title: 1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum  2CR371.5.
abstract: 1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing  genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.
authors.affiliation: A&A Biotechnology, Gdynia, Poland. sd@aabiot.com
authors.forename: Sławomir
authors.initials: S
authors.lastname: Dąbrowski
authors.forename: Dorota
authors.initials: D
authors.lastname: Pietrewicz-Kubicz
authors.forename: Ewa
authors.initials: E
authors.lastname: Zabłotna
authors.forename: Anna
authors.initials: A
authors.lastname: Długołęcka
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D046972:Clostridium butyricum / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005822:Genetic Vectors; D005985:Glyceraldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006836:Hydro-Lyases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D011407:Propane / Q000378:metabolism; D011409:Propylene Glycols / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011409:Propylene Glycols; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; C041787:1,3-propanediol; D006836:Hydro-Lyases; C020671:glycerol dehydratase; D005990:Glycerol; D011407:Propane