title: Reversal of multidrug resistance in renal cell carcinoma by short hairpin RNA targeting MDR1 gene.
abstract: BACKGROUND
  Over-expression of P-glycoprotein (P-gp), encoded by the MDR1 gene, confers multidrug resistance (MDR) in renal cell carcinoma (RCC) and is a major reason for unsuccessful chemotherapy. This study aimed to determine the effct of RNA interference (RNAi) on the reversal of MDR in human RCC.
  
  
  METHODS
  We designed and selected one short hairpin RNA (shRNA) targeting MDR1 gene, which is stably expressed from integrated plasmid and transfected by lentivirus fluid in human RCC A498 cell.
  
  
  RESULTS
  The MDR1-targeted RNAi resulted in decreased MDR1 gene mRNA level (P < 0.001), almost abolished P-gp expression and reversed MDR to different chemotherapy drugs in the RCC A498 cell line.
  
  
  CONCLUSIONS
  MDR could be reversed by RNAi in human RCC A498 cell line, which may be used for clinical application in future.
authors.affiliation: Department of Oncology, First Affiliated Hospital of the People's Liberation Army General Hospital, Beijing 100048, China. hao.yixin@yahoo.com.cn
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Hao
authors.forename: Zheng-Wen
authors.initials: ZW
authors.lastname: He
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhu
authors.forename: Qian
authors.initials: Q
authors.lastname: Shen
authors.forename: Jun-Zhong
authors.initials: JZ
authors.lastname: Sun
authors.forename: Nan
authors.initials: N
authors.lastname: DU
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Xiao
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D020128:Inhibitory Concentration 50; D016086:Lentivirus / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D034741:RNA, Small Interfering