title: [Production of purified human recombinant alpha2b-interferon].
abstract: Technology for producing biologically active recombinant alpha2b-interferon is based on creating a bacterial producer strain containing the cDNA of human interferon alpha. The authors have obtained two producers of recombinant alpha2b-interferon, the synthesis of the target protein in them occurs in the inclusion bodies. The schemes of isolation and purification of biologically active recombinant alpha2b-interferon have been developed. The drug purity was approximately 97-98%. Biological activity in the culture of sensory cells in the cytopathic test was 4.2*10(8)ME/mg.
authors: A I
authors: AI
authors: Mel'nik
authors: I V
authors: IV
authors: Orlovskaia
authors: N M
authors: NM
authors: Zholobak
authors: N Ia
authors: NIa
authors: Spivak
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000821:virology; D000818:Animals; D002460:Cell Line; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins