title: Development of a unique mouse model for pancreatic cancer lymphatic             metastasis.
abstract: Lymphatic metastasis of pancreatic cancer is a predictor of poor prognosis.             However, the molecular mechanisms are largely unknown, thus, making the development             of appropriate cell lines and experimental models critically important for future             investigations. The purpose of the present study was to establish a 'pancreatic             cancer cell and mouse model with high lymphatic metastasis potential' for in-depth             study of the underlying mechanisms. The BxPC-3-LN subline, derived from the BxPC-3             human pancreatic cancer cell line, was established through serial passages in             nude mice via footpad injections. The subline was able to develop notable lymphatic             metastases in 100% of the recipient mice 8 weeks after tumor cell implantation.             Compared with the parental BxPC-3 cells, BxPC-3-LN cells were more aggressive,             displaying invasive ultrastructure, increased migration and invasion ability,             and chemoresistance. Metastasis-related gene alteration including upregulation             of MMP14, MMP24, MIF and ADRM1, and downregulation of TGFB2 and ROBO1 were also             observed in BxPC-3-LN cells by cDNA microarrays. Thus, the newly selected BxPC-3-LN             subline can serve as a unique model for further study of lymphatic metastasis             of pancreatic cancer.
authors.affiliation: Department of Pancreatic and Hepatobiliary Surgery, Fudan University Shanghai Cancer Center, Shanghai 200032, PR China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Long
authors.forename: Guopei
authors.initials: G
authors.lastname: Luo
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Cui
authors.forename: Kei
authors.initials: K
authors.lastname: Satoh
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Xiao
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Quanxing
authors.initials: Q
authors.lastname: Ni
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Xianjun
authors.initials: X
authors.lastname: Yu
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation; D016000:Cluster Analysis; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D051379:Mice; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology*; D014183:Transplantation, Heterologous; D000093542:Gemcitabine
chemical_list: D000964:Antimetabolites, Antineoplastic; D003841:Deoxycytidine; D000093542:Gemcitabine