title: Fibroblast growth factor 10 in human ovaries.
abstract: The expression of fibroblast growth factor 10 (FGF-10) has not been studied in human ovarian cortical follicles. The aim of the present study was to investigate the expression of FGF-10 in preantral follicles from fetuses, girls and women. Ovarian samples were obtained from 14 human fetuses at 21-33 gestational weeks and from 35 girls and women aged 5-39 years. The specimens were prepared for detection of the FGF-10 protein by immunohistochemistry. Reverse-transcription PCR was applied to ovarian extracts to identify FGF-10 mRNA transcripts. In fetal tissue, the FGF-10 protein was detected in oocytes in 50% of the samples and in granulosa cells in 30%. In ovarian tissue from girls and women, the FGF-10 protein was detected in oocytes and granulosa cells in all samples. FGF-10 mRNA transcripts were present in all adult and fetal samples tested. The identification of FGF-10 at both the protein and mRNA levels suggests that FGF-10 may contribute to human preantral follicle development.
authors.affiliation: Infertility and IVF Unit, Hospital for Women, Rabin Medical Center, Petach Tikva 49100, Israel.
authors.forename: Galia
authors.initials: G
authors.lastname: Oron
authors.forename: Benjamin
authors.initials: B
authors.lastname: Fisch
authors.forename: Xiao Yun
authors.initials: XY
authors.lastname: Zhang
authors.forename: Rinat
authors.initials: R
authors.lastname: Gabbay-Benziv
authors.forename: Gania
authors.initials: G
authors.lastname: Kessler-Icekson
authors.forename: Haim
authors.initials: H
authors.lastname: Krissi
authors.forename: Avi
authors.initials: A
authors.lastname: Ben-Haroush
authors.forename: Asangla
authors.initials: A
authors.lastname: Ao
authors.forename: Ronit
authors.initials: R
authors.lastname: Abir
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D051526:Fibroblast Growth Factor 10 / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D009865:Oocytes / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D006080:Ovarian Follicle / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: C496463:FGF10 protein, human; D051526:Fibroblast Growth Factor 10; D012333:RNA, Messenger