title: Effect of DHA+EPA on oxidative stress and apoptosis induced by ischemia-reperfusion in rat kidneys.
abstract: Apoptosis, as well as necrosis, has an important role in post-ischemic renal pathology. The effect of pretreatment with Docosahexaenoic acid+Eicosapentaenoic acid (DHA+EPA) on renal injury and apoptotic protein expression was evaluated. Right nephrectomy was completed on male Wistar rats (255-300 g). The rats received DHA+EPA (200 mg/kg/day) of distilled water orally for 14 days before ischemia reperfusion (IR) or sham operation. A total of 81 rats were divided into three main groups with 6, 24 and 48 h of post-operation or reperfusion period. Serum creatinine (SCr), BUN, creatinine clearance (CCr) and fractional excretion of sodium (FEN a ) were measured. Tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) activities, Bax and Bcl-2 protein expressions and renal histological injury were determined. SCr, BUN and FEN a increased 6-48 h of reperfusion (P < 0.01). Tissue MDA content and Bax expression increased (P < 0.01) and CAT and SOD activities decreased (P < 0.05) in the IR group. DHA+EPA decreased SCr and BUN, FEN a , tissue MDA levels (P < 0.05 vs. IR) and increased CAT and SOD activities and Bcl-2 expression (P < 0.05 vs. IR) for 6-48 h after ischemia. IR induced mild (6 h, P < 0.05) and severe (24-48 h, P < 0.01) tissue damage. Mild-to-moderate tissue damage was observed in DHA+EPA groups from 6 to 48 h of reperfusion period (P < 0.05 vs. IR, 24-48 h). In conclusion, the results suggest that pre-ischemic exposure to DHA+EPA could improve the outcome of early graft function by inhibition of IR-induced oxidative stress and apoptosis.
authors.affiliation: Faculty of Nutrition Sciences & Food Technology, Shahid Beheshti University of Medical Sciences & Health Services, 19395-4741, Tehran, Iran.
authors.forename: Marjan
authors.initials: M
authors.lastname: Ajami
authors.forename: Sayed Hossein
authors.initials: SH
authors.lastname: Davoodi
authors.forename: Rouhollah
authors.initials: R
authors.lastname: Habibey
authors.forename: Nasim
authors.initials: N
authors.lastname: Namazi
authors.forename: Mansoureh
authors.initials: M
authors.lastname: Soleimani
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Pazoki-Toroudi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D007668:Kidney / Q000098:blood supply / Q000187:drug effects / Q000473:pathology; D007677:Kidney Function Tests; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
chemical_list: D004281:Docosahexaenoic Acids; D008315:Malondialdehyde; D015118:Eicosapentaenoic Acid; D002374:Catalase; D013482:Superoxide Dismutase