title: Morphological and molecular characterization of Ortleppascaris sp. larvae, parasites of the cane toad Rhinella marina from eastern Amazonia.
abstract: This study presents a new record for the occurrence of larval Ortleppascaris sp.(Sprent, 1978). The nematodes were collected from inside fibrous cysts found in the livers of cane toads, Rhinella marina (Linnaeus, 1758), captured in eastern Brazilian Amazonia. This is the first record of Ortleppascaris sp. larvae in both Brazil and this amphibian host, increasing its distribution in South America as well as expanding the number of helminths known to infect this toad. The detailed description of Ortleppascaris sp. provides new taxonomic data for these larvae, as well as sequences of the internal transcribed spacers and small subunit DNA segments, and the cytochrome oxidase I gene, which will, in time, contribute to a better understanding of the phylogeny of this group of parasites.
authors.affiliation: Laboratório de Biologia Celular e Helmintologia Profa. Dra. Reinalda Marisa Lanfredi, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
authors.forename: Jefferson P
authors.initials: JP
authors.lastname: e Silva
authors.forename: Francisco T V
authors.initials: FT
authors.lastname: Melo
authors.forename: Luciana C N
authors.initials: LC
authors.lastname: Silva
authors.forename: Evonnildo C
authors.initials: EC
authors.lastname: Gonçalves
authors.forename: Elane G
authors.initials: EG
authors.lastname: Giese
authors.forename: Adriano P
authors.initials: AP
authors.lastname: Furtado
authors.forename: Jeannie N
authors.initials: JN
authors.lastname: Santos
mesh_terms: D000818:Animals; D017191:Ascaridida Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D001199:Ascaridoidea / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics / Q000648:ultrastructure; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002025:Bufonidae / Q000469:parasitology*; D018509:DNA, Helminth / Q000737:chemistry; D021901:DNA, Intergenic / Q000737:chemistry; D007814:Larva / Q000033:anatomy & histology / Q000145:classification / Q000235:genetics / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D008969:Molecular Sequence Data; D015995:Prevalence
chemical_list: D018509:DNA, Helminth; D021901:DNA, Intergenic