title: [Archaeal diversity in sediment of Dunbasitawu salt lake, Xinjiang, China, estimated by 16S rRNA gene sequence analyses].
abstract: OBJECTIVE
  We surveyed the composition and diversity of uncultured archaea in Xinjiang Dunbasitawu salt Lake sediment.
  
  
  METHODS
  Environmental total DNA was directly extracted from the sediment. We constructed clone library of 16S rRNA gene amplified with archaea-specific primers. A total of 59 positive clones were randomly selected from the library and identified by restriction length polymorphism (RFLP) with enzyme Hae III. Clones with the unique RFLP pattern were sequenced, and then by phylogenetic analysis.
  
  
  RESULTS
  The clone coverage C value was 89%, and Shannon-Wiener index was 2.69. In total, 21 Operational Taxonomic Units (OTUs) were obtained and affiliated with Euryarchaeota (92%) and Crenarchaeota (8%). The most of clones were affiliated to Halobacterium (24%), Haloarcula (18%), Natronorubrum (14%), and Halorubrum (8%), which belonged to family Halobacteriaceae (88%) with high similarity to that from thalassohaline environment. In addition, 11% of clones had less than 97% similarity with archaea sequences deposited in GenBank database.
  
  
  CONCLUSIONS
  Compared with other similar Hypersaline environments, archaea diversity in Dunbasitawu salt lake was a little lower. The proportion of archara was different, but the composition is consistent. It was implied that some potential new species or lineages maybe exist in Dunbasitawu salt lake.
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumqi 830046, China. hello466725@126.com
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.forename: Hongmei
authors.initials: H
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Xianyan
authors.initials: X
authors.lastname: Liao
authors.forename: Lijuan
authors.initials: L
authors.lastname: Deng
authors.forename: Shan
authors.initials: S
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Lou
authors.forename: Erkin
authors.initials: E
authors.lastname: Rahman
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics; D044822:Biodiversity; D002681:China; D019615:Crenarchaeota / Q000145:classification / Q000235:genetics; D019605:Euryarchaeota / Q000145:classification / Q000235:genetics; D015723:Gene Library; D019015:Geologic Sediments / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S