Solid-phase microextraction combined with comprehensive two-dimensional gas chromatography for fatty acid profiling of cell wall phospholipids.
The combination of solid-phase microextraction (SPME) with comprehensive two-dimensional gas chromatography is evaluated here for fatty acid (FA) profiling of the glycerophospholipid fraction from human buccal mucosal cells. A base-catalyzed derivatization reaction selective for polar lipids such as glycerophospholipid was adopted. SPME is compared to a miniaturized liquid-liquid extraction procedure for the isolation of FA methyl esters produced in the derivatization step. The limits of detection and limits of quantitation were calculated for each sample preparation method. Because of its lower values of limits of detection and quantitation, SPME was adopted. The extracted analytes were separated, detected, and quantified by comprehensive two-dimensional gas chromatography with flame ionization detection (FID). The combination of SPME and comprehensive two-dimensional gas chromatography with FID, using a selective derivatization reaction in the preliminary steps, proved to be a simple and fast procedure for FA profiling, and was successfully applied to the analysis of adult human buccal mucosal cells.
Institute of Chemistry, University of Campinas (Unicamp), Campinas, SP, Brazil.
Stanislau
S
Bogusz
Leandro Wang
LW
Hantao
Soraia Cristina Gonzaga Neves
SC
Braga
Valtenice de Cássia Rodrigues
Vde C
de Matos França
Marcelo Fernandes
MF
da Costa
Russell David
RD
Hamer
Dora Fix
DF
Ventura
Fabio
F
Augusto
D002473:Cell Wall / Q000737:chemistry*; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis*; D020404:Glycerophospholipids / Q000737:chemistry*; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology*; D052617:Solid Phase Microextraction
D005227:Fatty Acids; D020404:Glycerophospholipids