title: Noninvasive photoacoustic computed tomography of mouse brain metabolism in vivo.
abstract: We have demonstrated the feasibility of imaging mouse brain metabolism using photoacoustic computed tomography (PACT), a fast, noninvasive and functional imaging modality with optical contrast and acoustic resolution. Brain responses to forepaw stimulations were imaged transdermally and transcranially. 2-NBDG, which diffuses well across the blood-brain-barrier, provided exogenous contrast for photoacoustic imaging of glucose response. Concurrently, hemoglobin provided endogenous contrast for photoacoustic imaging of hemodynamic response. Glucose and hemodynamic responses were quantitatively decoupled by using two-wavelength measurements. We found that glucose uptake and blood perfusion around the somatosensory region of the contralateral hemisphere were both increased by stimulations, indicating elevated neuron activity. While the glucose response area was more homogenous and confined within the somatosensory region, the hemodynamic response area had a clear vascular pattern and spread wider than the somatosensory region. Our results demonstrate that 2-NBDG-enhanced PACT is a promising tool for noninvasive studies of brain metabolism.
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
authors.forename: Junjie
authors.initials: J
authors.lastname: Yao
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.forename: Konstantin I
authors.initials: KI
authors.lastname: Maslov
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Nasiriavanaki
authors.forename: Vassiliy
authors.initials: V
authors.lastname: Tsytsarev
authors.forename: Alexei V
authors.initials: AV
authors.lastname: Demchenko
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D000818:Animals; D003287:Contrast Media / Q000493:pharmacokinetics; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D054459:Elasticity Imaging Techniques / Q000379:methods*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D061088:Photoacoustic Techniques / Q000379:methods*; D013003:Somatosensory Cortex / Q000378:metabolism*; D014018:Tissue Distribution; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: D003287:Contrast Media; D003847:Deoxyglucose; D009327:4-Chloro-7-nitrobenzofurazan; D005947:Glucose; C098340:2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose