title: CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.
abstract: Innate immune cells, such as intestinal epithelial cells, dendritic cells (DCs), macrophages, granulocytes, and innate lymphoid cells provide a first line of defence to enteric pathogens. To study the role of CX(3)CR1(+) DCs and macrophages in host defence, we infected CX(3)CR1-GFP animals with Citrobacter rodentium. When transgenic CX(3)CR1-GFP animals are infected with the natural mouse pathogen C. rodentium, CX(3)CR1(-/-) animals showed a delayed clearance of C. rodentium as compared with (age- and sex-matched) wild-type B6 animals. The delayed clearance of C. rodentium is associated with reduced interleukin (IL)-22 expression. In C. rodentium-infected CX(3)CR1-GFP animals, IL-22 producing lymphoid-tissue inducer cells (LTi cells) were selectively reduced in the absence of CX(3)CR1. The reduced IL-22 expression correlates with decreased expression of the antimicrobial peptides RegIIIβ and RegIIIγ. The depletion of CX(3)CR1(+) cells by diphtheria toxin injection in CX(3)CR1-GFP × CD11c.DOG animals confirmed the role of CX(3)CR1(+) phagocytes in establishing IL-22 production, supporting the clearance of a C. rodentium infection.
authors.affiliation: Department of Internal Medicine I, University of Ulm, Ulm, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Manta
authors.forename: E
authors.initials: E
authors.lastname: Heupel
authors.forename: K
authors.initials: K
authors.lastname: Radulovic
authors.forename: V
authors.initials: V
authors.lastname: Rossini
authors.forename: N
authors.initials: N
authors.lastname: Garbi
authors.forename: C U
authors.initials: CU
authors.lastname: Riedel
authors.forename: J H
authors.initials: JH
authors.lastname: Niess
mesh_terms: D000818:Animals; D039521:CD11c Antigen / Q000378:metabolism; D000073883:CX3C Chemokine Receptor 1; D044082:Citrobacter rodentium / Q000276:immunology*; D004195:Disease Models, Animal; D004756:Enterobacteriaceae Infections / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010586:Phagocytes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*; D000097043:Interleukin-22
chemical_list: D039521:CD11c Antigen; D000073883:CX3C Chemokine Receptor 1; D007378:Interleukins; D019707:Receptors, Chemokine; D000097043:Interleukin-22; C471885:Cx3cr1 protein, mouse