title: SCC-S2 is overexpressed in colon cancers and regulates cell proliferation.
abstract: SCC-S2 was recently reported to be overexpressed in various cancers and associated with the malignant behavior of cancer cells. However, the expression of SCC-S2 and its biological roles in colon cancers have not been reported. The aim of this study is to investigate the expression and clinical significance of SCC-S2 in colon cancers and explore its biological function in colon cancer cells. We analyzed the expression pattern of SCC-S2 in 92 colon cancer tissues by immunohistochemistry and found that SCC-S2 was overexpressed in 45 (48.9 %) of 92 colon cancer specimens. There was a significant association between SCC-S2 overexpression and TNM stage (p = 0.0387), lymph node metastasis (p = 0.0219), and proliferation index (p = 0.0279). siRNA knockdown of SCC-S2 expression in CACO2 and HCT116 cells decrease cell proliferation, colony formation, and soft agar colony formation ability. Furthermore, western blot analysis showed that SCC-S2 depletion decreased cyclin D1 and phospho-Rb levels. In conclusion, we demonstrated that SCC-S2 is overexpressed in colon cancers and contributes to malignant cell growth by cyclin D1 and phospho-Rb modulation, which makes SCC-S2 a candidate therapeutic target in colon cancer.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of China Medical University, Shenyang, China.
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Miao
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.forename: Zhenning
authors.initials: Z
authors.lastname: Wang
authors.forename: Yingying
authors.initials: Y
authors.lastname: Xu
authors.forename: Yongxi
authors.initials: Y
authors.lastname: Song
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wu
authors.forename: Huimian
authors.initials: H
authors.lastname: Xu
mesh_terms: D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D018938:Caco-2 Cells; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005260:Female; D005434:Flow Cytometry; D045325:HCT116 Cells; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051017:Apoptosis Regulatory Proteins; D011506:Proteins; D012333:RNA, Messenger; C404190:TNFAIP8 protein, human