title: Microplate well coverage mixing using superhydrophobic contact.
abstract: Two important challenges in microplate instrumentation are to achieve full well sample coverage and complete mixing. An effective approach of using superhydrophobic rods to accomplish these challenges is reported here. Experiments conducted showed that analytes above 50μl could be made to completely cover the bottom of 96-well standard and transparency microplates. Complete mixing was accomplished by moving the rod parallel to the well bottom while contacting the liquid. The approach is simple and controlled, and it minimizes the problems of spillage and cross-contamination. It works with analytes with varied volumes and of different viscosities present in each well of the microplate.
authors.affiliation: Laboratory for Optics, Acoustics, and Mechanics, Department of Mechanical and Aerospace Engineering, Monash University, Clayton, Victoria, Australia.
authors.forename: Thach
authors.initials: T
authors.lastname: Vuong
authors.forename: Brandon Huey-Ping
authors.initials: BH
authors.lastname: Cheong
authors.forename: Jonathan Kok Keung
authors.initials: JK
authors.lastname: Lye
authors.forename: Oi Wah
authors.initials: OW
authors.lastname: Liew
authors.forename: Tuck Wah
authors.initials: TW
authors.lastname: Ng
mesh_terms: D057927:Hydrophobic and Hydrophilic Interactions; D055616:Microtechnology / Q000295:instrumentation*; D017155:Wettability