An automated two-phase system for hydrogel microbead production.
Polymeric beads have been used for protection and delivery of bioactive materials, such as drugs and cells, for different biomedical applications. Here, we present a generic two-phase system for the production of polymeric microbeads of gellan gum or alginate, based on a combination of in situ polymerization and phase separation. Polymer droplets, dispensed using a syringe pump, formed polymeric microbeads while passing through a hydrophobic phase. These were then crosslinked, and thus stabilized, in a hydrophilic phase as they crossed through the hydrophobic-hydrophilic interface. The system can be adapted to different applications by replacing the bioactive material and the hydrophobic and/or the hydrophilic phases. The size of the microbeads was dependent on the system parameters, such as needle size and solution flow rate. The size and morphology of the microbeads produced by the proposed system were uniform, when parameters were kept constant. This system was successfully used for generating polymeric microbeads with encapsulated fluorescent beads, cell suspensions and cell aggregates proving its ability for generating bioactive carriers that can potentially be used for drug delivery and cell therapy.
3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Taipas, 4806-909 Guimarães, Portugal.
Daniela F
DF
Coutinho
Amir F
AF
Ahari
Nezamoddin N
NN
Kachouie
Manuela E
ME
Gomes
Nuno M
NM
Neves
Rui L
RL
Reis
Ali
A
Khademhosseini
D000464:Alginates / Q000737:chemistry; D000818:Animals; D001331:Automation; D045744:Cell Line, Tumor; D064987:Cell- and Tissue-Based Therapy; D004337:Drug Carriers / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D008863:Microspheres; D041681:NIH 3T3 Cells; D011108:Polymers / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry
D000464:Alginates; D004337:Drug Carriers; D005456:Fluorescent Dyes; D006603:Hexuronic Acids; D011108:Polymers; D011135:Polysaccharides, Bacterial; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C048288:gellan gum; D020723:Glucuronic Acid