Arsenate and arsenite exposure modulate antioxidants and amino acids in contrasting arsenic accumulating rice (Oryza sativa L.) genotypes.
Carcinogenic arsenic (As) concentrations are found in rice due to irrigation with contaminated groundwater in South-East Asia. The present study evaluates comparative antioxidant property and specific amino acid accumulation in contrasting rice genotypes corresponding to differential As accumulation during arsenate (As(V)) and arsenite (As(III)) exposures. The study was conducted on two contrasting As accumulating rice genotypes selected from 303 genotype accessions, in hydroponic conditions. Maximum As accumulation was up to 1181 μg g(-1) dw in the roots of high As accumulating genotype (HARG), and 89 μg g(-1) dw in low As accumulating genotype (LARG) under As(III) exposures. The inorganic As was correlated more significantly upon exposures to As(III) than As(V). In the presence of As(V) various antioxidant enzymes guiacol peroxidase (GPX), ascorbate peroxidase (APX) and superoxide dismutase (SOD) were highly stimulated in HARG. The stress responsive amino acids proline, cysteine, glycine, glutamic acid and methionine showed higher accumulation in HARG than LARG. A clear correlation was found between stress responsive amino acids, As accumulation and antioxidative response. The comparisons between the contrasting genotypes helped to determine the significance of antioxidants and specific amino acid response to As stress.
Council of Scientific and Industrial Research - National Botanical Research Institute (CSIR-NBRI), Lucknow 226001, India; Amity Institute of Environmental Sciences, Amity University, Noida, India.
Richa
R
Dave
Rudra Deo
RD
Tripathi
Sanjay
S
Dwivedi
Preeti
P
Tripathi
Garima
G
Dixit
Yogesh Kumar
YK
Sharma
Prabodh Kumar
PK
Trivedi
Francisco J
FJ
Corpas
Juan B
JB
Barroso
Debasis
D
Chakrabarty
D000596:Amino Acids / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D001149:Arsenates / Q000737:chemistry*; D001151:Arsenic / Q000032:analysis / Q000737:chemistry; D018053:Arsenites / Q000737:chemistry*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D005838:Genotype; D060587:Groundwater; D018527:Hydroponics; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000737:chemistry; D008670:Metals / Q000737:chemistry; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000737:chemistry; D012989:Soil Pollutants / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism
D000596:Amino Acids; D000975:Antioxidants; D001149:Arsenates; D018053:Arsenites; D008670:Metals; D017382:Reactive Oxygen Species; D012989:Soil Pollutants; D008315:Malondialdehyde; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D009195:Peroxidase; D013482:Superoxide Dismutase; C015001:arsenite; D001151:Arsenic; C025657:arsenic acid