title: Sensing immune responses with customized peptide microarrays.
abstract: The intent to solve biological and biomedical questions in high-throughput led to an immense interest in microarray technologies. Nowadays, DNA microarrays are routinely used to screen for oligonucleotide interactions within a large variety of potential interaction partners. To study interactions on the protein level with the same efficiency, protein and peptide microarrays offer similar advantages, but their production is more demanding. A new technology to produce peptide microarrays with a laser printer provides access to affordable and highly complex peptide microarrays. Such a peptide microarray can contain up to 775 peptide spots per cm², whereby the position of each peptide spot and, thus, the amino acid sequence of the corresponding peptide, is exactly known. Compared to other techniques, such as the SPOT synthesis, more features per cm² at lower costs can be synthesized which paves the way for laser printed peptide microarrays to take on roles as efficient and affordable biomedical sensors. Here, we describe the laser printer-based synthesis of peptide microarrays and focus on an application involving the blood sera of tetanus immunized individuals, indicating the potential of peptide arrays to sense immune responses.
authors.affiliation: Department Chip-based Peptide Libraries, German Cancer Research Center (DKFZ), Heidelberg, Germany. c.schirwitz@dkfz.de
authors.forename: Christopher
authors.initials: C
authors.lastname: Schirwitz
authors.forename: Felix F
authors.initials: FF
authors.lastname: Loeffler
authors.forename: Thomas
authors.initials: T
authors.lastname: Felgenhauer
authors.forename: Volker
authors.initials: V
authors.lastname: Stadler
authors.forename: Frank
authors.initials: F
authors.lastname: Breitling
authors.forename: F Ralf
authors.initials: FR
authors.lastname: Bischoff
mesh_terms: D000328:Adult; D006801:Humans; D056724:Immunity, Humoral; D007158:Immunologic Techniques / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*; D013745:Tetanus Toxoid / Q000008:administration & dosage / Q000276:immunology
chemical_list: D013745:Tetanus Toxoid