Evaluation of a simple Theileria annulata culture protocol from experimentally infected bovine whole blood.
We have evaluated a new simple technique using whole blood from experimentally infected cattle for the isolation and cultivation of Theileria annulata. The study was carried out on 20 Holstein-Frisian bovines that had been experimentally infected with a virulent lethal dose of Theileria annulata. This technique has been compared to the classical peripheral blood monocyte isolation with Ficoll carried out on 22 experimentally infected Holstein-Friesian calves. The effectiveness of the reference technique was estimated to 86.4%, whilst the effectiveness of the new technique was 100%. Moreover, this new technique leads to time and money saving estimated to € 3.06 per sample. It decreases the contamination risks by reducing the steps of sample manipulation.
Laboratoire de Parasitologie, Université de la Manouba, Ecole Nationale de Médecine Vétérinaire, 2020 Sidi Thabet, Tunisie. gharbim2000@yahoo.fr
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Gharbi
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Latrach
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Sassi
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Darghouth
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000469:parasitology*; D046508:Culture Techniques / Q000191:economics / Q000379:methods / Q000592:standards / Q000662:veterinary*; D016207:Cytokines / Q000378:metabolism; D005362:Ficoll; D008214:Lymphocytes / Q000276:immunology; D018512:Parasitemia / Q000469:parasitology*; D010274:Parasitology / Q000191:economics / Q000379:methods / Q000592:standards; D016796:Theileria annulata / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D013801:Theileriasis / Q000097:blood / Q000469:parasitology*; D013997:Time Factors
D016207:Cytokines; D005362:Ficoll