title: Human trigeminal ganglionic explants as a model to study alphaherpesvirus reactivation.
abstract: Varicella zoster virus (VZV) latency is characterized by limited virus gene expression and the absence of virus DNA replication. Investigations of VZV latency and reactivation have been hindered by the lack of an in vitro model of virus latency. Since VZV is an exclusively human pathogen, we used naturally infected human trigeminal ganglia (TG) obtained at autopsy to study virus latency. Herein, we report optimization of medium to maintain TG integrity as determined by histology and immunohistochemistry. Using the optimized culture medium, we also found that both herpes simplex virus-1 (HSV-1) and VZV DNA replicated in TG explants after 5 days in culture. The increase in HSV-1 DNA was fourfold greater than the increase in VZV DNA. Overall, we present a model for alphaherpesvirus latency in human neurons in which the key molecular events leading to virus reactivation can be studied.
authors.affiliation: Department of Neurology, University of Colorado Denver Medical School, Aurora, CO, USA.
authors.forename: Yevgeniy
authors.initials: Y
authors.lastname: Azarkh
authors.forename: Nathan
authors.initials: N
authors.lastname: Bos
authors.forename: Don
authors.initials: D
authors.lastname: Gilden
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Cohrs
mesh_terms: D001344:Autopsy; D003470:Culture Media; D004279:DNA, Viral / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology*; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008954:Models, Biological; D016133:Polymerase Chain Reaction; D046509:Tissue Culture Techniques; D012668:Trigeminal Ganglion / Q000648:ultrastructure* / Q000821:virology*; D017735:Virus Latency; D014779:Virus Replication
chemical_list: D003470:Culture Media; D004279:DNA, Viral