title: Involvement of Fenneropenaeus chinensis Cathepsin C in antiviral immunity.
abstract: Cathepsin C (Cath C) is a lysosomal cysteine protease that belongs to the papain superfamily. Cath C is capable of activating many chymotrypsin-like serine proteases and is reported to be a central coordinator for the activation of many serine proteinases in immune and inflammatory cells. In this study, Cath C cDNA was cloned from Fenneropenaeus chinensis (Fc). The complete cDNA of Fc-Cath C in Chinese white shrimp was found to be 1445-base pairs (bp) long. It contained an open reading frame (ORF) 1356-bp long and encoded a 451-amino acid residue protein, including a 17-amino acid residue signal peptide. Real-time PCR analysis results indicated that Fc-Cath C was present in all the tissues detected and exhibited high level of transcription in the hepatopancreas. In hemocytes, hepatopancreas, gills and intestine, Fc-Cath C was upregulated after stimulation by the Vibrio anguillarum and the white spot syndrome viruses (WSSVs). Replication of the WSSV increased after the injection of Fc-Cath C antiserum or knockdown Cath C by RNA interference. These results implied that Cath C might play a crucial role in the antiviral immune response of shrimp.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of Ministry of Education, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Shi
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: An-Jing
authors.initials: AJ
authors.lastname: Chen
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Zhao
authors.forename: Jin-Xing
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D020789:Cathepsin C / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D007274:Injections, Intraperitoneal / Q000662:veterinary; D008969:Molecular Sequence Data; D009928:Organ Specificity; D033561:Penaeidae / Q000235:genetics / Q000276:immunology* / Q000821:virology; D010802:Phylogeny; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D014733:Vibrio / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000276:immunology
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D011994:Recombinant Proteins; D020789:Cathepsin C