title: ¹H, ¹³C and ¹⁵N resonance assignment for the human K-Ras at physiological pH.
abstract: K-Ras, a member of the Ras family of small GTPases, is involved in cell growth, proliferation, differentiation and apoptosis and is frequently mutated in cancer. The activity of Ras is mediated by the inter-conversion between GTP- and GDP- bound states. This conversion is regulated by binding of effector proteins such as guanine nucleotide exchange factors and GTPase activating proteins. Previously, NMR signals from these effector-binding regions of Ras often remained unassigned and largely unobservable due to conformational exchange and polysterism inherent to this protein. In this paper, we report the complete backbone and C(β), as well as partial H(α), H(β) and C(γ), NMR assignment for human K-Ras (residues 1-166) in the GDP-bound form at a physiological pH of 7.4. These data thereby make possible detailed monitoring of the functional cycle of Ras and its interactions with nucleotides and effector proteins through the observation of fingerprint signals from all the functionally important regions of the protein.
authors.affiliation: Faculty of Life Sciences, Manchester Interdisciplinary Biocentre, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK.
authors.forename: Uybach
authors.initials: U
authors.lastname: Vo
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Embrey
authors.forename: Alexander L
authors.initials: AL
authors.lastname: Breeze
authors.forename: Alexander P
authors.initials: AP
authors.lastname: Golovanov
mesh_terms: D000595:Amino Acid Sequence; D002247:Carbon Isotopes; D006153:Guanosine Diphosphate / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D011518:Proto-Oncogene Proteins / Q000737:chemistry*; D016283:Proto-Oncogene Proteins p21(ras); D011522:Protons; D016415:Sequence Alignment; D018631:ras Proteins / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; C117307:KRAS protein, human; D009587:Nitrogen Isotopes; D011518:Proto-Oncogene Proteins; D011522:Protons; D006153:Guanosine Diphosphate; D016283:Proto-Oncogene Proteins p21(ras); D018631:ras Proteins