title: [Detection of mercury (II) ions (Hg(2+)) in water by a nanobiosensor].
abstract: OBJECTIVE
  To develop a nanobiosensor for rapid colorimetric detecting Mercury (II) Ions (Hg(2+)) in water by mercury-specific oligonucleotides (MSOs) probe and gold nanoparticles.
  
  
  METHODS
  The nanobiosensor was assembled by adsorbing the optimized MSOs on the surface of gold nanoparticles. A direct colorimetric probe of Hg(2+) which relied on the T-T mismatches in DNA duplexes was used to selectively and strongly capture Hg(2+). Hg(2+) induces the aggregation of gold nanoparticles with appropriate amount of salts, resulting the color change (red to blue).
  
  
  RESULTS
  The diameter and concentration of the gold nanoparticle preparation were 15 nm and 2.97 nmol/L, respectively. Truncated MSOs (9 bp) showed the similar Hg(2+)-binding activity. The optimum concentration of the NaNO3 solution was 0.5 mol/L. The nanobiosensor could detect Hg(2+)in a range of 10 ∼ 1000 µmol/L within few minutes and the specificity was 100%.
  
  
  CONCLUSIONS
  A new nanobiosensor is developed successfully for rapid colorimetric detecting Hg(2+) in water, avoiding either MSOs labeling or gold nanoparticles modification. This technique is simple, convenient and rapid detecting method with high sensitivity and specificity.
authors.affiliation: Wenzhou Medical College, Zhejiang Provincial Key Laboratory of Medical Genefics, Wenzhou, Zhejiang, China.
authors.forename: Wen-he
authors.initials: WH
authors.lastname: Wu
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Hong
authors.forename: Xu-wu
authors.initials: XW
authors.lastname: Xiang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Yao
authors.forename: Tin-tin
authors.initials: TT
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Xiong
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Lu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D007477:Ions / Q000032:analysis; D008628:Mercury / Q000032:analysis*; D053768:Metal Nanoparticles; D014867:Water / Q000032:analysis*
chemical_list: D007477:Ions; D014867:Water; D008628:Mercury