Effect of fibronectin on HBV infection in primary human fetal hepatocytes             in vitro.
This study was carried out to investigate the effect of fibronectin (FN)             on primary cultured human fetal hepatocytes infected by hepatitis B virus (HBV)             in vitro. Human fetal hepatocytes infected by HBV were cultured on culture dishes             coated with FN in vitro. HBsAg and HBeAg in supernatants were detected by ELISA,             HBcAg in nuclei by immunohistochemistry, HBV DNA by fluorescent quantitative PCR             and covalently closed circular DNA (cccDNA) by nested PCR. The results were compared             with cells cultured on dishes that were not coated with FN. Positivity for HBsAg             and HBeAg in supernatants appeared from day 5 on the non-coated dishes, while             positivity for HBsAg and HBeAg appeared from day 1 on the coated dishes. Positivity             for HBcAg in the nuclei on the non‑coated dishes was displayed from day 2 with             a positive rate of 15%, while positivity for HBcAg on the coated dishes was displayed             from day 1 with a positive rate of >90%. HBV DNA in cells on the non-coated             dishes was detected from day 4, while that on the coated dishes was detected from             day 1. The non-coated dishes were positive for cccDNA from day 8, while the coated             dishes were positive from day 2. FN coating is capable of accelerating HBV infection             in primary cultured fetal hepatocytes in vitro, increasing the duration of HBV             infection and the number of infected cells.
Department of Infectious Diseases, Anning Branch of Lanzhou General Hospital, Lanzhou Military Area Command, Lanzhou, PR China.
Fang
F
Wang
Xiaogang
X
Zhang
Jing
J
Zhang
Ling
L
Hui
Yuming
Y
Wang
Xiaohong
X
Wang
D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000494:pharmacology*; D006512:Hepatitis B Core Antigens / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics*; D022781:Hepatocytes / Q000166:cytology / Q000821:virology*; D006801:Humans; D016133:Polymerase Chain Reaction; D053586:Virus Internalization / Q000187:drug effects*
D004279:DNA, Viral; D005353:Fibronectins; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens