Bone formation using β-tricalcium phosphate/carboxymethyl-chitin composite scaffold in rat calvarial defects.
OBJECTIVE
The aim of the present study was to evaluate the effects of β-tricalcium phosphate/carboxymethyl-chitin material (β-TCP/CM-chitin) on bone formation in rat calvarial defects.


METHODS
Eighteen animals surgically received 2 calvarial defects (5 mm) bilaterally in each parietal bone. β-TCP/CM-chitin was implanted in one side of each defect, and the contralateral side of the defect was left empty as a control. The animals were humanely killed at 4, 8, and 12 weeks after surgery for histologic evaluation.


RESULTS
New bone formation in the β-TCP/CM-chitin group was significantly greater than that in the control group throughout the healing periods (P < .05). β-TCP/CM-chitin was remarkably resorbed 12 weeks after surgery.


CONCLUSIONS
These results indicate that β-TCP/CM-chitin is useful as a scaffold for bone formation.
Postgraduate Student, Department of Periodontology, Kagoshima University, Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Katsuyoshi
K
Taniyama
Yoshinori
Y
Shirakata
Takehiko
T
Yoshimoto
Naoshi
N
Takeuchi
Yusuke
Y
Yoshihara
Kazuyuki
K
Noguchi
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D018786:Bone Substitutes / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000494:pharmacology*; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004337:Drug Carriers / Q000494:pharmacology; D004343:Drug Implants / Q000494:pharmacology; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000187:drug effects* / Q000601:surgery; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000187:drug effects
D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; D004337:Drug Carriers; D004343:Drug Implants; C485817:beta-tricalcium phosphate; D002686:Chitin; C044872:O-(carboxymethyl)chitin