title: Construction and immunogenicity of recombinant swinepox virus expressing capsid protein of PCV2.
abstract: To explore development of a vaccine against PCV2 infections, the gene of capsid protein (Cap) was inserted into the swinepox virus (SPV) genome by homologous recombination. The recombinant swinepox virus expressing capsid protein (rSPV-cap) was verified by PCR, western blot and immunofluorescence assays. To evaluate the immunogenicity of rSPV-cap, twenty-four PCV2 seronegative minipigs were immunized with rSPV-cap, wild type SPV (wtSPV; negative control), or PBS (challenge control). After inoculation with PCV2, pigs in the rSPV-cap immunized group showed significantly higher average daily weight gain (ADG) and shorter fever duration compared with the wtSPV treated group (P<0.05). Cap-specific antibody in the rSPV-cap immunized group increased dramatically after vaccination and increased continuously over time. PCV2 genomic copies in the serum of rSPV-cap immunized pigs were significantly less compared with the wtSPV treated group at all time points after inoculation (P<0.01). Significant reduction in gross lung lesion scores, histopathological lung lesion scores, and lymph node lesion scores were noted in the rSPV-cap immunized group compared with the wtSPV treated group (P<0.01). The results suggested that the recombinant rSPV-cap provided pigs with significant protection from PCV2-associated disease; thus, it offers proof-of-principle for the development of a vaccine for the prevention of PCV2-associated disease in pigs.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hui-xing
authors.initials: HX
authors.lastname: Lin
authors.forename: Zhe
authors.initials: Z
authors.lastname: Ma
authors.forename: Hong-jie
authors.initials: HJ
authors.lastname: Fan
authors.forename: Cheng-ping
authors.initials: CP
authors.lastname: Lu
mesh_terms: D000818:Animals; D001835:Body Weight; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D018173:Circoviridae Infections / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D017925:Circovirus / Q000235:genetics / Q000276:immunology*; D004337:Drug Carriers; D005334:Fever / Q000517:prevention & control; D005822:Genetic Vectors; D006651:Histocytochemistry; D008168:Lung / Q000473:pathology; D008198:Lymph Nodes / Q000473:pathology; D018148:Suipoxvirus / Q000235:genetics*; D013552:Swine; D013553:Swine Diseases / Q000473:pathology / Q000517:prevention & control*; D013556:Swine, Miniature; D013997:Time Factors; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*
chemical_list: D036022:Capsid Proteins; D004337:Drug Carriers; D014614:Vaccines, Synthetic; D014765:Viral Vaccines