title: Axenic cultivation and comparative phospholipase A2 activity of Giardia duodenalis in a serum-free medium.
abstract: Mammalian serum is essential for the growth of Giardia duodenalis cultivated under axenic conditions. Unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. TYI-33-PACSR is a TYI medium supplemented with a serum replacement (PACSR) made up of Earle's amino acid solution, Diamond's vitamin-tween 80 mixtures and LCR (a lipid-cholesterol - rich mixture). PACSR was previously used in the culture media for axenic cultivation of Entamoeba histolytica and Trichomonas vaginalis. The main objective of this work was to demonstrate that TYI-33-PACSR is useful for axenic cultivation of G. duodenalis. Additionally, the activity of phospholipase A(2) (PLA A(2)) in the sub-cellular vesicular fraction (P30) of G. duodenalis grown in TYI-S-33 and TYI-33-PACSR was compared. All strains of Giardia grown in TYI-33-PACSR reached relative cellular densities of 91 to 95% compared to controls growing in serum-supplemented TYI-S-33 medium. Additionally, PLA A(2) activity was similar in the P30 sub-cellular fraction obtained from trophozoites growing in TYI-S-33 and TYI-33-PACSR. Thus, TYI-33-PACSR could be useful in analyzing the biological properties of G. duodenalis in the absence of serum.
authors.affiliation: Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, México.
authors.forename: Benito David
authors.initials: BD
authors.lastname: Mata-Cárdenas
authors.forename: Magda Elizabeth
authors.initials: ME
authors.lastname: Hernández-García
authors.forename: Francisco
authors.initials: F
authors.lastname: González-Salazar
authors.forename: Jesús Norberto
authors.initials: JN
authors.lastname: Garza-González
authors.forename: Rebeca
authors.initials: R
authors.lastname: Palacios-Corona
authors.forename: Elva I
authors.initials: EI
authors.lastname: Cortés-Gutiérrez
authors.forename: Javier
authors.initials: J
authors.lastname: Vargas-Villarreal
mesh_terms: D000818:Animals; D016895:Culture Media, Serum-Free / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D016829:Giardia lamblia / Q000201:enzymology* / Q000378:metabolism*; D054467:Phospholipases A2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D016895:Culture Media, Serum-Free; D054467:Phospholipases A2