title: CXCL12 improves immune responses to neurotropic virus propagation in the CNS by attracting antibody secreting cells.
abstract: A previous study showed that increases in chemokine expression and recruitment of antibody secreting cells (ASCs) in the CNS after intracerebral immunization contributed to the suppression of a neurotropic virus. In this study, intracerebral chemokine injection was used to investigate the usefulness of chemokines for controlling neurotropic viruses. Both CXCL12 and a cocktail chemokine (a mixture of CXCL9, 10, 12 and 13) attracted antigen-specific ASCs more strongly than CXCL9, 10 and 13 in an in vitro chemotaxis assay and in vivo intracerebral chemokine injection experiments. Mice pre-treated intracerebrally with CXCL12 and the cocktail chemokine showed an increased survival rate after intracerebral infection with rabies virus. These results suggest that intracerebral CXCL12 injection induces the migration of ASCs and suppresses the neuropathogenicity of rabies virus in the CNS.
authors.affiliation: Laboratory of Comparative Pathology, Graduate School of Veterinary Medicine, Hokkaido University, N18 W9, Sapporo 060-0818, Japan.
authors.forename: Hyunkyoung
authors.initials: H
authors.lastname: Lee
authors.forename: Yuji
authors.initials: Y
authors.lastname: Sunden
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sakai
authors.forename: Kenji
authors.initials: K
authors.lastname: Ochiai
authors.forename: Takashi
authors.initials: T
authors.lastname: Umemura
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000276:immunology* / Q000821:virology; D054377:Chemokine CXCL12 / Q000276:immunology / Q000494:pharmacology*; D002633:Chemotaxis / Q000276:immunology; D005260:Female; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D011557:Pseudorabies / Q000276:immunology* / Q000821:virology; D011818:Rabies / Q000276:immunology* / Q000821:virology; D011820:Rabies virus / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D018709:Statistics, Nonparametric
chemical_list: D054377:Chemokine CXCL12; D007136:Immunoglobulins