title: [Stimulation in vitro of oocytes of acipenserids with progesterone and homologous gonadotropic hormone of hypophysis].
abstract: We showed that the percentage of oocytes of acipenserids ovulating in vitro in Ringer solution modified for sturgeons (RMS) considerably depends on the concentration of sodium bicarbonate and the concentration of progesterone. Under optimal conditions (0.5 g/L of sodium bicarbonate and 30 ng/mL of progesterone), it can be higher than 80. Oocytes that matured and ovulated under such conditions are capable of normal development. In the best case, approximately 70% of developing embryos (of the number of ovulated oocytes) reach the stage of hatching (dead-line of observation). This method of producing offspring based on the insemination of oocytes that have matured and ovulated in vitro can be used in work with single females of rare and disappearing species of acipenserids.
authors: M N
authors: MN
authors: Skoblina
authors: B F
authors: BF
authors: Goncharov
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D005307:Fertilization in Vitro; D005399:Fishes; D006065:Gonadotropins, Pituitary / Q000494:pharmacology*; D008297:Male; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology*; D010060:Ovulation / Q000502:physiology*; D011374:Progesterone / Q000494:pharmacology*; D017693:Sodium Bicarbonate / Q000494:pharmacology
chemical_list: D003470:Culture Media; D006065:Gonadotropins, Pituitary; D011374:Progesterone; D017693:Sodium Bicarbonate