title: Alteration of the inflammatory molecule network after irradiation of soft tissue.
abstract: Inflammatory molecules (IMs) play an important role in ionizing radiation (IR)-induced soft tissue damage. The alteration of IMs as a function of time was studied with a protein array containing 62 IMs in mouse cutaneous soft tissues exposed to 30 Gy. The results showed that: (1) 2 days after irradiation, the levels of TGF-β1, MIP-1γ, IL-1α, and sTNF RI increased, while IGFBP-3, CXCL16, and IL-1β decreased in IR skin as compared to control skin; (2) 21 days after IR, TGF-β1, and MIP-1 γ, IL-1α remained high, while CXCL16 and IL-1β remained low; (3) 3 months after IR, the cytokine pattern exhibited reversals. The levels of MIP-1γ decreased, while VCAM-1, IGFBP-3, and TGF-β1 production increased. The data indicated that: (a) IMs change as a function of time after soft tissue irradiation; (b) changing IM levels may reflect the altered balance of the cytokine network, leading to imbalance or homeostasis; and (c) an antibody-based protein array can be used to assess multiple IMs simultaneously, making it useful for bulk screening for changes in tissue cytokine levels.
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Shanmin
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Hengshan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Kunzhong
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Yeping
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Yongbing
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Liangjie
authors.initials: L
authors.lastname: Yin
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA.
authors.forename: Lurong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 103633, Gainesville, FL, 32610, USA. pokunieff@ufl.edu.
authors.forename: Paul
authors.initials: P
authors.lastname: Okunieff
mesh_terms: D000818:Animals; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006614:Hindlimb / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects*; D018836:Inflammation Mediators / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D040081:Protein Array Analysis; D012867:Skin / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects*; D017695:Soft Tissue Injuries / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors
chemical_list: D018925:Chemokines; D016207:Cytokines; D018836:Inflammation Mediators; D036341:Intercellular Signaling Peptides and Proteins