New anti-HIV aptamers based on tetra-end-linked DNA G-quadruplexes: effect of the base sequence on anti-HIV activity.
This communication reports on the synthesis and biophysical, biological and SAR studies of a small library of new anti-HIV aptamers based on the tetra-end-linked G-quadruplex structure. The new aptamers showed EC(50) values against HIV-1 in the range of 0.04-0.15 μM as well as affinities for the HIV-1 gp120 envelope in the same order of magnitude.
Dipartimento di Chimica delle Sostanze Naturali, Università degli Studi di Napoli Federico II, Via D. Montesano 49, I-80131 Napoli, Italy.
Valentina
V
D'Atri
Giorgia
G
Oliviero
Jussara
J
Amato
Nicola
N
Borbone
Stefano
S
D'Errico
Luciano
L
Mayol
Vincenzo
V
Piccialli
Shozeb
S
Haider
Bart
B
Hoorelbeke
Jan
J
Balzarini
Gennaro
G
Piccialli
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D054856:G-Quadruplexes; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D009841:Oligonucleotides / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
D052157:Aptamers, Nucleotide; D015699:HIV Envelope Protein gp120; D009841:Oligonucleotides; D011994:Recombinant Proteins; C514793:gp120 protein, Human immunodeficiency virus 1