title: Expression of Wnt 3a, β-catenin, cyclin D1 and PCNA in mouse dentate gyrus subgranular zone (SGZ): a possible role of Wnt pathway in SGZ neural stem cell proliferation.
abstract: In mammalian dentate gyrus subgranular           zone, the addition of new neurons throughout adulthood           is a remarkable form of structural plasticity.           Yet, the molecular controls over subgranular zone           neural stem cell proliferation, survival, and differentiation           are poorly understood. In this study we analysed           the expression of Wnt 3a, β-catenin, cyclin D1           and proliferating cell nuclear antigen in mouse subgranular           zone to elucidate the involvement of Wnt           pathway in subgranular zone neural stem cell proliferation.           We performed immunohistochemistry and           RT-PCR for the above molecules on adult and postnatal           developing hippocampal tissues of mice, respectively.           RT-PCR analysis showed a gradual increase           in expression of mRNA of Wnt 3a, β-catenin,           cyclin D1 and proliferating cell nuclear antigen as           the postnatal hippocampus developed, and immunohistochemical           analysis showed a highly positive immunoreactive           expression for Wnt 3a, β-catenin, cyclin           D1 and proliferating cell nuclear antigen in the           subgranular zone cells. Together, our data suggested           that the Wnt pathway is activated in subgranular           zone and could play an important role in regulating           subgranular zone neural stem cell proliferation in           mouse hippocampus.
authors: Unit of Biochemistry, Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu, India.
authors: D U
authors: DU
authors: Kumar
authors: H
authors: H
authors: Devaraj
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D018891:Dentate Gyrus / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000235:genetics / Q000502:physiology; D060509:Wnt3A Protein / Q000235:genetics / Q000378:metabolism*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D060509:Wnt3A Protein; D051176:beta Catenin; D019938:Cyclin D1