title: Rejuvenation of gene expression pattern of aged human skin by broadband light treatment: a pilot study.
abstract: Studies in model organisms suggest that aged cells can be functionally rejuvenated, but whether this concept applies to human skin is unclear. Here we apply 3'-end sequencing for expression quantification ("3-seq") to discover the gene expression program associated with human photoaging and intrinsic skin aging (collectively termed "skin aging"), and the impact of broadband light (BBL) treatment. We find that skin aging was associated with a significantly altered expression level of 2,265 coding and noncoding RNAs, of which 1,293 became "rejuvenated" after BBL treatment; i.e., they became more similar to their expression level in youthful skin. Rejuvenated genes (RGs) included several known key regulators of organismal longevity and their proximal long noncoding RNAs. Skin aging is not associated with systematic changes in 3'-end mRNA processing. Hence, BBL treatment can restore gene expression pattern of photoaged and intrinsically aged human skin to resemble young skin. In addition, our data reveal, to our knowledge, a previously unreported set of targets that may lead to new insights into the human skin aging process.
authors.affiliation: Department of Dermatology, Stanford University School of Medicine, Redwood City, California 94063, USA. alschang@stanford.edu
authors.forename: Anne Lynn S
authors.initials: AL
authors.lastname: Chang
authors.forename: Patrick H
authors.initials: PH
authors.lastname: Bitter
authors.forename: Kun
authors.initials: K
authors.lastname: Qu
authors.forename: Meihong
authors.initials: M
authors.lastname: Lin
authors.forename: Nicole A
authors.initials: NA
authors.lastname: Rapicavoli
authors.forename: Howard Y
authors.initials: HY
authors.lastname: Chang
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005542:Forearm; D005786:Gene Expression Regulation / Q000502:physiology* / Q000528:radiation effects*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics; D008666:Metalloendopeptidases / Q000235:genetics; D008875:Middle Aged; D010789:Phototherapy / Q000379:methods*; D010865:Pilot Projects; D026721:RNA 3' End Processing / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics; D012060:Rejuvenation / Q000502:physiology*; D015595:Skin Aging / Q000235:genetics*; D059467:Transcriptome
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D022661:RNA, Untranslated; D008666:Metalloendopeptidases; C120130:ZMPSTE24 protein, human