[Impact of Toxoplasma gondii on the proliferation and apoptosis of tumor cell lines].
MCF-7, DU-145, EC-109 and A549 tumor cells (5 x 10(4)nml) at mid-exponential phase were treated for 24h with different concentrations of Toxoplasma gondii tachyzoites (1 x 10(4)/ml-32x10(4)/ml). The measurement of tumor cell growth inhibition rate was performed by using CCK-8 kit. The tachyzoites (1 x 10(4)/ml-4 x 10(4)/ml) were co-cultured with A549 tumor cells for 24 h. Flow cytometry techniques was applied to investigate the expression of p53 and Bcl-2. The results showed a remarkable inhibitory effect of the parasites on tumor cell proliferation, which presented a dosage dependence. MCF-7, DU-145, EC-109 and A549 cell lines treated with 1 x 10(4) ml T. gondii tachyzoites presented an inhibition rate of 19.7%, 4.5%, 28.0%, and 20.8%, respectively. There was a significant difference between experimental groups and the control (P < 0.05). T. gondii induced cell apoptosis in A549 tumor cells, p53 and Bcl-2 gene expression was respectively up-regulated and down-regulated.
Department of Post-Graduates, Hebei North University, Zhangjiakou 075000, China.
Xin
X
Wu
Li
L
Sun
Li
L
Zhang
Zheng-Quan
ZQ
Liu
Qiang
Q
Luo
Lin-Xi
LX
Zhang
D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005434:Flow Cytometry; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D014122:Toxoplasma; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
D019253:Proto-Oncogene Proteins c-bcl-2; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53