title: Eukaryotic initiation factor 4E-3 is essential for meiotic chromosome segregation, cytokinesis and male fertility in Drosophila.
abstract: Gene expression is translationally regulated during many cellular and developmental processes. Translation can be modulated by affecting the recruitment of mRNAs to the ribosome, which involves recognition of the 5' cap structure by the cap-binding protein eIF4E. Drosophila has several genes encoding eIF4E-related proteins, but the biological role of most of them remains unknown. Here, we report that Drosophila eIF4E-3 is required specifically during spermatogenesis. Males lacking eIF4E-3 are sterile, showing defects in meiotic chromosome segregation, cytokinesis, nuclear shaping and individualization. We show that eIF4E-3 physically interacts with both eIF4G and eIF4G-2, the latter being a factor crucial for spermatocyte meiosis. In eIF4E-3 mutant testes, many proteins are present at different levels than in wild type, suggesting widespread effects on translation. Our results imply that eIF4E-3 forms specific eIF4F complexes that are essential for spermatogenesis.
authors: Department of Biology, McGill University, 3649 Promenade Sir William Osler, Montréal, Québec, H3G 0B1, Canada.
authors: Greco
authors: G
authors: Hernández
authors: Hong
authors: H
authors: Han
authors: Valentina
authors: V
authors: Gandin
authors: Lacramioara
authors: L
authors: Fabian
authors: Tiago
authors: T
authors: Ferreira
authors: Joanna
authors: J
authors: Zuberek
authors: Nahum
authors: N
authors: Sonenberg
authors: Julie A
authors: JA
authors: Brill
authors: Paul
authors: P
authors: Lasko
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002846:Chromatography, Affinity; D020090:Chromosome Segregation / Q000235:genetics / Q000502:physiology*; D048749:Cytokinesis / Q000235:genetics / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D004330:Drosophila / Q000378:metabolism / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism*; D005298:Fertility / Q000235:genetics / Q000502:physiology*; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008297:Male; D008540:Meiosis / Q000235:genetics / Q000502:physiology*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D013737:Testis / Q000166:cytology / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D017931:DNA Primers; D039561:Eukaryotic Initiation Factor-4E