title: Bone marrow-derived HipOP cell population is markedly enriched in osteoprogenitors.
abstract: We recently succeeded in purifying a novel multipotential progenitor or stem cell population from bone marrow stromal cells (BMSCs). This population exhibited a very high frequency of colony forming units-osteoblast (CFU-O; 100 times higher than in BMSCs) and high expression levels of osteoblast differentiation markers. Furthermore, large masses of mineralized tissue were observed in in vivo transplants with this new population, designated highly purified osteoprogenitors (HipOPs). We now report the detailed presence and localization of HipOPs and recipient cells in transplants, and demonstrate that there is a strong relationship between the mineralized tissue volume formed and the transplanted number of HipOPs.
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamada-oka Suita, Osaka 565-0871, Japan.|Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Room 4245, Medical Sciences Building, 1 King's College Circle, Toronto, ON M5S 1A8, Canada.
authors.forename: Shousaku
authors.initials: S
authors.lastname: Itoh
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamada-oka Suita, Osaka 565-0871, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Matsushita
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamada-oka Suita, Osaka 565-0871, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Ikeda
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamada-oka Suita, Osaka 565-0871, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamada-oka Suita, Osaka 565-0871, Japan.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Yamauchi
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamada-oka Suita, Osaka 565-0871, Japan.
authors.forename: Seisuke
authors.initials: S
authors.lastname: Yoshioka
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamada-oka Suita, Osaka 565-0871, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Yamamoto
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamada-oka Suita, Osaka 565-0871, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Ebisu
authors.affiliation: Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8, Yamada-oka Suita, Osaka 565-0871, Japan.
authors.forename: Mikako
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Room 4245, Medical Sciences Building, 1 King's College Circle, Toronto, ON M5S 1A8, Canada.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Aubin
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D002454:Cell Differentiation; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D008822:Mice, Transgenic; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology*