Beta-cell autoimmunity.
Beta cell destruction in autoimmune diabetes is accompanied by the presence of autoantibodies and autoreactive T cells against beta cell antigens. Autoantibodies to insulin are predictive of future diabetes in man and in the non-obese diabetic mouse model. Furthermore, the detection of peripheral autoreactive CD8(+) T cells in this mouse model is indicative of beta cell killing and correlates with the development of diabetes. We describe two protocols that are helpful for the detection of beta-cell autoimmunity in mice. The first protocol describes the detection of insulin-specific autoantibodies using a radio-binding assay. The other is a general CD8(+) T cell ELISpot protocol for the detection of peptide-specific responses of CD8(+) T cells from secondary lymphoid organs or pancreatic islets.
Preclinical Approaches to Stem Cell Therapy/Diabetes, Technische Universität Dresden, DFG-Center for Regenerative Therapies Dresden, Cluster of Excellence, Dresden, Germany.
Yannick F
YF
Fuchs
Kerstin
K
Adler
Ezio
E
Bonifacio
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D015551:Autoimmunity; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000276:immunology*; D007328:Insulin / Q000276:immunology; D007330:Insulin Antibodies / Q000276:immunology*; D050417:Insulin-Secreting Cells / Q000276:immunology*; D051379:Mice; D016688:Mice, Inbred NOD; D011869:Radioligand Assay / Q000379:methods
D001323:Autoantibodies; D007328:Insulin; D007330:Insulin Antibodies