Emergence of carbapenem-resistant OXA-48 carbapenemase-producing Enterobacteriaceae in Tunisia.
We screened 21 extended spectrum β-lactamase-producing Enterobacteriaceae with reduced susceptibility to carbapenems for carbapenemase production. Five strains (four Klebsiella pneumoniae and one Citrobacter freundii) showed carbapenemase production, which was identified as OXA-48. The bla(OXA-48) gene was detected on ~54 kb plasmids belonging to IncA/C in one case. Two isolates harboured IS1999, which is involved in bla(OXA-48) mobilization. Carbapenem resistance in enterobacteria should be regarded as an emerging clinical problem in our hospital and necessitates rigorous surveillance in order to limit its spread.
Laboratoire de Bactériologie Virologie, Hôpital Charles Nicolle, Boulevard 9 Avril, 1006, Tunis, Tunisia.
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Saïdani
Laboratoire de Bactériologie Virologie, Hôpital Charles Nicolle, Boulevard 9 Avril, 1006, Tunis, Tunisia.
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Hammami
Laboratoire de Bactériologie Virologie, Hôpital Charles Nicolle, Boulevard 9 Avril, 1006, Tunis, Tunisia.
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Kammoun
Laboratoire de Bactériologie Virologie, Hôpital Charles Nicolle, Boulevard 9 Avril, 1006, Tunis, Tunisia.
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Slim
Laboratoire de Bactériologie Virologie, Hôpital Charles Nicolle, Boulevard 9 Avril, 1006, Tunis, Tunisia.
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Boutiba-Ben Boubaker
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D015780:Carbapenems / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D014416:Tunisia; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase; C108449:oxacillinase