title: Atmospheric scanning electron microscope for correlative microscopy.
abstract: The JEOL ClairScope is the first truly correlative scanning electron and optical microscope. An inverted scanning electron microscope (SEM) column allows electron images of wet samples to be obtained in ambient conditions in a biological culture dish, via a silicon nitride film window in the base. A standard inverted optical microscope positioned above the dish holder can be used to take reflected light and epifluorescence images of the same sample, under atmospheric conditions that permit biochemical modifications. For SEM, the open dish allows successive staining operations to be performed without moving the holder. The standard optical color camera used for fluorescence imaging can be exchanged for a high-sensitivity monochrome camera to detect low-intensity fluorescence signals, and also cathodoluminescence emission from nanophosphor particles. If these particles are applied to the sample at a suitable density, they can greatly assist the task of perfecting the correlation between the optical and electron images.
authors.affiliation: Technology Facility, Biology Department, University of York, York YO10 5DD, UK.
authors.forename: Ian E G
authors.initials: IE
authors.lastname: Morrison
authors.forename: Clare L
authors.initials: CL
authors.lastname: Dennison
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Nishiyama
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Suga
authors.forename: Chikara
authors.initials: C
authors.lastname: Sato
authors.forename: Andrew
authors.initials: A
authors.lastname: Yarwood
authors.forename: Peter J
authors.initials: PJ
authors.lastname: O'Toole
mesh_terms: D000818:Animals; D019556:COS Cells; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D002875:Chromosomes / Q000648:ultrastructure; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D021962:Membrane Microdomains / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009174:Mycoplasma / Q000648:ultrastructure; D011956:Receptors, Cell Surface / Q000648:ultrastructure; D011994:Recombinant Proteins / Q000096:biosynthesis; D016707:Tissue Fixation
chemical_list: D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins