title: Effect of the artificial sweetener, acesulfame potassium, a sweet taste receptor agonist, on glucose uptake in small intestinal cell lines.
abstract: BACKGROUND
  Sweet taste receptors may enhance glucose absorption.
  
  
  OBJECTIVE
  This study aimed to explore the cell biology of sweet taste receptors on glucose uptake.
  
  
  OBJECTIVE
  Artificial sweeteners increase glucose uptake via activating sweet taste receptors in the enterocyte to translocate GLUT2 to the apical membrane through the PLC βII pathway.
  
  
  METHODS
  Caco-2, RIE-1, and IEC-6 cells, starved from glucose for 1 h were pre-incubated with 10 mM acesulfame potassium (AceK). Glucose uptake was measured by incubating cells for 1 to 10 min with 0.5-50 mM glucose with or without U-73122, chelerythrine, and cytochalasin B.
  
  
  RESULTS
  In Caco-2 and RIE-1 cells, 10 mM AceK increased glucose uptake by 20-30 % at glucose >25 mM, but not in lesser glucose concentrations (<10 mM), nor at 1 min or 10 min incubations. U-73122 (PLC βII inhibitor) inhibited uptake at glucose >25 mM and for 5 min incubation; chelerythrine and cytochalasin B had similar effects. No effect occurred in IEC-6 cells.                                                 Activation of sweet taste receptors had no effect on glucose uptake in low (<25 mM) glucose concentrations but increased uptake at greater concentrations (>25 mM).
  
  
  CONCLUSIONS
  Role of artificial sweeteners on glucose uptake appears to act in part by effects on the enterocyte itself.
authors.affiliation: Department of Surgery and the Gastroenterology Research Unit, Mayo Clinic (GU 10-01), 200 1st Street SW, Rochester, MN 55905, USA. zheng.ye@mayo.edu
authors.forename: Ye
authors.initials: Y
authors.lastname: Zheng
authors.forename: Michael G
authors.initials: MG
authors.lastname: Sarr
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018938:Caco-2 Cells; D002460:Cell Line; D020895:Enterocytes / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000493:pharmacokinetics*; D051273:Glucose Transporter Type 2 / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects* / Q000502:physiology; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D054799:Phospholipase C beta / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D013549:Sweetening Agents / Q000378:metabolism / Q000493:pharmacokinetics / Q000494:pharmacology*; D013843:Thiazines / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D051273:Glucose Transporter Type 2; C495680:SLC2A2 protein, human; D013549:Sweetening Agents; D013843:Thiazines; D054799:Phospholipase C beta; D005947:Glucose; C006362:acetosulfame