title: Effect of tilorone and its analogues on the change of mitochondrial potential of rat hepatocytes.
abstract: The influence of tilorone dihydrochloride and its analogues--diphenyl derivatives on the changes of transmembrane potential of mitochondrial membranes of the isolated rat hepatocytes has been estimated. Authors have shown a significant increase in mitochondrial potential thirty minutes after the introduction of the test compounds to the cells using the fluorescent probe JC-1. These results indicate the rapid activation with tilorone and its analog--dihydrochloryde 4,4'-bis-[2-(diethylamino)ethoxy]diphenyl--of the RLR signaling pathway. The final stage of this pathway is the cell production of IFN type I. The authors concluded that there is an increasing of the organelles resistance to the extra/intracellular damaging agents under the influence of the test compounds.
authors.affiliation: D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine. n.zholobak@gmail.com
authors.forename: N M
authors.initials: NM
authors.lastname: Zholobak
authors.forename: N S
authors.initials: NS
authors.lastname: Kavok
authors.forename: O S
authors.initials: OS
authors.lastname: Bogorad-Kobelska
authors.forename: I A
authors.initials: IA
authors.lastname: Borovoy
authors.forename: M Yu
authors.initials: MY
authors.lastname: Malyukina
authors.forename: M Ya
authors.initials: MY
authors.lastname: Spivak
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001562:Benzimidazoles; D002232:Carbocyanines; D002470:Cell Survival / Q000187:drug effects; D005456:Fluorescent Dyes; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007371:Interferon-gamma / Q000096:biosynthesis; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013994:Tilorone / Q000031:analogs & derivatives / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D001562:Benzimidazoles; D002232:Carbocyanines; D005456:Fluorescent Dyes; C068624:5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine; D007371:Interferon-gamma; D013994:Tilorone