Extracellular matrix metalloproteinase inducer is associated with severity of brain oedema following experimental subarachnoid haemorrhage in rats.
OBJECTIVE
Brain oedema is a major cause of clinical deterioration and death following brain trauma; cellular mechanisms involved in its development remain elusive. This study investigated the role of extracellular matrix metalloproteinase inducer (EMMPRIN) in brain oedema.


METHODS
The monofilament puncture model was used to induce subarachnoid haemorrhage. Adult male Sprague-Dawley rats were divided into five groups (n = 20 per group): sham-operated, sacrificed immediately after surgery (sham group); sacrificed 12, 24 or 72 h after subarachnoid haemorrhage  induction (SAH-12, SAH-24 and SAH-72 groups, respectively); treated with EMMPRIN inhibitor immediately after subarachnoid haemorrhage, sacrificed at 24 h (SAH-inhibition group). Mean brain water content, and EMMPRIN mRNA and protein levels, were determined.


RESULTS
Compared with the sham  group, mean brain water content, EMMPRIN mRNA and protein levels in the SAH-12, SAH-24 and SAH-72 groups increased rapidly and significantly (maximal at 24 h). EMMPRIN inhibition significantly reduced mean brain water content and EMMPRIN mRNA and protein levels in the SAH-inhibition group,  compared with the SAH-24 group.


CONCLUSIONS
EMMPRIN upregulation may be important in the formation of brain oedema; inhibition of EMMPRIN activity may provide a potential approach to reduce oedema after subarachnoid haemorrhage.
Experimental Surgery Department, Tangdu Hospital, The Fourth Military Medical University, Xi'an, China.
Y
Y
Tu
J
J
Fu
J
J
Wang
G
G
Fu
L
L
Wang
Y
Y
Zhang
D000818:Animals; D001483:Base Sequence; D051926:Basigin / Q000235:genetics / Q000502:physiology*; D015153:Blotting, Western; D001929:Brain Edema / Q000503:physiopathology*; D017931:DNA Primers; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index; D013345:Subarachnoid Hemorrhage / Q000503:physiopathology*
D017931:DNA Primers; D012333:RNA, Messenger; D051926:Basigin