Life-span extension by caloric restriction is determined by type and level of food reduction and by reproductive mode in Brachionus manjavacas (Rotifera).
We measured life span and fecundity of three reproductive modes in a clone of the monogonont rotifer Brachionus manjavacas subjected to chronic caloric restriction (CCR) over a range of food concentrations or to intermittent fasting (IF). IF increased life span 50%-70% for all three modes, whereas CCR increased life span of asexual females derived from sexually or asexually produced eggs, but not that of sexual females. The main effect of CR on both asexual modes was to delay death at young ages, rather than to prevent death at middle ages or to greatly extend maximum life span; in contrast CR in sexual females greatly increased the life span of a few long-lived individuals. Lifetime fecundity did not decrease with CCR, suggesting a lack of resource allocation trade-off between somatic maintenance and reproduction. Multiple outcomes for a clonal lineage indicate that different responses are established through epigenetic programming, whereas differences in life-span allocations suggest that multiple genetic mechanisms mediate life-span extension.
Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA 02543, USA.
Kristin E
KE
Gribble
David B Mark
DB
Welch
D000367:Age Factors; D000818:Animals; D031204:Caloric Restriction / Q000379:methods* / Q000706:statistics & numerical data; D044127:Epigenesis, Genetic; D005215:Fasting / Q000502:physiology; D005260:Female; D005504:Food Analysis / Q000379:methods*; D005508:Food Deprivation / Q000502:physiology*; D053208:Kaplan-Meier Estimate; D008017:Life Expectancy; D008136:Longevity / Q000235:genetics / Q000502:physiology; D012098:Reproduction / Q000502:physiology*; D012403:Rotifera; D012737:Sex Factors