title: Fluorogenic assay of alkaline phosphatase activity based on the modulation of excited-state intramolecular proton transfer.
abstract: A new fluorogenic substrate 1, which enables the fast and quantitative analysis of alkaline phosphatase activity, has been developed. Selective enzymatic hydrolysis of 1 instantly generated fluorescent compound 2 in aqueous media, which undergo an excited-state intramolecular proton transfer process, resulting in a remarkable fluorescence turn-on signal with an unusually large Stokes shift.
authors.affiliation: Department of Chemistry, Institute of Nanosensor and Biotechnology, Dankook University, 126 Jukjeon-dong, Yongin-si, Gyeonggi-do 448-701, Republic of Korea.
authors.forename: Jihye
authors.initials: J
authors.lastname: Park
authors.forename: Aasif
authors.initials: A
authors.lastname: Helal
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Youngmi
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D007978:Levamisole / Q000494:pharmacology; D011522:Protons; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D011522:Protons; D007978:Levamisole; D000469:Alkaline Phosphatase