A role for oxalic acid generation in ozone-induced signallization in Arabidopis cells.
Ozone (O(3) ) is an air pollutant with an impact increasingly important in our industrialized world. It affects human health and productivity in various crops. We provide the evidences that treatment of Arabidopsis thaliana with O(3)  results in ascorbate-derived oxalic acid production. Using cultured cells of A. thaliana as a model, here we further showed that oxalic acid induces activation of anion channels that trigger depolarization of the cell, increase in cytosolic Ca(2+)  concentration, generation of reactive oxygen species and cell death. We confirmed that O(3)  reacts with ascorbate in the culture, thus resulting in production of oxalic acid and this could be part of the O(3) -induced signalling pathways that trigger programmed cell death.
Université Paris Diderot, Sorbonne Paris Cité, Institut des Energies de Demain (IED), Paris, France. daniel.tran@univ-paris-diderot.fr
Daniel
D
Tran
Takashi
T
Kadono
Maria Lia
ML
Molas
Rafik
R
Errakhi
Joël
J
Briand
Bernadette
B
Biligui
Tomonori
T
Kawano
François
F
Bouteau
D000393:Air Pollutants / Q000378:metabolism; D000838:Anions / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D016923:Cell Death; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D019815:Oxalic Acid / Q000378:metabolism*; D010126:Ozone / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
D000393:Air Pollutants; D000838:Anions; D017382:Reactive Oxygen Species; D010126:Ozone; D019815:Oxalic Acid; D001205:Ascorbic Acid; D002118:Calcium