title: Isolation of a novel strain of Monoraphidium sp. and characterization of its potential application as biodiesel feedstock.
abstract: A novel green microalgae strain from Lake Fuxian has been isolated and identified as a potential feedstock for biodiesel production. The novel strain was named Monoraphidium sp. FXY-10 based on its morphological and genomic characterization. The lipid productivities, fatty acid profiles, and microalgae recovery efficiency (η(a)) of FXY-10 were investigated and compared under autotrophic and heterotrophic conditions. FXY-10 under autotrophic conditions exhibited a higher cellular lipid content (56.8%) than those under heterotrophic conditions (37.56%). However, FXY-10 growing under heterotrophic conditions exhibited more than 20-fold increase in lipid productivity compared with that under autotrophic conditions (148.74 mg L(-1)d(-1) versus 6.88 mg L(-1)d(-1)). Moreover, higher saturated and monounsaturated fatty acids (77.5%) of FXY-10 was obtained under heterotrophic culture conditions, suggesting its potential as a biodiesel feedstock. Gravity sedimentation was proposed as the harvesting biomass method based on the 97.9% microalgae recovery efficiency of heterotrophic cells after settling for 24h.
authors.affiliation: Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming 650500, China.
authors.forename: Xuya
authors.initials: X
authors.lastname: Yu
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Cian
authors.initials: C
authors.lastname: He
authors.forename: Junjun
authors.initials: J
authors.lastname: Li
authors.forename: Xianhua
authors.initials: X
authors.lastname: Tang
authors.forename: Junpei
authors.initials: J
authors.lastname: Zhou
authors.forename: Zunxi
authors.initials: Z
authors.lastname: Huang
mesh_terms: D052818:Autotrophic Processes / Q000502:physiology; D001483:Base Sequence; D056804:Biofuels / Q000382:microbiology*; D002681:China; D000460:Chlorophyta / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis*; D052836:Heterotrophic Processes / Q000502:physiology; D060106:Lakes; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis*; D058086:Microalgae / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D019308:Palmitic Acid / Q000032:analysis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D056804:Biofuels; D017931:DNA Primers; D005227:Fatty Acids; D008055:Lipids; D012337:RNA, Ribosomal, 18S; D019308:Palmitic Acid