Gold nanoparticles-based nanoconjugates for enhanced enzyme cascade and glucose sensing.
We have coupled gold nanoparticles with horseradish peroxidase (HRP) to assemble catalytic nanoconjugates (HRP-AuNPs) for glucose detection. We found that a proper mixing ratio of HRP/AuNPs can significantly improve catalytic activity for the cascade reaction, an effect arising from increased spatial coupling between enzymes. Such gold nanoparticle-based nanoconjugates are shown to be promising nanosensors for glucose.
Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou 215123, China.
Dongdong
D
Zeng
Weijie
W
Luo
Jiang
J
Li
Huajie
H
Liu
Hongwei
H
Ma
Qing
Q
Huang
Chunhai
C
Fan
D003124:Colorimetry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*
D004800:Enzymes, Immobilized; D006046:Gold; D006735:Horseradish Peroxidase; D005947:Glucose