title: Digital PCR strategies in the development and analysis of molecular biomarkers for personalized medicine.
abstract: The efficient delivery of personalized medicine is a key goal of healthcare over the next decade. It is likely that PCR strategies will play an important role in the delivery of this goal. Digital PCR has certain advantages over more traditional PCR protocols. In this article we will discuss the current status of digital PCR, highlighting its advantages and focusing on how it can be utilized in biomarker development and analysis, including the use of individualized biomarkers. We will explore recent developments in this field including examples of how digital PCR may integrate with next generation sequencing to deliver truly personalized medicine.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, United Kingdom. Ekd27@cam.ac.uk
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Day
authors.forename: Paul H
authors.initials: PH
authors.lastname: Dear
authors.forename: Frank
authors.initials: F
authors.lastname: McCaughan
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D056915:DNA Copy Number Variations; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D025202:Molecular Diagnostic Techniques; D010597:Pharmacogenetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D057285:Precision Medicine; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
chemical_list: D015415:Biomarkers