title: [Genetic diversity and volatile components of Asarum sieboldii in Qin-ba region].
abstract: OBJECTIVE
  To analyze the genetic diversity and the volatile components of Asarum sieboldii from seven habitats in Qin-ba region.
  
  
  METHODS
  The genetic diversity of the herb was analyzed by ISSR (inter simple sequence repeat) markers; The relative content volatile components of the herb were dectected by head space solid-phase microextraction gas chromatogrphy-mass spectrometry (HS-SPME-GC-MS). The contents of the 3 main components were analyzed by steam distillation gas chromatography-mass spectrometry (GC-MS).
  
  
  RESULTS
  57 bands were amplified from 7 populations by 6 reliable primers, 51 of which were polymorphic (89.47% of the total). The cluster analysis presented that these resources were divided into two main groups. There were differences in the chemical components and the contents of Asarum sieboldii from the 6 wild habitats. Except for some same components, many unique components were identified in them respectively. In addition, some components could be detected only in some populations which had smaller genetic distance.
  
  
  CONCLUSIONS
  Cluster analysis shows no direct correlation between genetic distance and geographic distance of Asarum sieboldii in Qin-ba region. The accumulations of some volatile components of Asarum sieboldii are possibly related to genetic diversity.
authors: Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, College of Life Science, Shaanxi Normal University, Xian 710062, China.
authors: Su-ying
authors: SY
authors: Hu
authors: Gang
authors: G
authors: He
authors: Bei
authors: B
authors: Chen
authors: Lang-jun
authors: LJ
authors: Cui
authors: Jian-xiong
authors: JX
authors: Yang
mesh_terms: D030023:Asarum / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D016000:Cluster Analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D014644:Genetic Variation; D009822:Oils, Volatile / Q000032:analysis* / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D011786:Quality Control; D015203:Reproducibility of Results; D012451:Safrole / Q000032:analysis; D052617:Solid Phase Microextraction / Q000379:methods
chemical_list: D009822:Oils, Volatile; D012451:Safrole