title: Synthetic riboswitches for external regulation of genes transferred by replication-deficient and oncolytic adenoviruses.
abstract: Therapeutic gene transfer by replication-defective viral vectors or, for cancer treatment, by replication-competent oncolytic viruses shows high promise for treatment of major diseases. To ensure safety, timing or dosing in patients, external control of therapeutic gene expression is desirable or even required. In this study, we explored the potential of artificial aptazymes, ligand-dependent self-cleaving ribozymes, as an innovative tool for regulation of therapeutic gene expression. Importantly, aptazymes act on RNA intrinsically, independent of regulatory protein-nucleic acid interactions and stoichiometry, are non-immunogenic and of small size. These are key advantages compared with the widely used inducible promoters, which were also reported to lose regulation at high copy numbers, e.g. after replication of oncolytic viruses. We characterized aptazymes in therapeutic gene transfer utilizing adenovectors (AdVs), adeno-associated vectors (AAVs) and oncolytic adenoviruses (OAds), which are all in advanced clinical testing. Our results show similar aptazyme-mediated regulation of gene expression by plasmids, AdVs, AAVs and OAds. Insertion into the 5'-, 3'- or both untranslated regions of several transgenes resulted in ligand-responsive gene expression. Notably, aptazyme regulation was retained during OAd replication and spread. In conclusion, our study demonstrates the fidelity of aptazymes in viral vectors and oncolytic viruses and highlights the potency of riboswitches for medical applications.
authors.affiliation: Helmholtz-University Group Oncolytic Adenoviruses, Deutsches Krebsforschungszentrum (DKFZ, German Cancer Research Center) and Department of Dermatology, Heidelberg University Hospital, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Ketzer
authors.forename: Simon F
authors.initials: SF
authors.lastname: Haas
authors.forename: Sarah
authors.initials: S
authors.lastname: Engelhardt
authors.forename: Jörg S
authors.initials: JS
authors.lastname: Hartig
authors.forename: Dirk M
authors.initials: DM
authors.lastname: Nettelbeck
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D045744:Cell Line, Tumor; D003673:Defective Viruses / Q000235:genetics; D000229:Dependovirus / Q000235:genetics; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D050504:Oncolytic Viruses / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics* / Q000378:metabolism; D058928:Riboswitch; D014161:Transduction, Genetic; D019076:Transgenes; D020506:Untranslated Regions; D014779:Virus Replication
chemical_list: D016337:RNA, Catalytic; D058928:Riboswitch; D020506:Untranslated Regions