title: Single-step reconstitution of apo-hemoproteins at the disruption stage of Escherichia coli cells.
abstract: Hemoproteins on their metal: We report a novel strategy for the reconstitution of hemoproteins with non-natural metal complexes; simple addition of manganese and ruthenium porphyrin to E. coli cells immediately prior to homogenization yields the reconstituted proteins. We believe that this simple approach could become a standard reconstitution method for hemoproteins.
authors.affiliation: Research Center for Materials Science, Nagoya University, Furo-cho, Nagoya, 464-8602, Japan.
authors.forename: Norifumi
authors.initials: N
authors.lastname: Kawakami
authors.forename: Osami
authors.initials: O
authors.lastname: Shoji
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry; D003460:Crystallization; D004926:Escherichia coli / Q000378:metabolism*; D006420:Hemeproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008345:Manganese / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D006420:Hemeproteins; D011166:Porphyrins; D011994:Recombinant Proteins; D008345:Manganese; D012428:Ruthenium