title: Integrative proteomic profiling of protein activity and interactions using protein arrays.
abstract: Proteomic studies based on abundance, activity, or interactions have been used to investigate protein functions in normal and pathological processes, but their combinatory approach has not been attempted. We present an integrative proteomic profiling method to measure protein activity and interaction using fluorescence-based protein arrays. We used an on-chip assay to simultaneously monitor the transamidating activity and binding affinity of transglutaminase 2 (TG2) for 16 TG2-related proteins. The results of this assay were compared with confidential scores provided by the STRING database to analyze the functional interactions of TG2 with these proteins. We further created a quantitative activity-interaction map of TG2 with these 16 proteins, categorizing them into seven groups based upon TG2 activity and interaction. This integrative proteomic profiling method can be applied to quantitative validation of previously known protein interactions, and in understanding the functions and regulation of target proteins in biological processes of interest.
authors: Department of Molecular and Cellular Biochemistry, Kangwon National University School of Medicine, Kangwon-Do, Korea.
authors: Se-Hui
authors: SH
authors: Jung
authors: Kangseung
authors: K
authors: Lee
authors: Deok-Hoon
authors: DH
authors: Kong
authors: Woo Jin
authors: WJ
authors: Kim
authors: Young-Myeong
authors: YM
authors: Kim
authors: Kwon-Soo
authors: KS
authors: Ha
mesh_terms: D019881:Aminoacyltransferases / Q000378:metabolism; D000818:Animals; D002232:Carbocyanines / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D002232:Carbocyanines; C085321:cyanine dye 5; D019881:Aminoacyltransferases; C023175:transamidases; D000091345:Protein Glutamine gamma Glutamyltransferase 2; D011503:Transglutaminases; D019204:GTP-Binding Proteins