[Molecular epidemiology of viral diarrhea in Chengdu infants and young children].
OBJECTIVE
To investigated the molecular epidemiologic features of viral diarrhea in Chengdu infants and young children, and to establish baseline patterns of etiology, provides the scientific basis for the vaccine development and the epidemic situation control.


METHODS
From March, 2006 to December, 2008, a total of 376 infants and young children from Chengdu area hospitalized for diarrhea in Chengdu Children's Hospital were enrolled in this study. The stool specimen collected from each patient was tested for rotavirus (RV), Calicivirus (CV), astrovirus (AstV) and adenovirus (Adv) by using enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) examination.


RESULTS
Among those 376 cases,there were 142 cases (37.76%) of RV infections,which scattered predominantly in October to December. Among 234 cases RV negativity,there were 29 cases HuCV infections (15.85%), 5 cases AstV infections (1.64%), and 8 cases Adv infections (2.04%).


CONCLUSIONS
RV appeared to be the main etiological agent of viral diarrhea in Chengdu infants and young children,the predominant serotype of RV were G3, P[8] and P[4],HuCV might be the important etiological agent besides RV.
Children's hospital of Chengdu, Chengdu 610017, China.
Xao-Li
XL
Xie
Wei
W
Zhang
Xue-Chun
XC
Liao
Xiao-Zhi
XZ
Deng
Li-Rong
LR
Liu
Qi-Cheng
QC
Zhang
Li-Hong
LH
Shang
Yan
Y
Wen
Ming
M
Ren
D000260:Adenoviruses, Human / Q000235:genetics / Q000302:isolation & purification; D002139:Caliciviridae / Q000235:genetics / Q000302:isolation & purification; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D001257:Mamastrovirus / Q000235:genetics / Q000302:isolation & purification; D017720:Molecular Epidemiology; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification; D014777:Virus Diseases / Q000453:epidemiology* / Q000821:virology; D014780:Viruses / Q000235:genetics*