title: Birth of common marmoset (Callithrix jacchus) offspring derived from in vitro-matured oocytes in chemically defined medium.
abstract: Optimization of oocyte culture conditions is a crucial aspect of reproductive biology and technology. In the present study, maturation of germinal vesicle-stage marmoset oocytes were evaluated in the following media: Waymouth medium, Waymouth medium containing porcine follicular fluid (pFF) (Waymouth-pFF medium), and porcine oocyte medium (POM). Oocytes cultured in Waymouth-pFF medium had higher maturation rates to the metaphase II stage than those cultured in Waymouth medium (36.1% vs. 24.8%, respectively, P < 0.05), indicating the suitability of this medium for culturing marmoset oocytes. Hence, maturation of marmoset oocytes cultured in POM was subsequently evaluated. The rate of maturation to the metaphase I stage was significantly higher and degradation rates were significantly lower in oocytes cultured in POM than those cultured in Waymouth medium. In addition, three offspring were successfully obtained after transfer of embryos matured in chemically defined medium. Therefore, we concluded that POM was suitable for marmoset oocyte culture. Furthermore, this was apparently the first report of marmoset offspring derived from oocytes cultured in chemically defined medium.
authors.affiliation: Department of Applied Developmental Biology, Central Institute for Experimental Animals, Kawasaki, Kanagawa, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Tomioka
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
authors.forename: A
authors.initials: A
authors.lastname: Shimada
authors.forename: K
authors.initials: K
authors.lastname: Yoshioka
authors.forename: E
authors.initials: E
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D002144:Callithrix / Q000196:embryology*; D018929:Cell Culture Techniques / Q000662:veterinary*; D003470:Culture Media; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D015571:Follicular Fluid / Q000502:physiology; D008297:Male; D008677:Metaphase; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000662:veterinary*; D027724:Reproductive Techniques, Assisted / Q000662:veterinary*; D013552:Swine
chemical_list: D003470:Culture Media