title: [Study on HPLC fingerprint of Herba Lysimachiae].
abstract: OBJECTIVE
  To establish the chromatographic fingerprint for Herba Lysimachiae by RP-HPLC.
  
  
  METHODS
  The sample solutions were prepared by extracting material with boiling water,followed by extracting with BuOH from the aqueous extract and analyzed by RP-HPLC with a Phenomenex C18 column (4.6 mm x 150 mm, 5 microm), gradiently eluted with A (acetonitrile containing 0.5% of acetic acid)-B (water containing 0.5% of acetic acid) at 30 degrees C, and monitored with UV detector at 255 nm. The flow rate was 1.0 mL/min, and sample volume injected was 20 microL. The chromatograms of different batches of Herba Lysimachiae were compared by the software of Similarity Evaluation System for Chromatographic Fingerprint of TCM (Version 2004A).
  
  
  RESULTS
  HPLC Fingerprint of Herba Lysimachiae was established with Rutin as the reference compound. 13 common peaks were selected as the fingerprint peaks of Herba Lysimachiae.
  
  
  CONCLUSIONS
  The established HPLC fingerprint of Herba Lysimachiae has desirable precision, reproducibility, and can be applied to routine quality control of Herba Lysimachiae.
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China. happydaygoodluck1234@126.com
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Guo
authors.forename: De
authors.initials: D
authors.lastname: Zhao
authors.forename: Jun
authors.initials: J
authors.lastname: Deng
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D010946:Plants, Medicinal / Q000737:chemistry*; D029623:Primulaceae / Q000737:chemistry* / Q000145:classification; D011786:Quality Control; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D012997:Solvents