title: Expression of nuclear factor one A and -B in the olfactory bulb.
abstract: The nuclear factor one (NFI) family of transcription factors consists of four members in vertebrates, NFIA, NFIB, NFIC, and NFIX, which share a highly conserved N-terminal DNA-binding domain. NFI genes are widely expressed in the developing mouse brain, and mouse mutants lacking NFIA, NFIB, or NFIX exhibit developmental deficits in several areas, including the cortex, hippocampus, pons, and cerebellum. Here we analyzed the expression of NFIA and NFIB in the developing and adult olfactory bulb (OB), rostral migratory stream (RMS), and subventricular zone (SVZ). We found that NFIA and NFIB are expressed within these regions during embryonic and postnatal development and in the adult. Immunohistochemical analysis using cell-type-specific markers revealed that migrating neuroblasts in the adult brain express NFI transcription factors, as do astrocytes within the RMS and progenitor cells within the SVZ. Moreover, astrocytes within the OB express NFIA, whereas mitral cells within the OB express NFIB. Taken together these data show that NFIA and NFIB are expressed in both the developing and the adult OB and in the RMS and SVZ, indicative of a regulatory role for these transcription factors in the development of this facet of the olfactory system.
authors.affiliation: The University of Maryland, Baltimore, School of Medicine, Baltimore, Maryland 21201-1559, USA. cplac001@umaryland.edu
authors.forename: Céline
authors.initials: C
authors.lastname: Plachez
authors.forename: Kathleen
authors.initials: K
authors.lastname: Cato
authors.forename: Robert C
authors.initials: RC
authors.lastname: McLeay
authors.forename: Yee Hsieh Evelyn
authors.initials: YH
authors.lastname: Heng
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Bailey
authors.forename: Richard M
authors.initials: RM
authors.lastname: Gronostajski
authors.forename: Richard M
authors.initials: RM
authors.lastname: Gronostasjki
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Richards
authors.forename: Adam C
authors.initials: AC
authors.lastname: Puche
authors.forename: Michael
authors.initials: M
authors.lastname: Piper
mesh_terms: D000367:Age Factors; D000818:Animals; D000918:Antibody Specificity; D002465:Cell Movement / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D007150:Immunohistochemistry; D020547:Lateral Ventricles / Q000196:embryology / Q000254:growth & development / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D051839:NFI Transcription Factors / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009830:Olfactory Bulb / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D011247:Pregnancy
chemical_list: D005904:Glial Fibrillary Acidic Protein; D051839:NFI Transcription Factors; C497646:Nfia protein, mouse; C484683:Nfib protein, mouse; D049452:Green Fluorescent Proteins