title: Controlling the localization of polymer-functionalized nanoparticles in mixed lipid/polymer membranes.
abstract: Surface hydrophobicity plays a significant role in controlling the interactions between nanoparticles and lipid membranes. In principle, a nanoparticle can be encapsulated into a liposome, either being incorporated into the hydrophobic bilayer interior or trapped within the aqueous vesicle core. In this paper, we demonstrate the preparation and characterization of polymer-functionalized CdSe NPs, tuning their interaction with mixed lipid/polymer membranes from 1,2-dipalmitoyl-sn-glycero-3-phophocholine and PIB(87)-b-PEO(17) block copolymer by varying their surface hydrophobicity. It is observed that hydrophobic PIB-modified CdSe NPs can be selectively located within polymer domains in a mixed lipid/polymer monolayer at the air/water interface, changing their typical domain morphologies, while amphiphilic PIB-PEO-modified CdSe NPs showed no specific localization in phase-separated lipid/polymer films. In addition, hydrophilic water-soluble CdSe NPs can readily adsorb onto spread monolayers, showing a larger effect on the molecule packing at the air/water interface in the case of pure lipid films compared to mixed monolayers. Furthermore, the incorporation of PIB-modified CdSe NPs into hybrid lipid/polymer GUVs is demonstrated with respect to the prevailing phase state of the hybrid membrane. Monitoring fluorescent-labeled PIB-CdSe NPs embedded into phase-separated vesicles, it is demonstrated that they are enriched in one specific phase, thus probing their selective incorporation into the hydrophobic portion of PIB(87)-b-PEO(17) BCP-rich domains. Thus, the formation of biocompatible hybrid GUVs with selectively incorporated nanoparticles opens a new perspective for subtle engineering of membranes together with their (nano-) phase structure serving as a model system in designing functional nanomaterials for effective nanomedicine or drug delivery.
authors.affiliation: Macromolecular Chemistry, Institute of Chemistry, Faculty of Natural Sciences II (Chemistry, Physics and Mathematics), Martin-Luther University Halle-Wittenberg, D-06120 Halle (Saale), Germany.
authors.forename: Adekunle
authors.initials: A
authors.lastname: Olubummo
authors.forename: Matthias
authors.initials: M
authors.lastname: Schulz
authors.forename: Bob-Dan
authors.initials: BD
authors.lastname: Lechner
authors.forename: Peggy
authors.initials: P
authors.lastname: Scholtysek
authors.forename: Kirsten
authors.initials: K
authors.lastname: Bacia
authors.forename: Alfred
authors.initials: A
authors.lastname: Blume
authors.forename: Jörg
authors.initials: J
authors.lastname: Kressler
authors.forename: Wolfgang H
authors.initials: WH
authors.lastname: Binder
mesh_terms: D003460:Crystallization / Q000379:methods*; D008055:Lipids / Q000737:chemistry*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D008567:Membranes, Artificial; D008968:Molecular Conformation; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008055:Lipids; D046911:Macromolecular Substances; D008567:Membranes, Artificial; D011108:Polymers