Microplate analytical method for quinones by pulse photo-irradiation and chemiluminescence detection.
Quinones are widely distributed in nature and have various bioactivities. Besides, quinones are also considered as toxicological intermediates which cause severe dangerous effects. Hereby, a sensitive, simple, and rapid method is reported for quinones determination. The proposed method employed time resolved fluorescence (TRF) microplate reader based chemiluminescent (CL) detection for the first time as a novel approach for measurement. Under pulse photo-irradiation, the unique photochemical characteristic of quinones is exploited to liberate reactive oxygen species (ROS) which reacted with photosensitized CL reagent. L-012, luminol analogue, was selected for its high sensitivity. Under our investigation, para-quinones showed high CL response when compared to ortho-quinones. A linear response was obtained for studied quinone concentrations in the range of 0.05-50 μM for 1,4-naphthquinone and of 0.05-150 μM for 2-methyl-1,4-naphthoquinone (menadione) and 9,10-anthraquinone with detection limit (blank + 3SD) of 0.01 μM. The proposed method allowed the rapid determination of large number of samples in very short time (96 sample/125 s). The proposed method was successfully applied for determination of menadione in spiked human serum.
Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, Japan.
Mohamed Saleh
MS
Elgawish
Chikako
C
Shimomai
Naoya
N
Kishikawa
Kaname
K
Ohyama
Kenichiro
K
Nakashima
Naotaka
N
Kuroda
D006801:Humans; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry; D011809:Quinones / Q000032:analysis*; D017382:Reactive Oxygen Species / Q000737:chemistry; D024483:Vitamin K 3 / Q000097:blood
D011809:Quinones; D017382:Reactive Oxygen Species; D008165:Luminol; D024483:Vitamin K 3