title: [Determination of loureirin A and loureirin B in dragon's blood by capillary zone electrophoresis].
abstract: A capillary zone electrophoresis method (CZE) for the simultaneous determination of loureirin A and loureirin B was developed based on the optimized conditions of the pH, composition and concentration of the running buffer solution. Loureirin A and loureirin B were separated and determined effectively within 15 min in a running buffer solution of 20 mmol/L Na2B4O7 (pH 9.98 adjusted with NaOH solution) containing 10.0% (v/v) acetonitrile, 5.0% (v/v) ethylene glycol and 1.0% (v/v) butanol, with the applied voltage of 20 kV, capillary temperature of 25 degrees C, detection wavelength of 211 nm, and injection of 5 s at 3447 Pa. The linear ranges for the determination of loureirin A and loureirin B were 1.00-100 mg/L and 0.50-100 mg/L, respectively. The determination of loureirin A and loureirin B in dragon's blood from natural and artificial inoculation was performed by the proposed method. The relative standard deviations for the determination of the two constituents in samples were from 0.6% to 3.8%, and the recoveries ranged between 95.1% and 105.8%. The method is simple, rapid and possesses higher reproducibility and efficiency. It can be used for the determination of loureirin A and loureirin B in dragon's blood.
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Kunmning 650091, China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Yang
authors.forename: Xufang
authors.initials: X
authors.lastname: Hu
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Xinghong
authors.initials: X
authors.lastname: Wang
authors.forename: Qiue
authors.initials: Q
authors.lastname: Cao
mesh_terms: D047188:Chalcones / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D012116:Resins, Plant / Q000032:analysis*
chemical_list: D047188:Chalcones; D010936:Plant Extracts; D012116:Resins, Plant; C560420:loureirin A; C525191:dragon's blood; C505764:loureirin B