title: Study on the conformation changes of Lysozyme induced by Hypocrellin A: the mechanism investigation.
abstract: The interactions between Lysozyme and Hypocrellin A are investigated in details using time-resolved fluorescence, fourier transform infrared spectroscopy (FTIR), circular dichroism spectroscopy (CD), three-dimensional fluorescence spectra, and thermal gravimetric analysis (TGA) techniques. The results of time-resolved fluorescence suggest that the quenching mechanism is static quenching. FTIR and CD spectroscopy provide evidences of the reducing of α-helix after interaction. Hypocrellin A could change the micro-environmental of Lysozyme according to hydrophobic interaction between the aromatic ring and the hydrophobic amino acid residues, and the altered polypeptide backbone structures induce the reduction of α-helical structures. Moreover, TGA study further demonstrates the structure changes of Lysozyme on the effect of Hypocrellin A. This study could provide some important information for the derivatives of HA in pharmacy, pharmacology and biochemistry.
authors.affiliation: Analysis and Testing Center, Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Key Laboratory of Environmental Change and Ecological Construction, School of Geograph Science, Nanjing Normal University, Nanjing 210046, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
authors.forename: He-Yong
authors.initials: HY
authors.lastname: Huang
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.forename: Jia-Hong
authors.initials: JH
authors.lastname: Zhou
authors.forename: Zheng-Ming
authors.initials: ZM
authors.lastname: Liu
mesh_terms: D000818:Animals; D002645:Chickens; D002942:Circular Dichroism; D004735:Energy Transfer; D009113:Muramidase / Q000737:chemistry*; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D019800:Phenol; D017433:Protein Structure, Secondary; D011809:Quinones / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D013997:Time Factors
chemical_list: D011809:Quinones; D012996:Solutions; D019800:Phenol; D010569:Perylene; D009113:Muramidase; C048388:hypocrellin A