Microwave-assisted method for microassay of proteins using bicinchoninic acid.
A microwave-assisted microassay has been developed for estimation of proteins, using bicinchoninic acid (BCA). The protocol described here is rapid with a reaction time of merely 95 sec, requires lesser amounts of BCA and other reagents, and tolerates larger or comparable concentration of various interfering substances.
Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110016, India.
Sulakshana
S
Jain
Shweta
S
Sharma
Munishwar N
MN
Gupta
D002623:Chemistry Techniques, Analytical; D003124:Colorimetry / Q000592:standards*; D004305:Dose-Response Relationship, Drug; D008872:Microwaves; D017830:Octoxynol / Q000494:pharmacology; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D011804:Quinolines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods / Q000592:standards; D013997:Time Factors
D011506:Proteins; D011804:Quinolines; D017830:Octoxynol; C047117:bicinchoninic acid