title: "Low-grade positivity" of HPV viral load after atypical squamous cells of undetermined significance (ASC-US) cytology identifies women at low-risk for cervical intraepithelial neoplasia grade 2 and 3.
abstract: The correlation between high-risk HPV-DNA viral load, expressed as relative light units (RLU) values obtained from the Hybrid Capture 2 (HC2) test, and the prevalence of CIN2/CIN3 was investigated and statistically analyzed in 614 ASC-US consecutive cases. Cases were categorized into three groups according to RLU values: "low-grade positivity", "intermediate positivity" and "high-grade positivity", and the prevalence of CIN2/CIN3 was evaluated in the single groups and compared among them. CIN/CIN3 rates demonstrated a significant (p < 0.001) increase with a direct correlation with increasing RLU values: 4.6% (RLU from 1.0 to 10.0), 9.1% (RLU from 11.0 to 100.0) and 32.2% (RLU > 100.0) respectively. The prevalence of CIN2/CIN3 between the group with RLU < 10.0 (4.6%) and the group with RLU > 10 (24.2%) showed statistical significance (p = 0.0002). Increasing hrHPV viral load significantly correlates with increasing prevalence of CIN2/CIN3 in ASC-US cases.
authors.affiliation: Department of Gynecology & Obstetrics, Vita Salute San Raffaele University School of Medicine at San Raffaele Scientific Institute, Milano, Italy. massimo.origoni@hsr.it
authors.forename: M
authors.initials: M
authors.lastname: Origoni
authors.forename: G
authors.initials: G
authors.lastname: Carminati
authors.forename: M
authors.initials: M
authors.lastname: Sideri
authors.forename: M
authors.initials: M
authors.lastname: Clementi
authors.forename: S
authors.initials: S
authors.lastname: Rolla
authors.forename: M
authors.initials: M
authors.lastname: Candiani
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D060787:Neoplasm Grading; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000821:virology*; D012189:Retrospective Studies; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000821:virology; D019562:Viral Load; D002578:Uterine Cervical Dysplasia / Q000473:pathology* / Q000821:virology
chemical_list: D004279:DNA, Viral