title: Targeted multiplex imaging mass spectrometry with single chain fragment variable (scfv) recombinant antibodies.
abstract: Recombinant scfv antibodies specific for CYP1A1 and CYP1B1 P450 enzymes were combined with targeted imaging mass spectrometry to simultaneously detect the P450 enzymes present in archived, paraffin-embedded, human breast cancer tissue sections. By using CYP1A1 and CYP1B1 specific scfv, each coupled to a unique reporter molecule (i.e., a mass tag) it was possible to simultaneously detect multiple antigens within a single tissue sample with high sensitivity and specificity using mass spectrometry. The capability of imaging multiple antigens at the same time is a significant advance that overcomes technical barriers encountered when using present day approaches to develop assays that can simultaneously detect more than a single antigen in the same tissue sample.
authors.affiliation: Mass Spectrometry Research Center, School of Medicine, Vanderbilt University, Nashville, TN, USA. gthiery@rics.bwh.harvard.edu
authors.forename: Gwendoline
authors.initials: G
authors.lastname: Thiery
authors.forename: Ray L
authors.initials: RL
authors.lastname: Mernaugh
authors.forename: Heping
authors.initials: H
authors.lastname: Yan
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Spraggins
authors.forename: Junhai
authors.initials: J
authors.lastname: Yang
authors.forename: Fritz F
authors.initials: FF
authors.lastname: Parl
authors.forename: Richard M
authors.initials: RM
authors.lastname: Caprioli
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D001189:Aryl Hydrocarbon Hydroxylases / Q000737:chemistry / Q000276:immunology; D001360:Avidin / Q000737:chemistry / Q000276:immunology; D001710:Biotin / Q000737:chemistry / Q000276:immunology; D001940:Breast / Q000737:chemistry; D001943:Breast Neoplasms / Q000737:chemistry; D019363:Cytochrome P-450 CYP1A1 / Q000737:chemistry / Q000276:immunology; D065633:Cytochrome P-450 CYP1B1; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012680:Sensitivity and Specificity; D057127:Single-Chain Antibodies / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; D001360:Avidin; D001710:Biotin; D001189:Aryl Hydrocarbon Hydroxylases; C569463:CYP1A1 protein, human; C585496:CYP1B1 protein, human; C585498:Cyp1b1 protein, mouse; C585499:Cyp1b1 protein, rat; D019363:Cytochrome P-450 CYP1A1; D065633:Cytochrome P-450 CYP1B1