title: [Effects of Bmi-1 RNAi gene on laryngeal carcinoma Hep-2 cells].
abstract: OBJECTIVE
  To construct the Bmi-1 RNAi expression vector and investigate its influence on the proliferation and invasiveness of laryngeal carcinoma Hep-2 cells.
  
  
  METHODS
  The recombinant vshRNA-Bmi-1 plasmid of Bmi-1 RNAi was constructed by the lentiviral expression system, pHelper1.0/pHelper2.0/pGCL2GFP. Bmi-1mRNA and protein expressions of stably transfected laryngeal carcinoma cells were identified tespectively by real-time PCR and Western blot analyses. The changes of the proliferation and invasiveness of laryngeal carcinoma were detected by clone formation test and an invasion assay.
  
  
  RESULTS
  The Bmi-1 mRNA expressions of stably transfected laryngeal carcinoma Hep-2 cells were significantly decreased. The expression of Bmi-1 protein in laryngeal carcinoma Hep-2 cells was significantly decreased. And the inhibitory rates were 79% and 88% respectively. Whereas the proliferation and invasiveness of Hep-2 cells were significantly reduced.
  
  
  CONCLUSIONS
  The Bmi-1 RNAi expression vector were constructed successful. Reduced Bmi-1 expression of Hep-2 cells demonstrated the role of Bmi-1 RNAi in restraining proliferation and invasiveness of laryngeal carcinoma cells.
authors.affiliation: Department of Otolaryngology, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, 710061, China. yaoxiaobao@sohu.com
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Yao
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.forename: Shaoqiang
authors.initials: S
authors.lastname: Zhang
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zhu
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000235:genetics; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism*; D014162:Transfection
chemical_list: C069077:BMI1 protein, human; D034741:RNA, Small Interfering; D063150:Polycomb Repressive Complex 1