Voltage controlled nano-injection system for single-cell surgery.
Manipulation and analysis of single cells is the next frontier in understanding processes that control the function and fate of cells. Herein we describe a single-cell injection platform based on nanopipettes. The system uses scanning microscopy techniques to detect cell surfaces, and voltage pulses to deliver molecules into individual cells. As a proof of concept, we injected adherent mammalian cells with fluorescent dyes.
Department of Biomolecular Engineering, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA.
R
R
Adam Seger
Paolo
P
Actis
Catherine
C
Penfold
Michelle
M
Maalouf
Boaz
B
Vilozny
Nader
N
Pourmand
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004560:Electricity; D005347:Fibroblasts / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology / Q000295:instrumentation*
D005456:Fluorescent Dyes