MiR-195 regulates cell apoptosis of human hepatocellular carcinoma cells by targeting LATS2.
Recently, we have reported tissue- and stage-specific expression of miR-195 in human hepatocellular carcinoma cells and so far, not many reports discuss the function of this microRNA (miRNA). Expression profiling of miRNAs revealed a limited set of miRNAs with altered expression in drug resistant hepatocellular carcinoma cell line BEL-7402/5-FU compared to its parental BEL-7402 cell line. Real-time PCR confirmed down-regulation of miR-195 in BEL-7402/5-FU cells. Western blots were performed to determine protein levels of LATS2, P53 and CDK2. MTT analysed the cell proliferation activity. Flow cytometry were performed to determine apoptosis rate. Up-regulation of miR-195 increased expression of LATS2 and increased apoptosis of HCC cells, while Anti-miR-195 treatment inhibited expression of LATS2. miR-195 over-expression inhibited the luciferase activity of a LATS2 3' untranslated region-based reporter construct in BEL-7402/5-FU cells. These results indicate that miR-195 could increase cell apoptosis by targeting LATS2 in hepatocellular carcinoma cells.
Institute of Pharmacy and Pharmacology, The First Affilitated Hospital, University of South China Hengyang, P.R. China.
Xiaoyan
X
Yang
Jia
J
Yu
Jie
J
Yin
Qiong
Q
Xiang
Huifang
H
Tang
Xiaoyong
X
Lei
D020413:3' Untranslated Regions / Q000187:drug effects / Q000235:genetics; D000963:Antimetabolites / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation / Q000502:physiology; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D035683:MicroRNAs / Q000494:pharmacology*; D046228:Microarray Analysis; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000187:drug effects* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D025521:Tumor Suppressor Proteins / Q000187:drug effects* / Q000235:genetics
D020413:3' Untranslated Regions; D000963:Antimetabolites; C552722:MIRN195 microRNA, human; D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53; D025521:Tumor Suppressor Proteins; C406449:LATS2 protein, human; D017346:Protein Serine-Threonine Kinases; D005472:Fluorouracil