Mammalian cell surface display of full length IgG.
Display technology has been developed and widely used in antibody screening and selecting. While phage can only display antibody fragments, mammalian cells can display not only fragments but full-length antibodies. Here we described the display of full length antibody on the surface of 293 cells. Both heavy chain and light chain genes were cloned in a single mammalian expression vector containing dual mammalian expression cassettes. While transfected into 293 cells of the vector, both heavy and light chains were expressed. With the help of transmembrane domain of platelet-derived growth factor receptor (PDGFR-TM) fused at the 3'-end of heavy chain in frame, expressed full-length antibodies were displayed on the cell surface and can be easily detected and analyzed by flow cytometry.
Antivirus Research Center, School of Pharmaceutical Science, Southern Medical University, Guangzhou, China.
Chen
C
Zhou
Wenyan David
WD
Shen
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D061505:Cell Surface Display Techniques / Q000379:methods*; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008322:Mammals / Q000378:metabolism*; D013194:Staining and Labeling; D013223:Statistics as Topic; D014162:Transfection
D007074:Immunoglobulin G