Hemodialysis decreases serum brain-derived neurotrophic factor concentration in humans.
In the present study we have evaluated the effect of a single hemodialysis session on the brain-derived neurotrophic factor levels in plasma [BDNF](pl) and in serum [BDNF](s) as well as on the plasma isoprostanes concentration [F(2) isoprostanes](pl), plasma total antioxidant capacity (TAC) and plasma cortisol levels in chronic kidney disease patients. Twenty male patients (age 69.8 ± 2.9 years (mean ± SE)) with end-stage renal disease undergoing maintenance hemodialysis on regular dialysis treatment for 15-71 months participated in this study. A single hemodialysis session, lasting 4.2 ± 0.1 h, resulted in a decrease (P = 0.014) in [BDNF](s) by ~42 % (2,574 ± 322 vs. 1,492 ± 327 pg ml(-1)). This was accompanied by an increase (P < 10(-4)) of [F(2)-Isoprostanes](pl) (38 ± 3 vs. 116 ± 16 pg ml(-1)), decrease (P < 10(-4)) in TAC (1,483 ± 41 vs. 983 ± 35 trolox equivalents, μmol l(-1)) and a decrease (P = 0.004) in plasma cortisol level (449.5 ± 101.2 vs. 315.3 ± 196.3 nmol l(-1)). No changes (P > 0.05) in [BDNF](pl) and the platelets count were observed after a single dialysis session. Furthermore, basal [BDNF](s) in the chronic kidney disease patients was significantly lower (P = 0.03) when compared to the age-matched control group (n = 23). We have concluded that the observed decrease in serum BDNF level after hemodialysis accompanied by elevated [F(2)-Isoprostanes](pl) and decreased plasma TAC might be caused by enhanced oxidative stress induced by hemodialysis.
Department of Physiology and Biochemistry, Faculty of Rehabilitation, University School of Physical Education Kraków, al. Jana Pawla II 78, 31-571 Kraków, Poland. jerzy.zoladz@awf.krakow.pl
Jerzy A
JA
Zoladz
Michał
M
Śmigielski
Joanna
J
Majerczak
Łukasz R
ŁR
Nowak
Justyna
J
Zapart-Bukowska
Olgierd
O
Smoleński
Jan
J
Kulpa
Krzysztof
K
Duda
Joanna
J
Drzewińska
Grzegorz
G
Bartosz
D000368:Aged; D000369:Aged, 80 and over; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood*; D028441:F2-Isoprostanes / Q000097:blood; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D006435:Renal Dialysis
D019208:Brain-Derived Neurotrophic Factor; D028441:F2-Isoprostanes; D006854:Hydrocortisone