title: LSI-based amperometric sensor for bio-imaging and multi-point biosensing.
abstract: We have developed an LSI-based amperometric sensor called "Bio-LSI" with 400 measurement points as a platform for electrochemical bio-imaging and multi-point biosensing. The system is comprised of a 10.4 mm × 10.4 mm CMOS sensor chip with 20 × 20 unit cells, an external circuit box, a control unit for data acquisition, and a DC power box. Each unit cell of the chip contains an operational amplifier with a switched-capacitor type I-V converter for in-pixel signal amplification. We successfully realized a wide dynamic range from ±1 pA to ±100 nA with a well-organized circuit design and operating software. In particular, in-pixel signal amplification and an original program to control the signal read-out contribute to the lower detection limit and wide detection range of Bio-LSI. The spacial resolution is 250 μm and the temporal resolution is 18-125 ms/400 points, which depends on the desired current detection range. The coefficient of variance of the current for 400 points is within 5%. We also demonstrated the real-time imaging of a biological molecule using Bio-LSI. The LSI coated with an Os-HRP film was successfully applied to the monitoring of the changes of hydrogen peroxide concentration in a flow. The Os-HRP-coated LSI was spotted with glucose oxidase and used for bioelectrochemical imaging of the glucose oxidase (GOx)-catalyzed oxidation of glucose. Bio-LSI is a promising platform for a wide range of analytical fields, including diagnostics, environmental measurements and basic biochemistry.
authors: Micro System Integration Center, Tohoku University, 519-1176 Aramaki-Aza-Aoba, Aoba-ku, Sendai, 980-0845, Japan. inoue@bioinfo.che.tohoku.ac.jp
authors: Kumi Y
authors: KY
authors: Inoue
authors: Masahki
authors: M
authors: Matsudaira
authors: Reyushi
authors: R
authors: Kubo
authors: Masanori
authors: M
authors: Nakano
authors: Shinya
authors: S
authors: Yoshida
authors: Sakae
authors: S
authors: Matsuzaki
authors: Atsushi
authors: A
authors: Suda
authors: Ryota
authors: R
authors: Kunikata
authors: Tatsuo
authors: T
authors: Kimura
authors: Ryota
authors: R
authors: Tsurumi
authors: Toshihito
authors: T
authors: Shioya
authors: Kosuke
authors: K
authors: Ino
authors: Hitoshi
authors: H
authors: Shiku
authors: Shiro
authors: S
authors: Satoh
authors: Masayoshi
authors: M
authors: Esashi
authors: Tomokazu
authors: T
authors: Matsue
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D004800:Enzymes, Immobilized; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose