title: Flavonoids purified from parsley inhibit human blood platelet aggregation and adhesion to collagen under flow.
abstract: BACKGROUND
  Blood platelets are directly involved in both haemostatic and pathologic thrombotic processes, through their adhesion, secretion and aggregation. In this study, we investigated the effect of genins (aglycone flavonoids without sugar group) isolated from parsley (Petroselinum crispum) leaves in vitro on human platelet aggregation and adhesion to a collagen-coated surface under physiologic flow conditions.
  
  
  METHODS
  The aggregation and adhesion studies were monitored after pre-incubation of platelets with genins.
  
  
  RESULTS
  Genins inhibited dose dependently aggregation induced by thrombin, ADP and collagen. The strongest effect was observed in collagen induced aggregation (IC50 = 0.08 ± 0.01 mg/ml). The HPLC identification of genins compounds revealed the presence of keampferol, apigenin and other not identified compounds. The aggregation tests showed that these compounds have anti-aggregating activity. In addition, adhesion of human platelets to collagen was greatly decreased (over 75 %) by genins (0.3 mg/ml). While the mechanism by which genins act is unclear, we suggest that these compounds may interfere with a multiple target step in the haemostasis process.
  
  
  CONCLUSIONS
  These results show that genins isolated from parsley has a potent antiplatelet activity. It may be an important source of beneficial antiplatelet compounds that decrease thrombosis and cardiovascular diseases.
authors.affiliation: Université Mohammed Premier, Oujda.
authors.forename: Dounia
authors.initials: D
authors.lastname: Gadi
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bnouham
authors.forename: Mohammed
authors.initials: M
authors.lastname: Aziz
authors.forename: Abderrahim
authors.initials: A
authors.lastname: Ziyyat
authors.forename: Abdelkhaleq
authors.initials: A
authors.lastname: Legssyer
authors.forename: Arlette
authors.initials: A
authors.lastname: Bruel
authors.forename: Mohamed
authors.initials: M
authors.lastname: Berrabah
authors.forename: Chantal
authors.initials: C
authors.lastname: Legrand
authors.forename: Françoise
authors.initials: F
authors.lastname: Fauvel-Lafeve
authors.forename: Hassane
authors.initials: H
authors.lastname: Mekhfi
mesh_terms: D047310:Apigenin / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D024061:Collagen Type III; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D020128:Inhibitory Concentration 50; D016014:Linear Models; D028528:Petroselinum / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*
chemical_list: D024061:Collagen Type III; D005419:Flavonoids; D010936:Plant Extracts; D047310:Apigenin