title: Characterization of diverse antimicrobial peptides in skin secretions of Chungan torrent frog Amolops chunganensis.
abstract: We have cloned, synthesized, and characterized 11 novel antimicrobial peptides from a skin derived cDNA library of the Chungan torrent frog, Amolops chunganensis. Seven of the 11 antimicrobial peptides were present in authentic A. chunganensis skin secretions. Sequence analysis indicated that the 11 peptides belonged to the temporin, esculentin-2, palustrin-2, brevinin-1, and brevinin-2 families. The peptides displayed potent antimicrobial activities against several strains of microorganisms. One peptide, brevinin-1CG5, demonstrated antimicrobial activity against all tested Gram-positive and Gram-negative bacteria and fungi, and showed high antimicrobial potency (MIC=0.6 μM) against Gram-positive bacterium Rhodococcus rhodochrous. Some peptides also demonstrated weak hemolytic activity against human erythrocytes in vitro. Phylogenetic analysis based on the amino acid sequences of brevinin-1, brevinin-2, and esculentin-2 peptides from family Ranidae confirmed that the current taxonomic status of A. chunganensis is correct.
authors.affiliation: Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050024, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yang
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Xia
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Yu
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Hu
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Meng
authors.forename: Shujie
authors.initials: S
authors.lastname: Yang
authors.forename: Jingze
authors.initials: J
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics; D012634:Bodily Secretions / Q000737:chemistry; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D053878:Hemolytic Agents / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010802:Phylogeny; D011506:Proteins / Q000235:genetics; D011898:Ranidae; D012867:Skin / Q000737:chemistry*
chemical_list: D029845:Amphibian Proteins; D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D053878:Hemolytic Agents; D011506:Proteins; C092874:esculentin protein, Rana esculenta; C104501:temporin; C078413:brevinin-1 protein, Rana; C078414:brevinin-2, Rana