Male-specific repeats in wild Bovidae.
In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination between closely related species or subspecies.
Department of Genetics and Reproduction, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic. cabelova@vri.cz
Katerina
K
Cabelova
Svatava
S
Kubickova
Halina
H
Cernohorska
Jiri
J
Rubes
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000145:classification / Q000235:genetics*; D019143:Evolution, Molecular; D005456:Fluorescent Dyes / Q000737:chemistry; D005828:Genetics, Population / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D061346:Laser Capture Microdissection / Q000379:methods / Q000662:veterinary; D008297:Male; D008677:Metaphase; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA / Q000379:methods / Q000662:veterinary*; D012737:Sex Factors; D013045:Species Specificity; D014998:Y Chromosome / Q000737:chemistry / Q000235:genetics*
D005456:Fluorescent Dyes