title: Using ES cells labeled with GFP for analyzing cell behavior during differentiation.
abstract: Mouse embryonic stem (ES) cell is a unique yet highly successful system to study stem cell maintenance and differentiation. In this protocol, we describe the generation of engineered mouse ES cells that stably express GFP alone or GFP fused with histone H2B, which allow the cells to be traced live or in fixed samples. This system can be used to study cell behavior changes as ES cells differentiate into different lineages.
authors.affiliation: Carnegie Institution for Science, Department of Embryology, Baltimore, Maryland, USA.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
authors.forename: Queenie P
authors.initials: QP
authors.lastname: Vong
authors.forename: Yixian
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D003114:Colony-Forming Units Assay; D004475:Ectoderm / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D051379:Mice; D013194:Staining and Labeling / Q000379:methods*; D059008:Time-Lapse Imaging
chemical_list: D006657:Histones; D049452:Green Fluorescent Proteins