title: Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy             of SW480 cells.
abstract: A proliferation-inducing ligand (APRIL) is a key factor involved in the             tumor development and progression in some tumor tissues and cells. Its overexpression             and as gene target in SW480 colon carcinoma cells was confirmed in our previous             study. To seek a more potent way to treat colon carcinoma using a gene therapy             method, herein, we constructed a multiple short hairpin RNA (shRNA) expression             vector containing four shRNAs against the APRIL gene in SW480 cells. APRIL expression             levels and cell biological behavior were detected after transfection with different             kinds of vectors. As expected, we found that our multiple shRNA vector produced             a more significant knockdown effect of APRIL than the vectors containing only             one APRIL shRNA. Furthermore, our findings indicate that silencing APRIL expression             in SW480 cells decreased their malignancy by reducing proliferation, invasion             and adhesion, as well as inducing apoptosis. Based on our findings, vectors containing             multiple shRNAs to silence the expression of APRIL may be exploited as a novel             therapeutic strategy for tumors.
authors.affiliation: Department of Clinical Laboratory Center, Affiliated Hospital of Nantong University, Nantong Medical College, Nantong University, Nantong 226001, PR China.
authors.forename: Haiquan
authors.initials: H
authors.lastname: Li
authors.forename: Keke
authors.initials: K
authors.lastname: Shao
authors.forename: Jingchun
authors.initials: J
authors.lastname: Wang
authors.forename: Guihua
authors.initials: G
authors.lastname: Wang
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Jinde
authors.initials: J
authors.lastname: Cao
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Ju
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D006801:Humans; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D034741:RNA, Small Interfering; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D034741:RNA, Small Interfering; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13