title: A study of peripheral blood in hedgehogs in Turkey.
abstract: The aim of this study was to determine diameters of blood cells, differential counts of peripheral blood leukocytes, alpha-naphthyl acetate esterase (ANAE), acid phosphatase (ACP-ase) activity of some leukocyte types, and enzymatic positivity percentages of peripheral blood lymphocytes in two hedgehogs species, Hemiechinus auritus, the long-eared hedgehog, and Erinaceus concolor, the southern white-breasted hedgehog. Air-dried peripheral blood smears were stained with May-Grünwald-Giemsa stain. ANAE and ACP-ase were stained in glutaraldehyde-acetone-fixed smears. ANAE-positive lymphocytes displayed a dot-like positivity pattern characterized with 1-5 reddish brown cytoplasmic granules, whereas ACP-ase positive lymphocytes displayed a dot-like positivity pattern characterized with 1-3 pinkish cytoplasmic granules. Monocytes gave a diffuse and strong reaction while neutrophils displayed a weak positive reaction for ANAE and ACP-ase. No difference was observed in mean diameters of peripheral blood cells of these species. It was found that lymphocytes made up the majority (64.3% and 65.5%) of leukocytes, followed by neutrophils (23.9% and 23.3%), eosinophils (9.0% and 7.6%), monocytes (1.8% and 2.3%), and basophils (1.0% and 1.3%) in H. auritus and E. concolor, respectively. Mean ANAE positivity oflymphocytes was 36.6% and 51.3% and ACP-ase positivity was 32.1% and 37.5% for H. auritus and E. concolor, respectively. The ANAE positivity of lymphocytes in E. concolor was significantly (P < 0.05) higher than that of H. auritus.
authors.affiliation: Department of Biology, Faculty of Science, Selcuk University, Selçuklu, 42075, Konya, Turkey. hozparlak@selcuk.edu.tr
authors.forename: Haluk
authors.initials: H
authors.lastname: Ozparlak
authors.forename: Ilhami
authors.initials: I
authors.lastname: Celik
authors.forename: Emrah
authors.initials: E
authors.lastname: Sur
authors.forename: Tuğba
authors.initials: T
authors.lastname: Ozaydin
authors.forename: Atilla
authors.initials: A
authors.lastname: Arslan
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000166:cytology; D004912:Erythrocytes / Q000166:cytology; D006364:Hedgehogs / Q000097:blood*; D007958:Leukocyte Count / Q000662:veterinary*; D007962:Leukocytes / Q000166:cytology; D009283:Naphthol AS D Esterase / Q000097:blood; D014421:Turkey
chemical_list: D009283:Naphthol AS D Esterase