title: A generic strategy for co-presentation of heparin-binding growth factors based on CVD polymerization.
abstract: A multifunctional copolymer with both aldehyde and alkyne groups is synthesized by chemical vapor deposition (CVD) for orthogonal co-immobilization of biomolecules. Surface analytical methods including FTIR and XPS are used to confirm the surface modification. Heparin-binding growth factors [basic fibroblast growth factor (bFGF) in this study] can be immobilized through interaction with heparin, which was covalently attached to the CVD surface through an aldehyde-hydrazide reaction. In parallel, an alkyne-azide reaction is used to orthogonally co-immobilize an adhesion peptide as the second biomolecule.
authors.affiliation: Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Deng
authors.forename: Joerg
authors.initials: J
authors.lastname: Lahann
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry* / Q000378:metabolism; D005740:Gases / Q000737:chemistry; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000447:Aldehydes; D000480:Alkynes; D001386:Azides; D005740:Gases; D055767:Immobilized Proteins; D011108:Polymers; D016222:Fibroblast Growth Factor 2; D006493:Heparin