Inhibition of ENNG-induced pyloric stomach and small intestinal carcinogenesis in mice by high temperature- and pressure-treated garlic.
High temperature- and pressure-treated garlic (HTPG) has been shown to have enhanced antioxidative activity and polyphenol contents. Previously, we reported that HTPG inhibited 1,2-dimethylhydrazine-induced mucin depleted foci (premalignant lesions) and O6-methylguanine DNA adduct formation in the rat colorectum. In the present study, we investigated the modifying effects of HTPG on N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG)- induced pyloric stomach and small intestinal carcinogenesis in mice. Male C57BL/6 mice were given ENNG (100 mg/l) in drinking water for the first 4 weeks, then a basal diet or diet containing 2% or 5% HTPG for 30 weeks. The incidence and multiplicity of pyloric stomach and small intestinal (duodenal and jejunal) tumors in the 2% HTPG group (but not in the 5% HTPG group) were significantly lower than those in the control group. Cell proliferation of normal-appearing duodenal mucosa was assessed by MIB-5 immunohistochemistry and shown to be significantly lower with 2% HTPG (but again not 5% HTPG) than in controls. These results in dicate that HTPG, at 2% in the diet, inhibited ENNG-induced pyloric stomach and small intestinal (especially duodenal) tumorigenesis in mice, associated with suppression of cell proliferation.
Fujita Memorial Nanakuri Institute.
Takaaki
T
Kaneko
Kan
K
Shimpo
Takeshi
T
Chihara
Hidehiko
H
Beppu
Akiko
A
Tomatsu
Masanori
M
Shinzato
Takamasa
T
Yanagida
Tsutomu
T
Ieike
Shigeru
S
Sonoda
Akihiko
A
Futamura
Akihiro
A
Ito
Takashi
T
Higashiguchi
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D049109:Cell Proliferation; D005737:Garlic / Q000737:chemistry*; D006358:Hot Temperature; D007124:Immunoenzyme Techniques; D007414:Intestinal Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D007421:Intestine, Small / Q000187:drug effects / Q000473:pathology*; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000031:analogs & derivatives* / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D011312:Pressure; D013274:Stomach Neoplasms / Q000139:chemically induced / Q000517:prevention & control*
D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; C013525:ENNG