title: A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis.
abstract: We designed a protein ladder (hereafter referred to as "Mega-tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R(2)=0.997±0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.
authors.affiliation: Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Chien-Han
authors.initials: CH
authors.lastname: Kao
authors.forename: Chiu-Min
authors.initials: CM
authors.lastname: Cheng
authors.forename: Kuo-Hsiang
authors.initials: KH
authors.lastname: Chuang
authors.forename: Chih-Hung
authors.initials: CH
authors.lastname: Chuang
authors.forename: Shey-Cherng
authors.initials: SC
authors.lastname: Tzou
authors.forename: Ta-Chun
authors.initials: TC
authors.lastname: Cheng
authors.forename: Yuan-Chin
authors.initials: YC
authors.lastname: Hsieh
authors.forename: Kuang-Wen
authors.initials: KW
authors.lastname: Liao
authors.forename: Yun-Ming
authors.initials: YM
authors.lastname: Wang
authors.forename: Long-Sen
authors.initials: LS
authors.lastname: Chang
authors.forename: Steve R
authors.initials: SR
authors.lastname: Roffler
authors.forename: Fang Ming
authors.initials: FM
authors.lastname: Chen
authors.forename: Tian-Lu
authors.initials: TL
authors.lastname: Cheng
mesh_terms: D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D000939:Epitopes; D011993:Recombinant Fusion Proteins