Neuroprotective activity of lavender oil on transient focal cerebral ischemia in mice.
The air-dried aerial parts of Lavandula angustifolia Mill, a traditional Uygur herbal drug, is used as resuscitation-inducing therapy to treat neurodisfunctions, such as stroke. This study was designed to assess the neuroprotective effects of lavender oil against ischemia/reperfusion (IR) injury in mice. Focal cerebral ischemia was induced by the intraluminal occlusion method with a nylon string. The neurodysfuntion was evaluated by neurological deficit and the infarct area was showed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. The histopathological changes were observed by hematoxylin and eosin staining. The levels of mitochondria-generated reactive oxygen species (ROS), malondialdehyde (MDA) and carbonyl, the ratio of reduced glutathione (GSH)/glutathione disulfide (GSSG), the activities of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) in brain tissue were measured to estimate the oxidative stress state. Neurological deficit, infarct size, histopathology changes and oxidative stress markers were evaluated after 22 h of reperfusion. In comparison with the model group, treatment with lavender oil significantly decreased neurological deficit scores, infarct size, the levels of MDA, carbonyl and ROS, and attenuated neuronal damage, upregulated SOD, CAT, GSH-Px activities and GSH/GSSG ratio. These results suggested that the neuroprotective effects of lavender oil against cerebral ischemia/reperfusion injury may be attributed to its antioxidant effects.
Qianfoshan Hospital of Shandong Province, Jinan 250005, China.
Dong
D
Wang
Xuan
X
Yuan
Ting
T
Liu
Liangliang
L
Liu
Yanli
Y
Hu
Zhenhua
Z
Wang
Qiusheng
Q
Zheng
D000818:Animals; D000975:Antioxidants; D001921:Brain / Q000187:drug effects / Q000473:pathology; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D027523:Lavandula / Q000737:chemistry; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D009822:Oils, Volatile / Q000008:administration & dosage*; D010936:Plant Extracts / Q000235:genetics; D010938:Plant Oils / Q000008:administration & dosage*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D018696:Neuroprotective Agents; D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; C045718:lavender oil