title: Multi-locus variable number of tandem repeat analysis for rapid and accurate typing of virulent multidrug resistant Escherichia coli clones.
abstract: One hundred E. coli isolates from Norway (n = 37), Sweden (n = 24), UK (n = 20) and Spain (n = 19), producing CTX-M-type - (n = 84), or SHV-12 (n = 4) extended spectrum β-lactamases, or the plasmid mediated AmpC, CMY-2 (n = 12), were typed using multi-locus sequence typing (MLST) and multi-locus variable number of tandem repeat analysis (MLVA). Isolates clustered into 33 Sequence Types (STs) and 14 Sequence Type Complexes (STCs), and 58 MLVA-Types (MTs) and 25 different MLVA-Type Complexes (MTCs). A strong agreement between the MLST profile and MLVA typing results was observed, in which all ST131-isolates (n = 39) and most of the STC-648 (n = 10), STC-38 (n = 9), STC-10 (n = 9), STC-405 (n = 8) and STC-23 (n = 6) isolates were clustered distinctly into MTC-29, -36, -20, -14, -10 and -39, respectively. MLVA is a rapid and accurate tool for genotyping isolates of globally disseminated virulent multidrug resistant E. coli lineages, including ST131.
authors.affiliation: Research Group for Host-Microbe Interactions, Department of Medical Biology, University of Tromsø, Tromsø, Norway. umaer.naseer@uit.no
authors.forename: Umaer
authors.initials: U
authors.lastname: Naseer
authors.forename: Barbro E
authors.initials: BE
authors.lastname: Olsson-Liljequist
authors.forename: Neil
authors.initials: N
authors.lastname: Woodford
authors.forename: Hiran
authors.initials: H
authors.lastname: Dhanji
authors.forename: Rafael
authors.initials: R
authors.lastname: Cantón
authors.forename: Arnfinn
authors.initials: A
authors.lastname: Sundsfjord
authors.forename: Bjørn-Arne
authors.initials: BA
authors.lastname: Lindstedt
mesh_terms: D015373:Bacterial Typing Techniques; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics; D018598:Minisatellite Repeats; D058885:Multilocus Sequence Typing; D009664:Norway; D013030:Spain; D013548:Sweden; D006113:United Kingdom; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; C477098:CTX-M-27, E coli; C103022:beta-lactamase CMY-2; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases