title: The growth of the canine glioblastoma cell line D-GBM and the canine histiocytic sarcoma cell line DH82 is inhibited by the resveratrol oligomers hopeaphenol and r2-viniferin.
abstract: Vineatrol(®) 30 is a grapevine-shoot extract, which contains resveratrol as well as considerable amounts of so-called resveratrol oligomers such as hopeaphenol and r2-viniferin. In this study, we analysed whether the two above-mentioned resveratrol oligomers were able to inhibit the growth of the canine glioblastoma cell line D-GBM and the canine histiocytic sarcoma cell line DH82, compared their potency to inhibit tumour cell growth with that of resveratrol and determined whether the induction of apoptosis via caspase 9 and 3/7 activation underlies the tumour cell growth-inhibiting effect of hopeaphenol and r2-viniferin. Vineatrol(®) 30, resveratrol, hopeaphenol and r2-viniferin inhibited the growth of D-GBM and DH82 cells in a concentration-dependent manner, whereby hopeaphenol and r2-viniferin were more potent than resveratrol itself in inhibiting the growth of the canine tumour cell lines. Moreover, the anti-proliferative effect of both resveratrol oligomers in D-GBM cells is based on their capacity to induce caspase 9 and 3/7 activation.
authors.affiliation: Institute for Food Toxicology and Analytical Chemistry, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: M T
authors.initials: MT
authors.lastname: Empl
authors.forename: S
authors.initials: S
authors.lastname: Macke
authors.forename: P
authors.initials: P
authors.lastname: Winterhalter
authors.forename: C
authors.initials: C
authors.lastname: Puff
authors.forename: S
authors.initials: S
authors.lastname: Lapp
authors.forename: G
authors.initials: G
authors.lastname: Stoica
authors.forename: W
authors.initials: W
authors.lastname: Baumgärtner
authors.forename: P
authors.initials: P
authors.lastname: Steinberg
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D004285:Dogs; D005909:Glioblastoma / Q000378:metabolism*; D054747:Histiocytic Sarcoma / Q000378:metabolism*; D015394:Molecular Structure; D010636:Phenols; D059808:Polyphenols / Q000737:chemistry* / Q000494:pharmacology; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D010636:Phenols; D059808:Polyphenols; D013267:Stilbenes; C518233:hopeaphenol; C000593370:r2-viniferin; D000077185:Resveratrol