title: Simultaneous determination and validated quantification of human insulin and its synthetic analogues in human blood serum by immunoaffinity purification and liquid chromatography-mass spectrometry.
abstract: Possible fatal complications of human insulin and its synthetic analogues like hypoglycemia require precise classification and quantitative determination of these drugs both for clinical purposes as well as for forensic toxicologists. A procedure was developed for the identification and quantification of human insulin and different long-acting as well as short-acting synthetic insulins in human blood serum specimens. After an immunoaffinity purification step and separation by liquid chromatography, the insulins were characterized by their five- or sixfold protonated molecule ions and diagnostic product ions. Clinical samples of 207 diabetic and 50 non-diabetic patients after the administration of human insulin or oral antidiabetics and forensic samples were analyzed for human/synthetic insulin concentrations. The method was validated according to international guidelines. Limits of detection of the insulins ranged between 1.3 and 2.8 μU/ml. Recoveries ranged between 33.2 % and 51.7 %. Precision data was in accordance with international guidelines. Clinical samples showed concentrations of human insulin lower than 301 μU/ml. Our liquid chromatography tandem mass spectrometry procedure allows unambiguous identification and quantification of the intact human insulin and its intact synthetic analogues Humalog®, Novolog®, Apidra®, Lantus®, and Levemir® in human blood serum in clinical and overdose cases. The assay could be successfully tested in patients with diabetes mellitus on therapy with insulins or oral antidiabetics.
authors.affiliation: Institute of Forensic Medicine, University Hospital Bonn, Bonn, Germany. cohess@uni-bonn.de
authors.forename: Cornelius
authors.initials: C
authors.lastname: Hess
authors.forename: Andreas
authors.initials: A
authors.lastname: Thomas
authors.forename: Mario
authors.initials: M
authors.lastname: Thevis
authors.forename: Bernd
authors.initials: B
authors.lastname: Stratmann
authors.forename: Wulf
authors.initials: W
authors.lastname: Quester
authors.forename: Diethelm
authors.initials: D
authors.lastname: Tschoepe
authors.forename: Burkhard
authors.initials: B
authors.lastname: Madea
authors.forename: Frank
authors.initials: F
authors.lastname: Musshoff
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007328:Insulin / Q000031:analogs & derivatives / Q000097:blood* / Q000138:chemical synthesis / Q000302:isolation & purification; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged; D055815:Young Adult
chemical_list: D007328:Insulin