title: [Therapeutic effects of unfractionated heparin on lipopolysaccharide-activated matrix metalloproteinase-9 and tissue inhibitors of metalloproteinase-1 in endothelial cells].
abstract: OBJECTIVE
  To investigate the expressions of matrix metalloproteinase-9 and tissue inhibitors of metalloproteinase-1 (TIMP-1) in injured endothelial cells induced by lipopolysaccharide (LPS) and the effects of unfractionated heparin (UFH) on the level of expressions.
  
  
  METHODS
  The human pulmonary microvascular endothelial cells (HPMECs) were injured by LPS (10 μg/ml). In UFH pretreatment group, the cells were interfered with 0.1 U/ml or 1 U/ml UFH within 15 minutes before stimulus of LPS. In control group, the cells were cultured in equal volume of phosphate buffered saline (PBS). The RNA of the respectively cells were extracted at 2, 6, 12 hours after stimulus, and the expressions of MMP-9 mRNA and TIMP-1 mRNA were detected by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  Compared with control group, the expressions of MMP-9 mRNA and TIMP-1 mRNA were increased after stimulation of LPS, and peaked at 12 hours (MMP-9 mRNA: 4.26±0.81 vs. 1.00±0.46, TIMP-1 mRNA: 4.93±0.08 vs. 1.00±0.13, both P<0.05), the change in TIMP-1 was more significant. While as UFH pretreatment could significantly down-regulated the mRNA expressions of MMP-9 and TIMP-1 (UFH 0.1 U/ml group MMP-9 mRNA: 2.74±0.30, TIMP-1 mRNA: 2.96±0.13; UFH 1 U/ml group MMP-9 mRNA: 3.08±0.48, TIMP-1 mRNA: 2.93±0.27, all P<0.05). There were no significant differences in mRNA expressions between two UFH groups.
  
  
  CONCLUSIONS
  The expressions of MMP-9 and TIMP-1 of HPMEC injured by LPS were obviously increased, UFH might attenuate the injury via inhibiting the expressions of MMP-9 and TIMP-1.
authors.affiliation: First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Xiao-chun
authors.initials: XC
authors.lastname: Ma
mesh_terms: D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000009:adverse effects; D008168:Lung / Q000098:blood supply; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D006493:Heparin; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9