title: The impact of dehydration rate on the production and cellular location of reactive oxygen species in an aquatic moss.
abstract: OBJECTIVE
  The aquatic moss Fontinalis antipyretica requires a slow rate of dehydration to survive a desiccation event. The present work examined whether differences in the dehydration rate resulted in corresponding differences in the production of reactive oxygen species (ROS) and therefore in the amount of cell damage.
  
  
  METHODS
  Intracellular ROS production by the aquatic moss was assessed with confocal laser microscopy and the ROS-specific chemical probe 2,7-dichlorodihydrofluorescein diacetate. The production of hydrogen peroxide was also quantified and its cellular location was assessed.
  
  
  RESULTS
  The rehydration of slowly dried cells was associated with lower ROS production, thereby reducing the amount of cellular damage and increasing cell survival. A high oxygen consumption burst accompanied the initial stages of rehydration, perhaps due to the burst of ROS production.
  
  
  CONCLUSIONS
  A slow dehydration rate may induce cell protection mechanisms that serve to limit ROS production and reduce the oxidative burst, decreasing the number of damaged and dead cells due upon rehydration.
authors.affiliation: Universidade de Lisboa, Departamento de Biologia Vegetal and Center for Biodiversity, Functional & Integrative Genomics (BioFIG), Campo Grande, Lisboa, Portugal.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Cruz de Carvalho
authors.forename: Myriam
authors.initials: M
authors.lastname: Catalá
authors.forename: Jorge
authors.initials: J
authors.lastname: Marques da Silva
authors.forename: Cristina
authors.initials: C
authors.lastname: Branquinho
authors.forename: Eva
authors.initials: E
authors.lastname: Barreno
mesh_terms: D019068:Bryopsida / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D002470:Cell Survival; D003681:Dehydration; D003890:Desiccation; D006861:Hydrogen Peroxide / Q000032:analysis / Q000378:metabolism; D018613:Microscopy, Confocal; D018384:Oxidative Stress / Q000502:physiology; D010100:Oxygen / Q000378:metabolism*; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013997:Time Factors
chemical_list: D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D010100:Oxygen