title: Development of a novel primer combination to detect pathogenic Leptospira by loop-mediated isothermal amplification.
abstract: Loop-mediated isothermal amplification (LAMP) has been used for the detection of leptospiral 16S rDNA. Here we report the development of the LAMP method for leptospiral 16S rDNA detection which can be interpreted easily within 90 min by visualizing the fluorescence of calcein. The lower limit of detection (LLOD) is 10-100 copies.
authors.affiliation: National Institute of Animal Health, Department of Livestock Development, Bangkok 10900, Thailand. duangjais@dld.go.th
authors.forename: D
authors.initials: D
authors.lastname: Suwancharoen
authors.forename: C
authors.initials: C
authors.lastname: Kulchim
authors.forename: C
authors.initials: C
authors.lastname: Chirathaworn
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005452:Fluoresceins / Q000032:analysis; D005453:Fluorescence; D006801:Humans; D007919:Leptospira / Q000235:genetics / Q000302:isolation & purification*; D007922:Leptospirosis / Q000175:diagnosis* / Q000662:veterinary*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005452:Fluoresceins; D012336:RNA, Ribosomal, 16S; C007740:fluorexon