title: Reduced proliferation and excess astrogenesis of Pax6 heterozygous neural stem/progenitor cells.
abstract: Neural stem/progenitor cells (NSPCs) are generated in early embryonic brains and maintained to produce neurons and glial cells in the central nervous system throughout the lifespan. A transcription factor Pax6 is a pivotal player in various neurodevelopmental processes. Previously, we have shown that Pax6 heterozygous rodents have defects in hippocampal neurogenesis and production of olfactory bulb interneurons. However, characters of NSPCs derived from Pax6 heterozygous rodents have not been studied in vitro. Here we examined the maintenance/proliferation and differentiation of Pax6 heterozygous mutant (rSey(2)/+) rat NSPCs in the neurosphere culture system. We found that the proliferative activity of NSPCs derived from rSey(2)/+ rats was reduced after serial passages. We also observed an excess astrogenesis in serially passaged NSPCs from rSey(2)/+ rats. These results show that Pax6 is essential for maintaining NSPCs and determining their differentiation fates.
authors.affiliation: Division of Developmental Neuroscience, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Sakayori
authors.forename: Takako
authors.initials: T
authors.lastname: Kikkawa
authors.forename: Noriko
authors.initials: N
authors.lastname: Osumi
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000473:pathology*; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured / Q000473:pathology; D002540:Cerebral Cortex / Q000166:cytology; D005136:Eye Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D006579:Heterozygote; D006624:Hippocampus / Q000473:pathology*; D018398:Homeodomain Proteins / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D058953:Neural Stem Cells / Q000378:metabolism / Q000473:pathology*; D009940:Organoids; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors / Q000032:analysis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D016548:Prosencephalon / Q000196:embryology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000032:analysis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
chemical_list: D005136:Eye Proteins; D018398:Homeodomain Proteins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; C000605733:Pax6 protein, rat; D012097:Repressor Proteins