title: Marine cyclotripeptide X-13 promotes angiogenesis in zebrafish and human endothelial cells via PI3K/Akt/eNOS signaling pathways.
abstract: Cyclotripeptide X-13 is a core of novel marine compound xyloallenoide A isolated from mangrove fungus Xylaria sp. (no. 2508). We found that X-13 dose-dependently induced angiogenesis in zebrafish embryos and in human endothelial cells, which was accompanied by increased phosphorylation of eNOS and Akt and NO release. Inhibition of PI3K/Akt/eNOS by LY294002 or L-NAME suppressed X-13-induced angiogenesis. The present work demonstrates that X-13 promotes angiogenesis via PI3K/Akt/eNOS pathways.
authors: Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors: Xi-Lin
authors: XL
authors: Lu
authors: School of Chemistry & Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors: Zhong-Liang
authors: ZL
authors: Xu
authors: Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors: Xiao-Li
authors: XL
authors: Yao
authors: Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors: Feng-Juan
authors: FJ
authors: Su
authors: Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors: Cheng-Hui
authors: CH
authors: Ye
authors: School of Chemistry & Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors: Jing
authors: J
authors: Li
authors: School of Chemistry & Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors: Yong-Cheng
authors: YC
authors: Lin
authors: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.|Key Laboratory of Functional Molecules from Oceanic Microorganisms, Department of Education of Guangdong Province, Guangzhou 510080, China.
authors: Guang-Lei
authors: GL
authors: Wang
authors: Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors: Jin-Sheng
authors: JS
authors: Zeng
authors: Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors: Ru-Xun
authors: RX
authors: Huang
authors: Division of Cardiac Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.|The Key Laboratory of Assisted Circulation, Ministry of Health, Guangzhou 510182, China.
authors: Jing-Song
authors: JS
authors: Ou
authors: Division of Anatomy, Department of Surgery, Institute of Medical Science, Faculty of Medicine, University of Toronto, Toronto, ON M5G 1G6, Canada.
authors: Hong-Shuo
authors: HS
authors: Sun
authors: Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
authors: Li-Ping
authors: LP
authors: Wang
authors: School of Chemistry & Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors: Ji-Yan
authors: JY
authors: Pang
authors: Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors: Zhong
authors: Z
authors: Pei
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D059001:Aquatic Organisms / Q000737:chemistry*; D001688:Biological Products / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002867:Chromones / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D005658:Fungi / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009025:Morpholines / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D015027:Zebrafish / Q000378:metabolism
chemical_list: D043925:Angiogenesis Inducing Agents; D001688:Biological Products; D002867:Chromones; D009025:Morpholines; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one