title: Comparison of in vitro hepatogenic differentiation potential between various placenta-derived stem cells and other adult stem cells as an alternative source of functional hepatocytes.
abstract: Mesenchymal stem cells (MSCs) are powerful sources for cell therapy in regenerative medicine. The capability to obtain effective stem cell-derived hepatocytes would improve cell therapy for liver diseases. Recently, various placenta-derived stem cells (PDSCs) depending on the localization of placenta have been suggested as alternative sources of stem cells are similar to bone marrow-derived MSC (BM-MSCs) and adipose-derived MSC (AD-MSCs). However, comparative studies for the potentials of the hepatogenic differentiation among various MSCs largely lacking. Therefore, we investigated to compare the potentials for hepatogenic differentiation of PDSCs with BM-MSCs, AD-MSCs, and UCB-MSCs. Several MSCs were isolated from human term placenta, adipose tissue, and umbilical cord blood and characterized isolated MSCs and BM-MSCs was performed by quantitative reverse transcription-PCR (RT-PCR) and special stains after mesodermal differentiation. The hepatogenic potential of PDSCs was compared with AD-MSCs, UCB-MSCs, and BM-MSCs using RT-PCR, PAS stain, ICG up-take assays, albumin expression, urea production, and cytokine assays. MSCs isolated from different tissues all presented similar characteristics of MSCs. However, the proliferative potential of PDSCs and the expression of hepatogenic markers in differentiated PDSCs were higher than other MSCs. Interestingly, the expression of hepatocyte growth factor (HGF) increased in PDSCs after hepatogenic differentiation. Interestingly, stem cell factor (SCF) expression in chorionic plate-derived MSCs, one of the PDSCs, was significantly higher than in the other PDSCs. Taken together, the results of the present study suggest that MSCs isolated from various adult tissues can be induced to undergo hepatogenic differentiation in vitro, and that PDSCs may have the greatest potential for hepatogenic differentiation and proliferation. Therefore, PDSCs could be used as a stem cell source for cell therapy in liver diseases.
authors.affiliation: CHA Placenta Institute, CHA University, Seoul, Republic of Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jieun
authors.initials: J
authors.lastname: Jung
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Cho
authors.forename: Chang Kyou
authors.initials: CK
authors.lastname: Lee
authors.forename: Seong-Gyu
authors.initials: SG
authors.lastname: Hwang
authors.forename: Gi Jin
authors.initials: GJ
authors.lastname: Kim
mesh_terms: D000273:Adipose Tissue / Q000254:growth & development; D053687:Adult Stem Cells / Q000166:cytology* / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D049109:Cell Proliferation; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D015870:Gene Expression; D017228:Hepatocyte Growth Factor / Q000235:genetics; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D019089:Stem Cell Factor / Q000235:genetics
chemical_list: D015415:Biomarkers; D019089:Stem Cell Factor; D017228:Hepatocyte Growth Factor