title: Adaptation of a Chinese ferret badger strain of rabies virus to high-titered growth in BHK-21 cells for canine vaccine development.
abstract: Rabies virus strain JX08-45CC was derived from a Chinese isolate (JX08-45) by serial passage in the BHK-21 cell line, reaching a titer of 10(8) TCID(50)/mL. JX08-45CC produced rabies in adult mice but was nonpathogenic in dogs after intramuscular injection. A comparison of the entire genomes of JX08-45 and JX08-45CC led to the identification of 17 nucleotide substitutions, resulting in seven amino acid changes in the mature G and L proteins. The immunogenicity of β-propiolactone-inactivated JX08-45CC was similar to the immunogenicity of the live vaccine strains widely used in China. The inactivated vaccine induced antibody responses for more than 6 months and provided full protection from an intramuscular challenge in dogs. JX08-45CC has excellent potential for development as an inactivated vaccine for dogs in China.
authors.affiliation: Laboratory of Epidemiology, Military Veterinary Research Institute, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Liu
authors.forename: Shoufeng
authors.initials: S
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.forename: Rongliang
authors.initials: R
authors.lastname: Hu
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000821:virology; D002460:Cell Line; D002681:China; D006224:Cricetinae; D004283:Dog Diseases / Q000517:prevention & control*; D004285:Dogs; D016679:Genome, Viral; D051379:Mice; D045969:Mustelidae / Q000821:virology*; D012367:RNA, Viral; D011818:Rabies / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000276:immunology; D011897:Random Allocation; D012692:Serial Passage; D013045:Species Specificity; D014613:Vaccines, Attenuated / Q000276:immunology
chemical_list: D012367:RNA, Viral; D011819:Rabies Vaccines; D014613:Vaccines, Attenuated