Effects of pinocembrin on the initiation and promotion stages of rat hepatocarcinogenesis.
Pinocembrin (5, 7-dihydroxyflavanone) is a flavanone extracted from the rhizome of Boesenbergia pandurata. Our previous studies demonstrated that pinocembrin had no toxicity or mutagenicity in rats. We here evaluated its effects on the initiation and promotion stages in diethylnitrosamine-induced rat hepatocarcinogenesis, using short- and medium-term carcinogenicity tests. Micronucleated hepatocytes and liver glutathione-S-transferase placental form foci were used as end point markers. Pinocembrin was neither mutagenic nor carcinogenic in rat liver, and neither inhibited nor prevented micronucleus formation as well as GST-P positive foci formation induced by diethylnitrosamine. Interestingly, pinocembrin slightly increased the number of GST-P positive foci when given prior to diethylnitrosamine injection.
Department of Biochemistry and Center for Innovation in Chemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Charatda
C
Punvittayagul
Wilart
W
Pompimon
Hideki
H
Wanibuchi
Shoji
S
Fukushima
Rawiwan
R
Wongpoomchai
D000818:Animals; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity; D004052:Diethylnitrosamine / Q000633:toxicity; D044950:Flavanones / Q000494:pharmacology*; D051549:Glutathione S-Transferase pi / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology*; D008297:Male; D015162:Micronucleus Tests; D011230:Precancerous Conditions / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D044950:Flavanones; D004052:Diethylnitrosamine; C016063:pinocembrin; D051549:Glutathione S-Transferase pi; C496558:Gstp1 protein, rat