title: Cell perturbation screens for target identification by RNAi.
abstract: Over the last decade, cell-based screening has become a powerful method in target identification and plays an important role both in basic research and drug discovery. The availability of whole genome sequences and improvements in cell-based screening techniques opened new avenues for high-throughput experiments. Large libraries of RNA interference reagents available for many organisms allow the dissection of broad spectrum of cellular processes. Here, we describe the current state of the large-scale phenotype screening with a focus on cell-based screens. We underline the importance and provide details of screen design, scalability, performance, data analysis, and hit prioritization. Similar to classical high-throughput in vitro screens with defined-target approaches in the past, cell-based screens depend on a successful establishment of robust phenotypic assays, the ability to quantitatively measure phenotypic changes and bioinformatics methods for data analysis, integration, and interpretation.
authors: Division of Signaling and Functional Genomics, Department for Cell and Molecular Biology, German Cancer Research Center (DKFZ), Heidelberg University, Heidelberg, Germany.
authors: Kubilay
authors: K
authors: Demir
authors: Michael
authors: M
authors: Boutros
mesh_terms: D000818:Animals; D002477:Cells / Q000378:metabolism*; D055808:Drug Discovery / Q000379:methods*; D015723:Gene Library; D017930:Genes, Reporter / Q000235:genetics; D006801:Humans; D058990:Molecular Targeted Therapy; D010641:Phenotype; D034622:RNA Interference