title: Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag.
abstract: The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.
authors: Department of Chemistry, Columbia University, 3000 Broadway and 119th Street, New York, NY, USA.
authors: Evangelos
authors: E
authors: Gatzogiannis
authors: Zhixing
authors: Z
authors: Chen
authors: Lu
authors: L
authors: Wei
authors: Richard
authors: R
authors: Wombacher
authors: Ya-Ting
authors: YT
authors: Kao
authors: Grygorii
authors: G
authors: Yefremov
authors: Virginia W
authors: VW
authors: Cornish
authors: Wei
authors: W
authors: Min
mesh_terms: D002232:Carbocyanines / Q000032:analysis / Q000378:metabolism; D002470:Cell Survival; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism*; D057809:HEK293 Cells; D006657:Histones / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D013762:Tetrahydrofolate Dehydrogenase / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014295:Trimethoprim / Q000032:analysis / Q000378:metabolism*; D014783:Viscosity
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D006657:Histones; D011993:Recombinant Fusion Proteins; C089187:cyanine dye 3; D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase