title: Evaluation of the remineralization potential of amorphous calcium phosphate and fluoride containing pit and fissure sealants using scanning electron microscopy.
abstract: OBJECTIVE
  To evaluate the remineralization potential of Amorphous Calcium Phosphate (ACP) and Fluoride containing pit and Fissure Sealants using Scanning Electron Microscopy.
  
  
  METHODS
  Thirty maxillary first premolars were divided into three groups of ten each and were randomly selected for ACP containing (Aegis- Opaque White, Bosworth Co. Ltd.), Fluoride containing (Teethmate F1 Natural Clear, Kuraray Co. Ltd.), resin based (Concise- Opaque White, 3M ESPE Co. Ltd.) pit and fissure sealant applications. The Concise group served as a control. The teeth weresubjected to the pH-cycling regimen for a period of two weeks. After two weeks, the teeth were sectioned bucco-lingually into 4mm sections and were observed under Scanning Electron Microscope at 50X, 250X, 500X, 1000X and 1500X magnifications. The qualitative changes at the tooth surface and sealant interface were examined and presence of white zone at the interface was considered positive for remineralization.
  
  
  RESULTS
  Both ACP containing (Aegis) and Fluoride containing (Teethmate F1) group showed white zone at the tooth surface-sealant interface. The resin based group (Concise) showed regular interface between the sealant and the tooth structure, but no clear cut white zone was observed.
  
  
  CONCLUSIONS
  Both, Aegis and Teethmate F1 have the potential to remineralize. Release of Amorphous Calcium Phosphate molecules in Aegis group and formation of Fluoroapetite in Teethmate F1 group, were probably responsible for the remineralization.
authors.affiliation: Department of Pedodontics and Preventive Dentistry, College of Dental Science and Research Centre, Lucknow, India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Choudhary
authors.forename: Shobha
authors.initials: S
authors.lastname: Tandon
authors.forename: M
authors.initials: M
authors.lastname: Ganesh
authors.forename: Anshul
authors.initials: A
authors.lastname: Mehra
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate / Q000627:therapeutic use; D002130:Calcium Phosphates / Q000627:therapeutic use*; D002327:Cariostatic Agents / Q000627:therapeutic use*; D003738:Dental Cements / Q000627:therapeutic use; D003743:Dental Enamel / Q000187:drug effects / Q000648:ultrastructure; D005459:Fluorides / Q000627:therapeutic use*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010895:Pit and Fissure Sealants / Q000627:therapeutic use*; D012464:Saliva, Artificial / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D014070:Tooth / Q000187:drug effects / Q000648:ultrastructure*; D017001:Tooth Demineralization / Q000503:physiopathology; D014089:Tooth Remineralization / Q000379:methods*
chemical_list: C528777:Aegis Ortho adhesive; D002130:Calcium Phosphates; D002327:Cariostatic Agents; D003738:Dental Cements; D010895:Pit and Fissure Sealants; D012464:Saliva, Artificial; C519480:amorphous calcium phosphate; C099291:teethmate-FTM; D017438:Bisphenol A-Glycidyl Methacrylate; D005459:Fluorides