title: Simultaneous electrochemical immunoassay using CdS/DNA and PbS/DNA nanochains as labels.
abstract: An electrochemical method for the simultaneous detection of two different tumor markers, carcinoembryonic antigen (CEA) and α-fetoprotein (AFP), in one-pot, using CdS/DNA and PbS/DNA nanochains as labels was developed. Herein, magnetic beads (MBs) as bimolecule immobilizing carriers, were used for co-immobilization of primary anti-CEA and anti-AFP antibodies. The distinguishable signal labels were synthesized by in situ growth of CdS and PbS nanoparticles on DNA chains, respectively, which were further employed to label the corresponding secondary antibodies. A sandwich-type immunoassay format was formed by the biorecognition of the antigens and corresponding antibodies. The assay was based on the peak currents of Cd(2+) and Pb(2+) dissolved from CdS and PbS nanoparticles by HNO(3) using square wave stripping voltammetry. Experimental results show that the multiplexed electrochemical immunoassay has enabled the simultaneous monitoring of CEA and AFP in a single run with wide working ranges of 0.1-100ng mL(-1) for CEA and 0.5-200ng mL(-1) for AFP. The detection limits reach to 3.3pg mL(-1) for CEA and 7.8pg mL(-1) for AFP.
authors.affiliation: The State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210093, China.
authors.forename: Fen-Ying
authors.initials: FY
authors.lastname: Kong
authors.forename: Bi-Yi
authors.initials: BY
authors.lastname: Xu
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis / Q000276:immunology; D019187:Cadmium Compounds / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000276:immunology; D004247:DNA / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007854:Lead / Q000737:chemistry*; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D018036:Selenium Compounds / Q000737:chemistry*; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
chemical_list: D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D019187:Cadmium Compounds; D002272:Carcinoembryonic Antigen; D018036:Selenium Compounds; D000509:alpha-Fetoproteins; C088065:lead selenide; D007854:Lead; D004247:DNA; C058667:cadmium selenide