title: Expanding the genetic code of Drosophila melanogaster.
abstract: Genetic code expansion for unnatural amino acid mutagenesis has, until recently, been limited to cell culture. We demonstrate the site-specific incorporation of unnatural amino acids into proteins in Drosophila melanogaster at different developmental stages, in specific tissues and in a subset of cells within a tissue. This approach provides a foundation for probing and controlling processes in this established metazoan model organism with a new level of molecular precision.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: Ambra
authors.initials: A
authors.lastname: Bianco
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Townsley
authors.forename: Sebastian
authors.initials: S
authors.lastname: Greiss
authors.forename: Kathrin
authors.initials: K
authors.lastname: Lang
authors.forename: Jason W
authors.initials: JW
authors.lastname: Chin
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005815:Genetic Code