title: Are epidemiologic cut-off values predictors of the in vivo efficacy of azoles in experimental aspergillosis?
abstract: We tried to correlate the in vitro activity and the in vivo efficacy of voriconazole (VRC) and posaconazole (POS) against Aspergillus flavus and Aspergillus niger. The in vitro susceptibility of a large set of isolates was determined by broth microdilution and disk diffusion methods, while the in vivo efficacy was assessed in a murine model of disseminated infection. For A. flavus, VRC showed efficacy even for strains with MICs above the epidemiologic cutoff value (ECV) (1 μg/mL). For A. niger, VRC was ineffective against those strains for which the MIC was 1 dilution below the corresponding ECV (2 μg/mL). POS showed efficacy against all the strains of both species included in the study, although isolates with MICs > ECV were not tested.
authors.affiliation: Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, 21.43201, Reus, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Calvo
authors.forename: F Javier
authors.initials: FJ
authors.lastname: Pastor
authors.forename: Deanna A
authors.initials: DA
authors.lastname: Sutton
authors.forename: Anette W
authors.initials: AW
authors.lastname: Fothergill
authors.forename: Michael G
authors.initials: MG
authors.lastname: Rinaldi
authors.forename: Valentina
authors.initials: V
authors.lastname: Salas
authors.forename: Josep
authors.initials: J
authors.lastname: Guarro
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001228:Aspergillosis / Q000188:drug therapy*; D001231:Aspergillus flavus / Q000187:drug effects*; D001234:Aspergillus niger / Q000187:drug effects*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D011743:Pyrimidines / Q000008:administration & dosage* / Q000494:pharmacology; D016896:Treatment Outcome; D014230:Triazoles / Q000008:administration & dosage* / Q000494:pharmacology; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D011743:Pyrimidines; D014230:Triazoles; C101425:posaconazole; D065819:Voriconazole