An  oleosin-fusion protein driven by the CaMV35S promoter is accumulated in Arabidopsis (Brassicaceae) seeds and correctly targeted to oil bodies.
Oleosin-fusion  technology is used to express desired proteins. It was developed based on the  properties of oleosin; the heterologous protein gene is fused to the oleosin gene and the fusion gene is driven by a seed-specific promoter. We replaced  the seed specific promoter with the CaMV35S promoter to dive a gfp-oleosin fusion  gene in transformed Arabidopsis. The heterologous oleosin-fusion protein  was mainly accumulated in the transgenic Arabidopsis seeds and correctly  targeted to oil bodies. This provides an alternate choice of promoter in  oleosin-fusion technology.
State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai, China.
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D017360:Arabidopsis / Q000196:embryology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D017796:Caulimovirus / Q000235:genetics*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D015388:Organelles / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*
D029681:Arabidopsis Proteins; D011993:Recombinant Fusion Proteins; C079353:oleosin protein, Arabidopsis; D049452:Green Fluorescent Proteins