title: In vitro infection with classical swine fever virus inhibits the transcription of immune response genes.
abstract: BACKGROUND
  Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mechanism through which each gene responds to CSFV infection remains unclear.
  
  
  RESULTS
  In this study, the amplification standard curve and corresponding linear regression equations for the genes SLA-2, TAP1, SLA-DR, Ii, CD40, CD80, CD86, IFN-α, and IFN-β were established successfully. Real-time RT-PCR was used to quantify the immune response gene transcription in PK-15 cells post CSFV infection. Results showed that: (1) immune response genes were generally down-regulated as a result of CSFV infection, and (2) the expression of SLA-2, SLA-DR, Ii and CD80 was significantly decreased (p < 0.001).
  
  
  CONCLUSIONS
  We conclude that in vitro infection with CSFV inhibits the transcription of host immune response genes. These findings may facilitate the development of effective strategies for controlling CSF.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, China.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.forename: Xiao-Quan
authors.initials: XQ
authors.lastname: Li
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Xian-Ming
authors.initials: XM
authors.lastname: Meng
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Zhang
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Liang
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Shi-Kai
authors.initials: SK
authors.lastname: Sun
authors.forename: Xin-Bin
authors.initials: XB
authors.lastname: Cai
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Su
authors.forename: Shan
authors.initials: S
authors.lastname: Yin
authors.forename: Yan-Sheng
authors.initials: YS
authors.lastname: Li
authors.forename: Ting Rong
authors.initials: TR
authors.lastname: Luo
mesh_terms: D000818:Animals; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000276:immunology*; D020869:Gene Expression Profiling; D005802:Genes, MHC Class II; D054884:Host-Pathogen Interactions; D057131:Immune Evasion; D007108:Immune Tolerance; D060888:Real-Time Polymerase Chain Reaction; D013552:Swine; D014158:Transcription, Genetic