title: Adult hematopoietic progenitors are multipotent in chimeric mice.
abstract: Embryonic stem cells (ESCs) and adult somatic cells, induced to pluripotency (iPSCs), can differentiate into multiple cell lineages. We previously reported that adult mammalian bone marrow contains a sub-population of CD34+ cells that express genes of ESCs and genes required to generate iPSCs. They also express lineage genes of the three embryonic germ layers. Are these CD34+ cells multipotent? Here, CD34+ bone marrow stem cells from adult male ROSA mice, which carry two markers: the β-galactosidase gene and the male Y chromosome, were transplanted into blastocysts of wildtype mice. Each female ROSA chimera generated had a distinct pattern of male-derived organs expressing β-galactosidase; e.g., ectodermal brain, dorsal root ganglia and skin; mesodermal heart, bone and bone marrow; and endodermal pancreas, intestine, and liver. Thus, adult mammals carry cells that appear to exhibit a developmental potential reminiscent of ESCs and iPSCs suggesting they could be used for cell replacement therapy.
authors: CNRS UMR, Université Paris Descartes, France. bernard.pessac@wanadoo.fr
authors: Bernard
authors: B
authors: Pessac
authors: Vamshi K
authors: VK
authors: Nimmagadda
authors: Tapas
authors: T
authors: Makar
authors: Paul S
authors: PS
authors: Fishman
authors: Christopher T
authors: CT
authors: Bever
authors: David
authors: D
authors: Trisler
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D019070:Cell Lineage; D004624:Embryo Transfer; D005260:Female; D017930:Genes, Reporter; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D039902:Multipotent Stem Cells / Q000166:cytology*; D009928:Organ Specificity; D038081:Organogenesis; D018183:Transplantation Chimera; D014998:Y Chromosome; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
chemical_list: D018952:Antigens, CD34; D001616:beta-Galactosidase