Azorhizobium oxalatiphilum sp. nov., and emended description of the genus Azorhizobium.
A gram-negative, motile, non-spore-forming rod, designated NS12(T), was isolated from macerated petioles of Rumex sp. after enrichment with oxalate. On the basis of 16S rRNA gene sequence similarity, strain NS12(T) was phylogenetically related to the genera Azorhizobium and Xanthobacter in the class Alphaproteobacteria. Strain NS12(T) was most closely related to Azorhizobium doebereinerae BR 5401(T) and Azorhizobium caulinodans ORS 571(T) (98.3 and 97.3 % 16S rRNA gene sequence similarity, respectively). Membership of the genus Xanthobacter was excluded by phenotypic characterization. The whole-cell fatty acid compositions of the isolate was typical of members of the genus Azorhizobium with C18 : 1ω7c, cyclo-C19 : 0ω8c, 11-methyl-C18 : 1ω7c and C16 : 0 as the main components. The results of DNA-DNA hybridization and physiological tests allowed the genotypic and phenotypic differentiation of strain NS12(T) from the two members of the genus Azorhizobium. Therefore it is concluded that the isolate represents a novel species of the genus Azorhizobium, for which the name Azorhizobium oxalatiphilum sp. nov. is proposed. The type strain is NS12(T) ( = DSM 18749(T) = CCM 7897(T)). The description of the genus Azorhizobium is also emended.
Leibniz-Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7b, 30124 Braunschweig, Germany.
Elke
E
Lang
Leibniz-Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7b, 30124 Braunschweig, Germany.
Peter
P
Schumann
Philipps-Universität Marburg, Zellbiologie I, Karl von Frisch Straße 8, 35032 Marburg, Germany.
Susann
S
Adler
Leibniz-Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen, Inhoffenstraße 7b, 30124 Braunschweig, Germany.
Cathrin
C
Spröer
Mugla Sitki Kocman University, Egitim Fakültesi, TR-48170 Kötekli, Mugla, Turkey.
Nurettin
N
Sahin
D020583:Azorhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D031804:Rumex / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S