Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated ampC β-lactamase genes.
A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.
Creighton University School of Medicine Department of Medical Microbiology and Immunology Center for Research in Anti-Infectives and Biotechnology, Omaha, Nebraska, USA.
Chelsie N
CN
Geyer
Mark D
MD
Reisbig
Nancy D
ND
Hanson
D001426:Bacterial Proteins / Q000235:genetics*; D001431:Bacteriological Techniques / Q000379:methods*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D010957:Plasmids; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics*
D001426:Bacterial Proteins; C048795:AmpC beta-lactamases; D001618:beta-Lactamases