title: A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures.
abstract: Outbreaks caused by vaccine-derived polioviruses are challenging the final eradication of paralytic poliomyelitis. Therefore, the surveillance of the acute flaccid paralysis cases based on poliovirus isolation and characterization remains an essential activity. Due to the use of trivalent oral poliovirus vaccine (OPV), mixtures containing more than one serotype of Sabin-related polioviruses are frequently isolated from clinical samples. Because each poliovirus isolate needs to be individually analyzed, we designed polymerase chain reaction primers that can selectively distinguish and amplify a genomic segment of the three Sabin-related poliovirus serotypes present in mixtures, thus, optimizing the diagnosis and providing prompt information to support epidemiologic actions.
authors.affiliation: Laboratório de Enterovírus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brasil.
authors.forename: Eliane Veiga da
authors.initials: EV
authors.lastname: Costa
authors.forename: Renata de Mendonça
authors.initials: Rde M
authors.lastname: Campos
authors.forename: Fernando Neto
authors.initials: FN
authors.lastname: Tavares
authors.forename: Cátia Regina Valério
authors.initials: CR
authors.lastname: Grégio
authors.forename: Fernanda Marcicano
authors.initials: FM
authors.lastname: Burlandy
authors.forename: Edson Elias da
authors.initials: EE
authors.lastname: Silva
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D016679:Genome, Viral; D006801:Humans; D009154:Mutation; D010641:Phenotype; D011051:Poliomyelitis / Q000276:immunology / Q000821:virology*; D017955:Poliovirus / Q000235:genetics* / Q000276:immunology; D011055:Poliovirus Vaccine, Oral / Q000235:genetics* / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D011055:Poliovirus Vaccine, Oral