title: The effect of vitamin D on expression of TGF β1 in ovary.
abstract: The transforming growth factor β (TGF-β) is thought to have important roles in several stages of folliculogenesis. Vitamin D is effective in cell proliferation, differentition and on estrogen biosynthesis. The aim of the present study was to determine the respective role of 1,25-dihydroxyvitamin D3 on expression of TGF-β1 in developing rat ovaries. 24 (one-mounth-old n = 12 and adult n = 12) female Wistar rats were enrolled in this study. All animals were divided into 4 groups. Group I and II that consist of one-month-old (n = 6) and adult rats (n = 6) respectively served as control groups. Intramuscular vitamin D3 ( 0,05 μgr/kg/every other day) was injected for 8 weeks to group III and IV which consist of one-mounth-old and adult rats respectively. After last injection, ovaries of animals were removed and processed for immunohistochemistry assay. No remarkable differences in staining intensity and localization for TGF-β1 were observed in group I and group III. TGF immunostaining was also predominantly found in oocytes. In granulosa cells, TGF-β1 immunoreactivity was negative. TGF-β1 immunostaining were observed both in nuclei and cytoplasm of granulosa cells in group II. But in group IV granulosa cells and oocytes were negative for TGF-β1. We found that vitamin D administration resulted in a decrease in TGF-β1 levels in the adult rats, but, TGF-β1 expression did not significantly decrease in the newborn rats. However, in multiple linear regression analysis, TGF-β1 expressions were independently associated with vitamin D administration. It is observed that Vitamin D attenuated TGF-β1 expression. The results of this study suggest that vitamin D may play role in folliculogenesis via TGF-β1.
authors.affiliation: Pediatric Surgery, Pamukkale University School of Medicine, Denizli, Turkey. ncorduk@pau.edu.tr
authors.forename: N
authors.initials: N
authors.lastname: Corduk
authors.forename: G
authors.initials: G
authors.lastname: Abban
authors.forename: B
authors.initials: B
authors.lastname: Yildirim
authors.forename: A
authors.initials: A
authors.lastname: Sarioglu-Buke
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002762:Cholecalciferol / Q000008:administration & dosage / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D007273:Injections, Intramuscular; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D009866:Oogenesis; D009928:Organ Specificity; D010053:Ovary / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D046468:Sexual Development; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D002762:Cholecalciferol; D002117:Calcitriol