title: Biological response modifiers influence structure function relationship of hematopoietic stem and stromal cells in a mouse model of leukemia.
abstract: Biological response modifiers (BRMs) can alter interactions between the immune system and cancer cells to boost, direct, or restore the body's ability to fight disease. Mice with ethylnitrosourea- (ENU) induced leukemia were here used to monitor the therapeutic efficacy of lipopolysaccaride (LPS), Bacillus Calmette Guerin (BCG) and sheep erythrocytes (SRBC). Flow cytometry based CD34+ positivity analysis, clonogenicity, proliferation and ultrastructure studies using scanning electron microscopy (SEM) of stem cells in ENU induced animals with and without BRMs treatment were performed. BRMs improved the stem-stromal relationship structurally and functionally and might have potential for use as an adjunct in human stem cell therapy.
authors.affiliation: Department of Anatomy and Cell Biology, McGill University, Montreal, Canada. kaustuv.basu@mcgill.ca
authors.forename: Kaustuv
authors.initials: K
authors.lastname: Basu
authors.forename: Joydeep
authors.initials: J
authors.lastname: Mukherjee
authors.forename: Sujata
authors.initials: S
authors.lastname: Law
authors.forename: Samaresh
authors.initials: S
authors.lastname: Chaudhuri
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000032:analysis; D001500:BCG Vaccine / Q000276:immunology / Q000494:pharmacology; D049109:Cell Proliferation; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D005038:Ethylnitrosourea; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D007155:Immunologic Factors / Q000494:pharmacology*; D007942:Leukemia, Experimental / Q000276:immunology* / Q000473:pathology* / Q000628:therapy; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D012756:Sheep; D017154:Stromal Cells / Q000187:drug effects / Q000276:immunology / Q000502:physiology*
chemical_list: D018952:Antigens, CD34; D001500:BCG Vaccine; D007155:Immunologic Factors; D008070:Lipopolysaccharides; D005038:Ethylnitrosourea