title: [Morphofunctional changes of BALB/c and C57BL/6 mice's immune system under chronic bacterial gram-negative endotoxicosis].
abstract: Chronic endotoxicosis was modeled by subcutaneous injection of the sepharose in complex with LPS. In these conditions we have studied morphofunctional changes of the immune system of BALB/c and C57Bl/6 mice, which are characterized by the different types of the immune response (Th2 type is predominant in BALB/c, Th1--in C57Bl/6). In the 1st-7th day t in the serum of BALB/c mice the endotoxin level increased in 21.3 times, in C57Bl/6--in 20.6 times. The endotoxin antibodies significantly decreased in 1th-7th days, on the 14th day it increased in the serum of both mice's strains. Morphofunctional changes of the immune system after chronic endotoxicosis were different in BALB/c and C57BI/6 mice. On the 1th day after injection of LPS and sepharose, in the thymus of C57Bl/6 mice the cortex layer was exhausted because of cell death, in the thymus of BALB/c mice II-III stages of accidental involution were developed. On the 7-14th day after injection of LPS and sepharose in the spleen of C57Bl/6 mice T- and B-zones were hyperplastic, however in spleen of BALB/c mice only T-zone were enlarged. After LPS and sepharose injection changes of cytokine production synthesized by KonA activated splenic cells were found out. In both strains the level of proinflammatory cytokines--TNFalpha and IL-1beta decreased, as well the Th1-cytokine IL-2. The production o fTh2-cytokine - IL-4, significantly decreased only in C57BI/6 mice. We suggest that damaging effect of LPS injection is determined by predominant Th2 or Th2 types of the immune response.
authors.forename: O V
authors.initials: OV
authors.lastname: Makarova
authors.forename: M E
authors.initials: ME
authors.lastname: Diatroptov
authors.forename: S N
authors.initials: SN
authors.lastname: Serebriakov
authors.forename: V V
authors.initials: VV
authors.lastname: Malaĭtsev
authors.forename: I M
authors.initials: IM
authors.lastname: Bogdanova
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood; D016207:Cytokines / Q000187:drug effects / Q000378:metabolism; D004731:Endotoxins / Q000008:administration & dosage*; D006090:Gram-Negative Bacteria / Q000472:pathogenicity; D007111:Immunity, Cellular / Q000187:drug effects* / Q000235:genetics*; D007279:Injections, Subcutaneous; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics / Q000276:immunology; D008810:Mice, Inbred C57BL / Q000235:genetics / Q000276:immunology; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D018417:Th1 Cells / Q000187:drug effects / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology / Q000473:pathology
chemical_list: D000906:Antibodies; D016207:Cytokines; D004731:Endotoxins; D008070:Lipopolysaccharides