title: Identification of seven water-soluble non-storage proteins from pomegranate (Punica granatum Linn.) seeds.
abstract: As pomegranate (Punica granatum Linn.) processing is fast growing, the usage of pomegranate processing wastes containing seeds has been receiving great attention. The protein component accounts for 100-130 g/kg of the seeds in weight. However, so far, there is no information on the composition and function of the pomegranate seed proteins. In this study, a global view of water-soluble non-storage proteins isolated from mature pomegranate seeds were studied using two-dimensional polyacrylamide gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry. With the two-dimensional polyacrylamide gel electrophoresis approach, over 120 protein spots were resolved, of which 7 abundant protein spots showing low molecular mass were identified. These identified proteins may be linked to seed development and metabolism, but more importantly, the occurrence of these proteins provides the possibility of conversion the pomegranate processing wastes into useful products or raw material for food industry.
authors.affiliation: CAU & ACC Joint-Laboratory of Space Food, College of Food Science and Nutritional Engineering, China Agricultural University, Key Laboratory of Functional Dairy, Ministry of Education, Beijing 100083, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Yang
authors.forename: Meiliang
authors.initials: M
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.forename: Chenyan
authors.initials: C
authors.lastname: Lv
authors.forename: Jianjun
authors.initials: J
authors.lastname: Deng
authors.forename: Guanghua
authors.initials: G
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019587:Dietary Supplements / Q000191:economics; D015180:Electrophoresis, Gel, Two-Dimensional; D005525:Food-Processing Industry / Q000191:economics; D007220:Industrial Waste / Q000032:analysis / Q000191:economics; D029561:Lythraceae / Q000201:enzymology / Q000378:metabolism*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010449:Peptide Mapping; D010940:Plant Proteins / Q000737:chemistry / Q000191:economics / Q000302:isolation & purification / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology / Q000378:metabolism*; D012995:Solubility; D053719:Tandem Mass Spectrometry
chemical_list: D007220:Industrial Waste; D010446:Peptide Fragments; D010940:Plant Proteins