title: [Chemical constituents of Phymatopteris hastate and their antioxidant activity].
abstract: OBJECTIVE
  To study chemical constituents contained in Phymatopteris hastate and their antioxidant activity.
  
  
  METHODS
  Chemical constituents were separated and purified from P. hastate by using such methods as silica gel, Toyopearl HW-40C and HPLC preparative chromatography. Their structures were identified by spectroscopic methods such as NMR. Furthermore, 1, 1-diphenyl-2-picryl-hydrazyl(DPPH) method was used to assess the antioxidant activity of each compound.
  
  
  RESULTS
  Fourteen compounds were separated and identified as 4-O-beta-D-glucopyranosyl-ethyl-trans-caffeicate (1), kaempferlo-7-O-alpha-L-rhamnopyranside (2), kaempferol-3, 7-di-O-alpha-L-rhamnopyranoside (3), kaempferol-3-O-alpha-L-arabinofuranosyl-7-O-alpha-L-rhamnopyranoside (4), juglanin (5), naringin (6), naringenin-7-O-beta-D-glucopyranoside (7), trans-caffeic acid (8), trans-caffeic acid-3-O-beta-D-glucopyranoside (9), trans-cinnamic acid-4-O-beta-D- glucopyranoside (10), trans-melilotoside (11), cis-melilotoside (12), ethyl chlorogenate (13), protocatechuic acid (14). The antioxidation experiment showed an obvious antioxidant activity in compounds 1-9, 13-14.
  
  
  CONCLUSIONS
  All of the compounds were separated from this genus for the first time. Among them, compound 1 was not seen in literature reports and assumed to be a new artifact derived from compound 9 and ethanol. Compounds 1-9, 13-14 showed a remarkable antioxidant activity.
authors.affiliation: School of Parmaceutical Sciences, Basic Medical Research Center, Tianjin Medical University, Tianjin 300070, China. duanshilian@gmail.com
authors.forename: Shilian
authors.initials: S
authors.lastname: Duan
authors.forename: Sheng'an
authors.initials: S
authors.lastname: Tang
authors.forename: Nan
authors.initials: N
authors.lastname: Qin
authors.forename: Hongquan
authors.initials: H
authors.lastname: Duan
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000494:pharmacology; D044950:Flavanones / Q000032:analysis; D044949:Kaempferols / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D044949:Kaempferols; C005273:naringenin