title: Small heat shock protein AgsA: an effective stabilizer of enzyme activities.
abstract: A small heat shock protein, AgsA, possesses chaperone activity that can reduce the amount of heat-aggregated protein in vivo, and suppress the aggregation of chemical- and heat-denatured proteins in vitro. Therefore, we examined the ability of AgsA to stabilize the activity of several enzymes by using this chaperone activity. We observed that AgsA can stabilize the enzymatic activities of Renilla (Renilla reniformis) luciferase, firefly (Photinus pyralis) luciferase, and β-galactosidase, and showed comparable or greater stabilization of these enzymes than bovine serum albumin (BSA), a well-known stabilizer of enzyme activities. In particular, AgsA revealed better stabilization of Renilla luciferase and β-galactosidase than BSA under disulfide bond-reducing conditions with dithiothreitol. In addition, AgsA also increased the enzymatic performance of β-galactosidase and various restriction enzymes to a comparable or greater extent than BSA. These data indicate that AgsA may be useful as a general stabilizer of enzyme activities.
authors.affiliation: Department of Biological Science and Technology, Institute of Technology and Science, The University of Tokushima Graduate School, Minami-Josanjima-cho, Tokushima 770-8506, Japan. tomoyasu@bio.tokushima-u.ac.jp
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Tomoyasu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Tabata
authors.forename: Yoko
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Robert A
authors.initials: RA
authors.lastname: Whiley
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagamune
mesh_terms: D000818:Animals; D004220:Disulfides / Q000378:metabolism; D004229:Dithiothreitol / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D050888:Heat-Shock Proteins, Small / Q000378:metabolism* / Q000494:pharmacology; D006358:Hot Temperature; D049409:Luciferases, Firefly / Q000378:metabolism; D049410:Luciferases, Renilla / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism* / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000378:metabolism / Q000494:pharmacology; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004220:Disulfides; D050888:Heat-Shock Proteins, Small; D018832:Molecular Chaperones; D012710:Serum Albumin, Bovine; D049410:Luciferases, Renilla; D049409:Luciferases, Firefly; D001616:beta-Galactosidase; D004229:Dithiothreitol