title: [Identification of aberrantly expressed miRNAs in rectal cancer].
abstract: OBJECTIVE
  To identify aberrantly expressed miRNAs in rectal cancer.
  
  
  METHODS
  We used the miRCURY™ Array® LNA microRNA chip (v.14.0) to evaluate miRNA expression levels between rectal cancer tissues and adjacent non-tumor tissues; an average change more than 2-fold (and P value less than 0.05) was set as a cutoff level. All 6 paired rectal cancers were classified pathology stage C or D.
  
  
  RESULTS
  Eighty-eight miRNAs were up-regulated and 46 miRNAs have been reported in colorectal cancer; 40 miRNAs were down-regulated in rectal cancers and 15 miRNAs have been reported in colorectal cancer. To compare the relative miRNA expression levels as measured by RT-qPCR and chip analysis, we analyzed expression levels of these miRNAs in the cancer tissues. The results showed that miRNA expression (increased or decreased) in the paired benign and tumor tissue was consistent between the two methods in all cases. Expression levels of 6 up-regulated miRNAs (by chip analysis compared to RT-qPCR) varied in a range from -11.9% to 39.1% . Expression levels of 5 down-regulated miRNAs varied in a range from 1.4% to 29.4%. The Pearson correlation of relative miRNAs expression levels was analyzed by cDNA array versus RT-qPCR, and found to be 0.96 (P<0.01).
  
  
  CONCLUSIONS
  miRNA profile in rectal cancer showed unique characteristics, and identified a series of new, aberrantly expressed miRNAs.
authors.affiliation: Department of Gastroenterology, Central South University, Changsha, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Li
authors.forename: Guiying
authors.initials: G
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Meihua
authors.initials: M
authors.lastname: Xu
authors.forename: Deyun
authors.initials: D
authors.lastname: Feng
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs