Retinal digest preparation: a method to study diabetic retinopathy.
Retinal digestion is a commonly used method for studying experimental diabetic retinopathy in animal models. The method allows to assess qualitatively and quantitatively the morphology of the retinal vasculature, including characteristics of endothelial cells and pericytes. The digestion method uses the enzyme trypsin and enables the precise evaluation of venolar and arteriolar diameters, endothelial cell and pericyte numbers, and the formation of acellular capillaries.
5th Medical Department, Universitätsmedizin Mannheim, University of Heidelberg, Mannheim, Germany.
Nadine
N
Dietrich
Hans-Peter
HP
Hammes
D000818:Animals; D003930:Diabetic Retinopathy / Q000473:pathology*; D006801:Humans; D051379:Mice; D008853:Microscopy / Q000379:methods*; D020286:Pericytes / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D012160:Retina / Q000378:metabolism / Q000473:pathology*; D012171:Retinal Vessels / Q000378:metabolism / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation / Q000379:methods; D014357:Trypsin / Q000378:metabolism
D014357:Trypsin