title: Live cell imaging of lysosomal pH changes with pH responsive ratiometric lanthanide probes.
abstract: Europium and terbium complexes of two structurally related ligands have been evaluated as optical probes to monitor changes in lysosomal pH; calibration using ionophores and fluorescent probes allows monitoring of the time dependence of lysosomal pH change, examining the green/red intensity ratio from internalised Tb-Eu complexes.
authors: Department of Chemistry, Durham University, South Road, Durham DH1 3LE, UK.
authors: David G
authors: DG
authors: Smith
authors: Brian K
authors: BK
authors: McMahon
authors: Robert
authors: R
authors: Pal
authors: David
authors: D
authors: Parker
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D056831:Coordination Complexes / Q000737:chemistry; D006224:Cricetinae; D003412:Cricetulus; D005063:Europium / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D008247:Lysosomes / Q000737:chemistry / Q000378:metabolism*; D061986:MCF-7 Cells; D051379:Mice; D041681:NIH 3T3 Cells; D013725:Terbium / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D005456:Fluorescent Dyes; D028581:Lanthanoid Series Elements; D013725:Terbium; D005063:Europium