title: A method for simultaneous quantification of phospholipid species by routine 31P NMR.
abstract: We report a (31)P NMR assay for quantification of aqueous phospholipid samples. Using a capillary with trimethylphosphate as internal standard, the limit of quantification is 1.30 mM. Comparison of the (31)P NMR quantification method in aqueous buffer and in organic solvent revealed that the two methods are equal within experimental error. Changing the pH of the buffer enables peak separation for different phospholipid species. This is an advantage compared to the commercial enzyme assay based on phospholipase D and choline oxidase. The reported method, using routine (31)P NMR equipment, is suitable when fast results of a limited number of samples are requested.
authors.affiliation: Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense, Denmark. trettenes@sdu.dk
authors.forename: Ulla
authors.initials: U
authors.lastname: Brinkmann-Trettenes
authors.forename: Paul C
authors.initials: PC
authors.lastname: Stein
authors.forename: Beate
authors.initials: B
authors.lastname: Klösgen
authors.forename: Annette
authors.initials: A
authors.lastname: Bauer-Brandl
mesh_terms: D002021:Buffers; D002138:Calibration; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy / Q000592:standards; D010743:Phospholipids / Q000378:metabolism*; D010759:Phosphorus Isotopes; D012015:Reference Standards; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry
chemical_list: D002021:Buffers; D010743:Phospholipids; D010759:Phosphorus Isotopes; D012997:Solvents