title: Investigating the role of FGF18 in the cultivation and osteogenic differentiation of mesenchymal stem cells.
abstract: Fibroblast growth factor18 (FGF18) belongs to the FGF family and is a pleiotropic protein that stimulates proliferation in several tissues. Bone marrow mesenchymal stem cells (BMSCs) participate in the normal replacement of damaged cells and in disease healing processes within bone and the haematopoietic system. In this study, we constructed FGF18 and investigated its effects on rat BMSCs (rBMSCs). The proliferative effects of FGF18 on rBMSCs were examined using an MTS assay. To validate the osteogenic differentiation effects of FGF18, ALP and mineralization activity were examined as well as osteogenic differentiation-related gene levels. FGF18 significantly enhanced rBMSCs proliferation (p<0.001) and induced the osteogenic differentiation by elevating ALP and mineralization activity of rBMSCs (p<0.001). Furthermore, these osteogenic differentiation effects of FGF18 were confirmed via increasing the mRNA levels of collagen type I (Col I), bone morphogenetic protein 4 (BMP4), and Runt-related transcription factor 2 (Runx2) at 3 and 7 days. These results suggest that FGF18 could be used to improve bone repair and regeneration.
authors.affiliation: Department of Biochemistry, Inha University School of Medicine, Incheon, Republic of Korea.
authors.forename: Eunyi
authors.initials: E
authors.lastname: Jeon
authors.forename: Ye-Rang
authors.initials: YR
authors.lastname: Yun
authors.forename: Wonmo
authors.initials: W
authors.lastname: Kang
authors.forename: Sujin
authors.initials: S
authors.lastname: Lee
authors.forename: Young-Hag
authors.initials: YH
authors.lastname: Koh
authors.forename: Young-Hyag
authors.initials: YH
authors.lastname: Koh
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
authors.forename: Chang Kook
authors.initials: CK
authors.lastname: Suh
authors.forename: Jun-Hyeog
authors.initials: JH
authors.lastname: Jang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D051381:Rats
chemical_list: C525811:Bmp4 protein, rat; D055415:Bone Morphogenetic Protein 4; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; C493717:Runx2 protein, rat; C113569:fibroblast growth factor 18; D005346:Fibroblast Growth Factors; D000469:Alkaline Phosphatase