Acinetobacter species isolates from a range of environments: species survey and observations of antimicrobial resistance.
Acinetobacter species isolates from a range of environments, including soil, were investigated. We determined 16S rRNA and rpoB gene sequences for species identification and performed tests of antimicrobial resistance susceptibility. Twenty-nine of the isolates (8 from soil and 21 from life environment) belonged to the genus Acinetobacter. Fourteen Acinetobacter species were identified among 29 isolates: 4 A. baumannii, 3 A. calcoaceticus, 1 A. nosocomialis, 2 A. pittii, and 2 Acinetobacter gen. sp. 'close to 13TU' as A. calcoaceticus-baumannii complex. Three Acinetobacter species isolates were identified as novel species candidates. Three Acinetobacter species isolates were resistant to imipenem: 1 A. parvus and 2 novel species candidates of Acinetobacter. Eight isolates showed resistance to colistin: all Acinetobacter gen. sp. 'close to 13TU' (2 isolates) and A. parvus isolates (3 isolates) were resistant to colistin. Although the genotypes of A. baumannii isolates from various natural environments were different from those of clinical isolates, the presence of clinically important and antimicrobial resistant Acinetobacter species in the natural environment may represent a threat to public health.
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, South Korea.
Ji-Young
JY
Choi
Yejin
Y
Kim
Eun Ah
EA
Ko
Young Kyoung
YK
Park
Weon-Hwa
WH
Jheong
GwangPyo
G
Ko
Kwan Soo
KS
Ko
D000150:Acinetobacter / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004783:Environmental Microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit