title: Carbonyl reductase mRNA abundance and enzymatic activity as potential biomarkers of oxidative stress in marine fish.
abstract: Carbonyl reductase (CBR) is an enzyme involved in protection from oxidative stress. In rainbow trout (Oncorhynchus mykiss), the hepatic mRNA abundance of the two isoforms (A and B) is increased after exposure to treated sewage effluents, as well as after exposure with β-naphthoflavone (β-NF) and the pro-oxidant paraquat. In this study, we show that the same chemicals similarly increase the single known hepatic CBR mRNA level and CBR catalytic activity in the coastal living eelpout (Zoarces viviparus). Hepatic CBR mRNA abundance and catalytic activity were also compared between eelpout collected at contaminated and reference sites on the Swedish west coast, but no differences were observed. In conclusion, CBR is a potential biomarker candidate for monitoring the exposure and effects of AhR agonists and/or pro-oxidants in the marine environment, but more research is needed to investigate temporal regulation as well as dose dependency for different chemicals. The mRNA and enzymatic assays presented in this study provide two additional tools for researchers interested in expanding their biomarker battery.
authors: Department of Biological and Environmental Sciences, University of Gothenburg, Box 463, SE-405 30 Göteborg, Sweden. eva.albertsson@bioenv.gu.se
authors: E
authors: E
authors: Albertsson
authors: A
authors: A
authors: Rad
authors: J
authors: J
authors: Sturve
authors: D G J
authors: DG
authors: Larsson
authors: L
authors: L
authors: Förlin
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D010269:Paraquat / Q000494:pharmacology; D010473:Perciformes / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity; D019324:beta-Naphthoflavone / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D019324:beta-Naphthoflavone; D000429:Alcohol Oxidoreductases; D010269:Paraquat