title: Characteristics  of protein variants in trichlorphon-resistant Bactrocera dorsalis (Diptera;  Tephritidae) larvae.
abstract: Functional  proteins in larvae of Bactrocera dorsalis, a major fruit pest, play a  central role in their resistance to organophosphorus insecticides. Changes in  proteins in B. dorsalis larvae after trichlorphon treatment may have a  role in the resistance response to trichlorphon. We analyzed 14 protein spots  of crude proteins from B. dorsalis larvae post-treatment with trichlorphon  in two-dimensional gel electrophoresis through mass spectrometry and protein  sequencing. We found functional proteins that are responsible for signal  transduction (pkaap and dual specificity tyrosine-phosphorylation-regulated  kinase), immunity (hemolectin), synthesis and decomposition (twinstar,  cathepsin B, RE66325p), oxidative stress metabolism (glutathione S transferase  and CG7320), energy metabolism (Act57B), and cytoskeleton formation (actin).  These proteins appear to be involved in the resistance response to  trichlorphon.
authors.affiliation: Laboratory of Insect Ecology, College of Natural Resources and Enviornment, South China Agricultural University, Guangzhou, China.
authors.forename: T
authors.initials: T
authors.lastname: Jin
authors.forename: L
authors.initials: L
authors.lastname: Zeng
authors.forename: Y-Y
authors.initials: YY
authors.lastname: Lin
authors.forename: Y-Y
authors.initials: YY
authors.lastname: Lu
authors.forename: G-W
authors.initials: GW
authors.lastname: Liang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D019476:Insect Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D007305:Insecticide Resistance / Q000187:drug effects*; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D033621:Tephritidae / Q000187:drug effects* / Q000378:metabolism*; D014236:Trichlorfon / Q000494:pharmacology*
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D050505:Mutant Proteins; D014236:Trichlorfon