Fabrication of novel hierarchical structured Fe₃O₄ @LnPO₄ (Ln=Eu, Tb, Er) multifunctional microspheres for capturing and labeling phosphopeptides.
Novel core-shell structured Fe3O4@LnPO4 (Ln=Eu, Tb, Er) multifunctional microspheres with a magnetic Fe3O4 core and a LnPO4 shell covered with spikes are synthesized for the first time through the combination of a homogeneous precipitation approach and an ion-exchange process. Their potential for selective capture, rapid separation, and easy mass spectrometry (MS) labeling of the phosphopeptides from complex proteolytic digests are evaluated. These affinity microspheres can improve the specificity for capture of the phosphopeptides, realize fast magnetic separation, enhance the MS detection signals, and directly identify phosphopeptides through 80 Da mass loss in the mass spectra. The synthesis strategy could become a general and effective technique for similar core-shell hierarchical structures.
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Changchun 130022, PR China; Graduate School of the Chinese Academy of Sciences, Beijing 100049, PR China.
Zhi-Gang
ZG
Wang
Gong
G
Cheng
Yan-Lin
YL
Liu
Ji-Lin
JL
Zhang
De-Hui
DH
Sun
Jia-Zuan
JZ
Ni
D004871:Erbium / Q000737:chemistry; D005063:Europium / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D007477:Ions; D008280:Magnetics; D013058:Mass Spectrometry / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D036103:Nanotechnology / Q000379:methods*; D010710:Phosphates / Q000737:chemistry*; D010748:Phosphopeptides / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013725:Terbium / Q000737:chemistry; D014961:X-Ray Diffraction
D007477:Ions; D010710:Phosphates; D010748:Phosphopeptides; D013725:Terbium; D005063:Europium; D004871:Erbium; D052203:Ferrosoferric Oxide