title: Optimization and characterization of a polysaccharide produced by Pseudomonas fluorescens WR-1 and its antioxidant activity.
abstract: The extracellular polysaccharide produced by a newly isolated strain Pseudomonas fluorescens WR-1 was purified and characterized and its production was optimized using response surface methodology. The results showed that the strain WR-1 produced one kind of EPS that was composed of arabinose, glucose and uronic acid. The molecular weight of the EPS was determined to be 6.78 × 10(6)Da. The preferable culture conditions for EPS production were pH 7.0, temperature 28°C for 72 h with peptone and maltose as best N and C sources, respectively. The model predicted that the maximum EPS production (39.6 gL(-1)) was appeared with maltose 48.65 gL(-1), Mn(2+) 1118 μM and Zn(2+) 901 μM. The EPS also showed good H(2)O(2) scavenging activity while moderate free radical scavenging activity and reductive ability were determined. The EPS from WR-1 may be a new source of natural antioxidants with potential value for health, food and industry.
authors: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors: Waseem
authors: W
authors: Raza
authors: Wang
authors: W
authors: Yang
authors: Yuan
authors: Y
authors: Jun
authors: Faheem
authors: F
authors: Shakoor
authors: Qiwei
authors: Q
authors: Huang
authors: Qirong
authors: Q
authors: Shen
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002138:Calibration; D005591:Chemical Fractionation; D004345:Drug Industry / Q000379:methods / Q000592:standards; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008970:Molecular Weight; D010084:Oxidation-Reduction / Q000187:drug effects; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011551:Pseudomonas fluorescens / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D011135:Polysaccharides, Bacterial; D006861:Hydrogen Peroxide