title: Structural mass spectrometry in biologics discovery: advances and future trends.
abstract: Mass spectrometry (MS) is one of the key techniques in protein characterization. In this article, the workflow for MS-based structural characterization of biologics in biopharmaceutical drug discovery is presented, including characterization of primary and higher order structures. Advances in MS techniques in protein characterization are illustrated, including electron transfer dissociation MS (ETD-MS) for primary structure analysis and hydrogen/deuterium exchange MS (HDX-MS) for probing protein higher order structures and mapping epitopes. Future trends in applications of MS to evaluate and optimize candidate molecules in biologics stability studies is also described.
authors: Bioanalytical and Discovery Analytical Sciences, Research and Development, Bristol-Myers Squibb Company, Princeton, NJ 08543, USA. Jingjie.mo@bms.com
authors: Jingjie
authors: J
authors: Mo
authors: Adrienne A
authors: AA
authors: Tymiak
authors: Guodong
authors: G
authors: Chen
mesh_terms: D001688:Biological Products / Q000737:chemistry*; D001702:Biopharmaceutics / Q000379:methods / Q000639:trends; D055808:Drug Discovery / Q000379:methods / Q000639:trends; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011487:Protein Conformation; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry*
chemical_list: D001688:Biological Products; D011506:Proteins