title: Protocol for obtaining platelet-rich plasma (PRP), platelet-poor plasma (PPP), and thrombin for autologous use.
abstract: BACKGROUND
  Plasma has been widely studied and used in many different situations to speed up healing with better tissue adherence and hemostasis. Research projects are now attempting to isolate platelet-rich plasma (PRP) and platelet-poor plasma (PPP), making better use of their properties, particularly during operations and for wounds that are slow to heal. In view of the wide diversity of industrial machines and extraction protocols, together with the variety of industrially produced biologic glues, this article suggests an option for obtaining PRP, PPP, and human thrombin for autologous use.
  
  
  METHODS
  A way of obtaining PRP, PPP, and thrombin is reproduced through a protocol defined and established by the authors. Autologous thrombin and plasma were obtained through the collection and successive centrifugation of ten whole blood samples, until the desired hemocomponents were isolated, followed by quantitative and qualitative analyses of the elements obtained.
  
  
  RESULTS
  The mean platelet concentration obtained was 6.03 × 10(8) platelets/ml, with a mean thrombin concentration of 33.54 nM, both values compatible with reports in the literature when different protocols are applied.
  
  
  CONCLUSIONS
  The protocol described is a good option for the preparation and application of PRP, PPP, and autologous thrombin, particularly as they can be obtained simultaneously, eliminating the possibilities of viral contamination and allergic reactions. Moreover, the cost of this procedure is low, it is easy to perform, and replicable.
  
  
  METHODS
  This journal requires that authors assign a level of evidence to each article.
authors.affiliation: Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. diogo@openlink.com.br
authors.forename: Diogo
authors.initials: D
authors.lastname: Franco
authors.forename: Talita
authors.initials: T
authors.lastname: Franco
authors.forename: Angélica Maria
authors.initials: AM
authors.lastname: Schettino
authors.forename: João Medeiros Tavares
authors.initials: JM
authors.lastname: Filho
authors.forename: Fabiel Spani
authors.initials: FS
authors.lastname: Vendramin
mesh_terms: D001804:Blood Transfusion, Autologous; D002985:Clinical Protocols; D006801:Humans; D053657:Platelet-Rich Plasma; D013917:Thrombin
chemical_list: D013917:Thrombin