[Reactivation of oxygen accumulation function after complete removal of manganese from the photosystem II particles].
Reactivation of 0² evolution function has been studied in PS-2 particles after complete removal of Mn and water soluble 10, 17, 24, 33 kDa proteins, It has been shown that 0² evolution function in such particles can be reactivated by adding 5 μM Mn² and 20 mM Ca²(+). Preliminary illumination of the sample is necessary to exhibit the reactivation effect of 0² evolution. The maximum value of the reactivation of 0² evolution rate is about 60% of the control. Upon illumination of the reactivated particles with flashes of 1s duration and at a frequency of 0,1 Hz, 0² evolution occurs according to the mechanism analogous to that in the initial parties of PS 2. Thus the reactivation of water oxidation and 0² evolution after complete removal of Mn and water soluble 10, 17, 24, 33 kDa proteins resulting in the suppression of 0² evolution function has been shown for the first time and it can serve as a basic approach for profound investigation of the mechanism of photosynthetic water oxidation.
M A
MA
Shafiev
G M
GM
Anan'ev
S I
SI
Allakhverdiev
T N
TN
Smolova
V V
VV
Klimov
D002122:Calcium Chloride / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D008345:Manganese / Q000378:metabolism*; D017895:Manganese Compounds / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D055668:Photochemical Processes; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D014867:Water / Q000378:metabolism
D002712:Chlorides; D017895:Manganese Compounds; D045332:Photosystem II Protein Complex; D014867:Water; D008345:Manganese; D002122:Calcium Chloride; C025340:manganese chloride; D010100:Oxygen