[Expression of human B-cell specific receptor FCRL1 in normal individuals and in patients with autoimmune diseases].
The comparative analysis of expression level of FCRL1 gene encoding human B-cell surface receptor in healthy individuals and patients with autoimmune diseases was carried out. For the expression estimation we used results of DNA dot-hybridization on the membranes, containing cDNA samples from subpopulations of blood cells of patients with autoimmune diseases. The quantitative estimation of hybridization signals showed that expression level of FCRL1 gene in peripheral blood B-lymphocytes was significantly higher in patients with a multiple sclerosis, lupus anticoagulans, Takayasu's arteritis and also in von Willebrand disease than in healthy individuals. FCRL1-specific monoclonal and polyclonal antibodies were raised. They were proven to detect FCRL1 in Western blotting, immunohistochemistry and flow cytometry. It was found that FCRL1 is expressed on the surface of CD19+ mature B-cells. In tonsil FCRL1-positive cells were located in crypt area: in mantle zone of secondary lymphoid follicles and among cells of lymphoepithelium. FCRL1-positive cells were also found in B-cell follicles of the spleen.
K O
KO
Baranov
O Iu
OIu
Volkova
L V
LV
Mechetina
N A
NA
Chikaev
E S
ES
Reshetnikova
G M
GM
Nikulina
A V
AV
Taranin
A M
AM
Naiakshin
D000906:Antibodies / Q000276:immunology; D018941:Antigens, CD19 / Q000276:immunology; D015551:Autoimmunity; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D016682:Lupus Coagulation Inhibitor / Q000235:genetics* / Q000276:immunology; D008565:Membrane Proteins / Q000235:genetics*; D009103:Multiple Sclerosis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D014066:Palatine Tonsil / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013625:Takayasu Arteritis / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D014842:von Willebrand Diseases / Q000235:genetics* / Q000276:immunology / Q000473:pathology
D000906:Antibodies; D018941:Antigens, CD19; D018076:DNA, Complementary; C551993:FCRL1 protein, human; D016682:Lupus Coagulation Inhibitor; D008565:Membrane Proteins