title: Simultaneous measurement of adenosine, dopamine, acetylcholine and 5-hydroxytryptamine in cerebral mice microdialysis samples by LC-ESI-MS/MS.
abstract: A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous measurement of adenosine (ADE), dopamine (DA), acetylcholine (ACh) and 5-hydroxytryptamine (5-HT) in mouse brain microdialysates. High method sensitivity (LLOQ of 0.05nM) was achieved by optimization of chromatographic and mass spectrometric parameters. The method was fully validated for its sensitivity, selectivity, matrix effect and stability. The LC-MS/MS method was successfully applied to evaluate the effect of the systemic administration of cocaine or amphetamine on the extracellular levels of ADE, DA, ACh and 5-HT in the mouse nucleus accumbens by microdialysis.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università degli Studi di Modena e Reggio Emilia, Via Campi 183, 41125 Modena, Italy. giuseppe.cannazza@unimore.it
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cannazza
authors.forename: Marina M
authors.initials: MM
authors.lastname: Carrozzo
authors.forename: Addolorata S
authors.initials: AS
authors.lastname: Cazzato
authors.forename: Irina M
authors.initials: IM
authors.lastname: Bretis
authors.forename: Luigino
authors.initials: L
authors.lastname: Troisi
authors.forename: Carlo
authors.initials: C
authors.lastname: Parenti
authors.forename: Daniela
authors.initials: D
authors.lastname: Braghiroli
authors.forename: Stefania
authors.initials: S
authors.lastname: Guiducci
authors.forename: Michele
authors.initials: M
authors.lastname: Zoli
mesh_terms: D000109:Acetylcholine / Q000032:analysis*; D000241:Adenosine / Q000032:analysis*; D000661:Amphetamine / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003042:Cocaine / Q000737:chemistry / Q000494:pharmacology; D004298:Dopamine / Q000032:analysis*; D051379:Mice; D017551:Microdialysis / Q000379:methods; D009714:Nucleus Accumbens / Q000737:chemistry* / Q000187:drug effects; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D012701:Serotonin; D000661:Amphetamine; D003042:Cocaine; D000241:Adenosine; D000109:Acetylcholine; D004298:Dopamine