title: Candida albicans adherence to an acrylic resin modified by experimental photopolymerised coatings: an in vitro study.
abstract: OBJECTIVE
  This study evaluated whether photopolymerised coatings containing zwitterion or hydrophilic monomers would reduce the adhesion of Candida albicans to an acrylic resin.
  
  
  METHODS
  Disc-shaped samples (n = 468) were fabricated with rough or smooth surfaces. The samples did not receive any surface treatment (control) or were coated with one of the following experimental coatings (2-hydroxyethyl methacrylate - HE; 3-hydroxypropyl methacrylate - HP; and 2-trimethylammonium ethyl methacrylate chloride - T; and sulfobetaine methacrylate - S). The concentrations of the constituent monomers were 25, 30 or 35%. The water contact angles of the samples were measured, and half of the samples were exposed to saliva. The adherent yeast cells were counted after crystal violet staining.
  
  
  RESULTS
  For the smooth samples, the groups S35, HP35 and HE35 showed significantly lower number of adhered Candida than control, in the absence of saliva. There were no significant differences among the experimental and control groups for the rough samples, but the saliva decreased the cell numbers for groups S25, S30 and HP30. The photoelectron spectroscopy analysis confirmed the changes in the chemical compositions of the experimental samples.
  
  
  CONCLUSIONS
  The experimental photopolymerised coatings changed the chemical composition and decreased C. albicans adhesion in the groups S35, HP35 and HE35, suggesting that they should be further investigated.
authors: Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP - Univ Estadual Paulista, São Paulo, CEP, Brazil.
authors: Andrea Azevedo
authors: AA
authors: Lazarin
authors: Camila Andrade
authors: CA
authors: Zamperini
authors: Carlos Eduardo
authors: CE
authors: Vergani
authors: Amanda Fucci
authors: AF
authors: Wady
authors: Eunice Teresinha
authors: ET
authors: Giampaolo
authors: Ana Lucia
authors: AL
authors: Machado
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000328:Adult; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D002176:Candida albicans / Q000502:physiology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015169:Colony Count, Microbial; D004396:Coloring Agents; D003764:Dental Materials / Q000737:chemistry*; D044622:Dental Pellicle / Q000737:chemistry; D005260:Female; D005840:Gentian Violet; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055115:Light-Curing of Dental Adhesives; D008297:Male; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D011109:Polymethacrylic Acids / Q000737:chemistry; D013499:Surface Properties; D017155:Wettability
chemical_list: C120889:2-(trimethylammonio)ethyl methacrylate; D000180:Acrylic Resins; D020099:Coated Materials, Biocompatible; D004396:Coloring Agents; D003764:Dental Materials; D008689:Methacrylates; D011109:Polymethacrylic Acids; C521589:sulfobetaine methacrylate polymer; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate; D005840:Gentian Violet; C032802:hydroxypropyl methacrylate