Assessment of interobserver variability in mitotic figure counting in different histological grades of oral squamous cell carcinoma.
Mitotic counting is often used for classification, grading and prognosis of tumors. The count usually stands as a decision point for treatment as well. The easiest way of counting the number of mitoses is done by screening routine H&E stained slides. However, for proper mitotic counting, certain strict protocols should be taken into consideration. This study on 30 cases of different grades of oral squamous cell carcinoma was undertaken to determine the interobserver variations in two different groups: Group1 (A1, A2), who were given certain criteria to be followed during the counting of the mitotic figures and group 2 investigators (B1, B2) who were unaware of such criteria. The paired t-test gives a correlation of 0.988 and a significant difference of 0.000 between the two investigators in group 1. The correlation was 0.650 with a significant difference of 0.058 between two investigators in-group 2, indicating that group 1 observers exhibit good interobserver agreement. The results emphasize that following of strict protocols are of great help in determining the accuracy of mitotic counting.
Department of Oral and Maxillofacial Surgery Lenora Dental College, Rajahmundry, Andhra Pradesh, India.
K Shailaja
KS
Yadav
Sudhir
S
Gonuguntla
Kranti Kiran Reddy
KK
Ealla
Surekha Reddy
SR
Velidandla
C R Charan
CR
Reddy
M D
MD
Prasanna
Sanjay Reddy
SR
Bommu
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D008940:Mitotic Index; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology*; D060787:Neoplasm Grading; D015588:Observer Variation; D015203:Reproducibility of Results