Response to arsenate treatment in Schizosaccharomyces pombe and the role of its arsenate reductase activity.
Arsenic toxicity has been studied for a long time due to its effects in humans. Although epidemiological studies have demonstrated multiple effects in human physiology, there are many open questions about the cellular targets and the mechanisms of response to arsenic. Using the fission yeast Schizosaccharomyces pombe as model system, we have been able to demonstrate a strong activation of the MAPK Spc1/Sty1 in response to arsenate. This activation is dependent on Wis1 activation and Pyp2 phosphatase inactivation. Using arsenic speciation analysis we have also demonstrated the previously unknown capacity of S. pombe cells to reduce As (V) to As (III). Genetic analysis of several fission yeast mutants point towards the cell cycle phosphatase Cdc25 as a possible candidate to carry out this arsenate reductase activity. We propose that arsenate reduction and intracellular accumulation of arsenite are the key mechanisms of arsenate tolerance in fission yeast.
Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid-Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Alejandro
A
Salgado
Ana
A
López-Serrano Oliver
Ana M
AM
Matia-González
Jael
J
Sotelo
Sonia
S
Zarco-Fernández
Riansares
R
Muñoz-Olivas
Carmen
C
Cámara
Miguel A
MA
Rodríguez-Gabriel
D053502:Arsenate Reductases / Q000378:metabolism*; D001149:Arsenates / Q000633:toxicity*; D018797:Cell Cycle Proteins / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D005838:Genotype; D015151:Immunoblotting; D013058:Mass Spectrometry; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000201:enzymology; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*; D013054:Spectrophotometry, Atomic
D001149:Arsenates; D018797:Cell Cycle Proteins; D029702:Schizosaccharomyces pombe Proteins; D053502:Arsenate Reductases; D020928:Mitogen-Activated Protein Kinases; C524689:Spc1 protein, S pombe; C095409:sty1 protein, S pombe; D020929:Mitogen-Activated Protein Kinase Kinases; C072068:wis1 protein, S pombe; C570820:Cdc25 protein, S pombe; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; C078759:pyp2 protein, S pombe; C025657:arsenic acid