title: [Influence of antimicrobial peptides of human thrombocytes on Staphylococcus aureus biofilm formation].
abstract: OBJECTIVE
  Study the influence ofplatelet low molecular weight protein on S. aureus biofilm formation.
  
  
  METHODS
  20 clinical isolates of coagulase-positive staphylococci (S. aureus) served as material. Preparation containing a mixture of antimicrobial peptides (fractions with molecular weight 60-70 kDa, 20-25 kDa and 5-10 kDa) obtained from human thrombocyte donors was used. Bio film formation (BFF) by staphylococci was studied photometrically, the degree of hydrophobicity was evaluated by separation of cell suspension in two-phase system liquid-liquid. The data obtained were treated by nonparametric method using Mann Whitney criteria.
  
  
  RESULTS
  Platelet low molecular weight proteins (PLB) at concentration of 2 microg/ml reduced biofilm formation of S. aureus by 48.7 +/- 9.8% from initial values, increased hydrophobicity of plankton and biofilm cell fractions. Maximum inhibitory effect of the preparation containing platelet low molecular weight proteins was noted at concentration of 50 microg/ml.
  
  
  CONCLUSIONS
  The data obtained on inhibition of staphylococci BFF by platelet low molecular weight protein open a perspective of further studies of PLB as a preparation suitable for control ofbiofilm of persistent microorganisms.
authors.forename: O S
authors.initials: OS
authors.lastname: Zhurlov
authors.forename: N B
authors.initials: NB
authors.lastname: Perunova
authors.forename: E V
authors.initials: EV
authors.lastname: Ivanova
authors.forename: O S
authors.initials: OS
authors.lastname: Egorova
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000032:analysis; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D001792:Blood Platelets / Q000737:chemistry*; D005591:Chemical Fractionation; D003030:Coagulase / Q000032:analysis; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D010933:Plankton / Q000187:drug effects* / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
chemical_list: D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; D003030:Coagulase