[Genotyping and variability of HIV-1 in 26 cases of paid blood donors].
OBJECTIVE
To analyze the genome mutations of HIV-1 gag, pol and env genes from HIV-infected paid blood donors in rural central China.


METHODS
DNA was extracted from peripheral blood mononuclear cells, gag (p17-p24), pol (PR-RT), env (C2-V5) genes were amplified by nested polymerase chain reaction (PCR), purified products were sequenced, and sequence data was analyzed by MEGA5.0 soft wares.


RESULTS
Twenty-three samples were subtype B, two samples were recombinant of subtype B and subtype C, one sample was recombinant of subtype CRF01_AE and subtype B. PI major resistance mutations were not found in the PR region. M184V, K101E and G190A were detected in the RT region, respectively.


CONCLUSIONS
Subtype B was the major HIV circulating genetic forms in this area. Most strains were sensitive to high active anti-retroviral therapy (HARRT). 91.7% V3 loop tip motifs of X4-tropic strains was GPGR. It showed that GPGR might be associated with accelerate disease progression to AIDS.
State Key Laboratory for Infectious Disease Control and Prevention, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Fei-Fei
FF
Guo
Guo-Min
GM
Chen
Yi
Y
Zeng
D001782:Blood Donors; D024882:Drug Resistance, Viral / Q000235:genetics; D015751:Genes, pol; D005838:Genotype; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D010802:Phylogeny