title: Application of single-domain antibodies in tumor histochemistry.
abstract: High avidity, pentameric, single-domain antibodies, oligomerized through the B subunit of verotoxin, are excellent immunohistochemical reagents. The resulting molecules are termed pentabodies. Here, we describe the immunostaining of tissue sections with ES1, a pentabody recognizing CEACAM6 which is overexpressed in several cancers. The advantages of pentabodies, compared to conventional antibody reagents, in immunohistochemical studies are highlighted.
authors.affiliation: Division of Anatomical Pathology, Department of Pathology and Laboratory Medicine, The Ottawa Hospital, Ottawa, ON, Canada. ktmai@ottawahospital.on.ca
authors.forename: Kien T
authors.initials: KT
authors.lastname: Maik
authors.forename: C Roger
authors.initials: CR
authors.lastname: Mackenzie
mesh_terms: D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D061905:Single-Domain Antibodies
chemical_list: D061905:Single-Domain Antibodies