title: [The effects of recombinant porcine amelogenin on human gingival epithelial cells].
abstract: OBJECTIVE
  To investigate the potential effect of recombinant 25kDa porcine amelogenin (rPAm) on attachment, proliferation and migration of primarily cultured human gingival epithelial cells (HGEC).
  
  
  METHODS
  The second passage of HGECs were exposed to different concentrations of rPAm (0, 5, 10, 20μg/mL, respectively). Proliferation and attachment activities was measured by using cell counting method. Cellular migration was assayed by using an in vitro wound healing model. The data was quantified by the analysis of GraphPad Prism software.
  
  
  RESULTS
  rPAm inhibited HGEC attachment in the adhesion assay, the effect was depended on time and rPAm dose. rPAm suppressed the growth rate of HGEC, that was also dose and time dependent. rPAm inhibited the migration ability of HGEC, the concentration of 20μg/mL group had the most significant effect.
  
  
  CONCLUSIONS
  rPAm significantly inhibit the growth rate, cell adhesion and migration of HGEC, and the effect was dose- and time- dependent.
authors.affiliation: Department of Periodontology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou 510055, Guangdong Province, China. lixiting2010@gmail.com
authors.forename: Xi-ting
authors.initials: XT
authors.lastname: Li
authors.forename: Rong
authors.initials: R
authors.lastname: Shu
authors.forename: Zhong-chen
authors.initials: ZC
authors.lastname: Song
authors.forename: Yan-bin
authors.initials: YB
authors.lastname: Zhou
mesh_terms: D053523:Amelogenin; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D002478:Cells, Cultured; D004847:Epithelial Cells; D005881:Gingiva; D006801:Humans; D013552:Swine; D014945:Wound Healing
chemical_list: D053523:Amelogenin