title: miR-203 inhibits the migration and invasion of esophageal squamous cell             carcinoma by regulating LASP1.
abstract: The expression of microRNA-203 (miR-203) in esophageal squamous cell carcinoma             (ESCC) tissues is remarkably lower than that in non‑ESCC tissues. We investigated             how miR-203 could influence the development of ESCC cells. Our analyses revealed             that miR-203 inhibited the migration and invasion of ESCC cells. Genome-wide gene             expression data and target site inhibition assays showed that miR-203 appears             to directly regulate LIM and SH3 protein 1 (LASP1). The knockdown of LASP1 resulted             in inhibition of the migration and invasion of ESCC cells. Our results suggest             that miR-203 and its target LASP1, may be associated with the progression of ESCC.             In clinical ESCC specimens, the expression levels of miR-203, which were lower             compared to those in normal tissues, were inversely correlated with the mRNA expression             levels of LASP1. Moreover, we found that there was a significant correlation between             the expression levels of miR-203 and the relapse‑free survival. The identification             of a cancer network regulated by miR-203 could provide new insights into the potential             mechanisms of the progression of ESCC.
authors.affiliation: Department of Frontier Surgery, Chiba University Graduate School                     of Medicine, Chiba, Japan.
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Takeshita
authors.forename: Mikito
authors.initials: M
authors.lastname: Mori
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kano
authors.forename: Isamu
authors.initials: I
authors.lastname: Hoshino
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Akutsu
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Hanari
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoneyama
authors.forename: Norimasa
authors.initials: N
authors.lastname: Ikeda
authors.forename: Yuka
authors.initials: Y
authors.lastname: Isozaki
authors.forename: Tetsurou
authors.initials: T
authors.lastname: Maruyama
authors.forename: Naoki
authors.initials: N
authors.lastname: Akanuma
authors.forename: Yukimasa
authors.initials: Y
authors.lastname: Miyazawa
authors.forename: Hisahiro
authors.initials: H
authors.lastname: Matsubara
mesh_terms: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics*; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D060588:LIM Domain Proteins / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; C096353:LASP1 protein, human; D060588:LIM Domain Proteins; C546703:MIRN203 microRNA, human; D035683:MicroRNAs