Genetic lineage tracing of beta cell neogenesis.
Genetic lineage tracing is an invaluable tool to demonstrate and measure neogenesis of beta cells from putative precursor cells. Cre-Lox recombination technology can be used for indelible labeling of a cohort of cells and following the fate of these cells and their progeny in animal models. Here, the combination is described of beta-galactosidase enzymatic staining with immunohistochemical staining to demonstrate labeled cells. This technique is performed in tissue cryosections.
Cell Differentiation Lab, Vrije Universiteit Brussel, Brussels, Belgium.
Iris
I
Mathijs
Isabelle
I
Houbracken
Luc
L
Bouwens
D000818:Animals; D019070:Cell Lineage; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007328:Insulin / Q000032:analysis; D050417:Insulin-Secreting Cells / Q000166:cytology*; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D013194:Staining and Labeling / Q000379:methods*; D013629:Tamoxifen / Q000008:administration & dosage; D001616:beta-Galactosidase / Q000032:analysis*
D007328:Insulin; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase