title: Crystallization and preliminary X-ray diffraction analysis of three myotoxic phospholipases A2 from Bothrops brazili venom.
abstract: Two myotoxic and noncatalytic Lys49-phospholipases A(2) (braziliantoxin-II and MT-II) and a myotoxic and catalytic phospholipase A(2) (braziliantoxin-III) from the venom of the Amazonian snake Bothrops brazili were crystallized. The crystals diffracted to resolutions in the range 2.56-2.05 Å and belonged to space groups P3(1)21 (braziliantoxin-II), P6(5)22 (braziliantoxin-III) and P2(1) (MT-II). The structures were solved by molecular-replacement techniques. Both of the Lys49-phospholipases A(2) (braziliantoxin-II and MT-II) contained a dimer in the asymmetric unit, while the Asp49-phospholipase A(2) braziliantoxin-III contained a monomer in its asymmetric unit. Analysis of the quaternary assemblies of the braziliantoxin-II and MT-II structures using the PISA program indicated that both models have a dimeric conformation in solution. The same analysis of the braziliantoxin-III structure indicated that this protein does not dimerize in solution and probably acts as a monomer in vivo, similar to other snake-venom Asp49-phospholipases A(2).
authors.affiliation: Departamento de Física e Biofísica, Instituto de Biociências, UNESP - Universidade Estadual Paulista, Botucatu-SP and Instituto Nacional de Ciência e Tecnologia em Toxinas, CNPq, Brazil.
authors.forename: Carlos A H
authors.initials: CA
authors.lastname: Fernandes
authors.forename: Elaine C G
authors.initials: EC
authors.lastname: Gartuzo
authors.forename: Ivan
authors.initials: I
authors.lastname: Pagotto
authors.forename: Edson J
authors.initials: EJ
authors.lastname: Comparetti
authors.forename: Salomón
authors.initials: S
authors.lastname: Huancahuire-Vega
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Ponce-Soto
authors.forename: Tássia R
authors.initials: TR
authors.lastname: Costa
authors.forename: Sergio
authors.initials: S
authors.lastname: Marangoni
authors.forename: Andreimar M
authors.initials: AM
authors.lastname: Soares
authors.forename: Marcos R M
authors.initials: MR
authors.lastname: Fontes
mesh_terms: D000818:Animals; D017837:Bothrops; D003435:Crotalid Venoms / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D007527:Isoenzymes / Q000737:chemistry; D054467:Phospholipases A2 / Q000737:chemistry*
chemical_list: D003435:Crotalid Venoms; D007527:Isoenzymes; D054467:Phospholipases A2