title: Pathogen-induced accumulation of an ellagitannin elicits plant defense response.
abstract: In an incompatible interaction between Colletotrichum fragariae and strawberry plants, the accumulation of phenolic compounds in plant leaves was observed. A particularly abundant penta-esterified ellagitannin that accumulated in response to pathogen attack was identified as 1-0-galloyl-2,3;4,6-bis-hexahydroxydiphenoyl-β-d-glucopyranose (HeT) by mass spectroscopy and nuclear magnetic resonance. Foliar application of purified HeT prior to inoculation with a virulent pathogen was shown to increase resistance toward C. acutatum in strawberry plants and to Xanthomonas citri subsp. citri in lemon plants. The induced resistance in strawberry was associated with a rapid oxidative burst, callose deposition, a transient increase of salicylic acid in phloem, and induction of gene expression responsive to salicylic acid. Results obtained suggested that HeT could be a common plant defense response molecule capable of inducing pathogen resistance in different plant species.
authors.affiliation: Universidad Nacional de Tucuman, San Miguel de Tucuman, Argentina.
authors.forename: Alicia
authors.initials: A
authors.lastname: Mamaní
authors.forename: María Paula
authors.initials: MP
authors.lastname: Filippone
authors.forename: Carlos
authors.initials: C
authors.lastname: Grellet
authors.forename: Björn
authors.initials: B
authors.lastname: Welin
authors.forename: Atilio Pedro
authors.initials: AP
authors.lastname: Castagnaro
authors.forename: Juan Carlos Díaz
authors.initials: JC
authors.lastname: Ricci
mesh_terms: D002957:Citrus / Q000378:metabolism / Q000382:microbiology; D020231:Colletotrichum / Q000472:pathogenicity; D031985:Fragaria / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D047348:Hydrolyzable Tannins / Q000378:metabolism* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D052585:Phloem / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D014974:Xanthomonas / Q000472:pathogenicity
chemical_list: D047348:Hydrolyzable Tannins; C013515:ellagitannin; D020156:Salicylic Acid