title: The cold adaptability of microorganisms with different carbon source in activated sludge treating synthetical wastewater.
abstract: The cold adaptability of microorganisms with different carbon source under 5°C was studied in activated sludge for treating synthetical wastewater. Phospholipid fatty acid (PLFA) analysis indicated contents of unsaturated fatty acids in cell membrane at 5°C were 13.66% and 24.96% higher for glucose and sodium acetate source than that at 25°C. PLFA biomarkers showed more Gram-negative bacteria enriched than Gram-positive bacteria in low-temperature activated sludge. The Shannon-Wiener diversity analysis demonstrated glucose fed reactor in low temperature had lower PLFA diversity index (1.21-1.30) than that at 25°C and sodium acetate source was reverse (1.08-0.69). The 16S rRNA analysis manifested certain microbes were considerably suitable for existence under cold environment, most of which belong to Gram-negative bacteria.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210093, Jiangsu, PR China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Niu
authors.forename: Jinju
authors.initials: J
authors.lastname: Geng
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Ren
authors.forename: Lili
authors.initials: L
authors.lastname: Ding
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
mesh_terms: D000220:Adaptation, Biological; D001419:Bacteria / Q000187:drug effects*; D001673:Biodegradation, Environmental / Q000187:drug effects; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000494:pharmacology*; D003080:Cold Temperature; D058645:Denaturing Gradient Gel Electrophoresis; D005227:Fatty Acids / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D016133:Polymerase Chain Reaction; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D013696:Temperature; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification
chemical_list: D002244:Carbon; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater