title: [Polymorphism of XRCC1 and chromosome damage in workers occupationally exposed to benzene].
abstract: OBJECTIVE
  To explore the relationship between the polymorphisms of DNA repair gene (XRCC1 194, 280 and 399) and the chromosomal damage induced by benzene.
  
  
  METHODS
  The chromosomal damage of the peripheral lymphocytes in 459 workers occupationally exposed to benzene and 88 non-exposed controls were detected with cytokinesis-block micronucleus (CBMN) assay. PCR-RFLP technique was used to measure polymorphisms in XRCC1 194, 280 and 399.
  
  
  RESULTS
  It was found that the MN frequency (2.12‰ ± 1.88‰) of the exposed group was significantly higher than that (1.19‰ ± 1.68‰) of the control group (P < 0.05), in the exposed group, the MN frequency (3.00‰ ± 2.76‰) of older workers (> 35 years) was significantly higher than that (2.02‰ ± 1.71‰) of younger workers (≤ 35 years) (P < 0.05). The effect of genetic polymorphisms of XRCC1 on CBMN was not found. The haplotypes AAA/BAA, AAB/AAB, ABA/ABA, ABB/ABB could associated with the increased frequencies of total micronucleus (P < 0.05).
  
  
  CONCLUSIONS
  Benzene exposure could result in chromosome damage. Age of workers and diplotypes of XRCC1 could associated with chromosomal damage induced by benzene.
authors.affiliation: Affiliated of tumor hospital, Xinjiang Medical University, Urumqi 830011, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jian-ping
authors.initials: JP
authors.lastname: Lü
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.forename: Li-fang
authors.initials: LF
authors.lastname: Zhou
authors.forename: Yun-jie
authors.initials: YJ
authors.lastname: Ye
authors.forename: Pin
authors.initials: P
authors.lastname: Sun
authors.forename: Zhou-xiang
authors.initials: ZX
authors.lastname: Cheng
authors.forename: Zhao-lin
authors.initials: ZL
authors.lastname: Xia
mesh_terms: D000328:Adult; D001554:Benzene / Q000009:adverse effects*; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D048629:Micronuclei, Chromosome-Defective; D015162:Micronucleus Tests; D016273:Occupational Exposure; D020641:Polymorphism, Single Nucleotide; D000076105:X-ray Repair Cross Complementing Protein 1; D055815:Young Adult
chemical_list: D004268:DNA-Binding Proteins; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; D001554:Benzene