title: [Splicing factor SC35 regulated the expression of B7-H3 in vitro].
abstract: OBJECTIVE
  To explore the mechanisms by which splicing factor SC35 regulates the costimulatory molecule B7-H3 expression in vitro through bioinformatic and molecular  biological methods.
  
  
  METHODS
  We screened some regulatory proteins which might take part in regulating B7-H3 expression using bioinformatic methods. Then RNA interference (RNAi) and real-time PCR were performed to test if these proteins took part in the regulation.
  
  
  RESULTS
  Splicing factor SC35, SRP40 and SF50 might play an important role in the regulation of B7-H3 expression. In PHA-activated T cells, B7-H3 was upregulated obviously and at the same time SC35 was also upregulated. When we suppressed SC35 expression using RNAi, we found B7-H3 was also downregulated.
  
  
  CONCLUSIONS
  Splicing factor SC35 might take part in the regulation of B7-H3 expression, which could help understand B7-H3 biological function.
authors.affiliation: Department of Laboratory & Pharmacy, Suzhou Health College, Suzhou, China.
authors.forename: Li-xian
authors.initials: LX
authors.lastname: Yi
authors.forename: Bing
authors.initials: B
authors.lastname: Gao
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Feng-qing
authors.initials: FQ
authors.lastname: Fu
authors.forename: Xue-guang
authors.initials: XG
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
mesh_terms: D060887:B7 Antigens / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism*; D034622:RNA Interference; D016601:RNA-Binding Proteins / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism*; D000068103:Serine-Arginine Splicing Factors; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D060887:B7 Antigens; C423237:CD276 protein, human; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C073288:SRSF2 protein, human; D000068103:Serine-Arginine Splicing Factors