Nitric oxide and glutathione impact the expression of iron uptake- and iron transport-related genes as well as the content of metals in A. thaliana plants grown under iron deficiency.
Mounting evidence indicate that nitric oxide (NO) acts as a signaling molecule mediating iron deficiency responses through the upregulation of the expression of iron uptake-related genes. Accordingly, NO donors such as nitrosoglutathione (GSNO) were reported to improve the fitness of plants grown under iron deficiency. Here, we showed that glutathione, a by-product of GSNO, triggered the upregulation of the expression of iron uptake- and transport-related gene and an increase of iron concentration in Arabidopsis thaliana seedlings facing iron deficiency. Furthermore, we provided evidence that under iron deficiency, NO released by GSNO did not improve the root iron concentration but impacted the content of copper. Collectively, our data highlight the complexity of interpreting data based on the use of NO donors when investigating the role of NO in iron homeostasis.
Université de Bourgogne, UMR 1347 Agroécologie, Pôle Mécanisme et Gestion des Interactions Plantes-microorganismes, Dijon, France.
Emmanuel
E
Koen
Katarzyna
K
Szymańska
Agnès
A
Klinguer
Grażyna
G
Dobrowolska
Angélique
A
Besson-Bard
David
D
Wendehenne
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport / Q000235:genetics; D003300:Copper / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000235:genetics; D005978:Glutathione / Q000378:metabolism* / Q000494:pharmacology; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D000090463:Iron Deficiencies; D008345:Manganese / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D026422:S-Nitrosoglutathione / Q000494:pharmacology; D015032:Zinc / Q000378:metabolism
D029681:Arabidopsis Proteins; D009569:Nitric Oxide; D008345:Manganese; D026422:S-Nitrosoglutathione; D003300:Copper; D007501:Iron; D005978:Glutathione; D015032:Zinc