Micronutrient status and global DNA methylation in school-age children.
Aberrations in global LINE-1 DNA methylation have been related to risk of cancer and cardiovascular disease. Micronutrients including methyl-donors and retinoids are involved in DNA methylation pathways. We investigated associations of micronutrient status and LINE-1 methylation in a cross-sectional study of school-age children from Bogotá, Colombia. Methylation of LINE-1 repetitive elements was quantified in 568 children 5-12 years of age using pyrosequencing technology. We examined the association of LINE-1 methylation with erythrocyte folate, plasma vitamin B12, vitamin A ferritin (an indicator of iron status) and serum zinc concentrations using multivariable linear regression. We also considered associations of LINE-1 methylation with socio-demographic and anthropometric characteristics. Mean (± SD) LINE-1 methylation was 80.25 (± 0.65) percentage of 5-mC (%5-mC). LINE-1 methylation was inversely related to plasma vitamin A. After adjustment for potential confounders, children with retinol levels higher than or equal to 1.05 µmol/L showed 0.19% 5-mC lower LINE-1 methylation than children with retinol levels lower than 0.70 µmol/L. LINE-1 methylation was also inversely associated with C-reactive protein, a marker of chronic inflammation, and female sex. We identified positive associations of maternal body mass index and socioeconomic status with LINE-1 methylation. These associations were not significantly different by sex. Whether modification of these exposures during school-age years leads to changes in global DNA methylation warrants further investigation.
Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MI, USA. perngwei@umich.edu
Wei
W
Perng
Laura S
LS
Rozek
Mercedes
M
Mora-Plazas
Ofra
O
Duchin
Constanza
C
Marin
Yibby
Y
Forero
Ana
A
Baylin
Eduardo
E
Villamor
D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D019175:DNA Methylation; D005260:Female; D005293:Ferritins / Q000097:blood; D005492:Folic Acid / Q000097:blood; D056726:Genetic Association Studies; D006801:Humans; D007501:Iron / Q000097:blood; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics*; D008297:Male; D018977:Micronutrients / Q000097:blood*; D012737:Sex Factors; D014801:Vitamin A / Q000097:blood; D014805:Vitamin B 12 / Q000097:blood; D015032:Zinc / Q000097:blood
D018977:Micronutrients; D014801:Vitamin A; D002097:C-Reactive Protein; D005293:Ferritins; D005492:Folic Acid; D007501:Iron; D015032:Zinc; D014805:Vitamin B 12