title: Doxycycline-regulated  growth hormone gene expression system for swine.
abstract: Although  growth hormone gene transgenic animals are much larger than normal animals,  they manifest differences that have adverse effects on survival due to  overexpression of growth hormone. We developed a stable pig embryonic  fibroblast cell line expressing pig growth hormone (pGH) using the Tet-On  system, with which we can conditionally manipulate expression of pGH in vivo.  Inducible expression of pGH was achieved by combining reserve Tet-controlled  transcriptional activator and tetracycline-responsive element in a single  plasmid. The mRNA expression of pGH was significantly increased compared to the  non-induced group by about 10-fold. The controlled secretion of pGH induced by  doxycycline was further tested in stably transfected cells. We conclude that  inducible GH expression can be achieved in pig embryonic fibroblasts.
authors.affiliation: Department of Animal Breeding & Genetics, College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: B C
authors.initials: BC
authors.lastname: Jiang
authors.forename: D B
authors.initials: DB
authors.lastname: Yu
authors.forename: L J
authors.initials: LJ
authors.lastname: Wang
authors.forename: F L
authors.initials: FL
authors.lastname: Dong
authors.forename: H A
authors.initials: HA
authors.lastname: Kaleri
authors.forename: X G
authors.initials: XG
authors.lastname: Wang
authors.forename: N
authors.initials: N
authors.lastname: Ally
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: H L
authors.initials: HL
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D033481:Chromosomes, Mammalian / Q000235:genetics; D004318:Doxycycline / Q000494:pharmacology*; D004351:Drug Resistance / Q000187:drug effects; D004622:Embryo, Mammalian / Q000166:cytology; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D005822:Genetic Vectors / Q000235:genetics; D013006:Growth Hormone / Q000235:genetics* / Q000378:metabolism; D007621:Karyotyping; D016086:Lentivirus / Q000187:drug effects / Q000235:genetics; D010957:Plasmids / Q000378:metabolism; D011760:Pyrrolidinones / Q000494:pharmacology; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D034421:Sus scrofa / Q000235:genetics*; D019076:Transgenes / Q000235:genetics
chemical_list: D011760:Pyrrolidinones; C420726:blasticidin A; D013006:Growth Hormone; D004318:Doxycycline