title: Calibration and validation scheme for in vivo spectroscopic imaging of tissue oxygenation.
abstract: The determination of the level of oxygenation in optically accessible tissues using multispectral or hyperspectral imaging (HSI) of oxy- and deoxyhemoglobin has special appeal in clinical work due to its noninvasiveness, ease of use, and capability of providing molecular and anatomical information at near video rates during surgery. In this paper we refer to an example of the use of HSI in monitoring oxygenation of kidneys during partial nephrectomy. In a study using porcine models, it was found that artery-only clamping left the kidney better oxygenated, as opposed to simultaneously clamping the artery and the vein. A subsequent study correlates gradations in blood flow by partial clamping during the surgical procedure with postoperative renal function via assessment of creatinine level. We discuss the various contributions to the uncertainty of the oxygen saturation measured by this remote-sensing imaging technique in medical application.
authors.affiliation: National Institute of Standards and Technology, 100 Bureau Dr. Stop 8443, Gaithersburg, MD, 20899, USA. litorja@nist.gov.
authors.forename: Maritoni
authors.initials: M
authors.lastname: Litorja
authors.affiliation: National Institute of Standards and Technology, 100 Bureau Dr. Stop 8443, Gaithersburg, MD, 20899, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Chang
authors.affiliation: National Institute of Standards and Technology, 100 Bureau Dr. Stop 8443, Gaithersburg, MD, 20899, USA.
authors.forename: Jeeseong
authors.initials: J
authors.lastname: Hwang
authors.affiliation: National Institute of Standards and Technology, 100 Bureau Dr. Stop 8443, Gaithersburg, MD, 20899, USA.
authors.forename: David W
authors.initials: DW
authors.lastname: Allen
authors.affiliation: Digital Light Innovations, Dallas, TX, USA.
authors.forename: Karel
authors.initials: K
authors.lastname: Zuzak
authors.affiliation: University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Eleanor
authors.initials: E
authors.lastname: Wehner
authors.affiliation: University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Best
authors.affiliation: University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Edward
authors.initials: E
authors.lastname: Livingston
authors.affiliation: University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Cadeddu
mesh_terms: D000818:Animals; D002138:Calibration; D003250:Constriction; D003404:Creatinine / Q000097:blood; D006454:Hemoglobins / Q000378:metabolism; D007511:Ischemia / Q000473:pathology* / Q000601:surgery; D007668:Kidney / Q000098:blood supply* / Q000473:pathology* / Q000601:surgery; D009392:Nephrectomy; D010100:Oxygen / Q000097:blood*; D012077:Renal Artery / Q000473:pathology / Q000601:surgery; D013057:Spectrum Analysis; D013552:Swine
chemical_list: D006454:Hemoglobins; C100003:deoxyhemoglobin; D003404:Creatinine; D010100:Oxygen