title: [The influence of oxygen partial pressure change and vascularization of rabbit wound through negative pressure wound therapy].
abstract: OBJECTIVE
  To investigate the effect of vacuum sealing drainage (VSD) on variation of oxygen partial pressure (PtO2) and vascularization.
  
  
  METHODS
  The 12 cases of rabbit's wound models were undergoing the VSD (vacuum group, n = 6) or conventional therapy (conventional group, n = 6). Variation of PtO2 was measured by oxygen partial pressure admeasuring apparatus, expression of hypoxia inducible factor 1α (HIF-1α) mRNA was measured by real-time fluorescent quantitative PCR, content of vascular endothelial growth factor (VEGF) was measured by ELISA after tissue homogenate in 7 days. Vascular endothelial cell (VEC) and new blood capillary (NBC) of hematoxylin-eosin slice of tissue were counted by using light microscope.
  
  
  RESULTS
  Average value of PtO2 of vacuum group was significant lower than conventional group (t = -99.780 to -5.305, P < 0.01). Expression of HIF-1α (30 minutes, 1, 6, 12 hours were 3.11 ± 0.07, 3.68 ± 0.26, 4.16 ± 0.13 and 3.91 ± 0.26 respectively) and content of VEGF (30 minutes, 1, 6, 12 hours were 103.3 ± 2.4, 134.2 ± 9.0, 167.8 ± 3.8 and 232.1 ± 9.5 respectively) of vacuum group were increased after 30 minutes and significant lower than conventional group (t = 13.038 - 80.208, P < 0.01), and both of them were reduced after 24 hours (P < 0.05). Counting numbers of VEC (2.47 ± 0.45 to 4.70 ± 0.38) and NBC (1.33 ± 0.49 to 4.33 ± 0.68) of vacuum group were increased at the same time-point and significant higher than conventional group (t = -0.670 to 16.500, P < 0.05).
  
  
  CONCLUSIONS
  PtO2 of wound surface could be reduced significantly by VSD. Expression of HIF-1α and content of VEGF were increased by VSD for enhancing differentiated state of VEC and construction of NBC, which were better for vascularization and wound healing.
authors.affiliation: Department of Traumatic Surgery, the Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Duan
authors.initials: D
authors.lastname: Hu
authors.forename: Xiang-jun
authors.initials: XJ
authors.lastname: Bai
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Ren-jie
authors.initials: RJ
authors.lastname: Li
authors.forename: Chen-chen
authors.initials: CC
authors.lastname: Xue
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008297:Male; D054843:Negative-Pressure Wound Therapy; D018919:Neovascularization, Physiologic; D010100:Oxygen / Q000378:metabolism*; D010313:Partial Pressure; D011817:Rabbits; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014947:Wounds and Injuries / Q000378:metabolism* / Q000473:pathology / Q000628:therapy
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A; D010100:Oxygen