title: [Chemical constituents contained in Populus tomentosa].
abstract: OBJECTIVE
  To separate and identify chemical constituents from stem barks of male plants of Populus tomentosa.
  
  
  METHODS
  Fresh stem barks of P. tomentosa were extracted with methanol to obtain extracts which were suspended in water and blended successively with petroleum ether, ethyl acetate and n-butanol. Various chromatographic techniques were used to separate and purify the constituents extracted with ethyl acetate and n-butanol fractions. Their structures were identified on the basis of their physicochemical properties and spectral data.
  
  
  RESULTS
  Twelve compounds were separated with ethyl acetate and n-butanol fractions and identified as benzoic acid (1), daucosterol (2), tremuloidin (3), rhamnocitrin (4), sakuranetin (5), 7-O-methylaromadendrin (6), isograndidentatin A (7), siebolside B (8), sakuranin (9), micranthoside (10), alpha-D-glucopyranose (11), and sucrose (12).
  
  
  CONCLUSIONS
  Compounds 4-12 were separated from this plant for the first time. Of them, compound 10 was separated from this plant genus for the first time.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. liuhaiping0@yahoo.com.cn
authors.forename: Haiping
authors.initials: H
authors.lastname: Liu
authors.forename: Zhimao
authors.initials: Z
authors.lastname: Chao
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wu
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Tan
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Sun
mesh_terms: D005419:Flavonoids / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D010636:Phenols / Q000032:analysis; D032107:Populus / Q000737:chemistry*; D000094162:Phytoalexins
chemical_list: D005419:Flavonoids; D005960:Glucosides; D010636:Phenols; C513727:siebolside B; C099724:sakuranetin; D000094162:Phytoalexins