Proteomic characterization of phagosomal membrane microdomains during phagolysosome biogenesis and evolution.
After their formation at the cell surface, phagosomes become fully functional through a complex maturation process involving sequential interactions with various intracellular organelles. In the last decade, series of data indicated that some of the phagosome functional properties occur in specialized membrane microdomains. The molecules associated with membrane microdomains, as well as the organization of these structures during phagolysosome biogenesis are largely unknown. In this study, we combined proteomics and bioinformatics analyses to characterize the dynamic association of proteins to maturing phagosomes. Our data indicate that groups of proteins shuffle from detergent-soluble to detergent-resistant membrane microdomains during maturation, supporting a model in which the modulation of the phagosome functional properties involves an important reorganization of the phagosome proteome by the coordinated spatial segregation of proteins.
Département de pathologie et biologie cellulaire, Université de Montréal, and Département de pédiatrie, Centre de recherche de l'hôpital Sainte-Justine, C.P. 6128, Succ centre ville, Montréal, Québec, H3C 3J7, Canada.
Guillaume
G
Goyette
Jonathan
J
Boulais
Nicholas J
NJ
Carruthers
Christian R
CR
Landry
Isabelle
I
Jutras
Sophie
S
Duclos
Jean-François
JF
Dermine
Stephen W
SW
Michnick
Sylvie
S
LaBoissière
Gilles
G
Lajoie
Luis
L
Barreiro
Pierre
P
Thibault
Michel
M
Desjardins
D000818:Animals; D002460:Cell Line; D003902:Detergents / Q000494:pharmacology; D019143:Evolution, Molecular; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism*; D021962:Membrane Microdomains / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D010455:Peptides / Q000378:metabolism; D010588:Phagosomes / Q000187:drug effects / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
D003902:Detergents; D010455:Peptides; D020543:Proteome