Quantitative proteomic analysis of pregnancy-related proteins from peripheral blood mononuclear cells during pregnancy in pigs.
Information obtained from peripheral blood could help us understand the underlying mechanisms in autoimmune diseases, cancer, pregnancy, and other conditions. In this paper, we present the protein map of porcine peripheral blood mononuclear cells (PBMC) to better understand the molecular expression changes that occur during pregnancy using proteomic analysis. We detected 94 differentially expressed proteins in pregnant vs. non-pregnant (NP) pigs, and a representative set of the proteins was subjected to LC-MS/MS analysis. Furthermore, the identified proteins were categorized according to their biological process and molecular function. By classifying the proteins according to their functions, a large number of differentially regulated proteins involved in anti-oxidant, detoxification and stress response pathways were found, including peroxiredoxin (PRX) 1, 2, and 6, glutathione-S-transferase (GST), annexin A2, and A6, and heat shock protein 27 (HSP 27) during pregnancy (pregnancy d of E40, embryonic day 40; E70, embryonic day 70; and E93, embryonic day 93) compared with non-pregnancy. In this study, a proteomic approach utilizing 2-DE and LC-MS/MS was applied to evaluate specific molecular expression changes during pregnancy compared with non-pregnancy. Together, these data offer new information about the proteome map and factors that are differentially regulated during maintenance of normal pregnancy.
Department of Oral Pharmacology, School of Dentistry and Institute of Dental Bioscience, BK21 Project, Chonbuk National University, Jeonju, Republic of Korea.
Jung-Il
JI
Chae
Jumi
J
Kim
Seong Goo
SG
Lee
Min Whan
MW
Koh
Young-Joo
YJ
Jeon
Dong-Wook
DW
Kim
Seon Mi
SM
Ko
Kang Seok
KS
Seo
Hak Kyo
HK
Lee
Nag-Jin
NJ
Choi
Seong-Keun
SK
Cho
Joohyun
J
Ryu
Sunghyun
S
Kang
Dong-Seok
DS
Lee
Hyung-Min
HM
Chung
Deog-Bon
DB
Koo
D000818:Animals; D001798:Blood Proteins / Q000032:analysis* / Q000145:classification / Q000378:metabolism; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D007963:Leukocytes, Mononuclear / Q000737:chemistry* / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis / Q000097:blood* / Q000145:classification / Q000378:metabolism; D011270:Pregnancy, Animal / Q000097:blood / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D013552:Swine / Q000097:blood / Q000378:metabolism / Q000502:physiology; D053719:Tandem Mass Spectrometry
D001798:Blood Proteins; D011257:Pregnancy Proteins; D020543:Proteome