title: Validated LC-MS-MS method for the determination of prodrug of ginkgolide B in rat plasma and brain: application to pharmacokinetic study.
abstract: A sensitive, simple and rapid liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of ginkgolide B (GB) and the prodrug of ginkgolide B (PGB) in rat plasma and brain tissue. Detection was performed on a triple quadrupole tandem mass spectrometry in multiple reaction monitoring mode using the electrospray ionization technique in negative ionization mode. The total run time was only 2.0 min. Good linearity was found between 1-200 ng/mL (r > 0.9993) for plasma samples and 0.5-50 ng/mL (r = 0.9995) for the brain tissue samples. The lower limits of quantification of PGB and GB were 1 ng/mL for plasma and 0.5 ng/mL for brain tissue. Intra-day and inter-day precision was less than 11.67%. Intra-day accuracy was in the range of -2.61-10.67%, inter-day accuracy was in the range of -2.36-8.98%. The mean recovery for PGB and GB was between 82.5 and 97.0%. The validated method was successfully applied to a pharmacokinetic study of PGB and GB in rats after intravenous administration.
authors.affiliation: Institute of Natural Medicine, Hefei University of Technology, 193 Tunxi Road, Hefei, PR China. yuanyuan2257@126.com
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Jian
authors.initials: J
authors.lastname: Pan
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Wu
authors.forename: Ailing
authors.initials: A
authors.lastname: Hui
authors.forename: An
authors.initials: A
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D005260:Female; D046934:Ginkgolides / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D007783:Lactones / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D011355:Prodrugs / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D046934:Ginkgolides; D007783:Lactones; D011355:Prodrugs; C045856:ginkgolide B