title: Effect of tissue specificity on the performance of extracellular matrix in improving endothelialization of cardiovascular implants.
abstract: Natural extracellular matrix (ECM) deposited in situ by cultured endothelial cells (ECs) has been proven effective in accelerating endothelialization of titanium (Ti) cardiovascular implants (CVIs) in our previous studies. In this study, the ECM deposited by smooth muscle cells (SMCs) was used in comparison to investigate the effects of tissue specificity of the ECM on the ability to accelerate endothelialization of CVIs. The results demonstrated that the ECM deposited by ECs and SMCs (EC-ECM, SMC-ECM, respectively) differed considerably in components and fibril morphology. Surface modification of Ti CVIs with both types of natural ECM was effective in improving their in vitro hemocompatibility and cytocompatibility simultaneously. However, the endothelialization of ECM-modified Ti CVIs in a canine model demonstrated a high tissue specificity of the ECM. Although the ECM deposited by SMCs (SMC-ECM) induced fewer platelet adhesion and sustained better growth and viability of ECs in vitro, its performance in accelerating in vivo endothelialization of Ti CVIs was extremely poor. In contrast, the ECM deposited by ECs (EC-ECM) led to complete endothelium formation in vivo.
authors.affiliation: Key Laboratory of Advanced Technology of Materials, Education Ministry of China, Southwest Jiaotong University, Chengdu, China.
authors.forename: Qiufen
authors.initials: Q
authors.lastname: Tu
authors.forename: Zhilu
authors.initials: Z
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Kaiqin
authors.initials: K
authors.lastname: Xiong
authors.forename: Manfred F
authors.initials: MF
authors.lastname: Maitz
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Yuancong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Jin
authors.forename: Yuechang
authors.initials: Y
authors.lastname: Lei
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D002478:Cells, Cultured; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology* / Q000254:growth & development*; D005109:Extracellular Matrix / Q000378:metabolism*; D005263:Femoral Artery / Q000166:cytology* / Q000378:metabolism / Q000601:surgery; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D009928:Organ Specificity / Q000502:physiology; D054457:Tissue Scaffolds