title: Mycelial fungi completely remediate di(2-ethylhexyl)phthalate, the hazardous plasticizer in PVC blood storage bag.
abstract: This pioneering work describes how simply, inexpensively and efficiently novel fungi utilize the alarming plasticizer, di(2-ethylhexyl)phthalate (DEHP) blended in PVC blood storage bags (BB). In order to quantify total DEHP (33.5%, w/w) present in BB, it was extracted using n-hexane and confirmed by GC-MS. Three mycelial fungi, viz., Aspergillus parasiticus, Fusarium subglutinans and Penicillium funiculosum isolated in our laboratory form heavily plastics-contaminated soil - either singly or in consortium - completely consumed intact DEHP physically bound to BB by static submerged growth (28 °C) in simple basal salt medium (BSM). A two-stage cultivation strategy was adopted for the complete removal of DEHP from BB in situ. During the first growth stage, almost 70% DEHP contained in the BB was consumed in 2 weeks, accompanied by increased fungal biomass (~0.15-0.35 g/g BB; OD ~7 at 600 nm) and a sharp declining (3.3) of initial pH (7.2). Spent BSM was replaced at this stagnant growth state (low pH), thus in the second stage, remaining DEHP bound to BB utilized completely (over 99%). Furthermore, A. parasiticus and F. subglutinans also grew well on scrapes of PVC water pipes in BSM. F. subglutinans was as efficient independently as consortium in completely utilizing the DEHP bound to BB, and these fungi offer great potentials for the inexpensive and eco-friendly bioremediation of phthalates in medical and allied PVC wastes on a large scale through a batch process in alleviating the plactics waste management issue.
authors.affiliation: Enzyme Technology Laboratory, Biotechnology Division, Department of Botany, University of Calicut, Kerala 673 635, India.
authors.forename: S
authors.initials: S
authors.lastname: Pradeep
authors.forename: Sailas
authors.initials: S
authors.lastname: Benjamin
mesh_terms: D001673:Biodegradation, Environmental; D001793:Blood Preservation / Q000295:instrumentation; D004271:DNA, Fungal / Q000235:genetics; D004051:Diethylhexyl Phthalate / Q000032:analysis / Q000378:metabolism*; D004785:Environmental Pollutants / Q000032:analysis / Q000378:metabolism*; D005658:Fungi / Q000502:physiology*; D010968:Plasticizers / Q000032:analysis / Q000378:metabolism*; D011143:Polyvinyl Chloride
chemical_list: D004271:DNA, Fungal; D004785:Environmental Pollutants; D010968:Plasticizers; D011143:Polyvinyl Chloride; D004051:Diethylhexyl Phthalate