title: Antigens of worms and eggs showed a differentiated detection of specific IgG according to the time of Schistosoma mansoni infection in mice.
abstract: BACKGROUND
  The correlation between the immunological assay and the antibody titer can offer a tool for the experimental analysis of different phases of the disease.
  
  
  METHODS
  Two simple immunological assays for Schistosoma mansoni in mice sera samples based on specific IgG detection for worms soluble antigens and eggs soluble antigens were standardized and evaluated in our laboratory. Fifty mice were used in negative and positive groups and the results obtained by enzyme-linked immunosorbent assays (ELISA) assays were compared with the number of worms counted and the IgG titers at different times of infection.
  
  
  RESULTS
  Data showed that ELISA using adult worm antigens (ELISA-SWAP) presented a satisfactory correlation between the absorbance value of IgG titers and the individual number of worms counted after perfusion technique (R²=0.62). In addition, ELISA-SWAP differentially detected positive samples with 30 and 60 days post infection (p=0.011 and 0.003, respectively), whereas ELISA using egg antigens (ELISA-SEA) detected samples after 140 days (p=0.03).
  
  
  CONCLUSIONS
  These data show that the use of different antigens in immunological methods can be used as potential tools for the analysis of the chronological evolution of S. mansoni infection in murine schistosomiasis. Correlations with human schistosomiasis are discussed.
authors.affiliation: Laboratório de Esquistossomose, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, MG, Brasil.
authors.forename: Rafaella Fortini Queiroz
authors.initials: RF
authors.lastname: Grenfell
authors.forename: Watson Hermann
authors.initials: WH
authors.lastname: Martins
authors.forename: Vanessa
authors.initials: V
authors.lastname: Silva-Moraes
authors.forename: Suedali Villas-Boas
authors.initials: SV
authors.lastname: Barata
authors.forename: Elizandra Giani
authors.initials: EG
authors.lastname: Ribeiro
authors.forename: Edward
authors.initials: E
authors.lastname: Oliveira
authors.forename: Paulo Marcos Zech
authors.initials: PM
authors.lastname: Coelho
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D010063:Ovum / Q000276:immunology*; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*; D013997:Time Factors
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G