Quantifying monocyte infiltration in response to intradermal tetanus toxoid injection.
OBJECTIVE
To characterize monocyte response in a delayed-type hypersensitivity reaction to intradermal tetanus toxoid (TT) injection.


METHODS
Men with positive serum anti-tetanus titers were stratified by last TT vaccination. Subjects were administered three intradermal injections of TT and one saline control on the same side of the back. Skin biopsies were taken post-injection. After 2 weeks, the procedure was repeated on the contralateral side.


RESULTS
Men who received TT booster vaccination 1 month before the study showed greater reproducibility and lower variability in monocyte responses than those who were not revaccinated. Monocyte concentration in subjects re-vaccinated within 1 month of study start appeared maximal at 48 h post-injection.


CONCLUSIONS
This assay represents a novel approach that allows for quantification of dermal monocyte/macrophage influx. This clinical methodology has potential utility in the pharmacodynamic evaluation of therapies targeting inflammatory disorders, which involve monocyte tissue recruitment, like the delayed-type hypersensitivity response.
Merck Sharp & Dohme Corp., Whitehouse Station, NJ, USA.
Joanna Z
JZ
Peng
David E
DE
Gutstein
Lisa
L
Beck
Lisa
L
Hickey
Carolyn M
CM
Hustad
Smita
S
Abbi
Ajay
A
Nirula
Julie
J
DeMartino
Paul
P
Rothenberg
Keith
K
Gottesdiener
Daniel M
DM
Bloomfield
John A
JA
Wagner
D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D002465:Cell Movement; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology / Q000473:pathology; D007271:Injections, Intradermal; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology* / Q000276:immunology; D015203:Reproducibility of Results; D012867:Skin / Q000378:metabolism / Q000473:pathology; D013745:Tetanus Toxoid / Q000276:immunology*; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D013745:Tetanus Toxoid; C067980:CD68 antigen, human; D000097382:CD68 Molecule