title: Field-amplified sample stacking-sweeping of vitamins B determination in capillary electrophoresis.
abstract: A capillary electrophoretic method for determination of five water soluble vitamins B along with baclofen as an internal standard has been developed and assessed in context of precision, accuracy, sensitivity, freedom from interference, linearity, detection and quantification limits. On-line preconcentration technique, namely field-amplified sample stacking (FASS)-sweeping, has been employed in respect to obtain more sensitive analysis. Separation conditions received after optimization procedure were as following background electrolyte (BGE), 10 mM NaH(2)PO(4), 80 mM SDS, (pH 7.25); sample matrix (SM), 10 mM NaH(2)PO(4) (pH 4.60); uncoated fused silica capillary (50 μm i.d. × 67 cm length); UV spectrophotometric detection at 200 nm; injection times: 10s and 30s at 3.45 kPa; applied voltage 22 kV; temperature 22°C. Validation parameters, namely precision, accuracy and linearity, were considered as satisfactory. Under the optimized conditions, it has been also successfully applied for vitamins B determination in bacterial growth medium and commercially available Ilex paraguariensis leaves.
authors.affiliation: Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Gdańsk, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Dziomba
authors.forename: Piotr
authors.initials: P
authors.lastname: Kowalski
authors.forename: Tomasz
authors.initials: T
authors.lastname: Bączek
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D030017:Ilex / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis*; D014803:Vitamin B Complex / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D014803:Vitamin B Complex