title: [Establishment and characterization of lung adenocarcinoma cell line XLA-07].
abstract: OBJECTIVE
  To establish and characterize a lung adenocarcinoma cell line from a female patient in Xuanwei, Yunnan province.
  
  
  METHODS
  Surgical specimen of the lung adenocarcinoma was obtained and cultured immediately in RPMI 1640 medium with 10% fetal bovine serum and 10(5) U/L penicillin and 100 mg/L streptomycin. When stable proliferation of the cells was achieved after over 40 passages in culture, the biological features of the cell line were investigated by cell morphology, karyotyping, protein marker expression [cytokeratins (CKs), epithelial membrane antigen (EMA) and CD proteins], growth kinetics, cell cycle phase distribution, mitotic index, colony formation in soft agar, cell invasion and tumorigenicity in Balb/c nude mice.
  
  
  RESULTS
  The established cell line was stably cultured for over 80 passages during a one-year period as an anchorage-dependent monolayer of short spindle, polygonal to epithelioid cells under phase contrast microscope. Microglandular cavities and disordered microfilaments were observed under transmission electron microscope. The growth curve presented in an "S" shape with the cell population doubled every 46.7 hours. The mitotic index was 1.5% and the colony formation rate was 8.3%. The cell cycle distribution included 76.9% in G(0)/G(1), 15.1% in S and 8.0% in G(2)/M. The cell line displayed a hypotriploid karyotype with a mode of 66 chromosomes and a median of 64 chromosomes. The cells expressed CK7, CK8, CK (Pan) and EMA by immunohistochemistry. A high level of cell surface expression of CD13 and CD59 was evident by flow cytometry. The cells were able to penetrate Matrigel in vitro but failed to form a stable xenograft in nude mice.
  
  
  CONCLUSIONS
  A new human lung adenocarcinoma cell line, designated as XLA-07, is successfully established from a Xuanwei lung cancer patient.
authors.affiliation: First Affiliated Hospital of Kunming Medical College, Kunming 650032, China.
authors.forename: Li-ju
authors.initials: LJ
authors.lastname: Ma
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Bian
authors.forename: Wen-ping
authors.initials: WP
authors.lastname: Shao
authors.forename: Rui-zhu
authors.initials: RZ
authors.lastname: Tang
authors.forename: Qin-qin
authors.initials: QQ
authors.lastname: Wang
authors.forename: Ke-wei
authors.initials: KW
authors.lastname: Jin
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D000818:Animals; D018826:CD13 Antigens / Q000378:metabolism; D018957:CD59 Antigens / Q000378:metabolism; D018929:Cell Culture Techniques; D002453:Cell Cycle; D045744:Cell Line, Tumor / Q000648:ultrastructure; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D007621:Karyotyping; D007633:Keratins / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D018396:Mucin-1 / Q000378:metabolism; D009368:Neoplasm Transplantation; D011123:Polyploidy; D014410:Tumor Stem Cell Assay
chemical_list: D018957:CD59 Antigens; D018396:Mucin-1; D007633:Keratins; D018826:CD13 Antigens