title: Quantitative proteomic analysis of differentially expressed proteins in the toxicity-lost mutant of Alexandrium catenella (Dinophyceae) in the exponential phase.
abstract: Alexandrium catenella is a widely spread dinoflagellate species which can produce potent neurotoxins and result in paralytic shellfish poisoning. To date, the molecular mechanisms regulating toxin biosynthesis remain unclear. This study compared protein profiles of a toxicity-lost mutant of A. catenella, ACHK-NT and its wild-type, ACHK-T in the exponential phase, using two dimensional differential gel electrophoresis and MALDI-TOF-TOF mass spectrometry. Morphological analysis showed that both subcultures were morphologically identical with the distinctive taxonomic characteristics of A. catenella. Sequence analyses of ITS1, 5.8S, ITS2 and 18S demonstrated that the mutant and wild subcultures were genetically identical for these markers. 90 differentially expressed protein spots were identified from ACHK-NT, of which 34 were down-regulated and 56 were up-regulated. Using a multilayered strategy for de novo protein sequence analysis, 67 proteins assigned to different functional categories were identified. Among them, 25 involved in bioluminescence, secondary metabolite biosynthesis, protein modification and toxin biosynthesis were down-regulated, while 42 participating in carbon fixation, stress response, transporter and protein folding were up-regulated. This study indicated that the strengthening of certain biological processes coupled with the depression of essential reactions upstream or downstream of the toxin biosynthetic pathway might have blocked toxin production and resulted in the loss of toxicity in the mutant A. catenella.
authors.affiliation: State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen 361005, China. dzwang@xmu.edu.cn
authors.forename: Da-Zhi
authors.initials: DZ
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yu-Yu
authors.initials: YY
authors.lastname: Wang
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: He
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Hua-Sheng
authors.initials: HS
authors.lastname: Hong
mesh_terms: D001483:Base Sequence; D004141:Dinoflagellida / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D008387:Marine Toxins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009154:Mutation; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D057096:Shellfish Poisoning; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008387:Marine Toxins; D011506:Proteins