Simultaneous UPLC analysis of three major flavonoids in granule decoctions of Fructus aurantii-type formulae.
A simple, rapid, and sensitive liquid chromatographic method has been established for the simultaneous analysis of three compounds (narirutin, hesperidin and naringin), in granule decoctions of Fructus Aurantii-type formulae. The compounds were separated in less than 10 min using a C18 column with gradient elution using (A) acetonitrile, (B) water, and (C) acetic acid at a flow rate of 0.3 mL/min, and with a PDA detector. The method was validated for specificity, accuracy, precision, and limits of detection. Good linear regression data (r2 > 0.9980) were obtained for all the calibration plots within the ranges tested. The method is an attractive alternative for quality control and clinical monitor of granule decoctions of Fructus Aurantii-type formulae.
Laboratory of Ethnopharmacology, Institute of Integrated Traditional Medicine and Western Medicine, Ganzhou, China.
Wei
W
Huang
Zhi-Hua
ZH
Xiong
Xi
X
Huang
Xiao
X
Chen
Wei-Ping
WP
Liu
Yang
Y
Wang
Ping
P
Ren
D002240:Carbohydrate Sequence; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002957:Citrus / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis*; D006569:Hesperidin / Q000032:analysis; D007202:Indicators and Reagents; D057230:Limit of Detection; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000737:chemistry; D012015:Reference Standards; D012044:Regression Analysis; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D004187:Disaccharides; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D007202:Indicators and Reagents; D010936:Plant Extracts; D012996:Solutions; C500601:narirutin; D006569:Hesperidin; C005274:naringin