title: In vitro N-acetyl-L-cysteine promotes proliferation and suppresses interleukin-8 expression in adipose-derived stem cells.
abstract: Adipose-derived stem cells (ADSCs) hold great promise for repair and regeneration of burn wounds by producing growth factors, but proinflammatory cytokines such as interleukin-8 (IL-8) released by ADSCs would potentially deepen the wound and inhibit healing. The reported research aimed to identify the effects of N-acetyl-L-cysteine (NAC) on the proliferation, death, and IL-8 production of ADSCs. In the presence or absence of NAC, ADSC proliferation was examined using a CCK-8 Kit, and cell death was evaluated by flow cytometry analysis. Subsequently, IL-8 mRNA expression was detected by reverse transcriptase-polymerase chain reaction and protein production by enzyme-linked immunoassay. Findings showed that cell proliferation in the NAC-treated group was a significant 1.53-fold greater than in the control group, that the apoptosis rate of ADSCs decreased by 55.4 % compared with the control group, and that the necrosis rate decreased by 48.8 %. Additionally, the IL-8 mRNA expression decreased to 46.2 ± 8.7 % that of the control group, and the IL-8 protein production decreased to 9.98 ± 0.57 %. The authors believe that NAC might be helpful in burn wound repair and regeneration by stimulating the proliferation of ADSCs, inhibiting cell death, and suppressing IL-8 production.
  
  
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authors.affiliation: Department of Plastic and Reconstructive Surgery, Union Hospital, Huazhong Science and Technique University, Hubei, China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Xiong
authors.forename: Jiaming
authors.initials: J
authors.lastname: Sun
authors.forename: Christoph
authors.initials: C
authors.lastname: Hirche
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Xia
authors.forename: Marcus
authors.initials: M
authors.lastname: Lehnhardt
authors.forename: Rongrong
authors.initials: R
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Fu
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000273:Adipose Tissue / Q000166:cytology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects*
chemical_list: D016209:Interleukin-8; D000111:Acetylcysteine