Calorimetry-based profiling of blood plasma from colorectal cancer patients.
BACKGROUND
Differential scanning calorimetry (DSC), a highly sensitive technique for resolving thermally-induced protein folding/unfolding transitions, recently was recognized as a novel tool for disease diagnosis and monitoring. To further elaborate this approach we have applied DSC in a study of blood plasma from patients with colorectal cancer (CRC) at different stages of tumor development and localization.


METHODS
Blood plasma from patients diagnosed with CRC was analyzed by DSC. The CRC thermograms were compared to those of healthy individuals and patients with gastric cancer and non-cancerous soft tissue inflammation. The thermodynamic parameters: excess heat capacity and enthalpy of the transitions corresponding to the most abundant plasma proteins, as well as transition and first moment temperatures were determined from the calorimetric profiles.


RESULTS
The calorimetric profiles of blood plasma from CRC patients are found to be distinct from those of healthy individuals and those of patients with soft tissue, non-cancerous inflammation. Generally the CRC thermograms exhibit reduced heat capacity of the major albumin/globulin-assigned thermal transitions, lower enthalpy and a featureless high temperature region compared to healthy individuals.


CONCLUSIONS
A classification of blood plasma proteome from patients with colorectal cancer (CRC1, CRC2 and CRC3 groups, and subgroups within each group CRC1(1-2), CRC2(1-2) and CRC3(1-2)) is proposed based on the derived thermodynamic parameters.


CONCLUSIONS
The presented data demonstrate a proof of principle and confirm that the DSC technique has a potential to monitor changes in the CRC blood plasma proteome. This study is a further step toward the validation of calorimetry as a diagnostic tool.
Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Svetla
S
Todinova
Sashka
S
Krumova
Panayot
P
Kurtev
Valentin
V
Dimitrov
Lachezar
L
Djongov
Zlate
Z
Dudunkov
Stefka G
SG
Taneva
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D016022:Case-Control Studies; D003106:Colon / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000097:blood / Q000145:classification / Q000175:diagnosis*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D010949:Plasma / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D012007:Rectum / Q000378:metabolism; D013816:Thermodynamics
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D020543:Proteome