miR-182 inhibits Schwann cell proliferation and migration by targeting FGF9 and NTM, respectively at an early stage following sciatic nerve injury.
The regulation of Schwann cell (SC) responses to injury stimuli by microRNAs (miRNAs) remains to be explored. Here, we identified 17 miRNAs that showed dynamic expression alterations at five early time points following rat sciatic nerve resection. Then we analyzed the expression pattern of 17 miRNAs, and integrated their putative targets with differentially expressed mRNAs. The resulting 222 potential targets were mainly involved in cell phenotype modulation, including immune response, cell death and cell locomotion. Among 17 miRNAs, miR-182 expression was up-regulated. The enhanced expression of miR-182 was correlated with nerve injury-induced phenotype modulation of SCs. Further investigation revealed that fibroblast growth factor 9 (FGF9) and neurotrimin (NTM) were two direct targets of miR-182 in SCs, with miR-182 binding to the 3'-untranslated region of FGF9 and NTM. Silencing of FGF9 and NTM recapitulated the inhibiting effect of miR-182 mimics on SC proliferation and migration, respectively, whereas enforced knockdown of FGF9 and NTM reversed the promoting effect of miR-182 inhibitor on SC proliferation and migration, respectively. Our data indicate that nerve injury inhibits SC proliferation and migration through rapid regulation of miR-182 by targeting FGF9 and NTM, providing novel insights into the roles of miRNAs in nerve injury and repair.
Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China.
Bin
B
Yu
Tianmei
T
Qian
Yongjun
Y
Wang
Songlin
S
Zhou
Guohui
G
Ding
Fei
F
Ding
Xiaosong
X
Gu
D020413:3' Untranslated Regions; D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D051525:Fibroblast Growth Factor 9 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D058851:GPI-Linked Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D019006:Neural Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D012584:Sciatic Nerve / Q000293:injuries*
D020413:3' Untranslated Regions; C496461:Fgf9 protein, rat; D051525:Fibroblast Growth Factor 9; D058851:GPI-Linked Proteins; C577631:MIRN182 microRNA, rat; D035683:MicroRNAs; D019006:Neural Cell Adhesion Molecules; C092530:neurotrimin