title: Inhibition of platelet aggregation by chlorogenic acid via cAMP and cGMP-dependent manner.
abstract: In this study, we investigated the effect of chlorogenic acid, a phenolic acid, on collagen (10 μg/ml)-stimulated platelet aggregation. Chlorogenic acid dose-dependently inhibited collagen-induced platelet aggregation, and suppressed the production of thromboxane A2 (TXA2), an intracellular Ca-agonist as an aggregation-inducing autacoidal molecule, which was associated with the strong inhibition of cyclooxygenase (COX)-1 in platelet microsomes having cytochrome c reductase activity. In addition, chlorogenic acid increased significantly the formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), intracellular Ca-antagonists as aggregation-inhibiting molecules. These results suggest that chlorogenic acid has antiplatelet activity through the reduction of TXA2 and the increase of cAMP and cGMP levels. Therefore, our data demonstrate that chlorogenic acid is a potent inhibitor of collagen-stimulated platelet aggregation, and may be a crucial tool for a negative regulator during platelet activation in thrombotic diseases.
authors.affiliation: Department of Biomedical Laboratory Science, College of Medical Science, Konyang University, Gasuwon-dong, Seo-gu, Daejeon, Republic of Korea.
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Cho
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Kang
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Dong-Ha
authors.initials: DH
authors.lastname: Lee
authors.forename: Hyuk-Woo
authors.initials: HW
authors.lastname: Kwon
authors.forename: Yun-Yi
authors.initials: YY
authors.lastname: Kim
authors.forename: Hwa-Jin
authors.initials: HJ
authors.lastname: Park
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D002726:Chlorogenic Acid / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis / Q000097:blood*; D006152:Cyclic GMP / Q000096:biosynthesis / Q000097:blood*; D006801:Humans; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013928:Thromboxane A2 / Q000096:biosynthesis / Q000097:blood
chemical_list: D010975:Platelet Aggregation Inhibitors; D002726:Chlorogenic Acid; D013928:Thromboxane A2; D003094:Collagen; D000242:Cyclic AMP; D006152:Cyclic GMP