title: [Chromogenic in situ hybridization in diagnostics of Epstein-Barr-virus infection in chronic tonsillitis].
abstract: In the issue we demonstrate results of Epstein-Barr virus (EBV) detection by chromogenic in situ hybridization (CISH) in epithelial and lymphoid cells of the palatine tonsil in patients with chronic tonsillitis. Virus genome detections were performed using RNA-probes with digoxigenin-labeled oligonucleotides which target EBV RNA, notably RNA-transcripts of virus genomic DNA. The obtained data confirm the virus lymphotropism and also tropism to epithelial cells of both surface and cryptal epithelium of the palatine tonsil. CISH method in combination with immunohistochemical identification of virus protein products opens new possibilities for clinicopathological monitoring of the different clinical forms of the chronic tonsillitis, as well as new horizon for understanding intrinsic role of EBV in tonsillar pathology.
authors.forename: V P
authors.initials: VP
authors.lastname: Bykova
authors.forename: F A
authors.initials: FA
authors.lastname: Khafizova
authors.forename: D V
authors.initials: DV
authors.lastname: Kalinina
authors.forename: N A
authors.initials: NA
authors.lastname: Daĭkhes
mesh_terms: D002908:Chronic Disease; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D014069:Tonsillitis / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000821:virology
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral