title: Cyanidin reduces preadipocyte differentiation and relative ChREBP expression.
abstract: Adipogenesis is a continuous process even in adult adipose tissue for the presence of preadipocytes that, when subjected to appropriate stimuli can proliferate and differentiate. ChREBP, the essential transcription factor for lipogenesis, is expressed in all tissues, but mainly in lipogenic organs. In this study, we focused on ChREBP expression during preadipocytes differentiation. Since it was found that cyanidin-3 reduces body weight in mice even in the presence of a high-fat diet, by decreasing levels of blood glucose and by improving insulin sensitivity, we studied the effect of this substance on adipogenic differentiation. For this purpose we used preadipocytes obtained from subcutaneous and visceral human adipose explant tissue, characterized and stimulated to differentiate in selective media. On cytofluorimetric analysis these cells showed mesenchymal markers (CD29, CD90, CD44), whereas they were negative for hematopoietic markers (CD45, CD10, CD117,CD31). ChREBP expression levels were quantified by immunoelectron-microscopy and western blotting analysis. In this report we show that ChREBP is expressed in preadipocytes (both nuclear and cytoplasmic compartments); the cytoplasmic level of ChREBP increased by 50 percent on day seven of differentiation into mature adipocytes. Cyanidin reduced differentiation by 20 percent (as evaluated by red oil O staining) and the expression of ChREBP. In addition, cyanidin-treated cells showed abnormal morphology, a square shape with irregular size, probably due to the fact that cyanidin may interfere with the extracellular matrix. These findings suggest that dietary cyanidin, may have inhibitory effects on adipogenesis.
authors: Institute of Molecular Genetics, National Research Council, Chieti, Italy.
authors: A
authors: A
authors: Pompei
authors: E
authors: E
authors: Toniato
authors: P
authors: P
authors: Innocenti
authors: I
authors: I
authors: D Alimonte
authors: C
authors: C
authors: Cellini
authors: D
authors: D
authors: Mattoscio
authors: R
authors: R
authors: Cotellese
authors: D
authors: D
authors: Bosco
authors: R
authors: R
authors: Ciccarelli
authors: V
authors: V
authors: Dadorante
authors: N
authors: N
authors: D Orazio
authors: S
authors: S
authors: Martinotti
authors: I
authors: I
authors: Robuffo
mesh_terms: D017667:Adipocytes / Q000737:chemistry / Q000166:cytology; D050156:Adipogenesis / Q000187:drug effects*; D000872:Anthocyanins / Q000494:pharmacology*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000032:analysis*; D002454:Cell Differentiation / Q000187:drug effects; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology
chemical_list: D000872:Anthocyanins; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C116385:MLXIPL protein, human; C017154:cyanidin