title: Characterization of carbonic anhydrase II from Chlorella vulgaris in bio-CO2 capture.
abstract: Carbonic anhydrase II (CA II) can catalyze the reversible hydration reaction of CO(2) at a maximum of 1.4 × 10(6) molecules of CO(2) per second. The crude intracellular enzyme extract containing CA II was derived from Chlorella vulgaris. A successful CO(2) capture experiment with the presence of calcium had been conducted on the premise that the temperature was conditioned at a scope of 30-40 °C, that the biocatalyst-nurtured algal growth period lasted 3 days, and that pH ranged from7.5 to 8.5. Ions of K(+), Na(+), Ca(2+), Co(2+), Cu(2+), Fe(3+), Mg(2+), Mn(2+), and Zn(2+) at 0.01, 0.1, and 0.5 M were found to exhibit no more than 30 % inhibition on the residual activity of the biocatalyst. It is reasonable to expect that calcification catalyzed by microalgae presents an alternative to geological carbon capture and sequestration through a chain of fundamental researches carried on under the guidance of sequestration technology.
authors: Nano-structural Materials Center, School of Materials Science and Engineering, Nanjing University of Science and Technology, Street of Xiao Ling Wei 200, Nanjing, 210094, China. lili_luoyang@126.com
authors: Li
authors: L
authors: Li
authors: Ming-Lai
authors: ML
authors: Fu
authors: Yong-Hao
authors: YH
authors: Zhao
authors: Yun-Tian
authors: YT
authors: Zhu
mesh_terms: D002245:Carbon Dioxide / Q000032:analysis / Q000378:metabolism*; D057965:Carbon Sequestration; D024402:Carbonic Anhydrase II / Q000032:analysis / Q000378:metabolism*; D048408:Chlorella vulgaris / Q000254:growth & development / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
chemical_list: D002245:Carbon Dioxide; D024402:Carbonic Anhydrase II