title: Association of the miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G polymorphisms with risk of spontaneously aborted fetuses.
abstract: OBJECTIVE
  The miR-196a2T>C and miR-499A>G polymorphisms have been reported to be genetic risk factors for recurrent spontaneous abortion; however, that previous study focused on the genetic analyses of pregnant women rather than aborted fetuses. Because annexin A1 is a target of miR-196a2 and is related to anti-inflammation, miR-196a2 may be immunologically important. Moreover, miR-146a, miR-149, miR-196a2, and miR-499 have shown associations with immune responses.
  
  
  METHODS
  One hundred and eighty-two spontaneously aborted fetuses (SAFs) were <20 weeks of gestational age. The control subjects were 101 healthy children and 302 adults collected from a convenience sample. Polymerase chain reaction and restriction fragment length polymorphism analysis was performed to identify the miR-146aC>G, miR-149T>C, miR-196a2T>C, and miR-499A>G genotypes.
  
  
  RESULTS
  Chromosomally normal SAFs had significantly different frequencies of the miR-196a2CC, miR-146aCC/miR-196a2CC, and miR-149TT/miR-196a2CC genotypes compared with control subjects.
  
  
  CONCLUSIONS
  miR-196a2CC, miR-146aCC/miR-196a2CC, and miR-149TT/miR-196a2CC in fetuses are possible risk factors for spontaneous abortion.
authors.affiliation: Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, Korea.
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Su Yeoun
authors.initials: SY
authors.lastname: Kim
authors.forename: HyungChul
authors.initials: H
authors.lastname: Rah
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Choi
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Cha
authors.forename: Tae Ki
authors.initials: TK
authors.lastname: Yoon
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Lee
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Shim
authors.forename: Nam Keun
authors.initials: NK
authors.lastname: Kim
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D005865:Gestational Age; D006801:Humans; D007723:Korea; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011247:Pregnancy; D012307:Risk Factors
chemical_list: C542542:MIRN146 microRNA, human; C551557:MIRN149 microRNA, human; C542543:MIRN196 microRNA, human; C548683:MIRN499 microRNA, human; D035683:MicroRNAs