title: Quantification of saponins in Dioscorea panthaica Prain et Burk rhizomes with monolithic column using rapid resolution liquid chromatography coupled with a triple quadruple electrospray tandem mass spectrometry.
abstract: The identification and quantification of saponins in crude extracts are challenging because of their similar structures and weak ultraviolet absorption. In this study, a rapid resolution liquid chromatography coupled with triple quadruple electrospray tandem mass spectrometric method was established to identify and quantify steroid saponins in the rhizomes of Dioscorea panthaica Prain et Burk, a traditional Chinese medicine. Six steroid saponins were simultaneously determined and quantified within 9min. All analytes were subjected to a RP-18e (50mm×2mm) monolithic column using a gradient elution program with a final detection using electrospray ionization tandem mass spectrometry in the negative ionization mode. The rapid resolution liquid chromatography/tandem mass spectrometry method achieved a limit of detection ranging from 0.52 to 20.40ngmL(-1) and a limit of quantification ranging from 1.20 to 75.20ngmL(-1) for the six studied steroidal saponins. The intra-day and inter-day precision (RSD) were less than 5.34%, and the recovery (RSD) was 1.50-3.51%. The highly sensitive and rapid method is suitable for the quantification of steroidal saponins in D. panthaica rhizomes.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China.
authors.forename: Weihao
authors.initials: W
authors.lastname: Wang
authors.forename: Pengyue
authors.initials: P
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.forename: Wenguang
authors.initials: W
authors.lastname: Jing
authors.forename: Liangmian
authors.initials: L
authors.lastname: Chen
authors.forename: An
authors.initials: A
authors.lastname: Liu
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D027742:Dioscorea / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins