The effect of novel magnetic nanoparticles on vascular endothelial cell function in vitro and in vivo.
Manufactured nanoparticles are currently used for many fields. However, their potential toxicity provides a growing concern for human health. In our previous study, we prepared novel magnetic nanoparticles (MNPs), which could effectively remove heavy metal ions and cationic dyes from aqueous solution. To understand its biocompatibility, we investigated the effect of the nanoparticles on the function of vascular endothelial cells. The results showed that the nanoparticles were taken up by human umbilical vein endothelial cells (HUVECs) and could inhibit cell proliferation at 400 μg/ml. An increase in nitric oxide (NO) production and endothelial nitric oxide synthase (eNOS) activity were induced, which companied with the decrease in caveolin-1 level. The endothelium in the aortic root was damaged and the NO level in serum was elevated after treated mice with 20mg/kg nanoparticles for 3 days, but it was integrated after treated with 5mg/kg nanoparticles. Meanwhile, an increase in eNOS activity and decrease in caveolin-1 level were induced in the endothelium. The data suggested that the low concentration of nanoparticles could not affect the function and viability of VECs. The high concentration of nanoparticles could inhibit VEC proliferation through elevation of the eNOS activity and NO production and thus present toxicity.
Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan 250100, China.
Le
L
Su
Lei
L
Han
Fei
F
Ge
Shang Li
SL
Zhang
Yun
Y
Zhang
Bao Xiang
BX
Zhao
Jing
J
Zhao
Jun Ying
JY
Miao
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D051242:Caveolin 1 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism
D051242:Caveolin 1; D058185:Magnetite Nanoparticles; D009569:Nitric Oxide; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; C496315:Nos3 protein, mouse