title: Identification and characterization of a DNA binding domain on the adenovirus IVa2 protein.
abstract: The adenovirus IVa2 protein has been implicated as a transcriptional activator of the viral major late promoter (MLP) and a key component in the packaging of the viral genome. IVa2 functions in packaging through its ability to form a complex with the viral L1 52/55kDa protein, which is required for encapsidation. IVa2, alone and in conjunction with another viral protein, the L4 22K protein, binds to the packaging sequence on the viral genome and to specific elements in the promoter. To define the DNA binding domain on IVa2 and determine its contribution to the viral life cycle, we created a mutant protein that lacks a putative helix-turn-helix motif at the extreme C-terminus. Characterization of this mutant protein showed that while MLP activity is relatively unaffected, it is unable to bind to and package DNA.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109, United States.
authors.forename: Joan B
authors.initials: JB
authors.lastname: Christensen
authors.forename: Sean G
authors.initials: SG
authors.lastname: Ewing
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Imperiale
mesh_terms: D000260:Adenoviruses, Human / Q000502:physiology*; D000595:Amino Acid Sequence; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D016679:Genome, Viral; D019077:Helix-Turn-Helix Motifs; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D014771:Virion / Q000502:physiology; D019065:Virus Assembly
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C571219:L4-22K protein, adenovirus; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D014764:Viral Proteins; C056232:iva2 protein, adenovirus