title: [Adenovirus mediated N19RhoA gene transfer attenuates neointimal formation in rats after carotid artery balloon injury].
abstract: OBJECTIVE
  To investigate the role of small G-protein RhoA in neointimal formation following rat carotid artery balloon injury and related mechanisms.
  
  
  METHODS
  Male 3-4-month-old Sprague-Dawley rats were used in the present study (10 rats per group). Group A: control; Group B: carotid artery balloon injury; Group C: injury + Ad-CMV-eGFP + Pluronic F-127; Group D: injury + Ad-CMV-N19RhoA-eGFP + Pluronic F-127; Group E: non injury + Ad-CMV-eGFP + Pluronic F-127. Perivascular gene transfer of an adenovirus co-expressing N19RhoA was performed to rat carotid artery following balloon injury and the effect on neointimal formation and the expressions of PCNA and α-SM-actin examined. Rats were killed after 14 days.
  
  
  RESULTS
  The protein expression of RhoA in group B was significantly higher than in group A (P = 0.001), and the positive cells rate of PCNA and α-SM-actin which were assessed by immunohistochemistry in group C (45.2% and 75.6%) was significantly higher than in group D (28.4% and 51.9%, all P < 0.01). The area of neointima was significantly smaller [(0.14 ± 0.08) mm(2) vs. (0.23 ± 0.10) mm(2), P < 0.01], the luminal area was significantly larger [(0.47 ± 0.11) mm(2) vs. (0.31 ± 0.06) mm(2), P < 0.01] in group D than in group C.
  
  
  CONCLUSIONS
  Gene transfer of N19RhoA attenuates neointimal formation after balloon injury in rat carotid arteries possibly related to the modulating capacities of small G-protein RhoA on the proliferation, phenotypic differentiation and migration of vascular adventitial fibroblasts.
authors.affiliation: Department of Medical ICU, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Yu-feng
authors.initials: YF
authors.lastname: Chu
authors.forename: Wen-dong
authors.initials: WD
authors.lastname: Chen
authors.forename: Jin-jiao
authors.initials: JJ
authors.lastname: Jiang
authors.forename: Mei
authors.initials: M
authors.lastname: Meng
authors.forename: Juan
authors.initials: J
authors.lastname: Zeng
authors.forename: Chun-ting
authors.initials: CT
authors.lastname: Wang
authors.forename: Ping-jin
authors.initials: PJ
authors.lastname: Gao
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002339:Carotid Arteries / Q000378:metabolism; D020212:Carotid Artery Injuries / Q000378:metabolism / Q000473:pathology*; D005822:Genetic Vectors; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D058426:Neointima; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection; D020742:rhoA GTP-Binding Protein / Q000235:genetics*
chemical_list: D020742:rhoA GTP-Binding Protein