title: The new role of LOX-1 in hypertension induced neuronal apoptosis.
abstract: Lectin-like oxidized low-density lipoprotein (oxLDL) receptor-1 (LOX-1) was originally identified as a receptor for oxLDL predominantly expressed in endothelial cells. Recently up-regulation of LOX-1 has been implicated in oxidative stress and cell apoptosis in many cell types. However, LOX-1 expression in neurons or regulation of neuronal apoptosis by LOX-1 has not been reported. To investigate the possible roles of LOX-1 in hypertension induced brain damage, we examined the distribution of LOX-1 in cortex and hippocampus and compared its expression in 32-week-old SHR and WKY rats. Immunofluorescence revealed that LOX-1 positive cells were located principally at the cortex involved in sensory information processing and were mainly expressed in neurons. We also found up-regulated mRNA expression of LOX-1, Bax and caspase-3 and down-regulated mRNA expression of Bcl-2 in SHR group. Compared with WKY group, SHR group showed increased LOX-1 positive cells and TUNEL positive cells. Furthermore, double-labeling method indicated that LOX-1 expression was colocalized with TUNEL positive cells, which means that LOX-1 expression was involved in hypertension related cell apoptosis. These findings indicated that LOX-1 expression was up-regulated in the cortex of SHR and its expression has implication in neuronal apoptosis. Elevated Bax/Bcl-2 ratio may be involved under this event.
authors.affiliation: Department of Cardiology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Li
authors.forename: Zongming
authors.initials: Z
authors.lastname: Duan
authors.forename: Dengfeng
authors.initials: D
authors.lastname: Gao
authors.forename: Shengli
authors.initials: S
authors.lastname: Huang
authors.forename: Haifeng
authors.initials: H
authors.lastname: Yuan
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Niu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology*; D006973:Hypertension / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D051127:Scavenger Receptors, Class E / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
chemical_list: C495136:OLR1 protein, rat; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger; D051127:Scavenger Receptors, Class E; D051028:bcl-2-Associated X Protein