title: pH neutralization protects against reduction in replicative lifespan following chronological aging in yeast.
abstract: Chronological and replicative aging have been studied in yeast as alternative paradigms for post-mitotic and mitotic aging, respectively. It has been known for more than a decade that cells of the S288C background aged chronologically in rich medium have reduced replicative lifespan relative to chronologically young cells. Here we report replication of this observation in the diploid BY4743 strain background. We further show that the reduction in replicative lifespan from chronological aging is accelerated when cells are chronologically aged under standard conditions in synthetic complete medium rather than rich medium. The loss of replicative potential with chronological age is attenuated by buffering the pH of the chronological aging medium to 6.0, an intervention that we have previously shown can extend chronological lifespan. These data demonstrate that extracellular acidification of the culture medium can cause intracellular damage in the chronologically aging population that is asymmetrically segregated by the mother cell to limit subsequent replicative lifespan.
authors.affiliation: Department of Pathology, University of Washington; Seattle, WA, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Murakami
authors.forename: Joe R
authors.initials: JR
authors.lastname: Delaney
authors.forename: Annie
authors.initials: A
authors.lastname: Chou
authors.forename: Daniel
authors.initials: D
authors.lastname: Carr
authors.forename: Jennifer
authors.initials: J
authors.lastname: Schleit
authors.forename: George L
authors.initials: GL
authors.lastname: Sutphin
authors.forename: Elroy H
authors.initials: EH
authors.lastname: An
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Castanza
authors.forename: Marissa
authors.initials: M
authors.lastname: Fletcher
authors.forename: Sarani
authors.initials: S
authors.lastname: Goswami
authors.forename: Sean
authors.initials: S
authors.lastname: Higgins
authors.forename: Mollie
authors.initials: M
authors.lastname: Holmberg
authors.forename: Jessica
authors.initials: J
authors.lastname: Hui
authors.forename: Monika
authors.initials: M
authors.lastname: Jelic
authors.forename: Ki-Soo
authors.initials: KS
authors.lastname: Jeong
authors.forename: Jin R
authors.initials: JR
authors.lastname: Kim
authors.forename: Shannon
authors.initials: S
authors.lastname: Klum
authors.forename: Eric
authors.initials: E
authors.lastname: Liao
authors.forename: Michael S
authors.initials: MS
authors.lastname: Lin
authors.forename: Winston
authors.initials: W
authors.lastname: Lo
authors.forename: Hillary
authors.initials: H
authors.lastname: Miller
authors.forename: Richard
authors.initials: R
authors.lastname: Moller
authors.forename: Zhao J
authors.initials: ZJ
authors.lastname: Peng
authors.forename: Tom
authors.initials: T
authors.lastname: Pollard
authors.forename: Prarthana
authors.initials: P
authors.lastname: Pradeep
authors.forename: Dillon
authors.initials: D
authors.lastname: Pruett
authors.forename: Dilreet
authors.initials: D
authors.lastname: Rai
authors.forename: Vanessa
authors.initials: V
authors.lastname: Ros
authors.forename: Alex
authors.initials: A
authors.lastname: Schuster
authors.forename: Minnie
authors.initials: M
authors.lastname: Singh
authors.forename: Benjamin L
authors.initials: BL
authors.lastname: Spector
authors.forename: Helen
authors.initials: H
authors.lastname: Vander Wende
authors.forename: Adrienne M
authors.initials: AM
authors.lastname: Wang
authors.forename: Brian M
authors.initials: BM
authors.lastname: Wasko
authors.forename: Brady
authors.initials: B
authors.lastname: Olsen
authors.forename: Matt
authors.initials: M
authors.lastname: Kaeberlein
mesh_terms: D000143:Acids / Q000378:metabolism; D002021:Buffers; D002453:Cell Cycle; D003470:Culture Media / Q000378:metabolism; D004261:DNA Replication; D005434:Flow Cytometry; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability; D008928:Mitochondria / Q000378:metabolism / Q000502:physiology; D008938:Mitosis; D009930:Organic Chemicals / Q000378:metabolism; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
chemical_list: D000143:Acids; D002021:Buffers; D003470:Culture Media; D009930:Organic Chemicals; C402795:SYTOX Green; D029701:Saccharomyces cerevisiae Proteins