title: Relation between expression pattern of wild-type p53 and multidrug resistance proteins in human nephroblastomas.
abstract: One of the best characterized resistance mechanisms of human cancer is multidrug resistance (MDR) mediated by P-glycoprotein (Pgp/MDR1) and multidrug-resistant related protein (MRP1). In addition to Pgp/MDR1 and MRP1, p53 inactivation or mutation might play a relevant role in therapeutic failure. This study involved 25 children (17 girls and 8 boys) aged 7 months to 10 years treated for unilateral Wilms' tumor. 25 tissue samples of Wilms' tumor and 5 samples of normal human kidneys were obtained from the Department of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, Slovak Republic. We used an indirect immunohistochemical method to determine expression of Pgp/MDR1, MRP1 and wild-type p53 in 25 tissue samples of nephroblastoma. The minority of nephroblastoma specimens showed positivity for both MDR proteins, as well as for wild-type p53. 24% of tissue samples revealed positive results for Pgp/MDR1, 48% for MRP1 and 8% for wild-type p53. Furthermore, our study showed a statistically significant difference between p53 and MRP1 protein expression (p<0.01), but not between p53 and Pgp/MDR1 (p>0.05). No correlation was found between the expression of both multidrug resistance proteins (Pgp/MDR1 and MRP1) and the expression of wild-type p53. Immunohistochemical detection of the expression of MDR proteins and wild-type p53 at the time of diagnosis might assist in choosing specific chemotherapeutics to improve prognosis and therapy.
authors.affiliation: Department of Anatomy, P.J. Šafárik University, Faculty of Medicine, Šrobárova 2, SK-04180 Košice, Slovak Republic.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Hodorová
authors.forename: Silvia
authors.initials: S
authors.lastname: Rybárová
authors.forename: Janka
authors.initials: J
authors.lastname: Vecanová
authors.forename: Peter
authors.initials: P
authors.lastname: Solár
authors.forename: Lukáš
authors.initials: L
authors.lastname: Plank
authors.forename: Jozef
authors.initials: J
authors.lastname: Mihalik
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000032:analysis / Q000096:biosynthesis; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000032:analysis* / Q000096:biosynthesis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000096:biosynthesis; D009396:Wilms Tumor / Q000378:metabolism* / Q000473:pathology
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D016159:Tumor Suppressor Protein p53; C513055:ABCB1 protein, human; C421109:multidrug resistance-associated protein 1