Humanization by guided selections.
Guided selection provides a powerful tool for humanization of the preexisting nonhuman antibodies as exemplified by HUMIRA, the world's first human antibody approved. This chapter describes the sequential guided selection procedure in which mouse VL and VH domains are replaced sequentially with human VL and VH, respectively to derive completely human antibody. The detailed protocols for construction of phage-displayed antibody library, panning, screening, and characterization, are included to achieve successful selection of human antibody with similar characteristics to original mouse antibody.
Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Gwahangno, Yuseong-gu, Daejeon, Republic of Korea.
Sang Jick
SJ
Kim
Hyo Jeong
HJ
Hong
D000595:Amino Acid Sequence; D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000276:immunology*; D000915:Antibody Affinity / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D001435:Bacteriophages / Q000378:metabolism; D001667:Binding, Competitive; D003001:Cloning, Molecular; D018274:Electroporation; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D019151:Peptide Library; D016133:Polymerase Chain Reaction
D061067:Antibodies, Monoclonal, Humanized; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D019151:Peptide Library