title: Dual effects of hydrogen sulphide on focal cerebral ischaemic injury via modulation of oxidative stress-induced apoptosis.
abstract: 1. Hydrogen sulphide (H₂S), one of three signalling gasotransmitters, plays an important role in oxidative stress and apoptosis. However, the effects of H₂S on oxidative stress-induced apoptosis in focal cerebral ischaemic injury in rats have not been clarified. 2. In the present study, sodium hydrosulphide (NaHS) was used as the H₂S donor. Eighty-four Sprague-Dawley rats were randomly divided into six groups: sham, sham + low-dose (2.8 mg/kg) NaHS, sham + high-dose (11.2 mg/kg) NaHS, infarct, infarct + low-dose NaHS and infarct + high-dose NaHS. The focal cerebral ischaemic model was created by cranially inserting a nylon thread with a rounded tip into an internal carotid artery. Rats were killed 21 h after administration of NaHS. 3. In the infarct + low-dose NaHS compared with infarct group, infarct volume was significantly decreased and injury to the mitochondria in nerve cells was mitigated. Furthermore, significant increases were seen in mitochondrial superoxide dismutase and glutathione peroxidase activity and neuronal bcl-2 protein levels, whereas mitochondrial malondialdehyde content and neuronal bax and caspase 3 protein levels were significantly decreased, in the infarct + low-dose NaHS compared with infarct group. The effects seen in the infarct group were significantly aggravated in the infarct + high-dose NaHS group. 4. The findings of the present study provide novel evidence for the dual effects of H₂S on focal cerebral ischaemic injury via modulation of oxidative stress-induced apoptosis.
authors.affiliation: Department of Pharmacology, Hebei Medical University, Shijiazhuang, China.
authors.forename: Guo-Feng
authors.initials: GF
authors.lastname: Li
authors.forename: Hai-Kun
authors.initials: HK
authors.lastname: Luo
authors.forename: Lan-Fang
authors.initials: LF
authors.lastname: Li
authors.forename: Qing-Zeng
authors.initials: QZ
authors.lastname: Zhang
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Xie
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Hao
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Wang
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D020520:Brain Infarction / Q000139:chemically induced / Q000209:etiology / Q000473:pathology / Q000517:prevention & control; D002545:Brain Ischemia / Q000139:chemically induced / Q000209:etiology / Q000503:physiopathology*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D006862:Hydrogen Sulfide / Q000009:adverse effects / Q000627:therapeutic use*; D020244:Infarction, Middle Cerebral Artery / Q000503:physiopathology; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D008933:Mitochondrial Swelling / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects*; D011355:Prodrugs / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*; D013440:Sulfides / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use
chemical_list: D051017:Apoptosis Regulatory Proteins; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D011355:Prodrugs; D013440:Sulfides; C025451:sodium bisulfide; D006862:Hydrogen Sulfide