title: Sensitive determination of proteins by its quenching effect on fluorescence of new terbium(III) complex.
abstract: It is found that in hexamethylenetetramine (HMTA-HCl) buffer pH = 7.8, proteins can quench the fluorescence intensity of new terbium(III) complex with 6-[(1-hydroxy-3-oxo-6,7-dihydro-3H,5H-pyrido[3,2,1-ij]quinoline-2-carbonyl)-amino]-hexanoic acid (L). Based on this, a sensitive fluorimetric method for the determination of proteins is proposed. Under optimum conditions, the I0/I is in proportion to the concentration of protein in the range of 0.1-40.0 microg/mL for bovine serum albumin (BSA), 0.1-70.0 microg/mL for human serum albumin (HSA) and 0.1-40.0 microg/mL for immunoglobulin G (IgG). Their detection limits (S/N = 3) are 0.03 microg/mL. The interaction mechanism for the luminescence quenching is also studied.
authors.affiliation: A. V. Bogatsky Physico-Chemical Institute of National Academy of Sciences of Ukraine, Odessa 65080, Ukraine.
authors.forename: Inna
authors.initials: I
authors.lastname: Leonenko
authors.forename: Daria
authors.initials: D
authors.lastname: Aleksandrova
authors.forename: Alla
authors.initials: A
authors.lastname: Yegorova
authors.forename: Valery
authors.initials: V
authors.lastname: Antonovich
authors.forename: Aleksandr
authors.initials: A
authors.lastname: Karasyov
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D002138:Calibration; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007136:Immunoglobulins / Q000032:analysis / Q000097:blood; D057230:Limit of Detection; D012015:Reference Standards; D012709:Serum Albumin / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods* / Q000592:standards; D013056:Spectrophotometry, Ultraviolet; D013725:Terbium / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D005456:Fluorescent Dyes; D007136:Immunoglobulins; D012709:Serum Albumin; D013725:Terbium; D012710:Serum Albumin, Bovine