title: Identification of genes differentially expressed in grapevine associated with resistance to Elsinoe ampelina through suppressive subtraction hybridization.
abstract: Anthracnose, caused by the biotrophic fungus Elsinoe ampelina, is an economically devastating disease of grapevine (Vitis vinifera L.) prevalent in warm and humid regions of the world. In order to investigate the molecular resistance mechanisms and identify genes related to anthracnose resistance in grapevine, a Suppression Subtractive Hybridization (SSH) library was constructed using mixed cDNAs prepared from leaves of Chinese wild Vitis quinquangularis clone 'Shang-24', cDNA prepared from leaves infected with the pathogen E. ampelina served as tester and cDNA from mock-inoculated leaves as driver. A total of 670 high-quality ESTs were clustered and assembled into a collection of 461 unique genes comprising 85 contigs and 376 singletons. By Gene ontology (GO) analysis 310 unigenes were assigned to 22 GO slims within the molecular function category, while 317 unigenes could be sorted into 43 GO slims within the biological process category. The expression profiles of 20 selected genes, monitored by quantitative RT-PCR, indicated that expression of these genes in the E. ampelina-resistant 'Shang-24' was quicker and more intense, than in the susceptible 'Red Globe' where the reaction was delayed and limited. The results imply that these up-regulated genes could be involved in grapevine responses against E. ampelina infection.
authors.affiliation: College of Horticulture, Northwest A&F University, Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Yangling 712100, Shaanxi, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Ran
authors.initials: R
authors.lastname: Wan
authors.forename: Zhangjun
authors.initials: Z
authors.lastname: Fei
authors.forename: Xiping
authors.initials: X
authors.lastname: Wang
mesh_terms: D001203:Ascomycota; D018076:DNA, Complementary; D060467:Disease Resistance / Q000235:genetics*; D020224:Expressed Sequence Tags; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D009693:Nucleic Acid Hybridization / Q000379:methods; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics*; D018515:Plant Leaves; D015854:Up-Regulation; D027843:Vitis / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary