title: [Differential gene expression profiles of gastric cancer].
abstract: OBJECTIVE
  To explore the different gene expressions of normal versus tumor tissues of gastric cancer at molecular levels.
  
  
  METHODS
  Gene chip technology was used to determine the differentially expressed genes between gastric cancer (n = 12) and normal tissues (n = 12) from December 2009 to June 2010 of Xinhua Hospital of Shanghai Jiaotong University School of Medicine. And reverse transcriptase (RT)-PCR was performed to validate the results of gene chip analysis.
  
  
  RESULTS
  Sixty-nine up-regulated genes and 80 down-regulated genes were identified by significance analysis of microarrays (SAM). And these genes were correlated with cell adhesion, angiogenesis, cell proliferation and apoptosis, et al. They were also closely correlated with the signaling pathways of Wnt (1/151, 0.66%) and vascular endothelial growth factor (VEGF) (2/76, 2.63%). The differential expressions of ATP4A, CLDN10, OLFM4, SAA1 and PROK2 were confirmed by RT-PCR (0.94 ± 0.19 vs 4.33 ± 0.39, 1.00 ± 0.14 vs 3.04 ± 0.26, 5.37 ± 0.30 vs 1.02 ± 0.14, 4.37 ± 0.30 vs 0.95 ± 0.29, 2.62 ± 0.54 vs 1.35 ± 0.35, all P < 0.05).
  
  
  CONCLUSIONS
  The classifier genes identified in this study may be closely correlated with the carcinogenesis of gastric cancer.
authors.affiliation: Department of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Mao-Lan
authors.initials: ML
authors.lastname: Li
authors.forename: Song-Gang
authors.initials: SG
authors.lastname: Li
authors.forename: Wen-Guang
authors.initials: WG
authors.lastname: Wu
authors.forename: Long-Hua
authors.initials: LH
authors.lastname: Rao
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Qi-Chen
authors.initials: QC
authors.lastname: Ding
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Yang
authors.forename: Xiang-Song
authors.initials: XS
authors.lastname: Wu
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Lu
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Ying-Bin
authors.initials: YB
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology*; D005773:Gastroscopy; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*