title: [Effects of electroacupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia].
abstract: OBJECTIVE
  To investigate the effects of electroacupuncture (EA) at Neiguan (PC6) and Xinshu (BL15) on the nerve electrical activity in spinal dorsal root and norepinephrine (NE) and dopamine (DA) concentrations in the paraventricular nucleus of the hypothalamus in rats with acute myocardial ischemia (AMI).
  
  
  METHODS
  A total of 100 male Sprague-Dawley rats were randomly divided into sham-operation, model, EA at PC6, EA at BL15 and EA at both PC6 and BL15 groups with 20 rats in each group. The nerve electrical activity in spinal dorsal roots was recorded by bipolar electrodes. NE and DA concentrations in the paraventricular nucleus of the hypothalamus were detected by high-performance liquid chromatography.
  
  
  RESULTS
  When compared with the sham-operation group, the nerve electrical activity in spinal dorsal roots was significantly increased while the NE and DA concentrations in the paraventricular nucleus of the hypothalamus were decreased in the model group (P<0.01). The nerve electrical activity in spinal dorsal roots was decreased and the NE and DA concentrations were increased in the paraventricular nucleus of the hypothalamus in the EA at PC6 group, the EA at BL15 group and the EA at both PC6 and BL15 group in comparison to those in the model group (P<0.01). The nerve electrical activity in spinal dorsal roots and the NE and DA concentrations in paraventricular nucleus of hypothalamus of the EA at both PC6 and BL15 group were significantly improved when compared to those of the EA at PC6 and EA at BL15 groups (P<0.05).
  
  
  CONCLUSIONS
  EA at both PC6 and BL15 acupoints exhibits the synergistic protective effects against AMI. The possible mechanism is related to regulating the nerve electrical activity in spinal dorsal roots and the concentrations of NE and DA in paraventricular nucleus of the hypothalamus.
authors.affiliation: Institute of Acupuncture, Moxibustion, and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Hu
authors.forename: Rong-lin
authors.initials: RL
authors.lastname: Cai
authors.forename: Zi-jian
authors.initials: ZJ
authors.lastname: Wu
authors.forename: Ke-ming
authors.initials: KM
authors.lastname: Wang
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D004195:Disease Models, Animal; D004298:Dopamine / Q000032:analysis*; D015671:Electroacupuncture; D008297:Male; D017202:Myocardial Ischemia / Q000503:physiopathology*; D009638:Norepinephrine / Q000032:analysis*; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013126:Spinal Nerve Roots / Q000503:physiopathology*
chemical_list: D004298:Dopamine; D009638:Norepinephrine