An ABC transporter complex containing S-adenosylmethionine (SAM)-induced ATP-binding protein is involved in antibiotics production and SAM signaling in Streptomyces coelicolor M145.
A sco3956-deletion mutant (ΔSCO3956) of Streptomyces coelicolor was generated to characterize the S-adenosylmethionine (SAM)-induced, ATP-binding cassette transporter (ABC transporter) ATP-binding protein, SCO3956. It produced actinorhodin (ACT) and undecylprodigiosin (RED) decreased by approx. 82 and 64 %, respectively. In addition, the effect of exogenous SAM was lost in the ΔSCO3956. Plasmid-based complementation of sco3956 in ΔSCO3956 restored ACT and RED levels of ΔSCO3956 to wild-type levels (ACT: 20 ± 1.4 mg g(-1) DCW and RED: 5.3 ± 0.6 mg g(-1) DCW) and the exogenous effect significantly increased ACT and RED by approx. 129 and 135 %, respectively, when compared to the exogenous SAM non-treated sco3956 complementation strain. Thus, the ABC transporter ATP-binding protein, SCO3956, plays a critical role in ACT and RED production serving as a transducer of SAM signaling.
Division of Bioscience and Bioinformatics, Myongji University, Yongin 449-728, Korea.
Sung-Kwon
SK
Lee
Sangjoon
S
Mo
Joo-Won
JW
Suh
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism*; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D017384:Sequence Deletion; D015398:Signal Transduction; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism; D000095622:Benzoisochromanequinones
D018528:ATP-Binding Cassette Transporters; D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D011353:Prodigiosin; D012436:S-Adenosylmethionine; C047347:undecylprodigiosin; C013390:actinorhodin; D000095622:Benzoisochromanequinones