A MYB transcription factor from the grey mangrove is induced by stress and confers NaCl tolerance in tobacco.
MYB transcription factor genes play important roles in developmental and various other processes in plants. In this study, functional characterization of AmMYB1, a single-repeat MYB transcription factor isolated from the salt-tolerant mangrove tree Avicennia marina is reported. AmMYB1 cDNA was 1046 bp in length with an open reading frame of 783 bp, encoding 260 amino acids. The corresponding gene had two introns and three exons and was present as a single copy in A. marina. The deduced amino acid sequence showed similarities to MYB proteins reported in other plants, including the conserved MYB binding domain. RNA gel blot analysis showed that the AmMYB1 transcript expression was more pronounced in green photosynthetic tissue and was strongly induced by stresses such as salt (500 mM), light (500 µE m(-2) s(-1)), and the exogenous application of ABA (100 µM). An analysis of the upstream sequence of AmMYB1 gene revealed the presence of regulatory elements identical to those present in the promoters of stress inducible genes. The promoter was responsive to NaCl and could enhance reporter gene expression in planta. An in vitro DNA binding assay using the promoter region (TGGTTAG) of the AtRD22 gene and a transactivation assay in yeast cells suggest the possibility of AmMYB1 protein regulating the expression of other genes during salt stress. Transgenic tobacco plants constitutively expressing the AmMYB1 transcription factor showed better tolerance to NaCl stress.
M. S. Swaminathan Research Foundation, Biotechnology, Taramani, Chennai 600113,  India.
G
G
Ganesan
H M
HM
Sankararamasubramanian
M
M
Harikrishnan
Ashwin
A
Ganpudi
G
G
Ashwin
Ajay
A
Parida
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D032407:Avicennia / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000235:genetics; D008027:Light; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D018547:Plant Stems / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D055049:Salt Tolerance; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D016415:Sequence Alignment; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; D012965:Sodium Chloride; D000040:Abscisic Acid