Role of smart polymers in protein purification and refolding.
Affinity precipitation is a non-chromatographic method which is useful for purification and refolding of proteins. Quite often, a stimuli-sensitive polymer can be identified which selectively binds to the desired protein. For separation, the protein can be recovered from the precipitate of the protein-smart polymer complex. In case of a refolding experiment, binding of the solubilized protein (in its denatured form) with the polymer leads to the refolding of the protein.
Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India.
Saurabh
S
Gautam
Priyanka
P
Dubey
Raghavan
R
Varadarajan
Munishwar N
MN
Gupta
D000464:Alginates / Q000737:chemistry; D011232:Chemical Precipitation; D048271:Chitosan / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D011109:Polymethacrylic Acids / Q000737:chemistry; D011489:Protein Denaturation; D058849:Protein Refolding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D000464:Alginates; D006603:Hexuronic Acids; D011109:Polymethacrylic Acids; D011506:Proteins; C038300:methylmethacrylate-methacrylic acid copolymer; D020723:Glucuronic Acid; D048271:Chitosan