title: Fast genomic biomarker responses of retene and pyrene in liver of juvenile rainbow trout, Oncorhynchus mykiss.
abstract: We studied the transcriptive effects of two PAHs, retene (RET) and pyrene (PYR), in three equimolar sublethal concentrations (0.9-10 μg/L) in the liver of juvenile rainbow trout, Oncorhynchus mykiss. After 24 h of in vivo exposure, expressions of selected genes (CYP1A, Hsp30, Hsp70, Grp78, Sep15, GP1) were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). These PAHs changed the studied gene transcriptions differently, but not significantly, except for CYP1A, which was induced only by RET. RET induced CYP1A gene expression even at low, environmentally realistic concentrations in the liver of juvenile rainbow trout.
authors.affiliation: Department of Biological and Environmental Sciences, Section of Environmental Science and Technology, University of Jyväskylä, Survontie 9, 40014 Jyväskylä, Finland. kati.m.rasanen@jyu.fi
authors.forename: Kati
authors.initials: K
authors.lastname: Räsänen
authors.forename: Tiina
authors.initials: T
authors.lastname: Arsiola
authors.forename: Aimo
authors.initials: A
authors.lastname: Oikari
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D004784:Environmental Monitoring; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D017686:Oncorhynchus mykiss; D010616:Phenanthrenes / Q000633:toxicity*; D011721:Pyrenes / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D029941:Fish Proteins; D006360:Heat-Shock Proteins; D010616:Phenanthrenes; D011721:Pyrenes; D014874:Water Pollutants, Chemical; C447880:retene; C030984:pyrene; D019363:Cytochrome P-450 CYP1A1