title: RCY1-mediated resistance to Cucumber mosaic virus is regulated by LRR domain-mediated interaction with CMV(Y) following degradation of RCY1.
abstract: RCY1, which encodes a coiled coil nucleotide-binding site leucine-rich repeat (LRR) class R protein, confers the hypersensitive response (HR) to a yellow strain of Cucumber mosaic virus (CMV[Y]) in Arabidopsis thaliana. Nicotiana benthamiana transformed with hemagglutinin (HA) epitope-tagged RCY1 (RCY1-HA) also exhibited a defense response accompanied by HR cell death and induction of defense-related gene expression in response to CMV(Y). Following transient expression of RCY1-HA by agroinfiltration, the defense reaction was induced in N. benthamiana leaves infected with CMV(Y) but not in virulent CMV(B2)-infected N. benthamiana leaves transiently expressing RCY1-HA or CMV(Y)-infected N. benthamiana leaves transiently expressing HA-tagged RPP8 (RPP8-HA), which is allelic to RCY1. This result suggests that Arabidopsis RCY1-conferred resistance to CMV(Y) could be reproduced in N. benthamiana leaves in a gene-for-gene manner. Expression of a series of chimeric constructs between RCY1-HA and RPP8-HA in CMV(Y)-infected N. benthamiana indicated that induction of defense responses to CMV(Y) is regulated by the LRR domain of RCY1. Interestingly, in CMV(Y)-infected N. benthamiana manifesting the defense response, the levels of both RCY1 and chimeric proteins harboring the RCY1 LRR domain were significantly reduced. Taken together, these data indicate that the RCY1-conferred resistance response to CMV(Y) is regulated by an LRR domain-mediated interaction with CMV(Y) and seems to be tightly associated with the degradation of RCY1 in response to CMV(Y).
authors.affiliation: Department of Life Science, Tohoku University, Sendai, Japan. takahash@bios.tohoku.ac.jp
authors.forename: Hideki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Shoji
authors.forename: Sugihiro
authors.initials: S
authors.lastname: Ando
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kanayama
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Kusano
authors.forename: Minoru
authors.initials: M
authors.lastname: Takeshita
authors.forename: Masashi
authors.initials: M
authors.lastname: Suzuki
authors.forename: Chikara
authors.initials: C
authors.lastname: Masuta
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D016923:Cell Death; D017799:Cucumovirus / Q000276:immunology*; D018506:Gene Expression Regulation, Plant / Q000276:immunology; D010935:Plant Diseases / Q000276:immunology / Q000821:virology*; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism / Q000821:virology; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary / Q000502:physiology; D059748:Proteolysis; D000068103:Serine-Arginine Splicing Factors; D014026:Nicotiana / Q000235:genetics* / Q000276:immunology / Q000821:virology; D014170:Transformation, Genetic
chemical_list: D029681:Arabidopsis Proteins; C473790:RCY1 protein, Arabidopsis; D000068103:Serine-Arginine Splicing Factors