title: Inhibition of micturition reflex by activation of somatic afferents in posterior femoral cutaneous nerve.
abstract: This study determined if activation of somatic afferents in posterior femoral cutaneous nerve (PFCN) could modulate the micturition reflex recorded under isovolumetric conditions in α-chloralose anaesthetized cats. PFCN stimulation inhibited reflex bladder activity and significantly (P <0.05) increased bladder capacity during slow infusion of saline or 0.25% acetic acid (AA). The optimal frequency for PFCN stimulation-induced bladder inhibition was between 3 and 10 Hz, and a minimal stimulation intensity of half of the threshold for inducing anal twitching was required. Bilateral pudendal nerve transection eliminated PFCN stimulation-induced anal twitching but did not change the stimulation-induced bladder inhibition, excluding the involvement of pudendal afferent or efferent axons in PFCN afferent inhibition.Mechanical or electrical stimulation on the skin surface in the PFCN dermatome also inhibited bladder activity. Prolonged (2 × 30 min) PFCN stimulation induced a post-stimulation inhibition that persists for at least 2 h. This study revealed a new cutaneous-bladder reflex activated by PFCN afferents. Although the mechanisms and physiological functions of this cutaneous-bladder reflex need to be further studied, our data raise the possibility that stimulation of PFCN afferents might be useful clinically for the treatment of overactive bladder symptoms.
authors.affiliation: Department of Urology, University of Pittsburgh, 700 Kaufmann Building, Pittsburgh, PA 15213, USA. cftai@pitt.edu
authors.forename: Changfeng
authors.initials: C
authors.lastname: Tai
authors.forename: Bing
authors.initials: B
authors.lastname: Shen
authors.forename: Abhijith D
authors.initials: AD
authors.lastname: Mally
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Shouguo
authors.initials: S
authors.lastname: Zhao
authors.forename: Jicheng
authors.initials: J
authors.lastname: Wang
authors.forename: James R
authors.initials: JR
authors.lastname: Roppolo
authors.forename: William C
authors.initials: WC
authors.lastname: de Groat
mesh_terms: D000818:Animals; D002415:Cats; D005260:Female; D008297:Male; D060525:Pudendal Nerve / Q000502:physiology*; D012018:Reflex / Q000502:physiology*; D012867:Skin / Q000294:innervation*; D004561:Transcutaneous Electric Nerve Stimulation; D001743:Urinary Bladder / Q000294:innervation / Q000502:physiology*; D014554:Urination / Q000502:physiology*