title: Adsorption behavior of plasmid DNA onto perfusion chromatographic matrix.
abstract: Anion exchange chromatography is the most popular chromatographic method for plasmid separation. POROS RI 50 is a perfusion chromatographic support which is a reversed phase matrix and is an alternative to conventional ones due to its mass transfer properties. The adsorption and elution of the pIDKE2 plasmid onto reversed phase POROS R1 50 was studied. Langmuir isotherm model was adjusted in order to get the maximum adsorption capacity and the dissociation constant for POROS R1 50-plasmid DNA (pDNA) system. Breakthrough curves were obtained for volumetric flows between 0.69-3.33 mL/min, given dynamic capacity up to 2.3 times higher than those reported for ionic exchange matrix used during the purification process of plasmids with similar size to that of pIDKE2. The efficiency was less than 45% for the flow conditions and initial concentration studied, which means that the support will not be operated under saturation circumstances.
authors.affiliation: Center for Genetic Engineering and Biotechnology, Habana 10600, Cuba. miladys.limonta@cigb.edu.cu
authors.forename: Miladys
authors.initials: M
authors.lastname: Limonta
authors.forename: Lourdes
authors.initials: L
authors.lastname: Zumalacárregui
authors.forename: Dayana
authors.initials: D
authors.lastname: Soler
mesh_terms: D000327:Adsorption; D000818:Animals; D000837:Anion Exchange Resins / Q000737:chemistry; D000838:Anions / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D010957:Plasmids / Q000737:chemistry / Q000302:isolation & purification*; D016062:Porosity
chemical_list: D000837:Anion Exchange Resins; D000838:Anions; D004247:DNA