title: A cis-regulatory module upstream of deltaC regulated by Ntla and Tbx16 drives expression in the tailbud, presomitic mesoderm and somites.
abstract: Somites form by an iterative process from unsegmented, presomitic mesoderm (PSM). Notch pathway components, such as deltaC (dlc) have been shown to play a role in this process, while the T-box transcription factors Ntla and Tbx16 regulate somite formation upstream of this by controlling supply and movement of cells into the PSM during gastrulation and tailbud outgrowth. In this work, we report that Ntla and Tbx16 play a more explicit role in segmentation by directly regulating dlc expression. In addition we describe a cis-regulatory module (CRM) upstream of dlc that drives expression of a reporter in the tailbud, PSM and somites during somitogenesis. This CRM is bound by both Ntla and Tbx16 at a cluster of T-box binding sites, which are required in combination for activation of the CRM.
authors.affiliation: Department of Physiology, Development and Neuroscience, Cambridge University, Downing Street, Cambridge, CB2 3DY, UK. Jahangiri.Leila@mgh.harvard.edu
authors.forename: Leila
authors.initials: L
authors.lastname: Jahangiri
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Nelson
authors.forename: Fiona C
authors.initials: FC
authors.lastname: Wardle
mesh_terms: D000818:Animals; D001483:Base Sequence; D047369:Chromatin Immunoprecipitation; D017931:DNA Primers / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D005326:Fetal Proteins; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D008648:Mesoderm / Q000196:embryology / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D019170:Somites / Q000196:embryology / Q000378:metabolism*; D020825:T-Box Domain Proteins / Q000235:genetics / Q000494:pharmacology*; D013623:Tail / Q000196:embryology / Q000378:metabolism*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000494:pharmacology*; D000098908:Brachyury Protein
chemical_list: D017931:DNA Primers; D005326:Fetal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D020825:T-Box Domain Proteins; D029961:Zebrafish Proteins; D000098908:Brachyury Protein; C064045:delta protein; C112539:tbx16 protein, zebrafish