Proteomes of umbilical vein and microvascular endothelial cells reflect distinct biological properties and influence immune recognition.
Human umbilical vein endothelial cells (HUVEC) are widely used as a source of endothelial cells (EC). However, HUVEC characteristics cannot be extrapolated to other types of EC, particularly microvascular ECs. Our objective was to compare the proteomes of microvascular ECs and HUVEC. Proteomes of HUVEC and human microvascular pulmonary EC (HMVEC-P) and dermal EC (HMVEC-D) from healthy Caucasian donors were compared by 2D DIGE and MS. Fatty acid binding proteins 4 and 5 were among the 159 and 30 proteins spots found to have at least twofold change in expression between HUVEC and HMVEC-D and between HUVEC and HMVEC-P samples, respectively. Eight protein spots showed twofold changed expression between HMVEC-D and HMVEC-P samples. Ingenuity® analysis revealed that proteins differentially expressed between HUVEC and HMVEC-D samples interact with retinoic acid. In vitro tubulogenesis assays showed a differential effect of retinoic acid between HUVEC and HMVEC. Moreover, serum IgG from patients with a rare vascular disease, systemic sclerosis, showed distinct reactivity profiles in HUVEC and HMVEC-D protein extracts. The proteome profiles of HUVEC and microvascular EC differ noticeably, which reflects distinct biological properties and influence immune recognition.
INSERM U1016, Institut Cochin, Paris, France.
Hanadi
H
Dib
Philippe
P
Chafey
Guilhem
G
Clary
Christian
C
Federici
Morgane
M
Le Gall
Julie
J
Dwyer
Julie
J
Gavard
Nicolas
N
Tamas
Guillaume
G
Bussone
Cédric
C
Broussard
Luc
L
Camoin
Véronique
V
Witko-Sarsat
Mathieu C
MC
Tamby
Luc
L
Mouthon
D020405:Dermis / Q000098:blood supply; D015180:Electrophoresis, Gel, Two-Dimensional; D042783:Endothelial Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D020869:Gene Expression Profiling; D006262:Health; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008168:Lung / Q000098:blood supply; D055806:Microvessels / Q000166:cytology*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D020543:Proteome / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014019:Tissue Donors; D014212:Tretinoin / Q000494:pharmacology
D020543:Proteome; D014212:Tretinoin