title: A rat model for stable chronic obstructive pulmonary disease induced by cigarette smoke inhalation and repetitive bacterial infection.
abstract: To develop a stable chronic obstructive pulmonary disease (COPD) model in rats. Sprague-Dawley rats were treated with cigarette-smoke inhalation (CSI) for 12 weeks, repetitive bacterial infection (RBI) for 8 weeks, or the combination of the two (CCR) for 12 weeks and followed up for the additional 20 weeks. Tidal volume (V(T)), peak expiratory flow (PEF) and 50% V(T) expiratory flow (EF(50)), histological changes in the lungs, and levels of the cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-8, and IL-10 in serum and bronchial alveolar lavage fluid (BALF) were examined at intervals during the 32 week study period. The right ventricular hypertrophy index (RVHI) was also determined at the same times. V(T), PEF, and EF(50) were decreased in rats with COPD compared to the control. The expression of TNF-α, IL-8 and IL-10 increased in both serum and BALF with a similar trend. Bronchiole and arteriole wall thickness and the degree of bronchiole stenosis and alveolar size increased in COPD rats. RVHI was reduced gradually following the treatment. All of these changes were more pronounced in the CCR-treatment group than in the other groups. Our results have shown that CSI or RBI alone can induce COPD in rats, but that the combination of CSI with RBI induces a stable COPD that has more similarity to complications seen in patients with COPD. This combination may therefore provide a more appropriate model for study of human COPD.
authors.affiliation: Institute of Respiratory Disease and Centre Laboratory, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou City, Henan Province 450008, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.forename: Su-Yun
authors.initials: SY
authors.lastname: Li
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.forename: Yan-Ge
authors.initials: YG
authors.lastname: Tian
authors.forename: Su-Li
authors.initials: SL
authors.lastname: Jiang
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wang
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007710:Klebsiella Infections / Q000150:complications* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D007711:Klebsiella pneumoniae; D008297:Male; D029424:Pulmonary Disease, Chronic Obstructive / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012129:Respiratory Function Tests; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana
chemical_list: D016207:Cytokines; D012906:Smoke