title: Hepatoprotective effects of Berberis vulgaris L. extract/β cyclodextrin on carbon tetrachloride-induced acute toxicity in mice.
abstract: The present study investigated the capacity of formulated Berberis vulgaris extract/β-cyclodextrin to protect liver against CCl(4)-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl(4) on the 8th day. After 24 h of CCl(4) administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl(4) was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/β-cyclodextrin treatment prevents hepatic injury induced by CCl(4) and can be considered for further nutraceutical studies.
authors.affiliation: Department of Histology, Faculty of Medicine, Pharmacy and Dentistry, Vasile Goldis Western University of Arad, 1 Feleacului, Arad 310396, Romania.|Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, Arad 310414, Romania.
authors.forename: Anca
authors.initials: A
authors.lastname: Hermenean
authors.affiliation: Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, Arad 310414, Romania.
authors.forename: Cristina
authors.initials: C
authors.lastname: Popescu
authors.affiliation: Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, Arad 310414, Romania.
authors.forename: Aurel
authors.initials: A
authors.lastname: Ardelean
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Miruna
authors.identifier: 0000-0002-6273-8540
authors.initials: M
authors.lastname: Stan
authors.affiliation: Food Quality Department, Faculty of Food Processing Technology, Banat's University of Agricultural Sciences and Veterinary Medicine, 119 Calea Aradului, Timisoara 300645, Romania.
authors.forename: Nicoleta
authors.initials: N
authors.lastname: Hadaruga
authors.affiliation: Department of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, Arad 310414, Romania.
authors.forename: Ciprian-Valentin
authors.initials: CV
authors.lastname: Mihali
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Marieta
authors.initials: M
authors.lastname: Costache
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D028142:Berberis / Q000737:chemistry*; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D010088:Oxidoreductases / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D047392:beta-Cyclodextrins; D008315:Malondialdehyde; D010088:Oxidoreductases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase