title: Chloroplast ultrastructure regeneration with protection of photosystem II is responsible for the functional 'stay-green' trait in wheat.
abstract: CN17 is a functional stay-green wheat variety that exhibits delayed leaf senescence and enhanced photosynthetic competence. To better understand these valuable traits, levels of chlorophyll a and b, soluble proteins, unsaturated fatty acids, and other components of CN17 were assayed. In addition, chloroplast ultrastructure, chloroplast number, and differences in gene expression between CN17 and a control variety, MY11, were examined. By 21 d post-anthesis (DPA), CN17 leaves exhibited a significantly higher maximal photochemical efficiency for photosystem II (PSII) (F(v) /F(m) ) and a significantly higher efficiency of excitation capture by open PSII reaction centres (F(v) '/F(m) '). In addition, chlorophyll degradation in CN17 was delayed by approximately 14 d, and was not blocked as observed in cosmetic stay-green phenotypes. The soluble protein content (Ps) of CN17 was higher than MY11 at all timepoints assayed, and the ratio of unsaturated to saturated fatty acids was significantly higher. CN17 also exhibited isolated granal lamellae associated with vesicles and diminished peroxidation, and between 35 and 42 DPA, a sharp decrease in chloroplast number was detected. Taken together, these results strongly support the hypothesis that chloroplast ultrastructure regeneration is responsible for the functional stay-green trait of CN17, and gene expression data provide insight into the mechanistic details.
authors.affiliation: State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Ya'an, Sichuan 625014, China. lpglab@sicau.edu.cn
authors.forename: P G
authors.initials: PG
authors.lastname: Luo
authors.forename: K J
authors.initials: KJ
authors.lastname: Deng
authors.forename: X Y
authors.initials: XY
authors.lastname: Hu
authors.forename: L Q
authors.initials: LQ
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: J B
authors.initials: JB
authors.lastname: Chen
authors.forename: H Y
authors.initials: HY
authors.lastname: Zhang
authors.forename: Z X
authors.initials: ZX
authors.lastname: Tang
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: Q X
authors.initials: QX
authors.lastname: Sun
authors.forename: F Q
authors.initials: FQ
authors.lastname: Tan
authors.forename: Z L
authors.initials: ZL
authors.lastname: Ren
mesh_terms: D002734:Chlorophyll / Q000378:metabolism*; D000077194:Chlorophyll A; D002736:Chloroplasts / Q000502:physiology / Q000648:ultrastructure*; D020224:Expressed Sequence Tags; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D015723:Gene Library; D010788:Photosynthesis; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D018515:Plant Leaves / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D014908:Triticum / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D005231:Fatty Acids, Unsaturated; D045332:Photosystem II Protein Complex; D017382:Reactive Oxygen Species; D002734:Chlorophyll; C037184:chlorophyll b; D000077194:Chlorophyll A