title: A robust plate assay for detection of extracellular microbial protease activity in metagenomic screens and pure cultures.
abstract: A robust, efficient and cost-effective agar that utilises lactose free milk powder for identification of bacterial protease activity in pure cultures and metagenomic screens has been developed and tested on protease positive bacteria, selected strains and false protease positives isolated from a previously constructed metagenomic library.
authors.affiliation: Cardiff School of Biosciences, Cardiff University, Cardiff, CF10 3AT, UK.
authors.forename: Laura S
authors.initials: LS
authors.lastname: Morris
authors.forename: James
authors.initials: J
authors.lastname: Evans
authors.forename: Julian R
authors.initials: JR
authors.lastname: Marchesi
mesh_terms: D000362:Agar; D000818:Animals; D001419:Bacteria / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry*; D008892:Milk / Q000378:metabolism; D010447:Peptide Hydrolases / Q000032:analysis*
chemical_list: D003470:Culture Media; D000362:Agar; D010447:Peptide Hydrolases