title: Psychoneuroimmunology and natural killer cells: the chromium release whole blood assay.
abstract: Natural killer (NK) cells are an essential component of innate immunity. These lymphocytes are also sensitive barometers of the effects of endogenous and exogenous stressors on the immune system. This chapter will describe a chromium ((51)Cr) release bioassay designed to measure the target cell killing capacity of NK cells (NKCC). Key features of the cytotoxicity assay are that it is done with whole blood and that numbers of effector cells are determined for each sample by flow cytometry and lymphocyte count. Effector cells are defined as CD3-CD56+ lymphocytes. Target cells are the K562 eyrthroleukemia cell line. Killing capacity is defined as number of target cells killed per effector cell, at an effector cell/target cell ratio of 1:1 during a 4 h in vitro assay.
authors.affiliation: Department of Medicine, University of Miami, Miami, FL, USA. MFletche@med.miami.edu
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Fletcher
authors.forename: Zachary
authors.initials: Z
authors.lastname: Barnes
authors.forename: Gordon
authors.initials: G
authors.lastname: Broderick
authors.forename: Nancy G
authors.initials: NG
authors.lastname: Klimas
mesh_terms: D017252:CD3 Complex / Q000276:immunology; D019002:CD56 Antigen / Q000276:immunology; D002857:Chromium / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D020014:K562 Cells; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D018655:Lymphocyte Count; D011598:Psychoneuroimmunology / Q000379:methods
chemical_list: D017252:CD3 Complex; D019002:CD56 Antigen; D002857:Chromium