title: miR-203 inhibits proliferation of HCC cells by targeting survivin.
abstract: To validate whether down-regulation of microRNA-203 (miR-203) in hepatocellular carcinoma (HCC) is involved in HCC progression by targeting survivin. MiR-203 mimics was transfected into HepG2 cells to enhance miR-203 expression, and miR-203 inhibitor was transfected into HepG2 cells to inhibit miR-203 expression. The effect of up-regulation and down-regulation of miR-203 on survivin expression of HepG2 cells was evaluated using Western blot assay. The effect of miR-203 or survivin expression on the proliferation of HepG2 cells was detected using the CKK-8 assay. Over-expression of miR-203 significantly inhibited the expression of survivin in HepG2 cells (p < 0·05), and down-expression of miR-203 significantly promoted the expression of survivin in HepG2 cells (p < 0·05). Both over-expression of miR-203 and down-regulation of survivin suppressed proliferation of HepG2 cells significantly compared with negative control. Low expression of miR-203 contributes to the progression of HCC via targeting survivin.
authors.affiliation: Department of General Surgery, China-Japan Union Hospital, Jilin University, Changchun, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Wei
authors.forename: Liu
authors.initials: L
authors.lastname: Wanjun
authors.forename: Sun
authors.initials: S
authors.lastname: Hui
authors.forename: Chen
authors.initials: C
authors.lastname: Dongyue
authors.forename: Yao
authors.initials: Y
authors.lastname: Xinjun
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jisheng
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002455:Cell Division; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D008113:Liver Neoplasms / Q000473:pathology*; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000502:physiology*; D009363:Neoplasm Proteins / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000502:physiology*; D034741:RNA, Small Interfering / Q000494:pharmacology; D000077022:Survivin; D014162:Transfection; D015854:Up-Regulation
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D034741:RNA, Small Interfering; D000077022:Survivin