title: TFinDit: transcription factor-DNA interaction data depository.
abstract: BACKGROUND
  One of the crucial steps in regulation of gene expression is the binding of transcription factor(s) to specific DNA sequences. Knowledge of the binding affinity and specificity at a structural level between transcription factors and their target sites has important implications in our understanding of the mechanism of gene regulation. Due to their unique functions and binding specificity, there is a need for a transcription factor-specific, structure-based database and corresponding web service to facilitate structural bioinformatics studies of transcription factor-DNA interactions, such as development of knowledge-based interaction potential, transcription factor-DNA docking, binding induced conformational changes, and the thermodynamics of protein-DNA interactions.
  
  
  METHODS
  TFinDit is a relational database and a web search tool for studying transcription factor-DNA interactions. The database contains annotated transcription factor-DNA complex structures and related data, such as unbound protein structures, thermodynamic data, and binding sequences for the corresponding transcription factors in the complex structures. TFinDit also provides a user-friendly interface and allows users to either query individual entries or generate datasets through culling the database based on one or more search criteria.
  
  
  CONCLUSIONS
  TFinDit is a specialized structural database with annotated transcription factor-DNA complex structures and other preprocessed data. We believe that this database/web service can facilitate the development and testing of TF-DNA interaction potentials and TF-DNA docking algorithms, and the study of protein-DNA recognition mechanisms.
authors.affiliation: Department of Bioinformatics and Genomics, College of Computing and Informatics, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Turner
authors.forename: RyangGuk
authors.initials: R
authors.lastname: Kim
authors.forename: Jun-tao
authors.initials: JT
authors.lastname: Guo
mesh_terms: D000465:Algorithms; D001665:Binding Sites; D019295:Computational Biology; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D030541:Databases, Genetic; D020407:Internet; D012984:Software; D013816:Thermodynamics; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism
chemical_list: D014157:Transcription Factors; D004247:DNA