title: A lower limit of detection for atrazine was obtained using bioluminescent reporter bacteria via a lower incubation temperature.
abstract: The present article reports on the influence of various atrazine concentrations to the response of genetically modified Escherichia coli TV1061 bacterial cells while modulating the experimental conditions. Interesting increases of bioluminescence signals are recorded for E. coli TV1061 bacteria in the presence of 10 μg/mL atrazine concentration named "high-toxicity bacteria alert" when compared with 1 μg/mL -10 fg/mL atrazine termed "low-toxicity bacteria alert". Detecting the effect of atrazine via its effect on bioluminescence of bacteria has been carried out by two consecutive measurements (fresh and overnight modes) at different concentrations of analyte. We have shown that a more precise discrimination at lower-toxicity concentrations can be obtained through overnight incubation of bacteria with the analyte at 4 °C. In addition, centrifugation of bacterial cells and analyte dilutions has been performed in order to ensure a better interaction between the insoluble atrazine pesticide and the bacterial cells.
authors.affiliation: Laboratoire de Nanotechnologie et d'Instrumentation Optique, Institute Charles Delaunay, Université de Technologie de Troyes, UMR-STMR CNRS 6279, 12 rue Marie-Curie BP2060, 10010 Troyes Cedex, France.
authors.forename: Kun
authors.initials: K
authors.lastname: Jia
authors.forename: Evgeni
authors.initials: E
authors.lastname: Eltzov
authors.forename: Timothée
authors.initials: T
authors.lastname: Toury
authors.forename: Robert S
authors.initials: RS
authors.lastname: Marks
authors.forename: Rodica E
authors.initials: RE
authors.lastname: Ionescu
mesh_terms: D001280:Atrazine / Q000032:analysis* / Q000633:toxicity*; D003080:Cold Temperature; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000633:toxicity*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000592:standards*
chemical_list: D004785:Environmental Pollutants; D001280:Atrazine