title: Anandamide externally added to lipid vesicles containing trapped fatty acid amide hydrolase (FAAH) is readily hydrolyzed in a sterol-modulated fashion.
abstract: We show that anandamide (AEA) externally added to model membrane vesicles containing trapped fatty acid amide hydrolyase (FAAH) can be readily hydrolyzed, demonstrating facile, rapid anandamide movement across the lipid bilayer. The rate of hydrolysis is significantly facilitated by cholesterol and coprostanol, but not by cholesterol sulfate. The effects of sterol upon hydrolysis by FAAH bound to the outer surface of the bilayer were much smaller, although they followed the same pattern. We propose the facilitation of hydrolysis is a combination of the effects of sterol on accessibility of membrane-inserted endocannabinoids to surface protein, and on the rate of endocannabinod transport across the membrane bilayer.
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York 11794, United States.
authors.forename: Martin
authors.initials: M
authors.lastname: Kaczocha
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Lin
authors.forename: Lindsay D
authors.initials: LD
authors.lastname: Nelson
authors.forename: Michelle K
authors.initials: MK
authors.lastname: McKinney
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Cravatt
authors.forename: Erwin
authors.initials: E
authors.lastname: London
authors.forename: Dale G
authors.initials: DG
authors.lastname: Deutsch
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry / Q000378:metabolism*; D001095:Arachidonic Acids / Q000737:chemistry / Q000378:metabolism*; D063388:Endocannabinoids / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008567:Membranes, Artificial; D053284:Polyunsaturated Alkamides / Q000737:chemistry / Q000378:metabolism*; D013261:Sterols / Q000737:chemistry / Q000378:metabolism*; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000581:Amidohydrolases; D001095:Arachidonic Acids; D063388:Endocannabinoids; D008081:Liposomes; D008567:Membranes, Artificial; D053284:Polyunsaturated Alkamides; D013261:Sterols; D000099252:Fatty Acid Amide Hydrolases; C078814:anandamide