title: [Raman spectroscopic study on effect of danshen injection on human erythrocyte membranes and its mechanism].
abstract: OBJECTIVE
  Danshen, as a traditional Chinese medicine for promoting blood circulation and removing blood stasis, is widely applied in improving human erythrocyte deformability in clinics. But its direct effect on erythrocyte membranes is still unclear.
  
  
  METHODS
  In this essay, the confocal Raman technique was adopted to measure the changes in Raman spectra of human erythrocytes before and after the administration of Danshen injection.
  
  
  RESULTS
  The results showed slight changes in group conformations corresponding to erythrocyte membranes after the administration of danshen injection. Specifically, 1064, 1126 cm(-1) spectral lines attributed to phospholipid molecule acyl C-C skeleton anti-conformation were obviously weakened, whereas 1091 cm(-1) spectral line attributed to phospholipid molecule acyl C-C skeleton guache conformation notably intensified. Besides, the longitudinal order-parameter in chains (Strans) of phospholipids was reduced significantly.
  
  
  CONCLUSIONS
  Danshen injection can transfer erythrocyte membrane phospholipid molecule acyl C-C skeleton anti-conformation to guache conformation, indicating the increase in liquidity of erythrocyte membrane phospholipids and erythrocytes. Danshen injection's effect in improving erythrocyte membrane structure and function may be the intrinsic mechanism for its pharmacological effect as a traditional Chinese medicine.
authors.affiliation: Laboratory of Photonic Chinese Medicine, South China Normal University, Guangzhou 510631, China. chqyjck@163.com
authors.forename: Heqing
authors.initials: H
authors.lastname: Cai
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhao
authors.forename: Welling
authors.initials: W
authors.lastname: Zhu
authors.forename: Chao
authors.initials: C
authors.lastname: Yu
authors.forename: Shu
authors.initials: S
authors.lastname: Ge
authors.forename: Songhao
authors.initials: S
authors.lastname: Liu
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000737:chemistry; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects*; D005260:Female; D006801:Humans; D007267:Injections; D008297:Male; D008968:Molecular Conformation; D010743:Phospholipids / Q000737:chemistry; D027561:Salvia miltiorrhiza; D013059:Spectrum Analysis, Raman / Q000379:methods*; D055815:Young Adult
chemical_list: D001798:Blood Proteins; D010743:Phospholipids