title: Green tea extract: possible mechanism and antibacterial activity on skin pathogens.
abstract: Camellia sinensis (tea) is known for its therapeutic properties (anti-inflammatory, anti-microbial, anti-tumour, anti-oxidative and anti-ageing). Although, anti-microbial properties of green tea have been studied, its role against bacterial strains related to skin infections and mechanism of action is not well understood. We focussed on exploring anti-microbial activity and the basic mechanism of aqueous green tea leaf extract on selected bacterial strains. Staphylococcus epidermidis, Micrococcus luteus, Brevibacterium linens, Pseudomonas fluorescens and Bacillus subtilis were found to be sensitive to green tea extract via disc diffusion assay (zone of inhibition ≥7 mm). Minimal inhibitory concentration (MIC) was determined via nitro blue tetrazolium (NBT) assay (0.156-0.313 mg/ml). Moreover, the aqueous extract was found to be not toxic to the Vero cell-line up to a concentration of 500 μg/ml. The effect of aqueous extract on adhesion of different bacteria to Vero cells indicated that it inhibits the adhesion at its MIC value.
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, 201307 UP, India. anjalish1988@gmail.com
authors.forename: Anjali
authors.initials: A
authors.lastname: Sharma
authors.forename: Sonal
authors.initials: S
authors.lastname: Gupta
authors.forename: Indira P
authors.initials: IP
authors.lastname: Sarethy
authors.forename: Shweta
authors.initials: S
authors.lastname: Dang
authors.forename: Reema
authors.initials: R
authors.lastname: Gabrani
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D001422:Bacterial Adhesion / Q000187:drug effects; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000032:analysis / Q000494:pharmacology; D002522:Chlorocebus aethiops; D006801:Humans; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D017192:Skin Diseases, Bacterial / Q000188:drug therapy / Q000382:microbiology*; D013662:Tea / Q000737:chemistry*; D014709:Vero Cells
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D013662:Tea; D002392:Catechin