Detection of bond formations by DNA-programmed chemical reactions and PCR amplification.
A system capable of performing both DNA-templated chemical reactions and detection of bond formations is reported. Photocleavable DNA templates direct reactions. Products from bond-forming events re-ligate original templates, amplifiable by PCR, therefore distinguishing bond formation from background. This system provides a novel approach for discovering potential new chemical reactions.
Key Laboratory of Bioorganic Chemistry and Molecular Engineering of the Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 202 Chengfu Rd., Beijing, 100871, China.
Yizhou
Y
Li
Mingda
M
Zhang
Chi
C
Zhang
Xiaoyu
X
Li
D004247:DNA / Q000737:chemistry*; D010782:Photolysis; D011092:Polyethylene Glycols / Q000737:chemistry; D016133:Polymerase Chain Reaction
D011092:Polyethylene Glycols; D004247:DNA