[Optimization of the codon strengthened the human rotavirus VP6 antigen's serum immune responses and protective responses in mice model].
OBJECTIVE
To observe the serum immune responses and protection in mice model of the recombinant adenovirus vector mediated human rotavirus VP6 gene expression through coden optimization (rvAdVP6(o)) in comparison with the wild type (rvAdVP6).


METHODS
6-8 week female BALB/c mice were randomly grouped and immunized three times intranasally with 10(8) TCID50 rvAdVP6(o) and rvAdVP6, respectively, then detect the serum IgG level against rotavirus induced by rvAdVP6(o) and rvAdVP6. The amount of sheding viral antigens in feces was detectd after mice rotavirus was taked orally.


RESULTS
The serum IgG level against rotavirus induced by rvAdVP6(o) was higher than that of rvAdVP6 after three times of immunization. The immunized mice shed lower amount of viral antigens in feces as compared with the rvAdVP6.


CONCLUSIONS
The recombinant adenovirus which encode optimized human rotavirus VP6 proteins (rvAdVP6(o)) could induce stronger serum immune and protective responses against the challenge of the rotavirus than the wild type (rvAdVP6) at the same immunizing dosage.
Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
Min
M
Wang
Feng
F
Wu
Jing-Dong
JD
Song
Jian-Guo
JG
Qu
Jian-Wei
JW
Wang
Tao
T
Hong
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology; D003062:Codon / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D012400:Rotavirus Infections / Q000517:prevention & control*; D022243:Rotavirus Vaccines / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D003062:Codon; D007074:Immunoglobulin G; D022243:Rotavirus Vaccines; C061332:VP6 protein, Rotavirus; D014614:Vaccines, Synthetic