Self-packed filter plates: a good alternative for pre-packed filter plates for developing purification processes for therapeutic proteins.
The use of high-throughput screening (HTS) has been successfully applied over the past years in downstream process development of therapeutic proteins. Different HTS applications have been introduced to speed up the development of purification processes for these proteins. In the light of these findings, studies were conducted to develop a controlled method of pipetting the right amount of resin into self-packed filter plates. In total, 13 plates were tested for their suitability in an HTS application. Based on these studies, the Seahorse plate was experimentally verified as the best filter plate. Suitable conditions for preparing a self-packed plate were established so that linear correlations between resin concentration (POROS 50HS) and UV adsorption (420 nm) were obtained. The accuracy of dispensing the slurry into a microtiter plate was controlled within 1% deviation under established conditions. Overall, self-packed filter plates are equipped to be used in the development of purification processes in HTS mode.
Synthon BV, Downstream Processing, Nijmegen, The Netherlands.
Xiaonan
X
Li
Guy
G
de Roo
Kim
K
Burgers
Marcel
M
Ottens
Michel
M
Eppink
D001391:Azo Compounds / Q000737:chemistry; D001709:Biotechnology / Q000295:instrumentation; D000431:Ethanol / Q000737:chemistry; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods; D007074:Immunoglobulin G / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*
D001391:Azo Compounds; D007074:Immunoglobulin G; D011506:Proteins; C008710:Orange G; D000431:Ethanol