Association between β2-adrenoceptor (ADRB2) haplotypes and insulin resistance in PCOS.
OBJECTIVE
The aim of this study was to explore β2-adrenoceptor (ADRB2) haplotype associations with phenotypes and quantitative traits related to insulin resistance (IR) and the metabolic syndrome (MS) in a polycystic ovary syndrome (PCOS) population. A secondary purpose was to assess the association between ADRB2 haplotype and PCOS.


METHODS
Genetic polymorphism analysis. Cross-sectional case-control association study.


METHODS
Medical University Hospital and research laboratory.


METHODS
One hundred and sixty-five unrelated women with PCOS and 116 unrelated women without PCOS (control sample).


METHODS
Clinical and biochemical measurements, and ADRB2 genotyping in PCOS patients and control subjects.


METHODS
ADRB2 haplotypes (comprising rs1042711, rs1801704, rs1042713 and rs1042714 in that order), genotyping and statistical analysis to evaluate associations with continuous variables and traits related to IR and MS in a PCOS population. Associations between ADRB2 haplotypes and PCOS were also assessed.


RESULTS
We observed an age-adjusted association between ADRB2 haplotype CCGG and lower insulin (P = 0·018) and HOMA (P = 0·008) in the PCOS sample. Interestingly, the expected differences in surrogate measures of IR between cases and controls were not significant in CCGG/CCGG carriers. In the case-control study, genotype CCGG/CCGG was associated with a 14% decrease in PCOS risk (P = 0·043), taking into account confounding variables.


CONCLUSIONS
Haplotype I (CCGG) has a protective role for IR and MS in PCOS.
Genetics and Molecular Biology, Department of Microbiology, Immunology and Biotechnology, School of Pharmacy and Biochemistry, University of Buenos Aires (UBA), Buenos Aires, Argentina.
Mariana L
ML
Tellechea
Damián O
DO
Muzzio
Andrea E
AE
Iglesias Molli
Susana H
SH
Belli
Mabel N
MN
Graffigna
Oscar A
OA
Levalle
Gustavo D
GD
Frechtel
Gloria E
GE
Cerrone
D000328:Adult; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006239:Haplotypes; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D024821:Metabolic Syndrome / Q000453:epidemiology / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D011085:Polycystic Ovary Syndrome / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D015995:Prevalence; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics* / Q000378:metabolism
C553443:ADRB2 protein, human; D018343:Receptors, Adrenergic, beta-2