title: Comparison of stimulating effect on subpopulations of lymphocytes in human peripheral blood by methionine enkephalin with IL-2 and IFN-γ.
abstract: The aim of this study was to investigate the effects of mechanisms of methionine enkephalin (MENK) on lymphocytes in human peripheral blood. We detected CD4+T cells, CD8+T cells, CD4+CD25+ regulatory T cells (Treg), dendritic cells (DCs), natural killer cells (NK), NKT cells and γδT cells before and after treatment with 10 (-12) M MENK, in cell culture by FCM and RT-PCR. Our findings show that MENK stimulating expansion of lymphocyte subpopulationns by inhibiting CD4+CD25+ regulatory T cells (Treg), which is unique discovery of our study. We may use MENK as a drug to treat cancer patients, whose immune systems are damaged by chemotherapy or radiotherapy.
authors.affiliation: Department of Immunology; School of Basic Medical Science; China Medical University, Shenyang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Hua
authors.forename: Changlong
authors.initials: C
authors.lastname: Lu
authors.forename: Weiwei
authors.initials: W
authors.lastname: Li
authors.forename: Jingjuan
authors.initials: J
authors.lastname: Meng
authors.forename: Danan
authors.initials: D
authors.lastname: Wang
authors.forename: Nicolas P
authors.initials: NP
authors.lastname: Plotnikoff
authors.forename: Enhua
authors.initials: E
authors.lastname: Wang
authors.forename: Fengping
authors.initials: F
authors.lastname: Shan
mesh_terms: D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D004744:Enkephalin, Methionine / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007155:Immunologic Factors / Q000378:metabolism*; D007371:Interferon-gamma / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*; D008297:Male; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D007155:Immunologic Factors; D007376:Interleukin-2; D004744:Enkephalin, Methionine; D007371:Interferon-gamma