title: Proteome analysis of the UVB-resistant marine bacterium Photobacterium angustum S14.
abstract: The proteome of the marine bacterium Photobacterium angustum S14 was exposed to UVB and analyzed by the implementation of both the post-digest ICPL labeling method and 2D-DIGE technique using exponentially growing cells. A total of 40 and 23 proteins were quantified in all replicates using either the ICPL or 2D-DIGE methods, respectively. By combining both datasets from 8 biological replicates (4 biological replicates for each proteomics technique), 55 proteins were found to respond significantly to UVB radiation in P. angustum. A total of 8 UVB biomarkers of P. angustum were quantified in all replicates using both methods. Among them, the protein found to present the highest increase in abundance (almost a 3-fold change) was RecA, which is known to play a crucial role in the so-called recombinational repair process. We also observed a high number of antioxidants, transport proteins, metabolism-related proteins, transcription/translation regulators, chaperonins and proteases. We also discuss and compare the UVB response and global protein expression profiles obtained for two different marine bacteria with trophic lifestyles: the copiotroph P. angustum and oligotroph Sphingopyxis alaskensis.
authors.affiliation: UPMC Univ Paris 06, UMR7621, Laboratoire d'Océanographie Microbienne, Observatoire Océanologique, Banyuls/mer, France. sabine.matallana@obs-banyuls.fr
authors.forename: Sabine
authors.initials: S
authors.lastname: Matallana-Surget
authors.forename: Fabien
authors.initials: F
authors.lastname: Joux
authors.forename: Ruddy
authors.initials: R
authors.lastname: Wattiez
authors.forename: Philippe
authors.initials: P
authors.lastname: Lebaron
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects*; D010776:Photobacterium / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D013045:Species Specificity; D042301:Sphingomonadaceae / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D020543:Proteome