title: Optical nanobiosensor: a new analytical tool for monitoring carboplatin-DNA interaction in vitro.
abstract: The interaction of DNA and Carboplatin was studied with DNA labeled gold nanoparticles (AuNPs) based optical nanobiosensor. Carboplatin, a cytotoxic drug, is responsible for producing nephrotoxicity at effective dose. Thus, we have developed optical nanobiosensor for monitoring carboplatin-DNA interaction based on Fluorescence Resonance Energy Transfer (FRET) phenomenon. Paracetamol, an analgesic agent, was used as controlled drug in this study. The DNA labeled AuNPs, exposed to carboplatin, a binding event among the DNA and carboplatin takes place, resulting in a conformational change within the biosensor complex which decreases the distance among the fluorescent molecules or the fluorescent/quencher molecules. As the carboplatin interact with DNA, an increase in fluorescence intensity was observed. So, the major difference in increased fluorescence intensity between carboplatin-DNA and paracetamol-DNA interaction shows significant observations. Results have demonstrated that Optical sensor is able to rapidly and effectively monitor carboplatin-DNA interaction with a detection limit up to 0.45 μg/ml. This suggests that the developed optical nanobiosensor was ideal for monitoring Drug-DNA interaction studies while performing combinatorial synthesis for new drug development.
authors: Institute of Research and Development, Gujarat Forensic Sciences University, Near Police Bhavan, Sector 18A, Gandhinagar, India. lad_amit2@yahoo.co.in
authors: Amitkumar N
authors: AN
authors: Lad
authors: Y K
authors: YK
authors: Agrawal
mesh_terms: D000082:Acetaminophen / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D016190:Carboplatin / Q000378:metabolism*; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D055620:Optical Phenomena
chemical_list: D000970:Antineoplastic Agents; D000082:Acetaminophen; D006046:Gold; D004247:DNA; D016190:Carboplatin