title: Osteoblast compatibility of materials depends on serum protein absorbability in osteogenesis.
abstract: Titanium has an osseointegrative property, while hydroxyapatite has an osteoconductive property. It remains a matter of controversy among researchers whether hydroxyapatite has higher osteoblast compatibility than titanium. Here, we compared the activities between osteoblasts cultured on titanium and those cultured on hydroxyapatite. An osteoblast-like cell line, MC3T3-E1, was cultured on machined titanium, evaporated titanium, and hydroxyapatite disks to compare the affinity of osteoblasts to each of these materials. The adhesion and proliferation of MC3T3-E1 cells were higher on hydroxyapatite disks than on the other disks. Osteoblast differentiation was not affected by the nature of disks investigated, but calcium was more easily deposited on the hydroxyapatite disks. The amount of absorbed serum proteins detected on hydroxyapatite was greater than that on titanium. In conclusion, our results indicate hydroxyapatite is a more suitable material for osteoblast growth than titanium because of its higher absorption of serum proteins.
authors.affiliation: Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Harimoto
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshihara
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagaoka
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Yoh-ichi
authors.initials: Y
authors.lastname: Tagawa
mesh_terms: D016475:3T3 Cells; D000042:Absorption; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D016348:Osseointegration / Q000502:physiology*; D010006:Osteoblasts / Q000502:physiology*; D014025:Titanium / Q000737:chemistry / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D001798:Blood Proteins; D017886:Durapatite; D014025:Titanium