title: Whole genome analysis of human papillomavirus genotype 11 from cervix, larynx and lung.
abstract: The prevalence of human papillomavirus genotypes differs in various target organs. HPV16 is the most prevalent genotype in the cervix while genotypes 6 and 11 are highly prevalent in skin and aero-digestive tract infections. In this study HPV11 positive specimens were selected from cervix, larynx and lung biopsy tissue to analyze the whole genome by PCR and direct sequencing. Five HPV11 whole genomes were characterized, consisting of two cervical specimens, two laryngeal specimens and one lung specimen. The results showed high homology of HPV11 in these organs. Phylogenetic analysis showed that all HPV11 derived from various organs belonged to the same lineage. Molecular characterization and functional studies can further our understanding of virulence, expression or transmission. Additional studies on functional protein expression at different organ sites will also contribute to our knowledge of HPV infection in various organs.
authors.affiliation: Department of Paediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Jira
authors.initials: J
authors.lastname: Chansaenroj
authors.forename: Apiradee
authors.initials: A
authors.lastname: Theamboonlers
authors.forename: Pairoj
authors.initials: P
authors.lastname: Junyangdikul
authors.forename: Pakpoom
authors.initials: P
authors.lastname: Supiyaphan
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D000293:Adolescent; D000328:Adult; D001483:Base Sequence; D002584:Cervix Uteri / Q000821:virology*; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D005260:Female; D016679:Genome, Viral; D005838:Genotype; D052140:Human papillomavirus 11 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007830:Larynx / Q000821:virology*; D008168:Lung / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral