title: Increased hydrogen production in co-culture of Chlamydomonas reinhardtii and Bradyrhizobium japonicum.
abstract: Co-cultivation of Bradyrhizobium japonicum with Chlamydomonas reinhardtii strain cc849 or the transgenic strain lba, which was hetero-expressed the gene of the soybean leghemoglobin apoprotein Lba in chloroplasts of the strain cc849, in Tris-acetate-phosphate (TAP) or TAP-sulfur free media, improved H(2) yield. H(2) production was 14 times and growth was 26% higher when strain lba and B. japonicum were co-cultured, as compared with cultivation of the algal strain alone under the same conditions. The increase in respiration rate or fast O(2) consumption by about 8 times in the co-cultures was the major reason for the improvement.
authors.affiliation: The CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, No.7 Beitucheng West Road, Chaoyang District, Beijing 100029, PR China. wushx@big.ac.cn
authors.forename: Shuangxiu
authors.initials: S
authors.lastname: Wu
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.forename: Quanxi
authors.initials: Q
authors.lastname: Wang
mesh_terms: D020369:Bradyrhizobium / Q000378:metabolism*; D019069:Cell Respiration; D016825:Chlamydomonas reinhardtii / Q000254:growth & development / Q000378:metabolism*; D018920:Coculture Techniques / Q000379:methods*; D006859:Hydrogen / Q000378:metabolism*; D010101:Oxygen Consumption
chemical_list: D006859:Hydrogen