Overexpression of rice sphingosine-1-phoshpate lyase gene OsSPL1 in transgenic tobacco reduces salt and oxidative stress tolerance.
Sphingolipids, including sphingosine-1-phosphate (S1P), have been shown to function as signaling mediators to regulate diverse aspects of plant growth, development, and stress response. In this study, we performed functional analysis of a rice (Oryza sativa) S1P lyase gene OsSPL1 in transgenic tobacco plants and explored its possible involvement in abiotic stress response. Overexpression of OsSPL1 in transgenic tobacco resulted in enhanced sensitivity to exogenous abscisic acid (ABA), and decreased tolerance to salt and oxidative stress, when compared with the wild type. Furthermore, the expression levels of some selected stress-related genes in OsSPL1-overexpressing plants were reduced after application of salt or oxidative stress, indicating that the altered responsiveness of stress-related genes may be responsible for the reduced tolerance in OsSPL1-overexpressing tobacco plants under salt and oxidative stress. Our results suggest that rice OsSPL1 plays an important role in abiotic stress responses.
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310058, China.
Huijuan
H
Zhang
Jing
J
Zhai
Jibo
J
Mo
Dayong
D
Li
Fengming
F
Song
D000040:Abscisic Acid / Q000494:pharmacology; D000446:Aldehyde-Lyases / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics*; D010269:Paraquat / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics*; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*
D010940:Plant Proteins; D012965:Sodium Chloride; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D000446:Aldehyde-Lyases; C022649:sphingosine 1-phosphate lyase (aldolase); D010269:Paraquat