Identification of serotonin-sensitive aryl acylamidase activity with cobra venom acetylcholinesterase.
Acetylcholinesterase purified from cobra (Naja naja) venom exhibits a serotonin-sensitive aryl acylamidase activity. Both acetylcholinesterase and aryl acylamidase activities co-eluted in column chromatographic procedures (Sephadex G-75 and Zinc-Sepharose), co-migrated on polyacrylamide gel electrophoresis, co-immunoprecipitated by anti-snake venom antibody and showed the same heat denaturation profile at 40 degrees C. Further, several potent acetylcholinesterase inhibitors at different concentrations inhibited the cholinesterase and aryl acylamidase activities to the same extent. It is concluded that in cobra venom, acetylcholinesterase is associated with a serotonin-sensitive aryl acylamidase activity similar to earlier observations made with acetylcholinesterase from different sources.
Department of Biotechnology, Enzyme Biotech Lab, Bharathiar University, Coimbatore 641 046, India.
Ramanna V
RV
Rajesh
Aiylam S
AS
Balasubramanian
Rathanam
R
Boopathy
D000110:Acetylcholinesterase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000581:Amidohydrolases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D017838:Bungarus; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004546:Elapid Venoms / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D006358:Hot Temperature; D007531:Isoflurophate / Q000494:pharmacology; D011489:Protein Denaturation / Q000187:drug effects; D012701:Serotonin / Q000378:metabolism*; D018490:Serotonin Agents / Q000494:pharmacology
D002800:Cholinesterase Inhibitors; D004546:Elapid Venoms; D018490:Serotonin Agents; D007531:Isoflurophate; D012701:Serotonin; D000110:Acetylcholinesterase; D000581:Amidohydrolases; C019732:aryl acylamidase