title: Graphene-based aptamer logic gates and their application to multiplex detection.
abstract: In this work, a GO/aptamer system was constructed to create multiplex logic operations and enable sensing of multiplex targets. 6-Carboxyfluorescein (FAM)-labeled adenosine triphosphate binding aptamer (ABA) and FAM-labeled thrombin binding aptamer (TBA) were first adsorbed onto graphene oxide (GO) to form a GO/aptamer complex, leading to the quenching of the fluorescence of FAM. We demonstrated that the unique GO/aptamer interaction and the specific aptamer-target recognition in the target/GO/aptamer system were programmable and could be utilized to regulate the fluorescence of FAM via OR and INHIBIT logic gates. The fluorescence changed according to different input combinations, and the integration of OR and INHIBIT logic gates provided an interesting approach for logic sensing applications where multiple target molecules were present. High-throughput fluorescence imagings that enabled the simultaneous processing of many samples by using the combinatorial logic gates were realized. The developed logic gates may find applications in further development of DNA circuits and advanced sensors for the identification of multiple targets in complex chemical environments.
authors: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
authors: Li
authors: L
authors: Wang
authors: Jinbo
authors: J
authors: Zhu
authors: Lei
authors: L
authors: Han
authors: Lihua
authors: L
authors: Jin
authors: Chengzhou
authors: C
authors: Zhu
authors: Erkang
authors: E
authors: Wang
authors: Shaojun
authors: S
authors: Dong
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D039301:Computers, Molecular; D004581:Electronics / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D010316:Particle Size; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*
chemical_list: D052157:Aptamers, Nucleotide; D006108:Graphite