Methyl-beta-cyclodextrin enhanced biodegradation of polycyclic aromatic hydrocarbons and associated microbial activity in contaminated soil.
The contamination of soils by polycyclic aromatic hydrocarbons (PAHs) is a widespread environmental problem and the remediation of PAHs from these areas has been a major concern. The effectiveness of many in situ bioremediation systems may be constrained by low contaminant bioavailability due to limited aqueous solubility or a large magnitude of sorption. The objective of this research was to evaluate the effect of methyl-beta-cyclodextrin (MCD) on bioaugmentation by Paracoccus sp. strain HPD-2 of an aged PAH-contaminated soil. When 10% (W/W) MCD amendment was combined with bioaugmentation by the PAH-degrading bacterium Paracoccus sp. strain HPD-2, the percentage degradation of total PAHs was significantly enhanced up to 34.8%. Higher counts of culturable PAH-degrading bacteria and higher soil dehydrogenase and soil polyphenol oxidase activities were observed in 10% (W/W) MCD-assisted bioaugmentation soil. This MCD-assisted bioaugmentation strategy showed significant increases (p < 0.05) in the average well color development (AWCD) obtained by the BIOLOG Eco plate assay, Shannon-Weaver index (H) and Simpson index (lambda) compared with the controls, implying that this strategy at least partially restored the microbiological functioning of the PAH-contaminated soil. The results suggest that MCD-aided bioaugmentation by Paracoccus sp. strain HPD-2 may be a promising practical bioremediation strategy for aged PAH-contaminated soils.
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. mmsun@issas.ac.cn
Mingming
M
Sun
Yongming
Y
Luo
Peter
P
Christie
Zhongjun
Z
Jia
Zhengao
Z
Li
Ying
Y
Teng
D001673:Biodegradation, Environmental / Q000187:drug effects; D002244:Carbon / Q000494:pharmacology; D004156:Catechol Oxidase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010230:Paracoccus / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity*; D047392:beta-Cyclodextrins / Q000494:pharmacology*
D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D002244:Carbon; D010088:Oxidoreductases; D004156:Catechol Oxidase