title: Analytical glycobiology at high sensitivity: current approaches and directions.
abstract: This review summarizes the analytical advances made during the last several years in the structural and quantitative determinations of glycoproteins in complex biological mixtures. The main analytical techniques used in the fields of glycomics and glycoproteomics involve different modes of mass spectrometry and their combinations with capillary separation methods such as microcolumn liquid chromatography and capillary electrophoresis. The need for high-sensitivity measurements have been emphasized in the oligosaccharide profiling used in the field of biomarker discovery through MALDI mass spectrometry. High-sensitivity profiling of both glycans and glycopeptides from biological fluids and tissue extracts has been aided significantly through lectin preconcentration and the uses of affinity chromatography.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington, IN, USA. novotny@indiana.edu
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
authors.forename: William R
authors.initials: WR
authors.lastname: Alley
authors.forename: Benjamin F
authors.initials: BF
authors.lastname: Mann
mesh_terms: D002846:Chromatography, Affinity; D054794:Glycomics / Q000639:trends; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D013058:Mass Spectrometry
chemical_list: D006023:Glycoproteins