title: [Mutational analysis of candidate genes in a Chinese pedigree with dominantly inherited auditory neuropathy].
abstract: OBJECTIVE
  Three genes including the OTOF, the DFNB59 and the DIAPH3 have been implicated previously in human non-syndromic auditory neuropathy. In this study, we aim to investigate whether DIAPH3 gene or the known deafness loci of 25 cloned autosomal dominant deafness (DFNA) genes contribute to the nonsyndromic hearing loss of a Chinese pedigree with dominantly inherited auditory neuropathy (AN).
  
  
  METHODS
  Nine members of the kernal pedigree in this family were selected. Genomic DNA was isolated from the peripheral leukocytes of the subjects using the Puregene DNA Isolation Kits. Firstly, the 5'UTR of DIAPH3 gene was PCR amplified in all subjects. Then, the DNA fragments spanning the entire coding regions of DIAPH3, GJB2 and GJB3 genes, and 50 exons in other 23 cloned DFNA genes were amplified using specific primers. Each fragment was purified and analyzed by direct sequencing. The resultant sequence data were compared with the standard sequence to identify deafness-associated mutations.
  
  
  RESULTS
  PCR amplifications were successfully conducted. We failed to detect the presence either of c. --172G > A mutation in the 5'UTR that have been reported, or any other deafness-associated mutations in the whole DIAPH3 gene, by sequence analysis. We also did not find any known deafness-causing mutations among the 25 cloned DFNA genes.
  
  
  CONCLUSIONS
  The DIAPH3 gene, and the known deafness loci of 25 cloned DFNA genes seem not contribute to the pathogenesis of this Chinese AN family in this study, which suggesting new gene(s) involvement.
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Xinhong
authors.initials: X
authors.lastname: Lu
authors.forename: Ruichun
authors.initials: R
authors.lastname: Chen
authors.forename: Yajie
authors.initials: Y
authors.lastname: Lu
authors.forename: Qinjun
authors.initials: Q
authors.lastname: Wei
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Cao
authors.forename: Guangqian
authors.initials: G
authors.lastname: Xing
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D044466:Asian People; D002681:China; D000072259:Connexin 26; D017630:Connexins; D004252:DNA Mutational Analysis; D003638:Deafness; D005091:Exons; D000080865:Formins; D034381:Hearing Loss; D006313:Hearing Loss, Central / Q000235:genetics*; D006319:Hearing Loss, Sensorineural / Q000235:genetics*; D006801:Humans; D009154:Mutation; D010375:Pedigree; D016133:Polymerase Chain Reaction
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D017630:Connexins; C484571:DIAPH3 protein, human; D000080865:Formins; C000607042:GJB2 protein, human; D000072259:Connexin 26