Quantification of abscisic Acid, cytokinin, and auxin content in salt-stressed plant tissues.
Plant hormones cytokinins, auxin (indole-3-acetic acid), and abscisic acid are central to regulation of plant growth and defence to abiotic stresses such as salinity. Quantification of the hormone levels and determination of their ratios can reveal different plant strategies to cope with the stress, e.g., suppression of growth or mobilization of plant metabolism. This chapter describes a procedure enabling such quantification. Due to the high variability of these hormones in plant tissues, it is advantageous to determine their content in the same sample. Reverse phase and ion exchange chromatography allows separation of the individual hormone fractions. Hormones as well as their metabolites can be identified and quantified by LC/MS.
Institute of Experimental Botany AS CR, Prague, Czech Republic. dobrev@ueb.cas.cz
Petre I
PI
Dobrev
Radomira
R
Vankova
D000040:Abscisic Acid / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003583:Cytokinins / Q000032:analysis* / Q000302:isolation & purification; D007210:Indoleacetic Acids / Q000032:analysis* / Q000302:isolation & purification; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D010944:Plants / Q000737:chemistry* / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance / Q000502:physiology*; D013312:Stress, Physiological
D003583:Cytokinins; D007210:Indoleacetic Acids; D010936:Plant Extracts; D000040:Abscisic Acid