Characterization of Alloiococcus otitidis strains isolated from children with otitis media with effusion by Pulsed-Field Gel Electrophoresis.
OBJECTIVE
Alloiococcus otitidis is a slow growing organism which has been isolated in a few studies on patients with otitis media with effusion (OME). According to the literature review, there is no study about the molecular typing of A. otitidis. In this study, the characteristics of A. otitidis isolates from patients with OME were investigated via Pulsed-Field Gel Electrophoresis (PFGE) typing method.


METHODS
A total of 50 children with OME, who underwent myringotomy or who had an insertion of a ventilation tube, were included in this study. The isolates were identified to the species level as A. otitidis using standard biochemical methods, following which the amplification and sequencing of the 16S rRNA gene were carried out. The molecular characteristic of A. otitidis was investigated by PFGE technique.


RESULTS
Fifteen isolates of A. otitidis were identified in the middle ear fluid of the patients. All the isolates were susceptible to ampicillin, amoxicillin/clavulanate and fluoroquinolones. All of the 15 isolates were typed by PFGE method and were found to include 13 different PFGE types.


CONCLUSIONS
The current study, being the first reports on the molecular typing of A. otitidis by PFGE method, shows that A. otitidis is a heterogenic organism in Iranian children who have OME.
Department of Microbiology and Clinical Microbiology Research Center, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran.
Seyed Sajjad
SS
Khoramrooz
Akbar
A
Mirsalehian
Hossein
H
Imaneini
Fereshteh
F
Jabalameli
Asghar
A
Sharifi
Marzieh
M
Aligholi
Ebrahim
E
Razmpa
Babak
B
Saedi
Pedram
P
Borghaei
Morovat
M
Taherikalani
Mohammad
M
Emaneini
D019980:Amoxicillin-Potassium Clavulanate Combination / Q000627:therapeutic use; D000667:Ampicillin / Q000627:therapeutic use; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D024841:Fluoroquinolones / Q000627:therapeutic use; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy; D006801:Humans; D008297:Male; D008876:Middle Ear Ventilation; D010034:Otitis Media with Effusion / Q000382:microbiology* / Q000601:surgery; D012336:RNA, Ribosomal, 16S / Q000378:metabolism
D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D012336:RNA, Ribosomal, 16S; D019980:Amoxicillin-Potassium Clavulanate Combination; D000667:Ampicillin