title: Vascular endothelial growth factor receptor-3 expression in mycosis fungoides.
abstract: Here, we have studied vascular endothelial growth factor receptor-3 (VEGFR-3) expression in mycosis fungoides (MF), the most common type of cutaneous T-cell lymphoma (CTCL). Immunohistochemistry revealed that in two-thirds of 34 patients, VEGFR-3 was expressed in situ by both tumor and stromal cells irrespective of the disease stage. The natural VEGFR-3 ligand, VEGF-C, partially protected malignant T-cell lines from growth inhibition by the histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA). Whereas the malignant T cells did not produce VEGF-C in vitro, its expression was induced during tumor formation in vivo in a xenograft mouse model of MF. In conclusion, malignant and stromal cells express high levels of VEGFR-3 in all stages of MF. Moreover, malignant T cells trigger enhanced VEGF-C expression in fibroblasts, suggesting that cross-talk between tumor and stromal cells plays a role in lymphangiogenesis and possibly disease progression.
authors.affiliation: Department of International Health, Immunology and Microbiology, University of Copenhagen, Denmark.
authors.forename: Ida Holst
authors.initials: IH
authors.lastname: Pedersen
authors.forename: Andreas
authors.initials: A
authors.lastname: Willerslev-Olsen
authors.forename: Claudia
authors.initials: C
authors.lastname: Vetter-Kauczok
authors.forename: Thorbjørn
authors.initials: T
authors.lastname: Krejsgaard
authors.forename: Britt
authors.initials: B
authors.lastname: Lauenborg
authors.forename: Katharina Luise
authors.initials: KL
authors.lastname: Kopp
authors.forename: Carsten
authors.initials: C
authors.lastname: Geisler
authors.forename: Charlotte M
authors.initials: CM
authors.lastname: Bonefeld
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Mariusz A
authors.initials: MA
authors.lastname: Wasik
authors.forename: Sally
authors.initials: S
authors.lastname: Dabelsteen
authors.forename: Anders
authors.initials: A
authors.lastname: Woetmann
authors.forename: Jurgen C
authors.initials: JC
authors.lastname: Becker
authors.forename: Niels
authors.initials: N
authors.lastname: Odum
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D015870:Gene Expression; D006801:Humans; D051379:Mice; D009182:Mycosis Fungoides / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012878:Skin Neoplasms / Q000235:genetics* / Q000378:metabolism; D014183:Transplantation, Heterologous; D042582:Vascular Endothelial Growth Factor C / Q000235:genetics / Q000378:metabolism; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D042582:Vascular Endothelial Growth Factor C; D040321:Vascular Endothelial Growth Factor Receptor-3