Antitumour activity of Annona squamosa seed extracts is through the generation of free radicals and induction of apoptosis.
The plant, Annona squamosa traditionally known as custard apple possesses potent bioactive principles in all its parts. The effect of aqueous and organic extracts from defatted seeds of A. squamosa was studied on a rat histiocytic tumour cell line, AK-5. Both the extracts caused significant apoptotic tumour cell death with enhanced caspase-3 activity, down regulation of antiapoptotic genes Bcl-2 and Bcl(XL), and enhanced the generation of intracellular ROS, which correlated well with the decreased levels of intracellular GSH. In addition, DNA fragmentation and annexin-V staining confirmed that the extracts induced apoptosis in tumour cells through the oxidative stress. Aqueous extracts of A. squamosa seeds possessed significant antitumor activity in vivo against AK-5 tumor.
Centre for Cellular & Molecular Biology, Uppal Road, Hyderabad 500 007, India.
B V V
BV
Pardhasaradhi
Madhurima
M
Reddy
A Mubarak
AM
Ali
A Leela
AL
Kumari
Ashok
A
Khar
D000818:Animals; D031041:Annona / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D053938:DNA Fragmentation; D005434:Flow Cytometry / Q000379:methods; D005609:Free Radicals; D015971:Gene Expression Regulation, Enzymologic; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D009368:Neoplasm Transplantation; D018384:Oxidative Stress; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species; D012639:Seeds / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism
D000970:Antineoplastic Agents; D005609:Free Radicals; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D051020:bcl-X Protein; D053148:Caspase 3; D005978:Glutathione