Highly sensitive high-throughput profiling of six phytohormones using MS-probe modification and liquid chromatography-tandem mass spectrometry.
We describe a method for highly sensitive high-throughput analysis of six major phytohormones; cytokinins (23 species), auxins (7 species), abscisic acid (ABA), gibberellins (GAs, 11 species), salicylic acid (SA), and jasmonic acid (JA). The method consists of solid-phase extraction using 96-well column plates and liquid chromatography coupled with a tandem quadrupole mass spectrometer. In order to improve the quantification limit of negatively charged compounds and especially GAs, we use a chemical modification with an "MS-probe" that contains a quaternary amine moiety. The method requires plant tissue of <100 mg fresh weight for quantification and enables us to analyze large number of plant samples at a time.
RIKEN Plant Science Center, Tsurumi, Yokohama, Japan.
Mikiko
M
Kojima
Hitoshi
H
Sakakibara
D000588:Amines / Q000737:chemistry; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D010766:Phosphorylation; D010937:Plant Growth Regulators / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D010944:Plants / Q000737:chemistry; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000588:Amines; D010937:Plant Growth Regulators