title: [Depot-specific expression of caveolin-1 in human adipose tissue and their relationship with obesity and insulin resistance].
abstract: OBJECTIVE
  To explore the depot-specific mRNA and protein expression of caveolin-1 (CAV-1) in human subcutaneous and omental adipose tissues and analyze their relationship with obesity and insulin resistance.
  
  
  METHODS
  A total of 41 subjects with normal glucose regulation were recruited. Among them, there were 29 cases with normal body mass index (BMI) and 12 cases with BMI ≥ 24 kg/m(2). All were scheduled to undergone selective abdominal operations. The levels of fasting insulin (FINS) were measured by chemiluminescence enzyme-linked immunosorbent assay (ELISA) kit. Fasting plasma glucose (FPG) was tested by glucose oxidase and home model insulin resistance index (HOMA-IR) calculated. Real-time polymerase chain reaction (RT-PCR) and Western blotting were employed to assess the relative mRNA and protein expression of caveolin-1 in subcutaneous and omental adipose tissues. And the mRNA and protein expression of caveolin-1 from different fat depots were compared and their correlations with BMI and HOMA-IR analyzed.
  
  
  RESULTS
  (1) FINS and HOMA-IR were significantly higher in the overweight and obesity group than those in the normal BMI group (FINS: (8.82 ± 3.79) mU/L vs (6.43 ± 4.38) mU/L, P < 0.05, HOMA-1R: 1.91 ± 0.85 vs 1.36 ± 0.72, P < 0.05). (2) The normal BMI group patients had the higher expression levels of caveolin-1 mRNA in omental adipose tissue than overweight counterparts (2.84 ± 0.86 vs 1.02 ± 0.36, P < 0.01). But the difference in subcutaneous adipose tissue was not significant (P > 0.05). (3) The caveolin-1 protein expression in omental adipose tissue of the normal BMI group was higher than that of overweight patients (1.68 ± 0.67 vs 0.73 ± 0.29, P < 0.05). And the difference between two groups was not significant (P > 0.05). (4)The expressions of caveolin-1 mRNA in omental adipose tissue were negatively correlated with BMI, waist circumstance, triglyceride and HOMA-IR (r = -0.441, -0.615, -0.539, -0.688, P < 0.05). No correlations were found between the expressions of caveolin-1 mRNA in subcutaneous adipose tissue with BMI, waist circumstance and HOMA-IR (P > 0.05).
  
  
  CONCLUSIONS
  Differential depot-specific expressions of caveolin-1 are present in human subcutaneous and omental adipose tissues. A low expression of caveolin-1 in omental adipose tissue may contribute to the pathogenesis of obesity and insulin resistance.
authors.affiliation: Department of Endocrinology & Metabolism, Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Li
authors.forename: Hong-yun
authors.initials: HY
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Ya-wei
authors.initials: YW
authors.lastname: Qiu
authors.forename: Long-yi
authors.initials: LY
authors.lastname: Zeng
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D015992:Body Mass Index; D016022:Case-Control Studies; D051242:Caveolin 1 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D050177:Overweight / Q000378:metabolism; D050151:Subcutaneous Fat / Q000378:metabolism; D055815:Young Adult
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1