Molecular functions of chaperonin gene, containing tailless complex polypeptide 1 from Macrobrachium rosenbergii.
Chaperonin (MrChap) was identified from a constructed transcriptome dataset of freshwater prawn Macrobrachium rosenbergii. The MrChap peptide contains a long chaperone super family domain between 11 and 525. Three chaperone tailless complex polypeptide (TCP-1) signatures are present in the MrChap peptide sequence at 36-48, 57-73 and 85-93. The gene expressions of MrChap in both healthy M. rosenbergii and those infected with infectious hypodermal and hematopoietic necrosis virus (IHHNV) were examined using qRT-PCR. To understand its biological activity, the recombinant MrChap gene was constructed and expressed in Escherichia coli BL21 (DE3). The results of ATPase assay showed that the recombinant MrChap protein exhibited apparent ATPase activity. Chaperone activity assay showed that the recombinant MrChap protein is an active chaperone. These results suggest that MrChap is potentially involved in the immune responses against viral infection in M. rosenbergii. These findings indicate that the recombinant MrChap protein may be used in immunotherapeutic approaches.
Department of Biotechnology, Faculty of Science and Humanities, SRM University, SRM Nagar, Kattankulathur 603 203, Chennai, Tamil Nadu, India. jesuaraj@gmail.com
Jesu
J
Arockiaraj
Puganeshwaran
P
Vanaraja
Sarasvathi
S
Easwvaran
Arun
A
Singh
Rofina Yasmin
RY
Othman
Subha
S
Bhassu
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D018833:Chaperonins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D019223:Densovirinae / Q000502:physiology*; D008969:Molecular Sequence Data; D033541:Palaemonidae / Q000235:genetics* / Q000378:metabolism / Q000821:virology*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D011994:Recombinant Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D018833:Chaperonins