title: Transplantation of adipose-derived stem cells is associated with neural differentiation and functional improvement in a rat model of intracerebral hemorrhage.
abstract: OBJECTIVE
  To examine whether transplantation of adipose-derived stem cells (ADSCs) induces neural differentiation and improves neural function in a rat intracerebral hemorrhage (ICH) model.
  
  
  METHODS
  Adipose-derived stem cells cells were isolated from inguinal fat pad of rat. ICH was induced by injection of collagenase type IV into the right basal ganglia of rat. Forty-eight hours after ICH, ADSCs cells (10 μL of 2-4 × 10(7) cells/mL) were injected into the right lateral cerebral ventricle. The differentiation of ADSCs was detected in vitro and in vivo. The neural function was evaluated with Zea Longa 5-grade scale at day 1, 3, 7, 14, or 28.
  
  
  RESULTS
  Our data demonstrated that ADSCs differentiated into cells that shared the similarities of neurons or astrocytes in vitro. Transplantation of ADSCs decreased cell apoptosis and the transplanted ADSCs were able to differentiate into neuron-like and astrocyte-like cells around the hematoma, accompanied with upregulation of vascular endothelial growth factor expression and improvement of neural function.
  
  
  CONCLUSIONS
  Our data suggest that transplantation of ADSCs could be a therapeutic approach for ICH stroke.
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Department of Neurology, University Hospital of Hubei Institute for Nationalities, Enshi, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Ying-Xin
authors.initials: YX
authors.lastname: Tang
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Liu
authors.forename: Ji
authors.initials: J
authors.lastname: Chen
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Hu
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Shu-Xin
authors.initials: SX
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wen-Gao
authors.initials: WG
authors.lastname: Zeng
authors.forename: Hou-Jie
authors.initials: HJ
authors.lastname: Ni
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Chen
authors.forename: Zhou-Ping
authors.initials: ZP
authors.lastname: Tang
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000704:Analysis of Variance; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001973:Bromodeoxyuridine; D002454:Cell Differentiation / Q000502:physiology*; D002543:Cerebral Hemorrhage / Q000503:physiopathology / Q000601:surgery*; D004195:Disease Models, Animal; D008297:Male; D009474:Neurons / Q000502:physiology*; D010011:Osteocytes / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000502:physiology; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology
chemical_list: D042461:Vascular Endothelial Growth Factor A; D001973:Bromodeoxyuridine