title: Experimental validation of candidate schizophrenia gene ZNF804A as target for hsa-miR-137.
abstract: MicroRNAs (miRNAs) are small non-coding RNAs that mainly function as negative regulators of gene expression (Lai, 2002) and have been shown to be involved in schizophrenia etiology through genetic and expression studies (Burmistrova et al., 2007; Hansen et al., 2007a; Perkins et al., 2007; Beveridge et al., 2010; Kim et al., 2010). In a mega analysis of genome-wide association study (GWAS) of schizophrenia (SZ) and bipolar disorders (BP), a polymorphism (rs1625579) located in the primary transcript of a miRNA gene, hsa-miR-137, was reported to be strongly associated with SZ. Four SZ loci (CACNA1C, TCF4, CSMD1, C10orf26) achieving genome-wide significance in the same study were predicted and later experimentally validated (Kwon et al., 2011) as hsa-miR-137 targets. Here, using in silico, cellular and luciferase based approaches we also provide evidence that another well replicated candidate schizophrenia gene, ZNF804A, is also target for hsa-miR-137.
authors.affiliation: Virginia Institute for Psychiatric and Behavioral Genetics, Department of Psychiatry, Medical College of Virginia of Virginia Commonwealth University, Richmond, VA, USA.|Department of Molecular Human Genetics, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Albert H
authors.initials: AH
authors.lastname: Kim
authors.affiliation: Virginia Institute for Psychiatric and Behavioral Genetics, Department of Psychiatry, Medical College of Virginia of Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Erin K
authors.initials: EK
authors.lastname: Parker
authors.affiliation: Virginia Institute for Psychiatric and Behavioral Genetics, Department of Psychiatry, Medical College of Virginia of Virginia Commonwealth University, Richmond, VA, USA.|Department of Integrative Life Sciences, Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Vernell
authors.initials: V
authors.lastname: Williamson
authors.affiliation: Virginia Institute for Psychiatric and Behavioral Genetics, Department of Psychiatry, Medical College of Virginia of Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Gowon O
authors.initials: GO
authors.lastname: McMichael
authors.affiliation: Virginia Institute for Psychiatric and Behavioral Genetics, Department of Psychiatry, Medical College of Virginia of Virginia Commonwealth University, Richmond, VA, USA.|Mental Health Service Line, Washington VA Medical Center, Washington, DC, USA.|Department of Psychiatry, Georgetown University School of Medicine, Washington, DC, USA.|Keck School of Medicine of the University of Southern California, USA.
authors.forename: Ayman H
authors.initials: AH
authors.lastname: Fanous
authors.affiliation: Virginia Institute for Psychiatric and Behavioral Genetics, Department of Psychiatry, Medical College of Virginia of Virginia Commonwealth University, Richmond, VA, USA.|Center for Biomarker Research and Personalized Medicine, School of Pharmacy, Medical College of Virginia of Virginia Commonwealth University, Richmond, VA, USA.
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Vladimirov
mesh_terms: D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D016296:Mutagenesis / Q000502:physiology; D009447:Neuroblastoma / Q000473:pathology; D014162:Transfection
chemical_list: D051741:Kruppel-Like Transcription Factors; C545567:MIRN137 microRNA, human; D035683:MicroRNAs; C540527:ZNF804A protein, human; D008156:Luciferases