Cutting edge: intravascular staining redefines lung CD8 T cell responses.
Nonlymphoid T cell populations control local infections and contribute to inflammatory diseases, thus driving efforts to understand the regulation of their migration, differentiation, and maintenance. Numerous observations indicate that T cell trafficking and differentiation within the lung are starkly different from what has been described in most nonlymphoid tissues, including intestine and skin. After systemic infection, we found that >95% of memory CD8 T cells isolated from mouse lung via standard methods were actually confined to the pulmonary vasculature, despite perfusion. A respiratory route of challenge increased virus-specific T cell localization within lung tissue, although only transiently. Removing blood-borne cells from analysis by the simple technique of intravascular staining revealed distinct phenotypic signatures and chemokine-dependent trafficking restricted to Ag-experienced T cells. These results precipitate a revised model for pulmonary T cell trafficking and differentiation and a re-evaluation of studies examining the contributions of pulmonary T cells to protection and disease.
Department of Microbiology, Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA.
Kristin G
KG
Anderson
Heungsup
H
Sung
Cara N
CN
Skon
Leo
L
Lefrancois
Angela
A
Deisinger
Vaiva
V
Vezys
David
D
Masopust
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D016827:CD8 Antigens / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology* / Q000637:transplantation; D002196:Capillaries / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D008168:Lung / Q000098:blood supply* / Q000276:immunology* / Q000378:metabolism; D008216:Lymphocytic Choriomeningitis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D037342:Pertussis Toxin / Q000008:administration & dosage; D011024:Pneumonia, Viral / Q000235:genetics / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000276:immunology / Q000473:pathology / Q000637:transplantation; D013194:Staining and Labeling / Q000379:methods
D000906:Antibodies; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; C526846:Cd8b1 protein, mouse; D037342:Pertussis Toxin