title: Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae infection in a neonatal intensive care unit.
abstract: BACKGROUND
  A molecular epidemiological survey was conducted on an extended-spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBLKp) infection in our neonatal intensive care unit (NICU) from February to June 2008.
  
  
  METHODS
  Cultures of clinical samples from neonates in the NICU, the hands of healthcare workers and the environment of the NICU were subjected to ESBLKp isolation. Pulsed-field gel electrophoresis was performed to determine Klebsiella pneumoniae strains (type A-D).
  
  
  RESULTS
  In 1439 neonates, 38 (2.6%) had infections and 65 (4.5%) had colonizations with ESBLKp. Microbiological sampling of the NICU environment yielded 33 (14.9%) ESBLKp isolates from 222 samples. Clone A was found in 88.2% of the infected neonates, 66.7% of the colonized neonates, 69.7% of the environmental samples, and the hands of a healthcare worker.
  
  
  CONCLUSIONS
  The detection rate of ESBLKp is high in environmental samples, especially those from frequently touched surfaces. Since ESBLKp was identified on the hands of a healthcare worker in the present study, hand and environmental hygiene is mandatory for infection control in neonatal intensive care units.
authors.affiliation: Department of Infection Control, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Lin
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Xin-Fen
authors.initials: XF
authors.lastname: Xu
authors.forename: Xin-Chang
authors.initials: XC
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Ye
authors.forename: Guang-Yong
authors.initials: GY
authors.lastname: Ye
mesh_terms: D002681:China / Q000453:epidemiology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission*; D004196:Disease Outbreaks / Q000706:statistics & numerical data*; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000201:enzymology*; D008297:Male; D017720:Molecular Epidemiology; D058889:Molecular Typing; D012307:Risk Factors; D001618:beta-Lactamases
chemical_list: D001618:beta-Lactamases