Altered neurochemical profile after traumatic brain injury: (1)H-MRS biomarkers of pathological mechanisms.
Specific neurochemicals measured with proton magnetic resonance spectroscopy ((1)H-MRS) may serve as biomarkers of pathological mechanism in the brain. We used high field in vivo (1)H-MRS to measure a detailed neurochemical profile after experimental traumatic brain injury (TBI) in rats. We characterized neurochemical changes in the contused cortex and the normal-appearing perilesional hippocampus over a time course from 1 hour to 2 weeks after injury. We found significant changes in 19 out of 20 neurochemicals in the cortex, and 9 out of 20 neurochemicals in the hippocampus. These changes provide evidence of altered cellular metabolic status after TBI, with specific compounds proposed to reflect edema, excitotoxicity, neuronal and glial integrity, mitochondrial status and bioenergetics, oxidative stress, inflammation, and cell membrane disruption. Our results support the utility of (1)H-MRS for monitoring cellular mechanisms of TBI pathology in animal models, and the potential of this approach for preclinical evaluation of novel therapies.
Hoglund Brain Imaging Center, University of Kansas Medical Center, Kansas City, Kansas 66160, USA. jharris2@kumc.edu
Janna L
JL
Harris
Hung-Wen
HW
Yeh
In-Young
IY
Choi
Phil
P
Lee
Nancy E
NE
Berman
Russell H
RH
Swerdlow
Sorin C
SC
Craciunas
William M
WM
Brooks
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001923:Brain Chemistry; D001930:Brain Injuries / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D011522:Protons; D051381:Rats; D011916:Rats, Inbred F344
D015415:Biomarkers; D011522:Protons