title: [Roles and mechanisms of E2F-1 and PAC1 in signaling oxidative stress-induced apoptosis of Saos-2 cells].
abstract: OBJECTIVE
  To explore the roles and mechanisms of E2F-1 and PAC1 in signaling apoptosis of Saos-2 cells under oxidative stress.
  
  
  METHODS
  siRNAs were used to construct the cell clones of expressing siE2F-1 and siPAC1. E2F-1/PAC1-mediated induction of apoptotic cell death in response to H2O2 were examined by trypan blue exclusion and the expression levels of related target factors detected by Western blot.
  
  
  RESULTS
  The cell viabilities of Saos-2/siE2F-1 and Saos-2/siPAC1 increased markedly comparing with the control cells after the treatment of H2O2. And the expression level of p-ERK1/2 was higher than that of the control cells.
  
  
  CONCLUSIONS
  The pathway of E2F-1/PAC1/MAPKase is a specific cascade for apoptotic signaling.
authors.affiliation: Department of Oncology, First Affiliated hospital, Zhengzhou University, Henan 450052, China.
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Wu
authors.forename: Hui-jie
authors.initials: HJ
authors.lastname: Fan
authors.forename: Qing-xia
authors.initials: QX
authors.lastname: Fan
authors.forename: Ming-zhi
authors.initials: MZ
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D054641:Dual Specificity Phosphatase 2 / Q000235:genetics / Q000378:metabolism*; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress; D010766:Phosphorylation; D034741:RNA, Small Interfering
chemical_list: D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human; D034741:RNA, Small Interfering; D054641:Dual Specificity Phosphatase 2