Effect of Pleurotus florida lectin (PFL) on arsenic-induced activities of splenocytes in rat.
The present study was undertaken to investigate the protective effect of Pleurotus florida lectin (PFL) against arsenic-induced cytotoxicity and oxidative damages in freshly isolated splenocytes of rodents. Our finding indicated that arsenic caused reduction in cell adhesion, morphological alterations, cell proliferation, nitro blue tetrazolium (NBT) index, superoxide dismutase (SOD) activity, catalase (CAT) activity and relative mRNA expression of SOD2 in relation to housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and increased production of nitric oxide (NO), protein carbonyl (PC) and lipid peroxidation levels (LPO) assembled to play key factors for cytotoxicity and oxidative stress. PFL normalized cellular damages and enhanced SOD production pathway relating to gene expression. Further studies are needed to address effective phytochemicals of the edible mushroom and their mechanism.
1Indian Veterinary Research Institute, Eastern Regional Station, Kolkata, India.
Tanmoy
T
Rana
Asit Kumar
AK
Bera
Debasis
D
Bhattacharya
Subhashree
S
Das
Diganta
D
Pan
Subrata Kumar
SK
Das
D000818:Animals; D001151:Arsenic / Q000633:toxicity; D001688:Biological Products / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D053938:DNA Fragmentation / Q000187:drug effects; D005656:Fungal Proteins / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000032:analysis / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000166:cytology
D001688:Biological Products; D005656:Fungal Proteins; D037102:Lectins; D020011:Protective Agents; D009569:Nitric Oxide; D010088:Oxidoreductases; D001151:Arsenic