Characterization of vitellogenin and its derived yolk proteins in cloudy catshark (Scyliorhinus torazame).
Elasmobranchs (sharks and rays) exhibit unique reproductive characteristics and, in contrast to the situation in teleosts, very little is known about the identity, structure and physical characteristics of their egg yolk proteins. The aims of this study were to (1) detect and purify the vitellogenin (Vtg; egg yolk precursor) and yolk proteins (YPs) of the cloudy catshark (Scyliorhinus torazame), (2) examine the relationships between Vtg and YPs and (3) characterize and classify the deduced primary structure of the Vtg transcript (vtg). The apparent molecular weights of purified Vtg and putative Vtg-related YPs (lipovitellin: Lv, phosvitin: Pv) were determined by gel filtration and were ~560, >669 and ~58 kDa, respectively. Following SDS-PAGE, these purified products (i.e., Vtg, Lv and Pv) appeared as bands of ~210, ~110 and ~22 kDa, respectively. On Western blots, antisera against purified Vtg, Lv and Pv recognized the ~210 kDa Vtg band. Catshark Pv, in contrast to teleost Pvs, had a very low serine content. The catshark Vtg cDNA sequence (vtg) appeared to contain an open-reading frame consisting of domains encoding Lv, Pv and β'-component (β'-c). A phylogenetic analysis, with a consideration of genome duplication events, placed catshark vtg into the 'vtgAB type.' It is concluded that at least a single major type of Vtg protein, which is transcribed and translated from catshark vtgAB gene, is the precursor of three egg yolk proteins (Lv, Pv and β'-c) in catshark.
Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
Kodai
K
Yamane
Tomoki
T
Yagai
Osamu
O
Nishimiya
Rieko
R
Sugawara
Haruna
H
Amano
Toshiaki
T
Fujita
Naoshi
N
Hiramatsu
Takashi
T
Todo
Takahiro
T
Matsubara
Akihiko
A
Hara
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western / Q000662:veterinary; D002845:Chromatography / Q000662:veterinary; D002850:Chromatography, Gel / Q000662:veterinary; D016000:Cluster Analysis; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004527:Egg Proteins / Q000737:chemistry / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010774:Phosvitin / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012754:Sharks / Q000378:metabolism*; D014819:Vitellogenins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D004527:Egg Proteins; D014819:Vitellogenins; D010774:Phosvitin; C001549:lipovitellin