Effect of β-(3,4-dihydroxyphenyl)lactic acid on oxidative stress stimulated by high glucose levels in human peritoneal mesothelial cells.
OBJECTIVE
To investigate the effects of β-(3,4-dihydroxyphenyl)lactic acid on oxidative stress stimulated by high glucose levels in human peritoneal mesothelial cells (HPMCs) in vitro.


METHODS
HPMCs were incubated with 100 mol/l glucose followed by 0.625-20 mg/ml β-(3,4-dihydroxyphenyl)lactic acid. Reactive oxygen species (ROS) were quantified by flow cytometry. Relative levels of fibronectin-1 (FN1), collagen-I α(1) (COL1A1), endothelin-1 (EDN1) and haem oxygenase-1 (HMOX1) mRNA and protein were quantified by real-time reverse transcription-polymerase chain reaction and Western blotting, respectively. Absolute levels of FN1 and COLIA1 were quantified by enzyme-linked immunosorbent assay.


RESULTS
β-(3,4-Dihydroxyphenyl)lactic acid significantly decreased ROS levels, and EDN1 mRNA and protein levels, in dose- and time-dependent manners. HMOX1 mRNA and protein levels were significantly increased by β-(3,4-dihydroxyphenyl)lactic acid in dose-dependent manners. COL1A1 and FN1 mRNA and protein levels were significantly decreased by β-(3,4-dihydroxyphenyl)lactic acid in dose- and time-dependent manners.


CONCLUSIONS
β-(3,4-Dihydroxyphenyl)lactic acid inhibited oxidative stress and reversed increases in FN1 and COLIA1 induced by high glucose levels in HPMCs. This may contribute to a protective role in peritoneal fibrosis.
Department of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
H
H
Zhang
J-W
JW
Wang
Y
Y
Xu
K
K
Zhang
B
B
Yi
J
J
Sun
Y
Y
Liu
X-M
XM
Zhang
J-S
JS
Liu
D001483:Base Sequence; D015153:Blotting, Western; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D019332:Endothelin-1 / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism; D005434:Flow Cytometry; D005947:Glucose / Q000378:metabolism*; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007773:Lactates / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D010537:Peritoneum / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
D024042:Collagen Type I; D017931:DNA Primers; D019332:Endothelin-1; D005353:Fibronectins; D007773:Lactates; D017382:Reactive Oxygen Species; C035055:3,4-dihydroxyphenyllactic acid; D051547:Heme Oxygenase-1; D005947:Glucose