Molecular characterization of  Corynebacterium pseudotuberculosis isolated from goats using ERIC-PCR.
Corynebacterium pseudotuberculosis, the infectious agent of caseous  lymphadenitis (CLA), is responsible for substantial economic losses in goat and  sheep production. Molecular characterization of C. pseudotuberculosis isolates  by enterobacterial repetitive intergenic consensus (ERIC)-PCR has shown  promising results in genotyping strains isolated from sheep with CLA. We evaluated  the genetic diversity of C. pseudotuberculosis isolates collected from  the Sertão region of the Pernambuco (PE) State, Brazil, and investigated the potential  of ERIC-PCR as a tool for the molecular typing of strains of C.  pseudotuberculosis isolated from goats. Thirty-two C. pseudotuberculosis strains isolated from goats in the municipalities of Floresta and Ibimirim,  PE, C. pseudotuberculosis type strain ATCC 19410, the 1002 vaccine  strain, and a field isolate of Rhodococcus equi were fingerprinted  using the primers ERIC-1R and ERIC-2 and the primer pair ERIC- 1R+ERIC-2. Using  100% similarity as the cutoff, 8, 10, and 7 genotypes were obtained with  ERIC-1-PCR, ERIC-2-PCR, and ERIC-1+2-PCR, respectively. The Hunter-Gaston  discriminatory index calculated for the ERIC-1-PCR was 0.75. The index for the  ERIC-2-PCR was 0.88, and the index for the ERIC-1+2-PCR was 0.79. Among goat  isolates of C. pseudotuberculosis, three, two and four genotypes (found  by ERIC-1-PCR, ERIC-2-PCR, and ERIC-1+2-PCR, respectively) had been previously  described among sheep isolates from Minas Gerais State, Brazil. These results  showed that ERIC-PCR has good discriminatory power and typeability, making it  a useful tool for discrimination among C. pseudotuberculosis isolates  from goats.
Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
E M S
EM
Dorneles
J A
JA
Santana
G I
GI
Andrade
E L S
EL
Santos
A S
AS
Guimarães
R A
RA
Mota
A S
AS
Santos
A
A
Miyoshi
V
V
Azevedo
A M G
AM
Gouveia
A P
AP
Lage
M B
MB
Heinemann
D000818:Animals; D001938:Brazil; D016000:Cluster Analysis; D016384:Consensus Sequence / Q000235:genetics*; D016925:Corynebacterium pseudotuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D021901:DNA, Intergenic / Q000235:genetics*; D004755:Enterobacteriaceae / Q000235:genetics; D006041:Goats / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D021901:DNA, Intergenic