title: The Microsporum canis genome is organized into five chromosomes based on evidence from electrophoretic karyotyping and chromosome end mapping.
abstract: The karyotype of Microsporum canis was analyzed by contoured-clamped homogeneous electric field (CHEF) gel electrophoresis. Four chromosomal bands that correspond to five chromosomes ranging from 3.0-6.2 Mb were identified, adding the total genome size to approximately 24.9 Mb. To confirm the number of chromosomes in M. canis, the number of telomeres was assessed by using a telomeric probe (TTAGGG)(4) in Southern blot analyses of digested genomic DNA. Treatment of M. canis DNA with Bal31 exonuclease revealed progressive shortening of the DNA fragments positive for the (TTAGGG)(4) sequence, supporting location of repeats at the chromosome ends. These results can aid in improving the understanding of the genetic characterization of M. canis and the molecular epidemiology of dermatophytoses caused by this fungus.
authors.affiliation: Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Luciene M
authors.initials: LM
authors.lastname: Coelho
authors.forename: Jeny R
authors.initials: JR
authors.lastname: Cursino-Santos
authors.forename: Gabriela F
authors.initials: GF
authors.lastname: Persinoti
authors.forename: Antonio
authors.initials: A
authors.lastname: Rossi
authors.forename: Nilce M
authors.initials: NM
authors.lastname: Martinez-Rossi
mesh_terms: D015139:Blotting, Southern; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal / Q000235:genetics*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004706:Endodeoxyribonucleases; D059646:Genome Size; D016681:Genome, Fungal / Q000235:genetics*; D007621:Karyotyping; D008864:Microsporum / Q000235:genetics*; D016615:Telomere
chemical_list: D004271:DNA, Fungal; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31