title: Expression of (pro)renin receptor in human erythroid cell lines and its increased protein accumulation by interferon-γ.
abstract: The renin-angiotensin system is known to enhance erythropoiesis. (Pro)renin receptor ((P)RR), a specific receptor for renin and prorenin, has recently been identified. However, expression of (P)RR in erythroid cells has not been studied. The aim of the present study is to clarify expression of (P)RR in erythroid cells, and the effects of erythropoietin, angiotensin II, transforming growth factor-β1 (TGF-β1), interferon-γ (IFN-γ) and interleukin-1β (IL-1β) on its expression. Western blot analysis showed that (P)RR protein was expressed in human cultured erythroid cell lines, YN-1 and YN-1-0-A (a clonal variant cell line of YN-1). Erythropoietin (1IU/ml) increased (P)RR mRNA expression levels in YN-1-0-A cells (1.7-fold increase compared with control), but angiotensin II did not. Treatment of YN-1-0-A cells with IFN-γ (10ng/ml) for 48h increased the expression levels of (P)RR protein significantly (1.4-fold increase compared with control), whereas it had no significant effects on expression levels of (P)RR mRNA. Treatment of YN-1-0-A cells with TGF-β1 or IL-1β for 24 or 48h had no significant effects on expression levels of (P)RR. The present study has shown for the first time expression of (P)RR in erythroid cells, raising the possibility that (P)RR may have a role in erythropoiesis and the pathophysiology of certain types of anemia.
authors.affiliation: Department of Endocrinology and Applied Medical Science, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-8575, Japan.
authors.forename: Kiriko
authors.initials: K
authors.lastname: Kaneko
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nishiyama
authors.forename: Koji
authors.initials: K
authors.lastname: Ohba
authors.forename: Akiko
authors.initials: A
authors.lastname: Shibasaki
authors.forename: Takuo
authors.initials: T
authors.lastname: Hirose
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Totsune
authors.forename: Kazumichi
authors.initials: K
authors.lastname: Furuyama
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Takahashi
mesh_terms: D002478:Cells, Cultured; D041905:Erythroid Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004920:Erythropoiesis; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D000093342:Prorenin Receptor
chemical_list: D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D007371:Interferon-gamma; D000093342:Prorenin Receptor