title: A freshwater streptomyces, isolated from Tyume River, produces a predominantly extracellular glycoprotein bioflocculant.
abstract: We evaluated bioflocculant production by a freshwater actinobacteria whose 16S rDNA nucleotide sequence was deposited in GenBank as Streptomyces sp. Gansen (accession number HQ537129). Optimum culture conditions for bioflocculant production were an initial medium pH of 6.8, incubation temperature of 30 °C, agitation speed of 160 rpm and an inoculum size of 2% (v/v) of cell density 1.5 × 10(8) cfu/mL. The carbon, nitrogen and cation sources for optimum bioflocculant production were glucose (89% flocculating activity), ammonium sulfate (76% flocculating activity) and MgCl(2). Bioflocculant pyrolysis showed three step decomposition indicative of three components while chemical analyses showed 78% carbohydrate and 22% protein (wt/wt). The mass ratio of neutral sugar, amino sugar and uronic acids was 4.6:2.4:3. FTIR spectrometry indicated the presence of carboxyl, hydroxyl and amino groups, typical for heteropolysaccharide. The bioflocculant showed a lattice structure as seen by SEM imaging. Its high flocculation activity suggests its suitability for industrial applicability.
authors.affiliation: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Uchechukwu U
authors.initials: UU
authors.lastname: Nwodo
authors.affiliation: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Mayowa O
authors.initials: MO
authors.lastname: Agunbiade
authors.affiliation: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Ezekiel
authors.initials: E
authors.lastname: Green
authors.affiliation: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Leonard V
authors.initials: LV
authors.lastname: Mabinya
authors.affiliation: Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa.
authors.forename: Anthony I
authors.identifier: 0000-0002-9770-085X
authors.initials: AI
authors.lastname: Okoh
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology*; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D006023:Glycoproteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S