title: Effect of ANXA2 gene single nucleotide polymorphism (SNP) on the development of osteonecrosis in Indian sickle cell patient: a PCR-RFLP approach.
abstract: Osteonecrosis is a serious complication in sickle cell patients. The common sites of the necrosis are femoral head, head of the humerus and acetabulam. Annexin A2 (ANXA2) protein mainly functions in bone formation and bone resorption. Alteration of ANXA2 gene may affect the manifestations of osteonecrosis in the patients. PCR-RFLP is a common applicable technique for the detection of known mutation/polymorphisms. Here we are presenting application of the PCR-RFLP technique for determination of the ANXA2 gene single nucleotide polymorphism frequency and their clinical association among Indian sickle cell patients. Five known SNPs of ANXA2 gene (rs7170178, rs73435133, rs73418020, rs72746635 and rs73418025) were determined using the HpyCH4V, DdeI, HpyCH4III and Sau 961 restriction enzyme respectively. Restriction enzyme DdeI was common for rs73435133 and rs72746635 SNP. Only the rs7170178 SNP was detected among patient and control and the other four SNPs were absent in the studied groups. The frequency of ANXA2 gene rs7170178 SNP (A/G, G/G) was comparatively higher in sickle cell patients than controls and it was clinically associated with sickle cell osteonecrosis. The P value of heterozygotes (A/G) and homozygotes (G/G) genotypes were <0.001 and 0.001 respectively, which were highly significant. This study established the application of PCR-RFLP in detection of ANXA2 SNPs in sickle cell patients.
authors: Department of Hematology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110 029, India.
authors: Sanjay
authors: S
authors: Pandey
authors: Ravi
authors: R
authors: Ranjan
authors: Sweta
authors: S
authors: Pandey
authors: Rahasya Mani
authors: RM
authors: Mishra
authors: Tulika
authors: T
authors: Seth
authors: Renu
authors: R
authors: Saxena
mesh_terms: D000755:Anemia, Sickle Cell / Q000150:complications / Q000235:genetics*; D017306:Annexin A2 / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D006801:Humans; D007194:India; D010020:Osteonecrosis / Q000150:complications / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: C472822:ANXA2 protein, human; D017306:Annexin A2; D017931:DNA Primers