[The coastal areas of Yantai human and swine hepatitis E virus genotyping analysis].
OBJECTIVE
Survey of the coastal city of Yantai, from human and swine hepatitis E virus (HEV) genotype correlation.


METHODS
Application of reverse transcription nested polymerase chain reaction (RT-nPCR) method for local acute sporadic hepatitis E patients,normal population of HEV-IgM positive and local pig farm pigs were HEV RNA detection. And HEV RNA positive samples for cloning sequencing and sequence analysis.


RESULTS
In 16 patients with acute sporadic hepatitis E in 7 cases of RNA positive stool specimens of HEV; 51 IgM positive sera of normal people in specimens with 1 HEV RNA positive; 34 pig bile specimens with 1 HEV RNA positive. Sequence analysis revealed the region HEV strains and swine strains in the ORF2 region of nucleotide sequence homology is 87%-98.1%. 7 strains of hepatitis E virus genotype in patients and 1 strains of swine hepatitis E virus genotypes are type IV, gene sequence homology between the 87%-98.1%; there were 6 patients and porcine gene sequence homology in 93.9%-98.1% between,for type a subtype; 1 patients and porcine gene sequence homology in 87%, for the type D subtype. Normal population of 1 cases of hepatitis E virus genotype for I type D subtype. Human and porcine HEV ORF2 gene fragment and HEV part I-IV representative strains were compared, and the nucleotide sequence homology were 82.5%-100%, 81.7%-92.9%, 81.4%-93.9%, 84.9%-100%.


CONCLUSIONS
The area population prevalence of HEV in the presence of 2 genotype 3 subtype genes, mainly to IV A, in pigs with popular HEV gene with a high homology; HEV type I in the crowd disperses in the presence of.
Yantai Municipal Center for Disease Control and Prevention, Shandong 264003, China.
Lian-Feng
LF
Gong
Juan
J
Liu
Wen-Qing
WQ
Han
Wei-Hong
WH
Cui
Zhen-Lu
ZL
Sun
Mei
M
Jiang
D000818:Animals; D005838:Genotype; D016751:Hepatitis E / Q000175:diagnosis / Q000453:epidemiology; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics*; D006801:Humans; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis; D017422:Sequence Analysis, DNA; D013552:Swine
D012367:RNA, Viral