title: Aggregation and conformational changes of bovine β-lactoglobulin subjected to dynamic high-pressure microfluidization in relation to antigenicity.
abstract: Our previous research indicated that dynamic high-pressure microfluidization (DHPM) had a significant effect on the antigenicity of β-lactoglobulin (β-LG). In this study, aggregation and conformational changes subjected to DHPM (0.1-160 MPa) were investigated in relation to antigenicity. When DHPM pressure increased from 0.1 to 80 MPa, disaggregation of β-LG samples and partial unfolding of the molecule were accompanied by an increase in β-LG antigenicity, which was reflected in the decrease of particle size, increase of free sulfhydryl (SH) contents and β-strands contents, and slight exposure of aromatic amino acid residues. At pressures above 80 MPa, the reaggregation of β-LG may contribute to the decrease in antigenicity, which was reflected by an increase in particle size, the formation of aggregates, a decrease of in SH and β-strands contents, and slight changes in aromatic amino acid residues. Aggregation and conformational changes of β-LG under DHPM was related to its antigenicity.
authors: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
authors: J Z
authors: JZ
authors: Zhong
authors: W
authors: W
authors: Liu
authors: C M
authors: CM
authors: Liu
authors: Q H
authors: QH
authors: Wang
authors: T
authors: T
authors: Li
authors: Z C
authors: ZC
authors: Tu
authors: S J
authors: SJ
authors: Luo
authors: X F
authors: XF
authors: Cai
authors: Y J
authors: YJ
authors: Xu
mesh_terms: D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D005260:Female; D007782:Lactoglobulins / Q000737:chemistry* / Q000276:immunology; D046210:Microfluidic Analytical Techniques / Q000379:methods; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D016269:Milk Hypersensitivity / Q000517:prevention & control*; D010316:Particle Size; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007782:Lactoglobulins