title: Homodimeric SV40 NLS peptide formed by disulfide bond as enhancer for gene delivery.
abstract: Recently, cysteine residue incorporation increased liposome-mediated transfection compared to unmodified peptide. Therefore, we designed novel modified SV40 NLS peptides, homodimeric (NLS-CTHD, NLS-NTHD) and closed structure (cyclic NLS), simply using disulfide bond between cysteines to develop more efficient and safe non-viral gene delivery system. The simple mix of NLS-CTHD among these novel transfection enhancing peptides with DNA increased the gene transfer potency of cationic liposomes more efficiently with no additional cytotoxicity.
authors.affiliation: Department of Physiology, College of Medicine, Yeungnam University, Daegu 705-717, Republic of Korea.
authors.forename: Bieong-Kil
authors.initials: BK
authors.lastname: Kim
authors.forename: Hyungu
authors.initials: H
authors.lastname: Kang
authors.forename: Kyung-Oh
authors.initials: KO
authors.lastname: Doh
authors.forename: Seong-Hye
authors.initials: SH
authors.lastname: Lee
authors.forename: Jong-Won
authors.initials: JW
authors.lastname: Park
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D003545:Cysteine / Q000737:chemistry; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004220:Disulfides / Q000737:chemistry*; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D014162:Transfection
chemical_list: D004220:Disulfides; D008081:Liposomes; D009842:Oligopeptides; C402304:SV40 nuclear localization peptide; D004247:DNA; D003545:Cysteine