Molecular characterization of Giardia duodenalis in children from the Cufada Lagoon Natural Park, Guinea-Bissau.
Molecular characterization of Giardia duodenalis in African countries is relatively scarce. The global understanding of Giardia epidemiology is reinforced when more data are available from highly endemic countries. In the present study, 50 fecal samples collected from children from Guinea-Bissau were screened for Giardia infection. Amplification of the Giardia ssu-rRNA fragment was achieved for 28 samples (28/50, 56.0 %) of which 23 (23/50, 46.0 %) positive samples for Giardia were detected through microscopy. Eighteen samples previous amplified for the ssu-rRNA locus were amplified for the bg gene fragment. Sequence analysis was performed in 26 and 17 samples for the ssu-rRNA and bg gene fragment, respectively. Our results revealed a predominance of assemblage B (22/26, 84.6 %), sequences with high genetic polymorphism among isolates belonging to this assemblage, making impossible the subassemblage determination. Assemblage A was identified in three isolates (3/26, 11.5 %), and our results strongly suggest that two isolates belong to subassemblage A2. This study provides information about G. duodenalis genotypes in a rural area of Guinea-Bissau and may contribute for a better understanding of giardiasis epidemiology in this country.
Unidade de Clínica Tropical e Centro de Malária e Doenças Tropicais-LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal. fsferreira@ihmt.unl.pt
Filipa Santana
FS
Ferreira
S
S
Centeno-Lima
J
J
Gomes
F
F
Rosa
V
V
Rosado
R
R
Parreira
L
L
Cravo
J
J
Atouguia
L
L
Távora Tavira
D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005260:Female; D020459:Genes, rRNA; D014644:Genetic Variation; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology* / Q000469:parasitology*; D006169:Guinea-Bissau / Q000453:epidemiology; D006801:Humans; D007223:Infant; D008297:Male; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S