title: Arsenite transporters expression in rice (Oryza sativa L.) associated with arbuscular mycorrhizal fungi (AMF) colonization under different levels of arsenite stress.
abstract: As a silicon hyperaccumulator, lowland rice takes up higher levels of As than many other plants due to silicic acid and arsenite sharing the same transporters (Lsi1 and Lsi2). Glomus intraradices (AH01) was inoculated to rice under different arsenite concentrations (0, 2 and 8 μM) in order to investigate the interactions between arbuscular mycorrhizal fungus and rice on the accumulation of arsenite. The relative mRNA expressions of Lsi1 and Lsi2 resulted in a down-regulating trend in mycorrhizal plants. Under 2 μM arsenite treatments, Lsi1 and Lsi2 were significantly decreased, by 0.7-fold (P<0.05) and 0.5-fold (P<0.01), respectively, in mycorrhizal plants when compared with non-mycorrhizal plants. This led to the decrease of arsenite uptake per unit of root dry mass. No organic As species were detected in both roots and shoots. The As(III)/As(V) ratios indicated that mycorrhizal plants immobilized most of the arsenite proportion in the roots and prevented its translocation from the roots to the shoots.
authors.affiliation: Croucher Institute for Environmental Sciences, and Department of Biology, Hong Kong Baptist University, Hong Kong Special Administrative Region.
authors.forename: Xunwen
authors.initials: X
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Wai Fung
authors.initials: WF
authors.lastname: Chan
authors.forename: Chuan
authors.initials: C
authors.lastname: Wu
authors.forename: Fuyong
authors.initials: F
authors.lastname: Wu
authors.forename: Shengchun
authors.initials: S
authors.lastname: Wu
authors.forename: Ming Hung
authors.initials: MH
authors.lastname: Wong
mesh_terms: D018053:Arsenites / Q000378:metabolism* / Q000633:toxicity; D015870:Gene Expression / Q000187:drug effects; D038821:Mycorrhizae / Q000502:physiology*; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012989:Soil Pollutants / Q000378:metabolism* / Q000633:toxicity
chemical_list: D018053:Arsenites; D010940:Plant Proteins; D012989:Soil Pollutants; C015001:arsenite