title: Development of a generic micellar electrokinetic chromatography method for the separation of 15 antimalarial drugs as a tool to detect medicine counterfeiting.
abstract: Since antimalarial drugs counterfeiting is dramatically present on the African market, the development of simple analytical methods for their quality control is of great importance. This work consists in the CE analysis of 15 antimalarials (artesunate, artemether, amodiaquine, chloroquine, piperaquine, primaquine, quinine, cinchonine, mefloquine, halofantrine, sulfadoxine, sulfalen, atovaquone, proguanil, and pyrimethamine). Since all these molecules cannot be ionized at the same pH, MEKC was preferred because it also allows separation of neutral compounds. Preliminary experiments were first carried out to select the most crucial factors affecting the antimalarials separation. Several conditions were tested and four parameters as well as their investigation domain were chosen: pH (5-10), SDS concentration (20-90 mM), ACN proportion (10-40%), and temperature (20-35°C). Then, the experimental design methodology was used and a central composite design was selected. Mathematical modeling of the migration times allowed the prediction of optimal conditions (29°C, pH 6.6, 29 mM SDS, 36% ACN) regarding analyte separation. The prediction at this optimum was verified experimentally and led to the separation of 13 compounds within 8 min. Finally, the method was successfully applied to the quality control of African antimalarial medicines for their qualitative and quantitative content.
authors.affiliation: Laboratory of Analytical Pharmaceutical Chemistry, Department of Pharmacy, CIRM, University of Liège, Belgium.
authors.forename: Caroline
authors.initials: C
authors.lastname: Lamalle
authors.forename: Roland
authors.initials: R
authors.lastname: Djang'Eing'A Marini
authors.forename: Benjamin
authors.initials: B
authors.lastname: Debrus
authors.forename: Pierre
authors.initials: P
authors.lastname: Lebrun
authors.forename: Jacques
authors.initials: J
authors.lastname: Crommen
authors.forename: Philippe
authors.initials: P
authors.lastname: Hubert
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Servais
authors.forename: Marianne
authors.initials: M
authors.lastname: Fillet
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000962:Antimalarials / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000592:standards; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D058110:Counterfeit Drugs / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D012107:Research Design; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D000962:Antimalarials; D058110:Counterfeit Drugs; D012967:Sodium Dodecyl Sulfate; C032159:acetonitrile