title: BODIPY-based molecular probe for imaging of cerebral β-amyloid plaques.
abstract: We designed and synthesized a BODIPY-based probe (BAP-1) for the imaging of β-amyloid plaques in the brain. In binding experiments in vitro, BAP-1 showed excellent affinity for synthetic Aβ aggregates. β-Amyloid plaques in Tg2576 mouse brain were clearly visualized with BAP-1. In addition, the labeling of β-amyloid plaques was demonstrated in vivo in Tg2576 mice. These results suggest BAP-1 to be a useful fluorescent probe for the optical imaging of cerebral β-amyloid plaques in patients with Alzheimer's disease.
authors.affiliation: Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University , 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan. ono@pharm.kyoto-u.ac.jp
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ono
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Watanabe
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kimura
authors.forename: Hideo
authors.initials: H
authors.lastname: Saji
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000502:physiology; D000818:Animals; D001896:Boron Compounds / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry / Q000502:physiology; D003952:Diagnostic Imaging / Q000379:methods; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D015335:Molecular Probes / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods; D058225:Plaque, Amyloid / Q000737:chemistry* / Q000378:metabolism
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D016229:Amyloid beta-Peptides; D001896:Boron Compounds; D005456:Fluorescent Dyes; D015335:Molecular Probes