title: New biofuel integrating glycerol into its composition through the use of covalent immobilized pig pancreatic lipase.
abstract: By using 1,3-specific Pig Pancreatic lipase (EC 3.1.1.3 or PPL), covalently immobilized on AlPO(4)/Sepiolite support as biocatalyst, a new second-generation biodiesel was obtained in the transesterification reaction of sunflower oil with ethanol and other alcohols of low molecular weight. The resulting biofuel is composed of fatty acid ethyl esters and monoglycerides (FAEE/MG) blended in a molar relation 2/1. This novel product, which integrates glycerol as monoacylglycerols (MG) into the biofuel composition, has similar physicochemical properties compared to those of conventional biodiesel and also avoids the removal step of this by-product. The biocatalyst was found to be strongly fixed to the inorganic support (75%). Nevertheless, the efficiency of the immobilized enzyme was reduced to half (49.1%) compared to that of the free PPL. The immobilized enzyme showed a remarkable stability as well as a great reusability (more than 40 successive reuses) without a significant loss of its initial catalytic activity. Immobilized and free enzymes exhibited different reaction mechanisms, according to the different results in the Arrhenius parameters (Ln A and Ea). However, the use of supported PPL was found to be very suitable for the repetitive production of biofuel due to its facile recyclability from the reaction mixture.
authors.affiliation: Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014, Córdoba, Spain.
authors.forename: Diego
authors.identifier: 0000-0003-1478-7476
authors.initials: D
authors.lastname: Luna
authors.affiliation: Seneca Green Catalyst S.L., Campus de Rabanales, 14014, Córdoba, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Posadillo
authors.affiliation: Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014, Córdoba, Spain.
authors.forename: Verónica
authors.initials: V
authors.lastname: Caballero
authors.affiliation: Crystallographic Studies Laboratory, Andalusian Institute of Earth Sciences, CSIC, Avda. Las Palmeras n°4, 18100, Armilla, Granada, Spain.
authors.forename: Cristóbal
authors.initials: C
authors.lastname: Verdugo
authors.affiliation: Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014, Córdoba, Spain.
authors.forename: Felipa M
authors.initials: FM
authors.lastname: Bautista
authors.affiliation: Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014, Córdoba, Spain.
authors.forename: Antonio A
authors.initials: AA
authors.lastname: Romero
authors.affiliation: Department of Microbiology, University of Córdoba, Campus de Rabanales, Ed. Marie Curie, 14014, Córdoba, Spain.
authors.forename: Enrique D
authors.initials: ED
authors.lastname: Sancho
authors.affiliation: Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014, Córdoba, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Luna
authors.affiliation: Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014, Córdoba, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Calero
mesh_terms: D000438:Alcohols / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D056804:Biofuels; D002849:Chromatography, Gas; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D004951:Esterification; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005990:Glycerol / Q000737:chemistry / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry / Q000378:metabolism; D000074242:Sunflower Oil; D013552:Swine; D013696:Temperature; D014783:Viscosity
chemical_list: D000438:Alcohols; D056804:Biofuels; D004800:Enzymes, Immobilized; D005227:Fatty Acids; D010938:Plant Oils; D000074242:Sunflower Oil; D008049:Lipase; D005990:Glycerol