title: [Preparation and in vivo-in vitro evaluation of compound nanoparticles loaded with epirubicin hydrochloride and gadopentetate meglumine].
abstract: OBJECTIVE
  To prepare and characterize the compound Epirubicin hydrochloride and Gadopentetate meglumine (Gd-DTPA) nanoparticles, and evaluate its properties from rabbits in vivo and in vitro.
  
  
  METHODS
  The compound Epirubicin hydrochloride and Gd-DTPA nanoparticles were prepared by double emulsion-solvent evaporation method. The main effective factors were orthogonal designed. The characteristics such as drug entrapment efficiency, drug loading, and drug utilization were assayed in vitro. MR imaging effect of the VX2 rabbit hepatoma model were observed after injecting the drug-loaded nanoparticles through the hepatic artery intubation in vivo.
  
  
  RESULTS
  The drug encapsulation efficiency of the nanoparticles, drug loading and drug utilization were 33.8% ± 3.4%, 0.225% ± 0.052%, and 69.6% ± 4.3% under the optimized prescription, respectively. The mean size of the nanoparticles was 180.6 nm, the drug release continued in 48 h with good MR imaging effect.
  
  
  CONCLUSIONS
  Compound Epirubicin hydrochloride and Gd-DTPA Nanoparticles were in simply preparation and showed sustained drug release properties. These novel nanoparticles with detecting function could develop of epirubicin hydrochloride targeted therapy of liver cancer.
authors.affiliation: Department of Radiology, Zhongda Hospital of Southeast University, Nanjing 210009, China.
authors.forename: Jian-song
authors.initials: JS
authors.lastname: Ji
authors.forename: Gao-jun
authors.initials: GJ
authors.lastname: Teng
authors.forename: Hong-yuan
authors.initials: HY
authors.lastname: Yang
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Song
authors.forename: Chen-ying
authors.initials: CY
authors.lastname: Lu
authors.forename: Hong-xing
authors.initials: HX
authors.lastname: Fu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004337:Drug Carriers; D004339:Drug Compounding / Q000379:methods*; D015251:Epirubicin / Q000138:chemical synthesis* / Q000494:pharmacology*; D019786:Gadolinium DTPA; D053758:Nanoparticles; D010316:Particle Size; D011817:Rabbits
chemical_list: D004337:Drug Carriers; D015251:Epirubicin; D019786:Gadolinium DTPA