title: Association of genetic variants on 8p21 and 4q12 with age-related macular degeneration in Asian populations.
abstract: OBJECTIVE
  To evaluate the association of genetic variants at chromosomes 8p21 and 4q12 with the risk of developing AMD and its two main subtypes, choroidal neovascular membrane (CNV) and polypoidal choroidal vasculopathy (PCV), in Asian populations.
  
  
  METHODS
  The study population comprised 2360 patients with neovascular AMD (1013 typical AMD-CNV and 1282 PCV), and 3598 controls from four independent cohorts, two of Japanese (n = 4859) and two of Chinese (n = 1099) ethnicity. We performed a meta-analysis in case-control studies of two reported single nucleotide polymorphisms (SNPs) (rs13278062 at TNFRSF10A-LOC389641 on 8p21 and rs1713985 at REST-C4orf14-POLR2B-IGFBP7 on 4q12) by using logistic regression analysis adjusted for age and sex. Subgroup analysis by CNV and PCV subtypes were performed to evaluate the significance of these two variants.
  
  
  RESULTS
  The reported association between rs13278062 at 8p21 and neovascular AMD was replicated in this population (P = 1.12 × 10(-4), odds ratio [OR] = 0.79, 95% confidence interval [CI] = 0.70-0.89). However, there was no association of rs1713985 at 4q12 with neovascular AMD, or its two subtypes, typical AMD-CNV and PCV (all P > 0.05). The study sample size had a statistical power of greater than 99% to detect an association of a risk allele with AMD with an OR of 1.30, as reported in the original study of rs1713985 and AMD.
  
  
  CONCLUSIONS
  The present results did not replicate the reported association between rs1713985 at 4q12 and neovascular AMD. However, we confirmed the association between rs13278062 at 8p21 and neovascular AMD in Asian populations.
authors.affiliation: Department of Ophthalmology and the Center for Genomic Medicine/INSERM U.852, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Nakata
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamashiro
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Akagi-Kurashige
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyake
authors.forename: Kyoko
authors.initials: K
authors.lastname: Kumagai
authors.forename: Akitaka
authors.initials: A
authors.lastname: Tsujikawa
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Li Jia
authors.initials: LJ
authors.lastname: Chen
authors.forename: David T L
authors.initials: DT
authors.lastname: Liu
authors.forename: Timothy Y Y
authors.initials: TY
authors.lastname: Lai
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Sakurada
authors.forename: Seigo
authors.initials: S
authors.lastname: Yoneyama
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Cheng
authors.forename: Peter
authors.initials: P
authors.lastname: Cackett
authors.forename: Ian Y
authors.initials: IY
authors.lastname: Yeo
authors.forename: Wan Ting
authors.initials: WT
authors.lastname: Tay
authors.forename: Belinda K
authors.initials: BK
authors.lastname: Cornes
authors.forename: Eranga N
authors.initials: EN
authors.lastname: Vithana
authors.forename: Tin
authors.initials: T
authors.lastname: Aung
authors.forename: Keitaro
authors.initials: K
authors.lastname: Matsuo
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Matsuda
authors.forename: Tien-Yin
authors.initials: TY
authors.lastname: Wong
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iijima
authors.forename: Chi Pui
authors.initials: CP
authors.lastname: Pang
authors.forename: Nagahisa
authors.initials: N
authors.lastname: Yoshimura
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D015862:Choroid Diseases / Q000235:genetics; D020256:Choroidal Neovascularization / Q000235:genetics; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008268:Macular Degeneration / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011127:Polyps / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors