title: [Simultaneous determination of 6 forbidden colorants in cosmetics by high performance liquid chromatography-tandem mass spectrometry].
abstract: A method of high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been established for the simultaneous determination of six forbidden colorants including Sudan IV, Acid Violet 49, Sudan Blue 2, Solvent Red 49, Basic Violet 1 and Pigment Orange 5 in cream and powdery matrix cosmetics. The samples were extracted with ethanol-acetonitrile (3:2, v/v) solution by ultrasonic technique for 20 min, then centrifuged for purification and enriched by nitrogen blowing sequentially. The analytes were isolated on a Luna C18 column (150 mm x 2.1 mm, 5 microm) by gradient elution with methanol and 10 mmol/L ammonium acetate as the mobile phases, and detected by MS/MS in the multiple reaction monitoring (MRM) mode. The qualitative analysis was based on the retention time and the relative abundance ratio of the characteristic ions, and the quantitative analysis on calibration curve method. The results showed that the limits of quantification (LOQ, S/N= 10) of the six colorants ranged from 0.1 to 10 microg/kg and the average recoveries were from 86.67% to 98.22% with the relative standard deviations (RSDs) from 4.01% to 7.01%. The method is simple and rapid with high sensitivity and good reproducibility, and suitable for the determination of the six forbidden colorants in cosmetics.
authors.affiliation: Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China. dlciqlwx@sina.com
authors.forename: Weixuan
authors.initials: W
authors.lastname: Lin
authors.forename: Xingquan
authors.initials: X
authors.lastname: Sun
authors.forename: Xuerong
authors.initials: X
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Guiyuan
authors.initials: G
authors.lastname: Guo
mesh_terms: D001391:Azo Compounds / Q000032:analysis; D001557:Benzenesulfonates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis*; D003358:Cosmetics / Q000737:chemistry*; D006801:Humans; D012394:Rosaniline Dyes / Q000032:analysis; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001391:Azo Compounds; D001557:Benzenesulfonates; D004396:Coloring Agents; D003358:Cosmetics; D012394:Rosaniline Dyes; C006394:Acid Violet 6B; C548657:Basic Violet 1; C009213:Scarlet Red