Gemmobacter tilapiae sp. nov., a poly-β-hydroxybutyrate-accumulating bacterium isolated from a freshwater pond.
A novel bacterium, designated strain Ruye-53(T), was isolated from a freshwater pond used to rear tilapiine cichlid fish in Taiwan. The taxonomic position of the novel strain was determined using a polyphasic approach. Strain Ruye-53(T) was Gram-reaction-negative, aerobic, cream-white coloured, rod-shaped, non-motile and poly-β-hydroxybutyrate-accumulating. Growth occurred at 10-37 °C (optimum between 20 and 25 °C), at pH 6.0-9.0 (optimum between pH 8.0 and pH 9.0) and with 0-1 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ruye-53(T) belonged to the genus Gemmobacter and that its most closely related neighbour was Gemmobacter aquatilis DSM 3857(T) (97.6 % sequence similarity). The novel strain's predominant fatty acid was C18 : 1ω7c, its major respiratory quinone was Q-10 and its genomic DNA G+C content was 61.2 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one uncharacterized aminolipid and four uncharacterized phospholipids. The DNA-DNA relatedness value between strain Ruye-53(T) and Gemmobacter aquatilis DSM 3857(T) was about 45.5 % (48.2±0.4 % in the reciprocal experiment). On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Ruye-53(T) represents a novel species in the genus Gemmobacter, for which the name Gemmobacter tilapiae sp. nov. is proposed. The type strain is Ruye-53(T) ( = BCRC 80261(T)  = KCTC 23310(T)).
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Shih-Yi
SY
Sheu
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Der-Shyan
DS
Sheu
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Fu-Sian
FS
Sheu
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Wen-Ming
WM
Chen
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D006885:Hydroxybutyrates / Q000378:metabolism*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011091:Polyesters / Q000378:metabolism*; D061005:Ponds; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013624:Taiwan; D000094122:Polyhydroxybutyrates
D004269:DNA, Bacterial; D005227:Fatty Acids; D006885:Hydroxybutyrates; D010743:Phospholipids; D011091:Polyesters; D011809:Quinones; D012336:RNA, Ribosomal, 16S; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates