title: [Research on the preparative method of Arctigenin].
abstract: OBJECTIVE
  To research on the preparation of Arctigenin in vitro.
  
  
  METHODS
  Took enzyme concentration, time course and substrate concentration as investigation factors, used Box-Behnken design-response surface methodology to optimize the enzyme hydrolysis path of Arctigenin.
  
  
  RESULTS
  The best operational path for Arctigenin was as follows: the temperature was 50 degrees C, pH was 4.8, enzyme concentration was 0.44 U/mL, time course was 46.81 min, substrate concentration was 0.29 mg/mL, the conversion rate was 90.94%.
  
  
  CONCLUSIONS
  This research can be regarded as a referencein preparing Arctigenin in vitro.
authors.affiliation: Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. zlying0818@sina.com
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Zhang
authors.forename: Yi-Shun
authors.initials: YS
authors.lastname: Yang
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Yue
authors.initials: Y
authors.lastname: Ding
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Cai
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Tao
mesh_terms: D031170:Arctium / Q000737:chemistry*; D005419:Flavonoids / Q000378:metabolism*; D005663:Furans / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D017705:Lignans / Q000378:metabolism*; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature; D013997:Time Factors
chemical_list: D005419:Flavonoids; D005663:Furans; D005960:Glucosides; D017705:Lignans; C071942:arctigenin; C056599:icariin