Mapping single-cell-substrate interactions by surface plasmon resonance microscopy.
We report the imaging of the cell-substrate adhesion of a single cell with subcellular spatial resolution. Osmotic pressure was introduced to provide a controllable mechanical stimulation to the cell attached to a substrate, and high-resolution surface plasmon resonance microscopy was used to map the response of the cell, from which local cell-substrate adhesion was determined. In addition to high spatial resolution, the approach is noninvasive and fast and allows for the continuous mapping of cell-substrate interactions and single-cell movements.
Center for Bioelectronics and Biosensors, Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
Wei
W
Wang
Shaopeng
S
Wang
Qiang
Q
Liu
Jie
J
Wu
Nongjian
N
Tao
D002448:Cell Adhesion; D002478:Cells, Cultured; D006046:Gold / Q000737:chemistry; D006801:Humans; D016023:Integrins / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
D016023:Integrins; D006046:Gold