title: A clinically-feasible protocol for using human platelet lysate and mesenchymal stem cells in regenerative therapies.
abstract: The transplantation of human stem cells seeded on biomaterials holds promise for many clinical applications in cranio-maxillo-facial tissue engineering and regenerative medicine. However, stem cell propagation necessary to produce sufficient cell numbers currently utilizes fetal calf serum (FCS) as a growth supplement which may subsequently transmit animal pathogens. Human platelet lysate (HPL) could potentially be utilized to produce clinical-grade stem cell-loaded biomaterials as an appropriate FCS substitute that is in line with clinically-applicable practice. The goal of this study was to investigate whether HPL can be successfully used to propagate human mesenchymal stem cells (HMSCs) seeded on clinically-approved collagen materials under clinically-applicable conditions using FCS as a control. HMSCs were isolated from bone marrow and cultured in the presence of 10% FCS or 10% HPL. Characterization of HMSCs was performed by flow cytometry and through osteogenic and adipogenic differentiation assays. Proliferative capacity of HMSCs on both matrices was investigated by mitochondrial dehydrogenase assays (WST) and tissue coverage scanning electron microscopy (SEM). The isolated HMSC differentiated into osteogenic and adipogenic cells authenticating the multipotentiality of the HMSCs. WST tests and the SEM images demonstrated that HPL was generally superior to FCS in promoting growth of seeded HMSCs. For all other tests HPL supported HMSCs at least equal to FCS. In conclusion, HPL is an effective growth factor to allow expansion of clinical-grade HMSCs on clinically-approved biomaterials for maxillofacial and oral implantology applications.
authors.affiliation: Clem Jones Research Centre For Stem Cells & Tissue Regenerative Therapies, Bond University, Gold Coast, QLD, Australia. pwarnke@bond.edu.au
authors.forename: Patrick H
authors.initials: PH
authors.lastname: Warnke
authors.forename: Andreas
authors.initials: A
authors.lastname: Humpe
authors.forename: Dirk
authors.initials: D
authors.lastname: Strunk
authors.forename: Sebastien
authors.initials: S
authors.lastname: Stephens
authors.forename: Frauke
authors.initials: F
authors.lastname: Warnke
authors.forename: Joerg
authors.initials: J
authors.lastname: Wiltfang
authors.forename: Katharina
authors.initials: K
authors.lastname: Schallmoser
authors.forename: Mohammad
authors.initials: M
authors.lastname: Alamein
authors.forename: Robert
authors.initials: R
authors.lastname: Bourke
authors.forename: Peter
authors.initials: P
authors.lastname: Heiner
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D001672:Biocompatible Materials / Q000737:chemistry; D001769:Blood; D001792:Blood Platelets / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D003094:Collagen / Q000737:chemistry; D005240:Feasibility Studies; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D005562:Formazans; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000627:therapeutic use*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D012038:Regeneration / Q000502:physiology*; D013778:Tetrazolium Salts; D013997:Time Factors; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D005456:Fluorescent Dyes; D005562:Formazans; D036341:Intercellular Signaling Peptides and Proteins; D013778:Tetrazolium Salts; C079782:MTT formazan; D003094:Collagen; D016650:Fluorescein-5-isothiocyanate