Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice.
Fluorescence-activated cell sorting (FACS) provides a rapid means of isolating large numbers of fluorescently tagged cells from a heterogeneous mixture of cells. Collections of transgenic plants with cell type-specific expression of fluorescent marker genes such as green fluorescent protein (GFP) are ideally suited for FACS-assisted studies of individual cell types. Here we describe the use of Arabidopsis and rice enhancer trap lines with tissue-specific GFP expression patterns in the root to isolate specific cell types of root tissues using FACS. Additionally, protocols are provided to impose a ramped salinity stress for 48 h prior to cell sorting.
Australian Centre for Plant Functional Genomics, University of Adelaide, Glen Osmond, SA, Australia.
Aurelie
A
Evrard
Bastiaan O R
BO
Bargmann
Kenneth D
KD
Birnbaum
Mark
M
Tester
Ute
U
Baumann
Alexander A T
AA
Johnson
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D002469:Cell Separation; D005434:Flow Cytometry; D018525:Germination / Q000502:physiology; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011523:Protoplasts / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance / Q000502:physiology; D013312:Stress, Physiological