title: DNA interaction studies of a platinum (II) complex containing an antiviral drug, ribavirin: the effect of metal on DNA binding.
abstract: The water-soluble Pt (II) complex, [PtCl (DMSO)(N(4)N(7)-ribavirin)]· H(2)O (ribavirin is an antiviral drug) has been synthesized and characterized by physico-chemical and spectroscopic methods. The binding interactions of this complex with calf thymus DNA (CT-DNA) were investigated using fluorimetry, spectrophotometry, circular dichroism and viscosimetry. The complex binds to CT-DNA in an intercalative mode. The calculated binding constant, K(b), was 7.2×10(5) M(-1). In fluorimetric studies, the enthalpy (ΔH<0) and entropy (ΔS>0) changes of the reaction between the Pt (II) complex with CT-DNA showed hydrophobic interaction. In addition, CD study showed stabilization of the right-handed B form of CT-DNA. All these results prove that the complex interacts with CT-DNA via intercalative mode of binding. In comparison with the previous study of the DNA interaction with ribavirin, these results show that platinum complex has greater affinity to CT-DNA.
authors.affiliation: Department of Chemistry, Faculty of Science, Razi University, Kermanshah, Iran. nahidshahabadi@yahoo.com
authors.forename: Nahid
authors.initials: N
authors.lastname: Shahabadi
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Mirzaei kalar
authors.forename: Neda Hosseinpour
authors.initials: NH
authors.lastname: Moghadam
mesh_terms: D000042:Absorption; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004583:Electrons; D006151:Guanosine / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D010984:Platinum / Q000378:metabolism*; D012254:Ribavirin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014783:Viscosity
chemical_list: D000998:Antiviral Agents; D006151:Guanosine; D012254:Ribavirin; D010984:Platinum; D004247:DNA; C427203:calf thymus DNA