title: Note: in vivo pH imaging system using luminescent indicator and color camera.
abstract: Microscopic in vivo pH imaging system is developed that can capture the luminescent- and color-imaging. The former gives a quantitative measurement of a pH distribution in vivo. The latter captures the structural information that can be overlaid to the pH distribution for correlating the structure of a specimen and its pH distribution. By using a digital color camera, a luminescent image as well as a color image is obtained. The system uses HPTS (8-hydroxypyrene-1,3,6-trisulfonate) as a luminescent pH indicator for the luminescent imaging. Filter units are mounted in the microscope, which extract two luminescent images for using the excitation-ratio method. A ratio of the two images is converted to a pH distribution through a priori pH calibration. An application of the system to epidermal cells of Lactuca Sativa L is shown.
authors.affiliation: Aerospace Research and Development Directorate, Japan Aerospace Exploration Agency, Chofu, Tokyo 182-8522, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Sakaue
authors.forename: Risako
authors.initials: R
authors.lastname: Dan
authors.forename: Megumi
authors.initials: M
authors.lastname: Shimizu
authors.forename: Haruko
authors.initials: H
authors.lastname: Kazama
mesh_terms: D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006863:Hydrogen-Ion Concentration; D008029:Lighting / Q000295:instrumentation*; D008163:Luminescent Measurements / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*