A gas chromatography-mass spectrometry assay to quantify camphor extracted from goat serum.
A sensitive gas chromatography-mass spectrometry (GC-MS) method was developed and validated for quantification and pharmacokinetics of camphor, a major monoterpene of juniper plant, in goat serum. Camphor and internal standard (terpinolene) eluates from solid phase extraction (SPE) with ethyl acetate yielded well resolved peaks and were clearly identified in total and selected ion chromatograms. The elution and injection volumes were optimized for improved detection and quantification of camphor based on peak shape, signal to noise ratio, recoveries, and repeatability. The matrix calibration curve with the good linearity (R(2)=0.998) and response in the range of 0.005-10.0 μg/mL was used to determine camphor concentration in goat serum. The GC-MS method offered sufficiently low limits of detection (1 ng/mL) and quantitation (3 ng/mL) for camphor concentration in goat serum for the pharmacokinetic study. The proposed method showed good intra- and inter-day variation with relative standard deviation (RSD) of 0.2-7.7% and produced good recovery (96.0-111.6%) and reproducibility (1.6-6.1%) at all spiked levels. Using this method on serum samples obtained from two goats orally dosed with camphor confirmed that the method is suitable for camphor studies in animals.
Office of the Texas State Chemist, Texas AgriLife Research, Texas A&M University System, College Station, TX 77843, United States.
Kyung-Min
KM
Lee
Susie Y
SY
Dai
Timothy J
TJ
Herrman
Jeffrey M B
JM
Musser
D000284:Administration, Oral; D000818:Animals; D002164:Camphor / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006041:Goats / Q000097:blood*; D018653:Juniperus / Q000737:chemistry; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002164:Camphor