title: LuxS-dependent AI-2 regulates versatile functions in Enterococcus faecalis V583.
abstract: Bacteria utilize a quorum sensing (QS) system to coordinate gene expression by monitoring the concentration of molecules known as autoinducers (AI). In the present study, we confirmed the presence of a LuxS/AI-2 dependent QS system in vancomycin-resistant Enterococcus faecalis V583. Then, the cellular targets controlled by AI-2 were identified by comparative proteomics analysis in order to elucidate the possible role of AI-2 in E. faecalis. Results demonstrated 15 proteins that are differentially expressed upon the addition of AI-2, including proteins involved in metabolism, translation, energy production and/or conversion, and cell wall biogenesis. Glyceraldehyde-3-phosphate dehydrogenase and malate dehydrogenase associated with carbohydrate metabolism and energy production were up-regulated upon inducing by AI-2. In addition, externally added AI-2 could down-regulate acetyl-coenzyme A carboxylase and ornithine carbamoyltransferase, two key enzyme involved in metabolism. All these data suggest that AI-2 signaling may play a role in the regulation of a number of important metabolic properties of E. faecali. We further investigated the role of AI-2 in biofilm formation by E. faecalis, showing the addition of AI-2 to E. faecalis V583 cultures resulted in increased biofilm formation. Our results provide important clues to the role of a LuxS/AI-2 dependent QS system in vancomycin-resistant E. faecalis.
authors.affiliation: Institute of Disease Control and Prevention, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, 100071 Beijing, China.
authors.forename: Changlin
authors.initials: C
authors.lastname: Shao
authors.forename: Wei
authors.initials: W
authors.lastname: Shang
authors.forename: Zhan
authors.initials: Z
authors.lastname: Yang
authors.forename: Zhongke
authors.initials: Z
authors.lastname: Sun
authors.forename: Yunmei
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.forename: Xuesong
authors.initials: X
authors.lastname: Wang
authors.forename: Dayang
authors.initials: D
authors.lastname: Zou
authors.forename: Simiao
authors.initials: S
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Lei
authors.forename: Qian
authors.initials: Q
authors.lastname: Cui
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Yin
authors.forename: Xuelian
authors.initials: X
authors.lastname: Li
authors.forename: Xiao
authors.initials: X
authors.lastname: Wei
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: He
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiang
authors.forename: Shuangkui
authors.initials: S
authors.lastname: Du
authors.forename: Xiangru
authors.initials: X
authors.lastname: Liao
authors.forename: Liuyu
authors.initials: L
authors.lastname: Huang
authors.forename: Yufei
authors.initials: Y
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D013293:Enterococcus faecalis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D020869:Gene Expression Profiling; D006714:Homoserine / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D007783:Lactones / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D053038:Quorum Sensing / Q000235:genetics / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D020543:Proteome; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria