A flat-coil NMR probe with hydration control of oriented phospholipid bilayer samples.
A novel flat-coil solid-state NMR probe capable of controlling the hydration of oriented phospholipid bilayers in the course of long-term experiments, is described. Perfect hydration control for at least five days of intense radio-frequency pulsing is demonstrated using (31)P NMR of oriented dimyristoylphospha-tidylcholine bilayers. The probe design will be of particular importance for studies of peptides and proteins oriented in lipid bilayers.
Department of Chemistry, University of Aarhus, DK-8000, Aarhus C, Denmark.
N C
NC
Nielsen
P
P
Daugaard
V
V
Langer
J K
JK
Thomsen
S
S
Nielsen
O W
OW
Sørensen
H J
HJ
Jakobsen