title: [Primary drug resistance of HIV-1 infected men who have sex with men in Shenzhen, China].
abstract: OBJECTIVE
  To evaluate the status of drug resistance among treat-naive HIV-1 infected men who have sex with men (MSM) in Shenzhen during the period of 2008 - 2010.
  
  
  METHODS
  Plasma samples of 227 treatment-naive HIV-1 infected MSM were collected in Shenzhen. HIV-1 pol genes (RT and PR) were amplified by nested-polymerase chain reaction (PCR) from RNA. Phylogenetic and drug resistance analyses were performed on the nucleotide sequence data.
  
  
  RESULTS
  A total of 164 pol gene sequences were amplified. The prevalence of primary genotypic drug resistance was 14.6%. The overall prevalence of drug-resistant mutations was 22.6%, corresponding to 8.54% for protease inhibitors (PI) minor drug resistance mutation, 1.22% for nucleotide reverse transcriptase inhibitors (NRTI) drug resistance mutation and 13.41% for non-nucleotide reverse transcriptase inhibitors (NNRTI) drug resistance mutation. The prevalence of drug-resistant mutations was 30.88% for CRF01_AE strain and 19.23% for B strain.
  
  
  CONCLUSIONS
  The prevalence of drug resistance is relatively moderate in the treat-naive HIV-1 infected MSM in Shenzhen. The prevalence of drug-resistant HIV-1 among MSM in Shenzhen should raise a high alert.
authors.affiliation: Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China.
authors.forename: Guang-lu
authors.initials: GL
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.forename: Yu-mao
authors.initials: YM
authors.lastname: Cai
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Fu-chang
authors.initials: FC
authors.lastname: Hong
authors.forename: Wei-na
authors.initials: WN
authors.lastname: Jiang
authors.forename: Yong-lai
authors.initials: YL
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Peng
authors.forename: Chun-lai
authors.initials: CL
authors.lastname: Zhang
authors.forename: Juan-juan
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Tie-jian
authors.initials: TJ
authors.lastname: Feng
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000453:epidemiology / Q000821:virology*; D000328:Adult; D002681:China / Q000453:epidemiology; D024882:Drug Resistance, Viral; D015497:HIV-1 / Q000187:drug effects*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D012367:RNA, Viral
chemical_list: D012367:RNA, Viral