Measuring antibody-antigen binding kinetics using surface plasmon resonance.
Surface plasmon resonance (SPR) is now widely embraced as a technology for monitoring a diverse range of protein-protein interactions and is considered almost de rigueur for characterizing antibody-antigen interactions. The technique obviates the need to label either of the interacting species and the binding event is visualized in real-time. Thus, it is ideally suited for screening crude, unpurified antibody samples that dominate early candidate panels following antibody selection campaigns. SPR returns both concentration and affinity data but when used correctly can also resolve the discrete component kinetic parameters (association and dissociation rate constants) of the affinity interaction. Herein, we outline some SPR-based generic antibody screening configurations and methodologies in the context of expediting data-rich ranking of candidate antibody panels and ensuring that antibodies with the optimal kinetic binding characteristics are reliably identified.
Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.
Stephen
S
Hearty
Paul
P
Leonard
Richard
R
O'Kennedy
D000937:Antigen-Antibody Reactions / Q000276:immunology*; D002021:Buffers; D055767:Immobilized Proteins / Q000378:metabolism; D007700:Kinetics; D012015:Reference Standards; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013696:Temperature
D002021:Buffers; D055767:Immobilized Proteins