Subchronic toxicity study of GH transgenic carp.
A subchronic toxicity study of GH (growth hormone) transgenic carp was carried out with 60 SD rats aged 4 weeks, weight 115∼125 g. Ten male and 10 female rats were allotted into each group. Animals of the three groups (transgenic carp group (GH-TC), parental carp group (PC) and control group) were fed soy- and alfalfa-free diet (SAFD) with 10% GH transgenic carp powder, 10% parental carp powder or 10% common carp powder for 90 consecutive days, respectively. In the end of study, animals were killed by exsanguination via the carotid artery under diethyl ether anesthesia, then weights of heart, liver, kidneys, spleen, thymus, brain, ovaries and uterus/testis were measured. Pathological examination of organs was determined. Endocrine hormones of triiodothyronine (T3), thyroid hormone (T4), follicle-stimulating hormone (FSH), 17β-estradiol (E2), progesterone (P) and testosterone (T) levels were detected by specific ELISA kit. Parameters of blood routine and blood biochemical were measured. The weights of the body and organs of the rats, food intake, blood routine, blood biochemical test and serum hormones showed no significant differences among the GH transgenic carp-treated, parental carp-treated and control groups (P>0.05). Thus, it was concluded that at the dose level of this study, GH transgenic carp showed no subchronic toxicity and endocrine disruption to SD rats.
National Institute for Nutrition and Food Safety, China CDC, Beijing 100021, China.
Ling
L
Yong
Yu-Mei
YM
Liu
Xu-Dong
XD
Jia
Ning
N
Li
Wen-Zhong
WZ
Zhang
D000818:Animals; D030801:Animals, Genetically Modified; D002347:Carps / Q000235:genetics*; D005260:Female; D005396:Fish Products / Q000633:toxicity*; D030841:Food, Genetically Modified / Q000633:toxicity*; D013006:Growth Hormone / Q000235:genetics*; D006321:Heart / Q000033:anatomy & histology / Q000187:drug effects; D006728:Hormones / Q000097:blood; D007668:Kidney / Q000033:anatomy & histology / Q000187:drug effects; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects; D008297:Male; D000455:Medicago sativa; D009929:Organ Size / Q000187:drug effects; D010053:Ovary / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013025:Glycine max; D013154:Spleen / Q000033:anatomy & histology / Q000187:drug effects; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects; D059550:Toxicity Tests, Subchronic / Q000379:methods
D006728:Hormones; D013006:Growth Hormone