title: Inhibition of Staphylococcus aureus adherence to Caco-2 cells by lactobacilli and cell surface properties that influence attachment.
abstract: Staphylococcus aureus is an opportunistic pathogen that can colonize human and animal intestinal tracts, causing certain gastrointestinal diseases. The adherence of enteric pathogens to host intestinal epithelial cells is important for their pathogenesis. In the present study, Lactobacillus salivarius and Lactobacillus plantarum were investigated in vitro to examine their ability to competitively exclude S. aureus. Various factors involved in attachment, including bacterial status and cell concentration, growth phase, competition patterns, and surface-layer protein extracts, were also investigated. Live lactobacilli in the mid-log growth phase exhibited maximum inhibitory activity when lactobacilli were pre- or co-incubated with S. aureus. However, the inhibitory activity was significantly reduced when the lactobacilli were inactivated by heating or treated with LiCl. Furthermore, both lactobacilli possessed certain cell surface properties, such as hydrophobicity, autoaggregation, and coaggregation ability. L. salivarius and L. plantarum strongly inhibited S. aureus adherence to Caco-2 cells and their inhibition activity was significantly influenced by several factors that affect adhesion inhibition.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, PR China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Ren
authors.forename: Chang
authors.initials: C
authors.lastname: Li
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Qin
authors.forename: Ronglan
authors.initials: R
authors.lastname: Yin
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.forename: Mingyao
authors.initials: M
authors.lastname: Tian
authors.forename: Shouwen
authors.initials: S
authors.lastname: Du
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Guo
authors.forename: Cunxia
authors.initials: C
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Zhu
authors.forename: Dandan
authors.initials: D
authors.lastname: Sun
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Ningyi
authors.initials: N
authors.lastname: Jin
mesh_terms: D000898:Antibiosis; D001422:Bacterial Adhesion; D018938:Caco-2 Cells; D002473:Cell Wall / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007778:Lactobacillus / Q000502:physiology*; D013211:Staphylococcus aureus / Q000472:pathogenicity*; D013499:Surface Properties