Expression of pendrin and periostin in allergic rhinitis and chronic  rhinosinusitis.
BACKGROUND
Pendrin and periostin are newly identified mediators of the inflammatory process. The expression of these proteins in human sinonasal tissue and their roles in allergic rhinitis and chronic rhinosinusitis remain to be elucidated. This study investigated the expression of pendrin and periostin in sinonasal tissue of patients with allergic rhinitis, chronic rhinosinusitis, and aspirin-induced asthma. Prospective control study conducted at Yamagata University, Japan.


METHODS
Surgical samples were investigated by means of real-time reverse transcription-polymerase chain reaction to evaluate the expression of pendrin and periostin mRNA. The presence and location of pendrin and periostin were determined by immunohistochemistry and Western blotting.


RESULTS
Pendrin and periostin production was significantly higher in patients with nasal disorders than in controls. Further significant increases in periostin expression were noted in patients with chronic rhinosinusitis with nasal polyps and in those with aspirin-induced asthma. Immunohistochemistry revealed positive staining for pendrin in epithelial cells and submucosal glands and for periostin in the basement membrane in all three disorders, and additionally for periostin in nasal polyp tissue in chronic rhinosinusitis and aspirin-induced asthma.


CONCLUSIONS
Production of pendrin and periostin is upregulated in allergic rhinitis, chronic rhinosinusitis with nasal polyps, and aspirin-induced asthma. These findings suggest that pendrin can induce mucus production and that periostin can induce tissue fibrosis and remodeling in the nasal mucosa. Therefore, these mediators may be therapeutic target candidates for allergic rhinitis, chronic rhinosinusitis with nasal polyps, and aspirin-induced asthma.
Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, Yamagata University, Japan.
Akihiro
A
Ishida
Nobuo
N
Ohta
Yusuke
Y
Suzuki
Seiji
S
Kakehata
Kimihiro
K
Okubo
Hiroki
H
Ikeda
Hiroshi
H
Shiraishi
Kenji
K
Izuhara
D000328:Adult; D000368:Aged; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002908:Chronic Disease; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012220:Rhinitis / Q000235:genetics / Q000378:metabolism*; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000235:genetics / Q000378:metabolism*; D012852:Sinusitis / Q000235:genetics / Q000378:metabolism*; D000076988:Sulfate Transporters; D055815:Young Adult
D015815:Cell Adhesion Molecules; D026901:Membrane Transport Proteins; C512835:POSTN protein, human; D012333:RNA, Messenger; C085609:SLC26A4 protein, human; D000076988:Sulfate Transporters