title: [Phaseoloideside E induces human hepatoma HepG2 cells apoptosis].
abstract: OBJECTIVE
  To study in vitro anti-tumor activity of phaseoloideside E (PE) with human hepatoma HepG2 cells as the objective.
  
  
  METHODS
  MTT assay was adopted to detect the cytotoxic effect of PE of different concentrations on HepG2 cells after being processed for 48 h. Changes in morphology of PE-processed cells were observed under an optical microscope and fluorescence microscope. DNA agrose gel electrophoresis was used to detect the DNA ladder, an important characteristic of cell apoptosis. The expression levels of Bax and Bcl-2 were determined by western blot assay.
  
  
  RESULTS
  PE dramatically repressed the viability of HepG2 cells. Typical morphological changes of apoptosis had been detected by both direct microscopic observation and Hoechst 33,258 staining. Typical DNA Ladder was also observed by agarose gel electrophoresis in the administration group, but it did not exist in the control group. Western blot showed that the expression of Bax was up-regulated and Bcl-2 was down-regulated.
  
  
  CONCLUSIONS
  Above data demonstrates that PE can induce apoptosis in human hepatoma HepG2 cells, and indicate that PE-induced expression level changes of Bax and Bcl2 may be related to the apoptosis-induction effect.
authors.affiliation: College of Pharmacy, South Central University for Nationalities, Wuhan 430074, China. moshsha533@gmail.com
authors.forename: Shasha
authors.initials: S
authors.lastname: Mo
authors.forename: Guangwen
authors.initials: G
authors.lastname: Shu
authors.forename: Hui
authors.initials: H
authors.lastname: Xiong
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Zhanzhan
authors.initials: Z
authors.lastname: Yang
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Mei
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D012503:Saponins / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000032:analysis
chemical_list: D000970:Antineoplastic Agents; C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D012503:Saponins; D014315:Triterpenes; D051028:bcl-2-Associated X Protein; C574496:phaseoloideside E