title: Optimizing nanovial outlet designs for improved solid-phase extraction in the integrated selective enrichment target--ISET.
abstract: The integrated selective enrichment target is a microfluidic platform for SPE sample preparation with integrated nanocolumns, which simultaneously offers direct MALDI MS read-out. Here, we present a study on the importance of different nanocolumn outlet hole geometries and hole areas in relation to MS signal intensity and reproducibility. A design solution that provides the flow characteristics required for robust sample preparation using automated liquid handling is reported.
authors.affiliation: Division of Nanotechnology, Department of Measurement Technology and Industrial Electrical Engineering, Lund University, Lund, Sweden.
authors.forename: Belinda
authors.initials: B
authors.lastname: Adler
authors.forename: Thomas
authors.initials: T
authors.lastname: Laurell
authors.forename: Simon
authors.initials: S
authors.lastname: Ekström
mesh_terms: D004867:Equipment Design; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D040901:Proteomics / Q000295:instrumentation*; D052616:Solid Phase Extraction / Q000295:instrumentation*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation*