title: The in vitro protective effects of curcumin and demethoxycurcumin in Curcuma longa extract on advanced glycation end products-induced mesangial cell apoptosis and oxidative stress.
abstract: Curcuma longa L. (CLL), a traditional herbal medicine, has been widely used for the prevention of diabetic vascular complications in recent years. However, the protective effects of curcuminoids in CLL on the AGEs-induced damage to mesangial cell are not fully understood. In this present study, dihydroethidium, superoxide dismutase kit, malondialdehyde kit, and acridine orange/ethidium bromide staining methods were used to evaluate the activities of curcumin and demethoxycurcumin (10(-11)-10(-9) M) on AGEs-induced oxidative stress and apoptosis, which were associated with the damage to mesangial cell. The results showed that these two compounds could significantly restore advanced glycation end products (AGEs)-induced apoptosis to normal levels (IC50 = 3.874 × 10(-11) M for curcumin and IC50 = 6.085 × 10(-11) M for demethoxycurcumin) and reduce remarkably reactive oxygen species generation in mesangial cell. Furthermore, curcumin and demethoxycurcumin dramatically elevated AGEs-decreased superoxide dismutase activity while significantly reducing AGEs-increased malondialdehyde content in cell culture supernatant. Our results suggest that both curcumin and demethoxycurcumin have a significant protective potential to the prevention of diabetic nephropathy.
authors.affiliation: Department of Pharmacology, Shaanxi University of Chinese Medicine, Xianyang, PR China.
authors.forename: Ji-ping
authors.initials: JP
authors.lastname: Liu
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.forename: Mao-mao
authors.initials: MM
authors.lastname: Zhu
authors.forename: Ru-Shang
authors.initials: RS
authors.lastname: Wang
authors.forename: Ming-hua
authors.initials: MH
authors.lastname: Zhang
authors.forename: Shao-ying
authors.initials: SY
authors.lastname: Hu
authors.forename: Xiao-bin
authors.initials: XB
authors.lastname: Jia
authors.forename: Jin-Jie
authors.initials: JJ
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology*; D036381:Diarylheptanoids; D004353:Drug Evaluation, Preclinical; D004789:Enzyme Activation / Q000187:drug effects; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D008315:Malondialdehyde / Q000378:metabolism; D050527:Mesangial Cells / Q000187:drug effects*; D018384:Oxidative Stress; D010936:Plant Extracts / Q000737:chemistry; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D011933:Reagent Kits, Diagnostic; D027343:Rhizome / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D036381:Diarylheptanoids; D017127:Glycation End Products, Advanced; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D020011:Protective Agents; D017382:Reactive Oxygen Species; D011933:Reagent Kits, Diagnostic; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D003474:Curcumin; C050229:demethoxycurcumin