Peptide isolated from Japanese flounder skin gelatin protects against cellular oxidative damage.
Gelatin was extracted from the skin of Japanese flounder ( Palatichtys olivaceus ) and was subjected to enzymatic hydrolysis. The peptic hydrolysate resulted in a potent antioxidative peptide Gly-Gly-Phe-Asp-Met-Gly (582 Da), which bears +12.61 kcal/mol hydrophobicity. The antioxidative potential of the peptide was characterized by analyzing the protective effect of the peptide on reactive oxygen species (ROS)-mediated intracellular macromolecule damage. It was found that the peptide is a potent scavenger of intracellular ROS, thereby protecting the radical-mediated damage of membrane lipids, proteins, and DNA. Moreover, the peptide is capable of upregulating the expression of inherent antioxidative enzymes, superoxide dismutase-1, glutathione, and catalase. Collectively, it can be concluded that Japanese flounder skin, a processing byproduct of filleting, can be effectively used to produce a bioactive peptide with potent antioxidant capacity.
Marine Biochemistry Laboratory, Department of Chemistry, Pukyong National University , Busan 608-737, South Korea.
S W A
SW
Himaya
BoMi
B
Ryu
Dai-Hung
DH
Ngo
Se-Kwon
SK
Kim
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D029941:Fish Proteins / Q000737:chemistry* / Q000378:metabolism; D005432:Flounder / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D012867:Skin / Q000737:chemistry* / Q000378:metabolism
D000975:Antioxidants; D029941:Fish Proteins; D010455:Peptides; D020011:Protective Agents; D005780:Gelatin