title: Optimized PEF treatment for antioxidant polypeptides with MW 10-30 kDa and preliminary analysis of structure change.
abstract: Antioxidant polypeptides of molecular weight (MW) ranging from 10 to 30 kDa were produced from egg-white protein powder by enzyme hydrolysis and ultrafiltration (UF). Ferric reducing antioxidant potential (FRAP) value (mmol Fe(2+)/g) was used to evaluate the antioxidant activity. One-factor-at-a-time (OFAT) tests and Box-Behnken design (BBD) of response surface methodology (RSM) were used to investigate the effect of pulsed electric field (PEF) treatment parameters on antioxidant activity of polypeptides. The optimal conditions were as follows: concentration 8 mg/mL, electric field intensity 10 kV/cm, and pulse frequency 2000 Hz, under which, the FRAP value increased 44.23%, compared to the antioxidant activity of the polypeptides without PEF treatment. Both near-infrared spectroscopy (NIR) and mid-infrared spectroscopy (MIR) were used to analyze the change of functional groups.
authors.affiliation: Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Songyi
authors.initials: S
authors.lastname: Lin
authors.forename: Bolong
authors.initials: B
authors.lastname: Liu
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
authors.forename: Gregory
authors.initials: G
authors.lastname: Jones
authors.forename: Hsiang-Chi
authors.initials: HC
authors.lastname: Huang
mesh_terms: D004527:Egg Proteins / Q000737:chemistry; D004560:Electricity; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology*; D007501:Iron / Q000737:chemistry; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D004527:Egg Proteins; D016166:Free Radical Scavengers; D010446:Peptide Fragments; D007501:Iron