title: Vitamin D receptor is not essential for extracellular signal-related kinase phosphorylation by vitamin D(3) in human Caco-2/TC7 cells.
abstract: Vitamin D(3) initiated rapid extracellular signal-related kinase (ERK) phosphorylation, but the contribution of vitamin D receptor (VDR) to this event is unclear. We investigated the use of RNA interference (RNAi) to knockdown VDR. RNAi downregulated VDR as well as its targeted gene expression, but vitamin D(3) dependent ERK phosphorylation remained. Thus VDR might not be involved in ERK phosphorylation by vitamin D(3).
authors.affiliation: Section of Food and Nutrition Labeling, Department of Food Function and Labeling, National Institute of Health and Nutrition, 1-23-1 Toyama, Tokyo 162-8636, Japan. junyama@nih.go.jp
authors.forename: Jun
authors.initials: J
authors.lastname: Yamauchi
authors.forename: Mariko
authors.initials: M
authors.lastname: Sekiguchi
authors.forename: Tomomi
authors.initials: T
authors.lastname: Shirai
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Ishimi
mesh_terms: D018938:Caco-2 Cells; D002762:Cholecalciferol / Q000378:metabolism / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D034741:RNA, Small Interfering / Q000235:genetics; D018167:Receptors, Calcitriol / Q000037:antagonists & inhibitors / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D018167:Receptors, Calcitriol; D002762:Cholecalciferol; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3