title: Two-stage in situ gas stripping for enhanced butanol fermentation and energy-saving product recovery.
abstract: Two-stage gas stripping for butanol recovery from acetone-butanol-ethanol (ABE) fermentation with Clostridium acetobutylicum JB200 in a fibrous bed bioreactor was studied. Compared to fermentation without in situ gas stripping, more ABE (10.0 g/L acetone, 19.2 g/L butanol, 1.7 g/L ethanol vs. 7.9 g/L acetone, 16.2 g/L butanol, 1.4 g/L ethanol) were produced, with a higher butanol yield (0.25 g/g vs. 0.20 g/g) and productivity (0.40 g/L·h vs. 0.30 g/L·h) due to reduced butanol inhibition. The first-stage gas stripping produced a condensate containing 175.6 g/L butanol (227.0 g/L ABE), which after phase separation formed an organic phase containing 612.3g/L butanol (660.7 g/L ABE) and an aqueous phase containing 101.3 g/L butanol (153.2 g/L ABE). After second-stage gas stripping, a highly concentrated product containing 420.3 g/L butanol (532.3 g/L ABE) was obtained. The process is thus effective in producing high-titer butanol that can be purified with much less energy.
authors.affiliation: Department of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Xue
authors.forename: Jingbo
authors.initials: J
authors.lastname: Zhao
authors.forename: Fangfang
authors.initials: F
authors.lastname: Liu
authors.forename: Congcong
authors.initials: C
authors.lastname: Lu
authors.forename: Shang-Tian
authors.initials: ST
authors.lastname: Yang
authors.forename: Feng-Wu
authors.initials: FW
authors.lastname: Bai
mesh_terms: D000096:Acetone / Q000378:metabolism; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D003246:Conservation of Energy Resources; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005740:Gases / Q000737:chemistry*; D007700:Kinetics; D013997:Time Factors
chemical_list: D000440:Butanols; D005740:Gases; D000096:Acetone; D000431:Ethanol