title: Status of lipid peroxidation and antioxidant enzymes in goats naturally infected with Babesia ovis.
abstract: This study aimed to assess lipid peroxidation and antioxidant enzymes in goats naturally infected with Babesia ovis. Red blood cell count (RBC), hemoglobin (Hb) concentration, packed cell volume (PCV), malondialdehyde (MDA) concentration, erythrocyte superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities and total antioxidant capacity (TAC) were determined in 15 goats naturally infected with B. ovis as well as same number of healthy goats. The parasitological diagnosis was confirmed using polymerase chain reaction (PCR) analysis by amplifying a partial 18S rRNA gene sequence of B. ovis. Percentage of parasitemia varied from 0.01 to 1%. The activities of erythrocyte GSH-Px, SOD, CAT and TAC were significantly lower (p<0.05) in the infected goats than in healthy ones. MDA concentration in erythrocytes of infected goats was significantly higher in infected goats than in healthy ones (p<0.05). Severity of parasitemia showed a positive correlation with the MDA and negative correlation with PCV, SOD, CAT, GSH-Px and TAC. Also, MDA was negatively correlated with PCV, SOD, CAT, GSH-Px and TAC. The results of this study suggested that oxidative damage to RBCs may contribute to the pathogenesis of anemia in caprine babesiosis.
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Iran.
authors.forename: Bijan
authors.initials: B
authors.lastname: Esmaeilnejad
authors.forename: Mousa
authors.initials: M
authors.lastname: Tavassoli
authors.forename: Siamak
authors.initials: S
authors.lastname: Asri-Rezaei
authors.forename: Bahram
authors.initials: B
authors.lastname: Dalir-Naghadeh
authors.forename: Hassan
authors.initials: H
authors.lastname: Malekinejad
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001403:Babesia / Q000145:classification*; D001404:Babesiosis / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D004912:Erythrocytes / Q000201:enzymology; D015511:Goat Diseases / Q000378:metabolism / Q000469:parasitology*; D006041:Goats; D015227:Lipid Peroxidation / Q000502:physiology*; D018512:Parasitemia
chemical_list: D000975:Antioxidants