The effect of stimuli on basophil-mediated atopic responses during asthmatic lying-in women and in newborns.
Morbidity from allergic diseases is increasing. Basophils play a critical role in systemic anaphylaxis and chronic allergic inflammation. The prenatal environment must be regarded as a possible early risk factor for allergic diseases in children. Our objective was to determine if basophils harvested from neonates genetically predisposed to atopic disease had different levels of CD63 expression and IL-4 release properties in response to various stimuli (peptidoglycan, Dermatophagoides farinae, hyperosmotic mannitol). Blood samples were collected from 16 asthmatic and 18 healthy women and their newborns. Peripheral blood basophil histamine was measured using the human basophil degranulation test (HBDT), whereas activation was assessed by flow cytometric measurement of CD63 expression on the cord blood basophil surface. IL-4 levels were quantified by ELISA following allergen stimulation. The basophil degranulation index (DI) in granulocytes harvested from the peripheral blood of asthmatic women was assessed following stimulation with peptidoglycan (PGN), Dermatophagoides farinae (Df ) extract, or hyperosmotic mannitol. The DI was significantly higher in atopic women than in healthy controls. Upregulation of CD63 on the cord blood basophil surface was also detected in response to these stimuli. Basophils purified from the cord blood of neonates born to atopic mothers produced more IL-4 compared to basophils purified from the controls. These data suggested that various stimuli play a role in augmenting allergic reactions via modulation of activated basophil cytokine secretion. It may require new methods to stabilize the basophils in allergic diseases.
Geriatrics Department, Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, Shanghai, China.
Ling
L
Yang
Yin-Shi
YS
Guo
Jin-Qi
JQ
Jiang
Xue-Jun
XJ
Guo
Yi-Ping
YP
Xu
Ye
Y
Tian
Ying
Y
Xiong
Li
L
Han
D000328:Adult; D039741:Antigens, Dermatophagoides / Q000276:immunology; D001249:Asthma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015549:Basophil Degranulation Test; D001491:Basophils / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006636:Histamine Release; D006801:Humans; D007231:Infant, Newborn; D015847:Interleukin-4 / Q000378:metabolism; D008353:Mannitol / Q000276:immunology; D010457:Peptidoglycan / Q000276:immunology; D060149:Tetraspanin 30 / Q000378:metabolism
D039741:Antigens, Dermatophagoides; C554586:CD63 protein, human; C508598:IL4 protein, human; D010457:Peptidoglycan; D060149:Tetraspanin 30; D015847:Interleukin-4; D008353:Mannitol