Dioxin exposure reduces the steroidogenic capacity of mouse antral follicles mainly at the level of HSD17B1 without altering atresia.
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a potent ovarian toxicant. Previously, we demonstrated that in vitro TCDD (1nM) exposure decreases production/secretion of the sex steroid hormones progesterone (P4), androstenedione (A4), testosterone (T), and 17β-estradiol (E2) in mouse antral follicles. The purpose of this study was to determine the mechanism by which TCDD inhibits steroidogenesis. Specifically, we examined the effects of TCDD on the steroidogenic enzymes, atresia, and the aryl hydrocarbon receptor (AHR) protein. TCDD exposure for 48h increased levels of A4, without changing HSD3B1 protein, HSD17B1 protein, estrone (E1), T or E2 levels. Further, TCDD did not alter atresia ratings compared to vehicle at 48h. TCDD, however, did down regulate the AHR protein at 48h. TCDD exposure for 96h decreased transcript levels for Cyp11a1, Cyp17a1, Hsd17b1, and Cyp19a1, but increased Hsd3b1 transcript. TCDD exposure particularly lowered both Hsd17b1 transcript and HSD17B1 protein. However, TCDD exposure did not affect levels of E1 in the media nor atresia ratings at 96h. TCDD, however, decreased levels of the proapoptotic factor Bax. Collectively, these data suggest that TCDD exposure causes a major block in the steroidogenic enzyme conversion of A4 to T and E1 to E2 and that it regulates apoptotic pathways, favoring survival over death in antral follicles. Finally, the down-regulation of the AHR protein in TCDD exposed follicles persisted at 96h, indicating that the activation and proteasomal degradation of this receptor likely plays a central role in the impaired steroidogenic capacity and altered apoptotic pathway of exposed antral follicles.
Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, Urbana, IL 61802, USA. bklement@illinois.edu
Bethany N
BN
Karman
Mallikarjuna S
MS
Basavarajappa
Patrick
P
Hannon
Jodi A
JA
Flaws
D015067:17-Hydroxysteroid Dehydrogenases / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003577:Cytochrome P-450 Enzyme System; D015536:Down-Regulation / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D005496:Follicular Atresia / Q000187:drug effects*; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D051379:Mice; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D018336:Receptors, Aryl Hydrocarbon / Q000187:drug effects / Q000235:genetics; D013997:Time Factors; D051028:bcl-2-Associated X Protein / Q000378:metabolism
C494827:Bax protein, mouse; D004785:Environmental Pollutants; D012739:Gonadal Steroid Hormones; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D051028:bcl-2-Associated X Protein; D003577:Cytochrome P-450 Enzyme System; D015067:17-Hydroxysteroid Dehydrogenases; C576264:hydroxysteroid (17-beta) dehydrogenase 1, mouse