title: Should I stay or should I go: protection and maintenance of DNA methylation at imprinted genes.
abstract: Recent findings shed light on the coordination of two fundamental, yet mechanistically opposing, processes in the early mammalian embryo. During the oocyte-to-embryo transition and early preimplantation development nuclear reprogramming occurs. This resetting of the epigenome in maternal and paternal pronuclei to a ground state is the essential step ensuring totipotency in the zygote, the first embryonic stage. Radical, global DNA demethylation, which occurs actively in the paternal and passively in the maternal genome, is a prominent feature of nuclear reprogramming; yet, this process poses a danger to a subset of methylated sequences that must be preserved for their germline to soma inheritance. Genomic imprinting and its importance were demonstrated three decades ago by a series of experiments generating non-viable mammalian uniparental embryos. Indeed, imprinted loci, gene clusters with parent-of-origin specific gene expression patterns, must retain their differential methylation status acquired during gametogenesis throughout embryogenesis and in adult tissues. It is just recently that the molecular players that protect/maintain imprinting marks during reprogramming in preimplantation embryos have been identified, in particular, an epigenetic modifier complex formed by ZFP57 and TRIM28/KAP1. The interaction of these and other molecules with the newly formed embryonic chromatin and imprinted genes is discussed and highlighted herein.
authors.affiliation: Mammalian Development Group, Institute of Medical Biology, Singapore. daniel.m@imb.a-star.edu.sg
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Messerschmidt
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming / Q000235:genetics; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans
chemical_list: D002843:Chromatin