Analysis of mitochondrial DNA damage using 8-hydroxy 2'deoxyguanosine on in vitro and in vivo exposure of biomaterials.
The present study was carried out to evaluate the effect of HAP-EVA, fibrin glue, HA-BG, Latex and Dental material on oxidative stress related mtDNA damage by in vitro and in vivo methods. In vivo studies of these biomaterials were carried out by implanting biomaterials (five materials) on animals for period of 1, 4, 12, 26 and 52 weeks. At the end of observations, animals were anesthetized, sacrificed and tissues surrounding the implanted materials were collected. Brain, bone and muscles were used for the extraction of mtDNA. Similarly mtDNA was extracted from the homogenate of fresh brain, bone and muscles on exposure to the physiological saline extract of all the above five biomaterials (In vitro). The extracted mtDNA were subjected to analyse the presence of 8-OHdG. The results of study indicated that there was no significant increase in the level of 8-OHdG and thereby does not influence on the GC-TA transversions.
Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology , Thiruvananthapuram 695 012, Kerala, India.
S K
SK
Tinu
V S Anjana
VS
Vaman
C S
CS
Geetha
P V
PV
Mohanan
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000737:chemistry* / Q000187:drug effects*; D003764:Dental Materials / Q000633:toxicity; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D015718:Fibrin Tissue Adhesive / Q000633:toxicity; D007840:Latex / Q000633:toxicity; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D019736:Prostheses and Implants / Q000009:adverse effects; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D001672:Biocompatible Materials; D004272:DNA, Mitochondrial; D003764:Dental Materials; D015718:Fibrin Tissue Adhesive; D007840:Latex; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine