title: [Influence of CO2 pneumoperitoneum pressures on the expression of adhesion molecules of gastric cancer cells].
abstract: OBJECTIVE
  To study the effect of different CO2 pneumoperitoneum pressures on the expression of adhesion molecules of human gastric cancer cell line MNK-45.
  
  
  METHODS
  MKN-45 cells in the experimental groups were exposed to simulated CO2 environment maintained at different pressures (1.2, 1.6, 2.0 kPa) for 4 hours. Control groups were exposed to room air. At the 0, 24, 48, 72, 96 hours after treatment, CD44v6, ICAM-1 and E-cadherin were detected by flow cytometry method.
  
  
  RESULTS
  CD44v6 and ICAM-1 expressions showed pattern of firstly elevating, then descending to normal under the pressures of 1.2 kPa and 1.6 kPa. The expressions were different from control group significantly at 24 and 48 hours (P<0.01), while the 72 hours expression showed no difference compared with the controls (P>0.05). E-cadherin expression decreased significantly right after treatment compared to the control (P<0.01), but recovered to the level of control at 48 hours (P>0.05). In the 2.0 kPa group the expression changes of CD44v6, ICAM-1 and E-cadherin were more remarkable. CD44v6 and ICAM-1 expressions were increased significantly compared to control right after treatment (P<0.05). E-cadherin expression was significantly decreased even at 48 hours compared to the controls (P<0.01).
  
  
  CONCLUSIONS
  In vitro CO2 pneumoperitoneum pressures have transient influence on the adhesion molecules expression of gastric cancer cell MKN-45, then those expressions can recover in a short-time.
authors.affiliation: Department of General Surgery, Center of Microinvasive Gastrointestinal Surgery, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Pei-wu
authors.initials: PW
authors.lastname: Yu
authors.forename: Xiao
authors.initials: X
authors.lastname: Lei
authors.forename: Feng
authors.initials: F
authors.lastname: Qian
authors.forename: Yong-liang
authors.initials: YL
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
authors.forename: Ying-xue
authors.initials: YX
authors.lastname: Hao
authors.forename: Peng
authors.initials: P
authors.lastname: Yin
authors.forename: Jing
authors.initials: J
authors.lastname: Li
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002245:Carbon Dioxide; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D011028:Pneumoperitoneum, Artificial; D011312:Pressure; D013274:Stomach Neoplasms / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C112697:CD44v6 antigen; C513384:CDH1 protein, human; D015820:Cadherins; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D018799:Intercellular Adhesion Molecule-1; D002245:Carbon Dioxide