title: Intermittent hydrostatic pressure enhances growth factor-induced chondroinduction of human adipose-derived mesenchymal stem cells.
abstract: Hydrostatic pressure (HP) plays an essential role in regulating function of chondrocytes and chondrogenic differentiation. The objective of this study was to examine effects of intermittent HP on chondrogenic differentiation of human adipose-derived mesenchymal stem cells (hASCs) in the presence or absence of chemical chondrogenic medium. Cells were isolated from abdominal fat tissue and confirmed for expression of ASC surface proteins and differentiation potential. Passage 3 pellets were treated with chemical (growth factor), mechanical (HP of 5 MPa and 0.5 Hz with duration of 4 h/day for 7 consecutive days), and combined chemical-mechanical stimuli. Using real-time polymerase chain reaction, the expression of Sox9, collagen II, and aggrecan as three major chondrogenic markers were quantified among three experimental groups and compared to those of stem cells and human cartilage tissue. In comparison to the chemical and mechanical groups, the chemical-mechanical group showed the highest expression for all three chondrogenic genes close to that of cartilage tissue. Results show the beneficial role of intermittent HP on chondrogenic differentiation of hASCs, and that this loading regime in combination with chondrogenic medium can be used in cartilage tissue engineering.
authors.affiliation: Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Safshekan
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tafazzoli-Shadpour
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.forename: Nooshin
authors.initials: N
authors.lastname: Haghighipour
authors.forename: Reza
authors.initials: R
authors.lastname: Mahdian
authors.forename: Alireza
authors.initials: A
authors.lastname: Hemmati
mesh_terms: D050153:Abdominal Fat / Q000166:cytology*; D053674:Aggrecans / Q000378:metabolism; D002454:Cell Differentiation; D020219:Chondrogenesis / Q000235:genetics; D024043:Collagen Type II / Q000378:metabolism; D003470:Culture Media; D015870:Gene Expression; D006801:Humans; D006874:Hydrostatic Pressure; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D016133:Polymerase Chain Reaction; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism; D023822:Tissue Engineering
chemical_list: D053674:Aggrecans; D024043:Collagen Type II; D003470:Culture Media; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human