title: Acute phase proteins: a potential approach for diagnosing chronic infection by Trypanosoma vivax.
abstract: The present study aimed to assess potential changes in acute phase proteins in sheep experimentally infected with Trypanosoma vivax. There were studied eight male sheep, four used as controls and four infected with 10(5) T. vivax trypomastigotes. Blood samples were collected at two points times before infection and then at 5,7, 9, 11, 13, 15, 20, 30, 45, 60, 75, 90, 105 and 120 days post-infection (dpi). Blood samples were centrifuged and allotted, and acute phase proteins were then separated by electrophoresis on acrylamide gel containing sodium dodecyl sulfate. Protein concentrations were determined by computer-assisted densitometry. Total protein was determined by colorimetric biuret method. Trypanosomes were counted daily using a 5 mL aliquot of blood smear on a glass slide under a 22 × 22 mm coverslip. Parasites were counted in 100 microscopic fields (40× magnification), and then multiplied by a correction factor. The results were expressed as parasites per mL of blood. For statistical analyses, we used the Wilcoxon test at 5% significance level. There was found a reduction in several acute phase proteins and increase in antitrypsin and transferrin. This finding can be used for the diagnosis of T. vivax infection, especially in chronic infection.
authors: Laboratório de Higiene e Saúde Pública, Escola de Medicina Veterinária e Zootecnia, Universidade Federal do Tocantins, Araguaína, TO, Brazil. katyanesalmeida@gmail.com
authors: Katyane de Sousa
authors: Kde S
authors: Almeida
authors: Alinny Ferreira
authors: AF
authors: Costa
authors: Paulo Cesar da
authors: PC
authors: Silva
authors: José Jurandir
authors: JJ
authors: Fagliari
authors: Rosangela Zacarias
authors: RZ
authors: Machado
authors: Adjair Antonio do
authors: AA
authors: Nascimento
mesh_terms: D000209:Acute-Phase Proteins / Q000032:analysis*; D000818:Animals; D002908:Chronic Disease; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood* / Q000175:diagnosis*; D016689:Trypanosoma vivax; D014352:Trypanosomiasis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D000209:Acute-Phase Proteins