Highly sensitive electrochemiluminescent nanobiosensor for the detection of palytoxin.
Marine toxins appear to be increasing in many areas of the world. An emerging problem in the Mediterranean Sea is represented by palytoxin (PlTX), one of the most potent marine toxins, frequently detected in seafood. Due to the high potential for human toxicity of PlTX, there is a strong and urgent need for sensitive methods toward its detection and quantification. We have developed an ultrasensitive electrochemiluminescence-based sensor for the detection of PlTX, taking advantage of the specificity provided by anti-PlTX antibodies, the good conductive properties of carbon nanotubes, and the excellent sensitivity achieved by a luminescence-based transducer. The sensor was able to produce a concentration-dependent light signal, allowing PlTX quantification in mussels, with a limit of quantification (LOQ = 2.2 μg/kg of mussel meat) more than 2 orders of magnitude more sensitive than that of the commonly used detection techniques, such as LC-MS/MS.
Center of Excellence on Nanostructured Materials, Department of Chemical and Pharmaceutical Sciences, and INSTM, Unit of Trieste, University of Trieste, P.le Europa 1, 34127 Trieste, Italy.
Valeria Anna
VA
Zamolo
Giovanni
G
Valenti
Enrica
E
Venturelli
Olivier
O
Chaloin
Massimo
M
Marcaccio
Sabrina
S
Boscolo
Valentina
V
Castagnola
Silvio
S
Sosa
Federico
F
Berti
Giampaolo
G
Fontanive
Mark
M
Poli
Aurelia
A
Tubaro
Alberto
A
Bianco
Francesco
F
Paolucci
Maurizio
M
Prato
D000178:Acrylamides / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003064:Cnidarian Venoms; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005506:Food Contamination / Q000032:analysis*; D008163:Luminescent Measurements / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D000095702:Polyether Toxins
D000178:Acrylamides; D003064:Cnidarian Venoms; C010272:palytoxin; D000095702:Polyether Toxins