title: Geographical distribution updating of Tunisian leishmaniasis foci: about the isoenzymatic analysis of 694 strains.
abstract: Over a period of ten years, a series of 694 Leishmania strains from Tunisian leishmaniasis foci were isolated and identified by isoenzymatic analysis. Strains were obtained from human cutaneous and visceral leishmaniasis in immunocompetent subjects, visceral leishmaniasis in imunocompromised individuals and from dogs with visceral leishmaniasis. Two classically dermotropic species, Leishmania (L.) major and Leishmania killicki were found. L. major with the single zymodeme MON-25 was the most isolated in cutaneous leishmaniasis foci of the Centre and South of Tunisia with a recent northern extension. L. killicki zymodeme MON-8 was sporadically found both in its classical microfocus of Tataouine in southeastern Tunisia as well as in some new foci in Southwestern, Central and Northern Tunisia. Leishmania infantum with its three zymodemes MON-1, MON-24 and MON-80 was isolated from both visceral and cutaneous human cases. The majority of L. infantum strains were found in the Northern part of the country; however, some strains were reported for the first time in the Southern part. L. infantum MON-1 was the only zymodeme isolated from canine leishmaniasis.
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Faculté de Pharmacie, Département de Biologie Clinique B, Monastir, Tunisia.
authors.forename: N
authors.initials: N
authors.lastname: Haouas
authors.forename: E
authors.initials: E
authors.lastname: Chaker
authors.forename: N
authors.initials: N
authors.lastname: Chargui
authors.forename: M
authors.initials: M
authors.lastname: Gorcii
authors.forename: S
authors.initials: S
authors.lastname: Belhadj
authors.forename: K
authors.initials: K
authors.lastname: Kallel
authors.forename: K
authors.initials: K
authors.lastname: Aoun
authors.forename: F Messaadi
authors.initials: FM
authors.lastname: Akrout
authors.forename: M
authors.initials: M
authors.lastname: Ben Said
authors.forename: F
authors.initials: F
authors.lastname: Pratlong
authors.forename: J-P
authors.initials: JP
authors.lastname: Dedet
authors.forename: H
authors.initials: H
authors.lastname: Mezhoud
authors.forename: P
authors.initials: P
authors.lastname: Lami
authors.forename: M
authors.initials: M
authors.lastname: Zribi
authors.forename: R
authors.initials: R
authors.lastname: Azaiez
authors.forename: H
authors.initials: H
authors.lastname: Babba
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D018314:Leishmania infantum / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D018320:Leishmania major / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D007896:Leishmaniasis / Q000453:epidemiology* / Q000469:parasitology / Q000662:veterinary*; D017720:Molecular Epidemiology; D019371:Topography, Medical; D014416:Tunisia / Q000453:epidemiology
chemical_list: D007527:Isoenzymes