title: [Effects of miR-590-5p on ox-LDL-induced endothelial cells apoptosis and LOX-1 expression].
abstract: OBJECTIVE
  To investigate the effect of miR-590-5p on the expression of lectin-like oxidized low density lipoprotein receptor 1 (LOX-1) in apoptotic human umbilical vein endothelial cells (HUVECs) induced by ox-LDL, and to explore the role of miR-590-5p in modulating HUVECs apoptosis.
  
  
  METHODS
  HUVECs were exposed to ox-LDL (50 μg/mL) for 0 to 48 h. Apoptosis was detected by Annexin V-FITC stain and was distinguished from necrosis by propidium iodide (PI) staining. The relative expression level of miR-590-5p in HUVECs was analyzed using real-time quantitative PCR (RT-qPCR). HUVECs were transfected with miR-590-5p mimics or miRNA mimics control followed by 50 μg/mL ox-LDL stimulation for 48 h. LOX-1 mRNA and protein were measured by RT-qPCR and Western blot, and apoptosis in HUVECs was analyzed by flow ctyometry after Annexin V-FITC/PI double stain.
  
  
  RESULTS
  Incubation of HUVECs with 50 μg/mL ox-LDL for 0 to 48 h resulted in a time-dependent induction of apoptotic cell death and down-regulation of miR-590-5p. Transfection of miR-590-5p mimics suppressed LOX-1 expression at both mRNA and protein levels, leading to a reduction of ox-LDL-induced apoptosis in HUVECs.
  
  
  CONCLUSIONS
  MiR-590-5p protects endothelial cells from ox-LDL induced apoptosis by inhibiting the expression of LOX-1.
authors.affiliation: Department of Neurology, Central South University, Changsha, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Qin
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
authors.forename: Ting
authors.initials: T
authors.lastname: Jiang
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051127:Scavenger Receptors, Class E / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D008077:Lipoproteins, LDL; C568221:MIRN590 microRNA, human; D035683:MicroRNAs; C495137:OLR1 protein, human; D012333:RNA, Messenger; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein