title: Survivin promoter -31G/C polymorphism in oral cancer cell lines.
abstract: Survivin (SVV) is a protein that belongs to the inhibitor of apoptosis proteins (IAP) family and is involved in the G2/M phase progression of the cell cycle as a spindle‑associated molecule. The biological features of this protein are well documented and its activity appears to be involved in mitochondria-dependent and -independent antiapoptotic pathways. Overexpression of SVV at the transcriptional and translational level has been associated with cancer, a multifactorial disorder in which the occurrence of a -31G to C polymorphism in the promoter region may significantly contribute to the development of this pathology. To verify this hypothesis, the occurrence of a single nucleotide polymorphism (SNP) in cis-acting cell cycle-dependent elements (CDEs) and in cell cycle homology regions (CHRs) of the survivin TATA-less promoter was investigated. A total of 23 oral squamous cell carcinoma (OSCC) cell lines and normal epithelium-derived normal human epidermal keratinocyte (NHEK) cell lines were analyzed by RFLP and direct DNA sequencing of their promoter region. Furthermore, survivin expression at the transcriptional and translational levels was evaluated in these cells by RT-PCR and Western blotting, respectively. The findings indicate that the presence of a G or C allele is not directly correlated to survivin expression, at the mRNA or at the protein level, at least in the OSCC lines analyzed in this study.
authors.affiliation: Department of Experimental Medicine, Medical School, Second University of Naples, I-80138 Naples, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: de Maria
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Lo Muzio
authors.forename: Alessandra
authors.initials: A
authors.lastname: Braca
authors.forename: Paolo
authors.initials: P
authors.lastname: Rega
authors.forename: Amalia
authors.initials: A
authors.lastname: Cassano
authors.forename: Angela
authors.initials: A
authors.lastname: Vinella
authors.forename: Ruggiero
authors.initials: R
authors.lastname: Fumarulo
authors.forename: Rosario
authors.initials: R
authors.lastname: Serpico
authors.forename: Ernesto
authors.initials: E
authors.lastname: Farina
authors.forename: Vittoria
authors.initials: V
authors.lastname: Metafora
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pannone
authors.forename: Gian Pietro
authors.initials: GP
authors.lastname: Ravagnan
authors.forename: Salvatore
authors.initials: S
authors.lastname: Metafora
authors.forename: Corrado
authors.initials: C
authors.lastname: Rubini
authors.forename: Maria
authors.initials: M
authors.lastname: Carteni
authors.forename: Maria Addolorata
authors.initials: MA
authors.lastname: Mariggiò