title: Inhibition of hypoxia and serum deprivation-induced apoptosis by salvianolic acid in rat mesenchymal stem cells.
abstract: OBJECTIVE
  To investigate the influence and mechanism of salvianolic acid B (SalB) on apoptosis inhibition in rat bone marrow-derived mesenchymal stem cells (BMSCs) induced by hypoxia and serum deprivation (hypoxia/SD).
  
  
  METHODS
  SalB concentration of 0.1, 1, 10 or 100 mg/L (drug groups) were investigated for their ability to inhibit apoptosis in rat BMSCs. BMSCs in both the apoptosis model and drug groups were cultured under hypoxic conditions for 6 h, after which cell apoptosis and change in mitochondrial membrane potential (MMP) were detected using flow cytometry. Activation of caspase-3 was detected using western blot analysis.
  
  
  RESULTS
  Hypoxia/SD induced apoptosis in rat BMSCs. The early apoptosis rate was lower in the drug groups compared to the apoptosis model group (P < 0.05). SalB was found to inhibit the reduction in MMP and decrease the activation of caspase-3.
  
  
  CONCLUSIONS
  0.1, 1 and 10 mg/L of SalB inhibits activation of caspase-3 and early apoptosis of rat BMSCs induced by hypoxia/SD and could therefore enhance the survival rate of grafted stem cells.
authors.affiliation: Guang'an men Hospital Affiliated to China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Xiao-wen
authors.initials: XW
authors.lastname: Guo
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Rui-xia
authors.initials: RX
authors.lastname: Xu
authors.forename: Hu-zhan
authors.initials: HZ
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001572:Benzofurans / Q000494:pharmacology*; D015687:Cell Hypoxia / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza; D053630:Depsides
chemical_list: D001572:Benzofurans; D016895:Culture Media, Serum-Free; D004365:Drugs, Chinese Herbal; C076944:salvianolic acid B; D053630:Depsides