title: Immobilization of cell-binding peptides on poly-ε-caprolactone film surface to biomimic the peripheral nervous system.
abstract: Cell-material interactions are crucial for cell adhesion and proliferation on biomaterial surfaces. Immobilization of biomolecules leads to the formation of biomimetic substrates, improving cell response. We introduced RGD (Arg-Gly-Asp) sequences on poly-ε-caprolactone (PCL) film surfaces using thiol chemistry to enhance Schwann cell (SC) response. XPS elemental analysis indicated an estimate of 2-3% peptide functionalization on the PCL surface, comparable with carbodiimide chemistry. Contact angle was not remarkably reduced; hence, cell response was only affected by chemical cues on the film surface. Adhesion and proliferation of Schwann cells were enhanced after PCL modification. Particularly, RGD immobilization increased cell attachment up to 40% after 6 h of culture. It was demonstrated that SC morphology changed from round to very elongated shape when surface modification was carried out, with an increase in the length of cellular processes up to 50% after 5 days of culture. Finally RGD immobilization triggered the formation of focal adhesion related to higher cell spreading. In summary, this study provides a method for immobilization of biomolecules on PCL films to be used in peripheral nerve repair, as demonstrated by the enhanced response of Schwann cells.
authors.affiliation: Blond McIndoe Laboratories, School of Biomedicine, The University of Manchester, Manchester Academic Health Science Centre, Oxford Road, Manchester M13 9PT, United Kingdom.
authors.forename: A C
authors.initials: AC
authors.lastname: de Luca
authors.forename: J S
authors.initials: JS
authors.lastname: Stevens
authors.forename: S L M
authors.initials: SL
authors.lastname: Schroeder
authors.forename: J-B
authors.initials: JB
authors.lastname: Guilbaud
authors.forename: A
authors.initials: A
authors.lastname: Saiani
authors.forename: S
authors.initials: S
authors.lastname: Downes
authors.forename: G
authors.initials: G
authors.lastname: Terenghi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism; D055767:Immobilized Proteins / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology*; D017933:Peripheral Nervous System / Q000166:cytology / Q000187:drug effects*; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D012997:Solvents; D013499:Surface Properties; D014835:Volatilization
chemical_list: D001672:Biocompatible Materials; D055767:Immobilized Proteins; D009842:Oligopeptides; D011091:Polyesters; D012997:Solvents; C016240:polycaprolactone; C047981:arginyl-glycyl-aspartic acid