Chitosan-heparin polyelectrolyte multilayers on cortical bone: periosteum-mimetic, cytophilic, antibacterial coatings.
Cortical bone allografts suffer from high rates of failure due to poor integration with host tissue, leading to non-union, fracture, and infection following secondary procedures. Here, we report a method for modifying the surfaces of cortical bone with coatings that have biological functions that may help overcome these challenges. These chitosan-heparin coatings promote mesenchymal stem cell attachment and have significant antibacterial activity against both S. aureus and E. coli. Furthermore, their chemistry is similar to coatings we have reported on previously, which effectively stabilize and deliver heparin-binding growth factors. These coatings have potential as synthetic periosteum for improving bone allograft outcomes.
Grenoble Institute of Technology, LMGP, CNRS, Grenoble, France.
Jorge
J
Almodóvar
Justin
J
Mower
Apurba
A
Banerjee
Ajoy K
AK
Sarkar
Nicole P
NP
Ehrhart
Matt J
MJ
Kipper
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D016025:Bone Transplantation / Q000379:methods*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D005227:Fatty Acids; D005260:Female; D005269:Femur; D006493:Heparin / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010521:Periosteum / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D012756:Sheep; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005227:Fatty Acids; C016173:undecanoic acid; D006493:Heparin; D048271:Chitosan