title: Duodenal enteroendocrine I-cells contain mRNA transcripts encoding key endocannabinoid and fatty acid receptors.
abstract: Enteroendocrine cells have a critical role in regulation of appetite and energy balance. I-cells are a subtype of enteroendocrine cells localized in duodenum that release cholecystokinin in response to ingested fat and amino-acids. Despite their potentially pivotal role in nutrient sensing and feeding behaviour, native I-cells have previously been difficult to isolate and study. Here we describe a robust protocol for the isolation and characterization of native duodenal I-cells and additionally, using semi-quantitative RT-PCR we determined that mouse duodenal I-cells contain mRNA transcripts encoding key fatty acid and endocannabinoid receptors including the long chain fatty acid receptors GPR40/FFAR1, GPR120/O3FAR1; short chain fatty acid receptors GPR41/FFAR3 and GPR43/FFAR2; the oleoylethanolamide receptor GPR119 and the classic endocannabinoid receptor CB1. These data suggest that I-cells sense a wide range of gut lumen nutrients and also have the capacity to respond to signals of fatty-acid derivatives or endocannabinoid peptides.
authors.affiliation: Faculty of Life Sciences, The University of Manchester, Manchester, United Kingdom.
authors.forename: Alexandros G
authors.initials: AG
authors.lastname: Sykaras
authors.forename: Claire
authors.initials: C
authors.lastname: Demenis
authors.forename: R Maynard
authors.initials: RM
authors.lastname: Case
authors.forename: John T
authors.initials: JT
authors.lastname: McLaughlin
authors.forename: Craig P
authors.initials: CP
authors.lastname: Smith
mesh_terms: D000818:Animals; D018076:DNA, Complementary; D004386:Duodenum / Q000166:cytology / Q000378:metabolism*; D063388:Endocannabinoids / Q000378:metabolism*; D019858:Enteroendocrine Cells / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D015870:Gene Expression; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D012333:RNA, Messenger / Q000235:genetics*; D011949:Receptors, Cholecystokinin / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics* / Q000378:metabolism; D015203:Reproducibility of Results; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D063388:Endocannabinoids; D005227:Fatty Acids; D012333:RNA, Messenger; D011949:Receptors, Cholecystokinin; D043562:Receptors, G-Protein-Coupled