title: Accelerating animal cell growth in perfusion mode by multivariable control: simulation studies.
abstract: This study considers the problem of manipulating in an optimal way the perfusion and bleed flow rates of a continuous culture of hybridoma cells, so as to achieve a fast transient start-up and reject potential disturbances. The proposed solution makes use of an analysis of the properties of the steady state solutions of the nonlinear dynamic model of the cell culture, and in particular the relationship between the two main limiting substrates, glucose and glutamine. The solution is implemented using extended prediction self-adaptive control. Simulation results demonstrate the approach potentiality.
authors.affiliation: Automatic Control Laboratory, University of Mons, 31 Boulevard Dolez, 7000, Mons, Belgium. MihaelaIuliana.Sbarciog@Umons.ac.be
authors.forename: Mihaela
authors.initials: M
authors.lastname: Sbarciog
authors.forename: Ines
authors.initials: I
authors.lastname: Saraiva
authors.forename: Alain
authors.initials: A
authors.lastname: Vande Wouwer
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D003198:Computer Simulation; D006825:Hybridomas / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological