Quantification of the antioxidant activity in salt-stressed tissues.
Biochemical methods available for the measurement of antioxidant activity in salt-stressed tissues are reviewed, outlining the most important advantages and shortcomings of the methods. Here we consider commonly used methods for measuring total antioxidant capacity and phenolic content, ABTS and Folin-Ciocalteu's procedure, respectively. Moreover, we presented assays for determination of antioxidant enzymes activities: superoxide dismutase, catalase, and ascorbate peroxidase. This choice of methods enables us to elucidate a full profile of antioxidant activities, evaluating their effectiveness against various reactive oxygen species produced during salt stress.
Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia. draxy@imsi.rs
Jelena J Dragišić
JJ
Maksimović
Branka D
BD
Zivanović
D000975:Antioxidants / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D010944:Plants / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D054712:Salinity; D055049:Salt Tolerance / Q000502:physiology*; D013312:Stress, Physiological
D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts