title: [Association between amino acid mutations in Gag protein and human leukocyte antigen class I alleles in human immunodeficiency virus-1 B' infected Han Chinese people].
abstract: OBJECTIVE
  To explore the human leukocyte antigen (HLA)-associated mutations in Gag protein of B' clade (human immunodeficiency virus-1) HIV-1 infected Han Chinese people and evaluate the impact of HLA associated Gag mutations on the disease progression of HIV infection.
  
  
  METHODS
  A total of 95 B' clade HIV-1 infected Han Chinese cases were recruited. The gag sequences were amplified from viral RNA and sequenced directly. HLA-I genotypes were detected with the assay of polymerase chain reaction-sequence specific primer (PCR-SSP). HLA-associated mutations were identified and the relationships between HLA-associated mutations and CD4+ T cell counts or plasma viral loads analyzed.
  
  
  RESULTS
  Forty-seven kinds of mutations at 28 sites (15, 18, 26, 30, 34, 46, 62, 67, 81, 84, 90, 102, 118, 121, 122, 125, 146, 147, 173, 176, 252, 357, 374, 376, 437, 470, 471, 478) of Gag protein were significantly associated with particular HLA class I allelotypes (P < 0.05). Among which, 9 sites (26, 30, 81, 84, 125, 146, 147, 357, 437) were located within 13 known cytotoxic T-lymphocyte (CTL) epitopes or flanking regions. The number of HLA-associated mutations was significantly associated with both CD4 T cell counts (r = -0.318, P = 0.002) and viral loads (r = 0.360, P = 0.003).
  
  
  CONCLUSIONS
  HLA-associated mutations may have a significant impact on HIV disease progression in B' clade HIV-1 infected Han Chinese population.
authors.affiliation: Key Laboratory of AIDS Immunology of Ministry of Health, Department of Laboratory Medicine, First Hospital, China Medical University, Shenyang 110001, China.
authors.forename: Xiao-xu
authors.initials: XX
authors.lastname: Han
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Ou
authors.initials: O
authors.lastname: Chen
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.forename: Yang-tao
authors.initials: YT
authors.lastname: Ji
authors.forename: Zi-ning
authors.initials: ZN
authors.lastname: Zhang
authors.forename: Wei-guo
authors.initials: WG
authors.lastname: Cui
authors.forename: Bao-gui
authors.initials: BG
authors.lastname: Liu
authors.forename: Song
authors.initials: S
authors.lastname: Duan
authors.forename: Hong
authors.initials: H
authors.lastname: Shang
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics* / Q000821:virology*; D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D005260:Female; D005805:Genes, MHC Class I; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D012367:RNA, Viral; D019562:Viral Load; D055815:Young Adult; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D054301:gag Gene Products, Human Immunodeficiency Virus