Preparation and characterization of poly(L-histidine)/poly(L-glutamic acid) multilayer on silicon with nanometer-sized surface structures.
The specific design and modification of surfaces is of great interest, especially for functional surfaces and medical applications. In order to obtain films on a surface, the layer-by-layer deposition of polyelectrolytes represents a well-established methodology. The alternating deposition of poly(L-histidine) and poly(L-glutamic acid) results in a defined, continuous surface coating that was thoroughly characterized using X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy, ellipsometry, X-ray reflectometry, atomic force microscopy, scanning electron microscopy, contact angle, and electrokinetic measurements. Surface charge, film growth, and final thickness were measured and cross-validated. Additionally, the chemical composition and distribution of polyelectrolytes in the layerstack were determined. Finally, the optical parameters were specified and the surface topography was visualized by several methods. These characterizations revealed a coating with embedded spheroids forming from the bottom layers. This rough surface formed by (PLH/PGA)(8) was highly reproducible and might provide unique features for the design of tailored surfaces.
Institute for Bioprocessing and Analytical Measurement Techniques, Rosenhof, 37308 Heilbad Heiligenstadt, Germany. Xenia.Vinzenz@iba-heiligenstadt.de
Xenia
X
Vinzenz
Erwin
E
Hüger
Marcel
M
Himmerlich
Stefan
S
Krischok
Sebastian
S
Busch
Jürgen
J
Wöllenstein
Christian
C
Hoffmann
D018698:Glutamic Acid / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D056951:Photoelectron Spectroscopy; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
C033223:polyhistidine; D018698:Glutamic Acid; D006639:Histidine; D012825:Silicon