Transcriptome and coexpression network analysis of the human glioma cell line Hs683 exposed to candoxin.
OBJECTIVE
Gliomas are the most common primary tumours of the central nervous system. Snake venom, such as candoxin (CDX) isolated from Bungarus candidus, inhibits glioma cell proliferation. This study explored the gene regulation profile of CDX-treated human glioma Hs683 cells.


METHODS
Using microarray technology and bioinformatics analyses the underlying molecular mechanism of action of CDX was evaluated by constructing gene regulation and protein-protein interaction co expression networks.


RESULTS
CDX treatment induced a large number of related genes at  the transcriptional level. The MYC gene (v-myc myelocytomatosis viral oncogene homologue [avian]) had a key role in the response of Hs683 cells to CDX treatment. Activation of MYC upregulated NDRG1 (N-myc downstream regulated 1), WNT10B (wingless-type mouse mammary  tumour virus integration site family, member 10B), CASP9 (caspase 9, apoptosis-related cysteine peptidase) and CDKN2A (cyclin-dependent kinase inhibitor 2A), and downregulated ID3 (inhibitor of DNA binding 3, dominant negative helix-loop-helix protein) and  SLC1A4 (solute carrier family 1 [glutamate/neutral amino acid transporter], member 4). In addition, a subnetwork was constructed among SPP1 (secreted phosphoprotein 1), SDC1 (syndecan 1) and CD44 based on protein-protein interactions, and these genes were  predicted to be involved in glioma cell invasion.


CONCLUSIONS
These findings might provide novel therapeutic targets for glioma chemotherapy.
Department of Neurosurgery, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Y X
YX
Jiang
Y
Y
Ma
Y
Y
Cheng
D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012910:Snake Venoms / Q000494:pharmacology*; D059467:Transcriptome
D012910:Snake Venoms; C459604:candoxin