Effect of salt stress on genes encoding translation-associated proteins in Arabidopsis thaliana.
Salinity negatively affects plant growth and disturbs chloroplast integrity. Here, we aimed at identifying salt-responsive translation-related genes in Arabidopsis thaliana with an emphasis on those encoding plastid-located proteins. We used quantitative real-time PCR to test the expression of 170 genes after short-term salt stress (up to 24 h) and identified several genes affected by the stress including: PRPL11, encoding plastid ribosomal protein L11, ATAB2, encoding a chloroplast-located RNA-binding protein presumably functioning as an activator of translation, and PDF1B, encoding a peptide deformylase involved in N-formyl group removal from nascent proteins synthesized in chloroplasts. These genes were previously shown to have important functions in chloroplast biology and may therefore represent new targets for biotechnological optimization of salinity tolerance.
University of Potsdam; Institute of Biochemistry and Biology; Potsdam, Germany.
Mohammad Amin
MA
Omidbakhshfard
Nooshin
N
Omranian
Farajollah Shahriari
FS
Ahmadi
Zoran
Z
Nikoloski
Bernd
B
Mueller-Roeber
D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D060365:Chloroplast Proteins / Q000235:genetics / Q000378:metabolism; D002736:Chloroplasts / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000187:drug effects*; D014176:Protein Biosynthesis / Q000187:drug effects / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D055049:Salt Tolerance / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000235:genetics*
D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; D012965:Sodium Chloride