title: Lactobacillus porcinae sp. nov., isolated from traditional Vietnamese nem chua.
abstract: A species diversity study of lactic acid bacteria occurring in traditional Vietnamese nem chua yielded an isolate, LMG 26767(T), that could not be assigned to a species with a validly published name. The isolate was initially investigated by 16S rRNA gene sequence analysis, which revealed that it belonged to the genus Lactobacillus, with Lactobacillus manihotivorans and Lactobacillus camelliae as the closest relatives (98.9 % and 96.9 % gene sequence similarity to the type strains, respectively). Comparative (GTG)5-PCR genomic fingerprinting confirmed the unique taxonomic status of the novel strain. DNA-DNA hybridization experiments, DNA G+C content determination, sequence analysis of the phenylalanyl-tRNA synthase (pheS) gene, and physiological and biochemical characterization demonstrated that strain LMG 26767(T) represents a novel species, for which the name Lactobacillus porcinae sp. nov. is proposed; the type strain is LMG 26767(T) ( = CCUG 62266(T)). Biochemically, L. porcinae can be distinguished from L. manihotivorans and L. camelliae by its carbohydrate fermentation profile, absence of growth at 45 °C, and production of d- and l-lactate as end products of glucose metabolism.
authors.affiliation: Department of Biochemistry and Food Biotechnology, Faculty of Food Science and Technology, Hanoi University of Agriculture, Trauquy - Gialam, Hanoi, Vietnam.|Laboratory of Microbiology, Department of Biochemistry and Microbiology, Gent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Doan Thi Lam
authors.initials: DTL
authors.lastname: Nguyen
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Gent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Margo
authors.initials: M
authors.lastname: Cnockaert
authors.affiliation: Faculty of Applied Bioscience Engineering, University College Gent, Schoonmeersstraat 52, 9000 Gent, Belgium.|Laboratory of Microbiology, Department of Biochemistry and Microbiology, Gent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Koenraad
authors.initials: K
authors.lastname: Van Hoorde
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Gent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Evie
authors.initials: E
authors.lastname: De Brandt
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Gent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Isabel
authors.initials: I
authors.lastname: Snauwaert
authors.affiliation: BCCM/LMG Bacteria Collection, Department of Biochemistry and Microbiology, Gent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Cindy
authors.initials: C
authors.lastname: Snauwaert
authors.affiliation: Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: De Vuyst
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam.
authors.forename: Binh Thanh
authors.initials: BT
authors.lastname: Le
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Gent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D005516:Food Microbiology; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008460:Meat / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010652:Phenylalanine-tRNA Ligase / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013552:Swine; D014744:Vietnam
chemical_list: D004269:DNA, Bacterial; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D010652:Phenylalanine-tRNA Ligase