OsELF3-1, an ortholog of Arabidopsis early flowering 3, regulates rice circadian rhythm and photoperiodic flowering.
Arabidopsis thaliana early flowering 3 (ELF3) as a zeitnehmer (time taker) is responsible for generation of circadian rhythm and regulation of photoperiodic flowering. There are two orthologs (OsELF3-1 and OsELF3-2) of ELF3 in rice (Oryza sativa), but their roles have not yet been fully identified. Here, we performed a functional characterization of OsELF3-1 and revealed it plays a more predominant role than OsELF3-2 in rice heading. Our results suggest OsELF3-1 can affect rice circadian systems via positive regulation of OsLHY expression and negative regulation of OsPRR1, OsPRR37, OsPRR73 and OsPRR95 expression. In addition, OsELF3-1 is involved in blue light signaling by activating early heading date 1 (Ehd1) expression to promote rice flowering under short-day (SD) conditions. Moreover, OsELF3-1 suppresses a flowering repressor grain number, plant height and heading date 7 (Ghd7) to indirectly accelerate flowering under long-day (LD) conditions. Taken together, our results indicate OsELF3-1 is essential for circadian regulation and photoperiodic flowering in rice.
Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Junming
J
Zhao
Xi
X
Huang
Xinhao
X
Ouyang
Weilan
W
Chen
Anping
A
Du
Ling
L
Zhu
Shiguang
S
Wang
Xing Wang
XW
Deng
Shigui
S
Li
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics; D002940:Circadian Rhythm / Q000235:genetics*; D056925:Circadian Rhythm Signaling Peptides and Proteins / Q000235:genetics*; D035264:Flowers / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D008027:Light; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D017440:Photoperiod; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics / Q000528:radiation effects; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics
D029681:Arabidopsis Proteins; D056925:Circadian Rhythm Signaling Peptides and Proteins; D010940:Plant Proteins; D020033:Protein Isoforms; D014157:Transcription Factors; C432430:At2g25930 protein, Arabidopsis