title: Oxidative stress  proteins as an indicator of a low quality of eucalyptus clones for the pulp and  paper industry.
abstract: Eucalyptus is a genus widely cultivated in many tropical and subtropical regions of  the world as one of the main sources of raw materials for the pulp and paper  industry. Identification of clones and selection of genotypes with desirable  agronomic characteristics would be useful. We assessed eucalyptus full-sibs  that varied in wood quality, using a combination of two-dimensional gel  electrophoresis and mass spectrometry to identify differentially expressed  proteins as candidates for quality markers. Thirty-one differently expressed  proteins were identified, including three proteins of clone X1, four of clone  X2, and 12 each of clones X3 and X4. These proteins are involved in various  biological processes, including polyphosphate biosynthesis, catalytic activity,  nucleotide excision repair, cellular metabolic processes, cell redox  homeostasis, response to salt stress, response to temperature, oxidation and  reduction processes, cellular water homeostasis, and protein phosphorylation.  In the cambial region of each clone, the proteins ketol-acid reductoisomerase,  uncharacterized protein MG428, receptor-like serine/threonine-protein kinase  and a heat shock protein were found in larger quantities in clone X4 than in  clone X1. These proteins are known to be related to protection against  oxidative stress and biosynthesis of lignin. A high buildup of proteins  involved in response to stress in the cambial region of eucalyptus would  indicate clones with undesirable characteristics for use in the pulp and paper  industry.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
authors.forename: D S
authors.initials: DS
authors.lastname: Britto
authors.forename: C P
authors.initials: CP
authors.lastname: Pirovani
authors.forename: E R
authors.initials: ER
authors.lastname: Gonzalez
authors.forename: J F
authors.initials: JF
authors.lastname: Silva
authors.forename: A S
authors.initials: AS
authors.lastname: Gesteira
authors.forename: J C M
authors.initials: JC
authors.lastname: Cascardo
mesh_terms: D002853:Chromatography, Liquid; D002999:Clone Cells; D015180:Electrophoresis, Gel, Two-Dimensional; D005052:Eucalyptus / Q000378:metabolism*; D007221:Industry; D018384:Oxidative Stress; D010209:Paper / Q000592:standards*; D010940:Plant Proteins / Q000145:classification / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D014934:Wood / Q000378:metabolism; D052584:Xylem / Q000378:metabolism
chemical_list: D010940:Plant Proteins