Comparative cytotoxicity study of water-soluble carbon nanoparticles on plant cells.
This study provides a parallel cytotoxicity study of water-soluble single-walled carbon nanotubes and fullerene C70(C(COOH)2)4-8 on Nicotiana tabacum BY-2 cells. After BY-2 cells were treated with single-walled carbon nanotubes or C70(C(COOH)2)4-8, the cells adopted a shrunken morphology and exhibited lower proliferation rate. Fluorescence imaging of the treated cells revealed increased reactive oxygen species generation, abnormal mitochondrial distribution, decreased mitochondrial activity, and impaired actin cytoskeleton arrangement. However, no obvious cells death was induced by either single-walled carbon nanotubes or C70(C(COOH)2)4-8. Compared with single-walled carbon nanotubes, C70(C(COOH)2)4-8 exerted more serious adverse effects on BY-2 cells.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
Song
S
Dang
Qiaoling
Q
Liu
Xuejie
X
Zhang
Kangmin
K
He
Chunru
C
Wang
Xiaohong
X
Fang
D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D008422:Materials Testing; D037742:Nanotubes, Carbon / Q000009:adverse effects* / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012995:Solubility; D014026:Nicotiana / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D014867:Water / Q000737:chemistry
D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; D014867:Water