title: Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: effects on the cell apoptosis through mitochondrial-related and ER stress pathway.
abstract: An oral squamous cell carcinoma Ca9-22 cell line was treated with 11-dehydrosinulariolide, an active compound isolated from the soft coral Sinularia leptoclados, in order to evaluate the effect of this compound on cell growth and protein expression. Cell proliferation was strongly inhibited by 11-dehydrosinulariolide treatment. The 2-DE master maps of control and treated Ca9-22 cells were generated by analysis with the PDQuest software. The comparison between such maps showed up- and down-regulation of 23 proteins, of which 14 were upregulated and 9 were downregulated. The proteomic studies described here have identified some proteins, which are involved in the mitochondrial dysfunction and ER-stress pathway and imply that 11-dehydrosinulariolide induces cell apoptosis through either mitochondrial dysfunction-related or ER stress pathway. Based on this observation, several proteins related to apoptosis pathway were explored for the potential roles involved in this drug-induced cytotoxicity. Furthermore, Salubrinal, an ER stress inhibitor, is able to protect the cell from 11-dehydrosinulariolide-induced apoptosis in a physiological dosage. The significance of these studies illustrates the potential development of anticancer drugs from the natural derivatives of soft coral.
authors.affiliation: Department of Nursing, Meiho University, Pingtung, Taiwan.
authors.forename: Chih-I
authors.initials: CI
authors.lastname: Liu
authors.forename: Robert Yung-Liang
authors.initials: RY
authors.lastname: Wang
authors.forename: Jen-Jie
authors.initials: JJ
authors.lastname: Lin
authors.forename: Jui-Hsin
authors.initials: JH
authors.lastname: Su
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu
authors.forename: Jiing-Chuan
authors.initials: JC
authors.lastname: Chen
authors.forename: Jeff Yi-Fu
authors.initials: JY
authors.lastname: Chen
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Wu
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002934:Cinnamates / Q000627:therapeutic use; D004224:Diterpenes / Q000627:therapeutic use*; D015180:Electrophoresis, Gel, Two-Dimensional; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D009062:Mouth Neoplasms / Q000188:drug therapy*; D040901:Proteomics; D013890:Thiourea / Q000031:analogs & derivatives / Q000627:therapeutic use
chemical_list: C569844:11-dehydrosinulariolide; D000970:Antineoplastic Agents; D002934:Cinnamates; D004224:Diterpenes; C496827:salubrinal; D013890:Thiourea