title: [Effect of catalpol and puerarin freeze-dried powder on coagulability, hemorheology and no in rats with Qi-deficiency and blood-stasis syndrome].
abstract: OBJECTIVE
  To study the effect of catalpol and puerarin freeze-dried powder for injection (CPFPI), a new compound traditional Chinese medicine (TCM) preparation, on coagulability, hemorheology and NO in rats with qi-deficiency and blood-stasis syndrome.
  
  
  METHODS
  The model of rats with qi-deficiency and blood-stasis syndrome was established by hunger, fatigue, cold-dampness, panic and high fat diet. Coagulation time (CT) was observed by the glass method, and bleeding time (BT) was measured by tail-cutting method. The effects of CPFPI were also evaluated with prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT). HCT was measured by the electric tesistance method, hemorheology indicators were observed by auto-hemorheological instrument. The level of NO in blood serum was measured by NO assay kit.
  
  
  RESULTS
  CPFPI 65.40 mg x kg(-1) significantly prolonged CT, BT, PT, APTT and TT in rats. The viscosity of whole blood and plasma, hematocrit, erythrocyte aggregation and rigidity index, and reduced viscosity of whole blood in 65.40 mg x kg(-1) groups were lower than model group. CPFPI 65.40 mg x kg(-1) can raise the level of NO in blood serum. 32.70 mg x kg(-1) markedly prolonged CT, PT and APTT and decreased whole blood viscosity, erythrocyte aggregation index and whole blood reduction viscosity.
  
  
  CONCLUSIONS
  CPFPI has a significant effect in improving coagulability and hemorheology index and enhancing NO content in blood serum.
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Chongqing Engineering Research Center for Pharmacodynamics Evaluation, Chongqing 400716, China. denglijuan1203@sina.com
authors.forename: Lijuan
authors.initials: L
authors.lastname: Deng
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Yuan
authors.forename: Jialan
authors.initials: J
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Tang
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D001778:Blood Coagulation Disorders / Q000097:blood / Q000188:drug therapy*; D001809:Blood Viscosity / Q000187:drug effects*; D005612:Freeze Drying; D057888:Iridoid Glucosides / Q000494:pharmacology*; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D008516:Medicine, Chinese Traditional; D009569:Nitric Oxide / Q000097:blood*; D011208:Powders; D019413:Qi; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D057888:Iridoid Glucosides; D007529:Isoflavones; D011208:Powders; C078040:catalpol; D009569:Nitric Oxide; C033607:puerarin