title: Effects of unconjugated bilirubin on chromosomal damage in individuals with Gilbert's syndrome measured with the micronucleus cytome assay.
abstract: Circulating unconjugated bilirubin (UCB) has been reported to protect against lung and colorectal cancer. The present study aimed to explore, for the first time, whether mildly elevated circulating UCB, as found in Gilbert`s syndrome (GS), is associated with changes of DNA damage. A random 76 individuals, matched for age and gender, were recruited from the general population and allocated into the GS group (UCB ≥ 17.1 µM; n = 38) or control group (UCB <17.1 µM; n = 38). Chromosomal and cytological changes were determined in lymphocytes and buccal cells using the cytokinesis-block micronucleus cytome assay (CBMN) and buccal micronucleus cytome assay (BMcyt). No significant differences were found between GS subjects and the control group in the CBMN and BMcyt determined endpoints. Subsequently, when age dependency of effects were analysed, lower formation of buccal micronucleated cells (by 73.3%) and buccal nuclear buds (by 70.9%) in the GS subgroup ≥ 30 years were found, compared to the GS subgroup <30 years. These findings suggest DNA protection in epithelial tissue of older individuals with GS.
authors.affiliation: Department of Nutritional Sciences, Emerging Field Oxidative Stress and DNA Stability, University of Vienna, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Wallner
authors.forename: S M
authors.initials: SM
authors.lastname: Blassnigg
authors.forename: K
authors.initials: K
authors.lastname: Marisch
authors.forename: M T
authors.initials: MT
authors.lastname: Pappenheim
authors.forename: E
authors.initials: E
authors.lastname: Müllner
authors.forename: C
authors.initials: C
authors.lastname: Mölzer
authors.forename: A
authors.initials: A
authors.lastname: Nersesyan
authors.forename: R
authors.initials: R
authors.lastname: Marculescu
authors.forename: D
authors.initials: D
authors.lastname: Doberer
authors.forename: S
authors.initials: S
authors.lastname: Knasmüller
authors.forename: A C
authors.initials: AC
authors.lastname: Bulmer
authors.forename: K H
authors.initials: KH
authors.lastname: Wagner
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001663:Bilirubin / Q000009:adverse effects / Q000097:blood*; D002869:Chromosome Aberrations; D015179:Colorectal Neoplasms / Q000473:pathology; D020552:Comet Assay / Q000379:methods*; D048749:Cytokinesis; D004249:DNA Damage; D023381:Endpoint Determination; D005260:Female; D005492:Folic Acid / Q000097:blood; D005878:Gilbert Disease / Q000097:blood / Q000235:genetics*; D006710:Homocysteine / Q000097:blood; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology; D008214:Lymphocytes / Q000473:pathology; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D008875:Middle Aged; D014805:Vitamin B 12 / Q000097:blood; D055815:Young Adult
chemical_list: D006710:Homocysteine; D005492:Folic Acid; D014805:Vitamin B 12; D001663:Bilirubin