title: Accurate and rapid species typing from cutaneous and mucocutaneous leishmaniasis lesions of the New World.
abstract: The heat-shock protein 70 gene (hsp70) has been exploited for Leishmania species identification in the Old and New World, using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis. Three new Leishmania-specific hsp70 PCRs were recently described, and we applied 2 of these on 89 clinical samples from a total of 73 Peruvian patients with either cutaneous or mucocutaneous leishmaniasis. The new PCRs on average showed a 2- to 3-fold improved sensitivity in the tested sample types (lesion biopsies, aspirates, and scrapings), for both genus detection and species typing, and were most successful in biopsies. Leishmania braziliensis, L. peruviana, and L. guyanensis were encountered. About one third of the L. braziliensis parasites contained 2 hsp70 alleles. This study is a paradigm for the implementation of a globally applicable upgraded tool for the identification of Leishmania directly on human specimens from cutaneous and mucocutaneous lesions in the New World.
authors: Institute of Tropical Medicine Pedro Kourí, 13600 Havana, Cuba.
authors: Jorge
authors: J
authors: Fraga
authors: Nicolas
authors: N
authors: Veland
authors: Ana M
authors: AM
authors: Montalvo
authors: Nicolas
authors: N
authors: Praet
authors: Andrea K
authors: AK
authors: Boggild
authors: Braulio M
authors: BM
authors: Valencia
authors: Jorge
authors: J
authors: Arévalo
authors: Alejandro
authors: A
authors: Llanos-Cuentas
authors: Jean-Claude
authors: JC
authors: Dujardin
authors: Gert
authors: G
authors: Van der Auwera
mesh_terms: D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007891:Leishmania / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000469:parasitology*; D010568:Peru; D016133:Polymerase Chain Reaction / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics
chemical_list: D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins