title: NLF2 gene expression in the endometrium of patients with implantation failure after IVF treatment.
abstract: The aim of this study was to analyze the expression of microfibril-associated protein 2 (MFAP2), microfibril-associated protein 5 (MFAP5) and nuclear localized factor 2 (NLF2) genes in patients with repeated IVF failure and compare with fertile population. Total RNA was isolated from 38 patients (repeated implantation failure, group 1, n=22; fertile patients, group 2, n=16). mRNA expression levels were measured quantitatively using real-time polymerase chain reaction. Our results showed that mRNA expression of NLF2 significantly decreased in the infertility group as compared to control group (P=0.023). In addition a marked decrease was observed in the expression of MFAP2 in women with repeated implantation failure. In conclusion, NLF2 gene expression levels and differences in MFAP2 and MFAP5 gene expressions (albeit being insignificant) between infertile group and control group draw attention to a genetic basis under implantation failure.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Gazi University, 06500 Besevler, Ankara, Turkey.
authors.forename: Esengul
authors.initials: E
authors.lastname: Turkyilmaz
authors.forename: Haldun
authors.initials: H
authors.lastname: Guner
authors.forename: Mehmet
authors.initials: M
authors.lastname: Erdem
authors.forename: Ahmet
authors.initials: A
authors.lastname: Erdem
authors.forename: Aydan Asyali
authors.initials: AA
authors.lastname: Biri
authors.forename: Ece
authors.initials: E
authors.lastname: Konac
authors.forename: Ebru
authors.initials: E
authors.lastname: Alp
authors.forename: Hacer Ilke
authors.initials: HI
authors.lastname: Onen
authors.forename: Sevda
authors.initials: S
authors.lastname: Menevse
mesh_terms: D000328:Adult; D003285:Contractile Proteins / Q000235:genetics; D010064:Embryo Implantation / Q000235:genetics*; D004717:Endometrium / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005260:Female; D005298:Fertility / Q000235:genetics*; D005307:Fertilization in Vitro; D006801:Humans; D007247:Infertility, Female / Q000235:genetics* / Q000628:therapy*; D008297:Male; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C494528:C2CD4B protein, human; D003285:Contractile Proteins; D016326:Extracellular Matrix Proteins; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D012333:RNA, Messenger; D014157:Transcription Factors; C016598:microfibrillar protein