title: Rapid detection of carbapenemase-producing Enterobacteriaceae.
abstract: To rapidly identify carbapenemase producers in Enterobacteriaceae, we developed the Carba NP test. The test uses isolated bacterial colonies and is based on in vitro hydrolysis of a carbapenem, imipenem. It was 100% sensitive and specific compared with molecular-based techniques. This rapid (<2 hours), inexpensive technique may be implemented in any laboratory.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre 78 Rue du Général Leclerc, 94275 K-Bicêtre, France. nordmann.patrice@bct.aphp.fr
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
authors.forename: Laurent
authors.initials: L
authors.lastname: Poirel
authors.forename: Laurent
authors.initials: L
authors.lastname: Dortet
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D001426:Bacterial Proteins / Q000096:biosynthesis*; D001431:Bacteriological Techniques; D015780:Carbapenems / Q000737:chemistry; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D006868:Hydrolysis; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase