Application of an omonasteine ligation strategy for the total chemical synthesis of the BRD7 bromodomain.
The use of omonasteine (Omo) in sequential peptide ligation strategies extends the scope of homocysteine (Hcy) ligation to longer, methionine-rich proteins. Hcy-to-Omo conversion can be performed on-resin, while the Omo-to-Hcy deprotection can be performed in situ after peptide ligation. This strategy was successfully applied in the synthesis of the BRD7 bromodomain.
Laboratory for Chemical Protein Synthesis, Department of Biochemistry, Maastricht University, Universiteitssingel 50, 6200 MD, Maastricht, The Netherlands. p.vandevijver@maastrichtuniversity.nl
Pieter
P
Van de Vijver
Liesbeth
L
Scheer
Judy
J
van Beijnum
Arjan
A
Griffioen
Tilman M
TM
Hackeng
D000595:Amino Acid Sequence; D060326:Chemistry Techniques, Synthetic; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry*; D006710:Homocysteine / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary
D002868:Chromosomal Proteins, Non-Histone; D006710:Homocysteine