title: Applicability of 2-DE to assess differences in the protein profile between cold storage and not cold storage in nectarine fruits.
abstract: Cold storage is being used to increase nectarine fruits' postharvest life. However, low temperatures lead to chilling injury and limit their commercial quality and value. In this study a proteomic approach was used to compare the protein profile between control and cold storage nectarine fruits. Protein extracted from tissue was resolved by two-dimensional gel electrophoresis (2-DE) in the 4-7 pH and 10-200 kDa Mr range. Around 350 spots were well determined, and 11 from 17 spots that showed significant differences were identified by peptide mass fingerprinting (PMF) using matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-mass spectrometry (MS). Four differentially expressed proteins were characterized as allergens and were further assessed at the transcription level using quantitative real time-RT-PCR (qRT-PCR).
authors.affiliation: Proteomics Laboratory, Instituto Tecnológico Agroalimentario de Extremadura (INTAEX), Gobierno de Extremadura, Av/Adolfo Suárez, s/n Aptdo. 20107, C.P. 06071 Badajoz, Spain. esther.giraldor@juntaextremadura.es
authors.forename: E
authors.initials: E
authors.lastname: Giraldo
authors.forename: A
authors.initials: A
authors.lastname: Díaz
authors.forename: J M
authors.initials: JM
authors.lastname: Corral
authors.forename: A
authors.initials: A
authors.lastname: García
mesh_terms: D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D061353:Food Storage / Q000379:methods; D005638:Fruit / Q000737:chemistry; D010449:Peptide Mapping; D010940:Plant Proteins / Q000032:analysis*; D027861:Prunus; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins