title: Immobilization of glucansucrase for the production of gluco-oligosaccharides from Leuconostoc mesenteroides.
abstract: Glucansucrase from Leuconostoc mesenteroides was immobilized in 1 % (w/v) with sodium alginate to produce oligosaccharides. Glucansucrase gave three activity bands of approx. 240, 178, and 165 kDa after periodic acid-Schiff staining with sucrose. The immobilized enzyme had 40 % activity after ten batch reactions at 30 °C and 75 % activity after a month of storage at 4 °C, which is six times more stable than the free enzyme. Immobilized enzyme was more stable at lower (3.5-4.5) and higher (6.5-7.0) pH ranges and higher temperatures (35-40 °C) compared with the free enzyme. Immobilized and free glucansucrase were employed in the acceptor reaction with maltose and each produced gluco-oligosaccharide ranging from trisaccharides to homologous pentasaccharides.
authors: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
authors: Damini
authors: D
authors: Kothari
authors: Rwivoo
authors: R
authors: Baruah
authors: Arun
authors: A
authors: Goyal
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D016695:Glycosyltransferases / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007934:Leuconostoc / Q000201:enzymology*; D009844:Oligosaccharides / Q000032:analysis / Q000378:metabolism*; D009994:Osmolar Concentration; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D009844:Oligosaccharides; D016695:Glycosyltransferases; C033837:alternansucrase