Hirschia litorea sp. nov., isolated from seashore sediment, and emended description of the genus Hirschia.
A Gram-negative, non-spore-forming, non-flagellated, coccoid-, oval- or rod-shaped strain, designated M-M23(T), was isolated from seashore sediment at Geoje island, South Korea. Strain M-M23(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain M-M23(T) clustered with the type strains of the two species of the genus Hirschia, with which it exhibited 97.6-98.1 % 16S rRNA gene sequence similarity. Sequence similarity with the type strains of other recognized species was <90.8 %. Strain M-M23(T) contained Q-10 as the predominant ubiquinone and C18 : 1ω7c and C16 : 0 as the major fatty acids. The major polar lipids of strain M-M23(T) were phosphatidylglycerol and two unidentified lipids. The DNA G+C content of strain M-M23(T) was 45.4 mol%. DNA-DNA relatedness between the isolate and Hirschia baltica DSM 5838(T) and Hirschia maritima JCM 14974(T) was 22±7.2 and 14±5.6 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain M-M23(T) is separate from the other described members of the genus Hirschia. On the basis of the data presented, strain M-M23(T) is considered to represent a novel species of the genus Hirschia, for which the name Hirschia litorea sp. nov. is proposed. The type strain is M-M23(T) ( = KCTC 32081(T)  = CCUG 62793(T)). An emended description of the genus Hirschia is also provided.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Sooyeon
S
Park
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Jung-Hoon
JH
Yoon
D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010715:Phosphatidylglycerols / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010715:Phosphatidylglycerols; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10