Co-regulation of cell polarization and migration by caveolar proteins PTRF/Cavin-1 and caveolin-1.
Caveolin-1 and caveolae are differentially polarized in migrating cells in various models, and caveolin-1 expression has been shown to quantitatively modulate cell migration. PTRF/cavin-1 is a cytoplasmic protein now established to be also necessary for caveola formation. Here we tested the effect of PTRF expression on cell migration. Using fluorescence imaging, quantitative proteomics, and cell migration assays we show that PTRF/cavin-1 modulates cellular polarization, and the subcellular localization of Rac1 and caveolin-1 in migrating cells as well as PKCα caveola recruitment. PTRF/cavin-1 quantitatively reduced cell migration, and induced mesenchymal epithelial reversion. Similar to caveolin-1, the polarization of PTRF/cavin-1 was dependent on the migration mode. By selectively manipulating PTRF/cavin-1 and caveolin-1 expression (and therefore caveola formation) in multiple cell systems, we unveil caveola-independent functions for both proteins in cell migration.
The University of Queensland Diamantina Institute, The University of Queensland, Brisbane, Queensland, Australia.
Michelle M
MM
Hill
Noor Huda
NH
Daud
Cho Sanda
CS
Aung
Dorothy
D
Loo
Sally
S
Martin
Samantha
S
Murphy
Debra M
DM
Black
Rachael
R
Barry
Fiona
F
Simpson
Libin
L
Liu
Paul F
PF
Pilch
John F
JF
Hancock
Marie-Odile
MO
Parat
Robert G
RG
Parton
D000818:Animals; D015153:Blotting, Western; D051242:Caveolin 1 / Q000378:metabolism*; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology*; D002633:Chemotaxis / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D041681:NIH 3T3 Cells; D009479:Neuropeptides / Q000378:metabolism; D016601:RNA-Binding Proteins; D020744:rac GTP-Binding Proteins / Q000378:metabolism; D020830:rac1 GTP-Binding Protein
D051242:Caveolin 1; C112745:Cavin1 protein, mouse; D008565:Membrane Proteins; D009479:Neuropeptides; D016601:RNA-Binding Proteins; C484602:Rac1 protein, mouse; D020744:rac GTP-Binding Proteins; D020830:rac1 GTP-Binding Protein