title: Synthesis and in vitro evaluation of a biotinylated dextran-derived probe for molecular imaging.
abstract: Herein we report the design, synthesis, and in vitro evaluation of a gadolinium-containing biotinylated dextran-derived molecular imaging probe as a prospective neuroanatomical tracer by means of magnetic resonance imaging (MRI). The probe was effectively taken up by cultured differentiated murine neuroblastoma cells and significantly enhanced the contrast in T(1)- and T(2)-weighted MR images of labeled cells under physiological conditions. A significant longitudinal relaxation rate enhancement in the presence of avidin was observed allowing the verification of the results in the end of noninvasive longitudinal MRI connectivity studies by post-mortem histology. The in vitro results indicate that the probe has the potential to be used in vivo to identify the organization of global neuronal networks in the brain with MRI.
authors.affiliation: Department for Physiology of Cognitive Processes, Max-Planck Institute for Biological Cybernetics, Spemannstrasse 38, 72076 Tübingen, Germany. anurag.mishra@durham.ac.uk
authors.forename: Anurag
authors.initials: A
authors.lastname: Mishra
authors.forename: Rajendra
authors.initials: R
authors.lastname: Joshi
authors.forename: Jörn
authors.initials: J
authors.lastname: Engelmann
authors.forename: Nikos K
authors.initials: NK
authors.lastname: Logothetis
mesh_terms: D000818:Animals; D019921:Biotinylation / Q000379:methods*; D045744:Cell Line, Tumor; D003911:Dextrans / Q000032:analysis / Q000138:chemical synthesis*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005682:Gadolinium / Q000032:analysis / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D009447:Neuroblastoma / Q000175:diagnosis; D011849:Radioactive Tracers; D014407:Tumor Cells, Cultured
chemical_list: D003911:Dextrans; D011849:Radioactive Tracers; D005682:Gadolinium