title: Evidence that the Arabidopsis Ubiquitin C-terminal Hydrolases 1 and 2 associate with the 26S proteasome and the TREX-2 complex.
abstract: The 26S proteasome interacts with a number of different proteins, while the TREX-2 complex is an important component of the mRNA export machinery. In animals and yeast, members of the Ubiquitin C-terminal Hydrolase 37 (UCH37) family are found to associate with the 26S proteasome, but this has not been demonstrated in plants. The Arabidopsis UCH1 and UCH2 are orthologous to UCH37. Here, we show that UCH1 and UCH2 interact with the 26S proteasome lid subunits. In addition, the two UCHs also interact with TREX-2 components. Our data suggest that Arabidopsis UCHs may serve as a link between the 26S proteasome lid complex and the TREX-2 complex.
authors.affiliation: Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, ON Canada.
authors.forename: Gang
authors.initials: G
authors.lastname: Tian
authors.forename: Qing
authors.initials: Q
authors.lastname: Lu
authors.forename: Susanne E
authors.initials: SE
authors.lastname: Kohalmi
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Rothstein
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Cui
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D043222:Ubiquitin Thiolesterase / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D043222:Ubiquitin Thiolesterase; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease