Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of colonies using the met30 strain.
Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium with a high methionine content.
Russian State Collection of Industrial Microorganisms (VKPM), State Research Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhny pr., 1, 117545 Moscow, Russia.
Marina G
MG
Tarutina
Tatiana A
TA
Dutova
Inna E
IE
Yezhova
Hiroaki
H
Nishiuchi
Sergey P
SP
Sineoky
D000483:Alleles; D002469:Cell Separation / Q000379:methods; D003116:Color; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D044783:F-Box Proteins / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics*; D005978:Glutathione / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008715:Methionine / Q000378:metabolism / Q000494:pharmacology; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013455:Sulfur / Q000032:analysis*; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics*
D003470:Culture Media; D044783:F-Box Proteins; C470285:MET30 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D013455:Sulfur; D008715:Methionine; D043743:Ubiquitin-Protein Ligase Complexes; D005978:Glutathione