title: Colonization of the satellite cell niche by skeletal muscle progenitor cells depends on Notch signals.
abstract: Skeletal muscle growth and regeneration rely on myogenic progenitor and satellite cells, the stem cells of postnatal muscle. Elimination of Notch signals during mouse development results in premature differentiation of myogenic progenitors and formation of very small muscle groups. Here we show that this drastic effect is rescued by mutation of the muscle differentiation factor MyoD. However, rescued myogenic progenitors do not assume a satellite cell position and contribute poorly to myofiber growth. The disrupted homing is due to a deficit in basal lamina assembly around emerging satellite cells and to their impaired adhesion to myofibers. On a molecular level, emerging satellite cells deregulate the expression of basal lamina components and adhesion molecules like integrin α7, collagen XVIIIα1, Megf10, and Mcam. We conclude that Notch signals control homing of satellite cells, stimulating them to contribute to their own microenvironment and to adhere to myofibers.
authors.affiliation: Developmental Biology/Signal Transduction Group, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13125 Berlin, Germany.
authors.forename: Dominique
authors.initials: D
authors.lastname: Bröhl
authors.forename: Elena
authors.initials: E
authors.lastname: Vasyutina
authors.forename: Maciej T
authors.initials: MT
authors.lastname: Czajkowski
authors.forename: Joscha
authors.initials: J
authors.lastname: Griger
authors.forename: Claudia
authors.initials: C
authors.lastname: Rassek
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Rahn
authors.forename: Bettina
authors.initials: B
authors.lastname: Purfürst
authors.forename: Hagen
authors.initials: H
authors.lastname: Wende
authors.forename: Carmen
authors.initials: C
authors.lastname: Birchmeier
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D017570:MyoD Protein / Q000235:genetics / Q000378:metabolism; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D051880:Receptors, Notch