title: Microsatellite markers for Saussurea gnaphalodes (Asteraceae), a native Himalayan mountain species.
abstract: OBJECTIVE
  Microsatellite markers were developed in Saussurea gnaphalodes to investigate its genetic variation and population structure.
  
  
  RESULTS
  Using the combined biotin capture method, 46 microsatellite primer sets were isolated and characterized across 48 S. gnaphalodes individuals from three Tibetan populations. Seventeen of these markers showed polymorphism, and the number of alleles ranged from two to 17. The observed and expected heterozygosities per population ranged from 0 to 0.938 and from 0 to 0.905, respectively.
  
  
  CONCLUSIONS
  These markers provide a useful tool to investigate the genetic diversity and population structure of S. gnaphalodes, and to facilitate further studies on conservation and utilization.
authors: Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.
authors: Liyan
authors: L
authors: Zeng
authors: Lhag
authors: L
authors: Chong
authors: Tashi
authors: T
authors: Tersing
authors: Nor
authors: N
authors: Bu
authors: Jiayuan
authors: J
authors: Zhao
authors: Yang
authors: Y
authors: Zhong
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D056426:Genetic Loci; D005819:Genetic Markers; D014644:Genetic Variation; D005828:Genetics, Population; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000145:classification / Q000235:genetics; D011110:Polymorphism, Genetic; D030441:Saussurea / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers