title: Expression of p24 gag protein of bovine leukemia virus in insect cells and its use in immunodetection of the disease.
abstract: Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
authors: University of La Plata, La Plata, Argentina.
authors: Alejandra
authors: A
authors: Larsen
authors: Ester Teresa
authors: ET
authors: Gonzalez
authors: María Soledad
authors: MS
authors: Serena
authors: María Gabriela
authors: MG
authors: Echeverría
authors: Eduardo
authors: E
authors: Mortola
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D016583:Enzootic Bovine Leukosis / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D007313:Insecta / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D011994:Recombinant Proteins; D014764:Viral Proteins