title: Hybrid nanomaterial for stabilizing the antibiofilm activity of Eugenia carryophyllata essential oil.
abstract: The aim of the present study was to demonstrate that   Fe(3)O(4)/oleic acid  core/shell nanostructures could be used as systems for stabilizing the Eugenia carryophyllata essential oil (EO) on catheter surface pellicles, in order to improve their resistance to fungal colonization. EO microwave assisted extraction was performed in a Neo-Clevenger (related) device and its chemical composition was settled by GC-MS analysis.  Fe(3)O(4)/oleic acid-core/shell nanoparticles (NP) were obtained by a precipitation method under microwave condition. High resolution transmission electron microscopy (HR-TEM) was used as a primary characterization method. The NPs were processed to achieve a core/shell/EO coated-shell nanosystem further used for coating the inner surface of central venous catheter samples. The tested fungal strains have been recently isolated from different clinical specimens. The biofilm architecture was assessed by confocal laser scanning microscopy (CLSM). Our results claim the usage of hybrid nanomaterial (core/shell/coated-shell) for the stabilization of E. carryophyllata EO, which prevented or inhibited the fungal biofilm development on the functionalized catheter, highlighting the opportunity of using these nanosystems to obtain improved, anti-biofilm coatings for biomedical applications.
authors.affiliation: Department of Science and Engineering of Oxidic Materials and Nanomaterials, University Politehnica of Bucharest, Bucharest, 011061, Romania. grumezescu@yahoo.com
authors.forename: Alexandru Mihai
authors.initials: AM
authors.lastname: Grumezescu
authors.forename: Mariana Carmen
authors.initials: MC
authors.lastname: Chifiriuc
authors.forename: Crina
authors.initials: C
authors.lastname: Saviuc
authors.forename: Valentina
authors.initials: V
authors.lastname: Grumezescu
authors.forename: Radu
authors.initials: R
authors.lastname: Hristu
authors.forename: Dan Eduard
authors.initials: DE
authors.lastname: Mihaiescu
authors.forename: George A
authors.initials: GA
authors.lastname: Stanciu
authors.forename: Ecaterina
authors.initials: E
authors.lastname: Andronescu
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002175:Candida / Q000187:drug effects / Q000502:physiology; D058185:Magnetite Nanoparticles / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D019301:Oleic Acid / Q000737:chemistry / Q000494:pharmacology*; D027842:Syzygium; D062666:Vascular Access Devices
chemical_list: D000935:Antifungal Agents; D058185:Magnetite Nanoparticles; D009822:Oils, Volatile; D019301:Oleic Acid