title: Establishment and characterization of a new human gallbladder carcinoma cell line.
abstract: BACKGROUND
  Prognosis for patients with gallbladder carcinoma (GBC) is poor and the standard treatment for GBC has not yet been established.
  
  
  METHODS
  We established the human GBC cell line TYGBK-1, from a patient with papillary, tubular adenocarcinoma.
  
  
  RESULTS
  The doubling time was 48 hours. This cell line has a missense mutation of p53 and no mutation of the K-RAS gene. This cell line was transplantable to nude mice. We characterized the sensitivity of TYGBK-1 to gemcitabine. We also examined the association of two gemcitabine-related genes (deoxycytidine kinase, dCK, and Hu antigen R, HuR). Among four GBC cell lines (TYGBK-1, NOZ, G-415, TGBC2TKB), TYGBK-1 and NOZ exhibited sensitivity to gemcitabine. Furthermore, these cells expressed both dCK and HuR mRNA, rather than gemcitabine-resistant cells.
  
  
  CONCLUSIONS
  The newly established GBC cell line TYGBK-1, may represent an effective tool for development of chemotherapeutic treatment for GBC.
authors: Department of Surgery and Science, University of Toyama, 2630 Sugitani, Toyama-city, Toyama, 930-0194, Japan. sekky@med.u-toyama.ac.jp
authors: Shinichi
authors: S
authors: Sekine
authors: Yutaka
authors: Y
authors: Shimada
authors: Takuya
authors: T
authors: Nagata
authors: Makoto
authors: M
authors: Moriyama
authors: Testuya
authors: T
authors: Omura
authors: Isaku
authors: I
authors: Yoshioka
authors: Ryouta
authors: R
authors: Hori
authors: Koushi
authors: K
authors: Matsui
authors: Shigeaki
authors: S
authors: Sawada
authors: Tomoyuki
authors: T
authors: Okumura
authors: Toru
authors: T
authors: Yoshida
authors: Kazuhiro
authors: K
authors: Tsukada
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D000231:Adenocarcinoma, Papillary / Q000235:genetics / Q000473:pathology; D000368:Aged; D000818:Animals; D001483:Base Sequence; D045744:Cell Line, Tumor; D017931:DNA Primers; D005260:Female; D005706:Gallbladder Neoplasms / Q000235:genetics / Q000473:pathology*; D016158:Genes, p53; D011905:Genes, ras; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D020125:Mutation, Missense; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers