A novel bioactive peptide from yoghurts modulates expression of the gel-forming MUC2 mucin as well as population of goblet cells and Paneth cells along the small intestine.
Several studies demonstrated that fermented milks may provide a large number of bioactive peptides into the gastrointestinal tract. We previously showed that beta-casomorphin-7, an opioid-like peptide produced from bovine β-casein, strongly stimulates intestinal mucin production in ex vivo and in vitro models, suggesting the potential benefit of milk bioactive peptides on intestinal protection. In the present study, we tested the hypothesis that the total peptide pool (TPP) from a fermented milk (yoghurt) may act on human intestinal mucus-producing cells (HT29-MTX) to induce mucin expression. Our aim was then to identify the peptide(s) carrying the biological activity and to study its impact in vivo on factors involved in gut protection after oral administration to rat pups (once a day, 9 consecutive days). TPP stimulated MUC2 and MUC4 gene expression as well as mucin secretion in HT29-MTX cells. Among the four peptide fractions that were separated by preparative reversed-phase high-performance liquid chromatography, only the C2 fraction was able to mimic the in vitro effect of TPP. Interestingly, the sequence [94-123] of β-casein, present only in C2 fraction, also regulated mucin production in HT29-MTX cells. Oral administration of this peptide to rat pups enhanced the number of goblet cells and Paneth cells along the small intestine. These effects were associated with a higher expression of intestinal mucins (Muc2 and Muc4) and of antibacterial factors (lysozyme, rdefa5). We conclude that the peptide β-CN(94-123) present in yoghurts may maintain or restore intestinal homeostasis and could play an important role in protection against damaging agents of the intestinal lumen.
INRA USC1235, INSERM U1060, CarMeN laboratory, F-69621 Villeurbanne, France. pascale.plaisancie@inserm.fr
Pascale
P
Plaisancié
Jean
J
Claustre
Monique
M
Estienne
Gwénaële
G
Henry
Rachel
R
Boutrou
Armelle
A
Paquet
Joëlle
J
Léonil
D000595:Amino Acid Sequence; D000818:Animals; D002364:Caseins / Q000494:pharmacology; D002460:Cell Line / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D020397:Goblet Cells / Q000187:drug effects*; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects; D007421:Intestine, Small / Q000166:cytology* / Q000187:drug effects*; D008969:Molecular Sequence Data; D055262:Mucin-2 / Q000235:genetics / Q000378:metabolism*; D055264:Mucin-4 / Q000235:genetics / Q000378:metabolism; D009077:Mucins / Q000378:metabolism; D019879:Paneth Cells / Q000187:drug effects*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015014:Yogurt
D002364:Caseins; C524625:MUC2 protein, human; C524658:MUC4 protein, human; D055262:Mucin-2; D055264:Mucin-4; D009077:Mucins; D010455:Peptides