A nano-sized PARACEST-fluorescence imaging contrast agent facilitates and validates in vivo CEST MRI detection of glioma.
OBJECTIVE
The authors have investigated the usefulness of in vivo chemical exchange saturation transfer MRI for detecting gliomas using a dual-modality imaging contrast agent.


METHODS
A paramagnetic chemical exchange saturation transfer MRI contrast agent, Eu-1,4,7,10-tetraazacclododecane-1,4,7,10-tetraacetic acid-Gly(4) and a fluorescent agent, DyLight 680, were conjugated to a generation 5 polyamidoamine dendrimer to create the dual-modality, nano-sized imaging contrast agent.


RESULTS
The agent was detected with in vivo chemical exchange saturation transfer MRI in an U87 glioma model. These results were validated using in vivo and ex vivo fluorescence imaging.


CONCLUSIONS
This study demonstrated the merits of using a nano-sized imaging contrast agent for detecting gliomas and using a dual-modality agent for detecting gliomas at different spatial scales.
Henry Ford Hospital, Detroit, MI 48202, USA. mesera@rad.hfh.edu
Meser M
MM
Ali
Mohammed Pi
MP
Bhuiyan
Branislava
B
Janic
Nadimpalli Rs
NR
Varma
Tom
T
Mikkelsen
James R
JR
Ewing
Robert A
RA
Knight
Mark D
MD
Pagel
Ali S
AS
Arbab
D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005910:Glioma / Q000175:diagnosis*; D006801:Humans; D008279:Magnetic Resonance Imaging; D061848:Optical Imaging; D011073:Polyamines / Q000737:chemistry; D051381:Rats; D011923:Rats, Nude
D003287:Contrast Media; D050091:Dendrimers; D005456:Fluorescent Dyes; C531249:Poly(amidoamine); D011073:Polyamines