title: Chemical composition, antimicrobial, antioxidant and cytotoxic activity of the essential oil from the leaves of Acanthopanax leucorrhizus (Oliv.) Harms.
abstract: The leaf essential oil of Acanthopanax leucorrhizus, a widely used medicinal plant, was obtained by hydrodistillation and analyzed by using combination of capillary GC-FID, GC-MS and RI. Fifty-nine components, representing 93.1% of the total oil, were identified in the essential oil and the main components of the oil were β-pinene (7.3%), linalool (6.5%), p-cymene (6.3%), β-elemene (3.8%), γ-terpinene (3.7%), spathulenol (3.2%) and cis-sabinene hydrate (3.1%). Furthermore, the in vitro antimicrobial, antioxidant and cytotoxic activities of the essential oil were examined. The test results showed that the essential oil exhibited a broad spectrum of anti-microbial activity against all microorganisms tested. Gram-positive bacteria were more sensitive to the oil than gram-negative bacteria and yeasts. The oil possessed moderate cytotoxicity on human tumor cells with lower IC(50) values of 25.65μg/ml (Hep G2), 28.71μg/ml (Hela), 30.15μg/ml (Bel-7402) and 37.55μg/ml (A-549). The moderate antioxidant activity of the oil was also evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical method.
authors.affiliation: College of Chemistry and Chemical Engineering, Longdong University, Qingyang 745000, People's Republic of China. Electronic address: hhb-88@126.com.
authors.forename: Haobin
authors.initials: H
authors.lastname: Hu
authors.affiliation: College of Chemistry and Chemical Engineering, Longdong University, Qingyang 745000, People's Republic of China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zheng
authors.affiliation: College of Chemistry and Chemical Engineering, Longdong University, Qingyang 745000, People's Republic of China.
authors.forename: Huaisheng
authors.initials: H
authors.lastname: Hu
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002175:Candida / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003603:Cytotoxins / Q000494:pharmacology*; D028001:Eleutherococcus; D006801:Humans; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves; D012441:Saccharomyces cerevisiae / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D003603:Cytotoxins; D009822:Oils, Volatile