title: Clinical transplantation of ex vivo expanded autologous limbal epithelial cells using a culture medium with human serum as single supplement: a retrospective case series.
abstract: OBJECTIVE
  Presently, our clinic is the only centre in Scandinavia that offers patients with corneal surface pathology including limbal stem cell deficiency (LSCD) transplantation of ex vivo expanded limbal epithelial cells (LECs). We here present clinical data of the first nine patients with LSCD who were transplanted with autologous LECs expanded in medium completely free of any animal-derived products and non-human/recombinant growth factors (including Cholera Toxin), and with autologous human serum as the only growth supplement.
  
  
  METHODS
  We conducted a noncomparative retrospective study of patients with LSCD at our centre between 2009 and 2011. The diagnosis was based on history and clinical signs. A biopsy was taken from healthy limbus, and the epithelium was expanded on amniotic membrane (AM) in medium containing autologous serum and subsequently transplanted to the affected eye.
  
  
  RESULTS
  Successful outcome was defined as relief of pain and photophobia and/or improved best corrected visual acuity (BCVA) and/or reestablishment of a stable corneal epithelium and regression of corneal vascularization. Five of the nine transplanted patients (55.6%) had an improvement in either subjective symptoms or objective findings (11- to 28-month follow-up).
  
  
  CONCLUSIONS
  Our clinical study shows that patients with LSCD can be treated successfully with transplantation of LECs expanded ex vivo in a medium with autologous serum as the only growth supplement. The use of this novel culture system, which is devoid of animal-derived products and non-human/recombinant growth factors (including Cholera Toxin), reduces the risks of inter-species disease transmission and host immune responses to xenogenic proteins, both obvious advantages for the patient.
authors.affiliation: Center for Eye Research and the Norwegian Eye Bank, Department of Ophthalmology, Oslo University Hospital Ullevål and University of Oslo, Oslo, NorwayDepartment of Ophthalmology, Oslo University Hospital Ullevål, Oslo, Norway.
authors.forename: Meeta
authors.initials: M
authors.lastname: Pathak
authors.forename: Symira
authors.initials: S
authors.lastname: Cholidis
authors.forename: Kristiane
authors.initials: K
authors.lastname: Haug
authors.forename: Aboulghassem
authors.initials: A
authors.lastname: Shahdadfar
authors.forename: Morten C
authors.initials: MC
authors.lastname: Moe
authors.forename: Bjørn
authors.initials: B
authors.lastname: Nicolaissen
authors.forename: Liv
authors.initials: L
authors.lastname: Drolsum
mesh_terms: D000328:Adult; D000368:Aged; D018929:Cell Culture Techniques / Q000379:methods*; D017690:Cell Transplantation / Q000379:methods; D002648:Child; D003316:Corneal Diseases / Q000503:physiopathology / Q000601:surgery*; D003470:Culture Media; D004847:Epithelial Cells / Q000166:cytology; D019573:Epithelium, Corneal / Q000166:cytology*; D005260:Female; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D044967:Serum / Q000502:physiology*; D033581:Stem Cell Transplantation; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014792:Visual Acuity / Q000502:physiology; D055815:Young Adult
chemical_list: D003470:Culture Media