Mitochondria of trained skeletal muscle are protected from deleterious effects of statins.
BACKGROUND
Statins are associated with adverse skeletal muscle effects. Our objective was to determine if muscular adaptations following exercise training prevented deleterious effects of atorvastatin in glycolytic skeletal muscle.


METHODS
Twenty rats were divided into 2 groups: a control group (n = 10; Cont) and a 10 days of training group (n = 10; Training). Using the permeabilized fibers technique, we explored mitochondrial function.


RESULTS
Exercise training increased V(max) and H(2)O(2) production without altering the free radical leak, and mRNA expression of SOD2 and Cox1 were higher in trained muscle. In the Cont group, atorvastatin exposure increased H(2)O(2) production and decreased skeletal muscle V(max). The decreased V(max) effect of atorvastatin was dose dependent. Interestingly, the half-maximal inhibitory concentration (IC(50)) was higher in the Training group. H(2)O(2) production increased in trained muscle after atorvastatin exposure.


CONCLUSIONS
These results suggest that improvements in mitochondrial respiratory and antioxidant capacities following endurance training protected mitochondria against statin exposure.
CHRU of Strasbourg, Physiology and Functional Explorations Department, New Civil Hospital, B.P. 426, 67091 Strasbourg, France.
Jamal
J
Bouitbir
Frédéric
F
Daussin
Anne-Laure
AL
Charles
Laurence
L
Rasseneur
Stéphane
S
Dufour
Ruddy
R
Richard
François
F
Piquard
Bernard
B
Geny
Joffrey
J
Zoll
D000818:Animals; D000069059:Atorvastatin; D006538:Heptanoic Acids / Q000009:adverse effects* / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D008297:Male; D008931:Mitochondria, Muscle / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D010807:Physical Endurance / Q000502:physiology; D011758:Pyrroles / Q000009:adverse effects* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism
D006538:Heptanoic Acids; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D011758:Pyrroles; D017382:Reactive Oxygen Species; D000069059:Atorvastatin; D006861:Hydrogen Peroxide