title: Skin prick test reactivity to aeroallergens by filaggrin mutation status.
abstract: BACKGROUND
  Studies have shown that filaggrin gene (FLG) mutations are positively associated with sensitization to aero allergens. We hypothesized that FLG mutations would also have an effect on the mean size of positive skin prick test (SPT) reactions as well as the number of positive reactions.
  
  
  OBJECTIVE
  To investigate the effect of FLG mutations on the mean size and the number of positive SPT reactions, as well as the association with positive specific IgE.
  
  
  METHODS
  A random sample of 3335 adults from the general population in Denmark was genotyped for the R501X and 2282del4 mutations in the FLG. SPT and specific IgE measurements to common aeroallergens were also performed.
  
  
  RESULTS
  FLG mutations did not influence the mean size and number of positive SPT reactions. Also, no association was found between FLG mutations and specific IgE measurements.
  
  
  CONCLUSIONS
  Our findings suggest that FLG mutations alone are insufficient to cause secondary sensitization to allergens. The positive association seen in patients must be explained by a combination of further barrier abnormality caused by dermatitis as well as increased allergen exposure.
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, DenmarkResearch Centre for Prevention and Health, Glostrup Hospital, Copenhagen University Hospital Glostrup, Glostrup, DenmarkDepartment of Clinical Biochemistry, Copenhagen University Hospital Gentofte, Hellerup, DenmarkDepartment of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark.
authors.forename: M G
authors.initials: MG
authors.lastname: Hougaard
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, DenmarkResearch Centre for Prevention and Health, Glostrup Hospital, Copenhagen University Hospital Glostrup, Glostrup, DenmarkDepartment of Clinical Biochemistry, Copenhagen University Hospital Gentofte, Hellerup, DenmarkDepartment of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark.
authors.forename: J D
authors.initials: JD
authors.lastname: Johansen
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, DenmarkResearch Centre for Prevention and Health, Glostrup Hospital, Copenhagen University Hospital Glostrup, Glostrup, DenmarkDepartment of Clinical Biochemistry, Copenhagen University Hospital Gentofte, Hellerup, DenmarkDepartment of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Linneberg
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, DenmarkResearch Centre for Prevention and Health, Glostrup Hospital, Copenhagen University Hospital Glostrup, Glostrup, DenmarkDepartment of Clinical Biochemistry, Copenhagen University Hospital Gentofte, Hellerup, DenmarkDepartment of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Bandier
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, DenmarkResearch Centre for Prevention and Health, Glostrup Hospital, Copenhagen University Hospital Glostrup, Glostrup, DenmarkDepartment of Clinical Biochemistry, Copenhagen University Hospital Gentofte, Hellerup, DenmarkDepartment of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Stender
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, DenmarkResearch Centre for Prevention and Health, Glostrup Hospital, Copenhagen University Hospital Glostrup, Glostrup, DenmarkDepartment of Clinical Biochemistry, Copenhagen University Hospital Gentofte, Hellerup, DenmarkDepartment of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark.
authors.forename: B C
authors.initials: BC
authors.lastname: Carlsen
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, DenmarkResearch Centre for Prevention and Health, Glostrup Hospital, Copenhagen University Hospital Glostrup, Glostrup, DenmarkDepartment of Clinical Biochemistry, Copenhagen University Hospital Gentofte, Hellerup, DenmarkDepartment of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark.
authors.forename: P B
authors.initials: PB
authors.lastname: Szecsi
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, DenmarkResearch Centre for Prevention and Health, Glostrup Hospital, Copenhagen University Hospital Glostrup, Glostrup, DenmarkDepartment of Clinical Biochemistry, Copenhagen University Hospital Gentofte, Hellerup, DenmarkDepartment of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Meldgaard
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, DenmarkResearch Centre for Prevention and Health, Glostrup Hospital, Copenhagen University Hospital Glostrup, Glostrup, DenmarkDepartment of Clinical Biochemistry, Copenhagen University Hospital Gentofte, Hellerup, DenmarkDepartment of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Menné
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, DenmarkResearch Centre for Prevention and Health, Glostrup Hospital, Copenhagen University Hospital Glostrup, Glostrup, DenmarkDepartment of Clinical Biochemistry, Copenhagen University Hospital Gentofte, Hellerup, DenmarkDepartment of Dermato-Allergology, Copenhagen University Hospital Gentofte, Hellerup, Denmark.
authors.forename: J P
authors.initials: JP
authors.lastname: Thyssen
mesh_terms: D000485:Allergens / Q000276:immunology*; D005260:Female; D000091344:Filaggrin Proteins; D006579:Heterozygote; D006801:Humans; D006967:Hypersensitivity / Q000235:genetics* / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood*; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D012882:Skin Tests
chemical_list: D000485:Allergens; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D007073:Immunoglobulin E