Analysis of hydrolyzable tannins and other phenolic compounds in emblic leafflower (Phyllanthus emblica L.) fruits by high performance liquid chromatography-electrospray ionization mass spectrometry.
Phenolic compounds were extracted from dried emblic leafflower (Phyllanthus emblica L.) fruits with methanol and separated by Sephadex LH-20 column chromatography. The raw extracts and fractions were analyzed with HPLC coupled with diode array UV spectroscopy, electrospray ionization mass spectrometry, and tandem mass spectrometry. Mucic acid gallate, mucic acid lactone gallate, monogalloylglucose, gallic acid, digalloylglucose, putranjivain A, galloyl-HHDP-glucose, elaeocarpusin, and chebulagic acid were suggested to be the most abundant compounds in the crude methanol extracts of the fruits. In addition, 144 peaks were detected, of which 67 were tentatively identified mostly as ellagitannins, flavonoids, and simple gallic acid derivatives in the fractions. The results indicated the presence of neochebulagic acid, isomers of neochebuloyl galloylglucose, chebuloyl neochebuloyl galloylglucose, ellagic acid glycosides, quercetin glycosides, and eriodictyol coumaroyl glycosides in the fruits. The study provides a systematic report of the retention data and characteristics of UV, MS, and MS/MS spectra of the phenolic compounds in the fruits of emblic leafflower. The fruits of two varieties (Ping Dan No 1 and Fruity) from Guangxi Province differed from those of wild Tian Chuan emblic leafflower from Fujian Province in the content and profile of phenolic compounds.
Department of Biochemistry and Food Chemistry, University of Turku, Turku, Finland. baoru.yang@utu.fi
Baoru
B
Yang
Maaria
M
Kortesniemi
Pengzhan
P
Liu
Maarit
M
Karonen
Juha-Pekka
JP
Salminen
D002681:China; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D005707:Gallic Acid / Q000032:analysis; D047348:Hydrolyzable Tannins / Q000032:analysis*; D010636:Phenols / Q000032:analysis*; D029842:Phyllanthus emblica / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D005419:Flavonoids; D047348:Hydrolyzable Tannins; D010636:Phenols; D010936:Plant Extracts; D005707:Gallic Acid