Purification, characterization and antigenic species-specific reactivity of vitellogenin of rosy barb (Puntius conchonius Hamilton).
Vitellogenin (Vg) was isolated using gel filtration and ion-exchange chromatography from plasma of rosy barb (Puntius conchonius) treated with estrogen (estradiol-17beta). The purified Vg was stained positive for carbohydrate, lipid and phosphorus and was rich in Ala (10.58%), Asp (8.46%), Glu (10.30%), Leu (11.23%), Lys (7.22%) and Val (7.49%). It appeared as a single band of approximately 450 kDa in native PAGE and was reduced to a single band of approximately 167 kDa under SDS-PAGE, suggesting that it is probably composed of three identical polypeptide subunits. Double-immunodiffusion assay showed that the plasma from female rosy barb reacted with the mouse antisera against rosy barb Vg, forming a single immunoprecipitin line, while the plasma from male rosy barb or female zebrafish showed no such reactivity, confirming the existence of the sex- and species-specific reactivity for rosy barb Vg antisera.
Department of Marine Biology, Ocean University of China, Qingdao 266003, PR China.
Xiaodong
X
Shi
Shicui
S
Zhang
Yaning
Y
Sun
Qiuxiang
Q
Pang
M S
MS
Sawant
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D000941:Antigens / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange / Q000379:methods; D003429:Cross Reactions; D003530:Cyprinidae; D004058:Diffusion; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D004967:Estrogens / Q000737:chemistry*; D005260:Female; D005399:Fishes; D007106:Immune Sera / Q000737:chemistry; D008297:Male; D010949:Plasma / Q000737:chemistry; D014819:Vitellogenins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*
D000596:Amino Acids; D000941:Antigens; D002241:Carbohydrates; D004967:Estrogens; D007106:Immune Sera; D014819:Vitellogenins