[Preparation of the monoclonal antibody against human Bocavirus VP2 protein].
OBJECTIVE
To express and purify HBoV VP2 protein, and the monoclonal antibody against HBoV VP2 protein was prepared with hybridoma technique.


METHODS
The HBoV VP2 cloned into vector pET-30a was expressed in E. coil. After purified by immobilized metal affinity chromatography, the BALB/c mouse was immunized with purified protein as antigen. The positive hybridoma cells were screened with hybridoma technique and ELISA assay. Isotype and titer of the monoclonal antibody were detected.


RESULTS
The recombinant HBoV VP2 protein was expressed and purified, and then the monoclonal antibody was obtained with hybridoma technique. The titer of the IgG monoclonal antibody was up to 1:4 x 10(5).


CONCLUSIONS
Monoclonal antibody against recombinant HBoV VP2 protein was prepared and the antibody titer was high. This work may provide a new method in rapid diagnosis and study of HBoV.
College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhi-Hui
ZH
Zhao
Peng-Hao
PH
Xue
Jian-Min
JM
Wei
Qian
Q
Zhang
Wen-Zhi
WZ
Zheng
Fen-Lian
FL
Ma
Wu-Mei
WM
Yuan
Li-Shu
LS
Zheng
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D056661:Human bocavirus / Q000276:immunology*; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D011994:Recombinant Proteins