title: Association study of dimethylarginine dimethylaminohydrolase 2 gene             polymorphisms and coronary heart disease.
abstract: While a number of genetic and environmental risk factors for coronary heart             disease (CHD) have been identified, the list of potential risk factors remains             long. One candidate is dimethylarginine dimethylaminohydrolase (DDAH2), which             is known to be polymorphic in humans. The gene product indirectly increases the             endogenous production of nitric oxide, an anti-atherogenic molecule. Therefore,             alterations in DDAH2 activity may indirectly result in an increased risk of CHD.             We studied allele and genotype distributions for two polymorphic loci of DDAH2,             rs805305 and rs2272592, in 180 patients with CHD and 180 healthy controls. Disease             history and other clinical data were recorded. Polymerase chain reaction-restriction             fragment length polymorphism (PCR-RFLP) was used to determine the genotype at             rs805305, and ligase detection reaction (LDR) was used to determine the genotype             at rs2272592. Systolic blood pressure and blood triglyceride and glucose levels             were higher, and history of hypertension, diabetes, smoking and alcohol use was             more common in the patients with CHD (P<0.05). However, the genotype and allele             frequencies at the two polymorphic loci of DDAH2 were not statistically different             between the two groups. Therefore, no association was observed between the DDAH2             polymorphisms at rs805305 and rs2272592 and CHD.
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, PR China. zhengxuag@126.com
authors.forename: Ai-Guo
authors.initials: AG
authors.lastname: Xu
authors.forename: Rong-Mei
authors.initials: RM
authors.lastname: Xu
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Lu
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Li
authors.forename: Qin-Fu
authors.initials: QF
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.forename: Xin
authors.initials: X
authors.lastname: Fu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Meng-Ying
authors.initials: MY
authors.lastname: Yao
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D000581:Amidohydrolases / Q000235:genetics*; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure / Q000502:physiology; D003327:Coronary Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056426:Genetic Loci; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012307:Risk Factors; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D014280:Triglycerides; D000581:Amidohydrolases; C059999:dimethylargininase