title: Adsorption of fibronectin, fibrinogen, and albumin on TiO2: time-resolved kinetics, structural changes, and competition study.
abstract: An understanding of protein adsorption process is crucial for designing biomaterial surfaces. In this work, with the use of a quartz-crystal microbalance with dissipation monitoring, we researched the following: (a) the kinetics of adsorption on TiO(2) surfaces of three extensively described proteins that are relevant for metallic implant integration [i.e., albumin (BSA), fibrinogen (Fbg), and fibronectin (Fn)]; and (b) the competition of those proteins for adsorbing on TiO(2) in a two-step experiment consisted of sequentially exposing the surfaces to different monoprotein solutions. Each protein showed a different process of adsorption and properties of the adlayer-calculated using the Voigt model. The competition experiments showed that BSA displaced larger proteins such as Fn and Fbg when BSA was introduced as the second protein in the system, whereas the larger proteins laid on top of BSA forming an adsorbed protein bi-layer when those were introduced secondly in the system.
authors.affiliation: Department of Material Science and Metallurgy, Technical University of Catalonia, Barcelona, Spain. marta.pegueroles@upc.edu
authors.forename: Marta
authors.initials: M
authors.lastname: Pegueroles
authors.forename: Chiara
authors.initials: C
authors.lastname: Tonda-Turo
authors.forename: Josep A
authors.initials: JA
authors.lastname: Planell
authors.forename: Francisco-Javier
authors.initials: FJ
authors.lastname: Gil
authors.forename: Conrado
authors.initials: C
authors.lastname: Aparicio
mesh_terms: D000327:Adsorption; D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D002417:Cattle; D005340:Fibrinogen / Q000737:chemistry* / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D058525:Quartz Crystal Microbalance Techniques; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D014025:Titanium / Q000737:chemistry*
chemical_list: D001896:Boron Compounds; D005353:Fibronectins; C079804:titanium boride; D012710:Serum Albumin, Bovine; D005340:Fibrinogen; D014025:Titanium