title: Platelet aggregation and anti-inflammatory effects of garden pea, Desi chickpea and Kabuli chickpea.
abstract: Inflammation is the natural body defense mechanism for the removal of injurious agents, necrosed cells and tissues from the body. This study was aimed to evaluate the anti-inflammatory and platelet aggregation effects of three medicinal plants of Pakistan. Methanolic extract of garden pea inhibited arachidonic acid (AA)-induced platelet aggregation (IC50 = 35 microg/mL) and platelet activating factor (PAF)-induced platelet aggregation (IC50 = 38 microg/mL) in a dose dependent fashion. Methanolic extract of Desi chickpea inhibited arachidonic acid (AA) induced platelet aggregation (IC50 value = AA = 46 microg/mL) in dose dependent fashion while was found not active against PAF-induced platelet aggregation. Methanolic extract of Kabuli chickpea was found not active against both arachidonic acid (AA)-induced platelet aggregation and PAF-induced platelet aggregation. The best potential to inhibit in vitro COX-2 activity showed garden pea (Pisum sativum: the synthesis of PGE2 reduced by 92% in comparison with untreated control wells) followed by Desi chickpea (Cicer arietinum var; 87% inhibition) and Kabuli chickpea extracts (Cicer arietinum var: 65% inhibition). All extracts were tested at concentration 20 microg/mL. in COX-2 assay. The results indicate that if the same were happening in vito, Garden pea, Desi chickpea and Kabuli chickpea could be useful as natural antithrombotic anti-inflammatory materials.
authors.affiliation: Research Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Karachi, Karachi-75270, Pakistan.
authors.forename: M
authors.initials: M
authors.lastname: Zia-ul-Haq
authors.forename: Barkat Ali
authors.initials: BA
authors.lastname: Khan
authors.forename: Premysl
authors.initials: P
authors.lastname: Landa
authors.forename: Zsófia
authors.initials: Z
authors.lastname: Kutil
authors.forename: Sagheer
authors.initials: S
authors.lastname: Ahmed
authors.forename: Mughal
authors.initials: M
authors.lastname: Qayum
authors.forename: Shakeel
authors.initials: S
authors.lastname: Ahmad
mesh_terms: D016718:Arachidonic Acid; D029863:Cicer / Q000737:chemistry; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D000432:Methanol / Q000737:chemistry; D018532:Pisum sativum / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010972:Platelet Activating Factor; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010979:Platelet Function Tests; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D012639:Seeds; D012997:Solvents / Q000737:chemistry; D013997:Time Factors
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D010936:Plant Extracts; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D011994:Recombinant Proteins; D012997:Solvents; D016718:Arachidonic Acid; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D015232:Dinoprostone; D007213:Indomethacin; D000432:Methanol