Downregulation of CCR5 expression on the peripheral blood CD8+ T cells of southeastern Iranian patients with chronic hepatitis B infection.
Studies indicated that CC receptor 5 (CCR5), as a receptor for CC ligand 3, CCL4, and CCL5, plays important roles in the recruitment of T cytotoxic lymphocytes to the liver of chronic HBV (CHB)-infected patients. The main purpose of this study was to investigate the expression levels of CCR5 on the CD8(+) T lymphocytes of CHB patients. This clinical study was performed on 63 CHB patients and 96 healthy controls. Flow cytometric analysis was performed to examine the expression of CCR5 on CD8(+) T cells of CHB patients. Real-time PCR was also used for HBV-DNA quantification. The results of our study demonstrated that CCR5 expressing T cytotoxic cells were decreased significantly in CHB patients in comparison to healthy control. Based on our results, it can be concluded that the percent of CCR5(+)/CD8(+) T cells in Iranian CHB patients is significantly decreased, hence their migration to the infected liver, and HBV eradication from the hepatocytes is disrupted.
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Behzad Nasiri
BN
Ahmadabadi
Gholamhossein
G
Hassanshahi
Hossein
H
Khoramdelazad
Vahid
V
Mirzaei
Seyed Mohammad Ali
SM
Sajadi
Masomeh
M
Hajghani
Hassan
H
Khodadadi
Reza
R
Pourali
Mohammad Kazemi
MK
Arababadi
Derek
D
Kennedy
D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002465:Cell Movement; D004279:DNA, Viral / Q000032:analysis; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D006515:Hepatitis B virus / Q000276:immunology* / Q000302:isolation & purification; D019694:Hepatitis B, Chronic / Q000097:blood / Q000276:immunology* / Q000821:virology; D006801:Humans; D007492:Iran; D008099:Liver / Q000276:immunology / Q000821:virology; D008111:Liver Function Tests; D008297:Male; D019713:Receptors, CCR5 / Q000096:biosynthesis*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism
D004279:DNA, Viral; D019713:Receptors, CCR5; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D001663:Bilirubin