title: Immune system stimulation reduces the efficiency of tryptophan utilization for body protein deposition in growing pigs.
abstract: The effect of immune system stimulation (ISS) on N retention and Trp utilization in pigs fed Trp-limiting diets was evaluated using 36 growing pigs (20.0 ± 1.1 kg BW; 3 blocks of 12 barrows). Pigs were randomly assigned to 1 of 5 diets (Diet 1, 2, 4, and 5, n = 7; Diet 3, n = 8) and fed restrictively at 800 g/d. Diets 1 to 4 were generated by blending Diet 1 with a protein-free supplement and were calculated to contain varying amounts of standardized ileal digestible (SID) Trp (1.31, 1.05, 0.80, and 0.55 g/kg). To confirm that Trp was the first-limiting AA in Diets 1 to 4, an additional diet was used (Diet 5), which was equivalent to Diet 4 and contained 0.34 g/kg of added Trp. After a 5-d adaptation period, pigs were injected every 2 d with increasing amounts of E. coli lipopolysaccharide to induce ISS (initial dose 20 μg/kg BW, increasing 15% each subsequent injection). Whole body N balance was measured in 3 periods: before immune stimulation (pre-ISS) and during ISS in 2 subsequent periods (ISS-1, 3 d; ISS-2, 4 d). Regression analysis was used to estimate the marginal efficiency of Trp utilization for whole body protein deposition (PD; N retention × 6.25). Plasma concentrations of acute-phase proteins and white blood cell counts increased (P < 0.001) and plasma albumin decreased (P < 0.001) during ISS. Nitrogen retention increased (P < 0.001) as Trp intake increased. Nitrogen retention was numerically greater but not statistically different between Diet 5 (added Trp diet) and Diet 4. Whole body N retention was less (P < 0.05) during ISS due primarily to an increase (P < 0.05) in urinary N excretion. There was a linear response (P < 0.05) in N retention, urinary N, and total excreted N to increasing Trp intake. Protein deposition increased by 88.2 ± 5.2, 82.5 ± 5.1, and 92.5 ± 3.4 g/d for each additional g/d of SID Trp intake during pre-ISS, ISS-1, and ISS-2, respectively, but the intercept was not different (-32.3 g/d). The slope of the response of PD to increasing Trp intake (based on the common intercept) was less during ISS-1 compared with pre-ISS (P = 0.01) or ISS-2 (P = 0.002) but not different between pre-ISS and ISS-2. Immune system stimulation reduced N retention in pigs fed limiting dietary Trp. The efficiency of Trp utilization for protein deposition was also reduced during ISS, indicating that the Trp requirement for PD is increased ∼7% during an inflammatory state.
authors.affiliation: Department of Animal and Poultry Science, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: K
authors.initials: K
authors.lastname: de Ridder
authors.forename: C L
authors.initials: CL
authors.lastname: Levesque
authors.forename: J K
authors.initials: JK
authors.lastname: Htoo
authors.forename: C F M
authors.initials: CF
authors.lastname: de Lange
mesh_terms: D056704:Adaptive Immunity; D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004032:Diet; D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000008:administration & dosage; D009584:Nitrogen / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D012044:Regression Analysis; D013552:Swine / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D014364:Tryptophan / Q000008:administration & dosage / Q000276:immunology* / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D011506:Proteins; D014364:Tryptophan; D009584:Nitrogen