Effect of an omega-3 lipid emulsion in reducing oxidative stress in a rat model of intestinal ischemia-reperfusion injury.
OBJECTIVE
The usefulness of omega-3 lipid emulsions has been extensively studied. The objectives of the present study were to examine the effect of an omega-3 lipid emulsion in reducing oxidative stress in a rat model of intestinal ischemia-reperfusion injury and the underlying mechanism.


METHODS
A total of 66 rats were divided into three dietary groups (lipid-free, soybean oil, and fish oil groups). Each animal was administered total parenteral nutrition for 3 days, followed by induction of intestinal ischemia for 100 min. Animals subjected to sham surgery served as the controls. Intestinal tissue and blood were harvested 6 and 12 h after the surgery, then, assessment of the histological damage score, plasma-related parameters, and statistical evaluation were performed.


RESULTS
The histological damage score in the intestinal tissues was significantly lower in the fish oil group than in the soybean oil group (P = 0.0121). The late-phase urinary level of 8-hydroxy-2-deoxyguanosine was also significantly lower in the fish oil group as compared with that in the other groups (P = 0.0267). Furthermore, the plasma level of high-mobility group box 1 protein was also significantly lower in the fish oil group as compared with that in the lipid-free group (P = 0.0398).


CONCLUSIONS
It appeared that intravenous administration of an omega-3 lipid emulsion prior to ischemia-reperfusion injury reduced the oxidative stress and severity of tissue damage. Modification of membrane fatty acids may serve as the mechanism underlying this reduction of tissue damage.
School of Medicine, Department of Pediatric Surgery, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Atsuhiro
A
Arisue
Naoki
N
Shimojima
Masayuki
M
Tomiya
Takayuki
T
Shimizu
Daisuke
D
Harada
Mitsuo
M
Nakayama
Hirofumi
H
Tomita
Masahiro
M
Shinoda
Minoru
M
Tanabe
Ikuro
I
Maruyama
Masaru
M
Mizuno
Tatsuo
T
Kuroda
Go
G
Wakabayashi
Yasuhide
Y
Morikawa
D000818:Animals; D004195:Disease Models, Animal; D005217:Fat Emulsions, Intravenous / Q000008:administration & dosage*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D007421:Intestine, Small / Q000098:blood supply*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010289:Parenteral Nutrition, Total; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000628:therapy*
D005217:Fat Emulsions, Intravenous; D015525:Fatty Acids, Omega-3