title: Lysozyme resistance of the ropy strain Pediococcus parvulus IOEB 8801 is correlated with beta-glucan accumulation around the cell.
abstract: Lactic acid bacteria (LAB) are often exploited to carry out malolactic fermentation in wine. However, a few specific LAB strains and, more precisely, some Pediococcus parvulus strains synthesize a β-glucan, which can be deleterious to wine quality as it confers a ropy texture to the wine that can no longer be commercialized. Although molecular methods exist to detect these unwanted microorganisms, ropy Pediococcus still remain difficult to remove from wine, because of their natural resistance to traditional wine stabilizing treatments. In this work, we show that ropy P. parvulus are resistant to lysozyme. We clearly demonstrate that this resistance may be ascribed to the presence of the β-glucan that forms around the cell a protective barrier against anti-bacteria agents. Moreover, this resistance increases during bacterial growth. We show that using lysozyme with β-glucanase can strongly improve the treatment against ropy strains, in model media as well as red and white wine based media. This work not only brings potential solutions to the wine industry, but also opens interesting perspectives for studying β-glucan producing bacteria which are widespread in the food industry.
authors.affiliation: Microflora, ISVV, 210 chemin de Leysotte, CS 50008-33882 Villenave d'Ornon cedex, France.
authors.forename: Joana
authors.initials: J
authors.lastname: Coulon
authors.forename: Anne
authors.initials: A
authors.lastname: Houlès
authors.forename: Maria
authors.initials: M
authors.lastname: Dimopoulou
authors.forename: Julie
authors.initials: J
authors.lastname: Maupeu
authors.forename: Marguerite
authors.initials: M
authors.lastname: Dols-Lafargue
mesh_terms: D001419:Bacteria; D005285:Fermentation; D007777:Lactobacillaceae; D009113:Muramidase / Q000378:metabolism*; D010376:Pediococcus / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D014920:Wine / Q000382:microbiology*; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D047071:beta-Glucans; D009113:Muramidase