title: A versatile and colorful screening tool for the identification of arabinofuranose-acting enzymes.
abstract: Selecting wall-nibblers: Three 4-nitrocatechol derivatives were designed to facilitate high-throughput screening of arabinofuranose hydrolases, enzymes that typically digest plant cell walls. The designed compounds can be used in solid and liquid media, and, importantly, one allows the specific detection of AXH-d, a specialized enzyme that only releases L-arabinose from disubstituted D-xylosyl moieties.
authors.affiliation: Université de Toulouse; INSA, UPS, INP; LISBP, 135 Avenue de Rangueil, Toulouse, France.
authors.forename: Vinciane
authors.initials: V
authors.lastname: Borsenberger
authors.forename: Fernando
authors.initials: F
authors.lastname: Ferreira
authors.forename: Annick
authors.initials: A
authors.lastname: Pollet
authors.forename: Emmie
authors.initials: E
authors.lastname: Dornez
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Desrousseaux
authors.forename: Stéphane
authors.initials: S
authors.lastname: Massou
authors.forename: Christophe M
authors.initials: CM
authors.lastname: Courtin
authors.forename: Michael J
authors.initials: MJ
authors.lastname: O'Donohue
authors.forename: Régis
authors.initials: R
authors.lastname: Fauré
mesh_terms: D001089:Arabinose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002396:Catechols / Q000737:chemistry / Q000378:metabolism*; D003124:Colorimetry / Q000379:methods; D057075:Enzyme Assays / Q000379:methods*; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D006868:Hydrolysis; D009574:Nitro Compounds / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002396:Catechols; D009574:Nitro Compounds; C431714:arabinofuranose; C506350:nitrocatechol; D001089:Arabinose