Determination of H(N),H          (α) and H          (N),C' coupling constants in           (13)C,           (15)N-labeled proteins.
Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the (3)J(H(N),H(α)) and (3)J(H(N),C') coupling constants in uniformly (13)C- and (15)N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method in HSQC-type spectra (Cavanagh et al., 1991; Palmer et al., 1991). In most cases, the two measured coupling constants unambiguously define the ϕ-angle for protein structure determination. The method is applied to uniformly (13)C, (15)N-labeled ribonuclease T(1).
Institut für Biophysikalische Chemie, Universität Frankfurt, Marie-Curie-Strasse 11, D-60439, Frankfurt, Germany.
R
R
Weisemann
H
H
Rüterjans
H
H
Schwalbe
J
J
Schleucher
W
W
Bermel
C
C
Griesinger