title: Complete genomic sequence of the virulent bacteriophage RAP44 of Riemerella anatipestifer.
abstract: A virulent Riemerella anatipestifer bacteriophage, RAP44, belonging to the Siphoviridae family of tailed phages, was previously isolated from feces of healthy Muscovy ducks in China. A complete genomic sequence analysis indicates that the phage's genome consists of a linear, double-stranded DNA molecule of 49,329 nucleotides. Eighty open reading frames (ORF) were identified. Putative functions could be assigned to 24 of the ORFs. The location of these genes was consistent with organization of the genome in a modular format which includes modules for host cell lysis, tail morphogenesis, head morphogenesis, and DNA replication and modification modules. Until now, no R. anatipestifer phage genome sequence has been reported in the literature. Therefore, this study represents the first complete genomic and molecular description of the R. anatipestifer phage.
authors.affiliation: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, People's Republic of China.
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Cheng
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Chen
authors.forename: Teng
authors.initials: T
authors.lastname: Zheng
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Fu
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Shi
authors.forename: Chun-He
authors.initials: CH
authors.lastname: Wan
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D004261:DNA Replication; D004372:Ducks / Q000382:microbiology; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction; D060494:Riemerella / Q000821:virology*; D017906:Siphoviridae / Q000145:classification* / Q000235:genetics*; D019065:Virus Assembly / Q000235:genetics