title: Inhibition of xyloglucanase from an alkalothermophilic Thermomonospora sp. by a peptidic aspartic protease inhibitor from Penicillium sp. VM24.
abstract: A bifunctional inhibitor from Penicillium sp VM24 causing inactivation of xyloglucanase from Thermomonospora sp and an aspartic protease from Aspergillus saitoi was identified. Steady state kinetics studies of xyloglucanase and the inhibitor revealed an irreversible, non-competitive, two-step inhibition mechanism with IC(50) and K(i) values of 780 and 500nM respectively. The interaction of o-phthalaldehyde (OPTA)-labeled xyloglucanase with the inhibitor revealed that the inhibitor binds to the active site of the enzyme. Far- and near-UV spectrophotometric analysis suggests that the conformational changes induced in xyloglucanase by the inhibitor may be due to irreversible denaturation of enzyme. The bifunctional inhibitor may have potential as a biocontrol agent for the protection of plants against phytopathogenic fungi.
authors.affiliation: Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, India.
authors.forename: Vishnu
authors.initials: V
authors.lastname: Menon
authors.forename: Mala
authors.initials: M
authors.lastname: Rao
mesh_terms: D000192:Actinomycetales / Q000201:enzymology*; D000468:Alkalies; D057055:Aspartic Acid Proteases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001230:Aspergillus / Q000201:enzymology*; D002942:Circular Dichroism; D005670:Fusarium / Q000187:drug effects; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007700:Kinetics; D016018:Least-Squares Analysis; D008826:Microbial Sensitivity Tests; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D010407:Penicillium / Q000737:chemistry*; D010455:Peptides / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D012233:Rhizopus / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D009764:o-Phthalaldehyde / Q000378:metabolism
chemical_list: D000468:Alkalies; D010455:Peptides; D011480:Protease Inhibitors; D009764:o-Phthalaldehyde; D006026:Glycoside Hydrolases; C072818:xyloglucan endo(1-4)-beta-D-glucanase; D057055:Aspartic Acid Proteases