title: Paucity of CD34-positive cells and increased expression of high-mobility group box 1 in coronary thrombus with type 2 diabetes mellitus.
abstract: To examine the presence of CD34-positive cells and intranuclear factors in acute coronary thrombi, we compared thrombi in patients with type 2 diabetes mellitus (DM, n = 21) and without DM (n = 29). Immunohistochemical staining revealed the constitutive presence of platelets, fibrin, erythrocytes, neutrophils, extracellular high-mobility group box 1 (HMGB-1), and histone H3 in all thrombi. There were significantly more oval or flat CD34-positive cells and significantly larger HMGB-1-positive areas in the thrombi from patients with DM. The flat CD34-positive cells expressed ecto-nucleoside triphosphate diphosphohydrolase (a platelet aggregation inhibitor). The number of CD34-positive cells was negatively correlated with the serum levels of glucose and hemoglobin A1c, whereas the HMGB-1-positive area was positively correlated with the levels of serum glucose. The paucity of CD34-positive cells and the high levels of HMGB-1 expression in acute coronary thrombi from patients with type 2 DM could facilitate thrombus formation.
authors.affiliation: Department of Pathology Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. atsushi@fc.miyazaki-u.ac.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamashita
authors.forename: Kensaku
authors.initials: K
authors.lastname: Nishihira
authors.forename: Yunosuke
authors.initials: Y
authors.lastname: Matsuura
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kawahara
authors.forename: Kinta
authors.initials: K
authors.lastname: Hatakeyama
authors.forename: Teruto
authors.initials: T
authors.lastname: Hashiguchi
authors.forename: Ikuro
authors.initials: I
authors.lastname: Maruyama
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagi
authors.forename: Masanori
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Fujimura
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kitamura
authors.forename: Yoshisato
authors.initials: Y
authors.lastname: Shibata
authors.forename: Yujiro
authors.initials: Y
authors.lastname: Asada
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D018952:Antigens, CD34 / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D016009:Chi-Square Distribution; D003328:Coronary Thrombosis / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D024243:HMGB1 Protein / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015854:Up-Regulation
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D024243:HMGB1 Protein; C517652:hemoglobin A1c protein, human