title: [The expression of T cell immune-related gene mRNAs in peripheral blood mononuclear cells from patients with venous thromboembolism].
abstract: OBJECTIVE
  To explore the role of T cell-mediated immunity in the pathogenesis of venous thromboembolism (VTE) by analyzing the differential expression of T cell immune-related gene mRNAs peripheral blood mononuclear cells (PBMCs) between VTE patients and controls with GeneChip Human Genome.
  
  
  METHODS
  Human cDNA microarray analysis was employed in PBMCs from 20 VTE patients and 20 hypertensive controls, and random variant model (RVM) corrected t-test was used for statistical analysis of differential gene expression.
  
  
  RESULTS
  Six mRNA stripes including CD(247), CD(3D), CD(3G), Granzyme A (GzmA), Granzyme B (GzmB) and Zeta-chain-associated protein kinase 70 (ZAP70) were found to be associated with T cell-mediated immunity. Significant down-regulation of these six mRNAs was found in the VTE group compared with the controls (15.3050 ± 0.6346 vs 15.8053 ± 0.5567, 13.7878 ± 0.7731 vs 14.3820 ± 0.4857, 13.3299 ± 0.9104 vs 14.1246 ± 0.6011, 14.8893 ± 0.8675 vs 15.5305 ± 0.4624, 15.9113 ± 0.8123 vs 16.4553 ± 0.5055, 14.3652 ± 0.7717 vs 14.3652 ± 0.7717; all P values < 0.05).
  
  
  CONCLUSIONS
  T cells' function including antigen recognition, signal transduction and cytotoxicity was impaired in VTE patients. T cell-mediated immunity dysfunction probably plays an important role in the pathogenesis of VTE.
authors.affiliation: Department of Cardiology, Shanghai Tongji Hospital, Shanghai, China.
authors.forename: Hao-ming
authors.initials: HM
authors.lastname: Song
authors.forename: Zhu
authors.initials: Z
authors.lastname: Gong
authors.forename: Le-min
authors.initials: LM
authors.lastname: Wang
authors.forename: Xiao-yu
authors.initials: XY
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006801:Humans; D007111:Immunity, Cellular; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D054556:Venous Thromboembolism / Q000097:blood / Q000235:genetics* / Q000276:immunology*
chemical_list: D012333:RNA, Messenger