[Analysis of polymorphism on ribosomal internal transcribed spacer 2 in four species of sandflies with PCR-RFLP].
Internal transcribed spacer 2 region in 4 species of sandflies from China (Phlebotomus chinensis, Ph. wui, Ph. longiductus and Ph. alexandri) was genotyped by PCR-RFLP. Four kinds of RFLP band patterns were created by Dra I, that were A 450 bp, B 154 bp/127 bp/93 bp/66 bp/10 hp, C 311 bp/80 bp/60 bp, and D 353 bp/300 bp/160 bp/58 bp. There were identical pattern types in various sandflies, AA in Ph. chinensis, BB in Ph. alexandri, CC in Ph. wui, and DD in Ph. longiductus. The results indicated that PCR-RFLP can be used to identify the four species of Phlebotomus in China.
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
Deng-An
DA
Gu
Yi
Y
Zhang
Qin-Xian
QX
Lan
D000818:Animals; D002681:China; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011576:Psychodidae / Q000145:classification / Q000235:genetics*; D013045:Species Specificity
D021903:DNA, Ribosomal Spacer