title: A multiparametric assay to compare the cytotoxicity of soy milk with different storage media.
abstract: OBJECTIVE
  The aim of this study was to evaluate the cytotoxicity of soy milk compared with several other storage media [coconut water, Hank's Balanced Salt Solution (HBSS) and whole milk], assessed through a multiparametric analysis employing 3T3 cells.
  
  
  METHODS
  Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24 h, at 37°C with 5% CO₂, and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). Results from each test were compared by two-way analysis of variance (ANOVA).
  
  
  RESULTS
  Statistical analysis showed that whole milk, HBSS and soy milk were the most effective media in maintaining cell viability at all tested times (P < 0.05). The least amount of viable cells was observed when using coconut water.
  
  
  CONCLUSIONS
  This study shows that the efficacy of soy milk in maintaining the viability of 3T3 fibroblasts is similar to that of HBSS and milk, as shown by three different cell viability tests.
authors.affiliation: Endodontic Division, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil. nogueiraemmanuel@hotmail.com
authors.forename: Emmanuel J N L
authors.initials: EJ
authors.lastname: Silva
authors.forename: Carolina B
authors.initials: CB
authors.lastname: Rollemberg
authors.forename: Tauby
authors.initials: T
authors.lastname: de Souza Coutinho-Filho
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Zaia
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D041702:BALB 3T3 Cells; D002452:Cell Count; D002470:Cell Survival; D002478:Cells, Cultured; D003059:Cocos / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D007202:Indicators and Reagents; D007552:Isotonic Solutions / Q000737:chemistry*; D051379:Mice; D008892:Milk / Q000737:chemistry*; D019188:Organ Preservation Solutions / Q000737:chemistry*; D041942:Soy Milk / Q000737:chemistry*; D013778:Tetrazolium Salts
chemical_list: C072670:Hanks Balanced Salt Solution; D007202:Indicators and Reagents; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide