title: [Breeding of transgenic mice expressing human tau isoform with P301L mutation and identification of homozygous transgenic mice].
abstract: OBJECTIVE
  To establish homozygous transgenic mouse strain expressing human tau isoform with P301L mutation.
  
  
  METHODS
  Five transgenic mice expressing human tau isoform with P301L mutation were obtained by microinjection into male nuclei. Homozygote and hemizygote were identified by PCR and real-time fluorescent quantitative PCR.
  
  
  RESULTS
  Ninety five homozygous transgenic mice were selected, and the results indicated that homozygous transgenic mice were superior to hemizygote in simulating the changes of biological characteristics.
  
  
  CONCLUSIONS
  Exogenous gene tau is able to stably transmit to next generation and the combination of SYBR Green real-time fluorescent quantitative PCR with the traditional mating is a fast, reliable and economical way to screen homozygous and hemizygous transgenic mice.
authors.affiliation: Department of Pharmacology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Wang
authors.forename: Ru-zhui
authors.initials: RZ
authors.lastname: Chen
authors.forename: Xiao-nani
authors.initials: XN
authors.lastname: Zhu
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Li
authors.forename: Xiu-juan
authors.initials: XJ
authors.lastname: Liu
authors.forename: Zhi-hui
authors.initials: ZH
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Na
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Liang
authors.forename: Guo-guang
authors.initials: GG
authors.lastname: Qiu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hai
authors.initials: H
authors.lastname: Wang
authors.forename: Xue-lan
authors.initials: XL
authors.lastname: Wang
mesh_terms: D000818:Animals; D005260:Female; D006720:Homozygote; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008845:Microinjections; D009154:Mutation; D016875:tau Proteins / Q000235:genetics*
chemical_list: D016875:tau Proteins