PBK/TOPK expression during TPA-induced HL-60 leukemic cell differentiation.
OBJECTIVE
This study concerns expression of PBK/TOPK during differentiation of HL-60 leukemic cells induced by tetradecanoyl phorbol acetate (TPA).


METHODS
Wright-Giemsa staining was performed to observe morphological changes in the HL-60 cells, and flow cytometry was used to assess the cell cycle and CD11b, CD14, CD13, and CD33 expression. PBK/TOPK levels were determined by Western blot analysis.


RESULTS
After treating HL60 cells with 5.1×10⁻⁹ mmol/L of TPA for three days, the number of nitroblue-tetrazolium-positive cells and CD11b, CD13, and CD14 expression increased, whereas the PBK/TOPK levels decreased.


CONCLUSIONS
TPA can inhibit proliferation and induce differentiation of HL60 cells of the granulocytic or monocytic lineage. PBK/TOPK expression was downregulated during this process, whereas the Pho-PBK/TOPK expression was increased.
Department of Hematology, The Affiliated Hospital of Yan'an University, Yan'an, China.
Yu-Hong
YH
Liu
Xue-Mei
XM
Gao
Fan-Mei
FM
Ge
Zhe
Z
Wang
Wen-Qing
WQ
Wang
Xiao-Yong
XY
Li
D000954:Antigens, Surface / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D002273:Carcinogens / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D002273:Carcinogens; D020929:Mitogen-Activated Protein Kinase Kinases; C409022:PDZ-binding kinase; D013755:Tetradecanoylphorbol Acetate