title: Photodynamic inactivation of prions by disulfonated hydroxyaluminium phthalocyanine.
abstract: Sulfonated phthalocyanines (Pcs) are cyclic tetrapyrroles that constitute a group of photosensitizers. In the presence of visible light and diatomic oxygen, Pcs produce singlet oxygen and other reactive oxygen species that have known degradation effects on lipids, proteins and/or nucleic acids. Pcs have been used successfully in the treatment of bacterial, yeast and fungal infections, but their use in the photodynamic inactivation of prions has never been reported. Here, we evaluated the photodynamic activity of the disodium salt of disulfonated hydroxyaluminium phthalocyanine (PcDS) against mouse-adapted scrapie RML prions in vitro. PcDS treatment of RML brain homogenate resulted in a time- and dose-dependent inactivation of prions. The photodynamic potential of Pcs offers a new way to inactivate prions using biodegradable compounds at room temperature and normal pressure, which could be useful for treating thermolabile materials and liquids.
authors.affiliation: Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Studnickova 7, 128 00 Prague 2, Czech Republic.
authors.forename: Olga
authors.initials: O
authors.lastname: Janouskova
authors.affiliation: Centre for Organic Chemistry Ltd, Rybitvi 296, 53354 Rybitvi, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Rakusan
authors.affiliation: Centre for Organic Chemistry Ltd, Rybitvi 296, 53354 Rybitvi, Czech Republic.
authors.forename: Marie
authors.initials: M
authors.lastname: Karaskova
authors.affiliation: Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague, Studnickova 7, 128 00 Prague 2, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Holada
mesh_terms: D000818:Animals; D001921:Brain; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*; D054833:Isoindoles; D051379:Mice; D015394:Molecular Structure; D009474:Neurons; D010778:Photochemotherapy / Q000379:methods*; D011312:Pressure; D011328:Prions / Q000187:drug effects*; D013451:Sulfonic Acids / Q000737:chemistry / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
chemical_list: D007211:Indoles; D054833:Isoindoles; D011328:Prions; D013451:Sulfonic Acids; C577121:disulfonated hydroxyaluminium phthalocyanine; C013647:phthalocyanine