title: Wettability influences cell behavior on superhydrophobic surfaces with different topographies.
abstract: Surface wettability and topography are recognized as critical factors influencing cell behavior on biomaterials. So far only few works have reported cell responses on surfaces exhibiting extreme wettability in combination with surface topography. The goal of this work is to study whether cell behavior on superhydrophobic surfaces is influenced by surface topography and polymer type. Biomimetic superhydrophobic rough surfaces of polystyrene and poly(L-lactic acid) with different micro/nanotopographies were obtained from smooth surfaces using a simple phase-separation based method. Total protein was quantified and showed a less adsorption of bovine serum albumin onto rough surfaces as compared to smooth surfaces of the same material. The mouse osteoblastic MC3T3-E1 cell line and primary bovine articular chondrocytes were used to study cell attachment and proliferation. Cells attached and proliferate better in the smooth surfaces. The superhydrophobic surfaces allowed cells to adhere but inhibited their proliferation. This study indicates that surface wettability, rather than polymer type or the topography of the superhydrophobic surfaces, is a critical factor in determining cell behavior.
authors: 3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, University of Minho, AvePark, Taipas, Guimarães, Portugal.
authors: Bianca N
authors: BN
authors: Lourenço
authors: Giulia
authors: G
authors: Marchioli
authors: Welong
authors: W
authors: Song
authors: Rui L
authors: RL
authors: Reis
authors: Clemens A
authors: CA
authors: van Blitterswijk
authors: Marcel
authors: M
authors: Karperien
authors: Aart
authors: A
authors: van Apeldoorn
authors: João F
authors: JF
authors: Mano
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002417:Cattle; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D019902:Chondrocytes / Q000502:physiology*; D003124:Colorimetry; D005470:Fluorometry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology*; D013499:Surface Properties; D017155:Wettability
chemical_list: D001672:Biocompatible Materials