Evaluation of bioactive potential of an Aloe vera sterol extract.
We prepared a crude gel material from Aloe vera succulent leaf tissues. The ethanolic extract of lyophilized A. vera gel was used for the GC-MS analysis. Hexadecanoic acid (22.22%) was identified as major compound. Sitosterol and stigmasterol were found to be 2.89% and 2.1% in the extract. HPLC analysis was carried out to confirm the presence of stigmasterol. The concentration of sterol extract needed to scavenge DPPH free radical by 50% was calculated as 5.2 mg mL(-1). In the FRAP assay, the sterol extract showed significant hydroxyl radical scavenging in a dose-dependent manner (IC50 value 1.17 µg mL(-1)). Concentration of the sample required to reduce lipid peroxidation was found to be 4.18 µg mL(-1), and the extract also possessed acetylcholinesterase activity (IC50 - 5.26 µg mL(-1)). Catalase activity was 0.196 μM H2 O2 decomposed min(-1) µg(-1) protein, whereas the peroxidase activity was 17.01 μM of pyragallol oxidized min(-1) µg(-1) protein. The extract recorded higher activity against growth of S. greseus and C. albicans in the experiments carried out to determine antibacterial and antifungal activity, respectively.
School of Biosciences and Technology, VIT University, Vellore, 632014, India.
Raksha
R
Bawankar
V C
VC
Deepti
Pooja
P
Singh
Rathinasamy
R
Subashkumar
Govindasamy
G
Vivekanandhan
Subramanian
S
Babu
D000110:Acetylcholinesterase / Q000032:analysis / Q000378:metabolism; D000504:Aloe / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D000935:Antifungal Agents / Q000032:analysis / Q000494:pharmacology*; D002374:Catalase / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D016166:Free Radical Scavengers / Q000032:analysis / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D005782:Gels / Q000737:chemistry; D015227:Lipid Peroxidation; D019308:Palmitic Acid / Q000032:analysis; D009195:Peroxidase / Q000032:analysis / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D012855:Sitosterols / Q000032:analysis; D013265:Stigmasterol / Q000032:analysis
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D016166:Free Radical Scavengers; D005782:Gels; D010936:Plant Extracts; D012855:Sitosterols; D019308:Palmitic Acid; C025473:gamma-sitosterol; D013265:Stigmasterol; D002374:Catalase; D009195:Peroxidase; D000110:Acetylcholinesterase