Prokaryotic expression, purification, and production of glutathione S-transferase-tagged neural stem cell specific peptides from phage display screening.
Combining stem cell with phage display to discover novel biomarkers is a new field in stem cell studies. Novel peptides obtained through phage display panning have been functionally identified and involved into initial applications as cell culture support or cell label. In the present study, we designed a glutathione S-transferase (GST)-tagged peptide complex to construct an easy and efficient prokaryotic expression and purification platform for peptides obtained from phage display panning. The purified GST-peptide protein could specifically bind to neural stem (NS) cells and could be flexibly used for GST pull down assay or NS cell labeling in future studies. The results of the present study would be useful for cell labeling and future investigations of special receptors on stem cell surface.
Department of Biological sciences and Biotechnology, Tsinghua University, Beijing, China.
Lei
L
Jin
Wenxiu
W
Zhao
Lan
L
Ma
D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D058953:Neural Stem Cells / Q000378:metabolism*; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*
D019151:Peptide Library; D010455:Peptides; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase