Evaluating the toxicity of hydroxyapatite nanoparticles in catfish cells and zebrafish embryos.
The toxicity of needle-(nHA-ND) and rod-shaped (nHA-RD) hydroxyapatite (HA) nanoparticles is evaluated in vitro on catfish B-cells (3B11) and catfish T-cells (28s.3) and in vivo on zebrafish embryos to determine if biological effects are similar to the effects seen in mammalian in vitro systems. Neither nHA-ND nor nHA-RD affect cell viability at concentrations of 10 to 300 μg mL(-1) . However, 30 μg mL(-1) needle-shaped nHA lower metabolic activity of the cells. Axial deformations are seen in zebrafish exposed to 300 μg mL(-1) needle shaped nHA after 120 h. For the first time, nHA is reported to cause zebrafish hatching delay. The lowest concentration (3 μg mL(-1) ) of both types of nHA cause the highest hatching inhibition and needle-shaped nHA exposed zebrafish exhibit the lowest hatch at 72 h post fertilization.
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798, Singapore.
Xinxin
X
Zhao
Kimberly J
KJ
Ong
James D
JD
Ede
James L
JL
Stafford
Kee Woei
KW
Ng
Greg G
GG
Goss
Say Chye Joachim
SC
Loo
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D002397:Catfishes; D004305:Dose-Response Relationship, Drug; D017886:Durapatite / Q000633:toxicity*; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D066298:In Vitro Techniques; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000633:toxicity*; D013601:T-Lymphocytes / Q000187:drug effects*; D015027:Zebrafish / Q000196:embryology*
D017886:Durapatite