title: [A method for simultaneous assay of propulsion and absorption in small intestine].
abstract: To develop a method for simultaneous assay of propulsion and absorption in small intestine.
  
  
  
  The mice were administrated through gastric tube with mixed reagents containing 0.12% phenol red, D-xylose (1.25%, 2.5% and 5%) and 15% gelatin. The influence of phenol red on D-xylose absorption and the influence of D-xylose on small intestine propulsion rate were investigated by measuring serum concentration of D-xylose with phloroglucinol method.
  
  
  
  At 10 min, no significant difference was found between 5% D-xylose mixed reagent group and 5% D-xylose control. At 15 min, small intestine propulsion rate in 5% D-xylose mixed reagent group, but not in 2.5% and 1.25% D-xylose mixed reagent groups, was significantly higher than in phenol red control (P<0.05).
  
  
  
  Gastric administration of mixed reagent containing 0.12% phenol red, 5% D-xylose and 15% gelatin can simultaneously assay propulsion and absorption of small intestine in mice.
authors.affiliation: School of Medicine and Life Sciences, Zhejiang University City College, Hangzhou 310015, China. liujx@zucc.edu.cn
authors.forename: Jian-xiang
authors.initials: JX
authors.lastname: Liu
authors.forename: Xin-ye
authors.initials: XY
authors.lastname: Zhang
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Pan
authors.forename: Qin-li
authors.initials: QL
authors.lastname: Zhang
authors.forename: Xiang-rong
authors.initials: XR
authors.lastname: Li
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism / Q000502:physiology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010528:Peristalsis; D010637:Phenolsulfonphthalein / Q000493:pharmacokinetics; D014994:Xylose / Q000493:pharmacokinetics
chemical_list: D014994:Xylose; D010637:Phenolsulfonphthalein