title: Fluorometric detection of lectin with water-soluble hyperbranched conjugated polymer using mannose mediation.
abstract: A water-soluble hyperbranched polymer containing boronic acid groups at the ends of the polymer, which are capable of binding to diol-containing mannose, was syntheized. The hyperbranched polymer was prepared by a palladium-catalyzed Suzuki cross-coupling reaction using the tribromo monomer for the hyperbranched type structure. The water-soluble hyperbranched polymer (HP) exhibited enhanced fluorescence intensity upon exposure to lectin in the presence of mannose compared to other proteins, such as lysozyme and cytochrome c, because mannose plays a key role in binding both lectin and HP resulting in selective sensing toward lectin.
authors.affiliation: Organic and Optoelectronic Materials Laboratory, Department of Nanotechnology, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Daigeun
authors.initials: D
authors.lastname: Kim
authors.forename: Geun Seok
authors.initials: GS
authors.lastname: Jang
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Son
authors.forename: Seongwon
authors.initials: S
authors.lastname: Seo
authors.forename: Jongho
authors.initials: J
authors.lastname: Kim
authors.forename: Tae Hyeon
authors.initials: TH
authors.lastname: Kim
authors.forename: Taek Seung
authors.initials: TS
authors.lastname: Lee