title: Crystal structure of a major seed storage protein, 11S proglobulin, from Amaranthus hypochondriacus: insight into its physico-chemical properties.
abstract: Amaranth is a crop known for its high quality proteins. 11S Globulin is one of the most abundant and important storage proteins of the amaranth grain. Here, we report the crystal structure of amaranth 11S proglobulin at a final resolution of 2.28 Å. It belonged to the space group P6(3) with cell dimensions a=b=96.6, c=75.0 Å. It contains one asymmetric unit consisting of 372 residues and 100 water molecules. Disordered regions in the model approximately correspond to the variable regions of the 11S globulins. The structure has an extended α-helix and β-barrel domains at both N-terminal and C-terminal regions, which are characteristic of the 11S and 7S globulins. The three dimensional structure suggests that its high thermal stability is due to the cumulative effects of many factors and its good emulsifying property depended on the balance between its surface hydrophobicity and hydrophilicity.
authors.affiliation: Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Mary Rose
authors.initials: MR
authors.lastname: Tandang-Silvas
authors.forename: Cerrone S
authors.initials: CS
authors.lastname: Cabanos
authors.forename: Laura Denisse
authors.initials: LD
authors.lastname: Carrazco Peña
authors.forename: Ana Paulina Barba
authors.initials: AP
authors.lastname: De la Rosa
authors.forename: Juan Alberto
authors.initials: JA
authors.lastname: Osuna-Castro
authors.forename: Shigeru
authors.initials: S
authors.lastname: Utsumi
authors.forename: Bunzo
authors.initials: B
authors.lastname: Mikami
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Maruyama
mesh_terms: D027721:Amaranthus / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D055598:Chemical Phenomena; D003460:Crystallization; D005916:Globulins / Q000737:chemistry* / Q000235:genetics; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics; D055550:Protein Stability; D017433:Protein Structure, Secondary; D055314:Seed Storage Proteins / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D005916:Globulins; D011498:Protein Precursors; D055314:Seed Storage Proteins