title: Identification of a novel B-cell epitope of Hantaan virus glycoprotein recognized by neutralizing 3D8 monoclonal antibody.
abstract: Hantaan virus (HTNV), a member of the family Bunyaviridae, is a major agent causing haemorrhagic fever with renal syndrome, a high-mortality-rate disease threatening approximately 150 000 people around the world yearly. The 3D8 mAb displays a neutralizing activity to HTNV infection. In this study, the B-cell epitopes of HTNV glycoproteins (GPs) were finely mapped by peptide scanning. A new B-cell epitope (882)GFLCPEFPGSFRKKC(896) of HTNV, which locates on Gc, has been screened out from a set of 15-mer synthesized peptides covering the full-length of HTNV-GPs. It has been shown by the alanine-scanning technique that (885)C, (893)R, (894)K, (895)K and (896)C are the key amino acids of the binding sites of the GPs. The implications of identifying a novel B-cell epitope for hantavirus immunology and vaccinology are discussed.
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Yan
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Yusi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yi
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Bei
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Zhuwei
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Fanglin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Zhikai
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Angang
authors.initials: A
authors.lastname: Yang
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhuang
authors.affiliation: Department of Immunology, the Fourth Military Medical University, 17 Changle West Road, Xi'an, Shaanxi 710032, PR China.
authors.forename: Boquan
authors.initials: B
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D001665:Binding Sites / Q000235:genetics; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology*; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D018063:Hantaan virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D018985:Epitopes, B-Lymphocyte; D006023:Glycoproteins; D014764:Viral Proteins