title: Pseudorabies virus and herpes simplex virus type 1 utilize different tegument-glycoprotein interactions to mediate the process of envelopment.
abstract: OBJECTIVE
  During herpesvirus envelopment capsids, tegument polypeptides and membrane proteins assemble at the site of budding, and a cellular lipid bilayer becomes refashioned into a spherical envelope. A web of interactions between tegument proteins and the cytoplasmic tails of viral glycoproteins play a critical role in this process. We have previously demonstrated that for herpes simplex virus (HSV)-1 the cytoplasmic tail of glycoprotein H (gH) binds the tegument protein VP16. The HSV and pseudorabies virus (PRV) genomes are essentially collinear, and individual gene products show significant sequence homology. However, the demarcation of function often differs between PRV and HSV proteins. The goal of this study was to determine whether PRV gH and VP16 interact in a manner similar to their homologs in HSV.
  
  
  METHODS
  A fusion protein pull-down assay was performed in which a PRV gH cytoplasmic tail-glutathione S-transferase fusion protein, bound to glutathione-Sepharose beads, was incubated with PRV-infected cell cytosol, washed and subjected to Western blot analysis using anti-PRV VP16 antisera.
  
  
  RESULTS
  Western blots indicate that PRV VP16 does not specifically bind to the PRV gH tail.
  
  
  CONCLUSIONS
  Our results highlight that, despite the relatively close evolutionary relationship between HSV and PRV, there are significant differences in their protein interactions that drive envelopment.
authors.affiliation: Department of Biology, Farmingdale State College, Farmingdale, N.Y., USA.
authors.forename: Omar S
authors.initials: OS
authors.lastname: Omar
authors.forename: Alicia J
authors.initials: AJ
authors.lastname: Simmons
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Andre
authors.forename: Duncan W
authors.initials: DW
authors.lastname: Wilson
authors.forename: Sarah T
authors.initials: ST
authors.lastname: Gross
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D018259:Herpesvirus 1, Human / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D011558:Herpesvirus 1, Suid / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D025941:Protein Interaction Mapping; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D015678:Viral Structural Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*
chemical_list: D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; C042966:pseudorabies virus glycoproteins