title: Gold nanoparticle-enabled real-time ligation chain reaction for ultrasensitive detection of DNA.
abstract: A simple and ultrasensitive colorimetric DNA assay based on the detection of the product of a ligation chain reaction (LCR) and the use of gold nanoparticles (AuNPs) as signal generators has been developed. During LCR, the AuNPs were ligated together, resulting in a distinct color change in real time after a sufficient number of thermal cycles. The cumulative nature of the protocol produced a detection limit of 20 aM with a selectivity factor of 10(3).
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore 117543.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Huimin
authors.initials: H
authors.lastname: Deng
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Gao
mesh_terms: D045744:Cell Line, Tumor; D003124:Colorimetry; D004247:DNA / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D020014:K562 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors
chemical_list: D006046:Gold; D004247:DNA