[Bioinformatics prediction of egA31 recombinant antigen epitopes of Echinococcus granulosus].
Specific primers were designed and synthesized based on the reported EgA31 gene of Echinococcus granulosus (GenBank Accession No. AF067807). Total RNA was extracted from E. granulosus and its EgA31 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The PCR product was purified and cloned into plasmid pUCM-T, then transformed into Escherichia coli DH5alpha. The recombinant plasmids were screened and identified by digestion with restriction enzyme and PCR amplification. The positive recombinant plasmid pUCM-T/EgA31 was confirmed by sequencing and homology comparison. Five parameters and methods were used to predict B-cell epitopes in amino acid sequence of EgA31. The amplified DNA fragment (636 bp) had an identity of 100% with the EgA31 gene sequence of E. granulosus. B-cell and T-cell epitopes of EgA31 were probably at or adjacent to 32-79, 79-95, 105-124 and 141-154 in its amino acid sequence.
Medical Research Center and Xinjiang Key Lab, the First Affiliated Hospital of Xinjiang Medical University, Urunmqi 830011, China.
Yu-jiao
YJ
Li
Jing
J
Yang
Hui
H
Zhao
Hai-ying
HY
Jia
Li-na
LN
Zhang
Xiao-xia
XX
Liu
Xiu-min
XM
Ma
Hao
H
Wen
Jian-bing
JB
Ding
D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D019295:Computational Biology; D048209:Echinococcus granulosus / Q000235:genetics* / Q000276:immunology*; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000276:immunology
D000947:Antigens, Helminth; C401864:EgA31 protein, Echinococcus granulosus; D000939:Epitopes; D011993:Recombinant Fusion Proteins