DISCO synchrotron-radiation circular-dichroism endstation at SOLEIL.
The new synchrotron-radiation circular-dichroism (SRCD) endstation on the UV-visible synchrotron beamline DISCO has been commissioned at the SOLEIL synchrotron. The design has been focused on preservation of a high degree of linear polarization at high flux and moderate resolving power covering the vacuum ultraviolet to visible spectral range (125-600 nm). The beam dimensions have been set to 4 mm × 4 mm at 1 nm bandwidth for lower sample degradation. The nitrogen-purged sample chamber fits three types of sample holders accommodating conventional round cell mounting, automated rotation of the samples, as well as a microfluidic set-up. Automated temperature-controlled data collection on microvolumes is now available to the biology and chemistry communities. Macromolecules including membrane proteins, soluble proteins, bio-nanotubes, sugars, DNA and RNAs are now routinely investigated.
Synchrotron SOLEIL, L'Orme des Merisiers, 91192 Gif sur Yvette, France. refregiers@synchrotron-soleil.fr
Matthieu
M
Réfrégiers
Frank
F
Wien
Ha Phuong
HP
Ta
Lavanya
L
Premvardhan
Stéphane
S
Bac
Frederic
F
Jamme
Valerie
V
Rouam
Bruno
B
Lagarde
François
F
Polack
Jean Luc
JL
Giorgetta
Jean Paul
JP
Ricaud
Michel
M
Bordessoule
Alexandre
A
Giuliani
D002942:Circular Dichroism / Q000379:methods*; D046911:Macromolecular Substances / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry; D017356:Synchrotrons
D046911:Macromolecular Substances; D009211:Myoglobin