title: Analysis of Pacific oyster larval proteome and its response to high-CO2.
abstract: Most calcifying organisms show depressed metabolic, growth and calcification rates as symptoms to high-CO(2) due to ocean acidification (OA) process. Analysis of the global expression pattern of proteins (proteome analysis) represents a powerful tool to examine these physiological symptoms at molecular level, but its applications are inadequate. To address this knowledge gap, 2-DE coupled with mass spectrophotometer was used to compare the global protein expression pattern of oyster larvae exposed to ambient and to high-CO(2). Exposure to OA resulted in marked reduction of global protein expression with a decrease or loss of 71 proteins (18% of the expressed proteins in control), indicating a wide-spread depression of metabolic genes expression in larvae reared under OA. This is, to our knowledge, the first proteome analysis that provides insights into the link between physiological suppression and protein down-regulation under OA in oyster larvae.
authors.affiliation: The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Hong Kong.
authors.forename: R
authors.initials: R
authors.lastname: Dineshram
authors.forename: Kelvin K W
authors.initials: KK
authors.lastname: Wong
authors.forename: Shu
authors.initials: S
authors.lastname: Xiao
authors.forename: Ziniu
authors.initials: Z
authors.lastname: Yu
authors.forename: Pei Yuan
authors.initials: PY
authors.lastname: Qian
authors.forename: Vengatesen
authors.initials: V
authors.lastname: Thiyagarajan
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000633:toxicity*; D004784:Environmental Monitoring; D015870:Gene Expression / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000187:drug effects* / Q000502:physiology; D010125:Ostreidae / Q000502:physiology*; D020543:Proteome / Q000378:metabolism*; D012623:Seawater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D020543:Proteome; D014874:Water Pollutants, Chemical; D002245:Carbon Dioxide