title: JNK/FOXO mediated PeroxiredoxinV expression regulates redox homeostasis during Drosophila melanogaster gut infection.
abstract: Innate immunity plays an important role in combating microbial infection in animals. During bacterial infection in Drosophila melanogaster gut, Dual oxidase (Duox) generates reactive oxygen species (ROS) to fight against the infected microbes. Concurrently, antioxidant systems eliminate residual ROS and protect the hosts. Here we found that Drosophila melanogaster Peroxiredoxin V (dPrxV) is an immune-related antioxidant enzyme which maintains intestinal redox homeostasis. dPrxV was highly expressed in gut and induced by the oral infection of Erwinia carotovora carotovora. dPrxV expression was increased by the gut-specific Duox overexpression but decreased by Duox inhibition. Moreover, dPrxV expression was mediated by the JNK/FOXO signaling and dPrxV mutant reduced survival after gut infection. These results suggest that JNK/FOXO mediated dPrxV expression plays a critical role in Drosophila melanogaster gut during bacterial infection in protecting the host gut epithelial cells from oxidative damage.
authors.affiliation: Aging Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Hye-Mi
authors.initials: HM
authors.lastname: Ahn
authors.forename: Kyu-Sun
authors.initials: KS
authors.lastname: Lee
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Lee
authors.forename: Kweon
authors.initials: K
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D029721:Drosophila Proteins / Q000276:immunology / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D051858:Forkhead Transcription Factors / Q000378:metabolism; D041981:Gastrointestinal Tract / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D006706:Homeostasis; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008969:Molecular Sequence Data; D019255:NADPH Oxidases / Q000378:metabolism; D018384:Oxidative Stress / Q000276:immunology; D016973:Pectobacterium carotovorum / Q000276:immunology / Q000472:pathogenicity; D054464:Peroxiredoxins / Q000737:chemistry / Q000378:metabolism*; D017382:Reactive Oxygen Species; D016415:Sequence Alignment; D015398:Signal Transduction / Q000276:immunology
chemical_list: D000975:Antioxidants; D029721:Drosophila Proteins; C477334:FOXO protein, Drosophila; D051858:Forkhead Transcription Factors; D017382:Reactive Oxygen Species; C505384:dual oxidase, Drosophila; D054464:Peroxiredoxins; D019255:NADPH Oxidases; D048031:JNK Mitogen-Activated Protein Kinases