Trophoblastic cell lines ACH1P and AC-1M32 react with a distinctive cytokine pattern toward Listeria monocytogenes and show morphologic differences.
OBJECTIVE
The differential reaction of cytotrophoblast and syncytiotrophoblast towards infection with listeria monocytogenes (LM) is pivotal to its pathogenicity. In this study we tested the cytokine signature upon infection with listeria monocytogenes (LM) in an in vitro model.


METHODS
We compared two related trophoblastic cell lines (AC-1M32 and ACH1P). The cell line ACH1P showed syncytium formation, whereas AC-1M32 did not fuse, as demonstrated with immunfluorescence E-Cadherin staining. In a Multi-Analyte ELISArray we tested for concentrations of TNFα, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IL-13, IL-17A, IL-8, MCP1, MIP-1a, MIP-1b, MDC, Eotaxin, IFNγ, G-CSF, TGFβ1 after 8 and 24 hours.


RESULTS
Compared to unstimulated cells, the syncytial cell line ACH1P showed a significant induction of Interleukin-6 (IL-6), Monocyte Chemotactic Protein-1 (MCP-1) and transforming growth factor β-1 (TGFβ1). Incubating AC-1M32 with LM, however, showed significantly reduced IL-6 levels and a massively increased (~300fold) TGFβ1 secretion compared to unstimulated controls.


CONCLUSIONS
A functional anti-LM immune response was only induced by the syncytium-forming cell line ACH1P. Using the two sister cell lines AC-1M32 and ACH1P in an in vitro LM-stimulation assay might facilitate research in the area of placental listeria infection.
Department of Obstetrics and Gynecology, Ludwig-Maximilians University, Munich, Germany.
Laura
L
Santoso
Klaus
K
Friese
Udo
U
Jeschke
Christoph
C
Scholz
D002460:Cell Line; D018932:Chemokine CCL2 / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D015726:Giant Cells / Q000648:ultrastructure*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008089:Listeria monocytogenes / Q000276:immunology / Q000472:pathogenicity*; D008088:Listeriosis / Q000276:immunology* / Q000382:microbiology; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014327:Trophoblasts / Q000276:immunology* / Q000382:microbiology* / Q000648:ultrastructure
D018932:Chemokine CCL2; D016207:Cytokines; D015850:Interleukin-6; D016212:Transforming Growth Factor beta