title: Radiation-responsive transcriptome analysis in human lymphoid cells.
abstract: Ionising radiation (IR) causes DNA (deoxyribonucleic acid) injury and activates intracellular signal pathways including the regulation of DNA repair and cell cycle. However, the further knowledge of molecular events involved in radiation exposure is essential to more comprehensively understand the effects of irradiation. Therefore, the gene expressions of mRNA (messenger ribonucleic acid) by X-ray irradiation in human B lymphoblast cell line (IM-9) using a microarray were investigated. The mRNA expressions of 65 genes were shown to be up-regulated at >2.0-fold in irradiated cells (4 Gy) when compared with non-irradiated cells (0 Gy) by microarray analysis. Among 65 genes, a large number of genes were up-regulated with an X-ray dose-dependent change. These results indicate that the up-regulation of their mRNAs is the effects of irradiation and may be due to biological dosimetric markers for the evaluation of radiation exposure in the future.
authors.affiliation: Division of Medical Life Sciences, Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori 036-8564, Japan. mchiba32@cc.hirosaki-u.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Chiba
mesh_terms: D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D020869:Gene Expression Profiling / Q000379:methods*; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011839:Radiation, Ionizing; D011874:Radiometry / Q000379:methods; D015854:Up-Regulation; D014965:X-Rays
chemical_list: D012333:RNA, Messenger; D012313:RNA