title: Total thiol redox status as a potent biomarker of PAH-mediated effects on mussels.
abstract: This laboratory study describes phenanthrene (Ph) and/or anthracene (An) ability to alter the total thiol redox status (TRS), via depletion of protein free thiols (PSH) and glutathione (GSH) levels, in gills of mussel Mytilus galloprovincialis, after a short-term (7 days) exposure period to each contaminant (at a final concentration of 0.1 mg L(-1)) or in a mixture (ration 1:1, at a final concentration of 0.2 mg L(-1)). A number of observable changes, like lysosomal membrane impairment (as detected via the neutral red retention time assay, primarily performed in haemocytes), enhancement of lipid peroxidation byproducts, increased nuclear abnormalities, inhibition of AChE and ALP activity, as well as a significant depletion of PSH and GSH were detected in gills of exposed mussels, in any case. Significant relationships occurred among TRS parameters with each change/stress indices measured in tissues of mussels, could reinforce the use of PSH as a potent biomarker.
authors.affiliation: Department of Biology, Section of Genetics, Cell Biology and Development, University of Patras, Patras, Greece.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Grintzalis
authors.forename: Christos D
authors.initials: CD
authors.lastname: Georgiou
authors.forename: Stefanos
authors.initials: S
authors.lastname: Dailianis
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D005880:Gills / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006434:Hemocytes / Q000187:drug effects / Q000378:metabolism; D015162:Micronucleus Tests; D049878:Mytilus / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D011084:Polycyclic Aromatic Hydrocarbons / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000032:analysis / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D013438:Sulfhydryl Compounds; D014874:Water Pollutants, Chemical; D005978:Glutathione