title: Fibroblast growth factor-peptide promotes bone marrow recovery after irradiation.
abstract: Various members of the fibroblast growth factor (FGF) family mitigate radiation-induced damage. We designed and synthesized the binding domain peptide of FGF-2 (FGF-P) with a dimer form resistant to peptidase and examined its mitigatory effect on murine bone marrow cells. NIH Swiss mice were exposed to different doses of total body irradiation (TBI) and treated with ten doses of 5 mg/kg FGF-P. We achieved the following results: (1) FGF-P stimulated the growth of bone marrow cells harvested from mice exposed to 3 Gy; (2) on day 25 after 6 Gy TBI, the number of leukocytes and granulocytes was higher in the FGF-P group than in the vehicle-alone group; (3) FGF-P significantly increased the number of pro-B and pre-B cells; and (4) FGF-P treatment in vivo increased the long-term hematopoietic stem cells (LT-HSC) in bone marrow. These data reveal the underlying mechanism by which FGF-P rescued a significant percentage of the exposed mice. The increase of LT-HSC in bone marrow leads to a concomitant increase of pro-B and pre-B cells followed by leukocytes and granulocytes, which in turn enhance immunity against infection.
authors.affiliation: Institute of Digestive Diseases, Zhengzhou University, Henan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Laboratory Medicine, Kongjiang Hospital, Shanghai, China.
authors.forename: Yanqian
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Deping
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Chun
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Bingrong
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Zhenhuan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Shanmin
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Yansong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Okunieff
authors.affiliation: Department of Radiation Oncology, UF Shands Cancer Center, University of Florida, 100385, Gainesville, FL, 32610, USA. lurongzhang@ufl.edu.
authors.forename: Lurong
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D001853:Bone Marrow / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D008297:Male; D051379:Mice; D010446:Peptide Fragments / Q000494:pharmacology*; D014916:Whole-Body Irradiation
chemical_list: D010446:Peptide Fragments; D005346:Fibroblast Growth Factors