[Effects of L-arginine on acid-base balance and thromboelastograph in rabbits after banked-blood transfusion].
OBJECTIVE
To investigate the effects of L-arginine (L-Arg) on acid-base balance and thromboelastograph (TEG) in rabbits after banked-blood  transfusion.


METHODS
Fifty New Zealand white rabbits, of which 20 were banked-blood donors, and the remaining 30 were randomly divided into 3  groups (n=10).


METHODS
hypovolemic models were made through blood loss by 20% of blood volume, and were retransfused with equal volume of  banked-blood (14 days) 30 min later. Normal saline 1.2 mL/kg was given intravenously 5 min before transfusion; Sham operation group: the rabbits only  received 25% L-Arg 300 mg/kg (1.2 mL/kg) intravenously at the same time-point as control group, without blood loss and banked-blood transfusion; L-Arg group: the rabbits received the same procedure as control group,equal volume of L-Arg was given as the control instead of normal saline. Blood gas  /electrolyte analyses were determined before (T0) and after blood loss (T1), immediately after banked-blood transfusion (T2), 60 min (T3) and 120 min  (T4) after banked-blood transfusion. TEG were checked only at T0 and T4.


RESULTS
In control group and L-Arg group, pH values were declined after  blood loss and banked-blood transfusion, and then increased after the first drop. The pH value of control group at T2 was significantly lower than that at  T0 and T1 (P<0.05). Base excess (BE) values continued to decrease from T0 to T4 of the three groups, which were significantly lower at T4 than at T0 in  sham operation group (P<0.05). As to the concentration of blood lactate (Lac),there were no significant differences between the three groups at T0. In  sham operation group and L-Arg group, Lac decreased from T0 to T4 (P<0.05), which was significantly lower than that of control group at the same time-point (P<0.05). The TEG had no significant difference within and among the groups at T0 and T4 (P>0.05).


CONCLUSIONS
L-Arg 300 mg/kg intravenous  injection can improve tissue perfusion in rabbits receiving banked-blood transfusion, while there was little effect on acid-base balance and TEG.
Department of Anesthesiology, Peking University People's Hospital, Beijing, China.
Xue
X
Li
Yi
Y
Feng
Fang
F
Pan
Xiao-ning
XN
Wang
D000136:Acid-Base Equilibrium; D000818:Animals; D001120:Arginine / Q000008:administration & dosage*; D001771:Blood Banks; D007275:Injections, Intravenous; D008297:Male; D011817:Rabbits; D013916:Thrombelastography; D065227:Transfusion Reaction
D001120:Arginine