title: Effect of additives on freeze-drying and storage of Yarrowia lipolytica lipase.
abstract: The extracellular lipase of Yarrowia lipolytica presents numerous potentialities for biotechnological applications. This work describes the development and storage of powders obtained from supernatants containing Y. lipolytica lipase by freeze-drying as downstream process that is important in obtaining a stable lipase powder with high enzymatic activity. Lipase was produced by Y. lipolytica U6 mutant strain in 20-L bioreactor. Non-concentrated cell-free culture supernatant samples were supplemented with different concentrations (0.5-1 %) of maltodextrin and glycerol as additives to freeze-drying. Effects of additives, temperature, pH, and storage time on lipase powders were determined. After addition of additives, freeze-drying yield increased 3.5-fold compared to supernatant without additive. Maltodextrin with 0.5 % concentration gave the best protection of lipase during dehydration treatment and its freeze-drying yield (77 %) is better than other formulations. Lipase powders were stored at 4 and 25 °C for 46 weeks without loss of lipase activity. A common impediment to the production of commercial enzyme is their low-stability aqueous solutions. The present study shows that freeze-dried lipase powders of Y. lipolytica have good stability for storage and various applications.
authors.affiliation: Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, Iran. f.darvishi@ymail.com
authors.forename: Farshad
authors.initials: F
authors.lastname: Darvishi
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Destain
authors.forename: Iraj
authors.initials: I
authors.lastname: Nahvi
authors.forename: Philippe
authors.initials: P
authors.lastname: Thonart
authors.forename: Hamid
authors.initials: H
authors.lastname: Zarkesh-Esfahani
mesh_terms: D019149:Bioreactors; D003470:Culture Media; D004795:Enzyme Stability / Q000187:drug effects*; D005612:Freeze Drying; D005990:Glycerol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology; D013696:Temperature; D025062:Yarrowia / Q000187:drug effects / Q000201:enzymology*
chemical_list: D003470:Culture Media; D011134:Polysaccharides; C008315:maltodextrin; D008049:Lipase; D005990:Glycerol