title: Preparative purification of five bioactive components from Agrimonia pilosa Ledeb by high-speed counter-current chromatography.
abstract: High-speed counter-current chromatography (HSCCC) coupled with ultraviolet (UV) detection or evaporative light-scattering detection was successfully applied for preparative separation of five bioactive compounds from Agrimonia pilosa Ledeb. In preliminary process, D101 macroporous resin was used to separate the crude extract of the plant and four fractions (20, 40, 50, and 60% aqueous ethanol elutions) were produced. Then, these fractions were directly subjected to HSCCC purification. Five chemicals including taxifolin-3-glucoside (6.4 mg), quercetin-3-rhamnoside (13.0 mg), tiliroside (14.7 mg), agrimonolide (21.4 mg), and tormentic acid (29.8 mg) with the purities of 94.24, 95.37, 97.42, 95.29, and 96.34% were separated from each 200 mg prepared fraction. The purities were analyzed by high-performance liquid chromatography, and the chemical structures of the products were identified by UV detection, mass spectrometry, nuclear magnetic resonance, and the standards. This paper used a simple method to separate five bioactive compounds from A. pilosa Ledeb, and it could provide a new idea for the purification of bioactive compounds from other medicinal plants.
authors.affiliation: College of Pharmacy, Dalian Medical University, Dalian, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Mozhen
authors.initials: M
authors.lastname: Liu
authors.forename: Lingli
authors.initials: L
authors.lastname: Zheng
authors.forename: Lianhong
authors.initials: L
authors.lastname: Yin
authors.forename: Lina
authors.initials: L
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Qi
authors.forename: Xiaochi
authors.initials: X
authors.lastname: Ma
authors.forename: Kexin
authors.initials: K
authors.lastname: Liu
authors.forename: Jinyong
authors.initials: J
authors.lastname: Peng
mesh_terms: D031981:Agrimonia / Q000737:chemistry*; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D005960:Glucosides; D010936:Plant Extracts