title: Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell.
abstract: Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) with the use of a slide-on germanium accessory followed by chemometric analysis allowed for providing meaningful information about the biochemical composition of a single endothelial cell. In this work, the methodology of the ATR-FTIR measurements of dried cells and dried cells immersed in water solution is presented. The contact of the cell and Ge crystal was set up manually and monitored through the integration of the amide I band. Additionally, the cell imaging in transreflection mode was tested, but the spectral differences between sub-cellular structures were not prominent in the registered spectra. It has been shown that the ATR-FTIR method gives better results due to the increased spatial resolution and S/R ratio as well as small contribution of the optical artifacts.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, Krakow, Poland.
authors.forename: Tomasz P
authors.initials: TP
authors.lastname: Wrobel
authors.forename: Katarzyna M
authors.initials: KM
authors.lastname: Marzec
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Majzner
authors.forename: Kamila
authors.initials: K
authors.lastname: Kochan
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bartus
authors.forename: Stefan
authors.initials: S
authors.lastname: Chlopicki
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Baranska
mesh_terms: D002460:Cell Line; D042783:Endothelial Cells / Q000737:chemistry / Q000166:cytology*; D005857:Germanium; D006801:Humans; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D005857:Germanium