title: Redox instability and hemin loss of mutant sperm whale myoglobins induced by 4-hydroxynonenal in vitro.
abstract: The effects of 4-hydroxy-2-nonenal (HNE) on redox stability of Oxy- and Deoxy- wild-type (WT) and recombinant sperm whale myoglobins (P88H/Q152H, L29F, H97A, and H64F) and hemin loss from Met-myoglobin (Mb) were investigated. HNE induced greater redox instability in WT and mutant Mbs compared to controls (p < 0.05). The extent of HNE-induced OxyMb oxidation was lesser in L29F (p < 0.05) and greater in H97A and P88H/Q152H than in WT (p < 0.05). H64F DeoxyMb was more redox stable than WT DeoxyMb in the presence of HNE (p < 0.05). HNE alkylation occurred exclusively on histidine residues, and histidine 48 was alkylated in all sperm whale myoglobins. HNE alkylation accelerated the protoporphyrin moiety loss only in H97A. Met- forms of WT and L29F but not Deoxy- or Oxy- forms released hemin during storage. Primary structure strongly influenced Mb redox stability in the presence of reactive secondary lipid oxidation products.
authors.affiliation: Department of Animal Science, University of Connecticut , Storrs, Connecticut 06269, United States.
authors.forename: Nantawat
authors.initials: N
authors.lastname: Tatiyaborworntham
authors.forename: Cameron
authors.initials: C
authors.lastname: Faustman
authors.forename: Shuang
authors.initials: S
authors.lastname: Yin
authors.forename: Ranjith
authors.initials: R
authors.lastname: Ramanathan
authors.forename: Richard A
authors.initials: RA
authors.lastname: Mancini
authors.forename: Surendranath P
authors.initials: SP
authors.lastname: Suman
authors.forename: Carol M
authors.initials: CM
authors.lastname: Beach
authors.forename: Naveena B
authors.initials: NB
authors.lastname: Maheswarappa
authors.forename: Eric W
authors.initials: EW
authors.lastname: Grunwald
authors.forename: Mark P
authors.initials: MP
authors.lastname: Richards
mesh_terms: D000447:Aldehydes / Q000737:chemistry* / Q000494:pharmacology*; D000478:Alkylation; D000818:Animals; D006427:Hemin / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D009154:Mutation; D009211:Myoglobin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D055550:Protein Stability; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D050661:Sperm Whale
chemical_list: D000447:Aldehydes; D006657:Histones; D009211:Myoglobin; D011994:Recombinant Proteins; D006639:Histidine; D006427:Hemin; C027576:4-hydroxy-2-nonenal