Inherent variation in stable isotope values and discrimination factors in two life stages of green turtles.
We examine inherent variation in carbon and nitrogen stable isotope values of multiple soft tissues from a population of captive green turtles Chelonia mydas to determine the extent of isotopic variation due to individual differences in physiology. We compare the measured inherent variation in the captive population with the isotopic variation observed in a wild population of juvenile green turtles. Additionally, we measure diet-tissue discrimination factors to determine the offset that occurs between isotope values of the food source and four green turtle tissues. Tissue samples (epidermis, dermis, serum, and red blood cells) were collected from captive green turtles in two life stages (40 large juveniles and 30 adults) at the Cayman Turtle Farm, Grand Cayman, and analyzed for carbon and nitrogen stable isotopes. Multivariate normal models were fit to the isotope data, and the Bayesian Information Criterion was used for model selection. Inherent variation and discrimination factors differed among tissues and life stages. Inherent variation was found to make up a small portion of the isotopic variation measured in a wild population. Discrimination factors not only are tissue and life stage dependent but also appear to vary with diet and sea turtle species, thus highlighting the need for appropriate discrimination factors in dietary reconstructions and trophic-level estimations. Our measures of inherent variation will also be informative in field studies employing stable isotope analysis so that differences in diet or habitat are more accurately identified.
Archie Carr Center for Sea Turtle Research and Department of Biology, University of Florida, P.O. Box 118525, Gainesville, FL 32611, USA. hvz@ufl.edu
Hannah B
HB
Vander Zanden
Karen A
KA
Bjorndal
Walter
W
Mustin
José Miguel
JM
Ponciano
Alan B
AB
Bolten
D000818:Animals; D001444:Bahamas; D001499:Bayes Theorem; D001823:Body Composition; D002244:Carbon / Q000032:analysis / Q000097:blood; D002247:Carbon Isotopes / Q000032:analysis / Q000097:blood; D004032:Diet; D004817:Epidermis / Q000737:chemistry; D005260:Female; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry; D015999:Multivariate Analysis; D009584:Nitrogen / Q000032:analysis / Q000097:blood; D009587:Nitrogen Isotopes / Q000032:analysis / Q000097:blood; D018709:Statistics, Nonparametric; D014018:Tissue Distribution; D014426:Turtles / Q000254:growth & development* / Q000378:metabolism*; D014900:West Indies
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D002244:Carbon; D009584:Nitrogen