title: The innate immune protein Nod2 binds directly to MDP, a bacterial cell wall fragment.
abstract: Mammalian Nod2 is an intracellular protein that is implicated in the innate immune response to the bacterial cell wall and is associated with the development of Crohn's disease, Blau syndrome, and gastrointestinal cancers. Nod2 is required for an immune response to muramyl dipeptide (MDP), an immunostimulatory fragment of bacterial cell wall, but it is not known whether MDP binds directly to Nod2. We report the expression and purification of human Nod2 from insect cells. Using novel MDP self-assembled monolayers (SAMs), we provide the first biochemical evidence for a direct, high-affinity interaction between Nod2 and MDP.
authors.affiliation: Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA. cgrimes@udel.edu
authors.forename: Catherine Leimkuhler
authors.initials: CL
authors.lastname: Grimes
authors.forename: Lushanti De Zoysa
authors.initials: Lde Z
authors.lastname: Ariyananda
authors.forename: James E
authors.initials: JE
authors.lastname: Melnyk
authors.forename: Erin K
authors.initials: EK
authors.lastname: O'Shea
mesh_terms: D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000276:immunology*; D000818:Animals; D001419:Bacteria / Q000276:immunology*; D002460:Cell Line; D002473:Cell Wall / Q000276:immunology*; D003001:Cloning, Molecular; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D007313:Insecta / Q000166:cytology; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D053473:Nod2 Signaling Adaptor Protein; D011994:Recombinant Proteins; D000119:Acetylmuramyl-Alanyl-Isoglutamine