title: Simultaneous decoloration and deproteinization of crude polysaccharide from pumpkin residues by cross-linked polystyrene macroporous resin.
abstract: A novel method for the purification of crude polysaccharide from fermentation broth of pumpkin residues by macroporous resins was developed. Through static adsorption and desorption and adsorption kinetic tests, six resins (AB-8, S-8, HPH480, HPD100, X-5, and D101) with different polarity, diameter, and surface area were studied for simultaneous decoloration and deproteinization of crude polysaccharide, and S-8 was chosen as the best one. Dynamic breakthrough and desorption tests were performed in a glass column packed with S-8 resin, and the resulting adsorption ratios of pigment and protein were 84.3% and 75.9% (w/w), respectively, with a recovery ratio of polysaccharide 84.7% (w/w). S-8 resin also exhibited higher purification efficiency than the other tested traditional methods. Moreover, UV/vis spectroscopy (200-900 nm) analysis revealed most of the pigment and protein were absorbed by S-8 resin, and HPLC (containing a refractive index detector and a HPSEC column) results indicated that there was no degradation of the polysaccharide. This automated and efficient method via adsorption-desorption strategy could have potential in scale-up purification and preparation of polysaccharide in the future.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University , National Engineering Research Center for Fruits and Vegetables Processing, Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Beijing 100083, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Demei
authors.initials: D
authors.lastname: Meng
authors.forename: Yi
authors.initials: Y
authors.lastname: Song
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Hu
authors.forename: Yuanying
authors.initials: Y
authors.lastname: Ni
authors.forename: Quanhong
authors.initials: Q
authors.lastname: Li
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry; D028464:Cucurbita / Q000737:chemistry*; D005285:Fermentation; D007700:Kinetics; D011134:Polysaccharides / Q000302:isolation & purification*; D011137:Polystyrenes; D012117:Resins, Synthetic / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D011134:Polysaccharides; D011137:Polystyrenes; D012117:Resins, Synthetic