Effect of flavan-3-ols on the adhesion of potential probiotic lactobacilli to intestinal cells.
The effect of dietary flavan-3-ols on the adhesion of potential probiotic lactobacilli strains to intestinal cells was unraveled. The inhibitory activity of these compounds on intestinal cells was highlighted. The cytotoxic effect was shown to depend on both the compound's chemical structure (galloylation and polymerization) and degree of differentiation of intestinal cells. The effect of flavan-3-ols on bacteria adhesion differed greatly between compounds, strains, and intestinal cells. All flavan-3-ols inhibited significantly  Lactobacillus acidophilus  LA-5 and  Lactobacillus plantarum  IFPL379 adhesion except epigallocatechin gallate, which enhanced L. acidophilus LA-5 adhesion to Caco-2. Procyanidins B1 and B2 increased remarkably the adhesion of  Lactobacillus casei  LC115 to HT-29 cells, whereas epigallocatechin increased L. casei LC115 adhesion to Caco-2. These data showed the potential of flavan-3-ols to alter gut microecology by modifying adhesion of lactobacilli strains to intestinal cells.
Department of Biotechnology and Microbiology, Instituto de Investigación en Ciencias de la Alimentación, CIAL ( CSIC-UAM ), CEI UAM+CSIC, Nicolás Cabrera 9, 28049 Madrid, Spain.
Irene
I
Bustos
Tomás
T
García-Cayuela
Blanca
B
Hernández-Ledesma
Carmen
C
Peláez
Teresa
T
Requena
M Carmen
MC
Martínez-Cuesta
D001422:Bacterial Adhesion / Q000187:drug effects*; D018938:Caco-2 Cells; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007778:Lactobacillus / Q000187:drug effects* / Q000502:physiology*; D019936:Probiotics / Q000378:metabolism
D005419:Flavonoids; C404987:flavan-3-ol