A newly anti-Streptococcus suis bacteriocin producing strain from unweaned piglets fecal matter: isolation, preliminary identification, and optimization of medium composition for enhanced bacteriocin production.
A newly isolated anti-Streptococcus suis bacteriocin-producing strain LPL1-5 was obtained from healthy unweaned piglets' fecal matter, and was designated as Lactobacillus pentosus LPL1-5 based on morphology, biochemical properties, and 16S rDNA sequencing analysis. The medium composition for enhanced bacteriocin production by L. pentosus LPL1-5 was optimized by statistical methodology. Yeast extract, K(2)HPO(4)·3H(2)O, and MnSO(4)·H(2)O were identified as significant components influencing pentocin LPL1-5 production using the Plackett-Burman method. Response surface methodology was applied for further optimization. The concentrations of medium components for enhanced pentocin LPL1-5 production were as follows (g/L): lactose 20.00, tryptone 10.00, beef extract 10.00, yeast extract 14.00, MnSO(4)·H(2)O 0.84, K(2)HPO(4)·3H(2)O 4.92, triammonium citrate 2.00, Na-acetate 5.00, MgSO(4)·7H(2)O 0.58, Tween 80 1.00. Under the optimized condition, a value of 3154.65 ± 27.93 IU/mL bacteriocin activity was achieved, which was 4.2-fold that of the original medium.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Xiangmei
X
Zhang
Xiaoyuan
X
Chang
Guorong
G
Liu
Pengpeng
P
Wu
Pinglan
P
Li
D000465:Algorithms; D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification*; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D005243:Feces / Q000382:microbiology; D007778:Lactobacillus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017011:Streptococcus suis / Q000187:drug effects / Q000254:growth & development; D013552:Swine
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003470:Culture Media; D012336:RNA, Ribosomal, 16S