title: Association of vitamin D receptor gene polymorphisms with colon cancer.
abstract: OBJECTIVE
  In this study, we investigated the association of two vitamin D receptor (VDR) polymorphisms BsmI and TaqI with colon cancer in a Caucasian population.
  
  
  METHODS
  The VDR gene polymorphisms BsmI and TaqI were detected by polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP)-genotyping assays by using endonucleases BsmI and TaqI, and an agarose gel electrophoresis technique in a series of 43 colon cancer patients and 42 healthy controls.
  
  
  RESULTS
  Allele frequencies and genotype distributions were found to be similar in both cases and controls. When homozygous carriers and heterozygotes were combined for each allele, alleles B and T were found to be more common in the control group (p=0.039, χ(2)=4.276, odds ratio [OR]=0.312, 95% confidence interval [CI]=0.100-0.973 and p=0.039, χ(2)=4.258, OR=0.254, 95% CI=0.064-1.000, respectively). When genotypes were analyzed as pairs, the Bb/TT variant was higher in the control group at a statistically high significance (p=0.001, χ(2)=11.854, OR=0.122, 95% CI=0.032-0.460).
  
  
  CONCLUSIONS
  The alleles B and T and the genotype combination Bb/TT were found to be higher in the control group, and thus BsmI and TaqI polymorphisms of the VDR gene may be possible risk factors for colorectal carcinogenesis.
authors.affiliation: Department of General Surgery, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. drmucteba@hotmail.com
authors.forename: Mücteba
authors.initials: M
authors.lastname: Gündüz
authors.forename: Canan
authors.initials: C
authors.lastname: Cacına
authors.forename: Bahar
authors.initials: B
authors.lastname: Toptaş
authors.forename: İlhan
authors.initials: İ
authors.lastname: Yaylım-Eraltan
authors.forename: Yaman
authors.initials: Y
authors.lastname: Tekand
authors.forename: Turgay
authors.initials: T
authors.lastname: İsbir
mesh_terms: D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D018167:Receptors, Calcitriol / Q000235:genetics*; D014421:Turkey; D044465:White People / Q000235:genetics*
chemical_list: D018167:Receptors, Calcitriol; C053864:endodeoxyribonuclease BsmI; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases