title: In vitro antiviral activity of Brazilian plants (Maytenus ilicifolia and Aniba rosaeodora) against bovine herpesvirus type 5 and avian metapneumovirus.
abstract: BACKGROUND
  Medicinal plants are well known for their use in traditional folk medicine as treatments for many diseases including infectious diseases.
  
  
  OBJECTIVE
  Six Brazilian medicinal plant species were subjected to an antiviral screening bioassay to investigate and evaluate their biological activities against five viruses: bovine herpesvirus type 5 (BHV-5), avian metapneumovirus (aMPV), murine hepatitis virus type 3, porcine parvovirus and bovine respiratory syncytial virus.
  
  
  METHODS
  The antiviral activity was determined by a titration technique that depends on the ability of plant extract dilutions (25 or 2.5 µg/mL) to inhibit the viral induced cytopathic effect and the extracts' inhibition percentage (IP).
  
  
  RESULTS
  Two medicinal plant species showed potential antiviral activity. The Aniba rosaeodora Ducke (Lauraceae) extract had the best results, with 90% inhibition of viral growth at 2.5 µg/mL when the extract was added during the replication period of the aMPV infection cycle. The Maytenus ilicifolia (Schrad.) Planch. (Celastraceae) extracts at a concentration of 2.5 µg/mL exhibited antiviral activity during the attachment phase of BHV-5 (IP = 100%).
  
  
  CONCLUSIONS
  The biomonitored fractionation of the active extracts from M. ilicifolia and A. rosaeodora could be a potential tool for identifying their active compounds and determining the exact mechanism of action.
authors.affiliation: Laboratory of Virology, Institute of Biology, University of Campinas – Unicamp, Campinas, São Paulo, Brazil.
authors.forename: L K
authors.initials: LK
authors.lastname: Kohn
authors.forename: C L
authors.initials: CL
authors.lastname: Queiroga
authors.forename: M C
authors.initials: MC
authors.lastname: Martini
authors.forename: L E
authors.initials: LE
authors.lastname: Barata
authors.forename: P S S
authors.initials: PS
authors.lastname: Porto
authors.forename: L
authors.initials: L
authors.lastname: Souza
authors.forename: C W
authors.initials: CW
authors.lastname: Arns
mesh_terms: D000820:Animal Diseases / Q000188:drug therapy / Q000821:virology; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D001938:Brazil; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D025881:Herpesvirus 5, Bovine / Q000187:drug effects; D019770:Lauraceae / Q000737:chemistry; D029755:Maytenus / Q000737:chemistry; D008519:Medicine, Traditional; D029121:Metapneumovirus / Q000187:drug effects; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D013552:Swine; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts