Antibiotic resistance and substrate profiles of the class A carbapenemase KPC-6.
The class A carbapenemase KPC-6 produces resistance to a broad range of β-lactam antibiotics. This enzyme hydrolyzes penicillins, the monobactam aztreonam, and carbapenems with similar catalytic efficiencies, ranging from 10(5) to 10(6) M(-1) s(-1). The catalytic efficiencies of KPC-6 against cephems vary to a greater extent, ranging from 10(3) M(-1) s(-1) for the cephamycin cefoxitin and the extended-spectrum cephalosporin ceftazidime to 10(5) to 10(6) M(-1) s(-1) for the narrow-spectrum and some of the extended-spectrum cephalosporins.
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA.
Toni L
TL
Lamoureaux
Hilary
H
Frase
Nuno T
NT
Antunes
Sergei B
SB
Vakulenko
D001398:Aztreonam / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D055162:Biocatalysis; D015780:Carbapenems / Q000378:metabolism* / Q000494:pharmacology; D002511:Cephalosporins / Q000378:metabolism* / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006868:Hydrolysis; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D010406:Penicillins / Q000378:metabolism* / Q000494:pharmacology; D013379:Substrate Specificity; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D015780:Carbapenems; D002511:Cephalosporins; D007527:Isoenzymes; D010406:Penicillins; D001618:beta-Lactamases; C063912:carbapenemase; D001398:Aztreonam