Assignment of the backbone carbonyl resonances in (15)N-labelled proteins with           (13)C at natural abundance by a 2D triple-resonance correlation technique.
A 2D NMR experiment for assignment of backbone carbon resonances in small and medium-sized (15)N-labelled proteins with (13)C at natural abundance is presented. The experiment is a two-dimensional variant of the HNCO triple-resonance experiment and is demonstrated by application to a 6 kDa protein at relatively low concentration (2 mM) and temperature (30°C). The experiment is particularly suitable for assignment of carbonyl resonances.
Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100, Copenhagen Ø, Denmark.
S M
SM
Kristensen
M D
MD
Sørensen
J J
JJ
Led