Frequency of loss of heterozygosity of the NF2 gene in schwannomas from Croatian patients.
OBJECTIVE
To identify gross deletions in the NF2 gene in a panel of schwannomas from Croatian patients in order to establish their frequencies in Croatian population.


METHODS
Changes of the NF2 gene were tested by polymerase chain reaction/loss of heterozygosity (LOH) using two microsatellite markers, D22S444 and D22S929.


RESULTS
The analysis with both markers demonstrated that 43.75% of schwannomas exhibited LOH of the NF2 gene. The D22S444 region exhibited 45.5% of LOHs and the D22S929 region exhibited 14.3% of LOHs. Four LOHs were found in Antoni B, 2 in Antoni A, and 1 in Antoni A and B type tumors.


CONCLUSIONS
The frequency of changes observed in Croatian patients is broadly similar to that reported in other populations and thus confirms the existing hypothesis regarding the tumorigenesis of schwannomas and contributes to schwannoma genetic profile helping us to better understand its etiology and treatment.
Laboratory of Neurooncology, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Salata 12, HR-10000 Zagreb, Croatia. nina@mef.hr
Nives
N
Pećina-Slaus
Martina
M
Zeljko
Hrvoje Ivan
HI
Pećina
Tamara
T
Nikuseva Martić
Niko
N
Bacić
Davor
D
Tomas
Reno
R
Hrasćan
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D017523:Croatia; D005260:Female; D005787:Gene Frequency; D016515:Genes, Neurofibromatosis 2; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D009423:Nervous System Neoplasms / Q000235:genetics* / Q000473:pathology; D009442:Neurilemmoma / Q000235:genetics* / Q000473:pathology; D055815:Young Adult