Chemical functionalization of bioceramics to enhance endothelial cells adhesion for tissue engineering.
To control the selective adhesion of human endothelial cells and human serum proteins to bioceramics of different compositions, a multifunctional ligand containing a cyclic arginine-glycine-aspartate (RGD) peptide, a tetraethylene glycol spacer, and a gallate moiety was designed, synthesized, and characterized. The binding of this ligand to alumina-based, hydroxyapatite-based, and calcium phosphate-based bioceramics was demonstrated. The conjugation of this ligand to the bioceramics induced a decrease in the nonselective and integrin-selective binding of human serum proteins, whereas the binding and adhesion of human endothelial cells was enhanced, dependent on the particular bioceramics.
Institute of Chemical Sciences and Engineering, EPFL, CH-1015, Lausanne, Switzerland.
Françoise
F
Borcard
Davide
D
Staedler
Horacio
H
Comas
Franziska Krauss
FK
Juillerat
Philip N
PN
Sturzenegger
Roman
R
Heuberger
Urs T
UT
Gonzenbach
Lucienne
L
Juillerat-Jeanneret
Sandrine
S
Gerber-Lemaire
D000537:Aluminum Oxide / Q000737:chemistry; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D002516:Ceramics / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry; D019855:Ethylene Glycol / Q000737:chemistry; D005707:Gallic Acid / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D008024:Ligands; D009842:Oligopeptides / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D002130:Calcium Phosphates; D008024:Ligands; D009842:Oligopeptides; D005707:Gallic Acid; C047981:arginyl-glycyl-aspartic acid; D017886:Durapatite; D019855:Ethylene Glycol; C018392:tricalcium phosphate; D000537:Aluminum Oxide