title: Effects of degree of carboxymethylation on physicochemical and biological properties of pachyman.
abstract: Polysaccharides (pachyman) extracted from Poria cocos sclerotium were chemically modified by carboxymethylation and the effects on the structural and biological properties of the polysaccharides were investigated as a function of the degree of carboxymethylation. The degree of substitution (DS) of five carboxymethylated pachyman, coded as CMP1/CMP2/CMP3/CMP4/CMP5, was determined to be 0.44-0.88. The structures were confirmed by FT-IR and their weight-average molecular masses (Mw) were obtained by SEC-LLS. Experimental results showed that derivatives were effective in anti-oxidation and bile acid binding in a dose dependent way. Furthermore, their water solubility and biological activities were improved with the increase of DS. Therefore, results proved that the carboxymethylation of pachyman effectively enhanced their potential biological properties.
authors.affiliation: Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, PR China. river0301@163.com
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Mo
authors.forename: Zenan
authors.initials: Z
authors.lastname: Li
authors.forename: Hongwu
authors.initials: H
authors.lastname: Lai
authors.forename: Jian
authors.initials: J
authors.lastname: Lou
authors.forename: Shiwang
authors.initials: S
authors.lastname: Liu
authors.forename: Jianwei
authors.initials: J
authors.lastname: Mao
mesh_terms: D001647:Bile Acids and Salts / Q000378:metabolism; D002614:Chelating Agents / Q000737:chemistry* / Q000378:metabolism*; D055598:Chemical Phenomena; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000378:metabolism*; D005609:Free Radicals / Q000737:chemistry; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D008970:Molecular Weight; D012995:Solubility; D013329:Structure-Activity Relationship; D013481:Superoxides / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D001647:Bile Acids and Salts; D002614:Chelating Agents; D016166:Free Radical Scavengers; D005609:Free Radicals; D005936:Glucans; C568908:carboxymethylpachymaran; D014867:Water; D013481:Superoxides; D017665:Hydroxyl Radical; D007501:Iron