title: Alteration of glucose metabolism in cultured astrocytes after AQP9-small interference RNA application.
abstract: Aquaglyceroporin-9 (AQP9) facilitates diffusion of water and energy substrates such as glycerol and monocarboxylates. AQP9 is present in plasma membrane and mitochondria of astrocytes and catecholaminergic neurons, suggesting that it plays a role in the energetic status of these cells. Using specific small interference RNA directed against AQP9 in astrocyte cultures, we showed that glycerol uptake is decreased which is associated with an increase in glucose uptake and oxidative metabolism. Our results not only confirm the presence of AQP9 in astrocytes but also suggest that changes in AQP9 expression alter glial energy metabolism.
authors: Department of Pediatrics, Loma Linda University, School of Medicine, Loma Linda, CA 92354, USA. jbadaut@llu.edu
authors: Jérôme
authors: J
authors: Badaut
authors: Jean-François
authors: JF
authors: Brunet
authors: Céline
authors: C
authors: Guérin
authors: Luca
authors: L
authors: Regli
authors: Luc
authors: L
authors: Pellerin
mesh_terms: D000818:Animals; D020346:Aquaporins / Q000235:genetics / Q000378:metabolism*; D001253:Astrocytes / Q000378:metabolism*; D015153:Blotting, Western; D002478:Cells, Cultured; D004734:Energy Metabolism / Q000502:physiology*; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: C491638:Aqp9 protein, mouse; D020346:Aquaporins; D034741:RNA, Small Interfering; D005947:Glucose