Dokdonella immobilis sp. nov., isolated from a batch reactor for the treatment of triphenylmethane dye effluent.
A Gram-staining-negative, non-endospore-forming, non-flagellated, non-motile and rod-shaped bacterium, designated strain LM2-5(T), was isolated from activated sludge in a sequencing batch reactor used for the treatment of triphenylmethane dye effluent. The taxonomy of strain LM2-5(T) was studied by phenotypic, chemotaxonomic and phylogenetic methods. Strain LM2-5(T) was aerobic, heterotrophic and positive for oxidase but negative for catalase activity. It grew at 16-37 °C (optimum 25 °C) and at pH 5.0-8.5 (optimum between pH 6.5 and pH 7.0). NaCl was not obligatory for growth but was tolerated at concentrations up to 2.0 % (w/v) NaCl. The novel strain formed yellow colonies on trypticase soy agar. Cells of strain LM2-5(T) were rods that measured 0.3-0.5 µm in width and 3.0-5.0 µm in length. The predominant respiratory quinone was Q-8. The major fatty acids were iso-C15 : 0 and iso-C17 : 1ω9c. The genomic DNA G+C content was 66.7 mol%. In phylogenetic analyses based on 16S rRNA gene sequences, strain LM2-5(T) clustered with members of the genus Dokdonella and appeared most closely related to Dokdonella koreensis DS-123(T) (96.4 % sequence similarity), Dokdonella fugitiva A3(T) (96.1 %), Dokdonella soli KIS28-6(T) (95.7 %) and Dokdonella ginsengisoli Gsoil 191(T) (95.7 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain LM2-5(T) was considered to represent a novel species of the genus Dokdonella, for which the name Dokdonella immobilis is proposed. The type strain is LM2-5(T) ( = CGMCC 1.7659(T)  = JCM 15763(T)).
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Ying
Y
Liu
Environment Protection Research Institute of Light Industry, Beijing 100089, PR China.
Jing-Hua
JH
Jin
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Hong-Can
HC
Liu
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Zhi-Pei
ZP
Liu
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004396:Coloring Agents / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014320:Triphenylmethyl Compounds / Q000378:metabolism*; D014865:Waste Disposal, Fluid; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
D004396:Coloring Agents; D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014320:Triphenylmethyl Compounds; C046945:triphenylmethane