title: Analysis of whole genomic expression profiles and screening of the key signaling pathways associated with pancreatic cancer.
abstract: The tumorigenesis of pancreatic cancer is thought to be a complex process. Investigation of the molecular mechanism of pancreatic cancer and exploring the specific markers for early diagnosis and specific targets of therapy is a key point to prevent and treat pancreatic cancer effectively and to improve their prognosis. In this study, expression profiles experiment was performed using Agilent human whole genomic oligonucleotide microarrays with 41,000 genes. Differentially expressed genes related with pancreatic cancer were screened, and analyzed further by GO term analysis and KEGG Pathway analysis. Our results showed that there were 1276 differentially expressed genes associated with pancreatic cancer. 691 genes were up regulated and 585 were down regulated in pancreatic cancer group. The present study confirmed that the occurrence of pancreatic cancer was involved in multiple-gene interaction. In addition, our study found that pancreatic cancer was related to an activation of the mTOR signaling pathway and renal cell carcinoma pathway.
authors.affiliation: Department of Gastroenterology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: He
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Shasha
authors.initials: S
authors.lastname: Geng
authors.forename: Haihui
authors.initials: H
authors.lastname: Sheng
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Shen
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Shizhang
authors.initials: S
authors.lastname: Zhu
authors.forename: Ximei
authors.initials: X
authors.lastname: Chen
authors.forename: Changqing
authors.initials: C
authors.lastname: Yang
authors.forename: Hengjun
authors.initials: H
authors.lastname: Gao
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D030541:Databases, Genetic; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055106:Genome-Wide Association Study; D006801:Humans; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D009363:Neoplasm Proteins / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D011230:Precancerous Conditions / Q000235:genetics* / Q000473:pathology; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000235:genetics; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases