title: Phosphoproteome profiling of the sexually transmitted pathogen Trichomonas vaginalis.
abstract: OBJECTIVE
  Trichomoniasis caused by Trichomonas vaginalis is the most common non-viral sexually transmitted infection. Morphological transformation from the trophozoite stage to the amoeboid or pseudocyst stage is crucial for T. vaginalis infection and survival. Protein phosphorylation is a key post-translational modification involved in the regulation of several biological processes in various prokaryotes and eukaryotes. More than 880 protein kinases have been identified in the T. vaginalis genome. However, little is known about the phosphorylation of specific proteins and the distribution of phosphorylated proteins in different stages of the morphological transformation of T. vaginalis.
  
  
  METHODS
  To obtain a more comprehensive understanding of the T. vaginalis phosphoproteome, we analyzed phosphorylated proteins in the three morphological stages using titanium dioxide combined with LC-MS/MS.
  
  
  RESULTS
  A total of 93 phosphopeptides originating from 82 unique proteins were identified. Among these proteins, 21 were detected in all stages, 29 were identified in two different stages, and 32 were stage specific.
  
  
  CONCLUSIONS
  Identification of stage-specific phosphorylated proteins indicates that phosphorylation of these proteins may play a key role in the morphological transformation of T. vaginalis.
authors.affiliation: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Yeh
authors.forename: Kuo-Yang
authors.initials: KY
authors.lastname: Huang
authors.forename: Ruei-Chi
authors.initials: RC
authors.lastname: Richie Gan
authors.forename: Hsien-Da
authors.initials: HD
authors.lastname: Huang
authors.forename: Tzu-Chien V
authors.initials: TC
authors.lastname: Wang
authors.forename: Petrus
authors.initials: P
authors.lastname: Tang
mesh_terms: D002853:Chromatography, Liquid; D020869:Gene Expression Profiling; D010750:Phosphoproteins / Q000032:analysis*; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis*; D015800:Protozoan Proteins / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014246:Trichomonas vaginalis / Q000737:chemistry* / Q000254:growth & development
chemical_list: D010750:Phosphoproteins; D020543:Proteome; D015800:Protozoan Proteins