Platelet aggregation abnormalities in patients with fetal losses: the GP6 gene polymorphism.
OBJECTIVE
To evaluate the GP6 gene polymorphism in patients with sticky platelet syndrome (SPS) and fetal loss.


METHODS
Genetic association study.


METHODS
Perinatal center.


METHODS
Twenty-seven patients with SPS, manifested as fetal loss, and 42 control subjects without SPS and no history of fetal loss and thrombosis.


METHODS
SPS was diagnosed by platelet aggregometry (PACKS-4 aggregometer; Helena Laboratories). Seven single-nucleotide polymorphisms (SNPs) of the GP6 gene were evaluated.


METHODS
Occurrence of SNPs of the GP6 gene in SPS patients versus control subjects.


RESULTS
We found a higher occurrence of three SNPs of the GP6 gene in SPS patients versus control subjects (rs1671153: 0.204 vs. 0.048, odds ratio [OR] 5.116, 95% confidence interval [CI] 1.536-17.03; rs1654419: 0.204 vs. 0.071, OR 3.326, 95% CI 1.149-9.619; rs1613662: 0.204 vs. 0.071, OR 3.326, 95% CI 1.149-9.619). The haplotype analysis showed a significantly higher occurrence of two haplotypes (CTGAG in haplotype 5: 0.185 vs. 0.059, OR 3.568, 95% CI 1.142-11.14; and CGATAG in haplotype 6: 0.204 vs. 0.048, OR 4.961, 95% CI 1.488-16.53).


CONCLUSIONS
Our results, especially the higher occurrence of haplotypes CTGAG and CGATAG in SPS patients, support the idea that GP6 gene polymorphism may be associated with platelet hyperaggregability, a possible cause of fetal loss.
Department of Hematology and Transfusion Medicine, National Center of Hemostasis and Thrombosis, Jessenius Faculty of Medicine, Comenius University, Martin, Slovakia.
Juraj
J
Sokol
Kamil
K
Biringer
Maria
M
Skerenova
Miroslav
M
Hasko
Lenka
L
Bartosova
Jan
J
Stasko
Jan
J
Danko
Peter
P
Kubisz
D000026:Abortion, Habitual / Q000097:blood / Q000235:genetics; D000022:Abortion, Spontaneous / Q000097:blood* / Q000235:genetics*; D000328:Adult; D001791:Blood Platelet Disorders / Q000097:blood* / Q000235:genetics*; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005865:Gestational Age; D006239:Haplotypes; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D010974:Platelet Aggregation / Q000235:genetics*; D010979:Platelet Function Tests; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D018570:Risk Assessment; D012307:Risk Factors; D018154:Slovakia; D055815:Young Adult
D010980:Platelet Membrane Glycoproteins; C098341:platelet membrane glycoprotein VI