title: [Antioxidant and antiaggregant effects of covalent bienzyme superoxide dismutase-chondroitin sulfate-catalase conjugate in platelet interactions].
abstract: A covalent bienzyme superoxide dismutase-chondroitin sulfate-catalase conjugate (SOD-CHS-CAT) demonstrated the dose-dependent inhibitory action on induced aggregation of platelets in the presence of hydrogen peroxide. Antioxidant activity of SOD-CHS-CAT appeared at much lower doses than for other CAT derivatives. We detected the antiaggregation effect of SOD-CHS-CAT for platelet aggregation induced by ADP, serotonin, TRAP (with their different mechanism of action). Novel properties of SOD-CHS-CAT confirmed with its action agains spread-eagle platelets on glass surface. The new characteristics of SOD-CHS-CAT conjugate underline the prospects of its biopharmaceutical development for antioxidant therapy.
authors.forename: A V
authors.initials: AV
authors.lastname: Vavaev
authors.forename: L I
authors.initials: LI
authors.lastname: Buriachkivskaia
authors.forename: E G
authors.initials: EG
authors.lastname: Tishchenko
authors.forename: I A
authors.initials: IA
authors.lastname: Uchitel'
authors.forename: A V
authors.initials: AV
authors.lastname: Maksimenko
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology*; D002417:Cattle; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D013482:Superoxide Dismutase / Q000494:pharmacology*
chemical_list: D000925:Anticoagulants; D000975:Antioxidants; D002809:Chondroitin Sulfates; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase