title: Producing high sugar concentrations from loblolly pine using wet explosion pretreatment.
abstract: We present quantitative analysis of pretreatment for obtaining high conversion and release of sugars from loblolly pine. We use wet explosion (WEx): wet oxidation followed by steam explosion and enzymatic hydrolysis (EH) at high dry matter to solubilize sugars. WEx was conducted at 25% (w/w) solids in presence of oxygen at pressures 6.5-7.2 bar, temperatures 170-175°C and residence time from 20 to 22.5 min. EH of pretreated samples was performed by Cellic® Ctec2 (60 mg protein/g cellulose) and Cellic® Htec2 enzymes (10% of Ctec2) at 50°C for 72 h. At the optimal WEx condition 96% cellulose and nearly 100% hemicellulose yield were obtained. The final concentrations of monomeric sugars were 152 g/L of glucose, 67 g/L of xylose, and 67 g/L of minor sugars (galactose, arabinose and mannose). Compared to previous work WEx seems to be superior for releasing high concentrations of monomeric sugars.
authors.affiliation: Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University, Richland, WA 99354-1671, USA.
authors.forename: Diwakar
authors.initials: D
authors.lastname: Rana
authors.forename: Vandana
authors.initials: V
authors.lastname: Rana
authors.forename: Birgitte K
authors.initials: BK
authors.lastname: Ahring
mesh_terms: D056804:Biofuels; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D009005:Monosaccharides / Q000302:isolation & purification* / Q000378:metabolism; D010084:Oxidation-Reduction; D041603:Pinus taeda / Q000737:chemistry*; D013227:Steam; D013696:Temperature
chemical_list: D056804:Biofuels; D009005:Monosaccharides; D013227:Steam; C036909:lignocellulose; D008031:Lignin