title: Lipoxin A₄ inhibits platelet-activating factor inflammatory response and stimulates corneal wound healing of injuries that compromise the stroma.
abstract: Platelet-activating factor (PAF) is a bioactive lipid mediator with strong inflammatory properties. PAF induces the expression and activation of metalloproteinase-9 (MMP-9) in corneal epithelial cells and myofibroblasts, and delays epithelial wound healing in an organ culture system. Lipoxin A(4) (LXA(4)) is a lipid mediator involved in resolution of inflammation and cornea epithelial wound healing. We developed an in vivo mouse model of injury to the anterior stroma that is sustained by PAF and evaluated the action of LXA(4). In this model mice were treated with vehicle, PAF alone and in combination with PAF receptor antagonist LAU-0901 or LXA(4). Mice were euthanized 1, 2 and 7 days after injury and corneas were processed for histology (H&E staining) and immunofluorescence with antibodies for MMP-9, α-smooth muscle actin (α-SMA), fibronectin (FN) and neutrophil. Interleukin 1-α (IL-1α) and keratinocyte-derived chemokine (KC/CXCL1) were assayed by ELISA. Myeloperoxidase (MPO) activity was performed in corneal homogenates. In this in vivo model PAF inhibited epithelial wound healing that was blocked by the PAF receptor antagonist LAU-0901. Treatment with LXA(4) significantly reduced the injured area compared to PAF at 1 and 2 days of treatment. The strong stromal cell infiltration and MPO activity stimulated by PAF was also decreased with LXA(4) treatment. PAF increased MMP-9 and decreased FN expression compared to vehicle treatment and less α-SMA positive cells migrated to the wounded area. The PAF actions were reverted by LXA(4) treatment. The results demonstrated a powerful action of LXA(4) in protecting corneas with injuries that compromise the stroma by decreasing inflammation and increasing wound healing.
authors: Louisiana State University Health Sciences Center, Department of Ophthalmology and The Neuroscience Center of Excellence, 2020 Gravier Street, New Orleans, LA 70112, USA.
authors: Azucena
authors: A
authors: Kakazu
authors: Jiucheng
authors: J
authors: He
authors: Sachidananda
authors: S
authors: Kenchegowda
authors: Haydee E P
authors: HE
authors: Bazan
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D054360:Chemokine CXCL1 / Q000378:metabolism; D003319:Corneal Stroma / Q000293:injuries*; D004095:Dihydropyridines / Q000494:pharmacology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005131:Eye Injuries / Q000188:drug therapy*; D005353:Fibronectins / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D053582:Interleukin-1alpha / Q000378:metabolism; D044045:Lipoxins / Q000494:pharmacology*; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000502:physiology; D009195:Peroxidase / Q000378:metabolism; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D010980:Platelet Membrane Glycoproteins / Q000037:antagonists & inhibitors; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors; D014945:Wound Healing / Q000187:drug effects*
chemical_list: C558396:Acta2 protein, mouse; D000199:Actins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D054360:Chemokine CXCL1; C475895:Cxcl1 protein, mouse; D004095:Dihydropyridines; D005353:Fibronectins; D053582:Interleukin-1alpha; C487837:LAU 0901; D044045:Lipoxins; D010972:Platelet Activating Factor; D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; C040527:lipoxin A4; C039351:platelet activating factor receptor; D009195:Peroxidase; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse