title: Tissue factor pathway inhibitor-2 inhibits the growth and invasion of hepatocellular carcinoma cells and is inactivated in human hepatocellular carcinoma.
abstract: Human tissue factor pathway inhibitor-2 (TFPI-2) is an extracellular matrix-associated Kunitz-type serine proteinase inhibitor that inhibits the plasmin- and trypsin-mediated activation of matrix metalloproteinases and inhibits tumor progression, invasion and metastasis. Previous studies have shown that TFPI-2 is downregulated in the progression of various tumors. The purpose of this study was to investigate the expression and function of TFPI-2 in hepatocellular carcinoma (HCC). In situ hybridization was used to detect human TFPI-2 mRNA and immunohistochemistry was performed to examine the role of TFPI-2 expression in hepatocarcinoma tissues. Cell proliferation was assessed using MTT assay. In situ hybridization and immunohistochemical analyses revealed that the expression of TFPI-2 in hepatocarcinoma tissues was markedly lower than that in tumor-adjacent normal hepatic tissues. Restored expression of TFPI-2 in HepG(2) cells inhibits cell proliferation and invasion. Taken together, the results suggest that TFPI-2 has a tumor-suppression action and its inactivation may contribute to HCC.
authors.affiliation: Pingshan People's Hospital, Pingshan, Shenzhen, Guangdong 518118, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Qin
authors.forename: Jinjing
authors.initials: J
authors.lastname: Zhou
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Tu
authors.forename: Xiao
authors.initials: X
authors.lastname: Bi
authors.forename: Wuxian
authors.initials: W
authors.lastname: Li
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Fan
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang