title: Real-time observation of antigen-antibody association using a low-cost biosensing system based on photonic bandgap structures.
abstract: In this letter, we present experimental results of antibody detection using a biosensor based on photonic bandgap structures, which are interrogated using a power-based readout technique. This interrogation method allows a real-time monitoring of the association process between the antigen probes and the target antibodies, allowing the instantaneous observation of any interaction event between molecules. because etunable lasers and optical spectrum analyzers are avoided for the readout, a drastic reduction of the final cost of the platform is obtained. Furthermore, the performance of the biosensing system is significantly enhanced due to the large number of data values obtained per second.
authors.affiliation: NanophotonicsTechnology Center, Universitat Politècnica de València, Valencia, Spain. jagarcas@ntc.upv.es
authors.forename: J G
authors.initials: JG
authors.lastname: Castelló
authors.forename: V
authors.initials: V
authors.lastname: Toccafondo
authors.forename: J
authors.initials: J
authors.lastname: Escorihuela
authors.forename: M J
authors.initials: MJ
authors.lastname: Bañuls
authors.forename: A
authors.initials: A
authors.lastname: Maquieira
authors.forename: J
authors.initials: J
authors.lastname: García-Rupérez
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D015374:Biosensing Techniques / Q000191:economics* / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D046210:Microfluidic Analytical Techniques; D017785:Photons; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000276:immunology*; D013997:Time Factors
chemical_list: D000906:Antibodies; D012710:Serum Albumin, Bovine