title: Sample preparation for the analysis of membrane proteomes by mass spectrometry.
abstract: The low abundance and highly hydrophobic nature of most membrane proteins make their analysis more difficult than that for common soluble proteins. Successful membrane protein identification is largely dependent on the sample preparation including the enrichment and dissolution of the membrane proteins. A series of conventional and newly developed methods has been applied to the enrichment of low-abundance membrane proteins at membrane and/or protein levels and to the dissolution of hydrophobic membrane proteins. However, all the existing methods have inherent advantages and limitations. Up to now, there has been no unique method that can universally be employed to solve all the problems and more efforts are needed in improving sample preparation for the analysis of membrane proteomes.
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Xianchun
authors.initials: X
authors.lastname: Wang
authors.forename: Songping
authors.initials: S
authors.lastname: Liang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D008567:Membranes, Artificial; D010084:Oxidation-Reduction; D011145:Polyvinyls / Q000737:chemistry; D020543:Proteome / Q000032:analysis; D040901:Proteomics; D005465:Fluorocarbon Polymers
chemical_list: D008565:Membrane Proteins; D008567:Membranes, Artificial; D011145:Polyvinyls; D020543:Proteome; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers