Snake venomics and antivenomics of Protobothrops mucrosquamatus and Viridovipera stejnegeri from Taiwan: keys to understand the variable immune response in horses.
The proteomes of the venoms of the snakes Viridovipera stejnegeri and Protobothrops mucrosquamatus from Taiwan were characterized by N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of in-gel generated tryptic peptides. Proteins belonging to the following toxin classes were identified: metalloproteinase, phospholipase A(2) (PLA(2)), serine proteinase, C-type lectin-like, CRISP, l-amino acid oxidase, disintegrin, and peptides (vasoactive and inhibitors of SVMPs). Nine horses were immunized with a mixture of these venoms. All horses developed a satisfactory immune response against lethality of the venom of V. stejnegeri, whereas only three horses reached the accepted neutralizing potency against the venom of P. mucrosquamatus. Antivenoms were prepared from pools of 'good responder' (GR) and 'poor responder' (PR) horses and compared by antivenomics and neutralization tests. A similar neutralizing response was observed between the GR and PR antivenoms against the venom of V. stejnegeri, whereas antivenom from PR had a lower neutralizing activity against effects of P. mucrosquamatus venom than antivenom from GR. The low potency of the plasma of some horses against this venom is a consequence of the low immunogenicity of the neurotoxic PLA(2) trimucrotoxin. Our results provide clues for innovating the immunization scheme to generate improved antivenoms.
Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.
Mauren
M
Villalta
Davinia
D
Pla
Su Lin
SL
Yang
Libia
L
Sanz
Alvaro
A
Segura
Mariángela
M
Vargas
Pei Yu
PY
Chen
María
M
Herrera
Ricardo
R
Estrada
Ya Fen
YF
Cheng
Cheng Dow
CD
Lee
Maykel
M
Cerdas
Jen Ron
JR
Chiang
Yamileth
Y
Angulo
Guillermo
G
León
Juan J
JJ
Calvete
José María
JM
Gutiérrez
D000595:Amino Acid Sequence; D000818:Animals; D000997:Antivenins / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D006801:Humans; D051379:Mice; D009500:Neutralization Tests; D020543:Proteome / Q000032:analysis; D013624:Taiwan; D014757:Viper Venoms / Q000737:chemistry* / Q000276:immunology; D017819:Viperidae
D000997:Antivenins; D020543:Proteome; D014757:Viper Venoms