Polyunsaturated fatty acids inhibit mouse hepatic glucocorticoid receptor activation in vitro.
The effect of saturated fatty acids (SFAs) stearic and palmitic acids and polyunsaturated fatty acids (PUFAs) oleic, linoleic and arachidonic acids was studied on in vitro heat activation of mouse hepatic glucocorticoid receptor (GR) complex, as assessed by binding to DNA-cellulose and purified nuclei. Significant dose-dependent inhibition of heat activation of hormone-receptor complex by the PUFAs was observed. Linoleic and arachidonic acids were found to be more potent (caused approximately 70% inhibition maximally at 160 microM) inhibitors of GR heat activation, compared to oleic acid (approximately 38% inhibition at 40 microM). However, stearic and palmitic acids were unable to modulate GR heat activation, suggesting that the unsaturated moieties in PUFAs are possibly the important determinants of receptor activation. Thus, our study shows an inhibitory effect of PUFAs on in vitro hepatic GR activation.
Department of Biochemistry, North Eastern Hill University, Shillong 793 022, India. hsranhotra1@rediffmail.com
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D000818:Animals; D016718:Arachidonic Acid / Q000737:chemistry; D002467:Cell Nucleus / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005938:Glucocorticoids / Q000737:chemistry; D006358:Hot Temperature; D066298:In Vitro Techniques; D019787:Linoleic Acid / Q000737:chemistry; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019301:Oleic Acid / Q000737:chemistry; D019308:Palmitic Acid / Q000737:chemistry; D011485:Protein Binding; D011965:Receptors, Glucocorticoid / Q000378:metabolism*; D013229:Stearic Acids / Q000737:chemistry; D013696:Temperature
D005231:Fatty Acids, Unsaturated; D005938:Glucocorticoids; D011965:Receptors, Glucocorticoid; D013229:Stearic Acids; D016718:Arachidonic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; C031183:stearic acid; D002482:Cellulose; D004247:DNA; D019787:Linoleic Acid