title: 3,4,5-tricaffeoylquinic Acid inhibits the lipopolysaccharide-stimulated production of inflammatory mediators in keratinocytes.
abstract: OBJECTIVE
  Microbial product lipopolysaccharide (LPS) has been shown to be involved in the pathogenesis of inflammatory skin diseases. Caffeoyl derivatives have demonstrated anti-inflammatory and antioxidant effects. However, the effect of 3,4,5-tricaffeoylquinic acid (3,4,5-triCQA) on the production of microbial product-induced inflammatory mediators in keratinocytes has not yet been studied.
  
  
  METHODS
  Using human keratinocytes, we investigated the effect of 3,4,5-triCQA on the LPS-stimulated production of inflammatory mediators in relation to the nuclear factor (NF)-ĸB, Akt and ERK pathways.
  
  
  RESULTS
  3,4,5-triCQA inhibited the LPS-induced expression of Toll-like receptor 4, and the production of cytokines and chemokines in keratinocytes. 3,4,5-triCQA, Bay 11-7085, Aĸt inhibitor and ERK inhibitor each attenuated the LPS-induced production of inflammatory mediators by inhibiting the NF-ĸB, Akt and ERK pathways.
  
  
  CONCLUSIONS
  3,4,5-triCQA may attenuate the LPS-stimulated production of inflammatory mediators in keratinocytes by suppressing the Toll-like receptor 4 expression-mediated activation of the Akt, ERK and NF-ĸB pathways. 3,4,5-triCQA may exert a preventive effect against microbial product-induced inflammatory skin diseases.
authors.affiliation: Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, South Korea.
authors.forename: Seon Ae
authors.initials: SA
authors.lastname: Lee
authors.forename: Eun Byul
authors.initials: EB
authors.lastname: Jung
authors.forename: Seon Hwa
authors.initials: SH
authors.lastname: Lee
authors.forename: Yun Jeong
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hyoweon
authors.initials: H
authors.lastname: Bang
authors.forename: Seong Jun
authors.initials: SJ
authors.lastname: Seo
authors.forename: Young Wook
authors.initials: YW
authors.lastname: Choi
authors.forename: Manh Heun
authors.initials: MH
authors.lastname: Kim
authors.forename: Min Won
authors.initials: MW
authors.lastname: Lee
authors.forename: Chung Soo
authors.initials: CS
authors.lastname: Lee
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors*; D016328:NF-kappa B / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011801:Quinic Acid / Q000031:analogs & derivatives
chemical_list: D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; C472707:caffeoylquinic acid; D011801:Quinic Acid; D002726:Chlorogenic Acid; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases