title: [Evaluation of the intracellular expression of interleukin 17 in patients with ovarian cancer].
abstract: Knowledge of the role of interleukin 17 (IL-17) has led to the identification of new subpopulation of T helper lymphocytes--Th17 and T cytotoxic lymphocytes secreting IL-17 (Tc17). An increasing amount of attention is paid to their role in anti-tumor immunity.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the percentage of peripheral blood, peritoneal fluid and cancer tissue CD4+ and CD8+ T lymphocytes producing IL-17 in patients with ovarian cancer
  
  
  METHODS
  Forty patients operated due to advanced ovarian carcinoma and twenty-four patients with functional follicle ovarian cysts were recruited. Peripheral blood, peritoneal fluid and cancer tissue mononuclear cells from ovarian cancer patients were stimulated for 4 hours ex vivo with phorbol myristate acetate (PMA) (50 ng/ ml) and ionomycin (1 microg/ml). The percentage of CD4+ and CD8+ T cells producing IL-17 was measured using flow cytometry.
  
  
  RESULTS
  CD4+ and CD8+ T lymphocytes producing IL-17 were detected in the peripheral blood, peritoneal fluid and cancer tissue of ovarian cancer patients. The percentage of CD4+ T cells producing IL-17 was higher in the peripheral blood, peritoneal fluid and cancer tissue when compared to CD8+/IL 17+ T cells. The percentage of CD4+/ IL-17+ was significantly higher in cancer tissue compared to T cells derived form peripheral blood. There was no difference in the percentage of CD4+/IL-17 + T cells between peripheral blood and peritoneal fluid and peritoneal fluid and cancer tissue of ovarian cancer patients. There was no difference in the percentage of CD8+/IL-17 + T lymphocytes in the peripheral blood, peritoneal fluid and cancer tissue in patients suffering from ovarian cancer.
  
  
  CONCLUSIONS
  Increased percentage of CD4+/IL-17+ and CD8+/IL-17+ T cells in cancer tissue indicates that these cells are accumulated in ovarian cancer microenvironment.
authors.affiliation: I Katedra i Klinika Ginekologii Onkologicznej i Ginekologii Uniwersytetu Medycznego w Lublinie, Polska. evelin84@wp.pl
authors.forename: Ewelina
authors.initials: E
authors.lastname: Rogala
authors.forename: Aldona
authors.initials: A
authors.lastname: Nowicka
authors.forename: Wiesława
authors.initials: W
authors.lastname: Bednarek
authors.forename: Bartłomiej
authors.initials: B
authors.lastname: Barczyński
authors.forename: Iwona
authors.initials: I
authors.lastname: Wertel
authors.forename: Maciej
authors.initials: M
authors.lastname: Zakrzewski
authors.forename: Jan
authors.initials: J
authors.lastname: Kotarski
mesh_terms: D001202:Ascitic Fluid / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000097:blood / Q000276:immunology* / Q000473:pathology; D011044:Poland; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D020381:Interleukin-17