title: [Determination of virginiamycin M1 and S1 residues in livestock and poultry products by liquid chromatography-tandem mass spectrometry].
abstract: A liquid chromatography-tandem mass spectrometry method was established for the determination of virginiamycin M1 and S1 residues in livestock and poultry products. The sample was extracted by methanol-acetonitrile solution (1:1, v/v). The supernatant was diluted with 0.01 mol/L ammonium dihydrogen phosphate solution, then purified and concentrated on an Oasis HLB cartridge. The separation of virginiamycin M1 and S1 was performed on a Luna C18 column with the mobile phases acetonitrile and 5 mmol/L ammonium acetate aqueous solution (containing 0.1% (v/v) formic acid) in a gradient elution mode. The identification and quantification of the drugs were carried out by positive electrospray ionization (ESI + ) in the multiple reaction monitoring (MRM) mode using external standard method. The calibration curves showed good linearity in the range of 0.15-10.0 microg/L with correlation coefficients (r2) above 0. 999. The limits of quantities (LOQs) were both 0.25 microg/kg. The average recoveries of the two drugs spiked at 0.25, 0.5 and 2.5 microg/kg levels in different matrices were between 71.2% and 98.4%, and the relative standard deviations (RSDs) were between 3.6% and 15.4%. The method is simple, rapid, sensitive and accurate. It is suitable for the confirmation and quantification of virginiamycin M1 and S1 residues in livestock and poultry products.
authors.affiliation: Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China. yjqiuzju@163.com
authors.forename: Yuanjin
authors.initials: Y
authors.lastname: Qiu
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Zhengcai
authors.initials: Z
authors.lastname: Liu
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Lin
authors.forename: Suzhen
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination / Q000032:analysis; D058751:Livestock; D008461:Meat Products / Q000032:analysis*; D011200:Poultry; D025364:Streptogramin A / Q000032:analysis*; D025382:Streptogramin Group B / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014769:Virginiamycin / Q000032:analysis*
chemical_list: D025382:Streptogramin Group B; D014769:Virginiamycin; D025364:Streptogramin A; C418083:virginiamycin factor S1