title: [Determination of podophyllotoxin and total lignans in Sinopodophyllum emodi].
abstract: OBJECTIVE
  To determine the content of podophyllotoxin and total lignans in Sinopodophyllum emodi Ying from different areas and evaluate the resource utilization of this endangered medicinal plant.
  
  
  METHODS
  HPLC and UV spectrophotometry were used to determine the content of podophyllotoxin and total lignans in 126 samples from different habitats and the total lignans content was determined by the reference wavelength method.
  
  
  RESULTS
  According to the results, the highest content was determined from the samples from Yongdeng Nature Reserve in Gansu province, and the lowest ones was found in the samples from Tibet. The former's podophyllotoxin and total lignans contents were 7.40% and 20.66%, respectively, which were 19 times and 4 times more than those of the latter. The content of podophyllotoxin and total lignans in S. emodi were significantly positively related, meanwhile, samples from both low altitude and high latitude showed the higher content.
  
  
  CONCLUSIONS
  The two determination methods are simple, rapid, accurate and repeatable. It is more scientific and rational to evaluate the resource utilization of S. emodi with two indicators, those are the content of podophyllotoxin and the content of total lignans. This paper is instructive to the collection of wild resources and the establishment of production bases.
authors.affiliation: Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Jifeng
authors.initials: J
authors.lastname: Zhao
authors.forename: Changhua
authors.initials: C
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Songyun
authors.initials: S
authors.lastname: Qin
authors.forename: Guoyue
authors.initials: G
authors.lastname: Zhong
mesh_terms: D028141:Berberidaceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D017705:Lignans / Q000032:analysis*; D011034:Podophyllotoxin / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D017705:Lignans; D011034:Podophyllotoxin