title: Dehydration from conserved stem regions is fundamental for ligand-dependent conformational transition of the adenine-specific riboswitch.
abstract: Conformational dynamics and observed equilibrium constants for ligand binding of the adenine-specific riboswitch (add-A riboswitch) in the absence of Mg(2+) and presence of various concentrations of poly-ethylene glycol having an average molecular weight of 200 indicated that 54.2 water molecules were released from P2 and P3 stem regions of the add-A riboswitch during conformational transition upon the binding of 2-aminopurine, an analog of the natural ligand adenine.
authors.affiliation: Frontier Institute for Biomolecular Engineering Research, Konan University, 7-1-20 Minatojima-minamachi, Kobe 6500047, Japan.
authors.forename: Vinit
authors.initials: V
authors.lastname: Kumar
authors.forename: Tamaki
authors.initials: T
authors.lastname: Endoh
authors.forename: Kentaro
authors.initials: K
authors.lastname: Murakami
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
mesh_terms: D000225:Adenine / Q000737:chemistry*; D003890:Desiccation; D008024:Ligands; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D011092:Polyethylene Glycols / Q000737:chemistry; D058928:Riboswitch; D014867:Water / Q000737:chemistry
chemical_list: D008024:Ligands; D058928:Riboswitch; D014867:Water; D011092:Polyethylene Glycols; D000225:Adenine