Bacteriophage vB_EcoM_FV3: a new member of "rV5-like viruses".
A proposed new genus of the family Myoviridae, "rV5-like viruses", includes two lytic bacteriophages: Escherichia coli O157: H7-specific bacteriophage rV5 and Salmonella phage PVP-SE1. Here, we present basic properties and genomic characterization of a novel rV5-like phage, vB_EcoM_FV3, which infects E. coli K-12-derived laboratory strains and replicates at high temperature (up to 47 °C). The 136,947-bp genome of vB_EcoM_FV3 contains 218 open reading frames and encodes 5 tRNAs. The genomic content and organization of vB_EcoM_FV3 is more similar to that of rV5 than to PVP-SE1, but all three phages share similar morphological characteristics and form a homogeneous phage group.
Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry of Vilnius University, Mokslininkų 12, 08662 Vilnius, Lithuania. lidija.truncaite@bchi.vu.lt
Lidija
L
Truncaite
Eugenijus
E
Šimoliūnas
Aurelija
A
Zajančkauskaite
Laura
L
Kaliniene
Roma
R
Mankevičiūte
Juozas
J
Staniulis
Vytautas
V
Klausa
Rolandas
R
Meškys
D001422:Bacterial Adhesion; D003080:Cold Temperature; D004279:DNA, Viral / Q000235:genetics; D048168:Escherichia coli K12 / Q000145:classification / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000145:classification* / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D014779:Virus Replication
D004279:DNA, Viral; D012329:RNA, Bacterial; D012343:RNA, Transfer