title: Role of KATP channels and TRPV1 receptors in hydrogen sulfide-enhanced gastric emptying of liquid in awake mice.
abstract: Hydrogen sulphide (H(2)S) has shown to relax gastrointestinal muscle. Here in, we evaluated the effects of H(2)S donors on gastric emptying and in pyloric sphincter muscle relaxation, and whether these effects involved K(ATP) channels or TRPV1 receptors. Mice were treated with l-cysteine (alone or with propargylglycine-an inhibitor of H(2)S synthesis), NaHS, Lawesson's reagent (H(2)S donors) or saline. After 30 min, mice were gavaged with a liquid meal containing a nonabsorbable marker and then killed at 10, 20 or 30 min intervals to assess marker recovery from the stomach and intestine. This experiment was repeated in mice pre-treated with K(ATP) channel (glibenclamide) or TRPV1 receptor (capsazepine) antagonists. In addition, pyloric sphincter muscles were mounted in an organ bath, incubated with saline, glibenclamide or capsazepine, and NaHS dose-responses were determined. H(2)S donors and l-cysteine enhanced gastric emptying in a dose-dependent manner; propargylglycine reversed the effect of l-cysteine. Both glibenclamide and capsazepine abolished l-cysteine and H(2)S donors' augmentation of gastric emptying. Dose-dependent inductions of pyloric sphincter relaxation by NaHS were abolished by glibenclamide or capsazepine. These data suggest that H(2)S donors-induced acceleration of gastric emptying and relaxation of pyloric sphincter muscle by K(ATP) channel and TRPV1 receptor activations.
authors.affiliation: Institute of Biomedicine of Brazilian Semi-Arid (INCT-IBISAB), Departament of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, CEP: 60430-270, Fortaleza, CE, Brazil.
authors.forename: Jand Venes R
authors.initials: JV
authors.lastname: Medeiros
authors.forename: Víctor H
authors.initials: VH
authors.lastname: Bezerra
authors.forename: Larisse T
authors.initials: LT
authors.lastname: Lucetti
authors.forename: Roberto César P
authors.initials: RC
authors.lastname: Lima-Júnior
authors.forename: André Luiz R
authors.initials: AL
authors.lastname: Barbosa
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Tavares
authors.forename: Pedro Jorge C
authors.initials: PJ
authors.lastname: Magalhães
authors.forename: Armênio A
authors.initials: AA
authors.lastname: Santos
authors.forename: Fernando Q
authors.initials: FQ
authors.lastname: Cunha
authors.forename: Pedro Marcos G
authors.initials: PM
authors.lastname: Soares
authors.forename: Marcellus H L P
authors.initials: MH
authors.lastname: Souza
mesh_terms: D000818:Animals; D002211:Capsaicin / Q000031:analogs & derivatives / Q000494:pharmacology; D005746:Gastric Emptying / Q000187:drug effects / Q000502:physiology*; D005905:Glyburide / Q000494:pharmacology; D006862:Hydrogen Sulfide; D054086:KATP Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D008297:Male; D051379:Mice; D009126:Muscle Relaxation / Q000187:drug effects; D026902:Potassium Channel Blockers / Q000494:pharmacology; D011708:Pylorus / Q000187:drug effects / Q000502:physiology; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D054086:KATP Channels; D026902:Potassium Channel Blockers; D050916:TRPV Cation Channels; C494484:TRPV1 receptor; C071423:capsazepine; D002211:Capsaicin; D005905:Glyburide; D006862:Hydrogen Sulfide