title: Creation of human cardiac cell sheets using pluripotent stem cells.
abstract: Although we previously reported the development of cell-dense thickened cardiac tissue by repeated transplantation-based vascularization of neonatal rat cardiac cell sheets, the cell sources for human cardiac cells sheets and their functions have not been fully elucidated. In this study, we developed a bioreactor to expand and induce cardiac differentiation of human induced pluripotent stem cells (hiPSCs). Bioreactor culture for 14 days produced around 8×10(7) cells/100 ml vessel and about 80% of cells were positive for cardiac troponin T. After cardiac differentiation, cardiomyocytes were cultured on temperature-responsive culture dishes and showed spontaneous and synchronous beating, even after cell sheets were detached from culture dishes. Furthermore, extracellular action potential propagation was observed between cell sheets when two cardiac cell sheets were partially overlaid. These findings suggest that cardiac cell sheets formed by hiPSC-derived cardiomyocytes might have sufficient properties for the creation of thickened cardiac tissue.
authors: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo 162-8666, Japan.
authors: Katsuhisa
authors: K
authors: Matsuura
authors: Masanori
authors: M
authors: Wada
authors: Tatsuya
authors: T
authors: Shimizu
authors: Yuji
authors: Y
authors: Haraguchi
authors: Fumiko
authors: F
authors: Sato
authors: Kasumi
authors: K
authors: Sugiyama
authors: Kanako
authors: K
authors: Konishi
authors: Yuji
authors: Y
authors: Shiba
authors: Hinako
authors: H
authors: Ichikawa
authors: Aki
authors: A
authors: Tachibana
authors: Uichi
authors: U
authors: Ikeda
authors: Masayuki
authors: M
authors: Yamato
authors: Nobuhisa
authors: N
authors: Hagiwara
authors: Teruo
authors: T
authors: Okano
mesh_terms: D019149:Bioreactors; D002454:Cell Differentiation; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D032383:Myocytes, Cardiac / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*