Lentivirus-mediated short-hairpin RNA targeting IGF-1R inhibits growth             and lymphangiogenesis in breast cancer.
In this study, we investigated the effects of lentivirus (LV)-mediated short             hairpin RNA (shRNA) targeting IGF-1R on the growth and lymphangiogenesis of breast             cancer. The LV vector effectively delivered the IGF-1R shRNA to MDA-MB‑231 cells,             leading to significant reduction of IGF-1R mRNA and protein expression. Infection             of MDA-MB-231 cells with LV-IGF-1R shRNA reduced cell growth and migration. Transplantation             of MDA-MB-231 cells with suppressed IGF-1R expression in SCID mice reduced tumor             growth and lymphangiogenesis. These data collectively suggest that LV-mediated             shRNA is an effective way to suppress IGF-1R expression and to inhibit growth             and lymphangiogenesis of breast cancer. Specific inhibition of IGF-1R expression             with shRNA represents a promising approach for the treatment of breast cancer.
Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, HuangPu District, Shanghai 200011, PR China.
Yaning
Y
Chen
Chenfang
C
Zhu
Zhiyou
Z
Peng
Yalei
Y
Dai
Yan
Y
Gu
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D005822:Genetic Vectors; D057809:HEK293 Cells; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D042583:Lymphangiogenesis; D015674:Mammary Neoplasms, Animal / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D051379:Mice; D016513:Mice, SCID; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering; D017526:Receptor, IGF Type 1 / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D034741:RNA, Small Interfering; D017526:Receptor, IGF Type 1