title: Identification of a novel Baeyer-Villiger monooxygenase from Acinetobacter radioresistens: close relationship to the Mycobacterium tuberculosis prodrug activator EtaA.
abstract: This work demonstrates that Acinetobacter radioresistens strain S13 during the growth on medium supplemented with long-chain alkanes as the sole energy source expresses almA gene coding for a Baeyer-Villiger monooxygenase (BVMO) involved in alkanes subterminal oxidation. Phylogenetic analysis placed the sequence of this novel BVMO in the same clade of the prodrug activator ethionamide monooxygenase (EtaA) and it bears only a distant relation to the other known class I BVMO proteins. In silico analysis of the 3D model of the S13 BVMO generated by homology modelling also supports the similarities with EtaA by binding ethionamide to the active site. In vitro experiments carried out with the purified enzyme confirm that this novel BVMO is indeed capable of typical Baeyer-Villiger reactions as well as oxidation of the prodrug ethionamide.
authors.affiliation: Department of Life Sciences and Systems Biology, University of Torino, via Accademia Albertina 13, 10123 Torino, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Minerdi
authors.forename: Ivan
authors.initials: I
authors.lastname: Zgrablic
authors.forename: Sheila J
authors.initials: SJ
authors.lastname: Sadeghi
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Gilardi
mesh_terms: D000150:Acinetobacter / Q000201:enzymology* / Q000235:genetics; D000473:Alkanes / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004734:Energy Metabolism; D005000:Ethionamide / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D011355:Prodrugs / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D000473:Alkanes; D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D011355:Prodrugs; C414178:etaA protein, Mycobacterium tuberculosis; D006899:Mixed Function Oxygenases; D010105:Oxygenases; D005000:Ethionamide