title: Evaluation of sequence-specific priming and real-time polymerase chain reaction assays for detecting HLA-B*51 alleles confirmed by sequence-based typing.
abstract: The human leukocyte antigen (HLA)-B*51 genotype is one of the well-known genetic factors associated with the development of Behcet's disease. We evaluated three sequence-specific priming (SSP) assays and one real-time PCR assay for detecting HLA-B*51 alleles using 93 whole blood samples, which were genotyped by high-resolution sequence-based typing (SBT). All HLA-B*51 alleles determined by SBT were detected by the four evaluated assays, and the results for all HLA-B alleles other than HLA-B*51 were negative on all assays. Thus, all HLA-B51 tests showed 100% sensitivity and 100% specificity for detecting HLA-B*51 alleles. The three SSP assays and the real-time PCR test for HLA-B*51 genotyping are simple, but reliable for detecting HLA-B*51 alleles in clinical laboratories.
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, Seodaemun-gu, Seoul, South Korea.
authors.forename: Y
authors.initials: Y
authors.lastname: Park
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
authors.forename: S I
authors.initials: SI
authors.lastname: Kim
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: H S
authors.initials: HS
authors.lastname: Kim
mesh_terms: D000483:Alleles; D001528:Behcet Syndrome / Q000175:diagnosis* / Q000235:genetics* / Q000276:immunology; D005838:Genotype; D059949:HLA-B51 Antigen / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D059949:HLA-B51 Antigen