title: Quantitative analysis of cancer-associated gene methylation connected to risk factors in Korean colorectal cancer patients.
abstract: OBJECTIVE
  The purpose of this paper was to elucidate the potential methylation levels of adjacent normal and cancer tissues by comparing them with normal colorectal tissues, and to describe the correlations between the methylation and clinical parameters in Korean colorectal cancer (CRC) patients.
  
  
  METHODS
  Hypermethylation profiles of nine genes (RASSF1, APC, p16(INK4a), Twist1, E-cadherin, TIMP3, Smad4, COX2, and ABCB1) were examined with 100 sets of cancer tissues and 14 normal colorectal tissues. We determined the hypermethylation at a given level by a percent of methylation ratio value of 10 using quantitative methylation real-time polymerase chain reaction.
  
  
  RESULTS
  Nine genes' hypermethylation levels in Korean CRC patient tissues were increased more higher than normal colorectal tissues. However, the amounts of p16(INK4a) and E-cadherin gene hypermethylation in normal and CRC tissues were not significantly different nor did TIMP3 gene hypermethylation in adjacent normal and cancer tissues differ significantly. The hypermethylation of TIMP3, E-cadherin, ABCB1, and COX2 genes among other genes were abundantly found in normal colorectal tissues. The hypermethylation of nine genes' methylation in cancer tissues was not significantly associated with any clinical parameters. In Cohen's kappa test, it was moderately observed that RASSF1 was related with E-cadherin, and Smad4 with ABCB1 and COX2.
  
  
  CONCLUSIONS
  This study provides evidence for different hypermethylation patterns of cancer-associated genes in normal and CRC tissues, which may serve as useful information on CRC cancer progression.
authors.affiliation: Department of Preventive Medicine, Dong-A University College of Medicine, Busan, Korea.
authors.forename: Ho-Jin
authors.initials: HJ
authors.lastname: Kang
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Kim
authors.forename: Byoung-Gwon
authors.initials: BG
authors.lastname: Kim
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: You
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Lee
authors.forename: Dong-Il
authors.initials: DI
authors.lastname: Kim
authors.forename: Young-Seoub
authors.initials: YS
authors.lastname: Hong
mesh_terms: D015179:Colorectal Neoplasms / Q000235:genetics*; D019175:DNA Methylation; D018450:Disease Progression; D020869:Gene Expression Profiling; D006801:Humans; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction