title: The N-terminal TOG domain of Arabidopsis MOR1 modulates affinity for microtubule polymers.
abstract: Microtubule-associated proteins of the highly conserved XMAP215/Dis1 family promote both microtubule growth and shrinkage, and move with the dynamic microtubule ends. The plant homologue, MOR1, is predicted to form a long linear molecule with five N-terminal TOG domains. Within the first (TOG1) domain, the mor1-1 leucine to phenylalanine (L174F) substitution causes temperature-dependent disorganization of microtubule arrays and reduces microtubule growth and shrinkage rates. By expressing the two N-terminal TOG domains (TOG12) of MOR1, both in planta for analysis in living cells and in bacteria for in vitro microtubule-binding and polymerization assays, we determined that the N-terminal domain of MOR1 is crucial for microtubule polymer binding. Tagging TOG12 at the N-terminus interfered with its ability to bind microtubules when stably expressed in Arabidopsis or when transiently overexpressed in leek epidermal cells, and impeded polymerase activity in vitro. In contrast, TOG12 tagged at the C-terminus interacted with microtubules in vivo, rescued the temperature-sensitive mor1-1 phenotype, and promoted microtubule polymerization in vitro. TOG12 constructs containing the L174F mor1-1 point mutation caused microtubule disruption when transiently overexpressed in leek epidermis and increased the affinity of TOG12 for microtubules in vitro. This suggests that the mor1-1 mutant protein makes microtubules less dynamic by binding the microtubule lattice too strongly to support rapid plus-end tracking. We conclude from our results that a balanced microtubule affinity in the N-terminal TOG domain is crucial for the polymerase activity of MOR1.
authors: Department of Botany, University of British Columbia, Vancouver, Canada.
authors: Bettina
authors: B
authors: Lechner
authors: Madeleine C
authors: MC
authors: Rashbrooke
authors: David A
authors: DA
authors: Collings
authors: Ryan C
authors: RC
authors: Eng
authors: Eiko
authors: E
authors: Kawamura
authors: Angela T
authors: AT
authors: Whittington
authors: Geoffrey O
authors: GO
authors: Wasteneys
mesh_terms: D019943:Amino Acid Substitution; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D007930:Leucine / Q000235:genetics; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism / Q000502:physiology; D010649:Phenylalanine / Q000235:genetics; D019441:Plant Epidermis / Q000378:metabolism; D058105:Polymerization; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: D029681:Arabidopsis Proteins; C429183:MOR1 protein, Arabidopsis; D008869:Microtubule-Associated Proteins; D011108:Polymers; D010649:Phenylalanine; D007930:Leucine