title: Mass spectrometry in high-throughput clinical biomarker assays: multiple reaction monitoring.
abstract: Clinical biomarker discovery, verification, and validation are facilitated by the latest technological advances in mass spectrometry. It is now possible to analyze simultaneously group of tens or hundreds of biomarkers in a blood sample using multiple reaction monitoring (MRM), a tandem mass spectrometric method. However, these newly-developed methods face new challenges, including standardization, calibration, and the determination of analytical and biological variation. Here we illustrate the background, pre-analytical sample preparation, and biomarker assay development using an MRM-mass spectrometric method. In addition, special attention is given to future standardization methods to enable widespread use of the technology.
authors.affiliation: University of Victoria - Genome BC Proteomics Centre, University of Victoria, #3101-4464 Markham Street, Vancouver Island Technology Park, Victoria, BC, V8Z 7X8, Canada.
authors.forename: Carol E
authors.initials: CE
authors.lastname: Parker
authors.forename: Dominik
authors.initials: D
authors.lastname: Domanski
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Percy
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Chambers
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Camenzind
authors.forename: Derek S
authors.initials: DS
authors.lastname: Smith
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Borchers
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D003627:Data Interpretation, Statistical; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers