Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array.
We have fabricated surface-enhanced Raman scattering (SERS) substrates based on arrays of silver nanoparticles grown on porous anodic alumina templates. Using this nanotechnology platform, label-free and high-speed detection of bacteria are achieved. SERS spectra of various bacteria including Staphylococcus Aureus (Gram-positive bacterium), Klebsiella Pneumoniae (Gram-negative bacterium), and Mycobacterium Smegmatis (Mycobacterium) were recorded. The highly reproducible SERS-based technological platform is capable of differentiating different kinds of bacteria by PCA, LDA, clustering analysis, and SVM methods, which provides promising opportunity for biosensing of clinical microbes.
Institute of Atomic and Molecular Science, Academia Sinica, Taipei 10617, Taiwan.
T Y
TY
Liu
Y
Y
Chen
H H
HH
Wang
Y L
YL
Huang
Y C
YC
Chao
K T
KT
Tsai
W C
WC
Cheng
C Y
CY
Chuang
Y H
YH
Tsai
C Y
CY
Huang
D W
DW
Wang
C H
CH
Lin
J K
JK
Wang
Y L
YL
Wang
D001419:Bacteria / Q000737:chemistry / Q000302:isolation & purification*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002462:Cell Membrane / Q000737:chemistry; D015169:Colony Count, Microbial / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008027:Light; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation*; D012542:Scattering, Radiation; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*