Expression of Eps8 correlates with poor survival in oral squamous cell carcinoma.
OBJECTIVE
Epidermal growth factor receptor pathway substrate 8 (Eps8) is a signaling protein implicated in the development of many human cancers including oral squamous cell carcinoma (OSCC). This study examined the expression of Eps8 and assessed its significance in patients with OSCC.


METHODS
Immunohistochemical staining for Eps8 was conducted in 205 cases of OSCC collected over 7 years. The results were analyzed and correlated with patients' clinical outcomes.


RESULTS
We identified Eps8 expression in 186 of the 205 cases of OSCC (91%) and the aberrance occurred primarily in the cytoplasm of OSCC cells. Univariate analysis revealed that patients with Eps8 expression had significantly poorer 5-year overall survival (OS) than those without it (43% vs 74%, P = 0.014). Eps8 expression was also identified as an independent predictive factor for poorer OS by a multivariate analysis in regression modeling (P = 0.021, HR = 2.7). A Kaplan-Meier analysis showed that patients with positive Eps8 expression had a significantly poorer OS than patients with negative/low Eps8 expression (P = 0.038). The difference in disease-free survival between positive Eps8 expression and negative/low Eps8 expression nearly reached statistical significance (P = 0.051).


CONCLUSIONS
Eps8 is frequently expressed in OSCC. The aberrant expression of Eps8 closely correlated with poor survival in patients with OSCC.
Department of Pathology, St Martin De Porres Hospital, Chiayi.
Pei-Yi
PY
Chu
Jia-Hung
JH
Liou
Yueh-Min
YM
Lin
Chih-Jung
CJ
Chen
Mu-Kuan
MK
Chen
Shu-Hui
SH
Lin
Chung-Min
CM
Yeh
Hsin-Kai
HK
Wang
Ming-Chei
MC
Maa
Tzeng-Horng
TH
Leu
Nai-Wen
NW
Chang
Nicholas C
NC
Hsu
Kun-Tu
KT
Yeh
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000235:genetics; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism*; D016612:Paraffin Embedding; D011379:Prognosis; D012189:Retrospective Studies; D016019:Survival Analysis
D048868:Adaptor Proteins, Signal Transducing; C489989:EPS8 protein, human