title: A graphene-based sensor array for high-precision and adaptive target identification with ensemble aptamers.
abstract: In this work, we report a new concept of adaptive "ensemble aptamers" (ENSaptamers) that exploits the collective recognition abilities of a small set of rationally designed, nonspecific DNA sequences to identify molecular or cellular targets discriminatively. In contrast to in vitro-selected aptamers, which possess specific "lock-and-key" recognition, ENSaptamers rely on pattern recognition that mimics natural olfactory or gustatory systems. Nanographene oxide was employed to provide a low-background and highly reproducible fluorescent assay system. We demonstrate that this platform provides a highly discriminative and adaptive tool for high-precision identification of a wide range of targets for diagnostic and proteomic applications with a nearly unlimited supply of ENSaptamer receptors.
authors: Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors: Hao
authors: H
authors: Pei
authors: Jiang
authors: J
authors: Li
authors: Min
authors: M
authors: Lv
authors: Jingyan
authors: J
authors: Wang
authors: Jimin
authors: J
authors: Gao
authors: Jianxin
authors: J
authors: Lu
authors: Yongping
authors: Y
authors: Li
authors: Qing
authors: Q
authors: Huang
authors: Jun
authors: J
authors: Hu
authors: Chunhai
authors: C
authors: Fan
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001419:Bacteria / Q000302:isolation & purification; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods; D001798:Blood Proteins / Q000032:analysis; D002460:Cell Line; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods; D013816:Thermodynamics
chemical_list: D052157:Aptamers, Nucleotide; D001798:Blood Proteins; D005456:Fluorescent Dyes; D011506:Proteins; D006108:Graphite