title: Genotyping Echinococcus granulosus from dogs from Western Iran.
abstract: Cystic echinococcosis is a zoonotic infection caused by the dog tapeworm, Echinococcus granulosus. In the present study, adults of E. granulosus (n=20) were collected from 71 dogs from Western Iran and were genetically characterized using DNA sequencing of the partial mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1). Consensus sequences were obtained for cox1 (366) and nad1 (471) genes. Phylogenetic analysis of concatenated nad1 and cox1 nucleotide sequence data was performed using Bayesian Inference approach. Overall, the dog isolates indicated nine different sequences in cox1 and seven in nad1 genes. Three genotypes (G1 [75%], G2 [10%] and G3 [15%]) were identified from the isolates. The G2 sequences indicated 100% homology with reference G2 sequence in both cox1 (Genbank accession number M84662) and nad1 (AJ237633) genes. G3 sequences showed 100% homology with G3 reference sequence in nad1 (AJ237633), but displayed two different cox1 profiles, each having 99% homology with reference G3 sequence (M84663). In the phylogenetic tree all of the isolates were grouped into a distinct cluster corresponding to the G1-G3 complex with relevant reference sequences. The presence of G1 genotype (sheep strain) of E. granulosus sensu stricto as dominant genotype in dogs is emphasized. To the best of our knowledge, this study established the first record of E. granulosus sensu stricto, G2 genotype in Iran.
authors.affiliation: Department of Laboratory Sciences, Islamic Azad University, Borujerd Branch, Borujerd, Iran.
authors.forename: Farzad
authors.initials: F
authors.lastname: Parsa
authors.forename: Majid
authors.initials: M
authors.lastname: Fasihi Harandi
authors.forename: Sima
authors.initials: S
authors.lastname: Rostami
authors.forename: Mitra
authors.initials: M
authors.lastname: Sharbatkhori
mesh_terms: D000818:Animals; D018509:DNA, Helminth / Q000737:chemistry; D004283:Dog Diseases / Q000469:parasitology* / Q000635:transmission; D004285:Dogs; D004443:Echinococcosis / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000145:classification* / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D005838:Genotype; D060005:Genotyping Techniques / Q000662:veterinary; D007492:Iran; D008297:Male; D009243:NAD / Q000235:genetics*
chemical_list: D018509:DNA, Helminth; D009243:NAD; D003576:Electron Transport Complex IV