title: Methylenetetrahydrofolate reductase C677T gene polymorphism and colorectal cancer risk: A case-control study.
abstract: We designed a case-control study to determine the plasma homocysteine (Hcy) level and evaluate the potential role of the methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism in colorectal cancer (CRC). Total Hcy was quantified using the fluorescence polarization immunoassay (FPIA) on the IMx analyzer. Genomic DNA was analyzed by the real-time polymerase chain reaction (RT-PCR). The plasma levels of Hcy in the CRC group (12.63±3.11 μmol/l) were significantly higher compared with those in the control group (10.87±2.42 μmol/l; P<0.05). The frequency of the MTHFR 677TT genotype in CRC patients was markedly high. The MTHFR 677TT genotype was significantly correlated with an increased risk of CRC (odds ratio, 1.671; 95% confidence interval, 1.094-2.553; P=0.018). This study suggests that the MTHFR C677T polymorphism indicates susceptibility to CRC and is correlated with CRC pathogenesis, suggesting that the homozygous variant MTHFR C677T polymorphism is a candidate risk factor for CRC.
authors: Department of Vascular Surgery, Shandong Provincial Hospital, Shandong University, Jinan 250012.
authors: Guancheng
authors: G
authors: Yin
authors: Hanxin
authors: H
authors: Ming
authors: Xiao
authors: X
authors: Zheng
authors: Yi
authors: Y
authors: Xuan
authors: Jianwei
authors: J
authors: Liang
authors: Xing
authors: X
authors: Jin