Efficacy of the stannous ion and a biopolymer in toothpastes on enamel erosion/abrasion.
OBJECTIVE
Sn(2+) has promising erosion-inhibiting properties in solutions, but little is known about respective effects in toothpastes. In addition, biopolymers might have protecting potential. Aim of this study was to investigate the effects of Sn(2+) in toothpastes and of a biopolymer (chitosan) added to a Sn(2+) formulation on erosion/abrasion.


METHODS
Enamel samples were subjected to cyclic erosion procedures (10 days; 0.50% citric acid, pH 2.5; 6× 2 min/day), and brushing (2× 15s/day, load 200 g) during immersion in slurries (2 min). The toothpastes were NaF formulations (NaF/1, NaF/2, NaF/3) and Sn(2+) formulations (NaF/SnCl(2), AmF/SnF(2), AmF/NaF/SnCl(2)) and AmF/NaF/SnCl(2)+0.5% chitosan. Declared concentrations of active ingredients in toothpastes were 1400-1450 μg/g F(-) and 3280-3500 μg/g Sn(2+). Negative controls were erosion only and placebo, positive control was a SnF(2) gel. Tissue loss was quantified profilometrically, Sn on enamel surfaces was measured by energy dispersive X-ray spectroscopy.


RESULTS
Loss values (μm) for erosion only and placebo were 14.4 ± 4.5 and 20.2 ± 3.8, respectively, and 4.6 ± 1.9 for the positive control (p ≤ 0.001 each compared to erosion only). The other loss values were: NaF/1 16.5 ± 3.0, NaF/2 14.0 ± 2.7, NaF/3 12.6 ± 3.9, NaF/SnCl(2) 14.7 ± 5.1, AmF/SnF(2) 13.5 ± 4.8, AmF/NaF/SnCl(2) 12.4 ± 4.2, AmF/NaF/SnCl(2)+chitosan 6.6 ± 3.5 (except NaF/1 all p ≤ 0.01 compared to placebo). AmF/NaF/SnCl(2)/chitosan was more effective than all other toothpastes (p ≤ 0.01 each). Sn on the enamel surface ranged between 1.3 ± 0.3 and 2.8 ± 0.04 wt.% with no obvious relationship with efficacy.


CONCLUSIONS
The NaF and Sn(2+) toothpastes without chitosan exhibited similar anti-erosion and abrasion-prevention effects. The experimental Sn(2+) formulation with chitosan revealed promising results similar to those of the positive control.


CONCLUSIONS
NaF toothpastes offer a degree of protection against erosion/abrasion, which is likely sufficient for most subjects with average acid exposures. For patients with initial erosive lesions, however, more effective toothpaste is desirable. The combination of Sn(2+) and a biopolymer appears promising in this context.
Department of Conservative and Preventive Dentistry, Dental Clinic of the Justus-Liebig-University Giessen, Germany. carolina.ganss@dentist.med.uni-giessen.de
C
C
Ganss
J
J
von Hinckeldey
A
A
Tolle
K
K
Schulze
J
J
Klimek
N
N
Schlueter
D000588:Amines / Q000494:pharmacology; D048271:Chitosan / Q000494:pharmacology*; D019343:Citric Acid / Q000009:adverse effects; D003743:Dental Enamel / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017736:Ion-Selective Electrodes; D008422:Materials Testing; D010919:Placebos; D012969:Sodium Fluoride / Q000494:pharmacology; D013052:Spectrometry, X-Ray Emission; D014001:Tin / Q000032:analysis; D017971:Tin Compounds / Q000494:pharmacology*; D014002:Tin Fluorides / Q000494:pharmacology; D014072:Tooth Abrasion / Q000473:pathology* / Q000517:prevention & control; D014077:Tooth Erosion / Q000473:pathology* / Q000517:prevention & control; D014099:Toothbrushing / Q000009:adverse effects; D014100:Toothpastes / Q000494:pharmacology*
D000588:Amines; D010919:Placebos; D017971:Tin Compounds; D014002:Tin Fluorides; D014100:Toothpastes; C023599:stannous chloride; D019343:Citric Acid; C075174:amine fluoride gel; D014001:Tin; D012969:Sodium Fluoride; D048271:Chitosan