title: Study on in vitro release and cell response to alendronate sodium-loaded ultrahigh molecular weight polyethylene loaded with alendronate sodium wear particles to treat the particles-induced osteolysis.
abstract: The aim of this study is to investigate in vitro release and cell response to wear particles of ultrahigh molecular weight polyethylene loaded with alendronate sodium (UHMWPE-ALN), a potent bone resorption inhibitor. Wear particles of UHMWPE-ALN with different ALN contents (0.5 wt % or 1.0 wt %) and size ranges (<45 μm or 45-75 μm) were cocultured with macrophages (RAW264.7) and osteoblasts (MC3T3-E1), respectively. The in vitro ALN release was divided into three stages: an initial burst release, subsequent rapid release, and final slow release. The particle size and ALN content of UHMWPE-ALN wear particles affected the in vitro release mainly during initial burst and rapid release. Compared with the control cells, UHMWPE-ALN wear particles stimulated a significant elevation of tumor necrosis factor-alpha (TNF-α) release from macrophages but had no obvious effect on interleukin-6 release. However, this stimulation of TNF-α release could be reduced by ALN released from UHMWPE-ALN wear particles. The wear particle size had stronger effect of on the macrophages compared with the ALN concentration. After coculture with UHMWPE-ALN wear particles, osteoblast proliferation and alkaline phosphatase activities increased moderately with the increase in particle sizes and ALN concentrations. These results suggest that incorporation of ALN in UHMWPE-ALN may be an effective approach to prevent or reduce particles-induced osteolysis.
authors: Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, People's Republic of China. qushuxin@home.swjtu.edu.cn
authors: Shuxin
authors: S
authors: Qu
authors: Yinlong
authors: Y
authors: Bai
authors: Xiaomin
authors: X
authors: Liu
authors: Rong
authors: R
authors: Fu
authors: Ke
authors: K
authors: Duan
authors: Jie
authors: J
authors: Weng
mesh_terms: D019386:Alendronate / Q000494:pharmacology*; D000818:Animals; D018920:Coculture Techniques; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010014:Osteolysis / Q000209:etiology*; D010316:Particle Size; D011095:Polyethylenes / Q000009:adverse effects*; D011475:Prosthesis Failure / Q000009:adverse effects*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011095:Polyethylenes; C111601:ultra-high molecular weight polyethylene; D019386:Alendronate