title: Production and characterization of acidophilic xylanolytic enzymes from Penicillium oxalicum GZ-2.
abstract: Multiple acidophilic xylanolytic enzymes were produced by Penicillium oxalicum GZ-2 during growth on wheat straw, rice straw, corn stover, and wheat bran. The expression of xylanase isoforms was dependent on substrate type and nitrogen source. The zymograms produced by the SDS-PAGE resolution of the crude enzymes indicated that wheat straw was the best inducer, resulting in the highest xylanase (115.2U/mL) and β-xylosidase (89mU/mL) activities during submerged fermentation. The optimum temperature and pH for xylanase activity were 50°C and 4.0, respectively; however, the crude xylanase enzymes exhibited remarkable stability over a broad pH range and showed more than 90% activity at 50°C for 30min at pH 4.0-8.0. The results revealed that P. oxalicum GZ-2 is a promising acidophilic xylanase-producing microorganism that has great potential to be used in biofuels, animal feed, and food industry applications.
authors.affiliation: Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hanpeng
authors.initials: H
authors.lastname: Liao
authors.forename: Chunmiao
authors.initials: C
authors.lastname: Xu
authors.forename: Shiyong
authors.initials: S
authors.lastname: Tan
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wei
authors.forename: Ning
authors.initials: N
authors.lastname: Ling
authors.forename: Guanghui
authors.initials: G
authors.lastname: Yu
authors.forename: Waseem
authors.initials: W
authors.lastname: Raza
authors.forename: Ruifu
authors.initials: R
authors.lastname: Zhang
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
authors.forename: Yangchun
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000143:Acids / Q000378:metabolism*; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000494:pharmacology; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D004795:Enzyme Stability / Q000187:drug effects; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000494:pharmacology; D010407:Penicillium / Q000187:drug effects / Q000201:enzymology*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D013696:Temperature; D013997:Time Factors; D014990:Xylans / Q000378:metabolism*
chemical_list: D000143:Acids; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; D014990:Xylans; D002244:Carbon; D043364:Endo-1,4-beta Xylanases; D009584:Nitrogen