title: Gentamicin in combination with ascorbic acid regulates the severity of Staphylococcus aureus infection-induced septic arthritis in mice.
abstract: To study the effects of gentamicin in combination with ascorbic acid on septic arthritis, mice were infected with Staphylococcus aureus (S. aureus) and treated with gentamicin, which was given at 5 mg/kg after 24 h of infection, followed by ascorbic acid, given at 20 mg/kg body weight after 2 h of gentamicin treatment. Mice were sacrificed at 3, 9, 15 days post-infection (dpi). Combined treatment of infected mice with gentamicin and ascorbic acid eradicated the bacteria from the blood, spleen and synovial tissue and showed a significant gross reduction in arthritis, reduced serum levels of tumour necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ). S. aureus-infected mice have demonstrated the disturbed antioxidant status measured in terms of cellular antioxidants like reduced glutathione and antioxidant enzymes such as superoxide dismutase (SOD) and catalase. The same were ameliorated when the animals were co-treated with gentamicin along with ascorbic acid.
authors.affiliation: Department of Physiology, Immunology Laboratory, University of Calcutta, University Colleges of Science and Technology, Calcutta, West Bengal, India.
authors.forename: P
authors.initials: P
authors.lastname: Mal
authors.forename: S
authors.initials: S
authors.lastname: Dutta
authors.forename: D
authors.initials: D
authors.lastname: Bandyopadhyay
authors.forename: K
authors.initials: K
authors.lastname: Dutta
authors.forename: A
authors.initials: A
authors.lastname: Basu
authors.forename: B
authors.initials: B
authors.lastname: Bishayi
mesh_terms: D000818:Animals; D001170:Arthritis, Infectious / Q000188:drug therapy* / Q000209:etiology / Q000276:immunology; D001205:Ascorbic Acid / Q000008:administration & dosage*; D002478:Cells, Cultured; D018450:Disease Progression; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005839:Gentamicins / Q000008:administration & dosage*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D013203:Staphylococcal Infections / Q000150:complications / Q000188:drug therapy* / Q000276:immunology; D013211:Staphylococcus aureus / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D005839:Gentamicins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D013482:Superoxide Dismutase; D001205:Ascorbic Acid