title: Sequence characterization of heat shock protein gene of Cyclospora cayetanensis isolates from Nepal, Mexico, and Peru.
abstract: We have described the development of a 2-step nested PCR protocol based on the characterization of the 70-kDa heat shock protein (HSP70) gene for rapid detection of the human-pathogenic Cyclospora cayetanensis parasite. We tested and validated these newly designed primer sets by PCR amplification followed by nucleotide sequencing of PCR-amplified HSP70 fragments belonging to 16 human C. cayetanensis isolates from 3 different endemic regions that include Nepal, Mexico, and Peru. No genetic polymorphism was observed among the isolates at the characterized regions of the HSP70 locus. This newly developed HSP70 gene-based nested PCR protocol provides another useful genetic marker for the rapid detection of C. cayetanensis in the future.
authors.affiliation: Microbiological Sciences Branch, Southeast Regional Laboratory, Food and Drug Administration, 60, Eighth Street NE, Atlanta, Georgia 30309, USA. irshad.sulaiman@fda.hhs.gov
authors.forename: Irshad M
authors.initials: IM
authors.lastname: Sulaiman
authors.forename: Patricia
authors.initials: P
authors.lastname: Torres
authors.forename: Steven
authors.initials: S
authors.lastname: Simpson
authors.forename: Khalil
authors.initials: K
authors.lastname: Kerdahi
authors.forename: Ynes
authors.initials: Y
authors.lastname: Ortega
mesh_terms: D001483:Base Sequence; D021744:Cyclospora / Q000737:chemistry / Q000235:genetics*; D021866:Cyclosporiasis / Q000469:parasitology*; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D005819:Genetic Markers; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008800:Mexico; D008969:Molecular Sequence Data; D009390:Nepal; D010568:Peru; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015800:Protozoan Proteins / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D016054:DNA, Protozoan; D005819:Genetic Markers; D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins