title: Autoreceptor control of peptide/neurotransmitter corelease from PDF neurons determines allocation of circadian activity in drosophila.
abstract: Drosophila melanogaster flies concentrate behavioral activity around dawn and dusk. This organization of daily activity is controlled by central circadian clock neurons, including the lateral-ventral pacemaker neurons (LN(v)s) that secrete the neuropeptide PDF (pigment dispersing factor). Previous studies have demonstrated the requirement for PDF signaling to PDF receptor (PDFR)-expressing dorsal clock neurons in organizing circadian activity. Although LN(v)s also express functional PDFR, the role of these autoreceptors has remained enigmatic. Here, we show that (1) PDFR activation in LN(v)s shifts the balance of circadian activity from evening to morning, similar to behavioral responses to summer-like environmental conditions, and (2) this shift is mediated by stimulation of the Gα,s-cAMP pathway and a consequent change in PDF/neurotransmitter corelease from the LN(v)s. These results suggest another mechanism for environmental control of the allocation of circadian activity and provide new general insight into the role of neuropeptide autoreceptors in behavioral control circuits.
authors.affiliation: Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, CT 06520, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Choi
authors.forename: Guan
authors.initials: G
authors.lastname: Cao
authors.forename: Anne K
authors.initials: AK
authors.lastname: Tanenhaus
authors.forename: Ellena V
authors.initials: EV
authors.lastname: McCarthy
authors.forename: Misun
authors.initials: M
authors.lastname: Jung
authors.forename: William
authors.initials: W
authors.lastname: Schleyer
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Shang
authors.forename: Michael
authors.initials: M
authors.lastname: Rosbash
authors.forename: Jerry C P
authors.initials: JC
authors.lastname: Yin
authors.forename: Michael N
authors.initials: MN
authors.lastname: Nitabach
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D002940:Circadian Rhythm / Q000502:physiology*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D007447:Invertebrate Hormones / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D029721:Drosophila Proteins; D007447:Invertebrate Hormones; C507073:PDFR protein, Drosophila; D011498:Protein Precursors; D043562:Receptors, G-Protein-Coupled; C079085:pigment dispersing hormone precursor