title: Detection of substantial porcine group B rotavirus genetic diversity in the United States, resulting in a modified classification proposal for G genotypes.
abstract: Rotavirus (RV) is an important cause of gastrointestinal disease in animals and humans. In this study, we developed an RT-PCR to detect RV group B (RVB) and characterized the VP7 (G) gene segment detected in porcine samples. One hundred seventy three samples were tested for RV group A (RVA), RVB, and C (RVC) by RT-PCR and examined for RV-like lesion using histopathology. A majority (86.4%) of the samples had mixed RV infections and co-infections of RVA/RVB/RVC were detected at a higher rate (24.3%) than previously reported. RVB was identified in 46.8% of the 173 samples. An adapted VP7 classification was developed using previously published (n=57) and newly sequenced (n=68) RVB strains, resulting in 20 G genotypes based on an 80% nucleotide identity cutoff value. Our results revealed a broad genetic diversity of porcine RVB strains, suggesting RVB has been the cause of common/pre-existing, yet undiagnosed, disease in pigs.
authors.affiliation: University of Minnesota Veterinary Diagnostic Laboratory College of Veterinary Medicine 1333 Gortner Ave Saint Paul, MN 55108, United States. marth027@umn.edu
authors.forename: Douglas
authors.initials: D
authors.lastname: Marthaler
authors.forename: Kurt
authors.initials: K
authors.lastname: Rossow
authors.forename: Marie
authors.initials: M
authors.lastname: Gramer
authors.forename: James
authors.initials: J
authors.lastname: Collins
authors.forename: Sagar
authors.initials: S
authors.lastname: Goyal
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsunemitsu
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Kuga
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki
authors.forename: Max
authors.initials: M
authors.lastname: Ciarlet
authors.forename: Jelle
authors.initials: J
authors.lastname: Matthijnssens
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000145:classification / Q000235:genetics*; D036022:Capsid Proteins / Q000145:classification / Q000235:genetics*; D060085:Coinfection; D003967:Diarrhea / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007421:Intestine, Small / Q000473:pathology / Q000821:virology; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000473:pathology / Q000821:virology*; D014481:United States
chemical_list: D000956:Antigens, Viral; D036022:Capsid Proteins; C050779:VP7 protein, Rotavirus