title: Enhanced recognition and neutralization of HIV-1 by antibody-derived CCR5-mimetic peptide variants.
abstract: A tyrosine-sulfated CCR5-mimetic peptide, CCR5mim1, inhibits HIV-1 infection more efficiently than sulfopeptides based on the CCR5 amino terminus. Here we characterized sulfopeptide chimeras of CCR5mim1 and the heavy-chain CDR3 of the antibody PG16. Two chimeras bound a range of envelope glycoproteins and neutralized HIV-1 more efficiently than CCR5mim1. An immunoadhesin form of one of these, CCR5mim2-Ig, synergized with CD4-Ig to neutralize HIV-1. These sulfopeptides are among the broadest and most potent CCR5-mimetic peptides described to date.
authors.affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, New England Primate Research Center, Southborough, Massachusetts, USA.
authors.forename: Jessica J
authors.initials: JJ
authors.lastname: Chiang
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Gardner
authors.forename: Brian D
authors.initials: BD
authors.lastname: Quinlan
authors.forename: Tatyana
authors.initials: T
authors.lastname: Dorfman
authors.forename: Hyeryun
authors.initials: H
authors.lastname: Choe
authors.forename: Michael
authors.initials: M
authors.lastname: Farzan
mesh_terms: D057134:Antibodies, Neutralizing / Q000737:chemistry; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D000939:Epitopes / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D057809:HEK293 Cells; D015658:HIV Infections / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D009500:Neutralization Tests; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D019713:Receptors, CCR5 / Q000737:chemistry / Q000378:metabolism*
chemical_list: D057134:Antibodies, Neutralizing; D000939:Epitopes; D006023:Glycoproteins; D010455:Peptides; D019713:Receptors, CCR5