title: Double spiral microchannel for label-free tumor cell separation and enrichment.
abstract: This work reports on a passive double spiral microfluidic device allowing rapid and label-free tumor cell separation and enrichment from diluted peripheral whole blood, by exploiting the size-dependent hydrodynamic forces. A numerical model is developed to simulate the Dean flow inside the curved geometry and to track the particle/cell trajectories, which is validated against the experimental observations and serves as a theoretical foundation for optimizing the operating conditions. Results from separating tumor cells (MCF-7 and Hela) spiked into whole blood indicate that 92.28% of blood cells and 96.77% of tumor cells are collected at the inner and the middle outlet, respectively, with 88.5% tumor recovery rate at a throughput of 3.33 × 10(7) cells min(-1). We expect that this label-free microfluidic platform, driven by purely hydrodynamic forces, would have an impact on fundamental and clinical studies of circulating tumor cells.
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for NanoScience and Technology, Beijing, 100190, China. sunjs@nanoctr.cn
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dingbin
authors.initials: D
authors.lastname: Liu
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.forename: Guoqing
authors.initials: G
authors.lastname: Hu
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D057446:Hydrodynamics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D008962:Models, Theoretical; D009369:Neoplasms / Q000097:blood*