[Effect of Ras signal regulating Cox7a2 expression and its sub-cellular location in testicular interstitial cell].
OBJECTIVE
To investigate the co-sub-cellular-location of Cox7a2 and Ras.


METHODS
Ras and its mutant plasmid were cloned by RT-PCR and sequence analysis. Cox7a2-pEYFP-N1, Ras-pEYFP-N1 and N17-Ras-pEYFP-N1 fluorescent protein vectors were constructed and transfected into TM3 cells.


RESULTS
Cox7a2 was located in the mitochondria, but its location was changed by the expression of Ras. When the dominant negative ras was expressed in the cells, the Cox7a2 located into the mitochondria again.


CONCLUSIONS
Cox7a2 mediated testosterone production, which might be at least in part related with the Ras signaling pathway. Ras may be the regulating target and further investigation is needed to make it clear.
Reproduction and Genetic Medical Center, Peking University First Hospital, Beijing, China. bdyychenliang@163.com
Liang
L
Chen
Yang
Y
Xu
Wen-li
WL
Zuo
Hui-xia
HX
Yang
Qin-ping
QP
Liao
Zhong-cheng
ZC
Xin
Ying-lu
YL
Guo
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D007006:Hypogonadism / Q000235:genetics / Q000378:metabolism; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D015398:Signal Transduction; D013739:Testosterone / Q000096:biosynthesis; D014162:Transfection; D018631:ras Proteins / Q000235:genetics* / Q000502:physiology*
D013739:Testosterone; C514296:Cox7a2 protein, mouse; D003576:Electron Transport Complex IV; D018631:ras Proteins