title: 1-Aminocyclopropane-1-carboxylic acid (ACC) deaminase-containing rhizobacteria protect Ocimum sanctum plants during waterlogging stress via reduced ethylene generation.
abstract: Ocimum sanctum grown as rain-fed crop, is known to be poorly adapted to waterlogged conditions. Many a times the crop suffers extreme damages because of anoxia and excessive ethylene generation due to waterlogging conditions present under heavy rain. The usefulness of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-containing plant growth promoting rhizobacteria was investigated under waterlogging stress. The comparison of herb yield and stress induced biochemical changes of waterlogged and non-waterlogged plants with and without ACC deaminase-containing microbiological treatments were monitored in this study. Ten plant growth promoting rhizobacteria strains containing ACC-deaminase were isolated and characterized. Four selected isolates Fd2 (Achromobacter xylosoxidans), Bac5 (Serratia ureilytica), Oci9 (Herbaspirillum seropedicae) and Oci13 (Ochrobactrum rhizosphaerae) had the potential to protect Ocimum plants from flood induced damage under waterlogged glass house conditions. Pot experiments were conducted to evaluate the potential of these ACC deaminase-containing selected strains for reducing the yield losses caused by waterlogging conditions. Bacterial treatments protected plants from waterlogging induced detrimental changes like stress ethylene production, reduced chlorophyll concentration, higher lipid peroxidation, proline concentration and reduced foliar nutrient uptake. Fd2 (A. xylosoxidans) induced maximum waterlogging tolerance as treated waterlogged plants recorded maximum growth and herb yield (46.5% higher than uninoculated waterlogged plants) with minimum stress ethylene levels (53% lower ACC concentration as compared to waterlogged plants without bacterial inoculation) whereas under normal non-waterlogged conditions O. rhizosphaerae was most effective in plant growth promotion.
authors: Microbial Technology Division, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow 226015, Uttar Pradesh, India.
authors: Deepti
authors: D
authors: Barnawal
authors: Nidhi
authors: N
authors: Bharti
authors: Deepamala
authors: D
authors: Maji
authors: Chandan Singh
authors: CS
authors: Chanotiya
authors: Alok
authors: A
authors: Kalra
mesh_terms: D000222:Adaptation, Physiological; D000598:Amino Acids, Cyclic / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology*; D019755:Carbon-Carbon Lyases / Q000378:metabolism*; D005030:Ethylenes / Q000096:biosynthesis*; D055868:Floods; D000860:Hypoxia; D031339:Ocimum / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology; D010100:Oxygen / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D011891:Rain; D012988:Soil Microbiology; D013312:Stress, Physiological; D014867:Water
chemical_list: D000598:Amino Acids, Cyclic; D005030:Ethylenes; D010940:Plant Proteins; D014867:Water; C023863:1-aminocyclopropane-1-carboxylic acid; C036216:ethylene; C033634:1-aminocyclopropane-1-carboxylate deaminase; D019755:Carbon-Carbon Lyases; D010100:Oxygen