title: Isoflavones suppress the expression of the FcεRI high-affinity immunoglobulin E receptor independent of the estrogen receptor.
abstract: Isoflavones found in soybeans and soy products possess clinically relevant properties. However, the anti-allergic effect of isoflavones has been poorly studied. We examined the effects of isoflavones, genistein, daidzein, and equol, on the expression of the high-affinity immunoglobulin E (IgE) receptor, FcεRI, which plays a central role in IgE-mediated allergic response. Flow cytometric analysis showed that all of these isoflavones reduced the cell surface expression of FcεRI on mouse bone-marrow-derived mast cells and human basophilic KU812 cells. All isoflavones decreased the levels of the FcεRIα mRNA in the cells. Genistein reduced the mRNA expression of the β chain, and daidzein and equol downregulated that of the γ chain. The suppressive effects of isoflavones on FcεRI expression were unaffected by ICI 182,780, an estrogen receptor antagonist, suggesting that these effects were independent of estrogen receptors.
authors.affiliation: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 812-8581, Japan.
authors.forename: Shuya
authors.initials: S
authors.lastname: Yamashita
authors.forename: Shuntaro
authors.initials: S
authors.lastname: Tsukamoto
authors.forename: Motofumi
authors.initials: M
authors.lastname: Kumazoe
authors.forename: Yoon-hee
authors.initials: YH
authors.lastname: Kim
authors.forename: Koji
authors.initials: K
authors.lastname: Yamada
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Tachibana
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology; D001491:Basophils / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line / Q000187:drug effects; D060754:Equol / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D000077267:Fulvestrant; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D011960:Receptors, Estrogen / Q000378:metabolism*; D017455:Receptors, IgE / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry
chemical_list: D018926:Anti-Allergic Agents; D004965:Estrogen Antagonists; C543355:FcepsilonRI alpha-chain, human; D007529:Isoflavones; D011960:Receptors, Estrogen; D017455:Receptors, IgE; D000077267:Fulvestrant; D007073:Immunoglobulin E; D004958:Estradiol; D060754:Equol; C004742:daidzein; D019833:Genistein; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3