title: Optimization of the hydrogen yield from single-stage photofermentation of glucose by Rhodobacter capsulatus JP91 using response surface methodology.
abstract: Hydrogen production from glucose via single-stage photofermentation was examined with the photosynthetic bacterium Rhodobacter capsulatus JP91 (hup-). Response surface methodology with Box-Behnken design was used to optimize the independent experimental variables of glucose concentration, glutamate concentration and light intensity, as well as examining their interactive effects for maximization of molar hydrogen yield. Under optimal condition with a light intensity of 175W/m(2), 35mM glucose, and 4.5mM glutamate, a maximum hydrogen yield of 5.5 (±0.15)molH(2)/molglucose, and a maximum nitrogenase activity of 246 (±3.5)nmolC(2)H(4)/ml/min were obtained. Densitometric analysis of nitrogenase Fe-protein expression under different conditions showed significant variation in Fe-protein expression with a maximum at the optimized central point. Even under optimum conditions for hydrogen production, a significant fraction of the Fe-protein was found in the ADP-ribosylated state, suggesting that further improvement in yields might be possible.
authors.affiliation: Département de Microbiologie et Immunologie, Université de Montréal, CP 6128 Succursale Centre-ville, Montréal, Québec, Canada H3C 3J7.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Ghosh
authors.forename: Irma Flore
authors.initials: IF
authors.lastname: Sobro
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Hallenbeck
mesh_terms: D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D003720:Densitometry; D005285:Fermentation / Q000528:radiation effects*; D005947:Glucose / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D008027:Light; D009591:Nitrogenase / Q000378:metabolism; D016354:Rhodobacter capsulatus / Q000201:enzymology / Q000378:metabolism* / Q000528:radiation effects*; D013379:Substrate Specificity / Q000528:radiation effects
chemical_list: D006859:Hydrogen; D009591:Nitrogenase; D005947:Glucose