title: [Effect of flavin adenine dinucleotide on ultraviolet B induced damage in cultured human corneal epithelial cells].
abstract: This study evaluated the effects of flavin adenine dinucleotide (FAD) on ultraviolet B (UV-B)-induced damage in cultured human corneal epithelial (HCE-T) cells. The cultured HCE-T cells were treated with 0.003125-0.05% FAD before exposure to 80 mJ/cm2 UV-B. Cell viability was measured 24 h after UV-B irradiation using the MTS assay. Reactive oxygen species (ROS) were detected 30 min after UV-B irradiation using 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate acetyl ester. Apoptosis was evaluated 4 h after UV-B irradiation in the caspase-3/7 activity assay. UV-B irradiation reduced cell viability and stimulated ROS production and caspase-3/7 activity in HCE-T cells. Pretreatment of UV-B irradiated HCE-T cells with FAD significantly attenuated cell viability reduction and inhibited the stimulation of both ROS production and caspase-3/7 activity due to UV-B exposure compared with those with vehicle (0% FAD). These results clarified that FAD inhibits ROS-mediated apoptosis by UV-B irradiation in HCE-T cells and suggest that FAD may be effective as a radical scavenger in UV-B-induced corneal damage.
authors.affiliation: Ophthalmic Research & Development Center, Santen Pharmaceutical Co., Ltd., Ikoma, Nara, Japan. asuka.sakamoto@santen.co.jp
authors.forename: Asuka
authors.initials: A
authors.lastname: Sakamoto
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Nakamura
mesh_terms: D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003315:Cornea / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects; D004847:Epithelial Cells / Q000187:drug effects* / Q000201:enzymology / Q000528:radiation effects; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology*; D006801:Humans; D014466:Ultraviolet Rays
chemical_list: D005182:Flavin-Adenine Dinucleotide; C505407:CASP3 protein, human; C505520:CASP7 protein, human; D053148:Caspase 3; D053179:Caspase 7