Evaluation of antibacterial, antioxidant and DNA protective capacity of Chenopodium album's ethanolic leaf extract.
We have investigated the antibacterial effects of Chenopodium album's ethanolic leaf extract (CAE) on all the Gram (+) and Gram (-) microorganisms and evaluated the protective effects of CAE on both yeast and human mononuclear leukocytes' genomic DNA upon oxidative shock. Antibacterial activity was recorded on Bacillus subtilis with 13 mm of inhibition zone. Total oxidative status (TOS) and the total antioxidative status (TAS) levels were determined to evaluate the antioxidant activity of CAE. Results indicated that there was a good correlation between dose of CAE and TAS levels. We also observed that CAE protect the DNA of both yeast and mononuclear leukocytes against the damaging effect of hydrogen peroxide. The comet assay, applied on both Saccharomyces cerevisiae BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) and human leukocytes, results suggested that there was statistically significant correlation between CAE dilutions and antigenotoxic activity.
Afyon Kocatepe University, Faculty of Science and Literatures, Biology Department, 03200 Afyonkarahisar, Turkey.
S
S
Elif Korcan
Onur
O
Aksoy
S Feyza
SF
Erdoğmuş
İ Hakkı
İH
Ciğerci
Muhsin
M
Konuk
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D027463:Chenopodium album / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects; D000431:Ethanol / Q000737:chemistry; D006801:Humans; D007962:Leukocytes; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D012441:Saccharomyces cerevisiae
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010936:Plant Extracts; D000431:Ethanol