title: Diaminopropionic acid lipopeptides: characterization studies of polyplexes aimed at pDNA delivery.
abstract: Here we report a novel class of peptides-d-diaminopropionic acids (Dap)-for gene delivery. These peptides have attractive properties for gene delivery, and the advantage that they can be easily manipulated in relation to their composition, abiding with tailored-design. We characterized the toxicological and biophysical properties of DNA particles resulting from the interaction of the nucleic acid with a series of Dap(8) peptides conjugated to different alkyl groups. These peptides formed small and homogenous DNA particle populations that protected against DNase I degradation at non-toxic concentrations. However, despite the similarity between these peptides and others that are arginine-rich, and efficient vectors, functional studies suggest the need for additional modifications in the carriers to improve their DNA delivery efficiency. Taken together, these studies underscore the relevance of the overall structure of the carrier and the complexity of designing from scratch a carrier.
authors.affiliation: Department of Laboratory Medicine, Karolinska Institutet, Clinical Research Center, Karolinska University Hospital Huddinge, Hälsovägen 7, 141 83 Huddinge, Sweden. joana.viola@gmail.com
authors.forename: Joana R
authors.initials: JR
authors.lastname: Viola
authors.forename: Roger
authors.initials: R
authors.lastname: Strömberg
authors.forename: Oscar E
authors.initials: OE
authors.lastname: Simonson
authors.forename: Samir El
authors.initials: SE
authors.lastname: Andaloussi
authors.forename: C I Edvard
authors.initials: CI
authors.lastname: Smith
authors.forename: Merita
authors.initials: M
authors.lastname: Murtola
mesh_terms: D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D055666:Lipopeptides / Q000737:chemistry* / Q000633:toxicity; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D014162:Transfection; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000737:chemistry / Q000633:toxicity
chemical_list: D004337:Drug Carriers; D055666:Lipopeptides; D015091:beta-Alanine; C011757:2,3-diaminopropionic acid; D004247:DNA