title: Positively charged supported lipid bilayers as a biomimetic platform for neuronal cell culture.
abstract: The supported lipid bilayer (SLB) is a well-known system for studying the cell membrane and membrane proteins. It is also promising as a platform for studying cell processes: the cell adhesion, the cell membrane receptors, and the intercellular signaling processes. SLBs made of natural lipids appeared to be protein and cell repellent. Thus, to use the SLB as a substrate for cells, one should functionalize them to provide adhesion. In the present paper, we describe a simple approach to promote adhesion of neuronal cells to the SLB without using proteins or peptides, by introducing positively charged lipids 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) into the SLB made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). We show that neurons adhere to these bilayers and grow for at least 10 days. The SLBs themselves were found to degrade with time in cell culture conditions, but maintained fluidity (as revealed by fluorescence recovery after photobleaching), demonstrating the possibility of using SLBs for studying neuronal cells in culture.
authors.affiliation: Peter Grünberg Institute/Institute of Complex Systems Bioelectronics (PGI-8/ICS-8), Research Center Juelich, D-52425 Juelich, Germany.
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Afanasenkau
authors.forename: Andreas
authors.initials: A
authors.lastname: Offenhäusser
mesh_terms: D000818:Animals; D032701:Biomimetics; D002448:Cell Adhesion; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008954:Models, Biological; D015394:Molecular Structure; D009474:Neurons / Q000166:cytology*; D010713:Phosphatidylcholines / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D051381:Rats
chemical_list: D005229:Fatty Acids, Monounsaturated; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D000644:Quaternary Ammonium Compounds; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane; C028694:1-palmitoyl-2-oleoylphosphatidylcholine