[In vitro anti-proliferation effect of peptides from cinobufacini injection].
Cinobufacini is an aqueous extract of Bufo bufo gargarizans Cantor dried skin, which has been widely used for cancer therapy in China. So far, its active components are still not very clear. In previous reports, bufadienolides with low-concentration were usually studied because of their anticancer effects. However, the high polarity constituents in cinobufacini are less investigated. The present study found that more than 50% contents of cinobufacini were water-soluble peptides. Then, in vitro anticancer experiments were carried out, including human stomach cancer cell lines BGC823 and MCG803, human colon cancer cell lines DLD-1 and HT-29, and human pancreatic cancer cell line MIAPACA-2. The IC50 for these cell lines model were ranged from 25-123 microgmL(-1). The results indicated that these peptides showed similar activity with cinobufacini injection. As a conclusion, this study provides a new and further understanding of anticancer components in cinobufacini injection.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Xu
X
Wu
Bo
B
Gao
Jian
J
Yang
Bao-Lin
BL
Bian
Hong-Jie
HJ
Wang
D000664:Amphibian Venoms / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002025:Bufonidae; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D019073:HT29 Cells; D006801:Humans; D007267:Injections; D008516:Medicine, Chinese Traditional; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D012867:Skin / Q000737:chemistry
D000664:Amphibian Venoms; D000970:Antineoplastic Agents; D010455:Peptides; C545813:huachansu