title: Isolation and identification of two novel umami and umami-enhancing peptides from peanut hydrolysate by consecutive chromatography and MALDI-TOF/TOF MS.
abstract: Peanut hydrolysate produced by crude protease extract from Aspergillus oryzae HN 3.042 was found to elicit intense umami and umami-enhancing effect. Taste profiles, amino acid and organic acid composition of peanut hydrolysate and its separation fractions by ultrafiltration were evaluated. The results revealed that peanut hydrolysate was mainly low molecular weight compounds. Fractions of 1-3 kDa and below 1 kDa prominently contributed to the umami taste and umami-enhancing effect of the peanut hydrolysate. The two fractions were further purified, using gel filtration chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC), in combination with sensory evaluation, to obtain a umami peptide and umami-enhancing peptide. The active peptides were identified as Ser-Ser-Arg-Asn-Glu-Gln-Ser-Arg (SSRNEQSR, 963.9 Da) and Glu-Gly-Ser-Glu-Ala-Pro-Asp-Gly-Ser-Ser-Arg (EGSEAPDGSSR, 1091.1 Da), by MALDI-TOF/TOF MS, respectively.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Guowan
authors.initials: G
authors.lastname: Su
authors.forename: Chun
authors.initials: C
authors.lastname: Cui
authors.forename: Lin
authors.initials: L
authors.lastname: Zheng
authors.forename: Bao
authors.initials: B
authors.lastname: Yang
authors.forename: Jiaoyan
authors.initials: J
authors.lastname: Ren
authors.forename: Mouming
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D010367:Arachis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005421:Flavoring Agents / Q000737:chemistry* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000302:isolation & purification; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D005421:Flavoring Agents; D010455:Peptides; D010936:Plant Extracts; D011492:Protein Hydrolysates