title: Ultrahigh pressure extraction of lignan compounds from Dysosma versipellis and purification by high-speed counter-current chromatography.
abstract: Ultrahigh pressure extraction (UPE) was employed to extract podophyllotoxin and 4'-demethylpodophyllotoxin from Dysosma versipellis. The effects of extraction parameters including extraction solvents, pressure, time and solid/liquid ratio were investigated using a High Hydrostatic Pressure Processor. The optimal condition for UPE of the target compounds was 80% methanol, 200 MPa of pressure, 1 min of extraction time and 1:12 (g/mL) of solid/liquid ratio. Podophyllotoxin and 4'-demethylpodophyllotoxin in the crude extract were purified by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water (10:10:8:12, v/v), and the fractions were analyzed by HPLC, ESI-MS and (1)H NMR. As a result, 73.7 mg podophyllotoxin and 16.5mg 4'-demethylpodophyllotoxin with purities over 96% were obtained from 260 mg crude sample in one-step separation.
authors.affiliation: Qilu Hospital of Shangdong University, Jinan, Shandong 250012, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Meixia
authors.initials: M
authors.lastname: Xu
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Lin
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
mesh_terms: D028141:Berberidaceae / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D011034:Podophyllotoxin / Q000819:agonists / Q000031:analogs & derivatives* / Q000302:isolation & purification; D011312:Pressure
chemical_list: D010936:Plant Extracts; D011034:Podophyllotoxin