title: Errors in ribosomal sequence datasets generated using PCR-coupled 'panbacterial' pyrosequencing, and the establishment of an improved approach.
abstract: Universal bacterial primers are often used in PCR-coupled sequencing approaches to investigate environmental and host-associated bacterial communities. Some of these primers can also amplify eukaryotic DNA. This is leading to the submission of datasets to public databases which are erroneously annotated as prokaryotic sequences. The present note sends a message about the risk of submitting incorrectly annotated sequence data and suggests a reliable approach for the sequencing of 16S rRNA genes and identification of bacteria within complex communities.
authors: BTBS, Università degli Studi di Milano-Bicocca, Piazza della Scienza, 2, 20126 Milano, Italy.
authors: Erica M
authors: EM
authors: Prosdocimi
authors: Stefano
authors: S
authors: Novati
authors: Raffaele
authors: R
authors: Bruno
authors: Claudio
authors: C
authors: Bandi
authors: Patrizia
authors: P
authors: Mulatto
authors: Riccardo
authors: R
authors: Giannico
authors: Maurizio
authors: M
authors: Casiraghi
authors: Emanuele
authors: E
authors: Ferri
mesh_terms: D016477:Artifacts; D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012270:Ribosomes / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S