title: In vitro protective effects of Thymus quinquecostatus Celak extracts on t-BHP-induced cell damage through antioxidant activity.
abstract: The purpose of this study was to evaluate the antioxidative activities of water and 70% ethanolic extracts from the Thymus quinquecostatus Celak (TQC) for natural antioxidant source. The antioxidant activities were compared with other natural and synthetic antioxidants. The levels of total polyphenols and flavonoids were also determined. The extracts were found to have different levels of antioxidant properties in a few kind of assay. The results showed that higher radical scavenging activity, reducing power and antioxidant capacity in FRAP than those of BHT as a positive control. In addition, the extracts from the TQC leaf and stem showed stronger antioxidant activity than that of vitamin C, α-tocopherol in ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. Cytoprotective and anti-apoptotic effect of water extracts from TQC was also prevented t-BHP-induced toxicity in Chang liver cells. Therefore, these results indicate that TQC extracts have antioxidant properties through its ability to enhance the cell viability, reduction of production of ROS, inhibition of oxidative damage, mitochondria dysfunction and ultimately inhibition of cell apoptosis. Based on the results described above, it is suggested that TQC has the potential to protect liver on t-BHP-induced cell damage and should be considered as a prospective functional food.
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju, Chungbuk 380-701, Republic of Korea.
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Seong-Eun
authors.initials: SE
authors.lastname: Kim
authors.forename: Ee-Hwa
authors.initials: EH
authors.lastname: Kim
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.forename: Byung-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D052160:Benzothiazoles; D001713:Biphenyl Compounds / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D007501:Iron / Q000737:chemistry / Q000378:metabolism; D019787:Linoleic Acid / Q000737:chemistry; D015227:Lipid Peroxidation / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D013451:Sulfonic Acids / Q000737:chemistry / Q000378:metabolism; D013844:Thiazoles / Q000737:chemistry / Q000378:metabolism; D013861:Thiocyanates / Q000737:chemistry / Q000378:metabolism; D046930:Thymus Plant / Q000737:chemistry*; D024502:alpha-Tocopherol / Q000494:pharmacology; D020122:tert-Butylhydroperoxide / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D010851:Picrates; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species; D013451:Sulfonic Acids; D013844:Thiazoles; D013861:Thiocyanates; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C103724:ferric thiocyanate; D020122:tert-Butylhydroperoxide; D019787:Linoleic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D007501:Iron; D024502:alpha-Tocopherol