title: Neuroprotective effect of quercetin on the duodenum enteric nervous system of streptozotocin-induced diabetic rats.
abstract: BACKGROUND
  In diabetes mellitus (DM), hyperglycemia promotes changes in biochemical mechanisms that induce oxidative stress. Oxidative stress has been closely linked to adverse consequences that affect the function of the gastrointestinal tract caused by injuries to the enteric nervous system (ENS) that in turn cause neurodegeneration and enteric glial loss. Therapeutic approaches have shown that diet supplementation with antioxidants, such as quercetin, reduce oxidative stress.
  
  
  OBJECTIVE
  This work sought to evaluate neurons and enteric glial cells in the myenteric and submucosal plexuses of the duodenum in diabetic rats supplemented with quercetin.
  
  
  METHODS
  The duodenum of 24 rats, including a control group (C), control quercetin supplementation group (CQ), diabetic group (D), and diabetic quercetin supplementation group (DQ), were used to investigate whole mounts of muscular and submucosal layers subjected to immunohistochemistry to detect vasoactive intestinal peptide in the myenteric layer and double-staining for HuC-D/neuronal nitric oxide synthase (nNOS) and HuC-D/S100.
  
  
  RESULTS
  A reduction of the general neuronal population (HuC/D) was found in the myenteric and submucosal plexuses (p < 0.001) in the D and DQ groups. The nitrergic subpopulation (nNOS) decreased only in the myenteric plexus (p < 0.001), and glial cells decreased in both plexuses (p < 0.001) in the D and DQ groups. In diabetic rats, quercetin supplementation reduced neuronal and glial loss. Diabetes promoted an increase in the cell body area of both the general and nitrergic populations. Quercetin supplementation only prevented neuronal hypertrophy in the general population.
  
  
  CONCLUSIONS
  Supplementation with quercetin eased the damage caused by diabetes, promoting a neuroprotective effect and reducing enteric glial loss in the duodenum.
authors.affiliation: Department of Morphological Sciences, Universidade Estadual de Maringá, Av. Colombo, 5790 Bloco H-79, Maringá, PR, CEP 87020-900, Brazil.
authors.forename: Cláudia Regina Pinheiro
authors.initials: CR
authors.lastname: Lopes
authors.forename: Paulo Emílio Botura
authors.initials: PE
authors.lastname: Ferreira
authors.forename: Jacqueline Nelisis
authors.initials: JN
authors.lastname: Zanoni
authors.forename: Angela Maria Pereira
authors.initials: AM
authors.lastname: Alves
authors.forename: Eder Paulo Belato
authors.initials: EP
authors.lastname: Alves
authors.forename: Nilza Cristina
authors.initials: NC
authors.lastname: Buttow
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D004386:Duodenum / Q000294:innervation*; D017615:Enteric Nervous System / Q000187:drug effects*; D005260:Female; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D013368:Submucous Plexus / Q000166:cytology / Q000187:drug effects
chemical_list: D018696:Neuroprotective Agents; D013311:Streptozocin; D011794:Quercetin