title: Characterization of a Spodoptera frugiperda multiple nucleopolyhedrovirus isolate that does not liquefy the integument of infected larvae.
abstract: Here we report the characterization of a Spodoptera frugiperda multiple nucleopolyhedrovirus isolate, named SfMNPV-6nd, that does not cause the liquefaction of the host integument. The sequencing of the chitinase A (v-chiA) gene from SfMNPV-6nd revealed that it had a frameshift mutation that greatly reduced size of the putative enzyme. In order to evaluate the suitability of SfMPNV-6nd as a biopesticide, this isolate was compared with the highly virulent SfMNPV-19. Our results showed that the LC(50) of the two isolates were not significantly different, but that SfMNPV-6nd took a longer period of time to kill second instar S. frugiperda than SfMNPV-19.
authors.affiliation: Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes, SP, Brazil.
authors.forename: Corina M
authors.initials: CM
authors.lastname: Vieira
authors.forename: Edmar S
authors.initials: ES
authors.lastname: Tuelher
authors.forename: Fernando H
authors.initials: FH
authors.lastname: Valicente
authors.forename: José Luiz Caldas
authors.initials: JL
authors.lastname: Wolff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D061046:Biological Control Agents; D002688:Chitinases / Q000737:chemistry / Q000235:genetics; D016368:Frameshift Mutation; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000201:enzymology / Q000302:isolation & purification / Q000472:pathogenicity*; D018411:Spodoptera / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D014774:Virulence / Q000235:genetics
chemical_list: D061046:Biological Control Agents; D014764:Viral Proteins; D002688:Chitinases