Interaction of brassinosteroids and polyamines enhances copper stress tolerance in raphanus sativus.
Brassinosteroids (BRs) and polyamines (PAs) regulate various responses to abiotic stress, but their involvement in the regulation of copper (Cu) homeostasis in plants exposed to toxic levels of Cu is poorly understood. This study provides an analysis of the effects of exogenously applied BRs and PAs on radish (Raphanus sativus) plants exposed to toxic concentrations of Cu. The interaction of 24-epibrassinolide (EBR, an active BR) and spermidine (Spd, an active PA) on gene expression and the physiology of radish plants resulted in enhanced tolerance to Cu stress. Results indicated that the combined application of EBR and Spd modulated the expression of genes encoding PA enzymes and genes that impact the metabolism of indole-3-acetic acid (IAA) and abscisic acid (ABA) resulting in enhanced Cu stress tolerance. Altered expression of genes implicated in Cu homeostasis appeared to be the main effect of EBR and Spd leading to Cu stress alleviation in radish. Ion leakage, in vivo imaging of H(2)O(2), comet assay, and improved tolerance of Cu-sensitive yeast strains provided further evidence for the ability of EBR and Spd to improve Cu tolerance significantly. The study indicates that co-application of EBR and Spd is an effective approach for Cu detoxification and the maintenance of Cu homeostasis in plants. Therefore, the use of these compounds in agricultural production systems should be explored.
Department of Horticulture, Zhejiang University Hangzhou 310058, Zhejiang China. sikanderpal@zju.edu.cn
Sikander Pal
SP
Choudhary
H Volkan
HV
Oral
Renu
R
Bhardwaj
Jing-Quan
JQ
Yu
Lam-Son Phan
LS
Tran
D000040:Abscisic Acid / Q000378:metabolism; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D060406:Brassinosteroids / Q000494:pharmacology*; D003300:Copper / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D006706:Homeostasis; D006861:Hydrogen Peroxide / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D018384:Oxidative Stress; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics; D011073:Polyamines / Q000494:pharmacology*; D031224:Raphanus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D013095:Spermidine / Q000494:pharmacology; D013260:Steroids, Heterocyclic / Q000494:pharmacology; D013312:Stress, Physiological
D000975:Antioxidants; D060406:Brassinosteroids; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011073:Polyamines; D013260:Steroids, Heterocyclic; C030737:indoleacetic acid; D000040:Abscisic Acid; D003300:Copper; D006861:Hydrogen Peroxide; D013095:Spermidine; C023623:brassinolide