title: Antiproliferative activity and apoptosis induction of Eucalyptus Citriodora resin and its major bioactive compound in melanoma B16F10 cells.
abstract: Antiproliferative activity and apoptosis induction of ethyl acetate of  Eucalyptus citriodora  resin (EAEER), and its major bioactive compound in melanoma B16F10 cells were investigated. 6-[1-(p-Hydroxy-phenyl)ethyl]-7-O-methyl aromadendrin (HEMA), a flavanol derivative, was isolated from EAEER and identified on the basis of its mass and NMR spectra. The results from MTT assay showed high antiproliferative effects of EAEER and HEMA on B16F10 cells. Moreover, EAEER- and HEMA-induced cell apoptosis was association with the decrease in the mitochondrial transmembrane potentials (Δψ(m)), increase in Bax/Bcl-2 ratio, and activation of caspase-3. Cells treated with EAEER and HEMA generated intracellular reactive oxygen species (ROS) and nitric oxide (NO), indicating that ROS and RNS play important roles in the induction of apoptosis in B16F10 cells. Taken together, EAEER and its major bioactive compound, HEMA, inhibited the proliferation of B16F10 cells via apoptosis and may be a potential antimelanoma agent.
authors: Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, Jen-Te District, Tainan, Taiwan, ROC.
authors: Pin-Der
authors: PD
authors: Duh
authors: Zong-Tsi
authors: ZT
authors: Chen
authors: Shwu-Woan
authors: SW
authors: Lee
authors: Tsuey-Pin
authors: TP
authors: Lin
authors: Ya-Ting
authors: YT
authors: Wang
authors: Wen-Jye
authors: WJ
authors: Yen
authors: Ling-Feng
authors: LF
authors: Kuo
authors: Heuy-Ling
authors: HL
authors: Chu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005052:Eucalyptus / Q000737:chemistry*; D008546:Melanoma, Experimental / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008689:Methacrylates / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D012116:Resins, Plant / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D008689:Methacrylates; D012116:Resins, Plant; D051028:bcl-2-Associated X Protein; C005044:hydroxyethyl methacrylate; D053148:Caspase 3