title: Direct observation of mammalian cell growth and size regulation.
abstract: We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.
authors.affiliation: Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
authors.forename: Sungmin
authors.initials: S
authors.lastname: Son
authors.forename: Amit
authors.initials: A
authors.lastname: Tzur
authors.forename: Yaochung
authors.initials: Y
authors.lastname: Weng
authors.forename: Paul
authors.initials: P
authors.lastname: Jorgensen
authors.forename: Jisoo
authors.initials: J
authors.lastname: Kim
authors.forename: Marc W
authors.initials: MW
authors.lastname: Kirschner
authors.forename: Scott R
authors.initials: SR
authors.lastname: Manalis
mesh_terms: D000818:Animals; D049108:Cell Enlargement; D002460:Cell Line; D019070:Cell Lineage; D049109:Cell Proliferation; D048429:Cell Size; D016193:G1 Phase; D008214:Lymphocytes / Q000166:cytology*; D051379:Mice; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D054448:Precursor Cells, B-Lymphoid / Q000166:cytology*; D016196:S Phase; D059010:Single-Cell Analysis / Q000379:methods*