title: Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein.
abstract: Population signals from neuronal ensembles in cortex during behavior are commonly measured with EEG, local field potential (LFP), and voltage-sensitive dyes. A genetically encoded voltage indicator would be useful for detection of such signals in specific cell types. Here we describe how this goal can be achieved with Butterfly, a voltage-sensitive fluorescent protein (VSFP) with a subthreshold detection range and enhancements designed for voltage imaging from single neurons to brain in vivo. VSFP-Butterfly showed reliable membrane targeting, maximum response gain around standard neuronal resting membrane potential, fast kinetics for single-cell synaptic responses, and a high signal-to-noise ratio. Butterfly reports excitatory postsynaptic potentials (EPSPs) in cortical neurons, whisker-evoked responses in barrel cortex, 25-Hz gamma oscillations in hippocampal slices, and 2- to 12-Hz slow waves during brain state modulation in vivo. Our findings demonstrate that cell class-specific voltage imaging is practical with VSFP-Butterfly, and expand the genetic toolbox for the detection of neuronal population dynamics.
authors: RIKEN Brain Science Institute, Wako City, Saitama, Japan.
authors: Walther
authors: W
authors: Akemann
authors: Hiroki
authors: H
authors: Mutoh
authors: Amélie
authors: A
authors: Perron
authors: Yun Kyung
authors: YK
authors: Park
authors: Yuka
authors: Y
authors: Iwamoto
authors: Thomas
authors: T
authors: Knöpfel
mesh_terms: D000818:Animals; D058256:Brain Waves; D002540:Cerebral Cortex / Q000166:cytology / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials; D031541:Fluorescence Resonance Energy Transfer; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009474:Neurons / Q000145:classification / Q000502:physiology*; D062308:Optogenetics; D016716:PC12 Cells; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry; D017434:Protein Structure, Tertiary; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D056969:Voltage-Sensitive Dye Imaging / Q000379:methods*
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; C509671:voltage-sensor-containing phosphatase, Ciona intestinalis; D010744:Phosphoric Monoester Hydrolases