title: Kinetic transcriptomic approach revealed metabolic pathways and genotoxic-related changes implied in the Arabidopsis response to ionising radiations.
abstract: Plants exposed to ionising radiation (IR) have to face direct and indirect (oxidative stress) deleterious effects whose intensity depends on the dose applied and led to differential genome regulation. Transcriptomic analyses were conducted with CATMA microarray technology on Arabidopsis thaliana plantlets, 2 and 26h after exposure to the IR doses 10Gy and 40Gy. 10Gy treatment seemed to enhance antioxidative compound biosynthetic pathways whereas the 40Gy dose up-regulated ROS-scavenging enzyme genes. Transcriptomic data also highlighted a differential regulation of chloroplast constituent genes depending on the IR dose, 10Gy stimulating and 40Gy down-regulating. This probable 40Gy decrease of photosynthesis could help for the limitation of ROS production and may be coupled with programmed cell death (PCD)/senescence phenomena. Comparisons with previous transcriptomic studies on plants exposed to a 100Gy dose revealed 60 dose-dependent up-regulated genes, including notably cell cycle checkpoints to allow DNA repairing phenomena. Furthermore, the alteration of some cellular structure related gene expression corroborated a probable mitotic arrest after 40Gy. Finally, numerous heat-shock protein and chaperonin genes, known to protect proteins against stress-dependent dysfunction, were up-regulated after IR exposure.
authors.affiliation: Mechanisms and Origin of Biodiversity Team, UMR 6553-Ecobio, University of Rennes1, 263 Av. du Général Leclerc, Campus de Beaulieu-Bât.14A, 35042 Rennes, France.
authors.forename: Morgane
authors.initials: M
authors.lastname: Gicquel
authors.forename: Ludivine
authors.initials: L
authors.lastname: Taconnat
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Renou
authors.forename: Marie-Andrée
authors.initials: MA
authors.lastname: Esnault
authors.forename: Francisco
authors.initials: F
authors.lastname: Cabello-Hurtado
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics / Q000528:radiation effects; D016922:Cellular Senescence / Q000235:genetics / Q000528:radiation effects; D018833:Chaperonins / Q000235:genetics / Q000378:metabolism; D002736:Chloroplasts / Q000235:genetics; D004260:DNA Repair / Q000235:genetics / Q000528:radiation effects; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000528:radiation effects*; D017343:Genes, Plant / Q000528:radiation effects*; D018745:Genome, Plant / Q000528:radiation effects; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000235:genetics / Q000528:radiation effects; D008938:Mitosis / Q000235:genetics / Q000528:radiation effects; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D018384:Oxidative Stress / Q000235:genetics / Q000528:radiation effects; D010788:Photosynthesis / Q000528:radiation effects; D011839:Radiation, Ionizing; D017382:Reactive Oxygen Species / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics* / Q000528:radiation effects
chemical_list: D000975:Antioxidants; D029681:Arabidopsis Proteins; D004798:Enzymes; D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species; D018833:Chaperonins