title: Optimization of the URA-blaster disruption system in Candida guilliermondii: efficient gene targeting using the URA3 marker.
abstract: We established a simple transformation system for C. guilliermondii by developing both an ura3 ATCC 6260-derived recipient strain as well as an URA3 blaster cassette. We demonstrated that this strategy allows efficient multiple gene disruption by homologous recombination with a convenient gene targeting frequency.
authors.affiliation: EA2106, Biomolécules et Biotechnologies Végétales, UFR Pharmacie, Université François Rabelais de Tours, France.
authors.forename: Emilien
authors.initials: E
authors.lastname: Foureau
authors.forename: Vincent
authors.initials: V
authors.lastname: Courdavault
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Simkin
authors.forename: Olivier
authors.initials: O
authors.lastname: Pichon
authors.forename: Joël
authors.initials: J
authors.lastname: Crèche
authors.forename: Nathalie
authors.initials: N
authors.lastname: Giglioli-Guivarc'h
authors.forename: Marc
authors.initials: M
authors.lastname: Clastre
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
mesh_terms: D002175:Candida / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D011995:Recombination, Genetic; D012641:Selection, Genetic
chemical_list: D005656:Fungal Proteins