title: [Expression of lymphokines in CD8(+)HLA-DR(+) T lymphocytes of patients with severe aplastic anemia].
abstract: OBJECTIVE
  To examine the expression of lymphokines damaging hematopoietic cells by CD8(+)HLA-DR(+) effector T cells in peripheral blood (PB) of the patients with severe aplastic anemia (SAA) and explore further the heterogeneous immunopathogenesis of SAA.
  
  
  METHODS
  The CD8(+)HLA-DR(+) cells were sorted by immunomagnetic separation from the PB of 24 untreated SAA patients and 23 normal controls. The mRNA expressions of perforin, granzyme B, FasL and tumor necrosis factor β (TNF-β) of sorted cells were analyzed by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The mRNA levels of perforin, granzyme B of CD8(+)HLA-DR(+)T cells in untreated group were higher than those of the controls (0.66 ± 0.25, 0.56 ± 0.26 vs 0.53 ± 0.14, 0.40 ± 0.13, P = 0.042, 0.012). The mRNA level of FasL in CD8(+)HLA-DR(+) T cells of untreated SAA patients was higher than that of the controls (0.77 ± 0.24 vs 0.61 ± 0.16, P = 0.011). The mRNA of TNF-β in CD8(+)HLA-DR(+) T cells of untreated SAA patients was also higher than that of the controls (0.58 ± 0.16 vs 0.46 ± 0.15, P = 0.011).
  
  
  CONCLUSIONS
  CD8(+)HLA-DR(+) T cells may damage hematopoiesis through the actions of perforin, granzyme B, TNF-β and FasL. And it thus contributes to the immunopathogenesis of SAA.
authors.affiliation: Department of Hematology and Oncology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Wen
authors.initials: W
authors.lastname: Qu
authors.forename: Er-Bao
authors.initials: EB
authors.lastname: Ruan
authors.forename: Hua-Quan
authors.initials: HQ
authors.lastname: Wang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Guo-Jin
authors.initials: GJ
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Wu
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Xing
authors.forename: Jing
authors.initials: J
authors.lastname: Guan
authors.forename: Hong-Lei
authors.initials: HL
authors.lastname: Wang
authors.forename: Tian
authors.initials: T
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Zong-Hong
authors.initials: ZH
authors.lastname: Shao
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D053804:Granzymes / Q000378:metabolism; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D008222:Lymphokines / Q000378:metabolism*; D008233:Lymphotoxin-alpha / Q000378:metabolism; D008297:Male; D054353:Perforin / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D055815:Young Adult
chemical_list: D006684:HLA-DR Antigens; D008222:Lymphokines; D008233:Lymphotoxin-alpha; D012333:RNA, Messenger; D054353:Perforin; D053804:Granzymes