Development of a pH-activatable fluorescent probe and its application for visualizing cellular pH change.
Novel pH-activatable fluorescent probes with various pK(a) values have been developed utilizing 4-nitro-benzo[1,2,5]oxadiazole derivatives (NBD) as fluorophores and piperazine moieties as proton receptors. Under acidic conditions, probe FoPz displayed significant fluorescent enhancement of about 30 fold with a pK(a) value of 5.70 and it responds rapidly and sensitively to intracellular pH distributions and cellular pH fluctuations.
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, PR China.
Rong
R
Huang
Shengyong
S
Yan
Xiaolong
X
Zheng
Fengling
F
Luo
Minggang
M
Deng
Boshi
B
Fu
Yijie
Y
Xiao
Xi
X
Zhao
Xiang
X
Zhou
D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D010069:Oxadiazoles / Q000737:chemistry; D011522:Protons
D005456:Fluorescent Dyes; D010069:Oxadiazoles; D011522:Protons