title: Impact of miR-155 and miR-126 as novel biomarkers on the assessment of disease progression and prognosis in adult T-cell leukemia.
abstract: OBJECTIVE
  Micro RNAs (miRNAs) provide new insight in the development of cancer, but little is known about their clinical relevance as biomarkers in the assessment of diagnosis, classification, progression and prognosis of various cancers. To explore a potential novel biomarker, we examined the cellular and plasma miRNA profiles in adult T-cell leukemia (ATL) characterized by diverse clinical features.
  
  
  RESULTS
  Using CD4-positive cells isolated from 2 non-infected healthy individuals, 3 chronic ATL patients and 3 acute ATL patients, cellular miRNAs were profiled by microarray. The microarray screened 5 miRNAs namely miR-155, let-7g, miR-126, miR-130a and let-7b because of the large difference in their expression in diseased vs. that of healthy controls. The expression levels of before 5 miRNAs re-quantified by reverse transcription quantifiable polymerase chain reaction (RT-qPCR) were not always accordant in cells and plasma. The high and low plasma levels of miR-155 and miR-126 changed with ATL stage.
  
  
  CONCLUSIONS
  The present study revealed that there is a quantitative discrepancy between cellular and plasma miRNAs. The elevation of plasma miR-155 and the reduction in miR-126 correlated with poor prognosis, indicating their usefulness as a novel biomarker for the assessment of disease stage.
authors.affiliation: Central Diagnostic Laboratory of Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan. ishiha@nagasaki-u.ac.jp
authors.forename: Kaori
authors.initials: K
authors.lastname: Ishihara
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sasaki
authors.forename: Kazuto
authors.initials: K
authors.lastname: Tsuruda
authors.forename: Naoko
authors.initials: N
authors.lastname: Inokuchi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nagai
authors.forename: Hiroo
authors.initials: H
authors.lastname: Hasegawa
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.forename: Shimeru
authors.initials: S
authors.lastname: Kamihira
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000097:blood / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; C527111:MIRN126 microRNA, human; C524942:MIRN155 microRNA, human; D035683:MicroRNAs