title: Purification, crystallization and preliminary crystallographic analysis of banyan peroxidase.
abstract: Plant peroxidases are extensively used in a wide range of biotechnological applications owing to their high environmental and thermal stability. A new peroxidase, named banyan peroxidase, was purified from the latex of Ficus benghalensis and crystallized. X-ray diffraction data were collected from native crystals and from bromide and xenon derivatives to resolutions of up to 1.66 Å in the trigonal space group P3(2)21, with unit-cell parameters a = b = 73.1, c = 164.6 Å. The anomalous signal of the intrinsic iron and calcium ions was sufficient for structure solution by SAD, although the sequence is not yet known.
authors.affiliation: Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India.
authors.forename: Anurag
authors.initials: A
authors.lastname: Sharma
authors.forename: Gottfried J
authors.initials: GJ
authors.lastname: Palm
authors.forename: Moni
authors.initials: M
authors.lastname: Kumari
authors.forename: Santosh
authors.initials: S
authors.lastname: Panjikar
authors.forename: M V
authors.initials: MV
authors.lastname: Jagannadham
authors.forename: Winfried
authors.initials: W
authors.lastname: Hinrichs
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D030681:Ficus / Q000201:enzymology*; D008958:Models, Molecular; D010544:Peroxidases / Q000737:chemistry* / Q000302:isolation & purification; D017434:Protein Structure, Tertiary
chemical_list: D010544:Peroxidases