title: Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids.
abstract: In this work a chip-based nano HPLC coupled MS (HPLC-chip/MS) method with a simple sample preparation procedure was developed for the flavonoid profiling of soybean. The analytical properties of the method including the linearity (R(2) , 0.992-0.995), reproducibility (RSD, 1.50-7.66%), intraday precision (RSD, 1.41-5.14%) and interday precision (RSD, 2.76-16.90%) were satisfactory. Compared with the conventional HPLC/MS method, a fast extraction and analysis procedure was applied and more flavonoids were detected in a single run. Additionally, 13 flavonoids in soybean seed were identified for the first time. The method was then applied to the profiling of six varieties of soybean sowed at the same place. A clear discrimination was observed among different cultivars, three isoflavones, accounting for nearly 80% of total flavonoid contents, were found increased in the spring soybeans compared with the summer cultivars.
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Chang
authors.forename: Chunxia
authors.initials: C
authors.lastname: Zhao
authors.forename: Zeming
authors.initials: Z
authors.lastname: Wu
authors.forename: Jia
authors.initials: J
authors.lastname: Zhou
authors.forename: Sumin
authors.initials: S
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Lu
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis* / Q000302:isolation & purification; D016018:Least-Squares Analysis; D016014:Linear Models; D046208:Microchip Analytical Procedures / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000737:chemistry* / Q000145:classification; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D005419:Flavonoids