title: Bio-functionalized graphene-graphene oxide nanocomposite based electrochemical immunosensing.
abstract: We report a novel in-situ electrochemical synthesis approach for the formation of functionalized graphene-graphene oxide (fG-GO) nanocomposite on screen-printed electrodes (SPE). Electrochemically controlled nanocomposite film formation was studied by transmission electron microscopy (TEM) and Raman spectroscopy. Further insight into the nanocomposite has been accomplished by the Fourier transformed infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA) and X-ray diffraction (XRD) spectroscopy. Configured as a highly responsive screen-printed immunosensor, the fG-GO nanocomposite on SPE exhibits electrical and chemical synergies of the nano-hybrid functional construct by combining good electronic properties of functionalized graphene (fG) and the facile chemical functionality of graphene oxide (GO) for compatible bio-interface development using specific anti-diuron antibody. The enhanced electrical properties of nanocomposite biofilm demonstrated a significant increase in electrochemical signal response in a competitive inhibition immunoassay format for diuron detection, promising its potential applicability for ultra-sensitive detection of range of target analytes.
authors.affiliation: Institute of Microbial Technology (CSIR), Sector 39-A, Chandigarh 160036, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Sharma
authors.forename: Satish K
authors.initials: SK
authors.lastname: Tuteja
authors.forename: Vijayender
authors.initials: V
authors.lastname: Bhalla
authors.forename: G
authors.initials: G
authors.lastname: Shekhawat
authors.forename: Vinayak P
authors.initials: VP
authors.lastname: Dravid
authors.forename: C Raman
authors.initials: CR
authors.lastname: Suri
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation; D004237:Diuron / Q000032:analysis* / Q000276:immunology; D055664:Electrochemical Techniques / Q000295:instrumentation*; D006108:Graphite / Q000737:chemistry*; D006540:Herbicides / Q000032:analysis* / Q000276:immunology; D007118:Immunoassay / Q000295:instrumentation*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D006540:Herbicides; D010087:Oxides; D006108:Graphite; D004237:Diuron