Expression of sodium-glucose co-transporter and brush border disaccharidases in Giardia lamblia infected rat intestine.
The absorption of D-glucose and brush border membrane disaccharidases in the intestine of rat during infection by Giardia lamblia has been studied. The level of mRNA encoding Na+/glucose co-transporter (SGLT1) and brush border sucrase and lactase activities were also analyzed. At the peak of infection, i.e, day 7, 11 and 15 post-infection, there was a marked decrease in the signal of 4.5 kb and 2.8 kb mRNAs encoding SGTL1 compared to the controls. A similar decrease in sucrase and lactase mRNA's (6.5 kb and 6.8 kb respectively) was also observed under these conditions. This corresponds to observed decrease in the rate of Na(+)-dependent D-glucose uptake and low activities of brush border sucrase and lactase under these conditions. There was no change in Na(+)-independent D-glucose uptake in giardia infected rat intestine. These findings suggest that the down regulation of the expression of SGLT1 and brush border sucrase and lactase activities may be responsible for the observed malabsorption in G. lamblia infection.
Department of Experimental Medicine & Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh 160 012, India.
S
S
Mahmood
C P
CP
Sodhi
N K
NK
Ganguly
D000818:Animals; D004186:Disaccharidases / Q000096:biosynthesis*; D015971:Gene Expression Regulation, Enzymologic; D016829:Giardia lamblia / Q000378:metabolism*; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000469:parasitology; D043322:Lactase / Q000096:biosynthesis; D008871:Microvilli / Q000201:enzymology* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051247:Sodium-Glucose Transport Proteins / Q000378:metabolism*; D051269:Sodium-Glucose Transporter 1 / Q000378:metabolism*; D013393:Sucrase / Q000096:biosynthesis; D013997:Time Factors
D012333:RNA, Messenger; C495676:Slc5a1 protein, rat; D051247:Sodium-Glucose Transport Proteins; D051269:Sodium-Glucose Transporter 1; D004186:Disaccharidases; D043322:Lactase; D013393:Sucrase; D005947:Glucose