title: Preparation of core-shell poly(L-lactic) acid-nanocrystalline apatite hollow microspheres for bone repairing applications.
abstract: In this paper, hybrid inorganic-organic core-shell hollow microspheres, made of poly(L-lactic acid) (PLLA) and biomimetic nano apatites (HA), were prepared from biodegradable and biocompatible substances, suitable for bone tissue applications. Preparation is started from Pickering emulsification, i.e., solid particle-stabilized emulsions in the absence of any molecular surfactant, where solid particles adsorbed to an oil-water interface. Stable oil-in-water emulsions were produced using biomimetic 20 nm sized HA nanocrystals as particulate emulsifier and a dichloromethane (CH(2)Cl(2)) solution of PLLA as oil phase. Hybrid hollow PLLA microspheres at three different HA nanocrystals surface coverage, ranging from 10 to 50 μm, were produced. The resulting materials were completely characterized with spectroscopic, calorimetric and microscopic techniques and the cytocompatibility was established by indirect contact tests with both fibroblasts and osteoblasts and direct contact with these latter. They displayed a high level of cytocompatibility and thus represent promising materials for drug delivery systems, cell carriers and scaffolds for regeneration of bone useful in the treatment of orthopaedic, maxillofacial and dental fields.
authors.affiliation: Dipartimento di Scienze della Salute, Università del Piemonte Orientale, Novara, Italy. michele.iafisco@unibo.it
authors.forename: Michele
authors.initials: M
authors.lastname: Iafisco
authors.forename: Barbara
authors.initials: B
authors.lastname: Palazzo
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ito
authors.forename: Makoto
authors.initials: M
authors.lastname: Otsuka
authors.forename: Mamoru
authors.initials: M
authors.lastname: Senna
authors.forename: Josè Manuel
authors.initials: JM
authors.lastname: Delgado-Lopez
authors.forename: Jaime
authors.initials: J
authors.lastname: Gomez-Morales
authors.forename: Anna
authors.initials: A
authors.lastname: Tampieri
authors.forename: Maria
authors.initials: M
authors.lastname: Prat
authors.forename: Lia
authors.initials: L
authors.lastname: Rimondini
mesh_terms: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002152:Calorimetry, Differential Scanning; D002478:Cells, Cultured; D003460:Crystallization; D005455:Fluorescent Antibody Technique; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010006:Osteoblasts / Q000166:cytology; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)