title: How I treat amyloidosis: the importance of accurate diagnosis and amyloid typing.
abstract: Amyloidosis is a rare group of diseases characterized by deposition of amyloid fibrils in soft tissues. More than 28 types of amyloid have been identified. They all share common ultrastructural and chemical characteristics. Treatments are available for many types but are type specific. Therefore, confirmation and typing of amyloid are essential before initiating treatment. Monoclonal protein studies should be performed on suspected cases, but the diagnosis requires a tissue biopsy. Congo red stain and electron microscopy are helpful to discriminate between amyloid and other pathologic fibrils. Once amyloid is confirmed, typing should be performed. Immunofluorescence and immunohistochemistry are frequently used and are helpful, but this approach has limitations, such as availability, specificity and sensitivity of commercial antibodies. Genetic mutational analysis is vital for ruling in and out hereditary amyloidoses but is unhelpful in nonmutated forms. The most advanced technique of amyloid typing is laser microdissection followed by mass spectrometry. Using proteomics, laser microdissection followed by mass spectrometry can directly identify proteins with or without mutations. Finally, imaging studies, such as cardiac MRI with gadolinium and (123)I-labeled SAP scintigraphy not only assist in evaluation of patients with known amyloidosis but cardiac MRI has detected amyloid in patients previously unsuspected of the disease.
authors.affiliation: Division of Nephrology and Hypertension, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA. leung.nelson@mayo.edu
authors.forename: Nelson
authors.initials: N
authors.lastname: Leung
authors.forename: Samih H
authors.initials: SH
authors.lastname: Nasr
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Sethi
mesh_terms: D000682:Amyloid / Q000032:analysis* / Q000378:metabolism; D000686:Amyloidosis / Q000145:classification / Q000175:diagnosis* / Q000628:therapy; D003224:Congo Red; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D040901:Proteomics
chemical_list: D000682:Amyloid; D003224:Congo Red