title: Occurrence of ε-proteobacterial species in rabbits (Oryctolagus cuniculus) reared in intensive and rural farms.
abstract: In order to investigate the occurrence of Campylobacter, Helicobacter and Arcobacter species in caecal contents of rabbits reared in intensive and rural farms, a total of 87 samples from animals belonging to 29 farms were analysed by both cultural and PCR analyses. PCR analysis directly from faecal samples detected 100% positive samples for Campylobacter genus, 3.4% for Helicobacter genus and none for Arcobacter genus. 83 out of 87 animals (95.4%) and all the 29 farms were positive for Campylobacter cuniculorum as also determined by cultural examination. Campylobacter coli and Campylobacter jejuni were isolated only from three animals reared in two rural farms. No Helicobacter and Arcobacter species were isolated. To evaluate a possible genetic variability, one strain of C. cuniculorum from each farm was analysed by Pulsed Field Gel Electrophoresis (PFGE) and Amplified Fragment Length Polymorphism (AFLP). Genotyping revealed that C. cuniculorum population is heterogeneous among the different sources and no dominant clone has spread in the investigated farms. This survey highlighted a high presence of C. cuniculorum with a high rate of intestinal colonization, low presence of C. jejuni-coli, Helicobacter spp. and any Arcobacter spp. in farmed rabbits.
authors.affiliation: Department of Veterinary Medical Sciences, Alma Mater Studiorum, University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy. joana.revez@helsinki.fi
authors.forename: J
authors.initials: J
authors.lastname: Revez
authors.forename: M
authors.initials: M
authors.lastname: Rossi
authors.forename: S
authors.initials: S
authors.lastname: Piva
authors.forename: D
authors.initials: D
authors.lastname: Florio
authors.forename: A
authors.initials: A
authors.lastname: Lucchi
authors.forename: A
authors.initials: A
authors.lastname: Parisi
authors.forename: G
authors.initials: G
authors.lastname: Manfreda
authors.forename: R G
authors.initials: RG
authors.lastname: Zanoni
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D000829:Animals, Domestic; D002432:Cecum / Q000382:microbiology; D016521:Electrophoresis, Gel, Pulsed-Field; D020565:Epsilonproteobacteria / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016998:Helicobacter / Q000235:genetics / Q000302:isolation & purification*; D007558:Italy / Q000453:epidemiology; D011817:Rabbits / Q000382:microbiology*