title: Determination of 10 ginsenosides in Panax ginseng of different harvest times based on HPLC fingerprints and principal component analysis.
abstract: A combinative method of HPLC fingerprinting and quantitative determination was successfully applied to monitor dynamic accumulation of ginsenosides in five-year-old Panax ginseng roots from different harvest times. The optimal chromatographic conditions were achieved on a C18 column with gradient elution using acetonitrile and 1 mmol · L(-1) KH2PO4 buffer solution at 203 nm. The result indicated that the contents of total ginsenosides showed significant variations, and a decrease tendency appeared in the growth period of the fifth year.
authors.affiliation: State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Chen
authors.forename: Jianguang
authors.initials: J
authors.lastname: Luo
authors.forename: Lingyi
authors.initials: L
authors.lastname: Kong
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000032:analysis* / Q000737:chemistry; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D025341:Principal Component Analysis; D015203:Reproducibility of Results
chemical_list: D000097:Acetonitriles; D036145:Ginsenosides; C032159:acetonitrile