title: Reduction in cholesterol absorption in Caco-2 cells through the down-regulation of Niemann-Pick C1-like 1 by the putative probiotic strains Lactobacillus rhamnosus BFE5264 and Lactobacillus plantarum NR74 from fermented foods.
abstract: Hypercholesterolaemia is a major risk factor related to atherosclerosis, and it may be influenced by our diet. This study addresses the impact of Lactobacillus rhamnosus BFE5264 (isolated from Maasai fermented milk) and Lactobacillus plantarum NR74 (from Korean kimchi) on the control of cholesterol absorption through down-regulation of Niemann-Pick C1-like 1 (NPC1L1) expression. Caco-2 enterocytes were treated with the live, heat-killed (HK) bacteria, bacterial cell wall extracts and metabolites; mRNA level and protein expression were measured. Caco-2 cells showed lower NPC1L1 expression in the presence of the live test strains than the control, elucidating down-regulation of cholesterol uptake, and were compared well with the positive control, L. rhamnosus GG. This effect was also observed with HK bacteria and cell wall fractions but not with their metabolites. The potential of some Lactobacillus strains associated with traditional fermented foods to suppress cholesterol uptake and promote its efflux in enterocytes has been suggested from these data.
authors: School of Life Sciences, Handong Global University, Pohang, Gyeongbuk, Republic of Korea.
authors: Hong-Sup
authors: HS
authors: Yoon
authors: Jae-Hyun
authors: JH
authors: Ju
authors: Han-Nah
authors: HN
authors: Kim
authors: Hyun-Joon
authors: HJ
authors: Park
authors: Yosep
authors: Y
authors: Ji
authors: Ji-Eun
authors: JE
authors: Lee
authors: Hyeun-Kil
authors: HK
authors: Shin
authors: Myoung-Sool
authors: MS
authors: Do
authors: Wilhelm
authors: W
authors: Holzapfel
mesh_terms: D000924:Anticholesteremic Agents / Q000627:therapeutic use; D018938:Caco-2 Cells; D002473:Cell Wall; D002784:Cholesterol / Q000378:metabolism*; D004032:Diet; D015536:Down-Regulation; D020895:Enterocytes / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D006358:Hot Temperature; D006801:Humans; D006937:Hypercholesterolemia / Q000378:metabolism* / Q000517:prevention & control; D007408:Intestinal Absorption; D048191:Lactiplantibacillus plantarum / Q000302:isolation & purification / Q000378:metabolism; D052201:Lacticaseibacillus rhamnosus / Q000302:isolation & purification / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D026901:Membrane Transport Proteins; D019936:Probiotics / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000924:Anticholesteremic Agents; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; C410800:NPC1L1 protein, human; D012333:RNA, Messenger; D002784:Cholesterol