title: Loss of HITS (FAM107B) expression in cancers of multiple organs: tissue microarray analysis.
abstract: Family with sequence similarity 107 (FAM107) proteins consist of two subtypes, FAM107A and FAM107B in mammals, possessing a conserved N-terminal domain of unknown function. Recently we found that FAM107B, an 18 kDa nuclear protein, is expressed in a broad range of tissues and is downregulated in gastrointestinal cancer. Because FAM107B expression is amplified by heat-shock stimulation, we designated it heat shock-inducible tumor small protein (HITS). Although data related to FAM107A as a candidate tumor suppressor have been accumulated, little biological information is available for HITS. In the present study, we examined HITS expression using immunohistochemistry with tissue microarrays and performed detailed statistical analyses. By screening a high-density multiple organ tumor and normal tissue microarray, HITS expression was decreased in tumor tissues of the breast, thyroid, testis and uterine cervix as well as the stomach and colon. Further analysis of tissue microarrays of individual organs showed that loss of HITS expression in cancer tissues was statistically significant and commonly observed in distinct organs in a histological type-specific manner. The HITS expression intensity was inversely correlated with the primary tumor size in breast and thyroid cancers. In addition, effects of tetracycline-inducible HITS expression on tumor growth were investigated in vivo. Forced expression of HITS inhibited tumor xenograft proliferation, compared with the mock-treated tumor xenograft model. These results show that loss of HITS expression is a common phenomenon observed in cancers of distinct organs and involved in tumor development and proliferation.
authors: Department of Oncology, Ageo Central General Hospital, Ageo, Saitama, Japan. nakajima.h@ach.or.jp
authors: Hideo
authors: H
authors: Nakajima
authors: Keita
authors: K
authors: Koizumi
authors: Takuji
authors: T
authors: Tanaka
authors: Yasuhito
authors: Y
authors: Ishigaki
authors: Yoshino
authors: Y
authors: Yoshitake
authors: Hideto
authors: H
authors: Yonekura
authors: Tsutomu
authors: T
authors: Sakuma
authors: Toshihiro
authors: T
authors: Fukushima
authors: Hisanori
authors: H
authors: Umehara
authors: Soichiro
authors: S
authors: Ueno
authors: Toshinari
authors: T
authors: Minamoto
authors: Yoshiharu
authors: Y
authors: Motoo
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics*; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006867:Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics* / Q000473:pathology; D046888:Tissue Array Analysis; D023041:Xenograft Model Antitumor Assays
chemical_list: C574381:ABHD17B protein, human; D006867:Hydrolases