Noninvasive evaluation of collagen and hemoglobin contents and scattering property of in vivo keloid scars and normal skin using diffuse reflectance spectroscopy: pilot study.
Collagen is a rich component in skin that provides skin structure integrity; however, its contribution to the absorption and scattering properties of various types of skin has not been extensively studied. We considered the contribution of the collagen to the absorption spectrum of in vivo normal skin and keloids of 12 subjects derived from our diffuse reflectance spectroscopy (DRS) system in the wavelength range from 550 to 860 nm. It was found that the collagen concentration, the hemoglobin oxygen saturation, and the reduced scattering coefficient of keloids were remarkably different from that of normal skin. Our results suggest that our DRS system could assist clinicians in understanding the functional and structural condition of keloid scars. In the future, we will evaluate the accuracy of our system in the keloid diagnosis and investigate the applicability of our system for other skin-collagen-related studies.
National Cheng-Kung University, Department of Photonics, Tainan 701, Taiwan. stseng@mail.ncku.edu.tw
Sheng-Hao
SH
Tseng
Chao-Kai
CK
Hsu
Julia
J
Yu-Yun Lee
Shih-Yu
SY
Tzeng
Wan-Rung
WR
Chen
Yu-Kai
YK
Liaw
D000153:Acne Keloid / Q000175:diagnosis* / Q000378:metabolism*; D015415:Biomarkers / Q000032:analysis; D003094:Collagen / Q000032:analysis*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008027:Light; D012016:Reference Values; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012867:Skin / Q000737:chemistry*; D013057:Spectrum Analysis / Q000379:methods*
D015415:Biomarkers; D006454:Hemoglobins; D003094:Collagen