A single locus is responsible for salinity tolerance in a Chinese landrace barley (Hordeum vulgare L.).
BACKGROUND
Salinity and waterlogging are two major abiotic stresses severely limiting barley production. The lack of a reliable screening method makes it very hard to improve the tolerance through breeding programs.


METHODS
This work used 188 DH lines from a cross between a Chinese landrace variety, TX9425 (waterlogging and salinity tolerant), and a Japanese malting barley, Naso Nijo (waterlogging and salinity sensitive), to identify QTLs associated with the tolerance.


RESULTS
Four QTLs were found for waterlogging tolerance. The salinity tolerance was evaluated with both a hydroponic system and in potting mixture. In the trial with potting mixture, only one major QTL was identified to associate with salinity tolerance. This QTL explained nearly 50% of the phenotypic variation, which makes it possible for further fine mapping and cloning of the gene. This QTL was also identified in the hydroponic experiment for different salt-related traits. The position of this QTL was located at a similar position to one of the major QTLs for waterlogging tolerance, indicating the possibility of similar mechanisms controlling both waterlogging and salinity tolerance.


CONCLUSIONS
The markers associated with the QTL provided a unique opportunity in breeding programs for selection of salinity and waterlogging tolerance.
Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology and Barley Research Institution of Yangzhou University, Yangzhou, China.
Rugen
R
Xu
Junmei
J
Wang
Chengdao
C
Li
Peter
P
Johnson
Chao
C
Lu
Meixue
M
Zhou
D003433:Crosses, Genetic; D001467:Hordeum / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics; D055049:Salt Tolerance / Q000235:genetics*