title: Aminonaphthalene 2-cyanoacrylate (ANCA) probes fluorescently discriminate between amyloid-β and prion plaques in brain.
abstract: A major challenge for diagnosing and monitoring the progression of amyloid-based diseases is the capability to distinguish between amyloid deposits that are associated with related, but distinctly different, diseases. Here, we demonstrate that aminonaphthalenyl 2-cyanoacrylate-based probes can fluorescently discriminate between different types of amyloid deposits in brain. The discriminating capability of these molecular rotors is due to the stabilization of the ground versus excited states of these probes as a function of the polarity of their microenvironment (i.e., within the binding pocket on the amyloid). This property makes it possible, for the first time, to estimate the inherent static relative permittivity (ε(0)) of the binding pocket of each amyloid within tissue. The capability to selectively follow the deposition of specific amyloids in tissue may provide important information for therapeutic development that is not readily accessible from currently available technology.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093-0358, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Cao
authors.forename: Mona
authors.initials: M
authors.lastname: Farahi
authors.forename: Marianna
authors.initials: M
authors.lastname: Dakanali
authors.forename: Willy M
authors.initials: WM
authors.lastname: Chang
authors.forename: Christina J
authors.initials: CJ
authors.lastname: Sigurdson
authors.forename: Emmanuel A
authors.initials: EA
authors.lastname: Theodorakis
authors.forename: Jerry
authors.initials: J
authors.lastname: Yang
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D000818:Animals; D001921:Brain / Q000473:pathology; D003487:Cyanoacrylates / Q000737:chemistry*; D004195:Disease Models, Animal; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D051379:Mice; D015394:Molecular Structure; D009281:Naphthalenes / Q000737:chemistry*; D058225:Plaque, Amyloid / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D003487:Cyanoacrylates; D005456:Fluorescent Dyes; D009281:Naphthalenes