Synthesis, characterization, cellular uptake, apoptosis, cytotoxicity, dna-binding, and antioxidant activity studies of ruthenium(II) complexes.
Two new ruthenium(II) polypyridyl complexes [Ru(dmb)(2)(HECIP)](ClO(4))(2) (1) (HECIP = N-ethyl-4-[(1,10)-phenanthroline(5,6-f)imidazol-2-yl]carbazole, dmb = 4,4'-dimethyl-2,2'-bipyridine) and [Ru(dmp)(2)(HECIP)](ClO(4))(2) (2) (dmp = 2,9-dimethyl-1,10-phenanthroline) have been synthesized and characterized. The DNA-binding behaviors of the two complexes were investigated by absorption spectra, viscosity measurements, and photoactivated cleavage. The DNA-binding constants for complexes 1 and 2 were determined to be 8.03 (± 0.12) × 10(4) M(-1) (s = 1.62) and 2.97 (± 0.15) × 10(4) M(-1) (s = 1.82), respectively. The results suggest that these complexes interact with DNA through intercalative mode. The photocleavage of pBR322 DNA by Ru(II) complexes was investigated. The cytotoxicity of complexes 1 and 2 has been evaluated by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide)] method. Complex 1 shows higher anticancer potency than 2 against the four tumor cell lines. Apoptosis and cellular uptake were investigated. The antioxidant activities of the ligand and these complexes were also performed.
School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan, PR China.
Li
L
Xu
Nan-Jing
NJ
Zhong
Hong-Liang
HL
Huang
Zhen-Hua
ZH
Liang
Zheng-Zheng
ZZ
Li
Yun-Jun
YJ
Liu
D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D053837:DNA Cleavage; D006801:Humans; D010084:Oxidation-Reduction; D012428:Ruthenium / Q000737:chemistry; D014783:Viscosity
D000975:Antioxidants; D056831:Coordination Complexes; D012428:Ruthenium; D004247:DNA