title: Suillus collinitus methanolic extract increases p53 expression and causes cell cycle arrest and apoptosis in a breast cancer cell line.
abstract: In the present work, methanolic, ethanolic and boiled water extracts of Suillus collinitus were chemically characterised and submitted to an evaluation of their bioactive properties (antioxidant potential and cytotoxic activity in tumor cell lines). Phenolic acids and sugars were identified chromatographically and quantified in the methanolic and boiled water extracts, respectively. S. collinitus ethanolic extract had the highest antioxidant activity. Nevertheless, with respect to cell growth inhibition, the methanolic extract was the most potent extract, particularly in MCF-7 cells (GI(50) 25.2±0.2 μg/ml). Moreover, the GI(50) concentration of this extract induced a G1 cell cycle arrest, with a concomitant decrease in the percentage of cells in the S phase. Furthermore, it caused an increase in the percentage of apoptotic cells, from 6.0±0.2% in untreated cells, to 15.3±2.0% in cells treated with the GI(50) concentration and to 16.3±2.0% in cells treated with 2×GI(50) concentration. In addition, 48 h treatment with the GI(50) concentration caused a strong increase in the levels of p53, p21, and cleaved PARP, together with a decrease in Bcl-2 and XIAP. Results indicate that S. collinitus may be a promising source of bioactive compounds. Particularly, its methanolic extract appears to have a p53-mediated effect on the normal cell cycle distribution and apoptosis induction in a human breast tumor cell line.
authors.affiliation: Cancer Drug Resistance Group, IPATIMUP - Institute of Molecular Pathology and Immunology of the University of Porto, Portugal.
authors.forename: Josiana A
authors.initials: JA
authors.lastname: Vaz
authors.forename: Isabel C F R
authors.initials: IC
authors.lastname: Ferreira
authors.forename: Catarina
authors.initials: C
authors.lastname: Tavares
authors.forename: Gabriela M
authors.initials: GM
authors.lastname: Almeida
authors.forename: Anabela
authors.initials: A
authors.lastname: Martins
authors.forename: M
authors.initials: M
authors.lastname: Helena Vasconcelos
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001487:Basidiomycota / Q000737:chemistry*; D001685:Biological Factors / Q000302:isolation & purification / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D062385:Hydroxybenzoates / Q000302:isolation & purification / Q000494:pharmacology; D061986:MCF-7 Cells; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D001685:Biological Factors; D062385:Hydroxybenzoates; D016159:Tumor Suppressor Protein p53; C017616:phenolic acid