title: Catalytic properties and amino acid sequence of endo-1→3-β-D-glucanase from the marine mollusk Tapes literata.
abstract: A specific 1→3-β-D-glucanase with molecular mass 37 kDa was isolated in homogeneous state from crystalline style of the commercial marine mollusk Tapes literata. It exhibits maximal activity within the pH range from 4.5 to 7.5 at 45°C. The 1→3-β-D-glucanase catalyzes hydrolysis of β-1→3 bonds in glucans as an endoenzyme with retention of bond configuration, and it has transglycosylating activity. The K(m) for hydrolysis of laminaran is 0.25 mg/ml. The enzyme is classified as a glucan endo-(1→3)-β-D-glucosidase (EC 3.2.1.39). The cDNA encoding this 1→3-β-D-glucanase from T. literata was sequenced, and the amino acid sequence of the enzyme was determined. The endo-1→3-β-D-glucanase from T. literata was assigned to the 16th structural family (GHF 16) of O-glycoside hydrolases.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, pr. 100 let Vladivostoku 159, 690022 Vladivostok, Russia. rarf@yandex.ru
authors.forename: A M
authors.initials: AM
authors.lastname: Zakharenko
authors.forename: M I
authors.initials: MI
authors.lastname: Kusaykin
authors.forename: S N
authors.initials: SN
authors.lastname: Kovalchuk
authors.forename: V V
authors.initials: VV
authors.lastname: Sova
authors.forename: A S
authors.initials: AS
authors.lastname: Silchenko
authors.forename: A A
authors.initials: AA
authors.lastname: Belik
authors.forename: S D
authors.initials: SD
authors.lastname: Anastyuk
authors.forename: Bui Minh
authors.initials: BM
authors.lastname: Ly
authors.forename: V A
authors.initials: VA
authors.lastname: Rasskazov
authors.forename: T N
authors.initials: TN
authors.lastname: Zvyagintseva
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D055162:Biocatalysis; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D005936:Glucans / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008974:Mollusca / Q000201:enzymology* / Q000378:metabolism; D055550:Protein Stability; D013696:Temperature
chemical_list: D005936:Glucans; D004693:Glucan Endo-1,3-beta-D-Glucosidase