title: Renal liver-type fatty acid binding protein attenuates angiotensin II-induced renal injury.
abstract: To investigate the role of human liver-type fatty acid binding protein (hL-FABP) in angiotensin (Ang) II-induced renal injury, Ang II was infused systemically into hL-FABP chromosomal transgenic (Tg) and wild-type (WT) mice (Tg-Ang II and WT-Ang II) for 28 days. Control mice were injected with saline only (Tg-control and WT-control). hL-FABP was expressed in proximal tubules of Tg mice. After a high-dose injection of Ang II, renal gene and protein expressions of hL-FABP in Tg-Ang II mice increased significantly compared with Tg-control mice. Urinary excretion of L-FABP was significantly greater in Tg-Ang II than in Tg-control mice. Blood pressure levels in both groups increased to a similar extent. Upregulation of monocyte chemoattractant protein 1 expression, macrophage infiltration in the interstitium, tubulointerstitial damage, and depositions of type I and III collagens were observed in both Tg-Ang II and WT-Ang II mice. However, these effects were less pronounced in Tg-Ang II compared with WT-Ang II mice. The level of renal N-(hexanoyl)lysine, an oxidative stress marker, was significantly higher in WT-Ang II than in Tg-Ang II mice. In conclusion, renal hL-FABP reduced oxidative stress in Ang II-induced renal injury and attenuated tubulointerstitial damage.
authors.affiliation: Division of Nephrology and Hypertension, Department of Internal Medicine, St Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-Ku, Kawasaki 216-8511, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ichikawa
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kamijo-Ikemori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sugaya
authors.forename: Takashi
authors.initials: T
authors.lastname: Yasuda
authors.forename: Seiko
authors.initials: S
authors.lastname: Hoshino
authors.forename: Jyunko
authors.initials: J
authors.lastname: Igarashi-Migitaka
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Hirata
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Kimura
mesh_terms: D000804:Angiotensin II / Q000502:physiology*; D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009400:Nephrosclerosis / Q000139:chemically induced* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D018932:Chemokine CCL2; C493319:FABP1 protein, human; D050556:Fatty Acid-Binding Proteins; D000804:Angiotensin II