title: The effect of octadecyl chain immobilization on the hemocompatibility of poly (2-hydroxyethyl methacrylate).
abstract: Albumin-scavenging surfaces decorated with n-alkyl chains represent an established strategy for blood-contacting applications. To evaluate this concept, a set of poly (2-hydroxyethyl methacrylate) (pHEMA) films modified with different amounts of octadecyl isocyanate (C18) was investigated in an in vitro hemocompatibility assay using freshly drawn human whole blood. In addition, the hydrogel materials were thoroughly characterized with respect to changes in wettability and elasticity, which accompanied the gradual chemical modification of pHEMA. An increase of the surface C18 content induced enhanced hydrophobicity and stiffness. Immobilization of C18 chains was found to substantially reduce the coagulation activation and the complement activation by the pHEMA films. Platelet adhesion and degranulation (PF4 release) were similar on the modified and the unmodified pHEMA. Platelet adhesion to pHEMA hydrogels was lower than the polytetrafluoroethylene reference. We conclude that the immobilization of octadecyl chains improved the hemocompatibility of pHEMA materials under conditions that might be encountered in low shear blood flow.
authors: Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Dresden, Germany.
authors: Marion
authors: M
authors: Fischer
authors: Catarina P
authors: CP
authors: Baptista
authors: Inês C
authors: IC
authors: Gonçalves
authors: Buddy D
authors: BD
authors: Ratner
authors: Claudia
authors: C
authors: Sperling
authors: Carsten
authors: C
authors: Werner
authors: Cristina L
authors: CL
authors: Martins
authors: Mário A
authors: MA
authors: Barbosa
mesh_terms: D000991:Antithrombins / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D039481:CD11b Antigen / Q000378:metabolism; D015936:Complement C5a / Q000378:metabolism; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D017953:Isocyanates / Q000737:chemistry; D055595:Mechanical Phenomena / Q000187:drug effects; D008856:Microscopy, Fluorescence; D010973:Platelet Adhesiveness / Q000187:drug effects; D010976:Platelet Count; D010978:Platelet Factor 4 / Q000378:metabolism; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry* / Q000494:pharmacology*; D012995:Solubility; D013917:Thrombin / Q000378:metabolism
chemical_list: D000991:Antithrombins; D001672:Biocompatible Materials; D039481:CD11b Antigen; D020100:Hydrogels; D017953:Isocyanates; D011102:Polyhydroxyethyl Methacrylate; D010978:Platelet Factor 4; D015936:Complement C5a; D013917:Thrombin