title: Surface engineering of nanoparticles in suspension for particle based bio-sensing.
abstract: Surface activation of nanoparticles in suspension using amino organosilane has been carried out via strict control of a particle surface ad-layer of water using a simple but efficient protocol 'Tri-phasic Reverse Emulsion' (TPRE). This approach produced thin and ordered layers of particle surface functional groups which allowed the efficient conjugation of biomolecules. When used in bio-sensing applications, the resultant conjugates were highly efficient in the hybrid capture of complementary oligonucleotides and the detection of food borne microorganism. TPRE overcomes a number of fundamental problems associated with the surface modification of particles in aqueous suspension viz. particle aggregation, density and organization of resultant surface functional groups by controlling surface condensation of the aminosilane. The approach has potential for application in areas as diverse as nanomedicine, to food technology and industrial catalysis.
authors.affiliation: Nanobiotechnology Research Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, United Kingdom. tsen@uclan.ac.uk
authors.forename: Tapas
authors.initials: T
authors.lastname: Sen
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Bruce
mesh_terms: D000588:Amines; D015374:Biosensing Techniques / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009841:Oligonucleotides / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties; D013535:Suspensions
chemical_list: D000588:Amines; D058185:Magnetite Nanoparticles; D009841:Oligonucleotides; D013535:Suspensions; D012825:Silicon