title: Quinolone-resistant Shigella flexneri isolated in a patient who travelled to India.
abstract: We report a recent case in which ciprofloxacin-resistant Shigella flexneri was isolated from a 23-yr-old female patient with a history of travel to India. Prior to her admission to our internal medicine department, she experienced symptoms of high fever and generalized weakness from continuous watery diarrhea that developed midway during the trip. S. flexneri was isolated from the stool culture. Despite initial treatment with ciprofloxacin, the stool cultures continued to show S. flexneri growth. In the susceptibility test for antibiotics of the quinolone family, the isolate showed resistance to ciprofloxacin (minimum inhibitory concentration [MIC], 8 µg/mL), norfloxacin (MIC, 32 µg/mL), ofloxacin (MIC, 8 µg/mL), nalidixic acid (MIC, 256 µg/mL), and intermediate resistance to levofloxacin (MIC, 4 µg/mL). In molecular studies for quinolone resistance related genes, plasmid borne-quinolone resistance genes such as qnrA, qnrB, qnrS, aac(6')-Ib-cr, qepA, and oqxAB were not detected. Two mutations were observed in gyrA (248C→T, 259G→A) and 1 mutation in parC (239G→T). The molecular characteristics of the isolated S. flexneri showed that the isolate was more similar to the strains isolated from the dysentery outbreak in India than those isolated from Korea.
authors.affiliation: Department of Medicine, Graduate School, Kyung Hee University, Seoul, Korea.
authors.forename: You La
authors.initials: YL
authors.lastname: Jeon
authors.forename: You-sun
authors.initials: YS
authors.lastname: Nam
authors.forename: Gayoung
authors.initials: G
authors.lastname: Lim
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Cho
authors.forename: Yun-Tae
authors.initials: YT
authors.lastname: Kim
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Jang
authors.forename: Junyoung
authors.initials: J
authors.lastname: Kim
authors.forename: Misun
authors.initials: M
authors.lastname: Park
authors.forename: Hee Joo
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D004405:Dysentery, Bacillary / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007194:India; D009154:Mutation; D015363:Quinolones / Q000494:pharmacology*; D012763:Shigella flexneri / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D014195:Travel; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015363:Quinolones