title: Soluble ST2 protein inhibits LPS stimulation on monocyte-derived dendritic cells.
abstract: ST2 protein is a soluble splicing variant of ST2L protein, which is the receptor for interleukin-33 (IL-33). Previously, we reported that soluble ST2 suppressed the signal transduction of lipopolysaccharide (LPS) and cytokine production in monocytic cells. To investigate whether or not this inhibitory effect occurs in dendritic cells, which are the key players in innate and adaptive immunity, human monocyte-derived dendritic cells were pre-treated with soluble ST2 protein before LPS stimulation. Although soluble ST2 did not attenuate the LPS-induced maturation of dendritic cells, pre-treatment with soluble ST2 suppressed cytokine production and inhibited LPS signaling. Moreover, the proliferation of naive T cells was inhibited significantly by soluble ST2 pre-treatment. IL-33 had little effect on the cytokine production of immature monocyte-derived dendritic cells. Furthermore, soluble ST2 protein was internalized into dendritic cells, suggesting that soluble ST2 protein acts by a noncanonical mechanism other than the sequestration of IL-33.
authors.affiliation: Department of Biochemistry, School of Medicine, Jichi Medical University, Shimotsuke-shi, Tochigi, Japan.
authors.forename: Akihisa
authors.initials: A
authors.lastname: Nagata
authors.forename: Naoki
authors.initials: N
authors.lastname: Takezako
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tamemoto
authors.forename: Hiromi
authors.initials: H
authors.lastname: Ohto-Ozaki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohta
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Tominaga
authors.forename: Ken
authors.initials: K
authors.lastname: Yanagisawa
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D007378:Interleukins / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009000:Monocytes / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D021381:Protein Transport; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D016207:Cytokines; C079241:IL1RL1 protein, human; C506536:IL33 protein, human; D000072179:Interleukin-1 Receptor-Like 1 Protein; D000067596:Interleukin-33; D007378:Interleukins; D008070:Lipopolysaccharides; D011956:Receptors, Cell Surface