title: Insights into fluorometabolite biosynthesis in Streptomyces cattleya DSM46488 through genome sequence and knockout mutants.
abstract: Streptomyces cattleya DSM 46488 is unusual in its ability to biosynthesise fluorine containing natural products, where it can produce fluoroacetate and 4-fluorothreonine. The individual enzymes involved in fluorometabolite biosynthesis have already been demonstrated in in vitro investigations. Candidate genes for the individual biosynthetic steps were located from recent genome sequences. In vivo inactivation of individual genes including those encoding the S-adenosyl-l-methionine:fluoride adenosyltransferase (fluorinase, SCATT_41540), 5'-fluoro-5'-deoxyadenosine phosphorylase (SCATT_41550), fluoroacetyl-CoA thioesterase (SCATT_41470), 5-fluoro-5-deoxyribose-1-phosphate isomerase (SCATT_20080) and a 4-fluorothreonine acetaldehyde transaldolase (SCATT_p11780) confirm that they are essential for fluorometabolite production. Notably gene disruption of the transaldolase (SCATT_p11780) resulted in a mutant which could produce fluoroacetate but was blocked in its ability to biosynthesise 4-fluorothreonine, revealing a branchpoint role for the PLP-transaldolase.
authors.affiliation: School of Chemistry and Centre for Biomolecular Sciences, University of St. Andrews, North Haugh, St. Andrews, Fife KY16 9ST, UK.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Zhao
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Ou
authors.forename: Ryan P
authors.initials: RP
authors.lastname: McGlinchey
authors.forename: David
authors.initials: D
authors.lastname: O'Hagan
mesh_terms: D005463:Fluoroacetates / Q000378:metabolism*; D055786:Gene Knockout Techniques; D005798:Genes, Bacterial; D005810:Multigene Family; D009154:Mutation; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D013912:Threonine / Q000031:analogs & derivatives* / Q000235:genetics / Q000378:metabolism; D014153:Transaldolase / Q000235:genetics / Q000378:metabolism
chemical_list: D005463:Fluoroacetates; C048271:4-fluorothreonine; D013912:Threonine; C014815:fluoroacetic acid; D014153:Transaldolase