title: Antifungal compounds from the rhizome and roots of Ferula hermonis.
abstract: The antifungal activity of hexane, dichloromethane, methanol and aqueous extracts from the rhizome and root of Ferula hermonis was assayed in vitro by the agar disk diffusion method against a panel of human opportunistic and pathogenic fungi. Among them, the hexane and dichloromethane extracts showed the highest activity particularly against the dermatophytes Microsporum gypseum and Tricophyton mentagrophytes as well as the yeast Candida lactis-condensi. Activity-guided fractionation of both extracts using an agar overlay bioautographic method led to the isolation of two antifungal compounds which were identified as the daucane aryl esters jaeschkeanadiol p-hydroxybenzoate (ferutinin) and jaeschkeanadiol benzoate (teferidin). Determination of minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values of both compounds evidenced a stronger antifungal activity for ferutinin than for teferidin. Particularly, T. mentagrophytes was the most sensitive strain with MIC and MFC values ranging from 8 to 256 µg/mL.
authors.affiliation: Unitat de Farmacologia i Farmacognòsia, Facultat de Farmàcia, Universitat de Barcelona, Barcelona, Spain.
authors.forename: Abdel-Hadi
authors.initials: AH
authors.lastname: Al-Ja'fari
authors.forename: Roser
authors.initials: R
authors.lastname: Vila
authors.forename: Blanca
authors.initials: B
authors.lastname: Freixa
authors.forename: Joan
authors.initials: J
authors.lastname: Costa
authors.forename: Salvador
authors.initials: S
authors.lastname: Cañigueral
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects; D001565:Benzoates / Q000302:isolation & purification / Q000494:pharmacology; D001643:Bridged Bicyclo Compounds / Q000302:isolation & purification / Q000494:pharmacology; D002175:Candida / Q000187:drug effects; D003508:Cycloheptanes / Q000302:isolation & purification / Q000494:pharmacology; D005309:Ferula / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008864:Microsporum / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology; D014249:Trichophyton / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D001565:Benzoates; D001643:Bridged Bicyclo Compounds; D003508:Cycloheptanes; D010936:Plant Extracts; D010938:Plant Oils; D012717:Sesquiterpenes; C574822:teferidin; C111068:4-oxy-6-(4-oxybezoyloxy)dauc-8,9-en