title: Rapid and sensitive analysis of three polyphenols in tobacco by CE using homemade C(4)D with a mini detection cell.
abstract: A rapid, sensitive, and practical CE with C(4) D detection was developed for the analysis of three polyphenols (rutin, scopoletin, and chlorogenic acid) in tobacco samples. The constructed mini detection cell (12 mm × 10 mm × 10 mm) of C(4) D featured with small inner cell volume (∼2 nL), smaller noise (<0.9 mV), repeatability, high strength and durableness. Three polyphenols were ultrasonically extracted with methanol-water (70:30, v/v) solution following SPE cleanup. The CE method was optimized with the running buffer of 150 mmol L(-1) 2-amino-2-methyl-1-propanol (pH 11.2), and the applied separation voltage of +20 kV over a capillary of 50 μm id × 375 μm od × 50 cm (38 cm to the C(4) D window, 41.5 cm to the UV detector window), which gave a baseline separation of three polyphenols within ca. 6 min. The method provided the limits of quantification (S/N = 10) at about 0.08-0.15 μg g(-1) for three polyphenols, whereas the overall recoveries ranged from 82% to 88%. The proposed method has been successfully applied to measure three polyphenols in the actual tobacco samples, and their contents were calculated and evaluated.
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, P. R. China.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Xie
authors.forename: Yanhao
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.forename: Feifei
authors.initials: F
authors.lastname: Xu
authors.forename: Jianpo
authors.initials: J
authors.lastname: Su
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Lu
authors.forename: Fanya
authors.initials: F
authors.lastname: Zeng
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Yaxiao
authors.initials: Y
authors.lastname: Guo
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhang
mesh_terms: D002726:Chlorogenic Acid / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D012431:Rutin / Q000032:analysis* / Q000737:chemistry; D012603:Scopoletin / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D002726:Chlorogenic Acid; D012431:Rutin; D012603:Scopoletin