title: Biotransformation of 2,6-diaminopurine nucleosides by immobilized Geobacillus stearothermophilus.
abstract: An efficient and green bioprocess to obtain 2,6-diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6-diaminopurine-2'-deoxyriboside and 2,6-diaminopurine riboside, respectively. The selected biocatalyst was successfully stabilized in an agarose matrix and used to produce up to 23.4 g of 2,6-diaminopurine-2'-deoxyriboside in 240 h of process. These nucleoside analogues can be used as prodrug precursors or in antisense oligonucleotide synthesis.
authors: Laboratorio de Investigaciones en Biotecnología Sustentable, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal B1868BXD, Argentina.
authors: Eliana C
authors: EC
authors: De Benedetti
authors: Cintia W
authors: CW
authors: Rivero
authors: Claudia N
authors: CN
authors: Britos
authors: Mario E
authors: ME
authors: Lozano
authors: Jorge A
authors: JA
authors: Trelles
mesh_terms: D015075:2-Aminopurine / Q000031:analogs & derivatives* / Q000378:metabolism; D001711:Biotransformation; D018914:Cells, Immobilized / Q000378:metabolism; D001411:Geobacillus stearothermophilus / Q000378:metabolism*; D009705:Nucleosides / Q000378:metabolism*
chemical_list: D009705:Nucleosides; D015075:2-Aminopurine; C007300:2,6-diaminopurine