title: Xenogeneic protein-free cultivation of mesenchymal stromal cells - towards clinical applications.
abstract: We have studied a rapid cultivation method           for human mesenchymal stromal cells based on           CellGroTM medium and human serum, supplemented           with insulin, ascorbic acid, dexamethasone, epidermal           growth factor, platelet-derived growth factor           BB, macrophage colony-stimulating factor and fibroblast           growth factor 2. This study has shown that           rapid expansion of human multipotent mesenchymal           stromal cells using human serum could not be           achieved without addition of growth factors. Furthermore,           we have found that insulin and, quite           probably, epidermal growth factor may be omitted           from our formula without loss of colony-forming           capacity           or total cell yield. On the other hand, dexamethasone,           ascorbic acid and fibroblast growth factor           2 were necessary for the growth and colony-forming           capacity of multipotent mesenchymal stromal           cells, while platelet-derived growth factor BB prevented           their differentiation into adipogenic lineage.           Moreover, multipotent mesenchymal stromal cells           cultivated in our system expressed higher levels of           bone morphogenetic protein 2, but not bone morphogenetic           protein 7, than cells cultivated in α-MEM           with foetal bovine serum. This shows that our system promotes differentiation of mesenchymal cells towards           osteogenic and chondrogenic lineages, making           them more suitable for bone and cartilage engineering           than cells grown in conventional media. Furthermore,           we have proved that these cells may be conveniently           cultivated in a closed system, in vessels           certified for clinical use (RoboFlaskTM), making the           transfer of our cultivation technology to good clinical           practice easier and more convenient.
authors: Department of Children and Adult Orthopedic Surgery, Second Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. stehlikd@seznam.cz
authors: D
authors: D
authors: Stehlík
authors: R
authors: R
authors: Pytlík
authors: H
authors: H
authors: Rychtrmocová
authors: L
authors: L
authors: Kideryová
authors: R
authors: R
authors: Veselá
authors: Z
authors: Z
authors: Kopečný
authors: T
authors: T
authors: Trč
authors: M
authors: M
authors: Trněný
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism
chemical_list: D055396:Bone Morphogenetic Protein 2; D055419:Bone Morphogenetic Protein 7; D015675:Osteocalcin