Sphingobacterium thermophilum sp. nov., of the phylum Bacteroidetes, isolated from compost.
A Gram-negative bacterium, designated CKTN2(T), was isolated from compost. Cells of strain CKTN2(T) were strictly aerobic rods. The isolate grew at 20-50 °C (optimum 40-45 °C), but not below 15 °C or above 52 °C, and at pH 5.9-8.8 (optimum pH 7.0), but not below pH 5.4 or above pH 9.3. The DNA G+C content was 40.3 mol%. The predominant menaquinone was MK-7. The major fatty acids were iso-C15 : 0 (45.2 %), iso-C17 : 0 3-OH (11.1 %) and C18 : 0 (14.5 %). Analysis of the 16S rRNA gene sequence of strain CKTN2(T) revealed that it is a member of the genus Sphingobacterium and is most closely related to Sphingobacterium alimentarium DSM 22362(T) (93.2 % 16S rRNA gene sequence similarity). Strain CKTN2(T) could be distinguished from its closest phylogenetic relatives by different phenotypic characteristics. According to the phenotypic and genotypic characteristics, strain CKTN2(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium thermophilum sp. nov. is proposed. The type strain is CKTN2(T) ( = JCM 17858(T)  = KCTC 23708(T)).
Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
Shuhei
S
Yabe
Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
Yoshifumi
Y
Aiba
Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
Yasuteru
Y
Sakai
Hazaka Plant Research Center, Kennan Eisei Kogyo Co., Ltd, 44 Aza-Inariyama, Oaza-Ashitate, Murata-cho, Shibata-gun, Miyagi 989-1311, Japan.
Masaru
M
Hazaka
Department of Applied Material and Life Science, College of Engineering, Kanto Gakuin University, Rokkurahigashi, Kanazawa-ku, Yokohama 236-8501, Japan.
Kazuyoshi
K
Kawahara
Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113-0032, Japan.
Akira
A
Yokota
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D007564:Japan; D008372:Manure / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D005227:Fatty Acids; D008372:Manure; D012336:RNA, Ribosomal, 16S; D012987:Soil