title: Halloysite clay nanotubes for resveratrol delivery to cancer cells.
abstract: Halloysite is natural aluminosilicate clay with hollow tubular structure which allows loading with low soluble drugs using their saturated solutions in organic solvents. Resveratrol, a polyphenol known for having antioxidant and antineoplastic properties, is loaded inside these clay nanotubes lumens. Release time of 48 h is demonstrated. Spectroscopic and ζ-potential measurements are used to study the drug loading/release and for monitoring the nanotube layer-by-layer (LbL) coating with polyelectrolytes for further release control. Resveratrol-loaded clay nanotubes are added to breast cell cultures for toxicity tests. Halloysite functionalization with LbL polyelectrolyte multilayers remarkably decrease nanotube self-toxicity. MTT measurements performed with a neoplastic cell lines model system (MCF-7) as function of the resveratrol-loaded nanotubes concentration and incubation time indicate that drug-loaded halloysite strongly increase of cytotoxicity leading to cell apoptosis.
authors.affiliation: NNL-Istituto Nanoscienze, CNR Via Arnesano 16 Lecce, 73100, Italy.
authors.forename: Viviana
authors.initials: V
authors.lastname: Vergaro
authors.forename: Yuri M
authors.initials: YM
authors.lastname: Lvov
authors.forename: Stefano
authors.initials: S
authors.lastname: Leporatti
mesh_terms: D000538:Aluminum Silicates; D000975:Antioxidants / Q000008:administration & dosage* / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D000077215:Clay; D006801:Humans; D007700:Kinetics; D061986:MCF-7 Cells; D046529:Microscopy, Electron, Transmission; D043942:Nanotubes; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage* / Q000627:therapeutic use
chemical_list: D000538:Aluminum Silicates; D000975:Antioxidants; D013267:Stilbenes; D000077185:Resveratrol; D000077215:Clay