title: Chromatographic separation studies of penicillins, cephalosporins and carbapenems on transition-metal silicate modified silica layers.
abstract: The chromatographic behavior of penicillins, cephalosporins and carbapenems has been studied on the thin layers of transition-metal ion (viz. Ni(2+)/Zn(2+)/Cu(2+)/Co(2+)) silicate modified silica. Transition-metal silicate (3.92%) and silica (96.08%) were found to be optimum and resulted in spherical-compact spots and improved resolution of the analytes. The effect of various mobile phases was also investigated. The chromatograms were visualized as yellow spots by placing in an I(2)-chamber. The method has been found to be reproducible and convenient for routine analysis.
authors.affiliation: Analytical Research Laboratory, Department of Chemistry, Harcourt Butler Technological Institute, Kanpur, India. dhruvks123@rediffmail.com
authors.forename: Dhruv K
authors.initials: DK
authors.lastname: Singh
authors.forename: Gunjan
authors.initials: G
authors.lastname: Maheshwari
mesh_terms: D015780:Carbapenems / Q000737:chemistry / Q000302:isolation & purification*; D002511:Cephalosporins / Q000737:chemistry / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer / Q000295:instrumentation; D010406:Penicillins / Q000737:chemistry / Q000302:isolation & purification*; D017640:Silicates / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D028561:Transition Elements / Q000737:chemistry*
chemical_list: D015780:Carbapenems; D002511:Cephalosporins; D010406:Penicillins; D017640:Silicates; D028561:Transition Elements; D012822:Silicon Dioxide