Design of a novel gut bacterial adhesion model for probiotic applications.
A new gut bacterial adhesion model has been developed. For this, a continuous-flow bioreactor packed with bacteria-coated beads was designed to simulate the gut lining and other features. In vitro model efficacy shows successful bacterial cell gut adhesions: bacterial adhesion was higher with mucin-alginate compared to controls. In feasibility study, adhesion of Lactobacillus fermentum NCIMB 5221 and Lactobacillus reuteri NCIMB 701359 was investigated for their metabolic activities for bile salt. Bile salt hydrolase (BSH)-active Lactobacillus reuteri exerted higher activity than non-BSH-active L. fermentum. This model has potential use in gut health, probiotic, bacterial cell gut adhesion and other delivery applications.
Biomedical Technology and Cell Therapy Research Laboratory, Faculty of Medicine, Department of Biomedical Engineering and Artificial Cells and Organs Research Centre, McGill University, Duff Medical Building, Montreal, Quebec, Canada.
Laetitia
L
Rodes
Michael
M
Coussa-Charley
Daniel
D
Marinescu
Arghya
A
Paul
Marc
M
Fakhoury
Sana
S
Abbasi
Afshan
A
Khan
Catherine
C
Tomaro-Duchesneau
Satya
S
Prakash
D000464:Alginates / Q000737:chemistry / Q000494:pharmacology; D000581:Amidohydrolases / Q000378:metabolism*; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism*; D019149:Bioreactors; D041981:Gastrointestinal Tract / Q000382:microbiology; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D052197:Limosilactobacillus fermentum / Q000187:drug effects / Q000254:growth & development*; D052200:Limosilactobacillus reuteri / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D008953:Models, Anatomic; D009077:Mucins / Q000737:chemistry / Q000494:pharmacology; D019936:Probiotics / Q000378:metabolism*; D013045:Species Specificity
D000464:Alginates; D001426:Bacterial Proteins; D001647:Bile Acids and Salts; D006603:Hexuronic Acids; D009077:Mucins; D020723:Glucuronic Acid; D000581:Amidohydrolases; C023154:choloylglycine hydrolase