title: Development and validation of a rapid RP-HPLC method for analysis of (-)-copalic acid in copaíba oleoresin.
abstract: The Copaifera species (Leguminoseae) are popularly known as 'copaíba' or 'copaíva' and are grown in the states of Amazonas, Pará and Ceará in northern Brazil. The oleoresins obtained from these species have been extensively used owing to their pharmacological potential and their application in cosmetic and pharmaceutical preparations. In the present study, the development and validation of a novel, rapid and efficient RP-HPLC methodology for the analysis of the diterpene (-)-copalic acid (CA), pointed out as the only chemical marker of the Copaifera genus, are described. The regression equation (Y = 26,707x - 29,498) was obtained with good linearity (r(2) = 0.9993) and the limits of quantification and detection were 9.182 and 3.032 µg/mL, respectively. The precision and the accuracy of the method were adequate (lower than 4%). Finally, the validation parameters evaluated were satisfactorily met, so the developed method represents a suitable tool for application in the quality control of such natural products. Further studies aiming to develop analytical methodologies for each Copaifera species using a more representative number of chemical markers should be performed.
authors: Núcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, Av. Dr Armando Salles de Oliveira 201, Franca, 14404-600, SP, Brazil.
authors: Ariana Borges
authors: AB
authors: Souza
authors: Monique Rodrigues
authors: MR
authors: Moreira
authors: Carly Henrique Gambeta
authors: CH
authors: Borges
authors: Marília Rodrigues
authors: MR
authors: Simão
authors: Jairo Kenupp
authors: JK
authors: Bastos
authors: João Paulo Barreto
authors: JP
authors: de Sousa
authors: Sérgio Ricardo
authors: SR
authors: Ambrosio
authors: Rodrigo Cassio Sola
authors: RC
authors: Veneziani
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D004224:Diterpenes / Q000032:analysis* / Q000737:chemistry; D016014:Linear Models; D028321:Plant Preparations / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D004224:Diterpenes; D028321:Plant Preparations; C569239:copaiba oleoresin; C556511:copalic acid