title: Inhibitory effect on in vitro Streptococcus oralis biofilm of a soda-lime glass containing silver nanoparticles coating on titanium alloy.
abstract: This paper reports the effect of soda-lime-glass-nAg coating on the viability of an in vitro biofilm of Streptococcus oralis. Three strains (ATCC 35037 and two clinical isolates from periodontitis patients) were grown on coated with glass, glass containing silver nanoparticles, and uncoated titanium alloy disks. Two different methods were used to quantify biofilm formation abilities: crystal violet staining and determination of viable counts. The influence of the surface morphology on the cell attachment was studied. The surface morphology was characterized by scanning electron microscopy (SEM) and using a profilometer. SEM was also used to study the formation and the development of biofilm on the coated and uncoated disks. At least a >99.7% inocula reduction of biofilm respect to titanium disks and also to glass coated disks was observed in the glass-nAg coated disks for all the studied strains. A quantitative evaluation of the release of silver was conducted in vitro to test whether and to what extend the biocidal agent (silver) could leach from the coating. These findings suggest that the biofilm formation of S. oralis strains is highly inhibited by the glass-nAg and may be useful for materials which require durable antibacterial effect on their surfaces, as it is the case of dental implants.
authors.affiliation: Nanomaterials and Nanotechnology Research Center-Spanish National Research Council-Universidad de Oviedo-Principado de Asturias, Parque Tecnológico de Asturias, Llanera, Spain. b.cabal@cinn.es
authors.forename: Belén
authors.initials: B
authors.lastname: Cabal
authors.forename: Fabio
authors.initials: F
authors.lastname: Cafini
authors.forename: Leticia
authors.initials: L
authors.lastname: Esteban-Tejeda
authors.forename: Luís
authors.initials: L
authors.lastname: Alou
authors.forename: José F
authors.initials: JF
authors.lastname: Bartolomé
authors.forename: David
authors.initials: D
authors.lastname: Sevillano
authors.forename: Roberto
authors.initials: R
authors.lastname: López-Piriz
authors.forename: Ramón
authors.initials: R
authors.lastname: Torrecillas
authors.forename: José Serafín
authors.initials: JS
authors.lastname: Moya
mesh_terms: D000497:Alloys; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D017610:Calcium Compounds / Q000494:pharmacology*; D005840:Gentian Violet; D005898:Glass / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000494:pharmacology*; D010933:Plankton / Q000187:drug effects; D012834:Silver / Q000494:pharmacology*; D012972:Sodium Hydroxide / Q000494:pharmacology*; D013194:Staining and Labeling; D019533:Streptococcus oralis / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D013499:Surface Properties; D013696:Temperature; D014025:Titanium / Q000494:pharmacology*
chemical_list: D000497:Alloys; D017610:Calcium Compounds; D010087:Oxides; C031462:titanium alloy (TiAl6V4); D012834:Silver; C004569:soda lime; D012972:Sodium Hydroxide; D014025:Titanium; D005840:Gentian Violet