title: Isolation and characterization of microsatellite markers in brown planthopper (Nilaparvata lugens Stål).
abstract: Brown planthopper (Nilaparvata lugens Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from N. lugens genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Polymorphism of each locus was detected in 48 individuals from two natural populations. These microsatellite loci revealed 2 to 18 alleles, and the expected and observed heterozygosities ranged from 0.042 to 0.937 and from 0.042 to 0.958, respectively. These markers will be useful for the future study of this agricultural pest in population genetics and molecular genetics.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Jing
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Hangjin
authors.initials: H
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Bingfang
authors.initials: B
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, China.
authors.forename: Guangcun
authors.initials: G
authors.lastname: He
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D014644:Genetic Variation / Q000235:genetics*; D005828:Genetics, Population; D049750:Genome, Insect; D006430:Hemiptera / Q000145:classification* / Q000235:genetics*; D018895:Microsatellite Repeats