title: [Effect of bleomycin A5 on the hemangioma-derived endothelial cell line XTPS-1].
abstract: OBJECTIVE
  To investigate the effect of bleomycin A5 on the hemangioma-derived endothelial cell line XTPS-1.
  
  
  METHODS
  Hemangioma-derived endothelial cell line XTPS-1 was cultured with different concentration of bleomycin A5 (1000, 100, 10, 1, 0 mg/L), and then the survival rate was measured by methyl thiazolyl terazolium (MTT), the variation of cell morphology was observed using inverted phase contrast microscope and electron microscope, the variation of cell cycle and apoptosis rate were measured using flow cytometry.
  
  
  RESULTS
  After 24 hours culture the cell survival rate was (92.96 ± 3.66)% and (99.86 ± 0.12)% in lower saturation group (10 and 1 mg/L), but (34.08 ± 3.11)% and (43.28 ± 2.88)% in higher saturation group (1000 and 100 mg/L). The difference between them was more significant (P < 0.01). Lower saturation of bleomycin A5 (10 and 1 mg/L)could induce apoptosis but had almost no cytotoxic effect. Higher saturation of bleomycin A5 (1000 and 100 mg/L) not only induced apoptosis, but also had strong cytotoxic effect, which was concentration dependent.
  
  
  CONCLUSIONS
  bleomycin A5 could induce apoptosis, inhibit cell proliferation and has direct cytotoxic effect.
authors.affiliation: Department of Pediatric Surgery, The Second Affliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China. sienafiat@hotmail.com
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Xiao-e
authors.initials: XE
authors.lastname: Xiao
authors.forename: Zheng-tuan
authors.initials: ZT
authors.lastname: Guo
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001761:Bleomycin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000473:pathology*; D006391:Hemangioma / Q000473:pathology*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008858:Microscopy, Phase-Contrast
chemical_list: D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; C025703:bleomycetin