title: In vitro refolding process of bovine allergen β-lactoglobulin by Multispectroscopic method.
abstract: OBJECTIVE
  To characterize the relationship between the refolding process of recombinant bovine β-lactoglobulin and its immunoreactivity for clinical purposes. To establish a spectral method which examine the extent of recombinant allergen renaturation.
  
  
  METHODS
  The refolding process of recombinant bovine β-lactoglobulin was investigated by using circular dichroism, fluorescence and synchronous fluorescence spectra. IgE-binding capacity of recombinant protein was analyzed by ELISA. In addition, bioinformatic methods were used to explain the spectral characteristics and analyze the relationship between the conformational changes and the immunoreactivity of the protein during renaturation in vitro.
  
  
  RESULTS
  Renaturation of recombinant bovine β-lactoglobulin resulted in a more compact structure resembling the natural counterpart with stronger IgE-binding capacity.
  
  
  CONCLUSIONS
  The degree of protein renaturation correlated with the IgE-binding capacity of the protein. Results from this study may be of help for food allergy therapy and development of vaccination in the future.
authors: College of Light Industry and Food Science, South China University of Technology, Guangzhou 510642, Guangdong, China.
authors: Xu Li
authors: XL
authors: Wu
authors: Wen Pu
authors: WP
authors: Wang
authors: Li Xin
authors: LX
authors: Xia
authors: Hong
authors: H
authors: Xu
authors: Hui
authors: H
authors: Wu
authors: Zhi Gang
authors: ZG
authors: Liu
mesh_terms: D000485:Allergens; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004797:Enzyme-Linked Immunosorbent Assay; D007073:Immunoglobulin E; D007782:Lactoglobulins / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D000485:Allergens; D007782:Lactoglobulins; D007073:Immunoglobulin E