title: Synthesis, spectroscopic characterization, antimicrobial, DNA binding and oxidative-induced DNA cleavage activities: new oxovanadium(IV) complexes of 2-(2-hydroxybenzylideneamino)isoindoline-1,3-dione.
abstract: A mononuclear complex [(phen)VO(bid)]SO(4)·3H(2)O (1), (phen=1,10-phenanthroline) and a binuclear [(VObid)(2)]·H(2)O (2), with Hbid [(E)-2-(2-hydroxybenzylideneamino)isoindoline-1,3-dione] were prepared and characterized by elemental analysis, IR, mass, UV-Vis spectral studies, ESR, molar conductance and thermogravimetric analysis. The DNA-binding properties of the complexes 1 and 2 were investigated by UV-spectroscopy, fluorescence spectroscopy and viscosity measurements. The superoxide dismutase-like activity of the complexes has been determined. The spectral results suggest that the complexes 1 and 2 can bind to DNA by intercalation. The cleavage properties of these complexes with super coiled pUC19 have been studied using the gel electrophoresis method, where both complexes 1 and 2 displayed chemical nuclease activity in the absence and presence of H(2)O(2)via an oxidative mechanism. All the complexes inhibit the growth of both Gram positive and Gram negative bacteria to competent level. Minimum inhibitory concentration (MIC) was determined by microtiter method.
authors.affiliation: Department of Chemistry, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India.
authors.forename: Linganna
authors.initials: L
authors.lastname: Shivakumar
authors.forename: Kollur
authors.initials: K
authors.lastname: Shivaprasad
authors.forename: Hosakere Dodda
authors.initials: HD
authors.lastname: Revanasiddappa
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects*; D004278:DNA, Superhelical / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D004996:Ethidium / Q000378:metabolism; D005658:Fungi / Q000187:drug effects; D054833:Isoindoles / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D060328:Magnetic Phenomena; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction / Q000187:drug effects; D012996:Solutions; D013055:Spectrophotometry, Infrared; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature; D013818:Thermogravimetry; D014638:Vanadates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D014783:Viscosity / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D004278:DNA, Superhelical; D054833:Isoindoles; D012996:Solutions; C075743:oxovanadium IV; D014638:Vanadates; D004247:DNA; D013482:Superoxide Dismutase; D004996:Ethidium; D004121:Dimethyl Sulfoxide