title: FAST-SeqS: a simple and efficient method for the detection of aneuploidy by massively parallel sequencing.
abstract: Massively parallel sequencing of cell-free, maternal plasma DNA was recently demonstrated to be a safe and effective screening method for fetal chromosomal aneuploidies. Here, we report an improved sequencing method achieving significantly increased throughput and decreased cost by replacing laborious sequencing library preparation steps with PCR employing a single primer pair designed to amplify a discrete subset of repeated regions. Using this approach, samples containing as little as 4% trisomy 21 DNA could be readily distinguished from euploid samples.
authors: The Howard Hughes Medical Institute, Johns Hopkins Kimmel Cancer Center, Baltimore, Maryland, United States of America. kjeskine@loyno.edu
authors: Isaac
authors: I
authors: Kinde
authors: Nickolas
authors: N
authors: Papadopoulos
authors: Kenneth W
authors: KW
authors: Kinzler
authors: Bert
authors: B
authors: Vogelstein
mesh_terms: D000328:Adult; D000782:Aneuploidy; D002869:Chromosome Aberrations; D025063:Chromosome Disorders / Q000175:diagnosis* / Q000235:genetics*; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011248:Pregnancy Complications / Q000175:diagnosis / Q000235:genetics; D011296:Prenatal Diagnosis / Q000379:methods*; D017422:Sequence Analysis, DNA; D014314:Trisomy
chemical_list: D017931:DNA Primers; D004247:DNA