Optimisation of batch culture conditions for cell-envelope-associated proteinase production from Lactobacillus delbrueckii subsp. lactis ATCC® 7830™.
Using a combination of conventional sequential techniques, the batch growth conditions for the production of cell-envelope-associated proteinases have for the first time been studied and optimised in Lactobacillus delbrueckii subsp. lactis 313 (ATCC 7830; LDL 313). Concentrations of inoculum (0.1 < X < 10 % vol/vol), agitation speed (0 < S < 200 rpm), varying incubation temperature (30 < T < 50 °C), starting pH (4.5 < pH < 7) and carbon/nitrogen ratio of production medium (0.2 < r < 5) had an individual effect on proteinase yield (p < 0.01). Optimal conditions for proteinase production included an initial pH of 6.0, 45 °C incubation temperature, 2 % (v/v) inoculum size of OD(560) = 1, 150 rpm agitation speed, and growth medium carbon/nitrogen ratio of 1.0. Maximum proteinase activity obtained for whole cells was 0.99 U/ml after 8 h of incubation. The variables studied are very relevant due to their significance in improving the productivity of proteinase synthesis from LDL 313, under process and, likely, economic optimum conditions.
Bioengineering Laboratory, Department of Chemical Engineering, Monash University, Wellington Road, Clayton, VIC 3800, Australia. dominic.agyei@monash.edu
Dominic
D
Agyei
Ravichandra
R
Potumarthi
Michael K
MK
Danquah
D061249:Batch Cell Culture Techniques; D002473:Cell Wall / Q000201:enzymology*; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D048668:Lactobacillus delbrueckii / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000096:biosynthesis* / Q000737:chemistry; D013696:Temperature
D003470:Culture Media; D010447:Peptide Hydrolases