title: New approaches for bottom-up assembly of tobacco mosaic virus-derived nucleoprotein tubes on defined patterns on silica- and polymer-based substrates.
abstract: The capability of some natural molecular building blocks to self-organize into defined supramolecular architectures is a versatile tool for nanotechnological applications. Their site-selective integration into a technical context, however, still poses a major challenge. RNA-directed self-assembly of tobacco mosaic virus-derived coat protein on immobilized RNA scaffolds presents a possibility to grow nucleoprotein nanotubes in place. Two new methods for their site-selective, bottom-up assembly are introduced. For this purpose, isothiocyanate alkoxysilane was used to activate oxidic surfaces for the covalent immobilization of DNA oligomers, which served as linkers for assembly-directing RNA. Patterned silanization of surfaces was achieved (1) on oxidic surfaces via dip-pen nanolithography and (2) on polymer surfaces (poly(dimethylsiloxane)) via selective oxidization by UV-light irradiation in air. Atomic force microscopy and X-ray photoelectron spectroscopy were used to characterize the surfaces. It is shown for the first time that the combination of the mentioned structuring methods and the isothiocyanate-based chemistry is appropriate (1) for the site-selective immobilization of nucleic acids and, thus, (2) for the formation of viral nanoparticles by bottom-up self-assembly after adding the corresponding coat proteins.
authors.affiliation: Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
authors.forename: Carlos
authors.initials: C
authors.lastname: Azucena
authors.forename: Fabian J
authors.initials: FJ
authors.lastname: Eber
authors.forename: Vanessa
authors.initials: V
authors.lastname: Trouillet
authors.forename: Michael
authors.initials: M
authors.lastname: Hirtz
authors.forename: Stefan
authors.initials: S
authors.lastname: Heissler
authors.forename: Matthias
authors.initials: M
authors.lastname: Franzreb
authors.forename: Harald
authors.initials: H
authors.lastname: Fuchs
authors.forename: Christina
authors.initials: C
authors.lastname: Wege
authors.forename: Hartmut
authors.initials: H
authors.lastname: Gliemann
mesh_terms: D004247:DNA / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry*; D009698:Nucleoproteins / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D014027:Tobacco Mosaic Virus / Q000737:chemistry*
chemical_list: D004129:Dimethylpolysiloxanes; D009698:Nucleoproteins; C013830:baysilon; D012313:RNA; D012822:Silicon Dioxide; D004247:DNA