title: [Analysis of liposoluble constituents in Holotrichia diomphalia by GC-MS and investigation their anti-inflammatory and analgesic activities].
abstract: OBJECTIVE
  To analysis liposoluble constituents of Holotrichia diomphalia by GC-MS and measure their anti-inflammatory and analgesic activities.
  
  
  METHODS
  The composition of liposoluble constituents were determined by GC-MS. The dimethylbenzene-induced mice inflammatory models were established. The pain models were obtained by hot plate and acetic acid in mice.
  
  
  RESULTS
  Twenty-two components were identified from the petroleum ether extract of Holotrichia diomphalia. The major components were oleic acid, palmitic acid and palmitoleic acid. The petroleum ether extract was able to significantly inhibit the mice ear edema induced by dimethyl-benzene. The pain in mice caused by acetic acid and hot plate were evidently suppressed by the petroleum ether extract.
  
  
  CONCLUSIONS
  The petroleum ether extract of Holotrichia diomphalia has obvious anti-inflammation and analgesic effects.
authors.affiliation: Medicine College of Shaanxi University of Chinese Medicine, Xianyang 712046, China. pkmc123@163.com
authors.forename: Ke
authors.initials: K
authors.lastname: Pei
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Guo
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Xie
authors.forename: Zhong-Min
authors.initials: ZM
authors.lastname: He
authors.forename: Si-Wang
authors.initials: SW
authors.lastname: Wang
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D001517:Coleoptera / Q000737:chemistry; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D005227:Fatty Acids / Q000032:analysis* / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006358:Hot Temperature; D008297:Male; D008419:Materia Medica / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D010146:Pain / Q000517:prevention & control; D010147:Pain Measurement / Q000187:drug effects; D012995:Solubility
chemical_list: D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005227:Fatty Acids; D008419:Materia Medica