title: Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources.
abstract: We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901-902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than conventional systems. Twenty-three cellulosomal proteins were, without purification, identified by direct analysis of the culture medium. Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of carbon source-adapted cellulosome components.
authors: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, 606-8502, Japan. miueda@kais.kyoto-u.ac.jp.
authors: Hironobu
authors: H
authors: Morisaka
authors: Kazuma
authors: K
authors: Matsui
authors: Yohei
authors: Y
authors: Tatsukami
authors: Kouichi
authors: K
authors: Kuroda
authors: Hideo
authors: H
authors: Miyake
authors: Yutaka
authors: Y
authors: Tamaru
authors: Mitsuyoshi
authors: M
authors: Ueda