title: Polymerization-based signal amplification under ambient conditions with thirty-five second reaction times.
abstract: Although polymerization-based amplification (PBA) has demonstrated promise as an inexpensive technique for use in molecular diagnostics, oxygen inhibition of radical photopolymerization has hindered its implementation in point-of-care devices. The addition of 0.3-0.7 μM eosin to an aqueous acrylate monomer solution containing a tertiary amine allows an interfacial polymerization reaction to proceed in air only near regions of a test surface where additional eosin initiators coupled to proteins have been localized as a function of molecular recognition events. The dose of light required for the reaction is inversely related to eosin concentration. This system achieves sensitivities comparable to those reported for inert gas-purged systems and requires significantly shorter reaction times. We provide several comparisons of this system with other implementations of polymerization-based amplification.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Kaja
authors.initials: K
authors.lastname: Kaastrup
authors.forename: Hadley D
authors.initials: HD
authors.lastname: Sikes
mesh_terms: D004801:Eosine Yellowish-(YS) / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D010545:Peroxides / Q000737:chemistry*; D055668:Photochemical Processes
chemical_list: D009841:Oligonucleotides; D010545:Peroxides; C049375:perhydroxyl radical; D010100:Oxygen; D004801:Eosine Yellowish-(YS)