title: Phenolic acids in the flowers and leaves of Grindelia robusta Nutt. and Grindelia squarrosa Dun. (Asteraceae).
abstract: 2D-TLC and RP-HPLC methods were applied to qualitatively determinate free phenolic acids and those liberated by acid and alkaline hydrolysis in the flowers and leaves of G. robusta and G. squarrosa. The presence of eleven phenolic acids, namely: caffeic, chlorogenic, p-coumaric, p-hydroxybenzoic, ferulic, gallic, protocatechuic, vanillic salicylic, p-hydroxyphenylacetic and ellagic acids was determined. Quantitative estimate of phenolic acids, expressed as caffeic acid, has been analyzed by the method described in the Polish Pharmacopoeia VIII. The content of phenolic acids in G. robusta reached 7.33 mg/g and 6.23 mg/g for flowers and leaves, respectively. The flowers and leaves of G. squarrosa were characterized by similar level of phenolic acids, namely 6.81 mg/g and 6.59 mg/g, respectively.
authors.affiliation: Department of Pharmacognosy, Medical University of Łódź, 1 Muszyiskiego St., 90-151 Łódź, Poland. slawomira.nowak@umed.lodz.pl
authors.forename: Sławomira
authors.initials: S
authors.lastname: Nowak
authors.forename: Izabela
authors.initials: I
authors.lastname: Rychlińska
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D002855:Chromatography, Thin Layer / Q000592:standards; D035264:Flowers; D036623:Grindelia / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D062385:Hydroxybenzoates / Q000032:analysis*; D018515:Plant Leaves; D012015:Reference Standards
chemical_list: D062385:Hydroxybenzoates; C017616:phenolic acid