title: [Protective effects of SUR2B/Kir6.1 potassium channel opener natakalim against RAVECs injuries induced by hypoxia].
abstract: OBJECTIVE
  To investigate the protective effects of natakalim against rat aortic vascular endothelial cells (RAVECs) injuries induced by hypoxia and its mechanisms.
  
  
  METHODS
  Selecting RAVECs as a cell model injured by hypoxia, these RAVECs were divided into 5 groups: i.e. control group, hypoxia group, natakalim low, medium and high group. The cell survival rate was determined by MTT assay, con was measured using Griess Assay, RT-PCR was used to examine t he expression of intercellular adhesion molecule-1 (ICAM-1), vascular endothelial growth factor (VEGF), endothelin-1 (ET-1) mRNA in RAVEC.
  
  
  RESULTS
  Natakalim could reverse hypoxia-induced changes in endothelial cell function, including increased endothelial cell survival rate and level of NO concentration, significantly inhibited the hypoxia-induced endothelial ICAM-1, ET-1, VEGF mRNA expression levels increased.
  
  
  CONCLUSIONS
  Natakalim have protective effects on hypoxia-induced changes in endothelial cell function, increasing of permeation, excess expression of cell adhesion molecules.
authors.affiliation: Key Laboratory of Millitary Enviromental Medicime Institute of Health and Environmental Medicine, Academy of Millitary Medical Sciences, Tianjin, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.forename: Yan-qing
authors.initials: YQ
authors.lastname: Cao
authors.forename: Jing-dan
authors.initials: JD
authors.lastname: Zhang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Peng
authors.forename: Dan-feng
authors.initials: DF
authors.lastname: Yang
authors.forename: Jia-yin
authors.initials: JY
authors.lastname: Liu
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Shi
authors.forename: Yong-lin
authors.initials: YL
authors.lastname: Yang
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
mesh_terms: D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism; D015687:Cell Hypoxia; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism*; D019332:Endothelin-1 / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008297:Male; D011437:Propylamines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D057772:Vascular System Injuries / Q000378:metabolism*
chemical_list: D000498:Allyl Compounds; D019332:Endothelin-1; D011437:Propylamines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C556401:natakalim; C467498:vascular endothelial growth factor A, rat; D018799:Intercellular Adhesion Molecule-1