The effect of sebocytes cultured from nevus sebaceus on hair growth.
Sebaceous glands are known to affect hair growth. Nevus sebaceus, a sebaceous gland hamartomas, presents as hairless patches. In this study, cultures of nevus sebaceus sebocytes (NSS) and normal scalp hair follicle sebocytes (NS) were used in performance of microarray, RT-PCR, western blot assay and immunofluorescence staining. NSS- and NS-conditioned media were also added to the culture of outer root sheath cells (ORSCs), dermal papilla cells (DPCs) or normal scalp hair follicle sebocytes. Results of this study showed a decrease in the survival rate of ORSCs and DPCs and hair growth in the NSS-conditioned medium-treated group, compared with the control and NS-conditioned medium-treated groups. An increase in expression of fibroblast growth factor (FGF)-5, Dickkopf-1 and inflammatory cytokines and a decrease in expression of Wnt10b and Lef1 were observed. In conclusion, NSS showed an increase in expression of hair growth-suppressing bioactive factors, including FGF-5, and a decrease in expression of hair growth-stimulating factors.
Weon Ju
WJ
Lee
Hyun Wuk
HW
Cha
Hyun Jung
HJ
Lim
Seok-Jong
SJ
Lee
Do Won
DW
Kim
D017077:Culture Media, Conditioned; D051521:Fibroblast Growth Factor 5 / Q000235:genetics; D015870:Gene Expression; D006197:Hair / Q000254:growth & development*; D018859:Hair Follicle / Q000378:metabolism / Q000473:pathology; D006801:Humans; D009506:Nevus; D009508:Nevus, Pigmented / Q000378:metabolism / Q000473:pathology*; D012626:Sebaceous Gland Neoplasms / Q000378:metabolism / Q000473:pathology*; D012627:Sebaceous Glands / Q000378:metabolism / Q000473:pathology*; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; C496449:FGF5 protein, human; D051521:Fibroblast Growth Factor 5