title: Iron supplementation reduces the erosive potential of a cola drink on enamel and dentin in situ.
abstract: Iron has been suggested to reduce the erosive potential of cola drinks in vitro.
  
  
  OBJECTIVE
  The aim of this study was to evaluate in situ the effect of ferrous sulfate supplementation on the inhibition of the erosion caused by a cola drink.
  
  
  METHODS
  Ten adult volunteers participated in a crossover protocol conducted in two phases of 5 days, separated by a washout period of 7 days. In each phase, they wore palatal devices containing two human enamel and two human dentin blocks. The volunteers immersed the devices for 5 min in 150 mL of cola drink (Coca-ColaTM, pH 2.6), containing ferrous sulfate (10 mmol/L) or not (control), 4 times per day. The effect of ferrous sulfate on the inhibition of erosion was evaluated by profilometry (wear). Data were analyzed by paired t tests (p<0.05).
  
  
  RESULTS
  The mean wear (±se) was significantly reduced in the presence of ferrous sulfate, both for enamel (control: 5.8±1.0 µm; ferrous sulfate: 2.8±0.6 µm) and dentin (control: 4.8±0.8 µm; ferrous sulfate: 1.7±0.7 µm).
  
  
  CONCLUSIONS
  The supplementation of cola drinks with ferrous sulfate can be a good alternative for the reduction of their erosive potential. Additional studies should be done to test if lower ferrous sulfate concentrations can also have a protective effect as well as the combination of ferrous sulfate with other ions.
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Melissa Thiemi
authors.initials: MT
authors.lastname: Kato
authors.forename: Marília Afonso Rabelo
authors.initials: MA
authors.lastname: Buzalaf
mesh_terms: D000328:Adult; D002253:Carbonated Beverages / Q000009:adverse effects*; D018592:Cross-Over Studies; D003743:Dental Enamel / Q000187:drug effects*; D003804:Dentin / Q000187:drug effects*; D005260:Female; D005296:Ferrous Compounds / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D018709:Statistics, Nonparametric; D013997:Time Factors; D014077:Tooth Erosion / Q000517:prevention & control*; D057085:Tooth Wear; D016896:Treatment Outcome
chemical_list: D005296:Ferrous Compounds; C020748:ferrous sulfate