title: Protective effect of whey protein hydrolysates against hydrogen peroxide-induced oxidative stress on PC12 cells.
abstract: The protective effect of whey protein hydrolysates (WPHs) against H(2)O(2)-induced oxidative damage on rat pheochromocytoma line 12 (PC12) cells was studied. Whey protein was hydrolyzed by pepsin and trypsin and purified by macrospore absorption resins. PC12 cells were pretreated with WPHs (from 369 to 1,980 Da) at different concentrations for 2 h, then washed and incubated with 100 μM H(2)O(2) in the presence of WPHs for another 24 h. With 100-400 μg WPH/ml the viable cells increased by 20-30 % when incubated with H(2)O(2) suggesting that they may play a role as antioxidant in foods.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu, China.
authors.forename: Qiu-Xiang
authors.initials: QX
authors.lastname: Zhang
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Ling
authors.forename: Zhen
authors.initials: Z
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Hui-Xin
authors.initials: HX
authors.lastname: Yu
authors.forename: Samuel Mburu
authors.initials: SM
authors.lastname: Kamau
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: Lu
mesh_terms: D000596:Amino Acids / Q000032:analysis / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008894:Milk Proteins / Q000737:chemistry / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D000067816:Whey Proteins
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D008894:Milk Proteins; D011492:Protein Hydrolysates; D000067816:Whey Proteins; D006861:Hydrogen Peroxide