title: Effect of coffee combining green coffee bean constituents with typical roasting products on the Nrf2/ARE pathway in vitro and in vivo.
abstract: This study investigated Nrf2-activating properties of a coffee blend combining raw coffee bean constituents with 5-O-caffeoylquinic acid (CGA) as a lead component with typical roasting products such as N-methylpyridinium (NMP). In cell culture (HT29) the respective coffee extract (CN-CE) increased nuclear Nrf2 translocation and enhanced the transcription of ARE-dependent genes as exemplified for NAD(P)H:quinone oxidoreductase and glutathione-S-transferase (GST)A1, reflected in the protein level by an increase in GST enzyme activity. In a pilot human intervention study (29 healthy volunteers), daily consumption of 750 mL of CN-coffee for 4 weeks increased Nrf2 transcription in peripheral blood lymphocytes on average. However, the transcriptional response pattern of Nrf2/ARE-dependent genes showed substantial interindividual variations. The presence of SNPs in the Nrf2-promoter, reported recently, as well as the detection of GSTT1*0 (null) genotypes in the study collective strengthens the hypothesis that coffee acts as a modulator of Nrf2-dependent gene response in humans, but genetic polymorphisms play an important role in the individual response pattern.
authors.affiliation: Department of Food Chemistry and Toxicology, University of Vienna, Währingerstrasse 38, A-1090 Vienna, Austria.
authors.forename: Nadine
authors.initials: N
authors.lastname: Volz
authors.forename: Ute
authors.initials: U
authors.lastname: Boettler
authors.forename: Swantje
authors.initials: S
authors.lastname: Winkler
authors.forename: Nicole
authors.initials: N
authors.lastname: Teller
authors.forename: Christoph
authors.initials: C
authors.lastname: Schwarz
authors.forename: Tamara
authors.initials: T
authors.lastname: Bakuradze
authors.forename: Gerhard
authors.initials: G
authors.lastname: Eisenbrand
authors.forename: Larissa
authors.initials: L
authors.lastname: Haupt
authors.forename: Lyn R
authors.initials: LR
authors.lastname: Griffiths
authors.forename: Herbert
authors.initials: H
authors.lastname: Stiebitz
authors.forename: Gerhard
authors.initials: G
authors.lastname: Bytof
authors.forename: Ingo
authors.initials: I
authors.lastname: Lantz
authors.forename: Roman
authors.initials: R
authors.lastname: Lang
authors.forename: Thomas
authors.initials: T
authors.lastname: Hofmann
authors.forename: Veronika
authors.initials: V
authors.lastname: Somoza
authors.forename: Doris
authors.initials: D
authors.lastname: Marko
mesh_terms: D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D003069:Coffee / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D005982:Glutathione Transferase / Q000235:genetics; D019073:HT29 Cells / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics; D051267:NF-E2-Related Factor 2 / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D021381:Protein Transport / Q000187:drug effects; D011726:Pyridinium Compounds / Q000494:pharmacology*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D020218:Response Elements
chemical_list: D002726:Chlorogenic Acid; D003069:Coffee; D005982:Glutathione Transferase; D016660:NAD(P)H Dehydrogenase (Quinone); D051267:NF-E2-Related Factor 2; D011726:Pyridinium Compounds; D011801:Quinic Acid; C495635:NFE2L2 protein, human; C014677:1-methylpyridinium; C447479:NQO1 protein, human; C413545:glutathione S-transferase T1; C484555:GSTA1 protein, human; C473200:5-caffeoylquinic acid