title: [UPLC/Q-TOF-MS-based chemical profiling approach to evaluate the chemical constitution of Radix Aconiti Lateralis Preparata in the process of decoction].
abstract: OBJECTIVE
  An ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/Q-TOF-MS)-based chemical analytic technology was used to evaluate the chemical constitution of Radix Aconiti Lateralis Preparata in the process of decocting, so as to provide a scientific basis for processing Radix Aconiti Lateralis Preparata.
  
  
  METHODS
  Radix Aconiti Lateralis Preparata samples with different decocting time were detected using UPLC/Q-TOF-MS to create a fingerprint spectrum. The data of tR-m/z pairs, ion intensities and sample codes were processed by partial least squared-discriminant analysis (PLS-DA) to holistically compare the difference among these samples.
  
  
  RESULTS
  The contents of diester-diterpenoid alkaloid such as aconitine, mesaconitine and hypaconitine were higher between 2 to 10 min, while the contents of the lower toxic monoester-diterpenoid alkaloids such as benzoylaconine, benzoylmesaconine and benzoylhypaconine increased during the first 60 min, then their contents stabilized.
  
  
  CONCLUSIONS
  Selecting the correct processing time for Radix Aconiti Lateralis Preparata according to clinical indications is essential for attenuating toxicity and increasing efficiency. Within 30 min is the best time for diester-diterpenoid alkaloids as the main pharmacodynamic material, and within 60 min is the best time for monoester-diterpenoid alkaloids as the pharmacodynamic material of Radix Aconiti Lateralis Preparata.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Si-Si
authors.initials: SS
authors.lastname: Zhou
authors.forename: Zeng-Chun
authors.initials: ZC
authors.lastname: Ma
authors.forename: Qian-de
authors.initials: QD
authors.lastname: Liang
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Wang
authors.forename: Hong-Ling
authors.initials: HL
authors.lastname: Tan
authors.forename: Cheng-Rong
authors.initials: CR
authors.lastname: Xiao
authors.forename: Bo-Li
authors.initials: BL
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives / Q000032:analysis; D031841:Aconitum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: C019470:mesaconitine; D000157:Aconitine