title: Evaluation of a microsphere-based immunofluorescence assay for the determination of Immunoglobulin A concentrations in cerebrospinal fluid of dogs.
abstract: The simultaneous increase of immunoglobulin A (IgA) in serum and cerebrospinal fluid (CSF) is a characteristic finding in dogs suffering from canine steroid-responsive meningitis-arteritis (SRMA). The study aimed at developing and evaluating a microsphere-based immunofluorescence assay (MIA) for the measurement of IgA, trying to fulfill the need of a quicker method using only small volumes of CSF. Microsphere beads were coated with goat-anti-dog IgA antibodies and bound IgA was detected by a mouse-anti-dog IgA antibody in combination with a PE-labeled goat-anti-mouse IgG. CSF from 44 dogs were tested for IgA and compared with an in-house utilized ELISA. Using clinical relevant reference ranges, the new method showed a good agreement (84.17%) with the ELISA. A method comparison revealed a moderate agreement only. These findings indicate that the MIA will not replace the ELISA, but it opens the possibility for further research with microsphere-based assays.
authors.affiliation: Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Hannover, Germany. andrea.roerig@tiho-hannover.de
authors.forename: A
authors.initials: A
authors.lastname: Roerig
authors.forename: R
authors.initials: R
authors.lastname: Carlson
authors.forename: A
authors.initials: A
authors.lastname: Tipold
mesh_terms: D000818:Animals; D001167:Arteritis / Q000134:cerebrospinal fluid / Q000276:immunology / Q000662:veterinary; D004283:Dog Diseases / Q000134:cerebrospinal fluid / Q000276:immunology; D004285:Dogs / Q000134:cerebrospinal fluid* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000379:methods / Q000662:veterinary*; D007070:Immunoglobulin A / Q000134:cerebrospinal fluid*; D008581:Meningitis / Q000134:cerebrospinal fluid / Q000276:immunology / Q000662:veterinary; D051379:Mice / Q000276:immunology; D008863:Microspheres
chemical_list: D007070:Immunoglobulin A