title: Anti-hepatitis B virus constituents from the stem bark of Streblus asper.
abstract: Seven compounds, (7'S,8'S)-trans-streblusol A, (7'R,8'S)-erythro-streblusol B, (7'S,8'S)-threo-streblusol B, 8'R-streblusol C, streblusquinone, (8R,8'R)-streblusol D, and streblusol E, along with 15 known compounds (8-22) were isolated from the n-butanol-soluble part of the MeOH extract of stem bark of Streblus asper. Their structures were elucidated through application of extensive spectroscopic methods, including ESI-MS and 2D NMR spectroscopy (HMQC and HMBC). The stereochemistry at the chiral centers was determined using CD spectra, as well as analyses of coupling constants and optical rotation data. The isolated lignans and allylbenzene derivatives were evaluated for their anti-HBV activities in vitro using the HBV transfected Hep G2.2.15 cell line. The most active compounds, magnolol and 9-β-xylopyranosyl-isolariciresinol, exhibited significant anti-HBV activities with IC(50) values of 2.03 and 6.58μM for secretion of HBsAg, with no cytotoxicity, and of 3.76 and 24.86μM for secretion of HBeAg, with no cytotoxicity.
authors: Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Ministry of Education of China), School of Environment and Resource of Guangxi Normal University, Guilin, PR China. lijun9593@mailbox.gxnu.edu.cn
authors: Jun
authors: J
authors: Li
authors: Yan
authors: Y
authors: Huang
authors: Xin-Lan
authors: XL
authors: Guan
authors: Jian
authors: J
authors: Li
authors: Sheng-Ping
authors: SP
authors: Deng
authors: Qiang
authors: Q
authors: Wu
authors: Yan-Jun
authors: YJ
authors: Zhang
authors: Xiao-Jian
authors: XJ
authors: Su
authors: Rui-Yun
authors: RY
authors: Yang
mesh_terms: D000998:Antiviral Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D000432:Methanol / Q000737:chemistry; D029586:Moraceae / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D010936:Plant Extracts; D000432:Methanol