Probable congenital transmission of reticuloendotheliosis virus caused by vaccination with contaminated vaccines.
Contaminated vaccine is one unexpected and potential origin of virus infection. In order to investigate the most likely cause of disease in a broiler breeder company of Shandong Province, all 17 batches of live-virus vaccines used in the affected flocks and 478 tissue samples were tested by dot-blot hybridization, nested PCR, and IFA. The results suggested the outbreak of disease was most probably due to the vaccination of REV-contaminated MD-CVI988/Rispens vaccines and ND-LaSota+IB-H120 vaccines. Furthermore, the REV was probably transmitted to the commercial chickens through congenital transmission.
Key Laboratory of Biological Product, College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong, People's Republic of China.
Kai
K
Wei
Zhenhong
Z
Sun
Shufen
S
Zhu
Wenlong
W
Guo
Pengcheng
P
Sheng
Zunmin
Z
Wang
Changliang
C
Zhao
Qingyou
Q
Zhao
Ruiliang
R
Zhu
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002478:Cells, Cultured; D002642:Chick Embryo / Q000166:cytology; D002645:Chickens / Q000276:immunology* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D004340:Drug Contamination; D005260:Female; D005347:Fibroblasts / Q000276:immunology / Q000821:virology; D019084:Fluorescent Antibody Technique, Indirect; D015686:Gene Products, env / Q000235:genetics; D008297:Male; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000276:immunology* / Q000635:transmission / Q000821:virology; D001356:Reticuloendotheliosis virus / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000821:virology; D014611:Vaccination; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D004279:DNA, Viral; D015686:Gene Products, env; D014765:Viral Vaccines