[Genetic diversity of 21 Fusarium strains in Section Martiella based on ISSR analysis].
In order to understand the genetic difference and phylogenic relationship within and among the Fusarium species in Section Martiella, the genetic diversity of 21 Fusarium strains in the section was examined by inter-simple sequence repeats (ISSR). Fifteen selected ISSR primers were adopted to do amplification, and a total of 239 bands were amplified, among which, 230 (96.2%) were polymorphic, with an average of 15.3 polymorphic bands per primer. The genetic similarity ranged from 0.494 to 0.933, with an average of 0.640. All the test strains were clustered into two groups at genetic similarity of 0.593. The strains 1-17 were grouped into IG-I, belonging to Fusarium solani and F. solani var. coeruleum, while the strains 18-21 were grouped into IG-II, belonging to F. ventricosum. All the 21 strains could be entirely distinguished at genetic similarity of 0. 933. The SSR loci in the Fusarium genomes were rich in polymorphism. The ISSR grouping had definite correlation with the species classification, but less correlation with the geographic origin of the strains. Within the same ISSR groups, there existed definite correlation between the genetic similarity and the geographic origin of the strains. Within the same species collected from the same regions and same host plants, there existed definite genetic difference among the strains of the same species.
College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China. lxf1309@163.com
Xin-Feng
XF
Li
Guang-Ming
GM
Zhang
Yin-Dong
YD
Chang
Jian-Ming
JM
Wang
Rui-Qian
RQ
Li
Ya-Li
YL
Li
D005670:Fusarium / Q000145:classification / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics*; D020071:Interspersed Repetitive Sequences / Q000235:genetics*; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics*