title: The lncRNA Malat1 is dispensable for mouse development but its transcription plays a cis-regulatory role in the adult.
abstract: Genome-wide studies have identified thousands of long noncoding RNAs (lncRNAs) lacking protein-coding capacity. However, most lncRNAs are expressed at a very low level, and in most cases there is no genetic evidence to support their in vivo function. Malat1 (metastasis associated lung adenocarcinoma transcript 1) is among the most abundant and highly conserved lncRNAs, and it exhibits an uncommon 3'-end processing mechanism. In addition, its specific nuclear localization, developmental regulation, and dysregulation in cancer are suggestive of it having a critical biological function. We have characterized a Malat1 loss-of-function genetic model that indicates that Malat1 is not essential for mouse pre- and postnatal development. Furthermore, depletion of Malat1 does not affect global gene expression, splicing factor level and phosphorylation status, or alternative pre-mRNA splicing. However, among a small number of genes that were dysregulated in adult Malat1 knockout mice, many were Malat1 neighboring genes, thus indicating a potential cis-regulatory role of Malat1 gene transcription.
authors: Cold Spring Harbor Laboratory, One Bungtown Road, Cold Spring Harbor, NY 11724, USA.
authors: Bin
authors: B
authors: Zhang
authors: Gayatri
authors: G
authors: Arun
authors: Yuntao S
authors: YS
authors: Mao
authors: Zsolt
authors: Z
authors: Lazar
authors: Gene
authors: G
authors: Hung
authors: Gourab
authors: G
authors: Bhattacharjee
authors: Xiaokun
authors: X
authors: Xiao
authors: Carmen J
authors: CJ
authors: Booth
authors: Jie
authors: J
authors: Wu
authors: Chaolin
authors: C
authors: Zhang
authors: David L
authors: DL
authors: Spector
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics / Q000378:metabolism; D000818:Animals; D005260:Female; D018507:Gene Expression Regulation, Developmental; D048788:Growth and Development / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008954:Models, Biological; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D038621:Regulatory Sequences, Ribonucleic Acid / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: C557040:Malat1 long non-coding RNA, mouse; D062085:RNA, Long Noncoding; D038621:Regulatory Sequences, Ribonucleic Acid