Measurement of insulin sensitivity in skeletal muscle in vitro.
Glucose disposal in skeletal muscle is a major target for insulin action and assessment of insulin-regulated glucose uptake under in vitro conditions allows the direct determination of insulin sensitivity in this organ. For this purpose, a variety of muscle preparations from different parts of the body can be used. We describe here a detailed protocol for using epitrochlearis muscle strips and additionally for using primary skeletal muscle cells.
Paul-Langerhans-Group Integrative Physiology, German Diabetes Center, Düsseldorf, Germany.
Henrike
H
Sell
Jørgen
J
Jensen
Juergen
J
Eckel
D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance; D051379:Mice; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D051381:Rats
D007328:Insulin; D005947:Glucose