title: Platelet glycoprotein IIb HPA-3 a/b polymorphism is associated with native arteriovenous fistula thrombosis in chronic hemodialysis patients.
abstract: OBJECTIVE
  The aim of this study was to investigate the association of Glycoprotein IIb (GPIIb) human platelet antigen-3 (HPA-3) a/b polymorphism with end-stage renal disease (ESRD) on hemodialysis (HD) and native Arteriovenous fistula (AVF) thrombosis.
  
  
  METHODS
  The polymorphism in the GPIIb subunit of the receptor HPA-3 (a and b alleles) was identified by polymerase chain reaction with sequence-specific primers (PCR-SSP) in 145 HD patients and 120 healthy controls from a Chinese Han population. The HD patients were classified into two groups: G1 and G2. G1 included 65 HD patients presented at least one AVF thrombosis episode and G2 included 80 HD patients without any episode of AVF thrombosis.
  
  
  RESULTS
  There were no significant differences in either HPA-3 a/b genotypes (aa, ab, and bb) frequency distribution (p = 0.396) or allele (a and b) frequency distribution (p = 0.146) between HD patients and control groups. However, there were significant differences in both HPA-3 a/b genotypes (aa, ab, and bb) distribution (χ(2) = 6.127, p = 0.047) and allele (a and b) frequency distribution (χ(2) = 5.954, p = 0.015) between G1 and G2. The relative risk of native AVF dysfunction in ab + bb patients compared with that of aa patients was 2.31 (95% confidence interval: 1.18-4.52).
  
  
  CONCLUSIONS
  These findings suggest an association between AVF thrombosis and the HPA-3b allele, and it is likely that HPA-3 a/b polymorphisms could be useful markers for potential risk of native AVF thrombosis in HD patients.
authors.affiliation: Department of Nephrology, Affiliated Hospital of Nantong University, Nantong, PR China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wu
authors.forename: Ding-Wu
authors.initials: DW
authors.lastname: Zhang
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Cheng
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Fan
mesh_terms: D000328:Adult; D001166:Arteriovenous Shunt, Surgical; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000235:genetics* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D040201:Platelet Membrane Glycoprotein IIb / Q000235:genetics*; D011110:Polymorphism, Genetic; D006435:Renal Dialysis / Q000009:adverse effects*; D012307:Risk Factors; D013927:Thrombosis / Q000209:etiology / Q000235:genetics*
chemical_list: D040201:Platelet Membrane Glycoprotein IIb