title: Functional divergence of gene duplicates - a domain-centric view.
abstract: BACKGROUND
  Gene duplicates have been shown to evolve at different rates. Here we further investigate the mechanism and functional underpinning of this phenomenon by assessing asymmetric evolution specifically within functional domains of gene duplicates.
  
  
  RESULTS
  Based on duplicate genes in five teleost fishes resulting from a whole genome duplication event, we first show that a Fisher Exact test based approach to detect asymmetry is more sensitive than the previously used Likelihood Ratio test. Using our Fisher Exact test, we found that the evolutionary rate asymmetry in the overall protein is largely explained by the asymmetric evolution within specific protein domains. Moreover, among cases of asymmetrically evolving domains, for the gene copy containing a fast evolving domain, the non-synonymous substitutions often cluster within the fast evolving domain. We found that rare substitutions were preferred within asymmetrically evolving domains suggestive of functional divergence. While overall ~32 % of the domains tested were found to be evolving asymmetrically, certain protein domains such as the Tyrosine and Ser/Thr Kinase domains had a much greater prevalence of asymmetric evolution. Finally, based on the spatial expression of Zebra fish duplicate proteins during development, we found that protein pairs containing asymmetrically evolving domains had a greater divergence in gene expression as compared to the duplicate proteins that did not exhibit asymmetric evolution.
  
  
  CONCLUSIONS
  Taken together, our results suggest that the previously observed asymmetry in the overall duplicate protein evolution is largely due to divergence of specific domains of the protein, and coincides with divergence in spatial expression domains.
authors: Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
authors: Mugdha
authors: M
authors: Khaladkar
authors: Sridhar
authors: S
authors: Hannenhalli
mesh_terms: D000818:Animals; D019295:Computational Biology; D019143:Evolution, Molecular; D005399:Fishes / Q000235:genetics*; D020131:Genes, Duplicate; D016013:Likelihood Functions; D051379:Mice; D015233:Models, Statistical; D017434:Protein Structure, Tertiary