A theoretical study of the stability of DNA binding with cis/trans platin.
Both cis- and trans-platins are known to form intra- and interstrand cross-linking with DNA. Since the nature and strength of binding is different, it makes their efficacy as anti-tumour drug different. In the present communication, we report theoretical analysis by using an amended Zimm and Bragg theory, to explain the melting behaviour and heat capacity of DNA with and without platin binding. The sharpness of transition has been examined in terms of half width and sensitivity parameter (deltaH/sigma). The experimental measurements of Pilch et al (J Mol Biol 2000, 296, 803) and Ctirad and Brabec (J Biol Chem 2001, 276, 9655) have been used.
Physics Department, Integral University, Kursi Road, Lucknow 226 026, India.
Seema
S
Srivastava
Irfan Ali
IA
Khan
Shinoo
S
Srivastava
Vishwambhar Dayal
VD
Gupta
D000465:Algorithms; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D001483:Base Sequence; D002945:Cisplatin / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D006358:Hot Temperature; D006801:Humans; D008956:Models, Chemical; D008962:Models, Theoretical; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D013696:Temperature
D000970:Antineoplastic Agents; D003432:Cross-Linking Reagents; C034697:transplatin; D004247:DNA; D002945:Cisplatin