title: Hypothalamic transcript profiling in hypothermia using SuperSAGE.
abstract: Understanding of molecular mechanisms underlying hypothermia is of primary importance in devising strategies to diagnose hypothermia. We investigated the hypothalamic transriptome in hypothermia. For transcriptomic analyses, SuperSAGE, an improved method of serial analysis of gene expression, was used. Totally, 62,208 and 54,084 tags were collected from the hypothalami of normal and hypothermia, respectively. And 367 transcripts were differentially expressed at a statistically significant level. That is, 157 and 210 transcripts among them were expressed at a higher level in normal and hypothermic hypothalami. Results obtained by SuperSAGE and quantitative PCR were consistent in 6 selected genes, although levels of differences detected by the 2 methods were not exactly the same. mRNA expressions in the hypothalamus were considered to be useful for hypothermic diagnosis. Various methods have been applied for gene expression analyses and biomarker detections. However in forensic pathology, SuperSAGE would be a promising method, especially in gene discoveries and transcriptomic analyses.
authors.affiliation: Department of Legal Medicine, Iwate Medical University, 2-1-1 Nishitokuta, Yahaba, Iwate 028-3694, Japan. mtakamiy@iwate-med.ac.jp
authors.forename: Masataka
authors.initials: M
authors.lastname: Takamiya
authors.forename: Nori
authors.initials: N
authors.lastname: Nakayashiki
authors.forename: Koji
authors.initials: K
authors.lastname: Dewa
mesh_terms: D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D020224:Expressed Sequence Tags; D053803:Forensic Genetics; D049429:Forensic Pathology; D020869:Gene Expression Profiling; D050883:HSC70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism*; D007035:Hypothermia / Q000175:diagnosis*; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D059467:Transcriptome; D014365:Tryptophan Hydroxylase / Q000235:genetics / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism
chemical_list: D002148:Calmodulin-Binding Proteins; D002868:Chromosomal Proteins, Non-Histone; D050483:Dopamine Plasma Membrane Transport Proteins; D050883:HSC70 Heat-Shock Proteins; C494373:Hspa8 protein, rat; D009419:Nerve Tissue Proteins; C489090:Pcp4 protein, rat; D012333:RNA, Messenger; C489913:Rtn1 protein, rat; C493216:Slc6a3 protein, rat; C459407:Zwint protein, rat; D014446:Tyrosine 3-Monooxygenase; D014365:Tryptophan Hydroxylase