Dokdonella kunshanensis sp. nov., isolated from activated sludge, and emended description of the genus Dokdonella.
A gram-negative, aerobic, non-motile, non-spore-forming rod, designated DC-3(T), was isolated from activated sludge of a wastewater treatment plant in China. Comparative 16S rRNA gene sequence analysis showed that strain DC-3(T) belonged to the family Xanthomonadaceae and formed a lineage within the genus Dokdonella. Strain DC-3(T) shared the highest 16S rRNA gene sequence similarity with Dokdonella soli KIS28-6(T) (97.1 %) and Dokdonella fugitiva A3(T) (97.1 %). The G+C content of the genomic DNA was 71.5 mol%. The major respiratory quinone was Q-8 and the major fatty acids were iso-C17 : 1ω9c (31.6 %), iso-C15 : 0 (12.6 %), iso-C16 : 0 (21.3 %), iso-C17 : 0 (13.1 %) and iso-C11 : 0 3-OH (6.5 %), which supported the affiliation of strain DC-3(T) with the genus Dokdonella. DNA-DNA relatedness between strain DC-3(T) and its closest phylogenetic neighbours was <30 %. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain DC-3(T) from the recognized species of the genus Dokdonella. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain DC-3(T) represents a novel species of the genus Dokdonella, for which the name Dokdonella kunshanensis sp. nov. is proposed. The type strain is DC-3(T) ( = CCTCC AB 2011179(T)  = KACC 16511(T)). The description of the genus Dokdonella is also emended.
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Yi
Y
Li
College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095, PR China.|Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Jun
J
Zhang
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Qing
Q
Chen
Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
Guiqin
G
Yang
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Shu
S
Cai
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Jian
J
He
Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
Shungui
S
Zhou
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Shun-Peng
SP
Li
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater