title: Evaluation of novel adjuvant Eimeria profilin complex on intestinal host immune responses against live E. acervulina challenge infection.
abstract: The effects against avian coccidiosis of two novel adjuvants, Quil A/cholesterol/dimethyl dioctadecyl ammonium bromide/Carbopol (QCDC) and QCDC/Bay R1005 (R)/cytosine-phosphate-guanosine (CpG) oligodeoxynucleotides (CpG ODN [T]) (QCDCRT) emulsified with profilin, a conserved Eimeria recombinant protein, were determined in broiler chickens. Chickens were subcutaneously immunized with isotonic saline (control group), profilin (P), profilin emulsified with QCDC (P-Q), or profilin with QCDCRT (P-QR) at 2 and 9 days post-hatch and orally challenged with 1.0 x 10(4) sporulated oocysts of Eimeria acervulina (EA) at 7 days postimmunization. All profilin-immunized groups showed increased body weight gain when compared to the control group, and the P-QR group had significantly higher body weight gain than did those of the P and P-Q groups following EA challenge infection. All groups immunized with profilin showed significantly decreased intestinal lesions compared with the control group, with the P-QR group showing the lowest intestinal lesions among the profilin-treated groups. Finally, the P-QR group showed greater CD4+/CD8+ and TCR1+/TCR2+ splenocytes and higher antiprofilin serum antibody titers compared with the P and P-Q (or both) groups following EA challenge infection. These results further suggest that vaccination of chickens with profilin, in combination with the QCDCRT adjuvant, may provide a novel control strategy against EA infection in commercial flocks.
authors.affiliation: Animal and Natural Resources Institute, Agricultural Research Service-United States Department of Agriculture, Beltsville, MD 20705, USA.
authors.forename: Sung Hyen
authors.initials: SH
authors.lastname: Lee
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: Seung I
authors.initials: SI
authors.lastname: Jang
authors.forename: Kyung Woo
authors.initials: KW
authors.lastname: Lee
authors.forename: Duk Kyung
authors.initials: DK
authors.lastname: Kim
authors.forename: Erik P
authors.initials: EP
authors.lastname: Lillehoj
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Yancey
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Dominowski
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001835:Body Weight; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology* / Q000517:prevention & control / Q000662:veterinary; D004386:Duodenum / Q000276:immunology / Q000473:pathology; D004539:Eimeria / Q000276:immunology; D006017:Glycolipids / Q000008:administration & dosage; D008214:Lymphocytes / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D051304:Profilins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; C408982:CPG-oligonucleotide; D006017:Glycolipids; D009838:Oligodeoxyribonucleotides; D051304:Profilins; D016052:Protozoan Vaccines; D011994:Recombinant Proteins; C073836:Bay-R 1005