title: Three-dimensional ordered macroporous (3DOM) composite for electrochemical study on acetylcholinesterase inhibition induced by endogenous neurotoxin.
abstract: In this paper, an electrochemical acetylcholinesterase (AChE) inhibition assay based on three-dimensional ordered macroporous (3DOM) composite was conducted. The 3DOM composite was first fabricated on the glassy carbon electrode by electropolymerization of aniline in the presence of ionic liquid (IL) on a sacrificial silica nanospheres template. After the silica nanospheres were etched, an IL-doped polyaniline (IL-PANI) film with 3DOM morphology was formed. Then, gold nanoparticles (AuNPs) were decorated on the IL-PANI film by electrodeposition. The immobilized AChE on the 3DOM composite displayed favorable affinity to substrate acetylthiocholine chloride (ATCh), and the 3DOM composite showed excellent electrocatalytic effect on thiocholine, the hydrolysis product of ATCh. The presence of IL and AuNPs could improve the sensitivity by accelerating the electron transfer. The designed AChE biosensor was successfully applied to evaluate the AChE inhibition induced by endogenous neurotoxin 1(R),2N-dimethyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline [(R)-NMSal]. The results demonstrate that (R)-NMSal exerts a considerable effect on AChE activity, and the inhibition is reversible. The developed method offers a new approach for AChE inhibition assay, which is of great benefit in understanding the mechanism behind neurotoxin-induced neurodegenerative disorders.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai 200062, PR China.
authors.forename: Yingqiao
authors.initials: Y
authors.lastname: Teng
authors.forename: Ying
authors.initials: Y
authors.lastname: Fu
authors.forename: Lili
authors.initials: L
authors.lastname: Xu
authors.forename: Bin
authors.initials: B
authors.lastname: Lin
authors.forename: Zhongchuan
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhiai
authors.initials: Z
authors.lastname: Xu
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D055664:Electrochemical Techniques / Q000379:methods; D004593:Electrophorus / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D053761:Nanocomposites / Q000737:chemistry*; D016062:Porosity; D044005:Tetrahydroisoquinolines / Q000494:pharmacology*
chemical_list: D000814:Aniline Compounds; D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D044005:Tetrahydroisoquinolines; C416807:polyaniline; C069193:1,2-dimethyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline; D000110:Acetylcholinesterase