title: Analytical procedure for the determination of Ethyl Lauroyl Arginate (LAE) to assess the kinetics and specific migration from a new antimicrobial active food packaging.
abstract: Ethyl Lauroyl Arginate (LAE) is a cationic tensoactive compound, soluble in water, with a wide activity spectrum against moulds and bacteria. LAE has been incorporated as antimicrobial agent into packaging materials for food contact and these materials require to comply with the specific migration criteria. In this paper, one analytical procedure has been developed and optimized for the analysis of LAE in food simulants after the migrations tests. It consists of the formation of an ionic pair between LAE and the inorganic complex Co(SCN)(4)(2-) in aqueous solution, followed by a liquid-liquid extraction in a suitable organic solvent and further UV-Vis absorbance measurement. In order to evaluate possible interferences, the ionic pair has been also analyzed by high performance liquid chromatography with UV-Vis detection. Both procedures provided similar analytical characteristics, with linear ranges from 1.10 to 25.00 mg kg(-1), linearity higher than 0.9886, limits of detection and quantification of 0.33 and 1.10 mg kg(-1), respectively, accuracy better than 1% as relative error and precision better than 3.6% expressed as RSD. Optimization of analytical techniques, thermal and chemical stability of LAE, as well as migration kinetics of LAE from experimental active packaging are reported and discussed.
authors.affiliation: Department of Analytical Chemistry, Aragon Institute of Engineering Research, EINA - University of Zaragoza, Spain.
authors.forename: Davinson
authors.initials: D
authors.lastname: Pezo
authors.forename: Beatriz
authors.initials: B
authors.lastname: Navascués
authors.forename: Jesús
authors.initials: J
authors.lastname: Salafranca
authors.forename: Cristina
authors.initials: C
authors.lastname: Nerín
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D001120:Arginine / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003035:Cobalt / Q000032:analysis / Q000737:chemistry*; D018857:Food Packaging; D007700:Kinetics; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods*; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013861:Thiocyanates / Q000032:analysis / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D012996:Solutions; D013861:Thiocyanates; D014867:Water; C082955:cobalt(II) tetrathiocyanate; D003035:Cobalt; C031371:N(alpha)-lauroylarginine ethyl ester; D001120:Arginine