Improved detection and identification of aquatic fungi and chitin in aquatic environments.
Chitin is a polymer of major importance in aquatic environments. We report on a new chitin-staining method for environmental samples. Major advantages of this method are (i) its high specificity and (ii) its suitability for combination with general stains, such as 4',6-diamidino-2-phenylindole (DAPI), or taxa-specific methods, such as fluorescence in situ hybridization (FISH). This method lets aquatic mycologists explore basal fungal lineages directly in situ, identify their hosts, occurrence and evolutionary traits.
Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Dept. Limnology of Stratified Lakes, Stechlin, Germany.
Christian
C
Wurzbacher
Hans-Peter
HP
Grossart
D018441:Biofilms; D002473:Cell Wall / Q000648:ultrastructure; D002686:Chitin / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D005658:Fungi / Q000302:isolation & purification* / Q000648:ultrastructure; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007211:Indoles; D008856:Microscopy, Fluorescence; D012680:Sensitivity and Specificity; D058432:Sporangia / Q000648:ultrastructure; D015003:Yeasts / Q000648:ultrastructure
D005456:Fluorescent Dyes; D007211:Indoles; D002686:Chitin; C007293:DAPI