title: Cardioprotective effect of ischemic postconditioning on the model of isolated heart.
abstract: Irreversible cardiomyocyte damage was induced by 45-min global ischemia followed by 30-min reperfusion in Langendorff-perfused isolated rat heart. Cell damage was assessed by the release of creatine phosphokinase into the perfusate. The hearts were subjected to the following postconditioning protocols: 1) three cycles of 10-sec reperfusion and 10-sec ischemia, total cycle time 20 sec; 2) six cycles of 10-sec reperfusion and 10-sec ischemia, total cycle time 20 sec; 3) three sessions of 20-sec reperfusion and 20-sec ischemia, total cycle time 40 sec; 4) 6 cycles of 20-sec reperfusion and 20-sec ischemia, total cycle time 40 sec; 5) 3 cycles of 30-sec reperfusion and 30-sec ischemia, total cycle time 60 sec. It was found that only postconditioning with a total cycle time of 40 sec or 60 sec prevents myocardial reperfusion injury.
authors.affiliation: Institute of Cardiology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, Russia. Maslov@cardio.tsu.ru
authors.forename: L N
authors.initials: LN
authors.lastname: Maslov
authors.forename: A S
authors.initials: AS
authors.lastname: Gorbunov
authors.forename: Yu B
authors.initials: YB
authors.lastname: Lishmanov
mesh_terms: D000818:Animals; D006321:Heart / Q000503:physiopathology*; D066298:In Vitro Techniques; D057775:Ischemic Postconditioning / Q000379:methods*; D019157:Ischemic Preconditioning, Myocardial; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar