Fine bubble mixing (FBM) culture of E. coli: a highly cost-effective middle scale-size culture system.
A highly cost-effective and easy-to-assemble cultivation system suitable for middle scale-size culturing of bacterial cells is described. In the culture, from a flat-shaped air-stone with large surface area, fine bubbles are generated with a low-cost air pump available in an aquarium fish shop, and cell-agitation and oxygen supply are efficiently conducted by fine bubbles simultaneously. Growth properties of the cells and their saturation density are comparable to those in a conventional culture system. The expression of recombinant protein was revealed to be similar to conventional methods. The system does not require any expensive machines or equipments. In addition, all equipments except plastic flat-shaped airstone are reusable after sterilization. Due to the low cost, the ease to use and multiple cultivations at once, our system may enable to find better culture conditions, to scale-up with ease and to perform timesaving efficient protein production.
Expert Laboratory for Life Environments-ELLE, Department of Genome System Sciences, Graduate School of Nanobiosciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan. hyasumit@yokohama-cu.ac.jp
Hidetaro
H
Yasumitsu
Hitoshi
H
Tajima
Masaharu
M
Isobe
Sinsuke
S
Kutsuna
Sarkar M A
SM
Kawsar
Yuki
Y
Fujii
Robert A
RA
Kanaly
Yasuhiro
Y
Ozeki
Eriko
E
Yokota
D018929:Cell Culture Techniques / Q000191:economics / Q000379:methods*; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins