title: Amniotic mesenchymal stem cells enhance wound healing in diabetic NOD/SCID mice through high angiogenic and engraftment capabilities.
abstract: Although human amniotic mesenchymal stem cells (AMMs) have been recognised as a promising stem cell resource, their therapeutic potential for wound healing has not been widely investigated. In this study, we evaluated the therapeutic potential of AMMs using a diabetic mouse wound model. Quantitative real-time PCR and ELISA results revealed that the angiogenic factors, IGF-1, EGF and IL-8 were markedly upregulated in AMMs when compared with adipose-derived mesenchymal stem cells (ADMs) and dermal fibroblasts. In vitro scratch wound assays also showed that AMM-derived conditioned media (CM) significantly accelerated wound closure. Diabetic mice were generated using streptozotocin and wounds were created by skin excision, followed by AMM transplantation. AMM transplantation significantly promoted wound healing and increased re-epithelialization and cellularity. Notably, transplanted AMMs exhibited high engraftment rates and expressed keratinocyte-specific proteins and cytokeratin in the wound area, indicating a direct contribution to cutaneous closure. Taken together, these data suggest that AMMs possess considerable therapeutic potential for chronic wounds through the secretion of angiogenic factors and enhanced engraftment/differentiation capabilities.
authors: Regional Clinical Center, Dong-A University Hospital, Busan, South Korea. sungwhk@yahoo.co.kr
authors: Sung-Whan
authors: SW
authors: Kim
authors: Hong-Zhe
authors: HZ
authors: Zhang
authors: Longzhe
authors: L
authors: Guo
authors: Jong-Min
authors: JM
authors: Kim
authors: Moo Hyun
authors: MH
authors: Kim
mesh_terms: D000650:Amnion / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D003094:Collagen / Q000737:chemistry; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005347:Fibroblasts / Q000166:cytology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007633:Keratins / Q000378:metabolism; D007797:Laminin / Q000737:chemistry; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D018919:Neovascularization, Physiologic; D016133:Polymerase Chain Reaction / Q000379:methods; D011509:Proteoglycans / Q000737:chemistry; D013311:Streptozocin; D014945:Wound Healing
chemical_list: D017077:Culture Media, Conditioned; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D013311:Streptozocin; D007633:Keratins; D003094:Collagen