Amperometric biosensor based on 3D ordered freestanding porous Pt nanowire array electrode.
A three-dimensionally (3D) ordered freestanding porous platinum (Pt) nanowire array electrode (PPNWAE) with pores of several nanometers in size and a Pt nanowire array electrode (PNWAE) without pores were facilely fabricated by metal electrodeposition and direct integration with a Pt disk electrode. The unusual PPNWAE with high active area showed excellent sensitivity (0.36 mA cm(-2) mM(-1)) and a wide detection range (4.5 μM-27.1 mM) to hydrogen peroxide (H(2)O(2)). A glucose oxidase (GOD)-based biosensor (PPNWAE/GOD) with a considerably wide detection range (4.5 μM-189.5 mM) to glucose was demonstrated. Furthermore, a lower detection limit, higher sensitivity and smaller value of Michaelis-Menten constant k(m) were recorded for PPNWAE-based biosensors compared with PNWAE-based biosensors. Particularly, the response current to glucose of PPNWAE/GOD was ca. 100% higher than that of PNWAE/GOD and the response current to H(2)O(2) of PPNWAE was ca. 50% higher than that of PNWAE, owing to the granular and rougher porous nanowire surface enabling greater bioactivity for GOD. The selectivity of PPNWAE/GOD glucose biosensor was also estimated.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
Yunli
Y
Wang
Yingchun
Y
Zhu
Jingjing
J
Chen
Yi
Y
Zeng
D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D016062:Porosity
D004800:Enzymes, Immobilized; D010984:Platinum; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose