Disease resistance to Pectobacterium carotovorum is negatively modulated by the Arabidopsis Lectin Receptor Kinase LecRK-V.5.
Plant stomata function in disease resistance by restricting bacteria entry inside leaves. During plant-bacteria interactions, stomatal closure is initiated by the recognition of Microbe-Associated Molecular Patterns (MAMPs). Recently, we have shown that the Lectin Receptor Kinase V.5 (LecRK-V.5) negatively regulates bacterium- and MAMP-induced stomatal closure upstream of Reactive Oxygen Species (ROS) production mediated by abscisic acid signaling. Closed stomata in lecrk-V.5 mutants are correlated with constitutive high level of ROS in guard cells. Consequently, lecrk-V.5 mutants are more resistant to hemi-biotrophic pathogen Pseudomonas syringae pv tomato DC3000 (Pst DC3000). In this report, we further investigate the role of LecRK-V.5 in resistance against necrotrophic bacteria Pectobacterium carotovorum ssp. carotovorum (Pcc). Upon surface-inoculation lecrk-V.5 mutants exhibited enhanced resistance against Pcc whereas a wild-type level of resistance was observed using infiltration-inoculation, an inoculation method that bypasses the epidermal barrier. Enhanced resistance of dip-inoculated lecrk-V.5 mutants against necrotrophic bacteria, that induce different defense responses than hemi-biotrophic bacteria, further suggests a possible role for LecRK-V.5 in stomatal immunity.
Department of Life Science and Institute of Plant Biology; National Taiwan University; Taipei, Taiwan.
Dominique
D
Arnaud
Marie
M
Desclos-Theveniau
Laurent
L
Zimmerli
D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D060467:Disease Resistance / Q000235:genetics* / Q000502:physiology; D017343:Genes, Plant; D009154:Mutation; D016973:Pectobacterium carotovorum; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D019441:Plant Epidermis / Q000502:physiology; D018515:Plant Leaves / Q000502:physiology; D054046:Plant Stomata / Q000502:physiology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
D029681:Arabidopsis Proteins; D017382:Reactive Oxygen Species; D000040:Abscisic Acid; C099543:ATHLECRK protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases