title: [Correlation between interleukin-28B genetic polymorphisms and primary hepatocellular carcinoma].
abstract: OBJECTIVE
  To explore the correlation between single nucleotide polymorphisms (SNPs) of interleukin-28B (IL-28B) gene and the susceptibility to primary hepatocellular carcinoma (HCC).
  
  
  METHODS
  A total of 300 histologically confirmed HCC cases (from November 2001 to April 2010) and 310 healthy controls with no history of chronic hepatitis B or hepatocellular carcinoma (2009-2010) were selected from a hospital in Guilin and a hospital in Beijing for this case-control study.139 HCC patients in the case group had complete clinical tracking data. All the subjects were Han Chinese, with no age or gender restrictions.2 ml peripheral blood samples were drawn from each subject with informed consent. SNP of rs12972991, rs4803223, rs8099917 and rs12979860 four loci in IL-28B gene were analyzed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF).
  
  
  RESULTS
  The frequencies of C allele at rs12972991, G allele at rs8099917 and G allele at rs4803223 were 6.7% (40/598), 7.9% (47/598) and 10.0% (59/588) respectively in case group; all higher than the corresponding frequencies in control group, separately 2.9% (18/618), 4.1% (25/616) and 3.6% (21/608). The differences were statistically significant (χ2=9.542, 7.858, 20.736, P values all<0.05). The above alleles could increase the risk of HCC, and the OR (95%CI) values were separately 1.67 (1.13-2.46), 1.49 (1.08-2.06) and 2.91 (1.79-4.72). The genotype frequencies of AC+CC at rs12972991, GT+GG at rs8099917, GA+GG at rs4803223 were 13.0% (39/299), 14.7% (44/299) and 19.0% (56/296) respectively in case group; while the frequencies were lower in control group, separately 5.8% (18/309), 8.1% (25/308) and 6.6% (20/304). The differences were statistically significant (χ2=9.319, 6.557, 20.948, P values all<0.05). These genotypes may increase the risk of HCC, and the adjusted OR (95%CI) values were 2.24 (1.31-3.83), 1.81 (1.14-2.88) and 2.90 (1.78-4.70), respectively. The stratified analysis of the clinical data indicated that the frequency of genotype GA+GG at rs4803223 was 50.0% (13/26) in patients of tumor thrombosis in portal vein (TTPV), higher than the frequency of genotype AA (21.1%, 23/109). The difference was statistically significant (χ2=8.965, P=0.003).
  
  
  CONCLUSIONS
  The results suggested that IL-28B gene polymorphisms was correlated to the susceptibility to HCC in Chinese Han ethnic population. Among them, GA + GG genotype at rs4803223 could increase the risk of TTPV in HCC patients.
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Hospital, Guilin Medical College, Guilin 541001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Heng-hui
authors.initials: HH
authors.lastname: Zhang
authors.forename: Yan-hui
authors.initials: YH
authors.lastname: Chen
authors.forename: Xing-wang
authors.initials: XW
authors.lastname: Xie
authors.forename: Wei-jia
authors.initials: WJ
authors.lastname: Liao
authors.forename: Li-ling
authors.initials: LL
authors.lastname: Qin
authors.forename: Xiu-yun
authors.initials: XY
authors.lastname: Sun
authors.forename: Ran
authors.initials: R
authors.lastname: Fei
authors.forename: Xue-yan
authors.initials: XY
authors.lastname: Wang
authors.forename: Lai
authors.initials: L
authors.lastname: Wei
authors.forename: Hong-song
authors.initials: HS
authors.lastname: Chen
authors.forename: Ming-hui
authors.initials: MH
authors.lastname: Mei
mesh_terms: D000483:Alleles; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D000095022:Interferon Lambda
chemical_list: D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda