Processing of high resolution magic angle spinning spectra of breast cancer cells by the filter diagonalization method.
Proton nuclear magnetic resonance ((1)H NMR) spectroscopy for detection of biochemical changes in biological samples is a successful technique. However, the achieved NMR resolution is not sufficiently high when the analysis is performed with intact cells. To improve spectral resolution, high resolution magic angle spinning (HR-MAS) is used and the broad signals are separated by a T(2) filter based on the CPMG pulse sequence. Additionally, HR-MAS experiments with a T(2) filter are preceded by a water suppression procedure. The goal of this work is to demonstrate that the experimental procedures of water suppression and T(2) or diffusing filters are unnecessary steps when the filter diagonalization method (FDM) is used to process the time domain HR-MAS signals. Manipulation of the FDM results, represented as a tabular list of peak positions, widths, amplitudes and phases, allows the removal of water signals without the disturbing overlapping or nearby signals. Additionally, the FDM can also be used for phase correction and noise suppression, and to discriminate between sharp and broad lines. Results demonstrate the applicability of the FDM post-acquisition processing to obtain high quality HR-MAS spectra of heterogeneous biological materials.
Instituto de Química de São Carlos, Universidade de São Paulo, Av. Trabalhador São-carlense 400, São Carlos, SP, Brazil 13560-970.
Roberta Manzano
RM
Maria
Tiago Bueno
TB
Moraes
Claudio José
CJ
Magon
Tiago
T
Venâncio
Wanessa Fernanda
WF
Altei
Adriano Defini
AD
Andricopulo
Luiz Alberto
LA
Colnago
D000465:Algorithms; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D019906:Nuclear Magnetic Resonance, Biomolecular; D014867:Water / Q000737:chemistry
D014867:Water