Prostatic fibrosis is associated with lower urinary tract symptoms.
OBJECTIVE
Current therapies for male lower urinary tract symptoms secondary to prostate enlargement prevent hormonal effects on prostate growth and inhibit smooth muscle contraction to ease bladder neck and urethral pressure. However, lower urinary tract symptoms can be refractory to these therapies, suggesting that additional biological processes not addressed by them may also contribute to lower urinary tract symptoms. Aging associated fibrotic changes in tissue architecture contribute to dysfunction in multiple organ systems. Thus, we tested whether such changes potentially have a role in impaired urethral function and perhaps in male lower urinary tract symptoms.


METHODS
Periurethral tissues were obtained from a whole prostate ex vivo and from 28 consecutive men treated with radical prostatectomy. Lower urinary tract symptoms were assessed using the American Urological Association symptom index. Prostate tissues were subjected to mechanical testing to assess rigidity and stiffness. Fixed sections of these tissues were evaluated for collagen and elastin content, and glandularity to assess fibrosis. Statistical analysis included the Student t test and calculation of Pearson correlation coefficients to compare groups.


RESULTS
Periurethral prostate tissues demonstrated nonlinear viscoelastic mechanical behavior. Tissue from men with lower urinary tract symptoms was significantly stiffer (p = 0.0016) with significantly higher collagen content (p = 0.0038) and lower glandularity than that from men without lower urinary tract symptoms (American Urological Association symptom index 8 or greater vs 7 or less).


CONCLUSIONS
Findings show that extracellular matrix deposition and fibrosis characterize the periurethral prostate tissue of some men with lower urinary tract symptoms. They point to fibrosis as a factor contributing to lower urinary tract symptom etiology.
Department of Mechanical Engineering, University of Michigan, Ann Arbor, Michigan, USA.
Jinjin
J
Ma
Mehrnaz
M
Gharaee-Kermani
Lakshmi
L
Kunju
John M
JM
Hollingsworth
Jeremy
J
Adler
Ellen M
EM
Arruda
Jill A
JA
Macoska
D000368:Aged; D005355:Fibrosis / Q000150:complications; D006801:Humans; D059411:Lower Urinary Tract Symptoms / Q000209:etiology*; D008297:Male; D011467:Prostate / Q000473:pathology*; D014521:Urethra