title: Effect of furanone on experimentally induced Pseudomonas aeruginosa biofilm formation: in vitro study.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the effect of furanone as a quorum sensing inhibitor with an antibiotic agent on experimentally induced Pseudomonas aeruginosa biofilm formation in an in vitro model.
  
  
  METHODS
  Bacterial cultures on agar plates and induction of biofilm formations were done in four different conditions; P. aeruginosa only (group 1), P. aeruginosa+ciprofloxacin (group 2), P. aeruginosa+furanone (group 3), and P. aeruginosa+furanone+ciprofloxacin (group 4). After biofilm formation on a silicone tympanostomy tube with P. aeruginosa was established, they were treated with ciprofloxacin, furanone, and ciprofloxacin with furanone respectively. All agar plates were processed for colony counts 1 day after incubation, and silicone tympanostomy tubes were observed using scanning electron microscopy 2 days after incubation. Any changes visualized on the biofilm lining the silicone tympanostomy tubes after treatment initiation were evaluated again 2 days after each treatment.
  
  
  RESULTS
  There was no bacterial growth on the agar plate in group 4 compared to other groups and the formations of a biofilm on the surface of silicone tympanostomy tubes in group 4 were devoid. Little change to the original biofilm overlying the silicone tympanostomy tubes after each treatment was noted.
  
  
  CONCLUSIONS
  This study demonstrates that furanone may have a role as a QSI and can be helpful in preventing biofilm formation with ciprofloxacin in experimentally induced P. aeruginosa biofilms based on an in vitro model.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, College of Medicine Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Sun Gui
authors.initials: SG
authors.lastname: Kim
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Yoon
authors.forename: Jin Woong
authors.initials: JW
authors.lastname: Choi
authors.forename: Ki Sang
authors.initials: KS
authors.lastname: Rha
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Park
mesh_terms: D000362:Agar; D000890:Anti-Infective Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002939:Ciprofloxacin / Q000494:pharmacology; D015169:Colony Count, Microbial; D003470:Culture Media; D005663:Furans / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D008876:Middle Ear Ventilation / Q000295:instrumentation; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media; D005663:Furans; C494198:furanone C-30; D002939:Ciprofloxacin; D000362:Agar