Methods applied to the study of protein arginine methylation.
Arginine methylation was discovered in the mid-1960s. About 15 years ago, the first protein arginine N-methyltransferase (PRMT) enzyme was described. The PRMT family now stands at nine members, and these enzymes play a key role in regulating a multitude of cellular events. The majority of the PRMTs have been deleted in mice, thus providing genetically tractable systems for in vivo and cell-based studies. These studies have implicated this posttranslational modification in chromatin remodeling, transcriptional regulation, RNA processing, protein/RNA trafficking, signal transduction, and DNA repair. In this chapter, we introduce different approaches that have been developed to assess protein arginine methylation levels and characterize PRMT substrates.
Department of Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, Texas, USA. dcheng@mdanderson.org
Donghang
D
Cheng
Vidyasiri
V
Vemulapalli
Mark T
MT
Bedford
D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry; D001120:Arginine / Q000737:chemistry*; D002457:Cell Extracts / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000737:chemistry; D057075:Enzyme Assays; D004791:Enzyme Inhibitors / Q000737:chemistry; D004926:Escherichia coli; D005347:Fibroblasts / Q000201:enzymology; D055786:Gene Knockout Techniques; D006367:HeLa Cells; D006801:Humans; D008745:Methylation; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D011484:Protein-Arginine N-Methyltransferases / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry*; D012156:Reticulocytes / Q000201:enzymology
D058846:Antibodies, Monoclonal, Murine-Derived; D002457:Cell Extracts; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D001120:Arginine; D011484:Protein-Arginine N-Methyltransferases; C119966:coactivator-associated arginine methyltransferase 1