title: Molecular cloning and expression analysis of FTZ-F1 in the GIFT tilapia, Oreochromis niloticus.
abstract: The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination. We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia (gfFTZ-F1). The full-length cDNA was isolated from the ovary, which included an open reading frame encoding a predicted protein of 486 amino acids. Sequence, tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues. Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts, though further study is needed to make any definitive conclusions.
authors.affiliation: Shanxi Agricultural University, Shanxi, China. caojinling7928@163.com
authors.forename: Jinling
authors.initials: J
authors.lastname: Cao
authors.forename: Jianjie
authors.initials: J
authors.lastname: Chen
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Jiang
authors.forename: Yongju
authors.initials: Y
authors.lastname: Luo
authors.forename: Xi
authors.initials: X
authors.lastname: Gan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D023681:Cichlids / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005399:Fishes / Q000145:classification / Q000235:genetics; D005786:Gene Expression Regulation; D016366:Open Reading Frames; D057093:Orphan Nuclear Receptors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D029941:Fish Proteins; D057093:Orphan Nuclear Receptors