title: Optimization of elicitation conditions with methyl jasmonate and salicylic acid to improve the productivity of withanolides in the adventitious root culture of Withania somnifera (L.) Dunal.
abstract: Adventitious root cultures derived from leaf derived callus of Withania somnifera (L.) Dunal were treated with methyl jasmonate and salicylic acid independently. Biomass accumulation, culture age, elicitation period, and culture duration were optimized for higher withanolides production in the two best-responding varieties collected from Kolli hills (Eastern Ghats) and Cumbum (Western Ghats) of Tamil Nadu, India. Between the two elicitors, salicylic acid (SA) improved the production of major withanolides (withanolide A, withanolide B, withaferin A, and withanone) as well as minor constituents (12-deoxy withastramonolide, withanoside V, and withanoside IV) in the Kolli hills variety. Treatment of root biomass (11.70 g FW) on 30-day-old adventitious root cultures with 150 μM SA for 4 h elicitor exposure period resulted in the production of 64.65 mg g(-l) dry weight (DW) withanolide A (48-fold), 33.74 mg g(-l) DW withanolide B (29-fold), 17.47 mg g(-l) DW withaferin A (20-fold), 42.88 mg g(-l) DW withanone (37-fold), 5.34 mg g(-l) DW 12-deoxy withastramonolide (nine fold), 7.23 mg g(-l) DW withanoside V (seven fold), and 9.45 mg g(-l) DW withanoside IV (nine fold) after 10 days of elicitation (40th day of culture) when compared to untreated cultures. This is the first report on the use of elicitation strategy on the significant improvement in withanolides production in the adventitious root cultures of W. somnifera.
authors: Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620-024 Tamil Nadu, India.
authors: Ganeshan
authors: G
authors: Sivanandhan
authors: Muthukrishnan
authors: M
authors: Arun
authors: Subramanian
authors: S
authors: Mayavan
authors: Manoharan
authors: M
authors: Rajesh
authors: Murugaraj
authors: M
authors: Jeyaraj
authors: Gnanajothi Kapil
authors: GK
authors: Dev
authors: Markandan
authors: M
authors: Manickavasagam
authors: Natesan
authors: N
authors: Selvaraj
authors: Andy
authors: A
authors: Ganapathi
mesh_terms: D000085:Acetates / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D003517:Cyclopentanes / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism*; D032341:Withania / Q000254:growth & development / Q000378:metabolism*; D054358:Withanolides / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D054358:Withanolides; C072239:methyl jasmonate; D020156:Salicylic Acid