title: Selection of human VH single domains with improved biophysical properties by phage display.
abstract: Human antibody variable heavy (VH) domains tend to display poor biophysical properties when expressed in isolation. Consequently, the domains are often characterized by low expression levels, high levels of aggregation, and increased "stickiness." Here, we describe methods that allow the engineering of human VH domains with improved biophysical properties by phage display. The engineered domains withstand challenging conditions, such as high temperature and acidic pH. Engineered human single domains are a promising new class of antibody fragments and represent robust research tools and building blocks for the generation of antibody therapeutics.
authors.affiliation: Garvan Institute of Medical Research, Darlinghurst/Sydney, NSW, Australia.
authors.forename: Kip
authors.initials: K
authors.lastname: Dudgeon
authors.forename: Romain
authors.initials: R
authors.lastname: Rouet
authors.forename: Kristoffer
authors.initials: K
authors.lastname: Famm
authors.forename: Daniel
authors.initials: D
authors.lastname: Christ
mesh_terms: D061505:Cell Surface Display Techniques / Q000379:methods*; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017510:Protein Folding; D061905:Single-Domain Antibodies / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D012995:Solubility; D013696:Temperature
chemical_list: D061905:Single-Domain Antibodies