title: In vitro evaluation of antibacterial activities and anti-inflammatory effects of Bifidobacterium spp. addressing acne vulgaris.
abstract: The objective of this study was to evaluate the antibacterial and anti-inflammatory effect of Bifidobacterium spp. In the first part of the study, the antibacterial activities of live and sonicated cells, from a total of 23 Bifidobacterium species, on the growth of 5 different strain of Staphylococcus aureus. Six strains, of sonicated Bifidobacterium, exhibited antibacterial activity against staphylococci samples, and seven Bifidobacterium strains exhibited antibacterial activity on the growth of S. aureus S.P.-N2. In the second part of the study, we tested the antimicrobial activity, of Bifidobacterium against Propionibacterium acne KCTC3320, using the co-culture method. The loss of P. acnes viability, caused by B. adolescentis SPM0308 and B. longum SPM1207, was 84% and 75%, respectively (*p < 0.05). In the third part of the study, the anti-inflammatory activity of B. adolescentis SPM0308 and B. longum SPM1207 were assessed; nitric oxide (NO), and tumor necrosis factor-α (TNF-α), production were tested using the murine macrophage RAW 264.7 cell line. Treatment of RAW 264.7 cells, with Bifidobacterium, decreased production of NO and TNF-α rather than LPS (100 ng/mL) treatment. The results suggest that B. adolescentis SPM0308 could be used as an effective control for P. acnes KCTC3320, and S. aureus, and reduce the risk of acne vulgaris development. We suggest that B. adolescentis SPM0308 may be a useful probiotic microorganism, for prevention of acne vulgaris, without adverse effects.
authors.affiliation: College of Pharmacy, Sahmyook University, Seoul 139-742, Korea.
authors.forename: Do-Kyung
authors.initials: DK
authors.lastname: Lee
authors.forename: Mi-Jin
authors.initials: MJ
authors.lastname: Kim
authors.forename: Ji-Wook
authors.initials: JW
authors.lastname: Ham
authors.forename: Hyang-Mi
authors.initials: HM
authors.lastname: An
authors.forename: Min-Kyung
authors.initials: MK
authors.lastname: Cha
authors.forename: Si-Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Cheol-In
authors.initials: CI
authors.lastname: Park
authors.forename: Seon-Hee
authors.initials: SH
authors.lastname: Shin
authors.forename: Kang-Oh
authors.initials: KO
authors.lastname: Lee
authors.forename: Kyung-Jae
authors.initials: KJ
authors.lastname: Kim
authors.forename: Nam-Joo
authors.initials: NJ
authors.lastname: Ha
mesh_terms: D000152:Acne Vulgaris / Q000276:immunology / Q000382:microbiology / Q000628:therapy*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D001644:Bifidobacterium / Q000502:physiology*; D002460:Cell Line; D052978:Disk Diffusion Antimicrobial Tests; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D009569:Nitric Oxide / Q000378:metabolism; D011425:Propionibacterium acnes / Q000187:drug effects / Q000254:growth & development*; D013010:Sonication; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide