title: Immature CD4⁺ dendritic cells conditioned with donor kidney antigen prolong renal allograft survival in rats.
abstract: BACKGROUND
  Allogeneic transplant rejection is currently a major problem encountered during organ transplantation. The dendritic cell (DC) is the most effective powerful known professional antigen-presenting cell, and recent studies have found that DCs can also induce immune tolerance, and avoid or reduce the degree of transplant rejection. The aim of this study was to evaluate the effect of transfused immature CD4(+) DCs on renal allografts in the rat model.
  
  
  METHODS
  In this study, we induced CD4(+) immature DCs from rat bone marrow cells by a cytokine cocktail. The immature CD4(+) DCs were identified by morphological analysis and then the suppressive activity of these cells conditioned with donor kidney antigen was evaluated in vitro and in vivo.
  
  
  RESULTS
  Immature CD4(+) DCs conditioned with donor kidney antigen possessed immunosuppressive activity in vitro and they were able to prolong renal transplant survival in an allograft rat model in vivo.
  
  
  CONCLUSIONS
  Our study provides new information on efficacious renal transplantation, which might be useful for understanding the function of immature CD4(+) DCs in modulating renal transplant rejection and improving clinical outcome in future studies.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510630, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Huang
authors.forename: Sheng-Ping
authors.initials: SP
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Miao
authors.forename: Ning
authors.initials: N
authors.lastname: Na
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D015704:CD4 Antigens / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006085:Graft Survival; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014184:Transplantation, Homologous / Q000276:immunology*
chemical_list: D000941:Antigens; D015704:CD4 Antigens; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma