title: Echinacoside promotes bone regeneration by increasing OPG/RANKL ratio in MC3T3-E1 cells.
abstract: Echinacoside (ECH), isolated from Cistanche tubulosa (Schrenk) R. Wight stems, was subjected to in vitro experiments to investigate its bioactivities on proliferation, differentiation and mineralization of the osteoblastic cell line MC3T3-E1. MTT assay, the alkaline phosphatase (ALP) activity and calcium deposition were determined, and the secretion of collagen I (COL I), osteocalcin (OCN), osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) were also assayed by enzyme-linked immunosorbent assay (ELISA). The results showed that ECH caused a significant increase in cell proliferation, ALP activity, COL I contents, OCN levels and an enhancement of mineralization in osteoblasts at the concentration range from 0.01 to 10nmol·L(-1) (p<0.05), suggesting that ECH has a stimulatory effect on osteoblastic bone formation or has potential activity against osteoporosis. In addition, the ratio of OPG/RANKL also could be enhanced by ECH. These findings provide the potent evidence that ECH can promote bone regeneration in cultured osteoblastic MC3T3-E1 cells, which might be done by elevating the OPG/RANKL ratio, and potential evidence for echinacoside to be a promising drug or a lead compound in the development of disease-modifying drug to prevent osteoporosis.
authors.affiliation: State Key Laboratory of Natural Products and Functions, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yang
authors.forename: Panpan
authors.initials: P
authors.lastname: Zhu
authors.forename: Weina
authors.initials: W
authors.lastname: Chen
authors.forename: Dongli
authors.initials: D
authors.lastname: Qi
authors.forename: Xiupu
authors.initials: X
authors.lastname: Shi
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D002460:Cell Line; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects*; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism*; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism*
chemical_list: D024042:Collagen Type I; D006027:Glycosides; D053244:Osteoprotegerin; D053245:RANK Ligand; C506031:Tnfrsf11b protein, mouse; C505689:Tnfsf11 protein, mouse; D015675:Osteocalcin; D000469:Alkaline Phosphatase; C060297:echinacoside