Detection of IS6110 and HupB gene sequences of Mycobacterium tuberculosis and bovis in the aortic tissue of patients with Takayasu's arteritis.
BACKGROUND
Takayasu's arteritis (TA) is a chronic inflammatory disease affecting the large arteries and their branches; its etiology is still unknown. In individuals suffering from TA, arterial inflammation progresses to stenosis and/or occlusion, leading to organ damage and affecting survival. Relation of TA with Mycobacterium tuberculosis has been known, but there have been only a few systematic studies focusing on this association. The IS6110 sequence identifies the Mycobacterium tuberculosis complex and the HupB establishes the differences between M. tuberculosis and M. bovis. Our objective was to search the presence of IS6110 and HupB genes in aorta of patients with TA.


METHODS
We analyzed aorta tissues embedded in paraffin from 5760 autopsies obtained from our institution, we divided the selected samples as cases and controls;


METHODS
aortic tissues of individuals with Takayasu's arteritis. Control positive: aortic tissues (with tuberculosis disease confirmed) and control negative with other disease aortic (atherosclerosis).


RESULTS
Of 181 selected aorta tissues, 119 fulfilled the corresponding criteria for TA, TB or atherosclerosis. Thus 33 corresponded to TA, 33 to tuberculosis (TB) and 53 to atherosclerosis. The mean age was 22 ± 13, 41 ± 19, and 57 ± 10, respectively. IS6110 and HupB sequences were detected in 70% of TA tissues, 82% in tuberculosis, and in 32% with atherosclerosis. Important statistical differences between groups with TA, tuberculosis versus atherosclerosis (p = 0.004 and 0.0001, respectively) were found.


CONCLUSIONS
We identified a higher frequency of IS6110 and HupB genes in aortic tissues of TA patients. This data suggests that arterial damage could occur due to previous infection with M. tuberculosis.
Department of Physiology, National Institute of Cardiology Ignacio Chavez, Juan Badiano No 1, Colonia Sección XVI, 14080 México DF, Mexico.
María Elena
ME
Soto
Ma
M
Del Carmen Ávila-Casado
Claudia
C
Huesca-Gómez
Gilberto Vargas
GV
Alarcon
Vicente
V
Castrejon
Virgilia
V
Soto
Sergio
S
Hernandez
Nilda
N
Espinola-Zavaleta
Maite
M
Vallejo
Pedro A
PA
Reyes
Ricardo
R
Gamboa
D000293:Adolescent; D000328:Adult; D000368:Aged; D001011:Aorta / Q000378:metabolism* / Q000382:microbiology*; D001426:Bacterial Proteins / Q000378:metabolism*; D002648:Child; D005260:Female; D006657:Histones / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009163:Mycobacterium bovis / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D013625:Takayasu Arteritis / Q000378:metabolism* / Q000382:microbiology*; D055815:Young Adult
D001426:Bacterial Proteins; D006657:Histones; C510666:HupB protein, Mycobacterium tuberculosis