title: Expression of  the bovine papillomavirus type 1, 2 and 4 L1 genes in the yeast Pichia  pastoris.
abstract: Papillomaviruses  are known to cause benign or malignant lesions in various animals. In cattle,  bovine papillomavirus (BPV) is the etiologic agent of papillomatosis and  neoplasia of the upper gastrointestinal tract and urinary bladder. Currently,  there are no standard diagnostic tests or prophylactic vaccines. Protection  against papillomavirus infection is conferred by neutralizing antibodies  directed towards the major structural protein L1. These antibodies can be  efficiently induced by immunization with virus-like particles that are formed  spontaneously after L1 gene expression in recombinant systems. The yeast Pichia  pastoris is known to provide an efficient system for expression of proteins  due to reduced cost and high levels of protein production. We evaluated P.  pastoris for expression of the L1 gene from BPV1, BPV2 and BPV4. After  methanol induction, the recombinants were able to produce L1 proteins of the  three different BPV types. To increase heterologous L1 protein levels, a codon  optimization strategy was used for production under bioreactor conditions. The  BPV1 L1 protein was identified by monoclonal antibody anti-6xHis. This is the  first report of BPV L1 expression in yeast.
authors.affiliation: Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brasil.
authors.forename: A L S
authors.initials: AL
authors.lastname: Jesus
authors.forename: F C
authors.initials: FC
authors.lastname: Mariz
authors.forename: H M
authors.initials: HM
authors.lastname: Souza
authors.forename: M N
authors.initials: MN
authors.lastname: Cordeiro
authors.forename: E C
authors.initials: EC
authors.lastname: Coimbra
authors.forename: M C G
authors.initials: MC
authors.lastname: Leitão
authors.forename: L M
authors.initials: LM
authors.lastname: Nascimento
authors.forename: R C
authors.initials: RC
authors.lastname: Stocco
authors.forename: W
authors.initials: W
authors.lastname: Beçak
authors.forename: A C
authors.initials: AC
authors.lastname: Freitas
mesh_terms: D000818:Animals; D015153:Blotting, Western; D052688:Bovine papillomavirus 1 / Q000235:genetics*; D052697:Bovine papillomavirus 4 / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle; D003062:Codon / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics*; D010843:Pichia / Q000378:metabolism*; D011995:Recombination, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D036022:Capsid Proteins; D003062:Codon; C085217:L1 protein, Bovine papillomavirus