title: Instrumentation and software for mass spectrometry imaging--making the most of what you've got.
abstract: Whilst it might be desirable to be able to purchase an up to date mass spectrometry platform and dedicate it to mass spectrometry imaging, this is not the situation initially for many laboratories. There are a variety of methods by which existing mass spectrometers can be upgraded/adapted to perform mass spectrometry imaging using MALDI, DESI or LAESI as the means of generating ions. The focus of this article is on relatively low cost adaptations of existing instrumentation with suggestions made for performance enhancements where appropriate. A brief description of attempts to perform SIMS imaging on quadrupole time of flight mass spectrometers is also given. The required software is described with particular emphasis on freeware packages which can be used to display/enhance data. Requirements for data pre-processing prior or statistical analysis are discussed along with the use of MATLAB® for the analysis itself.
authors: Lysosomal Diseases Research Unit, SA Pathology at Women's and Children's Hospital, 72 King William Road, North Adelaide, South Australia 5006, Australia.
authors: Paul J
authors: PJ
authors: Trim
authors: Novartis Pharma AG, Basle, Switzerland.
authors: Marie-Claude
authors: MC
authors: Djidja
authors: Department of Chemistry, University of Pittsburgh, Eberly Hall, 200 University Drive Pittsburgh, PA 15260, USA.
authors: Tasneem
authors: T
authors: Muharib
authors: Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom.
authors: Laura M
authors: LM
authors: Cole
authors: Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom.
authors: Bryn
authors: B
authors: Flinders
authors: Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom.
authors: Vikki A
authors: VA
authors: Carolan
authors: Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom.
authors: Simona
authors: S
authors: Francese
authors: Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, United Kingdom. Electronic address: m.r.clench@shu.ac.uk.
authors: Malcolm R
authors: MR
authors: Clench
mesh_terms: D002138:Calibration; D003952:Diagnostic Imaging / Q000295:instrumentation* / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D004864:Equipment and Supplies; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D012984:Software; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods / Q000592:standards