[Dynamic change of IL-10 and TGF-beta1 in the liver of Echinococcus multilocularis-infected mice].
OBJECTIVE
To observe the dynamic expression and function of IL-10 and TGF-beta1 in liver of BALB/c mice infected with Echinococcus multilocularis (Em).


METHODS
Sixty female BALB/c mice were randomly divided into experiment group and control group. Mice in the experiment group were each injected with 0.2 ml Em protoscolex suspension (containing about 400 protoscoleces) , while those in control group received same volume of normal saline. At 2, 8, 30, 90, 180, and 360 d after infection, 5 mice from each group were sacrificed and liver specimens were collected for pathological examination and immunohistochemical detection for IL-10 and TGF-beta1.


RESULTS
In mice of the experiment group, Em cysts in different sizes were found in the abdominal cavity and the liver tissue, which gradually enlarged with the time. HE staining showed infiltration of lymphocytes in liver tissue, pathological change between the cyst wall and hepatic cells. In the control, the liver lobules showed integrity and inflammatory cells were seen occasionally. The level of IL-10 expression in liver tissue of the infected mice increased with the time, and reached a peak [(1639 +/- 1.73) %] at 90 d post-infection and maintained a high level thereafter. The expression of TGF-beta1 also reached the highest level [(23.69 +/- 2.29) %] . Both were significantly higher than the control (P < 0.01), though a low level expression was found in the control at 90d post-injection.


CONCLUSIONS
The expressions of IL-10 and TGF-beta1 both increase in the middle and late stages of the infection. Besides, their inhibited functions do not be helpful for clearing and controlling Echinococcus multilocularis infection in livers.
Medical Research Center and Xinjiang Key Lab of Echinococcosis, Urumqi 830011, China.
Hui
H
Zhao
Xue
X
Bai
Xiao-han
XH
Nie
Jiang-tao
JT
Wang
Xi-xian
XX
Wang
Nan-nan
NN
Pang
Yu-jiao
YJ
Li
Xiu-min
XM
Ma
Hao
H
Wen
Jian-bing
JB
Ding
D000818:Animals; D004443:Echinococcosis / Q000378:metabolism* / Q000469:parasitology; D048210:Echinococcus multilocularis; D005260:Female; D016753:Interleukin-10 / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
C508609:IL10 protein, human; C508961:Tgfb1 protein, mouse; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10