title: Family-based linkage and association mapping reveals novel genes affecting Plum pox virus infection in Arabidopsis thaliana.
abstract: Sharka is a devastating viral disease caused by the Plum pox virus (PPV) in stone fruit trees and few sources of resistance are known in its natural hosts. Since any knowledge gained from Arabidopsis on plant virus susceptibility factors is likely to be transferable to crop species, Arabidopsis's natural variation was searched for host factors essential for PPV infection. To locate regions of the genome associated with susceptibility to PPV, linkage analysis was performed on six biparental populations as well as on multiparental lines. To refine quantitative trait locus (QTL) mapping, a genome-wide association analysis was carried out using 147 Arabidopsis accessions. Evidence was found for linkage on chromosomes 1, 3 and 5 with restriction of PPV long-distance movement. The most relevant signals occurred within a region at the bottom of chromosome 3, which comprises seven RTM3-like TRAF domain-containing genes. Since the resistance mechanism analyzed here is recessive and the rtm3 knockout mutant is susceptible to PPV infection, it suggests that other gene(s) present in the small identified region encompassing RTM3 are necessary for PPV long-distance movement. In consequence, we report here the occurrence of host factor(s) that are indispensable for virus long-distance movement.
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Pagny
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Pauline S
authors.initials: PS
authors.lastname: Paulstephenraj
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Poque
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Ophélie
authors.initials: O
authors.lastname: Sicard
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Cosson
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Eyquard
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Mélodie
authors.initials: M
authors.lastname: Caballero
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Chague
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Germain
authors.initials: G
authors.lastname: Gourdon
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Lise
authors.initials: L
authors.lastname: Negrel
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Candresse
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Mariette
authors.affiliation: INRA, Université de Bordeaux, UMR 1332 BFP, BP81, 33883, Villenave d'Ornon Cedex, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Decroocq
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000378:metabolism; D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics / Q000378:metabolism; D003433:Crosses, Genetic; D060467:Disease Resistance; D055786:Gene Knockout Techniques; D017343:Genes, Plant; D056726:Genetic Association Studies / Q000379:methods; D008040:Genetic Linkage; D018745:Genome, Plant; D018895:Microsatellite Repeats; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000821:virology; D017801:Plum Pox Virus / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*; D054817:Pollination; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci
chemical_list: D029681:Arabidopsis Proteins; C554275:RTM3 protein, Arabidopsis