Measurement of glucose homeostasis in vivo: combination of tracers and clamp techniques.
A tracer technique referred to as "pancreatic-blood glucose clamp" allows assessment in response to a change in blood glucose, insulin, and/or glucagon of whole body glucose disposal, endogenous glucose production, specific tissue/organ glucose uptake and storage, and insulin secretion. This technique is currently considered the optimal method for measurement of insulin sensitivity and glucose effectiveness. We describe here, for use in conscious-unrestrained mice and rats, the pancreatic-blood glucose clamp technique and its associated methods; which include catheterization of blood vessels; a clamp of plasma insulin, glucagon, and glucose; analyses of metabolites and tracers; and calculations.
Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN, USA. Masakazu.shiota@vanderbilt.edu
Masakazu
M
Shiota
D000818:Animals; D001786:Blood Glucose / Q000032:analysis* / Q000378:metabolism; D002404:Catheterization / Q000295:instrumentation / Q000379:methods; D002408:Catheters, Indwelling; D004867:Equipment Design; D005934:Glucagon / Q000097:blood; D005947:Glucose / Q000378:metabolism*; D015309:Glucose Clamp Technique / Q000379:methods*; D006003:Glycogen / Q000378:metabolism; D006706:Homeostasis; D007328:Insulin / Q000097:blood* / Q000378:metabolism; D007333:Insulin Resistance; D051379:Mice; D010179:Pancreas / Q000378:metabolism; D051381:Rats; D014656:Vascular Surgical Procedures / Q000295:instrumentation / Q000379:methods
D001786:Blood Glucose; D007328:Insulin; D006003:Glycogen; D005934:Glucagon; D005947:Glucose