Deep ultraviolet resonant Raman imaging of a cell.
We report the first demonstration of deep ultraviolet (DUV) Raman imaging of a cell. Nucleotide distributions in a HeLa cell were observed without any labeling at 257 nm excitation with resonant bands attributable to guanine and adenine. Obtained images represent DNA localization at nucleoli in the nucleus and RNA distribution in the cytoplasm. The presented technique extends the potential of Raman microscopy as a tool to selectively probe nucleic acids in a cell with high sensitivity due to resonance.
RIKEN, Nanophotonics Laboratory, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Yasuaki
Y
Kumamoto
Atsushi
A
Taguchi
Nicholas Isaac
NI
Smith
Satoshi
S
Kawata
D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D009711:Nucleotides / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013347:Subcellular Fractions / Q000378:metabolism*
D009711:Nucleotides