title: α-Synuclein inhibits intersynaptic vesicle mobility and maintains recycling-pool homeostasis.
abstract: Although the presynaptic protein α-synuclein is a recognized player in neurodegeneration, its precise physiologic function(s) and/or role in human disease remains unclear. An emerging consensus from previous studies in lower-order systems is that α-synuclein interferes with vesicle-trafficking pathways; however putative neuronal correlates are unknown. Here we explore consequences of α-synuclein modulation in cultured mouse hippocampal neurons; coupling α-synuclein overexpression and knock-out model-systems with contemporary imaging paradigms. Our studies reveal an unexpected role of α-synuclein in attenuating the mobility of recycling pool (RP) vesicles between presynaptic boutons--called "superpool" trafficking--and also in maintaining the overall size of RPs at synapses. While an excess of α-synuclein led to smaller RPs and inhibited intersynaptic trafficking, an absence of α-synuclein triggered converse changes with larger RPs and enhanced intersynaptic trafficking. The data collectively suggest a model where α-synuclein maintains RP homeostasis by modulating intersynaptic vesicular dynamics, and provide a putative neuronal correlate of α-synuclein-induced impairments in vesicle-trafficking previously reported in lower-order systems.
authors: Department of Pathology, University of California, San Diego, La Jolla, California 92093, USA.
authors: David
authors: D
authors: Scott
authors: Subhojit
authors: S
authors: Roy
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000378:metabolism; D006706:Homeostasis / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism*; D013572:Synaptic Vesicles / Q000235:genetics / Q000378:metabolism*; D051844:alpha-Synuclein / Q000235:genetics / Q000378:metabolism*
chemical_list: D051844:alpha-Synuclein