title: Electron transfer dissociation mass spectrometry of hemoglobin on clinical samples.
abstract: A mass spectrometry-based assay combining the specificity of selected reaction monitoring and the protein ion activation capabilities of electron transfer dissociation was developed and employed for the rapid identification of hemoglobin variants from whole blood without previous proteolytic cleavage. The analysis was performed in a robust ion trap mass spectrometer operating at nominal mass accuracy and resolution. Subtle differences in globin sequences, resulting with mass shifts of about one Da, can be unambiguously identified. These results suggest that mass spectrometry analysis of entire proteins using electron transfer dissociation can be employed on clinical samples in a workflow compatible with diagnostic applications.
authors.affiliation: Biomedical Proteomics Research Group, Department of Human Protein Science, Faculty of Medicine, Geneva University, Switzerland.
authors.forename: Didia
authors.initials: D
authors.lastname: Coelho Graça
authors.forename: Pierre
authors.initials: P
authors.lastname: Lescuyer
authors.forename: Lorella
authors.initials: L
authors.lastname: Clerici
authors.forename: Yury O
authors.initials: YO
authors.lastname: Tsybin
authors.forename: Ralf
authors.initials: R
authors.lastname: Hartmer
authors.forename: Markus
authors.initials: M
authors.lastname: Meyer
authors.forename: Kaveh
authors.initials: K
authors.lastname: Samii
authors.forename: Denis F
authors.initials: DF
authors.lastname: Hochstrasser
authors.forename: Alexander
authors.initials: A
authors.lastname: Scherl
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006454:Hemoglobins / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D021122:Protein Subunits / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
chemical_list: D006454:Hemoglobins; D021122:Protein Subunits