[Effect of hydroxylated fullerene C60(OH)25 on macrophage plasma membrane integrity].
Our study has shown that the damaging effect of hydroxylated fullerene C60(OH)25 on mouse peritoneal macrophage plasma membranes increased when we enlarged the concentration of fullerene in the incubation media (from 0.005 to 0.5 mg/ml), the incubation temperature (from 22 degrees C to 37 degrees C) and the time of incubation (from 30 to 90 min). In conditions of the H2O2-induced membrane damage, fullerene was observed to intensify the H2O2-induced damaging effect at a concentration of 0.05 mg/ml and reduce it at a concentration of 0.5 mg/ml. In conditions of the UV-induced membrane damage, it was discovered that the damaging effect of UV increased when C60(OH)25 nanoparticles were added to the incubation media before irradiation and decreased when they were added after irradiation. Eventual participation of ROS in damaging effects of C60(OH)25 was discussed.
S K
SK
Pirutin
V B
VB
Turovetskiĭ
A V
AV
Kedrov
Iu B
IuB
Kudriashov
K V
KV
Shaĭtan
A B
AB
Rubin
D000818:Animals; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D002478:Cells, Cultured; D037741:Fullerenes / Q000633:toxicity*; D006358:Hot Temperature; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D008297:Male; D051379:Mice; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects; D013997:Time Factors; D014466:Ultraviolet Rays / Q000009:adverse effects
D037741:Fullerenes; D016877:Oxidants