title: Metabolic engineering of Escherichia coli for the production of 1-propanol.
abstract: An engineered Escherichia coli strain that produces 1-propanol under aerobic condition was developed based on an L-threonine-overproducing E. coli strain. First, a feedback resistant ilvA gene encoding threonine dehydratase was introduced and the competing metabolic pathway genes were deleted. Further engineering was performed by overexpressing the cimA gene encoding citramalate synthase and the ackA gene encoding acetate kinase A/propionate kinase II, introducing a modified adhE gene encoding an aerobically functional AdhE, and by deleting the rpoS gene encoding the stationary phase sigma factor. Fed-batch culture of the final engineered strain harboring pBRthrABC-tac-cimA-tac-ackA and pTacDA-tac-adhE(mut) allowed production of 10.8 g L(-1) of 1-propanol with the yield and productivity of 0.107 g g(-1) and 0.144 g L(-1) h(-1), respectively, from 100 g L(-1) of glucose, and 10.3 g L(-1) of 1-propanol with the yield and productivity of 0.259 g g(-1) and 0.083 g L(-1) h(-1), respectively, from 40 g L(-1) glycerol.
authors.affiliation: Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea.
authors.forename: Yong Jun
authors.initials: YJ
authors.lastname: Choi
authors.forename: Jin Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: Tae Yong
authors.initials: TY
authors.lastname: Kim
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
mesh_terms: D000433:1-Propanol / Q000378:metabolism*; D000084:Acetate Kinase / Q000235:genetics / Q000378:metabolism; D000332:Aerobiosis; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D060847:Metabolic Engineering; D007652:Oxo-Acid-Lyases / Q000235:genetics / Q000378:metabolism; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000235:genetics / Q000378:metabolism; D013913:Threonine Dehydratase / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D000433:1-Propanol; D017851:Phosphotransferases (Carboxyl Group Acceptor); C111066:TdcD protein, E coli; D000084:Acetate Kinase; D007652:Oxo-Acid-Lyases; C019907:citramalate lyase; D013913:Threonine Dehydratase