title: Complement factors are secreted in human follicular fluid by granulosa cells and are possible oocyte maturation factors.
abstract: OBJECTIVE
  In this study, we identify components of the complement system present in human follicular fluid that affect oocyte development and maturation.
  
  
  METHODS
  Using bottom-up liquid chromatography/mass spectrometry/mass spectrometry, we identified complement factors as consistently present in human follicular fluid from 15 different subjects.
  
  
  RESULTS
  According to our gene-chip data, these complement factors are actively produced by granulosa cells.
  
  
  CONCLUSIONS
  By applying the computational Ingenuity Pathway Analysis software and database we have identified complement pathways that play a role in oocyte maturation and follicular development.
authors.affiliation: Departments of Medicine, Endocrinology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Yoo
authors.forename: Tetyana
authors.initials: T
authors.lastname: Bolbot
authors.forename: Anna
authors.initials: A
authors.lastname: Koulova
authors.forename: Rita
authors.initials: R
authors.lastname: Sneeringer
authors.forename: Kathrin
authors.initials: K
authors.lastname: Humm
authors.forename: Yossi
authors.initials: Y
authors.lastname: Dagon
authors.forename: Anny
authors.initials: A
authors.lastname: Usheva
mesh_terms: D000293:Adolescent; D000328:Adult; D003165:Complement System Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D015870:Gene Expression; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D009866:Oogenesis; D006080:Ovarian Follicle / Q000166:cytology / Q000000981:diagnostic imaging / Q000254:growth & development* / Q000378:metabolism; D014463:Ultrasonography; D055815:Young Adult
chemical_list: D003165:Complement System Proteins