title: [Model of chronic salmonellosis: parameters of infection and immune response in inbred mice genetically variable in susceptibility to salmonellosis].
abstract: OBJECTIVE
  Study parameters of chronic infection and immune response in I/St and A/Sn line mice in the model of per oral infection of mice with Salmonella enterica serovar Typhimurium.
  
  
  METHODS
  Studies were carried out in I/StSnEgYCit (I/St), A/JsnYCit (A/Sn) inbred line mice as well as their back crossing hybrids [I/StrxF1(I/StxA/Sn)]BC. Mice were infected per os by S. enterica serovar Typhimurium strain IE147 at a dose of 2 x 10(5) PFU per mice. The number of salmonellae was determined at days 3, 5 and 7, weeks 3 and 4 after the infection in various organs, the number of antibody producers--by cell EIA. Pathomorphologic changes in mice spleens were studied histologically by using hematoxylin and eosin staining. In offspring of back crossing [I/St x F1(I/St x A/Sn)]BCl segregation genetic analysis of sensitivity to salmonella infection trait and mapping of loci taking part in salmonella infection were carried out.
  
  
  RESULTS
  The course of chronic salmonellosis in susceptible I/St line was characterized by the presence of more pronounced pathomorphologic changes in spleen and significantly higher microbial load in organs (approximately by 1000 times) when compared with A/Sn mice. Interlinear differences in susceptibility to infection correlated with differences in the type of early local and systemic immune response. In I/St mice a higher level of salmonella specific IgG2a-, IgG1- and IgA forming cells in spleen compared with A/Sn mice was detected which correlates with a pronounced splenomegaly and high concentration of salmonellae. On the contrary A/Sn mice demonstrated a higher level of salmonella specific IgA forming cells in Peyer patches that probably leads to protection of A/Sn line during per oral infection. Genetic analysis of susceptibility to salmonellosis trait inheritance showed the presence of its coupling with D9Mit89 locus of chromosome 9 on which previously Tbs2 locus was mapped that plays a role in the control of tuberculosis infection.
  
  
  CONCLUSIONS
  There is a probability of the presence of general mechanisms of genetic control of tuberculosis and salmonella infections in A/Sn and I/St mice.
authors.forename: L N
authors.initials: LN
authors.lastname: Nesterenko
authors.forename: N V
authors.initials: NV
authors.lastname: Kobets
authors.forename: D V
authors.initials: DV
authors.lastname: Balunets
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D002908:Chronic Disease; D015169:Colony Count, Microbial; D003433:Crosses, Genetic; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004801:Eosine Yellowish-(YS); D056426:Genetic Loci / Q000276:immunology*; D020022:Genetic Predisposition to Disease; D006416:Hematoxylin; D058507:Host Specificity; D007132:Immunoglobulin Isotypes / Q000097:blood / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009169:Mycobacterium tuberculosis / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D012486:Salmonella typhimurium / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D014376:Tuberculosis / Q000235:genetics / Q000276:immunology / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D007132:Immunoglobulin Isotypes; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin