Hyperbranched conjugated polyelectrolyte for dual-modality fluorescence and magnetic resonance cancer imaging.
Herein is reported the synthesis of gadolinium ion (Gd(III))-chelated hyperbranched conjugated polyelectrolyte (HCPE-Gd) and its application in fluorescence and magnetic resonance (MR) dual imaging in live animals. The synthesized HCPE-Gd forms nanospheres with an average diameter of ∼42 nm measured by laser light scattering and a quantum yield of 10% in aqueous solution. The absorption spectrum of HCPE-Gd has two maxima at 318 and 417 nm, and its photoluminescence maximum centers at 591 nm. Confocal laser scanning microscopy studies indicate that the HCPE-Gd is internalized in MCF-7 cancer cell cytoplasm with good photostability and low cytotoxicity. Further fluorescence and MR imaging studies on hepatoma H22 tumor-bearing mouse model reveal that HCPE-Gd can serve as an efficient optical/MR dual-modal imaging nanoprobe for in vivo cancer diagnosis.
Department of Chemical and Biomolecular Engineering, National University of Singapore, 117576, Singapore.
Dan
D
Ding
Guan
G
Wang
Jianzhao
J
Liu
Kai
K
Li
Kan-Yi
KY
Pu
Yong
Y
Hu
Jason C Y
JC
Ng
Ben Zhong
BZ
Tang
Bin
B
Liu
D000818:Animals; D001835:Body Weight; D045744:Cell Line, Tumor; D004573:Electrolytes; D005456:Fluorescent Dyes / Q000737:chemistry; D005682:Gadolinium / Q000737:chemistry; D006801:Humans; D049449:Luminescence; D061986:MCF-7 Cells; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000175:diagnosis* / Q000473:pathology*; D010777:Photochemistry / Q000379:methods; D011108:Polymers / Q000737:chemistry; D011789:Quantum Theory
D004573:Electrolytes; D005456:Fluorescent Dyes; D011108:Polymers; D005682:Gadolinium