title: Recognition of CD1d-sulfatide mediated by a type II natural killer T cell antigen receptor.
abstract: Natural killer T cells (NKT cells) are divided into type I and type II subsets on the basis of differences in their T cell antigen receptor (TCR) repertoire and CD1d-antigen specificity. Although the mode by which type I NKT cell TCRs recognize CD1d-antigen has been established, how type II NKT cell TCRs engage CD1d-antigen is unknown. Here we provide a basis for how a type II NKT cell TCR, XV19, recognized CD1d-sulfatide. The XV19 TCR bound orthogonally above the A' pocket of CD1d, in contrast to the parallel docking of type I NKT cell TCRs over the F' pocket of CD1d. At the XV19 TCR-CD1d-sulfatide interface, the TCRα and TCRβ chains sat centrally on CD1d, where the malleable CDR3 loops dominated interactions with CD1d-sulfatide. Accordingly, we highlight the diverse mechanisms by which NKT cell TCRs can bind CD1d and account for the distinct antigen specificity of type II NKT cells.
authors: 1] Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Australia.
authors: Onisha
authors: O
authors: Patel
authors: Daniel G
authors: DG
authors: Pellicci
authors: Stephanie
authors: S
authors: Gras
authors: Maria L
authors: ML
authors: Sandoval-Romero
authors: Adam P
authors: AP
authors: Uldrich
authors: Thierry
authors: T
authors: Mallevaey
authors: Andrew J
authors: AJ
authors: Clarke
authors: Jérôme
authors: J
authors: Le Nours
authors: Alex
authors: A
authors: Theodossis
authors: Susanna L
authors: SL
authors: Cardell
authors: Laurent
authors: L
authors: Gapin
authors: Dale I
authors: DI
authors: Godfrey
authors: Jamie
authors: J
authors: Rossjohn
mesh_terms: D000818:Animals; D055610:Antigens, CD1d / Q000737:chemistry / Q000276:immunology*; D003460:Crystallization; D007694:Killer Cells, Natural / Q000737:chemistry / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D016133:Polymerase Chain Reaction; D020836:Protein Structure, Quaternary; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry* / Q000276:immunology; D013433:Sulfoglycosphingolipids / Q000737:chemistry / Q000276:immunology*; D020349:Surface Plasmon Resonance; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology*
chemical_list: D055610:Antigens, CD1d; C526879:Cd1d1 protein, mouse; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013433:Sulfoglycosphingolipids