Evidence for a role of GABA- and glutamate-gated chloride channels in olfactory memory.
In the honeybee, we investigated the role of transmissions mediated by GABA-gated chloride channels and glutamate-gated chloride channels (GluCls) of the mushroom bodies (MBs) on olfactory learning using a single-trial olfactory conditioning paradigm. The GABAergic antagonist picrotoxin (PTX) or the GluCl antagonist L-trans-pyrrolidine-2,4-dicarboxylic acid (L-trans-PDC) was injected alone or in combination into the α-lobes of MBs. PTX impaired early long-term olfactory memory when injected before conditioning or before testing. L-trans-PDC alone induced no significant effect on learning and memory but induced a less specific response to the conditioned odor. When injected before PTX, L-trans-PDC was able to modulate PTX effects. These results emphasize the role of MB GABA-gated chloride channels in consolidation processes and strongly support that GluCls are involved in the perception of the conditioned stimulus.
Centre de Recherches sur la Cognition Animale, Université Paul Sabatier Toulouse III, CNRS UMR 5169, 118 Route de Narbonne, 31062 Toulouse Cedex 4, France.
Katia
K
Boumghar
Thomas
T
Couret-Fauvel
Mikael
M
Garcia
Catherine
C
Armengaud
D000818:Animals; D001516:Bees / Q000502:physiology*; D018118:Chloride Channels / Q000378:metabolism*; D003213:Conditioning, Psychological; D003998:Dicarboxylic Acids / Q000008:administration & dosage / Q000494:pharmacology; D018756:GABA Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D018698:Glutamic Acid / Q000378:metabolism; D015640:Ion Channel Gating; D007858:Learning / Q000187:drug effects / Q000502:physiology; D008568:Memory / Q000187:drug effects / Q000502:physiology*; D024521:Mushroom Bodies / Q000187:drug effects / Q000294:innervation / Q000378:metabolism; D014179:Neurotransmitter Uptake Inhibitors / Q000008:administration & dosage / Q000494:pharmacology; D009812:Odorants; D010852:Picrotoxin / Q000008:administration & dosage / Q000494:pharmacology; D011759:Pyrrolidines / Q000008:administration & dosage / Q000494:pharmacology; D012903:Smell / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D018118:Chloride Channels; D003998:Dicarboxylic Acids; D018756:GABA Antagonists; D018698:Glutamic Acid; D014179:Neurotransmitter Uptake Inhibitors; D010852:Picrotoxin; D011759:Pyrrolidines; D005680:gamma-Aminobutyric Acid; C083486:pyrrolidine-2,4-dicarboxylic acid