title: An iRGD based strategy to study electrochemically the species inside a cell.
abstract: This paper reports a method for electrical communication between the inner part of cells and an electrode with the help of iRGD peptide. Due to the enhancement of the cell penetration caused by iRGD peptide, DNA molecules, previously modified on a gold electrode surface, can be easily transfected into the cells. At the same time, doxorubicin, an anticancer drug, can also be transfected into cells with high penetration. Consequently, doxorubicin binds to DNA chains through electrostatic interaction, and the redox reaction is transferred out of the cell across the cell membrane. As a result, this work may provide a novel way to get information from inside of cells.
authors.affiliation: Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Ning
authors.affiliation: Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Li
authors.affiliation: Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China.|Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.|Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China.
authors.forename: Genxi
authors.initials: G
authors.lastname: Li
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D057846:Cell-Penetrating Peptides / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004317:Doxorubicin / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D015195:Drug Design; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D008297:Male; D008958:Models, Molecular; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000473:pathology; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D000903:Antibiotics, Antineoplastic; D057846:Cell-Penetrating Peptides; D004337:Drug Carriers; C569656:N-end cysteine peptide tumor-homing peptide; D009842:Oligopeptides; D004317:Doxorubicin; D004247:DNA