Trypanosoma cruzi III from armadillos (Dasypus novemcinctus novemcinctus) from Northeastern Venezuela and its biological behavior in murine model. Risk of emergency of Chagas' disease.
Trypanosoma cruzi, etiological agent of Chagas' disease, was isolated from armadillos (Dasypus novemcinctus novemcinctus) captured in rural communities Northeastern Venezuela from Nueva Esparta State (no endemic for Chagas' disease), Monagas and Anzoátegui States (endemics). The isolates, genetically typed by PCR-RFLP as belonging to the TcIII DTU, have demonstrated in murine model heterogenic parasitemia, mortality and histotropism with marked parasitism in cardiac, skeletal, and smooth myocytes that showed correlation with lymphobasophilic inflammatory infiltrates. Our finding of T. cruzi infected armadillos in Isla Margarita (Nueva Esparta State), together with reports of triatomine vectors in this region, the accentuated synanthropy of armadillos, intense economic activity, migration due to tourism and the lack of environmental education programs all of them represent risks that could cause the emergence of Chagas' disease in this area. This is the first report of the TcIII DTU in Northeastern Venezuela, thus widening the geographic distribution of this DTU.
Eastern University, Eastern Center for Tropical Medicine, School of Health Sciences, Anzoátegui State, Venezuela. amorocoima@gmail.com
Antonio
A
Morocoima
Hernán J
HJ
Carrasco
Johanna
J
Boadas
José David
JD
Chique
Leidi
L
Herrera
Servio
S
Urdaneta-Morales
D000818:Animals; D001135:Armadillos / Q000469:parasitology*; D014355:Chagas Disease / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D004195:Disease Models, Animal; D004197:Disease Reservoirs; D005260:Female; D008297:Male; D051379:Mice; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D014687:Venezuela / Q000453:epidemiology