title: Effect of yishen huayu fang on kidney tissue E-cadherin expression in unilateral ureter ligation in rats.
abstract: OBJECTIVE
  To observe E-calcium sticky protein (E-cadherin) expression in kidney tissues in a rat model of unilateral ureter ligation and the effect of Yishen Huayu Fang (formula of tonifying the kidney and dissolving accumulated blood stasis) on the expression.
  
  
  METHODS
  A total of 150 clean grade male rats were randomly divided into a control group, model group, low-dose Yishen Huayu Fang group (low-dose group), high-dose Yishen Huayu Fang group (high-dose group), and Lotensin group. A renal fibrosis model was established with unilateral ureteral obstruction (UUO). Pathological changes of rat renal tissue were observed with light microscopy on days 3, 7, 14, 21, and 28 after UUO. Changes in kidney tissue E-cadherin expression were observed with immunohistochemistry.
  
  
  RESULTS
  Three days after modeling, kidney edema appeared followed by gradual inflammatory cell infiltration, and part of the small tubules disappeared while the renal cortex thinned. Meanwhile, the E-cadherin expression level dropped, which was negatively correlated with the obstruction time. After intervention, E-cadherin expression was increased in all treatment groups (P < 0.01 or P < 0.05), while there were no significant differences between the high-dose and Lotensin groups.
  
  
  CONCLUSIONS
  Yishen Huayu Fang delays the renal fibrosis process by promoting E-cadherin expression in renal tissues and reducing extracellular matrix deposition.
authors.affiliation: The First Clinical Medical College of Nanjing University of Traditional Chinese Medicine, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu 210046, China.
authors.forename: Lin-qi
authors.initials: LQ
authors.lastname: Zhang
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
mesh_terms: D000818:Animals; D015820:Cadherins / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal; D005355:Fibrosis; D007150:Immunohistochemistry; D007668:Kidney / Q000737:chemistry* / Q000473:pathology; D008297:Male; D008516:Medicine, Chinese Traditional; D051381:Rats; D017207:Rats, Sprague-Dawley; D014517:Ureteral Obstruction / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D015820:Cadherins; D004365:Drugs, Chinese Herbal; C525740:yishen