title: microrna expression signature of gastric cancer cells relative to normal             gastric mucosa.
abstract: micrornas (miRNAs) play an important role in a wide range of physiological             and developmental processes by negatively regulating the expression of target             genes at the post-transcriptional level. In this study, we investigated the differential             miRNA expression signature between gastric cancer cells and normal gastric mucosa             to determine changes in miRNA expression during gastric cancer development. We             analyzed the global miRNA expression profiles of 9 gastric cancer cell lines and             6 normal gastric mucosa lines using miRNA microarrays. In addition, we performed             quantitative real-time PCR (Q-PCR) to validate the results. Correlations between             the miRNA expression profile and tumor clinicopathological parameters were analyzed.             We found that 17 miRNAs were upregulated in gastric cancer cell lines and 146             miRNAs were downregulated compared to normal gastric mucosa. Using microarray             data and Q-PCR validation, 15 miRNAs were finally selected. These candidate miRNAs             were associated with gastric cancer clinicopathology to various degrees. High             expression levels of hsa-miR-93 were found to predict poor survival (median, 16             vs. 40 months; log-rank test p<0.05). These findings suggest that miRNAs play             vital roles in human gastric cancer. The findings may also provide clues toward             understanding the molecular functions of miRNAs in various biological processes.
authors: Shanghai Key Laboratory of Gastric Neoplasms, Department of Surgery, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
authors: Bei-Qin
authors: BQ
authors: Yu
authors: Li-Ping
authors: LP
authors: Su
authors: Jian-Fang
authors: JF
authors: Li
authors: Qu
authors: Q
authors: Cai
authors: Min
authors: M
authors: Yan
authors: Xue-Hua
authors: XH
authors: Chen
authors: Ying-Yan
authors: YY
authors: Yu
authors: Qin-Long
authors: QL
authors: Gu
authors: Zheng-Gang
authors: ZG
authors: Zhu
authors: Bing-Ya
authors: BY
authors: Liu
mesh_terms: D002460:Cell Line; D019295:Computational Biology; D005753:Gastric Mucosa / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D053208:Kaplan-Meier Estimate; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology
chemical_list: C543350:MIRN93 microRNA, human; D035683:MicroRNAs