title: Cationic polyelectrolyte copolymer modified polyurethane foam for flow injection preconcentration and separation of trace amounts of β-lactam antibiotics.
abstract: A more sensitive flow injection preconcentration method has been developed for the determination of four β-lactam antibiotics (BLAs) namely cefaclor, cefotaxime, amoxicillin and ampicillin in urine, pharmaceuticals and milk. A mini-column packed with PUF functionalized with the cationic polyelectrolyte, poly(N-chloranil N,N,N',N'-tetramethylethylene diammonium dichloride) PCTDD, was utilized for selective preconcentration. The detection limits with this method were 3.3, 3.8, 5.1 and 7.0 ng mL(-1) and enrichment factors were 38, 21, 39, and 36 for cefaclor, cefotaxime, amoxicillin and ampicillin, respectively with a sample throughput of 12 h(-1) for all BLAs. Moreover, the BLAs were successfully separated by isocratic elution using a micellar mobile phase. Application of the method developed has resulted in recovery values in the range 95%-109% (RSD≤8.7), 83%-99% (RSD≤9.7) and 91%-103% (RSD≤4.0) for urine, pharmaceuticals and milk samples, respectively.
authors: Chemistry Department, Faculty of Science, Fayoum University, Fayoum City, Egypt. samymoh2000@yahoo.com
authors: S M
authors: SM
authors: Abdel Azeem
authors: H M
authors: HM
authors: Kuss
authors: M F
authors: MF
authors: El-Shahat
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000652:urine; D002713:Chlorine / Q000737:chemistry; D017022:Flow Injection Analysis / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides / Q000737:chemistry; D008892:Milk / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D047090:beta-Lactams / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification* / Q000652:urine
chemical_list: D000900:Anti-Bacterial Agents; D006878:Hydroxides; D011108:Polymers; D011140:Polyurethanes; D047090:beta-Lactams; D002713:Chlorine; C031356:hydroxide ion