A peptide receptor-based bioelectronic nose for the real-time determination of seafood quality.
We herein report a peptide receptor-based bioelectronic nose (PRBN) that can determine the quality of seafood in real-time through measuring the amount of trimethylamine (TMA) generated from spoiled seafood. The PRBN was developed using single walled-carbon nanotube field-effect transistors (SWNT-FETs) functionalized with olfactory receptor-derived peptides (ORPs) which can recognize TMA and it allowed us to sensitively and selectively detect TMA in real-time at concentrations as low as 10fM. Utilizing these properties, we were able to not only determine the quality of three kinds of seafood (oyster, shrimp, and lobster), but were also able to distinguish spoiled seafood from other types of spoiled foods without any pretreatment processes. Especially, the use of small synthetic peptide rather than the whole protein allowed PRBNs to be simply manufactured through a single-step process and to be reused with high reproducibility due to no requirement of lipid bilayers. Furthermore, the PRBN was produced on a portable scale making it effectively useful for the food industry where the on-site measurement of seafood quality is required.
School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Korea.
Jong Hyun
JH
Lim
Juhun
J
Park
Jung Ho
JH
Ahn
Hye Jun
HJ
Jin
Seunghun
S
Hong
Tai Hyun
TH
Park
D000595:Amino Acid Sequence; D004867:Equipment Design; D063427:Food Quality; D008744:Methylamines / Q000032:analysis*; D008969:Molecular Sequence Data; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D018035:Receptors, Odorant / Q000737:chemistry*; D017747:Seafood / Q000032:analysis*; D012680:Sensitivity and Specificity; D014173:Transistors, Electronic
D008744:Methylamines; D037742:Nanotubes, Carbon; D018035:Receptors, Odorant; C023336:trimethylamine