CD28-B7 interaction modulates short- and long-lived plasma cell function.
The interaction of CD28, which is constitutively expressed on T cells, with B7.1/B7.2 expressed on APCs is critical for T cell activation. CD28 is also expressed on murine and human plasma cells but its function on these cells remains unclear. There are two types of plasma cells: short-lived ones that appear in the secondary lymphoid tissue shortly after Ag exposure, and long-lived plasma cells that mainly reside in the bone marrow. We demonstrate that CD28-deficient murine short- and long-lived plasma cells produce significantly higher levels of Abs than do their wild-type counterparts. This was owing to both increased frequencies of plasma cells as well as increased Ab production per plasma cell. Plasma cells also express the ligand for CD28, B7.1, and B7.2. Surprisingly, deficiency of B7.1 and B7.2 in B cells also led to higher Ab levels, analogous to Cd28(-/-) plasma cells. Collectively, our results suggest that the CD28-B7 interaction operates as a key modulator of plasma cell function.
Department of Microbiology and Immunology, Emory Vaccine Center, Yerkes National Primate Center, Emory University, Atlanta, GA 30329, USA.
Modesta N
MN
Njau
Jin Hyang
JH
Kim
Craig P
CP
Chappell
Rajesh
R
Ravindran
Leela
L
Thomas
Bali
B
Pulendran
Joshy
J
Jacob
D000818:Animals; D060887:B7 Antigens / Q000172:deficiency / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D018106:CD28 Antigens / Q000096:biosynthesis / Q000172:deficiency / Q000502:physiology*; D002460:Cell Line; D002470:Cell Survival / Q000276:immunology*; D016922:Cellular Senescence / Q000276:immunology*; D005260:Female; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010950:Plasma Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
D060887:B7 Antigens; D018106:CD28 Antigens