title: Subzero 24-hr nonfreezing rat heart preservation: a novel preservation method in a variable magnetic field.
abstract: BACKGROUND
  A new supercooling system using a variable magnetic field has been recently developed. Subzero nonfreezing preservation has been believed to be a beneficial method because of the lower metabolic rate. The purpose of this study was to evaluate the hemodynamic and metabolic effects of rat heart preservation in a variable magnetic field without cryoprotectants.
  
  
  METHODS
  Rat hearts were perfused ex vivo for 120 min after 24-hr preservation in two groups (n=6 each): (1) conventional storage group, in which the hearts were stored at 4°C, and (2) the subzero group, in which the hearts were preserved at -3°C in a variable magnetic field.
  
  
  RESULTS
  Reperfusion cardiac performance after preservation was significantly preserved in the subzero group compared with the conventional group with respect to heart rate, coronary flow, the peak positive dP/dt, and the peak negative dP/dt (P<0.05). Edema after reperfusion was significantly decreased (P<0.05), and the adenosine triphosphate level was higher in the subzero group (P<0.05).
  
  
  CONCLUSIONS
  The rat hearts preserved in a variable magnetic field at -3°C showed better hemodynamic and metabolic performance than those preserved using conventional storage at 4°C.
authors.affiliation: Department of General and Cardiothoracic Surgery, Kanazawa University, Kanazawa, Ishikawa, Japan. hirokikato@e-mail.jp
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kato
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Tomita
authors.forename: Shoujiro
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohtake
authors.forename: Go
authors.initials: G
authors.lastname: Watanabe
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003080:Cold Temperature / Q000009:adverse effects; D003326:Coronary Circulation; D015925:Cryopreservation; D006339:Heart Rate; D016027:Heart Transplantation; D060526:Magnetic Fields / Q000009:adverse effects; D009200:Myocardial Contraction; D015428:Myocardial Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism; D009926:Organ Preservation / Q000009:adverse effects / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D016277:Ventricular Function, Left; D017725:Ventricular Pressure; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D000255:Adenosine Triphosphate