title: [MicroRNAs expression in normal and dissected aortic tissue].
abstract: OBJECTIVE
  To investigate if there is altered microRNAs (miRNAs) expression in aortic dissection (Debakey Type A) and normal aorta tissue.
  
  
  METHODS
  Total RNA was exacted from aorta of 5 patients with aortic dissection (AD) and four patients without aortic diseases (NA). miRNAs of the aortic tissues were analyzed by miRNA microarray. Reverse transcription polymerase chain reaction (RT-PCR) was performed to verify the expression of miRNAs in larger sample size (AD = 11 and NA = 9).
  
  
  RESULTS
  hsa-miR-146b-5p_st, hsa-miR-19a_st and hsa-miR-505_st were significantly upregulated while hsa-miR-1268_st and hsa-miR-939_st were significantly downregulated [fold change > 2, q-value (%) ≤ 5] in AD group compared with NA group. RT-PCR verified hsa-miR-146b-5p_st miRNAs change in AD group.
  
  
  CONCLUSIONS
  Altered miRNAs expression might play an essential role in the pathogenesis of aortic dissection formation and hsa-miR-146b-5p_st might serve as a new diagnosis biomarker of aortic dissection.
authors.affiliation: Guangdong Academy of Medical Sciences, Guangdong General Hospital, Guangzhou, China.
authors.forename: Zi-yang
authors.initials: ZY
authors.lastname: Hu
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: Luo
authors.forename: Shi-long
authors.initials: SL
authors.lastname: Zhong
authors.forename: Ling
authors.initials: L
authors.lastname: Xue
authors.forename: Yi-fei
authors.initials: YF
authors.lastname: Chen
authors.forename: Rui-xin
authors.initials: RX
authors.lastname: Fan
mesh_terms: D000784:Aortic Dissection / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs