title: [Transplantation of autologous myoblasts transfected with VEGF165 improves heart function after myocardial infarction in rabbits].
abstract: OBJECTIVE
  To determine the improvements of post-infarction heart function after transplantation of autologous skeletal myoblasts transfected with VEGF165 in rabbits.
  
  
  METHODS
  Myocardium infarction was induced in rabbits by left anterior descending coronary artery ligation. At 2 weeks, 1.75×10(7) autologous skeletal myoblasts transfected with pcDNA3.1-VEGF165 were infused into the region of MI via direct intramuscular injection; pcDNA3.1 served as a control.
  
  
  RESULTS
  The DAPI-labeled and Desmin-positive immunostained skeletal myofibers were found throughout the infracted areas and border zones, and the density of blood capillary in the MI region transplanted by myoblasts with VEGF165 was increased (measured 4 weeks later and compared with controls). Heart function was examined by the Buxco system and demonstrated that maximum dp/dt [(1607.23±102.67) mmHg/s vs (1217.77±89.91) mmHg/s] and minimum dp/dt [(-1535.09 ± 81.34) mmHg/s vs (1174.58 ± 91.5) mmHg/s] were improved in the heart transplanted with the transfected myoblasts(P<0.05).
  
  
  CONCLUSIONS
  Autologous skeletal myoblasts transfected with VEGF165 could ameliorate the blood supply in the MI region, and aid recovery of heart function more quickly in post-infarction hearts. This suggests an effective treatment for myocardium infarction.
authors.affiliation: Department of Cardiothoracic Surgery, Central South University, Changsha, China. zhuangweihy@sina.com.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Zhuang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Qingchun
authors.initials: Q
authors.lastname: Zhang
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Lin
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Deng
mesh_terms: D000818:Animals; D005260:Female; D032448:Myoblasts, Skeletal / Q000378:metabolism / Q000637:transplantation*; D009203:Myocardial Infarction / Q000503:physiopathology* / Q000628:therapy*; D011817:Rabbits; D020127:Recovery of Function; D014162:Transfection; D014182:Transplantation, Autologous; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism; D016277:Ventricular Function, Left / Q000502:physiology*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A