title: Anion complexation and transport by isophthalamide and dipicolinamide derivatives: DNA plasmid transformation in E. coli.
abstract: Tris-arenes based on either isophthalic acid or 2,6-dipicolinic acid have been known for more than a decade to bind anions. Recent studies have also demonstrated their ability to transport various ions through membranes. In this report, we demonstrate two important properties of these simple diamides. First, they transport plasmid DNA into Escherichia coli about 2-fold over controls, where the ampicillin resistance gene is expressed in the bacteria. These studies were done with plasmid DNA (~2.6 kilobase (kb)) in JM109 E. coli cells. Second, known methods do not typically transport large plasmids (>15 kb). We demonstrate here that transformation of large pVIB plasmids (i.e., >20 kb) were enhanced over water controls by ~10-fold. These results are in striking contrast to the normal decrease in transformation with increasing plasmid size.
authors.affiliation: Department of Chemistry & Biochemistry, and Center for Nanoscience, University of Missouri-St. Louis, St. Louis, Missouri 63121, USA.
authors.forename: Jason L
authors.initials: JL
authors.lastname: Atkins
authors.forename: Mohit B
authors.initials: MB
authors.lastname: Patel
authors.forename: Megan M
authors.initials: MM
authors.lastname: Daschbach
authors.forename: Joseph W
authors.initials: JW
authors.lastname: Meisel
authors.forename: George W
authors.initials: GW
authors.lastname: Gokel
mesh_terms: D000577:Amides / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics*; D010848:Picolinic Acids / Q000737:chemistry*; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D000577:Amides; D004337:Drug Carriers; D010848:Picolinic Acids; D004247:DNA; C003142:picolinamide