title: Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos.
abstract: This study documents the spatial and temporal distribution of Oct-4, Cdx-2 and acetylated H4K5 (H4K5ac) by immunocytochemistry staining using in-vivo-derived rabbit embryos at different stages: day-3 compact morulae, day-4 early blastocysts, day-4 expanded blastocysts, day-5 blastocysts, day-6 blastocysts and day-7 blastocysts. The Oct-4 signal was stronger in the inner cell mass (ICM)/epiblast cells than in the trophectoderm (TE) cells in all blastocyst stages except day-4 expanded blastocysts, where the signal was similarly weak in both the ICM and TE cells. The Cdx-2 signal was first detected in a small number of TE cells of day-4 early blastocysts, and became evident in the TE cells exclusively afterwards. A consistently strong H4K5ac signal was observed in the TE cells in all blastocyst stages examined. In particular, this signal was stronger in the TE than in the ICM cells in day-4 early blastocysts, day-4 expanded blastocysts and day-5 blastocysts. Double staining of H4K5ac with either Oct-4 or Cdx-2 on embryos at different blastocyst stages confirmed these findings. This work suggests that day 4 is a critical timing for lineage formation in rabbit embryos. A combination of Oct-4, Cdx-2 and H4K5ac can be used as biomarkers to identify different lineage cells in rabbit blastocysts.
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei, Taiwan, ROC.
authors.forename: Chien-Hong
authors.initials: CH
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Chang
authors.forename: Chia-Chia
authors.initials: CC
authors.lastname: Liu
authors.forename: Hwa-Yun
authors.initials: HY
authors.lastname: Su
authors.forename: Y Eugene
authors.initials: YE
authors.lastname: Chen
authors.forename: Fuliang
authors.initials: F
authors.lastname: Du
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Sung
mesh_terms: D000107:Acetylation; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D053624:Blastocyst Inner Cell Mass / Q000166:cytology / Q000378:metabolism; D000071616:CDX2 Transcription Factor; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D047108:Embryonic Development; D005260:Female; D006657:Histones / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism*; D007150:Immunohistochemistry; D008239:Lysine / Q000378:metabolism; D018613:Microscopy, Confocal; D009028:Morula / Q000166:cytology / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000378:metabolism*; D011247:Pregnancy; D011817:Rabbits / Q000196:embryology* / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D000071616:CDX2 Transcription Factor; D006657:Histones; D018398:Homeodomain Proteins; D050814:Octamer Transcription Factor-3; D015534:Trans-Activators; D008239:Lysine