title: Photosynthetic and physiological analysis of the rice high-chlorophyll mutant (Gc).
abstract: Chlorophyll (Chl) molecules are essential for harvesting light energy in photosynthesis. A rice high-chlorophyll mutant (Gc) with significantly increased Chl b was identified previously in Zhenshan 97B (Oryza sativa indica). However, the mechanism underlying this higher Chl b content and its effects on photosynthetic efficiency are still unclear. Immunoblot and blue native polyacrylamide gel electrophoresis (BN-PAGE) with a second dimension electrophoresis followed by the matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) analysis showed that a few core proteins of photosystem I (PSI) and photosystem II (PSII), and light-harvesting complex II (LHCII) proteins were overexpressed in the mutant plants. Remarkable differences in chloroplast ultrastructure were observed between the wild-type and mutant plants, with the latter having more highly stacked and larger grana. Chl florescence analysis demonstrated that Gc had markedly increased quantum efficiency of photosystem II (ΦPSII), photochemical quenching (qP), non-photochemical quenching (qN) and electron transport rate (ETR). This morphological and physiological adaptation might confer a higher photosynthetic capacity in Gc than the wild-type.
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, China.
authors.forename: Zhenhui
authors.initials: Z
authors.lastname: Kang
authors.forename: Guanrong
authors.initials: G
authors.lastname: Li
authors.forename: Junli
authors.initials: J
authors.lastname: Huang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Niu
authors.forename: Hanyan
authors.initials: H
authors.lastname: Zou
authors.forename: Guangchao
authors.initials: G
authors.lastname: Zang
authors.forename: Yihao
authors.initials: Y
authors.lastname: Wenwen
authors.forename: Guixue
authors.initials: G
authors.lastname: Wang
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism* / Q000648:ultrastructure; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D009154:Mutation; D063347:Native Polyacrylamide Gel Electrophoresis; D012275:Oryza / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D010788:Photosynthesis / Q000502:physiology*; D045331:Photosystem I Protein Complex / Q000378:metabolism*; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D018515:Plant Leaves / Q000502:physiology; D018749:RNA, Plant / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D060431:Thylakoid Membrane Proteins / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D045342:Light-Harvesting Protein Complexes; D045331:Photosystem I Protein Complex; D045332:Photosystem II Protein Complex; D018749:RNA, Plant; D060431:Thylakoid Membrane Proteins; D002734:Chlorophyll