title: Expression of metalloproteinases 1, 2, 7, 9, and 12 in human cytotrophoblastic cells from normal and preeclamptic placentas.
abstract: OBJECTIVE
  Preeclampsia is a specific pregnancy disorder which could be due, at least in part, to impaired invasion of trophoblastic cells. Since matrix metalloproteinases (MMPs) are the predominant proteases involved in trophoblastic invasion, we investigated and compared expression of MMP-1, 2, 7, 9 and 12 of cytotrophoblastic cells (CTB) purified from preeclamptic (PE) placentas to control CTB.
  
  
  METHODS
  In order to evaluate invasive properties of cells, purified CTB were seeded on collagen-coated insert following boyden chamber principle and matrix metalloproteinases (MMPs) expression was evaluated by qPCR.
  
  
  RESULTS
  Our results showed that PE CTB are less invasive than control CTB in vitro. In parallel, expression of MMPs, except for MMP-2, tends to be decreased in PE CTB compared to control CTB.
  
  
  CONCLUSIONS
  At the exception of MMP-2, this study confirms the importance of MMPs in development of PE.
authors.affiliation: Department of Gynecology Obstetrics, University of Geneva, Geneva, Switzerland. marie.cohen@hcuge.ch
authors.forename: Marie
authors.initials: M
authors.lastname: Cohen
authors.forename: Pascale
authors.initials: P
authors.lastname: Ribaux
authors.forename: Manuella
authors.initials: M
authors.lastname: Epiney
authors.forename: Olivier
authors.initials: O
authors.lastname: Irion
mesh_terms: D002478:Cells, Cultured; D005260:Female; D006801:Humans; D045726:Metalloproteases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010920:Placenta / Q000201:enzymology*; D011225:Pre-Eclampsia / Q000201:enzymology* / Q000503:physiopathology; D011247:Pregnancy; D011263:Pregnancy Trimester, Third / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D014327:Trophoblasts / Q000201:enzymology*
chemical_list: D012333:RNA, Messenger; D045726:Metalloproteases