title: Isolation of monoclonal antibody charge variants by displacement chromatography.
abstract: This unit discusses the important parameters in designing and optimizing a separation of monoclonal antibody (mAb) charge variants from process streams by ion-exchange displacement chromatography, including sample preparation and selection of matrix, column, and appropriate buffer. A protocol is provided for determination of optimal column binding and displacement conditions, including cleaning and regeneration of the displacement columns.
authors.affiliation: SACHEM, Inc, Austin, Texas.
authors.forename: C Patrick
authors.initials: CP
authors.lastname: McAtee
authors.affiliation: Emergent Biosolutions, Seattle, Washington.
authors.forename: Jacob
authors.initials: J
authors.lastname: Hornbuckle
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000302:isolation & purification*; D002411:Cation Exchange Resins / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal; D002411:Cation Exchange Resins