title: Interactions between Clostridium beijerinckii and Geobacter metallireducens in co-culture fermentation with anthrahydroquinone-2, 6-disulfonate (AH2QDS) for enhanced biohydrogen production from xylose.
abstract: To enhance biohydrogen production, Clostridium beijerinckii was co-cultured with Geobacter metallireducens in the presence of the reduced extracellular electron shuttle anthrahydroquinone-2, 6-disulfonate (AH(2)QDS). In the co-culture system, increases of up to 52.3% for maximum cumulative hydrogen production, 38.4% for specific hydrogen production rate, 15.4% for substrate utilization rate, 39.0% for substrate utilization extent, and 34.8% for hydrogen molar yield in co-culture fermentation were observed compared to a pure culture of C. beijerinckii without AH(2)QDS. G. metallireducens grew in the co-culture system, resulting in a decrease in acetate concentration under co-culture conditions and a presumed regeneration of AH(2)QDS from AQDS. These co-culture results demonstrate metabolic crosstalk between the fermentative bacterium C. beijerinckii and the respiratory bacterium G. metallireducens and suggest a strategy for industrial biohydrogen production.
authors.affiliation: Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ye
authors.forename: Kevin T
authors.initials: KT
authors.lastname: Finneran
authors.forename: Julie L
authors.initials: JL
authors.lastname: Zilles
authors.forename: Eberhard
authors.initials: E
authors.lastname: Morgenroth
mesh_terms: D000085:Acetates / Q000378:metabolism; D000873:Anthracenes / Q000378:metabolism*; D056804:Biofuels; D046970:Clostridium beijerinckii / Q000378:metabolism*; D018920:Coculture Techniques; D005285:Fermentation; D045302:Geobacter / Q000378:metabolism*; D006859:Hydrogen / Q000032:analysis / Q000378:metabolism*; D007218:Industrial Microbiology; D056265:Microbial Interactions; D008954:Models, Biological; D013451:Sulfonic Acids / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
chemical_list: C479500:9,10-anthrahydroquinone; D000085:Acetates; D000873:Anthracenes; D056804:Biofuels; D013451:Sulfonic Acids; D006859:Hydrogen; D014994:Xylose