title: Effect of testosterone incorporation on cell proliferation and differentiation for polymer-bioceramic composites.
abstract: In the current study, we characterized the polycaprolactone (PCL), poly(lactic acid-co-glycolic acid) (PLGA), and biphasic calcium phosphate (BCP) composites coated with testosterone propionate (T) using Fourier transform infrared spectroscopy (FTIR) and powder X-ray diffraction (XRD). Osteoblastic cells were seeded with PCL/BCP, PCL/BCP/T, PLGA/PCL/BCP and PLGA/PCL/BCP/T scaffolds, and cell viability, proliferation, differentiation and adhesion were analyzed. The results of physic-chemical experiments showed no displacements or suppression of bands in the FTIR spectra of scaffolds. The XRD patterns of the scaffolds showed an amorphous profile. The osteoblastic cells viability and proliferation increased in the presence of composites with testosterone over 72 h, and were significantly greater when PLGA/PCL/BCP/T scaffold was tested against PCL/BCP/T. Furthermore alkaline phosphatase production was significantly greater in the same group. In conclusion, the PLGA/PCL/BCP scaffold with testosterone could be a promising option for bone tissue applications due to its biocompatibility and its stimulatory effect on cell proliferation.
authors.affiliation: Departamento de Odontologia Restauradora, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Kelen Jorge Rodrigues
authors.initials: KJ
authors.lastname: da Costa
authors.forename: Joel J
authors.initials: JJ
authors.lastname: Passos
authors.forename: Alinne D M
authors.initials: AD
authors.lastname: Gomes
authors.forename: Rubén D
authors.initials: RD
authors.lastname: Sinisterra
authors.forename: Célia R M
authors.initials: CR
authors.lastname: Lanza
authors.forename: Maria Esperanza
authors.initials: ME
authors.lastname: Cortés
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002516:Ceramics / Q000737:chemistry*; D008297:Male; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D033362:Powder Diffraction; D051381:Rats; D017208:Rats, Wistar; D017550:Spectroscopy, Fourier Transform Infrared; D013739:Testosterone / Q000494:pharmacology*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D013739:Testosterone; D000469:Alkaline Phosphatase