Follicular fluid protein content (FSH, LH, PG4, E2 and AMH) and polar body aneuploidy.
OBJECTIVE
Our objective was to identify a marker for oocyte aneuploidy in follicular fluid (FF) in women with an increased risk of oocyte aneuploidy after controlled ovarian hyperstimulation.


METHODS
Three groups of oocytes were constituted for polar body screening by FISH (chromosomes 13, 16, 18, 21 and 22): Group 1, advanced maternal age (n = 156); Group 2, implantation failure (i.e. no pregnancy after the transfer of more than 10 embryos; n = 101) and Group 3, implantation failure and advanced maternal age (n = 56). FSH and other proteins were assayed in the corresponding FF samples.


RESULTS
Of the 313 oocytes assessed, 35.78 % were abnormal. We found a significant difference between the follicular FSH levels in normal oocytes and abnormal oocytes (4.85 ± 1.75 IU/L vs. 5.41 ± 2.47 IU/L, respectively; p = 0.021). We found that the greater the number of chromosomal abnormalities per oocyte (between 0 and 3), the higher the follicular FSH level.


CONCLUSIONS
High FF FSH levels were associated with oocyte aneuploidy in women having undergone controlled ovarian hyperstimulation.
Service d'Histologie, Embryologie, Biologie de la Reproduction, Cytogénétique et Génétique Médicale, Centre Hospitalier Poissy St Germain en Laye, ART Center, Poissy and EA2493, Paris Ile de France, Ouest Faculty, Versailles University, 10, rue du Champ Gaillard, 78303 Poissy, France. ibhammoud@yahoo.com
I
I
Hammoud
F
F
Vialard
M
M
Bergere
M
M
Albert
D Molina
DM
Gomes
M
M
Adler
L
L
Malagrida
M
M
Bailly
R
R
Wainer
J
J
Selva
D000328:Adult; D000782:Aneuploidy; D054304:Anti-Mullerian Hormone / Q000032:analysis / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D004958:Estradiol / Q000032:analysis* / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis* / Q000378:metabolism; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007986:Luteinizing Hormone / Q000032:analysis* / Q000378:metabolism; D008297:Male; D008423:Maternal Age; D009865:Oocytes / Q000502:physiology*; D059705:Polar Bodies / Q000502:physiology*; D011247:Pregnancy; D019836:Preimplantation Diagnosis / Q000379:methods*; D020554:Sperm Injections, Intracytoplasmic; D016896:Treatment Outcome
D015415:Biomarkers; D004958:Estradiol; D054304:Anti-Mullerian Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone