title: Crystallization studies of the murine c-di-GMP sensor protein STING.
abstract: The innate immune response is the first defence system against pathogenic microorganisms, and cytosolic detection of pathogen-derived DNA is believed to be one of the major mechanisms of interferon production. Recently, the mammalian ER membrane protein STING (stimulator of IFN genes; also known as MITA, ERIS, MPYS and TMEM173) has been found to be the master regulator linking the detection of cytosolic DNA to TANK-binding kinase 1 (TBK1) and its downstream transcription factor IFN regulatory factor 3 (IRF3). In addition, STING itself was soon discovered to be a direct sensor of bacterial cyclic dinucleotides such as c-di-GMP or c-di-AMP. However, structural studies of apo STING and its complexes with these cyclic dinucleotides and with other cognate binding proteins are essential in order to fully understand the roles played by STING in these crucial signalling pathways. In this manuscript, the successful crystallization of the C-terminal domain of murine STING (STING-CTD; residues 138-344) is reported. Native and SeMet-labelled crystals were obtained and diffracted to moderate resolutions of 2.39 and 2.2 Å, respectively.
authors.affiliation: Institute of Biochemistry, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Yi-Che
authors.initials: YC
authors.lastname: Su
authors.forename: Zhi-Le
authors.initials: ZL
authors.lastname: Tu
authors.forename: Chao-Yu
authors.initials: CY
authors.lastname: Yang
authors.forename: Ko-Hsin
authors.initials: KH
authors.lastname: Chin
authors.forename: Mary Lay-Cheng
authors.initials: ML
authors.lastname: Chuah
authors.forename: Zhao-Xun
authors.initials: ZX
authors.lastname: Liang
authors.forename: Shan-Ho
authors.initials: SH
authors.lastname: Chou
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D000099197:STING Protein
chemical_list: D008565:Membrane Proteins; C530855:Sting1 protein, mouse; D000099197:STING Protein