title: Pancreatic-specific autoantibodies to glycoprotein 2 mirror disease location and behaviour in younger patients with Crohn's disease.
abstract: BACKGROUND
  Glycoprotein 2 (GP2) was discovered as the major autoantigen of Crohn's disease (CD)-specific pancreatic autoantibodies (PAB). We investigated anti-GP2 IgA and IgG antibodies as novel serological parameters in CD and assessed their association with distinct disease phenotypes.
  
  
  METHODS
  Anti-GP2 and anti-Saccharomyces cerevisiae (ASCA) IgA and IgG were detected by ELISA employing recombinant human GP2 and phosphopeptidomannan, respectively and PAB by indirect immunofluorescence (IIF) in 271 sera, 169 with CD and 102 with ulcerative colitis (UC). As healthy controls 160 adult blood donors and 65 children were included.
  
  
  RESULTS
  Anti-GP2 IgG and/or IgA were more prevalent in CD (51/169, 30.2%) than in UC (9/102, 8.9%) patients and in controls (9/225, 4%) (p < 0.001 respectively). ASCA IgG and/or IgA were present in 60/169 (35.5%) in CD and in 7/102 (6.9%) in UC patients (p < 0.001). CD patients with ileocolonic location (L3) showed a significantly higher prevalence of anti-GP2 and ASCA IgA and/or IgG (40/113 and 48/113, respectively; p < 0.05 for both comparisons), whereas CD patients with colonic location (L2) revealed a significantly diminished prevalence for these autoantibody specificities (2/32 and 5/32, respectively, p < 0.05 for both). Anti-GP2 IgG were significantly more prevalent in CD patients with stricturing behaviour (B2) and perianal disease (7/11, p < 0.02) and less prevalent in those with penetrating behaviour (B3) and perianal disease (4/31, p < 0.05). The occurrence of anti-GP2 IgA and/or IgG was significantly more prevalent in CD patients with age at diagnosis of ≤16 years (16/31, p < 0.009). Prevalence of one or more anti-GP2 or ASCA IgA and/or IgG was significantly higher in L3, B2, and A1 and lower in L2 (68/113, 27/41, 23/31, 6/32; p < 0.04, respectively).
  
  
  CONCLUSIONS
  Anti-GP2 IgG and IgA, constituting novel CD specific autoantibodies, appear to be associated with distinct disease phenotypes identifying patients at a younger age, with ileocolonic location, and stricturing behaviour with perianal disease.
authors.affiliation: Division of Transplantation Immunology and Mucosal Biology, King's College London School of Medicine at King’s College Hospital, Denmark Hill Campus, Bessemer Road, London SE5 9RJ, UK.
authors.forename: Dimitrios P
authors.initials: DP
authors.lastname: Bogdanos
authors.forename: Dirk
authors.initials: D
authors.lastname: Roggenbuck
authors.forename: Dirk
authors.initials: D
authors.lastname: Reinhold
authors.forename: Thomas
authors.initials: T
authors.lastname: Wex
authors.forename: Polychronis
authors.initials: P
authors.lastname: Pavlidis
authors.forename: Ulrike
authors.initials: U
authors.lastname: von Arnim
authors.forename: Peter
authors.initials: P
authors.lastname: Malfertheiner
authors.forename: Alastair
authors.initials: A
authors.lastname: Forbes
authors.forename: Karsten
authors.initials: K
authors.lastname: Conrad
authors.forename: Martin W
authors.initials: MW
authors.lastname: Laass
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D002648:Child; D003093:Colitis, Ulcerative / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology; D003108:Colonic Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D003424:Crohn Disease / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology*; D005260:Female; D058851:GPI-Linked Proteins / Q000276:immunology*; D006801:Humans; D007077:Ileal Diseases / Q000097:blood / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000276:immunology*; D015995:Prevalence; D012441:Saccharomyces cerevisiae / Q000276:immunology; D055815:Young Adult
chemical_list: D001323:Autoantibodies; C489339:GP2 protein, human; D058851:GPI-Linked Proteins