Rapid reversed-phase liquid chromatography separation of cyclolinopeptides with monolithic and microparticulate columns.
Three monolithic C(18)-bonded silica gel columns i.e. Chromolith SpeedROD (CSR), Chromolith Performance (CP), and Chromolith High Resolution (CHR), MerckKGaA Darmstadt, Germany and two particle-based columns i.e. ZORBAX Eclipse XDB-C(18) (ZEX), Agilent and POROS R1/20 (POR), Applied Biosystems were compared for their performance in separating a mixture of flaxseed cyclolinopeptides (CLs). Gradient mobile phases of acetonitrile and water were optimized for each column. The performance of CHR column in profiling CL standards, measured as the resolution of individual CL, selectivity, and peak asymmetry exceeded the performance of traditional particle-packed columns and the other monolithic columns. The profiling of CLs in aqueous methanolic flaxseed extract was optimized for high-throughput analysis. A total analysis time of 1.5 min at a flow rate of 3.0mLmin(-1) was achieved on a CSR column. Injection of over 2000 methanol extracts of flaxseed on a CSR column had no impact on backpressure or resolution of a standard CL mixture.
Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada.
Clara M
CM
Olivia
Peta-Gaye G
PG
Burnett
Denis P
DP
Okinyo-Owiti
Jianheng
J
Shen
Martin J T
MJ
Reaney
D000097:Acetonitriles / Q000737:chemistry; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation* / Q000379:methods*; D019597:Flax / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D010456:Peptides, Cyclic; D010936:Plant Extracts; D010940:Plant Proteins; D014867:Water; D000432:Methanol; C032159:acetonitrile