title: Prognostic relevance of melanoma antigen D1 expression in colorectal carcinoma.
abstract: BACKGROUND
  Melanoma antigen D1 (MAGED1) is a member of the type II melanoma antigen (MAGE) family. The down-regulation of MAGED1 expression has been shown in breast carcinoma cell lines and in glioma stem cells and may play an important role in apoptosis and anti-tumorigenesis. However, there is no report on its clinical role in colorectal cancer (CRC).
  
  
  METHODS
  We examined the expression of MAGED1 by qPCR in colorectal cancer tissues and their adjacent non-tumorous tissues taken from 6 cases and performed Western blotting and IHC analyses. In addition, we analyzed MAGED1 expression in 285 clinicopathologically characterized colorectal cancer patients.
  
  
  RESULTS
  MAGED1 expression was significantly down-regulated in colorectal cancer tissues compared with adjacent non-tumorous tissues and was associated with clinical stage (p < 0.001), T classification (p = 0.001), N classification (p < 0.001), M classification (p < 0.001) and pathologic differentiation (p = 0.002). Patients with lower MAGED1 expression had a shorter survival time than those with higher MAGED1 expression. Univariate and multivariate analyses indicated that MAGED1 expression was an independent prognostic factors (p < 0.001).
  
  
  CONCLUSIONS
  MAGED1 may serve as a novel prognostic biomarker of human colorectal cancer.
authors.affiliation: State Key Laboratory of Oncology in Southern China, Guangzhou 510060, China.
authors.forename: Zhao-lei
authors.initials: ZL
authors.lastname: Zeng
authors.forename: Wen-jing
authors.initials: WJ
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Zhen-jie
authors.initials: ZJ
authors.lastname: Tang
authors.forename: Dong-liang
authors.initials: DL
authors.lastname: Chen
authors.forename: Miao-zhen
authors.initials: MZ
authors.lastname: Qiu
authors.forename: Hui-yan
authors.initials: HY
authors.lastname: Luo
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Jin
authors.forename: De-shen
authors.initials: DS
authors.lastname: Wang
authors.forename: Rui-hua
authors.initials: RH
authors.lastname: Xu
mesh_terms: D000951:Antigens, Neoplasm / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000276:immunology*; D015153:Blotting, Western; D015179:Colorectal Neoplasms / Q000276:immunology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008545:Melanoma / Q000276:immunology*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C120897:MAGED1 protein, human; D009363:Neoplasm Proteins