title: Enrichment and purification of syringin, eleutheroside E and isofraxidin from Acanthopanax senticosus by macroporous resin.
abstract: In order to screen a suitable resin for the preparative simultaneous separation and purification of syringin, eleutheroside E and isofraxidin from Acanthopanax senticosus, the adsorption and desorption properties of 17 widely used commercial macroporous resins were evaluated. According to our results, HPD100C, which adsorbs by the molecular tiers model, was the best macroporous resin, offering higher adsorption and desorption capacities and higher adsorption speed for syringin, eleutheroside E and isofraxidin than other resins. Dynamic adsorption and desorption tests were carried out to optimize the process parameters. The optimal conditions were as follows: for adsorption, processing volume: 24 BV, flow rate: 2 BV/h; for desorption, ethanol-water solution: 60:40 (v/v), eluent volume: 4 BV, flow rate: 3 BV/h. Under the above conditions, the contents of syringin, eleutheroside E and isofraxidin increased 174-fold, 20-fold and 5-fold and their recoveries were 80.93%, 93.97% and 93.79%, respectively.
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China.
authors.forename: Fengjian
authors.initials: F
authors.lastname: Yang
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China.
authors.forename: Chunjian
authors.identifier: 0000-0003-2827-1999
authors.initials: C
authors.lastname: Zhao
authors.affiliation: State Engineering Laboratory for Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, China.
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
mesh_terms: D000327:Adsorption; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification*; D028001:Eleutherococcus / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification*; D010666:Phenylpropionates / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D003374:Coumarins; D005960:Glucosides; D017705:Lignans; D010666:Phenylpropionates; C008182:isofraxidin; C421885:eleutheroside E; C028305:syringin