title: Amphibacillus marinus sp. nov., a member of the genus Amphibacillus isolated from marine mud.
abstract: A gram-positive, spore-forming, rod-shaped bacterium, designated J1(T) was isolated from deep-sea mud collected from the South China Sea and subjected to polyphasic taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequences revealed that J1(T) clustered with the type strains of Amphibacillus cookii, Amphibacillus sediminis and Amphibacillus jilinensis and exhibited a range of similarity of 93.9-97.0 % to members of the genus Amphibacillus. The DNA G+C content was 36.7 mol%. Chemotaxonomic analysis showed no quinones, and the cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid for strain J1(T). The major cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. Strain J1(T) was positive for catalase activity and negative for oxidase activity. On the basis of phylogenetic position and phenotypic properties, strain J1(T) represents a novel species of the genus Amphibacillus and the name Amphibacillus marinus sp. nov. is proposed. The type strain is J1(T) ( = CGMCC 1.10434(T) = JCM 17099(T)).
authors.affiliation: Graduate School of Chinese Academy of Sciences, Beijing, 100049, PR China.|Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Biao
authors.initials: B
authors.lastname: Ren
authors.affiliation: Graduate School of Chinese Academy of Sciences, Beijing, 100049, PR China.|Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Ma
authors.affiliation: Graduate School of Chinese Academy of Sciences, Beijing, 100049, PR China.|Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Graduate School of Chinese Academy of Sciences, Beijing, 100049, PR China.|Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xie
authors.affiliation: Graduate School of Chinese Academy of Sciences, Beijing, 100049, PR China.|Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Zhi-Heng
authors.initials: ZH
authors.lastname: Liu
authors.affiliation: Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
authors.forename: Benoît
authors.initials: B
authors.lastname: Pugin
authors.affiliation: South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China.|Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, PR China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Zhang
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002374:Catalase / Q000378:metabolism; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid; D002374:Catalase