title: Genotoxicity studies of glycidol fatty acid ester (glycidol linoleate) and glycidol.
abstract: Glycidol fatty acid esters (GEs) are found in refined edible oils. Safety concerns have been alleged due to the possible release of glycidol (G), an animal carcinogen. We evaluated the genotoxic potential of glycidol linoleate (GL), a primary GE found in an edible oil (diacylglycerol oil), and G, using three established genotoxicity tests (a bacterial reverse mutation test, an in vitro chromosomal aberration test, and an in vivo bone marrow micronucleus test) under GLP conditions complying with all OECD guidelines. In the bacterial reverse mutation test, GL and G showed positive responses. The positive responses of GL were less than those of G and observed only in strains detecting point mutations where G showed remarkably positive responses. G was involved in the positive response of GL. In the chromosomal aberration test, GL did not induce chromosome aberrations whereas G induced structural chromosome aberrations in the presence and absence of metabolic activation. In the bone marrow micronucleus test, neither GL nor G induced significant increases of micronucleated immature (polychromatic) erythrocytes in bone marrow of test animals. Based on the above results as well as pertinent information on toxicokinetics, GL itself does not play a key role in genotoxic action.
authors.affiliation: Global R&D Safety Science, Kao Corporation, 2606 Akabane, Ichikai-Machi, Haga-Gun, Tochigi 321-3497, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Ikeda
authors.forename: Kenkichi
authors.initials: K
authors.lastname: Fujii
authors.forename: Miko
authors.initials: M
authors.lastname: Sarada
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Saito
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Kawabata
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Naruse
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Yuki
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakagiri
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Honda
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Tamaki
authors.forename: Naohiro
authors.initials: N
authors.lastname: Nishiyama
authors.forename: Toshio
authors.initials: T
authors.lastname: Kasamatsu
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D004852:Epoxy Compounds / Q000633:toxicity*; D008041:Linoleic Acids / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests / Q000379:methods; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation; D020005:Propanols / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000235:genetics
chemical_list: D004852:Epoxy Compounds; D008041:Linoleic Acids; D020005:Propanols; C576812:glycidol linoleate; C004312:glycidol