Detection and molecular characterization of bovine leukemia viruses from Jordan.
Bovine leukemia virus (BLV) is distributed worldwide. BLV has many effects on the health status and productivity of infected animals and is a potential risk for humans. In this study, we aimed to investigate the presence of and genotype bovine leukemia viruses on Jordanian dairy farms. Nested PCR coupled with RFLP and direct sequencing of a partial fragment of the env gene were carried out. Two BLV genotypes were found, genotypes 1 and 6. These genotypes were identified by nested PCR-RFLP of 444 bp of the env gene by restriction digestion with HaeIII, Bcl I and Pvu II. However, BLV-Jordan-10 seems to represent an entirely new genotype in our phylogenetic analysis. The nucleotide sequence identity between these two Jordanian BLV genotypes (1 and 6) was 96.2 %. The nucleotide sequence identity between Jordanian BLV genotype 1 and other reference BLV genotype 1 strains ranged from 99 % to 99.5 %. The nucleotide sequence similarity of the Jordanian BLV genotype 6 to other BLV genotypes ranged from 90 % to 96.7 %. A neutralizing motif and CD8(+) T-cell epitope were found in the env protein of both Jordanian isolates. In this study, we documented the presence of two BLV genotypes (1 and 6) on Jordanian dairy farms.
Department of Basic Veterinary Medical Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan. ababnem@just.edu.jo
Mustafa M
MM
Ababneh
Raida K
RK
Al-Rukibat
Wael M
WM
Hananeh
Abdelrahman T
AT
Nasar
Mohammad B
MB
Al-Zghoul
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000453:epidemiology / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D007597:Jordan / Q000453:epidemiology; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014777:Virus Diseases / Q000235:genetics
D014759:Viral Envelope Proteins