title: Developmental validation of the PowerPlex® 18D System, a rapid STR multiplex for analysis of reference samples.
abstract: As short tandem repeat markers remain the foundation of human identification throughout the world, new STR multiplexes require rigorous testing to ensure the assays are sufficiently robust and reliable for genotyping purposes. The PowerPlex(®) 18D System was created for the direct amplification of buccal and blood samples from FTA(®) storage cards and reliably accommodates other sample materials. The PowerPlex(®) 18D System allows simultaneous amplification of the 13 CODIS loci and amelogenin along with four additional loci: Penta E, Penta D, D2S1338, and D19S433. To demonstrate suitability for human identification testing, the PowerPlex(®) 18D System was tested for sensitivity, concordance, inhibitor tolerance, and performance with thermal cycling and reaction condition variation following SWGDAM developmental validation guidelines. Given these results, PowerPlex(®) 18D System can confidently be used for forensic and human identification testing.
authors.affiliation: Promega Corporation, 2800 Woods Hollow Road, Madison, WI 53711, USA. katie.oostdik@promega.com
authors.forename: Kathryn
authors.initials: K
authors.lastname: Oostdik
authors.forename: Julie
authors.initials: J
authors.lastname: French
authors.forename: Donald
authors.initials: D
authors.lastname: Yet
authors.forename: Briana
authors.initials: B
authors.lastname: Smalling
authors.forename: Craig
authors.initials: C
authors.lastname: Nolde
authors.forename: Peter M
authors.initials: PM
authors.lastname: Vallone
authors.forename: Erica L R
authors.initials: EL
authors.lastname: Butts
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Hill
authors.forename: Margaret C
authors.initials: MC
authors.lastname: Kline
authors.forename: Theresa
authors.initials: T
authors.lastname: Rinta
authors.forename: Amy M
authors.initials: AM
authors.lastname: Gerow
authors.forename: Stacey R
authors.initials: SR
authors.lastname: Allen
authors.forename: Christopher K
authors.initials: CK
authors.lastname: Huber
authors.forename: John
authors.initials: J
authors.lastname: Teske
authors.forename: Benjamin
authors.initials: B
authors.lastname: Krenke
authors.forename: Martin
authors.initials: M
authors.lastname: Ensenberger
authors.forename: Patricia
authors.initials: P
authors.lastname: Fulmer
authors.forename: Cynthia
authors.initials: C
authors.lastname: Sprecher
mesh_terms: D019075:Electrophoresis, Capillary; D018732:Forensic Anthropology / Q000379:methods*; D005787:Gene Frequency; D006801:Humans; D018895:Microsatellite Repeats; D012015:Reference Standards