title: Controlled immobilisation of active enzymes on the cowpea mosaic virus capsid.
abstract: Immobilisation of horseradish peroxidase (HRP) and glucose oxidase (GOX) via covalent attachment of modified enzyme carbohydrate to the exterior of the cowpea mosaic virus (CPMV) capsid gave high retention of enzymatic activity. The number of enzymes bound per virus was determined to be about eleven for HRP and 2-3 for GOX. This illustrates that relatively large biomacromolecules can be readily coupled to the virus surface using simple conjugation strategies. Virus-biomacromolecule hybrids have great potential for uses in catalysis, diagnostic assays or biosensors.
authors.affiliation: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
authors.forename: Alaa A A
authors.initials: AA
authors.lastname: Aljabali
authors.forename: J Elaine
authors.initials: JE
authors.lastname: Barclay
authors.forename: Nicole F
authors.initials: NF
authors.lastname: Steinmetz
authors.forename: George P
authors.initials: GP
authors.lastname: Lomonossoff
authors.forename: David J
authors.initials: DJ
authors.lastname: Evans
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques; D036022:Capsid Proteins / Q000737:chemistry* / Q000378:metabolism; D017798:Comovirus / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry
chemical_list: D036022:Capsid Proteins; D004800:Enzymes, Immobilized; D010504:Periodic Acid; C009288:metaperiodate; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase