title: [Clinically relevant sepsis model in minipigs].
abstract: OBJECTIVE
  Our aim was to develop a large animal model of sepsis induced by fecal peritonitis, which reproduces the characteristic macrohemodynamic, microcirculatory and inflammatory changes seen in human sepsis.
  
  
  METHODS
  Anesthetized minipigs were subjected to fecal peritonitis (n = 9; 0.5 g/kg i.p. autofeces) or sham-operation (i.p. saline, n = 6). Invasive hemodynamic monitoring was started with regular blood gas analyses between the 15-24 hr of the insult. Sublingual microcirculation was characterized by red blood cell velocity changes (with orthogonal polarization spectral imaging), and the extravascular lung water index (EVLWI) was measured. The plasma levels of big-endothelin (big-ET) and high-mobility group box protein-1 (HMGB1) were determined from venous blood samples.
  
  
  RESULTS
  The mean arterial pressure gradually decreased below 70 mmHg in septic animals, while the heart rate and cardiac output increased constantly. In spite of the hyperdynamic reaction, significant elevation of the EVLWI was observed, while the sublingual microcirculation deteriorated, as compared with the control group. The big-ET and HMGB1 plasma concentrations were significantly elevated between 6-24 hr of peritonitis.
  
  
  CONCLUSIONS
  The in vivo data suggest that our fecal peritonitis-induced experimental sepsis model is of clinical relevance, and may play useful roles in the development of novel, sepsis-related therapies.
authors.affiliation: Szegedi Tudományegyetem, Általános Orvostudományi Kar Sebészeti Műtéttani Intézet 6720 Szeged Pécsi u. 6.
authors.forename: Bettina
authors.initials: B
authors.lastname: Zsikai
authors.forename: Lajos
authors.initials: L
authors.lastname: Bizánc
authors.forename: Péter
authors.initials: P
authors.lastname: Sztányi
authors.forename: Gergely
authors.initials: G
authors.lastname: Vida
authors.forename: Enikő
authors.initials: E
authors.lastname: Nagy
authors.forename: Lucian
authors.initials: L
authors.lastname: Jiga
authors.forename: Mihai
authors.initials: M
authors.lastname: Ionac
authors.forename: Dániel
authors.initials: D
authors.lastname: Erces
authors.forename: Mihály
authors.initials: M
authors.lastname: Boros
authors.forename: József
authors.initials: J
authors.lastname: Kaszaki
mesh_terms: D000818:Animals; D062186:Arterial Pressure; D015415:Biomarkers / Q000097:blood; D001783:Blood Flow Velocity; D002302:Cardiac Output; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000097:blood; D005243:Feces; D024243:HMGB1 Protein / Q000097:blood; D006339:Heart Rate; D006439:Hemodynamics; D007249:Inflammation; D008833:Microcirculation; D010538:Peritonitis / Q000097:blood / Q000150:complications* / Q000473:pathology / Q000503:physiopathology*; D018805:Sepsis / Q000097:blood / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D013552:Swine; D013556:Swine, Miniature; D013997:Time Factors
chemical_list: D015415:Biomarkers; D019332:Endothelin-1; D024243:HMGB1 Protein