title: [Characteristics of histone deacetylase 9 in peripheral blood of patients with bronchial asthma].
abstract: OBJECTIVE
  To investigate the expression pattern of histone deacetylase 9 (HDAC9) in peripheral blood of asthmatics and its effect on immune cells (Th2, Th17, Tregs) involved in the pathogenesis of asthma.
  
  
  METHODS
  Forty-seven asthmatics from Ruijin Hospital were recruited and assigned to intermittent, mild and moderate-severe groups. Lung function test and Asthma Control Questionnaire were performed to evaluate asthma control and severity. Twenty healthy donors were enrolled as controls. GATA3, IL-4, and HDAC9 mRNA expression levels were measured by SYRB Green Real-time PCR. The cytokine IL-17-mainly produced by Th17 cells and TGF-β-mainly produced by Treg cells, were measured by ELISA.
  
  
  RESULTS
  The GATA3 and IL-4 mRNA expression levels (28.12 ± 7.57 and 743.6 ± 312.8) were up-regulated in asthmatics as compared to the healthy controls [0.56 ± 0.22, 0.7 ± 0.8 (U = 16.00, 37.00, P < 0.01)]. The HDAC9 mRNA expression levels of intermittent, mild and moderate-severe groups were 3.20 ± 0.50, 89.6 ± 18.0, 323.0 ± 65.3, respectively, which were associated with the severity of disease (H = 11.32, P < 0.05). The level of IL-17 in asthmatic group was (83 ± 55) ng/L, which was up-regulated as compared to the healthy control [(34 ± 22) ng/L (U = 153.50, P < 0.01)]. The level of TGF-β was decreased in the asthmatic groups as compared to the healthy control, but the difference did not reach significance. HDAC9 mRNA expression level was positively correlated with GATA3 mRNA expression level (r = 0.482, P < 0.05), and also with IL-4 mRNA expression (r = 0.432, P < 0.05) and IL-17 (r = 0.538, P < 0.05), but negatively correlated with TGF-β (r = -0.417, P < 0.05). In patients with moderate-severe asthma, HDAC9 mRNA expression level was negatively correlated with FEV(1)% (r = -0.657, P < 0.05).
  
  
  CONCLUSIONS
  HDAC9 mRNA expression was up-regulated in peripheral blood of asthmatics, which was not only associate with the Th2 master transcriptional factors GATA3, cytokine IL-4 mRNA, Th17 and Treg cell-related cytokines, but also with FEV(1)% in moderate-severe asthma.
authors.affiliation: Department of Pulmonary Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China.
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Hou
authors.forename: Guo-chao
authors.initials: GC
authors.lastname: Shi
authors.forename: Huan-ying
authors.initials: HY
authors.lastname: Wan
authors.forename: Xiang-yan
authors.initials: XY
authors.lastname: Ai
authors.forename: Yu-heng
authors.initials: YH
authors.lastname: Shi
authors.forename: Ying-meng
authors.initials: YM
authors.lastname: Ni
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood* / Q000276:immunology; D016022:Case-Control Studies; D005260:Female; D050990:GATA3 Transcription Factor / Q000097:blood*; D006655:Histone Deacetylases / Q000097:blood*; D006801:Humans; D020381:Interleukin-17 / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012097:Repressor Proteins / Q000097:blood*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D058504:Th17 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D055815:Young Adult
chemical_list: D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C508598:IL4 protein, human; D020381:Interleukin-17; D012097:Repressor Proteins; D015847:Interleukin-4; C486544:HDAC9 protein, human; D006655:Histone Deacetylases