title: Eastern blotting analysis and isolation of two new dammarane-type saponins from American ginseng.
abstract: Ginsenosides, the major active component of American ginseng, were analyzed using eastern blotting with anti-ginsenoside Rb(1) and Rg(1) monoclonal antibodies (MAbs). Immunoassay-guided fractionation of the methanol extract of American ginseng and column chromatography led to the isolation of two new minor dammarane-type saponins, named quinquenosides Ja (1) and Jb (2). Their structures were elucidated to be 6-O-[α-L-rhamnopyranosyl(1→2)-β-D-glucopyranosyl]-20-O-[β-D-glucopyranosyl(1→4)-β-D-glucopyranosyl]-3β,6α,12β,20β-tetrahydroxydammar-24-ene (1) and 3-O-[β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]-20-O-{[α-L-arabinofuranosyl(1→6)-β-D-glucopyranosyl](1→6)-β-D-glucopyranosyl}-3β,12β,20β-trihydroxydammar-24-ene (2) on the basis of chemical and spectroscopic methods.
authors.affiliation: Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan.
authors.forename: Nguyen Huu
authors.initials: NH
authors.lastname: Tung
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shoyama
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D036145:Ginsenosides / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D015151:Immunoblotting; D009682:Magnetic Resonance Spectroscopy; D005894:Panax / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D000097669:Dammaranes
chemical_list: D000911:Antibodies, Monoclonal; D036145:Ginsenosides; D014315:Triterpenes; D000097669:Dammaranes