title: Russian "successful" clone B0/W148 of Mycobacterium tuberculosis Beijing genotype: a multiplex PCR assay for rapid detection and global screening.
abstract: We describe a multiplex PCR assay to detect the Mycobacterium tuberculosis Beijing genotype variant B0/W148, which is considered a "successful" clone of M. tuberculosis, widespread in Russia. Specificity and sensitivity of the assay were 100% based on the analysis of a collection of 516 M. tuberculosis isolates of different genotypes and origins. This assay may be used for accurate and simple detection and surveillance of this clinically and epidemiologically important variant of M. tuberculosis.
authors.affiliation: Laboratory of Molecular Microbiology, St. Petersburg Pasteur Institute, St. Petersburg, Russia. igormokrousov@yahoo.com
authors.forename: Igor
authors.initials: I
authors.lastname: Mokrousov
authors.forename: Olga
authors.initials: O
authors.lastname: Narvskaya
authors.forename: Anna
authors.initials: A
authors.lastname: Vyazovaya
authors.forename: Tatiana
authors.initials: T
authors.lastname: Otten
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Jiao
authors.forename: Lia Lima
authors.initials: LL
authors.lastname: Gomes
authors.forename: Philip N
authors.initials: PN
authors.lastname: Suffys
authors.forename: A-Dong
authors.initials: AD
authors.lastname: Shen
authors.forename: Boris
authors.initials: B
authors.lastname: Vishnevsky
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004812:Epidemiologic Methods; D005838:Genotype; D006801:Humans; D008403:Mass Screening / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012426:Russia; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology