title: Multifunctionalized cantilever systems for electronic nose applications.
abstract: Multiple target detection using a cantilever is essential for biosensor, chemical sensor, and electronic nose systems. We report a novel microcantilever array chip that includes four microreaction chambers in a chip, which consequently contains four different functionalized surfaces for multitarget detection. For model tests, we designed microcantilever chips and demonstrated the ability of binding of 2,4-dinitrotoluene (DNT) targets onto four different surfaces. We used peptide receptors that are known to have highly selective binding. By simply using four microreaction chambers, we immobilized DNT specific peptide (HPNFSKYILHQRC; SP), DNT nonspecific peptide (TSMLLMSPKHQAC; NSP), and self-assembled monolayer (SAM) as well as a bare cantilever. After flowing DNT gases through the cantilever chip, we could monitor the four different binding signals simultaneously. The shifts in NSP provided information as a negative control because it contained information of temperature fluctuations and mechanical vibration from gas flow. By utilizing the differential signal of the SP and NSP, we acquired 7.5 Hz in resonant responses that corresponds with 160 part per billion (ppb) DNT concentration, showing the exact binding response by eliminating the inevitable thermal noise, vibration noise, as well as humidity effects on the peptide surface.
authors.affiliation: Department of Electrical Engineering, Kwangwoon University, 447-1, Wolgye, Nowon, Seoul, 139-701, Korea.
authors.forename: Yong Kyoung
authors.initials: YK
authors.lastname: Yoo
authors.forename: Myung-Sic
authors.initials: MS
authors.lastname: Chae
authors.forename: Ji Yoon
authors.initials: JY
authors.lastname: Kang
authors.forename: Tae Song
authors.initials: TS
authors.lastname: Kim
authors.forename: Kyo Seon
authors.initials: KS
authors.lastname: Hwang
authors.forename: Jeong Hoon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D004136:Dinitrobenzenes / Q000032:analysis*; D004581:Electronics / Q000295:instrumentation; D006813:Humidity; D009666:Nose; D009842:Oligopeptides / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D014732:Vibration
chemical_list: D004136:Dinitrobenzenes; D009842:Oligopeptides; C016403:2,4-dinitrotoluene