title: Aquaporin changes in compound 48/80 induced inflammatory sublaryngeal edema in rat.
abstract: OBJECTIVE
  Laryngeal edema is a common clinical condition. However, the underlying molecular mechanisms remain elusive. Aquaporins (AQPs) are small integral plasma membrane proteins that transport water across the plasma membrane. In this study, we explore the relationship between inflammatory laryngeal edema induced by compound 48/80 and the expression of AQPs.
  
  
  METHODS
  Prospective, controlled, experimental animal study.
  
  
  METHODS
  Healthy adult male SD rats were injected with either sterile water, compound 48/80 (2 mg/kg), or compound 48/80 plus dexamethasone (3 mg/kg) via the tail vein. The larynxes were harvested 10, 30 minutes, and 1 hour after the injection for the measurement of sublaryngeal water content and histological and molecular evaluations.
  
  
  RESULTS
  Ten and 30 minutes after the compound 48/80 injection compared with the sterile water injection control groups, the water content in subglottic larynx increased significantly and the tissues were markedly swollen accompanied with inflammatory cell infiltration. AQP1 and AQP5 mRNA decreased significantly. One hour after the compound 48/80 injection, the edema was diminished, but the inflammatory cell infiltration remained. AQP1 was elevated but AQP5 was still lower than controls. Dexamethasone did not significantly reduce laryngeal edema, but significantly reduced inflammatory cells infiltration induced by compound 48/80 injection. Dexamethasone increased the AQP5 level but not AQP1.
  
  
  CONCLUSIONS
  AQP1 and AQP5 might play key roles in inflammatory subglottic edema caused by compound 48/80 in rats. AQP1 and AQP5 might be useful molecular targets of clinical treatment of inflammatory laryngeal edema.
authors.affiliation: Department of Anatomy, China Medical University, Shenyang, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Liu
authors.forename: Huanjiu
authors.initials: H
authors.lastname: Xi
authors.forename: Weiwei
authors.initials: W
authors.lastname: Xing
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D051398:Aquaporin 1 / Q000235:genetics / Q000378:metabolism*; D051400:Aquaporin 3 / Q000378:metabolism; D051401:Aquaporin 4 / Q000378:metabolism; D051402:Aquaporin 5 / Q000235:genetics / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000517:prevention & control; D005786:Gene Expression Regulation; D007827:Laryngitis / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000517:prevention & control; D007830:Larynx / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D000893:Anti-Inflammatory Agents; C496022:Aqp1 protein, rat; C496029:Aqp3 protein, rat; C496032:Aqp4 protein, rat; C496040:Aqp5 protein, rat; D051401:Aquaporin 4; D051402:Aquaporin 5; D012333:RNA, Messenger; D051398:Aquaporin 1; D051400:Aquaporin 3; D003189:p-Methoxy-N-methylphenethylamine; D003907:Dexamethasone