title: [A novel method for studying nuclear localization signal-mediated nuclear translocation].
abstract: OBJECTIVE
  To establish a new method for studying the mechanism of nuclear localization signal (NLS)-mediated nuclear translocation in living cells.
  
  
  METHODS
  The cells were treated with 67 mg/L 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonate (CHAPS), followed by incubation with 1 g/L wheat germ agglutinin (WGA), and their effects on interferon-  γ (IFN-γ)-induced nuclear translocation of signal transducer and activator of transcription 1 (STAT1) were observed.
  
  
  RESULTS
  Treatment with CHAPS alone had no effect on IFN-γ-induced nuclear translocation of STAT1, while this process was blocked by further WGA incubation.
  
  
  CONCLUSIONS
  We established a new, simple but effective method for studying the mechanism of NLS-mediated nuclear translocation in living cells by perforating the cell membrane with CHAPS treatment.
authors.affiliation: Department of Pathophysiology, Southern Medical University, Guangzhou, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Deng
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Gong
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism*; D002793:Cholic Acids; D003584:Cytological Techniques; D006367:HeLa Cells; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D019913:Nuclear Localization Signals / Q000378:metabolism*; D050794:STAT1 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D002793:Cholic Acids; D019913:Nuclear Localization Signals; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D007371:Interferon-gamma; C028213:3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate