title: [Variation of the enzymatic activity of Bothrops atrox "jergon" snake venom from three geographic regions, Peru].
abstract: OBJECTIVE
  To study the variability in the composition and enzymatic activity of venom from adult Bothrops atrox specimens.
  
  
  METHODS
  We used venoms from adult snakes from Amazonas, Junín and Ucayali. Each of the venom samples underwent analysis for protein and number of bands by pagesds. Phospholipase A₂, hemolytic, amidolytic, coagulant, hemorrhagic activity were analyzed, also and proteolytic activity on casein and by zymogram. Additionally, immunodiffusion and neutralization assays in vitro were done with a polyvalent botropic serum from the national institute of health of Peru.
  
  
  RESULTS
  The amidolytic, coagulant, hemorrhagic, proteolytic by zymogram, phospholipase A₂, and indirect hemolytic activity were variable, demonstrating increased activity in the venoms from Amazonas, regarding proteolytic by zymogram, phospholipase A₂, and indirect hemolytic activity. While the amount of protein electrophoretic bands and proteolytic activity on casein did not demonstrated differences. Regarding neutralization tests, a 0.5 dose of antivenom was sufficient to effectively neutralize (>50%) the coagulant activity and phospholipase A₂ of all samples analyzed.
  
  
  CONCLUSIONS
  Some biological properties of the venom from adult Bothrops atrox of Peru are variable, without interference with the in vitro neutralization by the polyvalent botropic serum on coagulant and phospholipase A₂ properties of the venom.
authors.affiliation: Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú. cesar.alexander04@gmail.com
authors.forename: César
authors.initials: C
authors.lastname: Ortiz
authors.forename: Fanny
authors.initials: F
authors.lastname: Lazo
authors.forename: Candy
authors.initials: C
authors.lastname: Bellido
authors.forename: Edgar
authors.initials: E
authors.lastname: Gonzales
authors.forename: Armando
authors.initials: A
authors.lastname: Yarlequé
mesh_terms: D000818:Animals; D017837:Bothrops; D003435:Crotalid Venoms / Q000201:enzymology*; D010568:Peru
chemical_list: D003435:Crotalid Venoms