title: The structure of the O-specific polysaccharide of the lipopolysaccharide from Yersinia enterocolitica serotype O:50 strain 3229.
abstract: The genus Yersinia represents Gram-negative bacteria that are widely distributed in the environment and possess lipopolysaccharide (LPS) as a major molecule on the surface of the bacterial envelope. LPS is composed of an anchoring lipid, termed lipid A, and a polysaccharide part which not only determines the bacterial serotype but may also serve as a bacteriophage receptor. Here we present the structure of the O-polysaccharide (O-PS) of Yersinia enterocolitica O:50 phage ΦR1-37-sensitive strain 3229. The structural characterization was performed utilizing 1D and 2D nuclear magnetic resonance spectroscopy and chemical analyses. The structure of the O-PS biological repeating unit was identified as →2)-α-L-Rhap-(1→3)-α-L-FucpNAc-(1→3)-α-L-FucpNAc-(1→3)-β-D-GlcpNAc-(1→.
authors.affiliation: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany. abeczala@fz-borstel.de
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Beczała
authors.forename: Katarzyna Anna
authors.initials: KA
authors.lastname: Duda
authors.forename: Mikael
authors.initials: M
authors.lastname: Skurnik
authors.forename: Otto
authors.initials: O
authors.lastname: Holst
mesh_terms: D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification; D015008:Yersinia enterocolitica / Q000737:chemistry*
chemical_list: D019081:O Antigens