Morphological and chemical changes in dentin after using endodontic agents: fourier transform Raman spectroscopy, energy-dispersive x-ray fluorescence spectrometry, and scanning electron microscopy study.
We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposing the pulp chamber and divided by six groups (n=5): NT-no treatment; CHX-2% chlorhexidine; CHXE-2% chlorhexidine+17% EDTA; E-17% EDTA; SH5-5.25% NaOCl; SH5E-5.25% NaOCl+17% EDTA. The inorganic and organic content was analyzed by FT-Raman. μEDXRF examined calcium (Ca) and phosphorus (P) content as well as Ca/P ratio. Impressions of specimens were evaluated by SEM. Data were submitted to Kruskal-Wallis and Dunn tests (p<0.05). Differences were observed among groups for the 960 cm(-1) peak. Ca and P content differences were significant (SH5>NT=SH5E>CHX>E>CHXE). CHXE and E presented the highest Ca/P ratio values compared to the other groups (p<0.05). The SEM images in the EDTA-treated groups had the highest number of open tubules. Erosion in the tubules was observed in CHX and SH5E groups. Endodontic agents change the inorganic and organic content of pulp chamber dentin. NaOCl used alone, or in association with EDTA, was the most effective agent considering chemical and morphological approaches.
University of Campinas, Pediatric Dentistry Department, Piracicaba Dental School, Av. Limeira, 901, Piracicaba, SP 13414-903, Brazil. fmpascon@fop.unicamp.br
Fernanda Miori
FM
Pascon
Kamila Rosamilia
KR
Kantovitz
Luís Eduardo Silva
LE
Soares
Ana Maria do Espírito
AM
Santo
Airton Abrahão
AA
Martin
Regina Maria
RM
Puppin-Rontani
D003804:Dentin / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D008968:Molecular Conformation / Q000528:radiation effects; D009067:Mouthwashes / Q000494:pharmacology*; D012388:Root Canal Irrigants / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared
D009067:Mouthwashes; D012388:Root Canal Irrigants