Platelet-dependent thrombography gives a distinct pattern of in vitro thrombin generation after surgery with cardio-pulmonary bypass: potential implications.
BACKGROUND
Bleeding remains a potentially lethal complication of cardio-pulmonary bypass (CPB) surgery. The purpose of this study was to obtain a better insight into in vitro thrombin generation in the context of CPB.


METHODS
We used Calibrated Automated Thrombography to assess blood coagulation of 10 low-risk patients operated for valve replacement with CPB, under 2 experimental conditions, one implicating platelets as platelet dysfunction has been described to occur during CPB.


RESULTS
Our main finding was that CPB-induced coagulopathy was differently appreciated depending on the presence or absence of platelets: the decrease in thrombin generation was much less pronounced in their presence (mean endogenous thrombin potential change values before and after CPB were -3.9% in the presence of platelets and -39.6% in their absence).


CONCLUSIONS
Our results show that experimental conditions have a profound effect in the study of in vitro thrombin generation in the context of CPB.
Laboratoire d'Hématologie et Institut Cardiovasculaire Nancy, Centre Hospitalier Universitaire, Nancy, France.|Inserm U961, Nancy Université, Nancy, France.
Rose
R
Said
Laboratoire d'Hématologie et Institut Cardiovasculaire Nancy, Centre Hospitalier Universitaire, Nancy, France.|Inserm U961, Nancy Université, Nancy, France.
Véronique
V
Regnault
Laboratoire d'Hématologie et Institut Cardiovasculaire Nancy, Centre Hospitalier Universitaire, Nancy, France.|EFS Lorraine Champagne, Nancy, France.
Marie
M
Hacquard
Laboratoire d'Hématologie et Institut Cardiovasculaire Nancy, Centre Hospitalier Universitaire, Nancy, France.
Jean-Pierre
JP
Carteaux
Laboratoire d'Hématologie et Institut Cardiovasculaire Nancy, Centre Hospitalier Universitaire, Nancy, France.|Inserm U961, Nancy Université, Nancy, France.|EFS Lorraine Champagne, Nancy, France.|Haematology Laboratory, CHU Nancy, Rue du Morvan, 54511, Vandoeuvre les Nancy Cedex, France.
Thomas
T
Lecompte