title: Enterocytozoon bieneusi in Bovine Viral Diarrhea Virus (BVDV) infected and noninfected cattle herds.
abstract: Enterocytozoon bieneusi known as a causative agent of opportunistic infections instigating diarrhoea in AIDS patients was identified also in a number of immunocompetent patients and in a wide range of animals, including cattle. In the present study we tested if the Bovine Viral Diarrhea Virus (BVDV), the most common pathogen underlying immunosuppressive Bovine Viral Diarrhoea (BVD), can enhance the occurrence of opportunistic infections with E. bieneusi in cattle. Six dairy farms were investigated using ELISA to detect antibodies against or antigens arising from BVDV in collected sera. A total of 240 individual faecal samples from four age groups were examined for the presence of E. bieneusi by nested PCR. Sequence analysis of six E. bieneusi positive samples revealed the presence of the genotype I of E. bieneusi, previously described in cattle. The hypothesis expecting higher prevalence of E. bieneusi in BVDV positive cattle herds was not confirmed in this study; however this is the first description about E. bieneusi in cattle in the Czech Republic.
authors.affiliation: Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic. yanniska@seznam.cz
authors.forename: J
authors.initials: J
authors.lastname: Juránková
authors.forename: M
authors.initials: M
authors.lastname: Kamler
authors.forename: K
authors.initials: K
authors.lastname: Kovařčík
authors.forename: B
authors.initials: B
authors.lastname: Koudela
mesh_terms: D000367:Age Factors; D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000150:complications* / Q000382:microbiology / Q000821:virology; D002417:Cattle / Q000469:parasitology / Q000821:virology; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D018153:Czech Republic / Q000453:epidemiology; D001908:Diarrhea Viruses, Bovine Viral; D021862:Enterocytozoon / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005260:Female; D005838:Genotype; D016881:Microsporidiosis / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D017422:Sequence Analysis, DNA / Q000662:veterinary