title: Fluorescence determination of azithromycin in pharmaceutical formulations by using the synchronous scanning approach after its acid derivatization.
abstract: In this present work, a fluorescence method for azithromycin (9-deoxo-9a-aza-9a-methyl-9a-homoerythromycin) determination in pharmaceutical formulations is proposed. The method is based on the synchronous fluorescence (Δλ = 30 nm, 482 nm) produced when azithromycin is derivatized in strong acidic medium (9.0 mol L(-1) HCl). The influence of the derivatization conditions (acid concentration, reaction time and temperature) was studied. Also, the possible reaction mechanism was discussed. In the optimized conditions, the method presented a limit of detection of 0.23 mg L(-1) and a limit of quantification of 0.76 mg L(-1). The developed procedure was successfully applied in the determination of azithromycin in pharmaceutical formulations.
authors.affiliation: Departamento de Química Analítica, Universidade Federal Fluminense, Outeiro de São João Batista s/n, Centro, Niterói, RJ 24020-141, Brazil.
authors.forename: Vanessa G K
authors.initials: VG
authors.lastname: Almeida
authors.forename: Victor S M
authors.initials: VS
authors.lastname: Braga
authors.forename: Wagner F
authors.initials: WF
authors.lastname: Pacheco
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Cassella
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry*; D017963:Azithromycin / Q000032:analysis* / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D006851:Hydrochloric Acid / Q000737:chemistry*; D007700:Kinetics; D057230:Limit of Detection; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D004364:Pharmaceutical Preparations; D017963:Azithromycin; D006851:Hydrochloric Acid