Passive immunization with tenascin-R (TN-R) polyclonal antibody promotes axonal regeneration and functional recovery after spinal cord injury in rats.
Tenascin-R (TN-R) is a neural specific protein and an important molecule involved in inhibition of axonal regeneration after spinal cord injury (SCI). Here we report on rabbit-derived TN-R polyclonal antibody, which acts as a TN-R antagonist with high titer and high specificity, promoted neurite outgrowth and sprouting of rat cortical neurons cultured on the inhibitory TN-R substrate in vitro. When locally administered into the lesion sites of rats received spinal cord dorsal hemisection, these TN-R antibodies could significantly decrease RhoA activation and improve functional recovery from corticospinal tract (CST) transection. Thus, passive immunotherapy with specific TN-R antagonist may represent a promising repair strategy following acute SCI.
Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Jian
J
You
Sun-Quan
SQ
Hong
Mao-Ying
MY
Zhang
Hai-Lin
HL
Zhao
Tian-Zhu
TZ
Liu
Hong-Long
HL
Zhou
Ying-Qian
YQ
Cai
Zhi-Min
ZM
Xu
Yang
Y
Guo
Xiao-Dan
XD
Jiang
Ru-Xiang
RX
Xu
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000494:pharmacology* / Q000627:therapeutic use; D001369:Axons / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D006614:Hindlimb / Q000503:physiopathology; D007116:Immunization, Passive; D009043:Motor Activity; D009416:Nerve Regeneration; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000276:immunology / Q000503:physiopathology / Q000628:therapy*; D019063:Tenascin / Q000037:antagonists & inhibitors* / Q000276:immunology; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
D000906:Antibodies; D019063:Tenascin; C074638:tenascin R; D020742:rhoA GTP-Binding Protein