title: Argocytes containing enzyme nanoparticles reduce toxic concentrations of arginine in the blood.
abstract: A method for incorporation of arginase nanoparticles into mouse erythrocytes has been developed and the possibility of reducing blood arginine concentration in animals with experimental hyperargininemia with arginase-loaded erythrocytes (argocytes) has been studied. Argocyte infusion to animals with hyperargininemia led to a rapid decrease in blood arginine concentration within 1 h and this effect of argocytes persisted for at least 4 h. This was paralleled by an increase in plasma concentrations of urea and ornithine. Hence, plasma arginine is hydrolyzed by arginase incorporated into argocytes; argocytes are functionally active and can serve as a defense system in pathological hyperargininemia, while the method developed by us can be regarded as a new nanobiotechnology for medicine and veterinary.
authors.affiliation: Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia. kaminsky@iteb.ru
authors.forename: Yu G
authors.initials: YG
authors.lastname: Kaminsky
authors.forename: E A
authors.initials: EA
authors.lastname: Kosenko
mesh_terms: D000818:Animals; D001119:Arginase / Q000008:administration & dosage / Q000737:chemistry / Q000627:therapeutic use*; D001120:Arginine / Q000097:blood*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism*; D020162:Hyperargininemia / Q000188:drug therapy*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology
chemical_list: D001120:Arginine; D001119:Arginase