title: Yeasts from glacial ice of Patagonian Andes, Argentina.
abstract: Glacial ice and snow are known habitats for cold-adapted microorganisms. Research on cold-adapted yeast biodiversity from Perito Moreno and Mount Tronador glaciers (Patagonia, Argentina), and production of extracellular enzymatic activity at low temperatures (5 and 18 °C), was performed and described in this study. Ninety percent (90%) of the isolates were basidiomycetous; 16 genera and 29 species were identified. Twenty-five percent (25%) of total isolates corresponded to psychrophilic yeasts, whereas 75% were psychrotolerant yeasts. Eighty-five percent (85%) of all isolates had at least one enzymatic activity. Multiple correspondence analysis and cluster classification revealed a relationship between certain genera and some enzymatic activities. Cold-adapted yeast isolates were able to hydrolyze natural compounds (casein, lipids, starch, pectin, and carboxymethylcellulose) at low temperatures, suggesting a significant ecological role of these organisms as organic matter decomposers and nutrient cyclers. These yeasts are especially relevant for metabolic and ecological studies, as well as for yeast-based biotechnological process at low temperatures.
authors.affiliation: Laboratorio de Microbiología Aplicada y Biotecnología, Centro Regional Universitario Bariloche, Universidad Nacional del Comahue, INIBIOMA (CONICET-UNCo), Río Negro, Argentina. vikidegarcia@gmail.com
authors.forename: Virginia
authors.initials: V
authors.lastname: de Garcia
authors.forename: Silvia
authors.initials: S
authors.lastname: Brizzio
authors.forename: María Rosa
authors.initials: MR
authors.lastname: van Broock
mesh_terms: D001118:Argentina; D044822:Biodiversity; D003080:Cold Temperature; D004271:DNA, Fungal / Q000235:genetics; D046448:Ice Cover / Q000382:microbiology*; D016533:Mycological Typing Techniques; D010802:Phylogeny; D015003:Yeasts / Q000145:classification* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004271:DNA, Fungal