Layered nanoprobe for long-lasting fluorescent cell label.
A long-lasting particle-based fluorescent label is designed for extended cell imaging studies. This onion-like nanoprobe is constructed through layer-by-layer fabrication technology. The nanoprobes are assembled with multiple layers of optically quenched polyelectrolytes, the fluorescence signal of which can be released later by intracellular proteolysis. Upon incubation with cells, the assembled nanoprobes are taken up efficiently. The tight packing and layered assembly of the quenched polyelectrolytes slow subsequent intracellular degradation, and then result in a prolonged intracellular fluorescence signal for up to 3 weeks with no noticeable toxicity.
Department of Translational Imaging, The Methodist Hospital Research Institute, Weill Cornell Medical College, Houston, TX 77030, USA.
Seung Koo
SK
Lee
Myung Shin
MS
Han
Ching-Hsuan
CH
Tung
D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002477:Cells / Q000187:drug effects / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D010641:Phenotype; D012235:Rhodamines / Q000378:metabolism; D013194:Staining and Labeling; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
C116572:(((4-chloromethyl)benzoyl)amino)-tetramethylrhodamine; D005456:Fluorescent Dyes; D012235:Rhodamines; D006046:Gold