Detection of Bcl-2 family member Bcl-G in mouse tissues using new monoclonal antibodies.
Bcl-G is an evolutionarily conserved member of the Bcl-2 family of proteins that has been implicated in regulating apoptosis and cancer. We have generated monoclonal antibodies that specifically recognise mouse Bcl-G and have used these reagents to analyse its tissue distribution and subcellular localisation using western blotting, immunohistochemistry and immunofluorescence. We found that Bcl-G predominantly resides in the cytoplasm and is present in a wide range of mouse tissues, including the spleen, thymus, lung, intestine and testis. Immunohistochemical analyses revealed that Bcl-G is expressed highly in mature spermatids in the testis, CD8(+) conventional dendritic cells (DCs) in hematopoietic tissues and diverse epithelial cell types, including those lining the gastrointestinal and respiratory tracts. The Bcl-G monoclonal antibodies represent new tools for studying this protein, using a variety of techniques, including immunoprecipitation and flow cytometry.
The Walter and Eliza Hall Institute of Medical Research, Molecular Genetics of Cancer Division, Melbourne, Australia.
M
M
Giam
J D
JD
Mintern
G J P
GJ
Rautureau
M G
MG
Hinds
A
A
Strasser
P
P
Bouillet
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D003593:Cytoplasm / Q000378:metabolism; D003713:Dendritic Cells / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011485:Protein Binding; D020033:Protein Isoforms / Q000032:analysis / Q000276:immunology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis* / Q000276:immunology; D013087:Spermatids / Q000378:metabolism
D000911:Antibodies, Monoclonal; C576156:BCL2L14 protein, mouse; D020033:Protein Isoforms; D019253:Proto-Oncogene Proteins c-bcl-2