title: Sweet kiss of dying cell: sialidase activity on apoptotic cell is able to act toward its neighbors.
abstract: Apoptotic cells and subcellular microparticles expose increased sialidase activity on their surfaces, which results from caspase-3 dependent activation of plasma membrane associated Neuraminidase-1 (Neu1). Desialylation of dying cells is also known to promote efferocytosis. The intriguing question remained whether sialidase on the surface of dying cell merely acts on self targets (cis-action), or whether it can also cleave glycoepitopes of neighboring cells (trans-action). Here, we co-incubated human viable and apoptotic Jurkat lymphocytes or neutrophils with human erythrocytes and evaluated their glycoprofile for terminal sialic acids by agglutination assay, flow cytometry, ELISA and dot-blot analyses. Data suggest that erythrocytes were desialylated as soon as 3 hours after co-incubation with apoptotic cells, but not with viable ones. After co-incubation of L929 murine fibroblasts with viable or apoptotic murine L1210 cells the L929 cells gained a desialylated glycoprofile, only after co-incubation with apoptotic cells. Our data suggests that activated sialidase(s) on the surfaces of apoptotic cells are capable to desialylate neighboring cells in trans.
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine.
authors.forename: Tanya
authors.initials: T
authors.lastname: Shkandina
authors.forename: Martin
authors.initials: M
authors.lastname: Herrmann
authors.forename: Rostyslav
authors.initials: R
authors.lastname: Bilyy
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018920:Coculture Techniques; D004789:Enzyme Activation; D004912:Erythrocytes; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D007739:L Cells; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D009439:Neuraminidase / Q000378:metabolism*; D009504:Neutrophils; D012794:Sialic Acids / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D012794:Sialic Acids; D009439:Neuraminidase; D053148:Caspase 3