title: Molecular organization of various collagen fragments as revealed by atomic force microscopy and diffusion-ordered NMR spectroscopy.
abstract: Heterogeneous mixtures of collagen fragments can be used as nutrition supplement or as key ingredients for ointments with therapeutic relevance in wound healing. Some mixtures of collagen fragments are referred to as collagen hydrolysates owing to the production process with hydrolytic enzymes. Since the precise composition of collagen hydrolysates is generally unknown, it is of interest to analyze samples containing various collagen fragments with appropriate biophysical methods. Any product optimization without a profound knowledge concerning the size and the molecular weight distribution of its components is nearly impossible. It turned out that a combination of AFM methods with NMR techniques is exceptionally suited to examine the size range and the aggregation behavior of the collagen fragments in the hydrolysates of fish, jellyfish, chicken, porcine and bovine collagen. Supported by molecular modeling calculations, the AFM and NMR experiments provide a detailed knowledge about the composition of collagen hydrolysates and collagen ointments. Furthermore, the data allow a correlation between the size of the fragments and their potential bioactivity.
authors.affiliation: Institut für Biochemie und Endokrinologie, Veterinärmedizinische Fakultät, Justus-Liebig-Universität Gießen, Frankfurter Str. 100, 35392 Giessen, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Stötzel
authors.forename: Marloes
authors.initials: M
authors.lastname: Schurink
authors.forename: Hans
authors.initials: H
authors.lastname: Wienk
authors.forename: Uschi
authors.initials: U
authors.lastname: Siebler
authors.forename: Monika
authors.initials: M
authors.lastname: Burg-Roderfeld
authors.forename: Thomas
authors.initials: T
authors.lastname: Eckert
authors.forename: Bianca
authors.initials: B
authors.lastname: Kulik
authors.forename: Rainer
authors.initials: R
authors.lastname: Wechselberger
authors.forename: Judith
authors.initials: J
authors.lastname: Sewing
authors.forename: Jürgen
authors.initials: J
authors.lastname: Steinmeyer
authors.forename: Steffen
authors.initials: S
authors.lastname: Oesser
authors.forename: Rolf
authors.initials: R
authors.lastname: Boelens
authors.forename: Hans-Christian
authors.initials: HC
authors.lastname: Siebert
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D003094:Collagen / Q000737:chemistry* / Q000648:ultrastructure*; D006868:Hydrolysis; D018625:Microscopy, Atomic Force / Q000379:methods; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D037401:Scyphozoa; D013552:Swine
chemical_list: D003094:Collagen