title: Effect of inoculum to substrate ratio (I/S) on municipal solid waste anaerobic degradation kinetics and potential.
abstract: The goal of this study is to evaluate the impact of the inoculum to substrate ratio (I/S) on the anaerobic degradation potential of municipal solid waste (MSW). Reconstituted MSW samples were thus incubated under batch anaerobic conditions and inoculated with an increasing amount of inoculum originating from a mesophilic sludge digester. I/S tested values were 0 (no inoculum added), 0.015, 0.03, 0.06, 0.12, 0.25, 1, 2 and 4 (gVM(inoculum)/gVM(waste)). The results indicate that the apparent maximal rate of dissolved organic carbon accumulation is reached at I/S=0.12. Under this level, the hydrolysis process is limited by the concentration of biomass and can thus be described as first order kinetics phenomena with respect to biomass for I/S ratios below 0.12. The maximum methane production rate and the minimal latency are reached at a ratio of 2. In addition to that, both methane signature and ARISA show a shift in the methanogenic populations from hydrogenotrophic to acetoclastic.
authors.affiliation: CEMAGREF, Hydrosystems and Bioprocesses Unit, Parc de Tourvoie, 92163 Antony, France. anthony.boulanger@veolia.com
authors.forename: Anthony
authors.initials: A
authors.lastname: Boulanger
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Pinet
authors.forename: Marielle
authors.initials: M
authors.lastname: Bouix
authors.forename: Théodore
authors.initials: T
authors.lastname: Bouchez
authors.forename: Alicia A
authors.initials: AA
authors.lastname: Mansour
mesh_terms: D000693:Anaerobiosis; D019149:Bioreactors; D005740:Gases; D007700:Kinetics; D012037:Refuse Disposal / Q000379:methods*; D013997:Time Factors
chemical_list: D005740:Gases