title: Identification of immunodominant peptides from Gnathostoma binucleatum.
abstract: Gnathostomiasis is now recognized as a zoonosis with a worldwide distribution. In the Americas, it is caused by the third-stage larvae of Gnathostoma binucleatum and in Asia mainly by G. spinigerum. The availability and preparation of specific antigens are among the main obstacles for developing reliable immunodiagnostic tests. In this study, six immunodominant peptides were identified and characterized from G. binucleatum, somatic antigens (AgS: 24, 32, and 40 kDa) and excretory-secretory antigens (AgES: 42, 44, and 56 kDa) by two-dimensional immunoblot analysis. Among those immunodominant peptides, two AgS spots were characterized by mass spectrometric analysis (32 kDa; pI 6.3 and 6.5) and identified as type 1 galectins. In accordance with this finding, a fraction of AgS exhibited affinity to lactose and displayed a 100% specificity and sensitivity for the diagnosis of human gnathostomiasis.
authors.affiliation: Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, México. calsa68@hotmail.com
authors.forename: Samuel
authors.initials: S
authors.lastname: Campista-León
authors.forename: Francisco
authors.initials: F
authors.lastname: Delgado-Vargas
authors.forename: Abraham
authors.initials: A
authors.lastname: Landa
authors.forename: Kaethe
authors.initials: K
authors.lastname: Willms
authors.forename: Hector Samuel
authors.initials: HS
authors.lastname: López-Moreno
authors.forename: Guillermo
authors.initials: G
authors.lastname: Mendoza-Hernández
authors.forename: Julian
authors.initials: J
authors.lastname: Ríos-Sicairos
authors.forename: Angel Noel
authors.initials: AN
authors.lastname: Bojórquez-Contreras
authors.forename: Sylvia Páz
authors.initials: SP
authors.lastname: Díaz-Camacho
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D006039:Gnathostoma / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000276:immunology; D053719:Tandem Mass Spectrometry
chemical_list: D016056:Immunodominant Epitopes; D010455:Peptides