Cerebrospinal fluid biomarkers of neuropathologically diagnosed Parkinson's disease subjects.
OBJECTIVE
Parkinson's disease (PD) afflicts approximately 1-2% of the population over 50 years of age. No cures or effective modifying treatments exist and clinical diagnosis is currently confounded by a lack of definitive biomarkers. We sought to discover potential biomarkers in the cerebrospinal fluid (CSF) of neuropathologically confirmed PD cases.


METHODS
We compared postmortem ventricular CSF (V-CSF) from PD and normal control (NC) subjects using two-dimensional difference gel electrophoresis (2D-DIGE). Spots exhibiting a 1·5-fold or greater difference in volume between PD patients and controls were excised from the two-dimensional gels, subjected to tryptic digestion and identification of peptides assigned using mass spectrometric/data bank correlation methods.


RESULTS
Employing this strategy six molecules: fibrinogen, transthyretin, apolipoprotein E, clusterin, apolipoprotein A-1, and glutathione-S-transferase-Pi, were found to be different between PD and NC populations.


CONCLUSIONS
These molecules have been implicated in PD pathogenesis. Combining biomarker data from multiple laboratories may create a consensus panel of proteins that may serve as a diagnostic tool for this neurodegenerative disorder.
The Longtine Center for Neurodegenerative Biochemistry, Banner Sun Health Research Institute, Sun City, AZ, USA.
Chera L
CL
Maarouf
Thomas G
TG
Beach
Charles H
CH
Adler
Holly A
HA
Shill
Marwan N
MN
Sabbagh
Terence
T
Wu
Douglas G
DG
Walker
Tyler A
TA
Kokjohn
Alex E
AE
Roher
D000368:Aged; D000369:Aged, 80 and over; D016632:Apolipoprotein A-I / Q000134:cerebrospinal fluid; D001057:Apolipoproteins E / Q000134:cerebrospinal fluid; D015415:Biomarkers / Q000134:cerebrospinal fluid; D051152:Clusterin / Q000134:cerebrospinal fluid; D015331:Cohort Studies; D005260:Female; D005340:Fibrinogen / Q000134:cerebrospinal fluid; D051549:Glutathione S-Transferase pi / Q000134:cerebrospinal fluid; D006801:Humans; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis* / Q000473:pathology; D011228:Prealbumin / Q000134:cerebrospinal fluid; D020033:Protein Isoforms / Q000134:cerebrospinal fluid; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods
D016632:Apolipoprotein A-I; D001057:Apolipoproteins E; C115499:BBeta fibrinogen; D015415:Biomarkers; D051152:Clusterin; D011228:Prealbumin; D020033:Protein Isoforms; D005340:Fibrinogen; D051549:Glutathione S-Transferase pi