title: [Study on the relationship between altered expression of annexin A4 and endometrial receptivity during the implantation window in infertile patients with endometriosis].
abstract: OBJECTIVE
  To identify the differential expressed proteins, and to investigate the relationship between altered expression of annexin A4 during window of implantation [WOI (at day-6 after ovulatory day)] in infertile patients with endometriosis and endometrial receptivity.
  
  
  METHODS
  Two-dimensional fluorescence differential in-gel electrophoresis (2D-DIGE) and matrix-assist laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) were used to detect protein expression in endometrial WOI in 10 infertile cases with endometriosis as endometriosis group and 10 infertile cases with tubal factors as control group. The semi-quantitative validation of annexin A4 in the eutopic endometrial tissue during WOI was analyzed by western blot.
  
  
  RESULTS
  By comparing protein profiles, there were 7 meaningful differential proteins during WOI in infertile patients with endometriosis. One protein with an isoelectric point of 5.84 and relative molecular weight of 36 100 were down regulated 348% in samples of endometriosis group. It was identified as annexin A4 by mass spectrometry. By western blot, relative intensity of annexin A4 in endometriosis group was 7.2 ± 0.9, which was lower than 17.8 ± 2.6 in control group significantly (t = 7.654, P = 0.002).
  
  
  CONCLUSIONS
  Lower expression of annexin A4 during WOI in infertile patients with endometriosis might be associated with the decrease of endometrial receptivity.
authors.affiliation: Department of Obstetrics and Gynecology, Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, China.
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Jiang
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Fu-qi
authors.initials: FQ
authors.lastname: Xing
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Jian-huai
authors.initials: JH
authors.lastname: Feng
mesh_terms: D000328:Adult; D017314:Annexin A4 / Q000378:metabolism*; D015153:Blotting, Western; D015536:Down-Regulation; D010064:Embryo Implantation / Q000502:physiology*; D004715:Endometriosis / Q000150:complications / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods; D055815:Young Adult
chemical_list: D017314:Annexin A4; D011506:Proteins