title: Prognostic value of human papillomavirus and squamous cell carcinoma antigen in head and neck squamous cell carcinoma.
abstract: To clarify the synergistic influence of human papillomavirus (HPV) status and squamous cell carcinoma antigen (SCCA) mRNA expression on head and neck squamous cell carcinoma (HNSCC) prognosis, HPV DNA presence and SCCA1 and SCCA2 mRNA expression were determined by PCR and quantitative real-time RT-PCR, respectively, in 121 patients with primary HNSCC who were receiving curative treatment. HPV DNA was detected in 28.1% (34/121) of HNSCC cases, and only high-risk types (HPV-16, HPV-33, HPV-35 and HPV-58) were observed. Positive HPV status showed a significantly better prognosis than negative HPV status (P = 0.022). An elevated SCCA2/SCCA1 mRNA ratio was an independent predictor of disease recurrence (P = 0.004). In addition, HPV-negative patients with a high SCCA2/SCCA1 ratio (>0.27) had a significantly lower recurrence-free survival rate than HPV-negative patients with a low SCCA2/SCCA1 ratio (P < 0.011). Our findings revealed that both HPV status and the SCCA2/SCCA1 mRNA ratio are independently associated with prognosis in HNSCC. Patients with both a HPV-negative status and a high SCCA2/SCCA1 ratio might need intensified treatment and rigorous follow up after treatment because of the high risk of recurrence.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University of the Ryukyus, Okinawa, Japan. jordandzy@hotmail.com
authors.forename: Zeyi
authors.initials: Z
authors.lastname: Deng
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hasegawa
authors.forename: Yukashi
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Sen
authors.initials: S
authors.lastname: Matayoshi
authors.forename: Asanori
authors.initials: A
authors.lastname: Kiyuna
authors.forename: Shinya
authors.initials: S
authors.lastname: Agena
authors.forename: Takayuki
authors.initials: T
authors.lastname: Uehara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Maeda
authors.forename: Mikio
authors.initials: M
authors.lastname: Suzuki
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006258:Head and Neck Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000821:virology*; D052162:Human papillomavirus 16 / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015843:Serpins / Q000235:genetics* / Q000378:metabolism; D000077195:Squamous Cell Carcinoma of Head and Neck; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D004279:DNA, Viral; D012333:RNA, Messenger; D015843:Serpins; C051072:squamous cell carcinoma-related antigen