Cytosolic Ca(2+) determinations in studying plant responses to salinity and oxidative stress.
Salinity and oxidative stress can transiently elevate cytosolic free Ca(2+) ([Ca(2+)](cyt)) of plant cells. The [Ca(2+)](cyt) increase may be part of a signaling cascade or cell death, depending on cell type, the magnitude and the duration of stress exposure. Several approaches for determining [Ca(2+)](cyt) responses are available to plant biologists, but some require highly specialized equipment. Here we describe protocols for using aequorin as a standard [Ca(2+)](cyt) reporter, with output detected with a plate-reader luminometer.
Department of Plant Sciences, University of Cambridge, Cambridge, UK.
Anuphon
A
Laohavisit
Renato
R
Colaço
Julia
J
Davies
D000331:Aequorin / Q000378:metabolism; D001059:Apoproteins / Q000378:metabolism; D017360:Arabidopsis / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D018384:Oxidative Stress; D059828:Plant Cells / Q000378:metabolism*; D018517:Plant Roots / Q000502:physiology; D011523:Protoplasts / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D054712:Salinity
D001059:Apoproteins; D011994:Recombinant Proteins; C060014:apoaequorin; D000331:Aequorin; D002118:Calcium