title: Clinical and laboratory evidence of Trichomonas vaginalis infection among women of reproductive age in rural area.
abstract: BACKGROUND
  Vaginitis is a commonly encountered complaint and one of the most frequent reasons for patient visit to obstetrician-gynaecologists. Three vaginal infections are frequent causes of a vaginal discharge: (1) bacterial vaginosis, (2) vulvovaginal candidiasis and (3) trichomonas vaginitis. Differences in the clinical presentation are helpful in diagnosis. Characteristic signs and symptoms for these three vaginal infections are distinct, but on many occasions, they are overlapping. The aim of the present study was to find the prevalence and correlation between the clinical spectrum and laboratory evidence of Trichomonas vaginalis infection by simple, reliable, confirmatory and specific method, i.e. microscopic examination of wet mount preparation and acridine stain of vaginal fluid.
  
  
  METHODS
  Irrespective of HIV status, a total of 156 women with vaginal discharge were studied for establishing diagnosis of genital tract infection. The cases of bacterial vaginosis and vulvovaginal candidiasis were excluded from the study. Vaginal speculum assisted high vaginal swabs were collected from women with discharge, during collection vagina was inspected for obvious signs.
  
  
  RESULTS
  Of the 156 women with vaginal discharge, 19 (12.06 %) showed T. vaginalis infection. All the women belonged to active reproductive age group, i.e. 20-40 years. Itching dysuria, and offensive, malodorous, thin, yellowish vaginal discharge were the main and consistent complaints. Only in 2 (1.52%) cases, vaginal speculum examination revealed erythema and punctuate haemorrhage, the so-called "strawberry' vagina. The pH was recorded to be >4.5.
  
  
  CONCLUSIONS
  Clinical differentiation of various forms of infectious vaginitis is unreliable. The prevalence of T. vaginalis infection at 12.06% was found among rural young women of reproductive age using simple and reliable screening wet mount microscopy.
authors.affiliation: Department of Microbiology, Jawaharlal Nehru Medical College, Sawangi, Wardha, Maharashtra, India.
authors.forename: S R
authors.initials: SR
authors.lastname: Fule
authors.forename: R P
authors.initials: RP
authors.lastname: Fule
authors.forename: N S
authors.initials: NS
authors.lastname: Tankhiwale
mesh_terms: D000328:Adult; D019411:Clinical Laboratory Techniques / Q000379:methods; D015510:Clinical Medicine / Q000379:methods; D005260:Female; D006801:Humans; D010274:Parasitology / Q000379:methods; D015995:Prevalence; D012424:Rural Population; D014247:Trichomonas Vaginitis / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology / Q000473:pathology; D014246:Trichomonas vaginalis / Q000302:isolation & purification*; D019522:Vaginal Discharge / Q000453:epidemiology / Q000209:etiology*; D055815:Young Adult