title: NADPH oxidase-dependent oxidative stress and mitochondrial damage in hippocampus of D-galactose-induced aging rats.
abstract: Mitochondrial DNA (mtDNA) common deletion (CD) plays a significant role in aging and age-related diseases. In this study, we used D-galactose (D-gal) to generate an animal model of aging and the involvement and causative mechanisms of mitochondrial damage in such a model were investigated. Twenty 5-week-old male Sprague-Dawley rats were randomly divided into two groups: D-gal group (n=10) and control group (n=10). The quantity of the mtDNA CD in the hippocampus was determined using a TaqMan real-time PCR assay. Transmission electron microscopy was used to observe the mitochondrial ultrastructure in the hippocampus. Western blot was used to detect the protein levels of NADPH oxidase (NOX) and uncoupling protein 2 (UCP2). We found that the level of mtDNA CD was significantly higher in the hippocampus of D-gal-induced aging rats than in control rats. In comparison with the control group, the mitochondrial ultrastructure in the hippocampus of D-gal-treated rats was damaged, and the protein levels of NOX and UCP2 were significantly increased in the hippocampus of D-gal-induced aging rats. This study demonstrated that the levels of mtDNA CD and NOX protein expression were significantly increased in the hippocampus of D-gal-induced aging rats. These findings indicate that NOX-dependent reactive oxygen species generation may contribute to D-gal-induced mitochondrial damage.
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, 430022, China.|Department of Otorhinolaryngology, Shenzhen Sixth People's Hospital (Nanshan Hospital), Huazhong University of Science and Technology, Shenzhen, 518052, China.
authors.forename: Zhengde
authors.initials: Z
authors.lastname: Du
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Yujuan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Sulin
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Otorhinolaryngology, Huazhong University of Science and Technology, Wuhan, 430022, China. entwjkong@yahoo.com.cn.
authors.forename: Weijia
authors.initials: W
authors.lastname: Kong
authors.affiliation: Department of Public Health, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. zhl_bjk@whuh.com.
authors.forename: Honglian
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D005690:Galactose / Q000009:adverse effects* / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000378:metabolism* / Q000502:physiology; D019255:NADPH Oxidases / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019255:NADPH Oxidases; D005690:Galactose