title: Biochemical activities of acetone extracts of Hyssopus angustifolius.
abstract: Antioxidant and antihemolytic activities of acetone extracts of Hyssopus angustifolius flowers, leaf and stems were investigated employing different in vitro and ex vivo assay systems. IC50, for 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical-scavenging activity were 239.4 +/- 8.4 microg/mL for flowers, 357.8 +/- 11.1 microg/mL for stems and 182.5 +/- 7.5 microg/mL for leaf. All extracts showed moderate nitric oxide scavenging activity. The leaf extract exhibited better hydrogen peroxide scavenging and Fe2+ chelating activity than the others (IC50 were 261.0 +/- 6.2 microg/mL for hydrogen peroxide and 534.0 +/- 9.9 microg/mL for Fe3+ chelating activity). The extracts exhibited good antioxidant activity in linoleic acid peroxidation system and weak reducing power ability. The leaf extract showed better antihemolytic activity than the flower and stem (IC50 = 65.7 +/- 1.8 microg/mL).
authors.affiliation: Department of Organic Chemistry, Faculty of Chemistry, University of Mazandran, 47415, Babolsar, Iran. Nabavi208@gmail.com
authors.forename: Heshmatollah
authors.initials: H
authors.lastname: Alinezhad
authors.forename: Robabeh
authors.initials: R
authors.lastname: Baharfar
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Zare
authors.forename: Razieh
authors.initials: R
authors.lastname: Azimi
authors.forename: Seyed Fazel
authors.initials: SF
authors.lastname: Nabavi
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Nabavi
mesh_terms: D000096:Acetone / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D035264:Flowers; D006461:Hemolysis / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000737:chemistry; D007502:Iron Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019686:Lamiaceae / Q000737:chemistry; D019787:Linoleic Acid / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009569:Nitric Oxide / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D018547:Plant Stems; D051381:Rats; D012997:Solvents / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D007502:Iron Chelating Agents; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D000096:Acetone; D009569:Nitric Oxide; D019787:Linoleic Acid; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl