title: Lack of independent relationship between the MMP-12 gene polymorphism and carotid plaque susceptibility in the Chinese Han population.
abstract: The purpose of this study was to investigate whether a polymorphism in the matrix metalloproteinase-12 gene (MMP-12 -82A/G) is correlated with serum protein levels or with the susceptibility for carotid plaques in the Chinese Han population. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed on the -82A/G polymorphism in the MMP-12 gene for 1314 patients with acute cerebral infarctions; 710 of these cases were diagnosed with stable plaques, 340 cases were diagnosed with vulnerable plaques and 264 cases had no plaques. At the same time, serum MMP-12 levels were measured using the enzyme-linked immunosorbent assay (ELISA). Compared to the AA genotype, the frequency of the AG+GG genotypes was not significantly different between the three groups (χ(2) = 1.242, p = 0.537), and the frequency of the G allele of the MMP-12 gene was not different within the three subgroups (χ(2) = 1.218, p = 0.544). There were no significant differences in MMP-12 protein levels among the three groups (F = 0.675, p = 0.510); similarly, there was no difference in MMP-12 protein levels between the stable plaque group and the vulnerable plaque group (p = 0.755). There was also no difference between the vulnerable plaque group and the no plaque group (p = 0.420). The current data suggest that the inter-individual variability in the MMP-12 gene variation may not be a risk factor for vulnerable plaques in the Chinese Han population.
authors.affiliation: Department of Neurology, Taizhou Hospital, Affiliated Hospital of Wenzhou Medical College, Taizhou, Zhejiang, PR China.
authors.forename: Weiling
authors.initials: W
authors.lastname: Li
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Jin
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.forename: XianFang
authors.initials: X
authors.lastname: Lin
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Hu
authors.forename: Wanfen
authors.initials: W
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Guofei
authors.initials: G
authors.lastname: Jin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D002339:Carotid Arteries / Q000000981:diagnostic imaging / Q000201:enzymology*; D002340:Carotid Artery Diseases / Q000000981:diagnostic imaging / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D002544:Cerebral Infarction / Q000201:enzymology / Q000208:ethnology / Q000235:genetics; D016009:Chi-Square Distribution; D002681:China / Q000453:epidemiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D016015:Logistic Models; D008297:Male; D053508:Matrix Metalloproteinase 12 / Q000235:genetics*; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D058226:Plaque, Atherosclerotic; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011379:Prognosis; D018570:Risk Assessment; D012307:Risk Factors; D012422:Rupture, Spontaneous; D014463:Ultrasonography
chemical_list: C507122:MMP12 protein, human; D053508:Matrix Metalloproteinase 12