Facile synthesis of colorimetric histone deacetylase substrates.
Here we report a simple procedure for generating colorimetric histone deacetylase (HDAC) substrates by solid-phase peptide synthesis based on racemization-free couplings of amino acid chlorides. We demonstrate the applicability of these substrates in HDAC assays.
Interfaculty Institute of Biochemistry, University of Tuebingen, Hoppe-Seyler-Str. 4, 72076 Tuebingen, Germany.
Alexander
A
Dose
Jan Oliver
JO
Jost
Antje C
AC
Spieß
Petra
P
Henklein
Michael
M
Beyermann
Dirk
D
Schwarzer
D001557:Benzenesulfonates / Q000138:chemical synthesis / Q000737:chemistry; D003124:Colorimetry; D056572:Histone Deacetylase Inhibitors / Q000138:chemical synthesis / Q000737:chemistry; D006655:Histone Deacetylases / Q000737:chemistry / Q000378:metabolism*; D009243:NAD / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques; D013379:Substrate Specificity
D001557:Benzenesulfonates; D056572:Histone Deacetylase Inhibitors; D009243:NAD; C001073:2,4-dinitrobenzenesulfonic acid; D006655:Histone Deacetylases