title: Purification, crystallization and preliminary X-ray analysis of the IgV domain of human nectin-4.
abstract: Nectin-4 belongs to a family of immunoglobulin-like cell adhesion molecules and is highly expressed in cancer cells. Recently, nectin-4 was found to be a receptor of measles virus and the IgV domain sustains strong binding to measles virus H protein. In this study, the successful expression and purification of human nectin-4 V domain (nectin-4v) is reported. The purified protein was crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to 1.8 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.1, b = 51.7, c = 56.9 Å, β = 94.7°. Preliminary analysis of the diffraction data was also performed.
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui 230036, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Xu
authors.forename: Xiaoai
authors.initials: X
authors.lastname: Zhang
authors.forename: Guangwen
authors.initials: G
authors.lastname: Lu
authors.forename: Yongping
authors.initials: Y
authors.lastname: Cai
mesh_terms: D015815:Cell Adhesion Molecules / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D017434:Protein Structure, Tertiary; D000075983:Nectins
chemical_list: D015815:Cell Adhesion Molecules; C442228:NECTIN4 protein, human; D000075983:Nectins