Light therapy: complementary antibacterial treatment of oral biofilm.
Conventional antibacterial treatment fails to eradicate biofilms associated with common infections of the oral cavity. Unlike chemical agents, which are less effective than anticipated, owing to diffusion limitations in biofilms, light is more effective on bacteria in biofilm than in suspension. Effectiveness depends also on the type and parameters of the light. We tested the phototoxic effects of non-coherent blue light (wavelengths, 400-500 nm) and CO(2) laser (wavelength, 10.6 μm), which have different mechanisms of action on the oral bacterium Streptoccocus mutans, in biofilm and on tooth enamel. Exposure of S. mutans in biofilm to blue light had a delayed effect on bacterial viability throughout the biofilm and a sustained antibacterial effect on biofilm newly formed by previously irradiated bacteria. A synergistic antibacterial effect between blue light and H(2)O(2) may enhance the phototoxic effect, which involves a photochemical mechanism mediated by reactive oxygen species (ROS) formation. The effect of CO(2) laser irradiation on the viability of S. mutans in biofilm on enamel samples appeared to be higher in the deep layers, due to heating of the enamel surface by the absorbed energy. Biofilms do not interfere with the chemical changes resulting from irradiation, which may increase the enamel's resistance to acid attack.
Department of Prosthodontics, The Hebrew University-Hadassah School of Dental Medicine, P.O.B. 12272, Jerusalem 91120, Israel. osnatf@ekmd.huji.ac.il
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D018441:Biofilms / Q000528:radiation effects*; D003743:Dental Enamel / Q000528:radiation effects*; D003773:Dental Plaque / Q000532:radiotherapy*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D054020:Lasers, Gas / Q000009:adverse effects / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D050296:Microbial Viability / Q000528:radiation effects; D010789:Phototherapy / Q000379:methods*; D013295:Streptococcus mutans / Q000528:radiation effects*
D006861:Hydrogen Peroxide