Angiotensin-converting enzyme-inhibitory activity in protein hydrolysates from normal and anthracnose disease-damaged Phaseolus vulgaris seeds.
BACKGROUND
Bean seeds are an inexpensive source of protein. Anthracnose disease caused by the fungus Colletotrichum lindemuthianum results in serious losses in common bean (Phaseolus vulgaris L.) crops worldwide, affecting any above-ground plant part, and protein dysfunction, inducing the synthesis of proteins that allow plants to improve their stress tolerance. The aim of this study was to evaluate the use of beans damaged by anthracnose disease as a source of peptides with angiotensin-converting enzyme (ACE-I)-inhibitory activity.


RESULTS
Protein concentrates from beans spoiled by anthracnose disease and from regular beans as controls were prepared by alkaline extraction and precipitation at isolelectric pH and hydrolysed using Alcalase 2.4 L. The hydrolysates from spoiled beans had ACE-I-inhibitory activity (IC(50) 0.0191 mg protein mL(-1)) and were very similar to those from control beans in terms of ACE-I inhibition, peptide electrophoretic profile and kinetics of hydrolysis. Thus preparation of hydrolysates using beans affected by anthracnose disease would allow for revalorisation of this otherwise wasted product.


CONCLUSIONS
The present results suggest the use of spoiled bean seeds, e.g. anthracnose-damaged beans, as an alternative for the isolation of ACE-I-inhibitory peptides to be further introduced as active ingredients in functional foods.
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Col. Casco de Santo Tomás, Del. Miguel Hidalgo, 11340 México, DF, Mexico.
Alan Javier
AJ
Hernández-Álvarez
Janet
J
Carrasco-Castilla
Gloria
G
Dávila-Ortiz
Manuel
M
Alaiz
Julio
J
Girón-Calle
Javier
J
Vioque-Peña
Carmen
C
Jacinto-Hernández
Cristian
C
Jiménez-Martínez
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D005658:Fungi; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D027805:Phaseolus / Q000737:chemistry* / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology*; D010940:Plant Proteins / Q000378:metabolism / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000378:metabolism / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry* / Q000382:microbiology
D000806:Angiotensin-Converting Enzyme Inhibitors; D010455:Peptides; D010940:Plant Proteins; D011492:Protein Hydrolysates; D007703:Peptidyl-Dipeptidase A