title: Prognostic significance of tetraspanin CD151 in newly diagnosed glioblastomas.
abstract: BACKGROUND
  Tetraspanin CD151 is a positive effector of cancer invasion and metastasis.
  
  
  METHODS
  We investigated the expression of CD151 by immunohistochemistry in 211 cases of grade I to IV gliomas. Additionally, we performed O6-methylguanin-DNA methyltransferase (MGMT) methylation analysis using real-time methylation-specific PCR in 36 patients with glioblastoma, and the prognostic significance of these biomarkers in glioblastomas was evaluated.
  
  
  RESULTS
  Overexpression of CD151 was observed in a significant proportion (55.6%) of glioblastomas, while CD151 was rarely overexpressed in most of grade I to III glial tumors. CD151 overexpression was closely associated with MGMT methylation (P = 0.014), and it was a prognostic factor for predicting worse overall survival (OS; P = 0.002) and progression-free survival (PFS; P = 0.043). We also found that combination of CD151 overexpression and MGMT methylation better stratified the patients' OS (P = 0.001) and PFS (P = 0.009). In multivariate analysis, CD151 overexpression was an independent prognostic factor for predicting OS over MGMT methylation (P = 0.012).
  
  
  CONCLUSIONS
  CD151 seems to have a critical role for high-grade progression in astroglial tumors. Furthermore, CD151 is a good tissue marker that can be used easily in a daily practice for predicting worse prognosis in patients with glioblastoma.
authors.affiliation: Department of Pathology, Kyungpook National University Hospital, Kyungpook National University School of Medicine, Daegu, Republic of Korea.
authors.forename: Dakeun
authors.initials: D
authors.lastname: Lee
authors.forename: Yeon-Lim
authors.initials: YL
authors.lastname: Suh
authors.forename: Tae-In
authors.initials: TI
authors.lastname: Park
authors.forename: In-Gu
authors.initials: IG
authors.lastname: Do
authors.forename: Ho Jun
authors.initials: HJ
authors.lastname: Seol
authors.forename: Do-Hyun
authors.initials: DH
authors.lastname: Nam
authors.forename: Sung Tae
authors.initials: ST
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001921:Brain / Q000601:surgery; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000628:therapy; D002540:Cerebral Cortex; D059186:Chemoradiotherapy, Adjuvant; D019175:DNA Methylation; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000628:therapy; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000628:therapy; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000235:genetics; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D016019:Survival Analysis; D060185:Tetraspanin 24 / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C554715:CD151 protein, human; D060185:Tetraspanin 24; D019853:O(6)-Methylguanine-DNA Methyltransferase