title: [Effect of astragalus polysaccharide on sensitivity of leukemic cell line HL-60 to NK cell cytotoxicity and its mechanism].
abstract: The objective of this study was to explore the effect of astragalus polysaccharide (APS) on sensitivity of leukemic cell line HL-60 to NK cell cytotoxicity and its mechanism. The cytotoxicities of NK cells against HL-60 cells were analyzed by LDH releasing assay at different effect-to-target cell ratios (E:T) before and after treated with APS. The gene expression of MHC class I chain-related (MICA) in HL-60 cells before and after APS treatment was assayed with RT-PCR. Protein expression of MICA in HL-60 cells was assayed by flow cytometry before and after treated by APS. The results showed that after treated with APS 15 mg/ml for 48 h, the cytotoxicities of NK cells against HL-60 cells enhanced at different effect-to-target (P < 0.05), and the gene and protein expressions in MICA of HL-60 cells were up-regulated (P < 0.05). It is concluded that the APS can obviously up-regulate the expression of MICA in HL-60 cells, thus enhance sensitivity of HL-60 cells to cytotoxicity of NK cells.
authors.affiliation: Department of Hematology, Second Hospital of Lanzhou University, Lanzhou, China.
authors.forename: Peng-Yun
authors.initials: PY
authors.lastname: Zeng
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Deng
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Yue
authors.forename: Lian-Sheng
authors.initials: LS
authors.lastname: Zhang
mesh_terms: D027883:Astragalus Plant; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D018922:HL-60 Cells; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D007694:Killer Cells, Natural; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D015395:Histocompatibility Antigens Class I; D011134:Polysaccharides