title: Comparison of the fecal microbiota of healthy horses and horses with colitis by high throughput sequencing of the V3-V5 region of the 16S rRNA gene.
abstract: The intestinal tract houses one of the richest and most complex microbial populations on the planet, and plays a critical role in health and a wide range of diseases. Limited studies using new sequencing technologies in horses are available. The objective of this study was to characterize the fecal microbiome of healthy horses and to compare the fecal microbiome of healthy horses to that of horses with undifferentiated colitis. A total of 195,748 sequences obtained from 6 healthy horses and 10 horses affected by undifferentiated colitis were analyzed. Firmicutes predominated (68%) among healthy horses followed by Bacteroidetes (14%) and Proteobacteria (10%). In contrast, Bacteroidetes (40%) was the most abundant phylum among horses with colitis, followed by Firmicutes (30%) and Proteobacteria (18%). Healthy horses had a significantly higher relative abundance of Actinobacteria and Spirochaetes while horses with colitis had significantly more Fusobacteria. Members of the Clostridia class were more abundant in healthy horses. Members of the Lachnospiraceae family were the most frequently shared among healthy individuals. The species richness reported here indicates the complexity of the equine intestinal microbiome. The predominance of Clostridia demonstrates the importance of this group of bacteria in healthy horses. The marked differences in the microbiome between healthy horses and horses with colitis indicate that colitis may be a disease of gut dysbiosis, rather than one that occurs simply through overgrowth of an individual pathogen.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada. costamc@gmail.com
authors.forename: Marcio C
authors.initials: MC
authors.lastname: Costa
authors.forename: Luis G
authors.initials: LG
authors.lastname: Arroyo
authors.forename: Emma
authors.initials: E
authors.lastname: Allen-Vercoe
authors.forename: Henry R
authors.initials: HR
authors.lastname: Stämpfli
authors.forename: Peter T
authors.initials: PT
authors.lastname: Kim
authors.forename: Amy
authors.initials: A
authors.lastname: Sturgeon
authors.forename: J Scott
authors.initials: JS
authors.lastname: Weese
mesh_terms: D000818:Animals; D003092:Colitis / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D059014:High-Throughput Nucleotide Sequencing; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D054892:Metagenome; D008957:Models, Genetic; D010802:Phylogeny; D025341:Principal Component Analysis; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S