A fermentation-powered thermopneumatic pump for biomedical applications.
We present a microorganism-powered thermopneumatic pump that utilizes temperature-dependent slow-kinetics gas (carbon dioxide) generating fermentation of yeast as a pressure source. The pump consists of stacked layers of polydimethylsiloxane (PDMS) and a silicon substrate that form a drug reservoir, and a yeast-solution-filled working chamber. The pump operates by the displacement of a drug due to the generation of gas produced via yeast fermentation carried out at skin temperatures. The robustness of yeast allows for long shelf life under extreme environmental conditions (50 °C, >250 MPa, 5-8% humidity). The generation of carbon dioxide is a linear function of time for a given temperature, thus allowing for a controlled volume displacement. A polymeric prototype (dimensions 15 mm × 15 mm × 10 mm) with a slow flow rate of <0.23 μL min(-1) and maximum backpressure of 5.86 kPa capable of continuously pumping for over two hours is presented and characterized.
School of Electrical and Computer Engineering, Purdue University, West Lafayette, IN 47907, USA.
Manuel
M
Ochoa
Babak
B
Ziaie
D002245:Carbon Dioxide / Q000378:metabolism; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D005285:Fermentation; D006801:Humans; D007260:Infusion Pumps; D008845:Microinjections / Q000295:instrumentation* / Q000379:methods*; D009757:Nylons / Q000737:chemistry; D011312:Pressure; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D012881:Skin Temperature; D013997:Time Factors
D004129:Dimethylpolysiloxanes; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer; D002245:Carbon Dioxide