Grape seed proanthocyanidin extract alleviates ouabain-induced vascular             remodeling through regulation of endothelial function.
Recent studies indicate that chronic ouabain treatment leads to hypertension             and hypertensive vascular remodeling. Grape seed proanthocyanidin extract (GSPE)             has been reported to be effective in treating arteriosclerosis, while little is             known about its effect on systolic blood pressure and vascular remodeling. In             this study, the effects of GSPE on systolic blood pressure and vascular remodeling             were analyzed by treating ouabain-induced hypertensive rats with GSPE (250 mg/kg·d).             The expression of nitric oxide (NO) and endothelin-1 (ET-1) in thoracic aorta             was examined by ELISA; the mRNA and protein levels of TGF-β1 were detected using             real-time PCR and western blotting, respectively. The results showed that the             systolic blood pressure was significantly decreased following treatment with GSPE,             with blocked vascular remodeling. The ET-1 content was reduced while NO production             was increased in the GSPE group, which showed improved vascular endothelial function.             Moreover, GSPE also reduced TGF-β1 expression in the thoracic aorta, which is             a determinant in vascular remodeling. In conclusion, GSPE antagonized ouabain-induced             hypertension and vascular remodeling and is recommended as a potential anti-hypertensive             agent for patients with hypertensive vascular diseases.
Department of Geriatrics, Shandong University Qilu Hospital, Jinan, Shandong 250012, PR China.
Xiangju
X
Liu
Jie
J
Qiu
Shaohua
S
Zhao
Beian
B
You
Xiang
X
Ji
Yan
Y
Wang
Xiaopei
X
Cui
Qian
Q
Wang
Haiqing
H
Gao
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001794:Blood Pressure / Q000187:drug effects; D019332:Endothelin-1 / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000502:physiology; D056604:Grape Seed Extract / Q000494:pharmacology*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000737:chemistry; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*
D019332:Endothelin-1; D056604:Grape Seed Extract; C511402:Grape Seed Proanthocyanidins; D044945:Proanthocyanidins; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D009569:Nitric Oxide; D010042:Ouabain