title: Inhibition of Hirame rhabdovirus growth by RNA aptamers.
abstract: RNA aptamers are artificial nucleic acids that specifically bind to a wide variety of targets. They are an effective tool for pharmaceutical research and development of antiviral agents. Here, we describe four Hirame rhabdovirus (HIRRV)-RNA aptamers (H1, H2, H3 and H4) that we obtained from an in vitro process called the systematic evolution of ligands by exponential enrichment (SELEX). The HIRRV-RNA aptamers specifically bind to HIRRV. Hirame natural embryo (HINAE) cells treated with virus and the RNA aptamer showed a decrease in appearance of cytopathic effect when compared with control (treated only with virus). Rhodovulum sulfidophilum was transformed with genes for the RNA aptamers, and the aptamers were detected in the culture medium, indicating that they were secreted from the cells. Thus, the recombinant R. sulfidophilum might be a powerful tool for the prevention of HIRRV in aquaculture.
authors.affiliation: Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Minato-ku, Tokyo, Japan.
authors.forename: S D
authors.initials: SD
authors.lastname: Hwang
authors.forename: N
authors.initials: N
authors.lastname: Midorikawa
authors.forename: P
authors.initials: P
authors.lastname: Punnarak
authors.forename: Y
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: H
authors.initials: H
authors.lastname: Kondo
authors.forename: I
authors.initials: I
authors.lastname: Hirono
authors.forename: T
authors.initials: T
authors.lastname: Aoki
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000096:biosynthesis / Q000378:metabolism* / Q000494:pharmacology*; D002460:Cell Line; D004274:DNA, Recombinant / Q000235:genetics; D005432:Flounder; D029141:Novirhabdovirus / Q000254:growth & development*; D010957:Plasmids / Q000235:genetics; D041903:Rhodovulum / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D052157:Aptamers, Nucleotide; D004274:DNA, Recombinant