Structure-based design of a highly constrained nucleic acid analogue: improved duplex stabilization by restricting sugar pucker and torsion angle γ.
Dual conformational restriction: a new, highly constrained modification of the α-L-locked nucleic acid (α-L-LNA) scaffold that locks the sugar furanose ring in an N-type configuration and also restricts rotation around torsion angle γ was synthesized. This new modification increases the thermostability of an oligonucleotide duplex compared to using a single mode of constraint alone.
Department of Chemistry, Université de Montréal, QC, Canada. stephen.hanessian@umontreal.ca
Stephen
S
Hanessian
Benjamin R
BR
Schroeder
Robert D
RD
Giacometti
Bradley L
BL
Merner
Michael
M
Ostergaard
Eric E
EE
Swayze
Punit P
PP
Seth
D008958:Models, Molecular; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D009696:Nucleic Acids; D009841:Oligonucleotides