title: Isolation and identification of fourteen microsatellite markers in Clivia miniata and Clivia nobilis (Amaryllidaceae).
abstract: Clivia is a genus of great horticultural importance and has been widely cultivated as ornamental plants in all over the world. In order to assess the phylogenetic relationships and genetic diversity of the wild Clivia species and cultivars, we isolated AC-enriched repeats using FIASCO from a single clone each of C. miniata Regel. and Clivia nobilis Lindl. Of the fourteen repeats, 10 were polymorphic and 4 were monomorphic. The polymorphic marker loci were characterized using 61 Clivia accessions. The number of alleles ranged from two to six, observed heterozygosity ranged from 0.04 to 1.00 and expected heterozygosity ranged from 0.04 to 0.83. These microsatellite marker loci provide tools for future studies of Clivia species and cultivars.
authors.affiliation: College of Traditional Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China.|College of Biological Science and Technology, Changchun University, Changchun 130012, China.|Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.affiliation: Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China.
authors.forename: He
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China.
authors.forename: Ming-Rui
authors.initials: MR
authors.lastname: Li
authors.affiliation: College of Chemistry and Life Sciences, Yili Normal University, Yili, 835000, China.
authors.forename: Eliyas
authors.initials: E
authors.lastname: Nurmamat
authors.affiliation: Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China.
authors.forename: Lin-Feng
authors.initials: LF
authors.lastname: Li
authors.affiliation: College of Traditional Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China.
authors.forename: Yue-Ying
authors.initials: YY
authors.lastname: Ren
authors.affiliation: Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun 130024, China.
authors.forename: Hong-Xing
authors.initials: HX
authors.lastname: Xiao
mesh_terms: D019668:Liliaceae / Q000145:classification* / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D013045:Species Specificity