title: Dual lineage-specific expression of Sox17 during mouse embryogenesis.
abstract: Sox17 is essential for both endoderm development and fetal hematopoietic stem cell (HSC) maintenance. While endoderm-derived organs are well known to originate from Sox17-expressing cells, it is less certain whether fetal HSCs also originate from Sox17-expressing cells. By generating a Sox17(GFPCre) allele and using it to assess the fate of Sox17-expressing cells during embryogenesis, we confirmed that both endodermal and a part of definitive hematopoietic cells are derived from Sox17-positive cells. Prior to E9.5, the expression of Sox17 is restricted to the endoderm lineage. However, at E9.5 Sox17 is expressed in the endothelial cells (ECs) at the para-aortic splanchnopleural region that contribute to the formation of HSCs at a later stage. The identification of two distinct progenitor cell populations that express Sox17 at E9.5 was confirmed using fluorescence-activated cell sorting together with RNA-Seq to determine the gene expression profiles of the two cell populations. Interestingly, this analysis revealed differences in the RNA processing of the Sox17 mRNA during embryogenesis. Taken together, these results indicate that Sox17 is expressed in progenitor cells derived from two different germ layers, further demonstrating the complex expression pattern of this gene and suggesting caution when using Sox17 as a lineage-specific marker.
authors.affiliation: Center for Stem Cell Biology and Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee 37232-0494, USA.
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Choi
authors.forename: Marine R C
authors.initials: MR
authors.lastname: Kraus
authors.forename: Laurence A
authors.initials: LA
authors.lastname: Lemaire
authors.forename: Momoko
authors.initials: M
authors.lastname: Yoshimoto
authors.forename: Sasidhar
authors.initials: S
authors.lastname: Vemula
authors.forename: Leah A
authors.initials: LA
authors.lastname: Potter
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Manduchi
authors.forename: Christian J
authors.initials: CJ
authors.lastname: Stoeckert
authors.forename: Anne
authors.initials: A
authors.lastname: Grapin-Botton
authors.forename: Mark A
authors.initials: MA
authors.lastname: Magnuson
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D004622:Embryo, Mammalian; D047108:Embryonic Development; D004707:Endoderm / Q000166:cytology / Q000378:metabolism; D053686:Fetal Stem Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics; D024002:HMGB Proteins / Q000235:genetics* / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000096:biosynthesis; D055760:SOXF Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D024002:HMGB Proteins; D012333:RNA, Messenger; D055760:SOXF Transcription Factors; C511606:Sox17 protein, mouse; D049452:Green Fluorescent Proteins