Terrimonas pekingensis sp. nov., isolated from bulking sludge, and emended descriptions of the genus Terrimonas, Terrimonas ferruginea, Terrimonas lutea and Terrimonas aquatica.
A Gram-negative, strictly aerobic, non-motile and non-spore-forming rod that produced white, viscous colonies, designated QH(T), was isolated from bulking sludge collected from a municipal wastewater treatment plant in Beijing, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain QH(T) belonged to the genus Terrimonas and shared 96.3 % sequence similarity with Terrimonas lutea DY(T), 94.1 % with Terrimonas ferruginea ATCC 13524(T) and 93.8 % with Terrimonas aquatica RIB1-6(T). Strain QH(T) contained iso-C15 : 0, summed feature 3 (comprising one or more of C16 : 1ω7c, C16 : 1ω6c and iso-C15 : 0 2-OH) and iso-C15 : 1 G as the predominant fatty acids. The predominant polar lipid of strain QH(T) and members of the genus Terrimonas was phosphatidylethanolamine. The major isoprenoid quinone of strain QH(T) was MK-7 and the DNA G+C content was 41.0 mol%. DNA-DNA relatedness between strain QH(T) and T. lutea BCRC 17944(T), T. ferruginea BCRC 17943(T) and T. aquatica BCRC 17941(T) was 32, 23 and 22 %, respectively. On the basis of phylogenetic inference, differential phenotypic data and low DNA-DNA relatedness with members of the genus Terrimonas, strain QH(T) represents a novel species, for which the name Terrimonas pekingensis sp. nov. is proposed. The type strain is QH(T) ( = CICC 10452(T)  = NCCB 100397(T)). The descriptions of the genus Terrimonas and T. ferruginea, T. lutea and T. aquatica are also emended.
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.|College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, PR China.
Decai
D
Jin
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, PR China.
Ping
P
Wang
School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.|Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
Zhihui
Z
Bai
School of Chemical Engineering, The University of Adelaide, Adelaide, SA 5005, Australia.
Bo
B
Jin
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, PR China.
Zhisheng
Z
Yu
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
Xinxin
X
Wang
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.
Guoqiang
G
Zhuang
Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China.|College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, PR China.
Hongxun
H
Zhang
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7