title: [Methylation status of P16 gene during malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate].
abstract: OBJECTIVE
  To analyze the methylation status of P16 gene at the different stages of malignant transformation of human bronchial epithelial cells (16HBE) induced by glycidyl methacrylate (GMA) and to explore the DNA methylation mechanisms.
  
  
  METHODS
  The cells exposed to GMA were harvested at the end of exposure (early stage), the 10th generation (protophase) and the 30th generation (anaphase), respectively. The methylation status of P16 promotor was detected by Methylation-specific PCR (MSP). The transformed 16HBE cells were compared with the normal 16HBE cells and the cells exposed to DMSO for methylation status.
  
  
  RESULTS
  At the early stage and protophase stage, the non-methylation status in P16 gene promotor of the normal 16HBE cells and the cells exposed to DMSO appeared, the methylation status in P16 gene promotor of the 16HBE cells exposed to GMA was detected to some extension. At the anaphase stage, the methylation status in P16 gene promotor of the 16HBE cells exposed to GMA or DMSO was detected to some extension.
  
  
  CONCLUSIONS
  Methylation status of P16 gene promoter was specific at the early stage and protophase stage of malignant transforming in 16HBE cells induced by GMA, which can serve as an early sensitive biological indicator for malignant transforming in 16HBE cells induced by GMA.
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Hu
authors.forename: Quan-kai
authors.initials: QK
authors.lastname: Wang
authors.forename: An-na
authors.initials: AN
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Dong
authors.forename: Jian-ning
authors.initials: JN
authors.lastname: Xu
mesh_terms: D001980:Bronchi / Q000166:cytology / Q000187:drug effects / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002478:Cells, Cultured; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation; D004847:Epithelial Cells / Q000187:drug effects / Q000473:pathology*; D004852:Epoxy Compounds / Q000633:toxicity*; D006801:Humans; D008689:Methacrylates / Q000633:toxicity*; D011401:Promoter Regions, Genetic
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D004852:Epoxy Compounds; D008689:Methacrylates; C007870:glycidyl methacrylate