title: Hybrid nanoparticle architecture for cellular uptake and bioimaging: direct crystallization of a polymer immobilized with magnetic nanoparticles on carbon nanotubes.
abstract: We describe here the success of an innovative approach of direct immobilization of magnetic nanoparticles (MNPs) onto carbon nanotubes (CNTs). The approach involved functionalization of magnetic nanoparticles and consequent covalent linkage to a copolymer (PE-b-PEG). Next, the immobilized magnetic nanoparticles on the copolymer were directly crystallized on the long axis of CNTs, where the interfacial adhesion comes from electrostatic and van der Waals interaction. The intracellular trafficking of a hybrid nanoparticle system [(PE-b-PEG)-MNP-CNT-FITC] in HeLa cells was monitored using a fluorescent marker, FITC, conjugated to the nanoparticle system. The distribution of the nanoparticle system inside cells was studied by fluorescence microscopy in a time and dose dependent manner, and it was observed that the nanoparticles are located in the cytoplasm and no apparent cell death was observed at the concentration studied. Also, the effect of an externally applied magnetic field on actin cytoskeleton, cell morphology and intracellular uptake of iron was studied. The approach described here is promising for simultaneous imaging and monitoring intracellular uptake.
authors.affiliation: Biomaterials and Biomedical Engineering Research Laboratory, Center for Structural and Functional Materials, University of Louisiana at Lafayette, P.O. Box 44130, Lafayette, LA 70504-4130, USA.
authors.forename: D
authors.initials: D
authors.lastname: Depan
authors.forename: R D K
authors.initials: RD
authors.lastname: Misra
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D003460:Crystallization; D004574:Electromagnetic Fields; D006367:HeLa Cells; D006801:Humans; D007501:Iron / Q000493:pharmacokinetics; D007700:Kinetics; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D020959:Polyethylene / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D022502:Stress Fibers / Q000528:radiation effects
chemical_list: D058185:Magnetite Nanoparticles; D037742:Nanotubes, Carbon; D011092:Polyethylene Glycols; D020959:Polyethylene; D007501:Iron