title: Antipsoriatic activity of extracts and fractions obtained from Memecylon malabaricum leaves.
abstract: OBJECTIVE
  This study involves the evaluation of Memecylon malabaricum Cogn. (Melastomataceae) leaves for antipsoriatic activity.
  
  
  METHODS
  Aqueous extract, hydroalcoholic extract and their fractions of M. malabaricum leaves were evaluated for in-vivo antipsoriatic activity by mouse tail test and for in-vitro antipsoriatic activity using HaCaT cells, lipoxygenase inhibition and thymidine phosphorylase inhibition assays. Extracts and fractions were evaluated for total phenol and flavonoid contents. HPTLC was used for screening and fingerprint analysis of the extracts and active fraction.
  
  
  RESULTS
  M. malabaricum hydroalcoholic extract (MMHA) and water fraction of MMHA (MMHAW) produced significant (P<0.05) percent orthokeratosis in the mouse tail test. All samples except MMHA showed a significant (P < 0.05) reduction in epidermal thickness in the mouse tail test when compared with control. Maximum activity against HaCaT cells was shown by chloroform fraction of MMHA (MMHAC). The M. malabaricum decoction (MMD) and water fraction of MMD (MMDW) showed equally good inhibition of lipoxygenase. In thymidine phosphorylase inhibition assay only MMD showed activity.
  
  
  CONCLUSIONS
  The findings of this investigation reveal that the leaves of M. malabaricum have good antipsoriatic potential, which provides scope for further detailed research in to this plant for psoriasis.
authors.affiliation: Department of Phytopharmacy and Phytomedicine, (TIFAC CORE HD), JSS Medical College, Mysore, India. dhanabalsp@rediffmail.com
authors.forename: Sangai Palanisamy
authors.initials: SP
authors.lastname: Dhanabal
authors.forename: Nithyanantham
authors.initials: N
authors.lastname: Muruganantham
authors.forename: Kabbur Hanumanthappa
authors.initials: KH
authors.lastname: Basavaraj
authors.forename: Asish
authors.initials: A
authors.lastname: Wadhwani
authors.forename: Nonavinakere Mannar
authors.initials: NM
authors.lastname: Shamasundar
mesh_terms: D000818:Animals; D002460:Cell Line; D002855:Chromatography, Thin Layer; D003879:Dermatologic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004195:Disease Models, Animal; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D016859:Lipoxygenase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D035322:Melastomataceae / Q000737:chemistry*; D051379:Mice; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D011565:Psoriasis / Q000188:drug therapy* / Q000473:pathology
chemical_list: D003879:Dermatologic Agents; D005419:Flavonoids; D016859:Lipoxygenase Inhibitors; D010636:Phenols; D010936:Plant Extracts