title: Solid-supported NOTA and DOTA chelators useful for the synthesis of 3'-radiometalated oligonucleotides.
abstract: Esterified precursors of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA; 18) and 1,4,7-triazacyclononane-1,4,7-trisacetic acid (NOTA; 17,19) ligands bearing a dimethoxytritylated hydroxyl side arm were prepared and immobilized via an ester linkage to long chain alkyl amine derivatized controlled pore glass (LCAA-CPG). Oligonucleotide chains were then assembled on the hydroxyl function and conjugates were released and deprotected by a two-step cleavage with aqueous alkali and ammonia. The 3'-DOTA and 3'-NOTA conjugated oligonucleotides were converted to (68)Ga chelates by a brief treatment with [(68)Ga]Cl(3) at elevated temperature. Applicability of the conjugates for in vivo imaging with positron emission tomography (PET) was verified.
authors.affiliation: Department of Chemistry, University of Turku, FI-20014 Turku, Finland.
authors.forename: Anu
authors.initials: A
authors.lastname: Kiviniemi
authors.forename: Joonas
authors.initials: J
authors.lastname: Mäkelä
authors.forename: Jussi
authors.initials: J
authors.lastname: Mäkilä
authors.forename: Tiina
authors.initials: T
authors.lastname: Saanijoki
authors.forename: Heidi
authors.initials: H
authors.lastname: Liljenbäck
authors.forename: Päivi
authors.initials: P
authors.lastname: Poijärvi-Virta
authors.forename: Harri
authors.initials: H
authors.lastname: Lönnberg
authors.forename: Tiina
authors.initials: T
authors.lastname: Laitala-Leinonen
authors.forename: Anne
authors.initials: A
authors.lastname: Roivainen
authors.forename: Pasi
authors.initials: P
authors.lastname: Virta
mesh_terms: D002614:Chelating Agents / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000737:chemistry*; D049268:Positron-Emission Tomography; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002614:Chelating Agents; D009841:Oligonucleotides