title: Amniotic membrane traps and induces apoptosis of inflammatory cells in ocular surface chemical burn.
abstract: OBJECTIVE
  Severe chemical burns can cause necrosis of ocular surface tissues following the infiltration of inflammatory cells. It has been shown that amniotic membrane transplantation (AMT) is an effective treatment for severe chemical burns, but the phenotypes of cells that infiltrate the amniotic membrane and the clinical significance of these cellular infiltrations have not previously been reported. The present work studies the inflammation cell traps and apoptosis inducing roles of the amniotic membrane after AMT in patients with acute chemical burns.
  
  
  METHODS
  A total of 30 patients with acute alkaline burns were classified as having either moderate or severe burns. In all participants, AMT was performed within one week of his/her injury. After 7-9 days, the transplanted amniotic membranes were removed. Histopathological and immunohistochemical techniques were used for the examination and detection of infiltrating cells, and tests for the expression of CD (cluster of differentiation)15, CD68, CD3, CD20, CD57, CD31, CD147, and CD95 (Fas) were performed. A TUNEL (TdT-mediated dUTP nick end labeling) assay was used to confirm apoptosis of the infiltrating cells. Three patients with herpes simplex-induced keratitis who had undergone AMT to treat persistent epithelium defects were used as a control group. Amniotic membrane before transplantation was used as another control.
  
  
  RESULTS
  After amniotic membrane transplantation, the number of infiltrating cells in patients with severe burns was significantly higher than in patients with moderate burns or in control patients (p<0.05). Among the severe burns patients, CD15 and CD68 were widely expressed in the infiltrating cells, and CD3, CD20, and CD57 were only found in a small number of cells. Occasionally, CD31-positive cells were found in the amniotic membranes. More cells that were CD147, Fas, and TUNEL positive were found in patients with severe burns than in patients with moderate burns or in control patients.
  
  
  CONCLUSIONS
  Neutrophils and macrophages were the main cells that had infiltrated into the amniotic membrane during the acute phase of healing from a chemical burns. AMT can trap different inflammatory cells and induce apoptosis of inflammatory cells in acute ocular chemical burns.
authors.affiliation: State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Hualei
authors.initials: H
authors.lastname: Zhai
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xu
authors.forename: Yanling
authors.initials: Y
authors.lastname: Dong
authors.forename: Yajie
authors.initials: Y
authors.lastname: Sun
authors.forename: Xinjie
authors.initials: X
authors.lastname: Zang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000468:Alkalies; D000650:Amnion / Q000637:transplantation*; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D017209:Apoptosis; D002057:Burns, Chemical; D002452:Cell Count; D002465:Cell Movement; D005126:Eye Burns / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D015870:Gene Expression; D006801:Humans; D020287:In Situ Nick-End Labeling; D007249:Inflammation / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D007634:Keratitis / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D016849:Keratitis, Herpetic / Q000378:metabolism / Q000473:pathology / Q000628:therapy / Q000821:virology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D009504:Neutrophils / Q000378:metabolism / Q000473:pathology; D012720:Severity of Illness Index; D016896:Treatment Outcome; D014945:Wound Healing
chemical_list: D000468:Alkalies; D015703:Antigens, CD