title: Morphological changes, chitinolytic enzymes and hydrophobin-like proteins as responses of Lecanicillium lecanii during growth with hydrocarbon.
abstract: Lecanicillium lecanii, Verticillium chlamydosporium, V. fungicola var flavidum and Beauveria bassiana were evaluated on their growth with pure n-hexane, toluene and n-hexane:toluene 17:83 (v:v) mixture. Another set of treatments were conducted with colloidal chitin as additional carbon source. All the strains of Lecanicillium were able to grow using hydrocarbons with or without the addition of chitin, although the presence of hydrocarbons showed significant inhibition evidenced by measured biomass, radial growth and microscopic analyses. Degradation of n-hexane ranged within 43 and 62 % and it was higher than that with toluene. The strains L460, L157 and L2149, which presented the highest growth, were further selected for determinations of hydrocarbon consumptions in microcosms. Strain L157 showed the highest consumption of n-hexane (55.6 %) and toluene (52.9 %) as sole carbon source and it also displayed activities of endochitinases, N-acetylhexosaminidase and production of hydrophobins class I and II.
authors.affiliation: Biotechnology Department, Laboratory of Biopolymers, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco, No. 186, 09340 Mexico City, Mexico.
authors.forename: Zaizy
authors.initials: Z
authors.lastname: Rocha-Pino
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Marín-Cervantes
authors.forename: Marlet
authors.initials: M
authors.lastname: Martínez-Archundia
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Soriano-Blancas
authors.forename: Sergio
authors.initials: S
authors.lastname: Revah
authors.forename: Keiko
authors.initials: K
authors.lastname: Shirai
mesh_terms: D000581:Amidohydrolases / Q000096:biosynthesis*; D001203:Ascomycota / Q000254:growth & development*; D002688:Chitinases / Q000096:biosynthesis*; D005656:Fungal Proteins / Q000096:biosynthesis*; D006586:Hexanes / Q000378:metabolism*; D014050:Toluene / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D006586:Hexanes; C026385:n-hexane; D014050:Toluene; D002688:Chitinases; D000581:Amidohydrolases