title: Refolding of detergent-denatured lysozyme using β-cyclodextrin-assisted ion exchange chromatography.
abstract: Chromatography-based protein refolding is widely used. Detergent is increasingly used for protein solubilization from inclusion bodies. Therefore, it is necessary to develop a refolding method for detergent-denatured/solubilized proteins based on liquid chromatography. In the present work, sarkosyl-denatured/dithiothreitol-reduced lysozyme was used as a model, and a refolding method based on ion exchange chromatography, assisted by β-cyclodextrin, was developed for refolding detergent-denatured proteins. Many factors affecting the refolding, such as concentration of urea, concentration of β-cyclodextrin, pH and flow rate of mobile phases, were investigated to optimize the refolding conditions for sarkosyl-denatured lysozymes. The results showed that the sarkosyl-denatured lysozyme could be successfully refolded using β-cyclodextrin-assisted ion exchange chromatography.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, Xi'an, 710069, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Qinming
authors.initials: Q
authors.lastname: Zhang
authors.forename: Chaozhan
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D002645:Chickens; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003902:Detergents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism; D011489:Protein Denaturation; D058849:Protein Refolding; D012521:Sarcosine / Q000031:analogs & derivatives / Q000737:chemistry; D014508:Urea / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D003902:Detergents; D047392:beta-Cyclodextrins; C025231:sarkosyl; D014508:Urea; C421864:hen egg lysozyme; D009113:Muramidase; D012521:Sarcosine