The protein composition of the digestive fluid from the venus flytrap sheds light on prey digestion mechanisms.
The Venus flytrap (Dionaea muscipula) is one of the most well-known carnivorous plants because of its unique ability to capture small animals, usually insects or spiders, through a unique snap-trapping mechanism. The animals are subsequently killed and digested so that the plants can assimilate nutrients, as they grow in mineral-deficient soils. We deep sequenced the cDNA from Dionaea traps to obtain transcript libraries, which were used in the mass spectrometry-based identification of the proteins secreted during digestion. The identified proteins consisted of peroxidases, nucleases, phosphatases, phospholipases, a glucanase, chitinases, and proteolytic enzymes, including four cysteine proteases, two aspartic proteases, and a serine carboxypeptidase. The majority of the most abundant proteins were categorized as pathogenesis-related proteins, suggesting that the plant's digestive system evolved from defense-related processes. This in-depth characterization of a highly specialized secreted fluid from a carnivorous plant provides new information about the plant's prey digestion mechanism and the evolutionary processes driving its defense pathways and nutrient acquisition.
Max Planck Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Potsdam, Germany.
Waltraud X
WX
Schulze
Kristian W
KW
Sanggaard
Ines
I
Kreuzer
Anders D
AD
Knudsen
Felix
F
Bemm
Ida B
IB
Thøgersen
Andrea
A
Bräutigam
Line R
LR
Thomsen
Simon
S
Schliesky
Thomas F
TF
Dyrlund
Maria
M
Escalante-Perez
Dirk
D
Becker
Jörg
J
Schultz
Henrik
H
Karring
Andreas
A
Weber
Peter
P
Højrup
Rainer
R
Hedrich
Jan J
JJ
Enghild
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D031257:Droseraceae / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D007313:Insecta / Q000378:metabolism*; D008969:Molecular Sequence Data; D053147:Plant Exudates / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D059748:Proteolysis; D016415:Sequence Alignment; D059467:Transcriptome
D018076:DNA, Complementary; D053147:Plant Exudates; D010940:Plant Proteins