title: Isolation and characterization of pullulan-degrading Anoxybacillus species isolated from Malaysian hot springs.
abstract: Two thermophilic bacteria (SK3-4 and DT3-1) were isolated from the Sungai Klah (SK) and Dusun Tua (DT) hot springs in Malaysia. The cells from both strains were rod-shaped, stained Gram positive and formed endospores. The optimal growth of both strains was observed at 55 degrees C and pH 7. Strain DT3-1 exhibited a higher tolerance to chloramphenicol (100 microg ml(-1)) but showed a lower tolerance to sodium chloride (2%, w/v) compared to strain SK3-4. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both strains belong to the genus Anoxybacillus. High concentrations of 15:0 iso in the fatty acid profiles support the conclusion that both strains belong to the genus Anoxybacillus and exhibit unique fatty acid compositions and percentages compared to other Anoxybacillus species. The DNA G + C contents were 42.0 mol% and 41.8 mol% for strains SK3-4 and DT3-1, respectively. Strains SK3-4 and DT3-1 were able to degrade pullulan and to produce maltotriose and glucose, respectively, as their main end products. Based on phenotypic and chemotaxonomic characteristics, 16S rRNA gene sequences, and the DNA G + C content, we propose that strains SK3-4 and DT3-1 are new pullulan-degrading Anoxybacillus strains.
authors.affiliation: Department of Biological Sciences, Faculty of Biosciences and Bioengineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.
authors.forename: Yen Yen
authors.initials: YY
authors.lastname: Chai
authors.forename: Ummirul Mukminin
authors.initials: UM
authors.lastname: Kahar
authors.forename: Madihah
authors.initials: M
authors.lastname: Md Salleh
authors.forename: Rosli
authors.initials: R
authors.lastname: Md Illias
authors.forename: Kian Mau
authors.initials: KM
authors.lastname: Goh
mesh_terms: D058677:Anoxybacillus / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism* / Q000648:ultrastructure; D001482:Base Composition; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005936:Glucans / Q000378:metabolism*; D045482:Hot Springs / Q000382:microbiology*; D008296:Malaysia
chemical_list: D005227:Fatty Acids; D005936:Glucans; C009109:pullulan