title: [Influence of angiotensin-(1-7) on cell activation in rat renal interstitial fibroblasts induced by aldosterone].
abstract: OBJECTIVE
  To explore the influence of angiotensin-(1-7) [Ang-(1-7)] on cell  activation and extracellular matrix secretion in rat renal interstitial fibroblasts (NRK-49F) induced by aldosterone (ALD).
  
  
  METHODS
  The NRK-49F cells were cultured in vitro, and then were divided into control group, ALD group, Ang-(1-7) group, and ALD+Ang-(1-7) group. When the cells were cultured for 48 h, the expression of α-smooth muscle actin (α-SMA) (a sign of cell activation) was detected by immunocytochemistry; the level of collagen type  I (Col I ) in the cultured supernatant was measured by enzyme-linked immunosorbent assay (ELISA). When the cells were cultured for 30 min, the expressions of phosphorylated and total ERK1/2 (pERK1/2, tERK1/2) in the cell lysate were detected by Western blotting.
  
  
  RESULTS
  Compared with control group, the expressions of α-SMA, Col I  and the Phos/Total ERK1/2 ratio in ALD group and ALD+Ang-(1-7) group increased significantly (P<0.05). Compared with ALD group, the expressions of α-SMA, Col I  and the Phos/Total ERK1/2 ratio in ALD+Ang-(1-7) group decreased significantly (P<0.05).
  
  
  CONCLUSIONS
  Ang-(1-7) can inhibit ALD-induced cell activation and decrease the secretion of Col I  in rat renal interstitial fibroblasts. Inhibition of ERK1/2 pathway may play an important role in this process.
authors.affiliation: Department of Urology, Affiliated Hospital, Luzhou Medical College, Luzhou, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Tan
authors.forename: Ji-yi
authors.initials: JY
authors.lastname: Xia
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: He
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Jun-ming
authors.initials: JM
authors.lastname: Fan
authors.forename: San-tao
authors.initials: ST
authors.lastname: Ou
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
mesh_terms: D000199:Actins / Q000378:metabolism; D000450:Aldosterone / Q000494:pharmacology*; D000803:Angiotensin I / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D024042:Collagen Type I / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D007668:Kidney / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D012084:Renin-Angiotensin System
chemical_list: D000199:Actins; D000450:Aldosterone; D000803:Angiotensin I; D024042:Collagen Type I; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D010446:Peptide Fragments; C498862:Acta2 protein, rat; C118790:angiotensin I (1-7)