title: Non-ionic polysorbate surfactants: alternative inducers of medium-chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) for production of extracellular MCL-PHA depolymerases.
abstract: The potential of non-ionic polysorbate surfactants as alternative inducers of medium-chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) for the production of diverse bacterial MCL-PHA depolymerases was evaluated. When grown with corn oil as the sole carbon substrate, Pseudomonas alcaligenes LB19 preferentially produced lipolytic enzymes, but its MCL-PHA depolymerase was not induced by the substrate. However, the results of activity staining and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis clearly revealed that Tween 20 induced simultaneous production of lipolytic enzymes and the MCL-PHA depolymerase with the molecular mass (26.5 kDa) of P. alcaligenes LB19, which has been previously identified. Moreover, the co-production of two functionally distinct hydrolytic enzymes induced by Tween 20 was commonly observed in various Gram-positive and Gram-negative bacteria that were fed the substrate. Thus, it is expected that non-ionic polysorbate surfactants including Tween 20 can be widely exploited as promising universal substrates for the facile and efficient production of diverse MCL-PHA depolymerases.
authors.affiliation: Department of Microbiology and Molecular Biology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Bong Su
authors.initials: BS
authors.lastname: Seo
authors.forename: Do Young
authors.initials: DY
authors.lastname: Kim
authors.forename: Yu-Yang
authors.initials: YY
authors.lastname: Ni
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Son
authors.forename: Ho-Yong
authors.initials: HY
authors.lastname: Park
authors.forename: Young Ha
authors.initials: YH
authors.lastname: Rhee
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D054813:Polyhydroxyalkanoates / Q000378:metabolism*; D011136:Polysorbates / Q000378:metabolism*; D044202:Pseudomonas alcaligenes / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D054813:Polyhydroxyalkanoates; D011136:Polysorbates; D013501:Surface-Active Agents; D002265:Carboxylic Ester Hydrolases; C077226:poly(3-hydroxyalkanoic acid) depolymerase