title: Isolation and characterization of 10 microsatellite loci in Cneorum tricoccon (Cneoraceae), a Mediterranean relict plant.
abstract: OBJECTIVE
  The main aim of this study was to isolate and characterize microsatellite loci in Cneorum tricoccon (Cneoraceae), a Mediterranean shrub relict of the early Tertiary, which inhabits western Mediterranean islands and coasts. Microsatellites will be useful for investigating biogeography and landscape genetics across the species distribution range, including current or past gene flow.
  
  
  RESULTS
  Seventeen microsatellite loci were characterized, of which 10 were polymorphic and amplified for a total of 56 alleles in three populations of C. tricoccon. The markers revealed average coefficients of expected heterozygosity (H(e) = 0.425), observed heterozygosity (H(o) = 0.282), and inbreeding coefficient value per population (F(IS) = 0.408).
  
  
  CONCLUSIONS
  These microsatellite primers will potentially be useful in the study of population and landscape genetics, conservation status of isolated populations, island-continental distribution, current or historical movements between populations, and in the investigation of the consequences of dispersal mechanisms of these plants.
authors: Departamento de Biología y Geología, Universidad Rey Juan Carlos, Madrid, Spain.
authors: Alfredo
authors: A
authors: García-Fernández
authors: Ana
authors: A
authors: Lázaro-Nogal
authors: Anna
authors: A
authors: Traveset
authors: Fernando
authors: F
authors: Valladares
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005602:France; D056426:Genetic Loci; D005819:Genetic Markers; D007558:Italy; D008521:Mediterranean Islands; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000145:classification / Q000235:genetics; D011110:Polymorphism, Genetic; D027821:Rutaceae / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013030:Spain; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers