title: Lipopolysaccharide regulates MMP-9 expression through TLR4/NF-κB signaling             in human arterial smooth muscle cells.
abstract: Matrix metalloproteinases (MMPs) are critical to vascular smooth muscle             cell migration in vivo. The dysregulation of MMPs is involved in the pathogenesis             of abnormal arterial remodeling, aneurysm formation and atherosclerotic plaque             instability. It has been confirmed that lipopolysaccharides (LPS) constitute a             strong risk factor for the development of atherosclerosis. In this study, we aimed             to determine a potential mechanism of LPS on MMP-9 expression in human arterial             smooth muscle cells (HASMCs). RT-PCR analysis was used to detect MMP-9 mRNA expression             and western blot analysis was performed to examine MMP-9 protein expression. An             electrophoretic mobility shift assay was also employed to determine NF-κB binding             activity. Results showed that LPS induced MMP-9 mRNA and protein expression in             HASMCs in a TLR4-dependent manner. Notably, upon blocking the NF-κB binding with             pyrrolidine dithiocarbamate, it was demonstrated that the expression of MMP-9             by LPS occurs through TLR4/NF-κB pathways. It was concluded that LPS induced MMP-9             expression through the TLR4/NF-κB pathway. Thus, the TLR4/NF-κB pathway may be             involved in the pathogenesis of atherosclerosis.
authors: Department of Cardiology, Shanghai First People's Hospital, College of Medicine, Shanghai Jiaotong University, Shanghai, PR China.
authors: Hongli
authors: H
authors: Li
authors: Hao
authors: H
authors: Xu
authors: Baogui
authors: B
authors: Sun
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D008070:Lipopolysaccharides / Q000633:toxicity*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D011759:Pyrrolidines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013859:Thiocarbamates / Q000494:pharmacology; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D008070:Lipopolysaccharides; D016328:NF-kappa B; D011759:Pyrrolidines; D012333:RNA, Messenger; D013859:Thiocarbamates; D051197:Toll-Like Receptor 4; C020972:pyrrolidine dithiocarbamic acid; D020780:Matrix Metalloproteinase 9