Single-Cell Sampling and Analysis (SiCSA).
Single-cell sampling and analysis allows the determination of solute concentrations in individual cells and tissues. This is particularly important when studying a stress such as salinity, where the cell- and tissue-specific distribution of sodium and chloride may decide a plant's fate. In this chapter, some selected SiCSA methods are described in detail, and their advantages and possible pitfalls discussed. These methods include pressure-driven extraction of cell contents (cell sap sampling) and the analysis of extracted cell sap through picolitre osmometry (osmolality), energy-dispersive X-ray analysis (concentrations of Na, K, P, S, Cl, Ca), and microfluorometry (concentrations of, for example, nitrate and total amino acids).
School of Biology and Environmental Science, University College Dublin, Dublin, Ireland. Wieland02fricke@yahoo.co.uk
Wieland
W
Fricke
D000596:Amino Acids / Q000378:metabolism; D007477:Ions / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000379:methods; D009566:Nitrates / Q000378:metabolism; D009995:Osmosis / Q000502:physiology*; D059828:Plant Cells / Q000737:chemistry*; D054712:Salinity; D059010:Single-Cell Analysis / Q000379:methods*
D000596:Amino Acids; D007477:Ions; D009566:Nitrates