title: In vitro evaluation of combined hyperthermia and photodynamic effects using magnetoliposomes loaded with cucurbituril zinc phthalocyanine complex on melanoma.
abstract: The aims of this study were two fold; to develop magnetoliposomes (MLs) loaded with zinc phthalocyanine (ZnPc) complexed with cucurbituril (CB) (CB:ZnPc-MLs) and to evaluate their in vitro photodynamic (PD) and/or hyperthermia (HT) effects while using melanoma cells (B16-F10) as model. The liposomal formulations were characterized by both average diameter and zeta potential. The vesicle average size ranged from 150 to 200 nm and the polydispersity index (PdI) from 0.093 to 0.230. The zeta potential was significantly positive with values between 48 and 57 mV. The cell viability (CV) after PD and HT treatments was assessed by colorimetric MTT method. Melanoma cells were initially treated with the liposome formulation without light and magnetic field application, revealing cell viability not different from the control cells (p>0.05). Photodynamic and hyperthermia assays were also applied separately, demonstrating that PD is more effective than HT in reducing the CV of the neoplastic cells. Combined application of both PD and HT treatments was even more effective in reducing the CV of B16-F10 cells. At the highest light dose (2 J/cm(2)) and under magnetic field activation the CV was about half than PD applied alone. Therefore, the use of the photosensitizer-loaded magnetoliposome for combined photodynamic therapy (PDT) and magnetohyperthermia (MHT) application can be considered as a potential tool to treat malignant melanoma.
authors.affiliation: Departamento de Química, Laboratório de Fotobiologia e Fotomedicina, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP), Universidade de São Paulo, Av. dos Bandeirantes 3900, Vila Monte Alegre, Ribeirão Preto SP 14040-901, Brazil.
authors.forename: Gisele C
authors.initials: GC
authors.lastname: Bolfarini
authors.forename: Marigilson P
authors.initials: MP
authors.lastname: Siqueira-Moura
authors.forename: Gregoire J F
authors.initials: GJ
authors.lastname: Demets
authors.forename: Paulo C
authors.initials: PC
authors.lastname: Morais
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Tedesco
mesh_terms: D000818:Animals; D001952:Bridged-Ring Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D005290:Ferric Compounds / Q000737:chemistry; D006979:Hyperthermia, Induced / Q000379:methods*; D007093:Imidazoles / Q000737:chemistry*; D007211:Indoles / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D054833:Isoindoles; D008027:Light; D008081:Liposomes; D060526:Magnetic Fields; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D053758:Nanoparticles / Q000737:chemistry; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D017967:Zinc Compounds; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
chemical_list: D001952:Bridged-Ring Compounds; D005290:Ferric Compounds; D007093:Imidazoles; D007211:Indoles; D054833:Isoindoles; D008081:Liposomes; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C456276:cucurbit(7)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils; C052159:Zn(II)-phthalocyanine; C000499:ferric oxide