title: Myelination and oxidative stress alterations in the cerebellum of the G72/G30 transgenic schizophrenia mouse model.
abstract: G72/G30 is a primate-specific locus that has been repeatedly implicated as a risk factor in genetic studies of schizophrenia. The function of the longest G72 splice variant (LG72 protein) encoded by this locus is not fully understood. To investigate the role of the LG72 protein in vivo, we have generated transgenic (G72Tg) mice carrying the G72/G30 locus that exhibit schizophrenia-like symptoms. We investigated protein expression alterations in the cerebella of G72Tg compared to wild type (WT) mice using a proteomics approach based on in vivo(15)N metabolic labeling and quantitative mass spectrometry (MS). Our data revealed expression level differences of proteins involved in myelin-related processes, oxidative stress and mitochondrial function. Furthermore, in silico pathway analyses suggested common regulators and targets for the observed protein alterations. Our work sheds light on the functional role of the LG72 protein and pinpoints molecular correlates of schizophrenia-like behavior.
authors.affiliation: Proteomics and Biomarkers, Max Planck Institute of Psychiatry, Kraepelinstr 2-10, D-80804 Munich, Germany. mfiliou@mpipsykl.mpg.de
authors.forename: Michaela D
authors.initials: MD
authors.lastname: Filiou
authors.forename: Larysa
authors.initials: L
authors.lastname: Teplytska
authors.forename: David M
authors.initials: DM
authors.lastname: Otte
authors.forename: Andreas
authors.initials: A
authors.lastname: Zimmer
authors.forename: Christoph W
authors.initials: CW
authors.lastname: Turck
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D002374:Catalase / Q000378:metabolism; D002531:Cerebellum / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D009413:Nerve Fibers, Myelinated / Q000473:pathology*; D009587:Nitrogen Isotopes; D018384:Oxidative Stress / Q000235:genetics*; D040901:Proteomics; D015203:Reproducibility of Results; D012559:Schizophrenia / Q000235:genetics* / Q000473:pathology*
chemical_list: D002352:Carrier Proteins; C475071:DAOA protein, human; D047908:Intracellular Signaling Peptides and Proteins; D009587:Nitrogen Isotopes; D002374:Catalase