Assessing pre-implantation embryo development in mice provides a rationale for understanding potential adverse effects of ART and PGD procedures.
Although the molecular events controlling human pre-implantation development remain unclear, mechanisms have been identified by analyzing these stages in mice. Through this approach, considerable insight has been gained into the events that operate to determine the first two cell fate decisions, occurring from zygote formation to the blastocyst prior to implantation. These mechanisms are related to cell polarization, cell division, cell-cell contact, and cell spatial position. Two developmental stages are essential for these processes to proceed adequately. Firstly, the second polar body must anchor to the external membrane during the first mitotic divisions of the embryo as its position is strongly biased to determine the plane of polarity. This in turn has important influence on the fate of the early blastomeres. Secondly, in the transition from the 8- to 16-cell stage, the cells that will form the inner cell mass are determined. Moreover, analyses performed on human oocytes and embryos have identified similar processes to those reported in mice and thus are evolutionarily conserved. Therefore, the understanding of mice pre-implantation embryo development provides a rationale to interpret current results of potential long-term adverse outcomes of Assisted Reproductive Technologies and Pre-implantation Genetic Diagnosis (PGD).
Facultad de Medicine, Departmento de Farmacología, Universidad Complutense de Madrid, Spain. mlmartinez.frias@isciii.es
María Luisa
ML
Martínez-Frías
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D016764:Cell Polarity; D004622:Embryo, Mammalian / Q000166:cytology*; D047108:Embryonic Development; D005260:Female; D005820:Genetic Testing; D006801:Humans; D051379:Mice; D059705:Polar Bodies