title: miR-335 promotes cell proliferation by directly targeting Rb1 in meningiomas.
abstract: Meningiomas, one of the most common benign brain tumors in humans, arise from arachnoid cells in the brain meninges. Our investigations have revealed that miR-335 is a typical microRNA overexpressed in meningiomas in humans. Characterization of the effects of miR-335 overexpression in meningiomas demonstrated that elevated levels of miR-335 increased cell growth and inhibited cell cycle arrest in the G0/G1 phase in vitro; in addition, reduction of the miR-335 levels had the opposite effect on tumor growth and progression. Further, previous studies have shown that the mechanism of effect of miR-335 on the proliferation of meningioma cells is associated with alterations in the expression of human retinoblastoma 1 (Rb1). Our results indicate that miR-335 plays an essential role in the proliferation of meningioma cells by directly targeting the Rb1 signaling pathway. Thus, our results highlight a novel molecular interaction between miR-335 and Rb1, and miR-335 may represent a potential novel therapeutic agent to target the proliferation of meningioma cells.
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou, 215300, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Dongyi
authors.initials: D
authors.lastname: Jiang
authors.forename: Guan
authors.initials: G
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Wan
authors.forename: Shuguang
authors.initials: S
authors.lastname: Zhang
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zeng
authors.forename: Tianhong
authors.initials: T
authors.lastname: Pan
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western; D002453:Cell Cycle; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008579:Meningioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: C556347:MIRN335 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D016160:Retinoblastoma Protein; D008156:Luciferases