A human fallopian tube model for investigation of C. trachomatis infections.
Genital tract infections with Chlamydia trachomatis (C. trachomatis) are the most frequent transmitted sexually disease in women worldwide. Inefficient clearance or persistence of the pathogens may lead to ascending infections of the upper genital tract and are supposed to cause chronic inflammatory damage to infected tissues (1,2). As a consequence, severe clinical sequelae like pelvic inflammatory disease (PID), tubal occlusion and infertility may occur (3,4). Most of the research with C. trachomatis has been conducted in epithelial cell lines (e.g. HEp-2 cells and HeLa-229) or in mice. However, as with cell- culture based models, they do neither reflect the physiology of native tissue nor the pathophysiology of C. trachomatis genital tract infections in vivo (5). Further limitations are given by the fact that central signaling cascades (e.g. IFN-γ mediated JAK/STAT signaling pathway) that control intracellular chlamydial growth fundamentally differ between mice and humans (6,7). We and others therefore established a whole organ fallopian tube model to investigate direct interactions between C. trachomatis and human fallopian tube cells ex vivo (8,9). For this purpose, human fallopian tubes from women undergoing hysterectomy were collected and infected with C. trachomatis serovar D. Within 24 h post infection, specimen where analyzed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to detect Chlamydia trachomatis mediated epithelial damage as well as C. trachomatis inclusion formation in the fallopian tissue.
Institute of Medical Microbiology and Hygiene, University of Lübeck.
Stefan
S
Jerchel
Gudrun
G
Knebel
Peter
P
König
Michael K
MK
Bohlmann
Jan
J
Rupp
D002690:Chlamydia Infections / Q000382:microbiology / Q000473:pathology*; D002692:Chlamydia trachomatis / Q000472:pathogenicity*; D005184:Fallopian Tube Diseases / Q000382:microbiology* / Q000473:pathology*; D005187:Fallopian Tubes / Q000382:microbiology* / Q000473:pathology* / Q000601:surgery; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques