title: FRET-based ratiometric detection of Hg2+ and biothiols using naphthalimide-rhodamine dyads.
abstract: A new naphthalimide-rhodamine-based dyad in CH(3)CN-HEPES (1 : 1) buffer solution exhibits fluorescence resonance energy transfer (FRET) from naphthalimide to the rhodamine moiety on addition of only Hg(2+) ions and allows ratiometric absorption and fluorimetric estimation of Hg(2+) ions between 50 nM (10 ppb) to 2 μM (0.4 ppm). FRET-induced fluorescence changes were recovered again by the subsequent addition of thiol amino acids via reverse FRET. The interconversion of probe and via the complexation/decomplexation by the modulation of Hg(2+)/Cys exhibited a selective probe for biothiols in real samples.
authors.affiliation: School of Chemistry and Biochemistry, Thapar University, Patiala-147004, India. vj_luxami@yahoo.co.in
authors.forename: Vijay
authors.initials: V
authors.lastname: Luxami
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Verma
authors.forename: Richa
authors.initials: R
authors.lastname: Rani
authors.forename: Kamaldeep
authors.initials: K
authors.lastname: Paul
authors.forename: Subodh
authors.initials: S
authors.lastname: Kumar
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008628:Mercury / Q000032:analysis*; D015394:Molecular Structure; D053644:Naphthalimides / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D053644:Naphthalimides; D009942:Organometallic Compounds; D012235:Rhodamines; D013438:Sulfhydryl Compounds; D008628:Mercury