title: Identification and characterization of lactic acid bacteria isolated from traditional pickles in Sichuan, China.
abstract: The pickle, a traditional fermented vegetable product, is popular in Sichuan Province of China. The objective of this study was to investigate the diversity of dominant lactic acid bacteria (LAB) in pickles by analyzing 36 samples collected from 6 different regions in Sichuan Province. The LAB counts in these samples varied from 3.90 to 8.40 log cfu ml(-1). In total, 185 presumptive LAB with Gram-positive and catalase-negative properties were obtained from these samples using MRS agar, and those strains were identified at the species level by physiological tests, 16S rRNA gene sequencing and multiplex PCR assay. The results revealed that all isolates were accurately identified as Enterococcus thailandicus (2 strains), Lactobacillus alimentarius (16 strains), L. brevis (24 strains), L. paracasei (9 strains), L. plantarum (81 strains), L. pentosus (38 strains), L. sakei (8 strains), L. spicheri (1 strain), Leuconostoc lactis (1 strain) and Pediococcus ethanolidurans (5 strains). The predominant LAB in Sichuan pickle was L. plantarum, which were isolated from most samples. The results also demonstrated that different regions in Sichuan Province have complex compositions of LAB species, and such a rich resource of LAB strains provides raw data for further studies involving probiotic strain selection.
authors.affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Education Ministry of PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Wa
authors.initials: W
authors.lastname: Gao
authors.forename: Manjun
authors.initials: M
authors.lastname: Qing
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Sun
authors.forename: Weihong
authors.initials: W
authors.lastname: Wang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.forename: Ting
authors.initials: T
authors.lastname: Sun
authors.forename: Hongmei
authors.initials: H
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Bai
authors.forename: Heping
authors.initials: H
authors.lastname: Zhang
mesh_terms: D058491:Bacterial Load; D015373:Bacterial Typing Techniques; D058448:Biota; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005516:Food Microbiology; D019344:Lactic Acid / Q000378:metabolism*; D056546:Lactobacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D019344:Lactic Acid