title: Chromatin staining of Drosophila testes.
abstract: This protocol describes chromatin staining of Drosophila testes. To visualize DNA, preparations fixed using methanol-acetone, paraformaldehyde, or formaldehyde can be stained with several DNA-binding dyes. If the slides are to be examined with a fluorescence microscope equipped with filters that permit ultraviolet (UV) excitation, suitable dyes for DNA staining are Hoechst 33258 or 4',6-diamidino-2-phenylindole (DAPI). If the slides are to be analyzed with a confocal microscope not equipped with a UV laser, DNA can be stained with either propidium iodide or TOTO-3 iodide.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bonaccorsi
authors.forename: Maria G
authors.initials: MG
authors.lastname: Giansanti
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cenci
authors.forename: Maurizio
authors.initials: M
authors.lastname: Gatti
mesh_terms: D000818:Animals; D006690:Bisbenzimidazole / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D004396:Coloring Agents / Q000378:metabolism*; D003584:Cytological Techniques / Q000379:methods*; D004247:DNA / Q000378:metabolism; D004330:Drosophila / Q000166:cytology*; D004772:Entomology / Q000379:methods*; D007211:Indoles / Q000378:metabolism; D008297:Male; D011419:Propidium / Q000378:metabolism; D011804:Quinolines / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*; D013737:Testis / Q000166:cytology; D013844:Thiazoles / Q000378:metabolism
chemical_list: D002843:Chromatin; D004396:Coloring Agents; D007211:Indoles; D011804:Quinolines; C495966:TOTO-3 compound; D013844:Thiazoles; D011419:Propidium; C007293:DAPI; D004247:DNA; D006690:Bisbenzimidazole