Entrapment of Sorghum root oxalate oxidase into polyvinyl alcohol membrane.
A Cl- and NO3- insensitive oxalate oxidase, purified from the roots of 10-day old seedlings of grain Sorghum has been immobilized on polyvinyl alcohol (PVA) membrane through entrapment with 96.07% retention of initial activity. The membrane bound enzyme showed an increase in optimum pH (from 5.0 to 6.5), time of incubation (from 5 to 10 min) and Km for oxalate (from 0.38 to 6.23 mM), but decrease in incubation temperature for maximum activity (from 37 to 30 degrees C) and Vmax (from 70 nmol/min/ml to 9.7 nmol H2O2/min) and was unaffected by Cl- and NO3. The membrane bound enzyme lost 50% of its initial activity after 30 days of storage at room temperature. The use of membrane bound oxalate oxidase in determination of serum oxalate of urinary stone patients is demonstrated.
Biochemistry Research Laboratory, Dept. of BioSciences, M D University, Rohtak 124 001 (Haryana), India.
Geeta
G
Singh
Suman
Durgesh N
DN
Tanwar
C S
CS
Pundir
D002462:Cell Membrane / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010070:Oxalates / Q000737:chemistry*; D019815:Oxalic Acid / Q000737:chemistry; D010088:Oxidoreductases / Q000737:chemistry; D018517:Plant Roots / Q000378:metabolism*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D045868:Sorghum / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014545:Urinary Calculi / Q000737:chemistry
D010070:Oxalates; D011142:Polyvinyl Alcohol; D019815:Oxalic Acid; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; C020598:oxalate oxidase