(24S)-ergostane-3β,5α,6β-triol from Hedyotis chrysotricha with inhibitory activity on migration of SK-HEP-1 human hepatocarcinoma cells.
The methanol extract of the whole plant of Hedyotis chrysotricha demonstrated cytotoxicity against SK-HEP-1 human hepatocarcinoma cells in a primary screening for novel antitumour agents. Bioassay-guided fractionation and purification led to an active principle (24S)-ergostane-3β,5α,6β-triol (1) along with four inactive compounds (2-5). The in vitro transwell migration assay showed that compound 1 remarkably reduced the migration of SK-HEP-1 cells by 78.9% at a dose of 30 µM, without any apoptotic effect on this cell line. Moreover, all the isolated compounds were further evaluated for their cytotoxicities against another four human cancer cell lines (MCF-7, NUGC3, SH-SY5Y and PC-3).
Department of Natural Products for Chemical Genetic Research, Key Laboratory of Brain Functional Genomics, East China Normal University, 3663 Zhongshan Road N, Shanghai 200062, PR China.
Miao
M
Ye
Jing-Jing
JJ
Su
Shu-Ting
ST
Liu
Lei
L
Cao
Juan
J
Xiong
Yun
Y
Zhao
Hui
H
Fan
Guo-Xun
GX
Yang
Gang
G
Xia
Jin-Feng
JF
Hu
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D032064:Hedyotis / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry
C586171:(24S)-ergostane-3beta,5alpha,6beta-triol; D000972:Antineoplastic Agents, Phytogenic; C115535:pomolic acid; D009828:Oleanolic Acid; D004875:Ergosterol