Differential expression of miR-21, miR-125b and miR-191 in breast cancer tissue.
OBJECTIVE
To develop new biomarkers for early detection and to inform effective clinical management of breast cancer.


METHODS
Real-time polymerase chain reaction was used to profile microRNA (miRNA) in tumor tissue from 50 breast cancer patients using non-tumor breast tissue from each patient as a control. We have focussed on three miRNA; miR-21, miR-125b and miR-191, all of which have been implicated in breast cancer with either proven or predicted target genes involved in critical cancer-associated cellular pathways.


RESULTS
Upregulation of miR-21 and miR-191 and downregulation of miR-125b, was found in breast cancer tissue. Combined expression analysis of miR-125b/miR-191 increased sensitivity to 100% and specificity to 94% while miR-21/miR-191 increased to 92% and 100%, respectively. Therefore, combination of two miRNA gives a better prediction than individual miRNA.


CONCLUSIONS
We could differentiate between breast cancer and adjacent non-tumor breast tissue as a control with a high degree of sensitivity and specificity in the Mexican population using a combined expression analysis of only two miRNA. These observations, although a proof of principle finding at this time, show that a combined expression profile of two miRNA (miR-125b/miR-191 and miR-21/miR-191) can discriminate between breast cancer and non-tumor tissue with high specificity and sensitivity.
Department of Cellular Biology and Genetics, College of Biological Sciences, Autonomous University of Nuevo Leon, San Nicolás de los Garza, Nuevo León, Mexico.
Fermín
F
Mar-Aguilar
Claudia M
CM
Luna-Aguirre
J Claudio
JC
Moreno-Rocha
Juan
J
Araiza-Chávez
Victor
V
Trevino
Cristina
C
Rodríguez-Padilla
Diana
D
Reséndez-Pérez
D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D001940:Breast / Q000378:metabolism* / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000473:pathology; D018275:Carcinoma, Lobular / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; C531888:MIRN125 microRNA, human; C546177:MIRN191 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger