title: Single nucleotide analysis of cytosine methylation by whole-genome shotgun bisulfite sequencing.
abstract: Unlike other methods to assess methylation, whole-genome shotgun bisulfite sequencing is used to generate quantitative genome-wide methylation profiles at single-nucleotide resolution. As described in this unit, this method allows for the quantitative measurement of methylation at each cytosine base by treatment of genomic DNA with sodium bisulfite followed by sequencing and alignment of the reads to a reference genome.
authors.affiliation: National Heart & Lung Institute, Imperial College London, London, United Kingdom.
authors.forename: Michelle D
authors.initials: MD
authors.lastname: Johnson
authors.forename: Michael
authors.initials: M
authors.lastname: Mueller
authors.forename: Laurence
authors.initials: L
authors.lastname: Game
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Aitman
mesh_terms: D003596:Cytosine / Q000378:metabolism*; D019175:DNA Methylation; D015723:Gene Library; D005821:Genetic Techniques; D016678:Genome; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D013447:Sulfites / Q000737:chemistry
chemical_list: D013447:Sulfites; D003596:Cytosine; C009279:sodium bisulfite