Mouse ooplasm confers context-specific reprogramming capacity.
Enucleated oocytes have the distinctive ability to reprogram somatic nuclei back to totipotency. Here, we investigate genome-scale DNA methylation patterns after nuclear transfer and compare them to the dynamics at fertilization. We identify specific targets for DNA demethylation after nuclear transfer, such as germline-associated promoters, as well as unique limitations that include certain repetitive element classes.
Broad Institute of MIT, Cambridge, Massachusetts, USA.
Michelle M
MM
Chan
Zachary D
ZD
Smith
Dieter
D
Egli
Aviv
A
Regev
Alexander
A
Meissner
D000818:Animals; D003593:Cytoplasm / Q000502:physiology*; D019175:DNA Methylation / Q000502:physiology; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D009928:Organ Specificity; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology; D013084:Sperm-Ovum Interactions / Q000502:physiology