title: Enhancing specific binding of L929 fibroblasts: effects of multi-scale topography of GRGDY peptide modified surfaces.
abstract: Surface chemical composition and roughness caused by micro/nano-topographical structures are two predominant factors in determining cellular response. A series of gold nanoparticle layers (GNPLs) with increasing surface roughness are prepared by depositing gold nanoparticles onto planar gold films. Glycine-arginine-glycine-aspartic acid-tyrosine (GRGDY) peptide is then conjugated to the surface through poly[oligo(ethylene glycol) methacrylate] polymer brushes as spacers. The pristine micro/nano-roughness structures significantly inhibit L929 cell growth. After modification with POEGMA-GRGDY, however, the micro/nano-structures greatly enhance L929 cell-specific interactions while maintaining superior low-fouling ability compared to planar gold.
authors.affiliation: Jiangsu Key Laboratory of Advanced Functional Polymer Design and Application, Department of Polymer Science and Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Zhaoqiang
authors.initials: Z
authors.lastname: Wu
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005340:Fibrinogen / Q000737:chemistry; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D005353:Fibronectins / Q000737:chemistry; D006046:Gold; D053768:Metal Nanoparticles; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D005353:Fibronectins; D008689:Methacrylates; D009842:Oligopeptides; C578451:glycyl-arginyl-glycyl-aspartyl-tyrosine; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D006046:Gold; D005340:Fibrinogen