title: Expression of a Trichoderma reesei β-1,4 endo-xylanase in tall fescue modifies cell wall structure and digestibility and elicits pathogen defence responses.
abstract: An endo-xylanase from Trichoderma reesei (xyn2) has been expressed in tall fescue targeted to the vacuole, apoplast or Golgi, constitutively under the control of the rice actin promoter, and to the apoplast under the control of a senescence enhanced gene promoter. Constitutive xylanase expression in the vacuole, apoplast, and golgi, resulted in only a small number of plants with low enzyme activities and in reduced plant growth in apoplast, and golgi targeted plants. Constitutive expression in the apoplast also resulted in increased levels of cell wall bound hydroxycinnamic acid monomers and dimers, but no significant effect on cell wall xylose or arabinose content. In situ constitutive xylanase expression in the Golgi also resulted in increased ferulate dimers. However, senescence induced xylanase expression in the apoplast was considerably higher and did not affect plant growth or the level of monomeric hydroxycinnamic acids or lignin in the cell walls. These plants also showed increased levels of ferulate dimers, and decreased levels of xylose with increased levels of arabinose in their cell walls. While the release of cell wall hydroxycinnamic acids on self digestion was enhanced in these plants in the presence of exogenously applied ferulic acid esterase, changes in cell wall composition resulted in decreases in both tissue digestibility and cellulase mediated sugar release. In situ detection of H(2)O(2) production mediated by ethylene release in leaves of plants expressing apoplast xylanase could be leading to increased dimerisation. High-level xylanase expression in the apoplast also resulted in necrotic lesions on the leaves. Together these results indicate that xylanase expression in tall fescue may be triggering plant defence responses analogous to foliar pathogen attack mediated by ethylene and H(2)O(2).
authors.affiliation: Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, University Park, PA 16802, USA. mmb26@psu.edu
authors.forename: Marcia M de O
authors.initials: MM
authors.lastname: Buanafina
authors.forename: Tim
authors.initials: T
authors.lastname: Langdon
authors.forename: Sue
authors.initials: S
authors.lastname: Dalton
authors.forename: Phillip
authors.initials: P
authors.lastname: Morris
mesh_terms: D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000235:genetics* / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism; D031751:Festuca / Q000737:chemistry / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D054884:Host-Pathogen Interactions; D006861:Hydrogen Peroxide / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D010935:Plant Diseases / Q000276:immunology*; D010936:Plant Extracts / Q000737:chemistry; D057865:Plant Immunity; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014242:Trichoderma / Q000235:genetics*; D014990:Xylans / Q000378:metabolism
chemical_list: D003373:Coumaric Acids; D005030:Ethylenes; D010936:Plant Extracts; D010940:Plant Proteins; D014990:Xylans; D008031:Lignin; C085118:arabinoxylan; C036216:ethylene; C004999:ferulic acid; D006861:Hydrogen Peroxide; D043364:Endo-1,4-beta Xylanases