Ovarian-protective effects of clotrimazole on ovarian ischemia/reperfusion injury in a rat ovarian-torsion model.
OBJECTIVE
To evaluate the ovarian-protective effects of clotrimazole on ovarian ischemia/reperfusion injury in a rat ovarian-torsion model.


METHODS
32 Sprague-Dawley rats were randomly divided into four groups: (1) ischemia group (n = 8) in which only left adnexal torsion was performed for 2 h, but no treatment was given; (2) vehicle group (n = 8) in which left adnexal torsion was performed for 2 h and at the end of 2 h ischemia polyethylene glycol (3% PEG, 1 ml, i.p.) was administered and a 24-hour reperfusion was continued; (3) clotrimazole group (n = 8) in which left adnexal torsion was performed for 2 h and at the end of 2 h ischemia clotrimazole (30 mg/kg, i.p.) was administered and a 24-hour reperfusion was continued, and (4) control group (sham-operated, n = 6) in which no adnexal torsion and no treatment were given. The criteria for ovarian ischemia were follicular cell degeneration, vascular congestion, hemorrhage and infiltration by inflammatory cells. Each specimen was scored for each criterion (0, none; 1, mild; 2, moderate; 3, severe).


RESULTS
Clotrimazole significantly decreased plasma levels of serum malondialdehyde, ischemia-modified albumin, and total oxidant status.


CONCLUSIONS
This study showed the ovarian-protective effects of clotrimazole on ovarian ischemia/reperfusion injury.
Faculty of Medicine, Department of Obstetrics and Gynecology, Karadeniz Technical University, Trabzon, Turkey. drmaosmanaga @ gmail.com
Mehmet A
MA
Osmanağaoğlu
Murat
M
Kesim
Esin
E
Yuluğ
Ahmet
A
Menteşe
S Caner
SC
Karahan
D058888:14-alpha Demethylase Inhibitors / Q000627:therapeutic use*; D000818:Animals; D003022:Clotrimazole / Q000627:therapeutic use*; D005260:Female; D010049:Ovarian Diseases / Q000150:complications* / Q000473:pathology; D010053:Ovary / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology* / Q000517:prevention & control*; D014102:Torsion Abnormality / Q000150:complications* / Q000473:pathology
D058888:14-alpha Demethylase Inhibitors; D003022:Clotrimazole