title: Higher apoptosis index and proliferation index in colonocytes of patients with ulcerative colitis in remission.
abstract: Ulcerative colitis (UC) is a inflammatory disease of large bowel. The amount of people suffering from UC increases from year to year. Pathogenesis of this affection is still not entirely clear. Mechanisms of proliferation and apoptosis in colonocytes in the course of the disease are defected.
  
  
  OBJECTIVE
  was to assess the rate of proliferation and intensity of apoptosis in colonocytes in patients with diagnose UC.
  
  
  METHODS
  Colon pathological samples taken from patients with diagnosed ulceraive colitis were examined. Patients were in both clinical and endoscopic remission and were treated with mesalazin. They were patient of Department of General and Colorectal Surgery. To estimate proliferation index dye with monoclonal antibody against Ki67. To determine apoptosis level immunohistochemistry with antybody against Bax was used.
  
  
  RESULTS
  Average Ki-67 in the test group was 42,13%, the largest value amounted to 57% and the lowest of 33%. Average value of Bax was 1.47 and ranged between 0-3. High index of bax appear not only in the bottom of the crypt, but also at their outlet.
  
  
  CONCLUSIONS
  In ulecerative colitis genetic and immunological disturbances occur despite treatment. Mesalazine acting only on certain routes associated with the UC holds the remission, without, however "the molecular remission". Thus, it appears that the results of our research are another proof of the necessary caution in weaning support treatment.
authors: Department of General and Colorectal Surgery, Medical University in Łódź, Poland.
authors: Jarosław
authors: J
authors: Buczyński
authors: Michał
authors: M
authors: Spychalski
authors: Agnieszka
authors: A
authors: Ławska-Wierzchniewska
authors: Adam
authors: A
authors: Dziki
mesh_terms: D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage; D017209:Apoptosis; D049109:Cell Proliferation / Q000187:drug effects; D003093:Colitis, Ulcerative / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology*; D003106:Colon / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D019804:Mesalamine / Q000008:administration & dosage; D008875:Middle Aged; D011044:Poland; D012074:Remission Induction; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D019394:Ki-67 Antigen; D051028:bcl-2-Associated X Protein; D019804:Mesalamine