Decreased erythrocyte NA+,K+-ATPase activity and increased plasma TBARS in prehypertensive patients.
The essential hypertension has been associated with membrane cell damage. The aim of the present study is investigate the relationship between erythrocyte Na(+),K(+)-ATPase and lipoperoxidation in prehypertensive patients compared to normotensive status. The present study involved the prehypertensive patients (systolic: 136 ± 7 mmHg; diastolic: 86.8 ± 6.3 mmHg; n = 8) and healthy men with normal blood pressure (systolic: 110 ± 6.4 mmHg; diastolic: 76.1 ± 4.2 mmHg; n = 8) who were matched for age (35 ± 4 years old). The venous blood samples of antecubital vein (5 mL) were collected into a tube containing sodium heparin as anticoagulant (1000 UI), and erythrocyte ghosts were prepared for quantifying Na(+),K(+)-ATPase activity. The extent of the thiobarbituric acid reactive substances (TBARS) was determined in plasma. The statistical analysis was carried out by Student's t-test and Pearson's correlation coefficient. A P < 0.05 was considered significant. The Na(+),K(+)-ATPase activity was lower in prehypertensive patients compared with normotensive subjects (4.9 versus 8.0 nmol Pi/mg protein/min; P < 0.05). The Na(+),K(+)-ATPase activity correlated negatively with TBARS content (r = -0.6; P < 0.05) and diastolic blood pressure (r = -0.84; P < 0.05). The present study suggests that Na(+),K(+)-ATPase activity reduction and elevation of the TBARS content may underlie the pathophysiological aspects linked to the prehypertensive status.
Department of Physical Education, Midwest State University, Campus Irati, 84500-000 PR, Irati, Brazil. crmalfatti@gmail.com
Carlos Ricardo Maneck
CR
Malfatti
Leandro Tibiriçá
LT
Burgos
Alexandre
A
Rieger
Cássio Luiz
CL
Rüdger
Janaína Angela
JA
Túrmina
Ricardo Aparecido
RA
Pereira
João Lang
JL
Pavlak
Luiz Augusto
LA
Silva
Raul
R
Osiecki
D016022:Case-Control Studies; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D008297:Male; D058246:Prehypertension / Q000097:blood* / Q000201:enzymology / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*
D017392:Thiobarbituric Acid Reactive Substances; D000254:Sodium-Potassium-Exchanging ATPase