title: Anion inhibition studies of an α-carbonic anhydrase from the thermophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1.
abstract: The newly discovered thermophilic bacterium Sulfurihydrogenibium yellowstonense YO3AOP1 encodes an α-carbonic anhydrases (CAs, EC 4.2.1.1) which is highly catalytically active and thermostable. Here we report the inhibition of this enzyme, denominated SspCA, with inorganic and complex anions and other molecules interacting with zinc proteins. SspCA was inhibited in the micromolar range by diethyldithiocarbamate, sulfamide, sulfamic acid, phenylboronic and phenylarsonic acid, trithiocarbonate and selenocyanide (K(I)s of 4-70 μM) and in the submillimolar one by iodide, cyanide, (thio)cyanate, hydrogen sulfide, azide, nitrate, nitrite, many complex anions incorporating heavy metal ions and iminodisulfonate (K(I)s of 0.48-0.86 mM). SspCA was not substantially inhibited by bicarbonate and carbonate, hydrogensulfite and peroxidisulfate (K(I)s in the range of 21.1-84.6mM). The exceptional thermostability and lack of strong affinity for hydrogensulfide, bicarbonate, and carbonate make this enzyme an interesting candidate for biotechnological applications of enzymatic CO(2) fixation.
authors: Istituto di Biochimica delle Proteine-CNR, Via P. Castellino 111, 80131 Napoli, Italy.
authors: Viviana
authors: V
authors: De Luca
authors: Daniela
authors: D
authors: Vullo
authors: Andrea
authors: A
authors: Scozzafava
authors: Vincenzo
authors: V
authors: Carginale
authors: Mosè
authors: M
authors: Rossi
authors: Claudiu T
authors: CT
authors: Supuran
authors: Clemente
authors: C
authors: Capasso
mesh_terms: D000595:Amino Acid Sequence; D000838:Anions / Q000737:chemistry / Q000494:pharmacology; D002257:Carbonic Anhydrase Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism*; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy; D006091:Gram-Negative Chemolithotrophic Bacteria / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D000838:Anions; D002257:Carbonic Anhydrase Inhibitors; D002256:Carbonic Anhydrases