title: Phylogenetic analysis of the bovine parainfluenza virus type 3 from cattle herds revealing the existence of a genotype A strain in China.
abstract: In 2009, a bovine parainfluenza virus (BPIV3), named as NM09, was isolated using MDBK cell culture from the nasal swabs of normal cattle in China. The NM09 isolate was characterized by RT-PCR and nucleotide sequence analysis. Its complete genome was 15,456 nucleotides in length. Similar to other sequenced PIV strains, the NM09 virus consisted of six non-overlapping genes, which were predicted to encode nine proteins with conserved and complementary 3' leader and 5' trailer regions, conserved gene starts, gene stops, and trinucleotide intergenic sequences. Nucleotide phylogenetic analysis of matrix and hemagglutinin-neuraminidase gene demonstrated that this NM09 isolate belonged to BPIV3 genotype A instead of the previously reported BPIV3 genotype C in China. It is implicated that the different genotypes A and C might coexist infection for a long time in China.
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animals, Division of Zoonoses, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences (CAAS), 4899 Juye Street, Changchun, Jilin, China. yongjunwen@126.com
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Wen
authors.forename: Xin-Chuan
authors.initials: XC
authors.lastname: Shi
authors.forename: Feng-Xue
authors.initials: FX
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Shu-Qin
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.forename: Ni
authors.initials: N
authors.lastname: Song
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Chen
authors.forename: Shi-Peng
authors.initials: SP
authors.lastname: Cheng
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000821:virology*; D002460:Cell Line; D002681:China; D005814:Genes, Viral; D059646:Genome Size; D005838:Genotype; D015707:HN Protein / Q000235:genetics; D029101:Parainfluenza Virus 3, Bovine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014763:Viral Matrix Proteins / Q000235:genetics; D014776:Virus Cultivation / Q000379:methods
chemical_list: D015707:HN Protein; D014763:Viral Matrix Proteins