title: Studying Salmonellae and Yersiniae host-pathogen interactions using integrated 'omics and modeling.
abstract: Salmonella and Yersinia are two distantly related genera containing species with wide host-range specificity and pathogenic capacity. The metabolic complexity of these organisms facilitates robust lifestyles both outside of and within animal hosts. Using a pathogen-centric systems biology approach, we are combining a multi-omics (transcriptomics, proteomics, metabolomics) strategy to define properties of these pathogens under a variety of conditions including those that mimic the environments encountered during pathogenesis. These high-dimensional omics datasets are being integrated in selected ways to improve genome annotations, discover novel virulence-related factors, and model growth under infectious states. We will review the evolving technological approaches toward understanding complex microbial life through multi-omic measurements and integration, while highlighting some of our most recent successes in this area.
authors.affiliation: Biological Separations and Mass Spectroscopy Group, Pacific Northwest National Laboratory, PO Box 999, MSIN: K8-98, Richland, WA, 99352, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Ansong
authors.forename: Brooke L
authors.initials: BL
authors.lastname: Deatherage
authors.forename: Daniel
authors.initials: D
authors.lastname: Hyduke
authors.forename: Brian
authors.initials: B
authors.lastname: Schmidt
authors.forename: Jason E
authors.initials: JE
authors.lastname: McDermott
authors.forename: Marcus B
authors.initials: MB
authors.lastname: Jones
authors.forename: Sadhana
authors.initials: S
authors.lastname: Chauhan
authors.forename: Pep
authors.initials: P
authors.lastname: Charusanti
authors.forename: Young-Mo
authors.initials: YM
authors.lastname: Kim
authors.forename: Ernesto S
authors.initials: ES
authors.lastname: Nakayasu
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Afshan
authors.initials: A
authors.lastname: Kidwai
authors.forename: George
authors.initials: G
authors.lastname: Niemann
authors.forename: Roslyn N
authors.initials: RN
authors.lastname: Brown
authors.forename: Thomas O
authors.initials: TO
authors.lastname: Metz
authors.forename: Kathleen
authors.initials: K
authors.lastname: McAteer
authors.forename: Fred
authors.initials: F
authors.lastname: Heffron
authors.forename: Scott N
authors.initials: SN
authors.lastname: Peterson
authors.forename: Vladimir
authors.initials: V
authors.lastname: Motin
authors.forename: Bernhard O
authors.initials: BO
authors.lastname: Palsson
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Joshua N
authors.initials: JN
authors.lastname: Adkins
mesh_terms: D000818:Animals; D023281:Genomics; D054884:Host-Pathogen Interactions; D006801:Humans; D055432:Metabolomics; D040901:Proteomics; D012475:Salmonella / Q000472:pathogenicity*; D049490:Systems Biology / Q000379:methods*; D015007:Yersinia / Q000472:pathogenicity*