title: Casein modified gold nanoparticles for future theranostic applications.
abstract: The synthesis and characterization of gold nanoparticles (AuNPs 20 nm sized) modified with k-casein derived peptides in order to monitor the peptide effect as bacterial adhesion inhibitor, thanks to the carrier/concentrator effect of the AuNPs is here presented. Some aspects related to the stability of AuNP/peptide conjugates for a potential application in the design of an electrochemical biosensor for pathogen bacteria detection are also discussed. This peptide based nanoparticle assay takes advantage of the dual character of the AuNPs: as carrier of the biorecognition molecule and also as electrocatalytic label, allowing the evaluation of the pathogen bacteria-peptide interaction in a simple and rapid way through the chronoamperometric monitoring of the hydrogen evolution reaction on screen-printed carbon electrodes. The developed proof of concept theranostic system may open the way to therapeutic and biosensing applications with interest for various fields.
authors.affiliation: Nanobioelectronics & Biosensors Group, CIN2 (ICN-CSIC), Catalan Institute of Nanotechnology, Campus UAB, Bellaterra (Barcelona), Spain.
authors.forename: Marisol
authors.initials: M
authors.lastname: Espinoza-Castañeda
authors.forename: Alfredo
authors.initials: A
authors.lastname: de la Escosura-Muñiz
authors.forename: Gemma
authors.initials: G
authors.lastname: González-Ortiz
authors.forename: Susana M
authors.initials: SM
authors.lastname: Martín-Orúe
authors.forename: José Francisco
authors.initials: JF
authors.lastname: Pérez
authors.forename: Arben
authors.initials: A
authors.lastname: Merkoçi
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002364:Caseins / Q000008:administration & dosage* / Q000627:therapeutic use; D054307:Enterotoxigenic Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000627:therapeutic use; D053768:Metal Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D002364:Caseins; D006046:Gold