title: Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts.
abstract: Far-red and near-infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole-body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti-HER2/neu scFv antibodies. HER2/neu-positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor-targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD-4D5scFv in tumors was considerably stronger than that of QD-PEG.
authors.affiliation: NI Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia. irin-b@mail.ru
authors.forename: Irina V
authors.initials: IV
authors.lastname: Balalaeva
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Zdobnova
authors.forename: Irina V
authors.initials: IV
authors.lastname: Krutova
authors.forename: Anna A
authors.initials: AA
authors.lastname: Brilkina
authors.forename: Ekaterina N
authors.initials: EN
authors.lastname: Lebedenko
authors.forename: Sergey M
authors.initials: SM
authors.lastname: Deyev
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology*; D016466:CHO Cells; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D015335:Molecular Probes / Q000737:chemistry* / Q000378:metabolism*; D045663:Quantum Dots; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D051598:Whole Body Imaging / Q000379:methods*
chemical_list: D015335:Molecular Probes; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human