title: Structural and spectroscopic features of lutein/butanoyl-β-cyclodextrin nanoassemblies.
abstract: Lutein, the primary carotenoid present in the central area of the retina of eye appears to be associated with the protection against age-related macular degeneration (the leading cause of blindness in older adults). Its lipophilicity and consequently its scarce water solubility (1.3×10(-9)M) represent a drawback for bioavailability. To circumvent these unfavorable characteristics, in this work lutein (Lut) have been encapsulated in amphiphilic cyclodextrin (ACyD) by following the well-established strategy of entrapping a lipophilic drug in CyD carriers. Primary face butyrate modified β-cyclodextrins (C(4:7)) form in water nanoaggregates with a average size of 250nm and a ζ-potential of about -6mV. They are able to entrap lutein at 1:6 Lut/ACyD molar ratio by yielding nanoassemblies of vesicular aspect (320nm and -8mV) such as observed by static, dynamic and electrophoretic light-scattering. UV-vis measurements revealed that electronic properties of lutein were maintained when interact with ACyD nanoaggregates. The monitoring of the entapped carotenoid leaking from ACyD nanostructures was investigated suggesting the potential of Lut/ACyD nanoassemblies in drug delivery.
authors.affiliation: Dipartimento Farmaco-Chimico, Università di Messina, V.le Annunziata, 98168 Messina, Italy. rstancanelli@unime.it
authors.forename: Rosanna
authors.initials: R
authors.lastname: Stancanelli
authors.forename: Lars Damgaard
authors.initials: LD
authors.lastname: Løjkner
authors.forename: Kim Lambertsen
authors.initials: KL
authors.lastname: Larsen
authors.forename: Marta
authors.initials: M
authors.lastname: Guardo
authors.forename: Carmela
authors.initials: C
authors.lastname: Cannavà
authors.forename: Silvana
authors.initials: S
authors.lastname: Tommasini
authors.forename: Cinzia Anna
authors.initials: CA
authors.lastname: Ventura
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Calabrò
authors.forename: Norberto
authors.initials: N
authors.lastname: Micali
authors.forename: Valentina
authors.initials: V
authors.lastname: Villari
authors.forename: Antonino
authors.initials: A
authors.lastname: Mazzaglia
mesh_terms: D001682:Biological Availability; D002338:Carotenoids / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D014975:Lutein / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D047392:beta-Cyclodextrins; D014867:Water; D002338:Carotenoids; D014975:Lutein