title: A simple assay for 6-aminohexanoate-oligomer-hydrolase using N-(4-nitrophenyl)-6-aminohexanamide.
abstract: We synthesized N-(4-nitrophenyl)-6-aminohexanamide (AHpNA) and used it as a substrate in a kinetic study of 6-aminohexanoate-hydrolase (NylB), a nylon oligomer-hydrolyzing enzyme. NylBs derived from Arthrobacter sp. KI72 and Pseudomonas sp. NK87 hydrolyzed AHpNA as well as a 6-aminohexanoic acid dimer, a known substrate for NylB. The K(m) values of the NylB from Arthrobacter sp. KI72 and Pseudomonas sp. NK87 for AHpNA were 0.5 mM and 2.0 mM, respectively.
authors.affiliation: Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan. taguchi@bio.sojo-u.ac.jp
authors.forename: Hisataka
authors.initials: H
authors.lastname: Taguchi
authors.forename: Makoto
authors.initials: M
authors.lastname: Wakamatsu
authors.forename: Kenji
authors.initials: K
authors.lastname: Aso
authors.forename: Shin
authors.initials: S
authors.lastname: Ono
authors.forename: Takashi
authors.initials: T
authors.lastname: Shin
authors.forename: Takashi
authors.initials: T
authors.lastname: Akamatsu
mesh_terms: D000581:Amidohydrolases / Q000032:analysis / Q000378:metabolism*; D000614:Aminocaproates; D015119:Aminocaproic Acid / Q000737:chemistry / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology; D057075:Enzyme Assays / Q000379:methods*; D006868:Hydrolysis; D007700:Kinetics; D009596:Nitrophenols / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: C026243:6-aminohexanoic acid cyclic dimer; D000614:Aminocaproates; C583409:N-(4-nitrophenyl)-6-aminohexanamide; D009596:Nitrophenols; D000581:Amidohydrolases; C026250:6-aminohexanoate-dimer hydrolase; D015119:Aminocaproic Acid