title: Localized delivery of dexamethasone from electrospun fibers reduces the foreign body response.
abstract: Synthetic scaffolds are crucial to applications in regenerative medicine; however, the foreign body response can impede regeneration and may lead to failure of the implant. Herein we report the development of a tissue engineering scaffold that allows attachment and proliferation of regenerating cells while reducing the foreign body response by localized delivery of an anti-inflammatory agent. Electrospun fibers composed of poly(l-lactic) acid (PLLA) and poly(ε-caprolactone) (PCL) were prepared with and without the steroid anti-inflammatory drug, dexamethasone. Analysis of subcutaneous implants demonstrated that the PLLA fibers encapsulating dexamethasone evoked a less severe inflammatory response than the other fibers examined. They also displayed a controlled release of dexamethasone over a period of time conducive to tissue regeneration and allowed human mesenchymal stem cells to adhere to and proliferate on them in vitro. These observations demonstrate their potential as a building block for tissue engineering scaffolds.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology , Cambridge, MA, USA.
authors.forename: Nathaniel M
authors.initials: NM
authors.lastname: Vacanti
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.forename: Paulina S
authors.initials: PS
authors.lastname: Hill
authors.forename: João D T
authors.initials: JD
authors.lastname: Guerreiro
authors.forename: Tram T
authors.initials: TT
authors.lastname: Dang
authors.forename: Minglin
authors.initials: M
authors.lastname: Ma
authors.forename: Shanée
authors.initials: S
authors.lastname: Watson
authors.forename: Nathaniel S
authors.initials: NS
authors.lastname: Hwang
authors.forename: Robert
authors.initials: R
authors.lastname: Langer
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Anderson
mesh_terms: D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000494:pharmacology* / Q000627:therapeutic use; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D003907:Dexamethasone / Q000008:administration & dosage* / Q000494:pharmacology* / Q000627:therapeutic use; D005549:Foreign-Body Reaction / Q000188:drug therapy / Q000517:prevention & control*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods
chemical_list: D000893:Anti-Inflammatory Agents; D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D019344:Lactic Acid; C033616:poly(lactide); D003907:Dexamethasone