title: A baculovirus isolated from wild silkworm encompasses the host ranges of Bombyx mori nucleopolyhedrosis virus and Autographa californica multiple nucleopolyhedrovirus in cultured cells.
abstract: A baculovirus, named BomaNPV S2, was isolated from a diseased larva of the wild silkworm, Bombyx mandarina. Notably, BomaNPV S2 exhibited a distinguishing feature in that its host range covered that of both Bombyx mori nucleopolyhedrosis virus (BmNPV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) in cultured cells. It could replicate in cells of B. mori (Bm5 and BmN), Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn-5B1-4). However, occlusion-derived virions of BomaNPV S2 in B. mori cells contained only a single nucleocapsid, whereas they contained multiple nucleocapsids in Tn-5B1-4 cells. The complete genome sequence of BomaNPV S2, including predicted ORFs, was determined and compared with the genome sequence of its close relatives. The comparison results showed that most of the BomaNPV S2 genome sequence was shared with BmNPV (BmNPV T3) or BomaNPV S1, but several regions seemed more similar to regions of AcMNPV. This observation might explain why BomaNPV S2 covers the host ranges of BmNPV and AcMNPV. Further recombinant virus infection experiments demonstrated that GP64 plays an important role in BomaNPV S2 host-range determination.
authors.affiliation: Institute of Insect Science, Zhejiang University, Hangzhou 310058, PR China.|Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, PR China.
authors.forename: Yi-Peng
authors.initials: YP
authors.lastname: Xu
authors.affiliation: Institute of Insect Science, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Lin-Zhu
authors.initials: LZ
authors.lastname: Gu
authors.affiliation: Institute of Insect Science, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Yi-Han
authors.initials: YH
authors.lastname: Lou
authors.affiliation: Institute of Insect Science, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Ruo-Lin
authors.initials: RL
authors.lastname: Cheng
authors.affiliation: Institute of Insect Science, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Xu
authors.affiliation: School of Life Science, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Wen-Bing
authors.initials: WB
authors.lastname: Wang
authors.affiliation: Institute of Insect Science, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012831:Bombyx / Q000821:virology*; D002460:Cell Line; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D016865:Reassortant Viruses; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D014764:Viral Proteins