title: [Study on the extraction technology and hypoglycemic activity of lectin from Trichosanthes kirilowi].
abstract: OBJECTIVE
  To extract lectins from Trichosanthes kirilowi and study their hypoglycemic activity.
  
  
  METHODS
  The optimal extraction process included the following parameters were conformed by optimization analysis,lectins extracted from Trichosanthes kirilowi was achieved by ammonium sulfate precipitation; The agglutinate activity was determined by using the agglutination test with 5% human blood cells. Human hepatocarcinoma cell HepG2 and the alloxan-induced diabetic mice model were used to assess hypoglycemic activity of Lectin in Trichosanthes kirilowi.
  
  
  RESULTS
  The agglutination indexes of lectins extraction buffer were 32; The cell and mice tests indicated that the lectins exhibited hypoglycemic activity in the 70% saturation.
  
  
  CONCLUSIONS
  The optimum extraction technology is as follows: extraction with PBS, the material-water ratio is 1:30, the extraction time is 24 h, while the concentration of sodium chloride is 0 mol/L and pH is 7.2. Precipitate lectins by ammonium sulfate in the 70% saturation, centrifugal speed is 10 000 tracted from Trichosanthes kirilowi exposes proper hypoglycemic activity.
authors.affiliation: College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. qiongli1123@126.com
authors.forename: Qiong
authors.initials: Q
authors.lastname: Li
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Ye
authors.forename: Hong
authors.initials: H
authors.lastname: Zeng
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Xue-Gang
authors.initials: XG
authors.lastname: Li
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology*; D006384:Hemagglutination / Q000187:drug effects; D006386:Hemagglutination Tests; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors; D029778:Trichosanthes / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D037121:Plant Lectins; D000645:Ammonium Sulfate