title: Molecular characterization of fluoroquinolone-resistant Aeromonas spp. isolated from imported shrimp.
abstract: Sixty-three nalidixic acid-resistant Aeromonas sp. isolates were obtained from imported shrimp. Phylogenetic analysis of gyrB sequences indicated that 18 were A. enteropelogenes, 26 were A. caviae, and 19 were A. sobria. Double missense mutations in the quinolone resistance-determining region (QRDR) of gyrA at codon 83 (Ser→Val/Ile) and codon 92 (Leu→Met) coupled with a point mutation of parC at codon 80 (Ser→Ile/Phe) conferred high levels of quinolone resistance in the isolates. A majority of A. enteropelogenes and A. caviae strains harbored toxin genes, whereas only a few A. sobria strains harbored these genes. The fluoroquinolone-resistant Aeromonas spp. exhibited higher cytotoxicity than fluoroquinolone-sensitive, virulent Aeromonas spp. to rat epithelial cells.
authors.affiliation: University of Oklahoma, Norman, Oklahoma, USA.
authors.forename: Zakiya
authors.initials: Z
authors.lastname: Shakir
authors.forename: Saeed
authors.initials: S
authors.lastname: Khan
authors.forename: Kidon
authors.initials: K
authors.lastname: Sung
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Khare
authors.forename: Ashraf
authors.initials: A
authors.lastname: Khan
authors.forename: Roger
authors.initials: R
authors.lastname: Steele
authors.forename: Mohamed
authors.initials: M
authors.lastname: Nawaz
mesh_terms: D000333:Aeromonas / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D057585:Aeromonas caviae; D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology*; D005516:Food Microbiology; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D033561:Penaeidae / Q000382:microbiology*; D010802:Phylogeny; D017354:Point Mutation; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D027081:DNA Gyrase