title: A specific oligodeoxynucleotide promotes the differentiation of osteoblasts via ERK and p38 MAPK pathways.
abstract: A specific oligodeoxynucleotide (ODN), ODN MT01, was found to have positive effects on the proliferation and activation of the osteoblast-like cell line MG 63. In this study, the detailed signaling pathways in which ODN MT01 promoted the differentiation of osteoblasts were systematically examined. ODN MT01 enhanced the expression of osteogenic marker genes, such as osteocalcin and type I collagen. Furthermore, ODN MT01 activated Runx2 phosphorylation via ERK1/2 mitogen-activated protein kinase (MAPK) and p38 MAPK. Consistently, ODN MT01 induced up-regulation of osteocalcin, alkaline phosphatase (ALP) and type I collagen, which was inhibited by pre-treatment with the ERK1/2 inhibitor U0126 and the p38 inhibitor SB203580. These results suggest that the ERK1/2 and p38 MAPK pathways, as well as Runx2 activation, are involved in ODN MT01-induced up-regulation of osteocalcin, type I collagen and the activity of ALP in MG 63 cells.
authors.affiliation: Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Hou
authors.affiliation: Department of Periodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China.
authors.forename: Liru
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Periodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Immunology, Medical College of Norman Bethune, Jilin University, Changchun 130021, China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Molecular Biology, Medical College of Norman Bethune, Jilin University, Changchun 130021, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun 130021, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Sun
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D002070:Butadienes / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D020935:MAP Kinase Signaling System; D009570:Nitriles / Q000494:pharmacology; D009838:Oligodeoxyribonucleotides / Q000378:metabolism / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D015533:Transcriptional Activation / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D002070:Butadienes; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D007093:Imidazoles; D009570:Nitriles; C000600078:ODN MT01; D009838:Oligodeoxyribonucleotides; D011725:Pyridines; C493709:RUNX2 protein, human; C113580:U 0126; D015675:Osteocalcin; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D000469:Alkaline Phosphatase; C093642:SB 203580