Image-based analysis of light-grown seedling hypocotyls in Arabidopsis.
Time-resolved hypocotyl length measurements in seedlings have the potential to greatly aid genetic studies looking at light, hormone, and circadian regulation of cell expansion. Recently, several computer-based tools have been developed to quantify hypocotyl length during photomorphogenesis and early seedling development. Here we detail a method for quantifying Arabidopsis seedling hypocotyls in an image-based assay, focusing on light-grown seedlings responding to shade conditions.
Plant Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA. chory@salk.edu
Benjamin J
BJ
Cole
Joanne
J
Chory
D017360:Arabidopsis / Q000254:growth & development* / Q000528:radiation effects*; D018546:Hypocotyl / Q000254:growth & development* / Q000528:radiation effects*; D007091:Image Processing, Computer-Assisted; D008027:Light; D057054:Molecular Imaging / Q000379:methods*; D013997:Time Factors