Patch-clamp protocols to study cell ionic homeostasis under saline conditions.
The patch-clamp technique was designed to measure any electrogenic transport across the whole cell and organelle (vacuolar) membranes and excised membrane patches. Here, we describe preparation of protoplasts and vacuoles, as well as patch-clamp assays, to detect the functional expression of K(+) and cation channels of plasma membrane and tonoplast, as well as plasma membrane anion channels and vacuolar and plasma membrane H(+) pumps. All of these contribute to the intracellular ionic homeostasis under saline conditions.
Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, Colima, Mexico.
Ana María
AM
Velarde-Buendía
René Alberto
RA
Enríquez-Figueroa
Igor
I
Pottosin
D006706:Homeostasis; D001467:Hordeum / Q000166:cytology / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism; D017136:Ion Transport; D007477:Ions / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D018408:Patch-Clamp Techniques / Q000379:methods*; D059828:Plant Cells / Q000378:metabolism*; D018517:Plant Roots / Q000166:cytology / Q000378:metabolism; D017494:Proton Pumps / Q000378:metabolism; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism; D012639:Seeds / Q000254:growth & development; D012965:Sodium Chloride; D012996:Solutions; D014617:Vacuoles / Q000378:metabolism
D007473:Ion Channels; D007477:Ions; D026901:Membrane Transport Proteins; D017494:Proton Pumps; D012996:Solutions; D012965:Sodium Chloride