Inhibition of HIV-1 protease expression in T cells owing to DNA aptamer-mediated specific delivery of siRNA.
Targeted delivery is a promising way to improve the safety and efficiency of siRNA delivery. We show that a DNA aptamer could be used to deliver siRNA into CD4(+) T cells specifically. The DNA aptamer was obtained from the conversion of a reported RNA aptamer that binds to CD4 protein on the surface of T cells. It was covalently conjugated to the sense strand of the siRNA targeting HIV-1 protease (HIV-PR). The resulting DNA aptamer-siRNA chimera could specifically enter into CD4(+) T cells and efficiently knock down the expression of exogenous HIV-PR gene. This study provides the first evidence that the DNA aptamer with intrinsic stability has a greater potential to be used for siRNA delivery.
Faculty of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-Machi, Nagasaki 852-8521, Japan.
Qinchang
Q
Zhu
Takayuki
T
Shibata
Tsutomu
T
Kabashima
Masaaki
M
Kai
D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D016503:Drug Delivery Systems; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000235:genetics; D016333:HIV Protease / Q000235:genetics / Q000378:metabolism*; D011480:Protease Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
D052157:Aptamers, Nucleotide; D011480:Protease Inhibitors; D034741:RNA, Small Interfering; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1