title: Development of chromosome-arm-specific microsatellite markers in Triticum aestivum (Poaceae) using NGS technology.
abstract: OBJECTIVE
  The aim of this study was to assess the feasibility of developing chromosome-arm-specific microsatellite markers in wheat on a large scale based on chromosome survey sequences obtained with next-generation sequencing (NGS) technology.
  
  
  RESULTS
  The Illumina Hi Seq2000 sequencing platform was used to sequence DNA of isolated wheat chromosome-arm 7DL. The data were assembled and microsatellite loci were identified computationally. In total, 16315 microsatellites were identified from 161061 assembled contigs. Thirty-three markers were randomly selected for validation across 20 diverse wheat cultivars. Two nulli-tetrasomic stocks were also screened to validate the specificity of the newly developed markers.
  
  
  CONCLUSIONS
  This is the first study on identification of chromosome-arm-specific microsatellite markers using NGS technology. These new chromosome-arm-specific markers will facilitate saturation of the 7DL genetic map, and their availability will support genetic mapping and positional cloning in wheat.
authors.affiliation: State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Nie
authors.forename: Bianli
authors.initials: B
authors.lastname: Li
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.forename: Peixun
authors.initials: P
authors.lastname: Liu
authors.forename: Siddanagouda S
authors.initials: SS
authors.lastname: Biradar
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Dolezel
authors.forename: David
authors.initials: D
authors.lastname: Edwards
authors.forename: Mingcheng
authors.initials: M
authors.lastname: Luo
authors.forename: Song
authors.initials: S
authors.lastname: Weining
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*; D014908:Triticum / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers