title: Relationship between urinary nephrin and urinary albumin changes in diabetic rats and effects of yiqiyangyinhuayutongluo recipe.
abstract: OBJECTIVE
  To investigate the dynamic changes of urinary nephrin, and the relationship between it and urinary albumin excretion rate (UAER) in a diabetic rat model, as well the effects of yiqiyangyinhuayutongluo recipe.
  
  
  METHODS
  Diabetic model was induced by high fat diet combined with low-dose Streptozotocin (STZ) in rats. Normal group (NG), model group (MG), and yiqiyangyinhuayutongluo recipe treated group (YHTG) were set. Gastrointestinal Yiqiyangyi-nhuayutongluo recipe was administered once daily for 32 w. At the end of the 2nd w (2 w), 8 w, 16 w, and 32 w, fasting blood glucose (FBG), UAER and 24h urinary nephrin (U-nephrin) were detected.
  
  
  RESULTS
  Compared with NG, FBG in MG increased notably (P < 0.05). Compared with MG, FBG of YHTG (P < 0.05) since 16 w. U-nephrin and UAER in MG increased significantly from 2 w, peaked at 16 w, lessened in different degree at 32 w, but were still higher than NG. The correlation analysis showed that there was a significant positive correlation between U-nephrin and UAER at different time, the correlation coefficient as r > 0.9, and P < 0.05. Compared with MG, U-nephrin and UAER in YHTG decreased markedly (P < 0.05) except for U-nephrin at 8 w.
  
  
  CONCLUSIONS
  U-nephrin and UAER in diabetic rat model have a positive linear correlation. Yiqiyangyinhuayutongluo recipe can reduce UAER markedly, and preventing the lose of nephrin in urine maybe one of the mechanisms.
authors.affiliation: Institute of Integration of Traditional Chinese Medicine-Western Medicine, Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Li-li
authors.initials: LL
authors.lastname: Li
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Yue-hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Jiang-hua
authors.initials: JH
authors.lastname: Zhang
authors.forename: Zhi-wei
authors.initials: ZW
authors.lastname: Yin
authors.forename: Lin-lin
authors.initials: LL
authors.lastname: Li
authors.forename: Xue-yun
authors.initials: XY
authors.lastname: Zhang
authors.forename: Feng-li
authors.initials: FL
authors.lastname: Wang
mesh_terms: D000419:Albuminuria / Q000517:prevention & control*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000652:urine; D003928:Diabetic Nephropathies / Q000517:prevention & control*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D008297:Male; D008565:Membrane Proteins / Q000652:urine*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D008565:Membrane Proteins; C113267:nephrin; C548799:yiqi yangyin; D013311:Streptozocin