title: Mesenchymal stem cell interactions with 3D ECM modules fabricated via multiphoton excited photochemistry.
abstract: To understand complex micro/nanoscale ECM stem cell interactions, reproducible in vitro models are needed that can strictly recapitulate the relative content and spatial arrangement of native tissue. Additionally, whole ECM proteins are required to most accurately reflect native binding dynamics. To address this need, we use multiphoton excited photochemistry to create 3D whole protein constructs or "modules" to study how the ECM governs stem cell migration. The constructs were created from mixtures of BSA/laminin (LN) and BSA alone, whose comparison afforded studying how the migration dynamics are governed from the combination of morphological and ECM cues. We found that mesenchymal stem cells interacted for significantly longer durations with the BSA/LN constructs than pure BSA, pointing to the importance of binding cues of the LN. Critical to this work was the development of an automated system with feedback based on fluorescence imaging to provide quality control when synthesizing multiple identical constructs.
authors.affiliation: Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Ping-Jung
authors.initials: PJ
authors.lastname: Su
authors.forename: Quyen A
authors.initials: QA
authors.lastname: Tran
authors.forename: Jimmy J
authors.initials: JJ
authors.lastname: Fong
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Eliceiri
authors.forename: Brenda M
authors.initials: BM
authors.lastname: Ogle
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Campagnola
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D005456:Fluorescent Dyes; D006801:Humans; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D061848:Optical Imaging; D010777:Photochemistry / Q000379:methods*; D011485:Protein Binding; D012395:Rose Bengal; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D059008:Time-Lapse Imaging; D014966:Xanthenes
chemical_list: D016326:Extracellular Matrix Proteins; D005456:Fluorescent Dyes; D007797:Laminin; D014966:Xanthenes; D012395:Rose Bengal; D012710:Serum Albumin, Bovine; C034657:Texas red