[Replication-competent γ-retrovirus Mo-MuLV expressing green fluorescent protein gene as an efficient tool for screening of inhibitors of retroviruses that use heparan sulfate as the primary cell receptor].
The effect of sulfated polysaccharides on the efficiency of infection of mouse embryonic fibroblast cell lines SC-1 and NIH-3T3 by replication-competent recombinant Moloney murine leukemia virus (Mo-MuLV) carrying the eGFP gene was investigated. It was shown that used polysaccharides have no cytostatic and cytotoxic effects on SC-1 and NIH 3T3 cells inthe concentrations from 0.01 to 100 μg/ml and have virucidal activity against Mo-MuLV. Polysaccharides in the indicated concentrations inhibit cell infection by Mo-MuLV, that prevents further expansion of viral infection. It was detected that sulfated polysaccharides are effective inhibitors of other retroviruses, including lentiviruses, that use heparan sulfate as cell receptors for non-specific binding.
O A
OA
Stepanov
M M
MM
Prokof'eva
C
C
Stocking
V P
VP
Varlamov
A N
AN
Levov
G A
GA
Vikhoreva
P V
PV
Spirin
S N
SN
Mikhaĭlov
V S
VS
Prasolov
D000818:Animals; D002460:Cell Line; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects / Q000821:virology; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006497:Heparan Sulfate / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D057166:High-Throughput Screening Assays; D006801:Humans; D016086:Lentivirus / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008979:Moloney murine leukemia virus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D011991:Receptors, Virus / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014161:Transduction, Genetic; D014779:Virus Replication / Q000187:drug effects*
D048271:Chitosan; D049452:Green Fluorescent Proteins; D006497:Heparan Sulfate; D011991:Receptors, Virus; C485184:enhanced green fluorescent protein