title: Expression of oxidative stress in metastatic retinoblastoma- a comparative study.
abstract: OBJECTIVE
  To compare oxidative stress between primary retinoblastoma and retinoblastoma with distant metastasis.
  
  
  METHODS
  Forty consecutive patients presented with primary retinoblastoma and the same number of patients presented with distant metastasis, attending the outpatient department of our hospital between August 2002 and April 2005. All the patients with retinoblastoma underwent a standard metastasis workup and were subsequently categorized into two groups (without metastasis and with metastasis).Venous blood samples were drawn from each patient. After proper centrifugation, serum was collected and antioxidant enzymes and reactive oxygen species (ROS) were assayed.
  
  
  METHODS
  Serum collected from the patients was subjected to biochemical assay of the antioxidant enzymes (superoxide dismutase, catalase and peroxidise) and ROS to determine any difference in enzyme activity between the two groups.
  
  
  RESULTS
  Antioxidant levels were found to be less in the metastasis group as compared to the primary intraocular retinoblastoma group(p less than 0.05).Mean ROS activity was found to be increased in metastatic group (p less than 0.05).
  
  
  CONCLUSIONS
  The decreased antioxidant enzymes level along with increased ROS activity in patients with metastatic retinoblastoma reflect increased oxidative stress as compared to primary intraocular retinoblastoma patients.
authors.affiliation: Regional Institute of Ophthalmology, Kolkata, India. dr_somnath@hotmail.com
authors.forename: S
authors.initials: S
authors.lastname: Mukhopadhyay
authors.forename: J
authors.initials: J
authors.lastname: Dutta
authors.forename: R
authors.initials: R
authors.lastname: Raut
authors.forename: H
authors.initials: H
authors.lastname: Datta
authors.forename: A K
authors.initials: AK
authors.lastname: Bhattacharyay
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D002374:Catalase / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D009195:Peroxidase / Q000097:blood*; D017382:Reactive Oxygen Species / Q000097:blood*; D019572:Retinal Neoplasms / Q000097:blood* / Q000473:pathology; D012175:Retinoblastoma / Q000097:blood* / Q000556:secondary; D012189:Retrospective Studies; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D014408:Biomarkers, Tumor; D017382:Reactive Oxygen Species; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase