Activation of tobacco leaf polyphenol oxidase by sodium dodecyl sulfate.
The effect of sodium dodecyl sulfate (SDS) on purified tobacco leaf PPO (PPO II) was investigated at various pHs and temperatures. SDS increased the activity of PPO II due to the formation of SDS-PPO II complex, leading to conformational changes, thus making access to active center easier. The relationship between the activity and the molar ratio of SDS-PPO II to PPO II showed that the critical point reached a plateau of activity at the molar ratio of about 1.2. The pH had a significant effect on interaction between SDS and PPO II, as compared to PPO II. The optimum catalytic temperature of the complex rose by 10 degrees C, suggesting that stabilization of the structure had been improved by the formation of complex.
Department of Chemistry, University of Science and Technology of China, Hefei, 230026, P. R. China.
Hui
H
Jiang
Chunhua
C
Shi
Yongshu
Y
Xie
Xiaolong
X
Xu
Qingliang
Q
Liu
D004156:Catechol Oxidase / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D018515:Plant Leaves / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013696:Temperature; D014026:Nicotiana / Q000201:enzymology*
D012967:Sodium Dodecyl Sulfate; D004156:Catechol Oxidase