title: A novel locus for autosomal dominant cone-rod dystrophy maps to chromosome 10q.
abstract: Here we report recruitment of a three-generation Romani (Gypsy) family with autosomal dominant cone-rod dystrophy (adCORD). Involvement of known adCORD genes was excluded by microsatellite (STR) genotyping and linkage analysis. Subsequently, two independent total-genome scans using STR markers and single-nucleotide polymorphisms (SNPs) were performed. Haplotype analysis revealed a single 6.7-Mb novel locus between markers D10S1757 and D10S1782 linked to the disease phenotype on chromosome 10q26. Linkage analysis gave a maximum LOD score of 3.31 for five fully informative STR markers within the linked interval corresponding to the expected maximum in the family. Multipoint linkage analysis of SNP genotypes yielded a maximum parametric linkage score of 2.71 with markers located in the same chromosomal interval. There is no previously mapped CORD locus in this interval, and therefore the data reported here is novel and likely to identify a new gene that may eventually contribute to new knowledge on the pathogenesis of this condition. Sequencing of several candidate genes within the mapped interval led to negative findings in terms of the underlying molecular pathogenesis of the disease in the family. Analysis by comparative genomic hybridization excluded large chromosomal aberrations as causative of adCORD in the pedigree.
authors.affiliation: Department of Cellular Therapy and Regenerative Medicine, CSIC-CABIMER, Seville, Spain.
authors.forename: Kunka
authors.initials: K
authors.lastname: Kamenarova
authors.forename: Sylvia
authors.initials: S
authors.lastname: Cherninkova
authors.forename: Margarita
authors.initials: M
authors.lastname: Romero Durán
authors.forename: DeQuincy
authors.initials: D
authors.lastname: Prescott
authors.forename: Maria Lourdes
authors.initials: ML
authors.lastname: Valdés Sánchez
authors.forename: Vanio
authors.initials: V
authors.lastname: Mitev
authors.forename: Ivo
authors.initials: I
authors.lastname: Kremensky
authors.forename: Radka
authors.initials: R
authors.lastname: Kaneva
authors.forename: Shomi S
authors.initials: SS
authors.lastname: Bhattacharya
authors.forename: Ivailo
authors.initials: I
authors.lastname: Tournev
authors.forename: Christina
authors.initials: C
authors.lastname: Chakarova
mesh_terms: D002879:Chromosomes, Human, Pair 10; D005260:Female; D005799:Genes, Dominant; D008040:Genetic Linkage; D056426:Genetic Loci; D020022:Genetic Predisposition to Disease; D006801:Humans; D008126:Lod Score; D008297:Male; D018895:Microsatellite Repeats; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide; D012174:Retinitis Pigmentosa / Q000235:genetics*; D012383:Romania / Q000208:ethnology