title: Adaptation by western corn rootworm (Coleoptera: Chrysomelidae) to Bt maize: inheritance, fitness costs, and feeding preference.
abstract: We examined inheritance of resistance, feeding behavior, and fitness costs for a laboratory-selected strain of western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), with resistance to maize (Zea maize L.) producing the Bacillus thuringiensis Berliner (Bt) toxin Cry3Bb1. The resistant strain developed faster and had increased survival on Bt maize relative to a susceptible strain. Results from reciprocal crosses of the resistant and susceptible strains indicated that inheritance of resistance was nonrecessive. No fitness costs were associated with resistance alleles in the presence of two entomopathogenic nematode species, Steinernema carpocapsae Weiser and Heterorhabditis bacteriophora Poinar. Larval feeding studies indicated that the susceptible and resistant strains did not differ in preference for Bt and non-Bt root tissue in choice assays.
authors.affiliation: Department of Entomology, Iowa State University, Ames, IA 50011, USA. jpetzold@iastate.edu
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Petzold-Maxwell
authors.forename: Ximena
authors.initials: X
authors.lastname: Cibils-Stewart
authors.forename: B Wade
authors.initials: BW
authors.lastname: French
authors.forename: Aaron J
authors.initials: AJ
authors.lastname: Gassmann
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001517:Coleoptera / Q000235:genetics*; D004731:Endotoxins; D005260:Female; D005518:Food Preferences; D006460:Hemolysin Proteins; D007305:Insecticide Resistance / Q000235:genetics; D007814:Larva; D008297:Male; D010572:Pest Control, Biological; D017168:Rhabditida
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; C030956:insecticidal crystal protein, Bacillus Thuringiensis