title: Product identification and safety evaluation of aflatoxin B1 decontaminated by electrolyzed oxidizing water.
abstract: In this study with aflatoxin-contaminated peanuts, the effectiveness of electrolyzed oxidizing water (EOW) in the decontamination of aflatoxin B(1) was investigated. The aflatoxin B(1) content was markedly reduced upon treatment with EOW, particularly with neutral electrolyzed oxidizing water (NEW). The conversion product of EOW treatment was isolated and identified as 8-chloro-9-hydroxy aflatoxin B(1) (compound 1), which is an amphiphilic molecule, in contrast to fat-soluble aflatoxin B(1). A mutagenic response study revealed that the number of revertants per plate after treatment of bacterial strains TA-97, TA-98, TA-100, and TA-102 with NEW was within the standard value range. The HepG2 cell viability assay yielded an IC(50) value of compound 1 approximately 150 mM. This study indicates that EOW had the ability to decontaminate aflatoxin B(1), and the conversion product, compound 1, did not exhibit mutagenic activity or cytotoxic effects.
authors.affiliation: College of Food Science and Nutritional Engineering,  China Agricultural University , P.O. Box 40, No. 17 Qing hua dong lu, Hai dian, Beijing 100083, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xiong
authors.forename: Hai jie
authors.initials: Hj
authors.lastname: Liu
authors.forename: Li te
authors.initials: Lt
authors.lastname: Li
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis / Q000737:chemistry*; D000348:Aflatoxins / Q000737:chemistry / Q000633:toxicity; D010367:Arachis / Q000737:chemistry* / Q000382:microbiology; D001231:Aspergillus flavus / Q000472:pathogenicity; D002851:Chromatography, High Pressure Liquid; D003666:Decontamination; D004572:Electrolysis / Q000379:methods*; D005506:Food Contamination; D059022:Food Safety / Q000379:methods*; D056945:Hep G2 Cells; D006801:Humans; D015394:Molecular Structure; D009152:Mutagenicity Tests / Q000379:methods; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
chemical_list: C580740:8-chloro-9-hydroxy aflatoxin B1; D000348:Aflatoxins; D016604:Aflatoxin B1