Single nucleotide polymorphism and production of IL-1β and IL-10 cytokines in febrile seizures.
Inflammation and genetics may play a role in the pathogenesis of febrile seizures. The aim of this study was to investigate the spontaneous and lipopolysaccharide (LPS)-induced production of IL-1β and IL-10, and the association between IL-1β (-511) and IL-10 (-1082) single nucleotide polymorphisms with LPS-induced cytokine production. The study included 92 febrile seizure patients and 132 healthy controls. First, we isolated genomic DNA and by using PCR-RFLP we genotyped the individuals for the cytokines gene polymorphism. Second, peripheral mononuclear cells of the individuals were isolated and stimulated with LPS to measure secretion capacity of IL-1β and IL-10 using specific ELISA kits. We found that both the IL-1β and IL-10 production was increased in febrile seizures. The rapid increase of IL-1β production in the supernatants of the LPS-induced cells was significantly higher at the fourth and the twenty-fourth hours in febrile and complex febrile seizures, respectively. The distribution of IL-10 (-1082) G allele differs significantly between cases and controls. The IL-1β (-511) G/A and the IL-10 (-1082) G/A genotype combination was found to be higher in patients with febrile seizure. Our results showed that IL-1β and IL-10 production was not influenced by the single nucleotide polymorphisms in the pathogenesis of febrile seizures.
Division of Pediatric Genetics, Department of Pediatrics, Akdeniz University School of Medicine, Antalya, Turkey.
B G
BG
Nur
D
D
Sahinturk
M
M
Coskun
O
O
Duman
U
U
Yavuzer
S
S
Haspolat
D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000378:metabolism; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000235:genetics*; D053583:Interleukin-1beta / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011134:Polysaccharides / Q000494:pharmacology; D003294:Seizures, Febrile / Q000235:genetics* / Q000473:pathology; D013997:Time Factors
D016207:Cytokines; D053583:Interleukin-1beta; D011134:Polysaccharides; D016753:Interleukin-10