title: Laser-engineered topography: correlation between structure dimensions and cell control.
abstract: Topographical cues have a significant impact on cell responses and by this means, on the fabrication of innovative implant materials. However, analysis of cell-topography interactions in dependence of the surface feature dimensions is still challenging due to limitations in the fabrication technology. Here, we introduce surface structuring via picosecond laser systems, which enable a fast production of micro-sized topologies. Changes in the processing parameters further control the feature sizes of so-called spikes. Using surfaces with big and small spike-to-spike-distances for comparisons, we focussed on cell adhesion via extracellular matrix adsorption and focal adhesion complexes, morphology, localisation and proliferation of fibroblasts. The observed cell control was dependent on a turnover point related to the structure dimensions: only big spike-to-spike-distances reduced cell behaviour. Therefore, this technology offers a platform to study cell and tissue interactions with a defined microenvironment.
authors.affiliation: Laser Zentrum Hannover eV, Hannover, Germany. s.schlie@lzh.de
authors.forename: Sabrina
authors.initials: S
authors.lastname: Schlie
authors.forename: Elena
authors.initials: E
authors.lastname: Fadeeva
authors.forename: Anastasia
authors.initials: A
authors.lastname: Koroleva
authors.forename: Boris N
authors.initials: BN
authors.lastname: Chichkov
mesh_terms: D000327:Adsorption; D002448:Cell Adhesion; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D007834:Lasers; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D054457:Tissue Scaffolds
chemical_list: D005353:Fibronectins