title: Sequence and comparative analysis of the MIP gene in Chinese straw mushroom, Volvariella volvacea.
abstract: The mitochondrial intermediate peptidase (MIP) gene is conserved in fungi. It is linked closely with the mating-type A (mtA) gene. In this study, a fragment of the MIP gene in  Volvariella volvacea  (Bull. ex Fr.) Singer was first cloned by homologue-based cloning technology. Subsequently, the entire MIP DNA sequence (PYd21-MIP) was obtained after the fragment was compared with the genomic data through BLAST analysis. The PYd21-MIP sequence appeared to be homologous with the MIP gene in other fungi. Phylogenetic analysis of PYd21-MIP and other MIP sequences from diverse fungi agreed with the current organism phylogeny. Analysis of protein domains by InterProScan software and motif searching demonstrated that PYd21-MIP encodes a homologous MIP protein. These data support the hypothesis that the PYd21-MIP protein is a Hog-MIP protein homologue from V. volvacea.
authors.affiliation: Mycological Research Center, Fujian Agricultural and Forestry University, Fuzhou, China.
authors.forename: Bing-Zhi
authors.initials: BZ
authors.lastname: Chen
authors.forename: Fu
authors.initials: F
authors.lastname: Gui
authors.forename: Bao-Gui
authors.initials: BG
authors.lastname: Xie
authors.forename: Feng
authors.initials: F
authors.lastname: Zou
authors.forename: Yu-Ji
authors.initials: YJ
authors.lastname: Jiang
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Deng
mesh_terms: D002681:China; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D049770:Genes, Mating Type, Fungal; D008666:Metalloendopeptidases / Q000235:genetics*; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D055367:Volvariella / Q000737:chemistry / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D008666:Metalloendopeptidases; C068038:mitochondrial intermediate peptidase