title: [Effects of hyperbaric oxygenation combined with As(2)O(3) on proliferation of K562 cells and associated mechanism].
abstract: This study was aimed to explore the effects of hyperbaric oxygenation (HBO) alone or combined with As(2)O(3) on proliferation, apoptosis and expression of HIF-1a, VEGF, caspase-3 mRNA of K562 cells, and the molecular mechanism of As(2)O(3) enhancing the anti-leukemic effect of HBO so as to provide a scientific basis for clinical treatment of chronic myeloid leukemia. The effects of drugs on proliferation of K562 cells was assayed by MTT method, the apoptosis rate of K562 cells was detected by flow cytometry with Annexin V/PI double staining, the expressions of HIF-1a, VEGF, caspase-3 mRNA of K562 cells were determined by real-time quantitative PCR. The results showed that as compared with As(2)O(3) alone, HBO combined with As(2)O(3) could increase inhibitory rate of K562 cell proliferation, and enhance apoptotic effect, obviously down-regulate expressions of HIF-1a and VEGF mRNA, up-regulate expression of caspase-3 mRNA. The effect of HBO combined with As(2)O(3) was higher then effect of As(2)O(3) alone, and their effects were synergistic (P < 0.05). It is concluded that HBO combined with As(2)O(3) can increase the expression of caspase 3 mRNA and decrease the expression of HIF-1a and VEGF mRNA, which may be one of molecular mechanisms underlying their synergistic antileukemia efficacy.
authors.affiliation: Department of Hematology, Qingdao Municipal Hospital, Shandong Province, China.
authors.forename: Song-Mei
authors.initials: SM
authors.lastname: Wang
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Wu
authors.forename: Jun-Qian
authors.initials: JQ
authors.lastname: Huang
authors.forename: Lu-Mei
authors.initials: LM
authors.lastname: Hao
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Wei
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001152:Arsenicals / Q000494:pharmacology*; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D006931:Hyperbaric Oxygenation; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D020014:K562 Cells; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D001152:Arsenicals; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C505407:CASP3 protein, human; D053148:Caspase 3