title: Oncogenic miR-544 is an important molecular target in gastric cancer.
abstract: MicroRNAs (miRNAs) and promoter hypermethylation are vital epigenetic mechanisms for transcriptional inactivation of tumor suppressor. IRX1 is a newly identified tumor suppressor gene and hypermethylation involves the decreased expression in gastric cancer. However, the microRNA regulatory mechanism on IRX1 expression is still unclear. In this study, we report an IRX1-targeting miRNA-544, which directly targets 3'-UTR of IRX1 gene by luciferase reporter assay. miR-544 suppresses the protein expression of IRX1 gene by Western blot and immunocytochemistry. Ectopic expression of miR-544 promotes cell proliferation and cell cycle progression significantly in vitro on gastric cancer cells. The study suggests that miR-544 is an oncogenic microRNA in gastric cancer. Over expression of miR-544 contributes to the inactivation and low-expression of IRX1 in gastric cancer. These findings are helpful for clarifying the molecular mechanisms involved in gastric carcinogenesis and indicate that miR-544 is a key regulator in switching cell cycle on or off. miR-544 may be a potential molecular target in miRNA-based strategy on gastric cancer.
authors.affiliation: Department of Surgery of Ruijin Hospital, Shanghai Key Laboratory for Gastric Neoplasia, Affiliated to Shanghai Jiao Tong University, School of Medicine, Ruijin er Road, No. 197, 200025, Shanghai, China.
authors.forename: Qiaoming
authors.initials: Q
authors.lastname: Zhi
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Guo
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Rongjuan
authors.initials: R
authors.lastname: Zhang
authors.forename: Jinling
authors.initials: J
authors.lastname: Jiang
authors.forename: Jun
authors.initials: J
authors.lastname: Ji
authors.forename: Jianian
authors.initials: J
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Xuehua
authors.initials: X
authors.lastname: Chen
authors.forename: Qu
authors.initials: Q
authors.lastname: Cai
authors.forename: Jianfang
authors.initials: J
authors.lastname: Li
authors.forename: Bingya
authors.initials: B
authors.lastname: Liu
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Yu
mesh_terms: D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D058990:Molecular Targeted Therapy; D012334:RNA, Neoplasm / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C582640:MIRN544 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm