title: Chicken IL-17F: identification and comparative expression analysis in Eimeria-infected chickens.
abstract: Interleukin-17F (IL-17F) is a proinflammatory cytokine, which plays an important role in gut homeostasis. A full-length chicken IL-17F (chIL-17F) cDNA with a 510-bp coding region was identified from ConA-activated chicken splenic lymphocytes. ChIL-17F shares 53% amino acid sequence identity with the previously described chicken IL-17 (chIL-17A) and 38-43% with mammalian homologues. The locus harboring chIL-17 and chIL-17F displayed inverted order compared to those of mammals. ChIL-17F transcript expression was high in lymphoblast cell line CU205 and at moderate levels in small and large intestines and liver. ChIL-17F and chIL-17 expression profiles were examined by quantitative real-time RT-PCR in mitogen-stimulated splenic lymphocytes and intestinal areas affected by Eimeria maxima and Eimeria tenella infections. Expression levels of chIL-17F, like chIL-17, were elevated in mitogen-activated splenic lymphocytes. ChIL-17F, but not chIL-17, expression was upregulated in intestinal tissues affected by E. maxima and E. tenella infections. Recombinant chIL-17F biological activities were similar to that of chIL-17 in primary chicken embryonic fibroblasts. These results suggest that chIL-17F is a unique member of the IL-17 family of cytokines.
authors.affiliation: College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Woo H
authors.initials: WH
authors.lastname: Kim
authors.forename: Jipseol
authors.initials: J
authors.lastname: Jeong
authors.forename: Ae R
authors.initials: AR
authors.lastname: Park
authors.forename: Dongjean
authors.initials: D
authors.lastname: Yim
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Kim
authors.forename: Kwang D
authors.initials: KD
authors.lastname: Kim
authors.forename: Hong H
authors.initials: HH
authors.lastname: Chang
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: Byung-Hyung
authors.initials: BH
authors.lastname: Lee
authors.forename: Wongi
authors.initials: W
authors.lastname: Min
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology*; D004539:Eimeria / Q000276:immunology*; D020381:Interleukin-17 / Q000032:analysis* / Q000235:genetics*; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D020381:Interleukin-17; D008934:Mitogens; D012333:RNA, Messenger