title: Segmenting data sets for RIP.
abstract: Specific radiation damage can be used for the phasing of macromolecular crystal structures. In practice, however, the optimization of the X-ray dose used to `burn' the crystal to induce specific damage can be difficult. Here, a method is presented in which a single large data set that has not been optimized in any way for radiation-damage-induced phasing (RIP) is segmented into multiple sub-data sets, which can then be used for RIP. The efficacy of this method is demonstrated using two model systems and two test systems. A method to improve the success of this type of phasing experiment by varying the composition of the two sub-data sets with respect to their separation by image number, and hence by absorbed dose, as well as their individual completeness is illustrated.
authors.affiliation: Structural Biology Group, European Synchrotron Radiation Facility, Grenoble, France.
authors.forename: Daniele
authors.initials: D
authors.lastname: de Sanctis
authors.forename: Max H
authors.initials: MH
authors.lastname: Nanao
mesh_terms: D000818:Animals; D002417:Cattle; D018360:Crystallography, X-Ray / Q000379:methods*; D032426:Marantaceae / Q000737:chemistry; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
chemical_list: D011506:Proteins