title: Microbial biogeography of arctic streams: exploring influences of lithology and habitat.
abstract: Terminal restriction fragment length polymorphism and 16S rRNA gene sequencing were used to explore the community composition of bacterial communities in biofilms on sediments (epipssamon) and rocks (epilithon) in stream reaches that drain watersheds with contrasting lithologies in the Noatak National Preserve, Alaska. Bacterial community composition varied primarily by stream habitat and secondarily by lithology. Positive correlations were detected between bacterial community structure and nutrients, base cations, and dissolved organic carbon. Our results showed significant differences at the stream habitat, between epipssamon and epilithon bacterial communities, which we expected. Our results also showed significant differences at the landscape scale that could be related to different lithologies and associated stream biogeochemistry. These results provide insight into the bacterial community composition of little known and pristine arctic stream ecosystems and illustrate how differences in the lithology, soils, and vegetation community of the terrestrial environment interact to influence stream bacterial taxonomic richness and composition.
authors.affiliation: Rubenstein School of Environment and Natural Resources, University of Vermont Burlington, VT, USA.
authors.forename: Julia R
authors.initials: JR
authors.lastname: Larouche
authors.forename: William B
authors.initials: WB
authors.lastname: Bowden
authors.forename: Rosanna
authors.initials: R
authors.lastname: Giordano
authors.forename: Michael B
authors.initials: MB
authors.lastname: Flinn
authors.forename: Byron C
authors.initials: BC
authors.lastname: Crump