title: In vitro schistosomicidal activity of some brazilian cerrado species and their isolated compounds.
abstract: Miconia langsdorffii Cogn. (Melastomataceae), Roupala montana Aubl. (Proteaceae), Struthanthus syringifolius (Mart.) (Loranthaceae), and Schefflera vinosa (Cham. & Schltdl.) Frodin (Araliaceae) are plant species from the Brazilian Cerrado whose schistosomicidal potential has not yet been described. The crude extracts, fractions, the triterpenes betulin, oleanolic acid, ursolic acid and the flavonoids quercetin 3-O-β-D-rhamnoside, quercetin 3-O-β-D-glucoside, quercetin 3-O-β-D-glucopyranosyl-(1-2)-α-L-rhamnopyranoside and isorhamnetin 3-O-β-D-glucopyranosyl-(1-2)-α-L-rhamnopyranoside were evaluated in vitro against Schistosoma mansoni adult worms and the bioactive n-hexane fractions of the mentioned species were also analyzed by GC-MS. Betulin was able to cause worm death percentage values of 25% after 120 h (at 100 μM), and 25% and 50% after 24 and 120 h (at 200 μM), respectively; besides the flavonoid quercetin 3-O-β-D-rhamnoside promoted 25% of death of the parasites at 100 μM. Farther the flavonoids quercetin 3-O-β-D-glucoside and quercetin 3-O-β-D-rhamnoside at 100 μM exhibited significantly reduction in motor activity, 75% and 87.5%, respectively. Biological results indicated that crude extracts of R. montana, S. vinosa, and M. langsdorffii and some n-hexane and EtOAc fractions of this species were able to induce worm death to some extent. The results suggest that lupane-type triterpenes and flavonoid monoglycosides should be considered for further antiparasites studies.
authors.affiliation: Universidade de Franca, Avenida. Dr. Armando Salles de Oliveira 201, 14404-600 Franca, SP, Brazil.
authors.forename: Nayanne Larissa
authors.initials: NL
authors.lastname: Cunha
authors.forename: Camila Jacintho de Mendonça
authors.initials: CJ
authors.lastname: Uchôa
authors.forename: Lucas Silva
authors.initials: LS
authors.lastname: Cintra
authors.forename: Herbert Cristian
authors.initials: HC
authors.lastname: de Souza
authors.forename: Juliana Andrade
authors.initials: JA
authors.lastname: Peixoto
authors.forename: Claudia Peres
authors.initials: CP
authors.lastname: Silva
authors.forename: Lizandra Guidi
authors.initials: LG
authors.lastname: Magalhães
authors.forename: Valéria Maria Meleiro
authors.initials: VM
authors.lastname: Gimenez
authors.forename: Milton
authors.initials: M
authors.lastname: Groppo
authors.forename: Vanderlei
authors.initials: V
authors.lastname: Rodrigues
authors.forename: Ademar Alves
authors.initials: AA
authors.lastname: da Silva Filho
authors.forename: Márcio Luís
authors.initials: ML
authors.lastname: Andrade E Silva
authors.forename: Wilson Roberto
authors.initials: WR
authors.lastname: Cunha
authors.forename: Patrícia Mendonça
authors.initials: PM
authors.lastname: Pauletti
authors.forename: Ana Helena
authors.initials: AH
authors.lastname: Januário