[Expression and molecular evolution of recombinant acetylcholinesterase for detection of pesticide residues: a review].
Acetylcholinesterase (AChE) plays a key role in the pesticide determination. However, the extraction of AChE from natural materials has the disadvantages of low yield, complex purification and poor stability. Therefore, the preparation of recombinant AChE with high performance becomes the hot topic of researchers in recent years. In this article we summarize the progress in the expression of recombinant AChE and the improvement of its analytical characteristic. Finally, we point out that the directed evolution strategy combined with surface display technology is the future trend on improving recombinant AChE activity.
Guangdong Provincial Key Laboratory of Food Quality and Safety, Food College of South China Agricultural University, Guangzhou 510640, Guangdong, China.
Jiexian
J
Dong
Zhenfeng
Z
Li
Hongtao
H
Lei
Yongsheng
Y
He
Hong
H
Wang
Yuanming
Y
Sun
D000110:Acetylcholinesterase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D061505:Cell Surface Display Techniques; D002800:Cholinesterase Inhibitors / Q000032:analysis; D019143:Evolution, Molecular; D005822:Genetic Vectors / Q000235:genetics*; D010573:Pesticide Residues / Q000032:analysis*; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics*
D002800:Cholinesterase Inhibitors; D010573:Pesticide Residues; D011994:Recombinant Proteins; D000110:Acetylcholinesterase