title: Amperometric microbiosensor as an alternative tool for investigation of D-serine in brain.
abstract: This paper discusses the application of a reagentless, selective microbiosensor as a useful alternative tool for monitoring D-serine in neural samples. The main components of the 125-μm-diameter disk biosensor were D-amino acid oxidase for D-serine sensitivity (linear region slope, 61 ± 7 μA cm(-2) mM(-1); limit of detection, 20 nM), and poly-phenylenediamine for rejection of electroactive interference. The response time of the biosensor was of the order of 1 s, ideal for 'real-time' monitoring, and detection of systemically administered D-serine in brain extracellular fluid is demonstrated. Exploitation of this probe might resolve queries involving regulation of D-serine in excitotoxicity, and modulation of N-methyl-D-aspartate receptor function by D-serine and glycine in the central nervous system.
authors.affiliation: Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia. zainihar@salam.uitm.edu.my
authors.forename: Zainiharyati
authors.initials: Z
authors.lastname: Mohd Zain
authors.forename: Sulaiman
authors.initials: S
authors.lastname: Ab Ghani
authors.forename: Robert D
authors.initials: RD
authors.lastname: O'Neill
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D001923:Brain Chemistry / Q000187:drug effects*; D003605:D-Amino-Acid Oxidase / Q000737:chemistry / Q000378:metabolism; D004567:Electrodes, Implanted; D045604:Extracellular Fluid / Q000737:chemistry / Q000187:drug effects*; D005998:Glycine / Q000494:pharmacology; D007700:Kinetics; D057230:Limit of Detection; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010655:Phenylenediamines / Q000737:chemistry; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D012694:Serine / Q000378:metabolism* / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: D010655:Phenylenediamines; D016194:Receptors, N-Methyl-D-Aspartate; C530487:poly(o-phenylenediamine); D012694:Serine; D003605:D-Amino-Acid Oxidase; D005998:Glycine