title: [Effect of down-regulation of histone deacetylase 2 protein expression on cell proliferation and cell cycle in cervical carcinoma].
abstract: OBJECTIVE
  To study the effect of down-regulation of histone deacetylase 2 (HDAC2) expression on cell proliferation and cell cycle in cervical carcinoma cell lines HeLa.
  
  
  METHODS
  HDAC2 siRNA and control siRNA were transfected to HeLa cells. CCK-8 and flow cytometry were used to analyze the changes of cell proliferation and cell cycle, respectively. Western blot was employed to detect the changes of cell proliferation and cell cycle-related proteins.
  
  
  RESULTS
  HDAC2 siRNA significantly down-regulated the expression of HDAC2 protein in HeLa cells, resulting in marked inhibition of cell proliferation. In addition, the percentage of cells in G(0)/G(1) phase in HDAC2 siRNA group (63.3% ± 2.0%) was significantly higher than that in untreated group (29.3% ± 1.7%) or control siRNA group (29.4% ± 1.7%), F = 354.181, P = 0.000. Furthermore, Western blot demonstrated that down-regulation of HDAC2 expression decreased the expression of cyclin D1, cyclin E and CDK2 proteins but increased the expression of p21 protein.
  
  
  CONCLUSIONS
  Down-regulation of HDAC2 expression mediates proliferation inhibition and cell cycle arrest. It is associated with decrease in cyclin D1, cyclin E and CDK2 protein expression and increase in p21 protein expression.
authors.affiliation: Department of Obstetrics and Gynecology, the First Affiliated Hospital of Xinxiang Medical University, Weihui, China.
authors.forename: Fang-fang
authors.initials: FF
authors.lastname: Hua
authors.forename: Yong-hua
authors.initials: YH
authors.lastname: Xia
authors.forename: Da-peng
authors.initials: DP
authors.lastname: Wu
authors.forename: Rui-xiang
authors.initials: RX
authors.lastname: Chen
authors.forename: Yu-hong
authors.initials: YH
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Pan
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Wu-feng
authors.initials: WF
authors.lastname: Liang
mesh_terms: D002453:Cell Cycle; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D051357:Cyclin-Dependent Kinase 2 / Q000378:metabolism; D015536:Down-Regulation; D006367:HeLa Cells; D056464:Histone Deacetylase 2 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015513:Oncogene Proteins / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: C530104:CCND1 protein, human; C475820:CCNE1 protein, human; D019927:Cyclin E; D015513:Oncogene Proteins; D034741:RNA, Small Interfering; D019938:Cyclin D1; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; C533423:HDAC2 protein, human; D056464:Histone Deacetylase 2; D016283:Proto-Oncogene Proteins p21(ras)