title: [Establishment of optimized neuronal differentiation-promoting model derived from P19 embryonic carcinoma cells].
abstract: To establish an optimized primary drug screen model of neuronal differentiation using P19 embryonal carcinoma cells.
  
  
  
  The final concentration of retinoid acid (RA), days of suspension culture, manner of adherent culture, suitable cell density and adherent culture medium were tested, respectively. Two stages of neuronal differentiation were examined based on morphological changes and immunocytochemistry analysis of neuronal specific protein β-tubulin III.
  
  
  
  On d 8 of differentiation culture, neuron-like cells were observed with final concentration of 1 μmol/L RA. Neuron-like network was formed on d 16 of neuronal differentiation. β-tubulin III was positively stained on both stages, indicating P19 cells were differentiated into neurons.
  
  
  
  The model using RA to induce P19 embryonic carcinoma cells to differentiate into neuron-like cells has been successfully established, which may provide a rapid, phenotypic cell-based platform for primary screening of neurogenesis-promoting drugs.
authors.affiliation: Division of Cardio-Cerebral Vascular and Hepatic Pharmacology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yu-qin
authors.initials: YQ
authors.lastname: Mei
authors.forename: Xing-guang
authors.initials: XG
authors.lastname: Liang
authors.forename: Yi-jia
authors.initials: YJ
authors.lastname: Lou
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Yu
authors.forename: Dan-yan
authors.initials: DY
authors.lastname: Zhu
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D054278:Embryonal Carcinoma Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D055495:Neurogenesis / Q000187:drug effects; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010641:Phenotype; D014212:Tretinoin / Q000494:pharmacology; D014404:Tubulin / Q000378:metabolism
chemical_list: D014404:Tubulin; D014212:Tretinoin