title: Outer membrane vesicles (OMV) production of Neisseria meningitidis serogroup B in batch process.
abstract: Serogroup B outer membrane vesicles (OMV) with iron regulated proteins (IRP) from Neisseria meningitidis constitute the antigen for the vaccine against the disease caused by this bacterium. Aiming to enhance final OMV concentration, seven batch experiments were carried out under four different conditions: (i) with original Catlin medium; (ii) with original Catlin medium and lactate and amino acids pulse at the 6th cultivation hour; (iii) with Catlin medium with double initial concentrations of lactate and amino acids and (iv) Catlin medium without glycerol and with double initial concentrations of lactate and amino acids. The cultivation experiments were carried out in a 7-L bioreactor under the following conditions: 36°C, 0.5atm, overlay air 1L/min, agitation: 250-850 rpm, and O(2) control at 10%, 20 h. After lactate and amino acids exhaustion, cell growth reached stationary phase and a significant release increase of OMV was observed. According to the Luedeking & Piret model, OMV liberation is non-growth associated. Glycerol was not consumed during cultivation. The maximum OMV concentration value attained was 162 mg/L with correspondent productivity of 8.1mg/(Lh) employing Catlin medium with double initial concentrations of lactate and amino acids. The obtained OMV satisfied constitution and protein pattern criteria and were suitable for vaccine production.
authors.affiliation: Faculdade Adventista da Bahia (Northeast Brazil College), BR-101, km 197 - Capoeiruçu, Caixa Postal 18, Cachoeira, Bahia CEP 44300-000, Brazil. lemassilvia@yahoo.com.br
authors.forename: Sílvia
authors.initials: S
authors.lastname: Santos
authors.forename: Luciana Juncioni de
authors.initials: LJ
authors.lastname: Arauz
authors.forename: Júlia
authors.initials: J
authors.lastname: Baruque-Ramos
authors.forename: Ivo
authors.initials: I
authors.lastname: Lebrun
authors.forename: Sylvia Mendes
authors.initials: SM
authors.lastname: Carneiro
authors.forename: Sandra Alves
authors.initials: SA
authors.lastname: Barreto
authors.forename: Rocilda Perazzini Furtado
authors.initials: RP
authors.lastname: Schenkman
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry; D005990:Glycerol / Q000378:metabolism; D035925:Iron-Regulatory Proteins / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry; D022401:Meningococcal Vaccines / Q000096:biosynthesis; D038541:Neisseria meningitidis, Serogroup B / Q000378:metabolism*; D022142:Secretory Vesicles / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D035925:Iron-Regulatory Proteins; D022401:Meningococcal Vaccines; D019344:Lactic Acid; D005990:Glycerol