Activation of peripheral P2X receptors is sufficient to induce central sensitization in rat medullary dorsal horn nociceptive neurons.
Central sensitization and purinergic receptor mechanisms have been implicated as important processes in acute and chronic pain conditions following injury or inflammation of peripheral tissues. This study has documented that application of the P2X(1,2/3,3) receptor agonist αβ-meATP (100mM) to the rat tooth pulp induces central sensitization in medullary dorsal horn nociceptive neurons that is reflected in significant increases in mechanoreceptive field size and responses to noxious stimuli and decreased mechanical activation threshold. Furthermore, these responses can be blocked by pulp application of the P2X(1,2/3,3) antagonist TNP-ATP and also attenuated by medullary application of TNP-ATP. These results suggest that activation of P2X(1,2/3,3) receptors in orofacial tissues plays a critical role in producing central sensitization in medullary dorsal horn nociceptive neurons.
Faculty of Dentistry, Department of Oral Physiology, University of Toronto, Toronto, Ontario, Canada.
Pavel S
PS
Cherkas
Jonathan O
JO
Dostrovsky
Barry J
BJ
Sessle
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D003782:Dental Pulp / Q000187:drug effects / Q000502:physiology; D008297:Male; D009619:Nociceptors / Q000378:metabolism*; D010812:Physical Stimulation; D020671:Posterior Horn Cells / Q000502:physiology*; D058911:Purinergic P2X Receptor Agonists / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D058469:Receptors, Purinergic P2X / Q000378:metabolism*; D014275:Trigeminal Caudal Nucleus / Q000166:cytology / Q000502:physiology*
D058911:Purinergic P2X Receptor Agonists; D058469:Receptors, Purinergic P2X; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate