Quick regulation of mRNA functions by a few seconds of photoirradiation.
3-Cyanovinylcarbazole nucleoside, which effectively photocrosslinks to the pyrimidine base in complementary RNA strands, was incorporated into antisense oligonucleotides, and we evaluated the photoreactivity and the sequence selectivity to mutated K-ras oligoRNAs, as well as the regulation of the function of K-ras mRNA. We demonstrated that the reverse transcription and the translation activity of K-ras mRNA were quickly suppressed by a few seconds of photoirradiation with the addition of the photoresponsive antisense ODN.
School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan.
Atsuo
A
Shigeno
Takashi
T
Sakamoto
Yoshinaga
Y
Yoshimura
Kenzo
K
Fujimoto
D002227:Carbazoles / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D006801:Humans; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D055668:Photochemical Processes; D016283:Proto-Oncogene Proteins p21(ras) / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011743:Pyrimidines / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D002227:Carbazoles; D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D011743:Pyrimidines; D012333:RNA, Messenger; D016283:Proto-Oncogene Proteins p21(ras); C030986:pyrimidine