title: Mitochondrial genome of Angiostrongylus vasorum: comparison with congeners and implications for studying the population genetics and epidemiology of this parasite.
abstract: Angiostrongylus vasorum is a strongylid nematode of major clinical relevance in canids, causing angiostrongylosis. In spite of its increasing importance, the genetics, epidemiology and biology of this parasite are not entirely understood. Mitochondrial (mt) DNA provides useful markers for studies of these areas, but genetic data are scant for A. vasorum and its congeners. Here, the mitochondrial genome was amplified by long-range polymerase chain reaction (long-PCR) from a portion of a single male adult of A. vasorum, sequenced using 454 technology and annotated employing a semi-automated bioinformatic pipeline. This circular mitochondrial genome is 13,422 bp and contains 12 protein-encoding, 22 transfer RNA, and two ribosomal RNA genes, consistent with its congeners and other secernentean nematodes. This mt genome represents a rich source of markers for future investigations of the population genetics and epidemiology of A. vasorum. Molecular tools, employing such mt markers, should be useful for explorations into host specificity and for prospecting for cryptic species, and might also underpin the diagnosis of canine angiostrongylosis.
authors.affiliation: Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia. robinbg@unimelb.edu.au
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
authors.forename: Abdul
authors.initials: A
authors.lastname: Jabbar
authors.forename: Namitha
authors.initials: N
authors.lastname: Mohandas
authors.forename: Manuela
authors.initials: M
authors.lastname: Schnyder
authors.forename: Peter
authors.initials: P
authors.lastname: Deplazes
authors.forename: D Timothy J
authors.initials: DT
authors.lastname: Littlewood
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Jex
mesh_terms: D000801:Angiostrongylus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D003062:Codon; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D005260:Female; D050259:Genes, Mitochondrial; D005819:Genetic Markers / Q000235:genetics; D049751:Genome, Helminth; D054629:Genome, Mitochondrial; D015801:Helminth Proteins / Q000235:genetics; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017206:Strongylida Infections / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D003062:Codon; D005819:Genetic Markers; D015801:Helminth Proteins