title: Predictive potential of glutathione S-transferase polymorphisms for prognosis of osteosarcoma patients on chemotherapy.
abstract: OBJECTIVE
  To evaluate the predictive value of glutathione S-transferase (GST) gene polymorphisms for the prognosis of osteosarcoma patients receiving chemotherapy.
  
  
  METHODS
  A total of 159 patients were included in our study between January 2005 and December 2007., with follow-up until January 2012. Genotyping was based upon the duplex polymerase-chain-reaction with the PCR-CTPP method.
  
  
  RESULTS
  At the time of diagnosis, 15.4% of the patients presented with metastasis, while 22.3% developed metastasis during follow-up. At the time of final analysis on January 2012, the median follow-up was 45.5 months. Patients with null GSTM1 and GSTT1 had a higher event free survival rate than non-null genotype, but no significant association was found between the two genotypes and prognosis of osteosarcoma. Individuals with GSTP1 Val/Val genotype tended to live shorter than with the IIe/IIe genotype, and we found a significantly higher risk of death from osteosarcoma (adjusted HR=2.35, 95% CI=1.13-4.85).
  
  
  CONCLUSIONS
  The GSTP1 gene polymorphism may have an important role in the prognosis of osteosarcoma patients with chemotherapy. Further analyses with larger samples and more genes encoding metabolizing and DNA repair enzymes are warranted.
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Shai-Lin
authors.initials: SL
authors.lastname: Zhang
authors.forename: Ning-Fang
authors.initials: NF
authors.lastname: Mao
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Sun
authors.forename: Zhi-Cai
authors.initials: ZC
authors.lastname: Shi
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Sun
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000235:genetics / Q000401:mortality; D002648:Child; D018572:Disease-Free Survival; D005260:Female; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000188:drug therapy* / Q000201:enzymology* / Q000235:genetics / Q000401:mortality; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1