Differential expression of class I small leucine-rich proteoglycans in an animal model of partial bladder outlet obstruction.
OBJECTIVE
Partial bladder outlet obstruction has been shown in a rat model to progress from inflammation to hypertrophy to fibrosis. Small leucine-rich proteoglycans are extracellular matrix components associated with collagen fibrillogenesis and resultant scar formation. Two such critical small leucine-rich proteoglycans are decorin and biglycan. We hypothesized that in keeping with other scar models, decorin would be down-regulated and biglycan would be up-regulated with the onset of fibrosis compared to sham.


METHODS
We challenged our hypothesis with female Fisher rats that underwent ligation of the bladder neck or sham surgery. Animals were sacrificed at 4, 8 and 12 weeks, and bladders were harvested. Frozen sections were stained for immunofluorescence for decorin and biglycan. mRNA expression for decorin and biglycan was analyzed using quantitative reverse transcriptase polymerase chain reaction.


RESULTS
All rats survived to specified experimental end points in good health. Immunofluorescent stains showed progressive down-regulation of decorin and up-regulation of biglycan during the 12-week course by 0.36 and 1.82-fold, respectively (p = 0.02 and p = 0.02), compared to shams. Quantitative real-time reverse transcriptase polymerase chain reaction confirmed these findings in 12-week specimens, showing a down-regulation of decorin by a factor of 0.45 (p = 0.02) and up-regulation of biglycan by a factor of 2.04-fold (p = 0.08).


CONCLUSIONS
We present the first identification to our knowledge of small leucine-rich proteoglycans in normal and abnormal bladder tissue, and their differential expression in the process of bladder fibrosis, consistent with experimental findings in other anatomical sites. Further investigation into small leucine-rich proteoglycan expression and regulation may allow for the development of new antifibrotic therapeutics.
Division of Urology, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada. conradm@ualberta.ca
Conrad C
CC
Maciejewski
Dariush
D
Honardoust
Edward E
EE
Tredget
Peter D
PD
Metcalfe
D000818:Animals; D058578:Biglycan / Q000096:biosynthesis*; D058575:Decorin / Q000096:biosynthesis*; D004195:Disease Models, Animal; D005260:Female; D051381:Rats; D011916:Rats, Inbred F344; D001748:Urinary Bladder Neck Obstruction / Q000378:metabolism*
D058578:Biglycan; D058575:Decorin