Nanocarriers for topical administration of resveratrol: a comparative study.
The trans-resveratrol (t-res), a non-flavonoid polyphenol extracted from different plants, has recently earned interest for application on the skin for different applications. In this work, the potential of nanocarriers, namely transfersomes and ethanol-containing vesicles, to deliver t-res into/through the skin was investigated. Thus, transfersomes with different surfactants, namely polysorbate 80 (Tw80), sodium cholate (SC) and sodium deossicholate (SDC) and ethanol-containing vesicles with different lipid composition, namely soy phosphatidylcholine (SPC) and cholesterol (chol), encapsulating t-res were prepared and characterized. The nanocarriers had a mean diameter ranging between 83 and 116 nm with a high t-res encapsulation efficiency (≥ 70%). Moreover, cytotoxicity as well as the inhibition of production of reactive oxygen species (ROS) and lipid peroxidation, following incubation of H(2)O(2)-stimulated human keratinocyte (HaCaT) with t-res, as free or encapsulated into the nanocarriers, were investigated. Only blank nanocarriers containing Tw80 or ethanol were cytotoxic and led to increase of ROS, but this effect was not observed when using nanocarriers encapsulating t-res. Finally, permeation studies on porcine skin carried out on Franz diffusion cells, showed that only ethanol-containing vesicles based SPC were able to promote t-res permeation through the skin.
Department of Pharmaceutical and Toxicological Chemistry, Faculty of Pharmacy, University of Naples Federico II, via Montesano 49, Naples, Italy.
Immacolata
I
Scognamiglio
Daniela
D
De Stefano
Virginia
V
Campani
Laura
L
Mayol
Rosa
R
Carnuccio
Gabriella
G
Fabbrocini
Fabio
F
Ayala
Maria Immacolata
MI
La Rotonda
Giuseppe
G
De Rosa
D000279:Administration, Cutaneous; D000818:Animals; D001682:Biological Availability; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D008315:Malondialdehyde / Q000378:metabolism; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D012869:Skin Absorption / Q000187:drug effects; D013267:Stilbenes / Q000008:administration & dosage* / Q000493:pharmacokinetics* / Q000494:pharmacology; D013499:Surface Properties; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000737:chemistry; D013552:Swine
D008081:Liposomes; D017382:Reactive Oxygen Species; D013267:Stilbenes; D013501:Surface-Active Agents; D000431:Ethanol; D008315:Malondialdehyde; D000077185:Resveratrol