title: MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins.
abstract: Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNA(Glu), however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity.
authors: Western Australian Institute for Medical Research and Centre for Medical Research, The University of Western Australia, Perth, Western Australia 6000, Australia.
authors: Stefan M K
authors: SM
authors: Davies
authors: Maria I G
authors: MI
authors: Lopez Sanchez
authors: Reena
authors: R
authors: Narsai
authors: Anne-Marie J
authors: AM
authors: Shearwood
authors: Muhammad F M
authors: MF
authors: Razif
authors: Ian D
authors: ID
authors: Small
authors: James
authors: J
authors: Whelan
authors: Oliver
authors: O
authors: Rackham
authors: Aleksandra
authors: A
authors: Filipovska
mesh_terms: D045744:Cell Line, Tumor; D003576:Electron Transport Complex IV / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism*; D054679:Ribosome Subunits, Small / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
chemical_list: C577036:MRPS27 protein, human; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D003576:Electron Transport Complex IV