title: [Effects of total flavonoids in Drynaria fortunei on osteoblasts differentiation and the expression of ERK1/2 and p38 MAPK after treatment by high glucose in vitro].
abstract: OBJECTIVE
  To observe the effects of Total Flavonoids in Drynaria fortunei (TFDF) on osteoblasts differentiation activity after treatment by high glucose and observe the effects on p38MAPK and ERK1/2 signaling protein in osteoblasts.
  
  
  METHODS
  Primary osteoblasts of newborn SD rats was extracted and cultured and its biological characteristics was observed. MTT method was used to observe osteoblasts' cytotoxicity,and to choose a suitable concentration of TFDF in the culture medium. pNPP,ELISA,Alizarin dyeing were used to test ALP,Type I collagen,osteocalcin and mineralization of osteoblasts after treatment by different concentration of glucose respectively and after treatment by TFDF and high glucose. Western-blot was used to detect p38MAPK and ERK1/2 protein phosphorylation after treatment by TFDF and high glucose.
  
  
  RESULTS
  Primary osteoblasts of newborn SD rats could be used well in this experiment. According to the toxicity of TFDF on OB, 25, 50, 100 mg/L of TFDF were selected for the experimental concentration gradient. ALP, Type I collagen,osteocalcin and mineralization of osteoblasts after treatment with glucose (25, 50 mmol/L) were less than those of control group respectively. TFDF could increase ALP, Type I collagen, osteocalcin activity and mineralization of osteoblasts in a dose-dependent manner after treatment by high glucose (25 mmol/L). TFDF(50 mg/L) could increase protein phosphorylation of p38MAPK and ERK1/2 of osteoblasts after treatment by high glucose (25 mmol/L).
  
  
  CONCLUSIONS
  High glucose can decrease differentiation and mineralization of osteoblasts. TFDF can increase differentiation and mineralization of osteoblasts in a dose-dependent manner after treatment by high glucose. The role of TFDF in the promotion of osteoblasts differentiation is related to protein phosphorylation of p38MAPK and ERK1/2.
authors.affiliation: Traditional Chinese Medicine Department, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China. zxiaof@jnu.edu.cn
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Zhu
authors.forename: Ting-Chun
authors.initials: TC
authors.lastname: Wang
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Zhang
authors.forename: Sheng-Yun
authors.initials: SY
authors.lastname: Sun
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.forename: Ling
authors.initials: L
authors.lastname: Jin
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D015675:Osteocalcin / Q000378:metabolism; D010024:Osteoporosis / Q000517:prevention & control; D029621:Polypodiaceae / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D012886:Skull / Q000166:cytology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D005419:Flavonoids; D015675:Osteocalcin; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase; D005947:Glucose