title: Human S100A3 tetramerization propagates Ca(2+)/Zn(2+) binding states.
abstract: The S100A3 homotetramer assembles upon citrullination of a specific symmetric Arg51 pair on its homodimer interface in human hair cuticular cells. Each S100A3 subunit contains two EF-hand-type Ca(2+)-binding motifs and one (Cys)3His-type Zn(2+)-binding site in the C-terminus. The C-terminal coiled domain is cross-linked to the presumed docking surface of the dimeric S100A3 via a disulfide bridge. The aim of this study was to determine the structural and functional role of the C-terminal Zn(2+)-binding domain, which is unique to S100A3, in homotetramer assembly. The binding of either Ca(2+) or Zn(2+) reduced the α-helix content of S100A3 and modulated its affinity for the other cation. The binding of a single Zn(2+) accelerated the Ca(2+)-dependent tetramerization of S100A3 while inducing an extensive unfolding of helix IV. The Ca(2+) and Zn(2+) binding affinities of S100A3 were enhanced when the other cation bound in concert with the tetramerization of S100A3. Small angle scattering analyses revealed that the overall structure of the S100A3 tetramer bound both Ca(2+) and Zn(2+) had a similar molecular shape to the Ca(2+)-bound form in solution. The binding states of the Ca(2+) or Zn(2+) to each S100A3 subunit within a homotetramer appear to be propagated by sensing the repositioning of helix III and the rearrangement of the C-terminal tail domain. This article is part of a Special Issue entitled: 12th European Symposium on Calcium.
authors: Innovative Beauty Science Laboratory, Kanebo Cosmetics Inc., Odawara, Japan. kizawa.kenji@kanebocos.co.jp
authors: Kenji
authors: K
authors: Kizawa
authors: Yuji
authors: Y
authors: Jinbo
authors: Takafumi
authors: T
authors: Inoue
authors: Hidenari
authors: H
authors: Takahara
authors: Masaki
authors: M
authors: Unno
authors: Claus W
authors: CW
authors: Heizmann
authors: Yoshinobu
authors: Y
authors: Izumi
mesh_terms: D000495:Allosteric Site; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002412:Cations / Q000378:metabolism; D002942:Circular Dichroism; D018360:Crystallography, X-Ray; D020832:EF Hand Motifs; D006801:Humans; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization; D011994:Recombinant Proteins; D009418:S100 Proteins / Q000737:chemistry* / Q000378:metabolism*; D053838:Scattering, Small Angle; D015032:Zinc / Q000378:metabolism*
chemical_list: D002412:Cations; D011994:Recombinant Proteins; D009418:S100 Proteins; C082356:S100A3 protein, human; D015032:Zinc; D002118:Calcium