title: Role of heat shock protein 27 in gemcitabine-resistant human pancreatic cancer: comparative proteomic analyses.
abstract: The most notable obstacle hindering the effective treatment of human pancreatic             cancer is intrinsic chemoresistance. In order to identify the candidate protein(s)             responsible for the intrinsic chemoresistance, the protein expression profiling             of human pancreatic adenocarcinoma cell line Capan-1 and its distinct surviving             cells following primary treatment with gemcitabine (GEM) were compared by two-dimensional             electrophoresis (2-DE) combined with liquid chromatography-mass spectrometry (LC-MS)             or mass spectrometry (MS). In total, nine proteins were identified, and heat shock             protein B1 (HSP27), one of the differentially expressed proteins, was selected             for further validation. Furthermore, the results of western blotting and immunohistochemical             staining indicated that HSP27 may be significant in pancreatic intrinsic chemoresistance             to GEM. The findings of this study provide a platform for further elucidation             of the underlying mechanisms of pancreatic cancer intrinsic chemoresistance and             demonstrate that HSP27 may be a valid target for anticancer drug development.
authors.affiliation: Department of Pathology, Changhai Hospital, Secondary Military Medical University, Shanghai, PR China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Liu
authors.forename: Chen-Yan
authors.initials: CY
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Can-Yong
authors.initials: CY
authors.lastname: Ni
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Zhu
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D055551:HSP27 Heat-Shock Proteins; D020543:Proteome; D003841:Deoxycytidine; D000093542:Gemcitabine