Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential.
OBJECTIVE
This study investigated the time-dependent remodeling and growth potential of porcine small intestine submucosa as a biomaterial for the reconstruction of pulmonary arteries in a lamb model.


METHODS
Left pulmonary arteries were partially replaced with small intestine submucosal biomaterial in 6 lambs. Two animals each were humanely killed at 1, 3, and 6 months. Computed tomographic angiography, macroscopic examination of the implanted patch, and microscopic analysis of tissue explants were performed.


RESULTS
All animals survived without complications. Patency and arborization of the pulmonary arteries were detected 6 months after implantation. There was no macroscopic narrowing or aneurysm formation in the patch area. The luminal appearance of the patch was similar to the intimal layer of the adjacent native pulmonary artery. Scanning electron microscopy showed that the luminal surface of the patch was covered by confluent cells. Immunohistochemical examination confirmed endothelialization of the luminal side of the patch in all of the explanted patches. The presence of smooth muscle cells in the medial layer was confirmed at all time points; however, expression of elastin, growth of the muscular layer, and complete degradation of patch material were detectable only after 6 months. The presence of c-Kit-positive cells suggests migration of multipotent cells into the patch, which may play a role in remodeling the small intestine submucosal biomaterial.


CONCLUSIONS
Our data confirmed that remodeling and growth potential of the small intestine submucosal biomaterial are time dependent. Additional experiments are required to investigate the stability of the patch material over a longer period.
Division of Pediatric Cardiac Surgery, Department of Cardiothoracic Surgery, Stanford University, Stanford, CA, USA.
Lorenzo
L
Boni
Fariba
F
Chalajour
Takashi
T
Sasaki
Radhika Lal
RL
Snyder
Walter D
WD
Boyd
R Kirk
RK
Riemer
V Mohan
VM
Reddy
D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers / Q000378:metabolism; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation / Q000295:instrumentation*; D042783:Endothelial Cells / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000254:growth & development / Q000637:transplantation*; D007421:Intestine, Small / Q000254:growth & development / Q000637:transplantation*; D008855:Microscopy, Electron, Scanning; D023421:Models, Animal; D039902:Multipotent Stem Cells / Q000378:metabolism / Q000648:ultrastructure; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000648:ultrastructure; D011474:Prosthesis Design; D011651:Pulmonary Artery / Q000000981:diagnostic imaging / Q000254:growth & development / Q000378:metabolism / Q000601:surgery* / Q000648:ultrastructure; D012756:Sheep; D013552:Swine; D013997:Time Factors; D014057:Tomography, X-Ray Computed; D014183:Transplantation, Heterologous; D014654:Vascular Patency
D015415:Biomarkers