title: Characterization of Staphylococcus aureus strains isolated from Italian dairy products by MALDI-TOF mass fingerprinting.
abstract: Staphylococcus aureus is a known pathogen, causing serious food-borne intoxications due to the production of enterotoxins, being otherwise a major cause of mastitis. In this sense, the detection of S. aureus is an important issue for the food industry to avoid health hazards and economic losses. The present work applied MALDI-TOF MS for the classification of 40 S. aureus strains, 36 isolated from Italian dairy products and four from human samples. All isolated strains were clearly identified as S. aureus by their spectral fingerprints. The peak masses m/z 3444, 5031, and 6887 were determined to be specific biomarkers for S. aureus. Furthermore, clustering of the peak mass lists was successfully applied as a typing method, resulting in eight groups of strains. This is the first time that a detailed spectral comparison was carried out and characteristic peak masses were determined for every spectral group. Three strains exhibited a peak at m/z 6917 instead of m/z 6887, which was related to four polymorphisms in their 16S rRNA sequences. However, the grouping obtained by MALDI-TOF MS fingerprinting could not be related to toxin production or to the origin of the strains.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, Lugo, Spain.
authors.forename: Karola
authors.initials: K
authors.lastname: Böhme
authors.forename: Stefano
authors.initials: S
authors.lastname: Morandi
authors.forename: Paola
authors.initials: P
authors.lastname: Cremonesi
authors.forename: Inmaculada C
authors.initials: IC
authors.lastname: Fernández No
authors.forename: Jorge
authors.initials: J
authors.lastname: Barros-Velázquez
authors.forename: Bianca
authors.initials: B
authors.lastname: Castiglioni
authors.forename: Milena
authors.initials: M
authors.lastname: Brasca
authors.forename: Benito
authors.initials: B
authors.lastname: Cañas
authors.forename: Pilar
authors.initials: P
authors.lastname: Calo-Mata
mesh_terms: D016000:Cluster Analysis; D003611:Dairy Products / Q000382:microbiology*; D059022:Food Safety; D006801:Humans; D007558:Italy; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000145:classification / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S