title: Triple subcellular targeting of isopentenyl diphosphate isomerases encoded by a single gene.
abstract: Isopentenyl diphosphate isomerase (IDI) is a key enzyme of the isoprenoid pathway, catalyzing the interconversion of isopentenyl diphosphate and dimethylallyl diphosphate, the universal precursors of all isoprenoids. In plants, several subcellular compartments, including cytosol/ER, peroxisomes, mitochondria and plastids, are involved in isoprenoid biosynthesis. Here, we report on the unique triple targeting of two Catharanthus roseus IDI isoforms encoded by a single gene (CrIDI1). The triple localization of CrIDI1 in mitochondria, plastids and peroxisomes is explained by alternative transcription initiation of CrIDI1, by the specificity of a bifunctional N-terminal mitochondria/plastid transit peptide and by the presence of a C-terminal peroxisomal targeting signal. Moreover, bimolecular fluorescence complementation assays revealed self-interactions suggesting that the IDI likely acts as a multimer in vivo.
authors.affiliation: EA2106 Biomolécules et Biotechnologies Végétales, Université François Rabelais de Tours, Tours, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Guirimand
authors.forename: Anthony
authors.initials: A
authors.lastname: Guihur
authors.forename: Michael A
authors.initials: MA
authors.lastname: Phillips
authors.forename: Audrey
authors.initials: A
authors.lastname: Oudin
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Glévarec
authors.forename: Samira
authors.initials: S
authors.lastname: Mahroug
authors.forename: Céline
authors.initials: C
authors.lastname: Melin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
authors.forename: Marc
authors.initials: M
authors.lastname: Clastre
authors.forename: Nathalie
authors.initials: N
authors.lastname: Giglioli-Guivarc'h
authors.forename: Benoit
authors.initials: B
authors.lastname: St-Pierre
authors.forename: Manuel
authors.initials: M
authors.lastname: Rodríguez-Concepción
authors.forename: Vincent
authors.initials: V
authors.lastname: Burlat
authors.forename: Vincent
authors.initials: V
authors.lastname: Courdavault
mesh_terms: D019748:Carbon-Carbon Double Bond Isomerases / Q000378:metabolism*; D030008:Catharanthus / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D045782:Hemiterpenes / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D009943:Organophosphorus Compounds / Q000378:metabolism; D020675:Peroxisomes / Q000201:enzymology; D010940:Plant Proteins / Q000378:metabolism*; D018087:Plastids / Q000201:enzymology
chemical_list: D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D010940:Plant Proteins; C004809:isopentenyl pyrophosphate; D019748:Carbon-Carbon Double Bond Isomerases