title: Determination of L-trantinterol in rat plasma by using chiral liquid chromatography-tandem mass spectrometry.
abstract: A simple, sensitive, and rapid method for determination of L-trantinterol in rat plasma was developed for the first time by using LC coupled to MS/MS based on chiral stationary phase. A baseline separation of the enantiomers of trantinterol was achieved on a Chirobiotic V column, using a mixture of acetonitrile-methanol-ammonia-acetic acid (80:20:0.01:0.02, v/v/v/v) as the mobile phase. The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring mode via ESI. The calibration curve was linear in concentration range from 0.270 to 108 ng/mL in plasma with the lower limit of quantification of 0.270 ng/mL. The intra- and interday precision (relative standard deviation) values were within 10.9% and the accuracy (relative error) was from 2.6 to 9.2% at all quality control levels. The method has been successfully applied to a study of L-trantinterol pharmacokinetics in rats.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Jing
authors.forename: Kunjie
authors.initials: K
authors.lastname: Li
authors.forename: Fen
authors.initials: F
authors.lastname: Qin
authors.forename: Xintao
authors.initials: X
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.forename: Yanjuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Maosheng
authors.initials: M
authors.lastname: Cheng
authors.forename: Famei
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002976:Clenbuterol / Q000031:analogs & derivatives* / Q000097:blood; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: C542064:trantinterol; D002976:Clenbuterol