Post-transcriptional effects and interactions between chronic mild stress and acute sleep deprivation: regulation of translation factor and cytoplasmic polyadenylation element-binding protein phosphorylation.
Stress and restricted or disrupted sleep trigger adaptive responses in the brain at the level of gene transcription. We investigated the possible impact of chronic mild stress (CMS), acute sleep deprivation, and a combination of these in male rats on post-transcriptional mechanisms important for cognitive function and synaptic plasticity. Relationships between sleep architecture and translational regulators were also assessed. After four weeks of CMS, phosphorylation of two key translation factors, eukaryotic initiation factor 4E (eIF4E) and elongation factor 2 (eEF2), was enhanced in the prefrontal cortex, but unchanged in the hippocampus and dentate gyrus. Sleep deprivation decreased phosphorylated eIF4E in the dentate gyrus. In contrast, eEF2 phosphorylation was elevated in all brain regions after sleep deprivation. Thus, CMS and sleep deprivation, when given alone, have distinct region-specific effects. Furthermore, the combined treatment revealed striking interactions with eEF2 phosphorylation in which sleep deprivation counteracts the effect of CMS cortically and CMS modulates the effects of sleep deprivation in the hippocampus proper. Although CMS exposure alone had no effect in the hippocampus, it inhibited the sleep deprivation-induced eIF4E phosphorylation, while inducing phosphorylation of a major regulatory RNA-binding protein, cytoplasmic polyadenylation element-binding protein (CPEB) in the combined treatment. CMS had no effect on plasma corticosterone, but led to disruption of sleep. Sleep quality and sleep quantity in non-stressed animals showed predictive changes in eIF4E and eEF2 phosphorylation cortically. Prior exposure to CMS abolishes this relationship. We conclude that CMS and acute sleep deprivation have interactive and brain region-specific effects on translational regulators of relevance to mechanisms of stress responsiveness and sleep homeostasis.
Department of Biological and Medical Psychology, University of Bergen, Jonas Lies Vei 91, N-5009 Bergen, Norway. janne.gronli@uib.no
Janne
J
Grønli
Grethe
G
Dagestad
Anne Marita
AM
Milde
Robert
R
Murison
Clive R
CR
Bramham
D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000502:physiology; D001921:Brain / Q000378:metabolism*; D003345:Corticosterone / Q000097:blood; D004195:Disease Models, Animal; D004569:Electroencephalography; D004576:Electromyography; D039561:Eukaryotic Initiation Factor-4E; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D020652:Peptide Elongation Factor 2; D010766:Phosphorylation; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012892:Sleep Deprivation / Q000097:blood / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D013315:Stress, Psychological / Q000097:blood / Q000209:etiology / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D039561:Eukaryotic Initiation Factor-4E; D020652:Peptide Elongation Factor 2; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D003345:Corticosterone