Monoclonal antibody selectively recognizing murine but not human CD44.
Here we report on the generation of a monoclonal antibody (MAb) specific for murine, but not human CD44 obtained by immunization with cancer-associated fibroblasts. The monoclonal antibody MS44 recognizes CD44, as evidenced by immunoblotting of cell lysates. Additionally, MAb MS44 reacts with mouse but not human CD44 in flow cytometry. Thus, this antibody provides an effective tool to analyze CD44 from host (mouse) cells in human cancer cell xenograft models in mice.
Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan.
Yumi
Y
Kinugasa
Nobuyuki
N
Takakura
D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000276:immunology*; D000918:Antibody Specificity; D019556:COS Cells; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000096:biosynthesis; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D051381:Rats; D011916:Rats, Inbred F344; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000096:biosynthesis
D058846:Antibodies, Monoclonal, Murine-Derived; D018960:Hyaluronan Receptors; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D049452:Green Fluorescent Proteins; D020797:Receptor, Platelet-Derived Growth Factor beta