Rapid detection of the recently emerged Bordetella pertussis strains with the ptxP3 pertussis toxin promoter allele by real-time PCR.
Bordetella pertussis strains with the pertussis toxin promoter allele ptxP3 have expanded and replaced resident ptxP1 strains in several European countries. We developed an allele-specific real-time PCR method to identify strains with the allele ptxP3, and investigated the emergence of ptxP3 strains by genotyping Finnish clinical isolates (n = 524) from 1953 to 2010. The first ptxP3 strain was detected in 1994, and has become predominant since 2003. Our results demonstrate that the allele-specific real-time PCR is a suitable method for rapid detection of ptxP3 strains, and show the emergence and establishment of ptxP3 strains in Finland.
Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland.
T
T
Kallonen
J
J
Mertsola
F R
FR
Mooi
Q
Q
He
D000483:Alleles; D001886:Bordetella pertussis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D005387:Finland / Q000453:epidemiology; D005798:Genes, Bacterial; D006801:Humans; D015994:Incidence; D058889:Molecular Typing / Q000379:methods*; D037342:Pertussis Toxin / Q000235:genetics*; D015995:Prevalence; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014917:Whooping Cough / Q000453:epidemiology / Q000382:microbiology
D004269:DNA, Bacterial; D037342:Pertussis Toxin