title: Antimicrobial and anti-biofilm properties of new taxodione derivative from hairy roots of Salvia austriaca.
abstract: The aim of the present report was to evaluate antimicrobial/anti-biofilm activity of 7-(2-oxohexyl)-taxodione, a novel taxodione derivative isolated from n-hexane extract of Salvia austriaca hairy roots. Antimicrobial assays showed that 7-(2-oxohexyl)-taxodione was at least 4 times more active than taxodione against methicillin-susceptible as well against methicillin-resistant staphylococci with MIC of 1.25-2.5 μgml(-1). This compound was less active against vancomycin-resistant enterococci (VRE), on the same level as taxodione (MIC ranged 10.0-20.0 μgml(-1)). The presence of 7-(2-oxohexyl)-taxodione in the culture medium (at MIC, ½ MIC or ¼ MIC) decreased adhesion of staphylococci to abiotic surfaces, which in turn caused a reduction in biofilm formation during 24h, by approximately 25-30%. Also, the extent of established biofilm eradication was found to be significant, although it required an increased concentration of the compound. This is the first report on the antimicrobial activity of this, up to now not known compound, isolated from transformed roots of S. austriaca.
authors.affiliation: Department of Biology and Pharmaceutical Botany, Medical University of Łódź, Łódź, Poland.
authors.forename: L
authors.initials: L
authors.lastname: Kuźma
authors.forename: H
authors.initials: H
authors.lastname: Wysokińska
authors.forename: M
authors.initials: M
authors.lastname: Różalski
authors.forename: A
authors.initials: A
authors.lastname: Budzyńska
authors.forename: M
authors.initials: M
authors.lastname: Więckowska-Szakiel
authors.forename: B
authors.initials: B
authors.lastname: Sadowska
authors.forename: M
authors.initials: M
authors.lastname: Paszkiewicz
authors.forename: W
authors.initials: W
authors.lastname: Kisiel
authors.forename: B
authors.initials: B
authors.lastname: Różalska
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D016983:Enterococcus / Q000187:drug effects; D016106:Methicillin Resistance / Q000187:drug effects; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010616:Phenanthrenes / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D027544:Salvia / Q000737:chemistry*; D013210:Staphylococcus / Q000187:drug effects* / Q000472:pathogenicity; D020713:Vancomycin Resistance / Q000187:drug effects
chemical_list: C580628:7-(2-oxohexyl)taxodione; D000900:Anti-Bacterial Agents; D004224:Diterpenes; D010616:Phenanthrenes; D010936:Plant Extracts; C009387:taxodione