Effects of schizandrin on the expression of thymic stromal lymphopoietin in human mast cell line HMC-1.
OBJECTIVE
Thymic stromal lymphopoietin (TSLP) plays an important role in allergic diseases such as asthma and atopic dermatitis. Schizandrin has various effects such as anti-asthmatic, anti-cancer and anti-inflammatory effects. However, the effect of schizandrin on the production of TSLP has not been clarified. Thus, we investigated how schizandrin inhibits the production of TSLP in the human mast cell line HMC-1 cells.


METHODS
We used enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, luciferase assay, and Western blot analysis to investigate the effects of schizandrin.


RESULTS
Schizandrin inhibited the production and mRNA expression of TSLP in HMC-1 cells. The maximal inhibition rate of TSLP production by schizandrin (10 μM) was 68.62 ± 3.47%. Schizandrin inhibited the translocation and luciferase activity of nuclear factor-κB induced by phorbol myristate acetate plus A23187. In the activated HMC-1 cells, the activation of caspase-1 was increased, whereas the activation of caspase-1 was decreased by pretreatment with schizandrin.


CONCLUSIONS
These results suggest that schizandrin can be used to treat inflammatory and atopic diseases through the inhibition of TSLP.
Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Phil-Dong
PD
Moon
Biochip Research Center, Hoseo University, 165, Sechul-ri, Baebang-myun, Asan, Chungnam 336-795, Republic of Korea.
Hyun-Ja
HJ
Jeong
Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea. Electronic address: hmkim@khu.ac.kr.
Hyung-Min
HM
Kim
D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D020170:Caspase 1 / Q000096:biosynthesis; D002460:Cell Line; D034242:Cyclooctanes / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D008156:Luciferases; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D011083:Polycyclic Compounds / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D000094632:Thymic Stromal Lymphopoietin
D034242:Cyclooctanes; D016207:Cytokines; D017705:Lignans; D011083:Polycyclic Compounds; D000001:Calcimycin; D008156:Luciferases; D020170:Caspase 1; C011105:schizandrin; D013755:Tetradecanoylphorbol Acetate; D000094632:Thymic Stromal Lymphopoietin