title: Mechano growth factor (MGF) promotes proliferation and inhibits differentiation of porcine satellite cells (PSCs) by down-regulation of key myogenic transcriptional factors.
abstract: Porcine satellite cells represent an ideal model system for studying the cellular and molecular basis regulating myogenic stem cell proliferation and differentiation and for exploring the experimental conditions for myoblast transplantation. Here, we investigated the effects of mechano growth factor (MGF), a spliced variant of the IGF-1 gene, on porcine satellite cells. We show that MGF potently stimulated proliferation while inhibited differentiation of porcine satellite cells. MGF-treatment acutely down-regulates the expression of myogenic determination factor (MyoD) and the cyclin-dependent kinase inhibitor p21. MGF-treatment also markedly reduced the overall expression of cyclin B1 and key factors of the myogenic regulatory and myocyte enhancer families, including Myogenein and MEF2A. Taken together, the gene expression data from MGF-treated porcine satellite cells are in favor of a molecular model in which MGF inhibits porcine satellite cell differentiation by down-regulating either the activity or expression of MyoD, which, in turn, suppresses the expression of key genes required for cell cycle progression and differentiation, such as p21, Myogenin, and MEF2. Overall, our findings are in support of the previous suggestion that MGF may be used in vivo and in vitro to promote proliferation of myogenic stem cells to prevent and treat age-related muscle degenerative diseases.
authors.affiliation: College of Animal Science/Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Qin
authors.forename: Xiao-Kui
authors.initials: XK
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.forename: De-Lin
authors.initials: DL
authors.lastname: Mo
authors.forename: Xiong
authors.initials: X
authors.lastname: Tong
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Pan
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Li
authors.forename: Yao-Sheng
authors.initials: YS
authors.lastname: Chen
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Qiao-Ming
authors.initials: QM
authors.lastname: Long
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D024510:Muscle Development / Q000187:drug effects / Q000235:genetics*; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D034421:Sus scrofa; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D017570:MyoD Protein; D014157:Transcription Factors; C523997:mechano-growth factor E, human; D007334:Insulin-Like Growth Factor I