title: Pseudomonas otitidis as a potential biocatalyst for polyhydroxyalkanoates (PHA) synthesis using synthetic wastewater and acidogenic effluents.
abstract: Polyhydroxyalkanoates (PHA) production using Pseudomonas otitidis, a newly isolated strain from PHA producing bioreactor was investigated using synthetic acids (SA) and acidogenic effluents (AE) from biohydrogen reactor at different organic loading rates (OLRs). P. otitidis showed ability to grow and accumulate PHA, with simultaneous waste remediation. AE showed less PHA production (54%, OLR3), than SA (58%, OLR2). PHA composition showed co-polymer, poly-3(hydroxy butyrate-co-hydroxy valerate), P3(HB-co-HV). Bioprocess evaluation and enzymatic activities showed good correlation with PHA production. Kinetic studies on the growth of bacteria using different models at varying OLR were substantiated with PHA production. High substrate removal was registered at OLR1 (SA, 87%; AE, 82%). AE could be used as an alternative for pure substrates keeping in view of their high cost.
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500 607, India.
authors.forename: M
authors.initials: M
authors.lastname: Venkateswar Reddy
authors.forename: G N
authors.initials: GN
authors.lastname: Nikhil
authors.forename: S
authors.initials: S
authors.lastname: Venkata Mohan
authors.forename: Y V
authors.initials: YV
authors.lastname: Swamy
authors.forename: P N
authors.initials: PN
authors.lastname: Sarma
mesh_terms: D000143:Acids / Q000378:metabolism*; D001483:Base Sequence; D055162:Biocatalysis; D057919:Biological Oxygen Demand Analysis; D005232:Fatty Acids, Volatile / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008954:Models, Biological; D008969:Molecular Sequence Data; D010802:Phylogeny; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA; D013379:Substrate Specificity; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification
chemical_list: D000143:Acids; D005232:Fatty Acids, Volatile; D054813:Polyhydroxyalkanoates; D012336:RNA, Ribosomal, 16S; D062065:Wastewater