title: Molecular characterization of a new lytic bacteriophage isolated from cheese whey.
abstract: In this study, we isolated and characterized a lytic Lactococcus lactis bacteriophage from the sera of a failed fermentation. The phage was isolated and cultured in L. lactis subsp. cremoris in M17 medium. The isolated bacteriophage was characterized by multiplex PCR, pulsed-field electrophoresis, DNA restriction digestion, analysis of the N-terminal sequence of the phage major structural protein, transmission electron microscopy and sequencing and analysis of a conserved fragment of its genome. Analysis of the viral genome indicates that its genome is composed of a DNA strand of approximately 48 kb in length, and PCR and microscopy confirmed that IL-P1 belongs to the group of 936-type phages in the family Siphoviridae, which is the most abundant type of lactococcal virus in dairy products worldwide. To our knowledge, this is the first report of a virus within this family that has a presumptive genome larger than 40 kb.
authors.affiliation: Laboratory of Molecular Immunovirology, Department of General Biology, Federal University of Viçosa, Viçosa, Brazil.
authors.forename: M R
authors.initials: MR
authors.lastname: Eller
authors.forename: R S
authors.initials: RS
authors.lastname: Dias
authors.forename: C A
authors.initials: CA
authors.lastname: De Moraes
authors.forename: A F
authors.initials: AF
authors.lastname: De Carvalho
authors.forename: L L
authors.initials: LL
authors.lastname: Oliveira
authors.forename: E A M
authors.initials: EA
authors.lastname: Silva
authors.forename: C C
authors.initials: CC
authors.lastname: da Silva
authors.forename: S O
authors.initials: SO
authors.lastname: De Paula
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002611:Cheese / Q000382:microbiology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D016679:Genome, Viral / Q000235:genetics; D013294:Lactococcus lactis / Q000821:virology*; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016415:Sequence Alignment; D017906:Siphoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins