title: [Biophysical methods for biochip analysis. Use of wide field digital fluorescent microscopy].
abstract: This paper discusses an issue on the development of biophysical methods for biochip analysis. A scheme and construction of a biochip analyzer based on wide-field digital fluorescence microscopy are described. The analyzer is designed to register images of biological microchips labeled with fluorescent dyes. The device developed is useful for high-sensitive throughput recording analyses by biochips after interaction of immobilized probes with fluorescently labeled sample molecules as well as it provides the higher rate of the analysis compared to laser scanning devices. With this analyzer a scope where biological microchips can be applied becomes wider, the development of new protocols of the analyses is possible and standard analyses run faster with the use of biochips, the expenses for the analysis performance can be reduced.
authors.forename: V E
authors.initials: VE
authors.lastname: Barskiĭ
authors.forename: E E
authors.initials: EE
authors.lastname: Egorov
authors.forename: É Ia
authors.initials: ÉIa
authors.lastname: Kreĭndlin
authors.forename: Iu P
authors.initials: IuP
authors.lastname: Lysov
authors.forename: S V
authors.initials: SV
authors.lastname: Pan'kov
authors.forename: D A
authors.initials: DA
authors.lastname: Iurasov
authors.forename: R A
authors.initials: RA
authors.lastname: Iurasov
authors.forename: A S
authors.initials: AS
authors.lastname: Zasedatelev
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004867:Equipment Design / Q000295:instrumentation; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D013183:Sputum / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D005456:Fluorescent Dyes