title: Characterization of extended-spectrum beta-lactamase-producing Salmonella enterica serotype Brunei and Heidelberg at the Hussein Dey hospital in Algiers (Algeria).
abstract: The purpose of this work was to study the genetic determinants responsible for extended-spectrum cephalosporin (ESC) resistance of Salmonella collected during the period of 1995-2008 at the Hussein Dey hospital in Algiers (Algeria). Fourteen ESC-resistant Salmonella isolates were tested towards 22 antimicrobial agents. Polymerase chain reaction (PCR) and sequencing were used to determine the underlying genetic determinants responsible for the extended-spectrum beta-lactamase (ESBL) phenotypes. Enterobacterial Repetitive Intergenic Consensus PCR was employed to type the isolates. All tested isolates were resistant to ticarcillin, ticarcillin-clavulanate, piperacillin, cefuroxime, aztreonam, ceftazidime, cefotaxime (except two isolates), cefepime, and cefpirome. PCR and DNA sequencing identified these ESBLs as TEM-48 (n=6), TEM-4 (n=3), CTX-M-15 (n=4), and one new TEM, designated TEM-188. Thus, continued surveillance for the presence of ESBL-producing (non-typhoidal) salmonellae in Algeria is essential.
authors.affiliation: Département de Microbiologie, FSNV, Université A/MIRA de Béjaia, Béjaia, Algeria.
authors.forename: Rachida
authors.initials: R
authors.lastname: Kermas
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Touati
authors.forename: Lucien
authors.initials: L
authors.lastname: Brasme
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Le Magrex-Debar
authors.forename: Sadjia
authors.initials: S
authors.lastname: Mehrane
authors.forename: François-Xavier
authors.initials: FX
authors.lastname: Weill
authors.forename: Christophe
authors.initials: C
authors.lastname: De Champs
mesh_terms: D000462:Algeria; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D018550:Cephalosporin Resistance; D004269:DNA, Bacterial; D003968:Diarrhea, Infantile / Q000188:drug therapy / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial; D005243:Feces / Q000382:microbiology; D005759:Gastroenteritis / Q000188:drug therapy / Q000382:microbiology; D014644:Genetic Variation; D006786:Hospitals, Urban; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D058889:Molecular Typing; D012480:Salmonella Infections / Q000188:drug therapy / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification / Q000201:enzymology* / Q000378:metabolism; D013045:Species Specificity; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D047090:beta-Lactams / Q000378:metabolism* / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D047090:beta-Lactams; D001618:beta-Lactamases