title: Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy.
abstract: C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
authors.forename: Shizuka
authors.initials: S
authors.lastname: Nakayama
authors.forename: Yiling
authors.initials: Y
authors.lastname: Luo
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: T Kwaku
authors.initials: TK
authors.lastname: Dayie
authors.forename: Herman O
authors.initials: HO
authors.lastname: Sintim
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000032:analysis; D018749:RNA, Plant / Q000737:chemistry; D058928:Riboswitch; D013050:Spectrometry, Fluorescence
chemical_list: D052157:Aptamers, Nucleotide; D018749:RNA, Plant; D058928:Riboswitch; C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP