title: Effects of shock waves on oxidative stress in parotid gland of rat.
abstract: This study was designed to investigate whether extracorporeal shock wave lithotripsy (ESWL) exposure to parotid gland produces an oxidative stress in parotid glands of rats. Twelve male Wistar-albino rats, 6 months of age with an average body weight of 250-300 g, were divided randomly into two groups, each consisting of six rats. The animals in the first group did not receive any treatment and served as control. The left parotid glands of animals in group 2 (ESWL treated) received a thousand 18 kV shock waves after anesthetizing the rats with 50 mg/kg of ketamine. The animals in both groups were killed 72 hours after the ESWL treatment, and the parotid glands were harvested for the determination of lipid peroxidation product malondialdehyde (MDA), antioxidant glutathione (GSH) levels and the activities of antioxidant enzymes such as superoxide dismutase (SOD), GSH-Px and catalase (CAT). It was found that MDA level increased in parotid glands of rats after the ESWL treatment. The SOD, GSH-Px and CAT enzyme activities, and the level of antioxidant GSH decreased in parotid gland of rats after the ESWL treatment. It was concluded that short-term ESWL treatment caused an increase in the free radical production and a decrease in the antioxidant enzyme activity in parotid glands of ESWL-treated rats.
authors.affiliation: Department of Otorhinolaryngology, University of Yuzuncu Yil, Van, Turkey fatihgarca@hotmail.com.
authors.forename: M Fatih
authors.initials: MF
authors.lastname: Garca
authors.affiliation: Department of Biophysics, University of Yuzuncu Yil, Van, Turkey.
authors.forename: Servet
authors.initials: S
authors.lastname: Kavak
authors.affiliation: Department of Urology, University of Yuzuncu Yil, Van, Turkey.
authors.forename: Ilhan
authors.initials: I
authors.lastname: Gecit
authors.affiliation: Department of Physiology, Bezmialem University, İstanbul, Turkey.
authors.forename: Ismail
authors.initials: I
authors.lastname: Meral
authors.affiliation: Department of Chemistry, Division of Biochemistry, University of Yuzuncu Yil, Van, Turkey.
authors.forename: Halit
authors.initials: H
authors.lastname: Demir
authors.affiliation: Department of Otorhinolaryngology, Lokman Hekim Hospital, Van, Turkey.
authors.forename: Mahfuz
authors.initials: M
authors.lastname: Turan
authors.affiliation: Department of Anaesthesiology and Reanimation, University of Yuzuncu Yil, Van, Turkey.
authors.forename: Bilal
authors.initials: B
authors.lastname: Çeğin
authors.affiliation: Department of Biophysics, University of Yuzuncu Yil, Van, Turkey.
authors.forename: Hava
authors.initials: H
authors.lastname: Bektas
authors.affiliation: Department of Otorhinolaryngology, University of Yuzuncu Yil, Van, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Çankaya
mesh_terms: D000818:Animals; D002374:Catalase / Q000032:analysis; D005978:Glutathione / Q000032:analysis; D019220:High-Energy Shock Waves / Q000009:adverse effects*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000528:radiation effects*; D010306:Parotid Gland / Q000737:chemistry / Q000528:radiation effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione