title: Agrin induces association of Chrna1 mRNA and nicotinic acetylcholine receptor in C2C12 myotubes.
abstract: In the mammalian central nervous system transcripts of certain synaptic components are localized near the synapse, allowing for rapid regulation of protein levels. Here we test whether an mRNA localization mechanism also exists in the postsynaptic specialization induced by agrin in C2C12 myotubes. RT-PCR showed that Chrna1 was co-purified with nicotinic acetylcholine receptor (AChR) isolated by affinity column or by ultracentrifugation. In addition, Stau1 was found to interact with Chrna1 mRNA, and knocking down of Stau1 by RNAi resulted in defective AChR clustering. These results suggest that mRNA localization also participates in the formation of mammalian neuromuscular junction (NMJ).
authors.affiliation: Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung City 807, Taiwan.
authors.forename: Yung-Fu
authors.initials: YF
authors.lastname: Chang
authors.forename: Hui-Ju
authors.initials: HJ
authors.lastname: Chou
authors.forename: Ying-Cheng
authors.initials: YC
authors.lastname: Yen
authors.forename: Hsueh-Wei
authors.initials: HW
authors.lastname: Chang
authors.forename: Yi-Ren
authors.initials: YR
authors.lastname: Hong
authors.forename: Hurng-Wern
authors.initials: HW
authors.lastname: Huang
authors.forename: Chao-Neng
authors.initials: CN
authors.lastname: Tseng
mesh_terms: D018171:Agrin / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D055785:Gene Knockdown Techniques; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000235:genetics* / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism
chemical_list: D018171:Agrin; C555576:CHRNA1 protein, mouse; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011978:Receptors, Nicotinic; C492187:Stau1 protein, mouse