title: Effect of red wine consumption on biomarkers of oxidative stress.
abstract: OBJECTIVE
  To evaluate the effect of acute and chronic consumption of red wine or de-alcoholized red wine with a similar antioxidant capacity on plasma total antioxidant capacity (TEAC), nuclear factor-κB (NF-κB) activity and F2-isoprostanes (8-iso-PGF(2α)) in healthy men.
  
  
  METHODS
  Nineteen healthy men with an increased waist circumference (≥94 cm) and a body mass index above 25 kg/m(2) participated in a randomized, controlled crossover design trial. They daily consumed 450 ml of red wine (four drinks; 41.4 g alcohol) or 450 ml of de-alcoholized red wine during dinner for 4 weeks each. On the last day of each treatment period, blood was collected before and 1 h after a standardized dinner with red wine or de-alcoholized red wine and also 24-h urine was collected.
  
  
  RESULTS
  Absolute TEAC levels were higher 1 h after dinner with red wine compared with dinner with de-alcoholized red wine (1.3 versus 1.1 mmol Trolox equivalents/l; P = 0.03). Consumption of dinner together with de-alcoholized red wine acutely stimulated NF-κB activity in peripheral blood mononuclear cells (0.4-0.7 HeLa equivalents/2.5 μg protein; P = 0.006), whereas this increase was completely suppressed when the dinner was combined with red wine. A chronic increase in urinary 8-iso-PGF(2α) after 4 weeks of red wine consumption compared with de-alcoholized red wine consumption (157 pg/mg creatinine and 141 pg/mg creatinine, respectively, P = 0.006) was also observed.
  
  
  CONCLUSIONS
  Consumption of a moderate dose of red wine can acutely increase plasma TEAC and suppress NF-κB activation induced by a meal. Controversially, 4 weeks of red wine consumption compared with de-alcoholized red wine consumption increases the oxidative lipid damage marker 8-iso-PGF(2α).
authors.affiliation: TNO, Utrechtseweg 48, 3704 HE Zeist, The Netherlands. ilse.schrieks@tno.nl
authors.forename: Ilse C
authors.initials: IC
authors.lastname: Schrieks
authors.forename: Robin
authors.initials: R
authors.lastname: van den Berg
authors.forename: Aafje
authors.initials: A
authors.lastname: Sierksma
authors.forename: Joline W J
authors.initials: JW
authors.lastname: Beulens
authors.forename: Wouter H J
authors.initials: WH
authors.lastname: Vaes
authors.forename: Henk F J
authors.initials: HF
authors.lastname: Hendriks
mesh_terms: D000328:Adult; D000368:Aged; D000428:Alcohol Drinking / Q000097:blood* / Q000453:epidemiology; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D018592:Cross-Over Studies; D015536:Down-Regulation / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D018384:Oxidative Stress / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology; D014920:Wine
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016328:NF-kappa B