title: Characterization of crystals of an antibody-recognition fragment of the cancer differentiation antigen mesothelin in complex with the therapeutic antibody MORAb-009.
abstract: The mesothelin-specific monoclonal antibody MORAb-009 is capable of blocking the binding of mesothelin to CA-125 and displays promising anticancer potential. It is currently undergoing clinical trials. In order to understand the basis of the interaction between MORAb-009 and mesothelin at atomic resolution, both the Fab fragment of MORAb-009 and the complex between the Fab and an N-terminal fragment of mesothelin (residues 7-64) were crystallized. The crystals of the Fab diffracted X-rays to 1.75 Å resolution and had the symmetry of space group P4(1)2(1)2, with unit-cell parameters a = b = 140.6, c = 282.0 Å. The crystals of the mesothelin-Fab complex diffracted to 2.6 Å resolution and belonged to the hexagonal space group P6(4), with unit-cell parameters a = b = 146.2, c = 80.9 Å. Structural analyses of these molecules are in progress.
authors.affiliation: Laboratory of Cell Biology, Center for Cancer Research, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Jichun
authors.initials: J
authors.lastname: Ma
authors.forename: Wai Kwan
authors.initials: WK
authors.lastname: Tang
authors.forename: Lothar
authors.initials: L
authors.lastname: Esser
authors.forename: Ira
authors.initials: I
authors.lastname: Pastan
authors.forename: Di
authors.initials: D
authors.lastname: Xia
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000737:chemistry* / Q000276:immunology; D003460:Crystallization; D058851:GPI-Linked Proteins / Q000737:chemistry* / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000276:immunology; D000090204:Mesothelin
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D058851:GPI-Linked Proteins; D007140:Immunoglobulin Fab Fragments; C526187:amatuximab; D000090204:Mesothelin