title: GFP/HPV-16E6 fusion protein induces apoptosis in MCF-7 and 293T cells             using a transient expression system.
abstract: Since mucosal high-risk human papillomavirus (HPV) E6 can target and degrade             the tumor suppressor p53, it is recognized as a major causative agent of cervical             cancer. However, to date the distribution of high-risk HPV-E6 protein remains             elusive. Thus, in the present study we used a mammalian green fluorescent protein             (GFP) expression system to express a GFP/HPV-16E6 fusion protein (GFP-16E6) in             wild-type (wt) p53 cells, such as MCF-7 and 293T cells to investigate the trafficking             and localization of E6 and p53. Following transfection, we observed that the overexpressed             GFP-16E6 was a nuclear protein, and that endogenous wt p53 localized to the nucleus             together with GFP-16E6. Strikingly, p53 levels were not decreased but increased             in 24 h transfected with pGFP-16E6. Furthermore, we observed significant apoptosis             induced by GFP-16E6, which proved to be dependent on p53 expression.
authors.affiliation: Beijing Haidian Hospital, Beijing 100080, PR China.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Liwei
authors.initials: L
authors.lastname: Yu
authors.forename: Lina
authors.initials: L
authors.lastname: Sun
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics; D057809:HEK293 Cells; D052162:Human papillomavirus 16 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: C068651:E6 protein, Human papillomavirus type 16; D009687:Nuclear Proteins; D009856:Oncogene Proteins, Viral; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D016159:Tumor Suppressor Protein p53; D049452:Green Fluorescent Proteins