Composition and antipathogenic activities of the twig essential oil of Chamaecyparis formosensis from Taiwan.
In this study, antipathogenic activities of the twig essential oil and its constituents from Chamaecyparis formosensis Matsum were evaluated in vitro against six plant pathogenic fungi. The essential oil from the fresh twigs was isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. Twenty-five compounds were identified, representing 98.9% of the oil. The main components were beta-eudesmol (25.1%), tau-muurolol (21.6%), elemol (15.0%), totarol (14.9%), and alpha-cadinol (12.4%). The twig oil (500 mcirog/mL) showed growth inhibitory activity against the phytopathogenic fungi, Fusarium oxysporum, Pestalotiopsis funereal, and Ganoderma austral, with antifungal indices of 92.7%, 71.1%, and 87.7%, respectively. In addition, the oil suppressed totally the growth of Rhizoctonia solani, Colletotrichum gloeosporioides, and Fusarium solani. In order to ascertain the source compounds of these antipathogenic activities, the main components were individually evaluated. Tau-Muurolol and alpha-cadinol exhibited excellent activity against F. oxysporum, R. solani, C. gloeosporioides, and F. solani, with IC50 < 50 microg/mL. These compounds also efficiently inhibited the mycelial growths of P. funereal and G. austral. Thus, alpha-cadinol and tau-muurolol could be considered as potential natural fungicides for controlling fungal pathogens and worth.
Division of Wood Cellulose, Taiwan Forestry Research Institute, Taipei, Taiwan 100.
Chen-Lung
CL
Ho
Kuo-Feng
KF
Hua
Kuan-Ping
KP
Hsu
Eugene I-chen
EI
Wang
Yu-Chang
YC
Su
D000935:Antifungal Agents / Q000737:chemistry* / Q000494:pharmacology*; D029780:Chamaecyparis / Q000737:chemistry*; D020231:Colletotrichum / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects; D032902:Ganoderma / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D012232:Rhizoctonia / Q000187:drug effects; D013624:Taiwan
D000935:Antifungal Agents; D009822:Oils, Volatile