Short communication: evaluation of bovine milk residues from routine milk testing programs as DNA source for genotyping.
Genome-wide association studies and genomic evaluation using a dense set of genetic markers both require a large number of genotyped individuals. Collection of the respective samples contributes substantially to the cost of the approach. In dairy cattle research, the use of residues from routine milk recording would be a cost-saving alternative to obtain samples for an appropriate number of individuals with specific phenotypes in a very short time. To assess the suitability of milk recording residues, we concurrently investigated milk residues obtained after standardized milk recording procedures and blood samples from 115 cows originating from 3 farms with different milking systems by genotyping 15 microsatellite markers. We found that 4% of the milk samples were possibly assigned to the wrong animal (i.e., conflicts) and that at least 27% of the milk residues were contaminated, as indicated by an extra allele not present in the blood sample. These additional alleles primarily originated from a sample with a higher somatic cell score that went through the milk sample analyzer in the milk laboratory before the target sample. Furthermore, additional allele carryover was observed across more than one sample, when the difference in somatic cell count between samples exceeded 100,000 cells/mL. Finally, in several samples, the extra allele could not be traced back to previous samples passing through the milk sample analyzer. One source of those contaminations might be sample collection on-farm due to milk traces from the previously milked cow in the hose. No correlation was found between the farm management and conflicts or contaminations. We conclude that residues from routine milk recording are not suitable for genomic evaluation or genome-wide association studies because of the high prevalence of contamination generated at several steps during the collection and processing of milk residual samples.
Research Unit Molecular Biology, Leibniz Institute for Farm Animal Biology, 18196 Dummerstorf, Germany.
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Krappmann
Chair of Animal Breeding, Technische Universität München, 85354 Freising, Germany.
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Wurmser
Research Unit Genetics and Biometry, Leibniz Institute for Farm Animal Biology, 18196 Dummerstorf, Germany.
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Repsilber
Chair of Animal Breeding, Technische Universität München, 85354 Freising, Germany.
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Fries
Research Unit Molecular Biology, Leibniz Institute for Farm Animal Biology, 18196 Dummerstorf, Germany.
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Weikard
Landeskontrollverband für Leistungs- und Qualitätsprüfung Mecklenburg, 18273, Güstrow, Germany.
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Kesting
Research Unit Molecular Biology, Leibniz Institute for Farm Animal Biology, 18196 Dummerstorf, Germany. Electronic address: kuehn@fbn-dummerstorf.de.
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Kühn
D000818:Animals; D002417:Cattle / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D003612:Dairying / Q000379:methods; D005819:Genetic Markers / Q000235:genetics; D055106:Genome-Wide Association Study / Q000379:methods / Q000662:veterinary; D060005:Genotyping Techniques / Q000662:veterinary*; D018895:Microsatellite Repeats / Q000235:genetics; D008892:Milk / Q000737:chemistry*; D010641:Phenotype
D005819:Genetic Markers; D004247:DNA