title: A high-throughput forward genetic screen identifies genes required for virulence of Pseudomonas syringae pv. maculicola ES4326 on Arabidopsis.
abstract: Successful pathogenesis requires a number of coordinated processes whose genetic bases remain to be fully characterized. We utilized a high-throughput, liquid media-based assay to screen transposon disruptants of the phytopathogen Pseudomonas syringae pv. maculicola ES4326 to identify genes required for virulence on Arabidopsis. Many genes identified through this screen were involved in processes such as type III secretion, periplasmic glucan biosynthesis, flagellar motility, and amino acid biosynthesis. A small set of genes did not fall into any of these functional groups, and their disruption resulted in context-specific effects on in planta bacterial growth.
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Karl J
authors.initials: KJ
authors.lastname: Schreiber
authors.forename: David
authors.initials: D
authors.lastname: Ye
authors.forename: Eric
authors.initials: E
authors.lastname: Fich
authors.forename: Allen
authors.initials: A
authors.lastname: Jian
authors.forename: Timothy
authors.initials: T
authors.lastname: Lo
authors.forename: Darrell
authors.initials: D
authors.lastname: Desveaux
mesh_terms: D017360:Arabidopsis / Q000382:microbiology*; D004251:DNA Transposable Elements / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D044224:Pseudomonas syringae / Q000235:genetics* / Q000472:pathogenicity*; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D037521:Virulence Factors