title: An improved AhR reporter gene assay for analyzing dioxins in soil, sediment and fish.
abstract: Our goal was to develop a fast-screening bioassay to determine dioxin levels in the environmental and biological samples from dioxin-contaminated areas. Our original dioxin-responsive-element (DRE)-driven luciferase bioassay (using Huh7-DRE-Luc cells) was modified by reducing the incubation temperature of the cell culture from 37 to 35°C and by adding phorbol-12-myristate-13-acetate, and the modified bioassay was used to examine samples from soil, sediment, and fish. The results of this bioassay were shown to be significantly related to those of the high-resolution gas chromatography/high-resolution mass spectrometry assay of dioxins. The correlative equation was: log (PCDD/Fs I-TEQs) = 1.19 × log (BEQs) - 1.15 with R(2) = 0.95 (p < 0.001).
authors.affiliation: Department of Environmental Science and Engineering, Emerging Compounds Research Center, National Pingtung University and Science and Technology, Neipu 912, Pingtung County, Taiwan.
authors.forename: How-Ran
authors.initials: HR
authors.lastname: Chao
authors.forename: Ya-Fan
authors.initials: YF
authors.lastname: Wang
authors.forename: Yao-Nan
authors.initials: YN
authors.lastname: Wang
authors.forename: Ding-Yan
authors.initials: DY
authors.lastname: Lin
authors.forename: Yan-You
authors.initials: YY
authors.lastname: Gou
authors.forename: Chien-Yu
authors.initials: CY
authors.lastname: Chen
authors.forename: Kuan-Chung
authors.initials: KC
authors.lastname: Chen
authors.forename: Wen-Kai
authors.initials: WK
authors.lastname: Wu
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Chiang
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Huang
authors.forename: Lien-Te
authors.initials: LT
authors.lastname: Hsieh
authors.forename: Kuei-Jyum C
authors.initials: KJ
authors.lastname: Yeh
authors.forename: Tsui-Chun
authors.initials: TC
authors.lastname: Tsou
mesh_terms: D000818:Animals; D001681:Biological Assay; D015415:Biomarkers / Q000378:metabolism; D004147:Dioxins / Q000032:analysis / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods*; D005399:Fishes; D017930:Genes, Reporter; D019015:Geologic Sediments / Q000737:chemistry; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics* / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D004147:Dioxins; D018336:Receptors, Aryl Hydrocarbon; D012989:Soil Pollutants