title: Yeast one-hybrid assays: a historical and technical perspective.
abstract: Since its development about two decades ago, the yeast one-hybrid (Y1H) assay has become an important technique for detecting physical interactions between sequence-specific regulatory transcription factor proteins (TFs) and their DNA target sites. Multiple versions of the Y1H methodology have been developed, each with technical differences and unique advantages. We will discuss several of these technical variations in detail, and also provide some ideas for how Y1H assays can be further improved.
authors.affiliation: Program in Systems Biology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA. john.reece-hoyes@umassmed.edu
authors.forename: John S
authors.initials: JS
authors.lastname: Reece-Hoyes
authors.forename: A J
authors.initials: AJ
authors.lastname: Marian Walhout
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D017930:Genes, Reporter; D049673:History, 20th Century; D006801:Humans; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000378:metabolism* / Q000502:physiology; D015533:Transcriptional Activation; D020798:Two-Hybrid System Techniques / Q000266:history
chemical_list: D014157:Transcription Factors