title: [Expression of δ subunit of γ-aminobutyric acid A in hippocampus induced by insular-kindled rats].
abstract: OBJECTIVE
  To explore the expression of δ subunit-containing γ-aminobutyric acid(A) (GABA)(A) receptors in insular electrical kindled rats and analyze the significance of the findings.
  
  
  METHODS
  A total of 48 male Sprague-Dawley rats were divided randomly into 3 groups: kindled, sham-operated and control. Kindled group: chronic insular electrical kindled models of rats established by electrical stimulation. Sham-operated group: the same method of the kindled group without electrical stimulation.
  
  
  METHODS
  without surgery. The number of hippocampal neurons of rat epilepsy, sham-operated and control groups were detected by Nissl staining. The hippocampal expressions of δ subunit of these groups were tested by immunohistochemistry. The mRNA of δ subunit of hippocampus of these groups was detected by quantitative-polymerase chain reaction (q-PCR).
  
  
  RESULTS
  The findings of Nissl staining showed that there was no significant difference of the number of hippocampal neurons between epileptic and normal rats (P > 0.05). The findings of q-PCR showed that mRNA of δ subunit of the hippocampus of sham-operated and control groups represented much more than that of kindled group at Day 7 post-kindling (P < 0.01). The findings of immunohistochemistry showed that the hippocampal expression of δ subunit of sham-operated and control groups represented much more than that of kindled group at Day 7 post-kindling (P < 0.01).
  
  
  CONCLUSIONS
  The hippocampal expression of δ subunit-containing GABA(A) receptors decreases in insular epilepsy. It may play an important role in the occurrence and development of insular epilepsy.
authors.affiliation: Ningxia Medical University, Incubation Base of National Key Laboratory for Cerebrocranial Diseases, Yinchuan 750004, China.
authors.forename: En-jie
authors.initials: EJ
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Zi-mu
authors.initials: ZM
authors.lastname: Song
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Lu
authors.initials: L
authors.lastname: Miao
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004827:Epilepsy / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D007696:Kindling, Neurologic / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: D011963:Receptors, GABA-A