title: Antiviral specificity of the Solea senegalensis Mx protein constitutively expressed in CHSE-214 cells.
abstract: Interferons play a key role in fish resistance to viral infections by inducing the expression of antiviral proteins, such as Mx. The aim of the present study was to test the antiviral activity of the Senegalese sole Mx protein (SsMx) against RNA and DNA viruses pathogenic to fish, i.e. the infectious pancreatic necrosis virus (IPNV, dsRNA), the viral haemorrhagic septicaemia virus (VHSV, ssRNA), and the European sheatfish virus (ESV, dsDNA), using a CHSE-214 cell clone expressing this antiviral protein. A strong inhibition of IPNV and VHSV replication was recorded in SsMx-expressing cells, as has been shown by the virus yield reduction and the decrease in the synthesis of the viral RNA encoding the polyprotein (for IPNV) and the nucleoprotein (for VHSV). The titres of these viruses replicating on SsMx-expressing cells were 100 times lower than those recorded on non-transfected cells. In contrast, SsMx did not inhibit ESV replication since no significant differences were observed regarding the virus yield or the major capsid protein gene transcription in transfected and non-transfected cells.
authors.affiliation: Department of Microbiology, Faculty of Sciences, University of Malaga, Campus Teatinos, 29071 Malaga, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Alvarez-Torres
authors.forename: Esther
authors.initials: E
authors.lastname: Garcia-Rosado
authors.forename: M Alejandra
authors.initials: MA
authors.lastname: Fernandez-Trujillo
authors.forename: Julia
authors.initials: J
authors.lastname: Bejar
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Alvarez
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Borrego
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Alonso
mesh_terms: D000818:Animals; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000096:biosynthesis; D005412:Flatfishes / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism / Q000494:pharmacology*; D018061:Infectious pancreatic necrosis virus / Q000187:drug effects*; D064547:Myxovirus Resistance Proteins; D029141:Novirhabdovirus / Q000187:drug effects*; D017995:Ranavirus / Q000187:drug effects*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012474:Salmon; D014162:Transfection; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D064547:Myxovirus Resistance Proteins; D019204:GTP-Binding Proteins