title: The search for secreted proteins in prostate cancer by the Escherichia coli ampicillin secretion trap: expression of NBL1 is highly restricted to the prostate and is related to cancer progression.
abstract: OBJECTIVE
  Genes expressed only in cancer tissue or specific organs will be useful molecular markers. To identify genes that encode secreted proteins present in prostate cancer (PCa), we generated Escherichia coli ampicillin secretion trap (CAST) libraries from PCa and normal prostate (NP).
  
  
  RESULTS
  We identified 15 candidate genes that encode secreted proteins present in PCa and NP. Quantitative RT-PCR analysis revealed that MSMB, NBL1 and AZGP1 were expressed with much higher specificity in PCa and NP than in 14 other kinds of normal tissue. We focused on NBL1, which was originally identified as a putative tumor suppressor gene. Western blot analysis revealed that NBL1 protein was highly expressed in both cell lysate and culture media of the DU145 PCa cell line. Immunohistochemical analysis showed that NBL1 expression was highly detected in and restricted to NP and PCa and was significantly down-regulated in PCa. NBL1 expression was significantly reduced according to the tumor stage, Gleason grade and preoperative prostate-specific antigen (PSA) value.
  
  
  CONCLUSIONS
  NBL1 is a secreted protein that is highly restricted to the prostate. Underexpression of NBL1 correlated with PCa progression. NBL1 might be a candidate tumor marker for PCa in addition to PSA.
authors.affiliation: Department of Molecular Pathology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Hayashi
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sentani
authors.forename: Naohide
authors.initials: N
authors.lastname: Oue
authors.forename: Shinya
authors.initials: S
authors.lastname: Ohara
authors.forename: Jun
authors.initials: J
authors.lastname: Teishima
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Anami
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Akio
authors.initials: A
authors.lastname: Matsubara
authors.forename: Wataru
authors.initials: W
authors.lastname: Yasui
mesh_terms: D000368:Aged; D000667:Ampicillin; D000900:Anti-Bacterial Agents; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D018450:Disease Progression; D004926:Escherichia coli; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D009928:Organ Specificity; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; C490087:NBL1 protein, human; D011506:Proteins; D025521:Tumor Suppressor Proteins; D000667:Ampicillin