title: Lupenone isolated from Adenophora triphylla var. japonica extract inhibits adipogenic differentiation through the downregulation of PPARγ in 3T3-L1 cells.
abstract: Adenophora triphylla var. japonica (Campanulaceae) is known to have anti-inflammatory and anti-tussive effects. Dysfunction of adipocytes and adipose tissue in obesity is related to various inflammatory cytokines or adipokines. In this study, we investigated whether lupenone isolated from A. triphylla var. japonica extract inhibits adipocyte differentiation and expression of adipogenic marker genes in 3T3-L1 preadipocytes. We demonstrated that lupenone resulted in a significant reduction in lipid accumulation and expression of adipogenic marker genes in a dose-dependent manner. In addition, lupenone decreased the transcriptional activity of peroxisome proliferator-activated receptor γ (PPARγ) induced by troglitazone, and we also demonstrated that lupenone suppressed the PPARγ and CCAAT-enhancer-binding protein α (C/EBPα) protein levels. These findings demonstrated that lupenone isolated from A. triphylla var. japonica extract effectively inhibited adipocyte differentiation through downregulation of related transcription factor, particularly the PPARγ gene.
authors: Natural Products Research Institute, Gyeonggi Institute of Science and Technology Promotion, 864-1 Ieui-dong, Yeoungtong-gu, Suwon-si, Gyeonggi-do, 443-766, Republic of Korea.
authors: Eun-Kyung
authors: EK
authors: Ahn
authors: Joa Sub
authors: JS
authors: Oh
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D029422:Campanulaceae / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects*; D002839:Chromans / Q000494:pharmacology; D015536:Down-Regulation; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D045162:Thiazolidinediones / Q000494:pharmacology; D015533:Transcriptional Activation / Q000187:drug effects; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D000077288:Troglitazone; D000094042:Lupanes
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D002839:Chromans; D047495:PPAR gamma; D010936:Plant Extracts; D045162:Thiazolidinediones; D014315:Triterpenes; D000077288:Troglitazone; C470592:lupenone; D000094042:Lupanes