title: Developmental stage-specific contribution of LGR5(+)  cells to basal and luminal epithelial lineages in the postnatal mammary gland.
abstract: The leucine-rich repeat-containing heterotrimeric guanine nucleotide-binding protein-coupled receptor 5 (LGR5) has been identified as a marker of cycling stem cells in several epithelial tissues, including small intestine, colon, stomach and hair follicle. To investigate whether LGR5 also marks mammary epithelial stem cells, we performed in situ lineage-tracing studies and mammary gland reconstitutions with LGR5-expressing mammary epithelial cells. Interestingly, the LGR5 progeny population in mammary epithelium switches from the luminal to the myoepithelial compartment during the first 12 days of postnatal development, likely reflecting local changes in Wnt signalling. Together, our findings point to a stage-specific contribution of LGR5-expressing cells to luminal and basal epithelial lineages during postnatal mammary gland development.
authors.affiliation: Division of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands. k.d.visser@nki.nl
authors.forename: Karin E
authors.initials: KE
authors.lastname: de Visser
authors.forename: Metamia
authors.initials: M
authors.lastname: Ciampricotti
authors.forename: Ewa M
authors.initials: EM
authors.lastname: Michalak
authors.forename: David Wei-Min
authors.initials: DW
authors.lastname: Tan
authors.forename: Ewoud N
authors.initials: EN
authors.lastname: Speksnijder
authors.forename: Cheei-Sing
authors.initials: CS
authors.lastname: Hau
authors.forename: Hans
authors.initials: H
authors.lastname: Clevers
authors.forename: Nick
authors.initials: N
authors.lastname: Barker
authors.forename: Jos
authors.initials: J
authors.lastname: Jonkers
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology; D005260:Female; D008321:Mammary Glands, Animal / Q000166:cytology / Q000254:growth & development* / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D023421:Models, Animal; D009024:Morphogenesis / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D051153:Wnt Proteins / Q000502:physiology
chemical_list: D015415:Biomarkers; C493597:Lgr5 protein, mouse; D043562:Receptors, G-Protein-Coupled; D051153:Wnt Proteins