title: Application of a proteomic approach to identify proteins associated             with primary graft non-function after liver transplantation.
abstract: Primary graft non-function (PNF) is a rare, life-threatening complication             of liver transplantation. Increasing use of extended criteria donor pools and             high-risk recipients seem to influence the incidence of PNF. Primary failure is             associated with high patient morbidity and inferior graft survival. The only available             treatment for PNF is emergency hepatic retransplantation, which is also correlated             with significant morbidity and mortality. Therefore, researchers are working to             identify risk factors of diagnostic value to prevent PNF. The current study attempted             to explore liver proteomic patterns in patients with PNF. Using two-dimensional             gel electrophoresis and liquid chromatography-mass spectrometry (LC-MS), we compared             liver protein homogenates from 3 patients with PNF to those obtained from 6 healthy             liver samples to identify potential new biomarkers of PNF. Our comparisons revealed             21 proteins with differential expression (13 upregulated and 8 downregulated).             Most of these proteins are involved in energy metabolism, lipid metabolism, peptide             cleavage, cell differentiation, and apoptosis. Although none of these proteins             appeared more than once in separate analyses, this preliminary study shows that             two-dimensional gel electrophoresis and LC-MS may allow identification of characteristic             proteins to be used as biomarkers of a life-threatening complication of liver             transplantation. Larger-scale analyses could improve patient care by finding suitable             prognostic and therapeutic options. These data represent the first global proteomic             approach to study PNF.
authors: Department of General, Transplant and Liver Surgery, Medical University of Warsaw, 02-097 Warsaw, Poland.
authors: Oskar
authors: O
authors: Kornasiewicz
authors: Kamil
authors: K
authors: Bojarczuk
authors: Marek
authors: M
authors: Bugajski
authors: Jakub
authors: J
authors: Golab
authors: Marek
authors: M
authors: Krawczyk
mesh_terms: D000368:Aged; D002853:Chromatography, Liquid / Q000379:methods; D051799:Delayed Graft Function / Q000175:diagnosis* / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000503:physiopathology*; D016031:Liver Transplantation / Q000473:pathology*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D020543:Proteome / Q000032:analysis / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D020543:Proteome