title: Detection of silver(I) ions based on the controlled self-assembly of a perylene fluorescence probe.
abstract: In the current work, we report a label-free fluorescence turn-on approach for the sensitive and selective sensing of Ag(+). A cationic perylene derivative, compound A, was used as the fluorescence probe. Compound A monomer is strongly fluorescent, and the fluorescence can be efficiently quenched through self-aggregation (self-assembly). A cytosine (C)-rich oligonucleotide, oligo-C, was employed. In the absence of Ag(+), oligo-C induced strong compound A aggregation due to electrostatic interactions in aqueous media, and very weak fluorescence signal was detected. However, in the presence of Ag(+), the specific interactions between oligo-C and Ag(+) induced hairpin structure formation of oligo-C through C-Ag(+)-C bonding interactions. Oligo-C binding to compound A aggregates was weakened; therefore, compound A monomer could be released and detected. The intensity of the fluorescence signal was directly related to the amount of Ag(+) added to the assay solution. Our method is highly sensitive-a limit of detection of 5nM was obtained-and also very selective. Ag(+) detection in complex sample mixtures was also demonstrated.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, People's Republic of China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yang
authors.forename: Wenying
authors.initials: W
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Qi
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Shujie
authors.initials: S
authors.lastname: Wang
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003596:Cytosine / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D055029:Inverted Repeat Sequences; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D010569:Perylene / Q000737:chemistry*; D012834:Silver / Q000032:analysis* / Q000737:chemistry; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014867:Water / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D009841:Oligonucleotides; D014867:Water; D012834:Silver; D010569:Perylene; D003596:Cytosine