title: Xanthohumol prevents carbon tetrachloride-induced acute liver injury in rats.
abstract: Xanthohumol (XN), a prenyl flavonoid present in beer, prevents the acute hepatic injury induced by carbon tetrachloride (CCl4) in rats. Pre-treatment of rats with XN significantly reduced the increased liver weight observed in CCl4-intoxicated rats, normalised the increased values of plasma lactate dehydrogenase, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase activities and reduced the incidence of histopathological alterations produced by CCl4. The oxidative stress induced by CCl4 administration elicited a significant decrease in the levels of reduced glutathione as well as an increase in thiobarbituric acid reactive substances (TBARS) and H2O2 concentrations. Pre-treatment of rats with XN resulted in a significant (p<0.05) increase in reduced glutathione (GSH) content and a reduction in TBARS and H2O2 concentrations to their normal values. XN pre-treatment also prevented the significant reductions of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase activities observed in CCl4-treated rats compared to control animals. Our results suggest that the hepatoprotective effect of XN is based on its antioxidant properties as well as it being an efficient inhibitor of lipid peroxidation and a protector against the degradation of antioxidant enzymes induced by CCl4 intoxication.
authors.affiliation: Department of Biochemistry and Molecular Biology, Science Faculty, Extremadura University, Av. Elvas s/n, 06006 Badajoz, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Pinto
authors.forename: Antonio L
authors.initials: AL
authors.lastname: Duque
authors.forename: Beatriz
authors.initials: B
authors.lastname: Rodríguez-Galdón
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Cestero
authors.forename: Pedro
authors.initials: P
authors.lastname: Macías
mesh_terms: D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000473:pathology; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000473:pathology; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000473:pathology; D015394:Molecular Structure; D016877:Oxidants / Q000378:metabolism; D011427:Propiophenones / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005419:Flavonoids; D016877:Oxidants; D011427:Propiophenones; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; C104536:xanthohumol