title: Lactobacillus pentosus var. plantarum C29 protects scopolamine-induced memory deficit in mice.
abstract: OBJECTIVE
  In the preliminary study, kimchi, a traditional food fermented with Chinese cabbage, protected scopolamine-induced mouse memory deficit in passive avoidance test. Therefore, we screened protective ingredients, particularly lactic acid bacteria, from Chinese cabbage kimchi against scopolamine-induced memory deficit in mice.
  
  
  RESULTS
  Lactic acid bacteria, isolated from Chinese cabbage kimchi, were identified by 16S rDNA sequence analysis, G+C content and cellular fatty acid composition and sugar fermentation test. Memory deficit was induced in mice by intraperitoneally injecting with scopolamine. Kimchi, particularly its supernatant, protected scopolamine-induced memory deficit in mice in passive avoidance test. Of kimchi ingredients, a lactic acid bacterium, strain C29, potently protected scopolamine-induced memory deficit in mice. C29 was a gram-positive, catalase-negative, anaerobic and non-motile rod. Its pylogenetic property was near to Lactobacillus pentosus (99%) and Lact. plantarum (99%). However, C29 fermented inulin and L-rhamnose and grew in pH 3 and at 45°C in contrast with Lact. pentosus and Lact. plantarum. Therefore, it named to be Lact pentosus var. plantarum C29. The strain C29 protected scopolamine-induced memory deficit in Y-maze and Morris water maze tests. Furthermore, C29 increased hippocampal BDNF and p-CREB expressions, which were reduced by scopolamine.
  
  
  CONCLUSIONS
  Lactobacillus pentosus var. plantarum C29 may protect memory deficit by inducing BDNF and p-CREB expressions.
  
  
  CONCLUSIONS
  Lactic acid bacteria, such as Lact pentosus var. plantarum C29, may prevent memory deficit and its contained fermented foods may be beneficial for dementia.
authors.affiliation: Department of Life and Nanopharmaceutical Sciences, Kyung Hee Univertisy, Seoul, Korea.
authors.forename: I-H
authors.initials: IH
authors.lastname: Jung
authors.forename: M-A
authors.initials: MA
authors.lastname: Jung
authors.forename: E-J
authors.initials: EJ
authors.lastname: Kim
authors.forename: M J
authors.initials: MJ
authors.lastname: Han
authors.forename: D-H
authors.initials: DH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001362:Avoidance Learning; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D001937:Brassica / Q000382:microbiology*; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D004275:DNA, Ribosomal / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D005516:Food Microbiology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced / Q000517:prevention & control*; D051379:Mice; D008813:Mice, Inbred ICR; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012601:Scopolamine / Q000009:adverse effects
chemical_list: D019208:Brain-Derived Neurotrophic Factor; C492004:Creb1 protein, mouse; D017362:Cyclic AMP Response Element-Binding Protein; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012601:Scopolamine