Detection and isolation of dendritic cells using Lewis X-functionalized magnetic nanoparticles.
dendritic cell (DC)-specific intracellular adhesion molecule-3 grabbing nonintegrin (DC-SIGN) is a receptor found on DCs that recognizes antigens bearing mannose-rich or fucosylated glycans, including Lewis X (Le(X)). Here, we report the fabrication of magnetic nanoparticles coated with multivalent Le(X) glycans using Cu (I)-catalyzed azide-alkyne cycloaddition. The resulting nanoparticles are selective and biocompatible, serving as a highly efficient tool for DC detection and enrichment.
Department of Biochemistry, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA.
Sara H
SH
Rouhanifard
Ran
R
Xie
Guoxin
G
Zhang
Xiaoming
X
Sun
Xing
X
Chen
Peng
P
Wu
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002384:Catalysis; D015815:Cell Adhesion Molecules / Q000737:chemistry; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D003500:Cyclization; D003713:Dendritic Cells / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry; D016256:Lewis X Antigen / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000737:chemistry; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D000480:Alkynes; D001386:Azides; D015815:Cell Adhesion Molecules; D020099:Coated Materials, Biocompatible; D003300:Copper; D037181:Lectins, C-Type; D016256:Lewis X Antigen; D058185:Magnetite Nanoparticles; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin