Flavobacterium aciduliphilum sp. nov., isolated from freshwater, and emended description of the genus Flavobacterium.
A Gram-stain-negative, rod-shaped, non-motile and yellow-pigmented bacterial strain, designated strain JJ013(T), was isolated from an artificial lake in Jeollabuk-do, South Korea, and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain JJ013(T) indicated that the isolate belonged to the family Flavobacteriaceae and exhibited similarity levels of 96.6 % to the type strains of Flavobacterium cheonanense and Flavobacterium koreense and 96.5 % to the type strain of Flavobacterium chungnamense. Growth was observed at 20-30 °C and pH 5.0-7.0. The major cellular fatty acids of the novel strain were iso-C15 : 0 (27.5 %), iso-C15 : 1 G (17.8 %), iso-C17 : 0 3-OH (9.4 %) and iso-C15 : 0 3-OH (9.2 %). Flexirubin-type pigments were present. The DNA G+C content of strain JJ013(T) was 33.9 mol%, the major respiratory quinone was menaquinone-6 (MK-6) and the major polyamine was sym-homospermidine. The polar lipid profile of the strain JJ013(T) consisted of a phosphatidylethanolamine (PE), two unknown aminolipids (AL1-2), three unidentified lipid (L1-3) and an unknown glycolipid (GL). On the basis of the morphological and physiological properties and biochemical evidence presented, it is concluded that strain JJ013(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium aciduliphilum sp. nov. is proposed; the type strain is JJ013(T) ( = KACC 16594(T) = JCM 18211(T)). Since C15 : 0, which is known as a predominant fatty acid of the genus Flavobacterium was not detected in the novel strain and other reference strains, we propose an emended description of the genus Flavobacterium.
Department of Life Sciences, Chonbuk National University, Jeonju-si, 561-756, Republic of Korea.
Ji Young
JY
Kang
Department of Life Sciences, Chonbuk National University, Jeonju-si, 561-756, Republic of Korea.
Jeesun
J
Chun
Department of Life Sciences, Chonbuk National University, Jeonju-si, 561-756, Republic of Korea.
Kwang Yeop
KY
Jahng
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005417:Flavobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005618:Fresh Water / Q000382:microbiology*; D006017:Glycolipids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D011090:Polyenes / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000031:analogs & derivatives / Q000032:analysis; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D011090:Polyenes; D012336:RNA, Ribosomal, 16S; C007961:sym-homospermidine; D024482:Vitamin K 2; C017313:flexirubins; C042455:menaquinone 6; D013095:Spermidine