title: Effects of an epidermal growth factor receptor-based cancer vaccine on wound healing and inflammation processes in murine experimental models.
abstract: Anti-epidermal growth factor receptor (EGFR) therapies have been proven clinically effective for a variety of epithelial tumours. Vaccination of mice with the extracellular domain (ECD) of autologous EGFR overcomes the tolerance to self-EGFR and has antimetastatic effect on EGFR+ tumour. Because EGF/EGFR-signalling plays an important role in the inflammation stage of wound healing, the main objective of this study was to explore the possible role of murine (m) EGFR-ECD vaccine in the croton-oil-induced ear oedema and wound healing process in mice as autologous experimental models, mimicking the possible post-surgical wound complication in patients treated with human EGFR-ECD/VSSP vaccine. Mice were intramuscularly immunised four times; biweekly with the mEGFR-ECD/VSSP/Mont. Seven days later, an 8 mm diameter, full-thickness skin wound was created on the back of each animal. Immunisation induced a strong specific humoral response against the mEGFR-ECD protein and a DTH dose-response curve but interestingly, animals treated with mEGFR-ECD/VSSP/Mont had similar inflammatory and healing speed responses compared to control ones. These data suggest that application of mEGFR-ECD/VSSP vaccine as a therapeutic approach in cancer patients could not elicit a poor healing process after surgery.
authors.affiliation: Biomodels Unit, National Center for Laboratory Animal Breeding, Finca Tirabeque, Boyeros, Havana, CubaCenter of Molecular Immunology, Atabey, Playa, Havana, Cuba.
authors.forename: Dasha
authors.initials: D
authors.lastname: Fuentes
authors.forename: Lewis
authors.initials: L
authors.lastname: Chacón
authors.forename: Angel
authors.initials: A
authors.lastname: Casacó
authors.forename: Nuris
authors.initials: N
authors.lastname: Ledón
authors.forename: Nidia
authors.initials: N
authors.lastname: Fernández
authors.forename: Arianna
authors.initials: A
authors.lastname: Iglesias
authors.forename: Diana R
authors.initials: DR
authors.lastname: Hernández
authors.forename: Belinda
authors.initials: B
authors.lastname: Sánchez
authors.forename: Rolando
authors.initials: R
authors.lastname: Pérez
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D019496:Cancer Vaccines / Q000627:therapeutic use*; D066246:ErbB Receptors / Q000276:immunology*; D005260:Female; D007249:Inflammation / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination; D014945:Wound Healing / Q000502:physiology*
chemical_list: D000906:Antibodies; D019496:Cancer Vaccines; D066246:ErbB Receptors