Nucleoporins Nup160 and Seh1 are required for disease resistance in Arabidopsis.
Arabidopsis Nup160 and Seh1, encoding two predicted nucleoporins of the Nup107-160 nuclear pore sub-complex, were identified in a reverse genetics screen based on their requirement for basal disease resistance. Both genes also contribute to immunity conferred by Toll interleukin 1 receptor/nucleotide-binding/leucine-rich repeat (TNL)-type R proteins and constitutive resistance activated in the deregulated TNL mutant, snc1. Protein amounts of EDS1, a central regulator of TNL-triggered resistance, are reduced in seh1 and severely depleted in nup160 single mutants. Here, we investigate the impact of mutations in Nup160, Seh1 and a third complex member, MOS3/Nup96, on EDS1 protein accumulation in the snc1 auto-immune mutant background. In addition, we examine the subcellular localization of Seh1 in root tissues.
Department of Plant Cell Biology; Albrecht-von-Haller Institute for Plant Sciences, Göttingen, Germany.
Charlotte
C
Roth
Marcel
M
Wiermer
D017360:Arabidopsis / Q000166:cytology / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D060467:Disease Resistance / Q000276:immunology*; D009154:Mutation / Q000235:genetics; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000276:immunology*; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
D029681:Arabidopsis Proteins; D028861:Nuclear Pore Complex Proteins; C581069:Nup160 protein, Arabidopsis; C515667:Sec13 homolog 1, Arabidopsis