title: The effect of rotative stress on CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts.
abstract: This study was designed to explore the effects of rotative stress on carbonic anhydrase II (CAII), TNF receptor superfamily member 6 (FAS), FAS ligand (FASL), osteoclast-associated receptor (OSCAR), and tartrate-resistant acid phosphatase (TRAP) gene expression in osteoclasts. Osteoclasts were induced from RAW264.7 cells cultured in medium containing recombinant murine soluble receptor activator of NF-Kβ ligand (sRANKL). The mRNA and protein expression of CAII, FAS, FASL, OSCAR, and TRAP genes in osteoclasts was detected by RT-PCR and Western blot, respectively, after osteoclasts were loaded at various rotative stress strengths and times. No significant differences in mRNA and protein expression were observed between any of the control groups (P > 0.05). Importantly, rotative stress had a significant effect on the mRNA and protein expression of these genes (P < 0.05). We found a negative relationship between rotative stress strength and prolonged loading time and the expression of FAS/FASL genes in osteoclasts. In addition, there was a positive relationship between rotative stress strength and prolonged loading time and the expression of CAII, OSCAR, or TRAP genes in osteoclasts. Based on these results, rotative stress has a significant effect on CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts.
authors.affiliation: Department of Prosthodontics, Stomatology Hospital, College of Medical Sciences, Zhejiang University, No.395 Yan-an Road, Hangzhou 310006, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Qing Hong
authors.forename: Liu
authors.initials: L
authors.lastname: Meng Tao
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yi
authors.forename: Liu
authors.initials: L
authors.lastname: Wei
authors.forename: Shen
authors.initials: S
authors.lastname: Ju Xiang
authors.forename: Liu
authors.initials: L
authors.lastname: Li
mesh_terms: D000135:Acid Phosphatase / Q000096:biosynthesis; D000818:Animals; D024402:Carbonic Anhydrase II / Q000096:biosynthesis; D002454:Cell Differentiation / Q000502:physiology*; D002478:Cells, Cultured; D053222:Fas Ligand Protein / Q000096:biosynthesis; D015870:Gene Expression; D007527:Isoenzymes / Q000096:biosynthesis; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000378:metabolism; D053245:RANK Ligand / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D012399:Rotation; D013312:Stress, Physiological; D000071681:Tartrate-Resistant Acid Phosphatase; D019014:fas Receptor / Q000096:biosynthesis
chemical_list: D053222:Fas Ligand Protein; C491899:Fas protein, mouse; C505658:Fasl protein, mouse; D007527:Isoenzymes; C450519:Oscar protein, mouse; D053245:RANK Ligand; D011956:Receptors, Cell Surface; C505689:Tnfsf11 protein, mouse; D019014:fas Receptor; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase; D024402:Carbonic Anhydrase II