Chemical composition and in vitro evaluation of antimicrobial and anti-acetylcholinesterase properties of the flower oil of Ferula lutea.
The present work describes the chemical composition and evaluates the antimicrobial and the anti-acetylcholinesterase properties of the flower oil from the Tunisian Ferula lutea obtained by hydrodistillation and analyzed by combination of GC/FID and GC/MS. The chemical composition of the flower oil of this species is reported for the first time. Seventeen compounds were identified accounting for 94.3% of the total oil. The chemical composition of this essential oil was characterized by a high proportion of monoterpene hydrocarbons (80.4%) among which delta-3-carene (31.2%) and alpha-pinene (25.8%) were the predominant compounds. The oxygenated monoterpenes represent the second major fraction (12.0%), 2,3,6-trimethylbenzaldehyde (10.9%) being the predominant one. Furthermore, the isolated oil was tested for its antimicrobial activity using the disc-diffusion and the microdilution assays against six Gram-positive and five Gram-negative bacteria as well as towards eight Candida species. It was found that flower oil of F. lutea exhibited interesting antibacterial and anticandidal activity (MIC = 39 mcirog/mL against Escherichia coli, Staphylococcus aureus and S. epidermidis and MIC = 156 microg/mL against Candida albicans). The anti-acetylcholinesterase effect of this oil was also evaluated in this work. Results showed that this oil exhibits significant activity (IC50 =70.25 +/- 5.41 microg/mL).
Département de Chimie, Faculté des Sciences de Monastir, Universite de Monastir, Avenue de l'Environnement, 5019 Monastir Tunisie.
Mansour
M
Znati
Aymen
A
Jabrane
Hafedh
H
Hajlaoui
Fethia
F
Harzallah-Skhiri
Jalloul
J
Bouajila
Joseph
J
Casanova
Hichem
H
Ben Jannet
D000110:Acetylcholinesterase / Q000378:metabolism; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D000081008:Bicyclic Monoterpenes; D005309:Ferula / Q000737:chemistry*; D035264:Flowers / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
D000890:Anti-Infective Agents; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D010938:Plant Oils; D000110:Acetylcholinesterase; C005451:alpha-pinene