title: Genotoxic effects of tacrolimus on human lymphocyte cells.
abstract: We designed in vitro study to determine possible genotoxic effects oftacrolimus (FK-506), which is used as a potent immunosuppressive drug, by using sister chromatid exchange (SCEs), chromosome aberration (CAs), micronuclei tests (MN) and cell growth kinetics such as mitotic index (MI) and replication index (RI) in human lymphocytes. The cells were treated with 5, 25, 50, and 100 ng/mL concentrations of tacrolimus, for 24 h and 48 h treatment periods. Tacrolimus induced CA and MN frequency at all concentrations for 24 and 48 h In additon, it induced the SCE at the highest concantration for 24 h and at 25 and 100 ng/mL for 48 h. Tacrolimus decreased MI at all concentrations (except 5 ng/mL) for all treatment periods. It also inhibited the RI at 50 and 100 ng/mL concentrations for 24 h and at all concentrations for 48 h. Treatments given with tacrolimus result in the enhance of the different endpoints ofgenotoxicity, suggesting its mutagenic action on lymphocytes in vitro.
authors.affiliation: Department of Medical Biology and Genetics Inonu University, Medical Faculty, Malatya 44280, Turkey.
authors.forename: E L
authors.initials: EL
authors.lastname: Kurtoglu
authors.forename: S
authors.initials: S
authors.lastname: Yuksel
mesh_terms: D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects*; D015162:Micronucleus Tests; D008940:Mitotic Index; D012854:Sister Chromatid Exchange / Q000187:drug effects; D016559:Tacrolimus / Q000494:pharmacology*
chemical_list: D007166:Immunosuppressive Agents; D016559:Tacrolimus