title: Standardized and automated solid-phase extraction procedures for high-throughput proteomics of body fluids.
abstract: In order to balance the speed of analytical sample preparation procedures with mass spectrometry (MS)-based clinical proteomics the application of high-throughput robotic systems for body fluid workup is essential. In this paper we describe the implementation of various solid-phase extraction (SPE) sample preparation protocols on two different platforms, namely: 1) Magnetic bead-based SPE of peptides and proteins from body fluids on a Hamilton liquid handling workstation; 2) Cartridge-based SPE on a SPARK Symbiosis system. All SPE protocols were optimized for MS-based proteomics and compared with respect to obtained peptide- and protein profiles. Throughput numbers that were achieved in a 24 hour time frame for the sample workup procedures were more than 700 samples for the magnetic bead-based method and over 1000 samples for the cartridge-based method.
authors: Leiden University Medical Center (LUMC), Department of Parasitology, Biomolecular Mass Spectrometry Unit, Albinusdreef 2, 2300 RC, Leiden, The Netherlands.
authors: Marco R
authors: MR
authors: Bladergroen
authors: Rico J E
authors: RJ
authors: Derks
authors: Simone
authors: S
authors: Nicolardi
authors: Bram
authors: B
authors: de Visser
authors: Sander
authors: S
authors: van Berloo
authors: Yuri E M
authors: YE
authors: van der Burgt
authors: André M
authors: AM
authors: Deelder
mesh_terms: D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D010949:Plasma / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods* / Q000592:standards*; D012371:Robotics / Q000295:instrumentation / Q000379:methods
chemical_list: D001798:Blood Proteins