title: Identification of lactic acid bacteria isolated during traditional fura processing in Ghana.
abstract: Fura is a millet-based spontaneously fermented dumpling produced and consumed in parts of West Africa, particularly Nigeria, Burkina Faso and Ghana. From eight traditional fura production sites in northern Ghana, 862 lactic acid bacteria were isolated and identified to species level using a combination of genotypic and phenotypic methods including (GTG)(5)-based PCR fingerprinting and 16S rRNA gene sequencing, multiplex PCR by means of recA gene sequence comparison, conventional morphological characteristics and carbohydrate fermentation profiling. During millet dough fermentation, pH decreased from 5.6-6.4 to 4.1-3.7 and total lactic acid bacteria (LAB) counts increased from 4.4-5.3 to 7.9-9.2 log(10) (cfu/g). The initial stages of the fermentation were characterized by co-dominance of homo- and heterofermentative species of Pediococcus acidilactici, Weisella confusa, Lactobacillus fermentum, Lactobacillus reuteri, Lactobacillus salivarius, and Lactobacillus paraplantarum whereas L. fermentum was dominating at the end of the fermentation. L. fermentum was predominant in all fermentations (p < 0.05) and a high uniformity was observed among production sites regarding the dominance of L. fermentum. L. fermentum and W. confusa were isolated in all production sites and almost at all fermentation stages indicating that they are indigenous to traditional fura processing. The other LAB bacteria species which comprised a minor proportion of the total LAB occurred occasionally and in an irregular pattern among the production sites.
authors: Department of Applied Biology, University for Development Studies, Navrongo, Ghana.
authors: James
authors: J
authors: Owusu-Kwarteng
authors: Fortune
authors: F
authors: Akabanda
authors: Dennis S
authors: DS
authors: Nielsen
authors: Kwaku
authors: K
authors: Tano-Debrah
authors: Richard L K
authors: RL
authors: Glover
authors: Lene
authors: L
authors: Jespersen
mesh_terms: D005285:Fermentation; D005511:Food Handling; D005869:Ghana; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism*; D007777:Lactobacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D008897:Panicum / Q000737:chemistry / Q000382:microbiology*; D010802:Phylogeny
chemical_list: D019344:Lactic Acid