title: Toll-like receptor 4 signaling promotes the immunosuppressive cytokine production of human cervical cancer.
abstract: OBJECTIVE
  To investigate the expression of TLR-4 (toll-like receptor) on human cervical cancer and find the biological function of the TLR-4 signal system.
  
  
  METHODS
  The immunohistochemistry method was performed to study the protein expression and distribution of TLR-4. The viability of HeLa cells was determined by cell viability assay. Cell proliferation was detected by FCM, ELISA and Western blot were used to observe the gene and protein expression of IL-6 and TGF-beta1 in Hela cell lines.
  
  
  RESULTS
  TLR-4 was over-expressed in cervix cancer, and its activation by LPS promotes proliferation and anti-apoptosis in Hela cells in vitro. Moreover the cell line proliferation increased in a dose- and time-dependent manner. The production of IL-6 and TGF-beta1 were promoted through the activation of the NF-kappaB signaling pathway.
authors.affiliation: Department of Gynaecology and Obstetrics, Renming Hospital of Wuhan University, Hubei, Wuhan, China.
authors.forename: Y X
authors.initials: YX
authors.lastname: Cheng
authors.forename: X Y
authors.initials: XY
authors.lastname: Qi
authors.forename: J L
authors.initials: JL
authors.lastname: Huang
authors.forename: M
authors.initials: M
authors.lastname: Hu
authors.forename: L M
authors.initials: LM
authors.lastname: Zhou
authors.forename: B S
authors.initials: BS
authors.lastname: Li
authors.forename: X X
authors.initials: XX
authors.lastname: Xu
mesh_terms: D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000187:drug effects; D016190:Carboplatin / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006367:HeLa Cells; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000378:metabolism*
chemical_list: D000199:Actins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D051197:Toll-Like Receptor 4; D053773:Transforming Growth Factor beta1; D016190:Carboplatin