title: Phage display-based strategies for cloning and optimization of monoclonal antibodies directed against human pathogens.
abstract: In the last two decades, several phage display-selected monoclonal antibodies (mAbs) have been described in the literature and a few of them have managed to reach the clinics. Among these, the anti-respiratory syncytial virus (RSV) Palivizumab, a phage-display optimized mAb, is the only marketed mAb directed against microbial pathogens. Palivizumab is a clear example of the importance of choosing the most appropriate strategy when selecting or optimizing an anti-infectious mAb. From this perspective, the extreme versatility of phage-display technology makes it a useful tool when setting up different strategies for the selection of mAbs directed against human pathogens, especially when their possible clinical use is considered. In this paper, we review the principal phage display strategies used to select anti-infectious mAbs, with particular attention focused on those used against hypervariable pathogens, such as HCV and influenza viruses.
authors.affiliation: Microbiology and Virology Unit, "Vita-Salute" San Raffaele University, Milan 20132, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Clementi
authors.affiliation: Microbiology and Virology Unit, "Vita-Salute" San Raffaele University, Milan 20132, Italy.
authors.forename: Nicasio
authors.initials: N
authors.lastname: Mancini
authors.affiliation: Microbiology and Virology Unit, "Vita-Salute" San Raffaele University, Milan 20132, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Solforosi
authors.affiliation: Microbiology and Virology Unit, "Vita-Salute" San Raffaele University, Milan 20132, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Castelli
authors.affiliation: Microbiology and Virology Unit, "Vita-Salute" San Raffaele University, Milan 20132, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Clementi
authors.affiliation: Microbiology and Virology Unit, "Vita-Salute" San Raffaele University, Milan 20132, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Burioni
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D001402:B-Lymphocytes / Q000502:physiology; D003001:Cloning, Molecular; D016174:Hepacivirus / Q000187:drug effects / Q000276:immunology; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects / Q000276:immunology; D019151:Peptide Library; D012107:Research Design
chemical_list: D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D019151:Peptide Library