title: Characterization of multiple absorbed constituents in rats after oral administration of Paederia scandens decoction.
abstract: Paederia scandens (Lour.) Merri. (Jishiteng in Chinese) is a Chinese traditional medicine widely used in treating various diseases. However, its active components have remained unknown. In the present study, a rapid and sensitive method by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-MSn) techniques was employed to investigate the absorbed constituents in rats after oral administration of Paederia scandens decoction. By comparing their MS data with those of authentic compounds and published data, a total of six compounds (paederosid, 1; paederosidic acid, 2; paederosidic acid methyl ester, 3; 6-hydroxy geniposide, 4; asperuloside, 5; and deacetyl asperuloside, 6) were identified in the P. scandens decoction samples. In addition, a total of seven compounds, including three iridoid glucosides and four of their metabolites, were identified in rat urine samples after administration. In addition, six compounds, including four iridoid glucosides and two of their metabolites, were identified in rat serum samples after administration. Our results significantly narrow the range of potentially active compounds in P. scandens decoction, and build a solid foundation for future research on its mechanism.
authors.affiliation: Harbin University of Commerce, Harbin 150076, People's Republic of China.
authors.forename: Weixin
authors.initials: W
authors.lastname: Jiang
authors.forename: Di
authors.initials: D
authors.lastname: Jin
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Li
authors.forename: Zhaolin
authors.initials: Z
authors.lastname: Sun
authors.forename: Mingcang
authors.initials: M
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Chenggang
authors.initials: C
authors.lastname: Huang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics*; D057888:Iridoid Glucosides / Q000008:administration & dosage / Q000097:blood / Q000652:urine; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D019666:Rubiaceae / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D057888:Iridoid Glucosides