title: Optofluidic detection for cellular phenotyping.
abstract: Quantitative analysis of the output of processes and molecular interactions within a single cell is highly critical to the advancement of accurate disease screening and personalized medicine. Optical detection is one of the most broadly adapted measurement methods in biological and clinical assays and serves cellular phenotyping. Recently, microfluidics has obtained increasing attention due to several advantages, such as small sample and reagent volumes, very high throughput, and accurate flow control in the spatial and temporal domains. Optofluidics, which is the attempt to integrate optics with microfluidics, shows great promise to enable on-chip phenotypic measurements with high precision, sensitivity, specificity, and simplicity. This paper reviews the most recent developments of optofluidic technologies for cellular phenotyping optical detection.
authors.affiliation: Research Center for Applied Sciences, Academia Sinica, 128 Sec. 2, Academia Rd. Nankang, Taipei, 11529, Taiwan.
authors.forename: Yi-Chung
authors.initials: YC
authors.lastname: Tung
authors.forename: Nien-Tsu
authors.initials: NT
authors.lastname: Huang
authors.forename: Bo-Ram
authors.initials: BR
authors.lastname: Oh
authors.forename: Bishnubrata
authors.initials: B
authors.lastname: Patra
authors.forename: Chi-Chun
authors.initials: CC
authors.lastname: Pan
authors.forename: Teng
authors.initials: T
authors.lastname: Qiu
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhang
authors.forename: Katsuo
authors.initials: K
authors.lastname: Kurabayashi
mesh_terms: D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D007368:Interferometry; D044085:Microfluidics / Q000295:instrumentation*; D055095:Optics and Photonics / Q000295:instrumentation*; D010641:Phenotype; D057285:Precision Medicine; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman; D020349:Surface Plasmon Resonance