title: Applying a real-time PCR assay for Histoplasma capsulatum to clinically relevant formalin-fixed paraffin-embedded human tissue.
abstract: A real-time PCR assay to detect Histoplasma capsulatum in formalin-fixed, paraffin-embedded (FFPE) tissue is described. The assay had an analytical sensitivity of 6 pg/μl of fungal DNA, analytical specificity of 100%, and clinical sensitivity of 88.9%. This proof-of-concept study may aid in the diagnosis of histoplasmosis from FFPE tissue.
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Koepsell
authors.forename: Steven H
authors.initials: SH
authors.lastname: Hinrichs
authors.forename: Peter C
authors.initials: PC
authors.lastname: Iwen
mesh_terms: D005557:Formaldehyde / Q000378:metabolism; D006658:Histoplasma / Q000235:genetics / Q000302:isolation & purification*; D006660:Histoplasmosis / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D006801:Humans; D009172:Mycology / Q000379:methods*; D010232:Paraffin / Q000378:metabolism; D057089:Pathology, Molecular / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D016610:Tissue Embedding / Q000379:methods*; D016707:Tissue Fixation / Q000379:methods*
chemical_list: D005557:Formaldehyde; D010232:Paraffin