title: Physiological and molecular responses of the earthworm (Eisenia fetida) to soil chlortetracycline contamination.
abstract: This study aims to evaluate toxic effects of exposure to chlortetracycline (CTC) in soil on reproductive endpoints (juvenile counts and cocoon counts), biochemical responses, and genotoxic potentials of the earthworm Eisenia fetida. Results showed that juvenile counts and cocoon counts of the tested earthworms were reduced after exposure to CTC. The effective concentrations (EC(50) values) for juvenile and cocoon counts were 96.1 and 120.3 mg/kg, respectively. Treatment of earthworms with CTC significantly changed the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST). An increase in malondialdehyde (MDA) indicated that CTC could cause cellular lipid peroxidation in the tested earthworms. The percentage of DNA in the tail of single-cell gel electrophoresis of coelomocytes as an indication of DNA damage increased after treatment with different doses of CTC, and a dose-dependent DNA damage of coelomocytes was found. In conclusion, CTC induces physiological responses and genotoxicity on earthworms.
authors.affiliation: Key Laboratory of Original Agro-environmental Quality, Ministry of Agriculture/Tianjin Key Laboratory of Agro-environment and Agro-product Safety, Institute of Agro-environmental Protection, Ministry of Agriculture, Tianjin 300191, China.
authors.forename: Dasong
authors.initials: D
authors.lastname: Lin
authors.forename: Qixing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Yingming
authors.initials: Y
authors.lastname: Xu
authors.forename: Chun
authors.initials: C
authors.lastname: Chen
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D002751:Chlortetracycline / Q000633:toxicity*; D005982:Glutathione Transferase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009835:Oligochaeta; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000633:toxicity*; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D012987:Soil; D012989:Soil Pollutants; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D002751:Chlortetracycline