Formulation and shelf life stability of water-borne lecithin nanoparticles for potential application in dietary supplements field.
ABSTRACT The objective of the present investigation is to formulate commercial soybean lecithin as nanoparticles in solvent-free aqueous system for potential supplementary applications. A mechanical method, which involved two major steps, was used for that purpose. First, lecithin submicron particles (~ 0.5 μm) have been prepared by gradual hydration of lecithin powder using mechanical agitation. Finally, the size of these particles was further reduced to < 100 nm by using high-pressure microfluidization. The physical stability (appearance, particle size distribution, ζ-potential) and the chemical stability (lipid oxidation) of the dispersions carrying lecithin nanoparticles were assessed every 15 days during the 3-month shelf life period at two different temperatures. Results showed that the final particle size of lecithin in the freshly prepared aqueous dispersion was 79.8 ± 1.0 nm and the amount of peroxide detected was 3.5 ± 0.2 meq/kg lipid. At the end of the storage period, dispersions stored at 4°C exhibited physical and chemical stability as evident from the translucent appearance, the small change in particle size (84.1 ± 1.3 nm), and the small amount of generated peroxides (4.1 ± 0.2 meq/kg lipid). On the other hand, dispersions stored at 25°C were physically stable up to 60 days. Over that period, samples became turbid and the particle size increased to 145.0 ± 1.7 nm with a bimodal distribution pattern. This behavior was due to phospholipids (PLs) degradation and hydrolysis under acidic conditions, which proceeds faster at a relatively high temperature (25°C) than at (4°C). The outcome of this investigation may help in developing water-based dispersions carrying lecithin nanoparticles for dietary supplement of PLs.
Aroma and Flavor Chemistry Department, Food Industries and Nutrition Division, National Research Center, Cairo, Egypt. amr_edris@hotmail.com
Amr E
AE
Edris
D019587:Dietary Supplements; D004337:Drug Carriers; D004355:Drug Stability; D006801:Humans; D054709:Lecithins / Q000008:administration & dosage*; D015227:Lipid Peroxidation; D053758:Nanoparticles; D010316:Particle Size; D010743:Phospholipids / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry; D014867:Water
D004337:Drug Carriers; D054709:Lecithins; D010743:Phospholipids; D014867:Water