Anti-diabetic effect of a traditional Chinese medicine formula.
An anti-diabetic TCM formula consisting of Schizandra chinensis Baill. (SC), Coptis chinensis (CC), Psidium guajava L. leaves (PG) and Morus alba L. leaves (MA) was developed based on its α-glucosidase, DPP-4 and AGE inhibitory activities in vitro using response surface methodology (RSM). Then, the in vivo study was carried out to confirm the anti-diabetic function of the formula. RSM results showed that the optimum anti-diabetic TCM formula is the combination SC (3000 μg mL(-1)), CC (80 μg mL(-1)), PG (374.56 μg mL(-1)) and MA (480 μg mL(-1)). For the in vivo study, insulin resistant mice were induced by high-fat/high-sucrose (HF/HS) feeding for 6 weeks. Administration of the developed formula significantly decreased non-fasting blood glucose in the HF/HS diet mice. Moreover, the formula decreased blood glucose levels in the insulin tolerance test. These results indicated that the anti-diabetic mechanism of the formula might be due to decreased insulin resistance. The serum fructosamine level in the high dose group was significantly lower than the HF/HS and normal control groups, indicating that the formula could improve middle term glucose levels and reduce the risk of complications. The contents of berberine and 1-deoxynojirimycin in the formula were 4.7 ± 0.4 and 77.1 ± 1.1 μg mL(-1), respectively. These two compounds can be used as indicators for quality control during production.
Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
Hsiu-Ju
HJ
Wang
Been-Huang
BH
Chiang
D000818:Animals; D039323:Coptis / Q000737:chemistry; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D018819:Dipeptidyl Peptidase 4; D054873:Dipeptidyl-Peptidase IV Inhibitors; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005951:Glucose Tolerance Test; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000008:administration & dosage*; D007328:Insulin; D007333:Insulin Resistance; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D031625:Morus / Q000737:chemistry; D008517:Phytotherapy; D018515:Plant Leaves / Q000737:chemistry; D031647:Psidium / Q000737:chemistry; D032141:Schisandra / Q000737:chemistry
D054873:Dipeptidyl-Peptidase IV Inhibitors; D004365:Drugs, Chinese Herbal; D017127:Glycation End Products, Advanced; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D007328:Insulin; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4