Bioactivity and mechanical properties of collagen composite membranes reinforced by chitosan and β-tricalcium phosphate.
In this study, we analyzed the effects of varying concentrations of chitosan (CS) and β-tricalcium phosphate [β-TCP, Ca(3)(PO(4))(2)] on the mechanical and cell-adhesion properties of a collagen (CG) matrix for use in guided bone regeneration (GBR). Three different CS concentrations (0.5, 1, and 2%) and five different contents of β-TCP (0, 17, 29, 38, and 44%) were investigated. The composite membranes were analyzed by scanning electron microscopy and cell-adhesion, flexural-strength, and tear-strength assays. The results show that the cell-adhesion and mechanical properties of the composite membranes improved with increasing β-TCP and CS contents, yielding suitable levels of the adhesion of cells and adequate mechanical stability to ensure successful GBR. The CS adhered to the microsized β-TCP, which was distributed uniformly in the composite membranes. The β-TCP and CS have no negative effect on the cell morphology, viability, and proliferation and possess good biocompatibility. This study demonstrates that β-TCP/CS/CG composite membranes are good candidates for GBR membranes in future applications.
Department of Dental Biomaterials and Bioengineering, College of Dentistry, Yonsei University, Seoul 120-752, Republic of Korea.
Sang-Bae
SB
Lee
Jae-Sung
JS
Kwon
Yong-Keun
YK
Lee
Kwang-Mahn
KM
Kim
Kyoung-Nam
KN
Kim
D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*
D002130:Calcium Phosphates; D008567:Membranes, Artificial; C485817:beta-tricalcium phosphate; D003094:Collagen; D048271:Chitosan