title: Gibberellin regulates the Arabidopsis floral transition through miR156-targeted SQUAMOSA promoter binding-like transcription factors.
abstract: Gibberellin (GA), a diterpene hormone, plays diverse roles in plant growth and development, including seed germination, stem elongation, and flowering time. Although it is known that GA accelerates flowering through degradation of transcription repressors, DELLAs, the underlying mechanism is poorly understood. We show here that DELLA directly binds to microRNA156 (miR156)-targeted SQUAMOSA PROMOTER BINDING-LIKE (SPL) transcription factors, which promote flowering by activating miR172 and MADS box genes. The interaction between DELLA and SPL interferes with SPL transcriptional activity and consequently delays floral transition through inactivating miR172 in leaves and MADS box genes at shoot apex under long-day conditions or through repressing MADS box genes at the shoot apex under short-day conditions. Our results elucidate the molecular mechanism by which GA controls flowering and provide the missing link between DELLA and MADS box genes.
authors.affiliation: National Key Laboratory of Plant Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Shanghai 200032, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Yu
authors.forename: Vinicius C
authors.initials: VC
authors.lastname: Galvão
authors.forename: Yan-Chun
authors.initials: YC
authors.lastname: Zhang
authors.forename: Daniel
authors.initials: D
authors.lastname: Horrer
authors.forename: Tian-Qi
authors.initials: TQ
authors.lastname: Zhang
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Hao
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
authors.forename: Shui
authors.initials: S
authors.lastname: Wang
authors.forename: Markus
authors.initials: M
authors.lastname: Schmid
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D035264:Flowers / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005875:Gibberellins / Q000378:metabolism / Q000494:pharmacology*; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018520:Plant Shoots; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D025941:Protein Interaction Mapping; D059748:Proteolysis; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D014170:Transformation, Genetic; D020798:Two-Hybrid System Techniques
chemical_list: C419046:AGL20 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C109555:GAI protein, Arabidopsis; D005875:Gibberellins; D026161:MADS Domain Proteins; D035683:MicroRNAs; C559759:Mirn156 microRNA, Arabidopsis; C543702:Mirn172 microRNA, Arabidopsis; C508468:RGA protein, Arabidopsis; D012097:Repressor Proteins; C108465:SPL3 protein, Arabidopsis; C531404:SPL9 protein, Arabidopsis; D015534:Trans-Activators; D014157:Transcription Factors; C007842:gibberellic acid