title: Analysis of 5'-mononucleotides in infant formula and adult/pediatric nutritional formula by liquid chromatography: First Action 2011.20.
abstract: A method for the routine determination of 5'-mononucleotides (uridine 5'-monophosphate, inosine 5'-monophosphate, adenosine 5'-monophosphate, guanosine 5'-monophosphate, and cytidine 5'-monophosphate) in infant formula and adult nutritionals is described. After sample dissolution and addition of internal standard, potential interferences were removed by anion-exchange SPE followed by HPLC-UV analysis. Single-laboratory validation performance parameters include recovery (92-101%) and repeatability (1.0-2.3% RSD). The method was approved for Official First Action status by an AOAC expert review panel.
authors: Fonterra Co-operative Group Ltd, New Zealand. Brendon.Gill@fonterra.com
authors: Brendon D
authors: BD
authors: Gill
authors: Harvey E
authors: HE
authors: Indyk
authors: Maureen C
authors: MC
authors: Kumar
authors: Nathan K
authors: NK
authors: Sievwright
authors: Merilyn
authors: M
authors: Manley-Harris
authors: Dawn
authors: D
authors: Dowell
mesh_terms: D000249:Adenosine Monophosphate / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003568:Cytidine Monophosphate / Q000032:analysis; D006157:Guanosine Monophosphate / Q000032:analysis; D041943:Infant Formula / Q000737:chemistry*; D007291:Inosine Monophosphate / Q000032:analysis; D011685:Purine Nucleotides / Q000032:analysis*; D011742:Pyrimidine Nucleotides / Q000032:analysis*; D014542:Uridine Monophosphate / Q000032:analysis
chemical_list: D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D007291:Inosine Monophosphate; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D014542:Uridine Monophosphate; D003568:Cytidine Monophosphate