title: Effect of salinity on enzymatic activities in a submerged fixed bed biofilm reactor for municipal sewage treatment.
abstract: The effect of salinity on the hydrolytic enzymatic activities (acid phosphatase, alkaline phosphatase, glucosidase, protease and esterase) released by the microorganisms in a submerged fixed bed bioreactor for real urban wastewater treatment was investigated. The influence of salt (NaCl) on the enzymatic activities was evaluated in four different experiments with concentrations of NaCl of 0, 3.7, 24.1 and 44.1g/L, remaining constant all other operating parameters of the bioreactor. The results show that enzymatic activities were reduced when the salinity was increased in the influent and consequently the biotransformation of organic matter in the submerged fixed bed bioreactor significantly decreased. A redundancy analysis was performed to evaluate the relationships between enzymatic activities and physic-chemical parameters analyzed in the influent. According to the results obtained with the Monte Carlo permutation test, salinity and sampling day significantly contributed to explain the variation of enzymatic activities, showing a negative correlation.
authors.affiliation: Department of Microbiology, University of Granada, Granada, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Cortés-Lorenzo
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez-Díaz
authors.forename: C
authors.initials: C
authors.lastname: López-Lopez
authors.forename: M
authors.initials: M
authors.lastname: Sánchez-Peinado
authors.forename: B
authors.initials: B
authors.lastname: Rodelas
authors.forename: J
authors.initials: J
authors.lastname: González-López
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001419:Bacteria / Q000201:enzymology*; D018441:Biofilms / Q000187:drug effects*; D019149:Bioreactors; D001711:Biotransformation / Q000187:drug effects; D002947:Cities; D004305:Dose-Response Relationship, Drug; D004950:Esterases / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D008954:Models, Biological; D009010:Monte Carlo Method; D010447:Peptide Hydrolases / Q000378:metabolism; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D014865:Waste Disposal, Fluid / Q000379:methods*; D018508:Water Purification / Q000379:methods*
chemical_list: D012965:Sodium Chloride; D004950:Esterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005959:Glucosidases; D010447:Peptide Hydrolases