title: Two new palladium(II) complexes: synthesis, characterization and their interaction with HeLa cells.
abstract: Two complexes [Pd(L(1))(en)](2)·4H(2)O (1), [Pd(L(2))(en)]·2H(2)O (2), (L(1) = pyridine-2,3-dicarboxylic acid, L(2) = pyrazine-2,3-dicarboxylic acid, and en = ethylenediamine) have been synthesized and characterized using IR, (1)H NMR and element analysis. X-ray analyses of complexes 1 and 2 revealed that 1 belongs to the triclinic system with space group P1(2), and 2 belongs to the monoclinic system with space group P2(1)/n. The binding of the complexes with HC-DNA (HeLa cells DNA, which was extracted by ourselves) were measured by fluorescence spectrum and viscometry. Gel electrophoresis assay demonstrates the ability of the complexes to cleave the extracted HC-DNA. The complexes exhibit a higher cytotoxicity against tumor cells as against normal cells in vitro. Further more, apoptotic tests indicate that the complexes have an apoptotic effect on HeLa cells.
authors.affiliation: Laboratory of Coordination Chemistry, International Key Laboratory of Shenyang Inorganic Molecule-Based Chemicals, Shenyang University of Chemical Technology, Shenyang, P R China. ejgao@yahoo.com.cn
authors.forename: Enjun
authors.initials: E
authors.lastname: Gao
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Mingchang
authors.initials: M
authors.lastname: Zhu
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
authors.forename: Xianan
authors.initials: X
authors.lastname: Gao
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism; D053837:DNA Cleavage / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008968:Molecular Conformation; D010165:Palladium / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D010165:Palladium; D004247:DNA