title: [Pioglitazone, an activator of PPAR-gamma, reduces the expression of kB nuclear factor and inhibits apoptosis in mononuclear cells of peripheral blood in vitro].
abstract: In order to examine the impact of PPAR-gamma activator on the expression of kB nuclear transcription factor and apoptosis, the peripheral blood mononuclear cells (PBMC) were incubated with pioglitazone at concentrations 10, 30 and 100 MM. Expression of NF-kB in CD64+ cells was evaluated by flow cytometry using monoclonal antibodies. The development of apoptosis was evaluated using annexin V and propidium iodide. Activation of PPAR-y resulted in a reduction of the number of CD64+ cells and expression of the maximum levels of these receptors. We also obesrved a dose-dependent decrease of NF-kB concentration in PBMC suspension and in CD64+ cells. While analyzing the apoptosis, the reduction of apoptotic cells (reduction of AnV+PI+ cells) has been observed. The results obtained allow to prove that PPAR-gamma activation participates in the PBMC apoptosis regulation and in the expression of transcriptional factor NF-kB. Our results indicate for the involvement of PPAR-gamma in the regulation of NF-kB activity which plays one of the most significant roles in the inflammation realization, oncogenesis, and so on.
authors.forename: N L
authors.initials: NL
authors.lastname: Kutsenko
authors.forename: L E
authors.initials: LE
authors.lastname: Vesnina
authors.forename: I P
authors.initials: IP
authors.lastname: Kaĭdashev
mesh_terms: D000328:Adult; D017304:Annexin A5; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000235:genetics; D047495:PPAR gamma / Q000819:agonists* / Q000235:genetics; D000077205:Pioglitazone; D011419:Propidium; D017452:Receptors, IgG / Q000096:biosynthesis; D045162:Thiazolidinediones / Q000494:pharmacology*
chemical_list: D017304:Annexin A5; D007004:Hypoglycemic Agents; D016328:NF-kappa B; D047495:PPAR gamma; D017452:Receptors, IgG; D045162:Thiazolidinediones; D011419:Propidium; D000077205:Pioglitazone