Dual RNA-seq of pathogen and host.
A comprehensive understanding of host-pathogen interactions requires a knowledge of the associated gene expression changes in both the pathogen and the host. Traditional, probe-dependent approaches using microarrays or reverse transcription PCR typically require the pathogen and host cells to be physically separated before gene expression analysis. However, the development of the probe-independent RNA sequencing (RNA-seq) approach has begun to revolutionize transcriptomics. Here, we assess the feasibility of taking transcriptomics one step further by performing 'dual RNA-seq', in which gene expression changes in both the pathogen and the host are analysed simultaneously.
Institute for Molecular Infection Biology, University of Würzburg, D-97080, Germany.
Alexander J
AJ
Westermann
Stanislaw A
SA
Gorski
Jörg
J
Vogel
D000818:Animals; D001424:Bacterial Infections / Q000473:pathology; D019295:Computational Biology / Q000379:methods; D004195:Disease Models, Animal; D020869:Gene Expression Profiling / Q000379:methods*; D054884:Host-Pathogen Interactions; D006801:Humans; D009181:Mycoses / Q000473:pathology; D010935:Plant Diseases / Q000382:microbiology; D011528:Protozoan Infections / Q000473:pathology; D017423:Sequence Analysis, RNA / Q000379:methods*