title: [Study on HPLC characteristic fingerprint of Yunnan Dipsacus asper].
abstract: OBJECTIVE
  To study the HPLC fingerprint of yunnan Dipsacus asper and provide a reliable method for sciencetific evaluation and quality control.
  
  
  METHODS
  16 batches of Dipsacus asper were collected from 11 counties (cities, districts) of 5 prefectures or municipals in Yunnan provinice, which were the mainly distribution region of Dipsacus asper. Samples were analyzed on an Aglient Zorbax SB-18 (4.6 mm x 250 mm, 5 microm) eluted with methanol (A) and water (B) as mobile phase in gradient clution. The flow rate was 1 ml/min, and the column temperature was set at 38 degrees C. The detector wavelength was 220 nm.
  
  
  RESULTS
  A HPLC fingerprint method was established, 14 common peaks were selected and the similarity ranged from 0.674 to 0.965, cluster analysis could classified these 16 batches of Dipsacus asper into 2 groups.
  
  
  CONCLUSIONS
  Dipsacus japonicus is found for the first time in yunnan. The fingerprint of Dipsacus asper in different origin have a extremely high similarity;The method is accurate and reliable.
authors: Institute of Chinese Medicinal Materials of Yunnan Agricultural University/Yunnan Provincial Center of Chinese Medicinal Materials' GAP Technology, Kunming 650201, China. yzg_10@163.com
authors: Zi-gang
authors: ZG
authors: Yang
authors: Gang
authors: G
authors: Xu
authors: Long-gen
authors: LG
authors: Li
authors: Zhen-gui
authors: ZG
authors: Meng
authors: Sheng-chao
authors: SC
authors: Yang
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016000:Cluster Analysis; D049917:Dipsacaceae / Q000737:chemistry* / Q000145:classification; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal