title: [Biological effect of micro ribonucleic acid-21 on human tongue squamous cell carcinoma lines].
abstract: OBJECTIVE
  To detect the biological influence to human tongue squamous cell carcinoma (TSCC) cells of micro ribonucleic acid-21 (miR-21).
  
  
  METHODS
  Referring to mature miR-21 sequence, the sense and antisense oligonucleotide (sense-miR-21 and AS-miR-21) modified by 2'O-Me were designed to transfect into TSCC cells (Tca8113 and high metastasis cells) by liposome transfection technology, in order to establish an in vitro TSCC cell model. The expression changes of miR-21 in the transfected cells were detected with real-time fluorescence quantitative polymerase chain reaction (real-time PCR). The changes of cell proliferation, cell cycle, cell early apoptosis, cell migration and invasion capabilities were detected respectively by the technologies of methyl thiazolyl tetrazolium (MTT), flow cytometry, Annexin V cell early apoptosis assay, scratch assay and Transwell assay, to check AS-miR-21's effect on the biological characteristics of human TSCC cell lines.
  
  
  RESULTS
  For the TSCC cells, the antisense oligonucleotide of targeting miR-21 could effectively inhibit cell proliferation, promoted cell apoptosis, and inhibited the capability of cell's migration and invasion.
  
  
  CONCLUSIONS
  The expressions of miR-21 decrease after AS-miR-21 transfected into TSCC cells, and miR-21 can affect biological behavior of TSCC cells.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.forename: Longjiang
authors.initials: L
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Xu
authors.forename: Pin
authors.initials: P
authors.lastname: Lü
authors.forename: Wenlong
authors.initials: W
authors.lastname: Zheng
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D035683:MicroRNAs; D012313:RNA; D060888:Real-Time Polymerase Chain Reaction; D014062:Tongue Neoplasms; D014162:Transfection
chemical_list: D035683:MicroRNAs; D012313:RNA