title: Comparative biochemical properties of vertebrate deoxyribonuclease I.
abstract: Deoxyribonuclease I (DNase I, EC 3.1.21.1) is an endonuclease that preferentially attacks double-stranded DNA in a Ca(2+)-dependent manner to produce oligonucleotides with 5'-phospho and 3'-hydroxy termini. This review deals with the biochemical properties and molecular evolution of DNase I. A comparative study of vertebrate DNase I from Chondrichthyes to Homo sapiens has been carried out. The optimal pH, the role of N-glycosylation, actin inhibition, thermal stability, pH stability, and structure stability are discussed. Moreover, a phylogenetic analysis was performed. The levels of DNase I activity in serum have been suggested to be a critical factor in the initiation of human and mouse SLE. Moreover, as shown above, DNase I is utilized in the treatment of patients with cystic fibrosis. Our comparative study of the biochemical properties and molecular analysis of DNase I will be helpful in the use of DNase I for clinical use.
authors.affiliation: Department of Legal Medicine, Shimane University School of Medicine, Shimane, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Fujihara
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Yasuda
authors.forename: Misuzu
authors.initials: M
authors.lastname: Ueki
authors.forename: Reiko
authors.initials: R
authors.lastname: Iida
authors.forename: Haruo
authors.initials: H
authors.lastname: Takeshita
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003850:Deoxyribonuclease I / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D019143:Evolution, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D014714:Vertebrates
chemical_list: D003850:Deoxyribonuclease I