title: Microsatellite markers developed for Utricularia reniformis (Lentibulariaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed to study the genetic diversity and population structure of the carnivorous bladderwort Utricularia reniformis, which is endemic to the Atlantic Forest of southern and southeastern Brazil. Cross-species amplification was tested in U. gibba, U. neottioides, U. subulata, and Pinguicula benedicta.
  
  
  RESULTS
  The U. reniformis genome was sequenced in a 454 GS FLX sequencer, and eight primer sets were developed based on the microsatellites identified from the reads. All loci are polymorphic, showing 1.6 to 4.8 alleles per population. Preliminary results show that primer sets are suitable for population-level studies. Cross-species amplification was successful in three other Utricularia species and one Pinguicula species.
  
  
  CONCLUSIONS
  Markers developed in this study provide tools for analyses of intra- and interpopulation genetic diversity in Utricularia and Pinguicula.
authors.affiliation: Universidade de Mogi das Cruzes, Núcleo Integrado de Biotecnologia, Av. Cândido Xavier de Almeida e Souza 200, Mogi das Cruzes, São Paulo 08780-911, Brazil. deboraclivati@yahoo.com.br
authors.forename: Débora
authors.initials: D
authors.lastname: Clivati
authors.forename: Matthew Aaron
authors.initials: MA
authors.lastname: Gitzendanner
authors.forename: Alexandre Wagner Silva
authors.initials: AW
authors.lastname: Hilsdorf
authors.forename: Welington Luiz
authors.initials: WL
authors.lastname: Araújo
authors.forename: Vítor Fernandes
authors.initials: VF
authors.lastname: Oliveira de Miranda
mesh_terms: D000483:Alleles; D001938:Brazil; D017931:DNA Primers / Q000378:metabolism; D005787:Gene Frequency / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D019686:Lamiaceae / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D013045:Species Specificity
chemical_list: D017931:DNA Primers