title: Microfabricated nanotopological surfaces for study of adhesion-dependent cell mechanosensitivity.
abstract: Cells exhibit high sensitivity and diverse responses to the intrinsic nanotopography of the extracellular matrix through their nanoscale cellular sensing machinery. A simple microfabrication method for precise control and spatial patterning of the local nanoroughness on glass surfaces by using photolithography and reactive ion etching is reported. It is demonstrated that local nanoroughness as a biophysical cue could regulate a diverse array of NIH/3T3 fibroblast behaviors, including cell morphology, adhesion, proliferation, migration, and cytoskeleton contractility. The capability to control and further predict cellular responses to nanoroughness might suggest novel methods for developing biomaterials mimicking nanotopographic structures in vivo for functional tissue engineering.
authors.affiliation: Integrated Biosystems and Biomechanics Laboratory, Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48105, USA.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Chen
authors.forename: Yubing
authors.initials: Y
authors.lastname: Sun
authors.forename: Jianping
authors.initials: J
authors.lastname: Fu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001703:Biophysics / Q000379:methods; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement; D049109:Cell Proliferation; D003599:Cytoskeleton / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D051379:Mice; D018625:Microscopy, Atomic Force / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000379:methods; D041681:NIH 3T3 Cells; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D023822:Tissue Engineering / Q000379:methods
chemical_list: D001672:Biocompatible Materials