title: Characterization of metalloanticancer capacity of an agglutinin from wheat.
abstract: Many anticancer drugs cannot recognize selectively tumor tissues, and cause destruction to normal ones. Although it is very toxic, cisplatin is still one of the most applied chemotherapeutics used for treatment of sarcomas, carcinomas, etc. It causes severe side effects as a result of the lack of selectivity of the drug to tumor tissue and acquired or intrinsic resistance occurs. Wheat germ agglutinin (WGA) is a lectin that specifically recognizes transformed cells: prostate cancer cells, pancreatic cells etc., and is uptaken into the tumor cells for which it appears to be a suitable target for anticancer agents. A fluorescence spectroscopy method was used to study the interaction of WGA with four metal-based anticancer drugs: cisplatin, Pt porphyrin and two gold porphyrins. The affinity constant (k(D)) for binding of cisplatin with WGA was k(D) = 6.67 ± 2.5 μM. The hyperbolic curve indicated the presence of a single cisplatin binding site. The affinity of Au and Pt porphyrin to WGA (k(D) = 0.08-0.49 μM) is almost two orders of magnitude higher than that for cisplatin. We found that Pt porphyrin could displace fluorescent dye ANS showing an increase in the fluorescence intensity with a concomitant blue shift of the emission maximum suggesting that the compounds accommodate the same binding site. Current research characterizes the metalloanticancer binding capacity of WGA. Our results indicate that four metal-based anticancer agents have high affinity for WGA. Since WGA recognizes transformed cells, the obtained data show that this protein might have putative usage as a drug delivery molecule in cancer.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 21, 1113 Sofia, Bulgaria. vanya.bogoeva@gmail.com
authors.forename: Vanya P
authors.initials: VP
authors.lastname: Bogoeva
authors.forename: Lidiya P
authors.initials: LP
authors.lastname: Petrova
authors.forename: Ivan B
authors.initials: IB
authors.lastname: Ivanov
authors.forename: Hristina N
authors.initials: HN
authors.lastname: Kulina
authors.forename: Ivan Ch
authors.initials: ICh
authors.lastname: Buchvarov
mesh_terms: D000817:Anilino Naphthalenesulfonates; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D001665:Binding Sites; D002945:Cisplatin / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D005456:Fluorescent Dyes; D006046:Gold / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D008665:Metalloporphyrins / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism; D010984:Platinum / Q000737:chemistry; D011485:Protein Binding; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014909:Wheat Germ Agglutinins / Q000737:chemistry*
chemical_list: C515594:8-anilino-1-naphthalenesulfonic acid; D000817:Anilino Naphthalenesulfonates; D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; D005456:Fluorescent Dyes; D008665:Metalloporphyrins; D012996:Solutions; D014909:Wheat Germ Agglutinins; D010984:Platinum; D006046:Gold; D002945:Cisplatin