title: Influence of oxidative stress on inducing micturition dysfunction following chronic infravesical obstruction and the protective role of an antioxidant diet - association of in vivo and in vitro studies in rats.
abstract: OBJECTIVE
  This study was developed to determine whether the generation of free radicals, induced by ischemia followed by reperfusion in a model of chronic intravesical obstruction in rats, would lead to damage in the detrusor. It also investigates the possible protective action of the flavonoid galangin on the tissue lesion induced by lipid peroxidation.
  
  
  METHODS
  Twenty-one male rats were divided into three groups of seven animals each. Group A was subjected to a sham procedure; group B to partial obstruction of the bladder neck; and group C to partial obstruction of the bladder neck, but also received a diet rich in the flavonoid galangin. All the animals were subjected to urodynamic evaluation and then sacrificed. The bladders were sent for enzymatic tests.
  
  
  RESULTS
  The urodynamic showed that group B developed significantly greater numbers of involuntary contractions of the detrusor, greater post-micturition residue and lower compliance. The group A presented TEAC levels greater than to the group B. Comparative analysis of group A, B and C demonstrated significantly greater malondialdehyde levels in group B in relation to groups A and C. The group B presented smaller contraction amplitudes than did groups A and C, in electrically stimulated contractions.
  
  
  CONCLUSIONS
  That oxidative stress is implicated in the damage to the detrusor musculature following a period of chronic intravesical obstruction. We show, for the first time, that administration of an antioxidant prior to and following the start of chronic obstruction makes it possible to avoid the cellular lesions that cause detrusor dysfunction.
authors.affiliation: Geriatric Urology Service, Federal University of São Paulo (UNIFESP), SP, Brazil.
authors.forename: Sérgio
authors.initials: S
authors.lastname: Bisogni
authors.forename: Fabio Thadeu
authors.initials: FT
authors.lastname: Ferreira
authors.forename: Arnaldo
authors.initials: A
authors.lastname: Amstalden Neto
authors.forename: Leandro Oliveira
authors.initials: LO
authors.lastname: Chiarelli
authors.forename: Valdemar
authors.initials: V
authors.lastname: Ortiz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D055724:Electrophysiological Phenomena; D005419:Flavonoids / Q000008:administration & dosage; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009119:Muscle Contraction; D009153:Mutagens / Q000008:administration & dosage; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D001743:Urinary Bladder / Q000201:enzymology; D001748:Urinary Bladder Neck Obstruction / Q000209:etiology* / Q000503:physiopathology; D014555:Urination Disorders / Q000209:etiology*; D014563:Urodynamics
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D009153:Mutagens; C037032:galangin; D008315:Malondialdehyde