title: OsWRKY30 is activated by MAP kinases to confer drought tolerance in rice.
abstract: Both the WRKY transcription factor (TF) and MAP kinases have been shown to regulate gene expression in response to biotic and abiotic stresses in plants. Several reports have shown that WRKY TFs may function downstream of MAPK cascades. Here, we have shown that OsWRKY30 interacted with OsMPK3, OsMPK4, OsMPK7, OsMPK14, OsMPK20-4, and OsMPK20-5, and could be phosphorylated by OsMPK3, OsMPK7, and OsMPK14. Overexpression of OsWRKY30 in rice dramatically increased drought tolerance. Overexpression of OsWRKY30AA, in which all SP (serine residue followed by proline residue) sites were replaced by AP (A, alanine), resulted in no improvement in drought tolerance. In addition, the function of transcriptional activation of OsWRKY30 was impaired after SP was replaced by AP. These results proved that the phosphorylation of OsWRKY30 by MAPKs was crucial in order for OsWRKY30 to perform its biological function.
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, People's Republic of China.
authors.forename: Huaishun
authors.initials: H
authors.lastname: Shen
authors.forename: Citao
authors.initials: C
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiuping
authors.initials: X
authors.lastname: Meng
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Chengcai
authors.initials: C
authors.lastname: Chu
authors.forename: Xiping
authors.initials: X
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics*; D019943:Amino Acid Substitution; D055864:Droughts; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009154:Mutation; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D010766:Phosphorylation; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D025941:Protein Interaction Mapping; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D020798:Two-Hybrid System Techniques
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid; D020928:Mitogen-Activated Protein Kinases