[H2O2 promotes neutrophil adherence and injury of human umbilical vein endothelial cells].
To explore the role of hydrogen peroxide (H2O2) in promoting  polymorphonuclear neutrophils adherence and injury of human umbilical vein endothelial cells (HUVECs), the ordinary optical microscope and scanning electron microscopy were used to observe the adherence and injury after HUVECs co-cultured with neutrophils pretreated by extracellular H2O2 (HUVECs and neutrophils co-culture without H2O2 pretreatment as control), and the adhesion rates of neutrophils were measured through cell count test. The percentages of HUVECs expressing intercellular adhesion molecule 1 (ICAM-1) and Apo2.7 were detected by flow cytometry. After being cocultured with the neutrophils pretreated by extracellular H2O2, HUVECs showed obvious injury changes, such as round or oval shape, shortened or disappeared microvilli, and membrane structural damage; The adhesion rate of neutrophils was (57.74 ± 9.18)%, which was significantly higher than that in control [(23.12 ± 6.43)%, P < 0.01, n = 8]; The percentages of HUVECs expressing ICAM-1 and Apo2.7 were (44.69 ± 1.52)% and (39.29 ± 1.81)% respectively, which were significantly higher than those in control [(21.79 ± 1.43)% and (9.79 ± 1.43)%] (P < 0.01, n = 8). The results suggest that extracellular H2O2 can promote the neutrophils adherence and injury of HUVECs.
Key Laboratory of Anhui Province for Infection and Immunology, Department of Immunology, Bengbu Medical College, Bengbu 233003, China. qzq7778@yahoo.com.cn
Zhong-Qing
ZQ
Qian
Xiao-Yan
XY
He
Jing-Zhu
JZ
Lv
Hong-Tao
HT
Wang
Fang
F
Fang
D000071478:APOBEC Deaminases; D002448:Cell Adhesion; D018920:Coculture Techniques; D003564:Cytidine Deaminase / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000648:ultrastructure; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology*
D009124:Muscle Proteins; D018799:Intercellular Adhesion Molecule-1; D006861:Hydrogen Peroxide; D000071478:APOBEC Deaminases; C120388:APOBEC2 protein, human; D003564:Cytidine Deaminase