title: Hormonal differences in peripheral blood and gene profiling in the liver and lymphocytes in Japanese black cattle with growth retardation.
abstract: Japanese Black cattle occasionally demonstrate growth retardation despite sufficient nutrient intake. To clarify hormonal and transcriptional characteristics, we investigated differences in blood components, including hormones, and differences in exhaustive gene expressions in the liver and peripheral lymphocytes of six cattle with growth retardation (GR cattle) and eight control cattle of the same age and pedigree with normal growth. Hematocrit values and concentrations of hemoglobin, serum albumin, total cholesterol, insulin-like growth factor 1 (IGF-1), thyroxine and insulin in GR cattle were significantly lower than those in controls. GR cattle also excreted higher levels of GH. We used three GR and three control cattle for a microarray analysis in the liver and found that 279 gene expressions were significantly different. However, gene expressions related to the GH-IGF-1 axis, such as the GH receptor and IGF-1, were not significantly different from those of controls. Immune-related gene expressions were significantly lower. To clarify these gene expression levels, peripheral lymphocytes were used for real-time RT-PCR. The expression rates of genes that were significantly lower in the liver, such as chemokine ligand 8, interferon gamma receptor 1 and immunoglobulin light chain VJ region were also significantly lower in three GR cattle than those in the three control cattle. These results suggest that the cause of growth retardation in the present study was due to other factors, not abnormal gene expressions of factors related to the GH-IGF-1 axis in the liver, and that GR cattle were susceptible to infectious disease.
authors.affiliation: Laboratory of Large Animal Surgery, School of Veterinary Medicine, Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, Japan.
authors.forename: Sakura
authors.initials: S
authors.lastname: Ishida
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yonezawa
authors.forename: Sayoko
authors.initials: S
authors.lastname: Eirai
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Kizaki
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hashizume
authors.forename: Michiko
authors.initials: M
authors.lastname: Tomioka
authors.forename: Yohei
authors.initials: Y
authors.lastname: Kurose
authors.forename: Takashi
authors.initials: T
authors.lastname: Hirano
authors.forename: Daisaku
authors.initials: D
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006130:Growth Disorders / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D006400:Hematocrit / Q000662:veterinary; D006728:Hormones / Q000097:blood*; D007147:Immunoglobulin Light Chains / Q000378:metabolism; D007564:Japan; D008099:Liver / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D046228:Microarray Analysis / Q000662:veterinary; D010375:Pedigree; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017471:Receptors, Interferon / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D000096743:Interferon gamma Receptor
chemical_list: D017931:DNA Primers; D006728:Hormones; D007147:Immunoglobulin Light Chains; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor