Technical considerations for functional sequencing assays.
Next-generation sequencing technologies need careful design of experiments and evaluation of results to meet field requirements. Here we discuss technical considerations for these high-throughput assays, together with criteria to assess the quality of the results and the necessary validation.
Department of Developmental and Cell Biology, University of California, Irvine, California, USA.
Weihua
W
Zeng
Ali
A
Mortazavi
D000818:Animals; D004247:DNA / Q000032:analysis*; D020869:Gene Expression Profiling / Q000379:methods*; D060005:Genotyping Techniques / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D012313:RNA / Q000032:analysis*
D012313:RNA; D004247:DNA