title: Overexpression of chromatin assembly factor-1 p60, poly(ADP-ribose) polymerase 1 and nestin predicts metastasizing behaviour of oral cancer.
abstract: OBJECTIVE
  The natural history of oral squamous cell carcinomas (OSCCs) is variable and difficult to predict. This study aimed to assess the value of the expression of poly(ADP-ribose) polymerase 1 (PARP-1), chromatin assembly factor-1 (CAF-1)/p60 and the stem cell markers CD133, CD166, CD44, CD44v6 and nestin as markers of outcome and progression-free survival in OSCC patients.
  
  
  METHODS
  Clinical data were collected from 66 patients (41 male and 25 female, aged 29-92 years) who underwent surgery for OSCC of the tongue, floor, lips, and palate. During follow-up (range: 12-131 months), 14 patients experienced relapse/metastasis and/or death. The study was performed by immunohistochemistry on paraffin-embedded tumour tissues, western blot analysis of tumour protein lysates and human cell lines, and RNA silencing assays. In addition, the human papillomavirus (HPV) status of primary tumours was evaluated by immunohistochemistry and viral subtyping. Univariate and multivariate analyses were performed to determine the correlation between these parameters and the clinical and pathological variables of the study population.
  
  
  CONCLUSIONS
  We found that a PARP-1(high) /CAF-1 p60(high) /nestin(high) phenotype characterized the OSCCs with the worst prognosis (all HPV-negative). This may be of benefit in clinical management, since radio-enhancing anti-PARP-1 and/or anti-CAF-1/p60 agents may allow radioresistance to be bypassed in the nestin-overexpressing, metastasizing OSCC cells.
authors.affiliation: Department of Biomorphological and Functional Sciences, Pathology Section, School of Medicine, University 'Federico II', Naples, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Mascolo
authors.forename: Gennaro
authors.initials: G
authors.lastname: Ilardi
authors.forename: Maria Fiammetta
authors.initials: MF
authors.lastname: Romano
authors.forename: Angela
authors.initials: A
authors.lastname: Celetti
authors.forename: Maria
authors.initials: M
authors.lastname: Siano
authors.forename: Simona
authors.initials: S
authors.lastname: Romano
authors.forename: Chiara
authors.initials: C
authors.lastname: Luise
authors.forename: Francesco
authors.initials: F
authors.lastname: Merolla
authors.forename: Alba
authors.initials: A
authors.lastname: Rocco
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Vecchione
authors.forename: Gaetano
authors.initials: G
authors.lastname: De Rosa
authors.forename: Stefania
authors.initials: S
authors.lastname: Staibano
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism* / Q000401:mortality; D056504:Chromatin Assembly Factor-1 / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000401:mortality; D009362:Neoplasm Metastasis / Q000175:diagnosis*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D011237:Predictive Value of Tests; D011379:Prognosis; D015996:Survival Rate; D014157:Transcription Factors; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C489780:CNOT8 protein, human; D056504:Chromatin Assembly Factor-1; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D014157:Transcription Factors; D011065:Poly(ADP-ribose) Polymerases