title: [Genetic characteristic of virulent potential of enterococci of human intestinal microbiota].
abstract: OBJECTIVE
  Determine prevalence of genetic determinants of virulence among enterococci strains comprising human intestine microbiota.
  
  
  METHODS
  81 enterococci strains isolated from intestine of individuals during examination for dysbiosis were used in the study. Strain identification was performed by using multiplex PCR. Hemolytic and gelatinase activity was determined by dish method; genes coding virulence factor synthesis (gelE, sprE, cylM, cylB, cylA, esp)--by PCR.
  
  
  RESULTS
  A wide set of genetic determinants of virulence was detected in E. faecalis strain microorganisms.
  
  
  CONCLUSIONS
  Enterococcus genus microorganisms of human intestine microbiota that have virulence potential may become the reason for endogenous infection. The data obtained may be used for prognosis of risk of development of endogenous enterococci infections.
authors.forename: I V
authors.initials: IV
authors.lastname: Valysheva
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D003470:Culture Media; D017931:DNA Primers; D016983:Enterococcus / Q000235:genetics* / Q000302:isolation & purification; D013293:Enterococcus faecalis / Q000235:genetics* / Q000302:isolation & purification; D016984:Enterococcus faecium / Q000235:genetics* / Q000302:isolation & purification; D004912:Erythrocytes / Q000166:cytology; D018093:Gelatinases / Q000032:analysis; D006460:Hemolysin Proteins / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D037521:Virulence Factors / Q000032:analysis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D017931:DNA Primers; D006460:Hemolysin Proteins; D037521:Virulence Factors; D018093:Gelatinases