Enhanced expression of heterotrimeric GTP-binding protein subunits in Zajdela ascitic hepatoma.
G protein beta subunit (Gbeta2) is over-expressed in Zajdela ascitic hepatoma (ZAH), a rat ascitic tumour, at mRNA as well as protein levels. Nuclear run-off transcription analysis suggests that the expression of Gbeta2 in ZAH is regulated mainly at transcriptional as well as post-transcriptional levels. Galphai3 is also over-expressed in ZAH. No amplification or any change in the organization of the genes for GBbeta2 or Galphai3 was observed. It is possible that the over-expression of G protein subunits in ZAH could provide proliferative advantage to the cells by virtue of their effects on second messenger systems.
Center for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
S K
SK
Sharma
M R
MR
Das
D000818:Animals; D001201:Ascites / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D019281:Dimerization; D019204:GTP-Binding Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D014158:Transcription, Genetic
D012333:RNA, Messenger; D019204:GTP-Binding Proteins