Corylucinine, a new alkaloid from Corydalis cava (Fumariaceae), and its cholinesterase activity.
A new benzyldihydroisoquinoline alkaloid (1) was isolated from the tubers of Corydalis cava and named corylucinine. Additionally, 8-trichloromethyl-7,8-dihydropalmatine (2), an isolation artifact of tetrahydropalmatine, was obtained. The structures were established by spectroscopic (including 2D NMR and optical rotation) and HR-ESI-MS methods. Both compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibitory activity. In comparison with the used standards, both compounds showed only moderate inhibitory activity against HuAChE (IC50,. HuAChE = 127.6 +/- 5.2 microM for 1, and IC50, HuAChE = 82.9 +/- 3.9 microM for 2) and none against HuBuChE.
Department of Inorganic and Organic Chemistry, Faculty of Pharmacy, Charles University, , 500 05 Hradec Králové, Czech Republic.
Zdenĕk
Z
Novák
Jakub
J
Chlebek
Lubomír
L
Opletal
Pavel
P
Jiros
Katerina
K
Macáková
Jiri
J
Kunes
Lucie
L
Cahlíková
D000110:Acetylcholinesterase / Q000378:metabolism; D000470:Alkaloids / Q000737:chemistry* / Q000494:pharmacology*; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D002802:Cholinesterases / Q000378:metabolism*; D031311:Corydalis / Q000737:chemistry*; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans
D000470:Alkaloids; D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases