title: Downregulation of nuclear respiratory factor-1 contributes to mitochondrial events induced by benzo(a)pyrene.
abstract: Environmental carcinogen benzo(a)pyrene (BaP) has been shown to be a genotoxicant that affects both nuclear DNA (nDNA) and mitochondrial DNA (mtDNA). Nuclear respiratory factor 1 (NRF-1) is a transcriptional activator of nuclear genes that encode a range of mitochondrial proteins including mitochondrial transcription factor A (mtTFA). However, the role of NRF-1 in BaP-induced mitochondrial event is not clear. We investigated the change of NRF-1 and mtTFA in human bronchial epithelial cells (16HBE) elicited by BaP. The results indicated that BaP induced cell apoptosis, total mitochondrial enzymes activities and ATP levels decrease in dose- and time-dependent manners, respectively. The transcription and protein levels of NRF-1 and mtTFA decreased at 48 h after 16 μM BaP treatment. Our results indicated downregulation of NRF-1 and mtTFA is involved in BaP-induced mitochondrial events.
authors.affiliation: Division of Preventive Medicine, Tongji University Medical School, Shanghai 200092, People's Republic of China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Bao
authors.forename: Yimin
authors.initials: Y
authors.lastname: Liu
authors.forename: Jue
authors.initials: J
authors.lastname: Li
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017209:Apoptosis; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001980:Bronchi / Q000166:cytology; D002274:Carcinogens, Environmental / Q000633:toxicity*; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D051266:Nuclear Respiratory Factor 1 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D002274:Carcinogens, Environmental; D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; D051266:Nuclear Respiratory Factor 1; D012333:RNA, Messenger; D014157:Transcription Factors; C422562:mitochondrial transcription factor A; D001564:Benzo(a)pyrene; D000255:Adenosine Triphosphate