Antibacterial, anti-chlamydial, and cytotoxic activities of a marine snail (Hexaplex trunculus) phospholipase A2: an in vitro study.
In order to report pharmacological characterization of marine snail (Hexaplex trunculus) hepatopancreatic phospholipase A(2) (mSDPLA(2)), we have talked for the first time the antimicrobial activity against different pathogenic bacterial strains, anti-chlamydial activity as well as its cytotoxic activity against McCoy cell lines. mSDPLA(2), showed a high level of activity towards Gram-positive bacteria as Staphylococcus aureus and Staphylococcus epidermidis. Whereas Gram-negative bacteria, unfortunately, exhibited a higher resistance, mSDPLA(2) was also found to have a strong cytotoxic activity, causing significant morphological alterations of the McCoy cell lines surfaces and to be a hinder to the proliferation. Moreover, mSDPLA(2) proved to have a very potent anti-chlamydial activity. Over 95 % inhibition of chlamydial inclusions were obtained at a concentration of 10 μg/ml of mSDPLA(2) after 24 h postinfection. Interestingly, at a concentration of 10 μg/ml of mSDPLA(2), the proliferation of McCoy cells was not affected. Approximately 50 % inhibition of cell growth was obtained with a concentration of 37 μg/mL of mSDPLA(2). mSDPLA(2) could be considered as an excellent candidate for the development of a new anti-infective agent. This enzyme showed significant antimicrobial activities.
Laboratoire de biochimie et de génie enzymatique des lipases, Université de Sfax-Tunisie, ENIS route de Soukra, BPW, 1173 Sfax, Tunisia.
Zied
Z
Zarai
Houda
H
Gharsallah
Adnane
A
Hammami
Hafedh
H
Mejdoub
Sofiane
S
Bezzine
Youssef-Talel
YT
Gargouri
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000633:toxicity*; D059001:Aquatic Organisms / Q000201:enzymology*; D002460:Cell Line; D002689:Chlamydia / Q000187:drug effects*; D051379:Mice; D054467:Phospholipases A2 / Q000494:pharmacology* / Q000633:toxicity*; D012908:Snails / Q000201:enzymology*
D000900:Anti-Bacterial Agents; D054467:Phospholipases A2