title: Toxicological effects of nanometer titanium dioxide (nano-TiO2) on Chlamydomonas reinhardtii.
abstract: The toxicological effects of nanometer titanium dioxide (nano-TiO2) on a unicellular green alga Chlamydomonas reinhardtii were assessed by investigating the changes of the physiology and cyto-ultrastructure of this species under treatment. We found that nano-TiO2 inhibited photosynthetic efficiency and cell growth, but the content of chlorophyll a content in algae did not change, while carotenoid and chlorophyll b contents increased. Malondialdehyde (MDA) content reached maximum values after 8h exposure and then decreased to a moderately low level at 72 h. Electron microscopy images indicated that as concentrations of nano-TiO2 increased, a large number of C. reinhardtii cells were noted to be damaged: the number of chloroplasts declined, various other organelles were degraded, plasmolysis occurred, and TiO2 nanoparticles were found to be located inside cell wall and membrane. It was also noted that cell surface was surrounded by TiO2 particles, which could present an obstacle to the exchange of substances between the cell and its surrounding environment. To sum up, the effect of nano-TiO2 on C. reinhardtii included cell surface aggregation, photosynthesis inhibition, lipid peroxidation and new protein synthesis, while the response of C. reinhardtii to nano-TiO2 was a rapid process which occurs during 24 h after exposing and may relate to physiological stress system to mitigate damage.
authors.affiliation: State Key Laboratory of Fresh water Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, Hubei 430072, PR China.
authors.forename: Lanzhou
authors.initials: L
authors.lastname: Chen
authors.forename: Lina
authors.initials: L
authors.lastname: Zhou
authors.forename: Yongding
authors.initials: Y
authors.lastname: Liu
authors.forename: Songqiang
authors.initials: S
authors.lastname: Deng
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Gaohong
authors.initials: G
authors.lastname: Wang
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000187:drug effects*; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D002736:Chloroplasts / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000494:pharmacology; D008854:Microscopy, Electron; D053758:Nanoparticles / Q000633:toxicity*; D010788:Photosynthesis / Q000187:drug effects; D014025:Titanium / Q000633:toxicity*
chemical_list: D002734:Chlorophyll; C009495:titanium dioxide; D008315:Malondialdehyde; D014025:Titanium; D000077194:Chlorophyll A