title: The urinary antibiotic 5-nitro-8-hydroxyquinoline (Nitroxoline) reduces the formation and induces the dispersal of Pseudomonas aeruginosa biofilms by chelation of iron and zinc.
abstract: Since cations have been reported as essential regulators of biofilm, we investigated the potential of the broad-spectrum antimicrobial and cation-chelator nitroxoline as an antibiofilm agent. Biofilm mass synthesis was reduced by up to 80% at sub-MIC nitroxoline concentrations in Pseudomonas aeruginosa, and structures formed were reticulate rather than compact. In preformed biofilms, viable cell counts were reduced by 4 logs at therapeutic concentrations. Complexation of iron and zinc was demonstrated to underlie nitroxoline's potent antibiofilm activity.
authors.affiliation: Institute of Medical Microbiology, Jena University Hospital, Jena, Germany. astrid.sobke@gmx.de
authors.forename: A
authors.initials: A
authors.lastname: Sobke
authors.forename: M
authors.initials: M
authors.lastname: Klinger
authors.forename: B
authors.initials: B
authors.lastname: Hermann
authors.forename: S
authors.initials: S
authors.lastname: Sachse
authors.forename: S
authors.initials: S
authors.lastname: Nietzsche
authors.forename: O
authors.initials: O
authors.lastname: Makarewicz
authors.forename: P M
authors.initials: PM
authors.lastname: Keller
authors.forename: W
authors.initials: W
authors.lastname: Pfister
authors.forename: E
authors.initials: E
authors.lastname: Straube
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002413:Cations, Divalent; D002614:Chelating Agents / Q000378:metabolism / Q000494:pharmacology*; D002939:Ciprofloxacin / Q000494:pharmacology; D003091:Colistin / Q000494:pharmacology; D007501:Iron / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D009600:Nitroquinolines / Q000378:metabolism / Q000494:pharmacology*; D010933:Plankton / Q000187:drug effects / Q000254:growth & development; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development; D015032:Zinc / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D002413:Cations, Divalent; D002614:Chelating Agents; D009600:Nitroquinolines; D002939:Ciprofloxacin; C005308:nitroxoline; D007501:Iron; D015032:Zinc; D003091:Colistin