G protein-coupled receptors participate in cytokinesis.
Cytokinesis, the last step during cell division, is a highly coordinated process that involves the relay of signals from both the outside and inside of the cell. We have a basic understanding of how cells regulate internal events, but how cells respond to extracellular cues is less explored. In a systematic RNAi screen of G protein-coupled receptors (GPCRs) and their effectors, we found that some GPCRs are involved in cytokinesis. RNAi knockdown of these GPCRs caused increased binucleated cell formation, and live cell imaging showed that most formed midbodies but failed at the abscission stage. OR2A4 (olfactory receptor, family 2, subfamily A, member 4) localized to cytokinetic structures in cells and its knockdown caused cytokinesis failure at an earlier stage, likely due to effects on the actin cytoskeleton. Identifying the downstream components that transmit GPCR signals during cytokinesis will be the next step and we show that GIPC1 (GIPC PDZ domain containing family, member 1), an adaptor protein for GPCRs, may play a part. RNAi knockdown of GIPC1 significantly increased binucleated cell formation. Understanding the molecular details of GPCRs and their interaction proteins in cytokinesis regulation will give us important clues about GPCRs signaling as well as how cells communicate with their environment during division.
Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA.
Xin
X
Zhang
Anne V
AV
Bedigian
Wenchao
W
Wang
Ulrike S
US
Eggert
D008841:Actin Cytoskeleton / Q000378:metabolism; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D048749:Cytokinesis; D055785:Gene Knockdown Techniques; D015726:Giant Cells / Q000166:cytology / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D034741:RNA, Small Interfering / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; C492009:GIPC1 protein, human; D034741:RNA, Small Interfering; D043562:Receptors, G-Protein-Coupled