title: Optic atrophy 1 mediates mitochondria remodeling and dopaminergic neurodegeneration linked to complex I deficiency.
abstract: Mitochondrial complex I dysfunction has long been associated with Parkinson's disease (PD). Recent evidence suggests that mitochondrial involvement in PD may extend beyond a sole respiratory deficit and also include perturbations in mitochondrial fusion/fission or ultrastructure. Whether and how alterations in mitochondrial dynamics may relate to the known complex I defects in PD is unclear. Optic atrophy 1 (OPA1), a dynamin-related GTPase of the inner mitochondrial membrane, participates in mitochondrial fusion and apoptotic mitochondrial cristae remodeling. Here we show that complex I inhibition by parkinsonian neurotoxins leads to an oxidative-dependent disruption of OPA1 oligomeric complexes that normally keep mitochondrial cristae junctions tight. As a consequence, affected mitochondria exhibit major structural abnormalities, including cristae disintegration, loss of matrix density and swelling. These changes are not accompanied by mitochondrial fission but a mobilization of cytochrome c from cristae to intermembrane space, thereby lowering the threshold for activation of mitochondria-dependent apoptosis by cell death agonists in compromised neurons. All these pathogenic changes, including mitochondrial structural remodeling and dopaminergic neurodegeneration, are abrogated by OPA1 overexpression, both in vitro and in vivo. Our results identify OPA1 as molecular link between complex I deficiency and alterations in mitochondrial dynamics machinery and point to OPA1 as a novel therapeutic target for complex I cytopathies, such as PD.
authors.affiliation: Neurodegenerative Diseases Research Group, Vall d'Hebron Research Institute-CIBERNED, Barcelona, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Ramonet
authors.forename: C
authors.initials: C
authors.lastname: Perier
authors.forename: A
authors.initials: A
authors.lastname: Recasens
authors.forename: B
authors.initials: B
authors.lastname: Dehay
authors.forename: J
authors.initials: J
authors.lastname: Bové
authors.forename: V
authors.initials: V
authors.lastname: Costa
authors.forename: L
authors.initials: L
authors.lastname: Scorrano
authors.forename: M
authors.initials: M
authors.lastname: Vila
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology*; D019636:Neurodegenerative Diseases / Q000378:metabolism* / Q000473:pathology*; D029241:Optic Atrophy, Autosomal Dominant / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D045304:Cytochromes c; D004298:Dopamine