title: Tomographic imaging of ratiometric fluorescence resonance energy transfer in scattering media.
abstract: A method to visualize and quantify fluorescence resonance energy transfer (FRET) in scattering media is proposed. It combines the ratiometric FRET method with fluorescence molecular tomography (FMT) in continuous wave (CW) mode. To evaluate the performance of the proposed method, experiments on a tissue-mimicking phantom are carried out. The results demonstrate that the proposed approach is capable of visualizing and quantifying the FRET distribution in scattering media, which implies the further application of the ratiometric assay in in vivo studies.
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xu
authors.initials: X
authors.lastname: Cao
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Xu
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jianwen
authors.initials: J
authors.lastname: Luo
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
mesh_terms: D000465:Algorithms; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D049452:Green Fluorescent Proteins; D057809:HEK293 Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D055095:Optics and Photonics / Q000379:methods; D019047:Phantoms, Imaging; D010957:Plasmids; D014162:Transfection
chemical_list: D049452:Green Fluorescent Proteins