title: [Construction of a red fluorescent shuttle vector controlled by recA operon promoter of Streptococcus mutans].
abstract: OBJECTIVE
  To construct a red fluorescent shuttle vector controlled by recA operon promoter to transform Streptococcus mutans.
  
  
  METHODS
  The promoter of recA was amplified from Streptococcus mutans UA159, and connected to plasmid pDsRed2-N1 to construct pRred with a red fluorescent coding gene, which was then inserted into the shuttle vector pDL276 to construct pLRred.
  
  
  RESULTS
  pLRred was successfully constructed, and Escherichia coli transformed with the pLRred plasmid could express reporter gene DsRed.
  
  
  CONCLUSIONS
  The recombination plasmid pLRred can be used in the further research of the expression of cariogenic virulence factor gene by Streptococcus mutans in biofilm.
authors.affiliation: Department of Oral Medicine, The Affiliated Hospital of Stomatology, Zunyi Medical College, Zunyi Guizhou 563003, China.
authors.forename: Wen-ming
authors.initials: WM
authors.lastname: Huang
authors.forename: Yang-long
authors.initials: YL
authors.lastname: Xu
authors.forename: De-qin
authors.initials: DQ
authors.lastname: Yang
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005456:Fluorescent Dyes; D020043:Genes, Essential; D017930:Genes, Reporter; D005822:Genetic Vectors; D008164:Luminescent Proteins / Q000235:genetics*; D009876:Operon; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011938:Rec A Recombinases / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013295:Streptococcus mutans / Q000235:genetics*; D014169:Transformation, Bacterial; D000097573:Red Fluorescent Protein
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D011938:Rec A Recombinases; D011994:Recombinant Proteins; D000097573:Red Fluorescent Protein