Comparative genome analysis between Aspergillus oryzae strains reveals close relationship between sites of mutation localization and regions of highly divergent genes among Aspergillus species.
Aspergillus oryzae has been utilized for over 1000 years in Japan for the production of various traditional foods, and a large number of A. oryzae strains have been isolated and/or selected for the effective fermentation of food ingredients. Characteristics of genetic alterations among the strains used are of particular interest in studies of A. oryzae. Here, we have sequenced the whole genome of an industrial fungal isolate, A. oryzae RIB326, by using a next-generation sequencing system and compared the data with those of A. oryzae RIB40, a wild-type strain sequenced in 2005. The aim of this study was to evaluate the mutation pressure on the non-syntenic blocks (NSBs) of the genome, which were previously identified through comparative genomic analysis of A. oryzae, Aspergillus fumigatus, and Aspergillus nidulans. We found that genes within the NSBs of RIB326 accumulate mutations more frequently than those within the SBs, regardless of their distance from the telomeres or of their expression level. Our findings suggest that the high mutation frequency of NSBs might contribute to maintaining the diversity of the A. oryzae genome.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi-Nijo 17-2-1, Tsukisamu, Sapporo, Hokkaido 062-8517, Japan.
Myco
M
Umemura
Hideaki
H
Koike
Noriko
N
Yamane
Yoshinori
Y
Koyama
Yuki
Y
Satou
Ikuya
I
Kikuzato
Morimi
M
Teruya
Masatoshi
M
Tsukahara
Yumi
Y
Imada
Youji
Y
Wachi
Yukino
Y
Miwa
Shuichi
S
Yano
Koichi
K
Tamano
Yutaka
Y
Kawarabayasi
Kazuhiro E
KE
Fujimori
Masayuki
M
Machida
Takashi
T
Hirano
D001236:Aspergillus oryzae / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D016681:Genome, Fungal / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D009154:Mutation / Q000235:genetics; D059645:Mutation Rate; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D026801:Synteny / Q000235:genetics; D016615:Telomere / Q000235:genetics