title: [Expression of α(5) subunit of extrasynaptic GABA(A) receptor in insular-kindled rats].
abstract: OBJECTIVE
  To explore the expression of α(5) subunit of extrasynaptic GABA(A) receptor in insular electrical kindled rats and analyze its significance.
  
  
  METHODS
  A total of 96 male Sprague-Dawley rats were divided randomly into kindled, sham-operated and control groups (n = 32). And each group was further divided into 2 sub-groups at different time points. Kindled group: insular electrical kindled via chronic electrical stimulation; Sham-operated group: electrode implantation without electrical stimulation;
  
  
  METHODS
  no operation at all. The number of hippocampal neurons was detected by Nissl staining; the mRNA of α(5) subunit of hippocampus by quantitative-polymerase chain reaction (q-PCR); the expression of α(5) subunit of hippocampus by immunohistochemistry.
  
  
  RESULTS
  No significant difference existed in the number of hippocampal neurons between epileptic and normal rats (P > 0.05). The mRNA levels of α(5) subunit of hippocampus in the sham-operated and control groups were much higher than that of the kindled group at Day 1 and 7 post-sacrificing (P < 0.01). And the hippocampal expressions of α(5) subunit were much more in the sham-operated and control groups than that of the kindled group at Day 1 and 7 post-sacrificing (P < 0.01).
  
  
  CONCLUSIONS
  The hippocampal expression of α(5) subunit decreases markedly in insular epilepsy. And α(5) subunit may play an important role in the occurrence and development of insular epilepsy.
authors.affiliation: Ningxia Medical University, Incubation Base of National Key Laboratory for Cerebrocranial Diseases, Yinchuan 750004, China.
authors.forename: En-jie
authors.initials: EJ
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Fa-zheng
authors.initials: FZ
authors.lastname: Shen
authors.forename: Jiang-hua
authors.initials: JH
authors.lastname: Qi
authors.forename: Lin
authors.initials: L
authors.lastname: Miao
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D004827:Epilepsy / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D006624:Hippocampus / Q000378:metabolism*; D007696:Kindling, Neurologic; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000235:genetics / Q000378:metabolism*
chemical_list: C511535:Gabra5 protein, rat; D011963:Receptors, GABA-A