title: The pathogenesis of acute allograft dysfunction in desensitized renal transplant recipients.
abstract: BACKGROUND
  Acute allograft rejection after HLA desensitization is common early post-transplant but the sequence of histopathologic changes leading to graft dysfunction has not been well defined.
  
  
  METHODS
  We evaluated the early pathogenesis and sequence of antibody-mediated graft damage of 35 desensitized living donor kidney recipients by studying the course of biopsies taken in the very early post-transplant period (<1 month).
  
  
  RESULTS
  A total of 14 of the 35 patients met criteria for acute antibody-mediated rejection (AMR). In these patients, the chronologic sequence of pathologic changes was C4d peritubular capillary deposition, acute tubular injury, and peritubular capillaritis, followed by glomerulitis and interstitial inflammation. Classic AMR lesions occurred early, followed by mononuclear cellular infiltration, which comprised CD4 and CD8 T cells and monocytes. Development of graft dysfunction in most patients occurred concurrently with the emergence of graft cellular infiltration, rather than at the earlier time of antibody deposition as detected via C4d deposition.
  
  
  CONCLUSIONS
  These data provide novel insight into the sequence of pathologic changes in patients with AMR post-transplant after HLA desensitization.
authors.affiliation: Department of Medicine, The Ohio State University College of Medicine and Public Health, Columbus, OH, USA.
authors.forename: N
authors.initials: N
authors.lastname: Singh
authors.forename: Q
authors.initials: Q
authors.lastname: Sun
authors.forename: T
authors.initials: T
authors.lastname: Nadasdy
authors.forename: P
authors.initials: P
authors.lastname: Adams
authors.forename: N R
authors.initials: NR
authors.lastname: Dipaola
authors.forename: T
authors.initials: T
authors.lastname: Pesavento
authors.forename: H
authors.initials: H
authors.lastname: Winters
authors.forename: A
authors.initials: A
authors.lastname: Satoskar
authors.forename: Z
authors.initials: Z
authors.lastname: Yu
authors.forename: M
authors.initials: M
authors.lastname: Henry
authors.forename: G A
authors.initials: GA
authors.lastname: Hadley
authors.forename: R P
authors.initials: RP
authors.lastname: Pelletier
mesh_terms: D003888:Desensitization, Immunologic; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006084:Graft Rejection / Q000276:immunology* / Q000473:pathology*; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000097:blood / Q000276:immunology*; D016030:Kidney Transplantation / Q000276:immunology* / Q000473:pathology*; D011379:Prognosis; D012189:Retrospective Studies; D014184:Transplantation, Homologous
chemical_list: D006680:HLA Antigens; D007518:Isoantibodies