title: The toolbox of vesicle sidedness determination.
abstract: Vesicles prepared from cellular plasma membranes are widely used in science for different purposes. The outer membrane leaflet differs from the inner membrane leaflet of the vesicle, and during vesicle preparation procedures two types of vesicles will be generated: right-side-out vesicles, of which the outer leaflet is topologically equivalent to the outer monolayer of the cellular plasma membrane, and inside-out vesicles. Because two populations of vesicles exist, sidedness information of the vesicle preparation is indispensable. This note focuses on the ins and outs of sidedness determination of vesicles and compares various methodologies used to establish this ratio.
authors: Department of Cell Biology, University Medical Center Groningen, University of Groningen, AV Groningen, The Netherlands.
authors: Peter
authors: P
authors: Meszaros
authors: Dick
authors: D
authors: Hoekstra
authors: Jan Willem
authors: JW
authors: Kok
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000110:Acetylcholinesterase / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D011312:Pressure; D012794:Sialic Acids / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D012794:Sialic Acids; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); D009247:NADH, NADPH Oxidoreductases; D000110:Acetylcholinesterase; D015720:5'-Nucleotidase; D000254:Sodium-Potassium-Exchanging ATPase