title: Thermo-switchable antibacterial activity.
abstract: A copolymer system of 2-aminoethyl methacrylate and N-isopropylacrylamide comprises novel properties: changes in conformation and hydrophilicity upon heating influence the antibacterial activity and result in a switchable biocidal effect. The copolymers are characterized via NMR, MALDI-ToF MS, phase transition behavior, and antibacterial tests with E. coli or B. subtilis. MIC and MBC are determined using standard dilution methods, temperature-dependence via incubation at different temperatures and cytotoxicity by MTT tests. The copolymers exhibit lower MIC in globule than coil conformation, crosslinking on cotton results in non-leaching materials with better antibacterial activity above than below the phase-transition point.
authors.affiliation: Department of Chemistry and Scientific Center for Materials Science, Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35042 Marburg, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Mattheis
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Seema
authors.initials: S
authors.lastname: Agarwal
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects* / Q000254:growth & development; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008968:Molecular Conformation; D044367:Phase Transition; D011109:Polymethacrylic Acids / Q000138:chemical synthesis* / Q000494:pharmacology; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D011109:Polymethacrylic Acids; C578452:poly(2-aminoethyl methacrylate-co-N-isopropylacrylamide)