Inhibitory effects of baicalin on orthotopic xenografts of colorectal cancer cells that are deficient in a mismatch repair gene in nude mice.
OBJECTIVE
The aim of this article is to study the inhibitory effects of baicalin on the growth and metastasis of orthotopic xenografts consisting of human HCT-116 colorectal cancer cells that are deficient in the mismatch repair gene hMLH1 in nude mice.


METHODS
A fluorescent orthotopic transplantation model of HCT-116 cells was established. The treatment groups were administered baicalin 50 mg/kg (G2), 100 mg/kg (G3), and 200 mg/kg (G4), and the negative control group (G1) was administered 5 % NaHCO₃. The volume and vascular density of the primary tumors, body weights, survival conditions, and death rates of the mice were analyzed.


RESULTS
On the 14th, 21st, and 28th days, tumor volume in the treatment groups was significantly smaller than that in the control group, and the differences were statistically significant. At the end of the experiment, the survival rate of the experimental animals in the G3 was significantly higher than that in the G1 and G4 (P < 0.05). There were no significant differences in both the weights and surface vascular densities of the primary tumor and the metastatic tumor among all groups.


CONCLUSIONS
Baicalin had a significant inhibitory effect on the growth of nude mouse orthotopic xenografts consisting of human HCT-116 colorectal tumor cells that are deficient in the mismatch repair gene hMLH1.
Nanjing University of Traditional Chinese Medicine Hospital, 210029, Nanjing, China. blyang1971@163.com
Bo-Lin
BL
Yang
Hong-Jin
HJ
Chen
Yu-Gen
YG
Chen
Yun-Fei
YF
Gu
Shu-Peng
SP
Zhang
Qiu
Q
Lin
Yu
Y
Sun
D048868:Adaptor Proteins, Signal Transducing / Q000172:deficiency* / Q000235:genetics; D000818:Animals; D015179:Colorectal Neoplasms / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology; D053843:DNA Mismatch Repair / Q000235:genetics*; D005419:Flavonoids / Q000627:therapeutic use*; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D000070957:MutL Protein Homolog 1; D009362:Neoplasm Metastasis; D009687:Nuclear Proteins / Q000172:deficiency* / Q000235:genetics; D016019:Survival Analysis; D016896:Treatment Outcome; D047368:Tumor Burden; D023041:Xenograft Model Antitumor Assays
D048868:Adaptor Proteins, Signal Transducing; D005419:Flavonoids; C501486:MLH1 protein, human; D009687:Nuclear Proteins; D049452:Green Fluorescent Proteins; C038044:baicalin; D000070957:MutL Protein Homolog 1