title: Mass spectrometry based proteomics, background, status and future needs.
abstract: An overview of the background for proteomics and a description of the present state of art are given with a description of the main strategies in proteomics. The advantages and limitations of the two major strategies, 2D-gel based and LC-MS based, are discussed and a combination for the two, CeLC-MS is described. A number of challenging problems which have been solved using different proteomics strategies including the advantage of organell enrichment or modifications specific peptide isolation to get deeper into the proteome are described. Finally the present status and future needs discussed.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230, Odense M, Denmark. roe@bmb.sdu.dk
authors.forename: Peter
authors.initials: P
authors.lastname: Roepstorff
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D010766:Phosphorylation; D020033:Protein Isoforms / Q000032:analysis; D020543:Proteome / Q000032:analysis; D040901:Proteomics
chemical_list: D010455:Peptides; D020033:Protein Isoforms; D020543:Proteome