Mucus digestion improves the detection of Chlamydia trachomatis and Neisseria gonorrhoeae on the cobas 4800.
We compared the performance of cobas 4800 PCR for Neisseria gonorrhoeae with culture and found an association between pipetting errors and positive culture, caused by mucopurulent discharge. The problem may be resolved by pretreating samples with Sputasol, thus improving detection of both Chlamydia trachomatis and Neisseria gonorrhoeae.
From Aotea Pathology Ltd, Wellington, New Zealand.
Amanda
A
Miller
Collette
C
Bromhead
Mark
M
Jones
Paul
P
Tustin
D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005784:Gene Amplification; D006069:Gonorrhea / Q000175:diagnosis*; D006801:Humans; D008297:Male; D009093:Mucus; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D009520:New Zealand; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D011933:Reagent Kits, Diagnostic