title: Application of a novel multiplex polymerase chain reaction system for 12 X-chromosomal short tandem repeats to a Japanese population study.
abstract: The analysis of X-chromosomal short tandem repeat (X-STR) polymorphisms has been the focus of attention in several researches, mainly due to its applicability in the investigation of complex kinship cases. A new 12 X-STR multiplex system (GATA172D05, DXS7423, DSX6809, DXS10134, DXS7132, DXS9902, DXS6789, DXS10074, DXS8378, DXS9898, DXS10147, and GATA31E08) was developed and applied to a Japanese population study. DNA samples from 290 males and 160 females were successfully analyzed using the 12 X-STR multiplex system. No mutation was detected in the kinship cases involving 34 family trios. The combined powers of discrimination of the 12 X-STR loci in males and females were 0.999997 and 0.9999999996, respectively. We conclude that the combined analysis of 12 X-STR loci using this single multiplex polymerase chain reaction system is a powerful tool in forensic DNA testing.
authors.affiliation: Department of Legal Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Ten-nohdai, Tsukuba, Japan. s1130407@u.tsukuba.ac.jp
authors.forename: Takeki
authors.initials: T
authors.lastname: Nishi
authors.forename: Akira
authors.initials: A
authors.lastname: Kurosu
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sugano
authors.forename: Junko
authors.initials: J
authors.lastname: Kaminiwa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sekine
authors.forename: Shizue
authors.initials: S
authors.lastname: Yano
authors.forename: Katsuya
authors.initials: K
authors.lastname: Honda
mesh_terms: D041321:Chromosomes, Human, X / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D005260:Female; D053803:Forensic Genetics / Q000379:methods* / Q000639:trends; D005787:Gene Frequency; D008040:Genetic Linkage; D006801:Humans; D007564:Japan; D008297:Male; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction
chemical_list: D017931:DNA Primers