title: Computational simulation of hemodynamic-driven growth and remodeling of embryonic atrioventricular valves.
abstract: Embryonic heart valves develop under continuous and demanding hemodynamic loading. The particular contributions of fluid pressure and shear tractions in valve morphogenesis are difficult to decouple experimentally. To better understand how fluid loads could direct valve formation, we developed a computational model of avian embryonic atrioventricular (AV) valve (cushion) growth and remodeling using experimentally derived parameters for the blood flow and the cushion stiffness. Through an iterative scheme, we first solved the fluid loads on the axisymmetric AV canal and cushion model geometry. We then applied the fluid loads to the cushion and integrated the evolution equations to determine the growth and remodeling. After a set time of growth, we updated the fluid domain to reflect the change in cushion geometry and resolved for the fluid forces. The rate of growth and remodeling was assumed to be a function of the difference between the current stress and an isotropic homeostatic stress state. The magnitude of the homeostatic stress modulated the rate of volume addition during the evolution. We found that the pressure distribution on the AV cushion was sufficient to generate leaflet-like elongation in the direction of flow, through inducing tissue resorption on the inflow side of cushion and expansion on the outflow side. Conversely, shear tractions minimally altered tissue volume, but regulated the remodeling of tissue near the cushion surface, particular at the leading edge. Significant shear and circumferential residual stresses developed as the cushion evolved. This model offers insight into how natural and perturbed mechanical environments may direct AV valvulogenesis and provides an initial framework on which to incorporate more mechano-biological details.
authors.affiliation: Department of Mechanical and Aerospace Engineering, Cornell University, 306 Weill Hall, Ithaca, NY 14853, USA. prb28@cornell.edu
authors.forename: Philip R
authors.initials: PR
authors.lastname: Buskohl
authors.forename: James T
authors.initials: JT
authors.lastname: Jenkins
authors.forename: Jonathan T
authors.initials: JT
authors.lastname: Butcher
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D003198:Computer Simulation; D047108:Embryonic Development / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology*; D006351:Heart Valves / Q000196:embryology* / Q000254:growth & development*; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology*; D008955:Models, Cardiovascular; D013314:Stress, Mechanical; D020257:Ventricular Remodeling