title: Triterpene saponins from Pleurospermum kamtschaticum and their biological activity.
abstract: Eleven new triterpene saponins (1-11), together with fourteen known triterpene and triterpene saponins (12-25) were isolated from a MeOH extract of Pleurospermum kamtschaticum HOFFMANN (Umbelliferae). The chemical structures of the new compounds (1-11) were determined by means of MS, (1)H-NMR, (13)C-NMR, correlated spectroscopy (COSY), heteronuclear multiple bond correlation (HMBC), total correlated spectroscopy (TOCSY) and nuclear Overhauser effect spectroscopy (NOESY) to be pleurosaponin A (1)-K (11). The isolated compounds were tested for their cytotoxicity against four human tumor cell lines (A549, SK-OV-3, SK-MEL-2, HCT15) in vitro using the sulforhodamine B bioassay (SRB) assay. All compounds showed little cytotoxicity against tested cell lines (IC(50) >30 µM).
authors.affiliation: Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Il Kyun
authors.initials: IK
authors.lastname: Lee
authors.forename: Sang Un
authors.initials: SU
authors.lastname: Choi
authors.forename: Kang Ro
authors.initials: KR
authors.lastname: Lee
mesh_terms: D019661:Apiaceae / Q000737:chemistry*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013057:Spectrum Analysis / Q000379:methods; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D012503:Saponins; D014315:Triterpenes