title: Quantitative proton nuclear magnetic resonance evaluation and total assignment of the capsular polysaccharide Neisseria meningitidis serogroup X.
abstract: Neisseria meningitidis constitutes the main cause of meningococcal disease in infants. Serogroups A, B, C, W135, Y, and X have the higher incidence in young children and teenagers. The use of polyvalent conjugate carbohydrate-based vaccines has decreased the meningococcal infection around the world. Recently, the serogroup X has been found to be responsible of different outbreaks of meningococcal diseases, mainly in "Meningitis Belt" of Africa and the structure of the repetitive unit of the capsular polysaccharide has been confirmed through a monodimensional (13)C NMR study. No further characterization studies have been carried out, especially with the use of other nuclei. In this paper a novel method for quantification of the N. meningitidis serogroup X by proton qNMR is reported. Deep characterization of the serogroup X polysaccharide was also carried out by combination of correlation experiments involving (13)C, (1)H, and (31)P nuclei.
authors.affiliation: Center of Biomolecular Chemistry, 200 Street and 21 Ave. Atabey, Playa, La Habana, Cuba. raine.garrido@cqb.cu
authors.forename: Raine
authors.initials: R
authors.lastname: Garrido
authors.forename: Alain
authors.initials: A
authors.lastname: Puyada
authors.forename: Anaís
authors.initials: A
authors.lastname: Fernández
authors.forename: Majela
authors.initials: M
authors.lastname: González
authors.forename: Ubel
authors.initials: U
authors.lastname: Ramírez
authors.forename: Felix
authors.initials: F
authors.lastname: Cardoso
authors.forename: Yury
authors.initials: Y
authors.lastname: Valdés
authors.forename: Domingo
authors.initials: D
authors.lastname: González
authors.forename: Violeta
authors.initials: V
authors.lastname: Fernández
authors.forename: Vicente
authors.initials: V
authors.lastname: Vérez
authors.forename: Hermán
authors.initials: H
authors.lastname: Vélez
mesh_terms: D016667:Bacterial Capsules / Q000737:chemistry* / Q000276:immunology; D002236:Carbohydrate Conformation; D002247:Carbon Isotopes; D016014:Linear Models; D015394:Molecular Structure; D009345:Neisseria meningitidis / Q000737:chemistry* / Q000276:immunology; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010759:Phosphorus Isotopes; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology; D011522:Protons; D015203:Reproducibility of Results
chemical_list: D002247:Carbon Isotopes; D010759:Phosphorus Isotopes; D011135:Polysaccharides, Bacterial; D011522:Protons