The potential of a niacinamide dominated cosmeceutical formulation on fibroblast activity and wound healing in vitro.
Knowledge on the intrinsic mechanisms involved in wound healing provides opportunity for various therapeutic strategies. The manipulation of dermal fibroblast proliferation and differentiation might prove to beneficially augment wound healing. This study evaluated the combined effects of niacinamide, L-carnosine, hesperidin and Biofactor HSP(®) on fibroblast activity. The effects on fibroblast collagen production, cellular proliferation, migration and terminal differentiation were assessed. In addition, the authors determined the effects on in vitro wound healing. The optimal concentrations of actives were determined in vitro. Testing parameters included microscopic morphological cell analysis, cell viability and proliferation determination, calorimetric collagen detection and in vitro wound healing dynamics. Results show that 0·31 mg/ml niacinamide, 0·10 mg/ml L-carnosine, 0·05 mg/ml hesperidin and 5·18 µg/ml Biofactor HSP® proved optimal in vitro. The results show that fibroblast collagen synthesis was increased alongside with cellular migration and proliferation.
Department of Anatomy, School of Medicine, University of Namibia, Windhoek, NamibiaDepartment of Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, South AfricaSouthern group of Companies, Southern Implants Office Park, Irene, South Africa.
Quenton
Q
Wessels
Etheresia
E
Pretorius
Celeste M
CM
Smith
Hugo
H
Nel
D002336:Carnosine / Q000494:pharmacology; D002468:Cell Physiological Phenomena / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003358:Cosmetics / Q000494:pharmacology*; D020405:Dermis / Q000166:cytology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000494:pharmacology; D006569:Hesperidin / Q000494:pharmacology; D006801:Humans; D009536:Niacinamide / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
D003358:Cosmetics; D004338:Drug Combinations; D018840:HSP70 Heat-Shock Proteins; D009536:Niacinamide; D002336:Carnosine; D003094:Collagen; D006569:Hesperidin