Influences of surface chemistry and swelling of salt-treated polyelectrolyte multilayers on migration of smooth muscle cells.
The cell migration plays a crucial role in a variety of physiological and pathological processes and can be regulated by the cell-substrate interactions. We found previously that the poly(sodium 4-styrenesulphonate) (PSS)/poly(diallyldimethylammonium) chloride (PDADMAC) multilayers post-treated in 1-5 M NaCl solutions result in continuous changes of their physico-chemical properties such as thickness, chemical composition, surface charge, swelling ratio and wettability. In this study, the responses of human smooth muscle cells (SMCs) on these salt-treated multilayers, particularly the governing factors of cellular migration that offer principles for designing therapeutics and implants, were disclosed. The cell migration rate was slowest on the 3 M NaCl-treated multilayers, which was comparable with that on tissue culture plates, but it was highest on 5 M NaCl-treated multilayers. To elucidate the intrinsic mechanisms, cell adhesion, proliferation, adhesion and related gene expressions were further investigated. The SMCs preferred to attach, spread and proliferate on the PSS-dominated surfaces with well-organized focal adhesion and actin fibres, especially on the 3 M NaCl-treated multilayers, while were kept round and showed low viability on the PDADMAC-dominated surfaces. The relative mRNA expression levels of adhesion-related genes such as fibronectin, laminin and focal adhesion kinase, and migration-related genes such as myosin IIA and Cdc42 were compared to explain the different cellular behaviours. These results reveal that the surface chemistry and the swelling of the salt-treated multilayers govern the cell migration behaviours.
MOE of Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
Lulu
L
Han
Zhengwei
Z
Mao
Jindan
J
Wu
Yuying
Y
Zhang
Changyou
C
Gao
D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002465:Cell Movement; D002478:Cells, Cultured; D022001:Focal Adhesions; D015870:Gene Expression; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology*; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods
D011095:Polyethylenes; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D013451:Sulfonic Acids; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D012965:Sodium Chloride; C077114:styrenesulfonic acid polymer