title: Regeneration of nucleus pulposus tissue in an ovine intervertebral disc degeneration model by cell-free resorbable polymer scaffolds.
abstract: Degeneration of intervertebral discs (IVDs) occurs frequently and is often associated with lower back pain. Recent treatment options are limited and treat the symptoms rather than regenerate the degenerated disc. Cell-free, freeze-dried resorbable polyglycolic acid (PGA)-hyaluronan implants were used in an ovine IVD degeneration model. The nucleus pulposus of the IVD was partially removed, endoscopically. PGA-hyaluronan implants were immersed in autologous sheep serum and implanted into the disc defect. Animals with nucleotomy only served as controls. The T2-weighted/fat suppression sequence signal intensity index of the operated discs, as assessed by magnetic resonance imaging (MRI), showed that implantation of the PGA-hyaluronan implant improved (p = 0.0066) the MRI signal compared to controls at 6 months after surgery. Histological analysis by haematoxylin and eosin and safranin O staining showed the ingrowth of cells with typical chondrocytic morphology, even cell distribution, and extracellular matrix rich in proteoglycan. Histomorphometric analyses confirmed that the implantation of the PGA-hyaluronan scaffolds improved (p = 0.027) the formation of regenerated tissue after nucleotomy. Disc heights remained stable in discs with nucleotomy only as well as after implantation of the implant. In conclusion, implantation of cell-free polymer-based implants after nucleotomy induces nucleus pulposus tissue regeneration and improves disc water content in the ovine model.
authors.affiliation: Tissue Engineering Laboratory, Campus Charité Mitte, Charité-Universitätsmedizin Berlin, Germany; Spine Centre Berlin, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Woiciechowsky
authors.forename: Alexander
authors.initials: A
authors.lastname: Abbushi
authors.forename: Maria L
authors.initials: ML
authors.lastname: Zenclussen
authors.forename: Pablo
authors.initials: P
authors.lastname: Casalis
authors.forename: Jan Philipp
authors.initials: JP
authors.lastname: Krüger
authors.forename: Undine
authors.initials: U
authors.lastname: Freymann
authors.forename: Michaela
authors.initials: M
authors.lastname: Endres
authors.forename: Christian
authors.initials: C
authors.lastname: Kaps
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D004195:Disease Models, Animal; D006820:Hyaluronic Acid / Q000494:pharmacology; D007403:Intervertebral Disc / Q000378:metabolism* / Q000473:pathology; D055959:Intervertebral Disc Degeneration / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D011100:Polyglycolic Acid / Q000494:pharmacology; D012038:Regeneration; D012756:Sheep; D054457:Tissue Scaffolds; D055675:Viscosupplements / Q000494:pharmacology
chemical_list: D055675:Viscosupplements; D011100:Polyglycolic Acid; D006820:Hyaluronic Acid