title: Trypan blue staining of filtering bleb in eyes with operate trabeculectomy.
abstract: OBJECTIVE
  To report the use of trypan blue staining of the filtering bleb to assess its functional status in eyes undergoing phacoemulsification after trabeculectomy.
  
  
  METHODS
  This retrospective study was conducted at a tertiary eye care centre in North India and studied 33 eyes of 33 patients ( with previously operated trabeculectomy), who underwent phacoemulsification. Trypan blue dye (0.06%) was used to stain the anterior capsule. After completion of phacoemulsification, the staining of the trabeculectomy bleb was noted as diffuse, patchy, minimal or no staining.
  
  
  RESULTS
  Of the 33 eyes, 13 had diffuse staining (39.4%, mean IOP = 9.3 ± 2.2 mm Hg), 7 (21.2%, mean IOP= 15.5 ± 1.8 mm Hg) had patchy staining, 4 had minimal staining (12.1%, mean IOP= 17.5 ± 0.5mm Hg) and nine (27.3%, mean IOP= 19.3 ± 1.6 mm Hg) had no staining. These staining patterns were labeled as groups 1 - 4 respectively. Statistical analysis showed that the difference between the IOPs in Group 1 - 2 and between Group 2 - 3 was not significant statistically (p=0.682 and 0.665 respectively). However the differences between the IOPs between Groups 1 - 3, 1 - 4, 2 - 4, and 3 - 4 were found to be highly significant statistically (p less than 0.0005).
  
  
  CONCLUSIONS
  Trypan blue dye can be used to test the amount of sub conjunctival filtration in eyes undergoing phacoemulsification cataract surgery.
authors.affiliation: Dr Rajendra Prasad Centre for Ophthalmic sciences, AIIMS, New Delhi, India. tanujdada@gmail.com
authors.forename: T
authors.initials: T
authors.lastname: Dada
authors.forename: S J
authors.initials: SJ
authors.lastname: Bali
authors.forename: S
authors.initials: S
authors.lastname: Mohan
authors.forename: S
authors.initials: S
authors.lastname: Bhartiya
authors.forename: A
authors.initials: A
authors.lastname: Sobti
authors.forename: A
authors.initials: A
authors.lastname: Panda
mesh_terms: D000867:Anterior Chamber; D002386:Cataract / Q000150:complications; D004396:Coloring Agents / Q000008:administration & dosage; D003228:Conjunctiva / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D005901:Glaucoma / Q000150:complications / Q000175:diagnosis* / Q000601:surgery; D006801:Humans; D007267:Injections; D007429:Intraocular Pressure / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D018918:Phacoemulsification / Q000379:methods; D012189:Retrospective Studies; D012590:Sclera / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods; D014130:Trabeculectomy / Q000379:methods*; D016896:Treatment Outcome; D014343:Trypan Blue / Q000008:administration & dosage
chemical_list: D004396:Coloring Agents; D014343:Trypan Blue