title: [Comparative study on tumor cell apoptosis in vitro induced by extracts of Stellera chamaejasme].
abstract: OBJECTIVE
  To in vitro compare the induction of extracts of Stellera chamaejasme ESC, ESC-1 and ESC-2 on NCI-H157 cell apoptotic.
  
  
  METHODS
  The apoptosis rate was inspected by flow cytometry; caspase-3, 8, 9 activities was measured by spectrophotometry. Fas, Fas-L, TNF-alpha, Trail-R, Cyto-C, Smac/diablo protein expressions of apoptosis pathway was observed by Elisa method.
  
  
  RESULTS
  Compared with the control group, ESC, ESC-1, ESC-2 can significantly improve the apoptosis rate of NCI-H157 cell. ESC significantly improved cells caspase-3, 8 activity, ESC-2 can significantly improve the activity of caspase-3, 8, 9. ESC, ESC-1, ESC-2 significantly increased Fas expression and ESC significantly increased Fas/Fas-L ratio. ESC, ESC-1, ESC-2 significantly increased TNF-alpha protein expression. ESC-1 significantly lowered TRAIL-R expression. ESC, ESC-1, ESC-2 had no significant effect on Cyto-C. ESC-1, ESC-2 significantly reduced Smac protein expression.
  
  
  CONCLUSIONS
  The apoptotic effect induced by ESCs may be related to the regulation of death receptor pathway proteins. Induction mechanisms of ESCs were so complicated that it may have a two-way regulatory effect. Its induction in apoptosis is a result from comprehensive regulation and control.
authors.affiliation: Beijing Institute of Liver Diseases, Beijing 100069, China. liuxiaoni888@yahoo.com.cn
authors.forename: Xiaoni
authors.initials: X
authors.lastname: Liu
authors.forename: Yujie
authors.initials: Y
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Weng
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Zhu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D009369:Neoplasms / Q000737:chemistry / Q000188:drug therapy* / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000032:analysis; D029645:Thymelaeaceae / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D019014:fas Receptor / Q000032:analysis
chemical_list: D010936:Plant Extracts; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor; D020169:Caspases