Research resource: RNA-Seq reveals unique features of the pancreatic β-cell transcriptome.
The pancreatic β-cell is critical for the maintenance of glycemic control. Knowing the compendium of genes expressed in β-cells will further our understanding of this critical cell type and may allow the identification of future antidiabetes drug targets. Here, we report the use of next-generation sequencing to obtain nearly 1 billion reads from the polyadenylated RNA of islets and purified β-cells from mice. These data reveal novel examples of β-cell-specific splicing events, promoter usage, and over 1000 long intergenic noncoding RNA expressed in mouse β-cells. Many of these long intergenic noncoding RNA are β-cell specific, and we hypothesize that this large set of novel RNA may play important roles in β-cell function. Our data demonstrate unique features of the β-cell transcriptome.
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Gregory M
GM
Ku
Hail
H
Kim
Ian W
IW
Vaughn
Matthew J
MJ
Hangauer
Chang
C
Myung Oh
Michael S
MS
German
Michael T
MT
McManus
D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA; D059467:Transcriptome; D014365:Tryptophan Hydroxylase / Q000235:genetics / Q000378:metabolism
D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C487801:Tph1 protein, mouse; D014365:Tryptophan Hydroxylase