title: Small molecules that regulate zymosan phagocytosis of macrophage through deactivation of Rho GTPases.
abstract: Phagocytosis and subsequent degradation of pathogens by macrophages play a pivotal role in host innate immune response to microbial infections. To find small molecule regulators for the investigation of complicated phagocytic process, we screened our in-house chemical library and found chemicals which inhibit phagocytosis of zymosan by macrophages. A representative compound 5a reduced phagocytosis of zymosan in both peritoneal macrophages and RAW264.7 cells in a dose-dependent manner. Treatment of 5a led to downregulate the key regulators of phagocytosis, Rac1, Rac2 and Cdc42, and slightly reduced phosphorylation of Akt by zymosan.
authors: Laboratory of Host Defense Modulation, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors: Joon Seok
authors: JS
authors: Bang
authors: Young Jin
authors: YJ
authors: Kim
authors: Jiho
authors: J
authors: Song
authors: Jong-Sun
authors: JS
authors: Yoo
authors: Seul
authors: S
authors: Lee
authors: Mi Ji
authors: MJ
authors: Lee
authors: Hyeyoung
authors: H
authors: Min
authors: Kwang Woo
authors: KW
authors: Hwang
authors: Kyung Hoon
authors: KH
authors: Min
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D010587:Phagocytosis / Q000187:drug effects*; D054852:Small Molecule Libraries / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D015054:Zymosan / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D054852:Small Molecule Libraries; D015054:Zymosan; D020741:rho GTP-Binding Proteins