title: [Effects of paclitaxel on proliferation and transition of phenotype in platelet-derived growth factor-BB-induced pulmonary vascular smooth muscle cells].
abstract: OBJECTIVE
  To investigate the effects of paclitaxel on the phenotypic modulation induced by platelet-derived growth factor (PDGF-BB) in rat pulmonary vascular smooth muscle cells (PVSMC).
  
  
  METHODS
  The proliferation of PVSMC isolated from SD rats cultured in vitro was induced by PDGF-BB and then intervened by different concentration of paclitaxel. MTT and [³H]-thymidine incorporation were used to detect the changes of cell proliferation. The expression level of alpha-smooth muscle-actin (SM-α-actin) and smooth muscle protein 22alpha (SM22α) were tested by Western blot. Confocal laser scanning microscopy was applied to observe the change of fluorescence intensity.
  
  
  RESULTS
  Treatment with PDGF-BB for 24 hours results in a significant increase in [³H]-thymidine incorporation and marked change in phenotype and cytoskeleton, Paclitaxel inhibited the proliferation of PVSMC induced by PDGF-BB, the inhibition rate was 45.4%, 35.4%, 21.6% (P < 0.01) tested by[³H]-thymidine incorporation and 40.0%, 30.0%, 18.0% (P < 0.01) tested by MTT. Meanwhile, the paclitaxel promoted the expression level of SM-α-actin and SM22α. Fluorescence intensity of F-actin decreased significantly.
  
  
  CONCLUSIONS
  Paclitaxel may play an important role in vascular remodeling by changing the phenotypes and cytoskeleton of VSMC stimulated by PDGF-BB.
authors.affiliation: West China Institute of Women and Children's Health, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xiao-shuang
authors.initials: XS
authors.lastname: Wang
authors.forename: Zhi-yu
authors.initials: ZY
authors.lastname: Fan
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Xie
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.forename: Zheng-xiang
authors.initials: ZX
authors.lastname: Gao
authors.forename: Han-min
authors.initials: HM
authors.lastname: Liu
mesh_terms: D000818:Animals; D000077214:Becaplermin; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects; D008168:Lung / Q000098:blood supply; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects*; D017239:Paclitaxel / Q000494:pharmacology*; D010641:Phenotype; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D017239:Paclitaxel