title: Analysis of low temperature-induced genes (LTIG) in wine yeast during alcoholic fermentation.
abstract: Fermentations carried out at low temperatures, that is, 10-15 °C, not only enhance the production and retention of flavor volatiles, but also increase the chances of slowing or arresting the process. In this study, we determined the transcriptional activity of 10 genes that were previously reported as induced by low temperatures and involved in cold adaptation, during fermentation with the commercial wine yeast strain QA23. Mutant and overexpressing strains of these genes were constructed in a haploid derivative of this strain to determine the importance of these genes in growth and fermentation at low temperature. In general, the deletion and overexpression of these genes did affect fermentation performance at low temperature. Most of the mutants were unable to complete fermentation, while overexpression of CSF1, HSP104, and TIR2 decreased the lag phase, increased the fermentation rate, and reached higher populations than that of the control strain. Another set of overexpressing strains were constructed by integrating copies of these genes in the delta regions of the commercial wine strain QA23. These new stable overexpressing strains again showed improved fermentation performance at low temperature, especially during the lag and exponential phases. Our results demonstrate the convenience of carrying out functional analysis in commercial strains and in an experimental set-up close to industrial conditions.
authors: Biotecnologia Enològica, Departament de Bioquímica i Biotecnologia, Facultat de Enologia, Universitat Rovira i Virgili, Tarragona, Spain.
authors: Rosana
authors: R
authors: Chiva
authors: Maria
authors: M
authors: López-Malo
authors: Zoel
authors: Z
authors: Salvadó
authors: Albert
authors: A
authors: Mas
authors: Jósé Manuel
authors: JM
authors: Guillamón
mesh_terms: D003080:Cold Temperature; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D018628:Gene Dosage; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects*; D005800:Genes, Fungal; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics* / Q000528:radiation effects*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D014158:Transcription, Genetic; D014920:Wine / Q000382:microbiology
chemical_list: D000431:Ethanol