title: Post-translational modification of the RhoGTPase activating protein 21, ARHGAP21, by SUMO2/3.
abstract: ARHGAP21 is a 217 kDa RhoGAP protein shown to modulate cell migration through the control of Cdc42 and FAK activities. In the present work a 250 kDa-ARHGAP21 was identified by mass spectrometry. This modified form is differentially expressed among cell lines and human primary cells. Co-immunoprecipitations and in vitro SUMOylation confirmed ARHGAP21 specific modification by SUMO2/3 and mapped the SUMOylation site to ARHGAP21 lysine K1443. Immunofluorescence staining revealed that ARHGAP21 co-localizes with SUMO2/3 in the cytoplasm and membrane compartments. Interestingly, our results suggest that ARHGAP21 SUMOylation may be related to cell proliferation. Therefore, SUMOylation of ARHGAP21 may represent a way of guiding its function.
authors.affiliation: Hematology and Hemotherapy Center, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Carolina L
authors.initials: CL
authors.lastname: Bigarella
authors.forename: Karla P Vieira
authors.initials: KP
authors.lastname: Ferro
authors.forename: Karin S A
authors.initials: KS
authors.lastname: Barcellos
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
authors.forename: Fabiola
authors.initials: F
authors.lastname: Traina
authors.forename: José C
authors.initials: JC
authors.lastname: Novello
authors.forename: Sara T Olalla
authors.initials: ST
authors.lastname: Saad
authors.forename: Leticia Fröhlich
authors.initials: LF
authors.lastname: Archangelo
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020690:GTPase-Activating Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008239:Lysine / Q000737:chemistry; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D058207:Sumoylation; D014452:Ubiquitins / Q000378:metabolism*
chemical_list: C459590:ARHGAP21 protein, human; D020690:GTPase-Activating Proteins; D011993:Recombinant Fusion Proteins; C117909:SUMO2 protein, human; C492189:SUMO3 protein, human; D025841:Small Ubiquitin-Related Modifier Proteins; D014452:Ubiquitins; D008239:Lysine