title: Site-specific labeling of his-tagged Nanobodies with ⁹⁹mTc: a practical guide.
abstract: 99mTc-tricarbonyl chemistry provides an elegant technology to site-specifically radiolabel histidine-tagged biomolecules. Considering their unique biochemical properties, this straightforward technology is particularly suited for Nanobodies. This chapter gives a detailed guide to generate highly specific Nanobody-derived radiotracers for both in vitro binding studies and in vivo molecular imaging.
authors.affiliation: In Vivo Cellular and Molecular Imaging (ICMI) Laboratory, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
authors.forename: Catarina
authors.initials: C
authors.lastname: Xavier
authors.forename: Nick
authors.initials: N
authors.lastname: Devoogdt
authors.forename: Sophie
authors.initials: S
authors.lastname: Hernot
authors.forename: Ilse
authors.initials: I
authors.lastname: Vaneycken
authors.forename: Matthias
authors.initials: M
authors.lastname: D'Huyvetter
authors.forename: Jens
authors.initials: J
authors.lastname: De Vos
authors.forename: Sam
authors.initials: S
authors.lastname: Massa
authors.forename: Tony
authors.initials: T
authors.lastname: Lahoutte
authors.forename: Vicky
authors.initials: V
authors.lastname: Caveliers
mesh_terms: D006639:Histidine / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods*; D061905:Single-Domain Antibodies / Q000737:chemistry*; D013667:Technetium / Q000737:chemistry*
chemical_list: D061905:Single-Domain Antibodies; D006639:Histidine; D013667:Technetium