title: [Effect of total flavonoids of Scutellaria baicalensis Georgi on expression of influenza A virus nucleoprotein in HeLa cells].
abstract: OBJECTIVE
  To investigate the effect of total flavonoids of Scutellaria baicalensis Georgi (TFSB) on exogenous expression of influenza A virus nucleoprotein (NP) in HeLa cells.
  
  
  METHODS
  HeLa cells were transiently transfected with the empty vector pcDNA3.1(+) or pcDNA3.1(+)/NP vector harboring influenza A virus NP. The pcDNA3.1(+)/NP-transfected cells were treated with TSFB and the expression of influenza A virus NP in the cell supernatant was measured using colloidal gold immunochromatography 48 h after the transfection; fluorescent quantitative RT-PCR was performed to measure the starting copy number of NP gene.
  
  
  RESULTS
  The cells transfected with pcDNA3.1 (+)/NP with and without TFSB treatment were positive for NP expression. Fluorescent quantitative RT-PCR showed that the starting copy number of NP gene in pcDNA3.1(+)/NP-transfected cells was (8.90±2.53)×10⁶ copies/µl, showing no significant difference from that of (6.15±1.49)×10⁶ copies/µl in pcDNA3.1(+)/NP-transfected cells with subsequent TFSB treatment (P>0.05).
  
  
  CONCLUSIONS
  TFSB treatment does not obviously affect exogenous influenza A virus NP gene expression or its protein synthesis in HeLa cells.
authors: Fourth Affiliated Hospital of Nanchang University, China. qqzqzqz@yahoo.com.cn
authors: Qing
authors: Q
authors: Zhang
authors: Bin
authors: B
authors: Yang
authors: Nongrong
authors: N
authors: Wang
authors: Linjian
authors: L
authors: Duan
authors: Shiqin
authors: S
authors: He
authors: Jian
authors: J
authors: Sun
mesh_terms: D005419:Flavonoids / Q000494:pharmacology*; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics*; D031364:Scutellaria baicalensis; D014162:Transfection; D014758:Viral Core Proteins / Q000096:biosynthesis* / Q000235:genetics*
chemical_list: D005419:Flavonoids; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins