Synthesis of a fluorescently tagged sialic acid analogue useful for live-cell imaging.
A cytidine 5'-monophosphate (CMP)-sialic acid analogue carrying a fluorescent reporter group, an inhibitor of sialyltransferase, was synthesised in order to investigate glycan synthesis events in cells. The compound was found to be a substrate of a CMP-sialic acid transporter, and specific Golgi vesicles were visualised in the cells.
RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
Katsuhiko
K
Suzuki
Atsuko
A
Ohtake
Yukishige
Y
Ito
Osamu
O
Kanie
D000818:Animals; D001896:Boron Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D003568:Cytidine Monophosphate / Q000737:chemistry / Q000493:pharmacokinetics*; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D005453:Fluorescence; D008099:Liver / Q000737:chemistry* / Q000166:cytology; D018613:Microscopy, Confocal; D015394:Molecular Structure; D019158:N-Acetylneuraminic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000493:pharmacokinetics*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D012799:Sialyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism; D013329:Structure-Activity Relationship
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004791:Enzyme Inhibitors; D012799:Sialyltransferases; D003568:Cytidine Monophosphate; D019158:N-Acetylneuraminic Acid