title: Bone morphogenetic protein 4 (BMP-4) and BMP-7 induce vascular endothelial growth factor expression in bovine granulosa cells.
abstract: Bone morphogenetic protein-4 (BMP-4) and BMP-7, theca cell-derived growth factors, directly affect the granulosa cell function. The aim of this study was to examine the involvement of BMP-4 or BMP-7 in vascular endothelial growth factor (VEGF) expression in bovine granulosa cells. Granulosa cells were collected from small follicles (4-6 mm) and seeded at a density of 2-5 × 10(5) cells per well in Dulbecco's modified Eagle's medium (DMEM)/F12 medium with BMP-4 or BMP-7. The expression of VEGF messenger RNA and protein was the maximum when 1.0 ng/mL of BMP-4 was added to the culture medium. On the other hand, 10 ng/mL of BMP-7 significantly increased the expression of the VEGF gene and protein. In addition, BMP-4 stimulated the expression of Smad1 and Smad5 genes in granulosa cells, whereas BMP-7 stimulated the expression of Smad5 gene. These results suggested that BMP-4 and BMP-7 may be associated with VEGF expression via several specific Smads in bovine granulosa cells: BMP-4 via Smad1/Smad5 and BMP-7 via Smad5. In conclusion, theca cell-derived BMP-4 and BMP-7 might contribute to follicular vasculature and development by inducing VEGF expression in granulosa cells.
authors.affiliation: Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine Inada-machi, Obihiro, Hokkaido, Japan. shimizut@obihiro.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Fumie
authors.initials: F
authors.lastname: Magata
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Abe
authors.forename: Akio
authors.initials: A
authors.lastname: Miyamoto
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000502:physiology*; D055419:Bone Morphogenetic Protein 7 / Q000502:physiology*; D002417:Cattle / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D015870:Gene Expression; D006107:Granulosa Cells / Q000737:chemistry*; D012313:RNA / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D048348:Reverse Transcription; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000235:genetics
chemical_list: D055415:Bone Morphogenetic Protein 4; D055419:Bone Morphogenetic Protein 7; D042461:Vascular Endothelial Growth Factor A; D012313:RNA