title: Hsp90 binds microtubules and is involved in the reorganization of the microtubular network in angiosperms.
abstract: Microtubules (MTs) are essential for many processes in plant cells. MT-associated proteins (MAPs) influence MT polymerization dynamics and enable them to perform their functions. The molecular chaperone Hsp90 has been shown to associate with MTs in animal and plant cells. However, the role of Hsp90-MT binding in plants has not yet been investigated. Here, we show that Hsp90 associates with cortical MTs in tobacco cells and decorates MTs in the phragmoplast. Further, we show that tobacco Hsp90_MT binds directly to polymerized MTs in vitro. The inhibition of Hsp90 by geldanamycin (GDA) severely impairs MT re-assembly after cold-induced de-polymerization. Our results indicate that the plant Hsp90 interaction with MTs plays a key role in cellular events, where MT re-organization is needed.
authors: Department of Experimental Plant Biology, Charles University in Prague, Viničná 5, 128 44 Prague 2, Czech Republic. akcni.krtecek@post.cz
authors: Jana
authors: J
authors: Krtková
authors: Aleksandra
authors: A
authors: Zimmermann
authors: Kateřina
authors: K
authors: Schwarzerová
authors: Peter
authors: P
authors: Nick
mesh_terms: D000595:Amino Acid Sequence; D016227:Benzoquinones / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D047029:Lactams, Macrocyclic / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism*; D058105:Polymerization / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism
chemical_list: D016227:Benzoquinones; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D010940:Plant Proteins; D011994:Recombinant Proteins; D014404:Tubulin; D049452:Green Fluorescent Proteins; C001277:geldanamycin