title: Determination of selected biogenic amines in red wines by automated on-line combination of capillary isotachophoresis-capillary zone electrophoresis.
abstract: A screening analytical method based on an automated on-line combination of capillary isotachophoresis-capillary zone electrophoresis (cITP-CZE) in hydrodynamically closed separation system, equipped with photometric detection at 280 nm, was developed for a routine determination of the selected biogenic amines, namely histamine, 2-phenylethylamine and tyramine, in red wines. The evaluated limits of detection (LODs) were 0.35 mg L(-1) for histamine, 0.33 mg L(-1) for 2-phenylethylamine and 0.37 mg L(-1) for tyramine. The repeatability of the migration time and peak area for histamine were 1.1% and 2.6%, respectively, for 2-phenylethylamine 0.7% and 2.0%, respectively, and for tyramine 0.8% and 2.1%, respectively. The method recoveries were 92.1% for histamine, 96.4% for 2-phenylethylamine and 95.5% for tyramine. The developed automated cITP-CZE-UV method was applied for the determination of histamine, 2-phenylethylamine and tyramine in seven red wine samples originating from Czech Republic.
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, CZ-77146 Olomouc, Czech Republic. ginterovapavlina@seznam.cz
authors.forename: Pavlína
authors.initials: P
authors.lastname: Ginterová
authors.forename: Jozef
authors.initials: J
authors.lastname: Marák
authors.forename: Andrea
authors.initials: A
authors.lastname: Staňová
authors.forename: Vítězslav
authors.initials: V
authors.lastname: Maier
authors.forename: Juraj
authors.initials: J
authors.lastname: Sevčík
authors.forename: Dušan
authors.initials: D
authors.lastname: Kaniansky
mesh_terms: D001679:Biogenic Amines / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D057625:Isotachophoresis / Q000379:methods*; D057230:Limit of Detection; D015203:Reproducibility of Results; D014920:Wine / Q000032:analysis*
chemical_list: D001679:Biogenic Amines