title: Isolation and characterization of a sex-specific lectin in a marine red alga, Aglaothamnion oosumiense Itono.
abstract: In red algae, spermatial binding to female trichogynes is mediated by a lectin-carbohydrate complementary system. Aglaothamnion oosumiense is a microscopic filamentous red alga. The gamete recognition and binding occur at the surface of the hairlike trichogyne on the female carpogonium. Male spermatia are nonmotile. Previous studies suggested the presence of a lectin responsible for gamete recognition on the surface of female trychogynes. A novel N-acetyl-D-galactosamine-specific protein was isolated from female plants of A. oosumiense by affinity chromatography and named AOL1. The lectin was monomeric and did not agglutinate horse blood or human erythrocytes. The N-terminal amino acid sequence of the protein was analyzed, and degenerate primers were designed. A full-length cDNA encoding the lectin was obtained using rapid amplification of cDNA ends-PCR (RACE-PCR). The cDNA was 1,095 bp in length and coded for a protein of 259 amino acids with a deduced molecular mass of 21.4 kDa, which agreed well with the protein data. PCR analysis using genomic DNA showed that both male and female plants have this gene. However, Northern blotting and two-dimensional electrophoresis showed that this protein was expressed 12 to 15 times more in female plants. The lectin inhibited spermatial binding to the trichogynes when preincubated with spermatia, suggesting its involvement in gamete binding.
authors.affiliation: Department of Biology, Kongju National University, Kongju, South Korea.
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Han
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Klochkova
authors.forename: Jun Bo
authors.initials: JB
authors.lastname: Shim
authors.forename: Kangsup
authors.initials: K
authors.lastname: Yoon
authors.forename: Gwang Hoon
authors.initials: GH
authors.lastname: Kim
mesh_terms: D000372:Agglutination Tests; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002846:Chromatography, Affinity; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D004912:Erythrocytes / Q000187:drug effects; D020869:Gene Expression Profiling; D006736:Horses; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D000461:Rhodophyta / Q000737:chemistry* / Q000235:genetics; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein
chemical_list: D018076:DNA, Complementary; D037102:Lectins