title: Mouse Hobit is a homolog of the transcriptional repressor Blimp-1 that regulates NKT cell effector differentiation.
abstract: The transcriptional repressor Blimp-1 mediates the terminal differentiation of many cell types, including T cells. Here we identified Hobit (Znf683) as a previously unrecognized homolog of Blimp-1 that was specifically expressed in mouse natural killer T cells (NKT cells). Through studies of Hobit-deficient mice, we found that Hobit was essential for the formation of mature thymic NKT cells. In the periphery, Hobit repressed the accumulation of interferon-γ (IFN-γ)-producing NK1.1(lo) NKT cells at steady state. After antigenic stimulation, Hobit repressed IFN-γ expression, whereas after innate stimulation, Hobit induced granzyme B expression. Thus, reminiscent of the function of Blimp-1 in other lymphocytes, Hobit controlled the maintenance of quiescent, fully differentiated NKT cells and regulated their immediate effector functions.
authors.affiliation: Department of Hematopoiesis, Sanquin Research at CLB and Landsteiner Laboratory, Amsterdam, The Netherlands. k.vangisbergen@sanquin.nl
authors.forename: Klaas P J M
authors.initials: KP
authors.lastname: van Gisbergen
authors.forename: Natasja A M
authors.initials: NA
authors.lastname: Kragten
authors.forename: Kirsten M L
authors.initials: KM
authors.lastname: Hertoghs
authors.forename: Felix M
authors.initials: FM
authors.lastname: Wensveen
authors.forename: Stipan
authors.initials: S
authors.lastname: Jonjic
authors.forename: Jörg
authors.initials: J
authors.lastname: Hamann
authors.forename: Martijn A
authors.initials: MA
authors.lastname: Nolte
authors.forename: Rene A W
authors.initials: RA
authors.lastname: van Lier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D005434:Flow Cytometry; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D055611:Natural Killer T-Cells / Q000166:cytology* / Q000276:immunology*; D010802:Phylogeny; D000074462:Positive Regulatory Domain I-Binding Factor 1; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics
chemical_list: C513258:Prdm1 protein, mouse; D014157:Transcription Factors; D000074462:Positive Regulatory Domain I-Binding Factor 1