Downregulation of Pael-R expression in a Parkinson's disease cell model reduces apoptosis.
The pathogenesis of Parkinson's disease (PD), a common neurodegenerative disease, is poorly understood. Pael-R is a substrate of the Parkin protein, dysfunction of which can cause Pael-R protein accumulation, leading to cytotoxicity and dopaminergic neuronal apoptosis, causes of PD. In the present study, we induced PC12 cells to become dopaminergic neuron-like cells, and then suppressed Parkin expression in these cells to establish a PD cell model. We found that downregulating Pael-R gene expression in these PD model cells via RNA interference resulted in a reduction in the apoptotic rate. We conclude that downregulation of Pael-R gene expression is an effective approach for ameliorating the neuronal apoptosis caused by Parkin gene dysfunction.
Department of Neurology, Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Ting
T
Zou
Bo
B
Xiao
Jianguang
J
Tang
He
H
Zhang
Xiangqi
X
Tang
D000818:Animals; D000951:Antigens, Neoplasm; D017209:Apoptosis / Q000235:genetics*; D002454:Cell Differentiation; D002470:Cell Survival; D059290:Dopaminergic Neurons / Q000502:physiology; D015536:Down-Regulation / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D009419:Nerve Tissue Proteins; D000090682:Neuro-Oncological Ventral Antigen; D009474:Neurons; D016716:PC12 Cells; D034622:RNA Interference / Q000502:physiology; D016601:RNA-Binding Proteins; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism
D000951:Antigens, Neoplasm; C480827:GPR37 receptor, human; D009419:Nerve Tissue Proteins; D000090682:Neuro-Oncological Ventral Antigen; D016601:RNA-Binding Proteins; D043562:Receptors, G-Protein-Coupled; D044767:Ubiquitin-Protein Ligases; C111567:parkin protein