Mexico; both groups were mestizos. The polymorphisms were detected by PCR-RFLP.  Association was estimated by calculating the odds ratio (OR). We found that the MLH1 and XRCC1 polymorphisms were in Hardy- Weinberg equilibrium.  The MLH1 655A>G polymorphism in the 655G allele was associated with a  2-fold increase risk for CRC (OR = 2.04 and 95% confidence interval (95%CI) =  1.12-3.69; P < 0.01), while the MLH1 -93G>A polymorphism allele  was associated with a protective effect (OR = 0.60, 95%CI = 0.40-0.89; P = 0.01  in the -93A allele and OR = 0.32, 95%CI = 0.13-0.79; P = 0.01 in the AA  genotype). The XRCC1 Arg194Trp and Arg399Gln polymorphisms did not show  any significant associations. In conclusion, we found that MLH1 -93G>A  and 655A>G polymorphisms are associated with CRC in Mexican patients.
authors: Instituto de Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
authors: R
authors: R
authors: Muñiz-Mendoza
authors: M L
authors: ML
authors: Ayala-Madrigal
authors: M
authors: M
authors: Partida-Pérez
authors: J
authors: J
authors: Peregrina-Sandoval
authors: E
authors: E
authors: Leal-Ugarte
authors: N
authors: N
authors: Macías-Gómez
authors: V
authors: V
authors: Peralta-Leal
authors: J P
authors: JP
authors: Meza-Espinoza
authors: J M
authors: JM
authors: Moreno-Ortiz
authors: R
authors: R
authors: Ramírez-Ramírez
authors: S
authors: S
authors: Suárez-Villanueva
authors: M
authors: M
authors: Gutiérrez-Angulo
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D008800:Mexico; D008875:Middle Aged; D000070957:MutL Protein Homolog 1; D009687:Nuclear Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D000076105:X-ray Repair Cross Complementing Protein 1; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004268:DNA-Binding Proteins; C501486:MLH1 protein, human; D009687:Nuclear Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; D000070957:MutL Protein Homolog 1