title: Cytokine profiles of patients with enterohemorrhagic Escherichia coli O111-induced hemolytic-uremic syndrome.
abstract: Proinflammatory cytokines are related to the pathogenesis of enterohemorrhagic Escherichia coli (EHEC) infection and hemolytic-uremic syndrome (HUS). We assessed the kinetics of the release of cytokines such as neopterin, interleukin (IL)-6, IL-8 and tumour necrosis factor (TNF)-α and the soluble forms of type I and II TNF receptors during EHEC O111-induced HUS (EHEC O111/HUS). Fourteen patients with EHEC O111/HUS were enrolled in this study. Serum concentrations of all cytokines other than TNF-α were significantly elevated in patients with severe HUS compared with those in patients with mild HUS. Although serum concentrations of TNF-α were not significantly higher in patients with severe HUS, most patients with acute encephalopathy showed elevated TNF-α levels. Serum concentrations of these cytokines rapidly and markedly increased, and massive hypercytokinaemia developed 1 day before the diagnosis of HUS in patients with severe HUS. Changes in the number of white blood cells and concentration of serum lactate dehydrogenase were significantly larger between the onset of hemorrhagic colitis and the time of the diagnosis of HUS in patients with severe HUS compared with those in patients with mild HUS. Proinflammatory cytokines play an important role in the pathogenesis of EHEC infection and development of severe complications, including HUS and encephalopathy. Monitoring the cytokine profile may be useful for assessing disease activity of EHEC O111 infections.
authors.affiliation: Department of Pediatrics, School of Medicine, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Japan. shimizum@staff.kanazawa-u.ac.jp
authors.forename: Masaki
authors.initials: M
authors.lastname: Shimizu
authors.forename: Mondo
authors.initials: M
authors.lastname: Kuroda
authors.forename: Natsumi
authors.initials: N
authors.lastname: Sakashita
authors.forename: Michio
authors.initials: M
authors.lastname: Konishi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kaneda
authors.forename: Noboru
authors.initials: N
authors.lastname: Igarashi
authors.forename: Junya
authors.initials: J
authors.lastname: Yamahana
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Taneichi
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kanegane
authors.forename: Mika
authors.initials: M
authors.lastname: Ito
authors.forename: Shigeru
authors.initials: S
authors.lastname: Saito
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Ohta
authors.forename: Takumi
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Kengo
authors.initials: K
authors.lastname: Furuichi
authors.forename: Takashi
authors.initials: T
authors.lastname: Wada
authors.forename: Masaru
authors.initials: M
authors.lastname: Nakagawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yokoyama
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yachie
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D016207:Cytokines / Q000097:blood*; D054324:Enterohemorrhagic Escherichia coli; D004927:Escherichia coli Infections / Q000150:complications* / Q000382:microbiology; D005260:Female; D006463:Hemolytic-Uremic Syndrome / Q000097:blood* / Q000209:etiology* / Q000382:microbiology; D006801:Humans; D007223:Infant; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D019798:Neopterin / Q000097:blood; D018124:Receptors, Tumor Necrosis Factor / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D018124:Receptors, Tumor Necrosis Factor; D014409:Tumor Necrosis Factor-alpha; D019798:Neopterin; D007770:L-Lactate Dehydrogenase