title: Determination of fumonisins B₁ and B₂ in Chinese rice wine by HPLC using AQC precolumn derivatisation.
abstract: BACKGROUND
  Fumonisins are a group of naturally occurring mycotoxins produced by various Fusarium species that commonly infect maize and other cereals, including sorghum and rice. In this study a sensitive and selective method was developed for the determination of fumonisins B₁ and B₂ (FB₁ and FB₂) in Chinese rice wine. The method is based on high-performance liquid chromatography and fluorescence detection following precolumn derivatisation with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC).
  
  
  RESULTS
  FB₁ and FB₂ in Chinese rice wine were extracted and purified using strong anion exchange cartridges and derivatised with AQC at room temperature. The AQC derivatives were stable for 5 days. Optimal fluorescence was obtained at an excitation wavelength of 246 nm and an emission wavelength of 390 nm. Chromatography was performed using a C18 column and gradient elution at 1 mL min⁻¹ with methanol and 0.05 mol L⁻¹ phosphate buffer at pH 4. The limit of detection was 6 µg L⁻¹ for both FB₁ and FB₂. The method was successfully applied to the determination of FB₁ and FB₂ in Chinese rice wine, with recoveries of 87.5-94.5% being obtained.
  
  
  CONCLUSIONS
  The established method was stable and sensitive for the determination of FB₁ and FB₂ in Chinese rice wine.
authors.affiliation: Laboratory of Food safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
authors.forename: Liyan
authors.initials: L
authors.lastname: Ma
authors.forename: Wentao
authors.initials: W
authors.lastname: Xu
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: He
authors.forename: Kunlun
authors.initials: K
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yunbo
authors.initials: Y
authors.lastname: Luo
mesh_terms: D000634:Aminoquinolines / Q000737:chemistry; D053000:Analytic Sample Preparation Methods; D002219:Carbamates / Q000737:chemistry; D002681:China; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000737:chemistry; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D037341:Fumonisins / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D012275:Oryza / Q000737:chemistry*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014920:Wine / Q000032:analysis*
chemical_list: C078178:6-aminoquinolyl-N-hydroxysuccinimidyl carbamate; D000634:Aminoquinolines; D002219:Carbamates; D005456:Fluorescent Dyes; D037341:Fumonisins; C056934:fumonisin B2; C056933:fumonisin B1