Selection and use of intracellular antibodies (intrabodies).
Intracellularly expressed recombinant antibodies, or intrabodies, are powerful tools for cell biology studies as well as therapeutic applications. Cell biologists use them to either block the intracellular antibody target or to image endogenous target dynamics. We describe here methods to select recombinant antibodies from antibody phage display libraries and to subsequently express them as fluorescent intrabodies.
Institut Curie, CNRS UMR144, Paris, France.
Sandrine
S
Moutel
Clément
C
Nizak
Franck
F
Perez
D000906:Antibodies / Q000378:metabolism*; D061505:Cell Surface Display Techniques / Q000379:methods*; D005455:Fluorescent Antibody Technique; D005822:Genetic Vectors / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D042541:Intracellular Space / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection
D000906:Antibodies; D011993:Recombinant Fusion Proteins