Quantitative cryo-analytical scanning electron microscopy (CEDX): an important technique useful for cell-specific localization of salt.
Advances in the techniques required for the X-ray microanalysis of cryo-fixed, naturally hydrated plant tissues in the cryo-scanning electron microscope have reached the stage that accurate, cell-specific localization and quantification of the nutrient and toxic elements can be achieved. Advances are described in the successive processes of cryo-fixation, cryo-planing to produce flat surfaces, monitored minimal etching to reveal cell outlines, coating with aluminum, spectrum collection, and quantification by comparison with comparable frozen standard solutions of the elements.
Division of Plant Industry, CSIRO, Canberra, ACT, Australia. margaret.mccully@csiro.au
Margaret
M
McCully
Martin
M
Canny
D020285:Cryoelectron Microscopy / Q000295:instrumentation / Q000379:methods*; D004577:Electron Probe Microanalysis / Q000295:instrumentation / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D059828:Plant Cells / Q000502:physiology / Q000648:ultrastructure; D012492:Salts / Q000032:analysis* / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry
D012492:Salts; D012965:Sodium Chloride