Urinary proteome analysis in congenital bilateral hydronephrosis.
OBJECTIVE
A proteomics strategy was applied to map protein changes in urine after relief of congenital bilateral hydronephrosis to identify proteins correlated with the pathophysiological processes in congenital obstructive nephropathy as potential urinary biomarkers.


METHODS
Urine samples from 10 infants with bilateral abnormal drainage from the kidneys were collected at the time of relief from obstruction, and after 2 and 4 weeks. Proteomics techniques were used on samples from three patients for identification of protein changes between the three time-points, and enzyme-linked immunosorbent assay (ELISA) was used on samples from all 10 patients for validation of five selected proteins.


RESULTS
Mass spectrometry quantified 315 protein hits, out of which 33 proteins showed significantly changed urinary excretion between the time-points. Validation by ELISA showed high urinary excretion of fibrinogen, plasminogen, transthyretin and transferrin at the time of relief from obstruction, followed by a significant reduction. In contrast, Tamm-Horsfall protein exhibited the reverse pattern.


CONCLUSIONS
Using a mass spectrometry-based proteomics approach, this study identified 33 proteins related to congenital bilateral hydronephrosis, and pinpointed a panel of five proteins consistently linked to this congenital kidney disorder as potential urinary biomarkers.
The Water and Salt Research Center, Institute of Clinical Medicine, University of Aarhus, Aarhus, Denmark.
Lene
L
Stødkilde
Mia Gebauer
MG
Madsen
Johan
J
Palmfeldt
Sükrü Oguzkan
SO
Topcu
Rikke
R
Nørregaard
Lars Henning
LH
Olsen
Troels Munch
TM
Jørgensen
Jørgen
J
Frøkiær
D015415:Biomarkers / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005340:Fibrinogen / Q000652:urine*; D005500:Follow-Up Studies; D006801:Humans; D006869:Hydronephrosis / Q000151:congenital* / Q000601:surgery / Q000652:urine*; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D013058:Mass Spectrometry; D009403:Nephrostomy, Percutaneous; D010958:Plasminogen / Q000652:urine*; D011228:Prealbumin / Q000652:urine*; D011446:Prospective Studies; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015203:Reproducibility of Results; D014168:Transferrin / Q000652:urine*; D058949:Uromodulin / Q000652:urine*
D015415:Biomarkers; D011228:Prealbumin; D020543:Proteome; D014168:Transferrin; D058949:Uromodulin; D005340:Fibrinogen; D010958:Plasminogen