title: Distinction of broken cellular wall Ganoderma lucidum spores and G. lucidum spores using FTIR microspectroscopy.
abstract: In this paper, FTIR microspectroscopy was used to identify broken cellular wall Ganoderma lucidum spores and G. lucidum spores. For IR spectra, broken cellular wall G. lucidum spores and G. lucidum spores were mainly different in the regions of 3000-2800, 1660-1600, 1400-1200 and 1100-1000 cm(-1). For curve fitting, the results showed the differences in the protein secondary structures and the polysaccharide structures/content between broken cellular wall G. lucidum spores and G. lucidum spores. Moreover, the value of A1078/A1741 might be a potentially useful factor to distinguish broken cellular wall G. lucidum spores from G. lucidum spores. Additionally, FTIR microspectroscopy could identify broken cellular wall G. lucidum spores and G. lucidum spores accurately when it was combined with hierarchical cluster analysis. The result suggests FTIR microspectroscopy is very simple and efficient for distinction of broken cellular wall G. lucidum spores and G. lucidum spores. The result also indicates FTIR microspectroscopy may be useful for TCM identification.
authors.affiliation: College of Basic Medicine, Anhui Medical University, Hefei, Anhui 230032, China.
authors.forename: Xianliang
authors.initials: X
authors.lastname: Chen
authors.forename: Xingcun
authors.initials: X
authors.lastname: Liu
authors.forename: Daping
authors.initials: D
authors.lastname: Sheng
authors.forename: Dake
authors.initials: D
authors.lastname: Huang
authors.forename: Weizu
authors.initials: W
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000042:Absorption; D002473:Cell Wall / Q000378:metabolism*; D016000:Cluster Analysis; D015591:Microspectrophotometry; D020940:Reishi / Q000166:cytology*; D017550:Spectroscopy, Fourier Transform Infrared; D013172:Spores, Fungal / Q000166:cytology*