Protein analyses using differential ion mobility microchips with mass spectrometry.
Differential ion mobility spectrometry (FAIMS) integrated with mass spectrometry (MS) is a powerful new tool for biological and environmental analyses. Large proteins occupy regions of FAIMS spectra distinct from peptides, lipids, or other medium-size biomolecules, likely because strong electric fields align huge dipoles common to macroions. Here we confirm this phenomenon in separations of proteins at extreme fields using FAIMS chips coupled to MS and demonstrate their use to detect even minor amounts of large proteins in complex matrixes of smaller proteins and peptides.
Alexandre A
AA
Shvartsburg
Richard D
RD
Smith
D000818:Animals; D002417:Cattle; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D025801:Ubiquitin / Q000032:analysis
D007477:Ions; D011506:Proteins; D025801:Ubiquitin; D012710:Serum Albumin, Bovine