title: Moniliella carnis sp. nov. and Moniliella dehoogii sp. nov., two novel species of black yeasts isolated from meat processing environments.
abstract: Thirteen strains of yeasts typical of the genus Moniliella were isolated from fermenting meat and meat processing tools in Vietnam. PCR fingerprints generated by primer (GAC)(5) subdivided the strains into two distinctive genetic groups. In a phylogenetic tree based on D1/D2 large subunit rRNA gene sequences, the strains formed a well-supported clade with Moniliella spathulata and Moniliella suaveolens but represented two new lineages. The names Moniliella carnis sp. nov. and Moniliella dehoogii sp. nov. are proposed. The two novel species can be distinguished from each other and from known species of Moniliella based on phenotypic characteristics. It is assumed that the yeasts were associated with fatty substances that contaminated the meat processing tools. The type strain of Moniliella carnis is KFP 246(T) ( = CBS 126447(T) = NRRL Y-48681(T)) and the type strain of Moniliella dehoogii is KFP 211(T) ( = CBS 126564(T) = NRRL Y-48682(T)).
authors.affiliation: Department of Microbiology, Food Industries Research Institute, 301-Nguyen Trai, Thanh Xuan, Hanoi, Vietnam.
authors.forename: Vu Nguyen
authors.initials: VN
authors.lastname: Thanh
authors.affiliation: Department of Microbiology, Food Industries Research Institute, 301-Nguyen Trai, Thanh Xuan, Hanoi, Vietnam.
authors.forename: Dao Anh
authors.initials: DA
authors.lastname: Hai
authors.affiliation: Department of Microbiology, Food Industries Research Institute, 301-Nguyen Trai, Thanh Xuan, Hanoi, Vietnam.
authors.forename: Dinh Duc
authors.initials: DD
authors.lastname: Hien
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Takashima
authors.affiliation: Department of Biology, University of Western Ontario, London, ON N6A 5B7, Canada.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Lachance
mesh_terms: D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics; D005525:Food-Processing Industry; D008460:Meat / Q000382:microbiology*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014744:Vietnam
chemical_list: D004271:DNA, Fungal; D012335:RNA, Ribosomal