title: Trapping DNA near a solid-state nanopore.
abstract: We demonstrate that voltage-biased solid-state nanopores can transiently localize DNA in an electrolyte solution. A double-stranded DNA (dsDNA) molecule is trapped when the electric field near the nanopore attracts and immobilizes a non-end segment of the molecule across the nanopore orifice without inducing a folded molecule translocation. In this demonstration of the phenomenon, the ionic current through the nanopore decreases when the dsDNA molecule is trapped by the nanopore. By contrast, a translocating dsDNA molecule under the same conditions causes an ionic current increase. We also present finite-element modeling results that predict this behavior for the conditions of the experiment.
authors.affiliation: Department of Physics, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: Dimitar M
authors.initials: DM
authors.lastname: Vlassarev
authors.forename: Jene A
authors.initials: JA
authors.lastname: Golovchenko
mesh_terms: D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D004560:Electricity; D004573:Electrolytes / Q000737:chemistry; D020342:Finite Element Analysis; D058608:Nanopores / Q000648:ultrastructure; D012996:Solutions; D013499:Surface Properties
chemical_list: D004573:Electrolytes; D012996:Solutions; D004247:DNA