The evaluation of enteric viruses in asymptomatic free-ranging non-human primates (Alouatta guariba clamitans, Alouatta caraya, Callithrix spp., Callithrix penicillata, and Leontopithecus caissara) in southern Brazil.
BACKGROUND
This study evaluated the presence of rotavirus groups A, B, and C (RV-A, RV-B, and RV-C), sapovirus (SaV), and norovirus (NoV) in asymptomatic non-human primates (NHP).


METHODS
Fecal samples were collected from 19 recently captured (Red-howler, Alouatta guariba clamitans, n = 18; Howler, Alouatta caraya, n = 1) and 43 free-ranging NHP (Marmosets, Callithrix spp., Callithrix penicillata, n = 30; Black-faced lion tamarin, Leontopithecus caissara, n = 12, Red-howler, Alouatta guariba clamitans, n = 1) that were maintained in southern Brazil without manifestation of diarrhea. Screening was performed by a combination of silver-stained polyacrylamide gel electrophoresis (ss-PAGE) and RT-PCR analyses.


RESULTS
All samples were negative for RV-A, RV-B, RV-C, SaV, and NoV by both assays.


CONCLUSIONS
The negative results obtained might be due to the absence of clinical manifestations of disease in the population of NHP evaluated.
Graduate Program in Pharmaceutical Sciences, Universidade Federal do Paraná, Curitiba, Brazil. fabimenoncin@gmail.com
Fabiana
F
Souza
Amauri A
AA
Alfieri
Alice F
AF
Alfieri
Elis
E
Lorenzetti
Selwyn A
SA
Headley
Fernando
F
Passos
Thiago
T
Silvestre
Luciana
L
Zago
Viviane
V
Mottin
Fabiano
F
Montiani-Ferreira
Rogério
R
Lange
Walfrido
W
Svoboda
Eliane
E
Gomes
D000508:Alouatta / Q000821:virology*; D000818:Animals; D001938:Brazil; D002144:Callithrix / Q000821:virology*; D005243:Feces / Q000821:virology*; D051039:Leontopithecus / Q000821:virology*; D029322:Norovirus; D012401:Rotavirus; D029341:Sapovirus