title: Interactome-wide analysis identifies end-binding protein 1 as a crucial component for the speck-like particle formation of activated absence in melanoma 2 (AIM2) inflammasomes.
abstract: Inflammasomes are cytoplasmic receptors that can recognize intracellular pathogens or danger signals and are critical for interleukin 1β production. Although several key components of inflammasome activation have been identified, there has not been a systematic analysis of the protein components found in the stimulated complex. In this study, we used the isobaric tags for relative and absolute quantification approach to systemically analyze the interactomes of the NLRP3, AIM2, and RIG-I inflammasomes in nasopharyngeal carcinoma cells treated with specific stimuli of these interactomes (H2O2, poly (dA:dT), and EBV noncoding RNA, respectively). We identified a number of proteins that appeared to be involved in the interactomes and also could be precipitated with anti-apoptosis-associated speck-like protein containing caspase activation and recruitment domain antibodies after stimulation. Among them, end binding protein 1 was an interacting component in all three interactomes. Silencing of end binding protein 1 expression by small interfering RNA inhibited the activation of the three inflammasomes, as indicated by reduced levels of interleukin 1β secretion. We confirmed that end binding protein 1 directly interacted with AIM2 and ASC in vitro and in vivo. Most importantly, fluorescence confocal microscopy showed that end binding protein 1 was required for formation of the speck-like particles that represent activation of the AIM2 inflammasome. In nasopharyngeal carcinoma tissues, immunohistochemical staining showed that end binding protein 1 expression was elevated and significantly correlated with AIM2 and ASC expression in nasopharyngeal carcinoma tumor cells. In sum, we profiled the interactome components of three inflammasomes and show for the first time that end binding protein 1 is crucial for the speck-like particle formation that represents activated inflammasomes.
authors.affiliation: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung Molecular Medicine Research Center, Department of Medical Biotechnology and Laboratory Science, Chang Gung University, and Department of Radiation Oncology, Chang Gung Memorial Hospital at Lin-Kou, Tao-Yuan, Taiwan.
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Wang
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Hsu
authors.forename: Chiu-Chin
authors.initials: CC
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.forename: Lih-Chyang
authors.initials: LC
authors.lastname: Chen
authors.forename: David M
authors.initials: DM
authors.lastname: Ojcius
authors.forename: Ngan-Ming
authors.initials: NM
authors.lastname: Tsang
authors.forename: Chuen
authors.initials: C
authors.lastname: Hsueh
authors.forename: Chih-Ching
authors.initials: CC
authors.lastname: Wu
authors.forename: Yu-Sun
authors.initials: YS
authors.lastname: Chang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D053468:CARD Signaling Adaptor Proteins; D002277:Carcinoma; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D058847:Inflammasomes / Q000378:metabolism*; D007553:Isotope Labeling; D008297:Male; D013058:Mass Spectrometry; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology; D009687:Nuclear Proteins / Q000378:metabolism*; D011067:Poly dA-dT / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D055815:Young Adult
chemical_list: C410167:AIM2 protein, human; D053468:CARD Signaling Adaptor Proteins; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D058847:Inflammasomes; C500985:MAPRE1 protein, human; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C402202:PYCARD protein, human; D011067:Poly dA-dT