title: Thin-layer chromatography-nuclear magnetic resonance spectroscopy - a versatile tool for pharmaceutical and natural products analysisa.
abstract: Thin-layer chromatography (TLC) is a mature and very established technique, frequently used in many fields of applications ranging from natural product analysis to chemical or pharmaceutical applications. The introduction of a commercially available TLC-MS interface was a major  step complementing the ease of use of TLC with structural elucidation power of mass spectrometry (MS). The TLC-MS interface simplifies the workflow dramatically to gain structural information directly from TLC separations. This article describes the potential of TLC-nuclear magnetic resonance  spectroscopy (NMR) utilizing the TLC-MS interface to straightforwardly characterize zones of interest by NMR spectroscopy with a focus on quantification of active pharmaceutical ingredients (API) in formulations and identification of active principles in plant extracts.
authors.affiliation: University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Institute for Chemistry and Bioanalytics, Gründenstrasse 40, CH-4132 Muttenz, Switzerland.
authors.forename: Angelo
authors.initials: A
authors.lastname: Gössi
authors.forename: Uta
authors.initials: U
authors.lastname: Scherer
authors.forename: Götz
authors.initials: G
authors.lastname: Schlotterbeck
mesh_terms: D001688:Biological Products / Q000032:analysis*; D002626:Chemistry, Pharmaceutical; D002855:Chromatography, Thin Layer / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis
chemical_list: D001688:Biological Products; D004364:Pharmaceutical Preparations; D010936:Plant Extracts