title: Protection of lung fibroblast MRC-5 cells against hydrogen peroxide-induced oxidative damage by 0.1-2.8 kDa antioxidative peptides isolated from whey protein hydrolysate.
abstract: Antioxidative peptides (0.1-2.8 kDa) obtained from gel filtration of Alcalase-hydrolysed whey protein were subjected to individual peptide isolation by RP-HPLC. The sub-fraction P4 and a prominent pentapeptide identified by mass spectrometry (Val-His-Leu-Lys-Pro) (P4c) were found to be highly antioxidative, therefore, used to assess the efficacy against hydrogen peroxide (H(2)O(2))-induced human lung fibroblast MRC-5 cell oxidative injury. MRC-5 cells were damaged by incubation with H(2)O(2), but cell death was significantly reduced when exposed to P4 and P4c (P<0.05). Compared to the H(2)O(2)-damaged model control, P4 and P4c enhanced the activity of cell superoxide dismutase, glutathione peroxidase, and catalase, and decreased the levels of malonaldehyde (P<0.05). These results, with a more pronounced action of P4c than P4, demonstrated that antioxidative peptides had a significant protection of MRC-5 cells against the toxicity caused by H(2)O(2).
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Kong
authors.forename: Xinyan
authors.initials: X
authors.lastname: Peng
authors.forename: Youling L
authors.initials: YL
authors.lastname: Xiong
authors.forename: Xinhuai
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008894:Milk Proteins / Q000737:chemistry / Q000494:pharmacology*; D008970:Molecular Weight; D018384:Oxidative Stress / Q000187:drug effects*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology*; D000067816:Whey Proteins
chemical_list: D000975:Antioxidants; D008894:Milk Proteins; D010455:Peptides; D020011:Protective Agents; D011492:Protein Hydrolysates; D000067816:Whey Proteins; D006861:Hydrogen Peroxide