title: Changes of CD8⁺ T cells in dextran sulfate sodium-induced colitis mice pretreated with oral immune regulation.
abstract: BACKGROUND
  It has been reported that CD8(+) regulatory cells could be induced upon oral tolerance. The purpose of this study was to investigate the changes of CD8α(+) T cells in dextran sulfate sodium (DSS)-induced colitis mice pretreated by oral immune regulation.
  
  
  METHODS
  The effects of five low oral doses of colitis-extracted proteins (CEP) on colitis were evaluated by clinical manifestation and histological lesions. The percentages of CD8α(+) T cells gating on CD3(+) T cells were evaluated in the gut-associated lymphoid tissues (GALT) and the spleens by flow cytometry. Differences between the two groups were compared by Student's t test or Mann-Whitney U test.
  
  
  RESULTS
  Compared to bovine serum albumin (BSA)-fed control mice, administration of CEP resulted in marked alleviation of colitis. The proportion of CD8α(+) T cells, not only in intraepithelial lymphocytes (IELs) and lamina propria lymphocytes (LPLs) of the large intestine (LI) but also in spleen from CEP-fed colitis mice, was significantly higher than that from BSA-fed colitis mice (LI-IELs: (71.5 ± 5.4)% vs. (60.1 ± 4.3)%, P < 0.01; LI-LPLs: (60.7 ± 5.2)% vs. (51.9 ± 4.7)%, P < 0.01; spleen: (24.1 ± 3.6)% vs. (20.3 ± 4.1)%, P < 0.05; n = 8). Mucosal repair in repair-period mice five days after termination of DSS treatment was also accompanied by an increase of CD8α(+) T cells in large intestinal mucosal lymphocytes (LI-IELs: (72.1 ± 3.7)% vs. (61.5 ± 4.5)%, P < 0.01; LI-LPLs: (62.1 ± 5.7)% vs. (52.7 ± 3.6)%, P < 0.01; n = 8). The proportion of CD3(+) T cells increased in Peyer's patches (PPs) and decreased in mesenteric lymph nodes (MLNs) from colitis mice compared to untreated mice, whereas the change pattern of CD3(+) T cells in PPs and MLNs from CEP-fed colitis mice was just on the contrary.
  
  
  CONCLUSIONS
  Improvement of DSS-induced colitis resulted from oral immune regulation is associated with an increase in CD8α(+) T cells in spleen and large intestinal mucosa.
authors.affiliation: Department of Gastroenterology, Affiliated Hospital, Hangzhou Normal University College of Medicine, Hangzhou, Zhejiang 310015, China.
authors.forename: Yue-Fang
authors.initials: YF
authors.lastname: Ye
authors.forename: Xi
authors.initials: X
authors.lastname: Jin
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Chen
authors.forename: Min
authors.initials: M
authors.lastname: Yue
authors.forename: You-Ming
authors.initials: YM
authors.lastname: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced* / Q000150:complications*; D016264:Dextran Sulfate / Q000633:toxicity*; D005434:Flow Cytometry; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011506:Proteins / Q000008:administration & dosage* / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000378:metabolism
chemical_list: D011506:Proteins; D016264:Dextran Sulfate