Large-scale quantitative glycoproteomics analysis of site-specific glycosylation occupancy.
Disease-associated aberrant glycosylation may be protein specific and glycosylation site specific. Quantitative assessment of glycosylation changes at a site-specific molecular level may represent one of the initial steps for systematically revealing the glycosylation abnormalities associated with a disease or biological state. Comparative quantitative profiling of glycoproteome to provide accurate quantification of site-specific glycosylation occupancy has been a challenging task, requiring a concerted approach drawing from a variety of techniques. In this report, we present a quantitative glycoproteomics method that allows global scale identification and comparative quantification of glycosylation site occupancy using mass spectrometry. We further demonstrated this approach by quantitatively characterizing the N-glycoproteome of human pancreas.
Department of Medicine, University of Washington, Seattle, WA 98195, USA. shengp@medicine.washington.edu
Sheng
S
Pan
Yasuko
Y
Tamura
Ru
R
Chen
Damon
D
May
Martin W
MW
McIntosh
Teresa A
TA
Brentnall
D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry; D010179:Pancreas / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
D006023:Glycoproteins