Experimental resin cements containing bioactive fillers reduce matrix metalloproteinase-mediated dentin collagen degradation.
BACKGROUND
Collagen dentin matrix may represent a suitable scaffold to be remineralized in the presence of bioactive materials. The purpose of this study was to determine if experimental resin cements containing bioactive fillers may modulate matrix metalloproteinase-mediated collagen degradation of etched dentin.


METHODS
Human dentin beams demineralized using 10% phosphoric acid or 0.5 mol/L EDTA were infiltrated with the following experimental resins: (1) unfilled resin, (2) resin with Bioglass 45S5 particles (Sylc; OSspray Ltd, London, UK), and (3) resin with β-tricalcium phosphate-modified calcium silicate cement (HCAT-β) particles. The filler/resin ratio was 40/60 wt%. The specimens were stored in artificial saliva, and the determination of C-terminal telopeptide (ICTP) was performed by radioimmunoassay after 24 hours, 1 week, and 4 weeks. Scanning electron microscopic analysis of dentin surfaces after 4 weeks of storage was also executed.


RESULTS
Collagen degradation was prominent both in phosphoric acid and EDTA-treated dentin. Resin infiltration strongly reduced the MMP activity in demineralized dentin. Resin-containing Bioglass 45S5 particles exerted higher and more stable protection of collagen at all tested dentin states and time points. HCAT-β induced collagen protection from MMPs only in EDTA-treated specimens. Dentin remineralization was achieved when dentin was infiltrated with the resin cements containing bioactive fillers.


CONCLUSIONS
MMP degradation of dentin collagen is strongly reduced in resin-infiltrated dentin. The inclusion of Bioglass 45S5 particles exerted an additional protection of collagen during dentin remineralization.
Department of Dental Materials, School of Dentistry, University of Granada, Colegio Máximo, Campus de Cartuja, Granada, Spain. rosorio@ugr.es
Raquel
R
Osorio
Monica
M
Yamauti
Salvatore
S
Sauro
Thimoty F
TF
Watson
Manuel
M
Toledano
D000134:Acid Etching, Dental / Q000379:methods; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D017610:Calcium Compounds / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D002516:Ceramics / Q000737:chemistry; D003094:Collagen / Q000378:metabolism* / Q000648:ultrastructure; D024042:Collagen Type I / Q000032:analysis; D003804:Dentin / Q000378:metabolism* / Q000648:ultrastructure; D004492:Edetic Acid / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D020782:Matrix Metalloproteinases / Q000187:drug effects*; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000032:analysis; D010756:Phosphoric Acids / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry / Q000494:pharmacology*; D012464:Saliva, Artificial / Q000737:chemistry; D017640:Silicates / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D013997:Time Factors
D001672:Biocompatible Materials; D017610:Calcium Compounds; D002130:Calcium Phosphates; D024042:Collagen Type I; D061965:Matrix Metalloproteinase Inhibitors; D008689:Methacrylates; D010455:Peptides; D010756:Phosphoric Acids; D011109:Polymethacrylic Acids; D011140:Polyurethanes; D020011:Protective Agents; D019279:Resin Cements; D012464:Saliva, Artificial; D017640:Silicates; C485817:beta-tricalcium phosphate; C425330:bioactive glass 45S5; C052929:collagen type I trimeric cross-linked peptide; C029824:urethane dimethacrylate luting resin; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate; D003094:Collagen; D004492:Edetic Acid; C030242:phosphoric acid; D020782:Matrix Metalloproteinases; C031293:calcium silicate