title: The neuroprotective effect of overexpression of calbindin-D(28k) in an animal model of Parkinson's disease.
abstract: Overexpression of calbindin-D(28k) (CaBP-28 k) induces neurite outgrowth in dopaminergic neuronal cells and could provide some protection to dopaminergic neurons against the pathological process in Parkinson's disease. Transgenic mice CaBP-28 k overexpression and the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse models were generated, and the effect of midbrain dopamine neurons in ethology was also assessed. Tyrosine hydroxylase (TH)-immunoreactive neurons were counted, and the concentration of total protein and dopamine (DA) of striatum corpora was measured in four animal models. Results showed that the positive TH cells, content of DA, and ability of ethology in MPTP-induced transgenic mice were significantly higher than that in MPTP-induced wild-type mice. The findings demonstrate that overexpression of CaBP-28 k could provide protection for DA neurons from neurodegeneration. It would provide a potential strategy in the treatment of Parkinson's diseases.
authors.affiliation: Department of Neurobiology, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: Hong-Hua
authors.initials: HH
authors.lastname: Yuan
authors.forename: Ren-Jin
authors.initials: RJ
authors.lastname: Chen
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Zhu
authors.forename: Chang-Ling
authors.initials: CL
authors.lastname: Peng
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Zhu
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000818:Animals; D001522:Behavior, Animal; D015153:Blotting, Western; D064026:Calbindins; D053148:Caspase 3 / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018696:Neuroprotective Agents / Q000378:metabolism*; D010300:Parkinson Disease / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D013378:Substantia Nigra / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D064026:Calbindins; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D064030:S100 Calcium Binding Protein G; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D014446:Tyrosine 3-Monooxygenase; D053148:Caspase 3