title: Quantification of selected furocoumarins by high-performance liquid chromatography and UV-detection: capabilities and limits.
abstract: The performance of HPLC-UV as a means of quantifying selected furocoumarins in essential oils has been evaluated, based on a ring test validation approach. Accuracy profiles were generated, to determine bias and statistical confidence associated with determination at different concentrations, along with lower limits of quantification (LOQ). From these findings, it can be concluded that the method described may only be used in simple cases (essential oils), to measure individual furocoumarin compounds at concentrations greater than 10mg/l; the non compound-specific nature of detection by absorption in the UV range is unable to overcome the effect of interferences arising from chromatographic coelutions, such as those encountered in the analysis of complex commercial fragrance mixtures. The use of an algorithmically calculated 'spectral similarity' function, with reference to authentic standards, may be used to improve reliability in assignment and quantification.
authors.affiliation: Givaudan, Givaudan UK Ltd, Kennington Road, Ashford, Kent TN24 0LT, UK.
authors.forename: Angus P
authors.initials: AP
authors.lastname: Macmaster
authors.forename: Neil
authors.initials: N
authors.lastname: Owen
authors.forename: Sylvain
authors.initials: S
authors.lastname: Brussaux
authors.forename: Hugues
authors.initials: H
authors.lastname: Brevard
authors.forename: Richard
authors.initials: R
authors.lastname: Hiserodt
authors.forename: Hans
authors.initials: H
authors.lastname: Leijs
authors.forename: Nikola
authors.initials: N
authors.lastname: Bast
authors.forename: Berthold
authors.initials: B
authors.lastname: Weber
authors.forename: Gerd
authors.initials: G
authors.lastname: Loesing
authors.forename: Alan
authors.initials: A
authors.lastname: Sherlock
authors.forename: Christine
authors.initials: C
authors.lastname: Schippa
authors.forename: Matthias
authors.initials: M
authors.lastname: Vey
authors.forename: Eric
authors.initials: E
authors.lastname: Frérot
authors.forename: Emeline
authors.initials: E
authors.lastname: Tissot
authors.forename: Alain
authors.initials: A
authors.lastname: Chaintreau
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D045424:Complex Mixtures / Q000737:chemistry; D011564:Furocoumarins / Q000032:analysis*; D009822:Oils, Volatile / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D045424:Complex Mixtures; D011564:Furocoumarins; D009822:Oils, Volatile