title: Daily quality control in CD3+ and CD4+ T cell estimation by the FACSCount system at a tertiary care center in south India.
abstract: CD4(+) T cell count estimations are subject to high variations; hence, in this study, the previous day's tested samples were included routinely as the internal quality controls. The percentages of variation of the 2-day values were analyzed for 280 observations and the mean variation for CD4(+) and CD3(+) T cell counts ranged from 5.21% to 9.66%. This method is a good internal quality control (IQC) procedure for the estimation of CD3(+) and CD4(+) T cell counts in resource-poor settings.
authors.affiliation: Department of Clinical Virology, Christian Medical College, Vellore, India.
authors.forename: Veena V
authors.initials: VV
authors.lastname: Ramalingam
authors.forename: Monika
authors.initials: M
authors.lastname: Mani
authors.forename: Vijayanand C
authors.initials: VC
authors.lastname: Sundaresan
authors.forename: Ramesh J
authors.initials: RJ
authors.lastname: Karunaiya
authors.forename: Jaiprasath
authors.initials: J
authors.lastname: Sachithanandham
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kannangai
mesh_terms: D017252:CD3 Complex / Q000032:analysis*; D015704:CD4 Antigens / Q000032:analysis*; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology; D006801:Humans; D007194:India; D011786:Quality Control; D012044:Regression Analysis; D062606:Tertiary Care Centers
chemical_list: D017252:CD3 Complex; D015704:CD4 Antigens