title: The spatiotemporal localization of JAM-C following sciatic nerve crush in adult rats.
abstract: JAM-C is a junctional adhesion molecule, enriched at tight junctions on endothelial and epithelial cells, and also localized to Schwann cells at junctions between adjoining myelin end loops. The role of JAM-C following peripheral nerve injury (PNI) is currently unknown. We examined the localization of JAM-C after sciatic nerve crush injury in adult rats. JAM-C immunoreactivity was present in paranodes and incisures in sham surgery control nerve, but distal to the crush injury significantly decreased at three and 14 days. JAM-C was re-expressed at 28 days and, by 56 days, was significantly increased in the distal nerve compared to controls. In a 7-mm length of sciatic nerve sampled distal to the crush site, the densities of JAM-C immunoreactive paranodes increased in the distal direction. Conversely, the densities of JAM-C immunoreactive incisures were highest immediately distal to the crush site and decreased in the more distal direction. Further analysis revealed a strong correlation between JAM-C localization and remyelination. Fifty-six days after crush injury, greater densities of JAM-C paranodes were seen compared to the nodal marker jacalin, suggesting that paranodal JAM-C precedes node formation. Our data are the first to demonstrate a potential role of JAM-C in remyelination after PNI.
authors.forename: Parizad
authors.initials: P
authors.lastname: Avari
authors.forename: Wenlong
authors.initials: W
authors.lastname: Huang
authors.forename: Sharon
authors.initials: S
authors.lastname: Averill
authors.forename: Bartomeu
authors.initials: B
authors.lastname: Colom
authors.forename: Beat A
authors.initials: BA
authors.lastname: Imhof
authors.forename: Sussan
authors.initials: S
authors.lastname: Nourshargh
authors.forename: John V
authors.initials: JV
authors.lastname: Priestley