Hybridization and reaction-based fluorogenic nucleic acid probes.
We developed fluorogenic probes, which are photoactivated in the presence of specific nucleic acid templates with the release of fluorescent dyes. This templated reaction can be used to target specific nucleic acids in complex mixtures. We further demonstrate that this reaction can be monitored by single-molecule fluorescence imaging.
Anorganisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, INF 270, Heidelberg, Germany.
Subrata
S
Dutta
Benjamin
B
Flottmann
Mike
M
Heilemann
Andriy
A
Mokhir
D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D009696:Nucleic Acids / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*
D005456:Fluorescent Dyes; D009696:Nucleic Acids; D015345:Oligonucleotide Probes