title: H(2) O(2) - and pH-sensitive CdTe quantum dots as fluorescence probes for the detection of glucose.
abstract: A novel fluorescence assay system for glucose was developed with thioglycollic acid (TGA)-capped CdTe quantum dots (QDs) as probes. The luminescence quantum yield of the TGA-capped CdTe QDs was highly sensitive to H2 O2 and pH. In the presence of glucose oxidase, glucose is oxidized to yield, gluconic acid and H2 O2 . H2 O2 and H(+) (dissociated from gluconic acid) intensively quenched the fluorescence of QDs. The experimental results showed that the quenched fluorescence was proportional to the glucose concentration within the range of 0.01-5.0 mm under optimized experimental conditions. Compared with most of the existing methods, this newly developed system possesses many advantages, including simplicity, low cost, high flexibility, and good sensitivity. Furthermore, no complicated chemical modification of QDs and enzyme immobilization was needed in this system.
authors.affiliation: Key laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Xi'an, 710062, China.
authors.forename: Yinping
authors.initials: Y
authors.lastname: Li
authors.forename: Baoxin
authors.initials: B
authors.lastname: Li
authors.forename: Junli
authors.initials: J
authors.lastname: Zhang
mesh_terms: D019187:Cadmium Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D013691:Tellurium / Q000737:chemistry*; D013864:Thioglycolates / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D013864:Thioglycolates; C017487:2-mercaptoacetate; D006861:Hydrogen Peroxide; D005947:Glucose; D013691:Tellurium; C028337:cadmium telluride