title: Sex-dependent association of phosphodiesterase 4D gene polymorphisms with ischemic stroke in Henan Han population.
abstract: BACKGROUND
  Recent evidence has implicated the gene for phosphodiesterase 4D (PDE4D) as susceptibility gene for ischemic stroke (IS) in Icelandic population. However, there are few reports on the associations between PDE4D gene polymorphisms and IS in Chinese individuals. The present study aimed to investigate the possible association of genetic polymorphisms in PDE4D gene with IS in Henan Han population.
  
  
  METHODS
  A total of 400 patients with IS and 400 matched controls were examined using a case-control design. Two single nucleotide polymorphism (SNPs) (rs918592 and rs2910829) in PDE4D gene were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Odds ratios (OR) and 95% confidence intervals (95%CI) were calculated to test the association between the genetic factors and IS. Genetic parameter and association studies were carried out with SPSS 16.0.
  
  
  RESULTS
  Among the two SNPs tested, the rs918592 was significantly associated with IS (OR: 1.351, 95%CI: 1.110 - 1.645), especially in male patients (OR: 1.427, 95%CI: 1.105 - 1.844). Haplotype analysis showed that A-T was associated with an increased risk of the IS (OR: 2.114, 95%CI: 2.005 - 2.230) while G-T was associated with decreased risk of IS (OR: 0.419, 95%CI: 0.302 - 0.583). Protecting effect of haplotype G-T was also significant in males (OR: 0.264, 95%CI: 0.162 - 0.431).
  
  
  CONCLUSIONS
  The present study demonstrated a strong association of rs918592 with IS. Haplotype A-T increased the risk of IS while haplotype G-T had a protective effect in Henan Han population. The association was sex-dependent with male patients showing stronger effect.
authors.affiliation: Department of Cell Biology and Medical Genetics, Basic Medical College of Zhengzhou University, Zhengzhou, Henan 450052, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.forename: Jun-Yu
authors.initials: JY
authors.lastname: Bai
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Song
authors.initials: S
authors.lastname: Tan
authors.forename: Yin-Shu
authors.initials: YS
authors.lastname: Chang
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Cong-Cong
authors.initials: CC
authors.lastname: Shi
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Qing-Chuan
authors.initials: QC
authors.lastname: Feng
authors.forename: Hua
authors.initials: H
authors.lastname: Qi
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Song
authors.forename: Hong
authors.initials: H
authors.lastname: Zheng
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Xu
mesh_terms: D000368:Aged; D002545:Brain Ischemia / Q000235:genetics*; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012737:Sex Factors; D020521:Stroke / Q000235:genetics*
chemical_list: D054703:Cyclic Nucleotide Phosphodiesterases, Type 4