Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue.
We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs, enabling routine whole transcriptome analysis of clinically valuable archival tissue specimens by Next-Generation Sequencing.
Genomic Health Inc., Redwood City, California, United States of America. jmorlan@genomichealth.com
John D
JD
Morlan
Kunbin
K
Qu
Dominick V
DV
Sinicropi
D018070:Biological Specimen Banks; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D016612:Paraffin Embedding; D012313:RNA; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D016707:Tissue Fixation
D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D012313:RNA