title: Porcine CD27: identification, expression and functional aspects in lymphocyte subsets in swine.
abstract: Up to now for Swine Workshop Cluster 2 (SWC2) the orthologous human CD molecule was unknown. By use of the SWC2-specific mAb b30c7 and a retroviral cDNA expression library derived from stimulated porcine peripheral blood mononuclear cells we could identify SWC2 as porcine CD27. Phenotypic analyses of lymphocytes isolated from blood and lymphatic organs revealed that mature T cells in thymus and T cells in the periphery with a naïve phenotype were CD27(+). However, within CD8α(+) T helper and CD8α(+) γδ T cells also CD27(-) cells were present, indicating a down-regulation after antigen contact in vivo. B cells lacked CD27 expression, whereas NK cells expressed intermediate levels. Furthermore, plate-bound mAb b30c7 showed a costimulatory capacity on CD3-activated T cells for proliferation, IFN-γ and TNF-α production. Hence, our data indicate an important role of porcine CD27 for T-cell differentiation and activation as described for humans and mice.
authors.affiliation: Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Reutner
authors.forename: Judith
authors.initials: J
authors.lastname: Leitner
authors.forename: Sabine E
authors.initials: SE
authors.lastname: Essler
authors.forename: Kirsti
authors.initials: K
authors.lastname: Witter
authors.forename: Martina
authors.initials: M
authors.lastname: Patzl
authors.forename: Peter
authors.initials: P
authors.lastname: Steinberger
authors.forename: Armin
authors.initials: A
authors.lastname: Saalmüller
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Gerner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7