title: Sequencing the miRNAs in maternal plasma from women before and after parturition.
abstract: The goal of the study was to analyze the microRNAs (miRNA) expression that differentiates in maternal plasma of women before and after parturition. Here, we used high throughput sequencer to analyze the expression change of all microRNAs in plasma from pregnant women. Six families of microRNA were also surveyed using real-time quantitative PCR. Sequencing result showed that the circulating microRNA expression in plasma from pregnant women down regulated remarkably after parturition. The quantitative PCR results showed that the differential expression of most miRNAs in plasma between before and after parturition was consistent with the sequencing result. It is also showed that not only notable differential miRNA expression between the plasma from the same woman collected before and after parturition, but also between normal plasma and preeclamptic plasma, which indicate that miRNAs could be potential biomarker for prenatal diagnosis and prognosis. The study suggested circulating miRNAs in plasma of pregnant women could be detected more comprehensive by the next generation sequencing technology. This research also suggested the differential miRNA expression could be related to the existence and clearance of circulating DNA/RNA, which paved a new way for studying the origin and path of circulating nucleic acid.
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
authors.forename: Min
authors.initials: M
authors.lastname: Pan
authors.forename: Qinyu
authors.initials: Q
authors.lastname: Ge
authors.forename: Hailing
authors.initials: H
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Lu
authors.forename: Dingdong
authors.initials: D
authors.lastname: Zhang
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D005260:Female; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D035683:MicroRNAs / Q000097:blood* / Q000737:chemistry / Q000235:genetics*; D036801:Parturition / Q000097:blood* / Q000235:genetics*; D011225:Pre-Eclampsia / Q000097:blood / Q000235:genetics; D011247:Pregnancy / Q000097:blood* / Q000235:genetics*; D011296:Prenatal Diagnosis / Q000379:methods; D055815:Young Adult
chemical_list: D015415:Biomarkers; D035683:MicroRNAs