title: Programed nebulizing-gas pressure mode for quantitative capillary electrophoresis-mass spectrometry analysis of endocrine disruptors in honey.
abstract: The application of programed nebulizing-gas pressure (PNP) has been previously described to be a simple strategy for the separation of anions by capillary electrophoresis-electrospray-mass spectrometry (CE-ESI-MS). The PNP mode provided high resolution and stable analyses and also had the advantage of allowing the use of capillaries wider than the 50-75 μm conventional ones. Here, the application of the PNP approach to the quantitative analysis of pollutants in real samples by CE-ESI-MS is described for the first time; in particular, for the determination several endocrine disruptors (2,4-dichlorophenol, 2,4,5-trichlorophenol, pentachlorophenol, bisphenol-A, 4-tert-butyl-phenol, and 4-tert-butyl benzoic acid) in honey. For sample pretreatment, different liquid-liquid extraction (LLE) procedures were assayed and compared to the QuEChERS(©) methodology prior to electrophoretic analysis. With the application of the PNP approach to CE-ESI-MS, the limits of detection achieved were in the 1-4 ng/g range with a simple liquid-liquid procedure without any further clean-up step; relative standard deviation values in the 2-9% range were found. The analytical characteristics allow the proposed method to be used in the control analysis of these compounds in honey.
authors.affiliation: Departamento de Química Analítica, Nutrición y Bromatología, University of Salamanca, Salamanca, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Domínguez-Álvarez
authors.forename: Encarnación
authors.initials: E
authors.lastname: Rodríguez-Gonzalo
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernández-Méndez
authors.forename: Rita
authors.initials: R
authors.lastname: Carabias-Martínez
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D052244:Endocrine Disruptors / Q000032:analysis* / Q000302:isolation & purification; D018857:Food Packaging; D006722:Honey / Q000032:analysis*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D052244:Endocrine Disruptors; D010636:Phenols