Comparison of commercial serum-free media for CHO-K1 cell growth and monoclonal antibody production.
The selection of a serum-free medium for a particular process of production using mammalian cells is a critical step for its success. In this study, seven commercially available serum-free media (EX-CELL, ISF-I, CD CHO, CDM4CHO, CHO-III-A, Octomed and HybridoMed) were evaluated and compared for cell growth and monoclonal antibody (mAb) production of a transfected CHO-K1 cell line. In the conditions assayed, EX-CELL and particularly CDM4CHO are the most recommended media for extended biopharmaceutical processes, on account of inducing superior levels of cell proliferation and mAb production, accentuated by a tendency to improve over time. Furthermore, the less positive results obtained with some media emphasize the importance and the impact of the correct medium selection.
IBB - Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Maria Elisa
ME
Rodrigues
Ana Rita
AR
Costa
Mariana
M
Henriques
Joana
J
Azeredo
Rosário
R
Oliveira
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D016466:CHO Cells; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D016895:Culture Media, Serum-Free
D000911:Antibodies, Monoclonal; D016895:Culture Media, Serum-Free