title: Purification, crystallization and preliminary X-ray analysis of SGR6054, a Streptomyces homologue of the mycobacterial integration host factor mIHF.
abstract: The mycobacterial integration host factor (mIHF) is a small nonspecific DNA-binding protein that is essential for the growth of Mycobacterium smegmatis. mIHF homologues are widely distributed among Actinobacteria, and a Streptomyces homologue of mIHF is involved in control of sporulation and antibiotic production in S. coelicolor A3(2). Despite their important biological functions, a structure of mIHF or its homologues has not been elucidated to date. Here, the S. griseus mIHF homologue (SGR6054) was expressed and purified from Escherichia coli and crystallized in the presence of a 16-mer duplex DNA by the sitting-drop vapour-diffusion method. The plate-shaped crystal belonged to space group C2, with unit-cell parameters a = 88.53, b = 69.35, c = 77.71 Å, β = 96.63°, and diffracted X-rays to 2.22 Å resolution.
authors.affiliation: Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Nomoto
authors.forename: Takeaki
authors.initials: T
authors.lastname: Tezuka
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Miyazono
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanokura
authors.forename: Sueharu
authors.initials: S
authors.lastname: Horinouchi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ohnishi
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D034982:Integration Host Factors / Q000737:chemistry* / Q000302:isolation & purification; D048372:Streptomyces coelicolor / Q000737:chemistry*
chemical_list: D034982:Integration Host Factors