title: [The effect of aminoguanidine on osteoclast in periodontal tissues during the rotation movement of teeth in rabbits].
abstract: OBJECTIVE
  To investigate the effect of aminoguanidine on osteoclast in periodontal tissues during experimental teeth movement in rabbits.
  
  
  METHODS
  36 male New Zealand white rabbits were divided randomly into two groups (18 rats in each group). The rabbits of the two groups received subperiosteal injections of aminoguanidine (as AG group) and normal saline (as NaCl group) locally at 2-day intervals respectively. Rabbits were sacrificed at 3,7,14,21,28,42 days after orthodontic force application.Immunohistochemistry and TRAP enzyme stain were used to detect the expression of iNOS and observe the osteoclasts. The data was analyzed with SPSS16.0 software package.
  
  
  RESULTS
  Collecting osteoclasts had the same change in all groups. From the 14th day till the end of the experiment, the AG group showed a significant decrease in the number of osteoclast (P<0.05)compared with the NaCl group. AG could effectively restrain the expression of iNOS in the pressure side after 21 days.The number of osteoclast was positively correlated with the proportion of iNOS in AG group (r=0.875,P<0.05).
  
  
  CONCLUSIONS
  AG could restrain the expression of iNOS, and affect the number of osteoclast by reducing the biosynthesis of NO in rabbits.
authors.affiliation: Department of Orthodontics, Suzhou Stomatology Hospital, Suzhou 215005, Jiangsu Province, China. rongsun486@163.com
authors.forename: Rong
authors.initials: R
authors.lastname: Sun
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Wu
mesh_terms: D000818:Animals; D006146:Guanidines; D008297:Male; D052247:Nitric Oxide Synthase Type II; D010010:Osteoclasts; D010519:Periodontium; D011817:Rabbits; D011897:Random Allocation; D012399:Rotation; D014087:Tooth Movement Techniques
chemical_list: D006146:Guanidines; D052247:Nitric Oxide Synthase Type II; C004479:pimagedine