title: IL-6 promotes ICAM-1 expression and cell motility in human osteosarcoma.
abstract: Osteosarcoma is characterized by a high malignant and metastatic potential. Interleukin-6 (IL-6) is a multifunctional cytokine that is associated with the disease status and outcomes of cancers. However, the effect of IL-6 on migration activity in human osteosarcoma cells is mostly unknown. Here we found that IL-6 increased the migration and expression of intercellular adhesion molecule-1 (ICAM-1) in human osteosarcoma cells. Transfection of cells with ICAM-1 siRNA reduced IL-6-mediated cell migration. We also found that expression of IL-6 was significantly greater in human osteosarcoma tissues than in normal bone. The integrin-linked kinase (ILK)/Akt/AP-1 pathway was activated after IL-6 treatment, and IL-6-induced expression of ICAM-1 and migration activity was inhibited by the specific inhibitor and siRNA of ILK, Akt, and AP-1 cascades. In addition, over-expression of IL-6 shRNA inhibited the migratory ability and ICAM-1 expression in osteosarcoma cells. Taken together, these results indicate that IL-6 and IL-6 receptor interaction occurs through ILK and Akt, which in turn activates AP-1, resulting in the activations of ICAM-1 and contributing the migration of human osteosarcoma cells.
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Lin
authors.forename: Zi-Ling
authors.initials: ZL
authors.lastname: Chang
authors.forename: Yuan-Ya
authors.initials: YY
authors.lastname: Liao
authors.forename: Ming-Chih
authors.initials: MC
authors.lastname: Chou
authors.forename: Chih-Hsin
authors.initials: CH
authors.lastname: Tang
mesh_terms: D002465:Cell Movement / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism*; D019947:Receptors, Interleukin-6 / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D015850:Interleukin-6; D019947:Receptors, Interleukin-6; D018799:Intercellular Adhesion Molecule-1