title: Increased mitochondrial activity in BMP7-treated brown adipocytes, due to increased CPT1- and CD36-mediated fatty acid uptake.
abstract: OBJECTIVE
  Brown adipose tissue dissipates chemical energy in the form of heat and regulates triglyceride and glucose metabolism in the body. Factors that regulate fatty acid uptake and oxidation in brown adipocytes have not yet been fully elucidated. Bone morphogenetic protein 7 (BMP7) is a growth factor capable of inducing brown fat mitochondrial biogenesis during differentiation from adipocyte progenitors. Administration of BMP7 to mice also results in increased energy expenditure. To determine if BMP7 is able to affect the mitochondrial activity of mature brown adipocytes, independent of the differentiation process, we delivered BMP7 to mature brown adipocytes and measured mitochondrial activity.
  
  
  RESULTS
  We found that BMP7 increased mitochondrial activity, including fatty acid oxidation and citrate synthase activity, without increasing the mitochondrial number. This was accompanied by an increase in fatty acid uptake and increased protein expression of CPT1 and CD36, which import fatty acids into the mitochondria and the cell, respectively. Importantly, inhibition of either CPT1 or CD36 resulted in a blunting of the mitochondrial activity of BMP7-treated cells.
  
  
  METHODS
  These findings uncover a novel pathway regulating mitochondrial activities in mature brown adipocytes by BMP7-mediated fatty acid uptake and oxidation.
  
  
  CONCLUSIONS
  In conclusion, BMP7 increases mitochondrial activity in mature brown adipocytes via increased fatty acid uptake and oxidation, a process that requires the fatty acid transporters CPT1 and CD36.
authors.affiliation: Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, USA.
authors.forename: Kristy L
authors.initials: KL
authors.lastname: Townsend
authors.forename: Ding
authors.initials: D
authors.lastname: An
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Lynes
authors.forename: Tian Lian
authors.initials: TL
authors.lastname: Huang
authors.forename: Hongbin
authors.initials: H
authors.lastname: Zhang
authors.forename: Laurie J
authors.initials: LJ
authors.lastname: Goodyear
authors.forename: Yu-Hua
authors.initials: YH
authors.lastname: Tseng
mesh_terms: D051698:Activating Transcription Factor 2 / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D052437:Adipocytes, Brown / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D001831:Body Temperature; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism*; D018955:CD36 Antigens / Q000378:metabolism*; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism*; D002384:Catalysis; D019069:Cell Respiration; D004734:Energy Metabolism; D005227:Fatty Acids / Q000378:metabolism*; D015870:Gene Expression; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D008954:Models, Biological; D010084:Oxidation-Reduction; D019289:Pyruvic Acid / Q000378:metabolism; D051896:Smad Proteins, Receptor-Regulated / Q000378:metabolism; D014162:Transfection; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D051698:Activating Transcription Factor 2; D055419:Bone Morphogenetic Protein 7; D018955:CD36 Antigens; D005227:Fatty Acids; D051896:Smad Proteins, Receptor-Regulated; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D002334:Carnitine O-Palmitoyltransferase; D048051:p38 Mitogen-Activated Protein Kinases