Down-regulation of A20 mRNA expression in peripheral blood mononuclear cells from patients with systemic lupus erythematosus.
OBJECTIVE
A20 gene functions in negative immunoregulation and its SNP is related to SLE disease. But its expression level in immune cells from SLE patients is still unclear. The aim of this study is to investigate whether the expression of A20 is associated with pathogenesis of SLE.


METHODS
Real-time transcription-polymerase chain reaction analysis (RT-PCR) was used to determine expression of A20 mRNA in peripheral blood mononuclear cells (PBMC) from 37 patients with SLE and 32 healthy controls.


RESULTS
A20 expression was decreased in SLE patients compared with healthy controls (p = 0.0133). The expression level of A20 gene negatively correlated with the SLE disease activity index (SLEDAI) (r =-0.4661, p = 0.0036) and erythrocyte sedimentation rate (ESR) (r =-0.5222, p = 0.0009). Furthermore, SLE patients with nephritis had a lower expression of A20 than those without nephritis (p = 0.0188).


CONCLUSIONS
Our results suggest that the insufficient expression of A20 gene in PBMC may take part in the pathogenesis of SLE disease.
Institute of Immunology, School of Medicine, Shandong University, 44# Wenhua Xi Road, Jinan, 250012, China.
Di
D
Li
Lei
L
Wang
Yuchen
Y
Fan
Lijun
L
Song
Chun
C
Guo
Faliang
F
Zhu
Lining
L
Zhang
Yongyu
Y
Shi
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D004268:DNA-Binding Proteins / Q000097:blood / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000378:metabolism* / Q000473:pathology; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000235:genetics / Q000473:pathology; D008297:Male; D009687:Nuclear Proteins / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index; D015398:Signal Transduction; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3
D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C076529:TNFAIP3 protein, human; D000072598:Tumor Necrosis Factor alpha-Induced Protein 3