title: Analysis of renal stones by capillary isotachophoresis.
abstract: An analytical method for the determination of the composition of renal stones by capillary isotachophoresis with conductometric detection was developed. Using different leading/terminating electrolyte systems, the qualitative and quantitative analysis of organic compounds (urate, xanthate, oxalate) and inorganic ions (phosphate, Ca(2+), Mg(2+), Na(+), NH(4)(+)) species commonly present in mixed renal stones in three separate steps can be carried out with limits of detection about 10 μmol/L. The developed method was validated by the analysis of real samples and can be used for urinary calculi classification. In addition, it was verified that this method can also be employed for the determination of the above mentioned analytes in some other samples (bones, teeth) concerning apatite biominerals (fluoro-, carbonate-, chloro-apatite).
authors.affiliation: Department of Chemistry, Faculty of Science, Masaryk University, Kotlářská 2, CZ-611 37 Brno, Czech Republic.
authors.forename: Zdeňka
authors.initials: Z
authors.lastname: Jarolímová
authors.forename: Přemysl
authors.initials: P
authors.lastname: Lubal
authors.forename: Viktor
authors.initials: V
authors.lastname: Kanický
mesh_terms: D000838:Anions / Q000652:urine; D001031:Apatites / Q000737:chemistry; D001842:Bone and Bones / Q000737:chemistry; D002254:Carbonates / Q000652:urine; D002413:Cations, Divalent / Q000652:urine; D002414:Cations, Monovalent / Q000652:urine; D003217:Conductometry; D006801:Humans; D057625:Isotachophoresis / Q000379:methods*; D007669:Kidney Calculi / Q000737:chemistry*; D057230:Limit of Detection; D010070:Oxalates / Q000652:urine; D010710:Phosphates / Q000652:urine; D012492:Salts / Q000652:urine; D014070:Tooth / Q000737:chemistry; D014527:Uric Acid / Q000652:urine
chemical_list: D000838:Anions; D001031:Apatites; D002254:Carbonates; D002413:Cations, Divalent; D002414:Cations, Monovalent; D010070:Oxalates; D010710:Phosphates; D012492:Salts; D014527:Uric Acid