title: Biofunctionalization of titanium surface with multilayer films modified by heparin-VEGF-fibronectin complex to improve endothelial cell proliferation and blood compatibility.
abstract: In-stent restenosis and subsequent thrombosis remain a significant complication following the implantation of coronary stents. Different approaches have been used in developing novel coronary stents to protect against thrombosis and minimize restenosis. In the present study, we designed a biomacromolecular layer-by-layer coating with heparin, vascular endothelial growth factor (VEGF), and fibronectin onto nickel-free titanium surface to improve blood compatibility and endothelial cell proliferation. The multilayer assembling process was monitored by water contact angle and surface plasmon resonance, respectively. With increasing the number of layers, the deposition of polyelectrolyte as self-assembled ultrathin multilayer films showed linear growth of absorbance. In vitro blood compatibility results revealed that the fabricated layers prolonged activated partial thrombin time and prothrombin time, reduced platelets activation and aggregation, and reduced blood hemolysis rate. Cell adhesion and growth results showed that the assembled multilayer films significantly promoted cell attachment and growth, and the endothelialization property of the multilayer films was preferable compared with the untreated titanium disk. In conclusion, these results suggest that titanium surface modification using biofunctional multilayer films composed of heparin, VEGF, and fibronectin may serve as a potential approach to inhibit thrombosis and promote re-endothelialization of cardiovascular stents.
authors.affiliation: Key Laboratory of Biological Science and Technology, Ministry of Education, Chongqing University, Chongqing 400044, People's Republic of China.
authors.forename: H G
authors.initials: HG
authors.lastname: Wang
authors.forename: T Y
authors.initials: TY
authors.lastname: Yin
authors.forename: S P
authors.initials: SP
authors.lastname: Ge
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: Q L
authors.initials: QL
authors.lastname: Dong
authors.forename: D X
authors.initials: DX
authors.lastname: Lei
authors.forename: D M
authors.initials: DM
authors.lastname: Sun
authors.forename: G X
authors.initials: GX
authors.lastname: Wang
mesh_terms: D000042:Absorption; D001672:Biocompatible Materials / Q000494:pharmacology*; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D005353:Fibronectins / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008422:Materials Testing; D010314:Partial Thromboplastin Time; D010973:Platelet Adhesiveness / Q000187:drug effects; D010976:Platelet Count; D011094:Polyethyleneimine / Q000494:pharmacology; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D042461:Vascular Endothelial Growth Factor A; D011094:Polyethyleneimine; D006493:Heparin; D014025:Titanium