title: Coupling technique of self-ordered ring and phosphorimetry for the determination of alkaline phosphatase and diseases prediction.
abstract: Rhodamine S could emit strong and stable room temperature phosphorescence (RTP) on polyamide membrane (PAM) in the presence of heavy atom perturber Pb(2+). When Rhodamine S-piperidine solution was dropped on PAM, the red (Rhod.S)(n)-P-SOR (Rhod.S, (Rhod.S)(n), P and SOR refer to alizarin red S, multiple Rhod.S molecules, piperidine and self-ordered ring, respectively) formed on PAM, leading to the enhancement of room temperature phosphorimetry (RTP) intensity (I(p), 117.2) of (Rhod.S)(n)-P-SOR system, which was 2.4 times higher than that without SOR (I(p), 48.1). Wheat germ agglutinin (WGA) was labelled with (Rhod.S)(n)-P-SOR by the -NH- of Rhod.S reacting with the -COOH of WGA to form WGA-(Rhod.S)(n)-P-SOR. The formation of WGA-AP-WGA-(Rhod.S)(n)-P-SOR in the affinity adsorption (AA) reaction carried out between the -COOH of WGA in WGA-(Rhod.S)(n)-P-SOR and the -NH(2) of alkaline phosphatase (AP) caused the RTP intensity (ΔI(p)) of the WGA-AP-WGA-(Rhod.S)(n)-P-SOR system 7.8 times larger than that without (Rhod.S)(n)-P-SOR. Therefore, the coupling technique of SOR and solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace AP has been established. This method possessed good selectivity, high sensitivity (Detection limit (L.D) was 3.4×10(-16)gmL(-1)) and accuracy, and it has been applied to the determination of trace AP in human serum and the forecast of human diseases, and the results agreed well with those obtained by enzyme-linked immunoassay (ELISA). Besides, the mechanism of the coupling technique for the determination of AP was discussed.
authors.affiliation: Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou 363000, PR China.
authors.forename: Li Hong
authors.initials: LH
authors.lastname: Zhang
authors.forename: Zhi Yong
authors.initials: ZY
authors.lastname: Zheng
authors.forename: Shu-Lian
authors.initials: SL
authors.lastname: Jiang
authors.forename: Ma-Lin
authors.initials: ML
authors.lastname: Cui
authors.forename: Li
authors.initials: L
authors.lastname: Jiao
authors.forename: Xuan
authors.initials: X
authors.lastname: Lin
authors.forename: Wen-Lian
authors.initials: WL
authors.lastname: Cai
authors.forename: Shao-Qin
authors.initials: SQ
authors.lastname: Lin
authors.forename: Jia-Ming
authors.initials: JM
authors.lastname: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood*; D000880:Anthraquinones / Q000737:chemistry / Q000378:metabolism; D002021:Buffers; D004194:Disease; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D010880:Piperidines / Q000737:chemistry / Q000378:metabolism; D012235:Rhodamines / Q000737:chemistry / Q000378:metabolism; D012997:Solvents; D013379:Substrate Specificity; D013696:Temperature; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D000880:Anthraquinones; D002021:Buffers; D010880:Piperidines; D012235:Rhodamines; D012997:Solvents; D014909:Wheat Germ Agglutinins; C004468:Alizarin Red S; C032727:piperidine; D000469:Alkaline Phosphatase