title: [Identification of the differences in the genes responsible for methionine biosynthesis in Bacillus anthracis strains and phylogeny-related species].
abstract: The comparative analysis of the gene sequences encoding the synthesis of enzymes responsible for the intermediary metabolism of methionine in Bacillus anthracis strains and in closely related bacterial species was carried out. Deletion of 42 nucleotides in the hom2 gene, which determines the homoserinedehydrogenase, is detected in all tested Bacillus anthracis strains. In the strains of other bacillar species hom2 gene mutation, which blocks up the tracts of methionine and threonine biosynthesis, was not identified. The single nucleotide polymorphism was determined in asd1, metX, and metH genes. It provides the identification of B. anthracis strains using sequencing technology.
authors.forename: N I
authors.initials: NI
authors.lastname: Mikshis
authors.forename: Iu N
authors.initials: IuN
authors.lastname: Zhivova
authors.forename: L V
authors.initials: LV
authors.lastname: Novikova
authors.forename: N A
authors.initials: NA
authors.lastname: Sharapova
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Popov
authors.forename: V V
authors.initials: VV
authors.lastname: Kutyrev
mesh_terms: D001408:Bacillus anthracis / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D005798:Genes, Bacterial; D006715:Homoserine Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D054643:INDEL Mutation; D008715:Methionine / Q000096:biosynthesis / Q000235:genetics*; D010802:Phylogeny; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D008715:Methionine; D006715:Homoserine Dehydrogenase