title: A universal indicator dye pH assay for crystallization solutions and other high-throughput applications.
abstract: In protein crystallization, as well as in many other fields, it is known that the pH at which experiments are performed is often the key factor in the success or failure of the trials. With the trend towards plate-based high-throughput experimental techniques, measuring the pH values of solutions one by one becomes prohibitively time- and reagent-expensive. As part of an HT crystallization facility, a colour-based pH assay that is rapid, uses very little reagent and is suitable for 96-well or higher density plates has been developed.
authors.affiliation: Materials Science and Engineering, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia. janet.newman@csiro.au
authors.forename: Janet
authors.initials: J
authors.lastname: Newman
authors.forename: Roger A
authors.initials: RA
authors.lastname: Sayle
authors.forename: Vincent J
authors.initials: VJ
authors.lastname: Fazio
mesh_terms: D001671:Biochemistry / Q000379:methods; D002138:Calibration; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents / Q000737:chemistry* / Q000592:standards; D003460:Crystallization / Q000592:standards; D018360:Crystallography, X-Ray / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry* / Q000592:standards; D011506:Proteins / Q000737:chemistry; D012996:Solutions; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D007202:Indicators and Reagents; D011506:Proteins; D012996:Solutions