title: [Effect of hydrogen inhalation on p38 MAPK activation in rats with lipopolysaccharide- induced acute lung injury].
abstract: OBJECTIVE
  To investigate the effect of hydrogen inhalation on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the underlying molecular mechanisms.
  
  
  METHODS
  Thirty-two male SD rats were randomly divided into 4 groups (n=8), namely the normal saline group (SA), saline with 2% hydrogen gas inhalation group (SH group), ALI group, and ALI with hydrogen inhalation group (LH group). In the two ALI groups, ALI was induced by intraperitoneal injection of 15 mg/kg LPS. Treatments with inhalation of 2% hydrogen gas for 6 h was administered after the injection of LPS or saline. The concentrations of tumor necrosis factor-α (TNF-α) in the lung tissue and serum were examined with ELISA. The expression of p38 MAPK in the lung tissue was detected by Western blotting..
  
  
  RESULTS
  Hydrogen inhalation decreased the expression of p-p38 MAPK in the lung tissue, and significantly reduced TNF-α content in the lung tissue and serum of rats with ALI.
  
  
  CONCLUSIONS
  Hydrogen inhalation can decrease the expression of TNF-α in the lung tissue and serum, and this effect may be related with reduced p38 MAPK expression and inhibition of p38 MAPK activation.
authors.affiliation: Department of Anesthesiology, First Affiliated Hospital of Chongqing Medical University, Chongqing, China. 76353724@qq.com
authors.forename: Canxin
authors.initials: C
authors.lastname: Liang
authors.forename: Xinwei
authors.initials: X
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Dongwei
authors.initials: D
authors.lastname: He
mesh_terms: D055371:Acute Lung Injury / Q000097:blood / Q000139:chemically induced / Q000378:metabolism*; D000280:Administration, Inhalation; D000818:Animals; D006859:Hydrogen / Q000008:administration & dosage*; D008070:Lipopolysaccharides; D008168:Lung / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000097:blood / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D006859:Hydrogen; D048051:p38 Mitogen-Activated Protein Kinases