Lack of functional effects of neuromuscular electrical stimulation on skeletal muscle oxidative metabolism in healthy humans.
A recent study has demonstrated that neuromuscular electrical stimulation (NMES) determines, in vitro, a fast-to-slow shift in the metabolic profile of muscle fibers. The aim of the present study was to evaluate if, in the same subjects, these changes would translate, in vivo, into an enhanced skeletal muscle oxidative metabolism. Seven young men were tested (cycle ergometer) during incremental exercises up to voluntary exhaustion and moderate and heavy constant-load exercises (CLE). Measurements were carried out before and after an 8-wk training program by isometric bilateral NMES (quadriceps muscles), which induced an ∼25% increase in maximal isometric force. Breath-by-breath pulmonary O(2) uptake (Vo(2)) and vastus lateralis oxygenation indexes (by near-infrared spectroscopy) were determined. Skeletal muscle fractional O(2) extraction was estimated by near-infrared spectroscopy on the basis of changes in concentration of deoxygenated hemoglobin + myoglobin. Values obtained at exhaustion were considered "peak" values. The following functional evaluation variables were unaffected by NMES: peak Vo(2); gas exchange threshold; the Vo(2) vs. work rate relationship (O(2) cost of cycling); changes in concentration of deoxygenated hemoglobin + myoglobin vs. work rate relationship (related to the matching between O(2) delivery and Vo(2)); peak fractional O(2) extraction; Vo(2) kinetics (during moderate and heavy CLE) and the amplitude of its slow component (during heavy CLE). Thus NMES did not affect several variables of functional evaluation of skeletal muscle oxidative metabolism. Muscle hypertrophy induced by NMES could impair peripheral O(2) diffusion, possibly counterbalancing, in vivo, the fast-to-slow phenotypic changes that were observed in vitro, in a previous work, in the same subjects of the present study.
Institute of Bioimaging and Molecular Physiology, Consiglio Nazionale delle Ricerche, Segrate, Milan, Italy.
Simone
S
Porcelli
Mauro
M
Marzorati
Lorenzo
L
Pugliese
Saverio
S
Adamo
Julien
J
Gondin
Roberto
R
Bottinelli
Bruno
B
Grassi
D000328:Adult; D004558:Electric Stimulation / Q000379:methods; D015444:Exercise / Q000502:physiology; D005080:Exercise Test / Q000379:methods; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006984:Hypertrophy / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology*; D009211:Myoglobin / Q000378:metabolism; D009469:Neuromuscular Junction / Q000378:metabolism / Q000502:physiology*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D011659:Pulmonary Gas Exchange / Q000502:physiology
D006454:Hemoglobins; D009211:Myoglobin; D010100:Oxygen