title: Assessment of acute moderate hyperglycemia on traditional and thromboelastometry coagulation parameters in healthy adult horses.
abstract: OBJECTIVE
  To determine whether experimentally induced acute moderate hyperglycemia is associated with coagulation activation in healthy adult horses.
  
  
  METHODS
  Prospective experimental study.
  
  
  METHODS
  University veterinary teaching hospital.
  
  
  METHODS
  Six healthy adult horses.
  
  
  METHODS
  Hyperglycemia (10.0-13.3 mmol/L [180-240 mg/dL]) was induced and maintained for 6 hours using a hyperglycemic clamp technique. Blood glucose concentrations were assessed using a point of care (POC) glucometer at 10- and 20-minute intervals throughout the hyperglycemic clamp procedure. Platelet count, fibrinogen concentration, prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin antithrombin complex level (TAT), and thromboelastometry (TEM) were determined before and after jugular catheter placement, prior to glucose administration, and at 3 and 6 hours of sustained hyperglycemia. Data were analyzed by repeated measures analysis of variance with significance defined as P < 0.05.
  
  
  RESULTS
  All horses maintained blood glucose concentration >10.0 mmol/L (>180 mg/dL) throughout the duration of the hyperglycemic clamp with a mean concentration of 11.9 ± 0.3 mmol/L [216 ± 6 mg/dL] as measured by the POC glucometer. No significant difference was found for any evaluated parameter associated with sustained hyperglycemia. Intravenous catheter placement resulted in a significant increase in mean TAT (0.8 ± 0.3 μg/L pre-catheter, 2.3 ± 0.8 μg/L post-catheter; P = 0.008).
  
  
  CONCLUSIONS
  Acute, moderate hyperglycemia in healthy adult horses does not have a detectable effect on coagulation based on evaluated parameters. Jugular catheter placement results in a transient increase in thrombin generation as determined by increased TAT concentrations.
authors.affiliation: College of Veterinary Medicine, University of Illinois, 1008 WHazelwood Drive, Urbana, IL 61802, USA.
authors.forename: Kate F
authors.initials: KF
authors.lastname: McGovern
authors.forename: Kara M
authors.initials: KM
authors.lastname: Lascola
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Smith
authors.forename: Stuart C
authors.initials: SC
authors.lastname: Clark-Price
authors.forename: Maureen
authors.initials: M
authors.lastname: McMichael
authors.forename: Pamela A
authors.initials: PA
authors.lastname: Wilkins
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D001786:Blood Glucose; D004305:Dose-Response Relationship, Drug; D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000633:toxicity*; D006734:Horse Diseases / Q000097:blood / Q000139:chemically induced*; D006736:Horses; D006943:Hyperglycemia / Q000139:chemically induced / Q000662:veterinary*; D007275:Injections, Intravenous; D008297:Male; D013916:Thrombelastography / Q000379:methods / Q000662:veterinary*
chemical_list: D001786:Blood Glucose; D005947:Glucose