Estimation of detection limits of a clinical fluorescence optical mammography system for the near-infrared fluorophore IRDye800CW: phantom experiments.
To evaluate if clinical fluorescence imaging of IRDye800CW is feasible on our fluorescence optical mammography system by estimating detection limits assessed by breast-cancer-simulating phantom experiments. Phantoms (2.1 cm(3), 0.9 cm(3)) with IRDye800CW concentrations of 0.5 to 120 nM were suspended in a 550 cm(3) measurement cup containing 507 surface-mounted source and detector fibers. The cup was filled with optical matching fluid containing IRDye800CW concentrations of 0, 5, 10, or 20 nM. Tomographic fluorescence images were acquired by exciting IRDye800CW at 730 nm; wavelengths above 750 nm were filtered. Signal intensities were calculated over a volume of interest corresponding to the size and location of the phantom in the reconstructed images. Correlations (R(2)) were calculated, and detection limits with associated upper 95% prediction interval were estimated. Between-day reproducibility was assessed with intraclass correlation coefficients (ICC). Fluorescent intensities were strongly correlated with phantom IRDye800CW concentrations (R(2)0.983 to 0.999). IRDye800CW detection limits ranged from 0.14 to 2.46 nM (upper 95% prediction limit 4.63 to 18.63 nM). ICC ranged from 0.88 to 1.00. The estimated detection limits for IRDye800CW were in the low-nanomolar range. These results support the start of clinical trials to evaluate the fluorescence optical mammography system using IRDye800CW labeled breast cancer targeting ligands.
University Medical Center Utrecht, Department of Radiology, Utrecht, The Netherlands. a.adams@umcutrecht.nl
Arthur
A
Adams
Jurgen E M
JE
Mourik
Marjolein
M
van der Voort
Paul C
PC
Pearlman
Tim
T
Nielsen
Willem P Th M
WP
Mali
Sjoerd G
SG
Elias
D001557:Benzenesulfonates; D001943:Breast Neoplasms / Q000473:pathology*; D003287:Contrast Media; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007211:Indoles; D007259:Infrared Rays; D008327:Mammography / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D019047:Phantoms, Imaging; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001557:Benzenesulfonates; D003287:Contrast Media; D005456:Fluorescent Dyes; C562366:IRDye 800CW; D007211:Indoles