title: Microfluidic PCR combined with pyrosequencing for identification of allelic variants with phenotypic associations among targeted Salmonella genes.
abstract: A novel targeted massive parallel sequencing approach identified genetic variation in eight known or predicted fimbrial adhesins for 46 Salmonella strains. The results highlight associations between specific adhesin alleles, host species, and antimicrobial resistance. The differentiation of allelic variants has potential applications for diagnostic microbiology and epidemiological investigations.
authors.affiliation: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Yue
authors.forename: Robert
authors.initials: R
authors.lastname: Schmieder
authors.forename: Robert A
authors.initials: RA
authors.lastname: Edwards
authors.forename: Shelley C
authors.initials: SC
authors.lastname: Rankin
authors.forename: Dieter M
authors.initials: DM
authors.lastname: Schifferli
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000483:Alleles; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D014644:Genetic Variation; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D058507:Host Specificity; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D017720:Molecular Epidemiology / Q000379:methods; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*; D012475:Salmonella / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000502:physiology
chemical_list: D018829:Adhesins, Bacterial; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial