A general latent class model for performance evaluation of diagnostic tests in the absence of a gold standard: an application to Chagas disease.
We propose a new general Bayesian latent class model for evaluation of the performance of multiple diagnostic tests in situations in which no gold standard test exists based on a computationally intensive approach. The modeling represents an interesting and suitable alternative to models with complex structures that involve the general case of several conditionally independent diagnostic tests, covariates, and strata with different disease prevalences. The technique of stratifying the population according to different disease prevalence rates does not add further marked complexity to the modeling, but it makes the model more flexible and interpretable. To illustrate the general model proposed, we evaluate the performance of six diagnostic screening tests for Chagas disease considering some epidemiological variables. Serology at the time of donation (negative, positive, inconclusive) was considered as a factor of stratification in the model. The general model with stratification of the population performed better in comparison with its concurrents without stratification. The group formed by the testing laboratory Biomanguinhos FIOCRUZ-kit (c-ELISA and rec-ELISA) is the best option in the confirmation process by presenting false-negative rate of 0.0002% from the serial scheme. We are 100% sure that the donor is healthy when these two tests have negative results and he is chagasic when they have positive results.
Department of Education and Nursing Community Health, Federal University of Triângulo Mineiro, 38025-180 Uberaba, MG, Brazil.
Gilberto
G
de Araujo Pereira
Francisco
F
Louzada
Valdirene
V
de Fátima Barbosa
Márcia Maria
MM
Ferreira-Silva
Helio
H
Moraes-Souza
D000465:Algorithms; D000818:Animals; D001499:Bayes Theorem; D014355:Chagas Disease / Q000175:diagnosis* / Q000469:parasitology; D019295:Computational Biology / Q000379:methods; D003955:Diagnostic Tests, Routine / Q000379:methods / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005188:False Negative Reactions; D005189:False Positive Reactions; D006801:Humans; D015233:Models, Statistical; D011336:Probability; D012680:Sensitivity and Specificity; D012984:Software