Incorporation of 4'-C-aminomethyl-2'-O-methylthymidine into DNA by thermophilic DNA polymerases.
The dual modified nucleotide 4'-C-aminomethyl-2'-O-methylthymidine 5'-triphosphate was synthesized and enzymatically incorporated into DNA by the thermophilic DNA polymerases Pfu and Therminator III. The dual ribose modification imparted increased exonuclease resistance to DNA compared to the well-known 2'-O-methyl modification.
Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Ganesh N
GN
Nawale
Kiran R
KR
Gore
Claudia
C
Höbartner
P I
PI
Pradeepkumar
D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism; D009690:Nucleic Acid Conformation; D013696:Temperature; D013936:Thymidine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism
C577203:4'-C-aminomethyl-2'-O-methylthymidine; D004247:DNA; D004259:DNA-Directed DNA Polymerase; D013936:Thymidine