Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase onto alkylamine glass beads through glutaraldehyde coupling.
A method for co-immobilizing lipase from porcine pancreas, glycerol kinase (GK) from Cellulomonas spp., glycerol-3-phosphate oxidase (GPO) from Aerococcus viridans and peroxidase from horseradish onto zirconia-coated alkylamine glass beads through glutaraldehyde coupling has been described. The co-immobilized enzymes retained 71.4% of initial specific activity with a conjugation yield of 43.6 mg/g support. The optimum pH and Km for triolein increased, while Vmax was decreased slightly, but incubation temperature for maximum activity remained unaltered after co-immobilization. The co-immobilized enzymes showed increased thermal and storage stabilities in cold, compared to their native form. Among the various metal salts tested, only CuSO4 caused inhibition of both free and co-immobilized enzymes. The co-immobilized enzymes showed better suitability over mixture of individually immobilized enzymes in determination of serum triglyceride.
Biochemistry Research Laboratory, Dept of Bio-Sciences, M.D. University, Rohtak 124 001 (Haryana), India.
Vandana
V
Kalia
C S
CS
Pundir
D056570:Aerococcus / Q000201:enzymology; D000818:Animals; D031215:Armoracia; D004800:Enzymes, Immobilized; D005898:Glass; D005976:Glutaral / Q000737:chemistry*; D005991:Glycerol Kinase / Q000737:chemistry* / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000737:chemistry* / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000737:chemistry; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry* / Q000378:metabolism; D013552:Swine; D013696:Temperature; D013997:Time Factors; D014280:Triglycerides / Q000097:blood; D015040:Zirconium / Q000737:chemistry
D004800:Enzymes, Immobilized; D014280:Triglycerides; D015040:Zirconium; D005993:Glycerolphosphate Dehydrogenase; C098831:glycerol-3-phosphate oxidase; D006735:Horseradish Peroxidase; D009195:Peroxidase; D005991:Glycerol Kinase; D008049:Lipase; D005976:Glutaral