title: Effects of a Mikania laevigata extract on bone resorption and RANKL expression during experimental periodontitis in rats.
abstract: OBJECTIVE
  The Mikania laevigata extract (MLE) (popularly known in Brazil as "guaco") possesses anti-inflammatory properties. In the present study we tested the effects of MLE in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying such effects.
  
  
  METHODS
  Periodontal disease was induced by a ligature placed around the mandibular first molars of each animal. Male Wistar rats were divided into 4 groups: non-ligated animals treated with vehicle; non-ligated animals treated with MLE (10 mg/kg, daily); ligature-induced animals treated with vehicle and ligature-induced animals treated with MLE (10 mg/kg, daily). Thirty days after the induction of periodontal disease, the animals were euthanized and mandibles and gingival tissues removed for further analysis.
  
  
  RESULTS
  Morphometric analysis of alveolar bone loss demonstrated that MLE-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-κB ligand (RANKL) measured by immunohistochemistry. Moreover, gingival tissues from the MLE-treated group showed decreased neutrophil migration myeloperoxidase (MPO) assay.
  
  
  CONCLUSIONS
  These results indicate that MLE may be useful to control bone resorption during progression of experimental periodontitis in rats.
authors.affiliation: Laboratory of Biopathology and Molecular Biology, University of Uberaba, Uberaba, MG, Brazil. brunobbenatti@yahoo.com.br
authors.forename: Bruno B
authors.initials: BB
authors.lastname: Benatti
authors.forename: Jozafá C
authors.initials: JC
authors.lastname: Campos-Júnior
authors.forename: Vilmar J
authors.initials: VJ
authors.lastname: Silva-Filho
authors.forename: Polyanna M
authors.initials: PM
authors.lastname: Alves
authors.forename: Isabela R
authors.initials: IR
authors.lastname: Rodrigues
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Uber-Bucek
authors.forename: Silvio M
authors.initials: SM
authors.lastname: Vieira
authors.forename: Marcelo H
authors.initials: MH
authors.lastname: Napimoga
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001862:Bone Resorption / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D018450:Disease Progression; D008297:Male; D035742:Mikania / Q000737:chemistry*; D010518:Periodontitis / Q000188:drug therapy* / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D018515:Plant Leaves; D053245:RANK Ligand / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D053245:RANK Ligand