Skin phototoxicity of cosmetic formulations containing photounstable and photostable UV-filters and vitamin A palmitate.
The aim of this study was to evaluate the in vitro skin phototoxicity of cosmetic formulations containing photounstable and photostable UV-filters and vitamin A palmitate, assessed by two in vitro techniques: 3T3 Neutral Red Uptake Phototoxicity Test and Human 3-D Skin Model In Vitro Phototoxicity Test. For this, four different formulations containing vitamin A palmitate and different UV-filters combinations, two of them considered photostable and two of them considered photounstable, were prepared. Solutions of each UV-filter and vitamin under study and solutions of four different combinations under study were also prepared. The phototoxicity was assessed in vitro by the 3T3 NRU phototoxicity test (3T3-NRU-PT) and subsequently in a phototoxicity test on reconstructed human skin model (H3D-PT). Avobenzone presented a pronounced phototoxicity and vitamin A presented a tendency to a weak phototoxic potential. A synergistic effect of vitamin A palmitate on the phototoxicity of combinations containing avobenzone was observed. H3D-PT results did not confirm the positive 3T3-NRU-PT results. However, despite the four formulations studied did not present any acute phototoxicity potential, the combination 2 containing octyl methoxycinnamate (OMC), avobenzone (AVB) and 4-methylbenzilidene camphor (MBC) presented an indication of phototoxicity that should be better investigated in terms of the frequency of photoallergic or chronic phototoxicity in humans, once these tests are scientifically validated only to detect phototoxic potential with the aim of preventing phototoxic reactions in the general population, and positive results cannot predict the exact incidence of phototoxic reactions in humans.
University of Sao Paulo, Faculty of Pharmaceutical Sciences of Ribeirao Preto, SP, Brazil. lorena@fcfrp.usp.br
Lorena R
LR
Gaspar
Julian
J
Tharmann
Patricia M B G
PM
Maia Campos
Manfred
M
Liebsch
D000826:Animal Testing Alternatives; D000818:Animals; D041702:BALB 3T3 Cells; D004396:Coloring Agents / Q000378:metabolism; D003358:Cosmetics / Q000633:toxicity*; D017484:Dermatitis, Phototoxic / Q000209:etiology*; D004224:Diterpenes; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D009499:Neutral Red / Q000378:metabolism; D000084562:Retinyl Esters; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects*; D013473:Sunscreening Agents / Q000633:toxicity*; D014466:Ultraviolet Rays; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000633:toxicity
D004396:Coloring Agents; D003358:Cosmetics; D004224:Diterpenes; D000084562:Retinyl Esters; D013473:Sunscreening Agents; D014801:Vitamin A; C014794:retinol palmitate; D009499:Neutral Red