Very-long-distance correlations in proteins revealed by solid-state NMR spectroscopy.
I still see you: a new pulse sequence, SHA+, little sensitive to dipolar truncation, allows direct or relayed polarization transfer between (13)C atoms, distant by 3.5-9.6 Å, in amyloid fibrils. SHA+ can also be used in a broadband way with the weak rf-condition of ν(1)/ν(R)≈0.2-0.3 which permits the investigation of temperature-sensitive biological systems.
Physics Department, Shanghai Key Laboratory of Magnetic Resonance, East China Normal University, China. bwhu@phy.ecnu.edu.cn
Bingwen
B
Hu
Julien
J
Trébosc
Oliver
O
Lafon
Qun
Q
Chen
Yuichi
Y
Masuda
K
K
Takegoshi
Jean-Paul
JP
Amoureux
D016229:Amyloid beta-Peptides / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation; D013696:Temperature
D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)