Successful delivery derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia.
OBJECTIVE
To report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with vitrified sperm from patients with severe male factor infertility.


METHODS
Retrospective case series.


METHODS
IVF unit of a medical center.


METHODS
Three patients with severe oligozoospermia or nonobstructive azoospermia (NOA).


METHODS
Cryopreservation of limited numbers of spermatozoa with the use of Cryotop and Cell Sleeper as nonbiologic containers.


METHODS
Four cycles underwent intracytoplasmic sperm injection (ICSI) with vitrified sperm.


RESULTS
A total of 148 spermatozoa in 18 containers (8.2 sperm per container) were vitrified and 36 of them (5 containers) were warmed. Thirty-three sperm (92%) were retrieved successfully and injected individually into 17 mature oocytes. Fertilization was observed in 12 oocytes (71%), and all zygotes (100%) cleaved. A couple with NOA achieved a singleton pregnancy and concluded with full-term delivery of a healthy boy (2,632 g).


CONCLUSIONS
A successful delivery was achieved after transfer of a blastocyst derived from vitrified-warmed spermatozoa. A small number of vitrified sperm cells were used for ICSI to fertilize oocytes with predictable timing.
IVF Center, Kurashiki Medical Clinic, Kurashiki, Japan. ivf@fkmc.or.jp
Yuji
Y
Endo
Yoshitaka
Y
Fujii
Shozo
S
Kurotsuchi
Hiroaki
H
Motoyama
Hiroaki
H
Funahashi
D000328:Adult; D053713:Azoospermia / Q000628:therapy*; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006801:Humans; D050498:Live Birth; D008297:Male; D011247:Pregnancy; D012662:Semen Preservation / Q000379:methods*; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D016896:Treatment Outcome