[Optimizing expression of the capsid protein VP2 from human Bocavirus and establish it's seroepidemiology assying methord].
OBJECTIVE
To obtain sufficient recombinant VP2 protein of human Bocavirus and establish it's seroepidemiology assying metbord. METHORD: Tbe capsid protein VP2 DNA genes of HBoV1 and 2 were optimized in accordance with tbe usage of the favorite codons in K coil so as to enhance its protein expression in prokaryotic expressing system. The protein was purified by Ni-NTA column, and its antigenicity was determined by Western Blot. Then establish ELISA to detect the specific anti-VP2 IgG antibodies against HBoV1 and 2 in healthy children aged 3-6 years in Nanjing, China.


RESULTS
The recombinant protein 6 x His-VP2 was produced in a larger quantity at 25 degrees C induced by IPTG (1 mmol/L) over night and purified by Ni-NTA column. Seropositive rates of HBoV1 and 2 were 62.2% and 55.5% and their mixed seropositivity was 37%.


CONCLUSIONS
The optimizing expression of the capsid protein VP2 from human Bocavirus constructed successfully and get a high yield under certain conditions. The established ELISA could be used to further analyze seroepidemiology of HBoV in China.
Wenzhou Medical College, Zhejiang 317500, China.
Ye-Xia
YX
Hao
Ji-Min
JM
Gao
Yu
Y
Jin
Xiao-Le
XL
Li
Jing-Song
JS
Li
Zhi-Ping
ZP
Xie
Yuan-Yun
YY
Ao
Xi-Qian
XQ
Chen
Ke-Na
KN
Chen
Zhao-Jun
ZJ
Duan
D036022:Capsid Proteins / Q000032:analysis / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D056661:Human bocavirus / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D016036:Seroepidemiologic Studies
D036022:Capsid Proteins