Study of the genetic variability of porcine circovirus type 2 detected in Serbia and Slovenia.
Recent variants of porcine circovirus type 2 (PCV2) were obtained from tissues of domestic pigs with porcine circovirus associated disease and from randomly selected wild boar samples from Serbia and Slovenia. A 450-base-pair nucleotide sequence was obtained by PCR from the ORF2. The derived nucleotide and amino acid sequences were aligned and compared to the corresponding region of closely related PCV2 sequences determined in previous years and retrieved from the GenBank. The 30 Serbian and 17 Slovenian PCV2 sequences clustered into three previously determined genotypes (PCV2a: 7), (PCV2b: 38) and (PCV2d: 2). Three major variable regions, concerning 29 amino acid position substitutions within the ORF2, were observed, which further supports the segregation of the detected strains into three separate genotypes. This study indicates that PCV2b is the predominant genotype in Serbia and Slovenia and the detected PCV2 strains are closely related to those previously described in Europe and in other parts of the world.
University of Ljubljana Veterinary Faculty, National Veterinary Institute Gerbičeva 60 Ljubljana Slovenia.
Ivan
I
Toplak
Sava
S
Lazić
Diana
D
Lupulović
Jasna
J
Prodanov-Radulović
Zsolt
Z
Becskei
Radoslav
R
Došen
Tamaš
T
Petrović
D000818:Animals; D017925:Circovirus; D005838:Genotype; D010802:Phylogeny; D055771:Serbia; D017524:Slovenia