title: Expression of efflux pump gene lde in ciprofloxacin-resistant foodborne isolates of Listeria monocytogenes.
abstract: The expression of efflux pump gene lde in ciprofloxacin resistant (Cip(R) ) and susceptible strains of Listeria monocytogenes collected from retail food samples was investigated. For two Cip(R)  strains, the MICs of ciprofloxacin decreased four- to eightfold in the presence of reserpine; however, no significant alterations were observed with naturally sensitive isolates. Overexpression of the lde gene induced by ciprofloxacin was observed in two resistant isolates. The present findings indicate that expression of lde and the MICs of ciprofloxacin are well correlated with the presence and absence of reserpine, suggesting that Lde might be involved in ciprofloxacin resistance of L. monocytogenes.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Jiang
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhou
authors.forename: Dongwei
authors.initials: D
authors.lastname: Gao
authors.forename: Yuxiao
authors.initials: Y
authors.lastname: Wang
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Miaorui
authors.initials: M
authors.lastname: Chen
authors.forename: Yongyu
authors.initials: Y
authors.lastname: Su
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: He
authors.initials: H
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D005516:Food Microbiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests
chemical_list: D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin