Dynamic compartmentalization of DNA repair proteins within spiral ganglion neurons in response to noise stress.
ABSTRACT  In response to stress, spiral ganglion neurons may remodel intracellular pools of DNA repair proteins. This hypothesis was addressed by determining the intracellular location of three classic DNA excision repair proteins (XPA, CSA, and XPC) within the neurons under normal conditions, one day after noise stress (105 dB/4 hr) and following DNA repair adjuvant therapy with carboxy alkyl esters (CAEs; 160 mg/kg/28 days). Under normal conditions, three intracellular compartments were enriched with at least one repair protein. These intracellular compartments were designated nuclear, cytoplasmic, and perinuclear. After the noise stress each repair protein aggregated in the cytoplasm. After CAE therapy each intracellular compartment was enriched with the three DNA repair proteins. Combining noise stress with CAE therapy resulted in the enrichment of at least two repair proteins in each intracellular compartment. The combined results suggest that in response to noise stress and/or otoprotective therapy, spiral ganglion neurons may selectively remodel compartmentalized DNA repair proteins.
Research Service-151, Loma Linda Veterans Affairs Medical Center, Loma Linda, California 92357, USA. O’neil.Guthrie@va.gov
O'neil W
OW
Guthrie
D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects / Q000502:physiology*; D045643:DNA Repair Enzymes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D004952:Esters / Q000627:therapeutic use; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D008297:Male; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D009622:Noise; D051381:Rats; D020318:Rats, Long-Evans; D013136:Spiral Ganglion / Q000473:pathology*; D013315:Stress, Psychological / Q000150:complications / Q000188:drug therapy / Q000473:pathology*
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D004952:Esters; C554673:XPA protein, rat; C534808:XPC protein, rat; D045643:DNA Repair Enzymes