title: Crystallization and preliminary X-ray analysis of the receiver domain of a putative response regulator, BPSL0128, from Burkholderia pseudomallei.
abstract: bpsl0128, a gene encoding a putative response regulator from Burkholderia pseudomallei strain D286, has been cloned into a pETBLUE-1 vector system, overexpressed in Escherichia coli and purified. The full-length protein is degraded during purification to leave a fragment corresponding to the putative receiver domain, and crystals of this protein that diffracted to beyond 1.75 Å resolution have been grown by the hanging-drop vapour-diffusion technique using PEG 6000 as the precipitant. The crystals belonged to one of the enantiomorphic pair of space groups P3(1)21 and P3(2)21, with unit-cell parameters a = b = 65.69, c = 105.01 Å and either one or two molecules in the asymmetric unit.
authors.affiliation: Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, England.
authors.forename: Abd Ghani
authors.initials: AG
authors.lastname: Abd Aziz
authors.forename: Svetlana E
authors.initials: SE
authors.lastname: Sedelnikova
authors.forename: Sergey N
authors.initials: SN
authors.lastname: Ruzheinikov
authors.forename: Simon
authors.initials: S
authors.lastname: Thorpe
authors.forename: Rahmah
authors.initials: R
authors.lastname: Mohamed
authors.forename: Sheila
authors.initials: S
authors.lastname: Nathan
authors.forename: John B
authors.initials: JB
authors.lastname: Rafferty
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Baker
authors.forename: David W
authors.initials: DW
authors.lastname: Rice
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D016957:Burkholderia pseudomallei / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D026901:Membrane Transport Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins