Capsaicin induces de-differentiation of activated hepatic stellate cell.
Hepatic stellate cells (HSC) play a key role in liver fibrogenesis. Activation of PPARγ and inhibition of fibrogenic molecules are potential strategies to block HSC activation and differentiation. A number of natural products have been suggested to have antifibrotic effects for the de-activation and de-differentiation of HSCs. The purpose of this study was to investigate the in vitro effects of capsaicin on HSC de-activation and de-differentiation. The results demonstrated that capsaicin induced quiescent phenotype in GRX via PPARγ activation. Significant decrease in COX-2 and type I collagen mRNA expression was observed in the first 24 h of treatment. These events preceded the reduction of TGF-β1 and total collagen secretion. Thus, capsaicin promoted down-regulation of HSC activation by its antifibrotic and anti-inflammatory actions. These findings demonstrate that capsaicin may have potential as a novel therapeutic agent for the treatment of liver fibrosis.
Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul, PUCRS, Avenida Ipiranga 6681, prédio 12, bloco C, sala 221, CEP 90619-900, Porto Alegre, Rio Grande do Sul, Brazil.
Shanna
S
Bitencourt
Fernanda C
FC
de Mesquita
Eduardo
E
Caberlon
Gabriela V
GV
da Silva
Bruno S
BS
Basso
Gabriela A
GA
Ferreira
Jarbas R
JR
de Oliveira
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D055166:Hepatic Stellate Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D047495:PPAR gamma; D053773:Transforming Growth Factor beta1; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D002211:Capsaicin