The active ingredients of Jiang-Zhi-Ning: study of the Nelumbo nucifera alkaloids and their main bioactive metabolites.
The object of this study was to identify the major active ingredients of the Chinese Traditional Medicine Jiang-Zhi-Ning (JZN) based on the high performance liquid chromatography (HPLC) profiles of plasma samples obtained from beagle dogs at different times after intragastric administration of JZN, crude JZN extracts, different extracted fractions, different subfractions of the active fraction and different isolated ingredients. 2-Hydroxy-1-methoxyaporphin (2H1M), an alkaloid from Nelumbo nucifera, one of the herbs that make up JZN, was identified as the constituent showing the major pharmacodynamic effect. The major metabolites of 2H1M were analyzed and identified as N-demethyl-2-hydroxy-1-methoxyaporphine-2-O-glycuronic acid, 2-hydroxy-1-methoxy-aporphine-2-O-glycuronic acid and 2-hydroxy-1-methoxy-aporphine-2-O-sulphate. This study provided a comprehensive insight into the active components of JZN.
Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029, China.
Jianxin
J
Chen
Xueling
X
Ma
Kuo
K
Gao
Yong
Y
Wang
Huihui
H
Zhao
Hao
H
Wu
Juan
J
Wang
Hua
H
Xie
Yulin
Y
OuYang
Liangtao
L
Luo
Shuzhen
S
Guo
Jing
J
Han
Bing
B
Liu
Wei
W
Wang
D000470:Alkaloids / Q000737:chemistry* / Q000493:pharmacokinetics; D000818:Animals; D001060:Aporphines / Q000737:chemistry / Q000493:pharmacokinetics; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000493:pharmacokinetics; D008658:Inactivation, Metabolic; D031653:Nelumbo / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000493:pharmacokinetics
D000470:Alkaloids; D001060:Aporphines; D004365:Drugs, Chinese Herbal; C576907:Jiang-Zhi-Ning; D010936:Plant Extracts