title: Acceleration of deposition of Aβ(1-40) peptide on ultrasonically formed Aβ(1-42) nucleus studied by wireless quartz-crystal-microbalance biosensor.
abstract: High-frequency (~ 55 MHz) wireless quartz-crystal microbalance biosensor was used for studying heterogeneous deposition behavior of Aβ(1-40) peptide on Aβ(1-42) nuclei, which were grown under the stirring agitation and 200-kHz ultrasonication at pH 2.2, 4.6, and 7.4. The deposition reaction was monitored over 40 h, and the deposition rate was deduced. Among the agitation nuclei, the maximum deposition rate was observed on the nucleus grown at pH 4.6. However, ultrasonication nucleus grown at pH 7.4 produced much larger deposition rate, despite the same β-sheet concentration. This result indicates that local structural modulation is caused in the nucleus by ultrasonication, which adsorbs the Aβ peptide more actively than other nuclei. The resultant deposits clearly show oligomeric structure.
authors.affiliation: Graduate School of Engineering Science, Osaka University, Machikaneyama 1-3, Toyonaka, Osaka 560-8531, Japan. ogi@me.es.osaka-u.ac.jp
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Ogi
authors.forename: Masahiko
authors.initials: M
authors.lastname: Fukushima
authors.forename: Kentaro
authors.initials: K
authors.lastname: Uesugi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Yagi
authors.forename: Yuji
authors.initials: Y
authors.lastname: Goto
authors.forename: Masahiko
authors.initials: M
authors.lastname: Hirao
mesh_terms: D000327:Adsorption; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000528:radiation effects; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D010446:Peptide Fragments / Q000737:chemistry* / Q000528:radiation effects; D025941:Protein Interaction Mapping / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013010:Sonication / Q000295:instrumentation*
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42)