Standardized methods and quality control limits for agar and broth microdilution susceptibility testing of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum.
An international multilaboratory collaborative study was conducted to develop standard media and consensus methods for the performance and quality control of antimicrobial susceptibility testing of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum using broth microdilution and agar dilution techniques. A reference strain from the American Type Culture Collection was designated for each species, which was to be used for quality control purposes. Repeat testing of replicate samples of each reference strain by participating laboratories utilizing both methods and different lots of media enabled a 3- to 4-dilution MIC range to be established for drugs in several different classes, including tetracyclines, macrolides, ketolides, lincosamides, and fluoroquinolones. This represents the first multilaboratory collaboration to standardize susceptibility testing methods and to designate quality control parameters to ensure accurate and reliable assay results for mycoplasmas and ureaplasmas that infect humans.
Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA. waiteskb@uab.edu
Ken B
KB
Waites
Lynn B
LB
Duffy
Cécile M
CM
Bébéar
Anne
A
Matlow
Deborah F
DF
Talkington
George E
GE
Kenny
Patricia A
PA
Totten
Donald J
DJ
Bade
Xiaotian
X
Zheng
Maureen K
MK
Davidson
Virginia D
VD
Shortridge
Jeffrey L
JL
Watts
Steven D
SD
Brown
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D006801:Humans; D007391:International Cooperation; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards*; D019535:Mycoplasma hominis / Q000187:drug effects*; D009177:Mycoplasma pneumoniae / Q000187:drug effects*; D011786:Quality Control; D008973:Tenericutes; D016990:Ureaplasma urealyticum / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D003470:Culture Media