The inhibitory activity of typified propolis against Enterococcus species.
Propolis, a natural bee product widely used for its antimicrobial activity, was tested against isolates of Enterococcus from humans, pig-tailed macaques, isolates of refractory endodontic treatment cases, and isolates from Lactobacillus-containing food supplements. Typification of the propolis was performed by high-performance liquid chromatography (HPLC) by which prenylated compounds, cinnamic acid derivatives, and flavonoids were detected as the main constituents. Minimum inhibitory concentrations (MIC) were determined using the agar dilution method. All human and animal Enterococcus isolates demonstrated MIC values of 1600 microg/mL. Enterococcal species of human and animal origin were inhibited by propolis. Particularly, human isolates of E. faecium and E. faecalis of refractory endodontic treatment cases were susceptible to propolis of Brazilian origin.
School of Dental Medicine, University of Pittsburgh, Departments of Oral Biology and Endodontics, 3501 Terrace Street, Pittsburgh, PA 15260, USA.
Bernard J
BJ
Moncla
Peter W
PW
Guevara
James A
JA
Wallace
Maria C
MC
Marcucci
Jacques E
JE
Nor
Walter A
WA
Bretz
D002851:Chromatography, High Pressure Liquid; D016983:Enterococcus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D011429:Propolis / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
D011429:Propolis