title: [The preanalytical stage of under measuring of concentration of catalytic activity of enzymes: the characteristics and tasks of standardization].
abstract: The article explains the importance of standardization, the development and maintenance of rules and recommendations regulating the conditions and order of implementation of particular parts of preanalytical stage. The order of these conditions is noted including the rules stated and published in such normative documents as national standards GOST R 15 189-2009, GOST R 53079.4-2008, GOST R ISO 6710-209 and in the recommendations of foreign National societies ofclini-cal chemistry and laboratory medicine. These requirements concern all the analytes, enzymes included. The cited data have a practical significance for acquisition of reliable results in everyday functioning of laboratories. Enough to mention data concerning the anticoagulants influence on catalytic concentration of enzymes, the most often measured concentrations of alpha-amylase, lipase, amynotransferase, alkaline and acid phosphatase, creatine kinase, lactate dehydrogenase, choline esterase, hemolysis impact, the increased concentration of bilirubin and hyperlipemia in samples and significance of measurement of indices of serum and plasma as well. The possible mechanisms of impact of these interferents on the results of measurement of catalytic concentration of enzymes are discussed.
authors.forename: T I
authors.initials: TI
authors.lastname: Lukicheva
authors.forename: V V
authors.initials: VV
authors.lastname: Men'shikov
mesh_terms: D002384:Catalysis; D002624:Chemistry, Clinical / Q000379:methods / Q000592:standards; D004796:Clinical Enzyme Tests / Q000379:methods / Q000592:standards; D004798:Enzymes / Q000097:blood* / Q000592:standards*; D006801:Humans; D012015:Reference Standards
chemical_list: D004798:Enzymes