title: Solution NMR and X-ray crystal structures of Pseudomonas syringae Pspto_3016 from protein domain family PF04237 (DUF419) adopt a "double wing" DNA binding motif.
abstract: The protein Pspto_3016 is a 117-residue member of the protein domain family PF04237 (DUF419), which is to date a functionally uncharacterized family of proteins. In this report, we describe the structure of Pspto_3016 from Pseudomonas syringae solved by both solution NMR and X-ray crystallography at 2.5 Å resolution. In both cases, the structure of Pspto_3016 adopts a "double wing" α/β sandwich fold similar to that of protein YjbR from Escherichia coli and to the C-terminal DNA binding domain of the MotA transcription factor (MotCF) from T4 bacteriophage, along with other uncharacterized proteins. Pspto_3016 was selected by the Protein Structure Initiative of the National Institutes of Health and the Northeast Structural Genomics Consortium (NESG ID PsR293).
authors.affiliation: Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.
authors.forename: Erik A
authors.initials: EA
authors.lastname: Feldmann
authors.forename: Jayaraman
authors.initials: J
authors.lastname: Seetharaman
authors.forename: Theresa A
authors.initials: TA
authors.lastname: Ramelot
authors.forename: Scott
authors.initials: S
authors.lastname: Lew
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Keith
authors.initials: K
authors.lastname: Hamilton
authors.forename: Colleen
authors.initials: C
authors.lastname: Ciccosanti
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Acton
authors.forename: John K
authors.initials: JK
authors.lastname: Everett
authors.forename: Liang
authors.initials: L
authors.lastname: Tong
authors.forename: Gaetano T
authors.initials: GT
authors.lastname: Montelione
authors.forename: Michael A
authors.initials: MA
authors.lastname: Kennedy
mesh_terms: D020816:Amino Acid Motifs; D001426:Bacterial Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008957:Models, Genetic; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D040901:Proteomics / Q000379:methods*; D044224:Pseudomonas syringae / Q000737:chemistry* / Q000235:genetics; D012996:Solutions / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; D012996:Solutions