title: Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice.
abstract: Pneumoviruses such as pneumonia virus of mice (PVM), bovine respiratory syncytial virus (bRSV) or human (h)RSV are closely related pneumoviruses that cause severe respiratory disease in their respective hosts. It is well-known that T-cell responses are essential in pneumovirus clearance, but pneumovirus-specific T-cell responses also are important mediators of severe immunopathology. In this study we determined whether memory- or pre-existing, transferred virus-specific CD8(+) T-cells provide protection against PVM-induced disease. We show that during infection with a sublethal dose of PVM, both natural killer (NK) cells and CD8(+) T-cells expand relatively late. Induction of CD8(+) T-cell memory against a single CD8(+) T-cell epitope, by dendritic cell (DC)-peptide immunization, leads to partial protection against PVM challenge and prevents Th2 differentiation of PVM-induced CD4 T-cells. In addition, adoptively transferred PVM-specific CD8(+) T-cells, covering the entire PVM-specific CD8(+) T-cell repertoire, provide partial protection from PVM-induced disease. From these data we infer that antigen-specific memory CD8(+) T-cells offer significant protection to PVM-induced disease. Thus, CD8(+) T-cells, despite being a major cause of PVM-associated pathology during primary infection, may offer promising targets of a protective pneumovirus vaccine.
authors.affiliation: Division of Immunology, University of Utrecht, Yalelaan 1, 3584 CL Utrecht, The Netherlands.
authors.forename: Mary J G
authors.initials: MJ
authors.lastname: van Helden
authors.forename: Peter J S
authors.initials: PJ
authors.lastname: van Kooten
authors.forename: Cornelis P J
authors.initials: CP
authors.lastname: Bekker
authors.forename: Andrea
authors.initials: A
authors.lastname: Gröne
authors.forename: David J
authors.initials: DJ
authors.lastname: Topham
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Easton
authors.forename: Claire J P
authors.initials: CJ
authors.lastname: Boog
authors.forename: Dirk H
authors.initials: DH
authors.lastname: Busch
authors.forename: Dietmar M W
authors.initials: DM
authors.lastname: Zaiss
authors.forename: Alice J A M
authors.initials: AJ
authors.lastname: Sijts
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D005260:Female; D007156:Immunologic Memory; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D029122:Murine pneumonia virus / Q000276:immunology*; D018186:Pneumovirus Infections / Q000276:immunology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology
chemical_list: D015847:Interleukin-4; D007371:Interferon-gamma