title: Global gene expression and functional network analysis of gastric cancer identify extended pathway maps and GPRC5A as a potential biomarker.
abstract: To get more understanding of the molecular mechanisms underlying gastric cancer, 25 paired samples were applied to gene expression microarray analysis. Here, expression microarray, quantitative reverse transcription-PCR (qRT-PCR) and immunohistochemical analysis indicated that GPRC5A was significantly elevated in gastric cancer tissues. The integrative network analysis of deregulated genes generated eight subnetworks. We also mapped copy number variations (CNVs) and associated mRNA expression changes into pathways and identified WNT, RTK-Ras-PI3K-AKT, NF-κB, and PLAU-JAK-STAT pathways involved in proliferation, evading apoptosis and sustained angiogenesis, respectively. Taken together, our results reveal several interesting genes including GPRC5A as potential biomarkers for gastric cancer, and highlight more systematical insight of deregulated genes in genetic pathways of gastric carcinogenesis.
authors.affiliation: State Key Laboratory of Medical Genomics and Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cheng
authors.forename: Sheng
authors.initials: S
authors.lastname: Yang
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Yang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Huasheng
authors.initials: H
authors.lastname: Xiao
authors.forename: Hengjun
authors.initials: H
authors.lastname: Gao
authors.forename: Xiaxing
authors.initials: X
authors.lastname: Deng
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis*; D049109:Cell Proliferation; D056915:DNA Copy Number Variations; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D043562:Receptors, G-Protein-Coupled / Q000032:analysis* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C116760:GPRC5A protein, human; D043562:Receptors, G-Protein-Coupled