[Involvement of p38 MAPK pathway in GLP-1-induced inhibition of apoptosis in human umbilical vein endothelial cells].
The aim of the present study was to investigate the effect of glucagon-like peptide-1 (GLP-1) on palmitate-induced apoptosis of human umbilical vein endothelial cells (HUVECs) and the underlying mechanism. HUVECs were cultured in vitro, and then treated by palmitate to induce apoptosis. Meanwhile, GLP-1 was added to explore its effect. After 24 h of the treatments, Caspase-3 activity and DNA fragmentation were measured using ELISA kits. Phospho-p38 mitogen-activated protein kinase (p-p38 MAPK) expression was detected by Western blot. The results showed that incubating HUVECs with 0.125 mmol/L GLP-1 increased Caspase-3 activity and DNA fragmentation. GLP-1 significantly inhibited palmitate-induced increases of Caspase-3 activity and DNA fragmentation in a concentration-dependent manner. Moreover, GLP-1 inhibited the up-regulation of p-p38 MAPK expression induced by palmitate in HUVECs. These results suggest GLP-1 protects HUVECs against lipo-apoptosis, and this effect may be mediated through inhibiting p38 MAPK pathway.
Department of Pathophysiology, Ningxia Medical University, Yinchuan 750004, China.
Hua
H
Xu
Hai-Long
HL
Li
Zi-Yong
ZY
Niu
Gui-Zhong
GZ
Li
Jun
J
Cao
Yi-Deng
YD
Jiang
D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D053938:DNA Fragmentation; D052216:Glucagon-Like Peptide 1 / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D020935:MAP Kinase Signaling System; D015854:Up-Regulation; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D052216:Glucagon-Like Peptide 1; D048051:p38 Mitogen-Activated Protein Kinases; C505407:CASP3 protein, human; D053148:Caspase 3