title: Peptidome analysis of cerebrospinal fluid by LC-MALDI MS.
abstract: We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000-4,000 peptide-like features. Out of these, 730 peptides were identified by MS/MS. The majority of these peptides have not been previously reported in CSF. The identified peptides were found to originate from 104 proteins, of which several have been reported to be involved in different disorders of the central nervous system. These results support the notion that CSF peptidomics may be viable complement to proteomics in the search of biomarkers of CNS disorders.
authors.affiliation: Clinical Neurochemistry Laboratory, Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.
authors.forename: Mikko
authors.initials: M
authors.lastname: Hölttä
authors.forename: Henrik
authors.initials: H
authors.lastname: Zetterberg
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Mirgorodskaya
authors.forename: Niklas
authors.initials: N
authors.lastname: Mattsson
authors.forename: Kaj
authors.initials: K
authors.lastname: Blennow
authors.forename: Johan
authors.initials: J
authors.lastname: Gobom
mesh_terms: D000595:Amino Acid Sequence; D002556:Cerebrospinal Fluid Proteins / Q000737:chemistry / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D030562:Databases, Protein; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000134:cerebrospinal fluid* / Q000737:chemistry; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D002556:Cerebrospinal Fluid Proteins; D010455:Peptides; D020543:Proteome