Deep sequencing reveals complex spurious transcription from transiently transfected plasmids.
Transient plasmid transfection is a common approach in studies in cultured mammalian cells. To examine behavior of transfected plasmids, we analyzed their transcriptional landscape by deep sequencing. We have found that the entire plasmid sequence is transcribed at different levels. Spurious transcription may have undesirable effects as some plasmids, when co-transfected, inhibited expression of luciferase reporters in a dose-dependent manner. In one case, we attributed this effect to a Kan/Neo resistance cassette, which generated a unique population of edited sense and antisense small RNAs. The unexpected complexity of expression from transiently transfected plasmids underscores the importance of appropriate experimental controls.
Institute of Molecular Genetics AS CR, Prague, Czech Republic.
Jana
J
Nejepinska
Radek
R
Malik
Martin
M
Moravec
Petr
P
Svoboda
D002460:Cell Line; D005434:Flow Cytometry; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D016372:RNA, Antisense / Q000235:genetics
D016372:RNA, Antisense