title: A slow gradient approach for the purification of synthetic polypeptides by reversed phase high performance liquid chromatography.
abstract: Unquestionably, the purification of polypeptides by chromatographic methods is a considerable bottleneck in their preparation. Peptides synthesised by solid phase synthesis typically contain chromatographically similar impurities that complicate purification by reversed phase high performance liquid chromatography (HPLC) techniques. We report on the application of a slow gradient HPLC protocol that allows, in a single chromatographic step, the purification of hundreds of milligrammes of material. This technique was applied to an extensive collection of synthetic polypeptides some incorporating non-proteinogenic functionality. In all cases examined, the peptides were not only obtained in high purity peptides but were also recovered in multi-milligramme amounts.
authors: School of Chemical Sciences, The University of Auckland, 23 Symonds St, Auckland, 1142, New Zealand. paul.harris@auckland.ac.nz
authors: Paul W R
authors: PW
authors: Harris
authors: Dong Jun
authors: DJ
authors: Lee
authors: Margaret A
authors: MA
authors: Brimble
mesh_terms: D056148:Chromatography, Reverse-Phase / Q000379:methods*; D010455:Peptides / Q000302:isolation & purification*
chemical_list: D010455:Peptides