Fusarium verticillioides from finger millet in Uganda.
Finger millet (Eleusine coracana) is a subsistence crop grown in Sub-Saharan Africa and the Indian Sub-continent. Fusarium species occurring on this crop have not been reported. Approximately 13% of the Fusarium isolates recovered from finger millet growing at three different locations in eastern Uganda belong to Fusarium verticillioides, and could produce up to 18,600 µg/g of total fumonisins when cultured under laboratory conditions. These strains are all genetically unique, based on AFLP analyses, and form fertile perithecia when crossed with the standard mating type tester strains for this species. All but one of the strains is female-fertile and mating-type segregates 13:20 Mat-1:Mat-2. Three new sequences of the gene encoding translation elongation factor 1-α were found within the population. These results indicate a potential health risk for infants who consume finger millet gruel as a weaning food, and are consistent with the hypothesis that F. verticillioides originated in Africa and not in the Americas, despite its widespread association with maize grown almost anywhere worldwide.
Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506-5502, USA.
Amgad A
AA
Saleh
J P
JP
Esele
Antonio
A
Logrieco
Alberto
A
Ritieni
John F
JF
Leslie
D000821:Animal Feed / Q000032:analysis / Q000382:microbiology; D000818:Animals; D018556:Crops, Agricultural / Q000254:growth & development / Q000382:microbiology*; D031748:Eleusine / Q000254:growth & development / Q000382:microbiology*; D005506:Food Contamination; D005516:Food Microbiology; D037341:Fumonisins / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D013045:Species Specificity; D014454:Uganda
D037341:Fumonisins; D005656:Fungal Proteins; D007527:Isoenzymes; D020648:Peptide Elongation Factor 1; C056934:fumonisin B2; C056933:fumonisin B1; D009713:Nucleotidyltransferases; C111407:mat1 maturase