title: Beneficial effect of Lisosan G on cultured human microvascular endothelial cells exposed to oxidised low density lipoprotein.
abstract: OBJECTIVE
  Nutritional compounds which display anti-inflammatory and antioxidant effects have specific applications in preventing oxidative stress and endothelial dysfunction. In this study we evaluated the effect of Lisosan G (powder of Triticum sativum grains) on human microvascular endothelial cells (HMEC-1) exposed to oxidized low density lipoprotein (ox-LDL).
  
  
  METHODS
  The protective effects of Lisosan G were evaluated on human microvascular endothelial cells exposed to ox-LDL. Intercellular adhesion molecular-1 (ICAM-1), endothelin-1 (ET-1), and interleukin-6 (IL-6) concentrations and the expression of the respective genes were evaluated in response to incubation with ox-LDL, after co-incubation with ox-LDL and Lisosan G or exposed to Lisosan G alone. The analysis of LOX-1 gene was performed with RT-PCR semi quantitative method. The degree of oxidation induced in relation to control, was established by measurement of malondialdehyde (MDA) production.
  
  
  RESULTS
  The incubation with ox-LDL induced a significant increase in ICAM-1, IL-6 and ET-1 levels compared to the basal condition (P<0.01, P<0.05, and P<0.01, respectively), while in presence of Lisosan G, ICAM-1 levels showed a significant reduction both compared to the cultures treated with ox-LDL and control (P<0.01). IL-6 levels did not show any difference; ET-1 levels showed a partial reduction after co-treatment with Lisosan G, and also with Lisosan G alone, reduced the concentration below control (P<0.01). The modulation of these markers was confirmed by RT-PCR analysis. An association between MDA formation and the three markers production was observed. Semi-quantitative analysis of LOX-1 gene expression showed a significant up-regulation only after ox-LDL exposure.
  
  
  CONCLUSIONS
  The results demonstrate that Lisosan G may have an important role in the prevention of microcirculatory dysfunction.
authors.affiliation: Institute of Agricultural Biology & Biotechnology, CNR-Regione Toscana, Via Moruzzi 1, Pisa, Italy.
authors.forename: Valter
authors.initials: V
authors.lastname: Lubrano
authors.forename: Simona
authors.initials: S
authors.lastname: Baldi
authors.forename: Debora
authors.initials: D
authors.lastname: Napoli
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Longo
mesh_terms: D000704:Analysis of Variance; D015415:Biomarkers / Q000378:metabolism*; D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D019332:Endothelin-1 / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008833:Microcirculation / Q000187:drug effects* / Q000502:physiology; D055806:Microvessels / Q000166:cytology*; D010936:Plant Extracts / Q000494:pharmacology*; D028321:Plant Preparations; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051127:Scavenger Receptors, Class E / Q000378:metabolism
chemical_list: D015415:Biomarkers; D019332:Endothelin-1; D015850:Interleukin-6; D008077:Lipoproteins, LDL; C522933:Lisosan G; C495137:OLR1 protein, human; D010936:Plant Extracts; D028321:Plant Preparations; D051127:Scavenger Receptors, Class E; C099252:oxidized low density lipoprotein; D018799:Intercellular Adhesion Molecule-1