title: Assembly of DNA curtains using hydrogen silsesquioxane as a barrier to lipid diffusion.
abstract: We have established a single-molecule imaging experimental platform called "DNA curtains" in which DNA molecules tethered to a lipid bilayer are organized into patterns at nanofabricated metallic barriers on the surface of a microfluidic sample chamber. This technology has wide applications for real-time single-molecule imaging of protein-nucleic acid interactions. Here, we demonstrate that DNA curtains can also be made from hydrogen silsesquioxane (HSQ). HSQ offers important advantages over metallic barriers because it can be lithographically patterned directly onto fused silica slides without any requirement for further processing steps, thereby offering the potential for rapid prototype development and/or scale up for manufacturing.
authors.affiliation: Department of Applied Physics and Applied Mathematics, Center for Electron Transport in Molecular Nanostructures, Columbia University, New York, New York 10027, United States.
authors.forename: T A
authors.initials: TA
authors.lastname: Fazio
authors.forename: Ja Yil
authors.initials: JY
authors.lastname: Lee
authors.forename: S J
authors.initials: SJ
authors.lastname: Wind
authors.forename: E C
authors.initials: EC
authors.lastname: Greene
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004058:Diffusion; D008051:Lipid Bilayers / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D017646:Organosilicon Compounds / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: C512088:1,3-bis(1,1,3,3,5,5-hexaethoxy-1,3,5-trisilacyclohexyl)propane; D008051:Lipid Bilayers; D008055:Lipids; D017646:Organosilicon Compounds; D012822:Silicon Dioxide; D004247:DNA