title: Microsatellite primers in Oenothera harringtonii (Onagraceae), an annual endemic to the shortgrass prairie of Colorado.
abstract: OBJECTIVE
  Microsatellite markers were developed in the annual herb, Oenothera harringtonii, to investigate patterns of genetic diversity, gene flow, and parentage within and among populations of this Colorado endemic.
  
  
  RESULTS
  Ten polymorphic loci were identified in O. harringtonii and tested in four populations sampled across the range of the species. These loci contained trinucleotide repeats with 7-29 alleles per locus. Nine of the 10 loci also amplified in O. caespitosa subsp. macroglottis, O. caespitosa subsp. marginata, and O. caespitosa subsp. navajoensis. In addition, we optimized three markers developed for O. biennis and provide reports of their effectiveness in all four taxa.
  
  
  CONCLUSIONS
  These results indicate the utility of these markers in O. harringtonii for future studies of genetic structure, gene flow, and parentage as well as their applicability in other members of the O. caespitosa species complex.
authors.affiliation: Plant Biology and Conservation, Chicago Botanic Garden, Glencoe, Illinois 60022, USA. kskogen@chicagobotanic.org
authors.forename: Krissa A
authors.initials: KA
authors.lastname: Skogen
authors.forename: Evan T
authors.initials: ET
authors.lastname: Hilpman
authors.forename: Sadie L
authors.initials: SL
authors.lastname: Todd
authors.forename: Jeremie B
authors.initials: JB
authors.lastname: Fant
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D051456:Gene Flow; D015723:Gene Library; D056426:Genetic Loci; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D031664:Oenothera / Q000145:classification / Q000235:genetics*; D018515:Plant Leaves / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers