title: Focused DNA microarray analysis for sex-dependent gene expression of drug metabolizing enzymes, transporters and nuclear receptors in rat livers and kidneys.
abstract: Cytochrome P450(CYP)s are known to show a sexual dimorphic expression in rat  livers. However, the comprehensive analysis for the sex-dependent gene expressions of drug metabolizing enzymes except for CYPs, transporters and nuclear receptors in rat livers and kidneys has not been investigated yet. The purpose of the present study was to identify the novel drug metabolizing and pharmacokinetics (DMPK)-related gene(s) which show the sex difference in the mRNA expressions in rat livers and kidneys. Total RNAs were prepared from livers and kidneys in both male and female rats (Crl:CD(SD) and Crlj:WI). A DNA microarray analysis using a "GeneSQUARE Multiple Assay DNA Microarray Drug Metabolism Gene Expression for Rat" was performed. DMPK-related genes which showed sex differences in the mRNA expression were identified in rat livers or kidneys. Especially, the female dominant expressions of UDP glucuronosyltransferase (UGT) s were seen in rat livers and kidneys. The sex difference of UGT expressions in rats might be one of the causal factors of the sex difference of the biological response to UGT substrates.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Iwano
authors.forename: Eriko
authors.initials: E
authors.lastname: Higashi
authors.forename: Tomoya
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ando
authors.forename: Yohei
authors.initials: Y
authors.lastname: Miyamoto
mesh_terms: D000082:Acetaminophen / Q000493:pharmacokinetics* / Q000633:toxicity; D000818:Animals; D016000:Cluster Analysis; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008658:Inactivation, Metabolic; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010599:Pharmacokinetics; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics* / Q000378:metabolism; D012737:Sex Factors
chemical_list: D026901:Membrane Transport Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D000082:Acetaminophen; D012313:RNA; D003577:Cytochrome P-450 Enzyme System