Fluorescence suppression using wavelength modulated Raman spectroscopy in fiber-probe-based tissue analysis.
In the field of biomedical optics, Raman spectroscopy is a powerful tool for probing the chemical composition of biological samples. In particular, fiber Raman probes play a crucial role for in vivo and ex vivo tissue analysis. However, the high-fluorescence background typically contributed by the auto fluorescence from both a tissue sample and the fiber-probe interferes strongly with the relatively weak Raman signal. Here we demonstrate the implementation of wavelength-modulated Raman spectroscopy (WMRS) to suppress the fluorescence background while analyzing tissues using fiber Raman probes. We have observed a significant signal-to-noise ratio enhancement in the Raman bands of bone tissue, which have a relatively high fluorescence background. Implementation of WMRS in fiber-probe-based bone tissue study yielded usable Raman spectra in a relatively short acquisition time (∼30  s), notably without any special sample preparation stage. Finally, we have validated its capability to suppress fluorescence on other tissue samples such as adipose tissue derived from four different species.
University of St Andrews, SUPA, School of Physics & Astronomy, North Haugh, St Andrews, Fife, Scotland, KY16 9SS, United Kingdom. bb295@st-andrews.ac.uk
Bavishna B
BB
Praveen
Praveen C
PC
Ashok
Michael
M
Mazilu
Andrew
A
Riches
Simon
S
Herrington
Kishan
K
Dholakia
D000465:Algorithms; D000818:Animals; D016477:Artifacts; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D002645:Chickens; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D005453:Fluorescence; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods*; D013552:Swine; D014159:Transducers