title: Extracellular calmodulin regulates growth and cAMP-mediated chemotaxis in Dictyostelium discoideum.
abstract: The existence of extracellular calmodulin (CaM) has had a long and controversial history. CaM is a ubiquitous calcium-binding protein that has been found in every eukaryotic cell system. Calcium-free apo-CaM and Ca(2+)/CaM exert their effects by binding to and regulating the activity of CaM-binding proteins (CaMBPs). Most of the research done to date on CaM and its CaMBPs has focused on their intracellular functions. The presence of extracellular CaM is well established in a number of plants where it functions in proliferation, cell wall regeneration, gene regulation and germination. While CaM has been detected extracellularly in several animal species, including frog, rat, rabbit and human, its extracellular localization and functions are less well established. In contrast the study of extracellular CaM in eukaryotic microbes remains to be done. Here we show that CaM is constitutively expressed and secreted throughout asexual development in Dictyostelium where the presence of extracellular CaM dose-dependently inhibits cell proliferation but increases cAMP mediated chemotaxis. During development, extracellular CaM localizes within the slime sheath where it coexists with at least one CaMBP, the matricellular CaM-binding protein CyrA. Coupled with previous research, this work provides direct evidence for the existence of extracellular CaM in the Dictyostelium and provides insight into its functions in this model amoebozoan.
authors.affiliation: Department of Cell and Systems Biology, University of Toronto, 25 Harbord St., Toronto, Ontario, Canada M5S 3G5. danton.oday@utoronto.ca
authors.forename: Danton H
authors.initials: DH
authors.lastname: O'Day
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Huber
authors.forename: Andres
authors.initials: A
authors.lastname: Suarez
mesh_terms: D002147:Calmodulin / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology; D049109:Cell Proliferation; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology*; D000242:Cyclic AMP / Q000494:pharmacology / Q000502:physiology*; D004023:Dictyostelium / Q000187:drug effects / Q000254:growth & development* / Q000502:physiology
chemical_list: D002147:Calmodulin; D000242:Cyclic AMP