Mutational properties of amino acid residues: implications for evolvability of phosphorylatable residues.
As François Jacob pointed out over 30 years ago, evolution is a tinkering process, and, as such, relies on the genetic diversity produced by mutation subsequently shaped by Darwinian selection. However, there is one implicit assumption that is made when studying this tinkering process; it is typically assumed that all amino acid residues are equally likely to mutate or to result from a mutation. Here, by reconstructing ancestral sequences and computing mutational probabilities for all the amino acid residues, we refute this assumption and show extensive inequalities between different residues in terms of their mutational activity. Moreover, we highlight the importance of the genetic code and physico-chemical properties of the amino acid residues as likely causes of these inequalities and uncover serine as a mutational hot spot. Finally, we explore the consequences that these different mutational properties have on phosphorylation site evolution, showing that a higher degree of evolvability exists for phosphorylated threonine and, to a lesser extent, serine in comparison with tyrosine residues. As exemplified by the suppression of serine's mutational activity in phosphorylation sites, our results suggest that the cell can fine-tune the mutational activities of amino acid residues when they reside in functional protein regions.
Cellular Signal Integration Group (C-SIG), Center for Biological Sequence Analysis (CBS), Department of Systems Biology, Technical University of Denmark (DTU), DK-2800 Lyngby, Denmark.
Pau
P
Creixell
Erwin M
EM
Schoof
Chris Soon Heng
CS
Tan
Rune
R
Linding
D000465:Algorithms; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000596:Amino Acids / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D000818:Animals; D001665:Binding Sites; D055598:Chemical Phenomena; D019295:Computational Biology / Q000379:methods; D017124:Conserved Sequence; D019143:Evolution, Molecular; D006801:Humans; D009154:Mutation; D010750:Phosphoproteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000502:physiology; D010766:Phosphorylation; D010802:Phylogeny; D012641:Selection, Genetic; D016415:Sequence Alignment
D000596:Amino Acids; D010750:Phosphoproteins