title: Tuberculosis caused by RDRio Mycobacterium tuberculosis is not associated with differential clinical features.
abstract: BACKGROUND
  We recently described the Mycobacterium tuberculosis RD(Rio) genotype, a clonally derived sublineage within the Latin American-Mediterranean (LAM) family. Genetic diversity of M. tuberculosis likely affects the clinical aspects of tuberculosis  (TB). Prospective studies that address this issue are scarce and remain controversial.
  
  
  OBJECTIVE
  To determine the association of differential clinical features of pulmonary TB with the RD(Rio) M. tuberculosis etiology.
  
  
  METHODS
  Culture-proven pulmonary TB patients (n  = 272) were clinically evaluated, including history, physical examination, chest X-ray and anti-human immunodeficiency virus serology. Isolates were classified as RD(Rio) or non-RD(Rio) M. tuberculosis by multiplex polymerase chain reaction and further spoligotyped.  Clinical and M. tuberculosis genotype data were analyzed.
  
  
  RESULTS
  RD(Rio) M. tuberculosis caused disease in 26.5% (72/270) of all TB cases. The LAM genotype, of which RD(Rio) strains are members, was responsible for 46.0% of the TB cases. Demographic data,  major signs and symptoms, radiographic presentation, microbiological features and clinical outcomes were not significantly different among patients with TB caused by RD(Rio) and non-RD(Rio) strains.
  
  
  CONCLUSIONS
  Disease caused by M. tuberculosis RD(Rio)  strains was not clinically distinctive or more severe than disease caused by non-RD(Rio) strains in this series of TB patients. Larger prospective studies specifically designed to disclose differential clinical characteristics of TB caused by specific M. tuberculosis lineages  are needed.
authors.affiliation: Multidisciplinary Research Laboratory, Clementino Fraga Filho University Hospital, Institute of Thoracic Diseases, Faculty of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: C de B
authors.initials: Cde B
authors.lastname: Barbosa
authors.forename: L C O
authors.initials: LC
authors.lastname: Lazzarini
authors.forename: A R
authors.initials: AR
authors.lastname: Elias
authors.forename: J A M
authors.initials: JA
authors.lastname: Leung
authors.forename: S B
authors.initials: SB
authors.lastname: Ribeiro
authors.forename: M G
authors.initials: MG
authors.lastname: da Silva
authors.forename: R S
authors.initials: RS
authors.lastname: Duarte
authors.forename: P
authors.initials: P
authors.lastname: Suffys
authors.forename: H M
authors.initials: HM
authors.lastname: Gomes
authors.forename: A L
authors.initials: AL
authors.lastname: Kritski
authors.forename: J R
authors.initials: JR
authors.lastname: Lapa E Silva
authors.forename: J L
authors.initials: JL
authors.lastname: Ho
authors.forename: N
authors.initials: N
authors.lastname: Boéchat
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001938:Brazil / Q000453:epidemiology; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial