title: Histochemical  map of the ectopic expression of the Arabidopsis atExt1 extensin gene in  transgenic tobacco.
abstract: Histochemical  analysis of transgenic tobacco plants harboring the promoter of the Arabidopsis extensin gene atExt1 fused to the β-glucuronidase gene coding sequence  demonstrated expression of the transgene in stem tissues. The transgene was  expressed in cells of the internal and external phloem at the nodal and  internodal regions of the older parts of mature stems. In younger parts of the  same stem, expression of the transgene was slightly modified: expression was  detected in the internal phloem in the internodal areas. In the nodal areas,  the phloem tissue and the surrounding parenchyma were stained, demonstrating  transgene expression. Expression was also seen in the phloem of the  inflorescence; it was non-specific at the junctions of the flowers to the  inflorescence. β-glucuronidase  (reporter gene) staining was also observed in the pollen grains and at the base  of the corolla.
authors.affiliation: School of Biological Sciences, University of Wales, Bangor, Gwynedd, United Kingdom. georgios.merkouropoulos@gmail.com
authors.forename: G
authors.initials: G
authors.lastname: Merkouropoulos
authors.forename: A H
authors.initials: AH
authors.lastname: Shirsat
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D035264:Flowers / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics*; D005966:Glucuronidase / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018547:Plant Stems / Q000166:cytology / Q000378:metabolism; D030821:Plants, Genetically Modified; D053205:Pollen Tube / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding; D014026:Nicotiana / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D006023:Glycoproteins; D010940:Plant Proteins; D014157:Transcription Factors; C005488:extensin protein, plant; D005966:Glucuronidase