title: High expression of human monocyte iNOS mRNA induced by Paracoccidioides brasiliensis is not associated with increase in NO production.
abstract: In this study we investigated the role of nitric oxide (NO) in monocyte fungicidal activity against Paracoccidioides brasiliensis. We found that cells primed with IFN-γ, TNF-α or GM-CSF and challenged with a high-(Pb18) or low-virulence (Pb265) strain of the fungus increase their fungicidal activity. Expression of iNOS mRNA was increased after priming cells with each cytokine, and tended to be inhibited by Pb18. Despite up-regulation of iNOS mRNA expression by Pb265, an equivalent increase in NO production was not detected, as metabolite levels were similar in all cultures. The results indicated that high expression of human monocyte iNOS mRNA induced by P. brasiliensis is not correlated with NO concentrations produced.
authors: UNESP - Univ Estadual Paulista, Instituto de Biociências, Departamento de Microbiologia e Imunologia, Campus de Botucatu, São Paulo, Brazil.
authors: Ana Paula
authors: AP
authors: Bordon-Graciani
authors: Luciane Alarcão
authors: LA
authors: Dias-Melicio
authors: Michele Janegitz
authors: MJ
authors: Acorci-Valério
authors: João Pessoa
authors: JP
authors: Araujo
authors: Angela Maria Victoriano de Campos
authors: AM
authors: Soares
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D050296:Microbial Viability; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology*; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis* / Q000235:genetics; D010228:Paracoccidioides / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D009569:Nitric Oxide; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II