title: Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome.
abstract: Exposure to DNA-damaging agents invokes biological responses necessary for damage recovery and cell survival. Despite the presence of intact DNA repair pathways, lack of certain other biological pathways has been shown to sensitize cells to DNA-damaging agents' exposure. It is likely that following DNA damage a complex interplay between DNA repair pathways and other biological pathways might be required to ensure cell survival. In this chapter, we describe a high-throughput method for the identification of genes essential for cell survival following DNA damage by using a cell-based assay to measure viability in combination with an RNA interference-based genome-wide screening experiment.
authors.affiliation: Creehey Children's Cancer Research Institute and Department of cellular and Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
authors.forename: Dashnamoorthy
authors.initials: D
authors.lastname: Ravi
authors.forename: Alexander James Roy
authors.initials: AJ
authors.lastname: Bishop
mesh_terms: D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004249:DNA Damage; D004331:Drosophila melanogaster / Q000166:cytology* / Q000187:drug effects / Q000235:genetics*; D055785:Gene Knockdown Techniques / Q000379:methods*; D017344:Genes, Insect / Q000235:genetics*; D016133:Polymerase Chain Reaction; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D014162:Transfection
chemical_list: D012330:RNA, Double-Stranded