Techniques of digital image analysis for histological quantification of melanin.
Morphometric analysis of tissue melanin may quantitatively contribute to research on pigmentation disorders. The authors present three methods for image analysis, which allow for identification of melanin-equivalent pixels in the epidermis using Fontana-Masson stain and, therefore, for the calculation of its percentage in the different epidermal layers. Moreover, they discuss the main elements related to the analysis and the need for rigorous standardization of the process.
Department of Dermatology and Radiotherapy, School of Medicine of Botucatu, Universidade Estadual Paulista Julio de Mesquita Filho, Sao Paulo, Brazil. heliomiot@fmb.unesp.br
Hélio Amante
HA
Miot
Gabrielli
G
Brianezi
Andréia de Almeida
Ade A
Tamega
Luciane Donida Bartoli
LD
Miot
D004817:Epidermis / Q000737:chemistry* / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008543:Melanins / Q000032:analysis*; D010859:Pigmentation Disorders / Q000473:pathology*
D008543:Melanins