title: Delivery of recombinant alphavirus into hippocampal slice tissue culture.
abstract: The alphaviruses Semliki Forest virus (SFV) and Sindbis virus (SIN) have been used frequently as expression vectors in vitro and in vivo. Usually, these systems consist of replication-deficient vectors that require a helper vector for packaging of recombinant particles. Replication-proficient vectors have also been engineered. Alphaviral vectors can be used as nucleic-acid-based vectors (DNA and RNA) or infectious particles. High-titer viral production is achieved in <2 d. The broad host range of alphaviruses facilitates studies in mammalian and nonmammalian cell lines, primary cells in culture, and in vivo. The strong preference for expression in neuronal cells has made alphaviruses particularly useful in neurobiological studies. Unfortunately, their strong cytotoxic effect on host cells, relatively short-term transient expression patterns, and the reasonably high cost of viral production remain drawbacks. However, novel mutant alphaviruses have shown reduced cytotoxicity and prolonged expression. This protocol describes gene delivery of recombinant alphavirus to hippocampal slice cultures. Organotypic slices are covered by a layer of glial cells that impedes the penetration of viral particles to the neurons. Thus, viral particles should be injected manually into the extracellular space of the tissue.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Lundstrom
mesh_terms: D000818:Animals; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006624:Hippocampus / Q000821:virology*; D008845:Microinjections / Q000379:methods*; D009924:Organ Culture Techniques / Q000379:methods; D051381:Rats; D012672:Semliki forest virus / Q000235:genetics* / Q000254:growth & development; D012845:Sindbis Virus / Q000235:genetics* / Q000254:growth & development; D014161:Transduction, Genetic / Q000379:methods