Amperometric carbon paste enzyme electrodes with Fe(3)O(4) nanoparticles and 1,4-benzoquinone for glucose determination.
Two new amperometric carbon paste enzyme electrodes including Fe(3)O(4) nanoparticles with and without 1,4-benzoquinone were developed for glucose determination. Electron transfer properties of unmodified and Fe(3)O(4) nanoparticles and/or 1,4-benzoquinone modified carbon paste electrodes were investigated in 0.1 M KCl support electrolyte containing Fe(CN)6(3-/4-) as redox probe by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods. Fe(3)O(4) nanoparticles increased electron transfer at solution/electrode interface. The parameters affecting the analytical performance of the enzyme electrode have been investigated in detail and optimized for Fe(3)O(4) nanoparticle modified enzyme electrode (Fe(3)O(4)-CPEE). Fe(3)O(4) nanoparticles and 1,4-benzoquinone modified enzyme electrode (BQ-Fe(3)O(4)-CPEE) exhibited linear response from 1.9 × 10(-7) M to 3.7 × 10(-6) M, from 7.2 × 10(-6) M to 1.5 × 10(-4) M and from 1.3 × 10(-3) M to 1.2 × 10(-2) M with an excellent detection limit of 1.9 × 10(-8) M. BQ-Fe(3)O(4)-CPEE was used for determination of glucose in serum samples and results were in good agreement with those obtained by spectrophotometric method.
Faculty of Science, Department of Chemistry, Ankara University, Tandoğan, Ankara, Turkey. erdenpe@gmail.com
Pınar Esra
PE
Erden
Bülent
B
Zeybek
Şule
Ş
Pekyardımcı
Esma
E
Kılıç
D001234:Aspergillus niger / Q000737:chemistry / Q000201:enzymology; D016227:Benzoquinones / Q000737:chemistry*; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D002138:Calibration; D002244:Carbon / Q000737:chemistry*; D002384:Catalysis; D011232:Chemical Precipitation; D058266:Dielectric Spectroscopy; D004579:Electron Transport; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D014238:Trichloroacetic Acid
D016227:Benzoquinones; D001786:Blood Glucose; D005656:Fungal Proteins; C004532:quinone; D014238:Trichloroacetic Acid; D002244:Carbon; D005949:Glucose Oxidase; D052203:Ferrosoferric Oxide