title: [The level of matrix metalloproteinases and type IV collagen in the gingival mucosa under different clinical forms of periodontitis in pre-and pubertal periods and their prognostic value].
abstract: The purpose of the study - the analysis of the prevalence and gravity of periodontal diseases among school adolescents and detection the relationship between the degree of periodontal tissues changes and the hormonal status of the prepubertal and pubertal periods.   The total number of observations - 618 Tbilisi schoolchildren at the age of 9 to 17 years. Cohort group (n=50) was divided by age: 1) 9-13 years (n=26), 2) 14-17 years (n=24).  The material for morphological and immunohistochemical study were the samples of periodontal tissues, received during the tooth extraction according the orthodontic testimony.  The samples of the gingival mucosa was stained by hematoxylin and eosin, in the same samples the Matrix Metalloproteinases expression and activity were determined by immunohistochemistry using a primary antibody MMP-1 and MMP-2 ("Novocastra", UK). The results of immunohistochemical reactions were estimated in scores by semiquantitative method. For the detection of type IV collagen in basement membranes the samples were stained by the "Direct Red-80" ("Sigma").  It was found that the increase of Matrix Metalloproteinases expression and the type IV collagen degeneration in basement membrane depend on the age, particularly the expression of MMP-1 and MMP-2 in the period of 14-17 years (puberty) is upwards than in the group of 9-13 years.  Studies have also demonstrated the clinical significance of Matrix Metalloproteinases ММР-1, 2 activity indicating the dissociation of the extracellular substance and type IV collagen in the assessment of severity and prognosis of periodontal complex pathology in pre-and pubertal periods.
authors.forename: Z G
authors.initials: ZG
authors.lastname: Tsagareli
authors.forename: T E
authors.initials: TE
authors.lastname: Shishniashvili
authors.forename: L E
authors.initials: LE
authors.lastname: Gogiashvili
authors.forename: T I
authors.initials: TI
authors.lastname: Kvachadze
authors.forename: N B
authors.initials: NB
authors.lastname: Khimshiashvili
mesh_terms: D000293:Adolescent; D002648:Child; D024141:Collagen Type IV / Q000378:metabolism*; D005260:Female; D005881:Gingiva / Q000378:metabolism / Q000473:pathology; D005891:Gingivitis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D009092:Mucous Membrane / Q000378:metabolism / Q000473:pathology; D010518:Periodontitis / Q000378:metabolism / Q000473:pathology*; D011237:Predictive Value of Tests; D014081:Tooth Extraction
chemical_list: D024141:Collagen Type IV; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1