A versatile assay for monitoring in vivo-like transendothelial migration of neutrophils.
Spatiotemporal analysis of the inflammatory response has been limited by the difficulties of in vivo imaging and reconstitution of inflammation in vitro. Here, we present a novel method for establishing in vivo-like inflammatory models in a microfluidic device and quantitatively measuring the three-dimensional transmigration of neutrophils during the inflammatory process. This enabled us to concurrently characterize transendothelial migration behaviors of neutrophils under the influence of various inflammatory stimuli.
School of Mechanical Engineering, Korea University, South Korea.
Sewoon
S
Han
Ji-Jing
JJ
Yan
Yoojin
Y
Shin
Jessie J
JJ
Jeon
Jihee
J
Won
Hyo Eun
HE
Jeong
Roger D
RD
Kamm
Young-Joon
YJ
Kim
Seok
S
Chung
D006801:Humans; D007249:Inflammation / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D008954:Models, Biological; D009504:Neutrophils / Q000166:cytology* / Q000378:metabolism*; D057705:Transendothelial and Transepithelial Migration