title: The protective effects of omega 3 fatty acids and sesame oil against cyclosporine A-induced nephrotoxicity.
abstract: Cyclosporine A (CsA) is a drug used in autoimmune disorders and organ transplantations. Omega 3 fatty acids (O-3) and sesame oil (SO) have antioxidant properties. We aimed to investigate and compare the protective effects of O-3 and SO against CsA-induced nephrotoxicity. Seven groups of male Wistar albino rats were included in the study. In group 1 (control), saline was administered, and in group 2, CsA (subcutaneously) was administered. In group 3, CsA+SO (orally; p.o.) was given; in group 4, CsA+O-3 (p.o.) was given; and in group 5, CsA+SO+O-3 was given. In group 6, SO was administered, and in group 7, O-3 was administered. After 15 days of treatment, kidneys were excised. Histopathological evaluation, apoptotic cell count, and renal/hepatic function tests were performed. In group 2, vacuolar degeneration and necrosis of tubular cells as well as hemorrhagic foci were observed; the apoptotic cell number was higher than in the control (P < 0.001). In groups 3, 4, and 5, tubular scores and apoptotic cell count were lower than in group 2 (P < 0.01 and P < 0.001, respectively). In groups 6 and 7, healthy renal histology and a few apoptotic cells were determined. In groups 2, 3, 4, and 5, blood urea nitrogen was higher and albumin was lower than in the control (P < 0.001). Liver enzymes were unchanged. O-3/SO showed similar protective effects against CsA-induced nephrotoxicity, as revealed by a remarkable decrease in histopathological changes and apoptotic cell count. However, impaired renal function tests were not improved with O-3/SO treatment. SO and O-3 can be used as chemoprotectants against CsA.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey. yasemin.goksu@gmail.com
authors.forename: Azize Yasemin
authors.initials: AY
authors.lastname: Goksu Erol
authors.forename: Gulcan
authors.initials: G
authors.lastname: Avcı
authors.forename: Alper
authors.initials: A
authors.lastname: Sevimli
authors.forename: Elmas
authors.initials: E
authors.lastname: Ulutas
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozdemir
mesh_terms: D000284:Administration, Oral; D000418:Albumins / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001806:Blood Urea Nitrogen; D016572:Cyclosporine / Q000633:toxicity*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D007166:Immunosuppressive Agents / Q000633:toxicity; D007279:Injections, Subcutaneous; D007674:Kidney Diseases / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control*; D007677:Kidney Function Tests; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012715:Sesame Oil / Q000494:pharmacology*
chemical_list: D000418:Albumins; D000975:Antioxidants; D015525:Fatty Acids, Omega-3; D007166:Immunosuppressive Agents; D012715:Sesame Oil; D016572:Cyclosporine