The hUPF1-NMD factor controls the cellular transcript levels of different genes of complex I of the respiratory chain.
In this study the impact of hUPF1 and hUPF2 knockdown on alternative splicing (AS) isoforms of different genes encoding subunits of respiratory chain complex I and complex IV is described. As expected, loss of both hUPF1 and hUPF2 led to impairment of nonsense-mediated mRNA decay (NMD) and accumulation of PTC-containing NMD substrates generated by both complex I and complex IV genes. The levels of some complex I splice variants, which did not contain PTC as well as the level of some complex I canonical transcripts were, however, affected only by hUPF1 knockdown. This finding confirms that NMD plays a role in the maintenance of the transcriptome integrity and reveals a specific impact of hUPF1 on the regulation of complex I genes.
Department of Basic Medical Sciences, Section of Medical Biochemistry, University of Bari Aldo Moro, Bari, Italy. damianopanelli@biochem.uniba.it
Damiano
D
Panelli
Francesca Paola
FP
Lorusso
Raffaella
R
Trentadue
Alessandro
A
Stella
Anna Maria
AM
Sardanelli
Sergio
S
Papa
D017398:Alternative Splicing; D018389:Codon, Nonsense; D042967:Electron Transport Complex I / Q000235:genetics* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D059365:Nonsense Mediated mRNA Decay; D020365:RNA Helicases; D034622:RNA Interference; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D059467:Transcriptome / Q000235:genetics*
D018389:Codon, Nonsense; D007527:Isoenzymes; D016601:RNA-Binding Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C468286:UPF2 protein, human; D003576:Electron Transport Complex IV; D020365:RNA Helicases; C101828:UPF1 protein, human; D042967:Electron Transport Complex I