title: Development and comparative assessment of Raman spectroscopic classification algorithms for lesion discrimination in stereotactic breast biopsies with microcalcifications.
abstract: Microcalcifications are an early mammographic sign of breast cancer and a target for stereotactic breast needle biopsy. Here, we develop and compare different approaches for developing Raman classification algorithms to diagnose invasive and in situ breast cancer, fibrocystic change and fibroadenoma that can be associated with microcalcifications. In this study, Raman spectra were acquired from tissue cores obtained from fresh breast biopsies and analyzed using a constituent-based breast model. Diagnostic algorithms based on the breast model fit coefficients were devised using logistic regression, C4.5 decision tree classification, k-nearest neighbor (k -NN) and support vector machine (SVM) analysis, and subjected to leave-one-out cross validation. The best performing algorithm was based on SVM analysis (with radial basis function), which yielded a positive predictive value of 100% and negative predictive value of 96% for cancer diagnosis. Importantly, these results demonstrate that Raman spectroscopy provides adequate diagnostic information for lesion discrimination even in the presence of microcalcifications, which to the best of our knowledge has not been previously reported.
authors: Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, USA. ndingari@mit.edu
authors: Narahara Chari
authors: NC
authors: Dingari
authors: Ishan
authors: I
authors: Barman
authors: Anushree
authors: A
authors: Saha
authors: Sasha
authors: S
authors: McGee
authors: Luis H
authors: LH
authors: Galindo
authors: Wendy
authors: W
authors: Liu
authors: Donna
authors: D
authors: Plecha
authors: Nina
authors: N
authors: Klein
authors: Ramachandra Rao
authors: RR
authors: Dasari
authors: Maryann
authors: M
authors: Fitzmaurice
mesh_terms: D000328:Adult; D000368:Aged; D000465:Algorithms; D062005:Biopsy, Large-Core Needle; D001940:Breast / Q000473:pathology*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000473:pathology; D002114:Calcinosis / Q000473:pathology*; D005260:Female; D005557:Formaldehyde / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D016612:Paraffin Embedding; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013238:Stereotaxic Techniques
chemical_list: D005557:Formaldehyde