MRI detects brain reorganization after human umbilical tissue-derived cells (hUTC) treatment of stroke in rat.
Human umbilical tissue-derived cells (hUTC) represent an attractive cell source and a potential technology for neurorestoration and improvement of functional outcomes following stroke. Male Wistar rats were subjected to a transient middle cerebral artery occlusion (tMCAo) and were intravenously administered hUTC (N = 11) or vehicle (N = 10) 48 hrs after stroke. White matter and vascular reorganization was monitored over a 12-week period using MRI and histopathology. MRI results were correlated with neurological functional and histology outcomes to demonstrate that MRI can be a useful tool to measure structural recovery after stroke. MRI revealed a significant reduction in the ventricular volume expansion and improvement in cerebral blood flow (CBF) in the hUTC treated group compared to vehicle treated group. Treatment with hUTC resulted in histological and functional improvements as evidenced by enhanced expression of vWF and synaptophysin, and improved outcomes on behavioral tests. Significant correlations were detected between MRI ventricular volumes and histological lesion volume as well as number of apoptotic cells. A positive correlation was also observed between MRI CBF or cerebral blood volume (CBV) and histological synaptic density. Neurological functional tests were also significantly correlated with MRI ventricular volume and CBV. Our data demonstrated that MRI measurements can detect the effect of hUTC therapy on the brain reorganization and exhibited positive correlation with histological measurements of brain structural changes and functional behavioral tests after stroke. MRI ventricular volumes provided the most sensitive index in monitoring brain remodeling and treatment effects and highly correlated with histological and functional measurements.
Department of Neurology, Henry Ford Health System, Detroit, Michigan, United States of America. qjiang1@hfhs.org
Quan
Q
Jiang
Christine
C
Thiffault
Brian C
BC
Kramer
Guang Liang
GL
Ding
Li
L
Zhang
Siamak P
SP
Nejad-Davarani
Lian
L
Li
Ali S
AS
Arbab
Mei
M
Lu
Brad
B
Navia
Stephen J
SJ
Victor
Klaudyne
K
Hong
Qing Jiang
QJ
Li
Shi Yang
SY
Wang
Yi
Y
Li
Michael
M
Chopp
D000818:Animals; D001522:Behavior, Animal; D001921:Brain / Q000473:pathology*; D017690:Cell Transplantation; D002552:Cerebral Ventricles / Q000473:pathology; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000175:diagnosis* / Q000503:physiopathology / Q000628:therapy*; D013997:Time Factors; D014470:Umbilical Cord / Q000166:cytology*