title: Influence of Alternanthera brasiliana (L.) Kuntze on Altered Antioxidant Enzyme Profile during Cutaneous Wound Healing in Immunocompromised Rats.
abstract: Alternanthera brasiliana (L.) Kuntze (Amaranthaceae) is a herbaceous plant used against inflammation, cough, and diarrhea in Brazilian popular medicine. In our preliminary study, promising wound healing activity of methanol extract of leaves of A. brasiliana (MEAB) was observed in normal excision and incision wound models. Therefore, the present study was designed to investigate the wound healing activity along with the antioxidant enzyme profile during cutaneous excision immunocompromised wound after topical application of 5% w/w ointment of MEAB in rats. Immunocompromised state was induced by pretreatment with hydrocortisone (HC) at 40 mg/kg body weight (i.m.) in male rats. Following one-week pretreatment with HC, wounds were created. The vehicle, 5% (w/w) ointment of MEAB, or standard drug (Himax) was applied topically twice daily. Healing potential was evaluated by the rate of wound contraction, estimation of enzymatic and nonenzymatic antioxidants like catalase, SOD, GSH, protein, vitamin C, and hydroxyproline content, which was supported by histopathological study on the 8th day following wounding. There was significant increase in the enzymatic and nonenzymatic antioxidant parameters in the extract-reated group as compared to control group. Histopathological study revealed collagen deposition, fibroblast proliferation, angiogenesis, and development of basement membrane in A. brasiliana group. The results of the present investigation revealed significant wound healing activity of MEAB.
authors.affiliation: Department of Veterinary Pharmacology & Toxicology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781022, India.
authors.forename: Chandana Choudhury
authors.initials: CC
authors.lastname: Barua
authors.forename: Shameem
authors.initials: S
authors.lastname: Ara Begum
authors.forename: Archana
authors.initials: A
authors.lastname: Talukdar
authors.forename: Jayanti
authors.initials: J
authors.lastname: Datta Roy
authors.forename: Bhaben
authors.initials: B
authors.lastname: Buragohain
authors.forename: Debesh
authors.initials: D
authors.lastname: Chandra Pathak
authors.forename: Dilip
authors.initials: D
authors.lastname: Kumar Sarma
authors.forename: Rumi
authors.initials: R
authors.lastname: Saikia Bora
authors.forename: Asheesh
authors.initials: A
authors.lastname: Gupta