title: Cloning, expression, purification and preliminary X-ray analysis of a putative metagenome-derived lipase.
abstract: LipS is a novel thermostable putative lipase that was isolated from a metagenomic library using functional screening methods. The corresponding gene shows high similarity to that encoding a putative but uncharacterized esterase from Symbiobacterium thermophilum IAM14863 (99% nucleotide-sequence similarity). Two different constructs of the recombinant lipase were crystallized. Crystals belonging to space group P4(2)2(1)2 diffracted X-ray radiation to 2.8 Å resolution and crystals belonging to space group P4 diffracted to 2.0 Å resolution. The most probable content of their asymmetric units were two molecules (P4(2)2(1)2) and four or five molecules (P4), respectively.
authors.affiliation: EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, 22603 Hamburg, Germany.
authors.forename: Francesco
authors.initials: F
authors.lastname: Fersini
authors.forename: Yuliya
authors.initials: Y
authors.lastname: Dall'Antonia
authors.forename: Jennifer
authors.initials: J
authors.lastname: Chow
authors.forename: Wolfgang R
authors.initials: WR
authors.lastname: Streit
authors.forename: Jochen
authors.initials: J
authors.lastname: Mueller-Dieckmann
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D015870:Gene Expression; D008049:Lipase / Q000737:chemistry* / Q000235:genetics; D054892:Metagenome; D008969:Molecular Sequence Data; D016415:Sequence Alignment
chemical_list: D008049:Lipase