Role of platelet-derived growth factor in cerebral vasospasm after subarachnoid hemorrhage in rats.
OBJECTIVE
The role of platelet-derived growth factor (PDGF) remains unknown in cerebral vasospasm after subarachnoid hemorrhage (SAH). In this study, we examined the effects of PDGF receptor (PDGFR) inactivation on cerebral vasospasm in the endovascular perforation model of SAH in rats.


METHODS
Rats were assigned to sham, SAH plus vehicle, and SAH plus imatinib mesylate (imatinib) groups (n = 4 per group). Imatinib (50 mg/kg body weight), an inhibitor of the tyrosine kinases of PDGFR, or vehicle was administered intraperitoneally 30 min post-SAH. Vasospasm was evaluated in the left (perforation-sided) internal carotid artery by means of neurobehavioral tests, India ink angiography, and immunohistochemistry at 24 h after SAH.


RESULTS
Imatinib significantly inhibited post-SAH PDGFR activation in the left internal carotid artery, in which vasospasm was significantly prevented. Animal's neurobehavior also showed a tendency to improve by imatinib treatment.


CONCLUSIONS
PDGF may play an important role in the pathogenesis of vasospasm after SAH.
Department of Neurosurgery, Mie University Graduate School of Medicine, Mie, Japan. mshiba@clin.medic.mie-u.ac.jp
Masato
M
Shiba
Hidenori
H
Suzuki
Masashi
M
Fujimoto
Naoshi
N
Shimojo
Kyoko
K
Imanaka-Yoshida
Toshimichi
T
Yoshida
Kenji
K
Kanamaru
Satoshi
S
Matsushima
Waro
W
Taki
D000818:Animals; D001549:Benzamides; D002244:Carbon; D002343:Carotid Artery, Internal / Q000187:drug effects / Q000378:metabolism; D017023:Coronary Angiography; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D004791:Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D000068877:Imatinib Mesylate; D008297:Male; D009460:Neurologic Examination; D010879:Piperazines / Q000494:pharmacology / Q000627:therapeutic use; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D011743:Pyrimidines / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism; D013345:Subarachnoid Hemorrhage / Q000150:complications*; D020301:Vasospasm, Intracranial / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control
D001549:Benzamides; D004791:Enzyme Inhibitors; D010879:Piperazines; D010982:Platelet-Derived Growth Factor; D011743:Pyrimidines; C028433:chinese ink; D002244:Carbon; D000068877:Imatinib Mesylate; D020797:Receptor, Platelet-Derived Growth Factor beta