title: Isolation and phylogenetic analysis of Bartonella species from wild carnivores of the suborder Caniformia in Japan.
abstract: The prevalence of Bartonella species was investigated among wild carnivores of the suborder Caniformia, including 15 Japanese badgers (Meles anakuma), 8 Japanese martens (Martes melampus), 2 Japanese weasels (Mustela itatsi), 1 Siberian weasel (Mustela sibirica), 171 raccoon dogs (Nyctereutes procyonoides), and 977 raccoons (Procyon lotor) in Japan. Bartonella bacteria were isolated from one Japanese badger (6.7%) and from one Japanese marten (12.5%); however, no Bartonella species was found in other representatives of Caniformia. Phylogenetic analysis was based on concatenated sequences of six housekeeping genes (16S rRNA, ftsZ, gltA, groEL, ribC, and rpoB) and sequence of the 16S-23S internal transcribed spacer region. The sequence analysis indicated that the isolate derived from the Japanese badger (strain JB-15) can represent a novel Bartonella species and the isolate from the Japanese marten (strain JM-1) was closely related to Bartonella washoensis. This is the first report on isolation of Bartonella from badger and marten.
authors: Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors: Shingo
authors: S
authors: Sato
authors: Hidenori
authors: H
authors: Kabeya
authors: Tatsuya
authors: T
authors: Miura
authors: Kazuo
authors: K
authors: Suzuki
authors: Ying
authors: Y
authors: Bai
authors: Michael
authors: M
authors: Kosoy
authors: Hiroshi
authors: H
authors: Sentsui
authors: Hiroaki
authors: H
authors: Kariwa
authors: Soichi
authors: S
authors: Maruyama
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000382:microbiology*; D001473:Bartonella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001474:Bartonella Infections / Q000382:microbiology / Q000662:veterinary*; D046028:Canidae / Q000382:microbiology*; D005798:Genes, Bacterial / Q000235:genetics; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny