title: Coiled-coil networking shapes cell molecular machinery.
abstract: The highly abundant α-helical coiled-coil motif not only mediates crucial protein-protein interactions in the cell but is also an attractive scaffold in synthetic biology and material science and a potential target for disease intervention. Therefore a systematic understanding of the coiled-coil interactions (CCIs) at the organismal level would help unravel the full spectrum of the biological function of this interaction motif and facilitate its application in therapeutics. We report the first identified genome-wide CCI network in Saccharomyces cerevisiae, which consists of 3495 pair-wise interactions among 598 predicted coiled-coil regions. Computational analysis revealed that the CCI network is specifically and functionally organized and extensively involved in the organization of cell machinery. We further show that CCIs play a critical role in the assembly of the kinetochore, and disruption of the CCI network leads to defects in kinetochore assembly and cell division. The CCI network identified in this study is a valuable resource for systematic characterization of coiled coils in the shaping and regulation of a host of cellular machineries and provides a basis for the utilization of coiled coils as domain-based probes for network perturbation and pharmacological applications.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xinlei
authors.initials: X
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Haolong
authors.initials: H
authors.lastname: Huang
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Dong
authors.forename: Jinan
authors.initials: J
authors.lastname: Chen
authors.forename: Jiuhong
authors.initials: J
authors.lastname: Dong
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Haiying
authors.initials: H
authors.lastname: Hang
authors.forename: Taijiao
authors.initials: T
authors.lastname: Jiang
mesh_terms: D016681:Genome, Fungal; D018386:Kinetochores / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008954:Models, Biological; D058977:Molecular Sequence Annotation; D054730:Protein Interaction Domains and Motifs; D060066:Protein Interaction Maps; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D029701:Saccharomyces cerevisiae Proteins