title: Germ cell specification.
abstract: The germline of Caenorhabditis elegans derives from a single founder cell, the germline blastomere P(4). P(4) is the product of four asymmetric cleavages that divide the zygote into distinct somatic and germline (P) lineages. P(4) inherits a specialized cytoplasm ("germ plasm") containing maternally encoded proteins and RNAs. The germ plasm has been hypothesized to specify germ cell fate, but the mechanisms involved remain unclear. Three processes stand out: (1) inhibition of mRNA transcription to prevent activation of somatic development, (2) translational regulation of the nanos homolog nos-2 and of other germ plasm mRNAs, and (3) establishment of a unique, partially repressive chromatin. Together, these processes ensure that the daughters of P(4), the primordial germ cells Z2 and Z3, gastrulate inside the embryo, associate with the somatic gonad, initiate the germline transcriptional program, and proliferate during larval development to generate ∼2,000 germ cells by adulthood.
authors.affiliation: Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Jennifer T
authors.initials: JT
authors.lastname: Wang
authors.forename: Geraldine
authors.initials: G
authors.lastname: Seydoux
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000254:growth & development*; D019070:Cell Lineage; D047108:Embryonic Development; D005854:Germ Cells / Q000166:cytology*