title: Real-time label-free monitoring of the cellular response to osmotic stress using conventional and long-range surface plasmons.
abstract: Cell volume and its regulation are key factors for cellular integrity and also serve as indicators of various cell pathologies. SPR sensors represent an efficient tool for real-time and label-free observations of changes in cell volume and shape. Here, we extend this concept by employing the use of long-range surface plasmons (LRSP). Due to the enhanced penetration depth of LRSP (~1μm, compared to ~0.4μm of a conventional surface plasmon), the observation of refractive index changes occurring deeper inside the cells is possible. In this work, the responses of a confluent normal rat kidney (NRK) epithelial cell layer to osmotic stress are studied by both conventional and long-range surface plasmons. Experiments are conducted in parallel using cell layers grown and stimulated under the same conditions to enable direct comparison of the results and discrimination of the osmotic stress-induced effects in different parts of the cell.
authors.affiliation: Institute of Photonics and Electronics, ASCR, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Vala
authors.forename: R
authors.initials: R
authors.lastname: Robelek
authors.forename: M
authors.initials: M
authors.lastname: Bocková
authors.forename: J
authors.initials: J
authors.lastname: Wegener
authors.forename: J
authors.initials: J
authors.lastname: Homola
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002460:Cell Line; D048429:Cell Size; D003199:Computer Systems; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007668:Kidney / Q000166:cytology* / Q000502:physiology*; D009997:Osmotic Pressure; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance / Q000295:instrumentation*