title: Generation and evaluation of the efficacy of rhesus monkey soluble cytotoxic T lymphocyte-associated antigen-4 in the allogeneic mixed lymphocyte reaction.
abstract: Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4; CD152) is a transmembrane protein that is structurally similar to CD28. As CTLA-4 has a much higher binding affinity to B7 than CD28, several approaches using soluble CTLA-4 have been tried to down-regulate T cell activity by blocking the interaction between CD28 and B7. We constructed soluble rhesus monkey CTLA-4 immunoglobulin (CTLA-4Ig) containing a critical binding site to B7 combined with a constant Ig heavy chain region in a mammalian system. Flow cytometry analyses indicated that soluble rhesus monkey CTLA-4Ig bound to rhesus monkey CD86 (B7.2). Moreover, soluble rhesus monkey CTLA-4Ig more effectively blocked the rhesus monkey-rhesus monkey allogeneic mixed lymphocyte reaction compared with that of humans. These results indicate that soluble rhesus monkey CTLA-4Ig may be useful in preclinical trials in a rhesus monkey model.
authors.affiliation: Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jung-Sik
authors.initials: JS
authors.lastname: Kim
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
authors.forename: Han-Hyoung
authors.initials: HH
authors.lastname: Lee
authors.forename: Suk Jun
authors.initials: SJ
authors.lastname: Lee
authors.forename: Manheum
authors.initials: M
authors.lastname: Na
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Jihye
authors.initials: J
authors.lastname: Han
authors.forename: Joonbeom
authors.initials: J
authors.lastname: Bae
authors.forename: Sang-Pil
authors.initials: SP
authors.lastname: Choi
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Kim
authors.forename: Chung-Gyu
authors.initials: CG
authors.lastname: Park
authors.forename: Taehoon
authors.initials: T
authors.lastname: Chun
mesh_terms: D000818:Animals; D051940:B7-2 Antigen / Q000037:antagonists & inhibitors* / Q000276:immunology*; D060908:CTLA-4 Antigen / Q000235:genetics / Q000276:immunology*; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology*; D007155:Immunologic Factors / Q000235:genetics / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008253:Macaca mulatta
chemical_list: D051940:B7-2 Antigen; D060908:CTLA-4 Antigen; D007136:Immunoglobulins; D007155:Immunologic Factors