Proteomic analysis of the cerebrospinal fluid in multiple sclerosis             and neuromyelitis optica patients.
The present study aimed to compare the 2-dimensional (2D) electrophoresis             pattern of the cerebrospinal fluid (CSF) in multiple sclerosis (MS), neuromyelitis             optica (NMO) and control individuals, to identify the proteins with differential             expression and to examine their significance. CSF samples from the three groups             were collected and total protein was isolated and quantified using the Bradford             method. 2D electrophoresis of the samples was conducted using equal amounts of             CSF. In the CSF 2D gel electrophoresis map, 118 points were obtained from the             MS group, 155 points from the NMO group and 350 points from the normal control             group. Non-matching proteins appeared in 14 spots in the MS group and in 45 spots             in the NMO group, and were also expressed in the 2D electrophoresis pattern of             the normal control group. Four differential proteins were identified through the             HD-MS/MS and MASCOT network search. Pre-albumin (PA) was found only in the CSF             2D gel electrophoresis map of the MS patients. Keratin 1 was expressed in the             normal control group, but not in the MS group. The difference in the expression             of keratin 9 in the MS group was twice that in the normal control group. The expression             of keratin 1, keratin 9 and transferrin in the NMO group was twice that in the             normal control group. The expression of PA was found only in the CSF 2D gel electrophoresis             map of the MS patients, and not in the NMO group. Keratin 1 was expressed in the             NMO group, but not in the MS group. The expression of a variety of proteins in             the 2D electrophoresis pattern of CSF was significantly different in the MS, NMO             and control groups. PA, keratin 1, transferrin and keratin 9 are identified as             significantly differentially expressed proteins.
Department of Neurology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, PR China. sfj2001@yeah.net
Shoufeng
S
Jiang
Jiong
J
Wu
Yi
Y
Yang
Jianren
J
Liu
Yao
Y
Ding
Meiping
M
Ding
D000293:Adolescent; D000328:Adult; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D053556:Keratin-1 / Q000378:metabolism; D053534:Keratin-9 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000473:pathology; D009471:Neuromyelitis Optica / Q000134:cerebrospinal fluid* / Q000473:pathology; D011228:Prealbumin / Q000378:metabolism; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D061250:Transferrins / Q000378:metabolism; D055815:Young Adult
D053556:Keratin-1; D053534:Keratin-9; D011228:Prealbumin; D061250:Transferrins