Fifteen Streptococcus suis serotypes identified by multiplex PCR.
A multiplex PCR was developed to detect 15 serotypes of Streptococcus suis. This multiplex PCR was separated into two reaction sets. The first set identified nine serotypes (serotypes 1/2, 1, 2, 3, 4, 7, 9, 14 and 16) and the second set identified six serotypes (serotypes 5, 8, 10, 19, 23 and 25). This assay correctly detected serotypes 2, 5 and 14 in human isolates, and serotypes 1, 2, 1/2, 3, 4, 5, 7, 9, 14, 16 and 19 in pig isolates from Thailand. No cross-reaction was observed with other streptococcal species. This assay may be useful for the serotype surveillance of human and pig isolates of S. suis.
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Anusak
A
Kerdsin
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Surang
S
Dejsirilert
Laboratory for Clinical Research on Infectious Diseases, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Yukihiro
Y
Akeda
Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Tsutomu
T
Sekizaki
Thailand-Japan Research Collaboration Center for Emerging and Re-emerging Infections, Nonthaburi, Thailand.
Shigeyuki
S
Hamada
Faculty of Veterinary Medicine, University of Montreal, Québec, Canada.
Marcelo
M
Gottschalk
Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjyuku, Tokyo, Japan.|Laboratory for Clinical Research on Infectious Diseases, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Kazunori
K
Oishi
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D012703:Serotyping; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000662:veterinary; D017011:Streptococcus suis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D004269:DNA, Bacterial