Characterization of nine microsatellite loci for the tree species Parapiptadenia rigida (Fabaceae-Mimosoideae) and their transferability.
Parapiptadenia rigida, locally known as angico, is a tropical tree common  in the semideciduous Brazilian forest. Its wood is naturally resistant to  insect attack and is useful for construction. Extracts from the tree have  medicinal properties. We characterized nine microsatellite loci for P.  rigida. Thirty-five alleles were detected in a sample of 45 individuals  from 3 different populations, with an average of 3.9 alleles per locus. The  average polymorphic information content ranged from 0.099 to 0.640. Observed  and expected heterozygosities varied from 0.111 to 0.489 and from 0.106 to  0.707, respectively. One locus exhibited significant deviation from Hardy-Weinberg  equilibrium and four pairs of loci showed significant linkage disequilibrium.  All nine primers were tested for cross-amplification in species from the  Fabaceae-Mimosoidea family, yielding a transferability success rate of 7 loci  in Stryphnodendron adstringens to 0 transferred loci in Pithecellobium  incuriale and Inga marginata. These microsatellites will be valuable  to study population genetics of this and other species where primer  transferability was detected.
Departamento de Agronomia, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
B F
BF
Costa
L A
LA
Rodrigues
E A
EA
Ruas
L B
LB
Souza
C F
CF
Ruas
B G
BG
Vieira
A R O
AR
Conson
P M
PM
Ruas
D056426:Genetic Loci / Q000235:genetics*; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D031298:Mimosa / Q000235:genetics*; D013045:Species Specificity; D014170:Transformation, Genetic; D014197:Trees / Q000235:genetics*