title: Expression of NADPH oxidase in human pancreatic islets.
abstract: OBJECTIVE
  NADPH oxidase (NOX) is a known source of superoxide anions in phagocytic and non-phagocytic cells. In this study, the presence of this enzyme in human pancreatic islets and the importance of NADPH oxidase in human β-cell function were investigated.
  
  
  RESULTS
  In isolated human pancreatic islets, the expression of NADPH oxidase components was evidenced by real-time PCR (p22(PHOX), p47(PHOX) and p67(PHOX)), Western blotting (p47(PHOX) and p67(PHOX)) and immunohistochemistry (p47(PHOX), p67(PHOX) and gp91(PHOX)). Immunohistochemistry experiments showed co-localization of p47(PHOX), p67(PHOX) and gp91(PHOX) (isoform 2 of NADPH oxidase-NOX2) with insulin secreting cells. Inhibition of NADPH oxidase activity impaired glucose metabolism and glucose-stimulated insulin secretion.
  
  
  CONCLUSIONS
  These findings demonstrate the presence of the main intrinsic components of NADPH oxidase comprising the NOX2 isoform in human pancreatic islets, whose activity also contributes to human β-cell function.
authors: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil. rebelato@icb.usp.br
authors: Eduardo
authors: E
authors: Rebelato
authors: Thiago R
authors: TR
authors: Mares-Guia
authors: Maria Fernanda R
authors: MF
authors: Graciano
authors: Letícia
authors: L
authors: Labriola
authors: Luiz R G
authors: LR
authors: Britto
authors: Humberto M
authors: HM
authors: Garay-Malpartida
authors: Rui
authors: R
authors: Curi
authors: Mari C
authors: MC
authors: Sogayar
authors: Angelo R
authors: AR
authors: Carpinelli
mesh_terms: D000328:Adult; D001483:Base Sequence; D015153:Blotting, Western; D017931:DNA Primers; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000201:enzymology* / Q000378:metabolism; D008875:Middle Aged; D019255:NADPH Oxidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004791:Enzyme Inhibitors; D007328:Insulin; D019255:NADPH Oxidases; D005947:Glucose