title: Effect of culture conditions on growth, lipid content, and fatty acid composition of Aurantiochytrium mangrovei strain BL10.
abstract: This study explored the influence of various culture conditions on the biomass, lipid content, production of docosahexaenoic acid (DHA), and fatty acid composition of Aurantiochytrium mangrovei strain BL10. The variables examined in this study include the species and concentration of salt, the concentrations of the two substrates glucose and yeast extract, the level of dissolved oxygen, the cerulenin treatment, and the stages of BL10 growth. Our results demonstrate that BL10 culture produces maximum biomass when salinity levels are between 0.2 and 3.0%. Decreasing salinity to 0.1% resulted in a considerable decrease in the biomass, lipid content, DHA production, and DHA to palmitic acid (PA) (DHA/PA) ratio, signifying deterioration in the quality of the oil produced. The addition of 0.9% sodium sulfate to replenish salinity from 0.1% to 1.0% successfully recovered biomass, lipid content and DHA production levels; however, this also led to a decrease in DHA/PA ratio. An increase in oxygen and cerulenin levels resulted in a concomitant decrease in the DHA to docosapentaenoic acid (DPA) (DHA/DPA) ratio in BL10 oil. Furthermore, the DHA/DPA and DHA/PA ratios varied considerably before and after the termination of cell division, which occurred around the 24 hour mark. These results could serve as a foundation for elucidating the biochemistry underlying the accumulation of lipids, and a definition of the extrinsic (environmental or nutritional) and intrinsic (cell growth stage) factors that influence lipid quality and the production of DHA by BL10.
authors.affiliation: Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan. cohens.tw@yahoo.com.tw.
authors.forename: Kai-Chuang
authors.initials: KC
authors.lastname: Chaung
authors.forename: Chun-Yao
authors.initials: CY
authors.lastname: Chu
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Su
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Chen