[Ginkgolide B promotes axonal growth of retina ganglion cells by anti-apoptosis in vitro].
One common feature of glaucoma, optic neuritis and some other optic nerve diseases is sustained and irreversible apoptosis of retinal ganglion cells (RGCs). Ginkgolide B is believed to protect neurons in brain and contribute to neurite outgrowth and synapse formation. The aim of the present study was to explore the effects of Ginkgo biloba extract (EGB761) and ginkgolide B on axonal growth of RCGs. Retina explants were cultured in three-dimensional tissue culture system, and the number and length of neurites were analyzed. Immunohistochemistry staining was performed to confirm that the neurite observed was axon of RGCs. TUNEL and activated caspase-3 staining were also applied to observe RGCs apoptosis. The result shows that neurites of RGCs treated with EGB761 or ginkgolide B were more and longer than those in control. The neurite is proved to be the axon of RGCs by immunostaining. Furthermore, compared with control group, RGCs treated with ginkgolide B showed decreased cellular apoptosis and inhibited caspase-3 activation. These results suggest ginkgolide B can promote RGCs axon growth by protecting RGCs against apoptosis.
Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Zhen-Yu
ZY
Wang
Xiao-Fen
XF
Mo
Xu-Hua
XH
Jiang
Xian-Fang
XF
Rong
Hua-Mao
HM
Miao
D000818:Animals; D017209:Apoptosis; D001369:Axons / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D020441:Ginkgo biloba; D046934:Ginkgolides / Q000494:pharmacology*; D007783:Lactones / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects; D009924:Organ Culture Techniques; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D012160:Retina; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000187:drug effects*; D000096983:Ginkgo Extract
D053148:Caspase 3; D046934:Ginkgolides; D007783:Lactones; D010936:Plant Extracts; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract; C045856:ginkgolide B; C505410:Casp3 protein, rat