[Phylogenetic relationships of honey bees (Hymenoptera: Apinae) inferred from mitochondrial and nuclear DNA sequences].
The sequences of mitochondrial ND2, CO2, and 16S rRNA genes and nuclear ITPR gene were obtained from 22 samples of 5 Apis species from China. The characteristics of the sequences and the pairwise distances among species were analyzed. Phylogenetic trees were reconstructed for Apis species using maximum parsimony, neighbor-joining and maximum likelihood methods together with the sequences of the other 4 Apis species downloaded from GenBank. Results supported that Apis species were divided into three major clusters: dwarf bees (A. florea and A. andreniformis), giant bees (A. dorsata and A. laboriosa), and cavity-nesting bees (A. mellifera, A. cerana, A. koschevnikovi, A. nigrocinta, and A. nuluensis). The dwarf honey bees were confirmed as basal lineage. Our study also revealed a high level of genetic divergence between A. dorsata from Hainan Island and China mainland.
College of Animal Sciences, Zhejiang University, Hangzhou, China. caolianfei1981@163.com
Lian-Fei
LF
Cao
De-Fang
DF
Niu
Shao-Yu
SY
He
Hai-Ou
HO
Kuang
Fu-Liang
FL
Hu
D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics*; D010802:Phylogeny; D013045:Species Specificity
D004272:DNA, Mitochondrial; D019476:Insect Proteins; D024101:Mitochondrial Proteins