title: Association of single nucleotide polymorphisms in the human tumor necrosis factor-α and interleukin 1-β genes in patients with pre-eclampsia.
abstract: Pre-eclampsia is a pregnancy-specific syndrome that may be dangerous especially to the fetus. Different cytokines have been found to be elevated in women with pre-eclampsia and may have possible roles in the development of this disorder. Alleles of the interleukin-l-beta (IL-lβ) and tumor necrosis factor alpha (TNF-α) genes are associated with pr-eeclampsia in several studies in different populations. The aim of the present study was to investigate the relationship between IL-lβ (C+3954T) and TNF-α (G-308A) gene polymorphisms with pre-eclampsia in north east of Iran (Khorasan province).This study included 54 diagnosed patients with pre-eclampsia and 50 normal pregnant women as control group. DNA was extracted from peripheral blood and the polymorphisms were determined by PCR-RFLP method. Data was analyzed using chi-square and Fisher's exact tests.There was significant association between TNF-α (G-308A) genotype and pre-eclampsia (p=0.001) but we did not find any significant association between IL-lβ (C+3954T) genotype and pre-eclampsia (p=0.39).The present study might suggest a role for TNF-α in the development of pre-eclampsia; however, IL-lβ (C+3954T) polymorphism could not be considered as a marker of susceptibility to preeclampsia in our population.
authors.affiliation: Department of Genetics, Ghaem Hospital, University of Medical Sciences, Mashhad, Iran.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Mohajertehran
authors.forename: Jalil
authors.initials: J
authors.lastname: Tavakkol Afshari
authors.forename: Zahra
authors.initials: Z
authors.lastname: Rezaieyazdi
authors.forename: Nayereh
authors.initials: N
authors.lastname: Ghomian
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D005091:Exons; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D007492:Iran / Q000453:epidemiology; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha