title: [Effects of heat shock protein 70-2 gene polymorphisms on the transcription of HSP 70-2 mRNA and the translation of HSP 70 protein in lung cancer].
abstract: OBJECTIVE
  This study aimed to investigate the effects of gene polymorphism of heat shock protein 70-2 (HSP 70-2) 1267A/G on the mRNA level HSP 70-2 mRNA and the protein level HSP 70 in human lung cancer.
  
  
  METHODS
  Forty six lung cancer patients diagnosed histopathologically between February and August 2008 from a hospital in zhengzhou were enrolled as the subjects in this study. Gene polymorphism of HSP 70-2 1276A/G in 46 patients with lung cancer was detected by PCR-RFLP. The mRNA levels of HSP 70-2 mRNA and the protein levels of HSP 70 in lung tissue and para-cancerous tissues of these subjects were determined by RT-PCR and Western blotting respectively.
  
  
  RESULTS
  The expression levels of HSP 70-2 mRNA (1.02 ± 0.30) and HSP 70 protein (0.44 ± 0.12) in the lung cancer tissues was significantly higher than that in para-cancerous tissues (0.19 ± 0.04, 0.12 ± 0.02). The relative levels of HSP 70-2 mRNA in the subjects with AA genotype (1.32 ± 0.22) were significantly higher than the patients with AG genotype or GG genotype (0.95 ± 0.17, 0.70 ± 0.16) at the site of 1267 (A/G) (P < 0.01); however, the relative protein levels of HSP 70 were 0.47 ± 0.13 (AA genotype), 0.42 ± 0.11 (AG genotype), 0.45 ± 0.11 (GG genotype), respectively, which showed no statistically significant difference (P > 0.05).
  
  
  CONCLUSIONS
  The polymorphism of HSP 70-2 1267 (A/G) is highly associated with the transcription level of HSP 70-2 mRNA, but not with the expression level of HSP 70 protein.
authors.affiliation: Department of Occupational Health, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Hao-quan
authors.initials: HQ
authors.lastname: Lu
authors.forename: Yu-zhen
authors.initials: YZ
authors.lastname: Wang
authors.forename: Peng-hui
authors.initials: PH
authors.lastname: Sun
authors.forename: Shou-pei
authors.initials: SP
authors.lastname: Liang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Xiao-long
authors.initials: XL
authors.lastname: Wang
authors.forename: Dong
authors.initials: D
authors.lastname: Xu
authors.forename: Wu
authors.initials: W
authors.lastname: Yao
authors.forename: Yi-ming
authors.initials: YM
authors.lastname: Wu
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D005260:Female; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018840:HSP70 Heat-Shock Proteins; C525621:HSPA1B protein, human; D012333:RNA, Messenger