title: [Oxidoreductase expression and protein storage in winter and spring cereals under extremal temperature condition].
abstract: Cultivation of wheat and barley plants under temperature stress fluctuations was the reason of reduction of acid protein common storage in the tissues more than 25% and the reason of reduction of peroxidase and superoxide dismutase common activities. There were no changes of common phenoloxidase activity. Activity redistribution between separate fractions of enzyme spectra were linked with enzyme expression changes. Alteration of peroxidase/oxidase ratio for separate fractions of electrophoretical spectra has been defined as a result of extreme temperature influence over a period of plant growing. At the same time there were no alterations in peroxidase/superoxide reductase fraction ratio in seedlings. The Vrn1-loci did not have a single meaning for expression of investigated oxidoreductases because their influence was determined by the "organism-environment" definite situation. In "normal" growth circumstances there was no correlation between the type of development and qualitative and quantitative characteristics of oxidoreductase spectra. The alteration of definite enzyme izoform degree in forming of plant stress response was different for spring and winter genotypes that testifies dependence of the functional state of the probed enzymes on allelic composition of locus Vrn1.
authors.forename: V A
authors.initials: VA
authors.lastname: Toptikov
authors.forename: L F
authors.initials: LF
authors.lastname: D'iachenko
authors.forename: V N
authors.initials: VN
authors.lastname: Totskiĭ
mesh_terms: D000483:Alleles; D002523:Edible Grain / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D056426:Genetic Loci; D005838:Genotype; D001467:Hordeum / Q000201:enzymology / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000235:genetics* / Q000378:metabolism; D010544:Peroxidases / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012621:Seasons; D013045:Species Specificity; D013482:Superoxide Dismutase / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D010544:Peroxidases; D014442:Monophenol Monooxygenase; D013482:Superoxide Dismutase