River waters induced neurotoxicity in an embryo-larval zebrafish model.
Some investigations have revealed an increased release of psychoactive drugs into the aquatic environments near big cities. However, despite the alert generated by the presence of such neurotoxic compounds, there is a lack of studies evaluating the impact on living organisms. One solution consists in the development of bioassays able to address specific risks, such as neurotoxicity, but on the other hand suitable to assess complex matrices like river samples. The objective of this work was to assess surface water toxicity by means of a zebrafish embryo-larval combined toxicity assay, which is based on a variety of toxicological endpoints, especially those related to neurodevelopment. For such a purpose, we selected the Tagus River in which a previous monitoring study revealed the presence of psychoactive drugs. Results showed that most of the toxicological endpoints evaluated remained unaltered in the exposed embryos, except for the tail length that was larger in the exposed larvae, and the locomotor activity in the 6-day larvae, which was decreased in four groups of exposure (n=5 sampling points). In the absence of systemic toxicity, changes in larval locomotion are indicative of neurotoxicity. This result suggests that the Tagus River can convey neurotoxic compounds at levels that may represent an early and specific threat over the aquatic species of vertebrates, what can have dramatic consequences under the ecological point of view.
Toxicology Area, National Centre for Environmental Health, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain. jesuspgc@isciii.es
Jesús Pablo
JP
García-Cambero
Myriam
M
Catalá
Yolanda
Y
Valcárcel
D000818:Animals; D001681:Biological Assay; D004625:Embryo, Nonmammalian / Q000187:drug effects; D007814:Larva / Q000187:drug effects; D008124:Locomotion / Q000187:drug effects; D023421:Models, Animal; D009474:Neurons / Q000187:drug effects*; D011619:Psychotropic Drugs / Q000633:toxicity*; D045483:Rivers / Q000737:chemistry; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000196:embryology / Q000254:growth & development / Q000502:physiology*
D011619:Psychotropic Drugs; D014874:Water Pollutants, Chemical