Lack of significance of the BRCA2 promoter methylation status in different genotypes of the MTHFR a1298c polymorphism in ovarian cancer cases in Iran.
OBJECTIVE
Promoter methylation, which can be regulated by MTHFR activity, is associated with silencing of genes. In this study we evaluated the methylation status (type) of the BRCA2 promoter in ovarian cancer patients carrying different genotypes of the MTHFR gene (A or C polymorphisms at position 1298).


METHODS
The methylation type of the BRCA2 promoter was evaluated using bisulfate-modified DNA in methylation- specific PCR and the MTHFRa1278c polymorphism was assessed by PCR-RFLP.


RESULTS
Analysis of the BRCA2 promoter methylation type of cases showed that 7 out of 60 cases (11.7%) were methylated while the remaining 53 (88.3%) were unmethylated. In methylated cases, one out of the 7 cases had a CC genotype and the remaining 6 methylated cases had an AC genotype. The AA genotype was absent. In unmethylated cases, 34, 18, and one out of these had AC, AA and CC genotype, respectively.


CONCLUSIONS
There was no significant relationship between the methylation types of the BRCA2 promoter in different genotypes of MTHFRa1298c polymorphism in ovarian cancer; p=0.255. There was no significant relation between the methylation types of the BRCA2 promoter in different genotypes of the MTHFRa1298c polymorphism in ovarian cancer.
Department of Anatomy, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. shebani54@yahoo.com
Ahmad Shabanizadeh
AS
Darehdori
Mehdi Nikbakht
MN
Dastjerdi
Hajar
H
Dahim
Mohammadreza
M
Slahshoor
Zahra
Z
Babazadeh
Mohammad Mohsen
MM
Taghavi
Zahra
Z
Taghipour
Hamidreza
H
Gaafarineveh
D024682:BRCA2 Protein / Q000235:genetics*; D016022:Case-Control Studies; D019175:DNA Methylation; D005260:Female; D005838:Genotype; D006801:Humans; D007492:Iran; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*
D024682:BRCA2 Protein; C551750:BRCA2 protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2)