title: Structure of human Rack1 protein at a resolution of 2.45 Å.
abstract: The crystal structure of human receptor for activated C-kinase 1 (hRack1) protein is reported at 2.45 Å resolution. The crystals belongs to space group P4(1)2(1)2, with three molecules per asymmetric unit. The hRack1 structure features a sevenfold β-propeller, with each blade housing a sequence motif that contains a strictly conserved Trp, the indole group of which is embedded between adjacent blades. In blades 1-5 the imidazole group of a His residue is wedged between the side chains of a Ser residue and an Asp residue through two hydrogen bonds. The hRack1 crystal structure forms a starting basis for understanding the remarkable scaffolding properties of this protein.
authors.affiliation: School of Biological Sciences, Nanyang Technological University, Singapore.
authors.forename: David
authors.initials: D
authors.lastname: Ruiz Carrillo
authors.forename: Ramya
authors.initials: R
authors.lastname: Chandrasekaran
authors.forename: Martina
authors.initials: M
authors.lastname: Nilsson
authors.forename: Tobias
authors.initials: T
authors.lastname: Cornvik
authors.forename: Chong Wai
authors.initials: CW
authors.lastname: Liew
authors.forename: Suet Mien
authors.initials: SM
authors.lastname: Tan
authors.forename: Julien
authors.initials: J
authors.lastname: Lescar
mesh_terms: D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D019204:GTP-Binding Proteins / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000737:chemistry*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D040681:Structural Homology, Protein
chemical_list: D009363:Neoplasm Proteins; C419368:RACK1 protein, human; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D019204:GTP-Binding Proteins