title: In vitro reproduction of endochondral ossification using a 3D mesenchymal stem cell construct.
abstract: Endochondral ossification is one of the essential bone development processes in vertebrates. Although researchers from a variety of fields, including cellular/molecular biology, chemistry, and materials science, have worked to gain a better understanding of the tissue development, integration of findings from these different fields remains a major challenge. An in vitro model system that reproduces endochondral ossification would be a valuable tool for overcoming this problem, because an in vitro standardized model system can be easily accessed by researchers from different fields. Here, we fabricated a large 3D mesenchymal stem cell (MSC) construct with a ball-like morphology, which is termed a cell ball, and cultured it under a hypoxia condition, since hypoxia causes chondrogenic differentiation of MSCs in primordial cartilage, which is crucial for endochondral ossification. Region-specific chondrogenic differentiation of MSCs and mineralization within the cartilage tissue were observed in the cell ball. The precipitated minerals were detected as hydroxyapatite. Consequently, a 3D construct consisting of mineralized tissue surrounded by cartilage tissue was obtained. Moreover, the angiogenic activity of this synthesized tissue changed depending on the chondrogenic phenotype remains in the tissue, which is similar to what happens in the ossification process. Thus, this MSC cell ball system clearly reproduced the initial stage of endochondral ossification in vitro. This system is a promising tool for use as an in vitro model for investigating bone tissue development.
authors.affiliation: Department of Biomaterials Science, Osaka University, Suita, 565-0871, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Sasaki
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Egusa
authors.forename: Michiya
authors.initials: M
authors.lastname: Matsusaki
authors.forename: Akihiro
authors.initials: A
authors.lastname: Nishiguchi
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Nakano
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Akashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imazato
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yatani
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D020219:Chondrogenesis; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D000860:Hypoxia; D021621:Imaging, Three-Dimensional / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D018919:Neovascularization, Physiologic; D010012:Osteogenesis; D013696:Temperature; D013997:Time Factors
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate