Analysis of free nucleotide pools of mouse liver tissue by high-pressure liquid chromatography (HPLC).
In this paper, analysis of free nucleotides from mouseliver tissue during different day times has been described. Perchloric acid extract of mouse liver tissue was neutralized with tri-N-octylamine in trichlorotriflouroethane and after removal of ClO4(-), subjected to preliminary purification on a Cu(2+)-loaded column of Chelex 100. A high-pressure liquid chromatographic (HPLC) anion-exchange procedure used in the study gave a good resolution of free nucleotides on a single column.
Department of Biology, Faculty of Science, Payame Noor University, Nakhel St. Lashkarak Rd, Tehran, 19569, Iran. hosseini@pnu.ac.ir
Reza Haji Hosseini Baghdad
RH
Abadi
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D051379:Mice; D009711:Nucleotides / Q000032:analysis* / Q000302:isolation & purification; D013997:Time Factors
D009711:Nucleotides