title: Characterization of the tissue-level Ca2+ signals in spontaneously contracting human myometrium.
abstract: In the labouring uterus, millions of myocytes forming the complex geometrical structure of myometrium contract in synchrony to increase intrauterine pressure, dilate the cervix and eventually expel the foetus through the birth canal. The mechanisms underlying the precise coordination of contractions in human myometrium are not completely understood. In the present study, we have characterized the spatio-temporal properties of tissue-level [Ca(2+)](i) transients in thin slices of intact human myometrium. We found that the waveform of [Ca(2+)](i) transients and isotonic contractions recorded from thin slices was similar to the waveform of isometric contractions recorded from the larger strips in traditional organ bath experiments, suggesting that the spatio-temporal information obtained from thin slices is representative of the whole tissue. By comparing the time course of [Ca(2+)](i) transients in individual cells to that recorded from the bundles of myocytes we found that the majority of myocytes produce rapidly propagating long-lasting [Ca(2+)](i) transients accompanied by contractions. We also found a small number of cells showing desynchronized [Ca(2+)](i) oscillations that did not trigger contractions. The [Ca(2+)](i) oscillations in these cells were insensitive to nifedipine, but readily inhibited by the T-type Ca(2+) channel inhibitor NNC55-0396. In conclusion, our data suggest that the spread of [Ca(2+)](i) signals in human myometrium is achieved via propagation of long-lasting action potentials. The propagation was fast when action potentials propagated along bundles of myocytes and slower when propagating between the bundles of uterine myocytes.
authors.affiliation: Warwick Medical School, University of Warwick, Coventry, UK.
authors.forename: Gilles
authors.initials: G
authors.lastname: Bru-Mercier
authors.forename: Joanna E
authors.initials: JE
authors.lastname: Gullam
authors.forename: Steven
authors.initials: S
authors.lastname: Thornton
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Blanks
authors.forename: Anatoly
authors.initials: A
authors.lastname: Shmygol
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects; D005260:Female; D006801:Humans; D007537:Isometric Contraction / Q000187:drug effects; D032342:Muscle Cells / Q000502:physiology; D009119:Muscle Contraction; D009215:Myometrium / Q000166:cytology / Q000502:physiology*; D009543:Nifedipine / Q000494:pharmacology; D011247:Pregnancy; D014590:Uterine Contraction
chemical_list: D002121:Calcium Channel Blockers; D009543:Nifedipine; D002118:Calcium