[Molecular and epidemiological study on among children under 5 years old in Nanjing].
OBJECTIVE
To study the infected information, clinical symptom and molecular epidemiological characteristics of HuCV infection among children under 5 years old in Nanjing.


METHODS
In Nanjing Children's Hospital of Nanjing Medical University from July 2010 to June 2011, we collected 428 stool specimens from children with diarrhea and 428 asymptomatic controls. Human Calicivirus were tested by using RT-PCR. Then we sequenced the nucleic acid of PCR amplifications and identified the genotype and gene group of prevalent strains.


RESULTS
63 (14.72%) out of 428 stool samples were detected as HuCV. 58 were norovirus and 5 were sapovirus, while GII-4 2006b was the predominant strain of NoV. In the 428 control samples, 19 samples were positive for calicivirus, there were 8 NoV and 13 SaV (Including 3 co-infection cases).


CONCLUSIONS
Human caliciviruses with different genotypes circulated among children in Nanjing,and GII. 2006b is the dominant genotype.
Medical School of Nanjing University, Nanjing 210093, China.
Xiao-Le
XL
Li
Dan-Di
DD
Li
Wei-Xia
WX
Cheng
Guang-Cheng
GC
Xie
Xiao-Qian
XQ
Gao
Gui-Ping
GP
Kong
Yu
Y
Jin
Zhao-Jun
ZJ
Duan
D002139:Caliciviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017250:Caliciviridae Infections / Q000453:epidemiology* / Q000821:virology; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D017720:Molecular Epidemiology; D010802:Phylogeny; D012621:Seasons