title: Effects of perfluorinated compounds on development of zebrafish embryos.
abstract: Perfluorinated compounds (PFCs) have been widely used in industrial and consumer products and frequently detected in many environmental media. Potential reproductive effects of perfluorooctanesulfonate (PFOS), perfluorooctanoic acid (PFOA) and perfluorononanoic acid (PFNA) have been reported in mice, rats and water birds. PFOS and PFOA were also confirmed developing toxicants towards zebrafish embryos; however, the reported effect concentrations were contradictory. Polyfluorinated alkylated phosphate ester surfactants (including FC807) are precursor of PFOS and PFOA; however, there is no published information about the effects of FC807 and PFNA on zebrafish embryos. Therefore, this study was conducted to determine the effects of these four PFCs on zebrafish embryos. Normal fertilized zebrafish embryos were selected to be exposed to several concentrations of PFOA, PFNA, PFOS or FC807 in 24-well cell culture plates. A digital camera was used to image morphological anomalies of embryos with a stereomicroscope. Embryos were observed through matching up to 96-h post-fertilization (hpf) and rates of survival and abnormalities recorded. PFCs caused lethality in a concentration-dependent manner with potential toxicity in the order of PFOS > FC807 > PFNA > PFOA based on 72-h LC(50). Forty-eight-hour post-fertilization pericardial edema and 72- or 96-hpf spine crooked malformation were all observed. PFOA, PFNA, PFOS and FC807 all caused structural abnormalities using early stages of development of zebrafish. The PFCs all retarded the development of zebrafish embryos. The toxicity of the PFCs was related to the length of the PFC chain and functional groups.
authors: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210046, China.
authors: Xin-Mei
authors: XM
authors: Zheng
authors: Hong-Ling
authors: HL
authors: Liu
authors: Wei
authors: W
authors: Shi
authors: Si
authors: S
authors: Wei
authors: John P
authors: JP
authors: Giesy
authors: Hong-Xia
authors: HX
authors: Yu
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D004305:Dose-Response Relationship, Drug; D005466:Fluorocarbons / Q000008:administration & dosage / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology*
chemical_list: D005466:Fluorocarbons; D014874:Water Pollutants, Chemical