title: Chitooligosaccharides induce apoptosis in human myeloid leukemia HL-60 cells.
abstract: In this study we propose a novel anticancer agent using hetero-chitooligosaccharide (hetero-COS). To examine the possibility of the hetero-COS as a anticancer agent, we prepared nine kinds of hetero-COS with relatively higher molecular weights (90, 75 and 50-COS I, 5-10kDa), medium molecular weights (90, 75 and 50-COS II, 1-5kDa), and lower molecular weights (90, 75 and 50-III, below 1kDa), and their anticancer properties were investigated on HL-60 cells using flow cytometry and morphological analysis. The results obtained indicate that 90-COS III, which is relatively higher degree of deacetylation and lower molecular weights, showed the highest anticancer activity, and the data showed the anticancer property of the hetero-COSs depended on their degree of deacetylation values and molecular weight.
authors.affiliation: Department of Food Life Science, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Je
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.forename: Si-Heung
authors.initials: SH
authors.lastname: Sung
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002236:Carbohydrate Conformation; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D018922:HL-60 Cells; D006801:Humans; D007951:Leukemia, Myeloid / Q000188:drug therapy* / Q000473:pathology; D008970:Molecular Weight; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D009844:Oligosaccharides