title: Immobilization of pectinase from Leucoagaricus gongylophorus on magnetic particles.
abstract: Polygalacturonases (EC 3.2.1.15) hydrolyze the α-1,4-glycosidic linkages in polygalacturonic acid chains. The interest on specific inhibitors of pectinase and the versatility of magnetic support for enzyme immobilization endorsed the preparation of an immobilized enzyme reactor (IMER). This work presents the synthesis of CoFe(2)O(4) amino-derivatives, which was employed as the support for the immobilization of pectinases from Leucoagaricus gongylophorus. Amino-functionalized CoFe(2)O(4) was obtained from glyceryl-derivatized CoFe(2)O(4) and was characterized by infrared spectroscopy and electronic microscopy. The immobilized enzyme maintained the same thermal, chemical and kinetic behaviour of the free enzyme (T(opt) 60 °C; pH(opt) 5.0; K(app)(M) = 0.5 mg min(-1); V(app)(M) ≈ 5.0 μmol min(-1) mL(-1)). The straightforward synthesis of CoFe(2)O(4) derivatives and the efficiency of immobilization offer wide perspectives for the use of the developed new IMER.
authors.affiliation: Departamento de Química, Universidade Federal de São Carlos, São Paulo, Brazil.
authors.forename: Paulo Roberto
authors.initials: PR
authors.lastname: Adalberto
authors.forename: Francisco
authors.initials: F
authors.lastname: José dos Santos
authors.forename: Camilla Calemi
authors.initials: CC
authors.lastname: Golfeto
authors.forename: Mônica Rosas
authors.initials: MR
authors.lastname: Costa Iemma
authors.forename: Dulce Helena
authors.initials: DH
authors.lastname: Ferreira de Souza
authors.forename: Quezia Bezerra
authors.initials: QB
authors.lastname: Cass
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D011096:Polygalacturonase / Q000737:chemistry / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013816:Thermodynamics
chemical_list: D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C001215:ferrite; D011096:Polygalacturonase