title: Association of polymorphisms of cytokine and TLR-2 genes with long-term immunity to hepatitis B in children vaccinated early in life.
abstract: Hepatitis B vaccine is effective in preventing hepatitis B virus (HBV) infection. However, 5-10% of vaccinees fail to produce sufficient antibody against hepatitis B surface antigen (anti-HBs). In this study, we investigated the association of genetic polymorphisms with long-term response to hepatitis B vaccine in 301 children who received the vaccine 5-7 years ago. Of them, 86 (28.6%) had anti-HBs <10 mIU/ml (group A) and 215 (71.4%) had anti-HBs ≥10 mIU/ml (group B). While the frequencies of T allele and TT genotype in single nucleotide polymorphisms (SNP) rs2243250 and rs2070874 of interleukin (IL)-4 in group A were higher than those in group B (all P<0.05 and q<0.2), the frequency of C allele in SNP rs2243250, rs2070874 and rs2227284 of IL-4 in group B was higher than that in group A (all P<0.05 and q<0.2). None of 11 other SNP in IL-2, IL-10, IL-1β, IL-13, IL-12B, tumor necrosis factor-α, and toll-like receptor-2 genes was found to associate with anti-HBs response. SNP rs2070874 was associated with humoral response to hepatitis B vaccine after analyzed by multivariable logistic regression analysis (P=0.015). The haplotype TT defined by SNP rs2243250 and rs2070874 in IL-4 was associated with the poor humoral response (adjusted P=0.037). Our findings demonstrate that IL-4 gene polymorphisms may affect the long-term immune response to hepatitis B vaccine.
authors: Department of Laboratory Medicine, Nanjing Drum Tower Hospital, Nanjing University Medical School, Jiangsu, China.
authors: Yangyang
authors: Y
authors: Wang
authors: Peizhen
authors: P
authors: Xu
authors: Dongmei
authors: D
authors: Zhu
authors: Shu
authors: S
authors: Zhang
authors: Yongchun
authors: Y
authors: Bi
authors: Yali
authors: Y
authors: Hu
authors: Yi-Hua
authors: YH
authors: Zhou
mesh_terms: D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000235:genetics; D005260:Female; D006239:Haplotypes; D006509:Hepatitis B / Q000276:immunology* / Q000517:prevention & control; D006510:Hepatitis B Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D017325:Hepatitis B Vaccines; D006801:Humans; D056724:Immunity, Humoral; D007156:Immunologic Memory; D015847:Interleukin-4 / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D051195:Toll-Like Receptor 2 / Q000235:genetics*
chemical_list: D016207:Cytokines; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; C508598:IL4 protein, human; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2; D015847:Interleukin-4