title: Titrated extract of Centella asiatica provides a UVB protective effect             by altering microRNA expression profiles in human dermal fibroblasts.
abstract: The titrated extract of Centella asiatica (TECA) is a reconstituted mixture             comprising of asiatic acid, madecassic acid, asiaticoside and madecassoside, and             is used as a therapeutic agent in wound healing and also as an anti-microbial,             anticancer and anti-aging agent. Although these properties and the associated             cell signaling pathways have been elucidated, the cellular mechanism of anti-photoaging             upon ultraviolet (UV) exposure in normal human dermal fibroblasts (NHDFs) remains             unknown. In this study, we investigated the photoprotective role of TECA via microRNA             (miRNA) expression profiling analysis. Low dose of TECA did not exhibit toxicity             and showed a protective effect against UVB irradiation in NDHFs. miRNA microarray             experiments revealed that specific miRNAs were altered by TECA stimulation in             UVB-irradiated NHDFs. Functional bioinformatic analysis showed that the putative             target genes of the altered miRNAs were associated with the positive regulation             of cell proliferation, anti-apoptosis, small GTPase- and Ras-mediated signal transduction             and activation of MAPKK. Therefore, these results suggest that TECA may serve             as a potential natural chemoprotective agent against UVB-mediated damage in NHDFs             through changes in the expression of specific miRNAs.
authors.affiliation: Korea Institute for Skin and Clinical Sciences, Konkuk University,                     Seoul 143-701, Republic of Korea.
authors.forename: In-Sook
authors.initials: IS
authors.lastname: An
authors.forename: Sungkwan
authors.initials: S
authors.lastname: An
authors.forename: Sang-Mo
authors.initials: SM
authors.lastname: Kang
authors.forename: Tae-Boo
authors.initials: TB
authors.lastname: Choe
authors.forename: Sung Nae
authors.initials: SN
authors.lastname: Lee
authors.forename: Hyun Hee
authors.initials: HH
authors.lastname: Jang
authors.forename: Seunghee
authors.initials: S
authors.lastname: Bae
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D028041:Centella; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D034622:RNA Interference; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects*; D014315:Triterpenes / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: C526146:Centella asiatica extract; D035683:MicroRNAs; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D014315:Triterpenes