Inhibition of semen-derived enhancer of virus infection (SEVI) fibrillogenesis by zinc and copper.
Semen-derived enhancer of virus infection (SEVI), a naturally occurring peptide fragment of prostatic acid phosphatase, enhances HIV infectivity by forming cationic amyloid fibrils that aid the fusion of negatively charged virion and target cell membranes. Cu(II) and Zn(II) inhibit fibrillization of SEVI in a kinetic assay using the fibril-specific dye ThT. TEM suggests that the metals do not affect fibril morphology. NMR shows that the metals bind to histidines 3 and 23 in the SEVI sequence. ITC experiments indicate that SEVI forms oligomeric complexes with the metals. Dissociation constants are micromolar for Cu(II) and millimolar for Zn(II). Because the Cu(II) and Zn(II) concentrations that inhibit fibrillization are comparable with those found in seminal fluid the metals may modulate SEVI fibrillization under physiological conditions.
Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269-3125, USA.
Sarah R
SR
Sheftic
Jessica M
JM
Snell
Suman
S
Jha
Andrei T
AT
Alexandrescu
D000682:Amyloid / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry*; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D006639:Histidine / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D046529:Microscopy, Electron, Transmission; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology*
D000682:Amyloid; D010446:Peptide Fragments; D006639:Histidine; D003300:Copper; C526435:prostatic acid phosphatase (248-286), human; D017027:Protein Tyrosine Phosphatases; D015032:Zinc