Blood cells attachment after root conditioning and PRP application: an in vitro study.
OBJECTIVE
Root conditioning is aimed at smear layer removal and at dental matrix collagen exposure, which may promote periodontal regeneration. This in vitro study assessed smear layer removal, collagen fiber exposure and the influence of PRP (platelet-rich plasma) application on adhesion of blood cells to the root surface using scanning electron microscopy (SEM).


METHODS
Scaled root samples (n = 160) were set in five groups and conditioned with: group I - control group (saline solution); group II (EDTA 24%); group III (citric acid 25%); group IV (tetracycline hydrochloride 50 mg/ml); group V (sodium citrate 30%). Eighty samples were assessed using the root surface modification index (RSMI). The other eighty samples were set in two groups. The first group (n = 40) received PRP gel application with a soft brush and the second group (n = 40) received PRP application and then a blood drop. The fibrin clot formation was assessed in the first group and the blood cells adhesion was assessed in the second group using the BEAI (blood elements adhesion index). A previously trained, calibrated, and blind examiner evaluated photomicrographs. Statistical analysis was performed using the Kruskal-Wallis's and Dunn's tests.


RESULTS
Group III attained the best results for RSMI and BEAI. Moreover, it was the only group showing fibrin clot formation.


CONCLUSIONS
Citric acid was the most efficient conditioner for smear layer removal, collagen fiber exposure and blood cell adhesion. Moreover, it was the only group showing fibrin clot formation after PRP application.


CONCLUSIONS
This study demonstrated that root conditioning followed by PRP application may favor blood cell adhesion on root surface which may optimize periodontal healing.
Department of Operative Dentistry, School of Dentistry at Araraquara, State University of Sao Paulo, UNESP, 1680 Humaita Street, Araraquara, 14801-903, Sao Paulo, Brazil. aarached@yahoo.com.br
Andrea Abi Rached
AA
Dantas
Lucas Amaral
LA
Fontanari
Eduardo de Paula
Ede P
Ishi
Fabio Renato Manzolli
FR
Leite
Daniela Leal
DL
Zandim
Ricardo Samih Georges Abi
RS
Rached
Jose Eduardo Cezar
JE
Sampaio
D001773:Blood Cells / Q000502:physiology*; D001777:Blood Coagulation; D002448:Cell Adhesion; D002614:Chelating Agents / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D003739:Dental Cementum / Q000187:drug effects / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects* / Q000648:ultrastructure; D004492:Edetic Acid / Q000494:pharmacology; D005337:Fibrin / Q000502:physiology; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D053657:Platelet-Rich Plasma; D016369:Smear Layer; D000077559:Sodium Citrate; D013752:Tetracycline / Q000494:pharmacology; D014016:Tissue Conditioning, Dental; D017001:Tooth Demineralization; D014092:Tooth Root / Q000187:drug effects*
D002614:Chelating Agents; D002951:Citrates; D000077559:Sodium Citrate; D019343:Citric Acid; D005337:Fibrin; D004492:Edetic Acid; D013752:Tetracycline