title: Characterization of the lipid accumulation in a tropical freshwater microalgae Chlorococcum sp.
abstract: A green microalgae strain, Chlorococcum sp. was obtained from tropical freshwater in Indonesia. The effects of pH on growth, effects of salt, carbon dioxide level, nitrate, acetate, and photoperiod on biomass and lipid production were studied. The fatty acids composition was also investigated. This study shows that the strain had an optimum pH value for growth ranging from pH 8.0 to 8.5. The biomass concentration and lipid content were influenced by different concentrations of salt, CO(2) and nitrate. The lipid productivity ranged from 2 to 90.8 mg L(-1)d(-1) in different mediums. The highest biomass concentration and total lipid content achieved were 1.75 g L(-1) and 56% of dry weight, respectively. Moreover, the major fatty acid methyl esters were C16:0, C18:1, C18:2 and C18:3. The high lipid content and the fatty acid composition make the strain Chlorococcum sp. a potential resource for food, cosmetics and biodiesel.
authors.affiliation: Institute of Agricultural Technology and Biosystems Engineering, Johann Heinrich von Thünen Institut (vTI), Bundesallee 50, D-38116 Braunschweig, Germany. umi.harwati@vti.bund.de
authors.forename: Theresia Umi
authors.initials: TU
authors.lastname: Harwati
authors.forename: Thomas
authors.initials: T
authors.lastname: Willke
authors.forename: Klaus D
authors.initials: KD
authors.lastname: Vorlop
mesh_terms: D000085:Acetates / Q000032:analysis; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002245:Carbon Dioxide / Q000032:analysis; D000460:Chlorophyta / Q000254:growth & development / Q000378:metabolism*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005618:Fresh Water / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007214:Indonesia; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D009566:Nitrates / Q000032:analysis; D017440:Photoperiod; D013053:Spectrophotometry; D014329:Tropical Climate
chemical_list: D000085:Acetates; D008055:Lipids; D009566:Nitrates; D002245:Carbon Dioxide