title: Leishmania spp. epidemiology of canine leishmaniasis in the Yucatan Peninsula.
abstract: Canine Leishmaniasis is widespread in various Mexican states, where different species of Leishmania have been isolated from dogs. In the present study, we describe the detection of L. braziliensis, L. infantum, and L. mexicana in serum of dogs from the states of Yucatan and Quintana Roo in the Yucatan Peninsula (Mexico). A total of 412 sera were analyzed by ELISA using the total extract of the parasite and the iron superoxide dismutase excreted by different trypanosomatids as antigens. We found the prevalence of L. braziliensis to be 7.52%, L. infantum to be 6.07%, and L. mexicana to be 20.63%, in the dog population studied. The results obtained with ELISA using iron superoxide dismutase as the antigen were confirmed by western blot analysis with its greater sensitivity, and the agreement between the two techniques was very high.
authors.affiliation: Departamento de Parasitología, Facultad de Ciencias, Universidad de Granada, Severo Ochoa s/n, Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: López-Céspedes
authors.forename: S S
authors.initials: SS
authors.lastname: Longoni
authors.forename: C H
authors.initials: CH
authors.lastname: Sauri-Arceo
authors.forename: M
authors.initials: M
authors.lastname: Sánchez-Moreno
authors.forename: R I
authors.initials: RI
authors.lastname: Rodríguez-Vivas
authors.forename: F J
authors.initials: FJ
authors.lastname: Escobedo-Ortegón
authors.forename: M A
authors.initials: MA
authors.lastname: Barrera-Pérez
authors.forename: M E
authors.initials: ME
authors.lastname: Bolio-González
authors.forename: C
authors.initials: C
authors.lastname: Marín
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000097:blood*; D015153:Blotting, Western; D060085:Coinfection / Q000453:epidemiology / Q000469:parasitology; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007891:Leishmania / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity*; D007896:Leishmaniasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008800:Mexico / Q000453:epidemiology; D015995:Prevalence; D012680:Sensitivity and Specificity; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D013482:Superoxide Dismutase