title: Microfluidic flow fractionation device for label-free isolation of circulating tumor cells (CTCs) from breast cancer patients.
abstract: Circulating tumor cells (CTCs) are dissociated from primary tumor and circulate in peripheral blood. They are regarded as the genesis of metastasis. Isolation and enumeration of CTCs serve as valuable tools for cancer prognosis and diagnosis. However, the rarity and heterogeneity of CTCs in blood makes it difficult to separate intact CTCs without loss. In this paper, we introduce a parallel multi-orifice flow fractionation (p-MOFF) device in which a series of contraction/expansion microchannels are placed parallel on a chip forming four identical channels. CTCs were continuously isolated from the whole blood of breast cancer patients by hydrodynamic forces and cell size differences. Blood samples from 24 breast cancer patients were analyzed (half were from metastatic breast cancer patients and the rest were from adjuvant breast cancer patients). The number of isolated CTCs varied from 0 to 21 in 7.5 ml of blood. Because our devices do not require any labeling processes (e.g., EpCAM antibody), heterogeneous CTCs can be isolated regardless of EpCAM expression.
authors.affiliation: School of Mechanical Engineering, Yonsei University, 50 Yonsei-no Seodaemun-gu, Seoul 120-752, South Korea.
authors.forename: Kyung-A
authors.initials: KA
authors.lastname: Hyun
authors.forename: Kiho
authors.initials: K
authors.lastname: Kwon
authors.forename: Hyunju
authors.initials: H
authors.lastname: Han
authors.forename: Seung-Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Hyo-Il
authors.initials: HI
authors.lastname: Jung
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D005591:Chemical Fractionation / Q000295:instrumentation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*