A new biosensor for chiral recognition using goat and rabbit serum albumin self-assembled quartz crystal microbalance.
Quartz crystal microbalance (QCM) biosensor was used for the chiral recognition of five pairs of enantiomers by using goat serum albumin (GSA) and rabbit serum albumin (RbSA) as chiral selectors. Serum albumin (SA) was immobilized on the QCM through the self-assembled monolayer technique, and the surface concentration of GSA and RbSA were 8.8 × 10(-12)   mol cm(-2) and 1.2 × 10(-11) mol cm(-2) , respectively. The QCM biosensors showed excellent sensitivity and selectivity. Meanwhile, the chiral recognition of SA sensors was quite species dependent. There were differences between GSA and RbSA sensors in the ability and the preference of chiral recognition. To R,S-1,2,3,4-tetrahydro-1-naphthylamine (R,S-1-TNA), R,S-1-(4-methoxyphenyl)ethylamine (R,S-4-MPEA), and R,S-1-(3-methoxyphenyl)ethylamine (R,S-3-MPEA), the preference of the stereoselective SA-drug binding of the two kinds of SA sensors were consistent. However, to R,S-2-octanol (R, S-2-OT) and R,S-methyl lactate (R,S-MEL), the two kinds of SA sensors had opposite chiral recognition preference. Moreover, the interactions of SA and the five pairs of enantiomers have been further investigated through ultraviolet (UV) and fluorescent (FL) spectra. The UV/FL results were in accordance with the consequence of QCM.
School of Chemistry and Environment, South China Normal University, Guangzhou, People's Republic of China.
Wen-Jing
WJ
Chen
Sheng
S
Zhang
Wei-Guang
WG
Zhang
Jun
J
Fan
Xia
X
Yin
Sheng-Run
SR
Zheng
Wen-Cui
WC
Su
Zhi
Z
Zhang
Tao
T
Hong
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D006041:Goats; D015394:Molecular Structure; D058525:Quartz Crystal Microbalance Techniques; D011817:Rabbits; D012709:Serum Albumin / Q000032:analysis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D012709:Serum Albumin