Short communication: isolation of a whey fraction rich in α-lactalbumin from skim milk using tangential flow ultrafiltration.
The objective of this research was to evaluate the potential for separating α-lactalbumin from pasteurized milk by using tangential flow membrane filtration. Filtration was carried out with a Purosep 7000 series membrane filtration unit (SmartFlow Technologies) with a regenerated cellulose membrane at 26°C and a transmembrane pressure of 186 kPa. The protein in the permeate was >80% α-lactalbumin, and this product could be used as a value-added ingredient in nutritional products.
Department of Food Science, University of Guelph, Guelph, Ontario, Canada, N1G2W1.
B
B
Holland
J
J
Kackmar
M
M
Corredig
D000818:Animals; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D007768:Lactalbumin / Q000302:isolation & purification*; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000379:methods*; D000067816:Whey Proteins
D008894:Milk Proteins; D000067816:Whey Proteins; D007768:Lactalbumin