title: Intercellular carbon nanotube translocation assessed by flow cytometry imaging.
abstract: The fate of carbon nanotubes in the organism is still controversial. Here, we propose a statistical high-throughput imaging method to localize and quantify functionalized multiwalled carbon nanotubes in cells. We give the first experimental evidence of an intercellular translocation of carbon nanotubes. This stress-induced longitudinal transfer of nanomaterials is mediated by cell-released microvesicles known as vectors for intercellular communication. This finding raises new critical issues for nanotoxicology, since carbon nanotubes could be disseminated by circulating extracellular cell-released vesicles and visiting several cells in the course of their passage into the organism.
authors.affiliation: CNRS/Université Paris Diderot, PRES Sorbonne-Paris Cité, Laboratoire Matière et Systèmes Complexes (MSC), UMR7057, 75205 Paris cedex 13, France.
authors.forename: Iris
authors.initials: I
authors.lastname: Marangon
authors.forename: Nicole
authors.initials: N
authors.lastname: Boggetto
authors.forename: Cécilia
authors.initials: C
authors.lastname: Ménard-Moyon
authors.forename: Enrica
authors.initials: E
authors.lastname: Venturelli
authors.forename: Marie-Lys
authors.initials: ML
authors.lastname: Béoutis
authors.forename: Christine
authors.initials: C
authors.lastname: Péchoux
authors.forename: Nathalie
authors.initials: N
authors.lastname: Luciani
authors.forename: Claire
authors.initials: C
authors.lastname: Wilhelm
authors.forename: Alberto
authors.initials: A
authors.lastname: Bianco
authors.forename: Florence
authors.initials: F
authors.lastname: Gazeau
mesh_terms: D002478:Cells, Cultured; D004058:Diffusion; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure*; D013347:Subcellular Fractions / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D037742:Nanotubes, Carbon