title: Chemical characteristic of an anticoagulant-active sulfated polysaccharide from Enteromorpha clathrata.
abstract: A sulfated polysaccharide FEP from marine green alga Enteromorpha clathrata was extracted with hot water and further purified by ion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that FEP was a high arabinose-containing sulfated polysaccharide with sulfate ester of 31.0%, and its average molecular weight was about 511kDa. The backbone of FEP was mainly composed of (1→4)-linked β-L-arabinopyranose residues with partially sulfate groups at the C-3 position. In vitro anticoagulant assay indicated that FEP effectively prolonged the activated partial thromboplastin time and thrombin time. The investigation demonstrated that FEP was a novel sulfated polysaccharide with different chemical characteristics from other sulfated polysaccharides from marine algae, and could be a potential source of anticoagulant.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Institute of Marine Drug and Food, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Qi
authors.forename: Wenjun
authors.initials: W
authors.lastname: Mao
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Yin
authors.initials: Y
authors.lastname: Chen
authors.forename: Yanli
authors.initials: Y
authors.lastname: Chen
authors.forename: Chunqi
authors.initials: C
authors.lastname: Zhao
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.forename: Mengxia
authors.initials: M
authors.lastname: Yan
authors.forename: Cong
authors.initials: C
authors.lastname: Lin
authors.forename: Jimiao
authors.initials: J
authors.lastname: Shan
mesh_terms: D000925:Anticoagulants / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013055:Spectrophotometry, Infrared; D013431:Sulfates / Q000737:chemistry*; D044445:Ulva / Q000378:metabolism*
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates