title: The influence of 8-prenylnaringenin on the activity of voltage-gated Kv1.3 potassium channels in human Jurkat T cells.
abstract: Using the whole-cell patch-clamp technique, we investigated the influence of 8-prenylnaringenin on the activity of the voltage-gated Kv1.3 potassium channels in the human leukemic T lymphocyte cell line Jurkat. 8-prenylnaringenin is a potent plant-derived phytoestrogen that has been found to inhibit cancer cell proliferation. The results show that it inhibited the Kv1.3 channels in a concentration-dependent manner. Complete inhibition occurred at concentrations higher than 10 μM. The inhibitory effect of 8-prenylnaringenin was reversible. It was accompanied by a significant acceleration of channel inactivation without any pronounced change in the activation rate. Of the naringenin derivatives tested to date, 8-prenylnaringenin is the most potent inhibitor of the Kv1.3 channels. The potency of the inhibition may be due to the presence of a prenyl group in the molecule of this flavonoid. The inhibition of the Kv1.3 channels might be involved in the antiproliferative and pro-apoptotic effects of 8-prenylnaringenin that have been observed in cancer cell lines expressing these channels.
authors.affiliation: Department of Biophysics, Wrocław Medical University, Wrocław, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Gąsiorowska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Teisseyre
authors.forename: Anna
authors.initials: A
authors.lastname: Uryga
authors.forename: Krystyna
authors.initials: K
authors.lastname: Michalak
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D044950:Flavanones / Q000494:pharmacology*; D006801:Humans; D019169:Jurkat Cells; D051664:Kv1.3 Potassium Channel / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018408:Patch-Clamp Techniques
chemical_list: C119737:8-prenylnaringenin; D044950:Flavanones; D051664:Kv1.3 Potassium Channel