title: Expression and significance of Kruppel-like factor 6 gene in osteosarcoma.
abstract: OBJECTIVE
  Osteosarcoma is primary malignant tumour of bone. Kruppel-like factor 6 (KLF6) is a tumor suppressor gene frequently inactivated in a number of human cancers and a ubiquitously expressed zinc-finger transcription factor. The present study aimed to first explore the relationship between the expression level of the KLF6 gene in osteosarcoma and the occurrence of bone tumours.
  
  
  METHODS
  KLF6 mRNA and protein expression levels in osteosarcoma and normal bone tissue were assayed by real-time quantitative PCR and immunohistochemistry. KLF6 mRNA and protein expression levels in osteosarcoma cells and normal osteoblasts were detected by semi-quantitative reverse transcription PCR and Western blotting, respectively.
  
  
  RESULTS
  Both the expression of KLF6 mRNA and protein in osteosarcoma cells and tissues were significantly lower than that in normal cells and tumour-adjacent tissues.
  
  
  CONCLUSIONS
  KLF6 is a putative tumor suppressor gene involved in osteosarcoma which can be used as a new therapeutic target and an important marker for early diagnosis and postoperative monitoring.
authors.affiliation: Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiao-dong
authors.initials: XD
authors.lastname: Zhu
authors.forename: Yu-shu
authors.initials: YS
authors.lastname: Bai
authors.forename: Xian-zhao
authors.initials: XZ
authors.lastname: Wei
authors.forename: Chuan-feng
authors.initials: CF
authors.lastname: Wang
authors.forename: Zi-qiang
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D001859:Bone Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002648:Child; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012516:Osteosarcoma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; C105465:KLF6 protein, human; D000075723:Kruppel-Like Factor 6; D051741:Kruppel-Like Transcription Factors; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger