title: Monitoring conformational dynamics with single-molecule fluorescence energy transfer: applications in nucleosome remodeling.
abstract: Due to its ability to track distance changes within individual molecules or molecular complexes on the nanometer scale and in real time, single-molecule fluorescence resonance energy transfer (single-molecule FRET) is a powerful tool to tackle a wide range of important biological questions. Using our recently developed single-molecule FRET assay to monitor nucleosome translocation as an illustrative example, we describe here in detail how to set up, carry out, and analyze single-molecule FRET experiments that provide time-dependent information on biomolecular processes.
authors.affiliation: Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Deindl
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhuang
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D002021:Buffers; D042002:Chromatin Assembly and Disassembly; D004247:DNA / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D002021:Buffers; D005456:Fluorescent Dyes; D006657:Histones; C086468:ISWI protein; D009707:Nucleosomes; D014157:Transcription Factors; D011092:Polyethylene Glycols; D004247:DNA; D000251:Adenosine Triphosphatases