title: Identification of Pold2 as a novel interaction partner of protein inhibitor             of activated STAT2.
abstract: Pold2 is a subunit of the DNA polymerase δ complex, encoding a protein involved in DNA replication and repair. In this study, using a yeast two-hybrid screening technique and the common cDNA fragment of the mouse PIAS2 as a bait, Pold2 was found to interact with PIAS2. A direct interaction between Pold2 and PIAS2 was confirmed by direct yeast two-hybrid. In vivo evidence of Pold2 association with PIAS2 was obtained by co-immunoprecipitation using HEK-293 cells. Subcellular localization studies demonstrated that Pold2 and PIAS2 were partially co-localized in mammalian cells. Collectively, our results suggest that Pold2 interacts under physiological conditions with PIAS2.
authors: Department of Histology and Embryology, Medical College, Yangzhou University, Yangzhou 225001, PR China.
authors: Ying
authors: Y
authors: Zheng
authors: Shudong
authors: S
authors: Sheng
authors: Haiyan
authors: H
authors: Wang
authors: Xiaoqing
authors: X
authors: Jia
authors: Yanqiu
authors: Y
authors: Hu
authors: Yayun
authors: Y
authors: Qian
authors: Yongze
authors: Y
authors: Zhu
authors: Jianjun
authors: J
authors: Wang
mesh_terms: D000818:Animals; D004258:DNA Polymerase III / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D050828:Protein Inhibitors of Activated STAT / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D025941:Protein Interaction Mapping; D050795:STAT2 Transcription Factor / Q000378:metabolism; D014162:Transfection; D020798:Two-Hybrid System Techniques
chemical_list: D018076:DNA, Complementary; C581222:Pold2 protein, mouse; D050828:Protein Inhibitors of Activated STAT; D050795:STAT2 Transcription Factor; D004258:DNA Polymerase III