Trace elemental imaging of coralline hydroxyapatite by laser-ablation inductively coupled plasma-mass spectroscopy.
The determination of trace element concentrations, as well as their distribution in different biomaterials aimed for clinical applications, is a challenging task in both the areas of biological and materials research. In this research, LA-ICP-MS was employed for image mapping of the trace element distribution in a hydrothermally converted coralline hydroxyapatite material aimed for tissue-scaffolding applications. Quantification using synthetic matrix-matched standards was successfully applied for the determination and distribution of elements of interest, Sr and Mg, that influences the mechanical and biological properties of hydroxyapatite-based bone graft materials. The results showed that the instrument can successfully analyse trace elements and a relatively good image can be produced that identifies their distribution. The LA-ICP-MS method can provide an easy and effective tool, in the field of biomaterials with respect to distribution of trace elements, to better understand tissue-implant interactions, and will open up a new window for in vitro and in vivo analysis and imaging of different tissues and structures.
School of Medical and Molecular Bioscience, University of Technology Sydney, Broadway, NSW, Australia.
J
J
Chou
C
C
Austin
P
P
Doble
B
B
Ben-Nissan
B
B
Milthorpe
D002516:Ceramics / Q000737:chemistry*; D006882:Hydroxyapatites / Q000737:chemistry*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007834:Lasers; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D011208:Powders; D013054:Spectrophotometry, Atomic / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D014131:Trace Elements / Q000032:analysis*; D014961:X-Ray Diffraction
D006882:Hydroxyapatites; D011208:Powders; D014131:Trace Elements; C119955:coralline hydroxyapatite