title: Photoinduced upregulation of calcitonin gene-related peptide in A549 cells through HNO release from a hydrophilic photocontrollable HNO donor.
abstract: Nitroxyl (HNO), a one-electron-reduced form of nitric oxide, has various biological activities, including a cardioprotective effect. Here, we first synthesized another, more hydrophilic photocontrollable HNO donor (3), which can release HNO in a spatially and temporally controlled manner, and then examined the properties of our series of compounds as practical HNO donors in a cellular system under photocontrol. We selected compound 2 as the preferred donor, and used it to show that calcitonin gene-related peptide (CGRP) can be upregulated in A549 cells via photocontrolled HNO release. This result demonstrates the suitability of this photocontrollable HNO donor for biological investigations.
authors.affiliation: Department of Organic and Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Nagoya, Aichi 467-8603, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Matsuo
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Nakagawa
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Adachi
authors.forename: Eri
authors.initials: E
authors.lastname: Kameda
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Aizawa
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tsumoto
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Naoki
authors.initials: N
authors.lastname: Miyata
mesh_terms: D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002460:Cell Line; D002849:Chromatography, Gas; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009589:Nitrogen Oxides / Q000494:pharmacology*; D055668:Photochemical Processes; D016339:Spectrometry, Mass, Fast Atom Bombardment; D015854:Up-Regulation
chemical_list: D009589:Nitrogen Oxides; C039900:nitroxyl; D015740:Calcitonin Gene-Related Peptide