title: Glutamate-ammonia ligase and reduction of G0 population in PANC-1 cells.
abstract: In our previous study, we screened and isolated genes that were up-regulated after partial pancreatectomy using transcriptomic analysis and glutamate-ammonia ligase (GLUL) was selected for further study based on its effect on differentiation and proliferation. In the immunohistochemical analysis, GLUL was highly up-regulated in the acinar cells and the ductal cells in the pancreas damaged through partial pancreatectomy. Overexpression of GLUL enhanced the proliferation of PANC-1 cells and INS-1 cells. GLUL overexpression shifted the major population of PANC-1 cells from the G0/G1 phase to G2/M phase. In the double thymidine blocking analysis, similar cycle duration was observed between mock cells and GLUL-overexpressing cells while GLUL-overexpressing cells were partially resistant to thymidine blocking. In the FACS analysis of cells stained with Pyronin Y and Hoechst 33342, GLUL-overexpressing cells showed lower population of cells in the G0-quiescent phase than mock cells (5-12%). In addition, GLUL-overexpressing cells had high activation levels of AKT, ERK1/2, JNK, PCNA, c-FOS, and P70S6K in PANC-1 cells. Taken together, these results suggest that GLUL contributes to pancreatic regeneration.
authors.affiliation: College of Medicine, CHA University, CHA General Hospital, Gyeonggi-Do 463-840, Republic of Korea.
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: Key-Hwan
authors.initials: KH
authors.lastname: Lim
authors.forename: Eunmi
authors.initials: E
authors.lastname: Park
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Young-Kil
authors.initials: YK
authors.lastname: Choi
authors.forename: Kwang-Hyun
authors.initials: KH
authors.lastname: Baek
mesh_terms: D061354:Acinar Cells / Q000166:cytology / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D005974:Glutamate-Ammonia Ligase / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D010179:Pancreas / Q000166:cytology / Q000254:growth & development; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D012038:Regeneration; D013936:Thymidine / Q000494:pharmacology
chemical_list: D051057:Proto-Oncogene Proteins c-akt; D005974:Glutamate-Ammonia Ligase; D013936:Thymidine