In vitro antiviral activity of an alkaline trypsin from the digestive juice of Bombyx mori larvae against nucleopolyhedrovirus.
Alkaline trypsin protein of molecular mass 25,436 Da purified from the digestive juice of Bombyx mori larvae indicated strong antiviral activity against Bombyx mori nucleopolyhedrovirus (BmNPV) under in vitro conditions. Partial N-terminal amino acid sequence of the protein was determined and the cDNA was cloned based on the amino acid sequence. A homology search of the deduced amino acid sequence of the cDNA showed 55% identity with Helicoverpa armigera trypsin and the active site of this protein was completely conserved. Hence, the protein was designated B. mori trypsin (Bmtryp). The results suggest that Bmtryp, an insect digestive enzyme, can be a potential antiviral factor against BmNPV at the initial site of viral infection.
Seribiotech Research Laboratory, Carmelaram-Post, Kodathi, Bangalore, India. kmpvel@yahoo.com
Kangayam M
KM
Ponnuvel
Krishnappa
K
Nithya
Sasibhushan
S
Sirigineedi
Arvind K
AK
Awasthi
Minoru
M
Yamakawa
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology; D001483:Base Sequence; D012831:Bombyx / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000821:virology; D002851:Chromatography, High Pressure Liquid; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D023061:Gene Order; D007814:Larva / Q000201:enzymology / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000187:drug effects / Q000502:physiology*; D010802:Phylogeny; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014357:Trypsin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D000998:Antiviral Agents; D014357:Trypsin