title: VopB1 and VopD1 are essential for translocation of type III secretion system 1 effectors of  Vibrio parahaemolyticus.
abstract: Vibrio parahaemolyticus  is a pathogenic  Vibrio  species that causes food-borne acute gastroenteritis, often related to the consumption of raw or undercooked seafood. Vibrio parahaemolyticus has 2 type III secretion systems (T3SS1 and T3SS2). Here, we demonstrate that VP1657 (VopB1) and VP1656 (VopD1), which share sequence similarity with Pseudomonas genes popB (38%) and popD (36%), respectively, are essential for translocation of T3SS1 effectors into host cells. A VP1680CyaA fusion reporter system was constructed to observe effector translocation. Using this reporter assay we showed that the VopB1 and VopD1 deletion strains were unable to translocate VP1680 to host cell but that the secretion of VP1680 into the culture medium was not affected. VopB1 or VopD1 deletion strains did not enhance cytotoxicity and failed to activate mitogen-activated protein kinases and secretion of interleukin-8, which depend on VP1680. Thus, we conclude that VopB1 and VopD1 are essential components of the translocon. To target VopB1 and VopD1 may have therapeutic potential for the treatment or prevention in V. parahaemolyticus infection.
authors: Department of Preventive Environment and Nutrition Institute of Health Biosciences, University of Tokushima Graduate School Kuromoto-cho 3-18-15, Tokushima City, Tokushima 770-8503, Japan. shimohata@nutr.med.tokushima-u.ac.jp
authors: Takaaki
authors: T
authors: Shimohata
authors: Kazuaki
authors: K
authors: Mawatari
authors: Hitomi
authors: H
authors: Iba
authors: Masakazu
authors: M
authors: Hamano
authors: Sachie
authors: S
authors: Negoro
authors: Shoko
authors: S
authors: Asada
authors: Mutsumi
authors: M
authors: Aihara
authors: Akiko
authors: A
authors: Hirata
authors: Zehong
authors: Z
authors: Su
authors: Akira
authors: A
authors: Takahashi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D058947:Bacterial Secretion Systems / Q000235:genetics*; D004789:Enzyme Activation / Q000235:genetics; D016209:Interleukin-8 / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport / Q000235:genetics; D017384:Sequence Deletion; D014736:Vibrio parahaemolyticus / Q000235:genetics* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D016209:Interleukin-8; D020928:Mitogen-Activated Protein Kinases