title: Highly conserved protective epitopes on influenza B viruses.
abstract: Identification of broadly neutralizing antibodies against influenza A viruses has raised hopes for the development of monoclonal antibody-based immunotherapy and "universal" vaccines for influenza. However, a substantial part of the annual flu burden is caused by two cocirculating, antigenically distinct lineages of influenza B viruses. Here, we report human monoclonal antibodies, CR8033, CR8071, and CR9114, that protect mice against lethal challenge from both lineages. Antibodies CR8033 and CR8071 recognize distinct conserved epitopes in the head region of the influenza B hemagglutinin (HA), whereas CR9114 binds a conserved epitope in the HA stem and protects against lethal challenge with influenza A and B viruses. These antibodies may inform on development of monoclonal antibody-based treatments and a universal flu vaccine for all influenza A and B viruses.
authors.affiliation: Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Cyrille
authors.initials: C
authors.lastname: Dreyfus
authors.forename: Nick S
authors.initials: NS
authors.lastname: Laursen
authors.forename: Ted
authors.initials: T
authors.lastname: Kwaks
authors.forename: David
authors.initials: D
authors.lastname: Zuijdgeest
authors.forename: Reza
authors.initials: R
authors.lastname: Khayat
authors.forename: Damian C
authors.initials: DC
authors.lastname: Ekiert
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Metlagel
authors.forename: Miriam V
authors.initials: MV
authors.lastname: Bujny
authors.forename: Mandy
authors.initials: M
authors.lastname: Jongeneelen
authors.forename: Remko
authors.initials: R
authors.lastname: van der Vlugt
authors.forename: Mohammed
authors.initials: M
authors.lastname: Lamrani
authors.forename: Hans J W M
authors.initials: HJ
authors.lastname: Korse
authors.forename: Eric
authors.initials: E
authors.lastname: Geelen
authors.forename: Özcan
authors.initials: Ö
authors.lastname: Sahin
authors.forename: Martijn
authors.initials: M
authors.lastname: Sieuwerts
authors.forename: Just P J
authors.initials: JP
authors.lastname: Brakenhoff
authors.forename: Ronald
authors.initials: R
authors.lastname: Vogels
authors.forename: Olive T W
authors.initials: OT
authors.lastname: Li
authors.forename: Leo L M
authors.initials: LL
authors.lastname: Poon
authors.forename: Malik
authors.initials: M
authors.lastname: Peiris
authors.forename: Wouter
authors.initials: W
authors.lastname: Koudstaal
authors.forename: Andrew B
authors.initials: AB
authors.lastname: Ward
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
authors.forename: Jaap
authors.initials: J
authors.lastname: Goudsmit
authors.forename: Robert H E
authors.initials: RH
authors.lastname: Friesen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D057134:Antibodies, Neutralizing / Q000737:chemistry / Q000276:immunology; D017124:Conserved Sequence; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology*; D009981:Influenza B virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009976:Orthomyxoviridae Infections / Q000517:prevention & control*; D011487:Protein Conformation
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D016056:Immunodominant Epitopes; D007252:Influenza Vaccines; C493831:hemagglutinin HA1, influenza B virus