title: [Transdifferention of some supporting cells in the cochlea induced by Ad5 atoh1/EGFP in the young adult guinea pigs].
abstract: OBJECTIVE
  To explore whether the Ad5-atoh1/EGFP could transdifferent the supporting cells into the new hair cells in young adult guinea pigs cochlea in vivo.
  
  
  METHODS
  Twelve healthy pigmented guinea pigs weighted 200-250 g were included in this experiment. 5 ul of Ad5-E1/E3 defected-atoh1/EGFP were infused into the scala media through a hole made on the lateral wall of the cochlea. Six of the 12 animal were killed 2 weeks after the infusion operation. The others were killed 4 weeks after the operation. The whole mount of the basal membranes were directly observed under the fluorescence microscope for the expression of the EGFP (enhance green fluorescent protein) or for the expression of the hair cellspecific marker and nuclear after staining with myosin VIIa rabbit polyclonal antibody and Dapi dye.
  
  
  RESULTS
  New cells with big nuclear, ellipse body and expressed with EGFP were found in the region near to the outmost row of the outer hair cells in 2 animal 2 weeks after the infusion. Moreover there were 3 animals with specific morphologic new cells in the location where ever been located by the outer hair cells and the region as 2 weeks animals 4 weeks after the infusion. Those cells were stained by myosin VIIa antibody.
  
  
  CONCLUSIONS
  Atoh1 gene could transdifferent some supporting cells in the basal membrane into hair cell like cells in young adult guinea pigs in vivo. These supporting cells locate in the region of outer hair cells and the basal membrane which do not belong to the region of outer hair cells.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Affiliated Eye Ear Nose Throat Hospital of Fudan University, Shanghai, 200031, China. sfhanzao@yahoo.com.cn
authors.forename: Zhao
authors.initials: Z
authors.lastname: Han
authors.forename: Ning
authors.initials: N
authors.lastname: Cong
authors.forename: Juanmei
authors.initials: J
authors.lastname: Yang
authors.forename: Yibo
authors.initials: Y
authors.lastname: Huang
authors.forename: Kai
authors.initials: K
authors.lastname: Jin
authors.forename: Wen
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D054338:Cell Transdifferentiation / Q000235:genetics*; D003051:Cochlea / Q000166:cytology*; D007758:Ear, Inner; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000166:cytology*; D007760:Labyrinth Supporting Cells / Q000166:cytology*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D049452:Green Fluorescent Proteins; C485184:enhanced green fluorescent protein