title: OxLDL- and HSP-60 antigen-specific CD8(+) T lymphocytes are detectable in the peripheral blood of patients suffering from coronary artery disease.
abstract: Inflammatory and immunologic mechanisms are important for the initiation and the progression of atherosclerotic lesions. OxLDL and HSP-60 antigens are involved in the pathogenesis of atherosclerotic disease by triggering immune cells within the plaques. Through the MHC pentamer assays, we investigated the presence of OxLDL- and HSP-60-specific CD8(+) T lymphocytes in twenty HLA-A2-positive patients suffering from coronary artery disease (10 NSTEMI and 10 stable angina). Similarly, 10 age- and sex-matched healthy subjects were enrolled as controls. Biological samples were collected within 6 h of admission to hospital, at 30 days and at 180 days. OxLDL- and HSP-60-specific CD8(+) T lymphocytes were never detectable in the peripheral blood from all the healthy controls. On the contrary, at each scheduled time point, both of these specific cells could be detected in peripheral blood from all enrolled patients. More in detail, the flow cytometric analysis of MHC-1 pentamer OxLDL-specific CD8(+) T lymphocytes revealed a sharp and significant increase at the hospital admission, within 6 h from the chest pain onset, followed by an evident decline to lower levels at 30 days and at 180 days from the enrollment in the study. On the contrary, although MHC-1 pentamer HSP-60 CD8(+) T lymphocytes were detectable in enrolled patients, almost no variance could be detectable during the follow-up scheduled evaluations. On the whole, this finding indicates that HSP-60- and OxLDL-specific CD8(+) T lymphocytes could play a role in the maintenance or worsening of the atherosclerotic coronary disease.
authors.affiliation: Department of Internal Medicine (DiMI) and Medical Specialties, I.R.C.C.S. "A.O.U. San Martino-IST", University of Genoa, Viale Benedetto XV, 6, 16132, Genoa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Ghio
authors.forename: P
authors.initials: P
authors.lastname: Fabbi
authors.forename: P
authors.initials: P
authors.lastname: Contini
authors.forename: M
authors.initials: M
authors.lastname: Fedele
authors.forename: C
authors.initials: C
authors.lastname: Brunelli
authors.forename: F
authors.initials: F
authors.lastname: Indiveri
authors.forename: A
authors.initials: A
authors.lastname: Barsotti
mesh_terms: D000328:Adult; D000368:Aged; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018834:Chaperonin 60 / Q000276:immunology*; D003324:Coronary Artery Disease / Q000276:immunology* / Q000473:pathology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008077:Lipoproteins, LDL / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000276:immunology*
chemical_list: D018834:Chaperonin 60; C060293:HSPD1 protein, human; D008077:Lipoproteins, LDL; D024101:Mitochondrial Proteins; C099252:oxidized low density lipoprotein