In vitro anti-diabetic activity and chemical characterization of an apolar fraction of Morus alba leaf water extract.
The tea from the white mulberry (Morus alba L.) leaf is a worldwide known traditional medicine of type II diabetes. Here, we report the investigation of the dichloromethane-soluble fraction obtained in a 0.24% m/m yield from the hot water extract of mulberry leaves. A significant, dose-dependent activity was found by means of the 24-h glucose consumption of fully differentiated adipocytes both in the absence and presence of insulin. The fraction was characterized by HPLC-DAD, GC-MS and GC-FID. The main constituent (40.3% by means of GC-FID) was isolated and identified as loliolide by EIMS, HRESIMS and NMR spectroscopy. In the active fraction benzyl alcohol, ethyl benzoate, t-cinnamic acid, p-hydroxyacetophenone, t-coniferyl alcohol and synapil alcohol were also identified by GC-MS and quantified by GC-FID (0.7, 1.3, 1.5, 2.9, 7.5 and 2.6%, respectively).
Institute of Pharmacognosy, University of Szeged, Szeged, Hungary. hunyadi.a@pharm.u-szeged.hu
Attila
A
Hunyadi
Katalin
K
Veres
Balazs
B
Danko
Zoltan
Z
Kele
Edit
E
Weber
Anasztazia
A
Hetenyi
Istvan
I
Zupko
Tusty-Jiuan
TJ
Hsieh
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects; D000818:Animals; D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D031625:Morus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry
D001572:Benzofurans; D007004:Hypoglycemic Agents; D010936:Plant Extracts; C030425:loliolide