title: The role of endocytic recycling in autoimmunity.
abstract: Abnormalities in T cell signal transduction underlie pathology in systemic lupus erythematosus. Lupus T cells are more sensitive to stimulation, yet have reduced expression of T cell antigen receptor (TCR) at the surface. The amount of TCR expressed at the surface of a T cell directly determines the ability of a T cell to become activated. The endocytic recycling machinery regulates transport of T cell receptors to the plasma membrane, internalization of surface receptors, and recycling to the cell surface, which determines the ability of a T cell to become activated. Increased recycling of CD3 and CD4 receptors occurs in lupus T cells, and could represent a mechanism by which T cells are sensitized to stimulation. This chapter explains methods used to investigate endocytic recycling of the TCR, CD4, and CD8 co-receptors in peripheral blood lymphocytes, T cells, and in splenocytes from lupus-prone murine models. The assays described will allow the study of surface receptor turnover in live untouched lymphocytes by flow cytometry.
authors.affiliation: Department of Medicine, College of Medicine, State University of New York, Upstate Medical University, Syracuse, NY, USA.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Telarico
authors.forename: Andras
authors.initials: A
authors.lastname: Perl
mesh_terms: D000818:Animals; D015551:Autoimmunity / Q000276:immunology*; D015153:Blotting, Western; D002469:Cell Separation; D003584:Cytological Techniques / Q000379:methods*; D004705:Endocytosis; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism; D013154:Spleen / Q000166:cytology
chemical_list: D011956:Receptors, Cell Surface