title: Isolation and  molecular characterization of a novel strain of Bacillus with antifungal  activity from the sorghum rhizosphere.
abstract: We  looked for bacterial strains with antifungal activity in the sorghum  rhizosphere. A prescreening procedure to search for hemolytic activity among  the isolated strains allowed us to detect good fungitoxic activity in a  bacterial isolate that we named UM96. This bacterial isolate showed strong  growth inhibition in bioassays against the pathogens Diaporthe phaseolorum, Colletotrichum acutatum, Rhizoctonia solani, and Fusarium  oxysporum. The supernatant of isolate UM96 also showed strong hemolytic  activity, which was not observed in the protease-treated supernatant. However,  the supernatant that was treated with protease had similar antagonistic effects  to those exhibited by the supernatant that was not treated with this enzyme.  These results suggest that a bacteriocin-like compound is responsible for the  hemolytic activity; whereas, as far as antifungal effect is concerned, an  antibiotic of nonribosomal origin, such as a lipopeptide, might be acting.  Further molecular characterization by partial 16S rDNA sequencing placed  isolate UM96 in a cluster with Bacillus amyloliquefaciens; however, the  highest identity match found in databases of Bacillus species was 91%  identity. This suggests that Bacillus sp UM96 might be a novel species.
authors.affiliation: Instituto de Investigaciones Quimico-Biologicas, Universidad Michoacana de San Nicolas de Hidalgo, Morelia, Michoacan, Mexico.
authors.forename: S C
authors.initials: SC
authors.lastname: Martinez-Absalon
authors.forename: Ma
authors.initials: M
authors.lastname: del C Orozco-Mosqueda
authors.forename: M M
authors.initials: MM
authors.lastname: Martinez-Pacheco
authors.forename: R
authors.initials: R
authors.lastname: Farias-Rodriguez
authors.forename: M
authors.initials: M
authors.lastname: Govindappa
authors.forename: G
authors.initials: G
authors.lastname: Santoyo
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001407:Bacillus / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics; D005658:Fungi / Q000187:drug effects* / Q000254:growth & development; D005798:Genes, Bacterial / Q000235:genetics; D006461:Hemolysis / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D058441:Rhizosphere; D045868:Sorghum / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents; D004275:DNA, Ribosomal