title: Probing translation initiation through ligand binding to the 5' mRNA coding region.
abstract: Secondary structure matters: We have constructed artificial intragenic riboswitches to probe ribosome accessibility to the 5' mRNA coding region at three-base resolution in Escherichia coli. We show that only mRNA folding stability in the +1 to +15 nt region affects the translation process.
authors.affiliation: Department of Biotechnology, Yonsei University, Shinchon-Dong 134, Seoul 120-749, South Korea.
authors.forename: Joon-Jung
authors.initials: JJ
authors.lastname: Jo
authors.forename: Joo-Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Jong-Shik
authors.initials: JS
authors.lastname: Shin
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D008024:Ligands; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010442:Peptide Chain Initiation, Translational; D059370:RNA Folding; D020871:RNA Stability; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D058928:Riboswitch
chemical_list: D052157:Aptamers, Nucleotide; D008024:Ligands; D012333:RNA, Messenger; D058928:Riboswitch