Selenium modulates oxidative stress-induced TRPM2 cation channel currents in transfected Chinese hamster ovary cells.
It has been recently reported that the essential antioxidant element selenium has protective effects on cytosolic Ca(2+)  levels in cell lines. However, the effects of selenium on like transient receptor potential melastatin 2 (TRPM2) in response to oxidative stress (H(2) O(2) ) are not well understood. We investigated the effects of selenium on H(2) O(2) -induced TRPM2 channel currents in the Chinese hamster ovary (CHO) cell line using patch-clamp and fura-2 fluorescence imaging techniques.
Department of Biophysics, Medical Faculty, Süleyman Demirel University, Isparta, Turkey. mustafanaziroglu@sdu.edu.tr
Mustafa
M
Nazıroğlu
Cemil
C
Özgül
Mustafa
M
Küçükayaz
Bilal
B
Çiğ
Simon
S
Hebeisen
Ramazan
R
Bal
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000378:metabolism; D016257:Fura-2 / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D018408:Patch-Clamp Techniques; D012643:Selenium / Q000494:pharmacology*; D050053:TRPM Cation Channels / Q000378:metabolism*; D014162:Transfection
D000975:Antioxidants; D005456:Fluorescent Dyes; D050053:TRPM Cation Channels; D006861:Hydrogen Peroxide; D012643:Selenium; D016257:Fura-2