title: Galectin 1 expression is associated with tumor invasion and metastasis in stage IB to IIA cervical cancer.
abstract: Galectin 1 is a 14-kd laminin-binding lectin involved in important biologic mechanisms of tumors, including neoplastic transformation, cell survival, angiogenesis, cell proliferation, and metastasis. In this study, we investigated the role of galectin 1 in cell survival and metastasis in cervical cancer. The expression of galectin 1 was determined in 73 formalin-fixed, paraffin-embedded cervical cancer tissues using an immunohistochemical method and compared with clinicopathologic risk factors for recurrence after surgery. To evaluate the role of galectin 1 in cell proliferation and invasion, we performed proliferation and invasion assays with galectin 1 small interfering RNA (siRNA) using cervical cancer cell lines, including HeLa and SiHa cells. Immunohistochemical analysis revealed that galectin 1 expression was found in most peritumoral stroma samples (72/73; 98.6%). Galectin 1 expression was significantly correlated with the depth of invasion in the cervix (P=.015) and lymph node metastasis (P=.045) on univariate analysis. When progression-free survival of all of the patients studied was analyzed based upon galectin 1 expression, galectin 1 expression was not correlated with progression-free survival (P=.32). Down-regulation of galectin 1 using small interfering RNA resulted in the inhibition of cell growth and proliferation of HeLa and SiHa cells. Moreover, the ability of cells to invade was significantly reduced by galectin 1 small interfering RNA. Our results revealed that high galectin 1 expression in peritumoral stroma was significantly correlated with depth of invasion in cervical lesions and lymph node metastasis of cervical cancer and that galectin 1 may be functionally involved in cell proliferation and invasion.
authors.affiliation: Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea.
authors.forename: Ha-Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: In-Gu
authors.initials: IG
authors.lastname: Do
authors.forename: Hye-Kyung
authors.initials: HK
authors.lastname: Jeon
authors.forename: Young Jae
authors.initials: YJ
authors.lastname: Cho
authors.forename: Young Ae
authors.initials: YA
authors.lastname: Park
authors.forename: Jung-Joo
authors.initials: JJ
authors.lastname: Choi
authors.forename: Chang Ohk
authors.initials: CO
authors.lastname: Sung
authors.forename: Yoo-Young
authors.initials: YY
authors.lastname: Lee
authors.forename: Chel Hun
authors.initials: CH
authors.lastname: Choi
authors.forename: Tae-Joong
authors.initials: TJ
authors.lastname: Kim
authors.forename: Byoung-Gie
authors.initials: BG
authors.lastname: Kim
authors.forename: Jeong-Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Duk-Soo
authors.initials: DS
authors.lastname: Bae
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology / Q000556:secondary; D018196:Carcinoma, Adenosquamous / Q000235:genetics / Q000473:pathology / Q000556:secondary; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology / Q000556:secondary; D045744:Cell Line, Tumor; D005260:Female; D037483:Galectin 1 / Q000096:biosynthesis* / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*
chemical_list: D037483:Galectin 1; C445499:LGALS1 protein, human