title: Crystallization and preliminary X-ray analysis of Chandipura virus glycoprotein G.
abstract: Fusion in members of the Rhabdoviridae virus family is mediated by the G glycoprotein. At low pH, the G glycoprotein catalyzes fusion between viral and endosomal membranes by undergoing a major conformational change from a pre-fusion trimer to a post-fusion trimer. The structure of the G glycoprotein from vesicular stomatitis virus (VSV G), the prototype of Vesiculovirus, has recently been solved in its trimeric pre-fusion and post-fusion conformations; however, little is known about the structural details of the transition. In this work, a soluble form of the ectodomain of Chandipura virus G glycoprotein (CHAV G(th)) was purified using limited proteolysis of purified virus; this soluble ectodomain was also crystallized. This protein shares 41% amino-acid identity with VSV G and thus its structure could provide further clues about the structural transition of rhabdoviral glycoproteins induced by low pH. Crystals of CHAV G(th) obtained at pH 7.5 diffracted X-rays to 3.1 Å resolution. These crystals belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 150.3, b = 228.2, c = 78.8 Å. Preliminary analysis of the data based on the space group and the self-rotation function indicated that there was no trimeric association of the protomers. This unusual oligomeric status could result from the presence of fusion intermediates in the crystal.
authors.affiliation: Centre de Recherche de Gif, Laboratoire de Virologie Moléculaire et Structurale, CNRS (UPR 3296), Avenue de la Terrasse, 91198 Gif-sur-Yvette CEDEX, France.
authors.forename: Eduard
authors.initials: E
authors.lastname: Baquero
authors.forename: Linda
authors.initials: L
authors.lastname: Buonocore
authors.forename: John K
authors.initials: JK
authors.lastname: Rose
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bressanelli
authors.forename: Yves
authors.initials: Y
authors.lastname: Gaudin
authors.forename: Aurélie A
authors.initials: AA
authors.lastname: Albertini
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D006023:Glycoproteins / Q000737:chemistry*; D018116:Vesiculovirus / Q000737:chemistry*; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins