Rapamycin preconditioning attenuates transient focal cerebral ischemia/reperfusion injury in mice.
Rapamycin, an mTOR inhibitor and immunosuppressive agent in clinic, has protective effects on traumatic brain injury and neurodegenerative diseases. But, its effects on transient focal ischemia/reperfusion disease are not very clear. In this study, we examined the effects of rapamycin preconditioning on mice treated with middle cerebral artery occlusion/reperfusion operation (MCAO/R). We found that the rapamycin preconditioning by intrahippocampal injection 20 hr before MCAO/R significantly improved the survival rate and longevity of mice. It also decreased the neurological deficit score, infracted areas and brain edema. In addition, rapamycin preconditioning decreased the production of NF-κB, TNF-α, and Bax, but not Bcl-2, an antiapoptotic protein in the ischemic area. From these results, we may conclude that rapamycin preconditioning attenuate transient focal cerebral ischemia/reperfusion injury and inhibits apoptosis induced by MCAO/R in mice.
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou, PR China.
Lele
L
Yin
Shasha
S
Ye
Zhen
Z
Chen
Yaoying
Y
Zeng
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001929:Brain Edema / Q000209:etiology / Q000517:prevention & control; D020520:Brain Infarction / Q000209:etiology / Q000517:prevention & control; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology; D020244:Infarction, Middle Cerebral Artery / Q000097:blood / Q000401:mortality / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000097:blood; D009422:Nervous System Diseases / Q000209:etiology / Q000517:prevention & control; D009460:Neurologic Examination; D015427:Reperfusion Injury / Q000150:complications / Q000401:mortality / Q000517:prevention & control*; D020123:Sirolimus / Q000627:therapeutic use*; D015996:Survival Rate; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D051028:bcl-2-Associated X Protein / Q000097:blood
D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein; D020123:Sirolimus