title: Marginal zone B cell is a major source of Il-10 in Listeria monocytogenes susceptibility.
abstract: Rag-1-knockout (KO) mice are highly resistant to Listeria monocytogenes infection. The role played by the many Rag-1-dependent lymphocyte lineages was studied using a genetic approach in which each Rag-1-dependent lymphocyte lineage was eliminated one at a time. Only B cell-deficient Igh-KO mice displayed reduced bacterial load and improved survival upon Listeria infection. Listeria infection of Rag-1-KO and Il-10-KO hosts that had been adoptively transferred with wild-type marginal zone B (MZB) cells, but not follicular B cells, resulted in heightened bacterial load and increased Il-10 production in the spleen, but not the liver. This MZB cell-dependent increase in bacterial load was eliminated by anti-Il-10 mAb. In addition, Listeria infection of MZB cell-deficient Rbpj-cKO mice showed decreased bacterial load and increased survival. Whereas multiple cell types have been shown to be capable of Il-10 production, our results indicate that the MZB cell is the most dominant and relevant Il-10 source in the context of Listeria susceptibility. In marked contrast to the generally protective nature of MZB cells in defending against pathogenic infection, our results demonstrate that MZB cells play a detrimental role in Listeria infection and possibly other infections as well.
authors.affiliation: Graduate Institute of Immunology, College of Medicine, National Taiwan University, Taipei 10051, Taiwan, Republic of China.
authors.forename: Chen-Cheng
authors.initials: CC
authors.lastname: Lee
authors.forename: John T
authors.initials: JT
authors.lastname: Kung
mesh_terms: D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000382:microbiology* / Q000637:transplantation; D058491:Bacterial Load / Q000235:genetics / Q000276:immunology; D002470:Cell Survival / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis / Q000378:metabolism* / Q000502:physiology; D008088:Listeriosis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D013154:Spleen / Q000276:immunology* / Q000382:microbiology* / Q000637:transplantation; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
chemical_list: C556062:IL10 protein, mouse; D016753:Interleukin-10