Polydimethylsiloxane embedded mouse aorta ex vivo perfusion model: proof-of-concept study focusing on atherosclerosis.
Existing mouse artery ex vivo perfusion models have utilized arteries such as carotid, uterine, and mesenteric arteries, but not the aorta. However, the aorta is the principal vessel analyzed for atherosclerosis studies in vivo. We have devised a mouse aorta ex vivo perfusion model that can bridge this gap. Aortas from apoE((-/-)) mice are embedded in a transparent, gas-permeable, and elastic polymer matrix [polydimethylsiloxane (PDMS)] and artificially perfused with cell culture medium under cell culture conditions. After 24 h of artificial ex vivo perfusion, no evidence of cellular apoptosis is detected. Utilizing a standard confocal microscope, it is possible to image specific receptor targeting of cells in atherosclerotic plaques during 24 h. Imaging motion artifacts are minimal due to the polymer matrix embedding. Re-embedding of the aorta enables tissue sectioning and immuno-histochemical analysis. The ex vivo data are validated by comparison with in vivo experiments. This model can save animal lives via production of multiple endpoints in a single experiment, is easy to apply, and enables straightforward comparability with pre-existing atherosclerosis in vivo data. It is suited to investigate atherosclerotic disease in particular and vascular biology in general.
University Hospital Basel, Nanomedicine Group, Clinic for Intensive Care, Petersgraben 4, CH-4031 Basel, Switzerland. wangxu@uhbs.ch
Xueya
X
Wang
Marc P
MP
Wolf
Rahel Bänziger
RB
Keel
Roman
R
Lehner
Patrick R
PR
Hunziker
D000818:Animals; D001011:Aorta / Q000473:pathology*; D001018:Aortic Diseases / Q000473:pathology*; D050197:Atherosclerosis / Q000473:pathology*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004195:Disease Models, Animal; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008853:Microscopy / Q000295:instrumentation; D009924:Organ Culture Techniques / Q000295:instrumentation / Q000379:methods; D010477:Perfusion / Q000295:instrumentation*; D010865:Pilot Projects; D016610:Tissue Embedding / Q000295:instrumentation*
D004129:Dimethylpolysiloxanes; C013830:baysilon