title: [Effect of lentiviral vector-mediated short hairpin RNA targeting survivin against endometriosis-like lesions in chick embryo chorioallantoic membrane].
abstract: OBJECTIVE
  To investigate the inhibitory effect of lentiviral vector-mediated short hairpin RNA targeting survivin (LV-survivin shRNA) on angiogenesis and growth of endometriosis-like lesions in chick en embryo chorioallantoic membrane.
  
  
  METHODS
  Eutopic endometrium from women with endometriosis was transplanted onto the non-vascular region of (CAM), where LV-survivin shRNA was delivered subsequently. The angiogenesis and the growth of endometriosis-like lesions in the CAM model were evaluated.
  
  
  RESULTS
  The angiogenesis and formation of endometriosis-like lesions were significantly suppressed in the CAM model by treatment with LV-survivin shRNA in comparison with those in the untreated CAM models and models treated with empty LV or DMEM (P<0.001). LV-survivin shRNA also caused a significantly higher cell apoptotic rate in the endometriosis-like lesions than the other treatments (P<0.001) and induced necrosis in the lesions.
  
  
  CONCLUSIONS
  LV-survivin shRNA can effectively inhibit angiogenesis induced by the eutopic endometrium and markedly suppress the formation of endometriosis-like lesions in the CAM model.
authors: Department of Gynecology and Obstetrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. pengdx1969@163.com
authors: Dongxian
authors: D
authors: Peng
authors: Yuanli
authors: Y
authors: He
authors: Liwen
authors: L
authors: Qiu
mesh_terms: D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000473:pathology*; D004195:Disease Models, Animal; D004715:Endometriosis / Q000235:genetics*; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics; D009389:Neovascularization, Pathologic / Q000473:pathology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D034741:RNA, Small Interfering; D000077022:Survivin