Antimicrobial effect of conventional root canal medicaments vs propolis against Enterococcus faecalis, Staphylococcus aureus and Candida albicans.
OBJECTIVE
To evaluate and compare antimicrobial effect of various root canal medicaments against Enterococcus faecalis, Staphylococcus aureus and Candida albicans.


METHODS
Six root canal medicaments: 2% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) Calcium hydroxide (Ca(OH)2), EDTA, MTAD and propolis and three microorganisms: Staphylococcus aureus, Enterococcus faecalis and Candida albicans were used. These strains were inoculated in brain heart infusion (BHI) and incubated at 37 degrees C for 24 hours. For the agar diffusion test (ADT), petri plates with 20 ml of BHI agar were inoculated with 0.1 ml of the microbial suspensions, using sterile swabs that were spread on the medium, obtaining growth injunction. Paper disks were immersed in the experimental solutions for 1 minute. Subsequently, four papers disks containing one of the substances were placed on the BHI agar surface in each agar plate. The plates were incubated at 37°C for 48 hours. The diameter of microbial inhibition was measured around the papers disks containing the substances. One way ANOVA followed by Tukey's post-hoc test were used. p-value >0.05 was considered statistically significant.


RESULTS
Propolis and other irrigants were found to be effective on C. albicans, S. aureus and E. faecalis. CHX and MTAD were found to be most effective amongst all the materials tested followed by propolis.


CONCLUSIONS
Propolis showed antimicrobial activity against E. faecalis, S. aureus, C. albicans. It appears that propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans.


CONCLUSIONS
Propolis is an effective intracanal irrigant in eradicating E. faecalis and C. albicans. It could be used as an alternative intracanal medicament.
Reader, Department of Conservative Dentistry and Endodontics Krishna School of Dental Sciences, KIMSDU, Karad Satara, Maharashtra, India. sandeshphaphe@rediffmail.com
Sudha
S
Mattigatti
P
P
Ratnakar
Shridhar
S
Moturi
Sarath
S
Varma
Surabhi
S
Rairam
D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects*; D013293:Enterococcus faecalis / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D011429:Propolis / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects*
D000891:Anti-Infective Agents, Local; D012387:Root Canal Filling Materials; D011429:Propolis