title: Isolation of human eosinophils.
abstract: Highly purified eosinophils can be isolated from peripheral blood by negative selection using an antibody-based magnetic negative selection protocol. The basic protocol describes a sequential fractionation of peripheral blood in which CD16+ granulocytes are enriched first from whole blood, followed by isolation of eosinophils. This technique is easy to use, fast, and highly reproducible. Support protocols describe a staining methods that can be used to evaluate the purity of eosinophils and differentiation from other leukocyte populations.
authors.affiliation: Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts.
authors.forename: Praveen
authors.initials: P
authors.lastname: Akuthota
authors.affiliation: Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts.
authors.forename: Revital
authors.initials: R
authors.lastname: Shamri
authors.affiliation: Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts.
authors.forename: Peter F
authors.initials: PF
authors.lastname: Weller
mesh_terms: D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D004804:Eosinophils / Q000166:cytology* / Q000276:immunology; D006098:Granulocytes / Q000166:cytology* / Q000276:immunology; D006801:Humans; D008280:Magnetics; D017452:Receptors, IgG / Q000276:immunology; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods
chemical_list: D017452:Receptors, IgG