Structural changes of glucose oxidase upon interaction with gold-coated magnetic nano-particles.
Structural changes of glucose oxidase (GOx), as a typical protein, upon interaction with gold-coated magnetic nano-particles (GMNPs) were studied. GMNPs were characterized by UV-vis, FTIR and SEM. Due to GMNPs interaction, obvious changes in enzyme conformation and its secondary and tertiary structures were verified using intrinsic fluorescence and circular dichroism. Moreover, Zeta potential measurement showed GMNPs surface charge shift to more negative value due to interaction with GOx. Finally, the electrochemical results revealed that while the enzyme electron transfer rate on GMNPs is higher, this process on cysteamin-GMNPs is more reversible.
Laboratory of Microanalysis, Institute of Biochemistry & Biophysics, University of Tehran, P.O. Box 13145-1384 Tehran, Iran.
Khadijeh
K
Eskandari
Hedayatollah
H
Ghourchian
D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D059346:Magnets / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D013057:Spectrum Analysis
D006046:Gold; D005949:Glucose Oxidase