title: Effects of purified Saccharomyces cerevisiae (1→3)-β-glucan on venous ulcer healing.
abstract: Water-insoluble glucan was isolated from the baker's yeast Saccharomyces cerevisiae. The yeast cells were treated with alkali and the residue then with acid. Chemical and NMR (1D and 2D) analyses showed that a linear (1→3)-β-glucan was purified that was not contaminated with other carbohydrates, proteins or phenolic compounds. The effects of the glucan on wound healing were assessed in human venous ulcers by histopathological analysis after 30 days of topical treatment. (1→3)-β-glucan enhanced ulcer healing and increased epithelial hyperplasia, as well as increased inflammatory cells, angiogenesis and fibroblast proliferation. In one patient who had an ulcer that would not heal for over 15 years, glucan treatment caused a 67.8% decrease in the area of the ulcer. This is the first study to investigate the effects of (1→3)-β-glucan on venous ulcer healing in humans; our findings suggest that this glucan is a potential natural biological response modifier in wound healing.
authors.affiliation: Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil.
authors.forename: Sarah Dantas Viana
authors.initials: SDV
authors.lastname: Medeiros
authors.affiliation: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), 3000, Salgado Filho Ave., Natal, RN 59078-970, Brazil.
authors.forename: Sara Lima
authors.initials: SL
authors.lastname: Cordeiro
authors.affiliation: Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil.
authors.forename: Jéssica Escorel Chaves
authors.initials: JEC
authors.lastname: Cavalcanti
authors.affiliation: Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil.
authors.forename: Karina Mendes
authors.initials: KM
authors.lastname: Melchuna
authors.affiliation: Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil.
authors.forename: Aleida Maria da Silva
authors.initials: AMDS
authors.lastname: Lima
authors.affiliation: University Hospital Onofre Lopes, Department of Surgery, Federal University of Rio Grande do Norte (UFRN), 620, Nilo Peçanha st., Petrópolis, Natal, RN 59012-300, Brazil.
authors.forename: Irami Araújo
authors.initials: IA
authors.lastname: Filho
authors.affiliation: University Hospital Onofre Lopes, Department of Surgery, Federal University of Rio Grande do Norte (UFRN), 620, Nilo Peçanha st., Petrópolis, Natal, RN 59012-300, Brazil.
authors.forename: Aldo Cunha
authors.initials: AC
authors.lastname: Medeiros
authors.affiliation: Laboratory of Pathology, Department of Pathology, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil.
authors.forename: Keyla Borges Ferreira
authors.initials: KBF
authors.lastname: Rocha
authors.affiliation: Laboratory of Pathology, Department of Pathology, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil.
authors.forename: Elizabeth Maia
authors.initials: EM
authors.lastname: Oliveira
authors.affiliation: University Hospital Onofre Lopes, Department of Integrated Medicine, Federal University of Rio Grande do Norte (UFRN), 620, Nilo Peçanha st., Petrópolis, Natal, RN 59012-300, Brazil.
authors.forename: Eduardo Dantas Baptista
authors.initials: EDB
authors.lastname: Faria
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Paraná (UFPR), Cento Politécnico S/N, Jardim das Américas, Curitiba, PR 81531-990, Brazil.
authors.forename: Guilherme Lanzi
authors.initials: GL
authors.lastname: Sassaki
authors.affiliation: Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), 3000, Salgado Filho Ave., Natal, RN 59078-970, Brazil.
authors.forename: Hugo Alexandre Oliveira
authors.initials: HAO
authors.lastname: Rocha
authors.affiliation: Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil.
authors.forename: Valéria Soraya Farias
authors.initials: VSF
authors.lastname: Sales
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D062610:Fungal Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005936:Glucans / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D014647:Varicose Ulcer / Q000188:drug therapy*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D062610:Fungal Polysaccharides; D005936:Glucans