title: A high-throughput approach for measuring temporal changes in the interactome.
abstract: Interactomes are often measured using affinity purification-mass spectrometry (AP-MS) or yeast two-hybrid approaches, but these methods do not provide stoichiometric or temporal information. We combine quantitative proteomics and size-exclusion chromatography to map 291 coeluting complexes. This method allows mapping of an interactome to the same depth and accuracy as AP-MS with less work and without overexpression or tagging. The use of triplex labeling enables monitoring of interactome rearrangements.
authors.affiliation: Department of Biochemistry & Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Anders R
authors.initials: AR
authors.lastname: Kristensen
authors.forename: Joerg
authors.initials: J
authors.lastname: Gsponer
authors.forename: Leonard J
authors.initials: LJ
authors.lastname: Foster
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism; D018929:Cell Culture Techniques; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D007553:Isotope Labeling; D025941:Protein Interaction Mapping / Q000379:methods*; D060066:Protein Interaction Maps; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013997:Time Factors
chemical_list: D000596:Amino Acids; D011506:Proteins