title: [Influence of Chlamydia trachomatis type III secretion system on regulation of cytokine response].
abstract: OBJECTIVE
  Develop in vitro model for studying production of cytokines by monocyte cells infected with Chlamydia trachomatis mediated by type III secretion system (TTSS).
  
  
  METHODS
  Strain C. trachomatis L2/434/Bu was used in the experiments, culture of human monocytes U-937 was infected by this strain. Level of inflammatory cytokines was measured on flow analyzer Bio-Plex 200 (Bio-Rad Laboratories). Low molecular compound LHC-342 which belongs to the class of heterocyclic compounds was used as TTSS inhibitor.
  
  
  RESULTS
  24 hours after the infection with C. trachomatis culture 8 analyzed cytokines are induced in U-937 cells (IL-1beta, IL-4, IL-6, IL-8, IL-10, GM-CSF, IFN-gamma, TNFalpha). The most pronounced increase was observed for IL-8, GM-CSF and IFN-gamma. Introduction of TTSS inhibitor into the culture of infected cells suppressed chlamydia growth, but addition of FeSO4 restored the growth of chlamydiae. And activity associated with translocation of effector TTSS protein IncA to inclusion membrane was suppressed. Under the conditions of the obtained model of TTSS inhibition during intracellular development of C. trachomatis a significant decrease of 2 pro-inflammatory cytokines--IL-6 and IL-1beta--was observed.
  
  
  CONCLUSIONS
  Cytokine response plays a key role in the protective immune response in chlamydia infection but at the same time induces immunopathologic conditions. The data obtained give reasons to assume role of C. trachomatis TTSS in the induction of this component of immune response that requires further detailed studies.
authors.forename: E D
authors.initials: ED
authors.lastname: Fedina
authors.forename: N I
authors.initials: NI
authors.lastname: Kolkova
authors.forename: E A
authors.initials: EA
authors.lastname: Koroleva
authors.forename: L A
authors.initials: LA
authors.lastname: Shabalina
authors.forename: V I
authors.initials: VI
authors.lastname: Grabko
authors.forename: N A
authors.initials: NA
authors.lastname: Zigangirova
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D058947:Bacterial Secretion Systems / Q000187:drug effects* / Q000276:immunology; D002460:Cell Line; D002692:Chlamydia trachomatis / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology*; D005296:Ferrous Compounds / Q000494:pharmacology; D005434:Flow Cytometry; D006571:Heterocyclic Compounds / Q000494:pharmacology; D006801:Humans; D008565:Membrane Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D016207:Cytokines; D005296:Ferrous Compounds; D006571:Heterocyclic Compounds; C460868:IncA protein, Chlamydia trachomatis; D008565:Membrane Proteins