Transcriptome analysis of membrane transporters in response to salinity stress.
Exposure to high ambient levels of NaCl affects plant water relations and creates ionic stress. To a large extent, responses to such stress depend on the action of membrane transporters, particularly those that move cations such as Na(+) and K(+). A genomics approach can greatly help with the identification of important membrane transporter genes. This can be done by comparing transcriptomes of salinized and non-salinized plants, by comparing tolerant and non-tolerant species, or by using intraspecies variation. This chapter describes a protocol using oligo-microarrays to compare salinity treated (50 mM NaCl) and non-treated rice roots, presenting protocols for growth, RNA isolation, cDNA synthesis and labeling, and a summary of data collection, analysis, and interpretation. Although focused on rice root tissue, the described procedures can be applied to many different treatments, tissues, and plant species.
Department of Botany, The Open University of Sri Lanka, Nugegoda, Sri Lanka.
Prasad
P
Senadheera
Frans J M
FJ
Maathuis
D020869:Gene Expression Profiling / Q000379:methods*; D026901:Membrane Transport Proteins / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D018517:Plant Roots / Q000235:genetics*; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics; D013312:Stress, Physiological; D059467:Transcriptome
D026901:Membrane Transport Proteins