title: Involvement of nuclear factor kappa B in high-fat diet-related pancreatic fibrosis in rats.
abstract: OBJECTIVE
  High-fat diets contribute to pancreatic fibrogenesis, but the pathogenesis remains unclear. This study investigated the role of nuclear factor kappa B (NF-κB) in high-fat diet-induced pancreatic fibrosis in rats.
  
  
  METHODS
  Male Wistar rats were fed a high-fat diet or standard normal chow for 20 weeks. Pancreatic fibrosis was determined by Sirius red staining. Immunohistochemical staining, reverse transcription-polymerase chain reaction and Western blotting were used to identify NF-κB-associated genes or protein expressions.
  
  
  RESULTS
  Inflammation, fat deposition, pancreatic stellate cell activation and fibrosis were observed in the pancreases of the high-fat diet group. NF-κB subunit p65 (NF-κB/p65) expression was localized to the nucleus, and intercellular adhesion molecule 1 (ICAM-1) was over-expressed. Pancreatic gene expression levels of NF-κB/p65, ICAM-1 and tumor necrosis factor α were all elevated significantly in rats fed a high-fat diet compared with control rats. Western blotting also revealed significantly increased levels of ICAM-1 and nuclear NF-κB/p65 in rats fed high-fat diets comparison with control rats.
  
  
  CONCLUSIONS
  NF-κB is involved in high-fat diet-related pancreatic fibrosis.
authors: Department of Gastroenterology, Shandong Qianfoshan Hospital, Shandong University School of Medicine, Shandong, China.
authors: Ming-Xian
authors: MX
authors: Yan
authors: Hong-Bo
authors: HB
authors: Ren
authors: Yi
authors: Y
authors: Kou
authors: Min
authors: M
authors: Meng
authors: Yan-Qing
authors: YQ
authors: Li