title: Simultaneous determination of 15 aminoglycoside(s) residues in animal derived foods by automated solid-phase extraction and liquid chromatography-tandem mass spectrometry.
abstract: An automated method has been developed for the simultaneous quantification of 15 aminoglycosides in muscle, liver (pigs, chicken and cattle), kidney (pigs and cattle), cow milk, and hen eggs by liquid chromatography tandem mass spectrometry. Homogenized samples were extracted by monopotassium phosphate buffer (including ethylene diamine tetraacetic acid), and cleaned up with auto solid-phase extraction by carboxylic acid cartridges. The analytes were separated by a specialized column for aminoglycosides, and eluted with trifluoroacetic acid and acetonitrile. The decision limits (CCα) of apramycin, gentamycin, tobramycin, paromomycin, hygromycin, neomycin, kanamycin, sisomicin, netilmicin, ribostamycin, kasugamycin, amikacin, streptomycin, dihydrostreptomycin and spectinomycin were ranged from 8.1 to 11.8 μg/kg and detection capabilities (CCβ) from 16.4 to 21.8 μg/kg. High correlation coefficients (r(2)>0.99) of calibration curves for the analytes were obtained within linear from 20 to 1000 μg/kg. Reasonable recoveries (71-108%) were demonstrated with excellent relative standard deviation (RSD). This method is simple pretreatment, rapid determination and high sensitivity, which can be used in the determination of multi-aminoglycosides in complex samples.
authors.affiliation: National Reference Laboratory of Veterinary Drug Residues (HZAU)/MOA, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Tao
authors.forename: Dongmei
authors.initials: D
authors.lastname: Chen
authors.forename: Huan
authors.initials: H
authors.lastname: Yu
authors.forename: Lingli
authors.initials: L
authors.lastname: Huang
authors.forename: Zhaoying
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Cao
authors.forename: Caixia
authors.initials: C
authors.lastname: Yan
authors.forename: Yuanhu
authors.initials: Y
authors.lastname: Pan
authors.forename: Zhenli
authors.initials: Z
authors.lastname: Liu
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000617:Aminoglycosides / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000302:isolation & purification; D002417:Cattle; D002645:Chickens; D004350:Drug Residues / Q000032:analysis* / Q000302:isolation & purification; D004531:Eggs / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents