title: TGF-β polymorphism and its expression correlated with CXCR4 expression in human breast cancer.
abstract: The role of chemokines and the growth factors has been extensively analyzed both in cancer risk and tumor progression. The transforming growth factor beta (TGF-β) and chemokine (C-X-C motif) receptor 4 (CXCR4) genes are implicated in several diseases, including breast cancer. Genomic DNA was obtained from 21 samples of peripheral blood or from normal tissue, previously fixed in formalin and embedded in paraffin for TGF-β T869C polymorphism analyses. Total cellular RNA was extracted from the same 21 patients, but from fresh tissue (tumor and adjacent healthy from the same breast) for expression analysis by Real Time PCR. No significant differences were observed in genotype distribution according to clinicopathological characteristics. Transforming growth factor beta (TGF-β) mRNA expression was assessed according to T869C polymorphism and CC patients presented a higher TGF-β expression but not significant when compared to other genotypes (p = 0.064). A positive correlation was observed in relative mRNA expressions of CXCR4 and TGF-β (p = 0.020). It is known that overexpression of TGF-β by both tumor and stromal tissue can facilitate the development of metastases, mainly by TGF-β stimulated angiogenesis and increased tumor cell motility. Our findings suggested a role of these genes as progression markers for breast carcinoma.
authors.affiliation: Department of Pathological Sciences, Biological Sciences Center, State University of Londrina, Londrina, PR, CEP 86051-990, Brazil.
authors.forename: Julie Massayo Maeda
authors.initials: JM
authors.lastname: Oda
authors.forename: Karen Brajão
authors.initials: KB
authors.lastname: de Oliveira
authors.forename: Roberta Losi
authors.initials: RL
authors.lastname: Guembarovski
authors.forename: Kalil William Alves
authors.initials: KW
authors.lastname: de Lima
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: da Silva do Amaral Herrera
authors.forename: Alda Losi
authors.initials: AL
authors.lastname: Guembarovski
authors.forename: Walter Jorge
authors.initials: WJ
authors.lastname: Sobrinho
authors.forename: Daniela Rudgeri
authors.initials: DR
authors.lastname: Derossi
authors.forename: Maria Angelica Ehara
authors.initials: MA
authors.lastname: Watanabe
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D056726:Genetic Association Studies; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C527276:CXCR4 protein, human; D012333:RNA, Messenger; D019718:Receptors, CXCR4; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1