title: Validation of a bioanalytical method using capillary microsampling of 8 µl plasma samples: application to a toxicokinetic study in mice.
abstract: BACKGROUND
  Capillary microsampling was recently introduced as a new technique for simplified collection and handling of small, exact volumes of liquid matrices. In this article, a bioanalytical method was developed and fully validated for 8 µl plasma samples and applied to a toxicology study in mice.
  
  
  RESULTS
  The method was validated in the concentration range 0.06-30 µM. A procedure where 32 µl of blood was collected for preparation of 8 µl plasma was successfully implemented at the animal facility. All the results for the method and study validation met the requirements of a regulated assay.
  
  
  CONCLUSIONS
  It is shown that 8 µl plasma microsamples can be sampled and analyzed with consistently excellent accuracy and precision. Capillary microsampling of plasma offers a possibility to combine ethical, scientific and economic benefits while still maintaining the advantage of having drug exposure data in plasma.
authors.affiliation: Global DMPK, Screening and Profiling, AstraZeneca R&D, Södertälje, Sweden. ovex.jonsson@gmail.com
authors.forename: Ove
authors.initials: O
authors.lastname: Jonsson
authors.forename: Rodrigo Palma
authors.initials: RP
authors.lastname: Villar
authors.forename: Lars B
authors.initials: LB
authors.lastname: Nilsson
authors.forename: Marie
authors.initials: M
authors.lastname: Eriksson
authors.forename: Kristian
authors.initials: K
authors.lastname: Königsson
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D004364:Pharmaceutical Preparations / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D004364:Pharmaceutical Preparations