title: A multiagent filovirus DNA vaccine delivered by intramuscular electroporation completely protects mice from ebola and Marburg virus challenge.
abstract: We evaluated the immunogenicity and protective efficacy of DNA vaccines expressing the codon-optimized envelope glycoprotein genes of Zaire ebolavirus, Sudan ebolavirus, and Marburg marburgvirus (Musoke and Ravn). Intramuscular or intradermal delivery of the vaccines in BALB/c mice was performed using the TriGrid™ electroporation device. Mice that received DNA vaccines against the individual viruses developed robust glycoprotein-specific antibody titers as determined by ELISA and survived lethal viral challenge with no display of clinical signs of infection. Survival curve analysis revealed there was a statistically significant increase in survival compared to the control groups for both the Ebola and Ravn virus challenges. These data suggest that further analysis of the immune responses generated in the mice and additional protection studies in nonhuman primates are warranted.
authors.affiliation: U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA. Rebecca.J.Klein@us.army.mil
authors.forename: Rebecca J
authors.initials: RJ
authors.lastname: Grant-Klein
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Van Deusen
authors.forename: Catherine V
authors.initials: CV
authors.lastname: Badger
authors.forename: Drew
authors.initials: D
authors.lastname: Hannaman
authors.forename: Lesley C
authors.initials: LC
authors.lastname: Dupuy
authors.forename: Connie S
authors.initials: CS
authors.lastname: Schmaljohn
mesh_terms: D000818:Animals; D029043:Ebolavirus / Q000276:immunology / Q000472:pathogenicity; D018274:Electroporation / Q000379:methods*; D005260:Female; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology / Q000517:prevention & control*; D008379:Marburg Virus Disease / Q000276:immunology / Q000517:prevention & control*; D029024:Marburgvirus / Q000276:immunology / Q000472:pathogenicity; D051379:Mice; D008807:Mice, Inbred BALB C; D009132:Muscles / Q000378:metabolism*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000627:therapeutic use*
chemical_list: D019444:Vaccines, DNA