title: Enhancement of surface graft density of MPEG on alginate/chitosan hydrogel microcapsules for protein repellency.
abstract: Alginate/chitosan/alginate (ACA) hydrogel microcapsules were modified with methoxy poly(ethylene glycol) (MPEG) to improve protein repellency and biocompatibility. Increased MPEG surface graft density (n(S)) on hydrogel microcapsules was achieved by controlling the grafting parameters including the buffer layer substrate, membrane thickness, and grafting method. X-ray photoelectron spectroscopy (XPS) model was employed to quantitatively analyze n(S) on this three-dimensional (3D) hydrogel network structure. Our results indicated that neutralizing with alginate, increasing membrane thickness, and in situ covalent grafting could increase n(S) effectively. ACAC(PEG) was more promising than ACC(PEG) in protein repellency because alginate supplied more -COO(-) negative binding sites and prevented MPEG from diffusing. The n(S) increased with membrane thickness, showing better protein repellency. Moreover, the in situ covalent grafting provided an effective way to enhance n(S), and 1.00 ± 0.03 chains/nm(2) was achieved, exhibiting almost complete immunity to protein adsorption. This antifouling hydrogel biomaterial is expected to be useful in transplantation in vivo.
authors.affiliation: Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Jiani
authors.initials: J
authors.lastname: Zheng
authors.forename: Hongguo
authors.initials: H
authors.lastname: Xie
authors.forename: Weiting
authors.initials: W
authors.lastname: Yu
authors.forename: Mingqian
authors.initials: M
authors.lastname: Tan
authors.forename: Faquan
authors.initials: F
authors.lastname: Gong
authors.forename: Xiudong
authors.initials: X
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Guojun
authors.initials: G
authors.lastname: Lv
authors.forename: Wanfa
authors.initials: W
authors.lastname: Liu
authors.forename: Guoshuang
authors.initials: G
authors.lastname: Zheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Weiyang
authors.initials: W
authors.lastname: Xie
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
mesh_terms: D000327:Adsorption; D000464:Alginates / Q000737:chemistry*; D002214:Capsules; D048271:Chitosan / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D007074:Immunoglobulin G; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D020723:Glucuronic Acid; D005340:Fibrinogen; C028210:monomethoxypolyethylene glycol; D048271:Chitosan