aroA deleted Bordetella bronchiseptica inspiring robust mucosal immune response and provide full protection against intranasal challenge.
Bordetella bronchiseptica is a Gram-negative respiratory pathogen responsible for atrophic rhinitis and bronchopneumonia in swine. Several vaccines aimed at preventing B. bronchiseptica have been used, but a safe and efficient live vaccine for use in piglets remains elusive. In this study, we constructed an aroA-deleted B. bronchiseptica strain (QH0814) and evaluated its safety and protective efficiency in piglets. Lung lesion scores in QH0814-immunized piglets post-challenge were significantly lower than those in piglets immunized with the parent strain (P<0.05). Immunization with QH0814 induced a vigorous immune response, especially at the mucosal surface of the respiratory tract. IgA titers in bronchoalveolar lavage fluid (BALF) and serum were significantly higher in the QH0814-immunized group compared to the inactivated-vaccine-immunized group. Piglets immunized with QH0814 were better protected than those in the inactivated-vaccine and negative control groups. The clinical symptoms, histopathological changes and immune responses elicited in the piglets were recorded. The results of this study suggest that QH0814 was able to confer complete protection against B. bronchiseptica infection and could thus be used as a candidate attenuated live vaccine against B. bronchiseptica in piglets.
Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Qian
Q
Zhang
Ruiming
R
Hu
Junyong
J
Hu
Hua
H
He
Xibiao
X
Tang
Meilin
M
Jin
Huanchun
H
Chen
Bin
B
Wu
D000281:Administration, Intranasal / Q000662:veterinary; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001428:Bacterial Vaccines / Q000276:immunology* / Q000627:therapeutic use; D001885:Bordetella Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016950:Bordetella bronchiseptica / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology / Q000382:microbiology; D005260:Female; D017353:Gene Deletion; D056724:Immunity, Humoral / Q000276:immunology; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood / Q000276:immunology; D008168:Lung / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D020545:Respiratory Mucosa / Q000276:immunology / Q000382:microbiology; D012222:Rhinitis, Atrophic / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary; D013552:Swine / Q000276:immunology / Q000382:microbiology; D013553:Swine Diseases / Q000276:immunology / Q000382:microbiology* / Q000517:prevention & control; D014613:Vaccines, Attenuated
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D007070:Immunoglobulin A; D014613:Vaccines, Attenuated