title: The adenoviral infections in children admitted to hospital with pneumonia, acute bronchiolitis or respiratory viral infections.
abstract: The objective of this study was to investigate the percent of infections with adenovirus (ADV) in children who had pneumonia, acute bronchiolitis or viral respiratory infections and were admitted to two pediatrics hospitals in Bucharest (Grigore Alexandrescu Hospital and Alfred Rusescu Hospital).
  
  
  METHODS
  
  
  
  METHODS
  70 children aged one month - five years, admitted to the above mentioned pediatrics hospitals in Bucharest, who were negative for the Respiratory Syncytial Virus (RSV) and the human Metapneumovirus (hMPV) by Reverse Transcription -Polymerase Chain Reaction (RT-PCR). 48 of them presented pneumonia upon admission to hospital, 6--acute bronchiolitis and 16 respiratory viral infections. Samples (nasal swabs) were taken from patients and introduced in viral transport medium.
  
  
  METHODS
  RT-PCR for RSV and hMPV, Multiplex PCR by seeplex multi-detection system with Seeplex RV/PB 18 ASE Detection for detection of 5 pneumonial bacteria and Real-Time PCR, Duplica Real Time Adenovirus Detection for ADV.
  
  
  RESULTS
  Of the total 70 patients negative for RSV, hMPV and 5 pneumonial bacteria (Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila), 10 were ADV positive; none of the children < 6 months (N = 16) presented ADV infection. In the 6 months - 2 years group (N = 35), 6 were ADV positive. In the 2 - 5 years group (N = 19), 4 were ADV positive.
  
  
  CONCLUSIONS
  The percent of ADV infections in children hospitalized with acute respiratory infections (ARI) caused by neither RSV or hMPV is 14.2%. ADV is most frequently encountered in the 6 months - 2 years and then 2 - 5 years groups, but the most severe pneumonia forms can be seen in the 6 months - 2 years group. In children < 6 months with acute bronchiolitis ADV was not found to be an etiologic agent.
authors: National Institute of Research - Development for Microbiology and Immunology Cantacuzino, Respiratory Viral Infections Department, Bucharest. tecucristina@yahoo.com
authors: Cristina
authors: C
authors: Tecu
authors: Maria E
authors: ME
authors: Mihai
authors: Viorel I
authors: VI
authors: Alexandrescu
authors: Dumitru
authors: D
authors: Orăşanu
authors: Carmen
authors: C
authors: Zapucioiu
authors: Dumitru
authors: D
authors: Matei
authors: M
authors: M
authors: Craiu
authors: Alexis
authors: A
authors: Cochino
mesh_terms: D000208:Acute Disease; D000260:Adenoviruses, Human / Q000302:isolation & purification*; D001988:Bronchiolitis / Q000821:virology*; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D011014:Pneumonia / Q000821:virology*; D012141:Respiratory Tract Infections / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods