title: Pseudomonas linyingensis sp. nov.: a novel bacterium isolated from wheat soil subjected to long-term herbicides application.
abstract: A strain of genus Pseudomonas, LYBRD3-7(T) was isolated from long-term sulfonylurea herbicides applied wheat-field soil in Linying located in Henan province of China. This strain is a strictly aerobic and Gram-negative short rod-shaped bacterium with single flagellum. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this isolate as a member of Pseudomonas, and most closely to Pseudomonas tuomuerensis CGMCC 1.1365(T) (97.1 %) and P. alcaligenes IAM12411(T) (97.1 %). Morphological characters and chemotaxonomic data confirmed the affiliation of strain LYBRD3-7(T) to the genus Pseudomonas. The results of phylogenetic analysis, physiological and biochemical studies, and DNA-DNA hybridization allowed the differentiation of genotype and phenotype between strain LYBRD3-7(T) and the phylogenetic closest species with valid names. The name proposed for the new species is Pseudomonas linyingensis sp. nov. The type strain is LYBRD3-7(T) (=CGMCC 1.10701(T ) =LMG 25967(T)).
authors.affiliation: Henan Key Laboratory of Microbial Engineering, Institute of Biology, Henan Academy of Sciences, Zhengzhou, China.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: He
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Wang
authors.forename: Xun
authors.initials: X
authors.lastname: Du
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Jia
authors.forename: Jiang
authors.initials: J
authors.lastname: Bian
authors.forename: Shuang-Jiang
authors.initials: SJ
authors.lastname: Liu
authors.forename: Guo-Can
authors.initials: GC
authors.lastname: Chen
mesh_terms: D002681:China; D006540:Herbicides / Q000032:analysis*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*; D014908:Triticum / Q000254:growth & development*
chemical_list: D006540:Herbicides; D012989:Soil Pollutants