title: Purification of chicken breast protein hydrolysate and analysis of its antioxidant activity.
abstract: Chicken breast protein was hydrolyzed by papain under optimal conditions. The antioxidant activity of the chicken breast protein hydrolysate was then evaluated in vitro and in vivo using different measurements, including reducing power and DPPH radical scavenging assays. The reducing power of the hydrolysate was 0.5 at 2.37 mg/mL. The DPPH radical scavenging assay showed that the EC50 value of the hydrolysate was 1.28 mg/mL. In antioxidant assays in vivo, d-galactose-induced aging mice administrated the fraction peptides of chicken breast protein hydrolysate showed significantly increased antioxidant enzyme activities, while malondialdehyde levels decreased both in serums and livers. Under a transmission electron microscope (TEM), the ultramicrostructure of hepatic tissue was observed and we found that the hydrolysate may play a part in inhibiting oxidative stress in hepatocytes in vivo. Therefore, we concluded that chicken breast protein hydrolysate exhibits significant antioxidant activity.
authors.affiliation: Food Science and Nutrition Department, Nanjing Normal University, Nanjing 210097, PR China.
authors.forename: Yangying
authors.initials: Y
authors.lastname: Sun
authors.forename: Daodong
authors.initials: D
authors.lastname: Pan
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Guo
authors.forename: Junjiang
authors.initials: J
authors.lastname: Li
mesh_terms: D051046:Amino-Acid N-Acetyltransferase / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002645:Chickens; D006868:Hydrolysis; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009124:Muscle Proteins / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry*; D010206:Papain; D010851:Picrates
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D009124:Muscle Proteins; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl; D051046:Amino-Acid N-Acetyltransferase; D010206:Papain