title: A defensin-like peptide from Phaseolus vulgaris cv. 'King Pole Bean'.
abstract: A 5447 Da antifungal peptide with an N-terminal sequence highly homologous to plant defensins was purified from Phaseolus vulgaris cv. 'King Pole Bean' by anion-exchange chromatography on Q Sepharose and FPLC-gel filtration on Superdex 75. The isolated peptide inhibited growth of a number of fungal species, including Mycosphaerella arachidicola, Saccharomyces cerevisiae and Candida albicans, with IC(50) values of 3.9, 4.0 and 8.4 μM, respectively. Using the membrane non-permeable DNA-binding dye SYTOX green, it was found that the peptide increased the cell membrane permeability of M. arachidicola, S. cerevisiae and C. albicans.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong. jack1993@yahoo.com
authors.forename: Jack H
authors.initials: JH
authors.lastname: Wong
authors.forename: David C W
authors.initials: DC
authors.lastname: Ip
authors.forename: T B
authors.initials: TB
authors.lastname: Ng
authors.forename: Y S
authors.initials: YS
authors.lastname: Chan
authors.forename: F
authors.initials: F
authors.lastname: Fang
authors.forename: W L
authors.initials: WL
authors.lastname: Pan
mesh_terms: D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D023082:Defensins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects; D008969:Molecular Sequence Data; D027805:Phaseolus / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D023082:Defensins