title: Effects of fatty acids on human serum albumin binding centers.
abstract: Albumin is a carrier of nonesterified long-chain fatty acids and many other ligands. The status of its binding centers was studied for various proportions of nonesterified long-chain fatty acids and albumin as exemplified by palmitic acid. The status of the binding center was tested by recording K-35 probe fluorescence decay in the subnanosecond band. This method showed the work of three types of centers. Palmitic acid enhanced binding activity of all centers, though to a different degree: if the palmitic acid/albumin proportion increased to 2-3, the probe binding to type 1 centers (located in the drug center I region) increased 1.5 times, while binding to type 3 centers increased more than 3-fold. Modification of these centers by nonesterified long-chain fatty acids was similar in the isolated human albumin preparation and in diluted blood serum. Hence, K-35 probe showed the actual status of various albumin centers, their binding capacity depending to a different measure on the fatty acid charge of albumin.
authors.affiliation: Institute of Physicochemical Medicine, the Russian Academy of Sciences, Moscow, Russia.
authors.forename: G E
authors.initials: GE
authors.lastname: Dobretsov
authors.forename: T I
authors.initials: TI
authors.lastname: Syrejshchikova
authors.forename: N V
authors.initials: NV
authors.lastname: Smolina
authors.forename: M G
authors.initials: MG
authors.lastname: Uzbekov
mesh_terms: D005227:Fatty Acids / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D006801:Humans; D007094:Imides; D009281:Naphthalenes; D010169:Palmitic Acids / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D012709:Serum Albumin / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D007094:Imides; C112259:N(n-carboxy)phenylimide-4-dimethyl-aminonaphthalic acid; D009281:Naphthalenes; D010169:Palmitic Acids; D012709:Serum Albumin