Ischaemic postconditioning rescues brain injury caused by focal ischaemia/reperfusion via attenuation of protein oxidization.
OBJECTIVE
To investigate the effects of ischaemic postconditioning on brain injury and protein oxidization in focal ischaemia/reperfusion.


METHODS
Adult male Wistar rats (n = 30) were randomly divided into sham-operated, ischaemia, and ischaemic postconditioning groups. Ischaemia  was produced by middle cerebral artery occlusion and ischaemic postconditioning was performed using three cycles of 30-s/30-s reperfusion/reocclusion after 2 h of ischaemia. Brain infarction size, hydrogen peroxide concentration, superoxide dismutase (SOD), catalase (CAT) and proteasome activities,  protein carbonyl derivatives and advanced oxidized protein products (AOPPs) were evaluated.


RESULTS
The size of brain infarction after ischaemic postconditioning was significantly smaller compared with the ischaemia group, and was concomitant with significant reduction in protein carbonyl  derivatives and AOPPs. The activities of SOD, CAT and proteasomes were elevated by ischaemic postconditioning compared with the ischaemia group.


CONCLUSIONS
Ischaemic postconditioning is an effective way of reducing the size and effects of brain infarction caused by focal ischaemia/reperfusion,  possibly due to a decrease in oxidized protein levels. Decreasing protein oxidization may, therefore, be a useful target for preventing cerebral injury.
Department of Neurosurgery, First Bethune Hospital of Jilin University, Changchun, China.
Z Y
ZY
Li
B
B
Liu
J
J
Yu
F W
FW
Yang
Y N
YN
Luo
P F
PF
Ge
D000818:Animals; D001930:Brain Injuries / Q000201:enzymology / Q000378:metabolism / Q000517:prevention & control*; D002545:Brain Ischemia / Q000201:enzymology / Q000378:metabolism* / Q000601:surgery; D002374:Catalase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D019194:Ischemic Preconditioning; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D009419:Nerve Tissue Proteins; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase