title: DNA repair gene expression in biological tissues exposed to low-intensity infrared laser.
abstract: Special properties of laser light have led to its usefulness in many applications in therapy. Excitation of endogenous chromophores in biotissues and generation of free radicals could be involved in its biological effects. DNA lesions induced by free radicals are repaired by base excision repair pathway. In this work, we evaluated the expression of APE1 and OGG1 genes related to repair of DNA lesions induced by free radicals. Skin and muscle tissues of Wistar rats were exposed to low-intensity infrared laser at different fluences and frequencies. After laser exposition of 1 and 24 h, tissue samples were withdrawn for total RNA extraction, cDNA synthesis, and evaluation of APE1 and OGG1 gene expression by quantitative polymerase chain reaction. Data obtained show that laser radiation alters the expression of APE1 and OGG1 mRNA differently in skin and muscle tissues of Wistar rats depending of the fluence, frequency, and time after exposure. Our study suggests that low-intensity infrared laser affects expression of genes involved in repair of DNA lesions by base excision repair pathway.
authors.affiliation: Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, Maracanã, Rio de Janeiro 20550900, Brazil. adnfonseca@ig.com.br
authors.forename: Adenilson
authors.initials: A
authors.lastname: de Souza da Fonseca
authors.forename: Andre Luiz
authors.initials: AL
authors.lastname: Mencalha
authors.forename: Vera Maria
authors.initials: VM
authors.lastname: Araújo de Campos
authors.forename: Samara Cristina
authors.initials: SC
authors.lastname: Ferreira Machado
authors.forename: Antonio Augusto
authors.initials: AA
authors.lastname: de Freitas Peregrino
authors.forename: Mauro
authors.initials: M
authors.lastname: Geller
authors.forename: Flavia
authors.initials: F
authors.lastname: de Paoli
mesh_terms: D000818:Animals; D045647:DNA Glycosylases / Q000235:genetics; D004260:DNA Repair / Q000235:genetics* / Q000528:radiation effects*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics; D015870:Gene Expression / Q000528:radiation effects; D007259:Infrared Rays / Q000627:therapeutic use; D028022:Low-Level Light Therapy; D008297:Male; D009132:Muscles / Q000378:metabolism / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000378:metabolism / Q000528:radiation effects
chemical_list: D012333:RNA, Messenger; D045647:DNA Glycosylases; C483741:OGG1 protein, rat; C470319:Apex1 protein, rat; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase