title: Preparation of dry extract of Mikania glomerata Sprengel (Guaco) and determination of its coumarin levels by spectrophotometry and HPLC-UV.
abstract: Guaco (Mikania glomerata Sprengel) syrup is one of the most popular herbal medicines used to treat the symptoms of asthmatic bronchitis, cough and hoarseness. The coumarin 2H-1-benzopyran-2-one, is one of the major constituents of Guaco and contributes to its pharmacological effects. The pharmaceutical capsule form of dry extract of Guaco is recommended by the Brazilian Program of Medicinal Plants and Herbal Medicines and used in primary health care. In order to identify a new protocol to obtain the raw material for Guaco capsule production we evaluated two methods, including a freezedrying process (lyophilization) and the spray-dryer technique, as well as the use of two adjuvants, Maltodextrins and Aerosil®, in different concentrations. The coumarin levels of the dried extracts were analyzed by UV-spectrophotometry and HPLC-UV/DAD. The adjuvant Aerosil® 8% showed better dry powder physical appearance. Lyophilization was observed to be the best process to obtain the dry extract of Guaco based on the measured coumarin levels.
authors.affiliation: Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil. luciana.soaresesilva@gmail.com
authors.forename: Luciana Soares E
authors.initials: LS
authors.lastname: Soares e Silva
authors.forename: Luciane Santos
authors.initials: LS
authors.lastname: da Santos da Silva
authors.forename: Larissa
authors.initials: L
authors.lastname: Brumano
authors.forename: Paulo César
authors.initials: PC
authors.lastname: Stringheta
authors.forename: Miriam
authors.initials: M
authors.lastname: Aparecida de Oliveira Pinto
authors.forename: Leticia Oliveira
authors.initials: LO
authors.lastname: Moreira Dias
authors.forename: Camila
authors.initials: C
authors.lastname: de Sá Martins Muller
authors.forename: Elita
authors.initials: E
authors.lastname: Scio
authors.forename: Rodrigo Luiz
authors.initials: RL
authors.lastname: Fabri
authors.forename: Helena C
authors.initials: HC
authors.lastname: Castro
authors.forename: Maria
authors.initials: M
authors.lastname: da Penha Henriques do Amaral
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis*; D005612:Freeze Drying; D035742:Mikania / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D013053:Spectrophotometry
chemical_list: D003374:Coumarins; D010936:Plant Extracts; C030123:coumarin