title: Rhamnetin-induced suppression of clonal expansion during early stage of adipogenesis.
abstract: Adipocyte differentiation plays a pivotal role in the progression of obesity which is a major risk factor for several diseases such as diabetes, hypertension and coronary heart disease. In this study, the inhibitory effect of rhamnetin, a flavonoid compound, on adipogenesis in 3T3-L1 cells was investigated. Rhamnetin decreased the accumulation of lipid droplets, and inhibited the elevation of triglyceride content in the adipocytes (IC(50) = 17.3 μM). The expressions of PPARγ, C/EBPα, and perilipin, adipocyte differentiation markers, were significantly reduced by rhamnetin. Triglyceride biosynthesis and clonal expansion of adipocytes were completely inhibited during the early stage by rhamnetin. Additionally, rhamnetin significantly decreased the expression of C/EBPβ, an early stage marker. Our results indicate that suppression of clonal expansion during the early stage of adipogenesis by rhamnetin may be associated with inhibition of the C/EBPβ, C/EBPα, and PPARγ pathways.
authors.affiliation: College of Pharmacy and Medical Research Center, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: So-Young
authors.initials: SY
authors.lastname: Ji
authors.forename: Kyeong-Mi
authors.initials: KM
authors.lastname: Choi
authors.forename: Youn-Sun
authors.initials: YS
authors.lastname: Lee
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Yu
authors.forename: Dong-Mi
authors.initials: DM
authors.lastname: Shin
authors.forename: Seunghyun
authors.initials: S
authors.lastname: Lee
authors.forename: Kwang-Sik
authors.initials: KS
authors.lastname: Yoo
authors.forename: Yong-Moon
authors.initials: YM
authors.lastname: Lee
authors.forename: Yeo-Pyo
authors.initials: YP
authors.lastname: Yun
authors.forename: Hwan-Soo
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002999:Clone Cells; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D047495:PPAR gamma / Q000378:metabolism; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism
chemical_list: D015415:Biomarkers; D022763:CCAAT-Enhancer-Binding Protein-alpha; D002352:Carrier Proteins; D047495:PPAR gamma; D000070778:Perilipin-1; D010750:Phosphoproteins; D014280:Triglycerides; C063423:rhamnetin; D011794:Quercetin