title: Biotransformation of benzene and toluene to catechols by phenol hydroxylase from Arthrobacter sp. W1.
abstract: Phenol hydroxylase gene engineered microorganism (PHIND) was used to synthesize catechols from benzene and toluene by successive hydroxylation reaction. HPLC-MS and (1)H NMR analysis proved that the products of biotransformation were the corresponding catechols via the intermediate production of phenols. It was indicated that the main products of toluene oxidation were o-cresol and p-cresol. 3-Methylcatechol was the predominant product for m-cresol biotransformation. Formation rate of catechol (25 μM/min/g cell dry weight) was 1.43-fold higher than that of methylcatechols. It was suggested that phenol hydroxylase could be successfully used to transform both benzene and toluene to catechols by successive hydroxylation.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. hit_dlut@yahoo.cn
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Shi
authors.forename: Tie-Heng
authors.initials: TH
authors.lastname: Sun
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Ji-Ti
authors.initials: JT
authors.lastname: Zhou
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Qu
mesh_terms: D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics; D001554:Benzene / Q000378:metabolism*; D001711:Biotransformation; D002396:Catechols / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D060847:Metabolic Engineering; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014050:Toluene / Q000378:metabolism*
chemical_list: D002396:Catechols; D004269:DNA, Bacterial; D014050:Toluene; D006899:Mixed Function Oxygenases; C022336:phenol 2-monooxygenase; D001554:Benzene