title: High-throughput karyotyping of human pluripotent stem cells.
abstract: Genomic integrity of human pluripotent stem cell (hPSC) lines requires routine monitoring. We report here that novel karyotyping assay, utilizing bead-bound bacterial artificial chromosome probes, provides a fast and easy tool for detection of chromosomal abnormalities in hPSC lines. The analysis can be performed from low amounts of DNA isolated from whole cell pools with simple data analysis interface. The method enables routine screening of stem cell lines in a cost-efficient high-throughput manner.
authors.affiliation: Molecular and Systems Immunology and Stem Cell Biology, Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, FI-20520 Turku, Finland. riikka.lund@btk.fi
authors.forename: Riikka J
authors.initials: RJ
authors.lastname: Lund
authors.forename: Tuomas
authors.initials: T
authors.lastname: Nikula
authors.forename: Nelly
authors.initials: N
authors.lastname: Rahkonen
authors.forename: Elisa
authors.initials: E
authors.lastname: Närvä
authors.forename: Duncan
authors.initials: D
authors.lastname: Baker
authors.forename: Neil
authors.initials: N
authors.lastname: Harrison
authors.forename: Peter
authors.initials: P
authors.lastname: Andrews
authors.forename: Timo
authors.initials: T
authors.lastname: Otonkoski
authors.forename: Riitta
authors.initials: R
authors.lastname: Lahesmaa
mesh_terms: D002460:Cell Line; D002875:Chromosomes / Q000235:genetics; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D039904:Pluripotent Stem Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism