title: Synthesis and characterization of RNA containing a rigid and nonperturbing cytidine-derived spin label.
abstract: The nitroxide-containing nucleoside Çm is reported as the first rigid spin label for paramagnetic modification of RNA by solid-phase synthesis. The spin label is well accommodated in several RNA secondary structures as judged by its minor effect on the thermodynamic stability of hairpin and duplex RNA. Electron paramagnetic resonance (EPR) spectroscopic characterization of mono-, bi-, and trimolecular RNA structures shows that Çm will be applicable for advanced EPR studies to elucidate structural and dynamic aspects of folded RNA.
authors.affiliation: Max Planck Research Group Nucleic Acid Chemistry, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. claudia.hoebartner@mpibpc.mpg.de
authors.forename: Claudia
authors.initials: C
authors.lastname: Höbartner
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Sicoli
authors.forename: Falk
authors.initials: F
authors.lastname: Wachowius
authors.forename: Dnyaneshwar B
authors.initials: DB
authors.lastname: Gophane
authors.forename: Snorri Th
authors.initials: ST
authors.lastname: Sigurdsson
mesh_terms: D003562:Cytidine / Q000031:analogs & derivatives / Q000737:chemistry*; D015394:Molecular Structure; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D013113:Spin Labels; D013237:Stereoisomerism; D013816:Thermodynamics
chemical_list: D013113:Spin Labels; D003562:Cytidine; D012313:RNA