title: Eucalyptus growth promotion by endophytic Bacillus spp.
abstract: Clonal eucalyptus plantings have increased in recent years; however,  some clones with high production characteristics have vegetative propagation  problems because of weak root and aerial development. Endophytic microorganisms  live inside healthy plants without causing any damage to their hosts and can be  beneficial, acting as plant growth promoters. We isolated endophytic bacteria  from eucalyptus plants and evaluated their potential in plant growth promotion  of clonal plantlets of Eucalyptus urophylla x E. grandis, known  as the hybrid, E. urograndis. Eighteen isolates of E. urograndis,  clone 4622, were tested for plant growth promotion using the same clone. These  isolates were also evaluated for indole acetic acid production and their  potential for nitrogen fixation and phosphate solubilization. The isolates were  identified by partial sequencing of 16S rRNA. Bacillus subtilis was the  most prevalent species. Several Bacillus species, including B.  licheniformis and B. subtilis, were found for the first time as  endophytes of eucalyptus. Bacillus sp strain EUCB 10 significantly  increased the growth of the root and aerial parts of eucalyptus plantlets under  greenhouse conditions, during the summer and winter seasons.
authors.affiliation: Laboratório de Microbiologia Fitopatógica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil. isapaz@gmail.com
authors.forename: I C P
authors.initials: IC
authors.lastname: Paz
authors.forename: R C M
authors.initials: RC
authors.lastname: Santin
authors.forename: A M
authors.initials: AM
authors.lastname: Guimarães
authors.forename: O P P
authors.initials: OP
authors.lastname: Rosa
authors.forename: A C F
authors.initials: AC
authors.lastname: Dias
authors.forename: M C
authors.initials: MC
authors.lastname: Quecine
authors.forename: J L
authors.initials: JL
authors.lastname: Azevedo
authors.forename: A T S
authors.initials: AT
authors.lastname: Matsumura
mesh_terms: D001407:Bacillus / Q000302:isolation & purification / Q000502:physiology*; D018533:Biomass; D060026:Endophytes / Q000302:isolation & purification / Q000502:physiology*; D005052:Eucalyptus / Q000254:growth & development* / Q000382:microbiology*; D006824:Hybridization, Genetic; D007210:Indoleacetic Acids / Q000378:metabolism; D009586:Nitrogen Fixation; D010710:Phosphates / Q000378:metabolism; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D018547:Plant Stems / Q000382:microbiology; D012995:Solubility
chemical_list: D007210:Indoleacetic Acids; D010710:Phosphates; C030737:indoleacetic acid