[Study in the killing effect of Myxoma virus to C6 glioma cell in vitro].
OBJECTIVE
To evaluate the susceptibility of C6 glioma cells to Myxoma virus and the killing effect of Myxoma virus to the C6 glioma cells in vitro.


METHODS
C6 glioma cells were infected with myxoma virus, used death virus as the negative control, 5-FU as the positive control, DEMD as blank control. The number of living cells were counted every 24 h, and Western-Blot method, inverted microscope and MTT assay were applicated to observe the cell morphology and survival rate in each group.


RESULTS
The cell number were decreased rapidly in virus effected group and 5-FU group, with significant differences to the negative and blank control groups. And cells in virus effected group appeared cytopathic effect.


CONCLUSIONS
C6 glioma cells were susceptible to myxoma virus and myxoma virus had killing effect to C6 glioma cells in vitro.
Department of neurosurgery, Shenzhen 2nd hospital, Shenzhen 518035, China.
Meng
M
Zang
Qiu-Sheng
QS
Zhang
Shi-Jie
SJ
Liang
Tao
T
Ji
Heng-Zhou
HZ
Lin
Wei-Ping
WP
Li
D045744:Cell Line, Tumor; D005910:Glioma / Q000628:therapy*; D006801:Humans; D009233:Myxoma virus; D050130:Oncolytic Virotherapy; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology
D051057:Proto-Oncogene Proteins c-akt