title: Cereal β-glucan quantification with calcofluor-application to cell culture supernatants.
abstract: The specific binding of the fluorescent dye calcofluor to cereal β-glucan results in increased fluorescence intensity of the formed complex and is in use for the quantification of β-glucan above a critical molecular weight (MW) by flow injection analysis. In this study, this method was applied in a fast and easy batch mode. In order to emphasize the spectral information of the emission spectra of the calcofluor/β-glucan complexes, derivative signals were calculated. A linear relationship was found between the amplitude of the second derivative signals and the β-glucan concentration between 0.1 and 0.4 μg/mL. The low detection limit of this new method (0.045 μg/mL) enabled its use to study the transport of cereal β-glucans over differentiated Caco-2 cell monolayers. Additionally, the method was applied to quantify β-glucan in arabinoxylan samples, which correlated well with data by an enzyme based method.
authors.affiliation: Nofima AS, Norwegian Institute of Food, Fishery and Aquaculture Research, Osloveien 1, 1430 Ås, Norway. anne.rieder@nofima.no
authors.forename: Anne
authors.initials: A
authors.lastname: Rieder
authors.forename: Svein H
authors.initials: SH
authors.lastname: Knutsen
authors.forename: Simon
authors.initials: S
authors.lastname: Ballance
authors.forename: Stine
authors.initials: S
authors.lastname: Grimmer
authors.forename: Diego
authors.initials: D
authors.lastname: Airado-Rodríguez
mesh_terms: D001557:Benzenesulfonates / Q000378:metabolism*; D018938:Caco-2 Cells; D002138:Calibration; D002523:Edible Grain / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D014990:Xylans / Q000378:metabolism*; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D001557:Benzenesulfonates; D005456:Fluorescent Dyes; D014990:Xylans; D047071:beta-Glucans; C007061:C.I. Fluorescent Brightening Agent 28; C085118:arabinoxylan