title: Mitochondrial mutation impairs cytoplasmic male sterility rice in response to H₂O₂ stress.
abstract: Cytoplasmic male sterility (CMS) is a phenomenon widely observed in various plant species characterized with disrupted anther development caused by mitochondrial mutation. CMS is becoming a model system for the investigations of nucleus-cytoplasmic interaction. To reveal the possible effects of CMS genes on plant growth in adverse environment, plant development and biochemical characters of mitochondria from Honglian (HL)-CMS line Yuetai A and maintainer Yuetai B treated with H(2)O(2) were analyzed. Results showed that 40-60mM H(2)O(2) significantly inhibits rice seedling development and growth. When treated with H(2)O(2), ATP content and mitochondrial membrane potential in Yuetai A decreased significantly faster than those of Yuetai B. These biochemical changes were accompanied by the severe nuclear DNA fragmentation and the release of mitochondrial cytochrome c in the leaf cells of Yuetai A. In addition, the antioxidative enzyme activities and mitochondrial electron transfer chain complexes were significantly down-regulated. Disturbance of the biochemical indexes indicate that HL-CMS line is more susceptible to H(2)O(2) stress than the maintainer line, the deleterious effects caused by the CMS-related ORFH79 peptide compromises the adaptability of HL-CMS line to the adverse environment.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Li
authors.forename: Cuixiang
authors.initials: C
authors.lastname: Wan
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Hu
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Yingguo
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000975:Antioxidants / Q000378:metabolism; D002467:Cell Nucleus; D045304:Cytochromes c / Q000378:metabolism; D003593:Cytoplasm; D053938:DNA Fragmentation; D015536:Down-Regulation; D004579:Electron Transport / Q000235:genetics; D035264:Flowers / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D050259:Genes, Mitochondrial; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000235:genetics; D008928:Mitochondria / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D009154:Mutation; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D010455:Peptides / Q000235:genetics; D063245:Plant Development / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D051479:Plant Infertility / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects; D010940:Plant Proteins / Q000235:genetics; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D000975:Antioxidants; D010455:Peptides; D010940:Plant Proteins; D000255:Adenosine Triphosphate; D045304:Cytochromes c; D006861:Hydrogen Peroxide