title: Evaluation of CytoDiff™ on cord blood WBC differential.
abstract: BACKGROUND
  An umbilical cord blood bank was recently opened in our institution as an alternative source of hematopoietic stem cells. Before inclusion of a cord blood in an international register, a WBC with differential is requested, among others. Currently, the reference method is the microscopic manual count, and we sought to evaluate the routine flow cytometric method (CytoDiff™) as an alternative.
  
  
  METHODS
  A total of 161 cord bloods were analyzed between November 2010 and February 2011. WBC differentials were determined for each sample, by (i) the cell counter (DxH800), (ii) a manual review, and (iii) the flow cytometry using the CytoDiff™ antibody cocktail.
  
  
  RESULTS
  Correlation coefficients between flow cytometry and microscopic count were satisfying for neutrophils, lymphocytes, and immature granulocytes and acceptable for eosinophils. On the other hand, we found lower correlation coefficient for basophils and monocytes. Monocytes' correlation was better when comparing flow cytometry with cell counter.
  
  
  CONCLUSIONS
  The flow cytometric approach is suitable to realize cord blood WBC differential and allows for the identification of additional cell subsets.
authors.affiliation: CHU Rennes, Laboratoire d'Hématologie, Pôle de Biologie, Rennes, France.
authors.forename: F
authors.initials: F
authors.lastname: Gac
authors.forename: J B
authors.initials: JB
authors.lastname: Thibert
authors.forename: C
authors.initials: C
authors.lastname: Le Berre
authors.forename: J
authors.initials: J
authors.lastname: Le Priol
authors.forename: G
authors.initials: G
authors.lastname: Semana
authors.forename: T
authors.initials: T
authors.lastname: Fest
authors.forename: M
authors.initials: M
authors.lastname: Roussel
mesh_terms: D001771:Blood Banks; D005260:Female; D005312:Fetal Blood / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007202:Indicators and Reagents / Q000378:metabolism*; D007958:Leukocyte Count / Q000379:methods*; D011247:Pregnancy; D015203:Reproducibility of Results; D047929:Term Birth
chemical_list: D007202:Indicators and Reagents