title: Chemometric formulation of bacterial consortium-AVS for improved decolorization of resonance-stabilized and heteropolyaromatic dyes.
abstract: A bacterial consortium-AVS, consisting of Pseudomonas desmolyticum NCIM 2112, Kocuria rosea MTCC 1532 and Micrococcus glutamicus NCIM 2168 was formulated chemometrically, using the mixture design matrix based on the design of experiments methodology. The formulated consortium-AVS decolorized acid blue 15 and methylene blue with a higher average decolorization rate, which is more rapid than that of the pure cultures. The UV-vis spectrophotometric, Fourier transform infra red spectrophotometric and high performance liquid chromatographic analysis confirm that the decolorization was due to biodegradation by oxido-reductive enzymes, produced by the consortium-AVS. The toxicological assessment of plant growth parameters and the chlorophyll pigment concentrations of Phaseolus mungo and Triticum aestivum seedlings revealed the reduced toxic nature of the biodegraded products.
authors.affiliation: Department of Chemical Engineering, AC College of Technology, Anna University, Chennai, India.
authors.forename: Madhava Anil
authors.initials: MA
authors.lastname: Kumar
authors.forename: Vaidyanathan Vinoth
authors.initials: VV
authors.lastname: Kumar
authors.forename: Manickam Periyaraman
authors.initials: MP
authors.lastname: Premkumar
authors.forename: Palanichamy
authors.initials: P
authors.lastname: Baskaralingam
authors.forename: Kadathur Varathachary
authors.initials: KV
authors.lastname: Thiruvengadaravi
authors.forename: Anuradha
authors.initials: A
authors.lastname: Dhanasekaran
authors.forename: Subramanian
authors.initials: S
authors.lastname: Sivanesan
mesh_terms: D000704:Analysis of Variance; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003116:Color; D004396:Coloring Agents / Q000378:metabolism* / Q000633:toxicity; D007887:Fabaceae / Q000187:drug effects; D006841:Hydrocarbons, Aromatic / Q000378:metabolism*; D008751:Methylene Blue / Q000378:metabolism; D008903:Minerals / Q000737:chemistry; D008956:Models, Chemical; D010084:Oxidation-Reduction / Q000187:drug effects; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D018675:Toxicity Tests; D014908:Triticum / Q000187:drug effects
chemical_list: D004396:Coloring Agents; D006841:Hydrocarbons, Aromatic; D008903:Minerals; D008751:Methylene Blue