title: Injectable nanohybrid scaffold for biopharmaceuticals delivery and soft tissue engineering.
abstract: An injectable nanofibrous hydrogel scaffold integrated with growth factors (GFs) loaded polysaccharide nanoparticles was developed that specifically allows for targeted adipose-derived stem cells (ASCs) encapsulation and soft tissue engineering. The nanofibrous hydrogel was produced via biological conjugation of biotin-terminated star-shaped poly(ethylene glycol) (PEG-Biotin) and streptavidin-functionalized hyaluronic acid (HA-Streptavidin). The polysaccharide nanoparticles were noncovalently assembled via electrostatic interactions between low-molecular-weight heparin (LMWH) and N,N,N-trimethylchitosan chloride (TMC). Vascular endothelial growth factor (VEGF) was entrapped in the LMWH/TMC nanoparticles by affinity interactions with LMWH.
authors.affiliation: Department of Materials Science, School of Materials Science and Engineering, Nanjing University of Science and Technology, Nanjing 210094, China. hptan@njust.edu.cn
authors.forename: Huaping
authors.initials: H
authors.lastname: Tan
authors.forename: Qing
authors.initials: Q
authors.lastname: Shen
authors.forename: Xiaojia
authors.initials: X
authors.lastname: Jia
authors.forename: Zipeng
authors.initials: Z
authors.lastname: Yuan
authors.forename: Dangsheng
authors.initials: D
authors.lastname: Xiong
mesh_terms: D002214:Capsules; D048271:Chitosan / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D006495:Heparin, Low-Molecular-Weight / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D007267:Injections; D057139:Nanofibers / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D055672:Static Electricity; D013234:Stem Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*; D042461:Vascular Endothelial Growth Factor A / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D002214:Capsules; D004337:Drug Carriers; D006495:Heparin, Low-Molecular-Weight; D020100:Hydrogels; C118071:N-trimethyl chitosan chloride; D042461:Vascular Endothelial Growth Factor A; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D048271:Chitosan