Lentisphaera marina sp. nov., and emended description of the genus Lentisphaera.
Two Gram-stain-negative, non-motile, non-pigmented cocci, designated IMCC11369(T) and IMCC11389, were isolated from surface seawater of the East Sea of Korea by high-throughput cultivation based on dilution to extinction. Strains IMCC11369(T) and IMCC11389 shared 99.9 % 16S rRNA gene sequence similarity and >86.3 % DNA-DNA relatedness, which suggested that they belong to the same genomic species. The isolates were most closely related to Lentisphaera araneosa HTCC2155(T) (99.0 % 16S rRNA gene sequence similarity). Phylogenetic analysis revealed that the isolates formed a robust cluster with L. araneosa HTCC2155(T). DNA-DNA relatedness values, however, showed that the isolates were distantly related to L. araneosa HTCC2155(T) (2.0-18.6 %), which suggested that they represent a separate genomic species in the genus Lentisphaera. The two isolates were phenotypically differentiated from their closest relative by several characteristics, including degradation of macromolecules and carbon source utilization. The DNA G+C content was 44.5-45.2 mol% and the predominant cellular fatty acids were C14 : 0, C16 : 1ω9c and C16 : 0. Strain IMCC11369(T) contained MK-7 as the respiratory quinone and phosphatidylethanolamine and an unknown lipid as the major polar lipids. On the basis of data obtained in this study, a novel species is proposed to accommodate the isolates, Lentisphaera marina sp. nov. The type strain is IMCC11369(T) ( = KCTC 23780(T) = NBRC 108776(T)).
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Ahyoung
A
Choi
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Seung-Jo
SJ
Yang
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Kwang-Hyun
KH
Rhee
Division of Biology and Ocean Sciences, Inha University, Incheon 402-751, Republic of Korea.
Jang-Cheon
JC
Cho
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7