title: Expression of TNF-alpha mRNA, but not of TNF-alpha receptors mRNA, is detected in single murine oocyte and decreases during oocyte meiotic maturation: single-cell RT-PCR data.
abstract: BACKGROUND
  The present study was conducted to investigate the expression of TNF-alpha and its receptors (types I and II) in both oocytes with germinal vesicle and the first polar body in mice.
  
  
  METHODS
  Oocytes with intact germinal vesicle were isolated from mouse ovaries and subjected to in vitro maturation to obtain oocytes forming the first polar body. A reverse transcription polymerase chain reaction (RT-PCR) was used to examine the expression of TNF-alpha and its receptors at mRNA level.
  
  
  RESULTS
  mRNA TNF-alpha was expressed in single oocytes and its level was decreasing during transition from germinal vesicle to the first polar body stage. At the same time the expression of TNF-receptors was not observed in single oocyte.
  
  
  CONCLUSIONS
  These data are the important link in understanding of the molecular mechanisms regulating oocyte maturation as well as follicle development.
authors.affiliation: O.O.Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv, Ukraine. elena-shepel@ukr.net
authors.forename: O A
authors.initials: OA
authors.lastname: Shepel
authors.forename: V E
authors.initials: VE
authors.lastname: Dosenko
authors.forename: T Yu
authors.initials: TY
authors.lastname: Voznesenska
authors.forename: R I
authors.initials: RI
authors.lastname: Yanchiy
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D008540:Meiosis / Q000235:genetics; D051379:Mice; D008808:Mice, Inbred CBA; D009866:Oogenesis / Q000235:genetics*; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism*; D059705:Polar Bodies / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059010:Single-Cell Analysis; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha