title: Differential sensitivity to paclitaxel-induced apoptosis and growth             suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial             adenocarcinoma cells.
abstract: To investigate acquired paclitaxel (PTX) resistance in cancer cells, we             established five monoclonal PTX-resistant cell lines from HEC-1 human endometrial             adenocarcinoma cells by means of long-term PTX-exposed cultures and limiting dilution             cultures. The established PTX-resistant subclones showed apparent resistance to             PTX-induced DNA fragmentation but not to PTX-induced growth suppression. None             of the five PTX-resistant subclones showed apparent resistance to other anticancer             drugs such as cisplatin, etoposide, 5-fluorouracil, pirarubicin-HCl, 4-hydroxy-cyclophosphamide             or mitomycin C. Semiquantitative flow cytometric analysis revealed no apparent             differential expression of 17 molecules that were previously reported to regulate             apoptosis or drug resistance, between the five PTX-resistant subclones and the             parent cells. Karyotyping analysis revealed common changes in chromosomes 4 and             18 in the five PTX-resistant subclones but not in the HEC-1 parent cells. These             results indicate that PTX-induced growth suppression is regulated by different             mechanisms from those involved in PTX-induced apoptosis. It was concluded that             these established PTX-resistant subclones can be useful models in studies related             to the prevention or treatment of recurrent cancers after PTX chemotherapy.
authors.affiliation: Santamaria Hospital, Ibaraki, Osaka 567-0884, Japan. tetanaka@santamaria-med.jp
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Tanaka
authors.forename: Saori
authors.initials: S
authors.lastname: Toujima
authors.forename: Junko
authors.initials: J
authors.lastname: Tanaka
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D019008:Drug Resistance, Neoplasm / Q000235:genetics; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D059785:Karyotype; D017239:Paclitaxel / Q000494:pharmacology*
chemical_list: D000954:Antigens, Surface; D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel