Production of bispecific antibodies: diabodies and tandem scFv.
Recombinant bispecific antibodies have many different applications; especially promising is their therapeutic potential due to their ability to retarget an effector molecule or a cell to a disease-related target structure. In the last years, many formats have been developed: two commonly used are the bispecific diabody and the tandem scFv. In this chapter, the cloning, bacterial production, purification, and characterization of the two antibody formats are described in detail.
Institute of Cell Biology and Immunology, University of Stuttgart, Stuttgart, Germany. nora.hornig@izi.uni-stuttgart.de
Nora
N
Hornig
Aline
A
Färber-Schwarz
D018033:Antibodies, Bispecific / Q000096:biosynthesis* / Q000302:isolation & purification; D001665:Binding Sites; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D057127:Single-Chain Antibodies / Q000096:biosynthesis* / Q000302:isolation & purification
D018033:Antibodies, Bispecific; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies