title: Elevated interleukin-32 expression in granulomatosis with polyangiitis.
abstract: OBJECTIVE
  To evaluate the role of IL-32 in granulomatosis with polyangiitis (GPA) patients and the relationship between IL-32 and disease activity, as PR3 has the ability to bind and activate IL-32, which has been described as a novel cytokine that induces inflammatory cytokines.
  
  
  METHODS
  We investigated the level of IL-32, PR3, TNF-α and IL-6 in GPA patients by using ELISA. Northern blot was used to analyse the level of IL-32 mRNA in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes was examined by IF staining.
  
  
  RESULTS
  We observed that IL-32 and PR3 levels in GPA patients were increased significantly when compared with normal individuals and each was tightly associated (P < 0.001). Northern blot analysis revealed that the mRNA level of IL-32 was prominently elevated in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes from GPA patients vs normal individuals was verified by IF staining.
  
  
  CONCLUSIONS
  IL-32 level was elevated in GPA patients but its level was changed by treatment response. IL-32 could be an index in GPA and play a role in the aetiology of GPA.
authors: Laboratory of Cytokine Immunology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 143-701 Korea.
authors: Suyoung
authors: S
authors: Bae
authors: Yong-Gil
authors: YG
authors: Kim
authors: Jida
authors: J
authors: Choi
authors: Jaewoo
authors: J
authors: Hong
authors: Siyoung
authors: S
authors: Lee
authors: Taebong
authors: T
authors: Kang
authors: Hyunjhung
authors: H
authors: Jeon
authors: Kwangwon
authors: K
authors: Hong
authors: Eusom
authors: E
authors: Kim
authors: Areum
authors: A
authors: Kwak
authors: Chang-Keun
authors: CK
authors: Lee
authors: Bin
authors: B
authors: Yoo
authors: Yong-Beom
authors: YB
authors: Park
authors: Eun Young
authors: EY
authors: Song
authors: Soohyun
authors: S
authors: Kim
mesh_terms: D000328:Adult; D000368:Aged; D015152:Blotting, Northern; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D014890:Granulomatosis with Polyangiitis / Q000209:etiology* / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism / Q000502:physiology*; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D053146:Myeloblastin / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014657:Vasculitis / Q000209:etiology / Q000378:metabolism
chemical_list: C496971:IL32 protein, human; D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D053146:Myeloblastin