title: In vitro and in vivo antitumor activity of doxorubicin-loaded alginic-acid-based nanoparticles.
abstract: The antitumor activities of DOX-loaded alginic acid/poly[2-(diethylamino)ethyl methacrylate] (ALG-PDEA) nanoparticles are evaluated both in vitro and in vivo. TEM imaging shows that the ALG-PDEA NPs have a spherical morphology with a size of about 120 nm. CLSM observations reveal that the negatively charged ALG-PDEA NPs can be taken up well by cells. In vivo NIR fluorescence imaging shows that the ALG-PDEA NPs can passively target the tumor area because of the EPR effect in the H22 tumor-bearing mouse. In vivo antitumor efficacy examinations indicate that DOX-loaded ALG-PDEA NPs have significantly superior efficacy in impeding tumor growth compared to free DOX and low toxicity to living mice.
authors.affiliation: Laboratory of Mesoscopic Chemistry and Department of Polymer Science and Engineering, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cheng
authors.forename: Shuling
authors.initials: S
authors.lastname: Yu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.forename: Hanqing
authors.initials: H
authors.lastname: Qian
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Xiqun
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000464:Alginates; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D001692:Biological Transport; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D004337:Drug Carriers; D004339:Drug Compounding; D005456:Fluorescent Dyes; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D053758:Nanoparticles; D009368:Neoplasm Transplantation; D061848:Optical Imaging; D010316:Particle Size; D011109:Polymethacrylic Acids
chemical_list: D000464:Alginates; D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D005456:Fluorescent Dyes; D011109:Polymethacrylic Acids; C520965:alginic acid-poly(2-(diethylamino)ethyl methacrylate); D004317:Doxorubicin