title: [Expressions and clinical significance of high mobility group box-1 mRNA and protein in laryngeal squamous cell carcinoma tissues and serum].
abstract: OBJECTIVE
  To investigate the mRNA and protein expressions of high mobility group box-1 (HMGB1) in the tumor tissues and sera of patients with laryngeal squamous cell carcinoma (LSCC) and their clinical significance.
  
  
  METHODS
  Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were used to detected the expressions of HMGB1 mRNA and protein in the tumors and adjacent normal epithelial tissues in 30 patients with LSCC. Serum HMGB1 protein levels in the patients with LSCC and in 10 healthy volunteers were detected with enzyme-linked immunosorbent adsorption experiment (ELISA).
  
  
  RESULTS
  RT-PCR demonstrated that the mean relative mRNA expression levels of HMGB1 (HMGB1/GAPDH) in LSCC tissues and in adjacent normal epithelial tissues were 1.25 ± 0.12 and 0.32 ± 0.04, respectively (t = 40.27, P < 0.05). Western blot revealed that the mean relative protein expression levels of HMGB1 (HMGB1/β-actin) were 1.29 ± 0.10 and 0.34 ± 0.03 (t = 49.84, P < 0.05), respectively. Both mRNA and protein expression levels of HMGB1 were associated with T stage, clinical stage, lymph node metastasis status and smoking (all P < 0.05), but no significant correlation with age, alcohol consumption and primary tumor grade and location (all P > 0.05). Mean serum HMGB1 protein levels in patients with LSCC and healthy volunteers were (24.80 ± 14.08) ng/ml and (23.58 ± 14.69) ng/ml (t = 0.37, P > 0.05).
  
  
  CONCLUSIONS
  Both mRNA and protein expressions of HMGB1 were obviously elevated in LSCC, which were associated closely with T stage, clinical stage and lymph node metastasis.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Ping-qing
authors.initials: PQ
authors.lastname: Tan
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.forename: Zhong-wu
authors.initials: ZW
authors.lastname: Su
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Tian
authors.forename: Yuan-zheng
authors.initials: YZ
authors.lastname: Qiu
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D024243:HMGB1 Protein / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000097:blood / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics / Q000378:metabolism
chemical_list: D024243:HMGB1 Protein; D012333:RNA, Messenger