title: Evaluation of the genotoxicity of extracts of Houttuynia cordata Thunb.
abstract: The present study was conducted to evaluate the activity of methanol extracts from Houttuynia cordata Thunb. (HC) in a reverse mutation assay in Salmonella typhimurium, and a chromosome aberration assay in the Chinese hamster ovary (CHO) cell line and to evaluate its effect on the occurrence of polychromatic erythrocytes in mice. In the reverse mutation assay using Salmonella typhimurium TA98, TA100, TA1535, and TA1537 and Escherichia coli WP2urvA(-), methanol extracts of HC (5, 2.5, 1.25, 0.62, or 0.312 mg/plate) did not induce reverse mutations in the presence or absence of an S9 metabolic activation mixture. In the chromosome aberration test using CHO cells, methanol extracts (1.25, 2.5 or 5 μg/ml) caused a few incidences of structural and numerical aberrations, in both of absence or presence of an S9 metabolic activation mixture, but in comparison with the positive control group, these incidences were not significantly increased. In the mouse micronucleus test, no significant increases in the occurrence of micronucleated polychromatic erythrocytes were observed in male ICR mice that were orally administered methanol extracts of HC at doses of 2.0, 1.0, or 0.5 g/kg. From these results, we concluded that the methanol extracts of HC did not induce harmful effects on genes in bacteria, a mammalian cell system or in mouse bone marrow cells. Thus, HC's use for health promotion and/or a sick remedy for humans may be safe.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine and Institute Animal Science, Gyeongsang National University, Gyeongnam 660-701,  Korea.
authors.forename: Chang Keun
authors.initials: CK
authors.lastname: Kang
authors.forename: Dae Sik
authors.initials: DS
authors.lastname: Hah
authors.forename: Chung Hui
authors.initials: CH
authors.lastname: Kim
authors.forename: Euikyung
authors.initials: E
authors.lastname: Kim
authors.forename: Jong Shu
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D052859:Houttuynia / Q000009:adverse effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D048629:Micronuclei, Chromosome-Defective; D009154:Mutation; D010936:Plant Extracts / Q000009:adverse effects*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
chemical_list: D010936:Plant Extracts