Serum tolerance and endosomal escape capacity of histidine-modified pDNA-loaded complexes based on polyamidoamine dendrimer derivatives.
Aiming to aid polyamidoamine (PAMAM, generation 4, PG4) to overcome gene delivery barriers like extrinsic serum inhibition, intrinsic cytotoxicity and lysosome digestion, histidine motifs modified PAMAM was prepared. The histidine activated PAMAM generation 4 (HPG4) was synthesized via aminolysis reaction and characterized by 1H NMR spectrum and MALDI-TOF-MS. Cytotoxicity profiles of HPG4 on MD-MB-231 cells were significantly improved in the form of polymer and polymer/DNA complexes comparing to PG4. The luciferase protein expression level of HPG4 was 20-, 2.7- and 1.2- fold higher than that of PG4, SuperFect and PEI 25k. Most importantly, flow cytometry and gene transfection studies showed that histidine motifs of HPG4 not only acted as enhancer for faster cellular uptake, but also played an important role on enhancing serum tolerance of the system on cellular uptake and transfection. Among the serum concentrations of 10%-50%, HPG4 showed 10-100 folds higher transfection efficiency than PG4. Intracellular fate observation conducted by confocal microscope provided visual and quantitative evidence that endsomal escape efficiency of HPG4 system was higher than that of PG4. Lastly, the endosomal escape mechanism of HPG4 system was analyzed by endosome destabilization and proton pump inhibition treatment. Collectively, compared to PG4/pDNA, HPG4/pDNA showed improvement on cellular uptake, serum tolerance, cytotoxicity profile, and endosomal escape.
School of Pharmaceutical Sciences and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
Yuting
Y
Wen
Zhenhuan
Z
Guo
Zhuo
Z
Du
Rong
R
Fang
Hongmei
H
Wu
Xin
X
Zeng
Chi
C
Wang
Min
M
Feng
Shirong
S
Pan
D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D050091:Dendrimers / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D011992:Endosomes / Q000378:metabolism; D005260:Female; D006639:Histidine / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D006801:Humans; D049410:Luciferases, Renilla / Q000235:genetics; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D048790:Renilla / Q000235:genetics; D044967:Serum / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection
D050091:Dendrimers; C531249:Poly(amidoamine); D011073:Polyamines; D006639:Histidine; D004247:DNA; D049410:Luciferases, Renilla