title: An NMR metabolomics approach for the diagnosis of leptomeningeal carcinomatosis.
abstract: Leptomeningeal carcinomatosis (LC) is the third most common metastatic complication of the central nervous system. However, the current modalities to reliably diagnose this condition are not satisfactory. Here, we report a preclinical proof of concept for a metabolomics-based diagnostic strategy using a rat LC model incorporating glioma cells that stably express green fluorescent protein. Cytologic diagnoses gave 66.7% sensitivity for the 7-day LC group and 0% for the 3-day LC group. MR imaging could not diagnose LC at these stages. In contrast, nuclear magnetic resonance-based metabolomics on cerebrospinal fluid detected marked differences between the normal and LC groups. Predictions based on the multivariate model provided sensitivity, specificity, and overall accuracy of 88% to 89% in both groups for LC diagnosis. Further statistical analyses identified lactate, acetate, and creatine as specific for the 7-day LC group, with glucose a specific marker of the normal group. Overall, we showed that the metabolomics approach provided both earlier and more accurate diagnostic results than cytology and MR imaging in current use.
authors.affiliation: Department of Radiology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Hye Rim
authors.initials: HR
authors.lastname: Cho
authors.forename: He
authors.initials: H
authors.lastname: Wen
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Ryu
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: An
authors.forename: Hyo Cheol
authors.initials: HC
authors.lastname: Kim
authors.forename: Woo Kyung
authors.initials: WK
authors.lastname: Moon
authors.forename: Moon Hee
authors.initials: MH
authors.lastname: Han
authors.forename: Sunghyouk
authors.initials: S
authors.lastname: Park
authors.forename: Seung Hong
authors.initials: SH
authors.lastname: Choi
mesh_terms: D000818:Animals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055756:Meningeal Carcinomatosis / Q000175:diagnosis*; D055432:Metabolomics; D051381:Rats; D011916:Rats, Inbred F344