title: Preparation and evaluation of 99mTc-cefuroxime, a potential infection specific imaging agent: a reliable thin layer chromatographic system to delineate impurities from the 99mTc-antibiotic.
abstract: Technetium-99m labelled cefuroxime, a second-generation cephalosporin antibiotic and potential bacteria specific infection imaging agent was evaluated. A good radiochemical purity (95%) of the labelled product was obtained after filtering the reaction mixture through a 0.22 μm filter. Scintigraphy study of the purified product showed uptake in infectious lesions 45 min after injection and abscess-to-muscle ratios were found to be 1.80, 1.85 and 1.88 at 45 min, 1.5 hr and 3 hr, respectively. A versatile and reliable chromatographic technique to assess the radiochemical purity of (99m)Tc-cefuroxime has also been described.
authors.affiliation: Radiopharmaceuticals Laboratory, Regional Centre, Board of Radiation and Isotope Technology (BRIT), Variable Energy Cyclotron Centre (VECC), Bidhan Nagar, Kolkata, India. sankha@vecc.gov.in
authors.forename: Sankha
authors.initials: S
authors.lastname: Chattopadhyay
authors.forename: Mayuri
authors.initials: M
authors.lastname: Ghosh
authors.forename: Sucharita
authors.initials: S
authors.lastname: Sett
authors.forename: Malay Kanti
authors.initials: MK
authors.lastname: Das
authors.forename: Susmita
authors.initials: S
authors.lastname: Chandra
authors.forename: Kakali
authors.initials: K
authors.lastname: De
authors.forename: Mridula
authors.initials: M
authors.lastname: Mishra
authors.forename: Samarendu
authors.initials: S
authors.lastname: Sinha
authors.forename: Bharat
authors.initials: B
authors.lastname: Ranjan Sarkar
authors.forename: Shantanu
authors.initials: S
authors.lastname: Ganguly
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002444:Cefuroxime / Q000737:chemistry*; D015609:Organotechnetium Compounds / Q000737:chemistry*; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
chemical_list: D000900:Anti-Bacterial Agents; D015609:Organotechnetium Compounds; D002444:Cefuroxime