title: Further insights about the beneficial effects of n-3 fatty acids in the early molecular events of renal fibrosis in vitro.
abstract: BACKGROUND
  Accumulating experimental and clinical evidence reveals beneficial effects of n-3 polyunsaturated fatty acids (PUFAs) in kidney disease by modulating inflammation and fibrosis mechanisms that lead to renal failure.
  
  
  METHODS
  EPA, DHA (n-3 PUFAs) and AA (n-6 PUFA) effects, compared to those of AngII, on renal fibrotic processes at the extracellular matrix (ECM) level were verified in human mesangial cells in vitro, by means of RT-PCR, mitogenic assay and Western-blot analysis.
  
  
  RESULTS
  Unlike AngII, EPA and DHA enhanced the expression of MMP2 and DN, a TGFbeta inhibitor, while decreasing mitogenic factors such as PDGF and bFGF, and cell proliferation. Moreover, n-3 PUFAs elicited Bax expression in AngII-treated cells and downregulated COX-2--an enzyme involved in the inflammatory cascade. The mechanism of action could implicate PPARgamma activation, as this transcription factor was shown to translocate to the nucleus upon n-3 PUFA treatment.
  
  
  CONCLUSIONS
  These results complement our previous reports demonstrating that EPA and DHA prevent ECM accumulation and inflammation that typify the fibrotic process, providing new insights into the cellular and molecular mechanisms underlying their beneficial effects. We confirm that n-3 PUFAs could effectively counteract kidney fibrosis development providing a rationale for their use in clinical settings.
authors.affiliation: Nephrology Unit, Department of Medicine DIMED, University of Padova, Padova, Italy. giovanna.priante@unipd.it
authors.forename: Giovanna
authors.initials: G
authors.lastname: Priante
authors.forename: Estella
authors.initials: E
authors.lastname: Musacchio
authors.forename: Chiara
authors.initials: C
authors.lastname: Valvason
authors.forename: Giulio
authors.initials: G
authors.lastname: Clari
authors.forename: Luciana
authors.initials: L
authors.lastname: Bordin
authors.forename: Leonardo
authors.initials: L
authors.lastname: Sartori
authors.forename: Bruno
authors.initials: B
authors.lastname: Baggio
mesh_terms: D002478:Cells, Cultured; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use*; D005355:Fibrosis / Q000517:prevention & control; D006801:Humans; D007668:Kidney / Q000473:pathology*
chemical_list: D015525:Fatty Acids, Omega-3