title: Effect of photodynamic antimicrobial chemotherapy on in vitro and in situ biofilms.
abstract: OBJECTIVE
  The effect of photodynamic antimicrobial chemotherapy (PACT) on oral biofilms was evaluated.
  
  
  METHODS
  Biofilms formed in vitro were treated with sensitizer (S) and/or light (L) in the test (S+L+) and control (S-L-, S+L-, S-L+) groups. Additionally, 21 volunteers wore intraoral devices and the biofilms formed on each side of the device were referred to as S+L+ or S-L- groups.
  
  
  RESULTS
  Significant decreases in the viability of in vitro biofilms were observed after PACT. PACT was ineffective in inhibiting multi-species biofilms formed in situ.
  
  
  CONCLUSIONS
  PACT was effective on in vitro biofilms and was ineffective on in situ biofilms.
authors.affiliation: Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, Brazil.
authors.forename: A H
authors.initials: AH
authors.lastname: Teixeira
authors.forename: E S
authors.initials: ES
authors.lastname: Pereira
authors.forename: L K A
authors.initials: LK
authors.lastname: Rodrigues
authors.forename: D
authors.initials: D
authors.lastname: Saxena
authors.forename: S
authors.initials: S
authors.lastname: Duarte
authors.forename: I C J
authors.initials: IC
authors.lastname: Zanin
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D018441:Biofilms / Q000187:drug effects*; D003731:Dental Caries / Q000188:drug therapy* / Q000382:microbiology; D005260:Female; D006801:Humans; D008297:Male; D050296:Microbial Viability / Q000187:drug effects; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D016037:Single-Blind Method; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000187:drug effects*; D014048:Tolonium Chloride / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D017319:Photosensitizing Agents; D014048:Tolonium Chloride