Expression of connexin 26 and bovine papillomavirus E5 in cutaneous fibropapillomas of cattle.
Bovine papillomaviruses (BPVs) can infect epithelial cells and fibroblasts, inducing fibropapillomas in cattle. Gap junctions are communication channels between cells composed of connexins (Cxs). This study evaluated expression of Cx26 and the major BPV oncoprotein E5 in bovine cutaneous fibropapillomas. BPV DNA was amplified from 20/20 fibropapillomas and 3/3 samples of normal skin. All fibropapillomas (20/20) were positive by immunostaining for E5, whereas the three normal skin samples were negative. Cx26 was expressed faintly in the normal skin epithelium. Positive cytoplasmic and juxtanuclear immunoreactivity for Cx26 was evident in 18/20 (90%) fibropapillomas. Western blot analysis demonstrated higher expression of Cx26 in 6/6 fibropapillomas compared to normal skin samples.
Department of Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rêgo, 1235, Cidade Universitária, 50740521 Recife, Pernambuco, Brazil.
Maria Angelica
MA
Silva
Gennaro
G
Altamura
Annunziata
A
Corteggio
Franco
F
Roperto
Florentina
F
Bocaneti
Elena
E
Velescu
Antonio C
AC
Freitas
Cybelle C R
CC
Carvalho
Karen P S
KP
Cavalcanti
Giuseppe
G
Borzacchiello
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000821:virology; D000072259:Connexin 26; D017630:Connexins / Q000378:metabolism*; D052687:Deltapapillomavirus / Q000145:classification*; D005260:Female; D005786:Gene Expression Regulation; D030361:Papillomavirus Infections / Q000662:veterinary*; D016133:Polymerase Chain Reaction
D017630:Connexins; D000072259:Connexin 26