Evaluation of oleoresin capsicum of Capsicum frutescenes var. Nagahari containing various percentages of capsaicinoids following inhalation as an active ingredient for tear gas munitions.
Comparative efficacy as peripheral sensory irritant, oral and inhalation exposure studies were carried out on oleoresin capsicum (OC) of Capsicum frutescence var. Nagahari containing various percentages of capsaicinoids and two synthetic isomers of capsaicin in Swiss albino male mouse model to come up with a suitable active ingredient from natural source for tear gas munitions. The compounds screened were OC having varying percentages of capsaicinoids (20, 40 and 80%, respectively) and synthetic isomers (E and Z) of capsaicin (8-methyl-N-vanillyl-6-nonenamide). Mice were exposed to pyrotechnically generated smoke of the compounds in an all glass static exposure chamber for 15 min to determine acute inhalation toxicity (LC₅₀) and quantitative sensory irritation potential (RD₅₀). Acute oral median lethal dose (LD₅₀) was also evaluated. Safety index of tear gas (SITG), a ratio of lethal concentration 50% (LC₅₀) and the concentration which depresses respiration by 50% (RD₅₀) due to peripheral sensory irritation is also proposed. The compound having highest SITG is considered as the most suitable to be used for tear gas munitions. The study revealed that oleoresin capsicum containing 40% capsaicinoids had the highest SITG among the compounds studied. The oral dosage versus mortality pattern of some compounds did not follow a true dose-response curve (DRC); however, following inhalation, all the compounds followed DRC. It was concluded that oleoresin capsicum (40% capsaicinoids) may be considered as the most suitable and environmental friendly compound from natural source to be used as an active ingredient for tear gas munitions.
Defence Research and Development Establishment, Gwalior-474002, India. pravinkumar43@hotmail.com
Pravin
P
Kumar
Utsab
U
Deb
M P
MP
Kaushik
D000284:Administration, Oral; D000465:Algorithms; D000818:Animals; D001273:Atmosphere Exposure Chambers; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000633:toxicity; D002212:Capsicum / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004787:Environmental Pollution / Q000517:prevention & control; D005638:Fruit / Q000737:chemistry*; D019570:Inhalation Exposure / Q000009:adverse effects*; D007509:Irritants / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity*; D012119:Respiration / Q000187:drug effects; D012131:Respiratory Insufficiency / Q000139:chemically induced; D012906:Smoke; D013237:Stereoisomerism; D013665:Tear Gases / Q000737:chemistry / Q000633:toxicity*
D007509:Irritants; D010936:Plant Extracts; D012906:Smoke; D013665:Tear Gases; C018620:oleoresins; D002211:Capsaicin