TiO2 nanocrystals electrochemiluminescence quenching by biological enlarged nanogold particles and its application for biosensing.
Electrochemiluminescence (ECL) of TiO(2) nanocrystals with different crystal styles modified fluorine-doped tin oxide (FTO) electrode was investigated in H(2)O(2) solution. The amorphous TiO(2) nanospheres were facilely synthesized by the hydrothermal and condensation method. Crystal TiO(2), namely anatase and rutile, were prepared by calcination of the amorphous TiO(2) nanospheres at 450 and 800°C, respectively. The transmission electron microscope (TEM) and electron diffraction pattern were used to characterize the obtained TiO(2) nanoparticles morphology and the corresponding crystal styles. The electrochemical and ECL behaviors were investigated by cyclic voltammetry. The ECL quenching was observed by introduction of gold nanoparticles. Based on the quenching effect, a sensitive glucose ECL biosensor as a model was fabricated by in-situ growing-up gold seeds in AuCl(4)(-) solution induced by biologically generated H(2)O(2). The linear range to detect glucose is from 5.0×10(-7)M to 4.0×10(-3)M with the limit of detection of 2.5×10(-7)M.
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China. snding@seu.edu.cn
Shou-Nian
SN
Ding
Bu-Hong
BH
Gao
Dan
D
Shan
Yue-Ming
YM
Sun
Serge
S
Cosnier
D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D054879:Halogenation; D006861:Hydrogen Peroxide / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D014025:Titanium / Q000737:chemistry*
C009495:titanium dioxide; D006046:Gold; D006861:Hydrogen Peroxide; D014025:Titanium; D005949:Glucose Oxidase; D005947:Glucose