Age-related  changes in mitochondrial UCP, ANT and COX III gene expression in the breast  muscle of quails (Coturnix coturnix japonica).
Efficient  conversion of food into body mass has been associated with altered gene  expression of some proteins of the electron transport chain. We evaluated the  effect of age on mRNA expression of uncoupling protein, adenine nucleotide  translocase and cytochrome c oxidase complex III in quails. One-day-old broiler  quails were sacrificed after 7, 14, 21, or 28 days. Six quails of each age were  slaughtered by cervical dislocation. Total RNA was extracted from the breast  muscle and reverse transcribed into cDNA. Real-time PCR analysis was performed  using specific primers for the genes. As the quails aged, there was reduced  expression of all three genes. The greatest reduction when comparing 7- and  28-day-old birds was observed in the uncoupling protein mRNA levels (67%),  followed by adenine nucleotide translocase (56%) and cytochrome c oxidase  complex III (30%). We conclude that expression of some mitochondrial genes of  the electron transport chain changes with age in quails.
Departamento de Zootecnia, Universidade Estadual de Maringá, Maringá, PR, Brasil.
E
E
Gasparino
A P
AP
Del Vesco
S E F
SE
Guimarães
S C C
SC
da Silva
S M
SM
Marcato
A R
AR
de Oliveira Neto
K R S
KR
Sousa
D000375:Aging / Q000235:genetics*; D000821:Animal Feed; D000818:Animals; D003370:Coturnix / Q000235:genetics* / Q000254:growth & development; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000254:growth & development / Q000378:metabolism*; D008928:Mitochondria / Q000235:genetics*; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D000071256:Uncoupling Protein 1; D015430:Weight Gain / Q000235:genetics
D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D000071256:Uncoupling Protein 1; D000226:Mitochondrial ADP, ATP Translocases; D003576:Electron Transport Complex IV