title: Drug screening study using glioma stem-like cells.
abstract: Glioma is one of the most common malignant tumors affecting the central             nervous system. We screened active drugs from 12 classes to provide experimental             data for the study of human glioma. Serum-free suspension clone formation was             used to induce the formation of glioma stem‑like cells (GSLCs) from U251 cells.             We screened active drugs from 12 classes and observed their effects on the number             of tumor balls. The results revealed that serum‑free suspension clone formation             successfully induced the formation of GSLCs. Six active drugs [curcumin, chrysin,             apigenin, luteolin, casticin and 8-bromo-7-methoxychrysin (BrMChR)] were identified             by MTT assay. The drugs that affected the number of tumor balls were curcumin,             luteolin, casticin and BrMChR (all P<0.05). The GSLCs were obtained by serum‑free             clone suspension formation and exhibited the features of cancer stem cells.
authors.affiliation: College of Medicine, Hunan Normal University, Changsha, Hunan 410006, PR China. fengxing01@hotmail.com
authors.forename: Xing
authors.initials: X
authors.lastname: Feng
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
authors.forename: Chong
authors.initials: C
authors.lastname: Liu
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Tao
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001932:Brain Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D005910:Glioma / Q000188:drug therapy / Q000473:pathology; D006801:Humans; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D005419:Flavonoids