title: Structured oblique illumination microscopy for enhanced resolution imaging of non-fluorescent, coherently scattering samples.
abstract: Many biological structures of interest are beyond the diffraction limit of conventional microscopes and their visualization requires application of super-resolution techniques. Such techniques have found remarkable success in surpassing the diffraction limit to achieve sub-diffraction limited resolution; however, they are predominantly limited to fluorescent samples. Here, we introduce a non-fluorescent analogue to structured illumination microscopy, termed structured oblique illumination microscopy (SOIM), where we use simultaneous oblique illuminations of the sample to multiplex high spatial-frequency content into the frequency support of the system. We introduce a theoretical framework describing how to demodulate this multiplexed information to reconstruct an image with a spatial-frequency support exceeding that of the system's classical diffraction limit. This approach allows enhanced-resolution imaging of non-fluorescent samples. Experimental confirmation of the approach is obtained in a reflection test target with moderate numerical aperture.
authors.affiliation: Department of Biomedical Engineering, Fitzpatrick Institute for Photonics, Duke University, 136 Hudson Hall, Durham NC 27708,  USA.
authors.forename: Shwetadwip
authors.initials: S
authors.lastname: Chowdhury
authors.forename: Al-Hafeez
authors.initials: AH
authors.lastname: Dhalla
authors.forename: Joseph
authors.initials: J
authors.lastname: Izatt