title: Autologous peripheral blood hematopoietic cell transplantation in dogs with B-cell lymphoma.
abstract: BACKGROUND
  Peripheral blood CD34+ hematopoietic cell transplantation (PBHCT) is commonly used to treat human patients with relapsed non-Hodgkin diffuse, large B-cell lymphoma with cure rates approaching 50%.
  
  
  OBJECTIVE
  To determine the safety and feasibility of performing PBHCT to treat canine B-cell lymphoma (LSA) patients in a clinical academic setting.
  
  
  METHODS
  Twenty-four client-owned dogs diagnosed with B-cell LSA.
  
  
  METHODS
  After high-dose cyclophosphamide and rhG-colony-stimulating factor treatment, peripheral blood mononuclear cells were collected using cell separator machines. The harvested cells then were infused after a 10 Gy dose of total body irradiation (TBI). Post-irradiation adverse effects were managed symptomatically and dogs were discharged upon evidence of engraftment.
  
  
  RESULTS
  More than 2 × 10(6) CD34+ cells/kg were harvested in 23/24 dogs. Preapheresis peripheral blood monocyte count was correlated with the number of CD34+ cells/kg harvested. Twenty-one of 24 (87.5%) dogs engrafted appropriately, whereas 2 dogs (8.3%) died in the hospital. One (5%) dog exhibited delayed engraftment and died 45 days after PBHCT. One dog developed presumed TBI-induced pulmonary fibrosis approximately 8 months after PBHCT. The median disease-free interval and overall survival (OS) of all dogs from the time of PBHCT was 271 and 463 days, respectively. Five of 15 (33%) dogs transplanted before they relapsed remain in clinical remission for their disease at a median OS of 524 days (range, 361-665 days).
  
  
  CONCLUSIONS
  In most cases, PBHCT led to complete hematologic reconstitution. Therefore, PBHCT may be considered as a treatment option for dogs with B-cell lymphoma.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 1051 William Moore Drive, Raleigh, NC 27607, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Willcox
authors.forename: A
authors.initials: A
authors.lastname: Pruitt
authors.forename: S E
authors.initials: SE
authors.lastname: Suter
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000662:veterinary; D018572:Disease-Free Survival; D004283:Dog Diseases / Q000473:pathology / Q000601:surgery*; D004285:Dogs; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D053208:Kaplan-Meier Estimate; D016393:Lymphoma, B-Cell / Q000473:pathology / Q000601:surgery / Q000662:veterinary*; D008297:Male; D014916:Whole-Body Irradiation / Q000662:veterinary