title: Direct potentiometric determination of diastase activity in honey.
abstract: A novel method for the determination of diastase activity is reported. The method is based on a direct potentiometric measurement of triiodide ion that is released when a starch-triiodide complex is hydrolysed by honey diastase. The increase of free triiodide ion concentration in a sample is found to be directly proportional to the diastase activity of the sample. A response mechanism of the platinum redox electrode is proposed, allowing a calculation of the diastase activity factor (F). The sensor and analyte parameters, including F, were obtained by least squares fitting of potentiometric data using the optimisation function of the Solver add-in of Microsoft Excel. The values of F obtained by the new direct potentiometric method were compared with those obtained using the standard Phadebas method (DN values), and the two values were found to agree within experimental error. Finally, the diastase activity of nine varieties of honey was determined using the novel method developed here.
authors.affiliation: Department of Chemistry, Josip Juraj Strossmayer University of Osijek, F. Kuhača 20, 31000 Osijek, Croatia.
authors.forename: Milan
authors.initials: M
authors.lastname: Sak-Bosnar
authors.forename: Nikola
authors.initials: N
authors.lastname: Sakač
mesh_terms: D000681:Amylases / Q000032:analysis*; D057075:Enzyme Assays; D006722:Honey / Q000032:analysis*; D011199:Potentiometry / Q000379:methods*
chemical_list: D000681:Amylases