Complete sequence of the suicide vector pJP5603.
We have sequenced the complete R6K-based and mobilizable suicide vector pJP5603. For the replication of the vector a trans supply of the pir-encoded π protein of plasmid R6K is essential. The 3.126 kb plasmid encodes a kanamycin resistance cassette for selection and contains a lacZ-α-system that allows a blue-white selection of cloned fragments.
Helmholtz-Centre for Infection Research, Group Microbial Communication, Braunschweig, Germany. tri07@helmholtz-hzi.de
Thomas
T
Riedel
Meike
M
Rohlfs
Ina
I
Buchholz
Irene
I
Wagner-Döbler
Michael
M
Reck
D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D059765:Homologous Recombination; D007612:Kanamycin / Q000378:metabolism; D007763:Lac Operon; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics
D001426:Bacterial Proteins; D007612:Kanamycin