Cell surface lectin array: parameters affecting cell glycan signature.
Among the "omics", glycomics is one of the most complex fields and needs complementary strategies of analysis to decipher the "glycan dictionary". As an alternative method, which has developed since the beginning of the 21st century, lectin array technology could generate relevant information related to glycan motifs, accessibility and a number of other valuable insights from molecules (purified and non-purified) or cells. Based on a cell line model, this study deals with the key parameters that influence the whole cell surface glycan interaction with lectin arrays and the consequences on the interpretation and reliability of the results. The comparison between the adherent and suspension forms of Chinese Hamster Ovary (CHO) cells, showed respective glycan signatures, which could be inhibited specifically by neoglycoproteins. The modifications of the respective glycan signatures were also revealed according to the detachment modes and cell growth conditions. Finally the power of lectin array technology was highlighted by the possibility of selecting and characterizing a specific clone from the mother cell line, based on the slight difference determination in the respective glycan signatures.
GLYcoDIAG, Université d'Orléans, Orléans cedex 2, France.
Ludovic
L
Landemarre
Perrine
P
Cancellieri
Eric
E
Duverger
D000818:Animals; D016466:CHO Cells; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D006001:Glycoconjugates / Q000737:chemistry / Q000378:metabolism; D054794:Glycomics; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D046228:Microarray Analysis; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*
D006001:Glycoconjugates; D037102:Lectins; D011134:Polysaccharides