State-dependent modulation of breathing in urethane-anesthetized rats.
Respiratory activity is most fragile during sleep, in particular during paradoxical [or rapid eye movement (REM)] sleep and sleep state transitions. Rats are commonly used to study respiratory neuromodulation, but rodent sleep is characterized by a highly fragmented sleep pattern, thus making it very challenging to examine different sleep states and potential pharmacological manipulations within them. Sleep-like brain-state alternations occur in rats under urethane anesthesia and may be an effective and efficient model for sleep itself. The present study assessed state-dependent changes in breathing and respiratory muscle modulation under urethane anesthesia to determine their similarity to those occurring during natural sleep. Rats were anesthetized with urethane and respiratory airflow, as well as electromyographic activity in respiratory muscles were recorded in combination with local field potentials in neocortex and hippocampus to determine how breathing pattern and muscle activity are modulated with brain state. Measurements were made in normoxic, hypoxic, and hypercapnic conditions. Results were compared with recordings made from rats during natural sleep. Brain-state alternations under urethane anesthesia were closely correlated with changes in breathing rate and variability and with modulation of respiratory muscle tone. These changes closely mimicked those observed in natural sleep. Of great interest was that, during both REM and REM-like states, genioglossus muscle activity was strongly depressed and abdominal muscle activity showed potent expiratory modulation. We demonstrate that, in urethane-anesthetized rats, respiratory airflow and muscle activity are closely correlated with brain-state transitions and parallel those shown in natural sleep, providing a useful model to systematically study sleep-related changes in respiratory control.
Department of Physiology, Centre for Neuroscience, and Women and Children's Health Research Institute, University of Alberta, Edmonton, Alberta T6G 2E1, Canada. silviap@ualberta.ca
Silvia
S
Pagliardini
John J
JJ
Greer
Gregory D
GD
Funk
Clayton T
CT
Dickson
D000009:Abdominal Muscles / Q000187:drug effects / Q000502:physiology; D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D058256:Brain Waves / Q000187:drug effects* / Q000502:physiology; D004569:Electroencephalography; D004576:Electromyography; D006935:Hypercapnia / Q000139:chemically induced / Q000503:physiopathology; D000860:Hypoxia / Q000503:physiopathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012119:Respiration / Q000187:drug effects*; D012894:Sleep Stages / Q000187:drug effects*; D013057:Spectrum Analysis; D013990:Tidal Volume / Q000187:drug effects; D014520:Urethane / Q000494:pharmacology*; D014851:Wakefulness / Q000187:drug effects / Q000502:physiology
D018686:Anesthetics, Intravenous; D014520:Urethane