title: Bacterial putative metacaspase structure from Geobacter sulfureducens as a template for homology modeling of type II Triticum aestivum metacaspase (TaeMCAII).
abstract: Metacaspases, cysteine proteases belonging to the peptidase C14 family, are suspected of being involved in the programmed cell death of plants, although their sequences and substrate specificity differ from those of animal caspases. At present, the knowledge on the metacaspase reaction mechanism is based only on biochemical data and homology models constructed on caspase templates. Here we propose a novel template for metacaspase modeling and demonstrate important advantages in comparison to the conventionally used caspase templates. We also point out the connection between plant and bacterial metacaspases, underlining the prokaryotic roots of Programmed Cell Death (PCD).
authors.affiliation: Department of Biometrics and Bioinformatics, Faculty of Agriculture and Biology, Warsaw University of Life Sciences - SGGW, Poland. malgorzata.dudkiewicz@gmail.com
authors.forename: Malgorzata Z
authors.initials: MZ
authors.lastname: Dudkiewicz
authors.forename: Ewa
authors.initials: E
authors.lastname: Piszczek
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D053179:Caspase 7 / Q000737:chemistry; D020169:Caspases / Q000737:chemistry*; D020134:Catalytic Domain; D016923:Cell Death; D030562:Databases, Protein; D045302:Geobacter / Q000201:enzymology*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry; D017510:Protein Folding; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein / Q000379:methods*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014908:Triticum / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins; C505520:CASP7 protein, human; D053179:Caspase 7; D020169:Caspases