title: Alteration of plasma galactose/N-acetylgalactosamine level after irradiation.
abstract: Although glycoproteins possess a variety of functional and structural roles in intracellular and intercellular activities, the effect of ionizing radiation (IR) on glycosylation is largely unknown. To explore this effect, we established a sandwich assay in which PHA-L, a phytohaemagglutinin that agglutinates leukocytes, was used as a coating layer to capture glycoproteins containing complex oligosaccharides; the bound glycoproteins were then measured. C57BL/6 mice were exposed to 0, 3, 6, or 10 Gy, and the plasma was collected at 6, 12, 18, 24, 48, 72, or 168 h and then analyzed for galactose/N-acetylgalactosamine (Gal/GalNAc) containing proteins. We found that (1) the sandwich assay accurately measured the level of glycoproteins, (2) 6-12 h after IR, the amount of glycoproteins containing GalNAc increased, and (3) at 72 and 168 h, 10 Gy was associated with a decrease in Gal/GalNAc. These IR-induced alterations might relate to the release of glycoproteins into the blood and the damage of the proteins and genes that are related to the glycosylation process.
authors.affiliation: Institute of Digestive Diseases, Zhengzhou University, Henan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Deping
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Chun
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Bingrong
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Zhenhuan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Shanmin
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Yansong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Okunieff
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA. lurongzhang@ufl.edu.
authors.forename: Lurong
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000116:Acetylgalactosamine / Q000031:analogs & derivatives / Q000097:blood*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005690:Galactose / Q000097:blood*; D006023:Glycoproteins / Q000097:blood*; D006031:Glycosylation / Q000528:radiation effects*; D008297:Male; D008358:Mannose / Q000097:blood*; D051379:Mice; D008810:Mice, Inbred C57BL; D010835:Phytohemagglutinins / Q000378:metabolism; D014916:Whole-Body Irradiation
chemical_list: D006023:Glycoproteins; D010835:Phytohemagglutinins; C100156:leukoagglutinins, plants; D000116:Acetylgalactosamine; D008358:Mannose; D005690:Galactose