title: Increased levels of acute-phase inflammatory proteins in plasma of patients with sporadic CJD.
abstract: OBJECTIVE
  Screening plasma samples from patients with sporadic Creutzfeldt-Jakob disease (CJD) to discover diagnostic biomarkers.
  
  
  METHODS
  Plasma samples were collected from 17 patients with sporadic CJD, 17 patients with Alzheimer disease (AD), and 20 healthy subjects. A 2-phase screening was carried out using quantitative protein mass spectrometry. The putative sporadic CJD biomarkers were then validated independently by immunoturbidimetry.
  
  
  RESULTS
  Mass spectrometry uncovered 7 candidate sporadic CJD protein biomarkers, all belonging to the acute-phase response. Highly significant increases of these markers in patients with sporadic CJD, compared with healthy subjects and patients with AD, was confirmed by immunoturbidimetry.
  
  
  CONCLUSIONS
  The increase in plasma levels of a related set of acute-phase reactants in patients with sporadic CJD is a novel finding that suggests new pathogenetic hypotheses. The possible value of this set of proteins as biomarkers in the diagnosis of sporadic CJD or for blood/tissue donor screening remains to be further explored and validated in larger studies.
authors.affiliation: Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Fratini
authors.forename: Serena
authors.initials: S
authors.lastname: Principe
authors.forename: Maria
authors.initials: M
authors.lastname: Puopolo
authors.forename: Anna
authors.initials: A
authors.lastname: Ladogana
authors.forename: Anna
authors.initials: A
authors.lastname: Poleggi
authors.forename: Paola
authors.initials: P
authors.lastname: Piscopo
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Bruno
authors.forename: Silvano
authors.initials: S
authors.lastname: Castrechini
authors.forename: Roberto
authors.initials: R
authors.lastname: Pascone
authors.forename: Annamaria
authors.initials: A
authors.lastname: Confaloni
authors.forename: Luisa
authors.initials: L
authors.lastname: Minghetti
authors.forename: Franco
authors.initials: F
authors.lastname: Cardone
authors.forename: Maurizio
authors.initials: M
authors.lastname: Pocchiari
authors.forename: Marco
authors.initials: M
authors.lastname: Crescenzi
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism*; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood; D015415:Biomarkers / Q000097:blood; D007562:Creutzfeldt-Jakob Syndrome / Q000097:blood / Q000175:diagnosis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D000209:Acute-Phase Proteins; D015415:Biomarkers