title: Influence of surface coating of PLGA particles on the internalization and functions of human endothelial cells.
abstract: This study is focused on the uptake of PLGA particles with different coatings and its influences on the functions and toxicity of human endothelial cells. The PLGA particles coated with polyethyleneimine (PEI) or bovine serum albumin (BSA) were prepared via a one-step emulsion method, which had a similar diameter of ∼420 nm in water and ∼170 nm in a dry state but oppositely charged surfaces. Both types of the particles were readily internalized into cells within a short time regardless of their surface chemistry. Uptake of the positively charged particles caused apparently a decrease in cell viability, but did not significantly influence mitochondrial membrane potential and activity of caspase-3. The cell adhesion and migration were significantly affected, especially after uptake of the PLGA-PEI particles. The secretion levels of von Willebrand factor (vWF) and 6-k-PGF(1α) were not significantly influenced regardless of the surface coating.
authors.affiliation: Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Yu
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiangyan
authors.initials: X
authors.lastname: Zhou
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Mao
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004655:Emulsions / Q000737:chemistry; D004705:Endocytosis / Q000187:drug effects*; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D004655:Emulsions; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D011094:Polyethyleneimine