Role of SEPALLATA3 (SEP3) as a downstream gene of miR156-SPL3-FT circuitry in ambient temperature-responsive flowering.
SEPALLATA3 (SEP3) is important in determining flowering time as well as floral organ identity. Although much is known about the regulation of floral organ identity by SEP3, its role as a downstream gene of FLOWERING LOCUS T (FT) for the regulation of ambient temperature-responsive flowering is poorly understood. Here, we show that SEP3 as a downstream gene of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE3 (SPL3) and FT modulates the flowering time in response to different ambient temperatures. SEP3 overexpression showed temperature-insensitive flowering at 23°C and 16°C. This suggests that altered SEP3 activity affects ambient temperature-responsive flowering. However, a lesion in SEP3 did not obviously affect ambient temperature-responsive flowering. SEP3 expression was affected by altered SPL3 and FT activities in the leaf and shoot apical regions at different temperatures. These results suggest that the miR156-SPL3-FT circuitry directly or indirectly regulates SEP3 expression for the regulation of ambient temperature-responsive flowering in Arabidopsis.
Creative Research Initiatives, Division of Life Sciences, Korea University, Seoul, South Korea.
Jeong
J
Hwan Lee
Jae
J
Joon Kim
Ji Hoon
JH
Ahn
D017360:Arabidopsis / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D035264:Flowers / Q000254:growth & development*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D018519:Meristem / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C418905:FT protein, Arabidopsis; D018398:Homeodomain Proteins; D035683:MicroRNAs; C559759:Mirn156 microRNA, Arabidopsis; C121158:SEP3 protein, Arabidopsis; C108465:SPL3 protein, Arabidopsis; D014157:Transcription Factors