title: Antiallergic asthma properties of brazilin through inhibition of TH2 responses in T cells and in a murine model of asthma.
abstract: This study aimed to determine whether brazilin exhibits anti-inflammatory effects that inhibit T helper cell type II (T(H)2) responses and whether it suppresses allergic inflammation reactions in a murine model of asthma. We found that brazilin inhibited the mRNA and protein expression of interleukin (IL)-4 and IL-5 induced by phorbol myristate acetate (PMA) and cAMP in EL-4 T cells in a dose-dependent manner. Following the intratracheal instillation of brazilin in ovalbumin (OVA)-immunized mice, we found that brazilin-treated mice exhibited decreases in the release of IL-4, IL-5, IL-13, eotaxin-1, and tumor necrosis factor-α in bronchoalveolar lavage fluid (BALF); inhibited T(H)2 functioning via a decrease in IL-4 production; and exhibited attenuation of OVA-induced lung eosinophilia, airway hyperresponsiveness, and airway remodeling. These results suggest that brazilin exhibits anti-T(H)2 effects both in vitro and in vivo and may possess therapeutic potential for allergic diseases.
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, College of Medicine, China Medical University, Taichung, Taiwan. leechenchen@mail.cmu.edu.tw
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Lee
authors.forename: Chien-Neng
authors.initials: CN
authors.lastname: Wang
authors.forename: Jaw-Jou
authors.initials: JJ
authors.lastname: Kang
authors.forename: Jiunn-Wang
authors.initials: JW
authors.lastname: Liao
authors.forename: Bor-Luen
authors.initials: BL
authors.lastname: Chiang
authors.forename: Hui-Chen
authors.initials: HC
authors.lastname: Chen
authors.forename: Chien-Ming
authors.initials: CM
authors.lastname: Hu
authors.forename: Chia-Der
authors.initials: CD
authors.lastname: Lin
authors.forename: Shih-Hsuan
authors.initials: SH
authors.lastname: Huang
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Lai
mesh_terms: D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001249:Asthma / Q000188:drug therapy* / Q000276:immunology*; D001578:Benzopyrans / Q000494:pharmacology / Q000627:therapeutic use*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D054413:Chemokine CCL11 / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D018793:Interleukin-13 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics; D015848:Interleukin-5 / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D018927:Anti-Asthmatic Agents; D001578:Benzopyrans; D054413:Chemokine CCL11; D018793:Interleukin-13; D015848:Interleukin-5; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D010047:Ovalbumin; C044362:brazilin