title: Bcl-2 associated anthanogen-1 (Bag-1) expression and prognostic value in pancreatic head and periampullary cancer.
abstract: The expression of anti-apoptosis gene Bcl-2 associated anthanogen-1 (Bag-1), has been associated with outcome in several cancer types, however its prognostic role in pancreatic cancer is unknown. Aim was therefore to evaluate expression of Bag-1 in two anatomically closely related however prognostically different tumours, pancreatic head- and periampullary cancer and correlate expression with outcome. Bag-1 protein expression was studied by immunohistochemistry on original paraffin embedded tissue from 217 patients with microscopic radical resection (R0) of adenocarcinoma of the pancreatic head or periampullary region. Expression was assessed for associations with recurrence free- (RFS), cancer specific- (CSS), overall survival (OS) and conventional prognostic factors. Nuclear Bag-1 was present in 80% of tumours. In 40% Bag-1 resided in the cytosol, which was almost exclusively associated with nuclear expression. Nuclear Bag-1 protein was identified as an independent factor predicting a favourable outcome following radical resection of pancreatic head cancer. Eighteen percent of patients with nuclear Bag-1 were recurrence free and alive 5 years following surgery compared to none of the patients lacking expression. In periampullary cancer Bag-1 was not associated with outcome. In conclusion, Bag-1 was present in the majority of both pancreatic head- and periampullary cancers. However it was only identified as a discriminator of outcome in pancreatic head cancer.
authors.affiliation: Department of Surgery, Erasmus Medical Center, Rotterdam, The Netherlands. j.a.vanderzee@erasmusmc.nl
authors.forename: Jill A
authors.initials: JA
authors.lastname: van der Zee
authors.forename: Timo L M
authors.initials: TL
authors.lastname: Ten Hagen
authors.forename: Wim C J
authors.initials: WC
authors.lastname: Hop
authors.forename: Herman
authors.initials: H
authors.lastname: van Dekken
authors.forename: Bilyana M
authors.initials: BM
authors.lastname: Dicheva
authors.forename: Ann L B
authors.initials: AL
authors.lastname: Seynhaeve
authors.forename: Gerben A
authors.initials: GA
authors.lastname: Koning
authors.forename: Alexander M M
authors.initials: AM
authors.lastname: Eggermont
authors.forename: Casper H J
authors.initials: CH
authors.lastname: van Eijck
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014670:Ampulla of Vater / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016612:Paraffin Embedding; D011379:Prognosis; D016032:Randomized Controlled Trials as Topic; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D016896:Treatment Outcome
chemical_list: C091732:BCL2-associated athanogene 1 protein; D004268:DNA-Binding Proteins; D014157:Transcription Factors