title: Human saphenous vein endothelial cell adhesion and expansion on micropatterned polytetrafluoroethylene.
abstract: Intimal hyperplasia and thrombosis are responsible for the poor patency rates of small-diameter vascular grafts. These complications could be avoided by a rapid and strong adhesion of endothelial cells to the prosthetic surfaces, which typically consist of expanded polytetrafluoroethylene (PTFE) for small-diameter vessels. We have previously described two peptide micropatterning strategies that increase the endothelialization rates of PTFE. The micropatterns were generated either by inkjet printing 300 μm squares or by spraying 10.1 ± 0.1 μm diameter droplets of the CGRGDS cell adhesion peptide, while the remaining surface was functionalized using the CWQPPRARI cell migration peptide. We now directly compare these two micropatterning strategies and examine the effect of hydrodynamic stress on human saphenous vein endothelial cells grown on the patterned surfaces. No significant differences in cell adhesion were observed between the two micropatterning methods. When compared to unpatterned surfaces treated with a uniform mixture of the two peptides, the cell expansion was significantly higher on sprayed or printed surfaces after 9 days of static cell culture. In addition, after 6 h of exposure to hydrodynamic stress, the cell retention and cell cytoskeleton reorganization on the patterned surfaces was improved when compared to untreated or random treated surfaces. These results indicate that micropatterned surfaces lead to improved rates of PTFE endothelialization with higher resistance to hydrodynamic stress.
authors.affiliation: Laboratoire d'ingénierie de surface, Centre de Recherche sur les Matériaux Avancés, Département de génie des mines, de la métallurgie et des matériaux, Université Laval, Québec, Canada.
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Boivin
authors.forename: Pascale
authors.initials: P
authors.lastname: Chevallier
authors.forename: Corinne A
authors.initials: CA
authors.lastname: Hoesli
authors.forename: Jean
authors.initials: J
authors.lastname: Lagueux
authors.forename: Reine
authors.initials: R
authors.lastname: Bareille
authors.forename: Murielle
authors.initials: M
authors.lastname: Rémy
authors.forename: Laurence
authors.initials: L
authors.lastname: Bordenave
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Durrieu
authors.forename: Gaétan
authors.initials: G
authors.lastname: Laroche
mesh_terms: D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D057446:Hydrodynamics; D010455:Peptides / Q000737:chemistry*; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D012501:Saphenous Vein / Q000166:cytology / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D010455:Peptides; D011138:Polytetrafluoroethylene