A Theiler's virus BeAn strain shows a persistent profile of BHK-21 cell infection as determined by genome detection using real-time RT-PCR and expression of L* protein.
Cells from various tissues and species are able to bind to Theiler's virus strain DA and allow it to replicate to some extent. Meanwhile, permissiveness in vitro to BeAn strains has not been well investigated. In this paper, the BeAn 8386 virus was subjected to five passages in BHK-21 cells and showed a persistent profile. In order to follow the in vitro infection, real-time RT-PCR to detect the IRES, L* and 3A3B regions of the Theiler's virus genome was carried out in the first and last passages. In addition, the expression of L* protein was detected. These findings confirm the persistence of the virus in vitro, even in the absence of cytopathic effect (CPE).
Laboratório de Febre amarela, Bio-Manguinhos, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
Alvaro Jorge
AJ
Velloso
Haroldo Cid
HC
da Silva Junior
Eneida Almeida
EA
Santos
Eliane Barbosa
EB
Baroni
Marcia Terezinha Baroni
MT
de Moraes
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D014644:Genetic Variation; D016679:Genome, Viral; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017945:Theilovirus / Q000145:classification* / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
D014764:Viral Proteins