Exercise training prevents ecto-nucleotidases alterations in platelets of hypertensive rats.
In this study, we investigated the effect of 6 weeks of swimming training on the ecto-nucleotidase activities and platelet aggregation from rats that developed hypertension in response to oral administration of L-NAME. The rats were divided into four groups: control (n = 10), exercise (n = 10), L-NAME (n = 10), and exercise L-NAME (n = 10). The animals were trained five times per week in an adapted swimming system for 60 min with a gradual increase of the workload up to 5 % of animal's body weight. The results showed an increase in ATP, ADP, AMP, and adenosine hydrolysis, indicating an augment in NTPDase (from 35.3 ± 8.1 to 53.0 ± 15.1 nmol Pi/min/mg protein for ATP; and from 21.7 ± 7.0 to 46.4 ± 15.6 nmol Pi/min/mg protein for ADP as substrate), ecto-5'-nucleotidase (from 8.0 ± 5.7 to 28.1 ± 6.9 nmol Pi/min/mg protein), and ADA (from 0.8 ± 0.5 to 3.9 ± 0.8 U/L) activities in platelets from L-NAME-treated rats when compared to other groups (p < 0.05). A significant augment on platelet aggregation in L-NAME group was also observed. Exercise training was efficient in preventing these alterations in the exercise L-NAME group, besides showing a significant hypotensive effect. In conclusion, our results clearly indicated a protector action of moderate intensity exercise on nucleotides and nucleoside hydrolysis and on platelet aggregation, which highlights the exercise training effect to avoid hypertension complications related to ecto-nucleotidase activities.
Post-Graduation Program in Toxicological Biochemistry, Department of Chemistry, Center of Natural and Exact Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Andréia Machado
AM
Cardoso
Margarete Dulce
MD
Bagatini
Caroline Curry
CC
Martins
Fátima Hussein
FH
Abdalla
Daniela
D
Zanini
Roberta
R
Schmatz
Jessié
J
Gutierres
Victor Camera
VC
Pimentel
Gustavo
G
Thomé
Claudio Alberto Martins
CA
Leal
Juliano Marchi
JM
Vieira
Naiara
N
Stefanello
Fernando
F
da Silva Fiorin
Jucimara
J
Baldissareli
Luiz Fernando Freire
LF
Royes
Adriane Bello
AB
Klein
Vera Maria
VM
Morsch
Maria Rosa Chitolina
MR
Schetinger
D015720:5'-Nucleotidase / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D006868:Hydrolysis; D006973:Hypertension / Q000097:blood*; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D010805:Physical Conditioning, Animal; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D051381:Rats; D017208:Rats, Wistar
D009569:Nitric Oxide; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D019331:NG-Nitroarginine Methyl Ester