title: Development of 35 microsatellite markers from heat stress transcription factors in Populus simonii (Salicaceae).
abstract: OBJECTIVE
  Microsatellite markers within regulators of heat stress transcription factors were identified in the Populus trichocarpa genome, and then developed for P. simonii to investigate the genetic diversity of germplasm resources and to further identify favorable alleles significantly associated with stress-resistant traits.
  
  
  RESULTS
  Thirty-five novel microsatellite markers were identified from genes controlling heat stress transcription factors in P. simonii using a Sanger sequencing protocol. Polymorphisms in 48 individuals from 16 populations of P. simonii revealed that the number of alleles per locus ranged from two to nine with an average of 4.6; the observed heterozygosity and expected heterozygosity per locus varied from 0.143 to 0.857 and from 0.257 to 0.948, respectively.
  
  
  CONCLUSIONS
  The new polymorphic markers developed during this study will facilitate the construction of genetic linkage maps and will aid in marker-assisted breeding of a new germplasm with desirable abiotic stress resistance in Populus species.
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Bao-Lei
authors.initials: BL
authors.lastname: Wang
authors.forename: Zun-Zheng
authors.initials: ZZ
authors.lastname: Wei
authors.forename: Qing-Zhang
authors.initials: QZ
authors.lastname: Du
authors.forename: De-Qiang
authors.initials: DQ
authors.lastname: Zhang
authors.forename: Bai-Lian
authors.initials: BL
authors.lastname: Li
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D014644:Genetic Variation; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D032107:Populus / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D014157:Transcription Factors