title: Proteomic profiling of osteosarcoma cells identifies ALDOA and SULT1A3 as negative survival markers of human osteosarcoma.
abstract: Osteosarcoma (OSA) is the most common primary malignancy of bone. Molecular mechanism underlying OSA remains to be fully elucidated. It is critical to identify reliable diagnostic and prognostic markers for OSA at the molecular levels. This study is designed to investigate possible molecular mechanisms behind OSA development and to identify novel prognostic markers related to OSA survival. We conduct a comprehensive proteomic profiling analysis of human OSA cell lines with differential metastatic potential. Through comprehensive combinatorial analyses of the proteomic data and the previously obtained cDNA microarray results, we identify 37 candidate proteins which are differentially expressed in OSA sublines. Among them, ALDOA and SULT1A3 are selected for further investigation. The expressions of protein are confirmed by Western blotting analysis. We further analyze the expression levels of ALDOA and SULT1A3 from 40 clinical cases of OSA. The results demonstrate that the expression of ALDOA and/or SULT1A3 is significantly higher in patients with worse survival time than patients with better survival time. Five-year survival analysis shows there is a statistically significant difference between two patient populations. The data strongly suggest that ALDOA and/or SULT1A3 expression level in biopsy samples may predict the clinical outcomes of OSA patients. Furthermore, the biological functions of ALDOA and SULT1A3 may be implicated in OSA development and/or progression.
authors.affiliation: Center of Orthopedic Surgery, The Orthopedics Oncology Institute of Chinese PLA, The Affiliated Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.forename: Tong-Tao
authors.initials: TT
authors.lastname: Yang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xiu-Chun
authors.initials: XC
authors.lastname: Qiu
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Cun-Xiao
authors.initials: CX
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Long
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Ma
authors.forename: Qiong
authors.initials: Q
authors.lastname: Ma
authors.forename: Xian-zhi
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Lian-Jia
authors.initials: LJ
authors.lastname: Yang
authors.forename: Qing-Yu
authors.initials: QY
authors.lastname: Fan
mesh_terms: D015239:Arylsulfotransferase / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D015239:Arylsulfotransferase; C403170:monoamine-sulfating phenol sulfotransferase; D005634:Fructose-Bisphosphate Aldolase