title: Microbiological applications of high-resolution melting analysis.
abstract: High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we describe HRM technology and provide examples of varied clinical microbiological applications to highlight the strengths and limitations of HRM analysis.
authors.affiliation: Menzies School of Health Research, Darwin, Northern Territory, Australia.
authors.forename: Steven Y C
authors.initials: SY
authors.lastname: Tong
authors.forename: Philip M
authors.initials: PM
authors.lastname: Giffard
mesh_terms: D003141:Communicable Diseases / Q000175:diagnosis*; D008828:Microbiological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D044366:Transition Temperature