title: Discovery of a novel (+)-γ-lactamase from Bradyrhizobium japonicum USDA 6 by rational genome mining.
abstract: A novel (+)-γ-lactamase used for the resolution of racemic γ-lactam from Bradyrhizobium japonicum USDA 6 was found as a result of sequence-structure guided genome mining. It consists of 409 amino acids, only 49% of which are identical to the amino acid sequences of the known (+)-γ-lactamase from Sulfolobus solfataricus. This is only the third (+)-γ-lactamase gene to be reported.
authors.affiliation: State Key Laboratory of Chemical Resources Engineering, Beijing University of Chemical Technology, Beijing, People's Republic of China.
authors.forename: Shaozhou
authors.initials: S
authors.lastname: Zhu
authors.forename: Cuiyu
authors.initials: C
authors.lastname: Gong
authors.forename: Dawei
authors.initials: D
authors.lastname: Song
authors.forename: Shuaihua
authors.initials: S
authors.lastname: Gao
authors.forename: Guojun
authors.initials: G
authors.lastname: Zheng
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D000595:Amino Acid Sequence; D020369:Bradyrhizobium / Q000201:enzymology* / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D048229:Sulfolobus solfataricus / Q000235:genetics
chemical_list: D000581:Amidohydrolases; C401943:gamma-lactamase