Monoclonal antibodies to DIPA: a novel binding partner of p120-catenin isoform 1.
The coiled-coil domain-containing delta-interacting protein A (DIPA) is a transcription factor implicated in developmental regulation. DIPA is the first protein discovered to selectively interact with the p120-catenin (p120) isoform 1, an alternatively spliced form of p120 expressed preferentially in mesenchymal cells. Although a small fraction of p120 can be observed in the nucleus under certain circumstances, the vast majority of it associates with classical cadherins at adherens junctions. We observed for the first time that a discrete fraction of DIPA exists at cell-cell junctions, in addition to its predominantly nuclear localization. Thus, the p120-DIPA interaction may regulate cell signaling and/or transcriptional events, as has been described previously for β-catenin and the LEF/TCF transcription factor family. To facilitate further study of DIPA and to determine the physiological relevance of its interaction with p120, we have generated and characterized a panel of five DIPA-specific monoclonal antibodies (MAbs) that function in immunoblotting, immunoprecipitation, and immunofluorescence assays.
Department of Cancer Biology, Vanderbilt University Medical Center, 2220 Pierce Avenue, Nashville, TN 37232, USA.
Nicholas O
NO
Markham
Tracy
T
Cooper
Matthew
M
Goff
Erin M
EM
Gribben
Robert H
RH
Carnahan
Albert B
AB
Reynolds
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000737:chemistry*; D000918:Antibody Specificity; D015153:Blotting, Western; D051177:Catenins / Q000378:metabolism*; D002460:Cell Line; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D055785:Gene Knockdown Techniques; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000737:chemistry*; D047468:Immunoprecipitation; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D034622:RNA Interference; D051381:Rats; D012097:Repressor Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000095002:Delta Catenin
D048868:Adaptor Proteins, Signal Transducing; D058846:Antibodies, Monoclonal, Murine-Derived; C101409:CCDC85B protein, human; D051177:Catenins; D007074:Immunoglobulin G; D020033:Protein Isoforms; D012097:Repressor Proteins; D000095002:Delta Catenin