title: Preparation, cellular internalization, and biocompatibility of highly fluorescent PMMA nanoparticles.
abstract: Methacrylate monomers were functionalized with a 4-hydroxythiazole chromophore and copolymerized with methyl methacrylate via RAFT. Nanoparticles of 120 and 500 nm in size were prepared without using stabilizers/surfactants. For comparative studies, preparative ultracentrifugation was applied for the separation into small and large particle fractions. All suspensions were characterized by DLS, AUC, and SEM and tested regarding their stability during centrifugation and re-suspension, autoclavation, and incubation in cell culture media. In vitro studies with mouse fibroblast cell line and differently sized NP showed a particle uptake into cells. Biocompatibility, non-toxicity, and hemocompatibility were demonstrated using a XTT assay, a live/dead staining, and an erythrocyte aggregation and hemolysis assay.
authors.affiliation: Laboratory of Organic and Macromolecular Chemistry, Friedrich-Schiller-University Jena, Humboldtstrasse 10, Jena, Germany.
authors.forename: Antje
authors.initials: A
authors.lastname: Vollrath
authors.forename: David
authors.initials: D
authors.lastname: Pretzel
authors.forename: Christian
authors.initials: C
authors.lastname: Pietsch
authors.forename: Igor
authors.initials: I
authors.lastname: Perevyazko
authors.forename: Stephanie
authors.initials: S
authors.lastname: Schubert
authors.forename: George M
authors.initials: GM
authors.lastname: Pavlov
authors.forename: Ulrich S
authors.initials: US
authors.lastname: Schubert
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D051379:Mice; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D010316:Particle Size; D058105:Polymerization; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D013844:Thiazoles; D019904:Polymethyl Methacrylate