title: Detergent-free incorporation of a seven-transmembrane receptor protein into nanosized bilayer Lipodisq particles for functional and biophysical studies.
abstract: SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, the structural and dynamic integrity of bR was retained when compared with data for bR obtained in the native membrane and in detergents and then with crystal data. This indicates the potential of Lipodisq nanoparticles as a useful membrane mimetic.
authors: Department of Biochemistry, Biomembrane Structure Unit, University of Oxford, Oxford, OX1 3QU, United Kingdom.
authors: Marcella
authors: M
authors: Orwick-Rydmark
authors: Janet E
authors: JE
authors: Lovett
authors: Andrea
authors: A
authors: Graziadei
authors: Ljubica
authors: L
authors: Lindholm
authors: Matthew R
authors: MR
authors: Hicks
authors: Anthony
authors: A
authors: Watts
mesh_terms: D001436:Bacteriorhodopsins / Q000737:chemistry*; D040761:Biomimetic Materials / Q000138:chemical synthesis*; D003460:Crystallization / Q000379:methods*; D003902:Detergents / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D003902:Detergents; D008055:Lipids; D046911:Macromolecular Substances; D001436:Bacteriorhodopsins