Cellular localization of the embryo-specific hybrid PRP from Zea mays, and characterization of promoter regulatory elements of its gene.
The expression, regulation and cellular localization of ZmHyPRP, a gene marker of embryo differentiation whose expression declines after ABA induction, was studied. ZmHyPRP is a proline-rich protein with a C-terminal domain having eight cysteines in a CM8 pattern. Transient expression in onion epidermal cells, transformed with a 2x35S::ZmHyPRP-GFP construction, indicated the protein is present in vesicles lining the membrane of the cell. The ZmHyPRP gene expression is under the control of classic promoter seed-specific regulatory elements such as Sph/RY and G-boxes, suggesting regulation by B3 and b-ZIP transcription factors. Promoter deletion analysis, by particle-bombardment transient transformation of maize immature embryos with serial deletions of the promoter fused to GUS, showed the presence of two negative regulatory elements, NE1 (-2070 to -1280) and NE2 (-232 to -178), in the ZmHyPRP promoter. By selective deletion or mutation of ZmHyPRP regulatory promoter elements we conclude that the promoter expression is attenuated by the NE2 element as well as by the G-box2 and the Sph1-2 box together with the G-box2.
Centre de Recerca en Agrigenòmica (CRAG), CSIC-IRTA-UAB-UB, Edifici CRAG, Campus UAB, Bellaterra (Cerdanyola del Vallés), 081993 Barcelona, Spain, matilde.jose@cragenomica.es
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Jose-Estanyol
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Puigdomènech
D000040:Abscisic Acid / Q000494:pharmacology; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D019697:Onions / Q000235:genetics / Q000378:metabolism; D009928:Organ Specificity; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D011993:Recombinant Fusion Proteins; D012639:Seeds / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D003313:Zea mays / Q000187:drug effects / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*
D018744:DNA, Plant; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C076045:hybrid proline-rich protein, plant; D000040:Abscisic Acid