title: Crystallographic study of the interaction of the anti-HIV lectin actinohivin with the α(1-2)mannobiose moiety of gp120 HMTG.
abstract: Actinohivin (AH) is a new potent anti-HIV lectin of microbial origin. In order to modify it to produce a more efficient drug, its three-dimensional structure has previously been determined with and without the target α(1-2)mannobiose moiety of the high-mannose-type glycan (HMTG) attached to HIV-1 gp120. However, ambiguity remained in the structures owing to packing disorder that was possibly associated with peptide fragments attached at the N-terminus. To resolve these problems, the duration of cultivation of the AH-producing strain was examined and it was found that in a sample obtained from a 20 d culture the heterogeneous fragments were completely removed to produce mature AH with high homogeneity. In addition, the purification procedures were simplified in order to increase the yield of AH and the addition of solvents was also examined in order to increase the solubility of AH. AH thus obtained was successfully crystallized with high reproducibility in a different form to the previously obtained crystals. The crystal diffracted well to beyond 1.90 Å resolution and the crystallographic data suggested that it contained no packing disorder.
authors.affiliation: College of Science and Engineering, Iwaki Meisei University, Iwaki, Fukushima 970-8551, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Suzuki
authors.forename: Naomi
authors.initials: N
authors.lastname: Ohbayashi
authors.forename: Jiandong
authors.initials: J
authors.lastname: Jiang
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Zhang
authors.forename: M Mominul
authors.initials: MM
authors.lastname: Hoque
authors.forename: Masaru
authors.initials: M
authors.lastname: Tsunoda
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Murayama
authors.forename: Haruo
authors.initials: H
authors.lastname: Tanaka
authors.forename: Akio
authors.initials: A
authors.lastname: Takénaka
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000378:metabolism; D015497:HIV-1 / Q000737:chemistry* / Q000378:metabolism; D008358:Mannose / Q000737:chemistry* / Q000378:metabolism; D008848:Micromonosporaceae / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding
chemical_list: D001426:Bacterial Proteins; D015699:HIV Envelope Protein gp120; C424136:actinohivin protein, actinomycete; D008358:Mannose