Oceanicella actignis gen. nov., sp. nov., a halophilic slightly thermophilic member of the Alphaproteobacteria.
Two isolates, designated PRQ-67(T) and PRQ-68, with an optimum growth temperature of about 50°C, growth range in medium containing between 1 and 9% NaCl and an optimum pH for growth between 7.5 and 8.0, were recovered from a shallow marine hot spring on a beach, Praia do Fogo, at Ribeira Quente, on the Island of São Miguel in the Azores. Comparisons of 16S rRNA gene sequences show these strains to be most closely related (93.1-94.7% similarity) to species of the genus Amaricoccus, within the family Rhodobacteraceae. Strains are non-pigmented and form non-motile pleomorphic cells that stain Gram-negative, are aerobic, oxidase and catalase positive. The major fatty acids are C(18:1)ω7c and C(18:1)ω7c 11-methyl. Ubiquinone 10 is the major respiratory quinone. Major polar lipids are phosphatidylcholine, phosphatidylglycerol and one aminolipid. Based on 16S rRNA gene sequence analysis, physiological and biochemical characteristics we describe a new species of a novel genus represented by strain PRQ-67(T) (=DSM 22673(T)=LMG 25334(T)) for which we propose the name Oceanicella actignis.
Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal. luciana@cnc.uc.pt
Luciana
L
Albuquerque
Fred A
FA
Rainey
M Fernanda
MF
Nobre
Milton S
MS
da Costa
D001394:Azores; D002855:Chromatography, Thin Layer; D005798:Genes, Bacterial / Q000235:genetics; D045482:Hot Springs / Q000382:microbiology; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012623:Seawater / Q000382:microbiology
D008055:Lipids; D012336:RNA, Ribosomal, 16S