title: Bioparticles assembled using low frequency vibration immune to evacuation drifts.
abstract: The use of low frequency vibration on suspensions of glass beads in a droplet has been shown to develop a strong degree of patterning (to a ring) due to the manner with which the surface waves are modified. Functionalized glass beads that serve as bioparticles permit for sensitive readings when concentrated at specific locations. However, a time controlled exposure with analytes is desirable. The replacement of the liquid medium with analyte through extraction is needed to conserve time. Nevertheless, we show here that extraction with a porous media, which is simple and useable in the field, will strongly displace the patterned beads. The liquid removal was found to be dependent on two mechanisms that affect the shape of the droplet, one of contact hysteresis due to the outer edge pinning, and the other of liquid being drawn into the porous media. From this, we developed and demonstrated a modified well structure that prevented micro-bead displacement during evacuation. An added strong advantage with this approach lies with its ability to require only analytes to be dispensed at the location of aggregated particles, which minimizes analyte usage. This was analytically established here.
authors.affiliation: Laboratory for Optics, Acoustics and Mechanics, Monash University, Clayton, VIC3800, Australia.
authors.forename: Fenfen
authors.initials: F
authors.lastname: Shao
authors.forename: James David
authors.initials: JD
authors.lastname: Whitehill
authors.forename: Tuck Wah
authors.initials: TW
authors.lastname: Ng
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D005898:Glass / Q000737:chemistry; D008863:Microspheres; D013499:Surface Properties; D014732:Vibration