title: Isolation of marine bacteria highly resistant to mercury and their bioaccumulation process.
abstract: The marine bacteria strains S1, S2 and S3, were isolated on seawater culturing medium containing Hg(2+), Cd(2+), Cr(6+) or Ni(2+) at concentrations of 20 mg L(-1) and more. The isolates showed tolerance to these heavy metals. S1 grew in the presence of 120 mg L(-1) of Hg(2+) and accumulated Hg(2+) at pH 4-10. For the effect of co-existing cations on bioaccumulation of target metal, it was found that the effect depended on not only the variety and initial concentration of co-existing ions, but also the initial concentration of target metal and biomass dosage. A new parameter, the ratio of remaining bioaccumulation capacity (RRBC) of biomass, was therefore defined to evaluate such effect. Furthermore, the mechanism evaluation revealed that S1 bound about 70% Hg(2+) on the cell surface, and carboxyl group played an important role in Hg(2+) binding.
authors.affiliation: College of Life Sciences, Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, Shenzhen 518060, PR China. dengxu@szu.edu.cn
authors.forename: Xu
authors.initials: X
authors.lastname: Deng
authors.forename: Pengting
authors.initials: P
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000378:metabolism; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008628:Mercury / Q000493:pharmacokinetics* / Q000633:toxicity; D010802:Phylogeny; D012623:Seawater / Q000382:microbiology*; D013045:Species Specificity; D013057:Spectrum Analysis; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics* / Q000633:toxicity; D014876:Water Pollution / Q000517:prevention & control*
chemical_list: D017931:DNA Primers; D014874:Water Pollutants, Chemical; D002245:Carbon Dioxide; C041069:carboxyl radical; D008628:Mercury