Variants in DENND1A and LHCGR are associated with endometrioid adenocarcinoma.
OBJECTIVE
The aim of this study was to explore the polycystic ovary syndrome (PCOS) related single nucleotide polymorphisms (SNPs) rs13405728 (in gene LHCGR), rs13429458 (in gene THADA) and rs2479106 (in gene DENND1A) in women with endometrial carcinoma.


METHODS
We conducted a case-control study comprising 96 Han Chinese women with endometrial carcinoma, and 192 healthy controls. SNPs rs13405728, rs13429458 and rs2479106 were genotyped by polymerase chain reaction (PCR) and direct sequencing. The effects of body mass index (BMI) and age were evaluated using an unconditional logistic regression model adjusted for potential confounders.


RESULTS
The allele frequencies of SNPs rs2479106 and rs13405728 were significantly different (P<0.05) between endometrial carcinoma group and control group, and the difference was especially significant in the subgroup of endometrioid adenocarcinoma. Genotyping analysis showed that allele G in rs2479106 and allele A in rs13405728 could confer risk to endometrioid adenocarcinoma.


CONCLUSIONS
Our results suggest that SNPs rs2479106 in gene DENND1A and rs13405728 in gene LHCGR are associated with endometrioid adenocarcinoma.
Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, 250021, China.
Zhenyan
Z
Wang
Tao
T
Li
Wei
W
Zhang
Li
L
You
Yueran
Y
Zhao
Mingdi
M
Xia
Han
H
Zhao
Zi-Jiang
ZJ
Chen
D000367:Age Factors; D015992:Body Mass Index; D018269:Carcinoma, Endometrioid / Q000209:etiology / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D053418:Death Domain Receptor Signaling Adaptor Proteins / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000209:etiology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers; D060005:Genotyping Techniques; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D016015:Logistic Models; D008875:Middle Aged; D011085:Polycystic Ovary Syndrome / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011974:Receptors, LH / Q000235:genetics*; D012307:Risk Factors; D017422:Sequence Analysis, DNA
C571984:DENND1A protein, human; D053418:Death Domain Receptor Signaling Adaptor Proteins; D005819:Genetic Markers; D020662:Guanine Nucleotide Exchange Factors; D011974:Receptors, LH