title: [Notch pathway gene expression after co-culture of umbilical cord mesenchymal stem cells and hematopoietic stem cells].
abstract: OBJECTIVE
  To detect the gene expression of signal molecules involved in the Notch pathway after co-culture of umbilical cord mesenchymal stem cells (UC-MSCs) and CD34(+); hematopoietic stem cells.
  
  
  METHODS
  UC-MSCs were isolated by collagenase digestion,  and the phenotype was tested by flow cytometry. The differentiation ability of UC-MSCs into adipocytes,  osteoblasts and chondroblasts was analyzed using three induction systems,  respectively. After UC-MSCs were co-cultured in vitro with CD34(+); cells for 6 d,  real-time PCR was applied to investigate the gene expressions of notch ligands (Jagged 1,  2 Delta1,  3,  4),  receptors (Notch1-4) and Hes-1.
  
  
  RESULTS
  The isolated UC-MSCs were found with the typical characteristics of MSCs in morphology,  phenotype and differentiation ability. After  co-culture  in vitro of MSCs and CD34(+); cells,  real-time PCR assay showed a significant up-regulation of Jagged-1 and Notch1. The expression of Hes-1 in CD34(+); cells also  increased,  but there was no obvious change after DAPT (50 nmol/L) was added in co-culture medium.
  
  
  CONCLUSIONS
  Notch signaling may play an important role in the process of the expansion of hematopoietic stem cells supported by UC-MSCs.
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Academy of Military Medical Sciences, Beijing, China. shrui@live.cn
authors.forename: Rui
authors.initials: R
authors.lastname: Shi
authors.forename: Man
authors.initials: M
authors.lastname: Xu
authors.forename: Yong-feng
authors.initials: YF
authors.lastname: Su
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Hu
authors.initials: H
authors.lastname: Chen
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D002135:Calcium-Binding Proteins / Q000235:genetics; D002454:Cell Differentiation; D018920:Coculture Techniques; D005312:Fetal Blood / Q000166:cytology; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D016130:Immunophenotyping; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics; D000072100:Jagged-1 Protein; D008565:Membrane Proteins / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051881:Receptor, Notch1 / Q000235:genetics; D051880:Receptors, Notch / Q000235:genetics* / Q000378:metabolism; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction; D000072056:Transcription Factor HES-1
chemical_list: D018952:Antigens, CD34; D051792:Basic Helix-Loop-Helix Proteins; D002135:Calcium-Binding Proteins; D018398:Homeodomain Proteins; D036341:Intercellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008565:Membrane Proteins; D051881:Receptor, Notch1; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; D000072056:Transcription Factor HES-1; C000606273:JAG1 protein, human; C078493:HES1 protein, human