title: Permeases of the sap transporter are required for cathelicidin resistance and virulence of Haemophilus ducreyi in humans.
abstract: BACKGROUND
  Haemophilus ducreyi encounters several classes of antimicrobial peptides (APs) in vivo and utilizes the sensitive-to-antimicrobial-peptides (Sap) transporter as one mechanism of AP resistance. A mutant lacking the periplasmic solute-binding component, SapA, was somewhat more sensitive to the cathelicidin LL-37 than the parent strain and was partially attenuated for virulence. The partial attenuation led us to question whether the transporter is fully abrogated in the sapA mutant.
  
  
  METHODS
  We generated a nonpolar sapBC mutant, which lacks both inner membrane permeases of the Sap transporter, and tested the mutant for virulence in human volunteers. In vitro, we compared LL-37 resistance phenotypes of the sapBC and sapA mutants.
  
  
  RESULTS
  Unlike the sapA mutant, the sapBC mutant was fully attenuated for virulence in human volunteers. In vitro, the sapBC mutant exhibited significantly greater sensitivity than the sapA mutant to killing by LL-37. Similar to the sapA mutant, the sapBC mutant did not affect H. ducreyi's resistance to human defensins.
  
  
  CONCLUSIONS
  Compared with the sapA mutant, the sapBC mutant exhibited greater attenuation in vivo, which directly correlated with increased sensitivity to LL-37 in vitro. These results strongly suggest that the SapBC channel retains activity when SapA is removed.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Sherri D
authors.initials: SD
authors.lastname: Rinker
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Gu
authors.forename: Kate R
authors.initials: KR
authors.lastname: Fortney
authors.forename: Beth W
authors.initials: BW
authors.lastname: Zwickl
authors.forename: Barry P
authors.initials: BP
authors.lastname: Katz
authors.forename: Diane M
authors.initials: DM
authors.lastname: Janowicz
authors.forename: Stanley M
authors.initials: SM
authors.lastname: Spinola
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Bauer
mesh_terms: D000328:Adult; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D002602:Chancroid / Q000382:microbiology / Q000473:pathology; D024881:Drug Resistance, Bacterial; D005260:Female; D017353:Gene Deletion; D006191:Haemophilus ducreyi / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D006805:Human Experimentation; D006801:Humans; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism*; D055815:Young Adult; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D026901:Membrane Transport Proteins; D037521:Virulence Factors; D054804:Cathelicidins