Controlled enzyme-catalyzed degradation of polymeric capsules templated on CaCO₃: influence of the number of LbL layers, conditions of degradation, and disassembly of multicompartments.
Enzyme-catalyzed degradation of CaCO₃-templated capsules is presented. We investigate a) biodegradable, b) mixed biodegradable/synthetic, and c) multicompartment polyelectrolyte multilayer capsules with different numbers of polymer layers. Using confocal laser scanning microscopy we observed the kinetics of the non-specific protease Pronase-induced degradation of capsules is slowed down on the order of hours by either increasing the number of layers in the wall of biodegradable capsules, or by inserting synthetic polyelectrolyte multilayers into the shell comprised of biodegradable polymers. The degradation rate increases with the concentration of Pronase. Controlled detachment of subcompartments of multicompartment capsules, with potential for intracellular delivery or in-vivo applications, is also shown.
Institute of Crystallography, Russian Academy of Sciences, Moscow 119333, Russia.
Irina
I
Marchenko
Alexey
A
Yashchenok
Tatiana
T
Borodina
Tatiana
T
Bukreeva
Manfred
M
Konrad
Helmuth
H
Möhwald
Andre
A
Skirtach
D002119:Calcium Carbonate / Q000737:chemistry*; D002214:Capsules / Q000737:chemistry*; D002384:Catalysis; D003911:Dextrans / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D011099:Polyglutamic Acid / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011402:Pronase / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013451:Sulfonic Acids / Q000737:chemistry
D002214:Capsules; D003911:Dextrans; D005456:Fluorescent Dyes; D010455:Peptides; D011073:Polyamines; D011108:Polymers; D012235:Rhodamines; D013451:Sulfonic Acids; C015219:fluorescein isothiocyanate dextran; C015462:polyarginine; D011099:Polyglutamic Acid; C017645:polyaspartate; C063994:polyallylamine; C009434:tetramethylrhodamine isothiocyanate; D012822:Silicon Dioxide; D011402:Pronase; D002119:Calcium Carbonate; D016650:Fluorescein-5-isothiocyanate; C077114:styrenesulfonic acid polymer