title: Purification and antitumor activity of two acidic polysaccharides from the roots of Polygala tenuifolia.
abstract: Two acidic polysaccharide fractions (PTPa and PTPb) extracted from the roots of Polygala tenuifolia, were obtained by DEAE-Sephacel anion-exchange, and Sephadex G-100 gel-permeation chromatography. High-performance liquid chromatography (HPLC) identified that PTPa and PTPb was composed of Ara, Glc, Gal, Man and GlcUA in the proportion of 2.4:1.2:0.6:0.4:1.1 and 2.1:1.7:0.5:0.6:1.7, respectively. Their molecular weight was evaluated to be 5.9×10(4) (PTPa) and 2.5×10(4) Da (PTPb) as determined by high performance size exclusion chromatography (HPSEC). Pharmacological studies revealed PTPa and PTPb significantly inhibited the growth of A549 cells in vitro and exhibited significantly higher antitumor activity against solid tumor A549 in vivo than did a blank control. Moreover, treatment with two acidic polysaccharides caused an enhancement of superoxide dismutase (SOD) and catalase (CAT) activities in tumor-bearing mice and a reduction in thiobarbituric acid reactive substances (TBARS) level. Taken together, these results indicated that two acidic polysaccharides from the roots of P. tenuifolia may be useful as potent antitumor agents for the prevention of lung tumorigenesis.
authors.affiliation: Department of Medical Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin 150081, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xin
authors.forename: Fubin
authors.initials: F
authors.lastname: Zhang
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Jiang
authors.forename: Chunhong
authors.initials: C
authors.lastname: Chen
authors.forename: Dayong
authors.initials: D
authors.lastname: Huang
authors.forename: Yanju
authors.initials: Y
authors.lastname: Lv
authors.forename: Weixi
authors.initials: W
authors.lastname: Shen
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000143:Acids / Q000737:chemistry; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000143:Acids; D000970:Antineoplastic Agents; D000975:Antioxidants; D011134:Polysaccharides; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D013482:Superoxide Dismutase