title: Minimum requirements of flagellation and motility for infection of Agrobacterium sp. strain H13-3 by flagellotropic bacteriophage 7-7-1.
abstract: The flagellotropic phage 7-7-1 specifically adsorbs to Agrobacterium sp. strain H13-3 (formerly Rhizobium lupini H13-3) flagella for efficient host infection. The Agrobacterium sp. H13-3 flagellum is complex and consists of three flagellin proteins: the primary flagellin FlaA, which is essential for motility, and the secondary flagellins FlaB and FlaD, which have minor functions in motility. Using quantitative infectivity assays, we showed that absence of FlaD had no effect on phage infection, while absence of FlaB resulted in a 2.5-fold increase in infectivity. A flaA deletion strain, which produces straight and severely truncated flagella, experienced a significantly reduced infectivity, similar to that of a flaB flaD strain, which produces a low number of straight flagella. A strain lacking all three flagellin genes is phage resistant. In addition to flagellation, flagellar rotation is required for infection. A strain that is nonmotile due to an in-frame deletion in the gene encoding the motor component MotA is resistant to phage infection. We also generated two strains with point mutations in the motA gene resulting in replacement of the conserved charged residue Glu98, which is important for modulation of rotary speed. A change to the neutral Gln caused the flagellar motor to rotate at a constant high speed, allowing a 2.2-fold-enhanced infectivity. A change to the positively charged Lys caused a jiggly motility phenotype with very slow flagellar rotation, which significantly reduced the efficiency of infection. In conclusion, flagellar number and length, as well as speed of flagellar rotation, are important determinants for infection by phage 7-7-1.
authors.affiliation: Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia, USA.
authors.forename: Jiun Y
authors.initials: JY
authors.lastname: Yen
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Broadway
authors.forename: Birgit E
authors.initials: BE
authors.lastname: Scharf
mesh_terms: D060054:Agrobacterium / Q000502:physiology* / Q000821:virology*; D001426:Bacterial Proteins / Q000235:genetics; D001435:Bacteriophages / Q000254:growth & development* / Q000502:physiology; D005407:Flagella / Q000502:physiology* / Q000821:virology*; D005408:Flagellin / Q000235:genetics; D017353:Gene Deletion; D008124:Locomotion; D020125:Mutation, Missense; D012231:Rhizobium; D053585:Virus Attachment
chemical_list: D001426:Bacterial Proteins; C063010:MotA protein, Bacteria; D005408:Flagellin; C067138:flaA protein, bacteria; C416532:flaB flagellin; C076733:FlaD protein, Bacteria