title: Preparation and anticoagulant activity of N-succinyl chitosan sulfates.
abstract: In order to develop a promising substitute for heparin, N-succinyl chitosan (NSC) was chemically modified by sulfating agent N(SO(3)Na)(3), which were synthesized with sodium bisulfite and sodium nitrite in aqueous solution. The N-succinyl chitosan sulfates (NSCS) products were characterized by infrared spectroscopy (FT-IR) and (13)C NMR. The degree of substitution (DS) of NSCS depended on the ratio of sulfating agent to N-succinyl chitosan, reaction temperature, reaction time and pH of sulfation agent. N-succinyl chitosan sulfates with DS of 1.97 were obtained under optimal conditions. The in vitro coagulation assay of NSCS was determined by activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT) assays. The results showed that NSCS obviously prolonged APTT. The anticoagulant activity strongly depended on DS, molecular weight (M(w)) and concentration of NSCS. The anticoagulant activity of NSCS promoted with the increase of DS and concentration, and NSCS exhibited the best anticoagulant activity with the M(w) of 1.37×10(4).
authors.affiliation: Chemical Engineering Department, Wuhan University of Technology, Wuhan 430070, China.
authors.forename: Tan
authors.initials: T
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.forename: Weiguo
authors.initials: W
authors.lastname: Xie
authors.forename: Lingyun
authors.initials: L
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Zheng
authors.forename: Lihong
authors.initials: L
authors.lastname: Fan
mesh_terms: D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D001780:Blood Coagulation Tests; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013431:Sulfates / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D000925:Anticoagulants; D013431:Sulfates; C480355:chitosan sulfate; D048271:Chitosan