title: Dopamine and serotonin modulate human GABAρ1 receptors expressed in Xenopus laevis oocytes.
abstract: GABAρ1 receptors are highly expressed in bipolar neurons of the retina and to a lesser extent in several areas of the central nervous system (CNS), and dopamine and serotonin are also involved in the modulation of retinal neural transmission. Whether these biogenic amines have a direct effect on ionotropic GABA receptors was not known. Here, we report that GABAρ1 receptors, expressed in X. laevis oocytes, were negatively modulated by dopamine and serotonin and less so by octopamine and tyramine. Interestingly, these molecules did not have effects on GABA(A) receptors. 5-Carboxamido-tryptamine and apomorphine did not exert evident effects on any of the receptors. Schild plot analyses of the inhibitory actions of dopamine and serotonin on currents elicited by GABA showed slopes of 2.7 ± 0.3 and 6.1 ± 1.8, respectively, indicating a noncompetitive mechanism of inhibition. The inhibition of GABAρ1 currents was independent of the membrane potential and was insensitive to picrotoxin, a GABA receptor channel blocker and to the GABAρ-specific antagonist (1,2,5,6-tetrahydropyridine-4-yl)methyl phosphinic acid (TPMPA). Dopamine and serotonin changed the sensitivity of GABAρ1 receptors to the inhibitory actions of Zn(2+). In contrast, La(3+) potentiated the amplitude of the GABA currents generated during negative modulation by dopamine (EC(50) 146 μM) and serotonin (EC(50) 196 μM). The functional role of the direct modulation of GABAρ receptors by dopamine and serotonin remains to be elucidated; however, it may represent an important modulatory pathway in the retina, where GABAρ receptors are highly expressed and where these biogenic amines are abundant.
authors.affiliation: Departamento de Neurobiología Celular y Molecular, Laboratorio de Neurobiología Molecular y Celular I-II, Instituto de Neurobiología, Campus UNAM Juriquilla, Querétaro, Qro. México CP 76230.
authors.forename: Lenin D
authors.initials: LD
authors.lastname: Ochoa-de la Paz
authors.forename: Argel
authors.initials: A
authors.lastname: Estrada-Mondragón
authors.forename: Agenor
authors.initials: A
authors.lastname: Limón
authors.forename: Ricardo
authors.initials: R
authors.lastname: Miledi
authors.forename: Ataúlfo
authors.initials: A
authors.lastname: Martínez-Torres
mesh_terms: D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D003627:Data Interpretation, Statistical; D004298:Dopamine / Q000502:physiology*; D018756:GABA Antagonists / Q000494:pharmacology; D006801:Humans; D008564:Membrane Potentials / Q000187:drug effects; D009655:Octopamine / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D010721:Phosphinic Acids / Q000494:pharmacology; D010852:Picrotoxin / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011963:Receptors, GABA-A / Q000187:drug effects; D018080:Receptors, GABA-B / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D012701:Serotonin / Q000502:physiology*; D014439:Tyramine / Q000494:pharmacology; D014982:Xenopus laevis
chemical_list: D004298:Dopamine; D018756:GABA Antagonists; D009655:Octopamine; D010721:Phosphinic Acids; D010852:Picrotoxin; D011725:Pyridines; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D012701:Serotonin; D014439:Tyramine; C101879:(1,2,5,6-tetrahydropyridin-4-yl)methylphosphinic acid