title: Identification of isomers and determination of octenylsuccinate in modified starch by HPLC and mass spectrometry.
abstract: A high performance liquid chromatographic (HPLC) analysis of octenylsuccinic anhydride (OSA) reagent and its bound and un-reacted forms in modified starch was developed. The HPLC system consisted of a C(18) column, an elution system of acetonitrile and water containing 0.1% TFA (45:55, v/v), and a diode array detector (DAD). Using the combination of HPLC-DAD and HPLC-APCI (atmospheric pressure chemical ionisation)-MS (mass spectrometry), four main and two minor peaks were identified as 1-OS acid, cis-2-OS acid, trans-2-OS acid, 1-OSA, cis-2-OSA and trans-2-OSA, respectively. Both free and bound OS content in modified starch were successfully determined by HPLC, and direct measurement of composition of OS was achieved.
authors.affiliation: School of Chemical Engineering, Ningbo University of Technology, 89, Cuibai Road, Ningbo, Zhejiang 315016, PR China. qiudan_zju@163.com
authors.forename: Dan
authors.initials: D
authors.lastname: Qiu
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Bai
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Shi
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007536:Isomerism; D013058:Mass Spectrometry / Q000379:methods*; D013213:Starch / Q000737:chemistry*; D013386:Succinates / Q000737:chemistry*
chemical_list: D013386:Succinates; C028480:octenyl succinate; D013213:Starch