title: Increased susceptibility to Salmonella infection in signal regulatory protein α-deficient mice.
abstract: Recent studies have shed light on the connection between elevated erythropoetin production/spleen erythropoiesis and increased susceptibility to Salmonella infection. In this article, we provide another mouse model, the SIRPα-deficient (Sirpα⁻/⁻) mouse, that manifests increased erythropoiesis as well as heightened susceptibility to Salmonella infection. Sirpα⁻/⁻ mice succumbed to systemic infection with attenuated Salmonella, possessing significantly higher bacterial loads in both the spleen and the liver. Moreover, Salmonella-specific Ab production and Ag-specific CD4 T cells were reduced in Sirpα⁻/⁻ mice compared with wild-type controls. To further characterize the potential mechanism underlying SIRPα-dependent Ag-specific CD4 T cell priming, we demonstrate that lack of SIRPα expression on dendritic cells results in less efficient Ag processing and presentation in vitro. Collectively, these findings demonstrate an indispensable role of SIRPα for protective immunity to Salmonella infection.
authors.affiliation: Department of Anatomy, Physiology and Cell Biology, Center for Comparative Medicine, School of Veterinary Medicine, University of California, Davis, Davis, CA 95616, USA. lxli@ucdavis.edu
authors.forename: Lin-Xi
authors.initials: LX
authors.lastname: Li
authors.forename: Shaikh M
authors.initials: SM
authors.lastname: Atif
authors.forename: Shirdi E
authors.initials: SE
authors.lastname: Schmiel
authors.forename: Seung-Joo
authors.initials: SJ
authors.lastname: Lee
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: McSorley
mesh_terms: D000818:Animals; D002690:Chlamydia Infections / Q000235:genetics / Q000276:immunology / Q000473:pathology; D023502:Chlamydia muridarum / Q000276:immunology; D020022:Genetic Predisposition to Disease; D051379:Mice; D057507:Mice, 129 Strain; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011971:Receptors, Immunologic / Q000172:deficiency* / Q000235:genetics; D012480:Salmonella Infections / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D012486:Salmonella typhimurium / Q000276:immunology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology*
chemical_list: C501922:Sirpa protein, mouse; D011971:Receptors, Immunologic