title: Mycobacterium iranicum sp. nov., a rapidly growing scotochromogenic species isolated from clinical specimens on three different continents.
abstract: The isolation and characterization of a novel, rapidly growing, scotochromogenic mycobacterial species is reported. Eight independent strains were isolated from clinical specimens from six different countries of the world, two in Iran, two in Italy and one in each of following countries: Greece, The Netherlands, Sweden and the USA. Interestingly, two of the strains were isolated from cerebrospinal fluid. The strains were characterized by rapid growth and presented orange-pigmented scotochromogenic colonies. DNA-based analysis revealed unique sequences in the four regions investigated: the 16S rRNA gene, the rRNA gene internal transcribed spacer 1 and the genes encoding the 65 kDa heat-shock protein and the beta-subunit of RNA polymerase. The phylogenetic analysis placed the strains among the rapidly growing mycobacteria, being most closely related to Mycobacterium gilvum. The genotypic and phenotypic data both strongly supported the inclusion of the strains investigated here as members of a novel species within the genus Mycobacterium; the name Mycobacterium iranicum sp. nov. is proposed to indicate the isolation in Iran of the first recognized strains. The type strain is M05(T) ( = DSM 45541(T) = CCUG 62053(T) = JCM 17461(T)).
authors: Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Hasan
authors: H
authors: Shojaei
authors: Department of Mycobacterial and Respiratory Infections, National Jewish Health, Denver, CO, USA.
authors: Charles
authors: C
authors: Daley
authors: Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, University Hospital of Heraklion, Heraklion, Greece.
authors: Zoe
authors: Z
authors: Gitti
authors: Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Abodolrazagh
authors: A
authors: Hashemi
authors: Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Parvin
authors: P
authors: Heidarieh
authors: Department of Infectious Disease, Culture Collection University of Gothenburg (CCUG), Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden.
authors: Edward R B
authors: ERB
authors: Moore
authors: Isfahan University of Medical Sciences, Isfahan, Iran.
authors: Abass Daei
authors: AD
authors: Naser
authors: Virology Laboratory, Bambino Gesù Children's Hospital, Rome, Italy.
authors: Cristina
authors: C
authors: Russo
authors: Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
authors: Jakko
authors: J
authors: van Ingen
authors: Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy.
authors: Enrico
authors: E
authors: Tortoli
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015373:Bacterial Typing Techniques; D002555:Cerebrospinal Fluid / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D006115:Greece; D006360:Heat-Shock Proteins / Q000235:genetics; D006801:Humans; D007492:Iran; D007558:Italy; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000382:microbiology; D009171:Mycolic Acids / Q000032:analysis; D009426:Netherlands; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013183:Sputum / Q000382:microbiology; D013548:Sweden; D014481:United States; D014947:Wounds and Injuries / Q000382:microbiology; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D005227:Fatty Acids; D006360:Heat-Shock Proteins; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit