title: Proteomic analysis of differences in ectoderm and mesoderm membranes by DiGE.
abstract: Ectoderm and mesoderm can be considered as prototypes for epithelial and mesenchymal cell types. These two embryonic tissues display clear differences in adhesive and motility properties, which are phenomenologically well characterized but remain largely unexplored at the molecular level. Because the key downstream regulations must occur at the plasma membrane and in the underlying actin cortical structures, we have set out to compare the protein content of membrane fractions from Xenopus ectoderm and mesoderm tissues using 2-dimensional difference gel electrophoresis (DiGE). We have thus identified several proteins that are enriched in one or the other tissues, including regulators of the cytoskeleton and of cell signaling. This study represents to our knowledge the first attempt to use proteomics specifically targeted to the membrane-cortex compartment of embryonic tissues. The identified components should help unraveling a variety of tissue-specific functions in the embryo.
authors.affiliation: Department of Biology, McGill University, Montreal, Canada.
authors.forename: Renee
authors.initials: R
authors.lastname: Wang
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Liu
authors.forename: Elke
authors.initials: E
authors.lastname: Küster-Schöck
authors.forename: François
authors.initials: F
authors.lastname: Fagotto
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004475:Ectoderm / Q000737:chemistry* / Q000196:embryology; D007150:Immunohistochemistry; D042541:Intracellular Space; D008648:Mesoderm / Q000737:chemistry* / Q000196:embryology; D009928:Organ Specificity; D025341:Principal Component Analysis; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*; D014981:Xenopus
chemical_list: D011506:Proteins; D020543:Proteome