title: Efficacy of soluble recombinant FliC protein from Salmonella enterica serovar enteritidis as a potential vaccine candidate against homologous challenge in chickens.
abstract: FliC, the flagellin antigen of Salmonella Enteritidis, was tested as a vaccine candidate for protective effect against a homologous challenge in chickens. After immunization with recombinant FliC (rFliC) or administration of phosphate-buffered saline (PBS) at 56 days old, the chickens were challenged with 10(9) colony-forming units of Salmonella Enteritidis at 76 days old. The vaccinated birds showed significantly decreased bacterial counts in the liver and cecal contents compared to those administered PBS at 7 days postchallenge, but the protection was partial. The replication experiment also showed a similar result. In both experiments, vaccination induced an increased level of serum anti-rFliC IgG, which was also reactive to the native flagella. The intestinal IgA level was slightly higher in the vaccinated birds than in the control. However, neither the proliferative response nor interferon-gamma secretion of splenic cells upon stimulation with rFliC was induced. Therefore, the effect of rFliC as a vaccine is limited, and further improvement is needed.
authors.affiliation: Laboratory of Zoonoses, Kitasato University School of Veterinary Medicine, Towada, Aomori 034-8628, Japan. okamuram@vmas.kitasato-u.ac.jp
authors.forename: Masashi
authors.initials: M
authors.lastname: Okamura
authors.forename: Wakako
authors.initials: W
authors.lastname: Matsumoto
authors.forename: Fumio
authors.initials: F
authors.lastname: Seike
authors.forename: Yuuya
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Chie
authors.initials: C
authors.lastname: Teratani
authors.forename: Maki
authors.initials: M
authors.lastname: Tozuka
authors.forename: Takashige
authors.initials: T
authors.lastname: Kashimoto
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Takehara
authors.forename: Masayuki
authors.initials: M
authors.lastname: Nakamura
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshikawa
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000382:microbiology; D005260:Female; D005408:Flagellin / Q000276:immunology; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007273:Injections, Intramuscular / Q000662:veterinary; D008099:Liver / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology*; D013154:Spleen / Q000382:microbiology; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022562:Salmonella Vaccines; D005408:Flagellin