Phytochemicals isolated from leaves of Chromolaena odorata: impact on viability and clonogenicity of cancer cell lines.
The leaves of Chromolaena odorata (Asteraceae) are exploited extensively in West and Central African ethnopharmacy for the treatment of a wide range of conditions, despite this being a non-native species established in the last 50 years. With the objective of seeking bioactive principles, the nonvolatile compounds, an ethanolic (80% v/v) extract was made and fractionated. From the hexane-soluble fraction, three compounds were isolated. Two of these, 5-hydroxy-7,4'-dimethoxyflavanone and 2'-hydroxy-4,4',5',6'-tetramethoxychalcone, have previously been identified in C. odorata leaves. The third was fully characterised spectroscopically and found to be 1,6-dimethyl-4-(1-methylethyl)naphthalene (cadalene), not previously isolated from the Asteraceae. All three compounds were tested for their cytotoxicity and anticancer properties. 2'-Hydroxy-4,4',5',6'-tetramethoxychalcone was found to be both cytotoxic and anticlonogenic at 20 µm in cell lines Cal51, MCF7 and MDAMB-468, and to act synergistically with the Bcl2 inhibitor ABT737 to enhance apoptosis in Cal51 breast cancer cells.
Elucidation of Biosynthesis by Isotopic Spectrometry Group, CEISAM, University of Nantes-CNRS UMR6230, Nantes, France.
Prevost Bi-Koffi
PB
Kouamé
Camille
C
Jacques
Gustave
G
Bedi
Virginie
V
Silvestre
Denis
D
Loquet
Sophie
S
Barillé-Nion
Richard J
RJ
Robins
Illa
I
Tea
D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D047188:Chalcones / Q000737:chemistry / Q000494:pharmacology; D036604:Chromolaena / Q000737:chemistry*; D044950:Flavanones / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D009281:Naphthalenes / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
D047188:Chalcones; D044950:Flavanones; D009281:Naphthalenes; D010936:Plant Extracts