Engineering of therapeutic polypeptides through chemical synthesis: early lessons from human parathyroid hormone and analogues.
Application of chemical synthesis to gain access to high purity hPTH as well as more stable analogues was accomplished through a menu of extended NCL followed by metal free dethiylation.
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA.
Suwei
S
Dong
Shiying
S
Shang
Jianfeng
J
Li
Zhongping
Z
Tan
Thomas
T
Dean
Akira
A
Maeda
Thomas J
TJ
Gardella
Samuel J
SJ
Danishefsky
D000818:Animals; D002118:Calcium / Q000097:blood / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D010281:Parathyroid Hormone / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000494:pharmacology; D010455:Peptides / Q000008:administration & dosage / Q000138:chemical synthesis* / Q000494:pharmacology; D015202:Protein Engineering
D010281:Parathyroid Hormone; D010455:Peptides; D002118:Calcium