title: Quantitative genomic and antigenomic enterovirus RNA detection in explanted heart tissue samples from patients with end-stage idiopathic dilated cardiomyopathy.
abstract: Standardized one-step real-time RT-PCR assay detected enterovirus RNA in cardiac biopsy samples from 4 of 20 patients suffering from idiopathic dilated cardiomyopathy (IDCM). The median viral load was 287 copies per microgram of total extracted nucleic acids, with positive- to negative-strand RNA ratios ranging from 2 to 20. These results demonstrate enterovirus persistence in the heart of IDCM patients, characterized by low viral loads and low positive- to negative-RNA ratios.
authors: Clinical and Molecular Virology Unit, University Hospital, Reims, France. nleveque@chu-reims.fr
authors: Nicolas
authors: N
authors: Lévêque
authors: Fanny
authors: F
authors: Renois
authors: Déborah
authors: D
authors: Talmud
authors: Yohan
authors: Y
authors: Nguyen
authors: François
authors: F
authors: Lesaffre
authors: Camille
authors: C
authors: Boulagnon
authors: Patrick
authors: P
authors: Bruneval
authors: Paul
authors: P
authors: Fornes
authors: Laurent
authors: L
authors: Andréoletti
mesh_terms: D000328:Adult; D000818:Animals; D001706:Biopsy; D002311:Cardiomyopathy, Dilated / Q000473:pathology / Q000821:virology*; D004770:Enterovirus / Q000235:genetics / Q000302:isolation & purification*; D006321:Heart / Q000821:virology*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019562:Viral Load
chemical_list: D012367:RNA, Viral