title: Flow injection determination of hydrogen peroxide using catalytic effect of cobalt(II) ion on a dye formation reaction.
abstract: A novel flow injection photometric method was developed for the determination of hydrogen peroxide in rainwater. This method is based on a cobalt(II)-catalyzed oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone (MBTH) with N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline (DAOS) as a modified Trinder's reagent to produce intensely colored dye (λ(max)=530nm) in the presence of hydrogen peroxide at pH 8.4. In this method, 1,2-dihydroxy-3,5-benzenedisulfonic acid (Tiron) acted as an activator for the cobalt(II)-catalyzed reaction and effectively increased the peak height for hydrogen peroxide. The linear calibration graphs were obtained in the hydrogen peroxide concentration range 5×10(-8) to 2.2×10(-6)mol dm(-3) at a sampling rate of 20h(-1). The relative standard deviations for ten determinations of 2.2×10(-6) and 2×10(-7)mol dm(-3) hydrogen peroxide were 1.1% and 3.7%, respectively. The proposed method was successfully applied to the determination of hydrogen peroxide in rainwater samples and the analytical results agreed fairly well with the results obtained by different two reference methods; peroxidase method and hydrogen peroxide electrode method.
authors: Department of Analytical Chemistry, Faculty of Education, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. emkurih@ipc.shizuoka.ac.jp
authors: Makoto
authors: M
authors: Kurihara
authors: Miyuki
authors: M
authors: Muramatsu
authors: Mari
authors: M
authors: Yamada
authors: Naoya
authors: N
authors: Kitamura
mesh_terms: D052160:Benzothiazoles / Q000737:chemistry; D002138:Calibration; D002384:Catalysis; D003035:Cobalt / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000379:methods*; D006835:Hydrazones / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D010084:Oxidation-Reduction; D011136:Polysorbates / Q000737:chemistry; D011891:Rain; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature
chemical_list: D052160:Benzothiazoles; D004396:Coloring Agents; D006835:Hydrazones; D008024:Ligands; D011136:Polysorbates; D013501:Surface-Active Agents; D003035:Cobalt; D006861:Hydrogen Peroxide; C002536:3-methyl-2-benzothiazolone hydrazone