Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model.
OBJECTIVE
The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation


METHODS
Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured.


RESULTS
When perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols.


CONCLUSIONS
Protocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation.
Department of Reproductive Medicine, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan, China, 250000.
Jian-Min
JM
Zhang
Ying-Chun
YC
Zhang
Li-Hong
LH
Ruan
Heng-Cai
HC
Wang
D000818:Animals; D002417:Cattle; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D005260:Female; D006801:Humans; D023421:Models, Animal; D009926:Organ Preservation / Q000379:methods*; D006080:Ovarian Follicle / Q000502:physiology / Q000648:ultrastructure; D010053:Ovary / Q000166:cytology / Q000502:physiology*; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*
D003451:Cryoprotective Agents