title: Immobilization of a thermostable cellobiose 2-epimerase from Rhodothermus marinus JCM9785 and continuous production of epilactose.
abstract: Cellobiose 2-epimerase (CE) efficiently forms epilactose which has several beneficial biological functions. A thermostable CE from Rhodothermus marinus was immobilized on Duolite A568 and packed into a column. Lactose (100 g/L) was supplied to the reactor, kept at 50 °C at a space velocity of 8 h(-1). The epilactose concentration of the resulting eluate was 30 g/L, and this was maintained for 13 d.
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, N-9, W-9, Sapporo 060-8589, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sato
authors.forename: Wataru
authors.initials: W
authors.lastname: Saburi
authors.forename: Teruyo
authors.initials: T
authors.lastname: Ojima
authors.forename: Hidenori
authors.initials: H
authors.lastname: Taguchi
authors.forename: Haruhide
authors.initials: H
authors.lastname: Mori
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsui
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D055162:Biocatalysis; D019149:Bioreactors; D002475:Cellobiose / Q000737:chemistry; D004187:Disaccharides / Q000138:chemical synthesis*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry* / Q000235:genetics; D007700:Kinetics; D007785:Lactose / Q000737:chemistry; D019998:Racemases and Epimerases / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D045245:Rhodothermus / Q000737:chemistry / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D004187:Disaccharides; D055767:Immobilized Proteins; D011994:Recombinant Proteins; D002475:Cellobiose; C047464:epilactose; D019998:Racemases and Epimerases; D007785:Lactose