title: Expression of vitamin D-metabolizing enzymes in human adipose tissue -- the effect of obesity and diet-induced weight loss.
abstract: OBJECTIVE
  Low vitamin D (VD) levels are common in obesity. We hypothesized that this may be due to metabolism of VD in adipose tissue (AT). Thus, we studied (1) whether the VD-metabolizing enzymes were expressed differently in AT of lean and obese individuals and in visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT), and (2) whether their expression was influenced by weight loss.
  
  
  METHODS
  Samples of SAT and VAT were analyzed for expression of the vitamin-D-25-hydroxylases CYP2R1, CYP2J2, CYP27A1 and CYP3A4, the 25-vitamin-D-1α-hydroxylase CYP27B1, the catabolic vitamin-D-24-hydroxylase CYP24A1, and the vitamin D receptor, using reverse transcriptase-PCR. Moreover, plasma 25-hydroxy-vitamin D (25OHD) level was measured and related to the expression of these enzymes. Samples of SAT and VAT from 20 lean women and 20 obese women, and samples of SAT from 17 obese subjects before and after a 10% weight loss were analyzed.
  
  
  RESULTS
  A plasma 25OHD level <50 nmol l(-1) was highly prevalent in both lean (45%) and obese (90%) women (P<0.01). Plasma 25OHD increased by 27% after weight loss in the obese individuals (P<0.05). Expression levels of the 25-hydroxylase CYP2J2 and the 1α-hydroxylase CYP27B1 were decreased by 71% (P<0.0001) and 49% (P<0.05), respectively, in SAT of the obese. CYP24A1 did not differ between lean and obese women, but the expression was increased by 79% (P<0.05) after weight loss.
  
  
  CONCLUSIONS
  Obesity is characterized by a decreased expression of the 25-hydroxylase CYP2J2 and the 1α-hydroxylase CYP27B1 in SAT, whereas the catabolic CYP24A1 does not differ between lean and obese women. However, the expression of CYP24A1 is increased after weight loss. Accordingly, AT has the capacity to metabolize VD locally, and this can be dynamically altered during obesity and weight loss.
authors: Department of Endocrinology and Internal Medicine MEA, Aarhus University Hospital, Aarhus, Denmark. louisewamberg@dadlnet.dk
authors: L
authors: L
authors: Wamberg
authors: T
authors: T
authors: Christiansen
authors: S K
authors: SK
authors: Paulsen
authors: S
authors: S
authors: Fisker
authors: P
authors: P
authors: Rask
authors: L
authors: L
authors: Rejnmark
authors: B
authors: B
authors: Richelsen
authors: S B
authors: SB
authors: Pedersen
mesh_terms: D000328:Adult; D003430:Cross-Sectional Studies; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004038:Diet, Reducing; D005260:Female; D006801:Humans; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D008875:Middle Aged; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018167:Receptors, Calcitriol / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism*; D013851:Thinness / Q000378:metabolism*; D014807:Vitamin D / Q000378:metabolism*; D014808:Vitamin D Deficiency / Q000201:enzymology* / Q000209:etiology; D015431:Weight Loss
chemical_list: D012333:RNA, Messenger; D018167:Receptors, Calcitriol; D014807:Vitamin D; D003577:Cytochrome P-450 Enzyme System