Stable sugar-chain-immobilized fluorescent nanoparticles for probing lectin and cells.
Sugar chains are important molecules in cellular recognition and signaling, and quantum dots (QDs) are a very powerful tool for in vitro and in vivo imaging. Herein, we report the preparation of stable sugar-chain-immobilized fluorescent nanoparticles (SFNPs) and their application to the analysis of sugar-chain-protein interactions and cellular imaging. SFNPs were easily prepared by mixing CdTe/CdS core/shell QDs with our previously developed sugar-chain-ligand conjugates. The obtained SFNPs were very stable and could be stored for several months. In the binding analysis, β-galactose- and α-glucose-immobilized SFNPs were specifically interacted with Ricinus communis agglutinin I and concanavalin A, respectively, and made into aggregates. The binding interaction was detected visually, fluorescently, or both. In the experiment for cellular imaging, it was found that SFNPs were predominantly taken up by human hepatocyto carcinoma cells (HepG2), suggesting the possible usage of our designed SFNPs for various biochemical analyses of sugar chains.
Department of Chemistry, Biotechnology and Chemical Engineering, Kagoshima University, 1-21-40 Kohrimoto, Kagoshima 890-0065, Japan.
Hiroyuki
H
Shinchi
Masahiro
M
Wakao
Sho
S
Nakagawa
Eiko
E
Mochizuki
Susumu
S
Kuwabata
Yasuo
Y
Suda
D019187:Cadmium Compounds / Q000737:chemistry; D003208:Concanavalin A / Q000737:chemistry* / Q000378:metabolism; D005690:Galactose / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D018613:Microscopy, Confocal; D037121:Plant Lectins / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry
D019187:Cadmium Compounds; D037121:Plant Lectins; C017707:Ricinus communis agglutinin-1; D018036:Selenium Compounds; D003208:Concanavalin A; C058667:cadmium selenide; D005947:Glucose; D013691:Tellurium; C028337:cadmium telluride; D005690:Galactose