Microsatellite  markers for assessing genetic diversity of the medicinal plant Paris  polyphylla var. chinensis (Trilliaceae).
Paris  polyphylla var. chinensis is a perennial herb with  medicinal properties that is widely used in traditional Chinese medicine.  However, this plant has been on the edge of extinction during the last few  decades because of excessive deforestation based on the intense ethnopharmaceutical  interest. We isolated 12 microsatellite loci from a (CT)(n)-enriched  genomic library of P. polyphylla var. chinensis. The polymorphism  at each locus was analyzed by screening 30 individuals from a natural  population. The number of alleles ranged from 2 to 5. The observed and expected  heterozygosities ranged from 0.000 to 0.467, with a mean of 0.247, and from  0.383 to 0.662 with a mean of 0.537, respectively. Six loci (Pp1, Pp3, Pp6, Pp7, Pp9, and Pp12) were found to  significantly deviate from Hardy-Weinberg equilibrium. This may be due to the  small population size, inbreeding or null alleles. Five of the pairwise  comparisons (Pp1 and Pp4, Pp2 and Pp5, Pp2 and Pp9, Pp2 and Pp12, Pp11 and Pp12) exhibited  significant linkage disequilibrium (P < 0.05). We conclude that these microsatellite  markers will be useful for population genetic studies of P. polyphylla var. chinensis.
Key Laboratory of Molecular Evolution and Biodiversity, Institute of Molecular Biology and Biotechnology, Anhui Normal University, Wuhu, P.R. China.
J Y
JY
Zheng
H
H
Wang
X X
XX
Chen
P
P
Wang
P
P
Gao
X N
XN
Li
G P
GP
Zhu
D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D019668:Liliaceae / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal / Q000235:genetics*
D005819:Genetic Markers