title: Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection.
abstract: The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated. The findings show that especially the propensity for mismatch self-dimer formation of the DNA probes can be a good indicator for the creation and stabilization of red emissive AgNCs. Also, the role of the thermal stability of the secondary DNA structures (mismatch self-dimer and hairpin monomers) and the observed AgNC red emission intensity were investigated. These findings can form the basis for a rationale to design new red emissive AgNC-based probes. As an example, a bright red emissive AgNC-based DNA probe was designed for RNA-miR172 detection. The latter opens the possibility to create a variety of AgNC-based DNA probes for the specific detection of plant and animal miRNAs.
authors.affiliation: Department of Plant Biology and Biotechnology, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg, Copenhagen, Denmark.
authors.forename: Pratik
authors.initials: P
authors.lastname: Shah
authors.forename: Andreas
authors.initials: A
authors.lastname: Rørvig-Lund
authors.forename: Samir Ben
authors.initials: SB
authors.lastname: Chaabane
authors.forename: Peter Waaben
authors.initials: PW
authors.lastname: Thulstrup
authors.forename: Henrik Grum
authors.initials: HG
authors.lastname: Kjaergaard
authors.forename: Eduard
authors.initials: E
authors.lastname: Fron
authors.forename: Johan
authors.initials: J
authors.lastname: Hofkens
authors.forename: Seong Wook
authors.initials: SW
authors.lastname: Yang
authors.forename: Tom
authors.initials: T
authors.lastname: Vosch
mesh_terms: D015342:DNA Probes / Q000032:analysis* / Q000235:genetics*; D053768:Metal Nanoparticles / Q000032:analysis / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D015336:Molecular Probe Techniques; D012834:Silver / Q000032:analysis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D015342:DNA Probes; D035683:MicroRNAs; D012834:Silver