title: Microbiological characterization in children with aggressive periodontitis.
abstract: The objective of this study was to characterize the subgingival microbiota of African-American children with Localized Aggressive Periodontitis (LAP). Fifty-one children were included. Subgingival plaque samples were taken from diseased (DD) and healthy sites (DH) in LAP and from healthy sites in HS and HC and analyzed by 16S rRNA-based microarrays. Aggregatibacter actinomycetemcomitans (Aa) was the only species found to be both more prevalent (OR = 8.3, p = 0.0025) and abundant (p < 0.01) in DD. Filifactor alocis (Fa) was also found to be more prevalent in DD (OR 2.31, CI 1.06-5.01, p = 0.03). Most prevalent species in healthy sites were Selenomonas spp, Veillonella spp, Streptococcus spp, Bergeyella sp, and Kingella oralis. Overall, Streptococcus spp, Campylobacter gracilis, Capnocytophaga granulosa, Haemophilus parainfluenzae, and Lautropia mirabilis were most abundant in healthy children, while Aa, Fa, Tannerella sp, Solobacterium moorei, Parvimonas micra, and Capnocytophaga sp were most abundant in LAP. Based on a comprehensive analysis with 16S rRNA-based microarrays, Aa was strongly associated and site-specific in LAP. In contrast, other species were found to be associated with healthy sites and individuals (ClinicalTrials.gov number CT01330719).
  
  
  BACKGROUND
  healthy site in healthy sibling (HS); healthy site in healthy control child (HC).
authors.affiliation: Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL, USA. lshaddox@dental.ufl.edu
authors.forename: L M
authors.initials: LM
authors.lastname: Shaddox
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.forename: T
authors.initials: T
authors.lastname: Lin
authors.forename: W
authors.initials: W
authors.lastname: Hou
authors.forename: P L
authors.initials: PL
authors.lastname: Harrison
authors.forename: I
authors.initials: I
authors.lastname: Aukhil
authors.forename: C B
authors.initials: CB
authors.lastname: Walker
authors.forename: V
authors.initials: V
authors.lastname: Klepac-Ceraj
authors.forename: B J
authors.initials: BJ
authors.lastname: Paster
mesh_terms: D000293:Adolescent; D001741:Black or African American; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000302:isolation & purification*; D010520:Aggressive Periodontitis / Q000382:microbiology*; D000704:Analysis of Variance; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000235:genetics; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D005431:Florida; D006801:Humans; D016015:Logistic Models; D008297:Male; D010512:Periodontal Index; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial