Design and concise synthesis of a novel type of green fluorescent protein chromophore analogue.
A small molecular model compound for the green fluorescent protein chromophore was readily synthesized by a novel condensation reaction of (thio)imidate with imino-ester via an aziridine intermediate. This compound showed fluorescence in the solid and frozen solution states but not in the solution state. Its fluorescent property was successfully applied in the detection of dsDNA.
Faculty of Pharmacy, Osaka Ohtani University, Nishikiori-Kita 3-11-1, Tondabayashi, Osaka 584-8540, Japan.
Masahiro
M
Ikejiri
Moe
M
Tsuchino
Yoshiko
Y
Chihara
Takao
T
Yamaguchi
Takeshi
T
Imanishi
Satoshi
S
Obika
Kazuyuki
K
Miyashita
D001388:Aziridines / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D008958:Models, Molecular; D015394:Molecular Structure; D012996:Solutions
D001388:Aziridines; D005456:Fluorescent Dyes; D012996:Solutions; D049452:Green Fluorescent Proteins; D004247:DNA