Dehalogenimonas alkenigignens sp. nov., a chlorinated-alkane-dehalogenating bacterium isolated from groundwater.
Two strictly anaerobic bacterial strains, designated IP3-3(T) and SBP-1, were isolated from groundwater contaminated by chlorinated alkanes and alkenes at a Superfund Site located near Baton Rouge, Louisiana (USA). Both strains reductively dehalogenate a variety of polychlorinated aliphatic alkanes, including 1,2-dichloroethane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane and 1,2,3-trichloropropane, when provided with hydrogen as the electron donor. To clarify their taxonomic position, strains IP3-3(T) and SBP-1 were characterized using a polyphasic approach. Both IP3-3(T) and SBP-1 are mesophilic, non-spore-forming, non-motile and Gram-stain-negative. Cells of both strains are irregular cocci with diameters of 0.4-1.1 µm. Both are resistant to ampicillin and vancomycin. The genomic DNA G+C contents of strains IP3-3(T) and SBP-1 are 55.5±0.4 and 56.2±0.2 mol% (HPLC), respectively. Major cellular fatty acids include C18 : 1ω9c, C16 : 0, C14 : 0 and C16 : 1ω9c. 16S rRNA gene sequence based phylogenetic analyses indicated that the strains cluster within the phylum Chloroflexi most closely related to but distinct from the species Dehalogenimonas lykanthroporepellens (96.2 % pairwise similarity) and Dehalococcoides mccartyi (90.6 % pairwise similarity). Physiological and chemotaxonomic traits as well as phylogenetic analysis support the conclusion that these strains represent a novel species within the genus Dehalogenimonas for which the name Dehalogenimonas alkenigignens sp. nov. is proposed. The type strain is IP3-3(T) ( = JCM 17062(T) = NRRL B-59545(T)).
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.|Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
Kimberly S
KS
Bowman
Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal.
M Fernanda
MF
Nobre
Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal.
Milton S
MS
da Costa
Department of Biological Sciences, University of Alaska Anchorage, Anchorage, Alaska 99508, USA.|Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
Fred A
FA
Rainey
Department of Civil and Environmental Engineering, Louisiana State University, Baton Rouge, LA 70803, USA.
William M
WM
Moe
D000473:Alkanes / Q000378:metabolism*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D041862:Chloroflexi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D060587:Groundwater / Q000382:microbiology*; D006843:Hydrocarbons, Chlorinated / Q000378:metabolism*; D008145:Louisiana; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D000473:Alkanes; D004269:DNA, Bacterial; D006843:Hydrocarbons, Chlorinated; D012336:RNA, Ribosomal, 16S