title: DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis.
abstract: Pulsed-field gel electrophoresis (PFGE) is a technique for resolving large (up to 10 Mb) DNA molecules. Using multiple pairs of electrodes DNA is subject to an alternating electric field through a solid agarose matrix. As the current changes direction the reorientation time of DNA is proportional to molecular weight; thus fragments are separated in the gel based on their size. Here we describe the use of PFGE to analyze DNA double-strand break formation and repair in human chromosomal DNA.
authors.affiliation: Department of Oncology, The Institute for Cancer Studies,University of Sheffield, Sheffield, UK. h.bryant@sheffield.ac.uk
authors.forename: Helen E
authors.initials: HE
authors.lastname: Bryant
mesh_terms: D002877:Chromosomes, Human / Q000235:genetics; D053903:DNA Breaks, Double-Stranded / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans