[Cloning and bioinformatics analysis of PFC0460w in Plasmodium falciparum].
The gene fragment of PFC0460w was amplified from RNA of Plasmodium falciparum 3D7 strain with RT-PCR, and cloned into pGEM-T easy vector. The recombinant plasmid was transformed into E. coli DH5alpha and the positive clones were selected, which were identified by PCR and sequencing. The results showed that there were three sequences of PFC0460w fragment, respectively with length of 618, 597 and 543 bp. The 618 bp fragment was completely consistent with the sequence published in PlasmoDB (GenBank No. XM_001351147), and the 597 bp and 543 bp fragments were submitted to GenBank with Accession No. of JF799872 and JF799873, respectively. 205 amino acids were encoded by the 618 bp fragment, and five kinds of protein structure were predicted by Robetta.
Innovation Laboratory, Medical School of Huzhou Teachers College, Huzhou 313000, China. zhouhc529@hutc.zj.cn
Hong-chang
HC
Zhou
Jia-li
JL
Dai
Cheng-cheng
CC
Fan
Hui-ya
HY
Zhong
Yu-qing
YQ
Shen
D000818:Animals; D003001:Cloning, Molecular; D019295:Computational Biology; D017125:Genes, Protozoan; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D010963:Plasmodium falciparum / Q000235:genetics*