title: Highly sensitive luminol electrochemiluminescence immunosensor based on ZnO nanoparticles and glucose oxidase decorated graphene for cancer biomarker detection.
abstract: In this work, we reported a sandwiched luminol electrochemiluminescence (ECL) immunosensor using ZnO nanoparticles (ZnONPs) and glucose oxidase (GOD) decorated graphene as labels and in situ generated hydrogen peroxide as coreactant. In order to construct the base of the immunosensor, a hybrid architecture of Au nanoparticles and graphene by reduction of HAuCl(4) and graphene oxide (GO) with ascorbic acid was prepared. The resulted hybrid architecture modified electrode provided an excellent platform for immobilization of antibody with good bioactivity and stability. Then, ZnONPs and GOD functionalized graphene labeled secondary antibody was designed for fabricating a novel sandwiched ECL immunosensor. Enhanced sensitivity was obtained by in situ generating hydrogen peroxide with glucose oxidase and the catalysis of ZnONPs to the ECL reaction of luminol-H(2)O(2) system. The as-prepared ECL immunosensor exhibited excellent analytical property for the detection of carcinoembryonic antigen (CEA) in the range from 10 pg mL(-1) to 80 ng mL(-1) and with a detection limit of 3.3 pg mL(-1) (SN(-1)=3). The amplification strategy performed good promise for clinical application of screening of cancer biomarkers.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, People's Republic of China.
authors.forename: Yinfeng
authors.initials: Y
authors.lastname: Cheng
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Huan
authors.initials: H
authors.lastname: Niu
authors.forename: Yaling
authors.initials: Y
authors.lastname: Cao
authors.forename: Huijing
authors.initials: H
authors.lastname: Liu
authors.forename: Lijuan
authors.initials: L
authors.lastname: Bai
authors.forename: Yali
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques; D002272:Carcinoembryonic Antigen / Q000097:blood; D055664:Electrochemical Techniques; D004566:Electrodes; D005949:Glucose Oxidase / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods; D008165:Luminol / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D009369:Neoplasms / Q000097:blood*; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D008165:Luminol; D006046:Gold; D006108:Graphite; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D001205:Ascorbic Acid; D015034:Zinc Oxide