title: Expression and in situ localization of two major PR proteins of grapevine berries during development and after UV-C exposition.
abstract: In grapevine Vitis vinifera L. cv Pinot noir, the Pathogenesis-Related (PR) proteins CHI4D and TL3 are among the most abundant extractable PR proteins of ripe berries and accumulate during berry ripening from véraison until full maturation. Evidence was supplied in favor of the involvement of these two protein families in plant defense mechanisms and plant development. In order to better understand CHI4D and TL3 function in grapevine, we analyzed their temporal and spatial pattern of expression during maturation and after an abiotic stress (UV-C) by in situ hybridization (ISH) and immunohistolocalization. In ripening berries, CHI4D and TL3 genes were mainly expressed in the exocarp and around vascular bundles of the mesocarp. In UV-C exposed berries, CHI4D and TL3 gene expression was strongly induced before véraison. Corresponding proteins localized in the exocarp and, to a lesser extent, around vascular bundles of the mesocarp. The spatial and temporal accumulation of the two PR proteins during berry maturation and after an abiotic stress is discussed in relation to their putative roles in plant defense.
authors.affiliation: Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne - EA 4707, UFR Sciences Exactes et Naturelles, Université de Reims Champagne-Ardenne, BP 1039, Reims, France.
authors.forename: Steven
authors.initials: S
authors.lastname: Colas
authors.forename: Damien
authors.initials: D
authors.lastname: Afoufa-Bastien
authors.forename: Lucile
authors.initials: L
authors.lastname: Jacquens
authors.forename: Christophe
authors.initials: C
authors.lastname: Clément
authors.forename: Fabienne
authors.initials: F
authors.lastname: Baillieul
authors.forename: Florence
authors.initials: F
authors.lastname: Mazeyrat-Gourbeyre
authors.forename: Laurence
authors.initials: L
authors.lastname: Monti-Dedieu
mesh_terms: D005638:Fruit / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017403:In Situ Hybridization; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D027843:Vitis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant