title: Improvement of bacterial hydrogen production by ATP in mixed organic compounds extracted from Rhodobacter sphaeroides aerobically cultured under dark conditions.
abstract: The aim of this study was to increase the hydrogen production of recombinant Escherichia coli harboring HupSL hydrogenase by supplementing physiologically activating compounds extracted from Rhodobacter sphaeroides cultured under anaerobic dark condition after treating them with dimethyl sulfoxide, and the 0.5% extracts contained 4×10(-8)M ATP, which was 100-fold higher than that in the extracts from E. coli. In addition, it was found that the hydrogen production from recombinant E. coli harboring HupSL hydrogenase isolated from R. sphaeroides was doubled under anaerobic conditions when it was supplemented by the extracts from R. sphaeroides cultured aerobically in dark conditions, and this also showed consistent pattern with the increased level of HupSL hydrogenase expression. Therefore, we conclude that the mixed organic compounds extracted from R. sphaeroides have an ATP which enhances the hydrogen production by increasing the amount of HupSL hydrogenase.
authors.affiliation: Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Deokjin-Dong, 1Ga Deokjin-Gu, Jeonju 561-756, South Korea.
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Am
authors.initials: A
authors.lastname: Jang
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Park
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: Bong-Woo
authors.initials: BW
authors.lastname: Chung
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000332:Aerobiosis; D000693:Anaerobiosis; D015153:Blotting, Western; D003624:Darkness; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism; D009930:Organic Chemicals / Q000302:isolation & purification*; D011995:Recombination, Genetic / Q000235:genetics; D012242:Rhodobacter sphaeroides / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D009930:Organic Chemicals; D006859:Hydrogen; D000255:Adenosine Triphosphate; D006864:Hydrogenase