Simultaneous determination of lappaconitine hydrobromide and isopropiram fumarate in rabbit plasma by capillary electrophoresis with electrochemiluminescence detection.
A CE electrochemiluminescence (CE-ECL) method for simultaneous determination of lappaconitine hydrobromide (LH) and isopropiram fumarate (IF) has been first established, with a chemically modified platinum electrode by europium (III)-doped Prussian blue analogue film as a working electrode. The conditions for CE separation and ECL detection are discussed and optimized in detail. It has been proved that 20 mmol/L phosphate buffer (pH 8.5) containing 5% (v/v) ACN and 0.17 mol/L SDS could achieve the most favorable resolution, and the high sensitivity of detection was obtained by maintaining the detection potential at 1.23 V. Under optimized conditions, a baseline separation for the two analytes was achieved within 6 min, and the standard curves were linear in the range of 1.0×10(-7) ~ 5.0 × 10(-5) g/mL for LH and 4.0 × 10(-8) ~ 1.0 × 10(-5) g/mL for IF with the detection limits (3σ) of 6.6 × 10(-8) g/mL for LH and 3.7 × 10(-8) g/mL for IF, respectively. The precisions of intra- and interday measurements for LH and IF were less than 4.21 and 2.61%, respectively. The applicability of the proposed method was illustrated in the determination of LH and IF in rabbit plasma with recoveries between 95.6 and 103.0%.
Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, Key Laboratory of Polymer Materials of Gansu Province, Key Laboratory of Eco-Environment-Related Polymer Materials Ministry of Education, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, China. mzhou8367@sina.com
Min
M
Zhou
Yujie
Y
Li
Caiyun
C
Liu
Yongjun
Y
Ma
Juan
J
Mi
Shulun
S
Wang
D000157:Aconitine / Q000031:analogs & derivatives* / Q000097:blood; D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D008163:Luminescent Measurements / Q000379:methods*; D011725:Pyridines / Q000097:blood*; D011817:Rabbits; D015203:Reproducibility of Results
D011725:Pyridines; C002107:propiram; D000157:Aconitine; C022150:lappaconitine