title: Enhancement of the tyrosinase inhibitory activity of Mori Cortex Radicis extract by biotransformation using Leuconostoc paramesenteroides PR.
abstract: Mori Cortex Radicis (MCR), the root bark of Morus alba L., consists of various phytochemicals and exhibits a strong inhibitory effect on tyrosinase. To enhance the tyrosinase inhibitory activity of MCR extract without further purification of bioactive compounds, whole MCR extract was biotransformed with crude enzyme extract from a selected lactic acid bacterium, Leuconostoc paramesenteroides PR (LP). Mulberroside A (MA), a major stilbene glucoside of MCR, contains two β-glucosyl residues at the C3 and C4' positions of oxyresveratrol (OXY). The crude enzyme of LP hydrolyzed the two glycosidic bonds of MA effectively, and 97.1% of MA was biotransformed into OXY within 2 h. Commercial almond β-glucosidase hydrolyzed only one site of the two glycosidic bonds of MA, and 68.7% of MA was biotransformed to OXY-glucoside. The tyrosinase inhibitory activity of the crude extract of MCR was increased approximately 6.5-fold by biotransformation using LP, and the IC(50) value of the transformed MCR was 3.7 µg/mL.
authors.affiliation: Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Gwanak-ro, Seoul 151-742, Korea.
authors.forename: Jung Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: You
authors.forename: Hye Yoon
authors.initials: HY
authors.lastname: Kang
authors.forename: Geun Eog
authors.initials: GE
authors.lastname: Ji
mesh_terms: D000363:Agaricales / Q000737:chemistry; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004187:Disaccharides / Q000378:metabolism*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007934:Leuconostoc / Q000737:chemistry / Q000201:enzymology*; D013058:Mass Spectrometry; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D031625:Morus / Q000737:chemistry* / Q000378:metabolism; D010936:Plant Extracts / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D012996:Solutions; D013267:Stilbenes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004187:Disaccharides; D005656:Fungal Proteins; D010936:Plant Extracts; D012996:Solutions; D013267:Stilbenes; C420606:mulberroside A; C034912:puag-haad; D014442:Monophenol Monooxygenase