title: Detection of functional antigen-specific T cells from urine of non-muscle invasive bladder cancer patients.
abstract: To gain further insights into the role of T lymphocytes in immune responses against bladder tumors, we developed a method that monitors the presence of functional antigen-specific T cells in the urine of non-muscle invasive bladder cancer patients. As relatively few immune cells can usually be recovered from urine, we examined different isolation/amplification protocols and took advantage of patients treated with weekly intravesical instillations of Bacillus Calmette-Guérin, resulting in large amounts of immune cells into urine. Our findings demonstrate that, upon in vitro amplification, antigen-specific T cells can be detected by an interferon γ (IFNγ)-specific ELISPOT assay.
authors.affiliation: Department of Urology; Centre Hospitalier Universitaire Vaudois; Lausanne, Switzerland.
authors.forename: Claire
authors.initials: C
authors.lastname: Pieraerts
authors.forename: Virginie
authors.initials: V
authors.lastname: Martin
authors.forename: Patrice
authors.initials: P
authors.lastname: Jichlinski
authors.forename: Denise
authors.initials: D
authors.lastname: Nardelli-Haefliger
authors.forename: Laurent
authors.initials: L
authors.lastname: Derre