In vitro evaluation of antiprotozoal and antiviral activities of extracts from Argentinean Mikania species.
The aim of this study was to investigate the antiprotozoal and antiviral activities of four Argentinean Mikania species. The organic and aqueous extracts of Mikania micrantha, M. parodii, M. periplocifolia, and M. cordifolia were tested on Trypanosoma cruzi epimastigotes, Leishmania braziliensis promastigotes, and dengue virus type 2. The organic extract of M. micrantha was the most active against T. cruzi and L. braziliensis exhibiting a growth inhibition of 77.6 ± 4.5% and 84.9 ± 6.1%, respectively, at a concentration of 10 μg/ml. The bioguided fractionation of M. micrantha organic extract led to the identification of two active fractions. The chromatographic profile and infrared analysis of these fractions revealed the presence of sesquiterpene lactones. None of the tested extracts were active against dengue virus type 2.
Cátedra de Farmacognosia, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 2°P, 1113, Buenos Aires, Argentina.
Laura C
LC
Laurella
Fernanda M
FM
Frank
Andrea
A
Sarquiz
María R
MR
Alonso
Gustavo
G
Giberti
Lucia
L
Cavallaro
Cesar A
CA
Catalán
Silvia I
SI
Cazorla
Emilio
E
Malchiodi
Virginia S
VS
Martino
Valeria P
VP
Sülsen
D000981:Antiprotozoal Agents / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003716:Dengue Virus / Q000187:drug effects; D066298:In Vitro Techniques; D007892:Leishmania braziliensis / Q000187:drug effects; D035742:Mikania / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D014349:Trypanosoma cruzi / Q000187:drug effects
D000981:Antiprotozoal Agents; D000998:Antiviral Agents; D010936:Plant Extracts