Geno-tox: cell array biochip for genotoxicity monitoring and classification.
In vitro genotoxicity tests detect carcinogens that are thought to act primarily via a mechanism involving direct genetic damage. In this study, we constructed a Geno-Tox cell array chip for genotoxicity testing; eight recombinant bioluminescent bacteria were used to successfully fabricate a Geno-Tox cell array chip. Four well-characterized DNA damage chemicals were selected to determine the capabilities of this Geno-Tox array chip, and each strain of the chip was distinctly responsive, according to the specific mode of genotoxic action. Therefore, this Geno-Tox cell array chip could be implemented to characterize and understand the genotoxic modes of impact; thus, it could be used to provide the genotoxic mechanism of action for new drugs or unknown or newly synthesized chemicals in food and the environment.
MD Anderson Cancer Center, Department of Experimental Radiation Oncology, University of Texas, 1515 Holcombe Blvd., Houston, TX 77030, USA.
Joo-Myung
JM
Ahn
Man Bock
MB
Gu
D001419:Bacteria / Q000166:cytology* / Q000187:drug effects / Q000235:genetics*; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects; D004249:DNA Damage; D004274:DNA, Recombinant / Q000235:genetics; D005818:Genetic Engineering; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity; D011401:Promoter Regions, Genetic / Q000235:genetics; D046888:Tissue Array Analysis / Q000379:methods*
D004274:DNA, Recombinant; D009153:Mutagens