title: Conditional Aurora A deficiency differentially affects early mouse embryo patterning.
abstract: Aurora A is a mitotic kinase essential for cell proliferation. In mice, ablation of Aurora A results in mitotic arrest and pre-implantation lethality, preventing studies at later stages of development. Here we report the effects of Aurora A ablation on embryo patterning at early post-implantation stages. Inactivation of Aurora A in the epiblast or visceral endoderm layers of the conceptus leads to apoptosis and inhibition of embryo growth, causing lethality and resorption at approximately E9.5. The effects on embryo patterning, however, depend on the tissue affected by the mutation. Embryos with an epiblast ablation of Aurora A properly establish the anteroposterior axis but fail to progress through gastrulation. In contrast, mutation of Aurora A in the visceral endoderm, leads to posteriorization of the conceptus or failure to elongate the anteroposterior axis. Injection of ES cells into Aurora A epiblast knockout blastocysts reconstitutes embryonic development to E9.5, indicating that the extra-embryonic tissues in these mutant embryos can sustain development to organogenesis stages. Our results reveal new ways to induce apoptosis and to ablate cells in a tissue-specific manner in vivo. Moreover, they show that epiblast-ablated embryos can be used to test the potency of stem cells.
authors.affiliation: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
authors.forename: Yeonsoo
authors.initials: Y
authors.lastname: Yoon
authors.forename: Dale O
authors.initials: DO
authors.lastname: Cowley
authors.forename: Judith
authors.initials: J
authors.lastname: Gallant
authors.forename: Stephen N
authors.initials: SN
authors.lastname: Jones
authors.forename: Terry
authors.initials: T
authors.lastname: Van Dyke
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Rivera-Pérez
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D064096:Aurora Kinase A; D064127:Aurora Kinases; D019521:Body Patterning / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004622:Embryo, Mammalian / Q000196:embryology*; D053595:Embryonic Stem Cells / Q000378:metabolism; D004707:Endoderm / Q000196:embryology*; D005455:Fluorescent Antibody Technique; D055786:Gene Knockout Techniques; D005855:Germ Layers / Q000196:embryology*; D017403:In Situ Hybridization; D051379:Mice; D017346:Protein Serine-Threonine Kinases / Q000172:deficiency* / Q000235:genetics; D001616:beta-Galactosidase
chemical_list: D017931:DNA Primers; C576918:Aurka protein, mouse; D064096:Aurora Kinase A; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; D001616:beta-Galactosidase