title: Coordinated and sequential transcription of the cyprinid herpesvirus-3 annotated genes.
abstract: Cyprinid herpesvirus-3 (CyHV-3) is the cause of a fatal disease in carp and koi fish. The disease is seasonal and appears when water temperatures range from 18 to 28°C. CyHV-3 is a member of the Alloherpesviridae, a family in the Herpesvirales order that encompasses mammalian, avian and reptilian viruses. CyHV-3 is a large double-stranded DNA (dsDNA) herpesvirus with a genome of approximately 295kbp, divergent from other mammalian, avian and reptilian herpesviruses, but bearing several genes similar to cyprinid herpesvirus-1 (CyHV-1), CyHV-2, anguillid herpesvirus-1 (AngHV-1), ictalurid herpesvirus-1 (IcHV-1) and ranid herpes virus-1 (RaHV-1). Here we show that viral DNA synthesis commences 4-8h post-infection (p.i.), and is completely inhibited by pre-treatment with cytosine β-d-arabinofuranoside (Ara-C). Transcription of CyHV-3 genes initiates after infection as early as 1-2h p.i., and precedes viral DNA synthesis. All 156 annotated open reading frames (ORFs) of the CyHV-3 genome are transcribed into RNAs, most of which can be classified into immediate early (IE or α), early (E or β) and late (L or γ) classes, similar to all other herpesviruses. Several ORFs belonging to these groups are clustered along the viral genome.
authors: Department of Pathology and the Lautenberg Center for General and Tumor Immunology, the Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
authors: Maya
authors: M
authors: Ilouze
authors: Arnon
authors: A
authors: Dishon
authors: Moshe
authors: M
authors: Kotler
mesh_terms: D000818:Animals; D002347:Carps; D002478:Cells, Cultured; D005347:Fibroblasts / Q000821:virology; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D013997:Time Factors; D014158:Transcription, Genetic