title: In vivo efficacy of a silicone‒cationic steroid antimicrobial coating to prevent implant-related infection.
abstract: Active release antimicrobial coatings for medical devices have been developed to prevent and treat biofilm implant-related infections. To date, only a handful of coatings have been put into clinical use, with limited success. In this study, a novel antimicrobial compound was incorporated into a silicone (polydimethylsiloxane or PDMS) polymer to develop a novel active release coating that addressed several limitations of current device coatings. The efficacy of this coating was optimized using an in vitro flow cells system, then translated to an animal model of a simulated Type IIIB open fracture wherein well-established biofilms were used as initial inocula. Results indicated that the novel coating was able to prevent infection in 100% (9/9) of animals that were treated with biofilms and the novel coating (treatment group). In contrast, 100% (9/9) of animals that were inoculated with biofilms and not treated with the coating (positive control), did develop infection. Nine animals were used as negative controls, i.e., those that were not treated with biofilms, and showed a rate of infection of 11% (1/9). Eight animals were treated with the novel coating only to determine its effect on host tissue. Results indicated that the novel active release coating may have significant promise for future application to prevent biofilm implant-related infections in patients.
authors.affiliation: George E. Wahlen Department of Veterans Affairs Medical Center, Salt Lake City, UT 84148, USA. dustin.williams@utah.edu
authors.forename: Dustin L
authors.initials: DL
authors.lastname: Williams
authors.forename: Bryan S
authors.initials: BS
authors.lastname: Haymond
authors.forename: James P
authors.initials: JP
authors.lastname: Beck
authors.forename: Paul B
authors.initials: PB
authors.lastname: Savage
authors.forename: Vinod
authors.initials: V
authors.lastname: Chaudhary
authors.forename: Richard T
authors.initials: RT
authors.lastname: Epperson
authors.forename: Brooke
authors.initials: B
authors.lastname: Kawaguchi
authors.forename: Roy D
authors.initials: RD
authors.lastname: Bloebaum
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000627:therapeutic use*; D018441:Biofilms / Q000187:drug effects*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D008855:Microscopy, Electron, Scanning; D019736:Prostheses and Implants / Q000382:microbiology*; D012756:Sheep; D012828:Silicones / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry* / Q000627:therapeutic use*
chemical_list: D000890:Anti-Infective Agents; D004129:Dimethylpolysiloxanes; D012828:Silicones; D013256:Steroids; C013830:baysilon