title: Cell-free selection of domain antibodies by in vitro compartmentalization.
abstract: Efficient identification of antibodies, or any fragments thereof, displaying desired specificity and affinity is critical for the development of novel immunotherapeutics. Here we describe the adaptation of in vitro compartmentalization for the cell-free selection of Vκ and VH domain antibodies (dAbs™) from large combinatorial libraries. The dAbs™ are in vitro expressed in fusion to the N-terminus of single-chain variant of phage P22 Arc repressor DNA-binding domain that links the compartmentally expressed protein molecules to their encoding PCR fragment-based genes via cognate operator sites present on the DNA. Libraries of up to 10(10) in size can be rapidly assembled and selected for improved affinity in equilibrium and off-rate conditions.
authors.affiliation: Innovation Biopharm Discovery Unit, Biopharm R&D, GlaxoSmithKline Plc, Cambridge, UK. armin.sepp@gsk.com
authors.forename: Armin
authors.initials: A
authors.lastname: Sepp
authors.forename: Andrew
authors.initials: A
authors.lastname: Griffiths
mesh_terms: D061505:Cell Surface Display Techniques; D002474:Cell-Free System; D004655:Emulsions; D015723:Gene Library; D011485:Protein Binding / Q000276:immunology; D061905:Single-Domain Antibodies / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D004655:Emulsions; D061905:Single-Domain Antibodies