Expression pattern of adipocyte fatty acid-binding protein gene in different tissues and its regulation of genes related to adipocyte differentiation in duck.
Abundance of duck adipocyte fatty acid-binding protein (A-FABP) mRNA was detected in this study by quantitative real-time PCR. The results showed that duck A-FABP gene was expressed in muscular tissues and many organs, except pancreas, lung, and kidney, and highly expressed in adipose tissues, especially in sebum. The expression of A-FABP and adipocyte differentiation-related genes was upregulated by oleic acid, and the A-FABP knockdown suppressed the oleic acid-stimulated expression of these genes in the cultured duck adipocytes, indicating A-FABP might play an important role in duck adipocyte differentiation.
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Hangzhou, Zhejiang 310021, China.
J
J
He
Y
Y
Tian
J J
JJ
Li
J D
JD
Shen
Z R
ZR
Tao
Y
Y
Fu
D
D
Niu
L Z
LZ
Lu
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D004372:Ducks / Q000502:physiology*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D008297:Male; D019301:Oleic Acid; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; D019301:Oleic Acid