Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS).
We have combined the benefits of a TaqMan assay with surface enhanced Raman scattering (SERS), to generate a novel DNA detection method which provides increased sensitivity, with clear applications for disease identification through clinical testing. Target DNA detection limits by SERS were shown to be lower than conventional fluorescence detection and clinically relevant samples of methicillin-resistant Staphylococcus aureus were detected with high specificity.
Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral St, Glasgow, G1 1XL, UK.
Mhairi M
MM
Harper
Barry
B
Robertson
Alastair
A
Ricketts
Karen
K
Faulds
D004247:DNA / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis; D057230:Limit of Detection; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics; D016133:Polymerase Chain Reaction; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D019914:Taq Polymerase / Q000378:metabolism*
D004269:DNA, Bacterial; D004247:DNA; D019914:Taq Polymerase