title: Single-drop optimization of protein crystallization.
abstract: A completely new crystal-growth device has been developed that permits charting a course across the phase diagram to produce crystalline samples optimized for diffraction experiments. The utility of the device is demonstrated for the production of crystals for the traditional X-ray diffraction data-collection experiment, of microcrystals optimal for data-collection experiments at a modern microbeam insertion-device synchrotron beamline and of nanocrystals required for data collection on an X-ray laser beamline.
authors.affiliation: Laboratory for Structural Biology of Infection and Inflammation, Center for Structural and Cell Biology in Medicine, Institute of Biochemistry, University of Lübeck, c/o DESY, Building 22a, Notkestrasse 85, 22603 Hamburg, Germany.
authors.forename: Arne
authors.initials: A
authors.lastname: Meyer
authors.forename: Karsten
authors.initials: K
authors.lastname: Dierks
authors.forename: Dierk
authors.initials: D
authors.lastname: Hilterhaus
authors.forename: Thomas
authors.initials: T
authors.lastname: Klupsch
authors.forename: Peter
authors.initials: P
authors.lastname: Mühlig
authors.forename: Jens
authors.initials: J
authors.lastname: Kleesiek
authors.forename: Robert
authors.initials: R
authors.lastname: Schöpflin
authors.forename: Howard
authors.initials: H
authors.lastname: Einspahr
authors.forename: Rolf
authors.initials: R
authors.lastname: Hilgenfeld
authors.forename: Christian
authors.initials: C
authors.lastname: Betzel
mesh_terms: D000818:Animals; D003435:Crotalid Venoms / Q000032:analysis*; D017839:Crotalus; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000379:methods*; D032426:Marantaceae / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis*; D054790:Ribosome Inactivating Proteins, Type 2 / Q000032:analysis*; D014118:Toxins, Biological / Q000032:analysis*; D028182:Viscum album / Q000737:chemistry
chemical_list: D003435:Crotalid Venoms; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C488862:mistletoe lectin I; C010563:crotamine