FRAP analysis: accounting for bleaching during image capture.
The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approach in FRAP analysis.
Department of Chemical Engineering, University of Florida, Gainesville Florida, United States of America.
Jun
J
Wu
Nandini
N
Shekhar
Pushkar P
PP
Lele
Tanmay P
TP
Lele
D000818:Animals; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D022001:Focal Adhesions / Q000378:metabolism; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008962:Models, Theoretical; D057054:Molecular Imaging / Q000379:methods*; D041681:NIH 3T3 Cells; D038761:Photobleaching
D055767:Immobilized Proteins