title: Membrane protein distribution in composite polymer-lipid thin films.
abstract: We present a model system to demonstrate that the positioning of biomolecules (membrane proteins) in a nonnative, complex thin film environment can be regulated by the phase behavior of film components. Partial separation between an amphiphilic polymer and a lipid drives the protein to a fluid phase, mechanically more similar to a cellular bilayer.
authors: Swiss Nanoscience Institute, University of Basel, Klingelbergstr. 82, 4056 Basel, Switzerland.
authors: Johannes
authors: J
authors: Thoma
authors: Serena
authors: S
authors: Belegrinou
authors: Peggy
authors: P
authors: Rossbach
authors: Mariusz
authors: M
authors: Grzelakowski
authors: Katarzyna
authors: K
authors: Kita-Tokarczyk
authors: Wolfgang
authors: W
authors: Meier
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D005456:Fluorescent Dyes; D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010080:Oxazoles / Q000737:chemistry*; D044367:Phase Transition; D011108:Polymers / Q000737:chemistry*; D018272:Porins / Q000737:chemistry*; D013388:Succinimides; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: C492694:Alexa Fluor 488 carboxylic acid succinimidyl ester; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008565:Membrane Proteins; C109237:OmpF protein; D010080:Oxazoles; D011108:Polymers; D018272:Porins; D013388:Succinimides; D013501:Surface-Active Agents; D015060:1,2-Dipalmitoylphosphatidylcholine; C081581:colfosceril palmitate