title: Evaluation of novel decellularizing corneal stroma for cornea tissue engineering applications.
abstract: OBJECTIVE
  To develop a new decellularization method depended upon the natural corneal structure and to harvest an ideal scaffold with good biocompatibilities for corneal reconstruction.
  
  
  METHODS
  The acellular cornea matrix (ACM) were prepared from de-epithelium fresh porcine corneas (DFPCs) by incubation with 100% fresh human sera and additional electrophoresis at 4°C. Human corneal epithelial cells (HCEs) were used for the cytotoxicity tests of ACM. ACM were implanted into the Enhanced Green Fluorecence Protein (eGFP) transgenic mouse anterior chamber for evaluation of histocompatibility.
  
  
  RESULTS
  HE and GSIB4 results showed fresh porcine cornea matrix with 100% human sera and electrophoresis could entirely decellularize stromal cell without reducing its transparency. ACM had no cytotoxic effect ex vivo. Animal test showed there was no rejection for one month after surgery.
  
  
  CONCLUSIONS
  These results provide a decellularizing approach for the study of corneal tissue engineering and had the broader implications for the field of biological tissue engineering in other engineered organ or tissue matrix.
authors.affiliation: Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shao
authors.forename: Yao
authors.initials: Y
authors.lastname: Yu
authors.forename: Chong-Gang
authors.initials: CG
authors.lastname: Pei
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhou
authors.forename: Qiu-Ping
authors.initials: QP
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Tan
authors.forename: Jing-Ming
authors.initials: JM
authors.lastname: Li
authors.forename: Gui-Ping
authors.initials: GP
authors.lastname: Gao
authors.forename: Lu
authors.initials: L
authors.lastname: Yang