In vivo imaging of nitric oxide and reactive oxygen species using laser scanning confocal microscopy.
Both nitric oxide (NO) and reactive oxygen species (ROS) are versatile molecules that mediate a variety of cellular responses in plants. In this chapter, methods for imaging NO and ROS using laser scanning confocal microscopy (LSCM) are presented. Arabidopsis roots, dyed with DAF-FM or H(2)DCF, are observed using the Leica TCS-SP2 LSCM. NO or ROS production are imaged and their kinetic changes monitored with the laser excitation and emission wavelengths at 488 nm and between 500 and 530 nm, respectively. In addition, Leica software is employed to visualize and calculate the fluorescence intensity data.
College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Yan-Jie
YJ
Xie
Wen-Biao
WB
Shen
D017360:Arabidopsis / Q000502:physiology; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods; D009569:Nitric Oxide / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism* / Q000502:physiology; D013050:Spectrometry, Fluorescence
D017382:Reactive Oxygen Species; D009569:Nitric Oxide