title: Expression of sheep interleukin 23 (IL23A, alpha subunit p19) in two distinct gastrointestinal diseases.
abstract: This paper reports the sequence of sheep interleukin 23A (p19), and shows that it shares 98% identity with bovine IL23A, 85% with human and 76% with mouse IL23A. It also reports the existence of two allelic variants that differ largely within the region encoding the amino terminal polypeptide signal sequence. An optimized RT-qPCR assay was used to quantify IL23A transcripts in sheep infected with two common gastrointestinal pathogens, the intracellular bacterium Mycobacterium avium subspecies paratuberculosis and the parasitic nematode Teladorsagia circumcincta. No differential expression of IL23A was detected in the mesenteric lymph node of sheep with the different pathogenic forms of paratuberculosis, however significantly high levels of IL23A were detected in the ileal mucosa of the paucibacillary form in comparison with the asymptomatic or multibacillary forms. Similarly, significantly high levels were present in the gastric lymph node draining T. circumcincta-infected abomasum in susceptible sheep. High levels of IL23A seem to be associated with lymphocytic infiltration and inflammation in both diseases but not with the macrophage infiltrate of multibacillary paratuberculosis.
authors.affiliation: The Roslin Institute & R(D)SVS, Easter Bush Veterinary Campus, Roslin, Midlothian EH25 9RG, UK.
authors.forename: Anton G
authors.initials: AG
authors.lastname: Gossner
authors.forename: Virginia M
authors.initials: VM
authors.lastname: Venturina
authors.forename: Anna
authors.initials: A
authors.lastname: Peers
authors.forename: Craig A
authors.initials: CA
authors.lastname: Watkins
authors.forename: John
authors.initials: J
authors.lastname: Hopkins
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D053759:Interleukin-23 / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D016927:Mycobacterium avium subsp. paratuberculosis / Q000235:genetics / Q000276:immunology*; D010283:Paratuberculosis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology; D014251:Trichostrongyloidea / Q000235:genetics / Q000276:immunology*; D014252:Trichostrongyloidiasis / Q000235:genetics / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D053759:Interleukin-23