Functional heterogeneity of the UpaH autotransporter protein from uropathogenic Escherichia coli.
Uropathogenic Escherichia coli (UPEC) is responsible for the majority of urinary tract infections (UTI). To cause a UTI, UPEC must adhere to the epithelial cells of the urinary tract and overcome the shear flow forces of urine. This function is mediated primarily by fimbrial adhesins, which mediate specific attachment to host cell receptors. Another group of adhesins that contributes to UPEC-mediated UTI is autotransporter (AT) proteins. AT proteins possess a range of virulence properties, such as adherence, aggregation, invasion, and biofilm formation. One recently characterized AT protein of UPEC is UpaH, a large adhesin-involved-in-diffuse-adherence (AIDA-I)-type AT protein that contributes to biofilm formation and bladder colonization. In this study we characterized a series of naturally occurring variants of UpaH. We demonstrate that extensive sequence variation exists within the passenger-encoding domain of UpaH variants from different UPEC strains. This sequence variation is associated with functional heterogeneity with respect to the ability of UpaH to mediate biofilm formation. In contrast, all of the UpaH variants examined retained a conserved ability to mediate binding to extracellular matrix (ECM) proteins. Bioinformatic analysis of the UpaH passenger domain identified a conserved region (UpaH(CR)) and a hydrophobic region (UpaH(HR)). Deletion of these domains reduced biofilm formation but not the binding to ECM proteins. Despite variation in the upaH sequence, the transcription of upaH was repressed by a conserved mechanism involving the global regulator H-NS, and mutation of the hns gene relieved this repression. Overall, our findings shed new light on the regulation and functions of the UpaH AT protein.
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia.
Luke P
LP
Allsopp
Christophe
C
Beloin
Danilo Gomes
DG
Moriel
Makrina
M
Totsika
Jean-Marc
JM
Ghigo
Mark A
MA
Schembri
D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development; D019295:Computational Biology; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D014644:Genetic Variation; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D056689:Uropathogenic Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism*
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D016326:Extracellular Matrix Proteins; D050505:Mutant Proteins; C548680:UpaH protein, E coli; D037521:Virulence Factors