title: High performance liquid chromatography used for quality control of Achyranthis Radix.
abstract: To establish a standard of quality control and to identify reliable Achyranthis Radix, three phytoecdysones including ecdysterone (1), 25R-inokosterone (2) and 25S-inokosterone (3) were determined by quantitative HPLC/UV analysis. Three phytoecdysones were separated with an YMC J'sphere ODS C(18) column (250 mm × 4.6 mm, 4 μm) by isocratic elution using 0.1% formic acid in water and acetonitrile (85:15, v/v%) as the mobile phase. The flow rate was 1.0 mL/min and the UV detector wavelength was set at 245 nm. The standards were quantified by HPLC/UV from Achyranthes bidentata Blume and Achyranthes japonica Nakai, as well as Cyathula capitata Moq. and Cyathula officinalis Kuan, which are of a different genus but are comparative herbs. The method was successfully used in the analysis of Achyranthis Radix of different geographical origin or genera with relatively simple conditions and procedures, and the assay results were satisfactory for linearity, recovery, precision, accuracy, stability and robustness. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of eighteen A. bidentata Blume samples and ten A. japonica Nakai samples. The results indicate that the established HPLC/UV method is suitable for quantitation and pattern recognition analyses for quality evaluation of Achyranthis Radix.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, Korea.
authors.forename: Bing Tian
authors.initials: BT
authors.lastname: Zhao
authors.forename: Su Yang
authors.initials: SY
authors.lastname: Jeong
authors.forename: Dong Cheul
authors.initials: DC
authors.lastname: Moon
authors.forename: Kun Ho
authors.initials: KH
authors.lastname: Son
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Woo
mesh_terms: D029044:Achyranthes / Q000737:chemistry*; D002782:Cholestenes / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004440:Ecdysone / Q000032:analysis* / Q000302:isolation & purification; D011786:Quality Control; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013237:Stereoisomerism
chemical_list: D002782:Cholestenes; C100084:inokosterone; D004440:Ecdysone