title: Proteomic analysis on salicylic acid-induced salt tolerance in common wheat seedlings (Triticum aestivum L.).
abstract: The influence of salicylic acid (SA) on the salt tolerance mechanism in seedlings of common wheat (Triticum aestivum L.) was investigated using physiological measurements combined with global expression profiling (proteomics). In the present study, 0.5mM SA significantly reduced NaCl-induced growth inhibition in wheat seedlings, manifesting as increased fresh weights, dry weights, and photosynthetic pigments, but decreased lipid peroxidation. Two-week-old wheat seedlings treated with 0.5mM SA, 250 mM NaCl and 250 mM NaCl+0.5mM SA for 3 days were used for the proteomic analyses. In total, 39 proteins differentially regulated by both salt and SA were revealed by 2D PAGE, and 38 proteins were identified by MALDI-TOF/TOF MS. The identified proteins were involved in various cellular responses and metabolic processes including signal transduction, stress defense, energy, metabolism, photosynthesis, and others of unknown function. All protein spots involved in signal transduction and the defense response were significantly upregulated by SA under salt stress, suggesting that these proteins could play a role in the SA-induced salt resistance in wheat seedlings.
authors.affiliation: The National Engineering Research Centre for Wheat, the Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou, Henan Province,450002, China. zkangg@163.com
authors.forename: Guozhang
authors.initials: G
authors.lastname: Kang
authors.forename: Gezi
authors.initials: G
authors.lastname: Li
authors.forename: Beibei
authors.initials: B
authors.lastname: Zheng
authors.forename: Qiaoxia
authors.initials: Q
authors.lastname: Han
authors.forename: Chenyang
authors.initials: C
authors.lastname: Wang
authors.forename: Yunji
authors.initials: Y
authors.lastname: Zhu
authors.forename: Tiancai
authors.initials: T
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D015227:Lipid Peroxidation; D010788:Photosynthesis; D010940:Plant Proteins / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D020156:Salicylic Acid / Q000494:pharmacology*; D055049:Salt Tolerance / Q000187:drug effects*; D036226:Seedlings / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry*
chemical_list: D010940:Plant Proteins; D020156:Salicylic Acid