Quantitative chromosome conformation capture.
It is becoming increasingly apparent that chromatin is not randomly folded into the nucleus, but instead is highly organized into specific conformations within the nucleus. One consequence of such higher order structure is that chromatin looping can bring together genomic elements which are separated by several hundreds of kilobases, such as enhancers and promoters, and functionally facilitate their interaction. The Chromosome Conformation Capture (3C) assay is a powerful technique to detect looping structures and assess the probability of interaction between distant genomic elements (1-3). Here we describe the 3C methodology, its power, and limitations, together with the controls and normalization steps required for an accurate analysis.
Department of Oncology, CRUK Cambridge Research Institute, University of Cambridge, Cambridge, UK.
Raffaella
R
Nativio
Yoko
Y
Ito
Adele
A
Murrell
D000818:Animals; D002467:Cell Nucleus / Q000235:genetics; D002843:Chromatin / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D009690:Nucleic Acid Conformation; D011487:Protein Conformation
D002843:Chromatin; D004247:DNA