title: Anti-obesity effects of Boussingaulti gracilis Miers var. pseudobaselloides Bailey via activation of AMP-activated protein kinase in 3T3-L1 cells.
abstract: In a previous study, we demonstrated the anti-obesity and hypolipidemic effects of Boussingaulti gracilis Miers var. pseudobaselloides Bailey in high-fat diet-induced obese rats. The present study investigated the molecular mechanisms by which B. gracilis Miers var. pseudobaselloides Bailey ethanol extract (BGE) conferred antidifferentiation and anti-adipogenic effects in the 3T3-L1 preadipocyte differentiation model. BGE treatment significantly and dose-dependently suppressed lipid accumulation and down-regulated the expression of major transcription factors involved in adipogenesis, such as peroxisome proliferator-activated receptor-γ, CCAAT/enhancer binding protein α, sterol regulatory element-binding proteins, and their target genes. It is important that treatment with BGE increased phosphorylation of AMP-activated protein kinase (AMPK), which is one of the rate-limiting enzymes in the fatty acid synthesis pathway, and its direct downstream protein, acetyl-coenzyme A carboxylase. These results suggest that BGE may exert anti-adipogenic effects through regulation of AMPK activity and expression of genes involved in lipogenesis.
authors.affiliation: Department of Hygienic Chemistry, College of Pharmacy, Kyung Hee University, Seoul, Korea.
authors.forename: Hana
authors.initials: H
authors.lastname: Kim
authors.forename: Se-Young
authors.initials: SY
authors.lastname: Choung
mesh_terms: D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D017667:Adipocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D050156:Adipogenesis; D000818:Animals; D019440:Anti-Obesity Agents / Q000378:metabolism*; D019587:Dietary Supplements; D019669:Embryophyta / Q000737:chemistry*; D020536:Enzyme Activators / Q000378:metabolism*; D005786:Gene Expression Regulation; D000960:Hypolipidemic Agents / Q000378:metabolism; D050356:Lipid Metabolism; D051379:Mice; D010766:Phosphorylation; D010936:Plant Extracts / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry*; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D019440:Anti-Obesity Agents; D020536:Enzyme Activators; D000960:Hypolipidemic Agents; D010936:Plant Extracts; D012333:RNA, Messenger; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase