Melanophore migration and survival during zebrafish adult pigment stripe development require the immunoglobulin superfamily adhesion molecule Igsf11.
The zebrafish adult pigment pattern has emerged as a useful model for understanding the development and evolution of adult form as well as pattern-forming mechanisms more generally. In this species, a series of horizontal melanophore stripes arises during the larval-to-adult transformation, but the genetic and cellular bases for stripe formation remain largely unknown. Here, we show that the seurat mutant phenotype, consisting of an irregular spotted pattern, arises from lesions in the gene encoding Immunoglobulin superfamily member 11 (Igsf11). We find that Igsf11 is expressed by melanophores and their precursors, and we demonstrate by cell transplantation and genetic rescue that igsf11 functions autonomously to this lineage in promoting adult stripe development. Further analyses of cell behaviors in vitro, in vivo, and in explant cultures ex vivo demonstrate that Igsf11 mediates adhesive interactions and that mutants for igsf11 exhibit defects in both the migration and survival of melanophores and their precursors. These findings identify the first in vivo requirements for igsf11 as well as the first instance of an immunoglobulin superfamily member functioning in pigment cell development and patterning. Our results provide new insights into adult pigment pattern morphogenesis and how cellular interactions mediate pattern formation.
Department of Biology, University of Washington, Seattle, Washington, United States of America.
Dae Seok
DS
Eom
Shinya
S
Inoue
Larissa B
LB
Patterson
Tiffany N
TN
Gordon
Rebecca
R
Slingwine
Shigeru
S
Kondo
Masakatsu
M
Watanabe
David M
DM
Parichy
D000818:Animals; D005075:Biological Evolution; D019521:Body Patterning / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000235:genetics*; D002454:Cell Differentiation; D002465:Cell Movement; D002470:Cell Survival; D004625:Embryo, Nonmammalian; D029941:Fish Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D007136:Immunoglobulins / Q000235:genetics*; D007814:Larva / Q000235:genetics; D008547:Melanophores / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D009154:Mutation; D010641:Phenotype; D010858:Pigmentation / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics*
D015815:Cell Adhesion Molecules; D029941:Fish Proteins; C576805:Igsf11 protein, zebrafish; D007136:Immunoglobulins; D029961:Zebrafish Proteins