title: Dissecting the effects of endogenous brain IL-2 and normal versus autoreactive T lymphocytes on microglial responsiveness and T cell trafficking in response to axonal injury.
abstract: IL-2 is essential for T-helper regulatory (Treg) cell function and self-tolerance, and dysregulation of both endogenous brain and peripheral IL-2 gene expression may have important implications for neuronal injury and repair. We used an experimental approach combining mouse congenic breeding and immune reconstitution to test the hypothesis that the response of motoneurons to injury is modulated by the combined effects of IL2-mediated processes in the brain that modulate its endogenous neuroimmunological milieu, and IL2-mediated processes in the peripheral immune system that regulate T cell function (i.e., normal versus autoreactive Treg-deficient T cells). This experimental strategy enabled us to test our hypothesis by disentangling the effect of normal versus autoreactive T lymphocytes from the effect of endogenous brain IL-2 on microglial responsiveness (microglial phagocytic clusters normally associated with dead motoneurons and MHC2(+) activated microglia) and T cell trafficking, using the facial nerve axotomy model of injury. The results demonstrate that the loss of both brain and peripheral IL-2 had an additive effect on numbers of microglial phagocytic clusters at day 14 following injury, whereas the autoreactive status of peripheral T cells was the primary factor that determined the degree to which T cells entered the injured brain and contributed to increased microglial phagocytic clusters. Changes in activated MHC2(+) microglial in the injured FMN were associated with loss of endogenous brain IL-2 and/or peripheral IL-2. This model may provide greater understanding of the mechanisms involved in determining if T cells entering the injured central nervous system (CNS) have damaging or proregenerative effects.
authors.affiliation: Department of Psychiatry, McKnight Brain Institute, University of Florida, Gainesville, FL, USA.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Huang
authors.forename: Danielle
authors.initials: D
authors.lastname: Meola
authors.forename: John M
authors.initials: JM
authors.lastname: Petitto
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015551:Autoimmunity; D001369:Axons / Q000276:immunology / Q000502:physiology*; D019771:Axotomy; D001921:Brain / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement; D004268:DNA-Binding Proteins / Q000235:genetics; D020220:Facial Nerve Injuries / Q000276:immunology / Q000473:pathology; D016867:Immunocompromised Host / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D017628:Microglia / Q000276:immunology / Q000473:pathology / Q000502:physiology*; D009046:Motor Neurons / Q000276:immunology / Q000502:physiology / Q000648:ultrastructure; D015213:Neuroimmunomodulation; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D004268:DNA-Binding Proteins; D007376:Interleukin-2; C499308:Rag2 protein, mouse