title: [Screening and identification of Polo-like kinase 1 as a 14-3-3Zeta-interacting protein].
abstract: OBJECTIVE
  To screen 14-3-3Zeta-interacting proteins by the yeast two-hybrid system and confirm the interaction between 14-3-3Zeta and Polo-like kinase 1 (Plk1).
  
  
  METHODS
  A bait vector pGBKT7-14-3-3Zeta was constructed to screen the 14-3-3Zeta-interacting proteins from the HeLa cDNA library by yeast two-hybrid system. The interaction between 14-3-3Zeta and Plk1 was further confirmed by immunoprecipitation assay. We also examined the distribution of endogenous 14-3-3Zeta and Plk1 in mitosis using immunofluorescent staining.
  
  
  RESULTS
  Plk1 was identified as a partner of 14-3-3Zeta protein by the yeast two-hybrid system. Immunoprecipitation assay demonstrated the interaction between 14-3-3Zeta and Plk1. Immunofluorescence indicated 14-3-3Zeta was colocalized with Plk1 at the midbody during cytokinesis.
  
  
  CONCLUSIONS
  Plk1 is a serine/threonine protein kinase that plays multiple critical roles in centrosome maturation, mitotic chromosome segregation, cytokinesis, and the DNA damage response. The interaction between 14-3-3Zeta and Plk1 suggests 14-3-3 family emerges as a novel player in the mitotic regulation.
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Basic Medicine, Hefei, China. dujane@163.com
authors.forename: Jian
authors.initials: J
authors.lastname: Du
authors.forename: Li
authors.initials: L
authors.lastname: Cheng
authors.forename: Li-jian
authors.initials: LJ
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Ran
authors.initials: R
authors.lastname: An
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D048749:Cytokinesis; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D000097869:Polo-Like Kinase 1
chemical_list: D048948:14-3-3 Proteins; D018797:Cell Cycle Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1