Construction of human naive antibody gene libraries.
Human antibodies are valuable tools for proteome research and diagnostics. Furthermore, antibodies are a rapidly growing class of therapeutic agents, mainly for inflammation and cancer therapy. The first therapeutic antibodies are of murine origin and were chimerized or humanized. The later-developed antibodies are fully human antibodies. Here, two technologies are competing the hybridoma technology using transgenic mice with human antibody gene loci and antibody phage display. The starting point for the selection of human antibodies against any target is the construction of an antibody phage display gene library.In this review we describe the construction of human naive and immune antibody gene libraries for antibody phage display.
Institut für Biochemie und Biotechnologie, Techische Universität Braunschweig, Braunschweig, Germany. m.hust@tu-bs.de
Michael
M
Hust
André
A
Frenzel
Torsten
T
Meyer
Thomas
T
Schirrmann
Stefan
S
Dübel
D000818:Animals; D000906:Antibodies / Q000235:genetics*; D001435:Bacteriophages; D002469:Cell Separation; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D006801:Humans; D015151:Immunoblotting; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D016133:Polymerase Chain Reaction; D057127:Single-Chain Antibodies / Q000235:genetics / Q000276:immunology
D000906:Antibodies; D018076:DNA, Complementary; D057127:Single-Chain Antibodies