title: Antimutagenic properties of Mangifera indica L. stem bark extract and evaluation of its effects on hepatic CYP1A1.
abstract: Mangifera indica stem bark extract (MSBE) is a Cuban natural product which has shown strong antioxidant properties. In this work, the antimutagenic effect of MSBE was tested against 10 well-known mutagens/carcinogens in the Ames test in the absence or presence of metabolic fraction (S9). The chemical mutagens tested included: cyclophosphamide, mitomycin C, bleomycin, cisplatin, dimethylnitrosamine (DMNA), benzo[a]pyrene (BP), 2-acetylaminofluorene (2-AAF), sodium azide, 1-nitropyrene (1-NP) and picrolonic acid. Protective effects of the extract were also evaluated by comparing the efficiency of S9 fraction obtained from rats treated during 28 days with oral doses of MSBE (50-500 mg/kg) with that obtained from rats treated with vehicle (control) to activate bleomycin and cyclophosphamide in the Ames test. MSBE concentrations between 50 and 500 μg/plate significantly reduced the mutagenicity mediated by all the chemicals tested with the exception of sodium azide. Higher mutagenicity was found when bleomycin and cyclophosphamide (CP) were activated by control S9 than by MSBE S9. In addition, inhibition of CYP1A1 microsomal activity was observed in the presence of MSBE (10-20 μg/ml). We can conclude that besides its potent antioxidant activity previously reported, MSBE may also exert a chemoprotective effect due to its capacity to inhibit CYP activity.
authors.affiliation: Universidad de la Habana, 222 st., La Coronela, La Habana, Cuba.
authors.forename: Janet
authors.initials: J
authors.lastname: Morffi
authors.forename: Idania
authors.initials: I
authors.lastname: Rodeiro
authors.forename: Sandra Luz
authors.initials: SL
authors.lastname: Hernández
authors.forename: Leonora
authors.initials: L
authors.lastname: González
authors.forename: Jose
authors.initials: J
authors.lastname: Herrera
authors.forename: J Javier
authors.initials: JJ
authors.lastname: Espinosa-Aguirre
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001761:Bleomycin; D002273:Carcinogens; D003462:Cuba; D003520:Cyclophosphamide; D019363:Cytochrome P-450 CYP1A1 / Q000037:antagonists & inhibitors*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D031022:Mangifera; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D009152:Mutagenicity Tests; D009153:Mutagens; D024301:Plant Bark; D010936:Plant Extracts / Q000494:pharmacology*; D018547:Plant Stems; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; D002273:Carcinogens; C501277:Mangifera indica extract; D009153:Mutagens; D010936:Plant Extracts; D001761:Bleomycin; D003520:Cyclophosphamide; D019363:Cytochrome P-450 CYP1A1