title: Murine monoclonal antibody to platelet factor 4/heparin complexes as a potential reference standard for platelet activation assays in heparin-induced thrombocytopenia.
abstract: Quality control of the platelet activation assays to diagnose heparin-induced thrombocytopenia (HIT), (14)C-serotonin release assay (SRA) and platelet aggregation test (PAT) has yet to be established due to lack of reference standards and the difficulty of obtaining significant amounts of HIT antibodies from patients with HIT. We prepared a murine monoclonal antibody to human platelet factor 4 (hPF4)/heparin complexes (HIT-MoAb) and investigated the platelet activating action of HIT-MoAb by using SRA and PAT. The HIT-MoAb activated human platelets at low heparin concentration and the platelet activations were inhibited at high heparin concentration in both SRA and PAT. The HIT-MoAb produced a concentration-dependent effect. Moreover, the platelet activation at low heparin concentration was inhibited by anti-FcγRIIa antibody. These results indicated that HIT-MoAb has characteristics similar to human HIT antibodies regarding heparin-dependent platelet activation. Therefore, it is suggested that HIT-MoAb has the potential to be a positive control or reference standard in platelet activation assays.
authors: Department of Clinical Laboratory, Hyogo Cancer Center, Hyogo, Japan. katopoppo-reiko@gaia.eonet.ne.jp
authors: Reiko
authors: R
authors: Asada
authors: Keiko
authors: K
authors: Wanaka
authors: Jeanine
authors: J
authors: Walenga
authors: Margaret
authors: M
authors: Prechel
authors: Kumiko
authors: K
authors: Miyashita
authors: Vicki
authors: V
authors: Escalante
authors: Chieko
authors: C
authors: Kaneko
authors: Nobuhiro
authors: N
authors: Hoshino
authors: Mitsuru
authors: M
authors: Oosawa
authors: Miyako
authors: M
authors: Matsuo
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry* / Q000276:immunology; D000925:Anticoagulants / Q000009:adverse effects* / Q000097:blood* / Q000627:therapeutic use; D005260:Female; D006493:Heparin / Q000009:adverse effects* / Q000097:blood* / Q000627:therapeutic use; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D010974:Platelet Aggregation / Q000187:drug effects; D010978:Platelet Factor 4 / Q000097:blood* / Q000276:immunology; D010979:Platelet Function Tests / Q000379:methods / Q000592:standards; D012015:Reference Standards; D013921:Thrombocytopenia / Q000097:blood* / Q000139:chemically induced* / Q000276:immunology
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000925:Anticoagulants; D010978:Platelet Factor 4; D006493:Heparin