title: Immobilization of pectinase and lipase on macroporous resin coated with chitosan for treatment of whitewater from papermaking.
abstract: Anionic residues and pitch deposits in whitewater negatively impact the operation of paper-forming equipment. In order to remove these substances, a macroporous resin based on a methyl acrylate matrix was synthesized and coated with chitosan of various molecular weights through glutaraldehyde cross-linking. Pectinase from Bacillus licheniformis and lipase from Thermomyces lanuginosus were immobilized on the resin coated with chitosan by a Schiff base reaction. The highest hydrolysis activities of the immobilized enzymes were achieved by using chitosan with 10×10(5)DaMW for coating and 0.0025% glutaraldehyde for cross-linking chitosan. The cationic demand and pitch deposits in whitewater were reduced by 58% and 74%, respectively, when treating whitewater with immobilized dual-enzymes for 15min at 55°C and pH 7.5. This method is useful for treatment of whitewater in the papermaking industry.
authors.affiliation: College of Materials Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, China. liuk1103@yahoo.com.cn
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.forename: Guanglei
authors.initials: G
authors.lastname: Zhao
authors.forename: Beihai
authors.initials: B
authors.lastname: He
authors.forename: Lihui
authors.initials: L
authors.lastname: Chen
authors.forename: Liulian
authors.initials: L
authors.lastname: Huang
mesh_terms: D001203:Ascomycota / Q000201:enzymology; D001407:Bacillus / Q000201:enzymology; D048271:Chitosan / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005976:Glutaral / Q000494:pharmacology; D008049:Lipase / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D010209:Paper; D010316:Particle Size; D011096:Polygalacturonase / Q000378:metabolism*; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D062065:Wastewater / Q000737:chemistry*; D018508:Water Purification / Q000379:methods*
chemical_list: D048271:Chitosan; D004800:Enzymes, Immobilized; D005976:Glutaral; D008049:Lipase; D011096:Polygalacturonase; D012117:Resins, Synthetic; D062065:Wastewater