title: Determination of peimine and peiminine in Bulbus Fritillariae Thunbergii by capillary electrophoresis by indirect UV detection using N-(1-naphthyl)ethylenediamine dihydrochloride as probe.
abstract: A simple and inexpensive CE method was developed for the determination of peimine and peiminine. Because of the lack of an UV chromophore of peimine and peiminine, the detection method chosen was indirect UV detection, with N-(1-naphthyl)ethylenediamine dihydrochloride (NED) as the UV absorbing probe. It was thought that NED, a chromophoric ion, may form hydrogen bonding pairs with the analytes to cause significant changes in separation selectivity. Additionally, the hydrophobic interactions between analytes and the probe also play a crucial role in achieving a resolution between the two analytes. The analyses were carried out with a background electrolyte composed of 66% MeOH-ACN (1:1, v/v), 34% aqueous buffer containing 15 mM NaH₂PO₄, 2.5 mM NED, 4 mM H₃PO₄. MeOH-ACN mixtures used as organic modifiers can not only reduce the adsorption of NED to the capillary wall, but also decrease the baseline noise and drift. The method provided a linear response ranging from 5 to 200 μg/mL. The limits of detection (LODs) for peimine and peiminine were 3.9 and 4.1 μg/mL, respectively. The repeatabilities (n = 3) reached relative standard deviation values (RSDs) of 3.4 and 4.1% for the peak areas, 4.0 and 4.4% for the peak heights, and 0.29 and 0.30% for the migration time of peimine and peiminine, respectively. Regression equations revealed linear relationships (r = 0.9995-0.9996) between the peak area of each analyte and the concentration. The method developed was successfully applied to quantify peimine and peiminine in chloroform extracts of the ground Bulbus Fritillariae Thunbergii.
authors: School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China. lhliu02@126.com
authors: Lihong
authors: L
authors: Liu
authors: Wenwei
authors: W
authors: You
authors: Liujuan
authors: L
authors: Zheng
authors: Feiyu
authors: F
authors: Chen
authors: Zhimin
authors: Z
authors: Jia
mesh_terms: D002595:Cevanes / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005029:Ethylenediamines / Q000737:chemistry*; D031410:Fritillaria / Q000737:chemistry*; D057230:Limit of Detection; D018517:Plant Roots / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D002595:Cevanes; D004365:Drugs, Chinese Herbal; D005029:Ethylenediamines; C008588:N-(1-naphthyl)ethylenediamine; C014242:verticine; C047331:peiminine