title: The flavonoids hesperidin and rutin promote neural crest cell survival.
abstract: The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endowed with both neural and mesenchymal potentials. The derivatives of the NC at trunk level include neurons and glial cells of the peripheral nervous system in addition to melanocytes, smooth muscle cells and some endocrine cells. Environmental factors control the fate decisions of NC cells. Despite the well-known influence of flavonoids on the central nervous system, the issue of whether they also influence NC cells has not been yet addressed. Flavonoids are polyphenolic compounds that are integral components of the human diet. The biological activities of these compounds cover a very broad spectrum, from anticancer and antibacterial activities to inhibition of bone reabsorption and modulation of inflammatory response. In the present work, we have investigated the actions of the flavonoids hesperidin, rutin and quercetin on NC cells of quail, in vitro. We show for the first time, that hesperidin and rutin increase the viability of trunk NC cells in culture, without affecting cell differentiation and proliferation. The molecular mechanism of this action is dependent on ERK2 and PI3K pathways. Quercetin had no effect on NC progenitors. Taken together, these results suggest that flavonoids hesperidin and rutin increase NC cell survival, which may be useful against the toxicity of some chemicals during embryonic development.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Jader
authors.initials: J
authors.lastname: Nones
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Costa
authors.forename: Rodrigo Bainy
authors.initials: RB
authors.lastname: Leal
authors.forename: Flávia Carvalho Alcantara
authors.initials: FC
authors.lastname: Gomes
authors.forename: Andréa Gonçalves
authors.initials: AG
authors.lastname: Trentin
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006569:Hesperidin / Q000494:pharmacology*; D007150:Immunohistochemistry; D020935:MAP Kinase Signaling System / Q000187:drug effects; D009432:Neural Crest / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011784:Quail; D011794:Quercetin / Q000494:pharmacology; D012431:Rutin / Q000494:pharmacology*
chemical_list: D012431:Rutin; D011794:Quercetin; D006569:Hesperidin; D019869:Phosphatidylinositol 3-Kinases; D048049:Extracellular Signal-Regulated MAP Kinases