title: Regulation of gene expression with thyroid hormone in rats with myocardial infarction.
abstract: BACKGROUND
  The expression of hundreds of genes is altered in response to left ventricular (LV) remodeling following large transmural myocardial infarction (MI). Thyroid hormone (TH) improves LV remodeling and cardiac performance after MI. However, the molecular basis is unknown.
  
  
  METHODS
  MI was produced by ligation of the left anterior descending coronary artery in female SD rats. Rats were divided into the following groups: (1) Sham MI, (2) MI, and (3) MI+T4 treatment (T4 pellet 3.3 mg, 60 days release, implanted subcutaneously immediately following MI). Four weeks after surgery, total RNA was isolated from LV non-infarcted areas for microarray analysis using the Illumina RatRef-12 Expression BeadChip Platform.
  
  
  RESULTS
  Signals were detected in 13,188 genes (out of 22,523), of which the expression of 154 genes were decreased and the expression of 200 genes were increased in MI rats compared with Sham MI rats (false discovery rate (FDR) <0.05). Compared to MI rats, T4 treatment decreased expression of 27 genes and increased expression of 28 genes. In particular, 6 genes down-regulated by MI and 12 genes up-regulated by MI were reversed by T4. Most of the 55 genes altered by T4 treatment are in the category of molecular function under binding (24) and biological processes which includes immune system process (9), multi-organism process (5) and biological regulation (19) nonexclusively.
  
  
  CONCLUSIONS
  These results suggest that altered expression of genes for molecular function and biological process may be involved in the beneficial effects of thyroid hormone treatment following MI in rats.
authors.affiliation: Department of Biomedical Sciences, New York College of Osteopathic Medicine of New York Institute of Technology, Old Westbury, New York, United States of America. ychen33@nyit.edu
authors.forename: Yue-Feng
authors.initials: YF
authors.lastname: Chen
authors.forename: James V
authors.initials: JV
authors.lastname: Pottala
authors.forename: Nathan Y
authors.initials: NY
authors.lastname: Weltman
authors.forename: Xijin
authors.initials: X
authors.lastname: Ge
authors.forename: Olga V
authors.initials: OV
authors.lastname: Savinova
authors.forename: A Martin
authors.initials: AM
authors.lastname: Gerdes
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D009124:Muscle Proteins / Q000096:biosynthesis*; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013963:Thyroid Hormones / Q000378:metabolism*; D020257:Ventricular Remodeling
chemical_list: D009124:Muscle Proteins; D013963:Thyroid Hormones