Investigation of glandular trichome proteins in Artemisia annua L. using comparative proteomics.
Glandular secreting trichomes (GSTs) are called biofactories because they are active in synthesizing, storing and secreting various types of plant secondary metabolites. As the most effective drug against malaria, artemisinin, a sesquiterpene lactone is derived from GSTs of Artemisia annua. However, low artemisinin content (0.001%~1.54% of dry weight) has hindered its wide application. We investigate the GST-expressed proteins in Artemisia annua using a comparative proteomics approach, aiming for a better understanding of the trichome proteome and arteminisin metabolism. 2D-electrophoresis was employed to compare the protein profiles of GSTs and leaves. More than 700 spots were resolved for GSTs, of which ∼93 non-redundant proteins were confidently identified by searching NCBI and Artemisia EST databases. Over 70% of these proteins were highly expressed in GTSs. Functional classification of these GSTs enriched proteins revealed that many of them participate in major plant metabolic processes such as electron transport, transcription and translation.
School of Life Sciences and State Kay Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, People's Republic of China.
Ting
T
Wu
Yejun
Y
Wang
Dianjing
D
Guo
D031172:Artemisia annua / Q000378:metabolism*; D037621:Artemisinins / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007783:Lactones / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D008954:Models, Biological; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D015854:Up-Regulation
D037621:Artemisinins; D007783:Lactones; D010940:Plant Proteins; D020543:Proteome; C031327:artemisinin