title: Changes in membrane lipids and carotenoids during light acclimation in a marine cyanobacterium Synechococcus sp.
abstract: Time course of carotenoid and membrane lipid variation during high light (HL) acclimation (about 85 meu mol m-2 s-1), after transfer from low light (LL) (5-10 meu mol m-2 s-1), was determined in a marine Synechococcus strain. Highperformance liquid chromatography (HPLC) coupled to diode array detector (DAD) or electrospray ionization mass spectrometry (ESI-MS) was used for compound  separation and detection. Myxoxanthophyll rose within a time interval of 8 h to 24 h after the onset of exposure to HL.  Beta -carotene content started to decrease after 4 h of the onset of exposure to HL. Zeaxanthin content rose with exposure to HL,  but it was only significant after 24 h of exposure. Carotenoid changes are in agreement with a coordinated activity of the  enzymes of the myxoxanthophyll biosynthetic pathway, with no rate-limiting intermediate steps. Lipid analysis showed all species  with a C18:3/C16:0 composition increased their content, the changes of PG (18:3/16:0) and MGDG(18:3/16:0) being primarily  significant. Major lipid changes were also found to occur within 24 h. These changes might suggest reduction and  reorganization of the thylakoid membrane structure. Hypotheses are also drawn on the role played by lipid molecule shape and  their possible effect in membrane fluidity and protein accommodation.
authors.affiliation: Centre for Biotechnology Development, CSIC, Francisco Valles 8, 47151 Boecillo (Technological Park), Valladolid, Spain. olimpio.montero@dicyl.csic.es
authors.forename: Olimpio
authors.initials: O
authors.lastname: Montero
authors.forename: Alberto
authors.initials: A
authors.lastname: Sánchez-Guijo
authors.forename: Luis M
authors.initials: LM
authors.lastname: Lubián
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Martínez-Rodríguez
mesh_terms: D002338:Carotenoids / Q000378:metabolism*; D008027:Light; D008563:Membrane Lipids / Q000378:metabolism*; D046940:Synechococcus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D024341:Xanthophylls / Q000378:metabolism; D065146:Zeaxanthins
chemical_list: D008563:Membrane Lipids; D024341:Xanthophylls; D065146:Zeaxanthins; D002338:Carotenoids