A new strategy employed for identification of sweet  orange cultivars with RAPD markers.
We optimized RAPD techniques by increasing the length  of RAPD primers and performing a strict screening of PCR annealing temperature  to distinguish 60 sweet orange cultivars from the Research Institute of  Pomology at the Chinese Academy of Agricultural Sciences. A new approach called  cultivar identification diagram (CID) was used to improve the efficiency of  RAPD markers for cultivar identification. Thirteen effective primers were first  screened from 54 RAPD arbitrary 11-mer primers based on their amplification  products and amplified polymorphic bands; they were then used for PCR  amplification of all 60 cultivars. All cultivars were manually and completely  separated by the polymorphic bands appearing in DNA fingerprints from 13  primers; a CID of the 60 sweet orange cultivars was then constructed. This CID  separated all the cultivars from each other, based on the polymorphic bands;  the corresponding primers were marked in the correct positions on the sweet  orange CID. The CID strategy facilitates the identification of fruit cultivars  with DNA markers. This CID of sweet orange cultivars will be very useful for  the protection of cultivar rights and for early identification of seedlings in  the nursery industry.
College of Horticulture, Nanjing Agricultural University, Nanjing, P.R. China.
X
X
Sun
Q
Q
Mu
D
D
Jiang
C
C
Wang
X C
XC
Wang
J G
JG
Fang
D032084:Citrus sinensis / Q000145:classification* / Q000235:genetics*; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers