Effects of simulated space radiation on immunoassay components for life-detection experiments in planetary exploration missions.
The Life Marker Chip (LMC) instrument is part of the proposed payload on the ESA ExoMars rover that is scheduled for launch in 2018. The LMC will use antibody-based assays to detect molecular signatures of life in samples obtained from the shallow subsurface of Mars. For the LMC antibodies, the ability to resist inactivation due to space particle radiation (both in transit and on the surface of Mars) will therefore be a prerequisite. The proton and neutron components of the mission radiation environment are those that are expected to have the dominant effect on the operation of the LMC. Modeling of the radiation environment for a mission to Mars led to the calculation of nominal mission fluences for proton and neutron radiation. Various combinations and multiples of these values were used to demonstrate the effects of radiation on antibody activity, primarily at the radiation levels envisaged for the ExoMars mission as well as at much higher levels. Five antibodies were freeze-dried in a variety of protective molecular matrices and were exposed to various radiation conditions generated at a cyclotron facility. After exposure, the antibodies' ability to bind to their respective antigens was assessed and found to be unaffected by ExoMars mission level radiation doses. These experiments indicated that the expected radiation environment of a Mars mission does not pose a significant risk to antibodies packaged in the form anticipated for the LMC instrument.
Cranfield Health, Cranfield University, Cranfield, Bedfordshire, United Kingdom.
Mariliza
M
Derveni
Alex
A
Hands
Marjorie
M
Allen
Mark R
MR
Sims
David C
DC
Cullen
D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D003359:Cosmic Radiation; D004797:Enzyme-Linked Immunosorbent Assay; D018559:Exobiology; D005118:Extraterrestrial Environment; D007118:Immunoassay; D011829:Radiation Dosage; D013026:Space Flight
D000906:Antibodies