Establishment of Eimeria tenella (local isolate) in chicken embryos.
Development of an in vitro Eimeria (E.) tenella model could be valuable as a tool for vaccine, coccidiostats or molecular biology research. 1.0 × 10,000 sporozoites per 0.1 mL were inoculated into the allantoic cavity of ten-day-old chicken embryos. The complete life-cycle of E. tenella was accomplished in eight-nine days at 37 °C and 70% humidity. The addition of 100 U insulin to the embryos could remarkably improve the output of oocysts. The development of the parasite within the embryos was systematically observed, allowing guidelines to be set regarding the appropriate times at which different developmental stages of the parasite may be sampled.
Key Laboratory for Animal Parasitology, Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518 Ziyue Road, Minhang District, Shanghai 200241, China.
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L
Jiang
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Q
Zhao
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S
Zhu
H
H
Han
H
H
Dong
B
B
Huang
D000482:Allantois / Q000469:parasitology; D000818:Animals; D002642:Chick Embryo / Q000469:parasitology*; D002645:Chickens; D049033:Chorioallantoic Membrane / Q000469:parasitology; D016786:Eimeria tenella / Q000187:drug effects / Q000254:growth & development*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D033661:Oocysts / Q000254:growth & development; D034101:Sporozoites / Q000254:growth & development; D013997:Time Factors
D007004:Hypoglycemic Agents; D007328:Insulin