[Dependence of the anticoagulant activity of starch and inulin on their degree of sulfonation].
We have studied a relationship between the degree of sulfonation and anticoagulant activity of starch from Solanum tuberosum (molecular weight, 25000-30000 Da; sulfonation degree, 0.4-2.5) and inulin from Helianthus tuberosus (molecular weight, 7000-8000 Da; sulfonation degree, 0.6-1.6). Starch and inulin sulfates (i) increased the time of appearance of fibrin clots in plasma in coagulometric tests and (ii) reduced (via antithrombin) the rate of thrombin-induced hydrolysis of a chromogen substrate. The antithrombin (aIIa) activity of starch sulfates reached 16.8-70.0 IU/mg and the activity against factor Xa (aXa activity) was 2.3-16.6 IU/mg. The antithrombin activity of inulin sulfates was within 5.5-11.4 IU/mg and the activity against factor Xa (aXa activity) was within 0-1.4 IU/mg. An increase in the degree of sulfonation led to a growth in the anticoagulant activity of starch sulfates. The anticoagulant activity of starch sulfates and inulin sulfate with sulfonation degree 1.0 is mediated by antithrombin, which is the plasma inhibitor of serine proteases.
N N
NN
Drozd
M A
MA
Torlopov
A A
AA
Kuzhim
V A
VA
Makarov
D000925:Anticoagulants / Q000494:pharmacology*; D000990:Antithrombin III / Q000037:antagonists & inhibitors; D001777:Blood Coagulation / Q000187:drug effects*; D065427:Factor Xa Inhibitors; D005337:Fibrin / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D006368:Helianthus / Q000737:chemistry; D006801:Humans; D007444:Inulin / Q000031:analogs & derivatives / Q000494:pharmacology*; D008970:Molecular Weight; D011198:Solanum tuberosum / Q000737:chemistry; D013213:Starch / Q000031:analogs & derivatives / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013457:Sulfur Compounds / Q000494:pharmacology*; D013917:Thrombin / Q000037:antagonists & inhibitors
D000925:Anticoagulants; D065427:Factor Xa Inhibitors; D013457:Sulfur Compounds; D000990:Antithrombin III; D005337:Fibrin; D013213:Starch; D007444:Inulin; D013917:Thrombin