title: High-speed tracking of murine cardiac stem cells by harmonic nanodoublers.
abstract: Potassium niobate nonlinear nanoparticles are used for the first time to monitor the evolution of embryonic stem cells (ESC) by second harmonic microscopy. These particles feature the complete absence of photo-bleaching and unlimited excitation wavelength flexibility. The potential of this approach is made evident for tissue-regeneration studies and applications, by capturing a high-speed movie of ESC-derived cardiomyocytes autonomously beating within a cluster. Time-resolved data are analyzed to retrieve 3D information of the contraction pattern at the cellular level.
authors.affiliation: GAP-Biophotonics, Université de Genève, Switzerland.
authors.forename: Thibaud
authors.initials: T
authors.lastname: Magouroux
authors.forename: Jerome
authors.initials: J
authors.lastname: Extermann
authors.forename: Pernilla
authors.initials: P
authors.lastname: Hoffmann
authors.forename: Yannick
authors.initials: Y
authors.lastname: Mugnier
authors.forename: Ronan
authors.initials: R
authors.lastname: Le Dantec
authors.forename: Marisa E
authors.initials: ME
authors.lastname: Jaconi
authors.forename: Christelle
authors.initials: C
authors.lastname: Kasparian
authors.forename: Daniel
authors.initials: D
authors.lastname: Ciepielewski
authors.forename: Luigi
authors.initials: L
authors.lastname: Bonacina
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Wolf
mesh_terms: D000818:Animals; D053595:Embryonic Stem Cells / Q000166:cytology*; D051379:Mice; D009206:Myocardium / Q000166:cytology*; D036103:Nanotechnology