title: Hepatitis C virus infection of human cytotrophoblasts cultured in vitro.
abstract: Hepatitis C virus (HCV) infection in the uterus is a significant path of vertical HCV transmission. Some studies consider vertical HCV transmission in the uterus as the result of maternal blood leakage into infant blood, whereas others theorize that HCV is transmitted by the mother to the infant through cells constituting the placenta barrier. Although trophoblasts play an important role in the placenta barrier, no definitive evidence has been presented to prove that cytotrophoblasts can be infected with HCV. The current study investigated whether or not these can be infected with HCV by conducting an experiment, in which cultured human cytotrophoblasts were infected with HCV in vitro. The results were analyzed using reverse transcription polymerase chain reaction (RT-PCR), ultrastructural characteristic changes under an electron microscope, and immunoelectron microscopy. HCV RNA in the supernatant of the cultured medium of the infected group was intermittently detected during the 16-day incubation period using RT-PCR. Under an electron microscope, the ultrastructures of infected human cytotrophoblasts were markedly different from normal cells, demonstrating lysosomal hyperplasia, rough endoplasmic reticulum, decreased lipid droplets, presence of vacuoles, and the appearance of HCV-like particles. Using immunoelectron microscopy, HCV-like particles conjoined with golden granules were also observed. Based on the data, the current study concludes that HCV infects a human cytotrophoblast cultured in vitro; moreover, its ultrastructure changes dramatically upon infection.
authors.affiliation: Chinese PLA Centre of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an, China. qinghenie@yeah.net
authors.forename: Qing-He
authors.initials: QH
authors.lastname: Nie
authors.forename: Lu-Hua
authors.initials: LH
authors.lastname: Gao
authors.forename: Yong-Qian
authors.initials: YQ
authors.lastname: Cheng
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Huang
authors.forename: Ya-Fei
authors.initials: YF
authors.lastname: Zhang
authors.forename: Xin-Dong
authors.initials: XD
authors.lastname: Luo
authors.forename: Jun-Qing
authors.initials: JQ
authors.lastname: Wang
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wang
mesh_terms: D005260:Female; D016174:Hepacivirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000648:ultrastructure / Q000821:virology*; D056189:Viral Tropism