title: The occurrence of genetic polymorphism and related non-allelic proteins increases the compositional complexity of goat α((s1)) -CN.
abstract: A genetic survey on three autochthonous goat breeds reared in Italy was carried out by a proteomic approach. This methodology, further to providing the phenotypic frequency of identified α(s1) genetic variants, allowed to determine (i) the additional constitutive presence of a non-allelic 'α(s1) -casein (CN) F like' protein in goat 'strong' α(s1) variants; (ii) an α(s1) -CN B(2) like protein, expressed at very low quantitative level, in goat 'weak' α(s1) -CN variants, and, as main focus; (iii) the occurrence of a new α(s1) -CN D(1) variant characterised by the lack of α(s1) (f59-69) sequence otherwise encoded by exon 9 in goat α(s1) B(2) reference. The same exon skipping event had been identified since 1990, as responsible of the 'weak quantitative class' of α(s1) -CN D variant (0.6 g/L), while the new α(s1) -CN D(1,) has been 'quantitatively' classified as an 'intermediate' variant, since 1.8 g/L per allele was assessed in the milk.
authors.affiliation: Università Federico II Napoli - DSA, Napoli, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Garro
authors.forename: Pasquale
authors.initials: P
authors.lastname: Ferranti
authors.forename: Sabrina
authors.initials: S
authors.lastname: De Pascale
authors.forename: Maria Adalgisa
authors.initials: MA
authors.lastname: Nicolai
authors.forename: Rosalba
authors.initials: R
authors.lastname: Mauriello
authors.forename: Maria
authors.initials: M
authors.lastname: Quarto
authors.forename: Fabio
authors.initials: F
authors.lastname: Pilla
authors.forename: Lina
authors.initials: L
authors.lastname: Chianese
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D015180:Electrophoresis, Gel, Two-Dimensional; D005091:Exons; D006041:Goats / Q000235:genetics*; D015151:Immunoblotting; D013058:Mass Spectrometry / Q000379:methods; D011110:Polymorphism, Genetic; D040901:Proteomics
chemical_list: D002364:Caseins