title: Coupling diffusion imaging with histological and gene expression analysis to examine the dynamics of cortical areas across the fetal period of human brain development.
abstract: As a prominent component of the human fetal brain, the structure of the cerebral wall is characterized by its laminar organization which includes the radial glial scaffold during fetal development. Diffusion tensor imaging (DTI) is useful to quantitatively delineate the microstructure of the developing brain and to clearly identify transient fetal layers in the cerebral wall. In our study, the spatio-temporal microstructural changes in the developing human fetal cerebral wall were quantitatively characterized with high-resolution DTI data of postmortem fetal brains from 13 to 21 gestational weeks. Eleven regions of interest for each layer in the entire cerebral wall were included. Distinctive time courses of microstructural changes were revealed for 11 regions of the neocortical plate. A histological analysis was also integrated to elucidate the relationship between DTI fractional anisotropy (FA) and histology. High FA values correlated with organized radial architecture in histological image. Expression levels of 17565 genes were quantified for each of 11 regions of human fetal neocortex from 13 to 21 gestational weeks to identify transcripts showing significant correlation with FA change. These correlations suggest that the heterogeneous and regionally specific microstructural changes of the human neocortex are related to different gene expression patterns.
authors.affiliation: Advanced Imaging Research Center.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.forename: Tina
authors.initials: T
authors.lastname: Jeon
authors.forename: Goran
authors.initials: G
authors.lastname: Sedmak
authors.forename: Mihovil
authors.initials: M
authors.lastname: Pletikos
authors.forename: Lana
authors.initials: L
authors.lastname: Vasung
authors.forename: Xuming
authors.initials: X
authors.lastname: Xu
authors.forename: Paul
authors.initials: P
authors.lastname: Yarowsky
authors.forename: Linda J
authors.initials: LJ
authors.lastname: Richards
authors.forename: Ivica
authors.initials: I
authors.lastname: Kostovic
authors.forename: Nenad
authors.initials: N
authors.lastname: Sestan
authors.forename: Susumu
authors.initials: S
authors.lastname: Mori
mesh_terms: D002540:Cerebral Cortex / Q000033:anatomy & histology* / Q000196:embryology* / Q000378:metabolism; D056324:Diffusion Tensor Imaging; D005333:Fetus / Q000033:anatomy & histology* / Q000378:metabolism; D020869:Gene Expression Profiling; D005865:Gestational Age; D006801:Humans