title: [Differential expressions of miRNAs in patients with nonvalvular atrial fibrillation].
abstract: OBJECTIVE
  To detect the differential expressions of miRNAs in left atrial appendage (LAA) in patients with atrial fibrillation (AF).
  
  
  METHODS
  Left atrial samples were collected from nonvalvular AF patients and healthy controls. The miRNA transcriptome was analyzed by microarray and verified by real-time reverse transcription-polymerase chain reaction. Computational prediction identified the AF-related miRNAs and its target gene. In the meantime, construction of reporter plasmids and reporter assays were performed to test whether miRNA could represses the Luciferase activity of 3' untranslated regions of its target gene.
  
  
  RESULTS
  MiR-155, miR-142-3p, miR-19b, miR-223, miR-146b-5p, miR-486-5p, miR-301b, miR-193b, miR-519b were found to be up-regulated by > 2 folds whereas miR-193a-5p was down-regulated. In particular, the level of miR-155 increased by 5.78 folds in AF patients versus healthy controls (9.42 ± 4.74 vs 1.63 ± 0.65). Furthermore, computational prediction identified CACNA1C encoding Cav1.2 as a direct target of miR-155. In the meantime, the construction of reporter plasmids and reporter assays showed that miR-155 repressed the Luciferase activity of 3' untranslated regions of CACNA1C.
  
  
  CONCLUSIONS
  In LAA sample of nonvalvular AF, there is an expression of AF-related miRNAs including miR-155. And it reveals a potential link between the regulation of Cav1.2 and miR-155 in electric remodeling of AF.
authors.affiliation: Department of Cardiac Surgery, Capital Medical University, Beijing, China. wangjiangang7545@126.com
authors.forename: Jian-gang
authors.initials: JG
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Meng
authors.forename: Jie
authors.initials: J
authors.lastname: Han
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Tian-ge
authors.initials: TG
authors.lastname: Luo
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Xin
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Xi
mesh_terms: D000328:Adult; D000368:Aged; D001281:Atrial Fibrillation / Q000235:genetics* / Q000503:physiopathology; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D006325:Heart Atria / Q000503:physiopathology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics; D008875:Middle Aged; D014158:Transcription, Genetic
chemical_list: D035683:MicroRNAs