title: Maintenance of storage properties of pediatric aliquots of apheresis platelets in fluoroethylene propylene containers.
abstract: BACKGROUND
  Platelet (PLT) aliquots for pediatric use have been shown to retain in vitro properties when stored in gas-impermeable syringes for up to 6 hours. As an alternative, PLT aliquots can be stored for longer periods in containers used for storage of whole blood-derived PLTs. These containers are not available separate from whole blood collection sets and PLT volumes less than 35 mL either have not been evaluated or may be unsuitable for PLT storage. Gas-permeable fluoroethylene propylene (FEP) containers have been used in the storage of cell therapy preparations and are available in multiple sizes as single containers but have not been evaluated for PLT storage.
  
  
  METHODS
  A single apheresis unit was divided on Day 3 into small aliquots with volume ranging from 20 to 60 mL, transferred using a sterile connection device, and stored for an additional 2 days either in CLX (control) or in FEP containers. PLT storage properties of PLTs stored in FEP containers were compared to those stored in CLX containers. Standard PLT in vitro assays were performed (n =6).
  
  
  RESULTS
  PLT storage properties were either similar to those of CLX containers or differed by less than 20% excepting carbon dioxide levels, which varied less than 60%.
  
  
  CONCLUSIONS
  Pediatric PLT aliquots of 20, 30, and 60mL transferred on Day 3 into FEP cell culture containers adequately maintain PLT properties for an additional 2days of storage.
authors.affiliation: American Red Cross Biomedical Services, Holland Laboratory, Rockville, Maryland 20855, USA. skripcha@usa.redcross.org
authors.forename: Andrey
authors.initials: A
authors.lastname: Skripchenko
authors.forename: Andrew
authors.initials: A
authors.lastname: Myrup
authors.forename: Dedeene
authors.initials: D
authors.lastname: Thompson-Montgomery
authors.forename: Helen
authors.initials: H
authors.lastname: Awatefe
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Wagner
mesh_terms: D000704:Analysis of Variance; D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D016014:Linear Models; D010372:Pediatrics; D010983:Plateletpheresis; D011138:Polytetrafluoroethylene; D019064:Product Packaging
chemical_list: D011138:Polytetrafluoroethylene