title: Effects of antibodies to EG-VEGF on angiogenesis in the chick embryo chorioallantoic membrane.
abstract: BACKGROUND
  Endocrine gland-related vascular endothelial growth factor (EG-VEGF), is an angiogenic factor specifically targeting endothelial cells derived from endocrine tissues. The inhibition of the EG-VEGF/prokineticin receptor pathway could represent a selective antiangiogenic and anticancer strategy.
  
  
  OBJECTIVE
  to evaluate the impact of an antibody to EG-VEGF on the rapidly growing capillary plexus of the chick embryo chorioallantoic membrane (CAM).
  
  
  METHODS
  The in ovo CAM assay was performed for the humanized EG-VEGF antibody.
  
  
  RESULTS
  Hemorrhagic damage was induced in the capillaries, which led to early death of the embryos. Upon morphological staining, there was evidence of vascular disruption and extravasation of red blood cells in the chorion. Signs of vacuolization of the covering epithelium were also observed.
  
  
  CONCLUSIONS
  Blocking endogenous EG-VEGF might represent a valuable approach of impairing or inhibiting angiogenesis in steroidogenic-derived embryonic tissues.
authors.affiliation: Department of Histology, Angiogenesis Research Center, Victor Babes University of Medicine and Pharmacy Timisoara, Timis, Romania.
authors.forename: Stefana
authors.initials: S
authors.lastname: Feflea
authors.forename: Anca Maria
authors.initials: AM
authors.lastname: Cimpean
authors.forename: Raluca Amalia
authors.initials: RA
authors.lastname: Ceausu
authors.forename: Pusa
authors.initials: P
authors.lastname: Gaje
authors.forename: Marius
authors.initials: M
authors.lastname: Raica
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D030161:Avian Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply* / Q000166:cytology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D018919:Neovascularization, Physiologic; D014617:Vacuoles / Q000187:drug effects; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000378:metabolism; D042662:Vascular Endothelial Growth Factor, Endocrine-Gland-Derived / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D030161:Avian Proteins; D042662:Vascular Endothelial Growth Factor, Endocrine-Gland-Derived; D040321:Vascular Endothelial Growth Factor Receptor-3