Peritubular cells may modulate Leydig cell-mediated testosterone production through a nonclassic pathway.
OBJECTIVE
To evaluate whether paracrine signals are responsible for hormone-independent Leydig cell (Lc) steroidogenesis in the testis.


METHODS
Testicular peritubular cells (PTc), Sertoli cells (Sc), and Lc were isolated and cultured, and their effect on each other was evaluated in terms of lactate production by Sc and testosterone (T) production by Lc.


METHODS
Research institution.


METHODS
Wistar rats.


METHODS
Testes were surgically removed, and a new, easily adoptable procedure for PTc was developed; culture media from Sc, PTc, and Lc cultures were used for treating pure populations of these cells. Cells were also cocultured together.


METHODS
To assess culture or coculture supernatants for presence of metabolites and Lc messenger RNA analysis.


RESULTS
Although PTc secreted factor(s) did not augment production of Sc lactate, essential for germ cell survival, they significantly augmented T secretion by Lc, independent of StAR gene expression. Coculture studies showed that T production by Lc was significantly stimulated when Lc were cocultured with PTc, even in the absence of hormones.


CONCLUSIONS
Testicular peritubular cell-derived factor(s) can potentially augment T production by Lc in a nonclassic manner even in a gonadotropin-deficient environment.
Division of Cellular Endocrinology, National Institute of Immunology, New Delhi, India.
Jibanananda
J
Mishra
Mukesh
M
Gautam
Rajesh
R
Dadhich
Bhavani S
BS
Kowtharapu
Subeer S
SS
Majumdar
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000378:metabolism; D005786:Gene Expression Regulation; D019344:Lactic Acid / Q000378:metabolism; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D019899:Paracrine Communication; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012708:Sertoli Cells / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*; D000098682:Steroidogenic Acute Regulatory Protein
D017077:Culture Media, Conditioned; D010750:Phosphoproteins; D012333:RNA, Messenger; D000098682:Steroidogenic Acute Regulatory Protein; D019344:Lactic Acid; D013739:Testosterone