title: Role of HLA-G 14bp deletion/insertion and +3142C>G polymorphisms in the production of sHLA-G molecules in relapsing-remitting multiple sclerosis.
abstract: HLA-G is believed to act as an anti-inflammatory molecule in Multiple Sclerosis (MS). The 3' untranslated region of the HLA-G gene is characterized by two polymorphisms, DEL/INS14bp and +3142C>G, which control soluble HLA-G (sHLA-G) production. The influence of these two HLA-G variants on sHLA-G serum and cerebrospinal fluid (CSF) levels was investigated in 69 Relapsing-Remitting MS patients grouped in magnetic resonance imaging (MRI) inactive and active disease. Serum and CSF sHLA-G levels were more elevated in high than in low DEL/INS 14bp and +3142C>G sHLA-G producers and were different among the various combined HLA-G genotypes in both MRI inactive and active diseases. The highest and the lowest sHLA-G values were identified in MS patients with C/C,DEL/DEL and G/G,INS/INS genotypes, respectively. Our preliminary findings suggest that serum and CSF sHLA-G levels in MS could be influenced by HLA-G polymorphisms irrespective of the inflammatory microenvironment.
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Ferrara 44121, Italy. rbr@unife.it
authors.forename: Roberta
authors.initials: R
authors.lastname: Rizzo
authors.forename: Daria
authors.initials: D
authors.lastname: Bortolotti
authors.forename: Nadia Ben
authors.initials: NB
authors.lastname: Fredj
authors.forename: Antonella
authors.initials: A
authors.lastname: Rotola
authors.forename: Francesca
authors.initials: F
authors.lastname: Cura
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Castellazzi
authors.forename: Carmine
authors.initials: C
authors.lastname: Tamborino
authors.forename: Silva
authors.initials: S
authors.lastname: Seraceni
authors.forename: Eleonora
authors.initials: E
authors.lastname: Baldi
authors.forename: Loredana
authors.initials: L
authors.lastname: Melchiorri
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Tola
authors.forename: Enrico
authors.initials: E
authors.lastname: Granieri
authors.forename: Olavio R
authors.initials: OR
authors.lastname: Baricordi
authors.forename: Enrico
authors.initials: E
authors.lastname: Fainardi
mesh_terms: D000328:Adult; D000483:Alleles; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D059951:HLA-G Antigens / Q000097:blood / Q000134:cerebrospinal fluid / Q000235:genetics*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D020641:Polymorphism, Single Nucleotide; D017384:Sequence Deletion
chemical_list: D059951:HLA-G Antigens