title: Use of gene targeting to study recombination in mammalian cell DNA repair mutants.
abstract: The study of gene function has been greatly facilitated by the development of strategies to modify genomic DNA. Gene targeting is one of the most successfully applied techniques used to examine the roles of specific genes in a wide variety of model systems from yeast to mammals. Our laboratory has pioneered the use of the Chinese hamster ovary (CHO) cell culture model system to study pathways of DNA repair and recombination at the hemizygous CHO APRT locus. By using a simple and effective gene targeting method, we have generated a number of DNA repair-deficient cell lines that have been used in targeted recombination experiments to investigate pathways of recombinational repair in somatic mammalian cells. These methods can be readily customized to generate a variety of cell lines deficient in specific genes of interest and can be applied to study the roles of other DNA repair proteins in pathways of mammalian recombinational repair.
authors.affiliation: Department of Molecular Carcinogenesis, Medical University of South Carolina, Charleston, SC, USA.
authors.forename: Jennifer J
authors.initials: JJ
authors.lastname: Rahn
authors.forename: Gerald M
authors.initials: GM
authors.lastname: Adair
authors.forename: Rodney S
authors.initials: RS
authors.lastname: Nairn
mesh_terms: D000228:Adenine Phosphoribosyltransferase / Q000172:deficiency / Q000235:genetics; D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair; D018274:Electroporation; D055786:Gene Knockout Techniques; D018390:Gene Targeting / Q000379:methods*; D056426:Genetic Loci / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D009154:Mutation; D011995:Recombination, Genetic
chemical_list: D000228:Adenine Phosphoribosyltransferase