title: The structure and spatio-temporal distribution of the Archaea in a horizontal subsurface flow constructed wetland.
abstract: In this study, archaeal community structure and temporal dynamics were monitored, using 16S rRNA clone libraries construction from a horizontal subsurface flow constructed wetland. Phylogenetic assignation of 1026 16S rRNA gene sequences shows that 96.2% of the total operational taxonomic units (OTUs) were affiliated with Thaumarchaeota, a newly proposed archaeal phylum and 3.7% with unclassified Archaea. Among the total sequences, 42% and 40.2% were affiliated with Candidatus Nitrososphaera and unclassified Nitrosopumilus respectively with more than 99% similarity. Results suggest that several dominant and active nitrifiers may benefit from the micro-aerobic conditions around the reed roots to perform ammonia oxidation. The archaeal diversity detected in the rhizosphere zone is clearly different from that detected in the bottom basin. This engineered habitat revealed the reed root and the water composition effects on the archaeal diversity.
authors.affiliation: Laboratoire d'Analyses, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie, rue Avicenne 5000 Monastir, Université de Monastir, Tunisia. geno.latvpep@gmail.com
authors.forename: Moez
authors.initials: M
authors.lastname: Bouali
authors.forename: Ines
authors.initials: I
authors.lastname: Zrafi-Nouira
authors.forename: Amina
authors.initials: A
authors.lastname: Bakhrouf
authors.forename: Denis
authors.initials: D
authors.lastname: Le Paslier
authors.forename: Sébastien
authors.initials: S
authors.lastname: Chaussonnerie
authors.forename: Emna
authors.initials: E
authors.lastname: Ammar
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Sghir
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D044822:Biodiversity; D019641:DNA, Archaeal / Q000235:genetics; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014416:Tunisia; D062065:Wastewater / Q000382:microbiology; D053833:Wetlands
chemical_list: D000641:Ammonia; D019641:DNA, Archaeal; D062065:Wastewater