Analysis of the contribution of nonresident progenitor cells and hematopoietic cells to reparative dentinogenesis using parabiosis model in mice.
BACKGROUND
The aim of this study was to analyze the contribution of nonresident progenitor/stem cells and hematopoietic cells to reparative dentinogenesis.


METHODS
Parabiosis was established between C57BL/6-TgN(ACTbEGFP)10sb/J transgenic mice (GFP+) and C57BL/6 wild-type mice (GFP-) to ensure blood cross-circulation between animals. Reparative dentinogenesis was stimulated by pulp exposures and capping on the first maxillary molar in the GFP- mice. Histologic sections of injured molars from GFP- mice were analyzed by epifluorescence microscopy to examine the contributions of GFP+ cells (nonresident progenitor cells and hematopoietic cells originating from GFP+ mice) to reparative dentinogenesis.


RESULTS
GFP+ cells were detected in close association with reparative dentin formed at the site of pulp exposure in the maxillary first molars of the GFP- mice.


CONCLUSIONS
The present study suggests the participation of the nonresident progenitor cells and hematopoietic cells in reparative dentinogenesis.
Department of Restorative Dentistry, Division of Endodontics, Dental School of Piracicaba, State University of Campinas, São Paulo, São Paulo, Brazil. marcos.rfrozoni@terra.com.br
Marcos
M
Frozoni
Alexandre Augusto
AA
Zaia
Sergio Roberto Peres
SR
Line
Mina
M
Mina
D000135:Acid Phosphatase / Q000032:analysis; D017607:Aluminum Compounds / Q000627:therapeutic use; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D017610:Calcium Compounds / Q000627:therapeutic use; D003188:Composite Resins / Q000737:chemistry; D003427:Cross Circulation / Q000379:methods; D003764:Dental Materials / Q000737:chemistry; D003785:Dental Pulp Capping / Q000379:methods; D003789:Dental Pulp Exposure / Q000473:pathology / Q000628:therapy; D003809:Dentin, Secondary / Q000502:physiology; D003810:Dentinogenesis / Q000502:physiology*; D004338:Drug Combinations; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007527:Isoenzymes / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D008963:Molar / Q000473:pathology / Q000503:physiopathology; D009804:Odontoblasts / Q000473:pathology; D010087:Oxides / Q000627:therapeutic use; D010227:Parabiosis / Q000379:methods*; D057872:Pulp Capping and Pulpectomy Agents / Q000627:therapeutic use; D019279:Resin Cements / Q000737:chemistry; D017640:Silicates / Q000627:therapeutic use; D012822:Silicon Dioxide / Q000737:chemistry; D013234:Stem Cells / Q000502:physiology*; D000071681:Tartrate-Resistant Acid Phosphatase; D015040:Zirconium / Q000737:chemistry
D017607:Aluminum Compounds; D015415:Biomarkers; D017610:Calcium Compounds; C438313:Clearfil SE Bond; D003188:Composite Resins; D003764:Dental Materials; D004338:Drug Combinations; D005456:Fluorescent Dyes; D007527:Isoenzymes; D010087:Oxides; D057872:Pulp Capping and Pulpectomy Agents; D019279:Resin Cements; D017640:Silicates; C083568:Z100 composite resin; C485184:enhanced green fluorescent protein; C086631:mineral trioxide aggregate; D049452:Green Fluorescent Proteins; D012822:Silicon Dioxide; D015040:Zirconium; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase