title: Exogenous long-term treatment with 17β-oestradiol alters the innervation pattern in pig ovary.
abstract: The aim of the present study was to determine the effect of long-term 17β-oestradiol (E2) exposure, a simulation of pathological states that occur with oestrogen overproduction, on the innervation patterns of ovaries in adult gilts. The intraovarian distribution and density of nerve fibres immunoreactive (IR) to protein gene product (PGP) 9.5 and containing dopamine-β-hydroxylase (DBH), neuropeptide Y (NPY), somatostatin (SOM) and galanin (GAL) were determined. From Day 4 of the first oestrous cycle to Day 20 of the second cycle studied, experimental gilts were injected with E2 (1000μg every 12h) whereas control gilts were injected with corn oil. After E2 administration, there was an increase in the number of PGP9.5-, DBH-, NPY- and GAL-IR fibres. Numerous PGP9.5-IR terminals were observed within the ground plexus around secondary follicles and small or medium tertiary follicles. Long-term E2 treatment increased the density of DBH- and NPY-IR fibres in the cortical part of the ground plexus, DBH- and GAL-IR fibres in the medullary part of the ground plexus, DBH-IR fibres near small and medium tertiary follicles and NPY-IR fibres around medullary arteries. The data indicate that long-term exposure of gilts to E2 increases the total number of intraovarian fibres, including sympathetic fibres. These results suggest that elevated E2 levels that occur during pathological states may affect the innervation patterns of ovaries and their function(s).
authors.affiliation: Division of Biology Reproduction, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Olsztyn 10-747, Tuwima 10, Poland.
authors.forename: Marlena
authors.initials: M
authors.lastname: Koszykowska
authors.forename: Jarosław
authors.initials: J
authors.lastname: Całka
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Nidzgorska
authors.forename: Barbara
authors.initials: B
authors.lastname: Jana
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D004958:Estradiol / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D004970:Estrone / Q000097:blood; D005260:Female; D019004:Galanin / Q000378:metabolism; D006652:Histological Techniques; D007150:Immunohistochemistry; D009412:Nerve Fibers / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism; D009929:Organ Size / Q000502:physiology; D010053:Ovary / Q000187:drug effects / Q000294:innervation*; D011374:Progesterone / Q000097:blood; D013004:Somatostatin / Q000378:metabolism; D013552:Swine
chemical_list: D009478:Neuropeptide Y; D004970:Estrone; D011374:Progesterone; D004958:Estradiol; D013004:Somatostatin; D019004:Galanin; D004299:Dopamine beta-Hydroxylase