title: Simple and fast HPLC method for simultaneous determination of retinol, tocopherols, coenzyme Q(10) and carotenoids in complex samples.
abstract: The effects of fat-soluble vitamins (such as vitamins A and E) and lipid microconstituents (such as carotenoids) on human health are now well established. However, high-performance liquid chromatography (HPLC) methods able to detect these molecules in simultaneous runs are often difficult to set up. We report here a 35-min reversed-phase HPLC method using a single C30 column kept at 35°C with a gradient system of methanol, methyl-tert-butyl ether and water at a flow-rate of 1 mL/min. This method resolves 11 carotenoids, retinol, α- and γ-tocopherol from complex matrixes such as food samples, human plasma and human adipose tissue within 35 min. The method is also able to separate coenzyme Q(10). The intra-day and inter-day coefficients of variation are suitable for routine clinical and scientific applications for the determination of lipid micronutrients from various sample types.
authors.affiliation: INRA, UMR 1260 Nutriments Lipidiques et Prévention des Maladies Métaboliques, Marseille F-13385, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Gleize
authors.forename: Marlène
authors.initials: M
authors.lastname: Steib
authors.forename: Marc
authors.initials: M
authors.lastname: André
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Reboul
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D002338:Carotenoids / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods / Q000706:statistics & numerical data; D005504:Food Analysis; D006801:Humans; D024505:Tocopherols / Q000032:analysis* / Q000097:blood; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D014801:Vitamin A / Q000032:analysis* / Q000097:blood
chemical_list: D014801:Vitamin A; D014451:Ubiquinone; D002338:Carotenoids; C024989:coenzyme Q10; D024505:Tocopherols