title: Minireview: applications of next-generation sequencing on studies of nuclear receptor regulation and function.
abstract: Next-generation sequencing technologies have expanded the experimental possibilities for studying the genome-wide regulation of transcription by nuclear receptors, their collaborating transcription factors, and coregulators. These technologies allow investigators to obtain abundance and DNA sequence information in a single experiment. In this review, we highlight proven and potential uses of next-generation sequencing in the study of gene regulation by nuclear receptors. We also provide suggestions on how to effectively leverage this technology in a collaborative environment.
authors.affiliation: Department of Biostatistics and Computational Biology, Harvard School of Public Health, Biostatistics and Computational Biology, 450 Brookline Street, Boston, Massachusetts 02215, USA.
authors.forename: Clifford A
authors.initials: CA
authors.lastname: Meyer
authors.forename: Qianzi
authors.initials: Q
authors.lastname: Tang
authors.forename: X Shirley
authors.initials: XS
authors.lastname: Liu
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D059014:High-Throughput Nucleotide Sequencing; D006657:Histones / Q000378:metabolism; D006801:Humans; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D006657:Histones; D018160:Receptors, Cytoplasmic and Nuclear