title: Nestin expression is increased in the suprabasal epidermal layer in psoriasis vulgaris.
abstract: We investigated the expression of both epidermal fatty acid-binding protein (FABP5), a marker of transit amplifying cells, and nestin, a putative marker of epidermal stem cells, in psoriatic epidermis and in normal human cultured keratinocytes. In lesional psoriatic epidermis, immunostaining showed that the suprabasal layer was positive for nestin, with some cells co-expressing FABP5. Flow cytometric analysis revealed that the expression of both nestin and FABP5 were increased in keratinocytes cultured in a low concentration of calcium relative to those cultured in a high concentration of calcium. These results suggest that nestin and FABP5 are expressed in actively proliferating keratinocytes in vitro and in the suprabasal layer in lesional psoriatic epidermis, and that double-positive cells may identify transit amplifying cells in the epidermis.
authors.affiliation: Department of Dermatology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan. watarai@med.kitasato-u.ac.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Watarai
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Amoh
authors.forename: Hideki
authors.initials: H
authors.lastname: Maejima
authors.forename: Yuko
authors.initials: Y
authors.lastname: Hamada
authors.forename: Kensei
authors.initials: K
authors.lastname: Katsuoka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002122:Calcium Chloride; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D000078404:Epidermal Cells; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D011565:Psoriasis / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: C493412:FABP5 protein, human; D050556:Fatty Acid-Binding Proteins; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D002122:Calcium Chloride