title: Tomography and static-mechanical properties of adherent cells.
abstract: A tomography approach is used to reconstruct 3D cell shapes and, simultaneously, the shapes/positions of the nuclei within these cells. Subjecting the cells to well-defined microconfinements of various diameters allow for relating the steady-state shapes of cells to their static-mechanical properties. The observed shapes show striking regularities between different cell types and all fit to a model that takes into account the cell membrane, cortical actin, and the nucleus.
authors.affiliation: Department of Chemical and Biological Engineering, Northwestern University, Illinois 60208, USA.
authors.forename: Siowling
authors.initials: S
authors.lastname: Soh
authors.forename: Kristiana
authors.initials: K
authors.lastname: Kandere-Grzybowska
authors.forename: Goher
authors.initials: G
authors.lastname: Mahmud
authors.forename: Sabil
authors.initials: S
authors.lastname: Huda
authors.forename: Alexander Z
authors.initials: AZ
authors.lastname: Patashinski
authors.forename: Bartosz A
authors.initials: BA
authors.lastname: Grzybowski
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D048430:Cell Shape; D006224:Cricetinae; D003412:Cricetulus; D051379:Mice; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism; D014054:Tomography
chemical_list: D000199:Actins