Protein and fat mobilization and associations with serum β-hydroxybutyrate concentrations in dairy cows.
The objective of this study was to obtain information on variation between dairy cows in muscle and fat tissue mobilization around parturition and to study the association between protein and fat mobilization and serum β-hydroxybutyrate (BHBA) concentrations (hyperketonemia) in this period. Thirty-four cows kept under similar conditions at a university dairy farm (no experimental treatments) were monitored from 4 wk before until 8 wk after calving. Mobilization of muscle protein was investigated by analysis of plasma 3-methylhistidine concentrations (3-MH, analyzed by a recently developed HPLC tandem mass spectrometry method) and ultrasound measurements of longissimus muscle thickness. Mobilization of fat tissue was monitored by serum nonesterified fatty acid (NEFA) concentrations and ultrasound measurements of backfat thickness. Large variation was observed between cows in onset and duration of periparturient protein and fat mobilization. Plasma 3-MH concentrations and muscle thickness profiles indicated that protein mobilization started, on average, before parturition and continued until approximately wk 4 of lactation. Serum NEFA concentrations and backfat thickness profiles showed that fat mobilization occurred from parturition until the end of the study. Thus, muscle protein mobilization occurred in advance of fat mobilization in most cows from this study. We hypothesized that this might be due to a prepartum amino acid deficiency in the absence of negative energy balance. The incidence of hyperketonemia in this study was 16/34 = 47%. With the exception of 3 cows defined as having severe hyperketonemia, cows with lower 3-MH concentrations had higher serum BHBA concentrations. A possible explanation for this observation might be that higher mobilization of protein around calving might restrict ketone body production due to the higher availability of glucogenic precursors in the period of most severe negative energy balance and highest fat mobilization. The validity of this hypothesis needs to be confirmed, but data from this study indicate that further research on the role of protein mobilization in the etiology of hyperketonemia in dairy cows is needed.
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, PO Box 80151, 3508 TD Utrecht, the Netherlands. Electronic address: sgavanderdrift@gmail.com.
S G A
SGA
van der Drift
Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, PO Box 80176, 3508 TD Utrecht, the Netherlands.
M
M
Houweling
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, PO Box 80151, 3508 TD Utrecht, the Netherlands.
J T
JT
Schonewille
Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, PO Box 80176, 3508 TD Utrecht, the Netherlands.
A G M
AGM
Tielens
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, PO Box 80151, 3508 TD Utrecht, the Netherlands.
R
R
Jorritsma
D020155:3-Hydroxybutyric Acid / Q000097:blood* / Q000502:physiology; D000273:Adipose Tissue / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D002417:Cattle / Q000097:blood / Q000378:metabolism / Q000502:physiology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D007662:Ketosis / Q000097:blood / Q000503:physiopathology / Q000662:veterinary; D007774:Lactation / Q000378:metabolism / Q000502:physiology; D008762:Methylhistidines / Q000097:blood; D009124:Muscle Proteins / Q000378:metabolism / Q000502:physiology*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology; D011247:Pregnancy
D005230:Fatty Acids, Nonesterified; D008762:Methylhistidines; D009124:Muscle Proteins; C028118:3-methylhistidine; D020155:3-Hydroxybutyric Acid