title: Purification and differential biological effects of ginger-derived substances on normal and tumor cell lines.
abstract: This study describes an optimization of [6]-, [8]- and [10]-gingerol isolation and purification in semi-preparative HPLC scale and their anti-proliferative activity. The gingerols purification was carried out in HPLC system using a Luna-C₁₈ and the best mobile phase evaluated was MeOH/H₂O (75:25, v/v). This new methodology for the gingerols isolation was very effective, since considerable amounts (in the range of milligrams) with a good purity degree (∼98%) were achieved in 30 min of chromatographic run. [6]-, [8]- and [10]-Gingerol purified by this methodology inhibited the proliferation of MDA-MB-231 tumor cell line with IC₅₀ of 666.2±134.6 μM, 135.6±22.6 μM and 12.1±0.3 μM, respectively. These substances also inhibited human fibroblasts (HF) cell proliferation, however in concentrations starting from 500 μM. In conclusion, our results demonstrate an optimization of gingerols isolation and their specific anti-proliferative activities against tumor cells, suggesting their use as important models for drug design in an attempt to develop new compounds with fewer side effects when compared to conventional chemotherapy.
authors.affiliation: Departamento de Química, Rodovia Washington Luís, Km 235, CEP 13565-905, São Carlos, SP, Brazil.
authors.forename: James
authors.initials: J
authors.lastname: Almada da Silva
authors.forename: Amanda Blanque
authors.initials: AB
authors.lastname: Becceneri
authors.forename: Hêmily
authors.initials: H
authors.lastname: Sanches Mutti
authors.forename: Ana Carolina Baptista
authors.initials: AC
authors.lastname: Moreno Martin
authors.forename: Maria Fátima das Graças
authors.initials: MF
authors.lastname: Fernandes da Silva
authors.forename: João Batista
authors.initials: JB
authors.lastname: Fernandes
authors.forename: Paulo Cezar
authors.initials: PC
authors.lastname: Vieira
authors.forename: Márcia Regina
authors.initials: MR
authors.lastname: Cominetti
mesh_terms: D000704:Analysis of Variance; D002396:Catechols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005233:Fatty Alcohols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020939:Zingiber officinale / Q000737:chemistry*; D006801:Humans
chemical_list: D002396:Catechols; D005233:Fatty Alcohols; C007845:gingerol