title: Induced mitotic  homologous recombination by the babesicide imidocarb dipropionate in Aspergillus  nidulans diploid cells.
abstract: Imidocarb  dipropionate (IMD) is a chemotherapeutic agent prescribed for the treatment and  control of babesiosis; it is known to be a nucleic acid synthesis inhibitor.  Although it is an effective babesicide, there are reports of persistent IMD  residues retained at high levels in edible tissues of cattle, swine and sheep,  raising concerns about potential effects on humans. Since the carcinogenic  potential of a chemical compound can be assessed through its effect on the  homologous recombination, we investigated whether IMD is recombinogenic in Aspergillus  nidulans diploid cells and whether it is capable of inducing homozygosis in  genes that were previously heterozygous. This analysis was done with a  homozygotization assay applied to a heterozygous diploid strain of A.  nidulans. IMD used at non-toxic concentrations (2.5 to 10.0 μM) was  recombinogenic, demonstrated by homozygotization indices higher than 2.0 for  diploid markers. A diploid homozygous for genetic markers from chromosomes I  and II was also produced. Since DNA replication blockers that induce DNA strand  breaks have been classified as potent inducers of homologous recombination, the  recombinogenic potential of IMD may be due to induction of recombinational  repair.
authors.affiliation: Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, Maringá, PR, Brasil.
authors.forename: P A S R
authors.initials: PA
authors.lastname: Santos
authors.forename: J R
authors.initials: JR
authors.lastname: Sant'Anna
authors.forename: C C S
authors.initials: CC
authors.lastname: Franco
authors.forename: L J
authors.initials: LJ
authors.lastname: Rosada
authors.forename: G N M
authors.initials: GN
authors.lastname: Esquissato
authors.forename: M A A
authors.initials: MA
authors.lastname: Castro-Prado
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D001233:Aspergillus nidulans / Q000166:cytology* / Q000187:drug effects / Q000235:genetics*; D001403:Babesia / Q000187:drug effects; D002417:Cattle; D015825:Chromosomes, Fungal / Q000235:genetics; D003434:Crossing Over, Genetic / Q000187:drug effects; D004171:Diploidy; D005838:Genotype; D007095:Imidocarb / Q000031:analogs & derivatives* / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects*; D011995:Recombination, Genetic / Q000187:drug effects*
chemical_list: D000981:Antiprotozoal Agents; D007095:Imidocarb; C031719:imidocarb dipropionate