Effect of Etiasa on the expression of matrix metalloproteinase-2 and             tumor necrosis factor-α in a rat model of ulcerative colitis.
The aim of this study was to determine the disease activity index (DAI)             and the colonic mucosa damage index (CMDI), and to detect the colonic mucosal             expression levels of matrix metalloproteinase-2 (MMP-2) and tumor necrosis factor-α             (TNF-α) in rats with ulcerative colitis (UC). We also aimed to investigate the             protective role of Etiasa in UC. Sprague Dawley (SD) rats were randomly divided             into three groups: the control, an Etiasa-treated group and a UC model group.             Rats were sacrificed on days 14, 21, 35 or 56 following the administration of             treatment by enema and the DAI, CMDI and colonic expression levels of MMP-2 and             TNF-α were assessed. In the UC model group, the DAI and CDMI scores and the colonic             expression levels of MMP-2 and TNF-α increased significantly compared with the             control at all timepoints, and were also significantly higher than those in the             Etiasa-treated group. In conclusion, the expression levels of MMP-2 and TNF-α             increased significantly in rats with UC. Etiasa reduces colonic mucosal damage             by downregulating the expression of MMP-2 and TNF-α.
Department of Gastroenterology, The First Afﬁliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, PR China.
Jing-Wei
JW
Mao
Hai-Ying
HY
Tang
Xiao-Yan
XY
Tan
Ying-De
YD
Wang
D000636:Aminosalicylic Acids / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000187:drug effects; D004334:Drug Administration Schedule; D004733:Enema; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000636:Aminosalicylic Acids; D014409:Tumor Necrosis Factor-alpha; D020778:Matrix Metalloproteinase 2