title: [An improved method for primary culture of mouse pulmonary microvascular endothelial cells].
abstract: OBJECTIVE
  To establish an improved method for culturing primary mouse pulmonary microvascular endothelial cells (PMVECs).
  
  
  METHODS
  An improved tissue block adherent culture method was used to isolate and culture the PMVECs from C57 mice. The cultured cells were identified by factor VIII-related antigen and CD31 antigen, and the growth of cells cultured using the improved method and the conventional method was compared.
  
  
  RESULTS
  The cultured primary pulmonary microvascular endothelial cells showed a short fusiform or round morphology, and the cell monolayer displayed a cobble stone-like appearance. The cultured cells were positive for VIII-related antigen and CD31 antigen. The cell growth was accelerated in the cell cultures with the improved method compared with that in conventional cell cultures.
  
  
  CONCLUSIONS
  The improved culture method allows more efficient acquisition of primary mouse PMVECs of a greater purity.
authors.affiliation: Department of Respiratory Diseases, Southern Medical University, Guangzhou, China. 956324887@qq.com
authors.forename: Guodong
authors.initials: G
authors.lastname: Hu
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Chen
authors.forename: Aihua
authors.initials: A
authors.lastname: Liu
authors.forename: Guohong
authors.initials: G
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Kang
authors.forename: Shaoxi
authors.initials: S
authors.lastname: Cai
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology*; D008168:Lung / Q000098:blood supply; D051379:Mice; D008810:Mice, Inbred C57BL; D061251:Primary Cell Culture / Q000379:methods*