title: Chemical silicon surface modification and bioreceptor attachment to develop competitive integrated photonic biosensors.
abstract: Methodology for the functionalization of silicon-based materials employed for the development of photonic label-free nanobiosensors is reported. The studied functionalization based on organosilane chemistry allowed the direct attachment of biomolecules in a single step, maintaining their bioavailability. Using this immobilization approach in probe microarrays, successful specific detection of bacterial DNA is achieved, reaching hybridization sensitivities of 10 pM. The utility of the immobilization approach for the functionalization of label-free nanobiosensors based on photonic crystals and ring resonators was demonstrated using bovine serum albumin (BSA)/anti-BSA as a model system.
authors.affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Escorihuela
authors.forename: María José
authors.initials: MJ
authors.lastname: Bañuls
authors.forename: Javier
authors.initials: J
authors.lastname: García Castelló
authors.forename: Veronica
authors.initials: V
authors.lastname: Toccafondo
authors.forename: Jaime
authors.initials: J
authors.lastname: García-Rupérez
authors.forename: Rosa
authors.initials: R
authors.lastname: Puchades
authors.forename: Ángel
authors.initials: Á
authors.lastname: Maquieira
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation*; D002417:Cattle; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004867:Equipment Design; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry; D007368:Interferometry; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D055095:Optics and Photonics / Q000295:instrumentation*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000906:Antibodies; D004269:DNA, Bacterial; D055767:Immobilized Proteins; D012710:Serum Albumin, Bovine; D012825:Silicon