title: [Effect of Fasudil on the phenotype conversion of LPS-stimulated BV-2 microglia].
abstract: OBJECTIVE
  To explore the effect of Fasudil on LPS-stimulated BV-2 microglia in inflammatory reaction and phenotype conversion.
  
  
  METHODS
  The routinely cultured BV-2 microglia in vitro were divided into PBS control group, PBS plus Fasudil treatment group, LPS stimulation group and LPS plus Fasudil group. We determined the production of NO by Griess reaction, the level of TNF-α  by ELISA, and analyzed the M1 and M2 phenotypes of microglia by flow cytometry.
  
  
  RESULTS
  The treatment of LPS lead to the characteristics of M1 phenotype in BV-2 microglia. Fasudil inhibited the production of NO and the release of TNF-α  in LPS-stimulated BV-2 microglia. Interestingly, Fasudil transformed inflammatory M1 cells to anti-inflammatory M2 cells.
  
  
  CONCLUSIONS
  Fasudil shows an anti-inflammatory effect, which may be associated with the conversion of inflammatory M1 microglia to anti-inflammatory M2 cells.
authors.affiliation: Department of Neurology, First Clinical Medical College, Shanxi Medical University, Taiyuan, China. haifei2001@163.com
authors.forename: Hai-fei
authors.initials: HF
authors.lastname: Zhang
authors.forename: Min-fang
authors.initials: MF
authors.lastname: Guo
authors.forename: Jian
authors.initials: J
authors.lastname: Meng
authors.forename: Chun-yun
authors.initials: CY
authors.lastname: Liu
authors.forename: Yan-hua
authors.initials: YH
authors.lastname: Li
authors.forename: Jie-zhong
authors.initials: JZ
authors.lastname: Yu
authors.forename: Shao-wei
authors.initials: SW
authors.lastname: Hou
authors.forename: Ling
authors.initials: L
authors.lastname: Feng
authors.forename: Bao-guo
authors.initials: BG
authors.lastname: Xiao
authors.forename: Cun-gen
authors.initials: CG
authors.lastname: Ma
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D016130:Immunophenotyping; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010641:Phenotype; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C049347:fasudil