title: Acute diarrhea in West African children: diverse enteric viruses and a novel parvovirus genus.
abstract: Parvoviruses cause a variety of mild to severe symptoms or asymptomatic infections in humans and animals. During a viral metagenomic analysis of feces from children with acute diarrhea in Burkina Faso, we identified in decreasing prevalence nucleic acids from anelloviruses, dependoviruses, sapoviruses, enteroviruses, bocaviruses, noroviruses, adenoviruses, parechoviruses, rotaviruses, cosavirus, astroviruses, and hepatitis B virus. Sequences from a highly divergent parvovirus, provisionally called bufavirus, were also detected whose NS1 and VP1 proteins showed <39% and <31% identities to those of previously known parvoviruses. Four percent of the fecal samples were PCR positive for this new parvovirus, including a related bufavirus species showing only 72% identity in VP1. The high degree of genetic divergence of these related genomes from those of other parvoviruses indicates the presence of a proposed new Parvoviridae genus containing at least two species. Studies of the tropism and pathogenicity of these novel parvoviruses will be facilitated by the availability of their genome sequences.
authors.affiliation: Blood Systems Research Institute, San Francisco, California, USA.
authors.forename: Tung G
authors.initials: TG
authors.lastname: Phan
authors.forename: Nguyen P
authors.initials: NP
authors.lastname: Vo
authors.forename: Isidore J O
authors.initials: IJ
authors.lastname: Bonkoungou
authors.forename: Amit
authors.initials: A
authors.lastname: Kapoor
authors.forename: Nicolas
authors.initials: N
authors.lastname: Barro
authors.forename: Miguel
authors.initials: M
authors.lastname: O'Ryan
authors.forename: Beatrix
authors.initials: B
authors.lastname: Kapusinszky
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
mesh_terms: D001483:Base Sequence; D002050:Burkina Faso; D036022:Capsid Proteins / Q000235:genetics* / Q000302:isolation & purification; D002675:Child, Preschool; D003967:Diarrhea / Q000821:virology*; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D010322:Parvoviridae Infections / Q000821:virology*; D017992:Parvovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D036022:Capsid Proteins; C106202:NS1 protein, parvovirus; D017361:Viral Nonstructural Proteins; C095547:capsid protein VP1, parvovirus B19