The assembly domain of the small capsid protein of Kaposi's sarcoma-associated herpesvirus.
Self-assembly of Kaposi's sarcoma-associated herpesvirus capsids occurs when six proteins are coexpressed in insect cells using recombinant baculoviruses; however, if the small capsid protein (SCP) is omitted from the coinfection, assembly does not occur. Herein we delineate and identify precisely the assembly domain and the residues of SCP required for assembly. Hence, six residues, R14, D18, V25, R46, G66, and R70 in the assembly domain, when changed to alanine, completely abolish or reduce capsid assembly.
Viral Oncology Program, The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University, Baltimore, Maryland, USA.
Dale
D
Kreitler
Christopher M
CM
Capuano
Brandon W
BW
Henson
Erin N
EN
Pryce
Daniel
D
Anacker
J Michael
JM
McCaffery
Prashant J
PJ
Desai
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D016367:Baculoviridae; D002460:Cell Line; D005822:Genetic Vectors; D019288:Herpesvirus 8, Human / Q000235:genetics* / Q000502:physiology*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000235:genetics / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D016415:Sequence Alignment; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism*; D014771:Virion / Q000648:ultrastructure; D019065:Virus Assembly
D050505:Mutant Proteins; C119019:ORF65 protein, human herpesvirus 8; D014764:Viral Proteins