Extracellular hydrogen peroxide, produced through a respiratory burst oxidase/superoxide dismutase pathway, directs ingrowth wall formation in epidermal transfer cells of Vicia faba cotyledons.
The intricate, and often polarized, ingrowth walls of transfer cells (TCs) amplify their plasma membrane surface areas to confer a transport function of supporting high rates of nutrient exchange across apo-/symplasmic interfaces. The TC ingrowth wall comprises a uniform wall layer on which wall ingrowths are deposited. Signals and signal cascades inducing trans-differentiation events leading to formation of TC ingrowth walls are poorly understood. Vicia faba cotyledons offer a robust experimental model to examine TC induction as, when placed into culture, their adaxial epidermal cells rapidly (h) and synchronously form polarized ingrowth walls accessible for experimental observations. Using this model, we recently reported findings consistent with extracellular hydrogen peroxide, produced through a respiratory burst oxidase homolog/superoxide dismutase pathway, initiating cell wall biosynthetic activity and providing directional information guiding deposition of the polarized uniform wall. Our conclusions rested on observations derived from pharmacological manipulations of hydrogen peroxide production and correlative gene expression data sets. A series of additional studies were undertaken, the results of which verify that extracellular hydrogen peroxide contributes to regulating ingrowth wall formation and is generated by a respiratory burst oxidase homolog/superoxide dismutase pathway.
School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW Australia.
Xue
X
Xia
Hui-Ming
HM
Zhang
Felicity A
FA
Andriunas
Christina E
CE
Offler
John W
JW
Patrick
D001692:Biological Transport; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000502:physiology; D054338:Cell Transdifferentiation; D002473:Cell Wall / Q000378:metabolism / Q000502:physiology*; D018548:Cotyledon / Q000502:physiology; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008954:Models, Biological; D010088:Oxidoreductases / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism / Q000502:physiology*; D019441:Plant Epidermis / Q000254:growth & development / Q000378:metabolism*; D016897:Respiratory Burst; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000378:metabolism*; D031307:Vicia faba / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*
D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D013482:Superoxide Dismutase