title: Embedded soft-tissue image mechanism of a small animal shell with synchrotron-based micro-CT.
abstract: Synchrotron-based micro-CT was utilized to image the embedded biological soft-tissue of a small animal shell. Micro-CT images of the biological soft-tissue were acquired using 20, 25, and 27 keV synchrotron X-rays with contrast agents, such as water, physiological saline and iodine. Visualized the complex features of the animal at the above energies with water, physiological saline and iodine. The choice of the optimum energy was chosen based on the contrast mechanisms to know more about soft-matter and the associated internal complex biological features of the small animal shell. This way, the images at 27 keV (optimum energy) was reasonably acceptable for better visualization of the interior micro-architecture, such as soft-anatomy, physiology and internal organs of the animal with better visibility. The introduction of water, physiological, or iodine as contrast agents is shown to enhance minute image features in synchrotron-based tomographic imaging.
authors.affiliation: Istituto di Matematica e Fisica, Università degli Studi di Sassari, Italy. dvrao@uniss.it
authors.forename: Donepudi V
authors.initials: DV
authors.lastname: Rao
authors.forename: Roberto
authors.initials: R
authors.lastname: Cesareo
authors.forename: Antonio
authors.initials: A
authors.lastname: Brunetti
authors.forename: Tako
authors.initials: T
authors.lastname: Akatsuka
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yuasa
authors.forename: Tohoru
authors.initials: T
authors.lastname: Takeda
authors.forename: Giovanni E
authors.initials: GE
authors.lastname: Gigante
mesh_terms: D060105:Animal Shells / Q000000981:diagnostic imaging*; D000818:Animals; D003287:Contrast Media / Q000737:chemistry; D021621:Imaging, Three-Dimensional; D007455:Iodine / Q000737:chemistry; D012908:Snails / Q000033:anatomy & histology*; D012965:Sodium Chloride / Q000737:chemistry; D017356:Synchrotrons; D014867:Water / Q000737:chemistry; D055114:X-Ray Microtomography / Q000295:instrumentation* / Q000379:methods*
chemical_list: D003287:Contrast Media; D014867:Water; D012965:Sodium Chloride; D007455:Iodine