Carotenoid-based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production.
Beside their essential cellular functions, isoprenoids have value as pharmaceuticals, nutriceuticals, pesticides, and fuel alternatives. Engineering microorganisms for production of isoprenoids is relatively easy, sustainable, and cost effective in comparison to chemical synthesis or extraction from natural producers. We introduced genes encoding carotenoid biosynthetic enzymes into the haploid yeast deletion collection to identify gene deletions that improved isoprenoid production. Deletions that showed significant improvement in carotenoid production were further screened for production of bisabolene, an isoprenoid alternative to petroleum-derived diesel. Combining those deletions with other mevalonate pathway modifications increased production of bisabolene from 40mg/L to 800mg/L in shake-flask cultures. In a fermentation process, this engineered strain produced 5.2g/L of bisabolene.
Joint BioEnergy Institute, Emeryville, CA 94608, USA.
Bilge
B
Özaydın
Helcio
H
Burd
Taek Soon
TS
Lee
Jay D
JD
Keasling
D002338:Carotenoids / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D060847:Metabolic Engineering / Q000379:methods*; D013045:Species Specificity; D013729:Terpenes / Q000302:isolation & purification / Q000378:metabolism*; D015003:Yeasts / Q000145:classification* / Q000502:physiology*
D013729:Terpenes; D002338:Carotenoids