Iodobacter limnosediminis sp. nov., isolated from Arctic lake sediment.
A Gram-reaction-negative, motile, non-violet-pigmented, rod-shaped bacterial strain, designated E1(T), was isolated from Arctic lake sediment. Growth occurred at 4 °C-28 °C (optimum, 18 °C), at pH 4-11(optimum, 9-10) and in the presence of 0-1 % (w/v) NaCl. The taxonomic position of E1(T) was analysed using a polyphasic approach. Strain E1(T) exhibited 16S rRNA gene sequence similarity value of 98.1 % with respect to the type strain of Iodobacter fluviatilis, but no more than 93 % with the type strains of other recognized species. A further DNA-DNA hybridization experiment was conducted, which demonstrated unambiguously that strain E1(T) was distinct from I. fluviatilis ATCC 33051(T) (51.3 % relatedness). The DNA G+C content of strain E1(T) was 52.3 mol%. Chemotaxonomic data [Q-8 as the monospecific respiratory quinone and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c, 56.1 %) and C16 : 0 (18.8 %) as the major cellular fatty acids] supported the affiliation of strain E1(T) to the genus Iodobacter. However, the results of physiological and biochemical tests allowed phenotypic differentiation of strain E1(T) from I. fluviatilis ATCC 33051(T). On the basis of phenotypic and genotypic properties, strain E1(T) represents a novel species of genus Iodobacter, for which the name Iodobacter limnosediminis sp. nov. is proposed. The type strain is E1(T) ( = CCTCC AB 2010224(T) = NRRL B-59456(T)).
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Wei
W
Su
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Zhichao
Z
Zhou
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Fan
F
Jiang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
XuLu
X
Chang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Ying
Y
Liu
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Shaohua
S
Wang
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Wenjing
W
Kan
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Mengchen
M
Xiao
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Ming
M
Shao
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Fang
F
Peng
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Chengxiang
C
Fang
D001110:Arctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009346:Neisseriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S