Antioxidant sol-gel improves cutaneous wound healing in streptozotocin-induced diabetic rats.
We examined the effects of vitamin C in Pluronic F127 on diabetic wound healing. Full-thickness excision skin wounds were made in normal and diabetic Wistar rats to evaluate the effect of saline, saline plus vitamin C (antioxidant sol), Pluronic F127, or Pluronic F127 plus vitamin C (antioxidant sol-gel). The rate of wound contraction, the levels of epidermal and dermal maturation, collagen synthesis, and apoptosis production in the wound tissue were determined. In vitro data showed that after 6 hours of air exposure, the order of the scavenging abilities for HOCl, H(2)O(2), and O(2) (-) was antioxidant sol-gel > antioxidant saline > Pluronic F127 = saline. After 7 and 14 days of wound injury, the antioxidant sol-gel improved wound healing significantly by accelerated epidermal and dermal maturation, an increase in collagen content, and a decrease in apoptosis formation. However, the wounds of all treatments healed mostly at 3 weeks. Vitamin C in Pluronic F127 hastened cutaneous wound healing by its antioxidant and antiapoptotic mechanisms through a good drug delivery system. This study showed that Pluronic F127 plus vitamin C could potentially be employed as a novel wound-healing enhancer.
Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
Yen-Hsien
YH
Lee
Jung-Jhih
JJ
Chang
Chiang-Ting
CT
Chien
Ming-Chien
MC
Yang
Hsiung-Fei
HF
Chien
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis; D001205:Ascorbic Acid / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism* / Q000503:physiopathology*; D016503:Drug Delivery Systems; D005260:Female; D006909:Hydroxyproline / Q000737:chemistry; D007150:Immunohistochemistry / Q000379:methods; D044367:Phase Transition; D020442:Poloxamer / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000473:pathology; D013311:Streptozocin / Q000494:pharmacology*; D013997:Time Factors; D014945:Wound Healing
D000975:Antioxidants; D017382:Reactive Oxygen Species; D020442:Poloxamer; D013311:Streptozocin; D001205:Ascorbic Acid; D006909:Hydroxyproline