title: Knockdown of RAB25 promotes autophagy and inhibits cell growth in ovarian             cancer cells.
abstract: RAB25 belongs to the Rab family of small GTPases and is implicated in the             development of various types of human cancer. To evaluate the role of RAB25 in             ovarian cancer, RAB25 was knocked down by siRNA in HEY and ES‑2 human ovarian             cancer cells. Autophagy, cell growth and cell apoptosis were evaluated. The results             showed that knockdown of RAB25 increased acidic vesicle organelles and GFP-microtubule-associated             protein 1 light chain 3 punctate fluorescence in ovarian cancer cells. Autophagy             that promoted by knockdown of RAB25 was not observed in cells where the ERK1/2             signaling pathway had been inhibited by U0126. Knockdown of RAB25 reduced cell             cycle progression and cell growth. Apoptosis of ovarian cancer cells could be             induced by knockdown of RAB25. These results support the tumorigenic role of RAB25             in ovarian cancer cells.
authors.affiliation: Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, PR China.
authors.forename: Yingtao
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Tao
authors.forename: Luoqi
authors.initials: L
authors.lastname: Jia
authors.forename: Kwai Wa
authors.initials: KW
authors.lastname: Cheng
authors.forename: Yiling
authors.initials: Y
authors.lastname: Lu
authors.forename: Yinhua
authors.initials: Y
authors.lastname: Yu
authors.forename: Youji
authors.initials: Y
authors.lastname: Feng
mesh_terms: D017209:Apoptosis; D001343:Autophagy; D002070:Butadienes / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D020691:rab GTP-Binding Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism
chemical_list: D002070:Butadienes; D009570:Nitriles; D034741:RNA, Small Interfering; C509763:Rab25 protein, human; C113580:U 0126; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020691:rab GTP-Binding Proteins