Alanine: Glyoxylate aminotransferase 1 is required for mobilization and utilization of triglycerides during infection process of the rice blast pathogen, Magnaporthe oryzae.
The rice blast pathogen, Magnaporthe oryzae has been widely used as a model pathogen to study plant infection-related fungal morphogenesis, such as penetration via appressorium and plant-microbe interactions at the molecular level. Previously, we identified a gene encoding peroxisomal alanine: glyoxylate aminotransferase 1 (AGT1) in M. oryzae and demonstrated that the AGT1 was indispensable for pathogenicity. The AGT1 knockout mutants were unable to penetrate the host plants, such as rice and barley, and therefore were non-pathogenic. The inability of ∆Moagt1 mutants to penetrate the susceptible plants was likely due to the disruption in coordination of the β-oxidation and the glyoxylate cycle resulted from a blockage in lipid droplet mobilization and eventually utilization during conidial germination and appressorium morphogenesis, respectively. Here, we further demonstrate the role of AGT1 in lipid mobilization by in vitro germination assays and confocal microscopy.
Crop Development Centre, University of Saskatchewan, Saskatoon, SK Canada.
Vijai
V
Bhadauria
Sabine
S
Banniza
Albert
A
Vandenberg
Gopalan
G
Selvaraj
Yangdou
Y
Wei
D056229:Fungal Structures / Q000254:growth & development* / Q000378:metabolism; D025301:Hyphae / Q000254:growth & development / Q000378:metabolism; D020082:Magnaporthe / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D009154:Mutation; D012275:Oryza / Q000382:microbiology*; D010084:Oxidation-Reduction; D010935:Plant Diseases / Q000382:microbiology*; D013172:Spores, Fungal / Q000254:growth & development / Q000378:metabolism; D000637:Transaminases / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism*
D014280:Triglycerides; D000637:Transaminases; C019635:Alanine-glyoxylate transaminase