Novel angiotensin I-converting enzyme inhibitory peptides produced in fermented milk by specific wild Lactococcus lactis strains.
The ability of specific wild Lactococcus lactis strains to hydrolyze milk proteins to release angiotensin I-converting enzyme (ACE) inhibitory peptides was evaluated. The peptide profiles were obtained from the <3 kDa water-soluble extract and subsequently fractionated by reversed-phase HPLC. The fractions with the lowest half-maximal inhibitory concentration estimated values (peptide concentration necessary to inhibit ACE activity by 50%) were Lc. lactis NRRL B-50571 fraction (F)1 (0.034 ± 0.002 μg/mL; mean ± SD) and Lc. lactis NRRL B-50572B F 0005 (0.041 ± 0.003 μg/mL; mean ± SD). All peptide fractions were analyzed by reversed-phase HPLC tandem mass spectrometry. Twenty-one novel peptide sequences associated with ACE inhibitory (ACEI) activity were identified. Several novel ACEI peptides presented peptides encrypted with proven hypotensive activity. In conclusion, specific wild Lc. lactis strains were able to hydrolyze milk proteins to generate potent ACEI peptides. However, further studies are necessary to find out the relationship between Lc. lactis strain proteolytic systems and their ability to biogenerate hypotensive peptides.
Laboratorio de Química y Biotecnología de Productos Lácteos Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD), Carretera a La Victoria Km. 0.6 Hermosillo, Sonora 83304, Mexico.
J C
JC
Rodríguez-Figueroa
A F
AF
González-Córdova
M J
MJ
Torres-Llanez
H S
HS
Garcia
B
B
Vallejo-Cordoba
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D005285:Fermentation; D013294:Lactococcus lactis / Q000378:metabolism*; D008892:Milk / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology*; D010455:Peptides / Q000032:analysis*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods
D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D007703:Peptidyl-Dipeptidase A