title: Evaluation of the Standard Diagnostics Leptospira IgM ELISA for diagnosis of acute leptospirosis in Lao PDR.
abstract: The diagnostic utility of the Standard Diagnostics Leptospira IgM ELISA for detection of acute leptospirosis was assessed in febrile adults admitted in Vientiane, Laos. Using the cut-off suggested by the manufacturer [optical density (OD) ≥0.75], the assay demonstrated limited diagnostic capacity with a sensitivity of 95% and a specificity of 41% compared with the Leptospira microscopic agglutination test, which is the serological gold standard. However, re-evaluation of the diagnostic cut-off to an OD of 1.7 demonstrated improved diagnostic accuracy overall (sensitivity 70%; specificity 78%).
authors: Mahidol-Oxford Tropical Medicine Research Unit (MORU), Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand.
authors: Ampai
authors: A
authors: Tanganuchitcharnchai
authors: Lee
authors: L
authors: Smythe
authors: Michael
authors: M
authors: Dohnt
authors: Rudy
authors: R
authors: Hartskeerl
authors: Manivanh
authors: M
authors: Vongsouvath
authors: Viengmone
authors: V
authors: Davong
authors: Olay
authors: O
authors: Lattana
authors: Paul N
authors: PN
authors: Newton
authors: Stuart D
authors: SD
authors: Blacksell
mesh_terms: D000372:Agglutination Tests; D000907:Antibodies, Bacterial; D004797:Enzyme-Linked Immunosorbent Assay; D005334:Fever / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D007075:Immunoglobulin M; D007812:Laos / Q000453:epidemiology; D007919:Leptospira / Q000276:immunology / Q000302:isolation & purification*; D007922:Leptospirosis / Q000175:diagnosis* / Q000453:epidemiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D007075:Immunoglobulin M