title: Proteomic analysis for detecting serum biomarkers related to smoking in humans.
abstract: BACKGROUND
  Smoking is the leading cause of death in the world. This study focused on the difference of the serum proteomic profiling between healthy smokers and nonsmokers in order to find smoking-specific serum biomarkers.
  
  
  METHODS
  Pattern-based proteomic profiling of 100 serum samples (from 50 Chinese male smokers and 50 matched nonsmokers) was performed through magnetic bead fractionation coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis (MALDI-TOF-MS) and resulting data were statistically analyzed by Ciphergen ProteinChip software 3.0.2.
  
  
  RESULTS
  We found 72 serum peaks were significantly different between smokers and nonsmokers (P < 0.05). Marker peaks of mass-to-charge ratio (m/z) 3159.13, 7561.03 and 9407.32 were smoking-specific.
  
  
  CONCLUSIONS
  The preliminary data suggested that smoking-specific serum biomarkers could be detected in humans.
authors.affiliation: Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders, Beijing Institute of Respiratory Medicine, WHO Collaborating Center for Tobacco or Health, Beijing 100020, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xiao
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhao
authors.forename: Shui-Lian
authors.initials: SL
authors.lastname: Chu
authors.forename: Hang
authors.initials: H
authors.lastname: Jing
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D040901:Proteomics / Q000379:methods*; D012907:Smoking / Q000097:blood*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D001798:Blood Proteins