title: A fluorescence switch sensor for 6-mercaptopurine detection based on gold nanoparticles stabilized by biomacromolecule.
abstract: It is of great significance to develop a simple and powerful assay of 6-mercaptopurine (6-MP) because of its serious side effect and variable activity with the plasma concentration. In this contribution, a fluorescence switch sensor for trace amount detection of 6-MP was successfully developed based on the fluorescent gold nanoparticles stabilized by macromolecules. With the turn-off and on of the fluorescence signal at 640 nm of the analytical system, the selectivity of the present assay was largely improved. Trace amount of 6-MP could be detected in the linear range 1.0×10(-7) M-1.2×10(-4) M with a detection limit 1.98×10(-8) M. Under a UV lamp, the color change with the variation of the 6-MP concentration could be seen clearly by naked eyes. The sensitivity and selectivity are several-fold greater than other methods. And also it proved to be able to detect trace amount of 6-MP in real samples. The present assay largely improved the application of spectral methods in quantitative analysis of 6-MP.
authors.affiliation: Department of Chemistry, Shantou University, Shantou 515063, China. kqlu@stu.edu.cn
authors.forename: Zhanguang
authors.initials: Z
authors.lastname: Chen
authors.forename: Guomin
authors.initials: G
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Junhui
authors.initials: J
authors.lastname: Chen
authors.forename: Jinbin
authors.initials: J
authors.lastname: Liu
authors.forename: Haiqiong
authors.initials: H
authors.lastname: Yuan
mesh_terms: D001704:Biopolymers / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D015122:Mercaptopurine / Q000032:analysis* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D001704:Biopolymers; D006046:Gold; D015122:Mercaptopurine