title: [Development of a loop-mediated isothermal amplification assay for detecting Vibrio parahaemolyticus].
abstract: OBJECTIVE
  This study aimed to establish a loop-mediated isothermal amplification (LAMP) assay for detection of Vibrio parahaemolyticus (V. parahaemolyticus).
  
  
  METHODS
  The specificity of this assay was evaluated by using a panel of 33 strains of V. parahaemolyticus and 22 strains of other species bacteria. The sensitivity was determined by using serial dilutions of V. parahaemolyticus (ATCC 17802) chromosomal DNA (5×10(0) - 5×10(5) copies/µl). The samples were also tested by using qualification PCR assay and Taqman real-time PCR assay in parallel for comparison with LAMP.
  
  
  RESULTS
  Both sensitivity and specificity of LAMP assay, PCR assay and Taqman real-time PCR assay were 100% (22/22, 33/33, respectively). The detection limits of above three methods assay were 5×10(1) copies/µl, 5×10(3) copies/µl and 5×10(2) copies/µl, respectively. The reaction period of time needed of the above three assays was 22 min, 3 h, 50 min, respectively.
  
  
  CONCLUSIONS
  Compared to qualification PCR assay and Taqman real-time PCR assay, the established LAMP assay was better in low detection limit and less reaction time, which made it an ideal method for quick detection of V. parahaemolyticus.
authors.affiliation: Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.forename: Shu-jing
authors.initials: SJ
authors.lastname: Wang
authors.forename: Sha
authors.initials: S
authors.lastname: Liu
authors.forename: Biao
authors.initials: B
authors.lastname: Kan
authors.forename: Bo
authors.initials: B
authors.lastname: Pang
mesh_terms: D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014736:Vibrio parahaemolyticus / Q000235:genetics / Q000302:isolation & purification*