title: Cyclooxygenase-2-765G>C functional promoter polymorphism and its association with oral squamous cell carcinoma.
abstract: OBJECTIVE
  Cyclooxygenase-2 is a key enzyme in the conversion of arachidonic acid to prostaglandins, and has critical role in the progression of several malignancies, including oral squamous cell carcinoma.
  
  
  METHODS
  We designed a case-control study to evaluate the susceptibility of the functional -765G>C genetic variation in oral squamous cell carcinoma patients. Polymerase chain reaction-based restriction fragment length polymorphism analysis was used to determine the polymorphism in oral squamous cell carcinoma (n = 150) patients and healthy controls (n = 150).
  
  
  RESULTS
  The genotype frequencies of cyclooxygenase-2 765G>G, 765G>C, and 765C>C were 73.3%, 18.66%, and 8.0% in the cancer patients, and 94.66%, 4% and 1.3% in the controls, respectively. The cyclooxygenase-2 GC and CC genotypes were significantly associated (P = 0.0003 and P = 0.01, respectively) with oral squamous cell carcinoma patients, when compared to the controls. The 765G>C genotypes were statistically significant, with habitual tobacco chewing and alcohol consumption + smoking (P = 0.05).
  
  
  CONCLUSIONS
  This study highlights the genetic variant that might play a role in mediating susceptibility to oral squamous cell carcinoma in this population.
authors.affiliation: Mehdi Nawaj Jung Bahadur Cancer Hospital, Hyderabad, Andhra Pradesh, India. laxmiaddala@gmail.com
authors.forename: Addala
authors.initials: A
authors.lastname: Lakshmi
authors.forename: Sadhnani
authors.initials: S
authors.lastname: Muralidhar
authors.forename: Chennaboina
authors.initials: C
authors.lastname: Kalyan Kumar
authors.forename: Addala
authors.initials: A
authors.lastname: Pavan Kumar
authors.forename: Pentapati
authors.initials: P
authors.lastname: Kalyana Chakravarthy
authors.forename: Vasala
authors.initials: V
authors.lastname: Anjaneyulu
authors.forename: Jamil
authors.initials: J
authors.lastname: Kaiser
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000428:Alcohol Drinking / Q000009:adverse effects / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects / Q000378:metabolism
chemical_list: D051546:Cyclooxygenase 2