title: [Relation of genetic variants of CYP2A6 with tobacco dependence and smoking habit in Chilean subjects. A pilot study].
abstract: BACKGROUND
  Genetic and metabolic factors associated with nicotine metabolism may be related to smoking behavior.
  
  
  OBJECTIVE
  To assess the prevalence of allelic and genotype variants of CYP2A6 in a sample of Chilean subjects and to evaluate their relationship with smoking and tobacco dependence.
  
  
  METHODS
  The genotype frequencies for *2, *3 and *4 of CYP2A6*1 (wild type) gene were determined by polymerase chain reaction (PCR) in 54 volunteers. Addiction to tobacco was evaluated using the Fagerstrom Test. The association between the presence of allelic variants of CYP2A6 and smoking and tobacco dependence was evaluated with chi square test.
  
  
  RESULTS
  The prevalence of *1, *2 (wt/*2), *3 (wt/*3 or *31*3) and *4 (del/del) were 92.6%, 3.7%, 0% y 3.7%, respectively. No significant association was observed between being a carrier of a variant genotype of CYP2A6 and smoking or tobacco dependence.
  
  
  CONCLUSIONS
  In this sample of Chilean individuals we did not find a relation between any CYP2A6 genotype with smoking or tobacco dependence.
authors.affiliation: División de Epidemiología, Escuela de Salud Pública, Facultad de Medicina, Universidad de Chile, Santiago, Chile. dcaceres@med.uchile.cl
authors.forename: Dante D
authors.initials: DD
authors.lastname: Cáceres
authors.forename: Sergio A
authors.initials: SA
authors.lastname: Alvarado
authors.forename: Paulina
authors.initials: P
authors.lastname: Martínez
authors.forename: Luis A
authors.initials: LA
authors.lastname: Quiñones
mesh_terms: D000328:Adult; D000483:Alleles; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D065637:Cytochrome P-450 CYP2A6; D004247:DNA / Q000032:analysis; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D015995:Prevalence; D012907:Smoking / Q000235:genetics*; D014029:Tobacco Use Disorder / Q000235:genetics*
chemical_list: D004247:DNA; D001189:Aryl Hydrocarbon Hydroxylases; C585710:CYP2A6 protein, human; D065637:Cytochrome P-450 CYP2A6