title: Protein interactions on telomeric retrotransposons in Drosophila.
abstract: Telomere length in Drosophila is maintained by targeted transposition of three non-LTR retrotransposons: HeT-A, TART and TAHRE (HTT), but understanding the regulation of this process is hindered by our poor knowledge of HTT associated proteins. We have identified new protein components of the HTT array: Chromator (Chro), the TRF2/DREF complex and the sumoylation machinery. Chro was localized on telomeric HTT arrays by immunostaining, where it may interact with Prod directly, as indicated by yeast two-hybrid interaction, co-IP, and colocalization on polytene chromosomes. The TRF2/DREF complex may promote the open structure of HTT chromatin. The protein interactions controlling HTT chromatin structure and telomere length may be modulated by sumoylation.
authors.affiliation: Department of Genetics, University of Szeged, H-6701 Szeged, Hungary.
authors.forename: Sándor
authors.initials: S
authors.lastname: Takács
authors.forename: Harald
authors.initials: H
authors.lastname: Biessmann
authors.forename: Hemakumar M
authors.initials: HM
authors.lastname: Reddy
authors.forename: James M
authors.initials: JM
authors.lastname: Mason
authors.forename: Tibor
authors.initials: T
authors.lastname: Török
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D047468:Immunoprecipitation; D016133:Polymerase Chain Reaction; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D018626:Retroelements / Q000235:genetics*; D016615:Telomere / Q000235:genetics*; D020798:Two-Hybrid System Techniques
chemical_list: D029721:Drosophila Proteins; D018626:Retroelements