title: Dynamics of structural transformations of BCG granulomas and expression of TNF-α and granulocyte-macrophage CSF by macrophages in vitro.
abstract: The spleens were isolated from mice at different times after BCG infection and BCG granulomas were explanted and cultured in vitro. Cell migration, chemoattractant potential, and expression of TNF-α and granulocyte-macrophage CSF (GM-CSF) by macrophages migrated from granulomas were evaluated in granulomas. The number of macrophages able to migrate; migrating out of granulomas, expressing TNF-α and GM-CSF decreased with increasing the time after infection. The number of cells in "dissociating" granulomas correlates with the number of macrophages containing live BCG mycobacteria in the cytoplasm.
authors.affiliation: Research Center of Clinical and Experimental Medicine, Siberian Division of the Russian Academy of Medical Sciences, Novosibirsk, Russia. arkhipov@soramn.ru
authors.forename: S A
authors.initials: SA
authors.lastname: Arkhipov
authors.forename: V A
authors.initials: VA
authors.lastname: Shkurupy
authors.forename: E S
authors.initials: ES
authors.lastname: Akhramenko
authors.forename: D A
authors.initials: DA
authors.lastname: Iljine
authors.forename: M V
authors.initials: MV
authors.lastname: Zaikovskaja
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000009:adverse effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D006099:Granuloma / Q000209:etiology* / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D001500:BCG Vaccine; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor