title: Quantitative determination of fatty acid compositions in micro-encapsulated fish-oil supplements using Fourier transform infrared (FTIR) spectroscopy.
abstract: The research describes a rapid method for the determination of fatty acid (FA) contents in a micro-encapsulated fish-oil (μEFO) supplement by using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic technique and partial least square regression (PLSR) analysis. Using the ATR-FTIR technique, the μEFO powder samples can be directly analysed without any pre-treatment required, and our developed PLSR strategic approach based on the acquired spectral data led to production of a good linear calibration with R(2)=0.99. In addition, the subsequent predictions acquired from an independent validation set for the target FA compositions (i.e., total oil, total omega-3 fatty acids, EPA and DHA) were highly accurate when compared to the actual values obtained from standard GC-based technique, with plots between predicted versus actual values resulting in excellent linear fitting (R(2)≥0.96) in all cases. The study therefore demonstrated not only the substantial advantage of the ATR-FTIR technique in terms of rapidness and cost effectiveness, but also its potential application as a rapid, potentially automated, online monitoring technique for the routine analysis of FA composition in industrial processes when used together with the multivariate data analysis modelling.
authors.affiliation: School of Life and Environmental Sciences, Deakin University, Pigdons Road, Waurn Ponds, Victoria 3217, Australia. p.vongsvivut@deakin.edu.au
authors.forename: Jitraporn
authors.initials: J
authors.lastname: Vongsvivut
authors.forename: Philip
authors.initials: P
authors.lastname: Heraud
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jaroslav A
authors.initials: JA
authors.lastname: Kralovec
authors.forename: Don
authors.initials: D
authors.lastname: McNaughton
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Barrow
mesh_terms: D019587:Dietary Supplements / Q000032:analysis*; D005227:Fatty Acids / Q000737:chemistry*; D005395:Fish Oils / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D005227:Fatty Acids; D005395:Fish Oils