Nanosized copper oxide induces apoptosis through oxidative stress in podocytes.
Nanosized copper oxide (nano-CuO) has been widely used in many fields. Recent studies have shown that nano-CuO has toxic effects on various organs, but the effects of nano-CuO on kidney remain unclear. The aim of this study was to assess whether nano-CuO can induce the apoptosis of podocytes. The result of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay indicated that nano-CuO decreased podocyte viability in a concentration-dependent and time-dependent manner. The apoptotic assay by flow cytometry showed that nano-CuO induced podocyte apoptosis significantly. Meanwhile, the result of flow cytometric assay gave a clear indication that nano-CuO increased reactive oxygen species (ROS) level. The measurement of superoxide dismutase (SOD) and malondialdehyde (MDA) showed that nano-CuO decreased SOD and increased MDA levels in podocytes. These results may provide basic information for the safe application of nano-CuO in the future.
College of Medicine, Nankai University, Tianjin 300071, China.
Jing
J
Xu
Zhigui
Z
Li
Pengjuan
P
Xu
Lu
L
Xiao
Zhuo
Z
Yang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D008315:Malondialdehyde / Q000378:metabolism; D053768:Metal Nanoparticles / Q000633:toxicity*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D050199:Podocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D003300:Copper; D013482:Superoxide Dismutase; C030973:cupric oxide