Replacement of H1 linker histone during bovine somatic cell nuclear transfer.
Linker histone variants are involved in regulation of chromosome organization and gene transcription; several subtypes are expressed in the maturing oocyte and developing embryo. In Xenopus and mice, the transition between linker histone variants occurred following nuclear transfer, and apparently contributed to donor nuclear reprogramming. To determine whether such linker histone replacement occurred after bovine nuclear transfer, red fluorescent protein (RFP) tagged H1e (somatic linker histone H1e) donor cells and Venus tagged H1foo eggs were created, enucleated eggs were injected with donor cells, and embryos were created by fusion. Using fluorescence microscopy, release of H1e in the donor nucleus, acquisition of H1foo by donor chromosomes, and the H1foo-to-H1e transition were observed in live cells. Linker histone replacement occurred more slowly in bovine than murine embryos. Low levels of diffuse red fluorescence (H1e) in the donor nucleus were detected 5 h after fusion, at which time green fluorescence (H1foo) had incorporated into donor chromosomes. However, complete replacement did not occur until 8 h after fusion. We concluded that the linker histone transition was sufficiently conserved among species, which provided further evidence regarding its important role in nuclear reprogramming.
College of Veterinary Medicine, Northwest A&F University, Shaanxi Stem Cell Engineering and Technology Center, Yangling 712100, China.
Yan
Y
Yun
Gui-min
GM
Zhao
Su-jun
SJ
Wu
Wei
W
Li
An-min
AM
Lei
D000818:Animals; D002417:Cattle / Q000196:embryology*; D065150:Cellular Reprogramming / Q000502:physiology; D002875:Chromosomes / Q000378:metabolism; D019976:Cloning, Organism / Q000662:veterinary*; D005260:Female; D005347:Fibroblasts / Q000648:ultrastructure; D006657:Histones / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008856:Microscopy, Fluorescence; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D009865:Oocytes / Q000648:ultrastructure; D014162:Transfection
D006657:Histones