title: [Effects of resveratrol on apoptosis and ROS production in Hepa 1-6 hepatocarcinoma cells].
abstract: OBJECTIVE
  To observe the effects of resveratrol on apoptosis and ROS production in murine hepatocarcinoma Hepa 1-6 cells in vitro.
  
  
  METHODS
  Hepa 1-6 cells were treated with different dose of resveratrol (20 micromol/L, 40 micromol/L, 80 micromol/L). Cell proliferation was detected with MTT assay at 24 h, 48 h and 72 h. Hoechst 33258 staining was used to visualize apoptotic morphology. Cell apoptosis was detected by flow cytometry analysis. Activated caspase-3 was detected by western blot. Intracellular ROS production was observed by 2',7'-dichlorofluorescin diacetate (DCF-DA) staining.
  
  
  RESULTS
  Compared with the control, upon treatment with 20-80 micromol/L resveratrol for 24 h, 48 h or 72 h, the proliferation of Hepa 1-6 cells was significantly inhibited in a time-dose dependent manner. 20-80 micromol/L resveratrol also induced apoptosis and apoptotic morphology change in Hepa 1-6 cells accompanied with caspase-3 activation and ROS generation.
  
  
  CONCLUSIONS
  Resveratrol could inhibit cell proliferation, induce apoptosis in murine hepatocarcinoma Hepa 1-6 cells, and the mechanism may associated with caspase-3 activation and ROS production.
authors.affiliation: Department of Oncology, Institute of Traditional Chinese Medicine in Oncology, Long-hua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. lsduqin123@126.com
authors.forename: Qin
authors.initials: Q
authors.lastname: Du
authors.forename: Ke-Ping
authors.initials: KP
authors.lastname: Shen
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
authors.forename: Shan
authors.initials: S
authors.lastname: Deng
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D017382:Reactive Oxygen Species; D013267:Stilbenes; D053148:Caspase 3; D000077185:Resveratrol