title: Molecular identification of a rare subtype of Cryptosporidium hominis in infants in China.
abstract: Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan.
authors.affiliation: The College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
authors.forename: Huili
authors.initials: H
authors.lastname: Zhu
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Zhao
authors.forename: Rongjun
authors.initials: R
authors.lastname: Wang
authors.forename: Longxian
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D002681:China; D003457:Cryptosporidiosis / Q000235:genetics*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000235:genetics; D003967:Diarrhea / Q000235:genetics*; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S