Detection of phosphorylation states by intermolecular sensitization of lanthanide-peptide conjugates.
The luminescence of a designed peptide equipped with a coordinatively-unsaturated lanthanide complex is modulated by the phosphorylation state of a serine residue in the sequence. While the phosphorylated state is weakly emissive, even in the presence of an external antenna, removal of the phosphate allows coordination of the sensitizer to the metal, yielding a highly emissive supramolecular complex.
Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares, Departamento de Química Orgánica and Unidad Asociada al CSIC, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Elena
E
Pazos
Marko
M
Goličnik
José L
JL
Mascareñas
M Eugenio
ME
Vázquez
D056831:Coordination Complexes / Q000737:chemistry*; D005063:Europium / Q000737:chemistry; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D010748:Phosphopeptides / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000737:chemistry
D056831:Coordination Complexes; D028581:Lanthanoid Series Elements; D010748:Phosphopeptides; D013725:Terbium; D005063:Europium; D012694:Serine