title: Brachial artery flow-mediated dilation following exercise with augmented oscillatory and retrograde shear rate.
abstract: BACKGROUND
  Acute doses of elevated retrograde shear rate (SR) appear to be detrimental to endothelial function in resting humans. However, retrograde shear increases during moderate intensity exercise which also enhances post-exercise endothelial function. Since SR patterns differ with the modality of exercise, it is important to determine if augmented retrograde SR during exercise influences post-exercise endothelial function. This study tested the hypothesis that (1) increased doses of retrograde SR in the brachial artery during lower body supine cycle ergometer exercise would attenuate post-exercise flow-mediated dilation (FMD) in a dose-dependent manner, and (2) antioxidant vitamin C supplementation would prevent the attenuated post-exercise FMD response.
  
  
  METHODS
  Twelve men participated in four randomized exercise sessions (90 W for 20 minutes) on separate days. During three of the sessions, one arm was subjected to increased oscillatory and retrograde SR using three different forearm cuff pressures (20, 40, 60 mmHg) (contralateral arm served as the control) and subjects ingested placebo capsules prior to exercise. A fourth session with 60 mmHg cuff pressure was performed with 1 g of vitamin C ingested prior to the session.
  
  
  RESULTS
  Post-exercise FMD following the placebo conditions were lower in the cuffed arm versus the control arm (arm main effect: P < 0.05) and without differences between cuff pressures (20 mmHg: 5.7 ± 2.2%; 40 mmHg: 4.7 ± 1.3%; 60 mmHg: 5.4 ± 2.4%) (P > 0.05). Following vitamin C treatment, post-exercise FMD in the cuffed and control arm increased from baseline (P < 0.05) but were not different (control: 7.1 ± 3.5% vs. cuffed: 6.6 ± 3.3%) (P > 0.05).
  
  
  CONCLUSIONS
  These results indicate that augmented oscillatory and retrograde SR in non-working limbs during lower body exercise attenuates post-exercise FMD without an evident dose-response in the range of cuff pressures evaluated. Vitamin C supplementation prevented the attenuation of FMD following exercise with augmented oscillatory and retrograde SR suggesting that oxidative stress contributes to the adverse effects of oscillatory and retrograde shear during exercise on FMD.
authors.affiliation: Department of Kinesiology, Indiana University, Bloomington, IN, USA. Johnson.Blair@mayo.edu
authors.forename: Blair D
authors.initials: BD
authors.lastname: Johnson
authors.forename: Kieren J
authors.initials: KJ
authors.lastname: Mather
authors.forename: Sean C
authors.initials: SC
authors.lastname: Newcomer
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Mickleborough
authors.forename: Janet P
authors.initials: JP
authors.lastname: Wallace
mesh_terms: D000328:Adult; D001916:Brachial Artery / Q000000981:diagnostic imaging / Q000502:physiology*; D004311:Double-Blind Method; D004730:Endothelium, Vascular / Q000000981:diagnostic imaging / Q000502:physiology; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D018737:Hand Strength; D006801:Humans; D008297:Male; D012016:Reference Values; D012039:Regional Blood Flow / Q000502:physiology*; D013314:Stress, Mechanical; D018615:Ultrasonography, Doppler, Color / Q000379:methods*; D014664:Vasodilation / Q000502:physiology*