title: Isolation and characterization of microsatellite markers for Thujopsis dolabrata var. hondai (Cupressaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for the endemic Japanese species Thujopsis dolabrata var. hondai for studies on forest ecology and tree breeding.
  
  
  RESULTS
  We characterized 10 dinucleotide microsatellite loci by screening primers developed using a simple sequence repeat-enriched library. The number of alleles per locus ranged from eight to 44 with an average of 20.3. The observed and expected heterozygosities ranged from 0.326 to 0.854 and from 0.670 to 0.976, respectively.
  
  
  CONCLUSIONS
  The development of these markers can be used to assess useful genetic information for ecological studies and tree breeding in T. dolabrata var. hondai.
authors.affiliation: Institute of Wood Technology, Akita Prefectural University, Noshiro, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Mishima
authors.forename: Tomonori
authors.initials: T
authors.lastname: Hirao
authors.forename: Atsushi
authors.initials: A
authors.lastname: Watanabe
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Takata
mesh_terms: D000483:Alleles; D001483:Base Sequence; D029779:Cupressaceae / Q000145:classification / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D015723:Gene Library; D056426:Genetic Loci; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers