title: High-throughput sequencing of small RNAs and analysis of differentially expressed microRNAs associated with pistil development in Japanese apricot.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are a class of endogenous, small, non-coding RNAs that regulate gene expression by mediating gene silencing at transcriptional and post-transcriptional levels in high plants. However, the diversity of miRNAs and their roles in floral development in Japanese apricot (Prunus mume Sieb. et Zucc) remains largely unexplored. Imperfect flowers with pistil abortion seriously decrease production yields. To understand the role of miRNAs in pistil development, pistil development-related miRNAs were identified by Solexa sequencing in Japanese apricot.
  
  
  RESULTS
  Solexa sequencing was used to identify and quantitatively profile small RNAs from perfect and imperfect flower buds of Japanese apricot. A total of 22,561,972 and 24,952,690 reads were sequenced from two small RNA libraries constructed from perfect and imperfect flower buds, respectively. Sixty-one known miRNAs, belonging to 24 families, were identified. Comparative profiling revealed that seven known miRNAs exhibited significant differential expression between perfect and imperfect flower buds. A total of 61 potentially novel miRNAs/new members of known miRNA families were also identified by the presence of mature miRNAs and corresponding miRNA*s in the sRNA libraries. Comparative analysis showed that six potentially novel miRNAs were differentially expressed between perfect and imperfect flower buds. Target predictions of the 13 differentially expressed miRNAs resulted in 212 target genes. Gene ontology (GO) annotation revealed that high-ranking miRNA target genes are those implicated in the developmental process, the regulation of transcription and response to stress.
  
  
  CONCLUSIONS
  This study represents the first comparative identification of miRNAomes between perfect and imperfect Japanese apricot flowers. Seven known miRNAs and six potentially novel miRNAs associated with pistil development were identified, using high-throughput sequencing of small RNAs. The findings, both computationally and experimentally, provide valuable information for further functional characterisation of miRNAs associated with pistil development in plants.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, P R China. gaozhihong@njau.edu.cn
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Gao
authors.forename: Ting
authors.initials: T
authors.lastname: Shi
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Luo
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhuang
authors.forename: Liangju
authors.initials: L
authors.lastname: Wang
mesh_terms: D019295:Computational Biology; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing; D007564:Japan; D035683:MicroRNAs / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D027861:Prunus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018749:RNA, Plant / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant