title: [A Bw12 blood type caused by 278C>T mutation of ABO gene].
abstract: OBJECTIVE
  To determine the serotype and genotype of a sample with ABO blood group discrepancies.
  
  
  METHODS
  Serotype was determined with serological method. Sequence specific primer polymerase chain reaction (SSP-PCR) was carried out based on the serotype. Sequences of exons 6 and 7 of ABO gene was analyzed by sequence-based testing (SBT).
  
  
  RESULTS
  Completely agglutinated A antigen, half agglutinated B antigen and weak agglutinated anti-B antibody were detected in both erythrocytes and serum, which suggested presence of a ABw serotype. An A/Bw12 genotype was revealed by B subgroup detection. Sequences of exons 6 and 7 were 278CT, 297GA and 467CT, 526CG, 657CT, 703GA, 796CA, 803GC, 930GA, respectively. The genotype fit with A102/B101 except for a nt278 C>T mutation. Blood group antigen gene mutation database (BGMUT) search has confirmed the mutant allele to be Bw12.
  
  
  CONCLUSIONS
  An A102/Bw12 genotype has been found in the Chinese population.
authors.affiliation: Dalian Blood Center, Dalian, Liaoning, People's Republic of China. mengqingli@dl.cn
authors.forename: Qing-li
authors.initials: QL
authors.lastname: Meng
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Duan
authors.forename: Ling-zi
authors.initials: LZ
authors.lastname: Pan
authors.forename: Wei-jian
authors.initials: WJ
authors.lastname: Yu
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D001483:Base Sequence; D001789:Blood Group Antigens / Q000235:genetics*; D001788:Blood Grouping and Crossmatching / Q000379:methods; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation
chemical_list: D000017:ABO Blood-Group System; D001789:Blood Group Antigens