Olleya namhaensis sp. nov., isolated from wood falls, and emended description of the genus Olleya Mancuso Nichols et al. 2005 emend. Lee et al. 2010.
A Gram-stain-negative, non-flagellated, gliding, rod-shaped bacterial strain, designated WT-MY15(T), was isolated from wood falls in the South Sea in Korea and subjected to a polyphasic taxonomic study. Strain WT-MY15(T) grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain WT-MY15(T) clustered with the type strains of two Olleya species, exhibiting 16S rRNA gene sequence similarity values of 97.7-98.1 %. Strain WT-MY15(T) contained MK-6 as the predominant menaquinone. The fatty acid and polar lipid profiles of strain WT-MY15(T) were similar to those of Olleya aquimaris L-4(T) and Olleya marilimosa CIP 108537(T). The DNA G+C content of strain WT-MY15(T) was 42.8 mol% and its mean DNA-DNA relatedness values with O. aquimaris L-4(T) and O. marilimosa CIP 108537(T) were 8.3 and 5.6 %, respectively. The phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain WT-MY15(T) is separate from the two recognized species of the genus Olleya. On the basis of the data presented, strain WT-MY15(T) is considered to represent a novel species of the genus Olleya, for which the name Olleya namhaensis sp. nov. is proposed. The type strain is WT-MY15(T) ( = KCTC 23673(T) = CCUG 61507(T)). An emended description of the genus Olleya is also presented.
University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, Republic of Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Mi-Hwa
MH
Lee
University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, Republic of Korea.|Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Yong-Taek
YT
Jung
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Sooyeon
S
Park
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Jung-Hoon
JH
Yoon
D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis; D014934:Wood / Q000382:microbiology*
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6