[Etiological and epidemiological study on viral diarrhea among children in Changchun].
OBJECTIVE
To study the pathogen and characteristics of viral diarrhea in children in Changchun area.


METHODS
460 stools specimens were collected from children with acute diarrhea cured in the childrens, hospital of Changchun in 2010. Rotavirus were detected by ELISA, caliceverus and astrovirus were detected by reverse transcription-polymerase chain reactions (RT-PCR), adenovirus were detected by polymerase chain reactions (PCR).


RESULTS
A total of 460 specimens were detected. The detection rate of rotavirus, caliceverus, astrovious, adenovious respectively is 35.22%, 20.43%, 9.78%, 3.70%, the detectablerate of mixed infection is 7.61%, children under 2 years old were the major patient. The main genotypes of the virus: rotavirus (G3P[8]), caliceverus (GII-4), astrovious (type I), adenovious (Ad41).


CONCLUSIONS
Rotavirus is the main pathogen in Changchun. Followed by caliceverus, astrovious, adenovious.
The First Hospital of Jilin University, Changchun 130021, China.
Xian-E
XE
Zhang
Dan-Di
DD
Li
Xiang
X
Li
Xian-Da
XD
Yang
Kun
K
Cai
Yong-Xia
YX
Wang
Li-Bin
LB
Yang
Zhaojun
Z
Duan
D000260:Adenoviruses, Human / Q000302:isolation & purification; D002139:Caliciviridae / Q000302:isolation & purification; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000209:etiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D001257:Mamastrovirus / Q000302:isolation & purification; D012401:Rotavirus / Q000302:isolation & purification; D014777:Virus Diseases / Q000453:epidemiology / Q000209:etiology*