The complete genome sequence of Natrinema sp. J7-2, a haloarchaeon capable of growth on synthetic media without amino acid supplements.
Natrinema sp. J7-2 is an extreme haloarchaeon capable of growing on synthetic media without amino acid supplements. Here we report the complete genome sequence of Natrinema sp. J7-2 which is composed of a 3,697,626-bp chromosome and a 95,989-bp plasmid pJ7-I. This is the first complete genome sequence of a member of the genus Natrinema. We demonstrate that Natrinema sp. J7-2 can use gluconate, glycerol, or acetate as the sole carbon source and that its genome encodes complete metabolic pathways for assimilating these substrates. The biosynthetic pathways for all 20 amino acids have been reconstructed, and we discuss a possible evolutionary relationship between the haloarchaeal arginine synthetic pathway and the bacterial lysine synthetic pathway. The genome harbors the genes for assimilation of ammonium and nitrite, but not nitrate, and has a denitrification pathway to reduce nitrite to N(2)O. Comparative genomic analysis suggests that most sequenced haloarchaea employ the TrkAH system, rather than the Kdp system, to actively uptake potassium. The genomic analysis also reveals that one of the three CRISPR loci in the Natrinema sp. J7-2 chromosome is located in an integrative genetic element and is probably propagated via horizontal gene transfer (HGT). Finally, our phylogenetic analysis of haloarchaeal genomes provides clues about evolutionary relationships of haloarchaea.
College of Life Sciences, Wuhan University, Wuhan, Hubei, People's Republic of China.
Jie
J
Feng
Bin
B
Liu
Ziqian
Z
Zhang
Yan
Y
Ren
Yang
Y
Li
Fei
F
Gan
Yuping
Y
Huang
Xiangdong
X
Chen
Ping
P
Shen
Lei
L
Wang
Bing
B
Tang
Xiao-Feng
XF
Tang
D000596:Amino Acids / Q000096:biosynthesis / Q000494:pharmacology*; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D053898:Biosynthetic Pathways / Q000187:drug effects / Q000235:genetics; D050260:Carbohydrate Metabolism / Q000187:drug effects / Q000235:genetics; D002244:Carbon / Q000378:metabolism / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology*; D020745:Genome, Archaeal / Q000235:genetics*; D006216:Halobacteriaceae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism / Q000494:pharmacology; D010802:Phylogeny
D000596:Amino Acids; D019843:Archaeal Proteins; D003470:Culture Media; D002244:Carbon; D009584:Nitrogen