Solid-phase colorimetric method for the quantification of fucoidan.
We described the simple, selective, and rapid method for determination of fucoidans using methylene blue staining of sulfated polysaccharides, immobilized into filter paper and consequent optic density (at A                 (663) nm) measurement of the eluted dye from filter paper. This solid-phase method allows selective determination of 1-20 μg fucoidan in presence of potentially interfering compounds (alginic acid, DNA, salts, proteins, and detergents). Further, we demonstrated the alternative way of using image processing software for fucoidan quantification without extraction of methylene blue dye from stained spots of fucoidan-dye complex.
Graduate School of Biotechnology, Kyung Hee University, Yongin, Republic of Korea.
Jung Min
JM
Lee
Z-U
ZU
Shin
Gafurjon T
GT
Mavlonov
Ibrokhim Y
IY
Abdurakhmonov
Tae-Hoo
TH
Yi
D000464:Alginates / Q000737:chemistry; D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry; D003902:Detergents / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D008751:Methylene Blue / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification; D011506:Proteins / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D013194:Staining and Labeling; D013431:Sulfates / Q000737:chemistry / Q000302:isolation & purification
D000464:Alginates; D003902:Detergents; D006603:Hexuronic Acids; D011134:Polysaccharides; D011506:Proteins; D012492:Salts; D013431:Sulfates; D020723:Glucuronic Acid; D004247:DNA; C007789:fucoidan; D008751:Methylene Blue