title: Distribution of methanotrophs in the phyllosphere.
abstract: Plants have been reported to emit methane as well as methanol originating in their cell-wall constituents. We investigated methanotrophs in the phyllosphere by the enrichment culture method with methane as sole carbon source. We enriched methanotrophs from the leaves, flowers, bark, and roots of various plants. Analysis of the pmoA and mxaF genes retrieved from the enrichment cultures revealed that methanotrophs closely related to the genera Methylomonas, Methylosinus, and Methylocystis inhabit not only the rhizosphere but also the phyllosphere, together with methanol-utilizing bacteria.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Kyoto 606-8502, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iguchi
authors.forename: Izuru
authors.initials: I
authors.lastname: Sato
authors.forename: Maiko
authors.initials: M
authors.lastname: Sakakibara
authors.forename: Hiroya
authors.initials: H
authors.lastname: Yurimoto
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Sakai
mesh_terms: D003470:Culture Media; D035264:Flowers / Q000382:microbiology; D005798:Genes, Bacterial; D008697:Methane / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism; D042083:Methylocystaceae / Q000145:classification / Q000235:genetics*; D020620:Methylomonas / Q000145:classification / Q000235:genetics*; D020581:Methylosinus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D016133:Polymerase Chain Reaction
chemical_list: D003470:Culture Media; D008697:Methane; D000432:Methanol