Decreased expression of the carboxyl terminus of heat shock cognate             70 interacting protein in human gastric cancer and its clinical significance.
The carboxyl terminus of heat shock cognate 70 interacting protein (CHIP)             is an E3 ubiquitin ligase, which can promote ubiquitylation and degradation of             many tumor-related proteins. However, the expression of CHIP in human gastric             cancer has not been investigated. In this study, the mRNA and protein levels of             CHIP expression in 53 cases of gastric cancer and matched normal tissues were             determined by quantitative real-time PCR, western blotting and immunohistochemistry.             We showed that CHIP was registered from basal to middle portions of normal gastric             mucosa. CHIP expression was notably decreased or lost in human gastric cancer             samples compared with the matched normal non-cancer samples. The correlations             between CHIP downregulation and the clinicopathological characteristics were also             evaluated. The expression of CHIP was significantly lower in the gastric cancer             samples compared to the matched normal samples at both mRNA and protein levels             (P<0.05 and P<0.05, respectively). More importantly, the downregulation             of CHIP was correlated with TNM stage (P=0.048) and lymph node metastasis (P=0.010)             at the mRNA levels. In addition, the downregulation of CHIP was correlated with             lymph node metastasis (P=0.021) and tumor differentiation (P=0.009) at the protein             levels. Taken together, at both mRNA and protein levels, the decreased expression             of CHIP was correlated with lymph node metastasis. Furthermore, our study suggests             that a negative correlation exists between CHIP expression and tumor malignancy             in human gastric cancer.
Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
Lei
L
Gan
Dong-Bo
DB
Liu
Hai-Feng
HF
Lu
Guo-Xian
GX
Long
Qi
Q
Mei
Guang-Yuan
GY
Hu
Hong
H
Qiu
Guo-Qing
GQ
Hu
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D044767:Ubiquitin-Protein Ligases / Q000235:genetics* / Q000378:metabolism*; D055815:Young Adult
C467728:STUB1 protein, human; D044767:Ubiquitin-Protein Ligases