title: Effects of griseofulvin on in vitro porcine oocyte maturation and embryo development.
abstract: Griseofulvin is an orally administered antifungal drug that affects microtubule formation in vitro and interferes with microtubule dynamics in vivo as clearly shown for mitotic cells in several cell systems. This article reports the effects of griseofulvin on in vitro maturation of porcine oocytes and subsequent effects on embryo development. Our results revealed a concentration-dependent effect on meiotic spindles with 20-40 μM griseofulvin affecting oocyte maturation, and 40 μM affecting fertilization and embryo development. These concentrations of griseofulvin did not affect mitochondrial and cortical granule distribution that also depend on microtubule and cytoskeletal functions during oocyte maturation. Specific effects on the meiotic spindle included spindle disorganization and aberrant chromosome separation displayed as prominent chromosome clusters in oocytes treated with 40 μM griseofulvin. These results strongly suggested that griseofulvin affected porcine oocyte in vitro maturation and following embryo development by disturbing microtubule dynamics.
authors: Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri 65211, USA.
authors: Yi-Liang
authors: YL
authors: Miao
authors: Xia
authors: X
authors: Zhang
authors: Jian-Guo
authors: JG
authors: Zhao
authors: Lee
authors: L
authors: Spate
authors: Ming-Tao
authors: MT
authors: Zhao
authors: Clifton N
authors: CN
authors: Murphy
authors: Randall S
authors: RS
authors: Prather
authors: Qing-Yuan
authors: QY
authors: Sun
authors: Heide
authors: H
authors: Schatten
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D005307:Fertilization in Vitro / Q000187:drug effects; D006118:Griseofulvin / Q000494:pharmacology*; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D008870:Microtubules / Q000187:drug effects; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D008941:Spindle Apparatus / Q000187:drug effects*; D034421:Sus scrofa
chemical_list: D000935:Antifungal Agents; D006118:Griseofulvin