title: Bioactive derivatives of curcumin attenuate cataract formation in vitro.
abstract: In this study, curcumin derivatives salicylidenecurcumin (CD1) and benzalidenecurcumin (CD2)] were prepared, and their biological activity was compared in in vitro selenite-induced cataract model. The antioxidant activity was studied using DPPH radical scavenging assay. Knoevenagel condensates of curcumin exhibited higher DPPH radical scavenging activity compared with curcumin. The anticataractogenic potential of curcumin derivatives was analyzed using lens organ culture method. The activity of antioxidant enzymes and calcium homeostasis was reversed to near normal levels following treatment in organ cultured rat lenses. These results indicated that curcumin and its derivatives--CD1 and CD2--are beneficial against selenite-induced cataract in vitro. Of these, CD1 is having higher bioactive potential compared with curcumin and CD2.
authors.affiliation: Department of Biochemistry, University of Kerala, Kariavattom Campus, Thiruvananthapuram 695 581, Kerala, India.
authors.forename: Asha
authors.initials: A
authors.lastname: Radha
authors.forename: Sudha
authors.initials: S
authors.lastname: Devi Rukhmini
authors.forename: Vilasini
authors.initials: V
authors.lastname: Sasikala
authors.forename: Priya Rajan
authors.initials: PR
authors.lastname: Sakunthala
authors.forename: Balachandran
authors.initials: B
authors.lastname: Sreedharan
authors.forename: Mohanan Puzhavoorparambil
authors.initials: MP
authors.lastname: Velayudhan
authors.forename: Annie
authors.initials: A
authors.lastname: Abraham
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D001597:Benzylidene Compounds / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002386:Cataract / Q000139:chemically induced / Q000188:drug therapy; D002478:Cells, Cultured; D003474:Curcumin / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001597:Benzylidene Compounds; D017382:Reactive Oxygen Species; C579329:benzalidenecurcumin; C579328:salicylidenecurcumin; D013482:Superoxide Dismutase; D000252:Calcium-Transporting ATPases; D003474:Curcumin; D002118:Calcium