Circulating CD35(-)/CD49d(+) neutrophils in influenza virus infection patients.
Release of immature neutrophils from bone marrow into circulation accompanies acute inflammatory states. Previous studies have identified a number of antigens that are differentially expressed on mature neutrophils and immature forms. We analyzed expression of these antigens on circulating neutrophils of influenza virus infection patients. We identified a minor neutrophil subpopulation with decreased granularity and the antigen surface expression pattern (CD35(-)/CD49d(+)) which defines metamyelocytes. CD35(-)/CD49d(+) neutrophils were detected in 35% of the patients but not in healthy individuals and correlated positively with blood metamyelocyte count. The sorted CD35(-)/CD49d(+) neutrophils displayed morphological features of metamyelocytes. Compared with a major (CD35(+)/CD49d(-)) neutrophil subpopulation, CD35(-)/CD49d(+) neutrophils demonstrated significantly diminished functional capacities (both phagocytosis and respiratory burst). Thus, CD35(-)/CD49d(+) neutrophils represent a phenotypically and functionally distinct subpopulation of the cells and can potentially be used to quantify immature neutrophil release in inflammation.
Laboratory of Clinical Immunology, Institute of Immunology, Kashirskoye Shosse 24-2, Moscow 115478, Russia. bpliyev@cardio.ru
Boris K
BK
Pliyev
Evgeniya I
EI
Shmidt
Anna V
AV
Ivanova
Mikhail
M
Menshikov
D000328:Adult; D016022:Case-Control Studies; D006801:Humans; D016130:Immunophenotyping; D007251:Influenza, Human / Q000276:immunology*; D039441:Integrin alpha4 / Q000378:metabolism; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009975:Orthomyxoviridae / Q000276:immunology*; D017463:Receptors, Complement 3b / Q000378:metabolism; D055815:Young Adult
D017463:Receptors, Complement 3b; D039441:Integrin alpha4