title: A chemical approach to mapping nucleosomes at base pair resolution in yeast.
abstract: Most eukaryotic DNA exists in DNA-protein complexes known as nucleosomes. The exact locations of nucleosomes along the genome play a critical role in chromosome functions and gene regulation. However, the current methods for nucleosome mapping do not provide the necessary accuracy to identify the precise nucleosome locations. Here we describe a new experimental approach that directly maps nucleosome center locations in vivo genome-wide at single base pair resolution.
authors.affiliation: Department of Molecular Biosciences, Northwestern University, Evanston, Illinois, USA. Kristin.Brogaard@systemsbiology.org
authors.forename: Kristin R
authors.initials: KR
authors.lastname: Brogaard
authors.forename: Liqun
authors.initials: L
authors.lastname: Xi
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Wang
authors.forename: Jonathan
authors.initials: J
authors.lastname: Widom
mesh_terms: D001482:Base Composition; D020029:Base Pairing; D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal / Q000737:chemistry* / Q000235:genetics; D019295:Computational Biology / Q000379:methods; D003545:Cysteine / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics; D016681:Genome, Fungal; D006657:Histones / Q000737:chemistry / Q000235:genetics; D016296:Mutagenesis; D009707:Nucleosomes / Q000737:chemistry* / Q000235:genetics; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D012694:Serine / Q000737:chemistry; D014157:Transcription Factors
chemical_list: D004271:DNA, Fungal; D006657:Histones; D009707:Nucleosomes; D014157:Transcription Factors; D012694:Serine; D003545:Cysteine