Label-free subcellular 3D live imaging of preimplantation mouse embryos with full-field optical coherence tomography.
Early patterning and polarity is of fundamental interest in preimplantation embryonic development. Label-free subcellular 3D live imaging is very helpful to its related studies. We have developed a novel system of full-field optical coherence tomography (FF-OCT) for noninvasive 3D subcellular live imaging of preimplantation mouse embryos with no need of dye labeling. 3D digitized embryos can be obtained by image processing. Label-free 3D live imaging is demonstrated for the mouse embryos at various typical preimplantation stages with a spatial resolution of 0.7 [micro sign]m and imaging rate of 24 fps. Factors that relate to early patterning and polarity, such as pronuclei in zygote, shapes of zona pellucida, location of second polar body, cleavage planes, and the blastocyst axis, can be quantitatively measured. The angle between the two second cleavage planes is accurately measured to be 87 deg. It is shown that FF-OCT provides a potential breakthrough for early patterning, polarity formation, and many other preimplantation-related studies in mammalian developmental biology.
Tsinghua University, Department of Physics and State Key Lab of Low-Dimensional Quantum Physics, Beijing, China.
Jing-gao
JG
Zheng
Danyu
D
Lu
Tianyuan
T
Chen
Chengming
C
Wang
Ning
N
Tian
Fengying
F
Zhao
Tiancheng
T
Huo
Ning
N
Zhang
Dieyan
D
Chen
Wanyun
W
Ma
Jia-lin
JL
Sun
Ping
P
Xue
D000818:Animals; D001755:Blastocyst / Q000648:ultrastructure*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008813:Mice, Inbred ICR; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D013347:Subcellular Fractions / Q000648:ultrastructure*; D041623:Tomography, Optical Coherence / Q000295:instrumentation*