title: Solvent-induced tuning of internal structure in a protein amyloid protofibril.
abstract: An important goal in studies of protein aggregation is to obtain an understanding of the structural diversity that is characteristic of amyloid fibril and protofibril structures at the molecular level. In this study, what to our knowledge are novel assays based on time-resolved fluorescence anisotropy decay and dynamic quenching measurements of a fluorophore placed at different specific locations in the primary structure of a small protein, barstar, have been used to determine the extent to which the protein sequence participates in the structural core of protofibrils. The fluorescence measurements reveal the structural basis of how modulating solvent polarity results in the tuning of the protofibril conformation from a pair of parallel β-sheets in heat-induced protofibrils to a single parallel β-sheet in trifluorethanol-induced protofibrils. In trifluorethanol-induced protofibrils, the single β-sheet is shown to be built up from in-register β-strands formed by nearly the entire protein sequence, while in heat-induced protofibrils, the pair of β-sheets motif is built up from β-strands formed by only the last two-third of the protein sequence.
authors.affiliation: Department of Chemical Sciences, Tata Institute of Fundamental Research, Mumbai, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Jha
authors.forename: Satya
authors.initials: S
authors.lastname: Narayan
authors.forename: Jayant B
authors.initials: JB
authors.lastname: Udgaonkar
authors.forename: G
authors.initials: G
authors.lastname: Krishnamoorthy
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D005454:Fluorescence Polarization; D006358:Hot Temperature; D006860:Hydrogen Bonding; D008958:Models, Molecular; D008970:Molecular Weight; D055503:Protein Multimerization / Q000187:drug effects*; D055550:Protein Stability / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D012997:Solvents / Q000494:pharmacology*; D014270:Trifluoroethanol / Q000494:pharmacology
chemical_list: D000682:Amyloid; D001426:Bacterial Proteins; D012997:Solvents; C057438:barstar protein, Bacillus amyloliquefaciens; D014270:Trifluoroethanol