title: Dextran and gelatin based photocrosslinkable tissue adhesive.
abstract: A two-component tissue adhesive based on biocompatible and bio-degradable polymers (oxidized urethane dextran (Dex-U-AD) and gelatin) was prepared and photocrosslinked under the ultraviolet (UV) irradiation. The adhesive could adhere to surface of gelatin, which simulated the human tissue steadily. The structures of above Dex-U-AD were characterized by FTIR, (1)H NMR spectroscopy and XRD. The adhesion property of result products was evaluated by lap-shear test. The maximum adhesion strength could reach to 4.16±0.72 MPa which was significantly higher than that of fibrin glue. The photopolymerization process of Dex-U-AD/gelatin was monitored by real time infrared spectroscopy (RTIR). It took less than 5 min to complete the curing process. The cytotoxicity of Dex-U-AD/gelatin also was evaluated which indicated that Dex-U-AD/gelatin gels were nontoxic to L929 cell. The relationship between all the above-mentioned properties and degree of oxidization of Dex-U-AD was assessed. The obtained products have the potential to serve as tissue adhesive in the future.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Nie
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Yang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D005354:Fibrosarcoma / Q000188:drug therapy; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D014014:Tissue Adhesives; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D003911:Dextrans; D020100:Hydrogels; D011108:Polymers; D014014:Tissue Adhesives; D005780:Gelatin