title: Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of ade1 or ade2 mutants.
abstract: We identified Saccharomyces cerevisiae mutants with 100% higher intracellular glutathione using 1-methyl-3-nitro-1-nitrosoguanidine mutagenesis. This method employs visual selection of the most pigmented colonies among met30 strains carrying ade1 and ade2 mutations. Since the method does not involve genetic engineering, the mutants are suitable for use in the food industry.
authors.affiliation: Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhny proezd 1, 117545 Moscow, Russia.
authors.forename: Marina G
authors.initials: MG
authors.lastname: Tarutina
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Dutova
authors.forename: Inna E
authors.initials: IE
authors.lastname: Yezhova
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nishiuchi
authors.forename: Sergey P
authors.initials: SP
authors.lastname: Sineoky
mesh_terms: D002469:Cell Separation / Q000379:methods; D003116:Color; D019649:Food Industry / Q000379:methods; D005800:Genes, Fungal / Q000235:genetics; D005978:Glutathione / Q000032:analysis* / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D010858:Pigmentation / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D013455:Sulfur / Q000032:analysis*
chemical_list: D013455:Sulfur; D005978:Glutathione