Biodentine induces immortalized murine pulp cell differentiation into odontoblast-like cells and stimulates biomineralization.
BACKGROUND
Biodentine (Septodont, Saint Maur des Faussés, France), a new tricalcium silicate-based cement, has recently been commercialized and advertised as a bioactive material. Its clinical application and physical properties have been widely described, but, so far, its bioactivity and biological effect on pulp cells have not been clearly shown. Thus, the aim of this study was to evaluate the biological effect of Biodentine on immortalized murine pulp cells (OD-21).


METHODS
OD-21 cells were cultured with or without Biodentine. Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) colorimetric assay after 2, 3, and 5 days of stimulation. The expression of several biomolecular markers was analyzed to screen differentiation pathways, both on a gene level with Real-time reverse transcription polymerase chain reaction and on a protein level by measuring alkaline phosphatase activity. Alizarin red staining was used to assess and quantify biomineralization.


RESULTS
The expression patterns of several genes confirmed the differentiation of OD-21 cells into odontoblasts during the period of cell culture. Our results suggest that Biodentine is bioactive because it increased OD-21 cell proliferation and biomineralization in comparison with controls.


CONCLUSIONS
Because of its bioactivity, Biodentine can be considered as a suitable material for clinical indications of dentin-pulp complex regeneration, such as direct pulp capping.
Centre de Recherche des Cordeliers, INSERM UMRS872, Paris, France.
Marjorie
M
Zanini
Jean Michel
JM
Sautier
Ariane
A
Berdal
Stéphane
S
Simon
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000880:Anthraquinones; D001672:Biocompatible Materials / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis; D002113:Calcification, Physiologic / Q000187:drug effects; D017610:Calcium Compounds / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000032:analysis; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D018398:Homeodomain Proteins / Q000032:analysis; D008422:Materials Testing; D051379:Mice; D015675:Osteocalcin / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D017640:Silicates / Q000494:pharmacology*; D000076182:Sp7 Transcription Factor; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D014157:Transcription Factors / Q000032:analysis; D016335:Zinc Fingers / Q000187:drug effects
D000880:Anthraquinones; D001672:Biocompatible Materials; D015415:Biomarkers; D017610:Calcium Compounds; D024042:Collagen Type I; D004396:Coloring Agents; D050659:Core Binding Factor Alpha 1 Subunit; C501120:Dlx5 protein, mouse; D018398:Homeodomain Proteins; C084119:MSX2 protein; C493716:Runx2 protein, mouse; D017640:Silicates; D000076182:Sp7 Transcription Factor; C479466:Sp7 protein, mouse; D013778:Tetrazolium Salts; D013844:Thiazoles; D014157:Transcription Factors; D015675:Osteocalcin; C070380:3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium; C004468:Alizarin Red S; C506393:tricalcium silicate; D000469:Alkaline Phosphatase