title: CCL21 attenuates HSV-induced inflammation through up-regulation of CD8+ memory cells.
abstract: CCR7 and its ligand, CCL21, are known to establish microenvironments for the initiation of immune responses in secondary lymphoid tissue. It has also been reported that CCR7 ligand gene-deleted mice have defects in lymphocyte homing. In addition, the injection of the CCR7 ligand was shown to induce the expression of memory T cells. In this study, we analyzed the expression of CCR7 and its ligand in HSV-induced Behçet's disease (BD)-like inflammation of mice. Additionally, plasmids containing the CCR7 ligand CCL19 or CCL21, pcDNA3.1-CCL19 or pcDNA3.1-CCL21, respectively, were injected into symptomatic mice, and changes in the population of memory T cells were determined. After administration of pcDNA3.1-CCL21, the frequencies of CD8+CD44+, CD8+CD62L- memory T cells were significantly up-regulated and the symptoms were not deteriorated when compared to the control vector injected group. Specifically, the difference in frequencies of CCR7+ peripheral blood mononuclear cells between active BD patients and inactive BD patients was similar to that of HSV-induced BD-like mice. These results suggest that CCR7, its ligand, and CD8+ memory cells are correlated with the regulation of BD symptoms.
authors.affiliation: Laboratory of Cell Biology, Institute for Medical Sciences, Ajou University, Suwon, Republic of Korea.
authors.forename: Bunsoon
authors.initials: B
authors.lastname: Choi
authors.forename: Hyun Chul
authors.initials: HC
authors.lastname: Lim
authors.forename: Eun-So
authors.initials: ES
authors.lastname: Lee
authors.forename: A K M Mostafa
authors.initials: AK
authors.lastname: Anower
authors.forename: Seonghyang
authors.initials: S
authors.lastname: Sohn
mesh_terms: D000328:Adult; D000818:Animals; D001528:Behcet Syndrome / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D054421:Chemokine CCL21 / Q000235:genetics / Q000276:immunology*; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D005260:Female; D006561:Herpes Simplex / Q000235:genetics / Q000276:immunology* / Q000473:pathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D007249:Inflammation / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000821:virology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008875:Middle Aged; D054400:Receptors, CCR7 / Q000235:genetics / Q000276:immunology; D015854:Up-Regulation / Q000276:immunology*; D014709:Vero Cells
chemical_list: C515478:CCL21 protein, human; C515370:CCR7 protein, human; C515371:Ccr7 protein, mouse; D054421:Chemokine CCL21; D054400:Receptors, CCR7