title: Carbon nanocapsules as an effective sensing platform for fluorescence-enhanced nucleic acid detection.
abstract: In this communication, we demonstrate the proof of concept that carbon nanocapsules (CNCs) can be used as an effective fluorescent sensing platform for nucleic acid detection with selectivity down to single-base mismatch. The detection is accomplished by two steps: (1) CNC adsorbs and quenches the fluorescence of the dye-labeled single-stranded DNA (ssDNA) probe; (2) in the presence of the target, a hybridization event occurs, which produces a double-stranded DNA (dsDNA) that detaches from the CNC surface, leading to recovery of the dye fluorescence.
authors.affiliation: Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Industry, China West Normal University, Nanchong 637002, Sichuan, China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Chang
authors.forename: Jianming
authors.initials: J
authors.lastname: Hu
authors.forename: Wenbo
authors.initials: W
authors.lastname: Lu
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Qin
authors.forename: Abdullah M
authors.initials: AM
authors.lastname: Asiri
authors.forename: Abdulrahman O
authors.initials: AO
authors.lastname: Al-Youbi
authors.forename: Xuping
authors.initials: X
authors.lastname: Sun
mesh_terms: D020137:Base Pair Mismatch; D002244:Carbon / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D053769:Nanocapsules / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods; D009696:Nucleic Acids / Q000032:analysis* / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D053769:Nanocapsules; D009696:Nucleic Acids; D002244:Carbon; D004247:DNA