title: Novel pHEMA-gelatin SPHs as bone scaffolds in dynamic cultures.
abstract: The effectiveness of poly(2-hydroxyethyl methacrylate)-gelatin superporous hydrogels (pHEMA-gelatin SPHs) was investigated for bone tissue engineering. The cell culture studies were performed with preosteoblastic MC3T3-E1 cells. Dynamic culture conditions were provided using 100 ml spinner flask rotating at 50 rpm. According to the results of mitochondrial activity test (1-3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium bromide), there is no significant difference between proliferation behavior of cells cultured under static and dynamic conditions during 28 days. Observations by scanning electron microscopy and confocal laser scanning microscopy showed that, cells attached well onto the scaffolds and spread through the pores for both culture conditions. However, it was found that, calcium deposition and alkaline phosphatase activity in the scaffolds cultured under dynamic conditions were higher than that of static conditions. The expression of osteogenic differentiation markers, i.e. collagen I and osteopontin, based on real-time reverse transcriptase-polymerase chain reaction demonstrated increased responses under the spinner flask culture conditions. The combination of dynamic culture conditions in spinner flask with the use of superporous pHEMA-gelatin scaffolds enhanced the outcomes related to bone tissue engineering.
authors.affiliation: Chemical Engineering Department, Hacettepe University, Ankara, Turkey.
authors.forename: Damla
authors.initials: D
authors.lastname: Cetin
authors.forename: A Sera
authors.initials: AS
authors.lastname: Kahraman
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001842:Bone and Bones; D002113:Calcification, Physiologic; D017931:DNA Primers; D005780:Gelatin / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D017931:DNA Primers; D011102:Polyhydroxyethyl Methacrylate; D005780:Gelatin; D000469:Alkaline Phosphatase