title: LncRNAs expression signatures of renal clear cell carcinoma revealed by microarray.
abstract: BACKGROUND
  Long noncoding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. They are aberrantly expressed in many types of cancers. In this study, we described lncRNAs profiles in 6 pairs of human renal clear cell carcinoma (RCCC) and the corresponding adjacent nontumorous tissues (NT) by microarray.
  
  
  RESULTS
  With abundant and varied probes accounting 33,045 LncRNAs in our microarray, the number of lncRNAs that expressed at a certain level could be detected is 17157. From the data we found there were thousands of lncRNAs that differentially expressed (≥ 2 fold-change) in RCCC tissues compared with NT and 916 lncRNAs differentially expressed in five or more of six RCCC samples. Compared with NT, many lncRNAs were significantly up-regulated or down-regulated in RCCC. Our data showed that down-regulated lncRNAs were more common than up-regulated ones. ENST00000456816, X91348, BC029135, NR_024418 were evaluated by qPCR in sixty-three pairs of RCCC and NT samples. The four lncRNAs were aberrantly expressed in RCCC compared with matched histologically normal renal tissues.
  
  
  CONCLUSIONS
  Our study is the first one to determine genome-wide lncRNAs expression patterns in RCCC by microarray. The results displayed that clusters of lncRNAs were aberrantly expressed in RCCC compared with NT samples, which revealed that lncRNAs differentially expressed in tumor tissues and normal tissues may exert a partial or key role in tumor development. Taken together, this study may provide potential targets for future treatment of RCCC and novel insights into cancer biology.
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Gan
authors.initials: G
authors.lastname: Yu
authors.forename: Weimin
authors.initials: W
authors.lastname: Yao
authors.forename: Ji
authors.initials: J
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Ding
authors.initials: D
authors.lastname: Xia
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Kangli
authors.initials: K
authors.lastname: Deng
authors.forename: Haibing
authors.initials: H
authors.lastname: Xiao
authors.forename: Bohan
authors.initials: B
authors.lastname: Wang
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Guo
authors.forename: Wei
authors.initials: W
authors.lastname: Guan
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Hu
authors.forename: Yinqi
authors.initials: Y
authors.lastname: Bai
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.forename: Jihong
authors.initials: J
authors.lastname: Liu
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhangqun
authors.initials: Z
authors.lastname: Ye
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks / Q000235:genetics; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015854:Up-Regulation / Q000235:genetics
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger