title: Characterization and anti-allergic effect of a polysaccharide from the flower buds of Lonicera japonica.
abstract: A water-soluble polysaccharide (LJP-1), with a molecular weight of 1.8×10(5) Da, was isolated from the flower buds of Lonicera japonica. Gas chromatography (GC) analysis showed that the LJP-1 was mainly composed of d-glucose and a small amount of d-arabinose. On the basis of methylation analysis, LJP-1 had the backbone chain mainly consisting of 1,6-linked Glc and 1,3,6-linked Glc, which was terminated with 1-linked Ara residues at the O-3 position of 1,3,6-linked Glc in a relative molar ratio of 2.9:1:0.9. The anti-allergic effect of LJP-1 was evaluated on allergic contact dermatitis (ACD) induced by picryl chloride (PC) in mouse ear. Similar to prednisolone, orally administrated LJP-1 (20, 40 and 80 mg/kg) potently inhibited the PC-induced ACD, leading to substantial reductions in ear thickness, serum level of IgE and histamine, as well as tissue TNF-α. These results demonstrate that treatment with LJP-1 may be effective for preventing the development of PC-induced ACD.
authors.affiliation: Department of Dermatology, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.forename: Huilian
authors.initials: H
authors.lastname: Che
authors.forename: Da
authors.initials: D
authors.lastname: Ha
authors.forename: Yeping
authors.initials: Y
authors.lastname: Wei
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018926:Anti-Allergic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D002849:Chromatography, Gas; D017449:Dermatitis, Allergic Contact / Q000188:drug therapy* / Q000209:etiology / Q000276:immunology; D004423:Ear / Q000473:pathology; D005260:Female; D035264:Flowers / Q000737:chemistry*; D006632:Histamine / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood; D031239:Lonicera / Q000737:chemistry*; D008745:Methylation; D051379:Mice; D008813:Mice, Inbred ICR; D008970:Molecular Weight; D010853:Picryl Chloride / Q000633:toxicity; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011239:Prednisolone / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D000893:Anti-Inflammatory Agents; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E; D006632:Histamine; D011239:Prednisolone; D010853:Picryl Chloride