title: First description of ColE-type plasmid in Aeromonas spp. carrying quinolone resistance (qnrS2) gene.
abstract: OBJECTIVE
  Isolation and full sequence analysis of ColE-type plasmid, which carries the qnrS2 gene.
  
  
  RESULTS
  Quinolone resistance (qnrS2) gene-carrying plasmids were isolated from Aeromonas sobria and Aeromonas hydrophila strains, and plasmid sequencing was achieved by a primer-walking approach. The total sizes of these plasmids (pAQ2-1 and pAQ2-2) were 6900 bp and 6903 bp, respectively, and they were 99·1% identical to each other. The genes (oriV and repA) for plasmid replication were organized similar to the corresponding genes in the ColE2-type plasmids, pAsa3 and pAsa1, isolated from Aeromonas salmonicida subsp. salmonicida, but the gene (mobA) for mobilization was homologue to ColE1-type plasmid (pAsa2) from Aer. salmonicida subsp. salmonicida. Additionally, the qnrS2 gene was part of a mobile insertion cassette element in the plasmid.
  
  
  CONCLUSIONS
  Two plasmids were assumed to be the same plasmid, and this identification of a plasmid-mediated qnrS2 gene from the two different strains underlines a possible diffusion of these resistance determinants in an aquaculture system.
  
  
  CONCLUSIONS
  This is the first finding of the ColE-type plasmid carrying the qnrS2 gene.
authors.affiliation: Laboratory of Aquatic Animal Medicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea.
authors.forename: J E
authors.initials: JE
authors.lastname: Han
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: C H
authors.initials: CH
authors.lastname: Choresca
authors.forename: S P
authors.initials: SP
authors.lastname: Shin
authors.forename: J W
authors.initials: JW
authors.lastname: Jun
authors.forename: J Y
authors.initials: JY
authors.lastname: Chai
authors.forename: S C
authors.initials: SC
authors.lastname: Park
mesh_terms: D000333:Aeromonas / Q000187:drug effects / Q000235:genetics*; D000818:Animals; D017756:Aquaculture; D060126:Characidae / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D009990:Oryzias / Q000382:microbiology; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification; D015363:Quinolones / Q000494:pharmacology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D015363:Quinolones