Surface localisation of photosensitisers on intraocular lens biomaterials for prevention of infectious endophthalmitis and retinal protection.
Cataract surgery is one of the most commonly-practiced surgical procedures in Western medicine, and, while complications are rare, the most serious is infectious postoperative endophthalmitis. Bacteria may adhere to the implanted intraocular lens (IOL) and subsequent biofilm formation can lead to a chronic, difficult to treat infection. To date, no method to reduce the incidence of infectious endophthalmitis through bacterial elimination, while retaining optical transparency, has been reported. In this study we report a method to optimise the localisation of a cationic porphyrin at the surface of suitable acrylate copolymers, which is the first point of contact with potential pathogens. The porphyrin catalytically generates short-lived singlet oxygen, in the presence of visible light, which kills adherent bacteria indiscriminately. By restricting the photosensitiser to the surface of the biomaterial, reduction in optical transparency is minimised without affecting efficacy of singlet oxygen production. Hydrogel IOL biomaterials incorporating either methacrylic acid (MAA) or methyl methacrylate (MMA) co-monomers allow tuning of the hydrophobic and anionic properties to optimise the localisation of porphyrin. Physiochemical and antimicrobial properties of the materials have been characterised, giving candidate materials with self-generating, persistent anti-infective character against Gram-positive and Gram-negative organisms. Importantly, incorporation of porphyrin can also serve to protect the retina by filtering damaging shortwave visible light, due to the Soret absorption (λmax 430 nm).
School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK. c.mccoy@qub.ac.uk
Colin P
CP
McCoy
Rebecca A
RA
Craig
Seana M
SM
McGlinchey
Louise
L
Carson
David S
DS
Jones
Sean P
SP
Gorman
D000179:Acrylates / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001422:Bacterial Adhesion / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry; D009877:Endophthalmitis / Q000517:prevention & control*; D006801:Humans; D007910:Lenses, Intraocular / Q000382:microbiology*; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000494:pharmacology; D011166:Porphyrins / Q000008:administration & dosage* / Q000494:pharmacology; D044366:Transition Temperature; D014867:Water / Q000737:chemistry
D000179:Acrylates; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D017319:Photosensitizing Agents; D011166:Porphyrins; D014867:Water