title: DNA aptamers detecting generic amyloid epitopes.
abstract: Amyloids are fibrillar protein aggregates resulting from non-covalent autocatalytic polymerization of various structurally and functionally unrelated proteins. Previously we have selected DNA aptamers, which bind specifically to the in vitro assembled amyloid fibrils of the yeast prionogenic protein Sup35. Here we show that such DNA aptamers can be used to detect SDS-insoluble amyloid aggregates of the Sup35 protein, and of some other amyloidogenic proteins, including mouse PrP, formed in yeast cells. The obtained data suggest that these aggregates and the Sup35 amyloid fibrils assembled in vitro possess common conformational epitopes recognizable by aptamers. The described DNA aptamers may be used for detection of various amyloid aggregates in yeast and, presumably, other organisms.
authors.affiliation: A.N. Bach Institute of Biochemistry, The Russian Academy of Sciences, Moscow, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Mitkevich
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Kochneva-Pervukhova
authors.forename: Elizaveta R
authors.initials: ER
authors.lastname: Surina
authors.forename: Sergei V
authors.initials: SV
authors.lastname: Benevolensky
authors.forename: Vitaly V
authors.initials: VV
authors.lastname: Kushnirov
authors.forename: Michael D
authors.initials: MD
authors.lastname: Ter-Avanesyan
mesh_terms: D000682:Amyloid / Q000032:analysis / Q000378:metabolism*; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D010454:Peptide Termination Factors / Q000032:analysis / Q000378:metabolism*; D011328:Prions / Q000032:analysis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000378:metabolism*; D012995:Solubility
chemical_list: D000682:Amyloid; D052157:Aptamers, Nucleotide; D010454:Peptide Termination Factors; D011328:Prions; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins