title: Phytochemical investigations and biological potential screening with cellular and non-cellular models of globe amaranth (Gomphrena globosaL.) inflorescences.
abstract: Gomphrena globosaL. is a popular edible plant used as food colourant and in traditional medicine. In this work, 24 phenolic compounds and eight betacyanins were determined by HPLC-DAD in three different extracts of G. globosa inflorescences. The decoction presented the highest amount of phenolic compounds, kaempferol-3-O-(6-rhamnosyl)hexoside plus kaempferol-3-O-hexoside being the main compounds. The rich betacyanins extract showed isogomphrenin III and gomphrenin III as major metabolites. Decreases in the nitric oxide amounts were observed in LPS-stimulated RAW 264.7 cells. In vitro scavenging micro assays against several reactive species revealed a great antioxidant capacity, particularly against superoxide radical. In general, the best results were obtained with decoction. Some relationships between activity and composition were established. The data provide evidence of the G. globosa inflorescences potential as a source of anti-inflammatory compounds, with relevance for the treatment of acute or chronic inflammatory conditions, and health-promoting antioxidants for use by both food and pharmaceutical industries.
authors.affiliation: REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, n.° 228, 4050-313 Porto, Portugal.
authors.forename: Luís R
authors.initials: LR
authors.lastname: Silva
authors.forename: Patrícia
authors.initials: P
authors.lastname: Valentão
authors.forename: Joana
authors.initials: J
authors.lastname: Faria
authors.forename: Federico
authors.initials: F
authors.lastname: Ferreres
authors.forename: Carla
authors.initials: C
authors.lastname: Sousa
authors.forename: Angel
authors.initials: A
authors.lastname: Gil-Izquierdo
authors.forename: Brígida R
authors.initials: BR
authors.lastname: Pinho
authors.forename: Paula B
authors.initials: PB
authors.lastname: Andrade
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D050859:Betacyanins / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D056626:Inflorescence / Q000737:chemistry*; D051379:Mice; D010636:Phenols / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D050859:Betacyanins; D010636:Phenols; D010936:Plant Extracts