Transcriptome analysis of Drosophila neural stem cells.
In Drosophila, the central nervous system is populated by a set of asymmetrically dividing neural stem cells called neuroblasts. Neuroblasts are derived from epithelial or neuroepithelial precursors, and divide along their apico-basal axes to produce a large apical neuroblast and a smaller basal ganglion mother cell. The ganglion mother cell will divide once again to produce two post-mitotic neurons or glia. In this chapter we outline a method for labeling different types of neural precursors in the Drosophila central nervous system, followed by their extraction and processing for transcriptome analysis. This technique has allowed us to capture and compare the expression profiles of neuroblasts and neuroepithelial cells, resulting in the identification of key genes required for the regulation of self-renewal and differentiation.
The Gurdon Institute, University of Cambridge, Cambridge, UK.
Katrina S
KS
Gold
Andrea H
AH
Brand
D000818:Animals; D001921:Brain / Q000166:cytology; D002469:Cell Separation; D004331:Drosophila melanogaster / Q000166:cytology* / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D007814:Larva / Q000166:cytology / Q000235:genetics; D009339:Needles; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D048348:Reverse Transcription