title: Detection, isolation, and identification of Vibrio cholerae from the environment.
abstract: Recent molecular advances in microbiology have greatly improved the detection of bacterial pathogens in the environment. These improvements and a downward trend in the cost of molecular detection methods have contributed to increased frequency of detection of pathogenic microorganisms where traditional culture-based detection methods have failed. Culture methods also have been greatly improved, and the confluence of the two suites of methods provides a powerful tool for detection, isolation, and characterization of pathogens. While molecular detection provides data on the presence and type of pathogens, culturing methods allow a researcher to preserve the organism of interest for "-omics" studies, such as genomic, metabolomic, secretomic, and transcriptomic analysis, which are rapidly becoming more affordable. This has yielded a clearer understanding of the ecology and epidemiology of microorganisms that cause disease. In this unit, we present commonly accepted methods for isolation, detection, and characterization of V. cholerae, providing more extensive knowledge of the ecology and epidemiology of this organism. This unit has been fully revised and updated from the earlier version with the latest knowledge and additional information not previously included.
authors.affiliation: Maryland Pathogen Research Institute, Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, College Park, Maryland, USA.
authors.forename: Anwar
authors.initials: A
authors.lastname: Huq
authors.forename: Bradd J
authors.initials: BJ
authors.lastname: Haley
authors.forename: Elisa
authors.initials: E
authors.lastname: Taviani
authors.forename: Arlene
authors.initials: A
authors.lastname: Chen
authors.forename: Nur A
authors.initials: NA
authors.lastname: Hasan
authors.forename: Rita R
authors.initials: RR
authors.lastname: Colwell
mesh_terms: D000818:Animals; D002771:Cholera / Q000382:microbiology; D015169:Colony Count, Microbial / Q000379:methods*; D004783:Environmental Microbiology; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012758:Shellfish / Q000382:microbiology; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*