Grape seed extract neutralizes the effects of Cerastes cerastes cerastes post-synaptic neurotoxin in mouse diaphragm.
This work was undertaken to investigate the toxic activity of the post-synaptic neurotoxic fraction isolated from the venom of the Egyptian sand viper (Cerastes cerastes cerastes), and the ability of grape seed extract to antagonize this effect produced in sublethally intoxicated mice, with an emphasis on ultrastructural features. Light and transmission electron microscopy of diaphragms of intoxicated mice showed myonecrosis, myofiber hypercontraction, sarcomere disorganization, and mitochondrial damage. Alterations in motor neurons and axon terminals were also observed. The toxic activities of C. cerastes cerastes neurotoxin were inhibited by administrating grape seed extract, either before or after intoxication, showing that grape seed extract has protective and therapeutic potential to be used as antivenom.
Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt. yomna_ia@yahoo.com
Yomna Ibrahim
YI
Mahmoud
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D000997:Antivenins / Q000494:pharmacology / Q000627:therapeutic use*; D003964:Diaphragm / Q000187:drug effects* / Q000378:metabolism; D056604:Grape Seed Extract / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects / Q000473:pathology; D009046:Motor Neurons / Q000187:drug effects / Q000473:pathology; D009210:Myofibrils / Q000187:drug effects / Q000473:pathology; D009498:Neurotoxins; D017729:Presynaptic Terminals / Q000187:drug effects / Q000473:pathology; D012518:Sarcomeres / Q000187:drug effects / Q000473:pathology; D014757:Viper Venoms / Q000494:pharmacology / Q000633:toxicity*; D017819:Viperidae
D000975:Antioxidants; D000997:Antivenins; D056604:Grape Seed Extract; D009498:Neurotoxins; D014757:Viper Venoms