title: Detection and serotyping of lyophilized nonculturable pneumococcal isolates.
abstract: One hundred fifty-two nonculturable lyophilized carriage pneumococcal isolates from a vaccine trial were subjected to PCR for serotyping, and 149 (98%) were successfully classified as vaccine or nonvaccine types, which were similar to viable isolates. The methodology will be useful for analysis of this and other studies where stored pneumococcal isolates fail to grow.
authors.affiliation: Department of Microbiology, Dhaka Shishu Hospital, Dhaka, Bangladesh.
authors.forename: Joyanta K
authors.initials: JK
authors.lastname: Modak
authors.forename: Mark C
authors.initials: MC
authors.lastname: Steinhoff
authors.forename: K
authors.initials: K
authors.lastname: Zaman
authors.forename: Maksuda
authors.initials: M
authors.lastname: Islam
authors.forename: Shams
authors.initials: S
authors.lastname: El Arifeen
authors.forename: Samir K
authors.initials: SK
authors.lastname: Saha
mesh_terms: D002353:Carrier State / Q000382:microbiology*; D005612:Freeze Drying; D005838:Genotype; D006801:Humans; D007223:Infant; D009035:Mothers; D011008:Pneumococcal Infections / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011309:Preservation, Biological / Q000379:methods*; D012703:Serotyping / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics*