title: Ectopic overexpression of porcine DGAT1 increases intramuscular fat content in mouse skeletal muscle.
abstract: The microsomal enzyme 1, 2-acyl CoA: diacylglyceroltransferase-1 (DGAT1) plays an important role in triglyceride storage in adipose tissue and expresses in skeletal muscle as well. The primary goal of the present study was to investigate the effect of porcine DGAT1 on intramuscular fat (IMF) content of transgenic mice produced by pronuclear microinjection with muscle specific promoter of porcine muscle creatine kinase (MCK). In normal chow-fed diet, 4 month-old male transgenic mice expressed more DGAT1, ACC1, UCP1, and FABP4 mRNAs and proteins in skeletal muscle than control mice by real-time PCR and western blot. No significant changes were detected for ACC2, CD36, ADRP, PPAR                 gamma and LPL. Triacylglycerol assay and soleus muscle sections showed overexpression of porcine DGAT1 in skeletal muscle increased intramyocellular triglyceride and percent of the total cell surface covered by lipid droplets. Thus, upregulation of porcine DGAT1 in skeletal muscle increases IMF content. The present study may further serve to develop transgenic pigs with higher IMF content and improved meat quality.
authors: Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture and Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science, Huazhong Agricultural University, Wuhan, 430070, China.
authors: Ting
authors: T
authors: Li
authors: Dequan
authors: D
authors: Xu
authors: Bo
authors: B
authors: Zuo
authors: Minggang
authors: M
authors: Lei
authors: Yuanzhu
authors: Y
authors: Xiong
authors: Hongxing
authors: H
authors: Chen
authors: Yanhong
authors: Y
authors: Zhou
authors: Xiaojie
authors: X
authors: Wu
mesh_terms: D000818:Animals; D051048:Diacylglycerol O-Acyltransferase / Q000096:biosynthesis / Q000235:genetics; D015870:Gene Expression; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D013552:Swine / Q000235:genetics; D014280:Triglycerides / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D014280:Triglycerides; D051048:Diacylglycerol O-Acyltransferase