title: Aluminum induces oxidative burst, cell wall NADH peroxidase activity, and DNA damage in root cells of Allium cepa L.
abstract: Plants under stress incur an oxidative burst that involves a rapid and transient overproduction of reactive oxygen species (ROS: O(2) (•-) , H(2) O(2) , (•) OH). We hypothesized that aluminum (Al), an established soil pollutant that causes plant stress, would induce an oxidative burst through the activation of cell wall-NADH peroxidase (NADH-PX) and/or plasma membrane-associated NADPH oxidase (NADPH-OX), leading to DNA damage in the root cells of Allium cepa L. Growing roots of A. cepa were treated with Al(3+) (800 μM of AlCl(3) ) for 3 or 6 hr without or with the pretreatment of inhibitors specific to NADH-PX and NADPH-OX for 2 hr. At the end of the treatment, the extent of ROS generation, cell death, and DNA damage were determined. The cell wall-bound protein (CWP) fractions extracted from the untreated control and the Al-treated roots under the aforementioned experimental conditions were also subjected to in vitro studies, which measured the extent of activation of peroxidase/oxidase, generation of (•) OH, and DNA damage. Overall, the present study demonstrates that the cell wall-bound NADH-PX contributes to the Al-induced oxidative burst through the generation of ROS that lead to cell death and DNA damage in the root cells of A. cepa. Furthermore, the in vitro studies revealed that the CWP fraction by itself caused DNA damage in the presence of NADH, supporting a role for NADH-PX in the stress response. Altogether, this study underscores the crucial function of the cell wall-bound NADH-PX in the oxidative burst-mediated cell death and DNA damage in plants under Al stress.
authors.affiliation: Department of Botany, Molecular Biology and Genomics Laboratory, Berhampur University, Berhampur, India.
authors.forename: V Mohan M
authors.initials: VM
authors.lastname: Achary
authors.forename: Narasimham L
authors.initials: NL
authors.lastname: Parinandi
authors.forename: Brahma B
authors.initials: BB
authors.lastname: Panda
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000704:Analysis of Variance; D016923:Cell Death / Q000187:drug effects; D002473:Cell Wall / Q000201:enzymology; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D019697:Onions; D010544:Peroxidases / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000187:drug effects*; D012989:Soil Pollutants / Q000633:toxicity*
chemical_list: D017382:Reactive Oxygen Species; D012989:Soil Pollutants; D000535:Aluminum; D010544:Peroxidases; C021845:NAD+ peroxidase