title: Metabolic effects of intraoperative amino acid infusion in mongrel dogs.
abstract: BACKGROUND
  Intraoperative amino acid infusion can attenuate the decrease in core temperature, but the metabolic effects are uncertain.
  
  
  METHODS
  Thirty-six healthy mongrel dogs undergoing ileectomy under general anesthesia were infused intraoperatively with normal saline or 18 compound amino acids at 6, 12, and 24 kJ·kg⁻¹·h⁻¹ (NS, 6-, 12-, and 24-kJ groups) and studied until 24 h after the operation. Blood glucose, plasma insulin, free fatty acids, and triglyceride concentrations were determined at 7 defined time points. Muscle aminograms, urinary urea, and 3-methylhistidine excretions were measured before and after the operation.
  
  
  RESULTS
  Blood glucose and plasma insulin increased amino acid dose dependently during the operation and in the early period after the operation. Free fatty acids were significantly lower in the 12- and 24-kJ groups compared with the NS group at the end of the operation. The negative nitrogen balance was alleviated dose dependently in the amino acid groups on operation day. The urinary 3-methylhistidine decreased significantly during the first 24 h after the operation in the 24-kJ group, while it increased in the other groups with the largest increase in the NS group. Basic, branched-chain, and aromatic amino acids in the vastus lateralis muscle increased dose dependently at the end of the operation in the amino acid groups.
  
  
  CONCLUSIONS
  Intraoperative amino acid infusion has the dose-dependent effects of increasing blood glucose, inhibiting fat mobilization and muscle protein breakdown.
authors.affiliation: Department of Anesthesia, Zhongshan Hospital, and Department of Anesthesiology, Shanghai Medical College, Fudan University, Shanghai, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Jin
authors.forename: Shengjin
authors.initials: S
authors.lastname: Ge
authors.forename: Hongshan
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Gao
authors.forename: Jianjun
authors.initials: J
authors.lastname: Jin
authors.forename: Zhanggang
authors.initials: Z
authors.lastname: Xue
mesh_terms: D000596:Amino Acids / Q000008:administration & dosage* / Q000097:blood*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D007262:Infusions, Intravenous; D007328:Insulin / Q000097:blood; D007430:Intraoperative Care / Q000379:methods*; D008297:Male; D008762:Methylhistidines / Q000652:urine; D009124:Muscle Proteins / Q000032:analysis; D014280:Triglycerides / Q000097:blood
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D008762:Methylhistidines; D009124:Muscle Proteins; D014280:Triglycerides; C028118:3-methylhistidine