title: Exploration of a possible partnership among orphan two-component system proteins in cyanobacterium Synechococcus elongatus PCC 7942.
abstract: To understand the induction of the adaptive response under various stress conditions, it is important to determine the partnership between histidine kinase and response regulators in the bacterial two-component system (TCS). The genes encoding TCS partners are usually comprised of an operon in the genome, but many of them are orphans in the cyanobacterial genome. There is little information on their partnerships in Synechococcus elongatus PCC 7942. Our comprehensive analysis of protein-protein interactions among all 37 full-length proteins and the truncated domains of 24 orphans revealed a number of specific interactions. They involved evolutionarily well-conserved orphan proteins among cyanobacterial species such as Synpcc7942_0453/Ycf29, NblS/RpaB, NblS/SrrA, SasA/RpaA, and SasA/Synpcc7942_2466. Our investigation of the transphosphorylation of interaction partners indicates that orphan TCSs comprise a complex signaling network.
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Tokyo 156-8502, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kato
authors.forename: Satoru
authors.initials: S
authors.lastname: Watanabe
authors.forename: Kaori
authors.initials: K
authors.lastname: Nimura-Matsune
authors.forename: Taku
authors.initials: T
authors.lastname: Chibazakura
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Tozawa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D000222:Adaptation, Physiological; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D000071677:Histidine Kinase; D009876:Operon; D010766:Phosphorylation; D025941:Protein Interaction Mapping; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D015398:Signal Transduction; D013312:Stress, Physiological; D046940:Synechococcus / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001426:Bacterial Proteins; D011494:Protein Kinases; D000071677:Histidine Kinase