title: Enantioselective analysis of melagatran via an LSPR biosensor integrated with a microfluidic chip.
abstract: The impact of chiral compounds on pharmacological and biological processes is well known. With the increasing need for enantiomerically pure compounds, effective strategies for enantioseparation and chiral discrimination are in great demand. Herein we report a simple but efficient approach for the enantioselective determination of chiral compounds based on a localized surface plasmon resonance (LSPR) biosensor integrated with a microfluidic chip. A glass microfluidic chip with an effective volume of ~0.75 μL was fabricated for this application. Gold nanorods (AuNRs) with an aspect ratio of ~2.6 were self-assembled onto the surface of the inner wall of the chip to serve as LSPR transducers, which would translate the analyte binding events into quantitative concentration information. Human α-thrombin was immobilized onto the AuNR surface for enantioselective sensing of the enantiomers of melagatran. The proposed sensor was found to be highly selective for RS-melagatran, while the binding of its enantiomer, SR-melagatran, to the sensor was inactive. Under optimal conditions, the limit of detection of this sensor for RS-melagatran was found to be 0.9 nM, whereas the presence of 10,000-fold amounts of SR-melagatran did not interfere with the detection. To the best of our knowledge, this is the first demonstration of an LSPR-based enantioselective biosensor.
authors: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Department of Chemistry, Fuzhou University, Fuzhou, 350108, China. guolh@fzu.edu.cn
authors: Longhua
authors: L
authors: Guo
authors: Yuechun
authors: Y
authors: Yin
authors: Rong
authors: R
authors: Huang
authors: Bin
authors: B
authors: Qiu
authors: Zhenyu
authors: Z
authors: Lin
authors: Huang-Hao
authors: HH
authors: Yang
authors: Jianrong
authors: J
authors: Li
authors: Guonan
authors: G
authors: Chen
mesh_terms: D001384:Azetidines / Q000032:analysis* / Q000737:chemistry; D001596:Benzylamines / Q000032:analysis* / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D043942:Nanotubes / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods; D013917:Thrombin / Q000737:chemistry*
chemical_list: D001384:Azetidines; D001596:Benzylamines; D004800:Enzymes, Immobilized; C109573:melagatran; D006046:Gold; D013917:Thrombin