title: Characterization and fine mapping of a novel rice albino mutant low temperature albino 1.
abstract: Albino mutants are useful genetic resource for studying chlorophyll biosynthesis and chloroplast development and cloning genes involved in these processes in plants. Here we report a novel rice mutant low temperature albino 1 (lta1) that showed albino leaves before 4-leaf stage when grown under temperature lower than 20°C, but developed normal green leaves under temperature higher than 24°C or similar morphological phenotypes in dark as did the wild-type (WT). Our analysis showed that the contents of chlorophylls and chlorophyll precursors were remarkably decreased in the lta1 mutant under low temperature compared to WT. Transmission electron microscope observation revealed that chloroplasts were defectively developed in the albino lta1 leaves, which lacked of well-stacked granum and contained less stroma lamellae. These results suggested that the lta1 mutation may delay the light-induced thylakoid assembly under low temperature. Genetic analysis indicated that the albino phenotype was controlled by a single recessive locus. Through map-based approach, we finally located the Lta1 gene to a region of 40.3 kb on the short arm of chromosome 11. There are 8 predicted open reading frames (ORFs) in this region and two of them were deleted in lta1 genome compared with the WT genome. The further characterization of the Lta1 gene would provide a good approach to uncover the novel molecular mechanisms involved in chloroplast development under low temperature stress.
authors.affiliation: Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Peng
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Lv
authors.forename: Jianhui
authors.initials: J
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Shi
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Honglei
authors.initials: H
authors.lastname: Zhang
authors.forename: Zuhua
authors.initials: Z
authors.lastname: He
authors.forename: Sheng
authors.initials: S
authors.lastname: Teng
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003080:Cold Temperature; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics
chemical_list: D010940:Plant Proteins