title: Identification and sequence analysis of grain softness protein in selected wheat, rye and triticale.
abstract: Grain  softness protein (GSP) is an important protein for overcoming milling and grain  defenses in the innate immunity systems of cereals. The objective of this study  was to evaluate and understand GSP sequences in selected wheat, rye and  triticale. Using sequences for this gene from a sequence database, we performed  clustering analysis to compare the sequences obtained from 3 germplasms with  other studied sequences for GSP. The maximum difference between the Hirmand GSP  genotype in wheat and the database sequences was 23% in EF109396 and EF109399.  Most amino acid variation between the GSP sequences involved the same amino  acids. The Nikita rye GSP gene showed 64% identity with DQ269918 and  AY667063. The isoelectric point in the GSP of wheat and Lasko triticale was  significantly higher than that of rye GSP. In addition, parameters such as  optical density, grand average of hydrophobicity, percentage of hydrophobicity  and hydrophilic amino acids, and number of alpha helices and beta sheets in GSP  were similar in wheat and triticale but not in wheat and rye.
authors.affiliation: Department of Genetics, Agriculture University of Tajikistan, Dushanbe, Tajikistan. shirazi60@yahoo.com
authors.forename: M A S
authors.initials: MA
authors.lastname: Kharrazi
authors.forename: V
authors.initials: V
authors.lastname: Bobojonov
mesh_terms: D001483:Base Sequence; D002523:Edible Grain / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry*; D012434:Secale / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein / Q000379:methods*; D014908:Triticum / Q000378:metabolism*
chemical_list: C088852:GSP-1 protein, Triticum aestivum; D010940:Plant Proteins