Manipulating expression of tonoplast transporters.
Plant vacuoles have multifaceted roles including turgor maintenance, cytosolic pH and ionic homeostasis, plant protection against environmental stress, detoxification, pigmentation, and cellular signaling. These roles are achieved through the coordinated activities of many proteins in the tonoplast (vacuolar membrane), of which the proton pumps and ion transporters have been modified for improved abiotic stress tolerance in transgenic plants. Here we describe a method to manipulate vacuolar H(+)-pyrophosphatase in turfgrass and evaluate the impact of the modified tonoplast on the phenotype, biochemistry, and physiology of the transgenics. Creeping bentgrass (Agrostis stolonifera L.) plants overexpressing an Arabidopsis vacuolar H(+)-pyrophosphatase AVP1 exhibited improved growth and enhanced salt tolerance, likely associated with increased photosynthesis, relative water content, proline production, and Na(+) uptake. These transgenic plants also had decreased solute leakage in the leaf tissues and increased concentrations of Na(+), K(+), Cl(-), and total phosphorus in the root tissues. Similar strategies can be employed to manipulate other tonoplast transporters and in other plant species to produce transgenic plants with improved performance under various abiotic stresses.
Department of Genetics and Biochemistry, Clemson University, Clemson, SC, USA.
Zhigang
Z
Li
Man
M
Zhou
Qian
Q
Hu
Shane
S
Reighard
Shuangrong
S
Yuan
Ning
N
Yuan
Bekir
B
San
Dayong
D
Li
Haiyan
H
Jia
Hong
H
Luo
D031703:Agrostis / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D023061:Gene Order; D043564:Inorganic Pyrophosphatase / Q000235:genetics*; D018515:Plant Leaves / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D010957:Plasmids / Q000235:genetics; D055049:Salt Tolerance / Q000235:genetics; D055051:Salt-Tolerant Plants / Q000235:genetics*; D013312:Stress, Physiological; D014170:Transformation, Genetic
D029681:Arabidopsis Proteins; C505048:AVP1 protein, Arabidopsis; D043564:Inorganic Pyrophosphatase