Stability and antioxidant activity of gossypol derivative immobilized on N-polyvinylpyrrolidone.
The objective of this study is analysis of stability and antioxidant and antiradical activities of the gossypol derivative - megosin conjugated with N-polyvinylpyrrolidone (PVP). The results of study have shown the greater stability of megosin+PVP than megosin in aqueous solution of wide range of pH. Here we also demonstrated that megosin+PVP, named rometin, possess high antioxidant activity in the same range as well known antioxidant trolox as determined by its ability to scavenge free ABTS(+) and DPPH radicals in vitro. In addition, megosin+PVP was able to prevent accumulation of products of lipid peroxidation (thiobarbituric acid reactive substances and diene conjugates) and lysophospholipids formation in mitochondria membranes caused by CCl(4)-induced oxidative stress in rat liver in vivo. Furthermore, megosin+PVP rescued mitochondrial functions, such as respiration and oxidative phosphorylation, which declined after CCl(4) administration. Thus we present that the conjugation of megosin to PVP increase its stability and remain antioxidant activity in vivo and in vitro.
Department of General Biophysics, University of Lodz, Banacha St. 12/16, 90-237 Lodz, Poland.
Maksim
M
Ionov
Nataliya V
NV
Gordiyenko
Izabela
I
Zukowska
Elmira
E
Tokhtaeva
Olga A
OA
Mareninova
Nina
N
Baram
Khairulla
K
Ziyaev
Kuralbay
K
Rezhepov
Maria
M
Zamaraeva
D000818:Animals; D000976:Antipain; D052160:Benzothiazoles / Q000737:chemistry; D001713:Biphenyl Compounds / Q000737:chemistry; D002251:Carbon Tetrachloride / Q000633:toxicity; D004355:Drug Stability; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology*; D006072:Gossypol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D008099:Liver / Q000166:cytology; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010851:Picrates / Q000737:chemistry; D011205:Povidone / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013451:Sulfonic Acids / Q000737:chemistry; D014867:Water / Q000737:chemistry
D052160:Benzothiazoles; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D013451:Sulfonic Acids; D014867:Water; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D000976:Antipain; C026676:gossypol beta-aminoethyl sodium sulfate; D002251:Carbon Tetrachloride; C004931:1,1-diphenyl-2-picrylhydrazyl; D011205:Povidone; D006072:Gossypol