title: Total reflection X-ray fluorescence spectrometry as a tool for the quantification of gold and platinum metallodrugs: determination of recovery rates and precision in the ppb concentration range.
abstract: Total reflection X-ray spectrometry is an instrumental technique based on X-ray fluorescence, which offers detection limits low enough to quantify trace element concentrations with negligible interference from matrix components. The technique is well established in material sciences and now reaches out to extended applications in life sciences and pharmaceutical quality control. In our study we focused on possible applications for the quantification of gold and platinum containing active ingredients in trace concentrations (ppb range) in a matrix of biological origin (cell suspensions). General aspects of sample preparation as well as selected important method performance parameters (precision, recovery rates) were investigated. Overall, TXRF represents an useful option to quantify metals in ppb concentrations with acceptable precision and recovery.
authors.affiliation: Institute of Medicinal and Pharmaceutical Chemistry, Technische Universität Braunschweig, Beethovenstrasse 55, 38106 Braunschweig, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Meyer
authors.forename: Sandra
authors.initials: S
authors.lastname: Grotefend
authors.forename: Armin
authors.initials: A
authors.lastname: Gross
authors.forename: Hermann
authors.initials: H
authors.lastname: Wätzig
authors.forename: Ingo
authors.initials: I
authors.lastname: Ott
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis* / Q000378:metabolism; D018501:Antirheumatic Agents / Q000032:analysis* / Q000378:metabolism; D001310:Auranofin / Q000032:analysis* / Q000378:metabolism; D002138:Calibration; D002457:Cell Extracts / Q000737:chemistry; D002945:Cisplatin / Q000032:analysis* / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D057230:Limit of Detection; D010865:Pilot Projects; D012015:Reference Standards; D015203:Reproducibility of Results; D013052:Spectrometry, X-Ray Emission / Q000592:standards
chemical_list: D000970:Antineoplastic Agents; D018501:Antirheumatic Agents; D002457:Cell Extracts; D001310:Auranofin; D002945:Cisplatin