title: On the use of ultracentrifugal devices for sedimented solute NMR.
abstract: We have recently proposed sedimented solute NMR (SedNMR) as a solid-state method to access biomolecules without the need of crystallization or other sample manipulation. The drawback of SedNMR is that samples are intrinsically diluted and this is detrimental for the signal intensity. Ultracentrifugal devices can be used to increase the amount of sample inside the rotor, overcoming the intrinsic sensitivity limitation of the method. We designed two different devices and we here report the directions for using such devices and the relevant equations for determining the parameters for sedimentation.
authors.affiliation: Center for Magnetic Resonance (CERM), University of Florence, Via L. Sacconi 6, 50019 Sesto Fiorentino, FI, Italy. ivanobertini@cerm.unifi.it
authors.forename: Ivano
authors.initials: I
authors.lastname: Bertini
authors.forename: Frank
authors.initials: F
authors.lastname: Engelke
authors.forename: Leonardo
authors.initials: L
authors.lastname: Gonnelli
authors.forename: Benno
authors.initials: B
authors.lastname: Knott
authors.forename: Claudio
authors.initials: C
authors.lastname: Luchinat
authors.forename: David
authors.initials: D
authors.lastname: Osen
authors.forename: Enrico
authors.initials: E
authors.lastname: Ravera
mesh_terms: D003460:Crystallization; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D014461:Ultracentrifugation / Q000379:methods*
chemical_list: D011506:Proteins; D012996:Solutions