title: A comparative study on oxidative and antioxidative markers of serum and follicular fluid in GnRH agonist and antagonist cycles.
abstract: OBJECTIVE
  To determine whether concentrations of oxidative stress markers of follicular fluid and serum are different in GnRH agonist protocol from GnRH antagonist protocol.
  
  
  METHODS
  This was a cross-sectional study. Eighty-four women undergoing controlled ovarian stimulation with either GnRH agonist (n = 39) or GnRH antagonist protocols (n = 45) for IVF/ICSI treatment were assigned by a physician. Blood was obtained at the time of oocyte retrieval, and follicular fluid (FF) from the mature follicles of each ovary was centrifuged and frozen until analysis. Malondialdehyde (MDA), nitric oxide (NO), protein carbonyl (PC), hydroxyl proline (OH-P), sodium oxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GSH-Px), adenosine deaminase (ADA) and xanthine oxidase (XO) were assessed in the serum and follicular fluid of each participants.
  
  
  RESULTS
  The mean serum concentrations of GSH-Px, GSH and MDA were lower in the GnRH antagonist group compared to GnRH agonist group, but mean serum SOD was higher in the GnRH antagonist group. The mean follicular SOD, ADA and NO were higher in GnRH antagonist group than GnRH agonist group. The IVF/ICSI outcomes were similar in both groups.
  
  
  CONCLUSIONS
  GnRH antagonist protocol is associated with increased oxidative stress. The relation of GnRH analogues with oxidative stress and its implication in follicular growth needs to be addressed in further studies.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Inonu University, 44280, Malatya, Turkey. ecelik05@googlemail.com
authors.forename: Ebru
authors.initials: E
authors.lastname: Celik
authors.forename: Onder
authors.initials: O
authors.lastname: Celik
authors.forename: Banu
authors.initials: B
authors.lastname: Kumbak
authors.forename: Ercan
authors.initials: E
authors.lastname: Yilmaz
authors.forename: Ilgin
authors.initials: I
authors.lastname: Turkcuoglu
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Simsek
authors.forename: Abdullah
authors.initials: A
authors.lastname: Karaer
authors.forename: Yagmur
authors.initials: Y
authors.lastname: Minareci
authors.forename: Elif
authors.initials: E
authors.lastname: Ozerol
authors.forename: Kevser
authors.initials: K
authors.lastname: Tanbek
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000737:chemistry; D007987:Gonadotropin-Releasing Hormone / Q000819:agonists* / Q000037:antagonists & inhibitors*; D006727:Hormone Antagonists / Q000008:administration & dosage; D006801:Humans; D007246:Infertility / Q000628:therapy*; D008297:Male; D054315:Oocyte Retrieval; D010062:Ovulation Induction / Q000379:methods; D018384:Oxidative Stress; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D015415:Biomarkers; D006727:Hormone Antagonists; D007987:Gonadotropin-Releasing Hormone