title: [Expression of microRNA-183 in stage II ( gastric cancer and its association with Ezrin protein].
abstract: OBJECTIVE
  To investigate the expression of microRNA-183 (miR-183) and Ezrin protein in stage II( gastric cancer (GC).
  
  
  METHODS
  Specimens of stage II( GC and paracancer tissues (5 cm away from the tumor tissues) were collected from 72 patients. Real-time PCR was used to detect the miR-183 expression. Immunohistochemistry was used to examine the Ezrin protein expression in the tumor tissue. The associations of miR-183 expression with the clinicopathologic features of stage II( GC and Ezrin expression were analyzed.
  
  
  RESULTS
  miR-183 expression was lower in stage II( gastric cancer tissues compared with the paracancer tissues samples(median relative expression, 0.676 vs. 1.000, P<0.05). Low expression of miR-183 was significantly associated with histological differentiation(0.429 vs. 0.907, P<0.05), lymph node metastasis(0.507 vs. 0.908, P<0.05). The survival was shorter in patient with low expression of miR-183(63.0±4.0) as compared to those with high expression of miR-183(75.2±3.8)(P<0.05). There was a negative correlation between the expression of miR-183 and Ezrin(r=-0.272, P<0.05).
  
  
  CONCLUSIONS
  miR-183 is down-regulated in stage II( GC, and associated with the differentiation, metastasis, and prognosis. Ezrin is a potential regulatory protein of miR-183.
authors.affiliation: Department of Gastric Surgery, Fujian Medical University, Fuzhou, China.
authors.forename: Wei-wen
authors.initials: WW
authors.lastname: Zheng
authors.forename: Chang-ming
authors.initials: CM
authors.lastname: Huang
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Xie
authors.forename: Chao-hui
authors.initials: CH
authors.lastname: Zheng
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Jia-bin
authors.initials: JB
authors.lastname: Wang
authors.forename: Jian-xian
authors.initials: JX
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D035683:MicroRNAs; D000099149:Ezrin; C533449:MIRN183 microRNA, human