title: Association between polymorphisms of the E-selectin gene, hepatitis             B virus DNA copies and preS1 antigen in patients with chronic hepatitis B infection.
abstract: The aim of this study was to investigate the relationship between E-selectin             +G98T, +A561C polymorphisms and the levels of hepatitis B virus (HBV) DNA and             preS1 antigen (preS1Ag) in patients with chronic hepatitis B (CHB) infection.             Polymorphisms of the E-selectin gene in 150 CHB patients and 150 healthy controls             of two different nationalities were detected using polymerase chain reaction-restriction             fragment length polymorphism (PCR-RFLP). Real-time quantitative PCR was used to             detect the levels of HBV DNA. preS1Ag and five items of hepatitis B were detected             by enzyme-linked immunosorbent assay. Two genotypes, GG (94%, 96%) and GT (6%,             4%) of the E-selectin +G98T polymorphism, and AA (78.67%, 80.67%) and AC (21.33%,             19.33%) of the +A561C polymorphism, were found in these patients. There were also             significant differences in the two nationalities in the genotypic frequencies             in +A561C polymorphisms between patients and healthy subjects (χ2=5.489, χ2=5.653;             P<0.05). In the patients studied, the relative risk of suffering from CHB in             genotype AC was 2.122 and 2.313-fold higher for the two nationalities, respectively,             than that of the AA genotype (OR=2.122, 95% CI 1.121-4.019; OR=2.313, 95% CI 1.002-5.360).             There was also significant over-representation in the C allele frequency between             the two groups (χ2=5.000, χ2=5.30; P<0.05), and the levels of HBV DNA and preS1Ag             in the AC genotype patients were higher than those in the AA genotype (P<0.01             and P<0.05). E-selectin +A561C and +G98T polymorphisms were present in the             populations studied. Therefore, there is a correlation between E-selectin +A561C             polymorphisms and CHB. Allele C may be one of the predisposing factors, and mutation             of this locus may impact the virus copy number.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, PR China.
authors.forename: Wei Juan
authors.initials: WJ
authors.lastname: Cai
authors.forename: Liang
authors.initials: L
authors.lastname: Yin
authors.forename: Di
authors.initials: D
authors.lastname: Zhou
authors.forename: Wen Jiang
authors.initials: WJ
authors.lastname: Cao
authors.forename: Wei Wei
authors.initials: WW
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Sheng
authors.forename: Jiang
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis*; D019040:E-Selectin / Q000235:genetics*; D018628:Gene Dosage; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000821:virology; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011498:Protein Precursors / Q000378:metabolism*
chemical_list: D004279:DNA, Viral; D019040:E-Selectin; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; C050806:presurface protein 1, hepatitis B surface antigen