Profiling of miRNAs in pediatric asthma: upregulation of miRNA-221 and miRNA-485-3p.
The aim of this study was to investigate the expression profiles of microRNAs             (miRNAs) in pediatric asthma and to determine candidate miRNAs responsible for             the pathogenesis of this disease. Microarrays were used to detect the differences             in the miRNA expression levels between asthmatic children and controls. Airway             inflammation was evaluated by cell counting and tissue biopsy in an ovalbumin             (OVA)-induced murine asthma model. Real-time polymerase chain reaction (PCR) was             used to verify the differentially expressed miRNAs. The targets of the identified             miRNAs were analyzed by bioinformatic analysis. The sprouty-related protein with             an EVH1 domain-2 (Spred-2) protein content was assessed by western blotting. Differences             were observed in the expression of miRNAs between the asthmatic children and controls.             Upregulation of miRNA-221 and miRNA-485-3p in pediatric asthmatics and murine             asthma models were verified by real-time PCR. Spred-2, a predicted target of miRNA-221             and miRNA-485-3p, was downregulated in murine asthma models. Upregulation of miRNA-221             and miRNA-485-3p may regulate the pathogenesis of asthma.
Department of Respiratory Medicine, Nanjing Children's Hospital Affiliated to Nanjing Medical University, Nanjing 210029, PR China.
Feng
F
Liu
Hou-Bing
HB
Qin
Bing
B
Xu
Hui
H
Zhou
De-Yu
DY
Zhao
D000818:Animals; D001249:Asthma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D019295:Computational Biology; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D010047:Ovalbumin / Q000276:immunology; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
D035683:MicroRNAs; D010047:Ovalbumin; D012097:Repressor Proteins; C525709:MIR221, human; C556501:MIRN485 microRNA, human; C500760:SPRED2 protein, human