title: Magnetic affinity enzyme-linked immunoassay for diagnosis of Schistosomiasis japonicum in persons with low-intensity infection.
abstract: Most schistosome-endemic areas in China are characterized by low-intensity infections that are independent of prevalence. To establish an effective diagnostic method, we developed a magnetic affinity enzyme-linked immunoassay based on soluble egg antigens (SEA-MEIA) for diagnosing schistosomiasis in persons with low-intensity infection with Schistosoma japonicum by comparing it with a conventional enzyme-linked immunosorbent assay (ELISA). Our results showed that the SEA-MEIA had a higher sensitivity and greater precision in the diagnosis of low-intensity S. japonicum infections than the ELISA. In addition, when we used Pearson's correlation in associating SEA-MEIA with ELISA, a significant correlation existed between the two assays (r = 0.845, P < 0.001). Our data indicated that SEA-MEIA, with a higher sensitivity and greater ease of performance, would be valuable for diagnosis of schistosomiasis japonicum in persons with low-intensity infections.
authors.affiliation: Department of Biochemistry and Molecular Biology, Tongji Medical College, Wuhan, People's Republic of China. qinyu027@hotmail.com
authors.forename: Qin
authors.initials: Q
authors.lastname: Yu
authors.forename: Hai
authors.initials: H
authors.lastname: Yang
authors.forename: Youmei
authors.initials: Y
authors.lastname: Feng
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiangliang
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010063:Ovum / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis* / Q000503:physiopathology*; D012680:Sensitivity and Specificity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth