title: Increased hypothalamic inflammation associated with the susceptibility to obesity in rats exposed to high-fat diet.
abstract: Inflammation has been implicated in the hypothalamic leptin and insulin resistance resulting defective food intake during high fat diet period. To investigate hypothalamic inflammation in dietary induced obesity (DIO) and obesity resistant (DIO-R) rats, we established rat models of DIO and DIO-R by feeding high fat diet for 10 weeks. Then we switched half of DIO and DIO-R rats to chow food and the other half to high fat diet for the following 8 weeks to explore hypothalamic inflammation response to the low fat diet intervention. Body weight, caloric intake, HOMA-IR, as well as the mRNA expression of hypothalamic TLR4, NF-κB, TNF-α, IL-1β, and IL-6 in DIO/HF rats were significantly increased compared to DIO-R/HF and CF rats, whereas IL-10 mRNA expression was lower in both DIO/HF and DIO-R/HF rats compared with CF rats. Switching to chow food from high fat diet reduced the body weight and improved insulin sensitivity but not affecting the expressions of studied inflammatory genes in DIO rats. Take together, upregulated hypothalamic inflammation may contribute to the overeating and development of obesity susceptibility induced by high fat diet. Switching to chow food had limited role in correcting hypothalamic inflammation in DIO rats during the intervention period.
authors: Department of Occupational and Environment Health, School of Public Health, Nantong University, Nantong 226019, China.
authors: Xiaoke
authors: X
authors: Wang
authors: Aiguo
authors: A
authors: Ge
authors: Mengjie
authors: M
authors: Cheng
authors: Fangfang
authors: F
authors: Guo
authors: Min
authors: M
authors: Zhao
authors: Xiaoqi
authors: X
authors: Zhou
authors: Liegang
authors: L
authors: Liu
authors: Nianhong
authors: N
authors: Yang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D020022:Genetic Predisposition to Disease; D007031:Hypothalamus / Q000378:metabolism*; D007249:Inflammation / Q000473:pathology*; D007328:Insulin / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D001786:Blood Glucose; D007328:Insulin; D012333:RNA, Messenger