title: Effect of Coherin™ on intraocular pressure, pupil size and heart rate in the glaucomatous Beagle: a pilot study.
abstract: OBJECTIVE
  To evaluate effects of Coherin™ on intraocular pressure (IOP), pupil size (PS), and heart rate (HR) in glaucomatous Beagles in single-dose studies in a pilot study.
  
  
  METHODS
  Intraocular pressure, PS, and HR were measured in eight glaucomatous Beagles. One randomly chosen eye received single 50 μL doses of differing concentrations of Coherin™ (treated eye) or vehicle (placebo-treated eye), and the fellow eye served as the untreated control. After the first measurements, a single dose of either Coherin™ or sterile water vehicle was instilled in the drug and placebo eyes, respectively.
  
  
  RESULTS
  The mean ± SEM diurnal changes in IOP after 0.005%, 0.01%, 0.2%, 0.284%, 1%, 2%, and 4% topical Coherin™ once daily were 7.6 ± 3.2 mmHg, 15.5 ± 5.3 mmHg, 11.2 ± 4.4 mmHg, 11.8 ± 4.4 mmHg, 19.1 ± 3.8 mmHg, 5.0 ± 1.8 mmHg, and 8.8 ± 2.8 mmHg, respectively. The declines in IOP were significantly different (P < 0.05) from the untreated control eyes with the 0.2% and 0.284% Coherin™-treated eyes and suggestive for 1% Coherin™ concentrations. No signs of irritation, significant PS, and HR changes were detected in the Coherin™-treated eyes.
  
  
  CONCLUSIONS
  Of seven different concentrations, 2% and 0.248% Coherin™ produced significant declines in IOP in the glaucomatous beagle in single-dose studies when compared to both untreated control and placebo-treated eyes. One percent Coherin™ solution produced significant IOP decreases compared with the placebo-treated eye but not the untreated control eyes. No local ocular irritation, PS and HR changes were observed in Coherin™-treated eyes. This pilot study suggests that topical Coherin™ has potential as an ocular hypotensive agent.
authors.affiliation: Department of Small Animal Clinical Sciences and Gwathmey-Adams Laboratory for Vision Science, College of Veterinary Medicine, University of Florida, PO Box 100126, Gainesville, FL 32610-0126, USA. mackaye@ufl.edu
authors.forename: Edward O
authors.initials: EO
authors.lastname: MacKay
authors.forename: Kirk N
authors.initials: KN
authors.lastname: Gelatt
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000188:drug therapy*; D004285:Dogs; D005901:Glaucoma / Q000188:drug therapy / Q000662:veterinary*; D006339:Heart Rate / Q000187:drug effects*; D007429:Intraocular Pressure / Q000187:drug effects*; D010909:Pituitary Hormones, Posterior / Q000009:adverse effects / Q000627:therapeutic use*; D011680:Pupil / Q000187:drug effects*
chemical_list: D010909:Pituitary Hormones, Posterior; C000498:coherin