title: KP-CoT-23 (CCDC83) is a novel immunogenic cancer/testis antigen in colon             cancer.
abstract: Cancer/testis (CT) antigens are considered target molecules for cancer immunotherapy.             To identify novel CT antigens, immunoscreening of a testicular cDNA library was             performed using serum obtained from a colon cancer patient who was immunized with             a new dendritic cell vaccine. We isolated 64 positive cDNA clones comprised of             40 different genes, designated KP-CoT-1 through KP-CoT-40. Three of these putative             antigens, including KP-CoT-23 (CCDC83), had testis-specific expression profiles             in the Unigene database. RT-PCR analysis showed that the expression of 2 KP-Cot-23             variants was restricted to the testis in normal adult tissues. In addition, KP-CoT-23             variants were frequently expressed in a variety of tumors and cancer cell lines,             including colon cancer. A serological western blot assay showed IgG antibodies             to the KP-CoT-23 protein in 26 of 37 colon cancer patients and in 4 of 21 healthy             patients. These data suggest that KP-CoT-23 is a novel CT antigen that may be             useful for the diagnosis and immunotherapy of cancer.
authors.affiliation: Department of Biochemistry, School of Medicine, Pusan National                     University, Yangsan‑si, Gyeongsangnam‑do 626-770, Republic of Korea.
authors.forename: Myung-Ha
authors.initials: MH
authors.lastname: Song
authors.forename: Jin-Mok
authors.initials: JM
authors.lastname: Ha
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Shin
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: Lee
authors.forename: Lloyd
authors.initials: L
authors.lastname: Old
authors.forename: Sang-Yull
authors.initials: SY
authors.lastname: Lee
mesh_terms: D000906:Antibodies / Q000097:blood / Q000276:immunology; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000276:immunology* / Q000378:metabolism*
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; C549375:CCDC83 protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger