title: In vitro evaluation of an RGD-functionalized chitosan derivative for enhanced cell adhesion.
abstract: Tissue repair is a spontaneous process that is initiated on wounding. However, if this complex mechanism is impaired or not sufficient the use of biomaterials might increase the chance of successful healing. In this view, an RGD-functionalized polymer was developed to promote dermal healing. A water-soluble chitosan derivative, carboxymethyl-trimethylchitosan (CM-TM-chitosan) was synthesized and GRGDS-moieties were grafted to the backbone at a concentration of 59 nmol/mg polymer to increase cell-biomaterial interaction. Tested in vitro with cultured human dermal fibroblasts, the developed polymer showed good biocompatibility and the initial adhesion was increased by 3-5 times due to the GRGDS-moieties. Moreover, cell spreading was specific to the interaction with GRGDS, giving a 12-fold increase of cells showing a fully spread morphology within 30 min. Overall, CM-TM-chitosan conjugated with GRGDS-peptides may prove useful as a biomaterial in wound healing.
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest Ansermet 30, 1211 Geneva, Switzerland.
authors.forename: Annasara
authors.initials: A
authors.lastname: Hansson
authors.forename: Nour
authors.initials: N
authors.lastname: Hashom
authors.forename: Françoise
authors.initials: F
authors.lastname: Falson
authors.forename: Patricia
authors.initials: P
authors.lastname: Rousselle
authors.forename: Olivier
authors.initials: O
authors.lastname: Jordan
authors.forename: Gerrit
authors.initials: G
authors.lastname: Borchard
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology*; D002470:Cell Survival; D002478:Cells, Cultured; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D020405:Dermis / Q000166:cytology / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009842:Oligopeptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D009842:Oligopeptides; D011108:Polymers; C514968:carboxymethyl-chitosan; D048271:Chitosan; C049554:glycyl-arginyl-glycyl-aspartyl-serine