title: DNA binding mode of novel tetradentate amino acid based 2-hydroxybenzylidene-4-aminoantipyrine complexes.
abstract: Few transition metal complexes of tetradentate N(2)O(2) donor Schiff base ligands containing 2-hydroxybenzylidene-4-aminoantipyrine and amino acids (alanine/valine) abbreviated to KHL(1)/KHL(2) have been synthesized. All the metal complexes have been fully characterized with the help of elemental analyses, molecular weights, molar conductance values, magnetic moments and spectroscopic data. The Schiff bases KHL(1)/KHL(2) are found to act as tetradentate ligands using N(2)O(2) donor set of atoms leading to a square-planar geometry for the complexes around the metal ions. The binding behaviors of the complexes to calf thymus DNA have been investigated by absorption spectra, viscosity measurements and cyclic voltammetry. The DNA binding constants reveal that all these complexes interact with DNA through minor groove binding mode. The studies on mechanism of photocleavage reveal that singlet oxygen ((1)O(2)) and superoxide anion radical (O(2)(-)) may play an important role in the photocleavage. The Schiff bases and their metal complexes have been screened for their in vitro antibacterial activities against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis, Klebsiella pneumoniae and antifungal activities against Aspergillus niger, Fusarium solani, Culvularia lunata, Rhizoctonia bataicola and Candida albicans by MIC method.
authors.affiliation: Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, India. drn_raman@yahoo.co.in
authors.forename: N
authors.initials: N
authors.lastname: Raman
authors.forename: S
authors.initials: S
authors.lastname: Sobha
authors.forename: M
authors.initials: M
authors.lastname: Selvaganapathy
authors.forename: R
authors.initials: R
authors.lastname: Mahalakshmi
mesh_terms: D000042:Absorption; D000596:Amino Acids / Q000378:metabolism*; D000675:Ampyrone / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001597:Benzylidene Compounds / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004553:Electric Conductivity; D055664:Electrochemical Techniques; D004578:Electron Spin Resonance Spectroscopy; D004583:Electrons; D004587:Electrophoresis, Agar Gel; D005658:Fungi / Q000187:drug effects; D008024:Ligands; D060328:Magnetic Phenomena; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000378:metabolism; D012545:Schiff Bases / Q000138:chemical synthesis / Q000737:chemistry; D012996:Solutions; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D014783:Viscosity
chemical_list: D000596:Amino Acids; D000890:Anti-Infective Agents; D001597:Benzylidene Compounds; D008024:Ligands; D012545:Schiff Bases; D012996:Solutions; D000675:Ampyrone; D004247:DNA; C427203:calf thymus DNA