Cardiovascular effect is independent of hemolytic toxicity of tentacle-only extract from the jellyfish Cyanea capillata.
Our previous studies have confirmed that the crude tentacle-only extract (cTOE) from the jellyfish Cyanea capillata (Cyaneidae) exhibits hemolytic and cardiovascular toxicities simultaneously. So, it is quite difficult to discern the underlying active component responsible for heart injury caused by cTOE. The inactivation of the hemolytic toxicity from cTOE accompanied with a removal of plenty of precipitates would facilitate the separation of cardiovascular component and the investigation of its cardiovascular injury mechanism. In our research, after the treatment of one-step alkaline denaturation followed by twice dialysis, the protein concentration of the treated tentacle-only extract (tTOE) was about 1/3 of cTOE, and SDS-PAGE showed smaller numbers and lower density of protein bands in tTOE. The hemolytic toxicity of tTOE was completely lost while its cardiovascular toxicity was well retained. The observations of cardiac function, histopathology and ultrastructural pathology all support tTOE with significant cardiovascular toxicity. Blood gas indexes and electrolytes changed far less by tTOE than those by cTOE, though still with significant difference from normal. In summary, the cardiovascular toxicity of cTOE can exist independently of the hemolytic toxicity and tTOE can be employed as a better venom sample for further purification and mechanism research on the jellyfish cardiovascular toxic proteins.
Department of Chemical Defense Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China.
Xiao
X
Liang
Wang
W
Beilei
Li
L
Ying
Wang
W
Qianqian
Liu
L
Sihua
Wang
W
Yang
Liu
L
Guoyan
Lu
L
Jia
Ye
Y
Xuting
Zhang
Z
Liming
D000818:Animals; D001784:Blood Gas Analysis; D002319:Cardiovascular System / Q000187:drug effects*; D003064:Cnidarian Venoms / Q000633:toxicity*; D006352:Heart Ventricles / Q000187:drug effects; D006461:Hemolysis; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D037401:Scyphozoa / Q000378:metabolism*
D003064:Cnidarian Venoms