[Molecular epideiological and clinical feature of human calicivirus and adenovirus among children with diarrhea less than 5 years old from 2010 to 2011 in Lanzhou, Gansu province].
OBJECTIVE
To investigate the clinical and molecular epidemiology characteristics of calicivirus and adenovirus in children for viral diarrhea in Lanzhou.


METHODS
Stool samples were collected from 295 children with diarrhea at the First Hospital of Lanzhou University, Gansu Province,China, between July 2010 and June 2011. Reverse transcription-polymerase chain reaction (RT-PCR) or PCR were used to detected calicivirus and adenovirus. The adenovirus positive samples were typed by nested PCR and multiple PCR.


RESULTS
Of the 295 specimens, 13.2% (39/295) were positive for calicivirus, and 5.1% (15/295) were adenovirus. Typing and Phylogenetic analysis revealed that novirus GII-3 and adenovirus 41 were the dominant strains. Both calicivirus and adenovirus predominately affect children under the age of 2. In seasonal distribution, there was no obvious peak.


CONCLUSIONS
Human calicivirus and adenovirus are important pathogens of viral diarrhea,it is important to develop long-term systematic surveillance.
First Clinical Medical College of Lanzhou University, Gangsu 730000, China.
Yong-Xia
YX
Wang
Dan-Di
DD
Li
Yu
Y
Jin
Qing
Q
Zhang
Hong
H
Wang
Xiang-Yu
XY
Kong
Yu-Ning
YN
Li
Zhao-Jun
ZJ
Duan
D000257:Adenoviridae Infections / Q000453:epidemiology* / Q000821:virology; D000260:Adenoviruses, Human / Q000235:genetics*; D002139:Caliciviridae / Q000235:genetics*; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D017720:Molecular Epidemiology; D013997:Time Factors