title: Application of biomarkers in mudskipper (Boleophthalmus dussumieri) to assess polycyclic aromatic hydrocarbons (PAHs) pollution in coastal areas of the Persian Gulf.
abstract: In this study, selected biomarkers-The retention time (RT) performed on haemocytes, the Erythrocyte Osmotic Fragility (EOF) and the Glutathione-S Transferase (GST) activity in the liver- were measured in mudskipper (i.e., Boleophthalmus dussumieri) obtained from the Persian Gulf. Chemical analyses included the assessment of the polycyclic aromatic hydrocarbons (PAHs) in the sediment and mudskipper liver. Total PAH concentrations in the sediment and the liver tissues ranged between 113.50-3384.34 ng g(-1) dw and 3.99-46.64 ng g(-1) dw, respectively. Spatial evaluation indicated that RT had a significant difference (p<0.05) among sampling sites except Arvand and Zangi. The mean erythrocyte fragility was significantly differ between various locations (p<0.05). GST activity in the liver of mudskippers showed significant differences (p<0.05) among Jafari, Bahrakan, and with other sites. No significant difference (p>0.05) was observed between Arvand, Zangi and Samayeli. Additionally, significant correlations were found between total PAHs levels and selected biomarkers (p<0.01). The biomarkers measured in this study were useful as a first investigation into the biological effects of PAHs pollution as well as in determining the bioavailability of pollution.
authors.affiliation: Department of marine biology, Graduate school of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran. oceanography.sina@gmail.com
authors.forename: Mahmood
authors.initials: M
authors.lastname: Sinaei
authors.forename: Peyman Eghtesadi
authors.initials: PE
authors.lastname: araghi
authors.forename: Ali
authors.initials: A
authors.lastname: Mashinchian
authors.forename: Mohamadreza
authors.initials: M
authors.lastname: Fatemi
authors.forename: Gholamhossein
authors.initials: G
authors.lastname: Riazi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D004784:Environmental Monitoring; D004789:Enzyme Activation / Q000187:drug effects; D005982:Glutathione Transferase / Q000378:metabolism; D006434:Hemocytes / Q000187:drug effects; D019156:Indian Ocean; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D010473:Perciformes / Q000378:metabolism / Q000502:physiology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D015415:Biomarkers; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D005982:Glutathione Transferase