A short-term in vivo experimental model for Fuchs endothelial corneal dystrophy.
OBJECTIVE
We evaluated the in vivo functionality of a corneal endothelium tissue-engineered using corneal endothelial cells from human patients with Fuchs endothelial corneal dystrophy (FECD).


METHODS
A total of 15 healthy cats underwent full-thickness corneal transplantation. All transplants were of xenogeneic human origin and all grafts but two were tissue-engineered. In seven animals the graft corneal endothelium was tissue-engineered using cultured corneal endothelial cells from humans with FECD (TE-FECD). Two control animals were grafted with an endothelium engineered using cultured endothelial cells from normal eye bank corneas (TE-normal). Two controls received a native full-thickness corneal transplant, and four other controls were grafted with the stromal carrier only (without endothelial cells). Outcome parameters included graft transparency (0, opaque to 4, clear), pachymetry, optical coherence tomography, endothelial cell morphometry, transmission electron microscopy (TEM), and immunostaining of function-related proteins.


RESULTS
Seven days after transplantation, 6 of 7 TE-FECD grafts, all TE-normal grafts, and all normal native grafts were clear (transparency score >3), while all carriers-only grafts were opaque (score <1). The mean pachymetry was 772 ± 102 μm for TE-FECD, 524 ± 11 μm for TE-normal, 555 ± 48 for normal native, and 1188 ± 223 μm for carriers only. TEM showed subendothelial loose fibrillar material deposition in all TE-FECD grafts. The TE endothelium expressed Na(+)-K(+)/ATPase and Na(+)/HCO3(-).


CONCLUSIONS
Restoration of transparency and corneal thickness demonstrated that the TE-FECD grafts were functional in vivo. This novel FECD seven-day living model suggests a potential role for tissue engineering leading to FECD cell rehabilitation.
Maisonneuve-Rosemont Hospital Research Center, Montreal, Québec, Canada.
M Nour
MN
Haydari
Marie-Claude
MC
Perron
Simon
S
Laprise
Olivier
O
Roy
J Douglas
JD
Cameron
Stéphanie
S
Proulx
Isabelle
I
Brunette
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002415:Cats; D002452:Cell Count; D063171:Corneal Pachymetry; D016039:Corneal Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000502:physiology / Q000648:ultrastructure; D004728:Endothelium, Corneal / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D005125:Eye Banks; D005260:Female; D005642:Fuchs' Endothelial Dystrophy / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery*; D006801:Humans; D007429:Intraocular Pressure / Q000502:physiology; D008297:Male; D046529:Microscopy, Electron, Transmission; D008875:Middle Aged; D011183:Postoperative Complications / Q000175:diagnosis; D061251:Primary Cell Culture; D023822:Tissue Engineering / Q000379:methods*; D014183:Transplantation, Heterologous