title: Glycolipid biosurfactants, mannosylerythritol lipids, show antioxidant and protective effects against H(2)O(2)-induced oxidative stress in cultured human skin fibroblasts.
abstract: Mannosylerythritol lipids (MELs) are biosurfactants known for their versatile interfacial and biochemical properties. To broaden their application in cosmetics, we investigated the antioxidant properties of different MEL derivatives (MEL-A, -B, and -C) by using a 1,1-diphenyl-2-picryl hydrazine (DPPH) free-radical- and superoxide anion-scavenging assay. All MEL derivatives tested showed antioxidant activity in vitro, but at lower levels than those of arbutin. Of the MELs, MEL-C, which is produced from soybean oil by Pseudozyma hubeiensis, showed the highest rates of DPPH radical scavenging (50.3% at 10 mg/mL) and superoxide anion scavenging (>50% at 1 mg/mL). The antioxidant property of MEL-C was further examined using cultured human skin fibroblasts (NB1RGB cells) under H(2)O(2) induced oxidative stress. Surprisingly, MEL-C had a higher protective activity against oxidative stress than arbutin did: 10 µg/mL of MEL-C and arbutin had protective activities of 30.3% and 13%, respectively. Expression of an oxidative stress marker, cyclooxygenase-2, in these cells was repressed by treatment with MEL-C as well as by arbutin. MEL-C was thus confirmed to have antioxidant and protective effects in cells, and we suggest that MELs have potential as anti-aging skin care ingredients.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central, 5-2 Tsukuba Central, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Takahashi
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imura
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D001713:Biphenyl Compounds / Q000378:metabolism; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000378:metabolism; D019610:Cytoprotection / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006017:Glycolipids / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010851:Picrates / Q000378:metabolism; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000473:pathology; D013481:Superoxides / Q000378:metabolism; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology*; D014587:Ustilaginales / Q000737:chemistry
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D006017:Glycolipids; D010851:Picrates; D020011:Protective Agents; D013501:Surface-Active Agents; C439671:mannosylerythritol lipid; D013481:Superoxides; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human