title: New informatics and automated infrastructure to accelerate new leads discovery by high throughput screening (HTS).
abstract: The Lankenau Institute for Medical Research Chemical Genomics Center, Inc. has developed a new (patents issued and pending) Nanotube Automated Repository System (NARS) for dynamic storage of millions of 'single-shot' samples stored in a new monolithic microtiter-storage tube plate of our own design we call 'nanotubes.' We have integrated the NARS with customized software to efficiently access up to 10,000,000 samples stored continuously frozen (-20°C) in a dehumidified enclosure and sealed in a new microtiter NARS plate that is SBS compliant. Additional software was developed to analyze HTS data from orthogonally pooled compound libraries. Following 'de-convolution' of pooled HTS data, the software designates confirmatory retest samples to be 'cherry-picked' using the NARS. The application of a new, fully-integrated infrastructure for new leads discovery is described in detail. Other applications for our technologies and new infrastructure are discussed.
authors.affiliation: Lankenau Institute for Medical Research Chemical Genomics Center-LCGC, Inc., 100 Lancaster Avenue, Wynnewood, PA 19096, USA. donoverp@mlhs.org
authors.forename: Preston S
authors.initials: PS
authors.lastname: Donover
authors.forename: Marlin
authors.initials: M
authors.lastname: Yohn
authors.forename: Matthew
authors.initials: M
authors.lastname: Sim
authors.forename: Andrew
authors.initials: A
authors.lastname: Wright
authors.forename: Sandesh
authors.initials: S
authors.lastname: Gowda
authors.forename: Chip
authors.initials: C
authors.lastname: Allee
authors.forename: Amanda R
authors.initials: AR
authors.lastname: Schabdach
authors.forename: Melvin
authors.initials: M
authors.lastname: Reichman
mesh_terms: D062313:Databases, Pharmaceutical; D055808:Drug Discovery / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D012984:Software