title: Preparation and evaluation of warfarin-β-cyclodextrin loaded chitosan nanoparticles for transdermal delivery.
abstract: The main objective of the present work was to prepare warfarin-β-cyclodextrin (WAF-β-CD) loaded chitosan (CS) nanoparticles for transdermal delivery. CS is a hydrophilic carrier therefore, to overcome the hydrophobic nature of WAF and allow its incorporation into CS nanoparticles, WAF was first complexed with β-cyclodextrin (β-CD). CS nanoparticles were prepared by ionotropic pre-gelation using tripolyphosphate (TPP). Morphology, size and structure characterization of nanoparticles were carried out using SEM, TEM and FTIR, respectively. Nanoparticles prepared with 3:1 CS:TPP weight ratio and 2mg/ml final CS concentration were found optimum. They possessed spherical particles (35±12nm diameter) with narrow size distribution (PDI=0.364) and 94% entrapment efficiency. The in vitro release as well as the ex vivo permeation profiles of WAF-β-CD from the selected nanoparticle formulation were studied at different time intervals up to 8h. In vitro release of WAF-β-CD from CS nanoparticles followed a Higuchi release profile whereas its ex vivo permeation (at pH 7.4) followed a zero order permeation profile. Results suggested that the developed WAF-β-CD loaded CS carrier could offer a controlled and constant delivery of WAF transdermally.
authors.affiliation: Advanced Materials and Nanotechnology Group, Center of Excellence for Advanced Sciences, Department of Spectroscopy, Division of Physics, National Research Center, Cairo, Egypt. safaakhk@yahoo.com
authors.forename: Safaa K H
authors.initials: SK
authors.lastname: Khalil
authors.forename: Gina S
authors.initials: GS
authors.lastname: El-Feky
authors.forename: Sally T
authors.initials: ST
authors.lastname: El-Banna
authors.forename: Wafaa A
authors.initials: WA
authors.lastname: Khalil
mesh_terms: D000279:Administration, Cutaneous; D000925:Anticoagulants / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D014859:Warfarin / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000925:Anticoagulants; D004337:Drug Carriers; D047392:beta-Cyclodextrins; D014859:Warfarin; D048271:Chitosan