title: Expression, purification, crystallization and preliminary X-ray analysis of 4-hydroxy-3-methyl-2-keto-pentanoate aldolase (asHPAL) from Arthrobacter simplex strain AKU 626.
abstract: 4-Hydroxy-3-methyl-2-keto-pentanoate aldolase (asHPAL), an enzyme used in the synthesis of (2S,3R,4S)-4-hydroxyisoleucine, was crystallized in the absence and the presence of 2-ketobutyrate as one of its substrates by the sitting-drop vapour-diffusion method using PEG 400 as a precipitant. Crystals of asHPAL grown without and with 2-ketobutyrate diffracted to 1.60 and 1.55 Å resolution and belonged to space group C2, with unit-cell parameters a = 116.8, b = 88.2, c = 85.3 Å, β = 122.3° and a = 116.2, b = 88.1, c = 85.0 Å, β = 122.3°, respectively.
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Linjun
authors.initials: L
authors.lastname: Guo
authors.forename: Masahiko
authors.initials: M
authors.lastname: Okai
authors.forename: Tomoko
authors.initials: T
authors.lastname: Mase
authors.forename: Fabiana Lica
authors.initials: FL
authors.lastname: Imai
authors.forename: Takuya
authors.initials: T
authors.lastname: Miyakawa
authors.forename: Koji
authors.initials: K
authors.lastname: Nagata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamanaka
authors.forename: Hidemi
authors.initials: H
authors.lastname: Fujii
authors.forename: Makoto
authors.initials: M
authors.lastname: Hibi
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
mesh_terms: D001173:Arthrobacter / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry* / Q000302:isolation & purification; D015870:Gene Expression
chemical_list: D005634:Fructose-Bisphosphate Aldolase