title: Limitations of macroscopic fluorescence imaging for the estimation of tumour growth in an orthotopic glioma mouse model.
abstract: Imaging methods based on light detection           are being increasingly used for the non-invasive assessment           of tumour growth in animal models. In contrast           with bioluminescence imaging, there are no           studies assessing the use of macroscopic fluorescence           imaging for the longitudinal monitoring of tumour           growth in an orthotopic glioma mouse model. Glioma           cells expressing the red-shifted fluorescent protein           mKate2 were orthotopically implanted to NOD-rag           mice and the tumour size estimated by macroscopic           fluorescence imaging was compared to the tumour           volume determined morphometrically. There was no           significant correlation between the data obtained by           non-invasive macroscopic fluorescence imaging and           post mortem morphometry. In addition, the fluorescence           imaging failed to detect a morphometrically           verified difference in tumour volume between animals           with tumours expressing a potential tumour           suppressor gene and controls. The fluorescence signal           was affected by the spatial pattern of tumour           growth and substantially attenuated by the interfering           brain tissue. Our results indicate that the fluorescence           signal emitted by glioma cells reflected not           only the tumour mass, but also its spatial distribution.           Macroscopic planar FLI in an epi-illumination           mode and a conventional source of excitation light therefore appears to be more suitable for semi-quantitative           assessment of the tumour growth especially           in the case of superficially located tumours rather           than for precise volume estimation of the xenografts           located deep within the brain tissue.
authors: Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University in Prague, Czech Republic.
authors: M
authors: M
authors: Hilšer
authors: J
authors: J
authors: Trylčová
authors: P
authors: P
authors: Bušek
authors: A
authors: A
authors: Šedo
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D004195:Disease Models, Animal; D005453:Fluorescence; D005910:Glioma / Q000175:diagnosis*; D006801:Humans; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D061848:Optical Imaging / Q000379:methods*