title: miRNA as activity markers in Parvo B19 associated heart disease.
abstract: Parvovirus B19 is a frequent virus detected in endomyocardial biopsies of patients with clinically suspected myocarditis or dilated cardiomyopathy (DCM). Viruses often cause a more symptomatic disease with increased tissue injury if they become reactivated. A disease-specific differential expression of microRNAs (miRNAs) has been described in the regulation of replicating viruses. Analyzing patients with latent and reactivated B19V infection, we found 29 differentially regulated miRNAs and, in order to test whether predicted genes are differentially expressed, selected mRNAs were tested by TaqMan-QPCR.
authors.affiliation: Department of Cardiology and Pneumology, Campus Benjamin Franklin, Charité - Universitätsmedizin Berlin, Hindenburgdamm 30, 12200, Berlin, Germany. uwe.kuehl@charite.de
authors.forename: U
authors.initials: U
authors.lastname: Kühl
authors.forename: M
authors.initials: M
authors.lastname: Rohde
authors.forename: D
authors.initials: D
authors.lastname: Lassner
authors.forename: U M
authors.initials: UM
authors.lastname: Gross
authors.forename: F
authors.initials: F
authors.lastname: Escher
authors.forename: H-P
authors.initials: HP
authors.lastname: Schultheiss
mesh_terms: D009202:Cardiomyopathies / Q000175:diagnosis / Q000235:genetics* / Q000821:virology*; D005260:Female; D005819:Genetic Markers / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000235:genetics* / Q000821:virology*; D016732:Parvovirus B19, Human / Q000302:isolation & purification*
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs