Superresolution imaging of transcription units on newt lampbrush chromosomes.
We have examined transcription loops on lampbrush chromosomes of the newt Notophthalmus by superresolution microscopy. Because of the favorable, essentially two-dimensional morphology of these loops, an average optical resolution in the x-y plane of about 50 nm was achieved. We analyzed the distribution of the multifunctional RNA-binding protein CELF1 on specific loops. CELF1 distribution is consistent with a model in which individual transcripts are tightly folded and hence closely packed against the loop axis.
Kirchhoff Institute for Physics (KIP), University of Heidelberg, Heidelberg, Germany.
Rainer
R
Kaufmann
Christoph
C
Cremer
Joseph G
JG
Gall
D000818:Animals; D002875:Chromosomes / Q000235:genetics* / Q000648:ultrastructure; D005260:Female; D008856:Microscopy, Fluorescence / Q000379:methods*; D019185:Notophthalmus / Q000235:genetics*; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D016601:RNA-Binding Proteins; D012319:RNA Polymerase II