title: Anti-inflammatory effect of sargachromanol G isolated from Sargassum siliquastrum in RAW 264.7 cells.
abstract: A study on the anti-inflammatory activity of brown alga Sargassum siliquastrum led to the isolation of sargachromanol G (SG). In this study, the anti-inflammatory effect and the action mechanism of SG have been investigated in murine macrophage cell line RAW 264.7. SG dosedependently inhibited the production of inflammatory markers [nitric oxide (NO), inducible nitric oxide synthase (iNOS), prostaglandin E(2) (PGE(2)), and cyclooxygenase-2 (COX-2)] and pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6] induced by LPS treatment. To further elucidate the mechanism of this inhibitory effect of SG, we studied LPS-induced nuclear factor-κB (NF-κB) activation and mitogen-activated protein kinases (MAPKs) phosphorylation. SG inhibited the phosphorylation IκB-α and NF-κB (p65 and p50) and MAPK (ERK1/2, JNK, and p38) in a dose dependent manner. These results suggest that the anti-inflammatory activity of SG results from its modulation of pro-inflammatory cytokines and mediators via the suppression of NF-κB activation and MAPK phosphorylation.
authors.affiliation: School of Medicine, Institute of Medical Science, Jeju National University, Jeju, 690-756, Korea.
authors.forename: Weon-Jong
authors.initials: WJ
authors.lastname: Yoon
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Heo
authors.forename: Sang-Chul
authors.initials: SC
authors.lastname: Han
authors.forename: Hye-Ja
authors.initials: HJ
authors.lastname: Lee
authors.forename: Gyeoung-Jin
authors.initials: GJ
authors.lastname: Kang
authors.forename: Hee-Kyoung
authors.initials: HK
authors.lastname: Kang
authors.forename: Jin-Won
authors.initials: JW
authors.lastname: Hyun
authors.forename: Young-Sang
authors.initials: YS
authors.lastname: Koh
authors.forename: Eun-Sook
authors.initials: ES
authors.lastname: Yoo
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D001578:Benzopyrans / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D044647:Sargassum / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D001578:Benzopyrans; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D016328:NF-kappa B; C577249:sargachromanol G; D020928:Mitogen-Activated Protein Kinases