Proteomic analysis of tomato (Solanum lycopersicum) secretome.
In fleshy fruits, fruit texture features are mainly related to chemical and mechanical properties of the cell wall. The description of tomato fruit cell wall proteome is a first step in the process of linking tomato genetic variability to fruit texture phenotypes. In this study, the proteome of 3 ripe tomato fruit lines with contrasted texture traits were studied. Weakly bound and soluble proteins were extracted from cell wall of the three cultivars using both destructive and non-destructive methods, respectively. Wall proteins were separated on 1D-PAGE, bands were excised and identified by LC-MS/MS. The software SignalP which searches for the leader peptide was used to discriminate between protein with or without signal peptide. In combine, seventy-five different cell wall proteins were recorded for both weakly bound and soluble cell wall fractions. The major identified functions included several proteins acting on polysaccharides, proteins involved in "lipid metabolism", proteins having interacting domain, "oxido-reductases" and "proteases" whose putative roles in ripe fruit cell wall is discussed. Several proteins with no obvious signal peptide, however, with accumulating supportive evidences to be bona fide wall proteins, were also identified. Some variations in protein repertories were observed among the lines, demonstrating the possibility to characterize cell wall protein genetic variability by such in muro proteome analyses.
Unité de Génétique et Amélioration des Fruits et Légumes, INRA, BP 94, 84143 Montfavet, France. ehkonozy@yahoo.com
Emadeldin H E
EH
Konozy
Hélène
H
Rogniaux
Mathilde
M
Causse
Mireille
M
Faurobert
D002473:Cell Wall / Q000378:metabolism; D002853:Chromatography, Liquid; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D005638:Fruit / Q000235:genetics / Q000378:metabolism*; D014644:Genetic Variation; D005838:Genotype; D018551:Solanum lycopersicum / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D010940:Plant Proteins / Q000302:isolation & purification* / Q000378:metabolism; D020543:Proteome; D040901:Proteomics; D013045:Species Specificity; D053719:Tandem Mass Spectrometry
D010940:Plant Proteins; D020543:Proteome