title: [Study on the relation between expression of angiotensin II receptor and apoptosis in myocardium in rats of endotoxemia].
abstract: OBJECTIVE
  To analyze the expression of angiotensin II (ANG II) receptor and apoptosis in myocardium in rats of endotoxemia.
  
  
  METHODS
  Model of endotoxemia was induced by intraperitoneal injection with lipopolysaccharide (LPS) 10 mg/kg in male Wistar rats and saline was injected into control group. The rats were killed at 2 h or 6 h after saline (control) or LPS . Expression of the correlation factors related to apoptosis of Bcl-2, Bax, AT1 and AT2 receptor in myocardial tissue were detected with immunohistochemistry (IHC), and changes of myocardial cells apoptosis was detected by the method of TUNEL. The gene expression of AT1 and AT2 receptor was examined by RT-PCR. The pathological changes of myocardial tissue were observed by electron microscope.
  
  
  RESULTS
  Compared with control group , the expression of AT1 and AT2 receptor were significantly decreased, especially in 6 h group; and the expression of Bcl-2 and Bax were decreased, the ratio of Bcl-2/Bax had the downtrend as well as the apoptosis of myocardial cells.
  
  
  CONCLUSIONS
  Interfered by LPS, the down regulation of AT1 and AT2 receptor expression has the negative relation with apoptosis of myocardial cells, this result indicated that down regulation of AT1 and AT2 receptor expression maybe related to cardiac functional impairment, which maybe help us to find a new protective path to prevent myocardial damage induced by systemic inflammatory.
authors.affiliation: The 2nd Department of Geratic Cardiolagy, Chinese PLA General Hospital, Beijing, China. xu_zh@163.com
authors.forename: Tie-hui
authors.initials: TH
authors.lastname: Xiao
authors.forename: Shi-wen
authors.initials: SW
authors.lastname: Wang
authors.forename: Yan-ming
authors.initials: YM
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Xin-yong
authors.initials: XY
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Ye
mesh_terms: D000818:Animals; D017209:Apoptosis; D019446:Endotoxemia / Q000378:metabolism*; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism*; D044139:Receptor, Angiotensin, Type 2 / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2