title: Coagulation and survival in Drosophila melanogaster fondue mutants.
abstract: Drosophila larval coagulation factors have been identified in vitro. Better understanding of insect hemolymph coagulation calls for experiments in vivo. We have characterized a fondue (fon) mutation and null alleles isolated by imprecise excision of a transposable element. Loss of fon was pupal lethal, but adults could be recovered by expressing the UAS::fonGFP construct of Lindgren et al. (2008). Despite their lethality, fon mutations did not affect larval survival after wounding either when tested alone or in combination with a mutation in the hemolectin clotting factor gene. This reinforces the idea of redundant hemostatic mechanisms in Drosophila larvae, and independent pleiotropic functions of the fondue protein in coagulation and a vital process in metamorphosis.
authors.affiliation: Department of Biology, Illinois Institute of Technology, 3101 S Dearborn St., Chicago, IL 60616, USA.
authors.forename: Clara
authors.initials: C
authors.lastname: Bajzek
authors.forename: Amy M
authors.initials: AM
authors.lastname: Rice
authors.forename: Simonetta
authors.initials: S
authors.lastname: Andreazza
authors.forename: Mitchell S
authors.initials: MS
authors.lastname: Dushay
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics / Q000502:physiology*; D005260:Female; D006458:Hemolymph / Q000502:physiology*; D006487:Hemostasis; D007814:Larva / Q000502:physiology; D008297:Male; D009154:Mutation
chemical_list: D001798:Blood Proteins; D029721:Drosophila Proteins; C512855:fon protein, Drosophila