title: Probing the ArcA regulon in the rumen bacterium Mannheimia succiniciproducens by genome-wide expression profiling.
abstract: In this study, the putative target genes of the Arc two-component system of the rumen bacterium Mannheimia succiniciproducens were determined by analyzing the transcriptome of the ArcA overexpression strain and by the in silico scanning of the entire genome sequence with the position weight matrix of the ArcA binding sequence developed for Escherichia coli. The majority of 79 repressed genes were involved in energy metabolism and carbohydrate transport and metabolism, while the majority of 82 induced genes were involved in hypothetical or unknown functions. Our results suggest that the Arc system in M. succiniciproducens has a specific function that differs from that in E. coli.
authors.affiliation: Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Seulgi
authors.initials: S
authors.lastname: Yun
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Shin
authors.forename: Oh-Cheol
authors.initials: OC
authors.lastname: Kim
authors.forename: Young Ryul
authors.initials: YR
authors.lastname: Jung
authors.forename: Doo-Byoung
authors.initials: DB
authors.lastname: Oh
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.forename: Ohsuk
authors.initials: O
authors.lastname: Kwon
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D019295:Computational Biology / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D018628:Gene Dosage; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D031121:Mannheimia / Q000235:genetics*; D018085:Regulon; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; D015534:Trans-Activators