Low predictive value of positive transplant perfusion fluid cultures for diagnosing postoperative infections in kidney and kidney-pancreas transplantation.
OBJECTIVE
Infection following transplantation is a cause of morbidity and mortality. Perfusion fluid (PF) used to preserve organs between recovery and transplantation represents a medium suitable for the growth of microbes. We evaluated the relevance of positive growth from PF sampled before the implantation of kidney or kidney-pancreas (KP) allografts.


METHODS
Between January 2007 and January 2011, 548 kidney/KP transplants were performed in our centre. A retrospective review of patient records with culture-positive PF was performed.


RESULTS
PF was received from 483 (88%) patients, of which 35 (7%, 95% CI 5.3% to 9.9%) were positive for bacteria (31/483, 6.4%, 95% CI 4.6% to 9.8%) and fungi (4/483, 0.8%, 95% CI 0.3% to 2.1%). Thirty-two of the 35 culture-positive PF (91.4%, 95% CI 77.6% to 97%) were considered insignificant. The remaining three patients developed sepsis postoperatively, which was considered to be possibly related to growth in PF; Escherichia coli in one and Klebsiella pneumoniae in two. Of the non-skin flora bacteria cultured from PF, six were resistant to the prophylactic antibiotic given intraoperatively, but only one developed infection postoperatively (E coli, resistant to the co-amoxiclav).


CONCLUSIONS
Significant attributable morbidity associated with PF-positive culture results was relatively rare. Culture of organisms other than Enterobacteriaceae or fungi are likely to represent contamination.
Department of Microbiology, Beaumont Hospital, Dublin, Ireland. mcotter@mater.ie
Meaghan P
MP
Cotter
Elizabeth
E
Smyth
Joanne
J
O'Gorman
Sarah
S
Browne
David P
DP
Hickey
Hilary
H
Humphreys
D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006801:Humans; D007239:Infections / Q000175:diagnosis*; D016030:Kidney Transplantation / Q000009:adverse effects*; D016035:Pancreas Transplantation / Q000009:adverse effects*; D010477:Perfusion / Q000009:adverse effects*; D011183:Postoperative Complications / Q000175:diagnosis* / Q000382:microbiology; D011237:Predictive Value of Tests; D012189:Retrospective Studies