title: Monitoring I-SceI-induced double-strand break repair in DT40 cells.
abstract: The chicken B lymphocyte cell line DT40 is now a well-established system for studying DNA repair in vertebrates. Central to its utility is its ready genetic tractability allowing for the creation of isogenic mutants in any chosen gene. The combination of this genetic tractability with the I-SceI-induced double-strand break (DSB) repair assay, pioneered by the laboratory of Maria Jasin, has proven particularly powerful for dissecting the pathways of DSB repair in a vertebrate setting.
authors.affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
authors.forename: David du
authors.initials: Dd
authors.lastname: Plessis
authors.forename: Julian E
authors.initials: JE
authors.lastname: Sale
mesh_terms: D000818:Animals; D002460:Cell Line; D002645:Chickens; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism*; D018274:Electroporation; D005434:Flow Cytometry; D005821:Genetic Techniques; D059765:Homologous Recombination; D014162:Transfection
chemical_list: D015252:Deoxyribonucleases, Type II Site-Specific