title: Effects of local delivery of bFGF from PLGA microspheres on osseointegration around implants in diabetic rats.
abstract: OBJECTIVE
  Diabetes mellitus may impair bone healing after dental implant placement. The objective of this study was to evaluate the effects of the local delivery of basic fibroblast growth factor (bFGF) from poly(lactide-co-glycolide) (PLGA) microspheres on osseointegration around titanium implants in diabetic rats.
  
  
  METHODS
  The bFGF-PLGA microspheres were prepared by the W/O/W double-emulsion solvent evaporation method. A total of 20 rats were used to create diabetic animal models by giving them a high-fat and high-sugar diet and a low-dose streptozotocin intraperitoneal injection. Titanium implants were planted into the tibias of the diabetic rats and into 10 normal rats. Microspheres were loaded on the surfaces of the implants in the bFGF intervention group before they were placed into the rats. After 4 or 8 weeks, the tibias containing the implants were removed and embedded with resin. Uncalcified tissue slices were prepared to compare osseointegration.
  
  
  RESULTS
  At 4 weeks, the bone-implant contact rate in the diabetic control group was less than that in the control group and the bFGF intervention group (P < .05). At 8 weeks, the results among the 3 groups were similar to those at 4 weeks.
  
  
  CONCLUSIONS
  The local delivery of bFGF from PLGA microspheres into areas around titanium implants may improve osseointegration in diabetic rats.
authors.affiliation: Department of Oral Implantology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Gui-Ke
authors.initials: GK
authors.lastname: Zou
authors.forename: Ying-Liang
authors.initials: YL
authors.lastname: Song
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Miao
authors.initials: M
authors.lastname: Yu
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Liang
authors.forename: Dao-Cai
authors.initials: DC
authors.lastname: Sun
authors.forename: De-Hua
authors.initials: DH
authors.lastname: Li
authors.forename: Zai-Xi
authors.initials: ZX
authors.lastname: Deng
authors.forename: Wen-Zhong
authors.initials: WZ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D004195:Disease Models, Animal; D004337:Drug Carriers; D016503:Drug Delivery Systems; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D005856:Germ-Free Life; D019344:Lactic Acid; D008297:Male; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D016348:Osseointegration / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D013499:Surface Properties; D013977:Tibia / Q000601:surgery; D014025:Titanium / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D015921:Dental Implants; D003764:Dental Materials; D004337:Drug Carriers; D016222:Fibroblast Growth Factor 2; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D013311:Streptozocin; D014025:Titanium