title: Molecular cloning and characterization of porcine indoleamine 2, 3-dioxygenase and its expression in various tissues.
abstract: In order to confirm the existence of indoleamine 2, 3-dioxygenase (IDO) gene in swine, and to clone the novel gene followed by the molecule structure properties and expression pattern analysis, the porcine mRNA sequences homologous to human IDO were obtained from GenBank database by bioinformatics method. By using RT-PCR, the IDO gene was cloned from porcine endothelial cell line and the accuracy of the nucleic acid sequence was confirmed, and the expression pattern of the gene was detected. The three-dimensional structure model of porcine IDO was built referring to the tertiary structure of human IDO using biological sequence analysis software and database. The results showed that the porcine IDO was identified by sequencing. The nucleotide sequences were confirmed as a novel gene after submitted to Genbank. Porcine IDO was expressed in the lung, thymus, epididymis and anterior chamber with a basic level, however in peripheral blood mononuclear cells (PBMCs) the IDO gene was highly expressed. The three-dimensional structure model of porcine IDO was similar to that of human IDO. It was suggested that identification of the structure information of porcine IDO is essential to further investigate the immunologic function of the gene. Study of IDO on NK cells-mediated xenograft rejection will be a novel therapeutic target for the development of xenotransplantation.
authors.affiliation: Department of Anesthesiology, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Pediatric Surgery, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Mingfa
authors.initials: M
authors.lastname: Wei
authors.affiliation: Institute of Organ Transplantation, Huazhong University of Science and Technology, Wuhan, 430030, China.|Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan, 430030, China.|Key Laboratory of Organ Transplantation, Ministry of Health, Wuhan, 430030, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Organ Transplantation, Huazhong University of Science and Technology, Wuhan, 430030, China.|Key Laboratory of Organ Transplantation, Ministry of Education, Wuhan, 430030, China.|Key Laboratory of Organ Transplantation, Ministry of Health, Wuhan, 430030, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Department of General Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
authors.forename: Xiangning
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of General Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. minzhutj@yahoo.com.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D042783:Endothelial Cells / Q000378:metabolism; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013552:Swine
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase