title: "Click & seed" approach to the biomimetic modification of material surfaces.
abstract: A simple, versatile, protein-repulsive, substrate-independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow-up modifications with various ligands using a copper-catalyzed alkyne-azide cycloaddition reaction. The desired surface concentration of peptide biomimetic ligands can be controlled by adjusting the peptide concentration in the reaction mixture, then measuring the activity of (125)I-radiolabeled peptides that are immobilized on the substrates. The performance of the prepared substrates is tested in cell cultures with MEF cells and a human ECC line.
authors: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, vvi, Prague 6, Czech Republic. proks@imc.cas.cz
authors: Vladimír
authors: V
authors: Proks
authors: Josef
authors: J
authors: Jaroš
authors: Ognen
authors: O
authors: Pop-Georgievski
authors: Jan
authors: J
authors: Kučka
authors: Stěpán
authors: S
authors: Popelka
authors: Petr
authors: P
authors: Dvořák
authors: Aleš
authors: A
authors: Hampl
authors: František
authors: F
authors: Rypáček
mesh_terms: D032701:Biomimetics; D002478:Cells, Cultured; D003500:Cyclization; D006801:Humans; D013499:Surface Properties