Emerging applications of sperm, embryo and somatic cell cryopreservation in maintenance, relocation and rederivation of swine genetics.
Advances in porcine assisted reproductive technology (ART) make it possible to use cryopreserved sperm, embryos and somatic cells in the maintenance, relocation and regeneration of swine genetics. In this review, development of key application-limiting technology is discussed in each cell type, focusing on the efficiencies, ease of storage and transportation, and minimization of pathogen transmission. Methods to regenerate swine genetics and/or models using frozen sperm, embryos and somatic cells in combination with other porcine ARTs, such as in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and somatic cell nuclear transplantation (SCNT), are also discussed. The applications of these ARTs utilizing cryopreserved cells will greatly increase the efficiency as well as biosecurity for maintenance, relocation and rederivation of swine genetics/models.
Department of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA.
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Men
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Walters
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Nagashima
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Prather
D000818:Animals; D019976:Cloning, Organism; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D004622:Embryo, Mammalian; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D008297:Male; D053652:Nuclear Transfer Techniques / Q000662:veterinary; D027724:Reproductive Techniques, Assisted / Q000662:veterinary*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D020554:Sperm Injections, Intracytoplasmic / Q000662:veterinary; D034421:Sus scrofa / Q000235:genetics; D013552:Swine / Q000235:genetics*