[Comparative study on the HPV infection rate of different esophageal squamous cell carcinoma in Anyang China].
OBJECTIVE
Comparative and statistical analysis the HPV infection rate between fresh tissue and Paraffin-embedded Specimens of esophageal squamous cell carcinoma,and comparative the testing results with others regions.


METHODS
Extracted the total DNA from the novel fresh tissue and Paraffin-embedded Specimens; Detected the DNA by PCR with universal primer and Detected the HPV type with human papilloma virus nucleic acid amplification-based typing detection reagent kit (Hybribio); Compared the statistical result from the different specimens; analyzed the result between different region.


RESULTS
HPV infection rate of fresh tissue is 82.6% with HPV16 (34.8%) and HPV18 (34.8%), and paraffin-embedded specimens is 78.2% with HPV16 (30.4%) and HPV18 (17.4%).


CONCLUSIONS
The results provides the first evidence that there wasn't noticeable difference between HPV infection rate of the two specimens. So broader specimen source could be used for HPV testing.
The College of Life Science, Shandong Normal University, Jinan 250014, China.
Peng
P
Qu
Jin-Tao
JT
Li
Li-Dong
LD
Wang
Yi
Y
Zeng
Xiu-Sheng
XS
Chu
D036022:Capsid Proteins / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D004938:Esophageal Neoplasms / Q000821:virology*; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000032:analysis; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology*; D016133:Polymerase Chain Reaction
D036022:Capsid Proteins; D004279:DNA, Viral; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral