title: Stress-response protein expression and DAF-16 translocation were induced in tributyltin-exposed Caenorhabditis elegans.
abstract: Exposure to tributyltin (TBT) with graded sublethal doses (0, 1, 10, 50 and 200 nM) resulted in the release of reactive oxygen species (ROS) and DNA damage in the nematode Caenorhabditis elegans. After the worms carrying transgenic reporters were exposed to TBT, the expressions of superoxide dismutase (SOD-3), glutathione S-transferase (GST-4) and heat shock proteins (HSP-4, HSP-16.2 and HSP-70) were semi-quantified after exposure. The results indicated that TBT caused dose-dependent induction of SOD-3, GST-4, HSP-4 and HSP-70. Furthermore, TBT exposure also induced DAF-16 translocation from cytoplasm to nucleus. The results implicated that C. elegans might be a potential animal model for TBT level monitoring and toxicity assessment.
authors.affiliation: Department of Life Sciences, Huainan Normal University, Huainan 232001, Anhui, China. yunwang2001@163.com
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Fenglei
authors.initials: F
authors.lastname: Jian
authors.forename: Jieyu
authors.initials: J
authors.lastname: Wu
authors.forename: Shunchang
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity*; D051858:Forkhead Transcription Factors; D015870:Gene Expression / Q000187:drug effects*; D005982:Glutathione Transferase / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014220:Trialkyltin Compounds / Q000633:toxicity*
chemical_list: D029742:Caenorhabditis elegans Proteins; D004785:Environmental Pollutants; D051858:Forkhead Transcription Factors; D017382:Reactive Oxygen Species; D014157:Transcription Factors; D014220:Trialkyltin Compounds; C108831:daf-16 protein, C elegans; C011559:tributyltin; D013482:Superoxide Dismutase; D005982:Glutathione Transferase