Production of antibody fragments using the baculovirus-insect cell system.
The baculovirus-insect cell system is effective in the production of large quantities of biologically active recombinant proteins. The secretory production of an antibody Fab fragment in lepidopteran insect cells infected with a recombinant baculovirus that contains both the heavy-chain (Hc; Fd fragment) and light-chain (Lc) genes of the Fab fragment is described in this chapter. The recombinant baculovirus can be generated rapidly and efficiently through site-specific transposon-mediated insertion of foreign genes into a baculovirus genome called a bacmid in Escherichia coli. Factors influencing the Fab fragment production are discussed.
Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan.
Takanori
T
Furuta
Takafumi
T
Ogawa
Hideki
H
Yamaji
D000818:Animals; D016367:Baculoviridae / Q000378:metabolism*; D004274:DNA, Recombinant / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis* / Q000302:isolation & purification; D008967:Molecular Biology / Q000379:methods*; D061987:Sf9 Cells; D018411:Spodoptera / Q000166:cytology; D013194:Staining and Labeling
D004274:DNA, Recombinant; D007140:Immunoglobulin Fab Fragments