title: Measuring anisotropic cell motility on curved substrates.
abstract: Schwann cell motility was observed on laminin-coated quartz cylinders with different curvatures over an 18 hour period. A new analysis based on difference images helped to determine the minimal radius of curvature, 46 μm, which restricted motility along the cylinder axis. The migration speed, measured by calculating differences between successive images in the time series, ranged between 0.3 to 0.8 μm per minute and is similar to previously reported rates for Schwann cells. Difference images provide a rapid and simple method for the analysis of cell motility on large populations of cells.
authors.affiliation: CREOL, The College of Optics and Photonics, University of Central Florida, Orlando, FL 32816, USA.
authors.forename: Kyle M
authors.initials: KM
authors.lastname: Douglass
authors.forename: Nicklaus A
authors.initials: NA
authors.lastname: Sparrow
authors.forename: Marga
authors.initials: M
authors.lastname: Bott
authors.forename: Cristina
authors.initials: C
authors.lastname: Fernandez-Valle
authors.forename: Aristide
authors.initials: A
authors.lastname: Dogariu
mesh_terms: D000818:Animals; D016880:Anisotropy; D002448:Cell Adhesion; D002465:Cell Movement; D057054:Molecular Imaging; D051381:Rats; D012583:Schwann Cells / Q000166:cytology*