Molecular signatures of cardiac defects in Down syndrome lymphoblastoid cell lines suggest altered ciliome and Hedgehog pathways.
Forty percent of people with Down syndrome exhibit heart defects, most often an atrioventricular septal defect (AVSD) and less frequently a ventricular septal defect (VSD) or atrial septal defect (ASD). Lymphoblastoid cell lines (LCLs) were established from lymphocytes of individuals with trisomy 21, the chromosomal abnormality causing Down syndrome. Gene expression profiles generated from DNA microarrays of LCLs from individuals without heart defects (CHD(-); n = 22) were compared with those of LCLs from patients with cardiac malformations (CHD(+); n = 21). After quantile normalization, principal component analysis revealed that AVSD carriers could be distinguished from a combined group of ASD or VSD (ASD+VSD) carriers. From 9,758 expressed genes, we identified 889 and 1,016 genes differentially expressed between CHD(-) and AVSD and CHD(-) and ASD+VSD, respectively, with only 119 genes in common. A specific chromosomal enrichment was found in each group of affected genes. Among the differentially expressed genes, more than 65% are expressed in human or mouse fetal heart tissues (GEO dataset). Additional LCLs from new groups of AVSD and ASD+VSD patients were analyzed by quantitative PCR; observed expression ratios were similar to microarray results. Analysis of GO categories revealed enrichment of genes from pathways regulating clathrin-mediated endocytosis in patients with AVSD and of genes involved in semaphorin-plexin-driven cardiogenesis and the formation of cytoplasmic microtubules in patients with ASD-VSD. A pathway-oriented search revealed enrichment in the ciliome for both groups and a specific enrichment in Hedgehog and Jak-stat pathways among ASD+VSD patients. These genes or related pathways are therefore potentially involved in normal cardiogenesis as well as in cardiac malformations observed in individuals with trisomy 21.
Univ Paris Diderot, Sorbonne Paris Cité, Unité de Biologie Fonctionnelle et Adaptative, EAC4413 CNRS, Paris, France.
Clémentine
C
Ripoll
Isabelle
I
Rivals
Emilie
E
Ait Yahya-Graison
Luce
L
Dauphinot
Evelyne
E
Paly
Clothilde
C
Mircher
Aimé
A
Ravel
Yann
Y
Grattau
Henri
H
Bléhaut
André
A
Mégarbane
Guy
G
Dembour
Bénédicte
B
de Fréminville
Renaud
R
Touraine
Nicole
N
Créau
Marie Claude
MC
Potier
Jean Maurice
JM
Delabar
D000818:Animals; D002460:Cell Line; D002877:Chromosomes, Human / Q000235:genetics; D004314:Down Syndrome / Q000150:complications* / Q000473:pathology*; D006343:Heart Septal Defects / Q000150:complications* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006345:Heart Septal Defects, Ventricular / Q000150:complications* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000473:pathology*; D051379:Mice; D010641:Phenotype; D015398:Signal Transduction; D059467:Transcriptome; D055815:Young Adult
D053823:Hedgehog Proteins