title: Automated lineage and expression profiling in live Caenorhabditis elegans embryos.
abstract: Describing gene expression during animal development requires a way to quantitatively measure expression levels with cellular resolution and to describe how expression changes with time. Fluorescent protein reporters make it possible to measure expression dynamics in live cells by time-lapse microscopy, but it can be challenging to identify expressing cells in complex tissues and to compare expression across organisms. This protocol describes how to use automated lineage analysis to identify cells in Caenorhabditis elegans embryos expressing fluorescent reporters and how to quantify that expression with cellular resolution. Because C. elegans develops through an invariant pattern of cell divisions, every cell's identity and future fate can be predicted from its pattern of previous cell divisions. Automated analysis of images collected from embryos expressing a fluorescent histone transgene in all cells allows lineage tracing and cell identification. This provides a scaffold with which to describe expression of a second color reporter such as a fusion of a second fluorescent protein to a gene of interest or its regulatory sequences. These methods can also be used for analysis of reporter expression, cell division timing, and cell position in genetically perturbed embryos. The protocol describes how to prepare C. elegans strains containing nuclear-expressed fluorescent reporters, collect images of appropriate quality from embryos, perform automated lineage analysis, manually edit and curate the lineage, and, finally, extract and display reporter signals.
authors.forename: John Isaac
authors.initials: JI
authors.lastname: Murray
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Bao
mesh_terms: D000818:Animals; D057205:Automation, Laboratory / Q000379:methods*; D017173:Caenorhabditis elegans / Q000145:classification / Q000196:embryology* / Q000235:genetics* / Q000254:growth & development; D004625:Embryo, Nonmammalian; D005453:Fluorescence; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D005838:Genotype; D007091:Image Processing, Computer-Assisted / Q000379:methods