title: Collagen type I together with fibronectin provide a better support for endothelialization.
abstract: Endothelialization of vascular implants is limited by the inability of cells to retain adhesion when exposed to flow. Extracellular matrix proteins, including fibronectin and collagen, enhance cell adherence on materials. This study investigated the behaviour of Human Umbilical Vein Endothelial Cells (HUVEC) on extracellular matrix coated polystyrene. Collagen and fibronectin were coated as single and double layers to analyse differences in cell proliferation, morphology, and cell-protein interactions. Significantly higher endothelial cell proliferation and migration rates were observed on the collagen and collagen+fibronectin coating compared to the uncoated or fibronectin-coated sample. Immmunofluorescent microscopy showed evidence of extracellular matrix remodelling in the double, collagen+fibronectin coating. These results strongly suggest that a double coating of collagen+fibronectin provides a better support structure for endothelial cell growth and contributes to improve the ability of vascular implants to become and remain endothelialized.
authors.affiliation: Université de technologie de Compiègne, UMR CNRS 7338, biomécanique et bio-ingénierie, centre de recherches de Royallieu, BP 20529, 60205 Compiègne cedex, France.
authors.forename: Melissa
authors.initials: M
authors.lastname: Sgarioto
authors.forename: Pascale
authors.initials: P
authors.lastname: Vigneron
authors.forename: John
authors.initials: J
authors.lastname: Patterson
authors.forename: François
authors.initials: F
authors.lastname: Malherbe
authors.forename: Marie-Danielle
authors.initials: MD
authors.lastname: Nagel
authors.forename: Christophe
authors.initials: C
authors.lastname: Egles
mesh_terms: D000327:Adsorption; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D024042:Collagen Type I / Q000494:pharmacology*; D042783:Endothelial Cells / Q000502:physiology / Q000648:ultrastructure; D004730:Endothelium, Vascular / Q000187:drug effects / Q000254:growth & development*; D005109:Extracellular Matrix / Q000187:drug effects / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D011137:Polystyrenes; D011554:Pseudopodia / Q000502:physiology
chemical_list: D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D011137:Polystyrenes