title: Protective efficacy of Mycobacterium indicus pranii against tuberculosis and underlying local lung immune responses in guinea pig model.
abstract: Tuberculosis kills two million people each year. As the current vaccine BCG fails to prevent adult cases of TB, an improved vaccine and/or vaccination strategy is urgently needed to combat TB. Previously we reported the higher protective efficacy of Mycobacterium indicus pranii (MIP), formerly known as Mycobacterium w (M.w) as compared to BCG in murine model of TB. In this study we further evaluated the protective efficacy of MIP in guinea pig model of TB. Modulation of post infection immune response was analyzed in the lungs of MIP immunized and control groups. We found reduced bacterial loads, improved pathology and organized granulomatous response at different post infection time points in the MIP-immunized group as compared to the BCG-immunized group. Combined results suggest that MIP-immunization results in heightened protective Th1 response as compared to BCG group, early after infection with M.tb and a balanced Th1 versus immunosuppressive response at late chronic stage of infection. The study demonstrates the higher antigen presenting cells function both inside the granuloma as well as in the single cell suspension of the lung in the MIP-immunized group. We further demonstrate that live MIP is safe to use in vivo as we observed quick clearance of MIP from the body and no untoward reaction was found. Aerosol route of immunization provided higher protection. Further this study provides evidence that MIP-immunization gives significantly better long term protection as compared to BCG against TB.
authors.affiliation: Product Development Cell 1, National Institute of Immunology, J.N.U Complex, Aruna Asaf Ali Marg, New Delhi 110067, India.
authors.forename: Ankan
authors.initials: A
authors.lastname: Gupta
authors.forename: F J
authors.initials: FJ
authors.lastname: Ahmad
authors.forename: Faiz
authors.initials: F
authors.lastname: Ahmad
authors.forename: U D
authors.initials: UD
authors.lastname: Gupta
authors.forename: M
authors.initials: M
authors.lastname: Natarajan
authors.forename: V M
authors.initials: VM
authors.lastname: Katoch
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Bhaskar
mesh_terms: D000818:Animals; D017209:Apoptosis; D058491:Bacterial Load; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D005260:Female; D006099:Granuloma / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D006168:Guinea Pigs; D008168:Lung / Q000276:immunology* / Q000473:pathology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D009161:Mycobacterium / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D032581:Tuberculosis Vaccines / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000473:pathology / Q000517:prevention & control*
chemical_list: D016207:Cytokines; D032581:Tuberculosis Vaccines