β-elemene acts as an antitumor factor and downregulates the expression             of survivin, Bcl-xL and Mta-1.
β-elemene, a non-cellular antineoplastic agent, may be used to effectively             inhibit the growth and proliferation of various types of tumor cells by inhibiting             the nucleic acid synthesis or inducing their apoptosis and differentiation. The             aim of this study was to investigate the expression, as well as the effects, of             Mta-1, survivin and Bcl-xL in T24 bladder cancer cells following β-elemene treatment.             The expression of the three proteins in T24 cells following β-elemene treatment             was analyzed by immunocytochemistry staining and western blot analysis. The survival             rate and apoptosis of T24 cells following β-elemene treatment was detected by             MTT assay and TUNEL staining. We analyzed the internal corelations between apoptosis-associated             genes, tumor metastasis-associated genes (cancer genes) and cell apoptosis, and             investigated the mechanism of action by which β-elemene induces the apoptosis             of T24 cells at a molecular level. These results provide scientific evidence for             further study on the anticancer effect of β-elemene in carcinoma of the urinary             bladder. In this study, it is shown that β-elemene downregulates the expression             of survivin, Bcl-xL and Mta-1 in tumor cells. The apoptosis of T24 cells is dependent             on the dosage and length of incubation time of β-elemene.
Department of Urology, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China.
Xian
X
Chen
Yi
Y
Wang
Hongmei
H
Luo
Zhigang
Z
Luo
Tao
T
Zhang
Ning
N
Yang
Xiangyang
X
Long
Huang
H
Xie
Weibing
W
Qiu
Biao
B
Zhang
Jun
J
Ding
Luoyan
L
Yang
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects*; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D012717:Sesquiterpenes / Q000494:pharmacology*; D000077022:Survivin; D015534:Trans-Activators; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000473:pathology; D051020:bcl-X Protein / Q000378:metabolism*
D000970:Antineoplastic Agents; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; C089320:MTA1 protein, human; D012097:Repressor Proteins; D012717:Sesquiterpenes; D000077022:Survivin; D015534:Trans-Activators; D051020:bcl-X Protein; C445979:beta-elemene; D006655:Histone Deacetylases