Glyoxalase 1 and glyoxalase 2 activities in blood and neuronal tissue samples from experimental animal models of obesity and type 2 diabetes mellitus.
The glyoxalase enzymes catalyse the conversion of reactive glucose metabolites into non-toxic products as a part of the cellular defence system against glycation. This study investigated changes in glyoxalase 1 and glyoxalase 2 activities and the development of diabetic complications in experimental animal models of obesity (Zucker fa/fa rats) and type 2 diabetes mellitus (Goto-Kakizaki rats). In contrast to Zucker rats, in Goto-Kakizaki rats the glyoxalase 1 activities in brain, spinal cord and sciatic nerve tissues were significantly reduced by 10, 32 and 36 %, respectively. Lower glyoxalase 1 activity in the neuronal tissues was associated with a higher blood glucose concentration and impaired endothelium-dependent relaxation to acetylcholine in aortic rings in Goto-Kakizaki rats. This study provides evidence for disturbed neuronal glyoxalase 1 activity under conditions of hyperglycaemia in the presence of impaired endothelium-dependent relaxation and cognitive function.
Latvian Institute of Organic Synthesis, Aizkraukles Str. 21, Riga, LV1006, Latvia. skapare@biomed.lu.lv
Elina
E
Skapare
Ilze
I
Konrade
Edgars
E
Liepinsh
Marina
M
Makrecka
Liga
L
Zvejniece
Baiba
B
Svalbe
Reinis
R
Vilskersts
Maija
M
Dambrova
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications / Q000201:enzymology* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D005951:Glucose Tolerance Test / Q000379:methods; D007328:Insulin / Q000097:blood / Q000378:metabolism; D007791:Lactoylglutathione Lyase / Q000097:blood* / Q000378:metabolism*; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D023421:Models, Animal; D009474:Neurons / Q000201:enzymology* / Q000378:metabolism; D009765:Obesity / Q000097:blood / Q000150:complications / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D011924:Rats, Zucker; D013869:Thiolester Hydrolases / Q000097:blood* / Q000378:metabolism*; D014664:Vasodilation; D000099301:Hydroxyacylglutathione Hydrolase
D000109:Acetylcholine; D001786:Blood Glucose; D007328:Insulin; D007791:Lactoylglutathione Lyase; D008055:Lipids; D013869:Thiolester Hydrolases; D000099301:Hydroxyacylglutathione Hydrolase