title: Detection of Chikungunya virus in wild populations of Aedes albopictus in Kerala State, India.
abstract: We detected Chikungunya virus (CHIKV) infection among wild populations of Aedes albopictus female specimens during the CHIKV outbreaks of 2009 and 2006 collected in different localities in Kerala State, India. The envelope 1 gene (E1) sequences of the virus isolate 2009 from the mosquito species showed close genetic relatedness (Kimura 2 Parameter genetic distance=0.0013) to CHIKV-positive isolates from human serum samples from the same area. E1 gene sequences from Ae. albopictus, as well as from human isolates, had the crucial non-synonymous C/T mutation at position 10670, leading to the A226V amino acid change. This natural inclination indicated the role of this mosquito species in the transmission of CHIKV during its recent outbreaks in Kerala State.
authors.affiliation: Vector Control Research Centre Field Station (ICMR), Kottayam, Kerala, India. kumar.dr.n.pradeep@gmail.com
authors.forename: Narendran Pradeep
authors.initials: NP
authors.lastname: Kumar
authors.forename: Shanmugavelu
authors.initials: S
authors.lastname: Sabesan
authors.forename: Kaliannagounder
authors.initials: K
authors.lastname: Krishnamoorthy
authors.forename: Purushothaman
authors.initials: P
authors.lastname: Jambulingam
mesh_terms: D000330:Aedes / Q000821:virology*; D018354:Alphavirus Infections / Q000453:epidemiology / Q000635:transmission* / Q000821:virology; D000818:Animals; D001483:Base Sequence; D065632:Chikungunya Fever; D002646:Chikungunya virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D007194:India / Q000453:epidemiology; D007303:Insect Vectors / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014759:Viral Envelope Proteins