title: Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae.
abstract: Macromolecular assemblies involving membrane proteins (MPs) serve vital biological roles and are prime drug targets in a variety of diseases. Large-scale affinity purification studies of soluble-protein complexes have been accomplished for diverse model organisms, but no global characterization of MP-complex membership has been described so far. Here we report a complete survey of 1,590 putative integral, peripheral and lipid-anchored MPs from Saccharomyces cerevisiae, which were affinity purified in the presence of non-denaturing detergents. The identities of the co-purifying proteins were determined by tandem mass spectrometry and subsequently used to derive a high-confidence physical interaction map encompassing 1,726 membrane protein-protein interactions and 501 putative heteromeric complexes associated with the various cellular membrane systems. Our analysis reveals unexpected physical associations underlying the membrane biology of eukaryotes and delineates the global topological landscape of the membrane interactome.
authors.affiliation: Banting and Best Department of Medical Research, Donnelly Centre, 160 College Street, University of Toronto, Toronto, Ontario M5S 3E1, Canada.
authors.forename: Mohan
authors.initials: M
authors.lastname: Babu
authors.forename: James
authors.initials: J
authors.lastname: Vlasblom
authors.forename: Shuye
authors.initials: S
authors.lastname: Pu
authors.forename: Xinghua
authors.initials: X
authors.lastname: Guo
authors.forename: Chris
authors.initials: C
authors.lastname: Graham
authors.forename: Björn D M
authors.initials: BD
authors.lastname: Bean
authors.forename: Helen E
authors.initials: HE
authors.lastname: Burston
authors.forename: Franco J
authors.initials: FJ
authors.lastname: Vizeacoumar
authors.forename: Jamie
authors.initials: J
authors.lastname: Snider
authors.forename: Sadhna
authors.initials: S
authors.lastname: Phanse
authors.forename: Vincent
authors.initials: V
authors.lastname: Fong
authors.forename: Yuen Yi C
authors.initials: YY
authors.lastname: Tam
authors.forename: Michael
authors.initials: M
authors.lastname: Davey
authors.forename: Olha
authors.initials: O
authors.lastname: Hnatshak
authors.forename: Navgeet
authors.initials: N
authors.lastname: Bajaj
authors.forename: Shamanta
authors.initials: S
authors.lastname: Chandran
authors.forename: Thanuja
authors.initials: T
authors.lastname: Punna
authors.forename: Constantine
authors.initials: C
authors.lastname: Christopolous
authors.forename: Victoria
authors.initials: V
authors.lastname: Wong
authors.forename: Analyn
authors.initials: A
authors.lastname: Yu
authors.forename: Gouqing
authors.initials: G
authors.lastname: Zhong
authors.forename: Joyce
authors.initials: J
authors.lastname: Li
authors.forename: Igor
authors.initials: I
authors.lastname: Stagljar
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Conibear
authors.forename: Shoshana J
authors.initials: SJ
authors.lastname: Wodak
authors.forename: Andrew
authors.initials: A
authors.lastname: Emili
authors.forename: Jack F
authors.initials: JF
authors.lastname: Greenblatt
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002687:Chitin Synthase / Q000378:metabolism; D003902:Detergents; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003902:Detergents; D008565:Membrane Proteins; D020543:Proteome; C071940:SSP120 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C555105:Sna2 protein, S cerevisiae; C069390:CHS3 protein, S cerevisiae; D002687:Chitin Synthase