title: Nitric oxide-activated calcium/calmodulin-dependent protein kinase regulates the abscisic acid-induced antioxidant defence in maize.
abstract: Nitric oxide (NO), hydrogen peroxide (H2O2), and calcium (Ca2+)/calmodulin (CaM) are all required for abscisic acid (ABA)-induced antioxidant defence. Ca2+/CaM-dependent protein kinase (CCaMK) is a strong candidate for the decoder of Ca2+ signals. However, whether CCaMK is involved in ABA-induced antioxidant defence is unknown. The results of the present study show that exogenous and endogenous ABA induced increases in the activity of ZmCCaMK and the expression of ZmCCaMK in leaves of maize. Subcellular localization analysis showed that ZmCCaMK is located in the nucleus, the cytoplasm, and the plasma membrane. The transient expression of ZmCCaMK and the RNA interference (RNAi) silencing of ZmCCaMK analysis in maize protoplasts revealed that ZmCCaMK is required for ABA-induced antioxidant defence. Moreover, treatment with the NO donor sodium nitroprusside (SNP) induced the activation of ZmCCaMK and the expression of ZmCCaMK. Pre-treatments with an NO scavenger and inhibitor blocked the ABA-induced increases in the activity and the transcript level of ZmCCaMK. Conversely, RNAi silencing of ZmCCaMK in maize protoplasts did not affect the ABA-induced NO production, which was further confirmed using a mutant of OsCCaMK, the homologous gene of ZmCCaMK in rice. Moreover, H2O2 was also required for the ABA activation of ZmCCaMK, and pre-treatments with an NO scavenger and inhibitor inhibited the H2O2-induced increase in the activity of ZmCCaMK. Taken together, the data clearly suggest that ZmCCaMK is required for ABA-induced antioxidant defence, and H2O2-dependent NO production plays an important role in the ABA-induced activation of ZmCCaMK.
authors.affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Ma
authors.forename: Rui
authors.initials: R
authors.lastname: Lu
authors.forename: Huiying
authors.initials: H
authors.lastname: Liu
authors.forename: Ben
authors.initials: B
authors.lastname: Shi
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.forename: Mingpu
authors.initials: M
authors.lastname: Tan
authors.forename: Aying
authors.initials: A
authors.lastname: Zhang
authors.forename: Mingyi
authors.initials: M
authors.lastname: Jiang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000975:Antioxidants / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000648:ultrastructure; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D015854:Up-Regulation / Q000187:drug effects; D003313:Zea mays / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000648:ultrastructure
chemical_list: D000975:Antioxidants; D002147:Calmodulin; D010937:Plant Growth Regulators; D010940:Plant Proteins; D009569:Nitric Oxide; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D002118:Calcium