title: [Detection of tissue factor-positive microparticles and its clinical significance in the haemostatic disorder].
abstract: Objective of this study was to detect the level of tissue factor-positive microparticles (TF(+)MP) by flow cytometry (FCM) and to analyze its clinical significance in the haemostatic disorder. TF(+) MP was detected by FCM using antibody CD142-PE in 25 cases of acute promyelocytic leukemia (APL), 20 cases of hemostatic diseases and 20 healthy adults as controls. The differences of TF(+) MP between various groups were determined. The results showed that the level of TF(+) MP in the patients with thrombotic complications was significantly higher than that in the healthy adults (P < 0.05). The TF(+) MP level was higher in the patient with APL than that in the healthy adults, especially in course before therapy (P < 0.01), but the difference was not statistically significant in the patient with APL after therapy and the healthy adults. Among these patient with APL, the level of TF(+) MP in the 18 patients who complicated with disseminated intravascular coagulation (DIC) was also higher than that in the healthy adults (P < 0.05), but the level of TF(+) MP in the other 7 patients who did not complicate with DIC was similar before and after treatment. It is concluded that the method of TF(+) MP detection by FCM is feasible and simple, it is useful for the diagnosis of thrombotic disorder, and helps evaluation for the prognosis of APL patient.
authors.affiliation: Department of Hematology, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhou
authors.forename: Yue
authors.initials: Y
authors.lastname: Han
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhu
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Hu
authors.forename: Shi-Xiang
authors.initials: SX
authors.lastname: Zhao
authors.forename: Ming-Qing
authors.initials: MQ
authors.lastname: Zhu
authors.forename: Lan
authors.initials: L
authors.lastname: Dai
authors.forename: Wen-Hong
authors.initials: WH
authors.lastname: Shen
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: De-Pei
authors.initials: DP
authors.lastname: Wu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001778:Blood Coagulation Disorders / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D013925:Thromboplastin / Q000378:metabolism*; D055815:Young Adult
chemical_list: D013925:Thromboplastin