title: Inhibition on hepatitis B virus in vitro of lectin from Musca domestica pupa via the activation of NF-κB.
abstract: The present study reported that the secretions of HBsAg and HBeAg in HepG2.2.15 cells were significantly decreased under the treatment of lectin from Musca domestica pupa (MPL). Both the replication of hepatitis B virus (HBV) DNA and HBV cccDNA in cells, and the copies of extracellular HBV DNA were inhibited by MPL. The mRNA expressions of interleukin-2 (IL-2), gamma interferon (INF-γ) and MxA were up-regulated by MPL treatments, but down-regulated when nuclear factor-κB (NF-κB) signal pathway was blocked by pyrrolidine dithiocarbamate (PDTC). Subsequent investigation revealed that nuclear factor-κB inhibitory κB (IκB) in endochylema was inhibited and NF-κB was translocated into the nucleus. These findings indicate that MPL could inhibit HBV replication via the induction of the expression of IL-2, INF-γ and MxA through the activation of NF-κB.
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Avenue, Tianjin Economy Technological Development Area, Tianjin 300457, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Cao
authors.forename: Minghui
authors.initials: M
authors.lastname: Zhou
authors.forename: Songxue
authors.initials: S
authors.lastname: Wang
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
authors.forename: Lihua
authors.initials: L
authors.lastname: Hou
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Luo
authors.forename: Linye
authors.initials: L
authors.lastname: Chen
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D004261:DNA Replication / Q000187:drug effects; D004270:DNA, Circular; D004279:DNA, Viral; D004789:Enzyme Activation; D005786:Gene Expression Regulation / Q000187:drug effects; D006515:Hepatitis B virus / Q000187:drug effects* / Q000502:physiology; D006793:Houseflies / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000494:pharmacology* / Q000633:toxicity; D008954:Models, Biological; D016328:NF-kappa B / Q000378:metabolism*; D011679:Pupa / Q000737:chemistry*; D015398:Signal Transduction / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000956:Antigens, Viral; D016207:Cytokines; D004270:DNA, Circular; D004279:DNA, Viral; D037102:Lectins; D016328:NF-kappa B