Biomarkers of antioxidant status, inflammation, and cartilage metabolism are affected by acute intense exercise but not superoxide dismutase supplementation in horses.
Objectives were to evaluate effects of (1) repetitive arthrocentesis on biomarkers of inflammation (prostaglandin E(2), PGE(2)) and aggrecan synthesis (chondroitin sulfate-846; CS) in synovial fluid (SF); (2) exercise and superoxide dismutase (SOD) supplementation on biomarkers of inflammation, antioxidant status, and aggrecan synthesis, in horses. Preliminary trial. Standardbreds underwent four arthrocentesis procedures within 48 h and exhibited elevated CS and no changes in PGE(2). Exercise trial. this randomized crossover design used twelve Standardbred mares which received either treatment (3000 IU d(-1) oral SOD powder) or placebo (cellulose powder) for 6 wks which culminated with them running a repeated sprint exercise test (RSET). Samples were collected before (PRE), during (PEAK), and following exercise (POST). Exercise resulted in increased (P < 0.05) antioxidant defenses including erythrocyte SOD, total glutathione, glutathione peroxidase, gene transcripts for interferon-gamma, interleukin-10, and interleukin-1β in blood, and decreased plasma nitric oxide. Exercise increased (P < 0.05) SF CS and adjusted-PGE(2), and higher (P < 0.05) CS and PGE(2) were found in hock versus carpus joints. No treatment effects were detected. Results suggest normal adaptive responses likely due to exercise-induced tissue microdamage and oxidative stress. Additional research is needed to identify benefit(s) of SOD supplementation in horses.
Department of Animal Sciences, Rutgers the State University, New Brunswick, NJ 08901-8525, USA. emily lamprecht@cargill.com
Emily D
ED
Lamprecht
Carey A
CA
Williams
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D019587:Dietary Supplements; D006736:Horses / Q000097:blood*; D007249:Inflammation / Q000097:blood / Q000662:veterinary*; D007596:Joints / Q000378:metabolism / Q000473:pathology; D010805:Physical Conditioning, Animal; D013482:Superoxide Dismutase / Q000008:administration & dosage / Q000494:pharmacology*
D000975:Antioxidants; D015415:Biomarkers; D013482:Superoxide Dismutase