title: Over-expression of a novel JAZ family gene from Glycine soja, increases salt and alkali stress tolerance.
abstract: Salt and alkali stress are two of the main environmental factors limiting crop production. Recent discoveries show that the JAZ family encodes plant-specific genes involved in jasmonate signaling. However, there is only limited information about this gene family in abiotic stress response, and in wild soybean (Glycine soja), which is a species noted for its tolerance to alkali and salinity. Here, we isolated and characterized a novel JAZ family gene, GsJAZ2, from G. soja. Transcript abundance of GsJAZ2 increased following exposure to salt, alkali, cold and drought. Over-expression of GsJAZ2 in Arabidopsis resulted in enhanced plant tolerance to salt and alkali stress. The expression levels of some alkali stress response and stress-inducible marker genes were significantly higher in the GsJAZ2 overexpression lines as compared to wild-type plants. Subcellular localization studies using a GFP fusion protein showed that GsJAZ2 was localized to the nucleus. These results suggest that the newly isolated wild soybean GsJAZ2 is a positive regulator of plant salt and alkali stress tolerance.
authors.affiliation: Plant Bioengineering Laboratory, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.forename: Hua
authors.initials: H
authors.lastname: Cai
authors.forename: Xiao
authors.initials: X
authors.lastname: Luo
authors.forename: Xi
authors.initials: X
authors.lastname: Bai
authors.forename: Michael K
authors.initials: MK
authors.lastname: Deyholos
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000468:Alkalies / Q000378:metabolism*; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D002467:Cell Nucleus / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D015870:Gene Expression; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D055049:Salt Tolerance / Q000235:genetics*; D055051:Salt-Tolerant Plants / Q000235:genetics / Q000502:physiology*; D013025:Glycine max / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: D000468:Alkalies; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid