title: Ion-exchange protocol to obtain antigenic fractions with potential for serodiagnosis of strongyloidiasis.
abstract: The aim of this study was to fractionate and partially characterize the antigenic extract of filariform larvae of Strongyloides venezuelensis in ion-exchange resin diethylaminoethyl sepharose (DEAE), to obtain antigenic fractions potentially applicable in immunoassays. Somatic antigen (SA) and its fractions DEAE S1 and DEAE S2 - which interacted with the resin - were evaluated by 1-dimensional electrophoresis to obtain protein profiles. SA and its fractions were tested in serum samples for IgG detection by ELISA. Serum samples (n = 155) were analysed: 50 from strongyloidiasis patients (G1), 55 from patients with other parasitic infections (G2) and 50 from healthy volunteers. Sensitivity (Se), specificity (Sp), area under curve (AUC) and likelihood ratios (LR) were calculated. The DEAE S2 fraction provided a high diagnostic value for IgG detection (Se 92·0%, Sp 91·4%, AUC 0·981, LR+ 10·75, LR - 0·09). In conclusion, the DEAE S2 fraction would probably be a source of immunodominant polypeptides for IgG detection in human strongyloidiasis serodiagnosis.
authors.affiliation: Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil.
authors.forename: H T
authors.initials: HT
authors.lastname: Gonzaga
authors.forename: C
authors.initials: C
authors.lastname: Vila-Verde
authors.forename: D S
authors.initials: DS
authors.lastname: Nunes
authors.forename: V S
authors.initials: VS
authors.lastname: Ribeiro
authors.forename: J P
authors.initials: JP
authors.lastname: Cunha-Júnior
authors.forename: J M
authors.initials: JM
authors.lastname: Costa-Cruz
mesh_terms: D000818:Animals; D000947:Antigens, Helminth; D002852:Chromatography, Ion Exchange; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007814:Larva / Q000737:chemistry; D012680:Sensitivity and Specificity; D012698:Serologic Tests; D044967:Serum / Q000469:parasitology; D013321:Strongyloides / Q000737:chemistry*; D013322:Strongyloidiasis / Q000175:diagnosis*
chemical_list: D000947:Antigens, Helminth; D007074:Immunoglobulin G