In vitro studies to assess the antidiabetic, antiperoxidative, and radical scavenging potential of Stereospermum colais.
BACKGROUND
Stereospermum colais (Buch.-Ham. ex Dillw.) Mabberley (Bignoniaceae), which has traditional medicinal properties, is distributed all over deciduous forests. In spite of its many uses, the antidiabetic, antiperoxidative and radical scavenging activities of this species have not been assessed, and its chemical composition is scarcely known.


OBJECTIVE
Antidiabetic, antiperoxidation, xanthine oxidase (XO) inhibition, and radical scavenging activities of acetone and methanol extracts of Stereospermum colais roots were investigated. Protective effects of Stereospermum colais root extract in stabilizing sunflower oil was also examined.


METHODS
The protective effect of acetone (ASC) and methanol (MSC) extracts of Stereospermum colais root for the potential inhibition of α-glucosidase and α-amylase enzymes were studied by in vitro method. Glycation inhibitory activity was also studied to inhibit the production of glycated end products.


RESULTS
Compared with acarbose, ASC showed a strong inhibitory activity against α-glucosidase (IC(50) 61.21 µg/mL) and a moderate inhibitory activity against α-amylase (IC(50) 681.08 µg/mL). Glycation inhibitory activity of Stereospermum colais root extracts by using an in vitro glucose-bovine serum albumin (BSA) assay was also done and compared with standard gallic acid. ASC also shows high XO inhibition potential, free radical scavenging activities, and low p-anisidine value indicates the high medicinal potency of Stereospermum colais root.


CONCLUSIONS
These results suggest that the extract of Stereospermum colais may be interesting for incorporation in pharmaceutical preparations for human health, since it can suppress hyperglycaemia, and or as food additives due to its antiradical efficiency.
Agro processing & Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum, India
M Priya
MP
Rani
K P
KP
Padmakumari
D020909:Acarbose / Q000494:pharmacology; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D029402:Bignoniaceae / Q000737:chemistry*; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology*; D005947:Glucose / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry; D018517:Plant Roots; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D000074242:Sunflower Oil; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
D000975:Antioxidants; D016166:Free Radical Scavengers; D017127:Glycation End Products, Advanced; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D010938:Plant Oils; D012997:Solvents; D000074242:Sunflower Oil; D012710:Serum Albumin, Bovine; D000516:alpha-Amylases; D005947:Glucose; D020909:Acarbose