title: H-point curve isolation method for determination of catechol in complex unknown mixtures.
abstract: In this work, the combination of H-point curve isolation method (HPCIM) and H-point standard additions method (HPSAM) was used for determination of catechol in the presence of phenolic interferents. Spectrophotometric multivariate calibration data constructed by successive standard additions of an analyte in an unknown matrix was used by the method. A cumulative spectrum for interferents in sample was extracted by HPCIM and then HPSAM is used for determination of the catechol concentration by obtained cumulative interferents spectrum. The method was tested with simulated data set. The spectrum obtained from applying HPCIM to the simulated data well agrees with the cumulative spectra of the interferents. The method was applied to the determination of catechol in the presence of highly overlapping interferents in synthetic ternary mixtures using spectrophotometric data. Moreover, the proposed method was successfully used for determination of catechol in real complicated matrices of tea and urine samples. Percent recoveries were between 95.4 and 113.6.
authors.affiliation: Faculty of Chemistry, Bu-Ali Sina University, Hamedan 65174, Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Hasani
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Mohammadi
authors.forename: Masoud
authors.initials: M
authors.lastname: Shariati-Rad
authors.forename: Hamid
authors.initials: H
authors.lastname: Abdollahi
mesh_terms: D000042:Absorption; D002396:Catechols / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D045424:Complex Mixtures / Q000737:chemistry*; D003198:Computer Simulation; D006863:Hydrogen-Ion Concentration; D006873:Hydroquinones / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D012118:Resorcinols / Q000737:chemistry; D013662:Tea / Q000737:chemistry
chemical_list: D002396:Catechols; D045424:Complex Mixtures; D006873:Hydroquinones; D010636:Phenols; D012118:Resorcinols; D013662:Tea; C018599:4-methylcatechol; C034221:catechol; C031927:hydroquinone; C031389:resorcinol