title: Characterization of a protein conjugate using an asymmetrical-flow field-flow fractionation and a size-exclusion chromatography with multi-detection system.
abstract: In this study we present detailed characterization of a protein-PEG conjugate using two separation techniques, that is, asymmetrical-flow field-flow fractionation (AF4) and size-exclusion chromatography (SEC), which were online coupled to a series of successively connected detectors: an ultraviolet, a multiangle light-scattering, a quasi-elastic light-scattering, and a refractive-index detector (UV-MALS(QELS)-RI). Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used as a complementary characterization technique. The results of AF4 as well as SEC on two columns connected in series, with both separation techniques coupled to a multidetection system, indicate the uniform molar mass and chemical composition of the conjugate, that is, the molar ratio of protein to PEG is 1/1, the presence of minute amounts of residual unreacted protein and the aggregates with the same chemical composition as that of the conjugate. Since the portion of aggregated species is smaller in the acetate buffer solution containing 5% sorbitol than in the acetate buffer solution with 200-mM sodium chloride, the former buffer solution is more suitable for conjugate storage. The separation using only one SEC column results in poorly resolved peaks of the PEGylated protein conjugate and the aggregates, whereas MALDI-TOF MS analysis reveal the presence of the residual protein, but not the aggregates.
authors.affiliation: National Institute of Chemistry, Ljubljana, Slovenia.
authors.forename: Katja
authors.initials: K
authors.lastname: Rebolj
authors.forename: David
authors.initials: D
authors.lastname: Pahovnik
authors.forename: Ema
authors.initials: E
authors.lastname: Zagar
mesh_terms: D000085:Acetates / Q000737:chemistry; D002021:Buffers; D002850:Chromatography, Gel; D011092:Polyethylene Glycols / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry; D013012:Sorbitol / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000085:Acetates; D002021:Buffers; D011506:Proteins; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D013012:Sorbitol