title: Large-scale MHC class II genotyping of a wild lemur population by next generation sequencing.
abstract: The critical role of major histocompatibility complex (MHC) genes in disease resistance, along with their putative function in sexual selection, reproduction and chemical ecology, make them an important genetic system in evolutionary ecology. Studying selective pressures acting on MHC genes in the wild nevertheless requires population-wide genotyping, which has long been challenging because of their extensive polymorphism. Here, we report on large-scale genotyping of the MHC class II loci of the grey mouse lemur (Microcebus murinus) from a wild population in western Madagascar. The second exons from MHC-DRB and -DQB of 772 and 672 individuals were sequenced, respectively, using a 454 sequencing platform, generating more than 800,000 reads. Sequence analysis, through a stepwise variant validation procedure, allowed reliable typing of more than 600 individuals. The quality of our genotyping was evaluated through three independent methods, namely genotyping the same individuals by both cloning and 454 sequencing, running duplicates, and comparing parent-offspring dyads; each displaying very high accuracy. A total of 61 (including 20 new) and 60 (including 53 new) alleles were detected at DRB and DQB genes, respectively. Both loci were non-duplicated, in tight linkage disequilibrium and in Hardy-Weinberg equilibrium, despite the fact that sequence analysis revealed clear evidence of historical selection. Our results highlight the potential of 454 sequencing technology in attempts to investigate patterns of selection shaping MHC variation in contemporary populations. The power of this approach will nevertheless be conditional upon strict quality control of the genotyping data.
authors.affiliation: Behavioral Ecology and Sociobiology Unit, German Primate Center, Kellnerweg 4, Göttingen, Germany. ehuchard@gmail.com
authors.forename: Elise
authors.initials: E
authors.lastname: Huchard
authors.forename: Christina
authors.initials: C
authors.lastname: Albrecht
authors.forename: Susanne
authors.initials: S
authors.lastname: Schliehe-Diecks
authors.forename: Alice
authors.initials: A
authors.lastname: Baniel
authors.forename: Christian
authors.initials: C
authors.lastname: Roos
authors.forename: Peter M
authors.initials: PM
authors.lastname: Kappeler
authors.forename: Peter M Kappeler
authors.initials: PM
authors.lastname: Peter
authors.forename: Markus
authors.initials: M
authors.lastname: Brameier
mesh_terms: D000818:Animals; D001483:Base Sequence; D005091:Exons; D005802:Genes, MHC Class II / Q000235:genetics*; D056426:Genetic Loci; D060005:Genotyping Techniques / Q000379:methods*; D007900:Lemur / Q000235:genetics* / Q000276:immunology; D015810:Linkage Disequilibrium; D008270:Madagascar; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011786:Quality Control; D017422:Sequence Analysis, DNA / Q000379:methods*