title: Hematopoietic effect of Bacillus subtilis-fermented antler extract on phenylhydrazine-induced hemolytic anemia in Sprague-Dawley rats.
abstract: This study examined the effect of fermentation on the ability of antler to act as a stimulator of hematopoietic activity. Hemolytic anemia was induced by phenylhydrazine (PHZ) in female Sprague-Dawley rats. The vehicle or antler extract (nonfermented or fermented) mixed in drinking water was administered from Days 2 to 15 after PHZ injection. On Day 15, red blood cell counts in the fermented antler group (6.33×10⁶/μL) were significantly higher than those in the nonfermented antler group (5.90×10⁶/μL) (P<.05), and rats treated with fermented antler extract tended to have higher hemoglobin compared with rats treated with nonfermented antler extract, but not significantly. In addition, rats treated with fermented antler extract had slightly lower serum erythropoietin levels compared with nonfermented antler extract, which were not statistically different from serum erythropoietin levels of nonanemic rats. We conclude therefore that the hematopoietic activity of antler might be increased by the fermentation process.
authors.affiliation: Kwang Dong Pharmaceutical Co., Ltd., Gyeonggi-do, Korea.
authors.forename: Sang Hun
authors.initials: SH
authors.lastname: Lee
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
authors.forename: Hyun-Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
authors.forename: Jang-Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Jung
mesh_terms: D000743:Anemia, Hemolytic / Q000097:blood / Q000178:diet therapy*; D000818:Animals; D000999:Antlers / Q000737:chemistry*; D001412:Bacillus subtilis / Q000378:metabolism*; D003670:Deer; D004906:Erythrocyte Count; D004921:Erythropoietin / Q000097:blood; D005260:Female; D005285:Fermentation; D057141:Food, Preserved / Q000009:adverse effects / Q000382:microbiology*; D006397:Hematinics / Q000009:adverse effects / Q000378:metabolism / Q000627:therapeutic use*; D006410:Hematopoiesis; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008297:Male; D008419:Materia Medica / Q000009:adverse effects / Q000378:metabolism / Q000627:therapeutic use*; D008518:Medicine, East Asian Traditional; D009996:Osmotic Fragility; D010659:Phenylhydrazines; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006397:Hematinics; D006454:Hemoglobins; D008419:Materia Medica; D010659:Phenylhydrazines; C030299:phenylhydrazine; D004921:Erythropoietin