title: Dual-color system for simultaneously monitoring intracellular Ca(2+) and ATP dynamics.
abstract: Although Ca(2+) regulates energy metabolism through diverse pathways, there have been no methods to monitor both Ca(2+) dynamics and metabolic activity simultaneously. Here we report a novel system for simultaneously monitoring intracellular Ca(2+) and ATP levels using a blue-emitting photoprotein and a red-emitting beetle luciferase. Using this system, we monitored the dynamic changes simultaneously in both intracellular Ca(2+) and ATP levels during chondrogenesis. We have found that both intracellular Ca(2+) and ATP levels oscillated and their oscillations have a nearly antiphase relationship with each other. The dual-color monitoring system is useful for studying the relationship between Ca(2+) dynamics and energy metabolic pathways.
authors: Regenerative Medicine/Tissue Engineering Division, Research Center for Cooperative Projects, Hokkaido University Graduate School of Medicine, Sapporo, Japan. kwonhj@med.hokudai.ac.jp
authors: Hyuck Joon
authors: HJ
authors: Kwon
authors: Yoshihiro
authors: Y
authors: Ohmiya
authors: Kazunori
authors: K
authors: Yasuda
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003116:Color; D042541:Intracellular Space / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods*
chemical_list: D000255:Adenosine Triphosphate; D002118:Calcium