title: Studies on the characteristic and activity of low-molecular fragments from zymosan.
abstract: Zymosan was hydrolysed with HCl and fractionated by ultrafiltration and dialysis to obtain water-soluble fragments A, B and C. Physical and chemical analyses showed that these fractions are composed primarily of glucose and have molecular weights of 8 kDa, 5 kDa and 2 kDa, respectively. A glycosidic linkage analysis indicated that they are mainly composed of β-1,3-glucans. Fragment A, which has the highest molecular weight, contains approximately 30% β-1,6-linked glucans, but fragment C is almost entirely composed of linear β-1,3-glucan chains. The anti-chronic atrophic gastritis activity experiments showed that fragment A has significant activity, the activity of zymosan is quite low and the activities of fragments B and C are in between those of fragment A and zymosan.
authors.affiliation: Changchun University of Chinese Traditional Medicine, Changchun 130117, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Ruizhi
authors.initials: R
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Qie
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Chen
authors.forename: Duoduo
authors.initials: D
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Qipin
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry*; D002850:Chromatography, Gel; D002908:Chronic Disease; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000378:metabolism; D005757:Gastritis, Atrophic / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007114:Immunization; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008970:Molecular Weight; D051381:Rats; D017208:Rats, Wistar; D013552:Swine; D015054:Zymosan / Q000737:chemistry / Q000378:metabolism*; D047071:beta-Glucans / Q000737:chemistry / Q000378:metabolism*
chemical_list: D047071:beta-Glucans; D015054:Zymosan; C033363:beta-1,3-glucan