title: Discrimination of Phellodendron amurense and P. chinense based on DNA analysis and the simultaneous analysis of alkaloids.
abstract: Phellodendri Cortex is the bark of the stems of Phellodendron amurense Ruprecht or P. chinense Schneider (Rutaceae), which is orginated from periderm. The internal transcribed spacer sequences of 20 originated plants and identified samples were analyzed. The result showed that the 99% of the base sequences of P. amurense were identical to that of P. chinense, but the differentiation of P. amurense and P. chinense was difficult. In addition, the ribulose-1, 5-bisphospate carboxylase large subunit (rbcL) intergenic spacer sequences of specific parts produced the same result. However, when the analysis was carried out by using the RAPD (randomly amplification polymorphism DNA) analysis method, which utilizes 48 randomly primers, it allowed us to confirm the polymorphism of P. amurense and P. chinense in 12 primers. A high-performance liquid chromatographic (HPLC) method was developed and validated for the simultaneous quantitation of berberine, palmatine and jatrorrhizine in a traditional herbal drug, Phellodendri Cortex. The HPLC method was applied successfully to the quantification of three constituents in the extract of twenty Phellodendri Cortex. The results indicated that the established HPLC and RAPD methods are suitable for the quantitative analysis and the quality control multi-simultaneous discrimination in Phellodendri Cortex.
authors.affiliation: Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
authors.forename: Jin Ah
authors.initials: JA
authors.lastname: Ryuk
authors.forename: Ming Shan
authors.initials: MS
authors.lastname: Zheng
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Lee
authors.forename: Chang Seob
authors.initials: CS
authors.lastname: Seo
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Seung Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Dong Cheul
authors.initials: DC
authors.lastname: Moon
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Lee
authors.forename: Je-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Ju Young
authors.initials: JY
authors.lastname: Park
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Byoung Seob
authors.initials: BS
authors.lastname: Ko
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D001483:Base Sequence; D001599:Berberine / Q000031:analogs & derivatives / Q000032:analysis; D001600:Berberine Alkaloids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D021901:DNA, Intergenic / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D057230:Limit of Detection; D008969:Molecular Sequence Data; D032102:Phellodendron / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D024301:Plant Bark; D010946:Plants, Medicinal; D011786:Quality Control; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics
chemical_list: D000470:Alkaloids; D001600:Berberine Alkaloids; D021901:DNA, Intergenic; D018744:DNA, Plant; D004365:Drugs, Chinese Herbal; C055785:jatrorrhizine; D001599:Berberine; D012273:Ribulose-Bisphosphate Carboxylase; C005413:palmatine