title: [Experiment study of SDF-1 expression during the survival process of the narrow pedicle flap with hypoxia and ischemia].
abstract: OBJECTIVE
  To detect the stromal cell derived factor 1 (SDF-1) expression during the survival process of the narrow pedicle flap with hypoxia and ischemia and to investigate the role of SDF-1/ CXCR4 axis in flap neovascularization.
  
  
  METHODS
  The narrow pedicle flaps were formed on the bilateral back of 5 pigs. The pedicle ratio of length to width was 4:2. The flap size was 2 cm x 2 cm (group A), 3 cm x 3 cm (group B), 4 cm x 4 cm (group C), 5 cm x 5 cm(group D), 6 cm x6 cm (group E). The flaps survival rate was observed and HE staining was performed. The SDF-1 expression at the distal end of flaps was detected by ELISA during the operation and 3, 5, 7, 14 days after operation.
  
  
  RESULTS
  (1) SDF-1 expression at the same group increased after operation until it reached the peak value at 5 days after operation; then it decreased to basic value. (2) SDF-1 expression in different groups was higher in bigger flaps until the flaps size reached 5 cm x 5 cm. Then partial necrosis happened at the distal end of flaps.
  
  
  CONCLUSIONS
  The SDF-1 expression may be related to the blood supply during the survival process of the narrow pedicle flap with hypoxia and ischemia.
authors.affiliation: Department of Plastic Surgery,the Second Affiliated Hospital of Soochow University, Suzhou 215004, China.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Wu
authors.forename: Tian-lan
authors.initials: TL
authors.lastname: Zhao
authors.forename: Dao-jiang
authors.initials: DJ
authors.lastname: Yu
authors.forename: Wei-bao
authors.initials: WB
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000502:physiology*; D054377:Chemokine CXCL12 / Q000378:metabolism*; D006085:Graft Survival / Q000502:physiology*; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D015398:Signal Transduction; D013524:Surgical Flaps / Q000098:blood supply / Q000502:physiology*; D034421:Sus scrofa; D013552:Swine
chemical_list: D054377:Chemokine CXCL12