title: Insect ryanodine receptor: distinct but coupled insecticide binding sites for [N-C(3)H(3)]chlorantraniliprole, flubendiamide, and [(3)H]ryanodine.
abstract: Radiolabeled anthranilic diamide insecticide [N-C(3)H(3)]chlorantraniliprole was synthesized at high specific activity. It was compared with phthalic diamide insecticide flubendiamide and [(3)H]ryanodine in radioligand binding studies with house fly muscle membranes to provide the first direct evidence with a native insect ryanodine receptor that the major anthranilic and phthalic diamide insecticides bind at different allosterically coupled sites, i.e., there are three distinct Ca(2+)-release channel targets for insecticide action.
authors.affiliation: Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA.
authors.forename: André K
authors.initials: AK
authors.lastname: Isaacs
authors.forename: Suzhen
authors.initials: S
authors.lastname: Qi
authors.forename: Richmond
authors.initials: R
authors.lastname: Sarpong
authors.forename: John E
authors.initials: JE
authors.lastname: Casida
mesh_terms: D000818:Animals; D001549:Benzamides / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002244:Carbon / Q000737:chemistry; D019476:Insect Proteins / Q000737:chemistry / Q000378:metabolism*; D007313:Insecta; D007306:Insecticides / Q000737:chemistry / Q000378:metabolism*; D009584:Nitrogen / Q000737:chemistry; D011485:Protein Binding; D012433:Ryanodine / Q000737:chemistry / Q000378:metabolism*; D019837:Ryanodine Receptor Calcium Release Channel / Q000737:chemistry / Q000378:metabolism*; D013450:Sulfones / Q000737:chemistry / Q000378:metabolism*; D014316:Tritium / Q000737:chemistry; D062367:ortho-Aminobenzoates / Q000737:chemistry / Q000378:metabolism*; D005466:Fluorocarbons; D010797:Phthalimides
chemical_list: D001549:Benzamides; D002118:Calcium; D002244:Carbon; D019476:Insect Proteins; D007306:Insecticides; D009584:Nitrogen; D012433:Ryanodine; D019837:Ryanodine Receptor Calcium Release Channel; D013450:Sulfones; D014316:Tritium; D062367:ortho-Aminobenzoates; C517733:chlorantranilipole; C508762:flubendiamide; D005466:Fluorocarbons; D010797:Phthalimides