title: Bioproduction of Cinchona alkaloids by the endophytic fungus Diaporthe sp. associated with Cinchona ledgeriana.
abstract: We report that an endophytic filamentous fungus species of the genus Diaporthe isolated from Cinchona ledgeriana (Rubiaceae) produces Cinchona alkaloids (quinine, quinidine, cinchonidine, and cinchonine) upon cultivation in a synthetic liquid medium. This study provides evidence that Cinchona alkaloids are produced not only in Cinchona plant cells, but also in the endophytic microbe cells, and will help to elucidate the relationship between endophytic microbes and their host plants.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, 1 Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Maehara
authors.forename: Partomuan
authors.initials: P
authors.lastname: Simanjuntak
authors.forename: Chinami
authors.initials: C
authors.lastname: Kitamura
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Ohashi
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Shibuya
mesh_terms: D001203:Ascomycota / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D002930:Cinchona Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D019666:Rubiaceae / Q000382:microbiology*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D002930:Cinchona Alkaloids