title: Fluorescent silica nanoparticles improve optical imaging of stem cells allowing direct discrimination between live and early-stage apoptotic cells.
abstract: Highly bright and photostable cyanine dye-doped silica nanoparticles, IRIS Dots, are developed, which can efficiently label human mesenchymal stem cells (hMSCs). The application procedure used to label hMSCs is fast (2 h), the concentration of IRIS Dots for efficient labeling is low (20 μg mL(-1) ), and the labeled cells can be visualized by flow cytometry, confocal microscopy, and transmission electron microscopy. Labeled hMSCs are unaffected in their viability and proliferation, as well as stemness surface marker expression and differentiation capability into osteocytes. Moreover, this is the first report that shows nonfunctionalized IRIS Dots can discriminate between live and early-stage apoptotic stem cells (both mesenchymal and embryonic) through a distinct external cell surface distribution. On the basis of biocompatibility, efficient labeling, and apoptotic discrimination potential, it is suggested that IRIS Dots can serve as a promising stem cell tracking agent.
authors: Department of Clinical and Biological Sciences, University of Turin, Regione Gonzole 10, 10043 Orbassano, Torino, Italy.
authors: Lisa
authors: L
authors: Accomasso
authors: Elisa
authors: E
authors: Cibrario Rocchietti
authors: Stefania
authors: S
authors: Raimondo
authors: Federico
authors: F
authors: Catalano
authors: Gabriele
authors: G
authors: Alberto
authors: Andrea
authors: A
authors: Giannitti
authors: Valentina
authors: V
authors: Minieri
authors: Valentina
authors: V
authors: Turinetto
authors: Luca
authors: L
authors: Orlando
authors: Silvia
authors: S
authors: Saviozzi
authors: Giuseppe
authors: G
authors: Caputo
authors: Stefano
authors: S
authors: Geuna
authors: Gianmario
authors: G
authors: Martra
authors: Claudia
authors: C
authors: Giachino
mesh_terms: D017209:Apoptosis / Q000502:physiology; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry*; D013234:Stem Cells
chemical_list: D012822:Silicon Dioxide