title: Serum proteomics of methamphetamine addicts and up-regulation of complement factor H related to methamphetamine addiction.
abstract: Methamphetamine (METH) is a new type of drug with strong tolerance and addiction. However, the molecular mechanisms underlying the processes of METH addiction are still not fully understood. To determine possible biomarkers and mechanisms that are responsible for METH addiction, a 2-DE based proteomics approach was used to evaluate the changes in protein expression of the serum in Chinese patients addicted to METH, which to the best of our knowledge is the first study of its kind. We identified five proteins that were markedly altered and complement factor H (CFH), the most stably up-expressed protein in each 2-DE experiment, was further studied using the rat conditioned place preference (CPP) model to detect any changes to its expression in the sera and six brain regions of interest. We report, for the first time, that CFH was positive related to METH addiction.
authors.affiliation: National Key Research Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, PR China.
authors.forename: Wan-Lu
authors.initials: WL
authors.lastname: Shi
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Liu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Bing-Ying
authors.initials: BY
authors.lastname: Zhou
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Pu
mesh_terms: D000328:Adult; D019969:Amphetamine-Related Disorders / Q000097:blood*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001921:Brain / Q000378:metabolism; D017242:Complement Factor H / Q000096:biosynthesis*; D003214:Conditioning, Classical / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D008694:Methamphetamine / Q000008:administration & dosage*; D020543:Proteome / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum; D055815:Young Adult
chemical_list: D015415:Biomarkers; D020543:Proteome; D008694:Methamphetamine; D017242:Complement Factor H