title: Identification and characterization of Eimeria tenella apical membrane antigen-1 (AMA1).
abstract: Apical membrane antigen-1 (AMA1) is a micronemal protein of apicomplexan parasites that appears to be essential during the invasion of host cells. In this study, a full-length cDNA of AMA1 was identified from Eimeria tenella (Et) using expressed sequence tag and the rapid amplification of cDNA ends technique. EtAMA1 had an open reading frame of 1608 bp encoding a protein of 535 amino acids. Quantitative real-time PCR analysis revealed that EtAMA1 was expressed at higher levels in sporozoites than in the other developmental stages (unsporulated oocysts, sporulated oocysts and second-generation merozoites). The ectodomain sequence was expressed as recombinant EtAMA1 (rEtAMA1) and rabbit polyclonal antibodies raised against the rEtAMA1 recognized a 58-kDa native parasite protein by Western Blotting and had a potent inhibitory effect on parasite invasion, decreasing it by approximately 70%. Immunofluorescence analysis and immunohistochemistry analysis showed EtAMA1 might play an important role in sporozoite invasion and development.
authors: Key Laboratory for Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Minhang District, Shanghai, China.
authors: Lianlian
authors: L
authors: Jiang
authors: Jiaojiao
authors: J
authors: Lin
authors: Hongyu
authors: H
authors: Han
authors: Hui
authors: H
authors: Dong
authors: Qiping
authors: Q
authors: Zhao
authors: Shunhai
authors: S
authors: Zhu
authors: Bing
authors: B
authors: Huang
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002645:Chickens; D016786:Eimeria tenella / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D060888:Real-Time Polymerase Chain Reaction; D034101:Sporozoites / Q000254:growth & development / Q000378:metabolism
chemical_list: D000953:Antigens, Protozoan