title: [Investigation on Ehrlichia chaffeensis and Anaplasma phagocytophilum infection among farmers and domestic animals in rural areas of Beijing, China].
abstract: OBJECTIVE
  To investigate the status of Ehrlichia (E.) chaffeensis and Anaplasma (A.) phagocytophilum infection among farming populations and domestic animals in the rural area of Beijing, China.
  
  
  METHODS
  Blood samples from 562 farmers and 163 blood samples including 90 goats, 71 ox and 2 dogs, were collected. Specificity of IgG antibodies against E. chaffeensis and A. phagocytophilum were tested by micro-indirect immunofluorescent assay (mIFA). 16S rRNA genes of A. phagocytophilum were amplified from the domestic animal blood samples and products were sequenced and analyzed by nested PCR.
  
  
  RESULTS
  The positive rates of E. chaffeensis and A. phagocytophilum antibody were 16.5% and 14.0% respectively for farmers. The total positive rates of A. phagocytophilum were 2.3% and 0 for both goats and oxen respectively. No antibody was found for the 2 tested dogs. The PCR positive rates were 48.9% and 23.9% for goats and oxen respectively. Three dominant varieties of A. phagocytophilum were demonstrated in goats and oxen.
  
  
  CONCLUSIONS
  The prevalence rates of E. chaffeensis and A. phagocytophilum were identified in the rural areas of Beijing.
authors.affiliation: Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Xiu-chun
authors.initials: XC
authors.lastname: Zhang
authors.forename: Li-xia
authors.initials: LX
authors.lastname: Zhang
authors.forename: Wei-hong
authors.initials: WH
authors.lastname: Li
authors.forename: Shi-wen
authors.initials: SW
authors.lastname: Wang
authors.forename: Yu-lan
authors.initials: YL
authors.lastname: Sun
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Wang
authors.forename: Zeng-zhi
authors.initials: ZZ
authors.lastname: Guan
authors.forename: Xiu-jun
authors.initials: XJ
authors.lastname: Liu
authors.forename: Yu-song
authors.initials: YS
authors.lastname: Yang
authors.forename: Shu-guang
authors.initials: SG
authors.lastname: Zhang
authors.forename: Hui-lan
authors.initials: HL
authors.lastname: Yu
authors.forename: Li-juan
authors.initials: LJ
authors.lastname: Zhang
mesh_terms: D000328:Adult; D041081:Anaplasma phagocytophilum / Q000302:isolation & purification*; D000818:Animals; D000829:Animals, Domestic / Q000382:microbiology; D002417:Cattle; D002681:China / Q000453:epidemiology; D004285:Dogs; D018469:Ehrlichia chaffeensis / Q000302:isolation & purification*; D016873:Ehrlichiosis / Q000453:epidemiology*; D005260:Female; D006041:Goats; D006801:Humans; D008297:Male; D008875:Middle Aged