title: The first report of the qnrB19, qnrS1 and aac(6´)-Ib-cr genes in urinary isolates of ciprofloxacin-resistant Escherichia coli in Brazil.
abstract: In this study, we investigated the presence of plasmid-mediated quinolone resistance (PMQR) genes among 101 ciprofloxacin-resistant urinary Escherichia coli isolates and searched for mutations in the quinolone-resistance-determining regions (QRDRs) of the DNA gyrase and topoisomerase IV genes in PMQR-carrying isolates. Eight isolates harboured the qnr and aac(6')-Ib-cr genes (3 qnrS1, 1 qnrB19 and 4 aac(6')-Ib-cr). A mutational analysis of the QRDRs in qnr and aac(6')-Ib-cr-positive isolates revealed mutations in gyrA, parC and parE that might be associated with high levels of resistance to quinolones. No mutation was detected in gyrB. Rare gyrA, parC and parE mutations were detected outside of the QRDRs. This is the first report of qnrB19, qnrS1 and aac(6')-Ib-cr -carrying E. coli isolates in Brazil.
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil, 31270-901.
authors.forename: Magna Cristina
authors.initials: MC
authors.lastname: Paiva
authors.forename: Andréa Maria Amaral
authors.initials: AM
authors.lastname: Nascimento
authors.forename: Ilana Lopes Baratella Cunha
authors.initials: IL
authors.lastname: Camargo
authors.forename: Cláudia Iracema
authors.initials: CI
authors.lastname: Lima-Bittencourt
authors.forename: Regina Maria Drummond
authors.initials: RM
authors.lastname: Nardi
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase; D027101:DNA Topoisomerase IV; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005260:Female; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D015363:Quinolones / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; C485384:Qnr protein, E coli; D015363:Quinolones; D002939:Ciprofloxacin; D000123:Acetyltransferases; C056532:aminoglycoside N(6')-acetyltransferase; D027101:DNA Topoisomerase IV; D027081:DNA Gyrase