title: A strategy for detecting absorbed bioactive compounds for quality control in the water extract of rhubarb by ultra performance liquid chromatography with photodiode array detector.
abstract: OBJECTIVE
  To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment.
  
  
  METHODS
  Anthraquinones in water extract of rhubarb (WER), in cerebrospinal fluid (CSF) of patients with traumatic brain injury (TBI) and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector (UPLC-PDA) method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase (SOD) activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER.
  
  
  RESULTS
  Anthraquinones in WER were aloe-emodin, rhein, emodin, chrysophanol, and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly.
  
  
  CONCLUSIONS
  Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy, the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.
authors.affiliation: Institute of Integrated Traditional Chinese and Western Medicine, and the National Key Clinical Specialist Vocational School of Traditional Chinese Medicine Encephalopathy, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xi
authors.initials: X
authors.lastname: Huang
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Liang
authors.forename: Rong
authors.initials: R
authors.lastname: Fan
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.forename: Kui-Po
authors.initials: KP
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jie-Kun
authors.initials: JK
authors.lastname: Luo
authors.forename: Yun-Hui
authors.initials: YH
authors.lastname: Li
authors.forename: Xi-Lian
authors.initials: XL
authors.lastname: Mao
authors.forename: Zhao-Qian
authors.initials: ZQ
authors.lastname: Liu
authors.forename: Hong-Hao
authors.initials: HH
authors.lastname: Zhou
mesh_terms: D000042:Absorption / Q000187:drug effects; D000818:Animals; D000880:Anthraquinones / Q000134:cerebrospinal fluid / Q000737:chemistry; D001688:Biological Products / Q000032:analysis* / Q000134:cerebrospinal fluid / Q000737:chemistry; D001930:Brain Injuries / Q000188:drug therapy / Q000473:pathology; D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D004642:Emodin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D012250:Rheum / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; D001688:Biological Products; D010936:Plant Extracts; D014867:Water; C008905:physcione; D004642:Emodin