title: On a Class of Admissible Constitutive Behaviors in Free-Floating Engineered Tissues.
abstract: A commonly used assay for studying cell - matrix interactions is the free-floating fibroblast populated collagen lattice, which was introduced in 1979. Briefly, fibroblasts are seeded within an initially thin, amorphous, untethered, circular gel consisting of reconstituted fibrillar collagen. Although the gel remains traction free and circular, the cells typically contract the gel to less than 50% of its original diameter within hours to days. Cellular mechanotransduction mechanisms are fundamental to this contraction, but there has not been a careful study of the associated mechanics. In this paper, we model the initial contraction of a circular gel by assuming a homogeneous, axisymmetric finite deformation while allowing possible radial variations in material properties, including material symmetry. We show that trivial solutions alone (i.e., no deformation, no contraction) are admitted by equilibrium and boundary conditions unless radial variations exist in the material behavior, including cell contraction. Although more complete data are needed to model better this initial-boundary value problem, the present results are consistent with both the salient features of the gel assay and recent observations reported in the literature that cells often introduce regional variations in tissue properties in vivo in an attempt to achieve, maintain, or restore mechanical homeostasis.
authors: Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
authors: D D
authors: DD
authors: Simon
authors: J D
authors: JD
authors: Humphrey