title: Low-level laser therapy vs. pulsed electromagnetic field on neonatal rat calvarial osteoblast-like cells.
abstract: To compare the effects of pulsed electromagnetic field (PEMF) and low-level laser therapy (LLLT) on osteoblast cells in a cell culture model. Fifty thousand neonatal rat calvarial osteoblast-like cells per milliliter were seeded and 0.06 mT PEMF, 0.2 mT PEMF, and LLLT at 808 nm were applied for 24 and 96 h on the cells. To evaluate cellular proliferation and differentiation, specimens were examined for DNA synthesis, alkaline phosphatase (ALP) activity, cell numbers, and viability of the cells. Morphological appearances of the cells were observed using scanning electron microcopy after 24 and 96 h of incubation. At 24 and 96 h, the control group had a higher cell proliferation than 0.06 and 0.2 mT PEMF groups (p=0.001). At 96 h, 0.2 mT PEMF group had higher cell proliferation rate than 0.06 mT PEMF and LLLT groups (p=0.001). The cell count and cell viability in 0.2 mT PEMF group were higher than the 0.06-mT PEMF and LLLT groups, although these differences were not statistically significant at 96 h (p>0.05). At 24 and 96 h, cell viability in the control group was higher than the test groups. Alkaline phosphatase levels of the groups were comparable in both time intervals (p>0.05). 0.2 mT PEMF application on osteoblast-like cells led to cell proliferation and differentiation better than 0.06 mT PEMF and LLLT at 808 nm, although a remarkable effect of both PEMF and LLLT could not be detected. The ALP activity of 0.2 and 0.06 mT PEMF and LLLT were comparable.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Emes
authors.forename: Kivanç
authors.initials: K
authors.lastname: Akça
authors.forename: Buket
authors.initials: B
authors.lastname: Aybar
authors.forename: Serhat
authors.initials: S
authors.lastname: Yalçın
authors.forename: Yeliz
authors.initials: Y
authors.lastname: Çavuşoğlu
authors.forename: Uğur
authors.initials: U
authors.lastname: Baysal
authors.forename: Halim
authors.initials: H
authors.lastname: Işsever
authors.forename: Belir
authors.initials: B
authors.lastname: Atalay
authors.forename: Pervin
authors.initials: P
authors.lastname: Vural
authors.forename: Mine
authors.initials: M
authors.lastname: Ergüven
authors.forename: Murat Cavit
authors.initials: MC
authors.lastname: Çehreli
authors.forename: Ayhan
authors.initials: A
authors.lastname: Bilir
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration / Q000528:radiation effects; D002454:Cell Differentiation / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D028022:Low-Level Light Therapy / Q000295:instrumentation; D055909:Magnetic Field Therapy / Q000295:instrumentation; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D004247:DNA; D000469:Alkaline Phosphatase