title: Characterisation and partial purification of proteolytic enzymes from sardine by-products to obtain concentrated hydrolysates.
abstract: A procedure to recover proteases and lipases from the by-products of Monterey sardine (Sardinops sagax caerulea) has been developed, comprising 2 steps: a centrifugation at low temperature to eliminate more than 90% of the initial fat content, and an acetone precipitation step. After this treatment, enzymatic activity increased by 33.8% for lipase, 15.5% for trypsin, 14.8% for chymotrypsin, 93.4% for aminopeptidase, and 19.7% for pepsin. The extents of hydrolysis of fish by-product proteins by endogenous enzyme by-product extract, viscera concentrate extract, and commercial Alcalase® were 62%, 85%, and 28%, respectively. The two extract preparations from sardine by-product (viscera and by-product concentrate extracts) produced 3-fold greater hydrolysis than with the commercial enzyme. The recovery of enzyme concentrates from sardine waste has both ecological and economical advantages for the fish industry.
authors.affiliation: Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada a Tijuana 3918, Zona Playitas, CP 22860 Ensenada, Baja California, Mexico.
authors.forename: Ana Bertha
authors.initials: AB
authors.lastname: Castro-Ceseña
authors.forename: M
authors.initials: M
authors.lastname: del Pilar Sánchez-Saavedra
authors.forename: Facundo J
authors.initials: FJ
authors.lastname: Márquez-Rocha
mesh_terms: D000818:Animals; D029941:Fish Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D005399:Fishes; D006868:Hydrolysis; D007220:Industrial Waste / Q000032:analysis; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000302:isolation & purification*; D011492:Protein Hydrolysates / Q000737:chemistry*
chemical_list: D029941:Fish Proteins; D007220:Industrial Waste; D011492:Protein Hydrolysates; D010447:Peptide Hydrolases