title: Upregulation of miR-20a and miR-106b is involved in the acquisition             of malignancy of pediatric brainstem gliomas.
abstract: Brainstem glioma (BSG) is an entity which commonly occurs in pediatric patients             and carries a dismal prognosis. However, the category of adult BSG has displayed             considerably benign biological behavior, thus providing a unique perspective to             comparatively understand the malignant features of pediatric BSG. microRNAs (miRNAs)             have been associated with cancer development and progression. To identify miRNAs             specifically involved in the acquisition of malignant progression of pediatric             BSG, we analyzed the miRNA expression profiles in orthotopic models which could             simulate the BSG heterogeneity by microarrays. Our research revealed that miR-20a             and miR-106b (known to be closely related) were the two most robust upregulated             miRNAs in pediatric BSG compared to adult subtype. Furthermore, the two types             of human BSG tissue were utilized to verify the microarray data by qRT-PCR and             in situ hybridization. The results indicated good consistency with that of the             microarray method. In conclusion, our studies provide evidence that miR-20a and             miR-106b may serve as putative causative involvement of malignant progression             of pediatric BSG, thereby serving as likely novel targets for constraining the             rapid fatal course of pediatric BSG.
authors: Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing 100093, PR China.
authors: Xuan
authors: X
authors: Wang
authors: Hongwei
authors: H
authors: Zhang
authors: Anling
authors: A
authors: Zhang
authors: Lei
authors: L
authors: Han
authors: Kun
authors: K
authors: Wang
authors: Ran
authors: R
authors: Liu
authors: Shaohua
authors: S
authors: Yang
authors: Peiyu
authors: P
authors: Pu
authors: Changhong
authors: C
authors: Shen
authors: Chunsheng
authors: C
authors: Kang
authors: Chunjiang
authors: C
authors: Yu
mesh_terms: D000328:Adult; D000367:Age Factors; D000818:Animals; D020295:Brain Stem Neoplasms / Q000235:genetics* / Q000473:pathology*; D002648:Child; D004195:Disease Models, Animal; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D011923:Rats, Nude; D015203:Reproducibility of Results; D015854:Up-Regulation
chemical_list: C542541:MIRN106 microRNA, human; C534451:MIRN20a microRNA, human; D035683:MicroRNAs