Concentric zones for pheromone components in the mushroom body calyx of the moth brain.
The spatial distribution of input and output neurons in the mushroom body (MB) calyx was investigated in the silkmoth Bombyx mori. In Lepidoptera, the brain has a specialized system for processing sex pheromones. How individual pheromone components are represented in the MB has not yet been elucidated. Toward this end, we first compared the distribution of the presynaptic boutons of antennal lobe projection neurons (PNs), which transfer odor information from the antennal lobe to the MB calyx. The axons of PNs that innervate pheromonal glomeruli were confined to a relatively small area within the calyx. In contrast, the axons of PNs that innervate nonpheromonal glomeruli were more widely distributed. PN axons for the minor pheromone component covered a larger area than those for the major pheromone component and partially overlapped with those innervating nonpheromonal glomeruli, suggesting the integration of the minor pheromone component with plant odors. Overall, we found that PN axons innervating pheromonal and nonpheromonal glomeruli were organized into concentric zones. We then analyzed the dendritic fields of Kenyon cells (KCs), which receive inputs from PNs. Despite the strong regional localization of axons of different PN classes, the dendrites of KCs were less well classified. Finally, we estimated the connectivity between PNs and KCs and suggest that the dendritic field may be organized to receive different amounts of pheromonal and nonpheromonal inputs. PNs for multiple pheromone components and plant odors enter the calyx in a concentric fashion, and they are read out by the elaborate dendritic field of KCs.
Intelligent Cooperative Systems Laboratory, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo 153-8904, Japan.
Shigehiro
S
Namiki
Mitsuko
M
Takaguchi
Yoichi
Y
Seki
Tomoki
T
Kazawa
Ryota
R
Fukushima
Chika
C
Iwatsuki
Ryohei
R
Kanzaki
D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D002452:Cell Count; D003712:Dendrites / Q000502:physiology; D018613:Microscopy, Confocal; D009036:Moths / Q000033:anatomy & histology*; D024521:Mushroom Bodies / Q000166:cytology* / Q000378:metabolism; D009415:Nerve Net / Q000502:physiology; D009474:Neurons / Q000145:classification / Q000166:cytology / Q000378:metabolism*; D009833:Olfactory Pathways / Q000502:physiology; D010675:Pheromones / Q000378:metabolism*; D010775:Photic Stimulation; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D010675:Pheromones; D005680:gamma-Aminobutyric Acid