Effects of penta-O-galloyl-β-D-glucose on human neutrophil function: significant down-regulation of L-selectin expression.
Penta-O-galloyl-β-D-glucose (PGG) occurrs in high concentrations in medicinal herbs such as Rhus chinensis, Paeonia suffruticosa, Acer truncatum and Terminalia chebula, which demonstrate anti-inflammatory activity. We investigated the effect of PGG on stimulated and non-stimulated neutrophils in processes which included reactive oxygen species generation (ROS), metalloproteinase-9 and interleukin-8 secretion (IL-8), β₂ integrin (CD11b) and L-selectin (CD62L) expression and apoptosis. In concentrations of 5 μM-20 μM, PGG demonstrated statistically significant inhibition of ROS generation, IL-8 secretion and β₂ integrin expression in stimulated neutrophils. The inhibition of L-selectin expression by PGG resulted in prevention in neutrophils' endothelial attachment. The result obtained may explain the anti-inflammatory activity of this compound and underline the contribution of PGG in the activity of PGG rich plant extracts.
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Faculty of Pharmacy, Medical University of Warsaw, Poland. akiss@wum.edu.pl
Anna K
AK
Kiss
Agnieszka
A
Filipek
Barbara
B
Zyżyńska-Granica
Marek
M
Naruszewicz
D031002:Acer / Q000737:chemistry; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D039481:CD11b Antigen / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D016209:Interleukin-8 / Q000096:biosynthesis*; D019041:L-Selectin / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D029596:Paeonia / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D029045:Rhus / Q000737:chemistry; D029766:Terminalia / Q000737:chemistry
D000893:Anti-Inflammatory Agents; D039481:CD11b Antigen; D047348:Hydrolyzable Tannins; C509295:ITGAM protein, human; D016209:Interleukin-8; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D019041:L-Selectin; C435084:pentagalloylglucose; D020780:Matrix Metalloproteinase 9; D005947:Glucose