title: Effects of Salusin-β on action potential and ionic currents in ventricular myocytes of rats.
abstract: OBJECTIVE
  Salusin-β is a regulatory peptide that exerts negative inotropic effect on ventricular muscle, but its electrophysiological effects on ventricular myocytes are still unknown.
  
  
  METHODS
  Action potential and channel currents such as sodium current (I(N)             (a) ), transient outward potassium current (I(to) ), steady-state potassium current (I(sus) ), sodium-calcium exchange current (I(N)             (aCa) ) and inward rectifier potassium current (I(K)             (1) ) were measured in ventricular myocytes isolated from 12 to 16 weeks rats by whole-cell voltage-clamp techniques.
  
  
  RESULTS
  Salusin-β dose-dependently shortened the duration of action potential in rat ventricular myocytes. Furthermore, salusin-β significantly inhibited I(N)             (aCa)  and increased I(to) , but did not affect I(N)             (a) , I(sus)  and I(K)             (1) .
  
  
  CONCLUSIONS
  These results suggest that the effect of salusin-β on action potential may be partly attributed to a decrease in inward currents and an increase in outward currents.
authors: Department of Pathophysiology, Second Military Medical University, Shanghai, China. renanjing@gmail.com
authors: An-Jing
authors: AJ
authors: Ren
authors: Qing
authors: Q
authors: Liang
authors: Rong
authors: R
authors: Wang
authors: Hai-Yu
authors: HY
authors: Ji
authors: Yi
authors: Y
authors: Wu
authors: Jing-Song
authors: JS
authors: Shi
authors: Li
authors: L
authors: Lin
authors: Wen-Jun
authors: WJ
authors: Yuan
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D006352:Heart Ventricles / Q000166:cytology; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009200:Myocardial Contraction / Q000187:drug effects / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology; D024661:Potassium Channels, Inwardly Rectifying / Q000502:physiology; D051381:Rats; D015222:Sodium Channels / Q000502:physiology; D019831:Sodium-Calcium Exchanger / Q000502:physiology
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D015222:Sodium Channels; D019831:Sodium-Calcium Exchanger; C528274:salusin-beta, rat