title: Integrin-linked kinase functions as a tumor promoter in bladder transitional cell carcinoma.
abstract: The aim of this study was to elucidate the role of the integrin-linked kinase (ILK) gene in development of human bladder transitional cell carcinoma (BTCC). Expression of ILK protein and ILK mRNA in 56 cases of human BTCC tissue and in 30 cases of adjacent normal bladder tissue was detected by immunohistochemistry S-P and reverse transcription polymerase chain reaction (RT-PCR), respectively. Four specific miRNA RNAi vectors targeting human ILK were synthesized and transfected into BIU-87 cells by liposome to obtain stable expression cell strains. The influence of ILK on proliferation of BTCC was detected by MTT, FCM on athymic mouse tumorigenesis. The positive rate of ILK protein in BTCC tissue (53.6%) was much higher than adjacent normal bladder tissue (10.0%) (p<0.05). Similarly, expression of ILK mRNA in BTCC tissue (0.540 ± 0.083) was significantly higher than in adjacent normal bladder tissue (0.492 ± 0.070) (p<0.05). MTT showed that the proliferation ability of miRNA-ILK transfected group was clearly decreased (p<0.05), the cell cycle being arrested in G0/G1-S, an tumorigenesis in vivo was also significantly reduced (p<0.05). ILK gene transcription and protein expression may be involved in the development of BTCC, so that ILK might be the new marker for early diagnosis and the new target for gene treatment.
authors.affiliation: Department of Urology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China. dlwangws@sina.com
authors.forename: De-Lin
authors.initials: DL
authors.lastname: Wang
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Lan
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Biao
authors.initials: B
authors.lastname: Huang
authors.forename: Zeng
authors.initials: Z
authors.lastname: Li
authors.forename: Xiu-Min
authors.initials: XM
authors.lastname: Zhao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.forename: Xia
authors.initials: X
authors.lastname: Sheng
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Li
authors.forename: Wei-Xue
authors.initials: WX
authors.lastname: Tang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002273:Carcinogens; D002295:Carcinoma, Transitional Cell / Q000201:enzymology* / Q000235:genetics*; D059447:Cell Cycle Checkpoints / Q000235:genetics; D002455:Cell Division; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering; D014158:Transcription, Genetic / Q000235:genetics; D014183:Transplantation, Heterologous; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000235:genetics*; D000099200:Scaffold Protein ILK
chemical_list: D002273:Carcinogens; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000099200:Scaffold Protein ILK