Chemical composition and antimicrobial activity of essential oil of Heracleum rigens.
The essential oil was extracted from the seeds of Heracleum rigens by hydrodistillation and a total of twenty compounds accounting for 98.5% of the total oil composition were identified. Physicochemical properties and chemical composition of the oil was determined by a combination GC/FID and GC/MS analysis. The major compounds identified were bornyl acetate (51.2%), alpha-pinene (22.6%), limonene (9.62%), octyl acetate (3.94%), rho-cymene (2.85%) and gamma-terpinene (1.93%). The antimicrobial activity of the oil was screened by the disc diffusion method against nine pathogenic bacterial strains. Maximum antimicrobial activity was noted against Klebsiella pneumonia and Bacillus subtillis. This investigation corroborates the traditional claim of H. rigens as an effective antimicrobial agent.
Department of P. G Studies and Research in Biotechnology, Government Science College, Bangalore 560 001, India.
Nataraj
N
Jagannath
Hanumanthaiah
H
Ramakrishnaiah
Venkatarangaiah
V
Krishna
Prameela Javarayi
PJ
Gowda
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D000081008:Bicyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D030002:Heracleum / Q000737:chemistry*; D007711:Klebsiella pneumoniae / Q000187:drug effects; D000077222:Limonene; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D013729:Terpenes / Q000737:chemistry
D000900:Anti-Bacterial Agents; D000081008:Bicyclic Monoterpenes; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D013729:Terpenes; C018669:gamma-terpinene; D000077222:Limonene; C005451:alpha-pinene