Experimental analysis of the mechanism of chromatin remodeling by RNA polymerase II.
The vital process of transcription by RNA polymerase II (Pol II) occurs in chromatin environment in eukaryotic cells; in fact, moderately transcribed genes retain nucleosomal structure. Recent studies suggest that chromatin structure presents a strong barrier for transcribing Pol II in vitro, and that DNA-histone interactions are only partially and transiently disrupted during transcript elongation on moderately active genes. Furthermore, elongating Pol II complex is one of the major targets during gene regulation. Below, we describe a highly purified, defined experimental system that recapitulates many important properties of transcribed chromatin in vitro and allows detailed analysis of the underlying mechanisms.
Department of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey, USA.
Daria A
DA
Gaykalova
Olga I
OI
Kulaeva
Nikolai A
NA
Pestov
Fu-Kai
FK
Hsieh
Vasily M
VM
Studitsky
D001483:Base Sequence; D042002:Chromatin Assembly and Disassembly; D004247:DNA / Q000737:chemistry; D018983:DNA Footprinting; D004262:DNA Restriction Enzymes / Q000737:chemistry; D057075:Enzyme Assays; D029968:Escherichia coli Proteins / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D006657:Histones / Q000737:chemistry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012313:RNA / Q000737:chemistry; D012319:RNA Polymerase II / Q000737:chemistry*; D061805:Transcription Elongation, Genetic
D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D006657:Histones; D009707:Nucleosomes; C034477:RNA primers; D012313:RNA; D004247:DNA; D012319:RNA Polymerase II; D004262:DNA Restriction Enzymes