title: Globosides but not isoglobosides can impact the development of invariant NKT cells and their interaction with dendritic cells.
abstract: Recognition of endogenous lipid Ag(s) on CD1d is required for the development of invariant NKT (iNKT) cells. Isoglobotrihexosylceramide (iGb3) has been implicated as this endogenous selecting ligand and recently suggested to control overstimulation and deletion of iNKT cells in α-galactosidase A-deficient (αGalA(-/-)) mice (human Fabry disease), which accumulate isoglobosides and globosides. However, the presence and function of iGb3 in murine thymus remained controversial. In this study, we generate a globotrihexosylceramide (Gb3)-synthase-deficient (Gb3S(-/-)) mouse and show that in thymi of αGalA(-/-)/Gb3S(-/-) double-knockout mice, which store isoglobosides but no globosides, minute amounts of iGb3 can be detected by HPLC. Furthermore, we demonstrate that iGb3 deficiency does not only fail to impact selection of iNKT cells, in terms of frequency and absolute numbers, but also does not alter the distribution of the TCR CDR 3 of iNKT cells. Analyzing multiple gene-targeted mouse strains, we demonstrate that globoside, rather than iGb3, storage is the major cause for reduced iNKT cell frequencies and defective Ag presentation in αGalA(-/-) mice. Finally, we show that correction of globoside storage in αGalA(-/-) mice by crossing them with Gb3S(-/-) normalizes iNKT cell frequencies and dendritic cell (DC) function. We conclude that, although detectable in murine thymus in αGalA(-/-)/Gb3S(-/-) mice, iGb3 does not influence either the development of iNKT cells or their interaction with peripheral DCs. Moreover, in αGalA(-/-) mice, it is the Gb3 storage that is responsible for the decreased iNKT cell numbers and impeded Ag presentation on DCs.
authors.affiliation: Department of Cellular and Molecular Pathology, German Cancer Research Center, 69120 Heidelberg, Germany. s.porubsky@dkfz.d
authors.forename: Stefan
authors.initials: S
authors.lastname: Porubsky
authors.forename: Anneliese O
authors.initials: AO
authors.lastname: Speak
authors.forename: Mariolina
authors.initials: M
authors.lastname: Salio
authors.forename: Richard
authors.initials: R
authors.lastname: Jennemann
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Bonrouhi
authors.forename: Rashad
authors.initials: R
authors.lastname: Zafarulla
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Singh
authors.forename: Julian
authors.initials: J
authors.lastname: Dyson
authors.forename: Bruno
authors.initials: B
authors.lastname: Luckow
authors.forename: Agnes
authors.initials: A
authors.lastname: Lehuen
authors.forename: Ernst
authors.initials: E
authors.lastname: Malle
authors.forename: Johannes
authors.initials: J
authors.lastname: Müthing
authors.forename: Frances M
authors.initials: FM
authors.lastname: Platt
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Cerundolo
authors.forename: Hermann-Josef
authors.initials: HJ
authors.lastname: Gröne
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002454:Cell Differentiation / Q000276:immunology*; D003713:Dendritic Cells / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D005915:Globosides / Q000172:deficiency / Q000502:physiology*; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D055611:Natural Killer T-Cells / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D014281:Trihexosylceramides / Q000172:deficiency / Q000502:physiology; D000519:alpha-Galactosidase / Q000235:genetics / Q000502:physiology
chemical_list: D005915:Globosides; D014281:Trihexosylceramides; C514781:isoglobotrihexosylceramide; D000519:alpha-Galactosidase