title: Fast determination of bioactive compounds from Lycopersicon esculentum Mill. leaves.
abstract: Lycopersicon esculentum leaves, usually considered as a by-product of tomato production, present several bioactive compounds of interest for industries like food, pharmaceutical and cosmetics. Nevertheless, before industrial application, suitable methods to identify and quantify those metabolites should be developed. In this study agitation with aqueous methanol was used for phenolic compounds extraction. Solid-phase extraction (SPE) was performed as the purification step before alkaloids analysis. Among the SPE sorbents tested, sulphonic acid bonded silica with H(+) counterion (SCX) proved to be the most efficient one for removing interfering components. Fifteen phenolics and four steroidic alkaloids were identified in 35 and 20 min analysis, respectively. The optimised methods were validated, revealing to be accurate, fast, simple and sensitive. Thus, these methods represent an easy and fast analytical approach, using equipment available in almost laboratory, which render them to be appropriate for routine analysis.
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, Portugal.
authors.forename: Marcos
authors.initials: M
authors.lastname: Taveira
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Angel
authors.initials: A
authors.lastname: Gil-Izquierdo
authors.forename: Luísa
authors.initials: L
authors.lastname: Oliveira
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D018551:Solanum lycopersicum / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D000470:Alkaloids; D010636:Phenols; D010936:Plant Extracts