Some contributions to the application of LC-NMR, LC-MS, and LC-CD to the biosynthesis of isoquinoline alkaloids using callus cultures.
Hyphenated spectroscopic techniques in combination with a special extraction and work-up of plant calli cultures of Berberidaceae, Fumariaceae, and Papaveraceae families, e.g., enabled us to get deeper insight into the sequential biochemical conversions of precursors into simple isoquinoline- and protoberberine-alkaloids and their follow-up-products with different skeletons. Some new alkaloids of these types have been found.
Department of Natural Medicinal Chemistry, Kobe Pharmaceutical University, Japan. kinuko@mx5.canvas.ne.jp
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K
Iwasa
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Moriyasu
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W
Wiegrebe
D000470:Alkaloids / Q000032:analysis / Q000096:biosynthesis*; D028141:Berberidaceae / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002942:Circular Dichroism / Q000379:methods*; D031310:Fumarioideae / Q000737:chemistry; D007546:Isoquinolines / Q000032:analysis / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D028101:Papaveraceae / Q000737:chemistry; D010944:Plants / Q000737:chemistry*; D029626:Ranunculaceae / Q000737:chemistry
D000470:Alkaloids; D007546:Isoquinolines