title: Correlation among antioxidant, antimicrobial, hemolytic, and antiproliferative properties of Leiothrix spiralis leaves extract.
abstract: The biological activities of a plant extract depend on a complex sum of individual properties including the antioxidant activity. Several biological activities protect against the harmful action of reactive oxygen species (ROS), and here we focused our attention on the relationship between the biological activities tested and the antioxidant properties. In this study, the total flavonoid content as well as the antioxidant, antimicrobial, hemolytic and cytotoxicity activities of the methanolic extract of Leitothrix spiralis leaves were evaluated. The extract showed a total flavonoid content of 19.26% and the chemical characterization by HPLC-PAD confirmed the presence of flavonoids as the major secondary metabolite compounds. Significant antioxidant activity (IC(50) = 1.743 μg/mL ± 0.063) was demonstrated and was effective against Gram-negative organisms and all Candida strains tested, and showed an ability to inhibit hyphal formation. Non-hemolytic and antiproliferative activity could be demonstrated.
authors.affiliation: Biological Sciences Department, Faculty of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, SP 14801-902, Brazil.
authors.forename: Marcelo Gonzaga
authors.initials: MG
authors.lastname: De Freitas Araújo
authors.affiliation: Organic Chemistry Department, Chemistry Institute, São Paulo State University-UNESP, c.p. 355, Araraquara, SP 14800-900, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Hilário
authors.affiliation: Organic Chemistry Department, Chemistry Institute, São Paulo State University-UNESP, c.p. 355, Araraquara, SP 14800-900, Brazil.
authors.forename: Wagner
authors.identifier: 0000-0003-3032-2556
authors.initials: W
authors.lastname: Vilegas
authors.affiliation: Organic Chemistry Department, Chemistry Institute, São Paulo State University-UNESP, c.p. 355, Araraquara, SP 14800-900, Brazil.
authors.forename: Lourdes Campaner
authors.initials: LC
authors.lastname: Dos Santos
authors.affiliation: Clinical Analysis Department, Faculty of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, SP 14801-902, Brazil.
authors.forename: Iguatemy Lourenço
authors.initials: IL
authors.lastname: Brunetti
authors.affiliation: Department of Clinical Biochemistry, CIBICI-CONICET, Faculty of Chemical Sciences, National University of Cordoba, Haya de la Torre y Medina Allende, Córdoba 5000, Argentina.
authors.forename: Claudia Elena
authors.initials: CE
authors.lastname: Sotomayor
authors.affiliation: Biological Sciences Department, Faculty of Pharmaceutical Sciences, São Paulo State University-UNESP, Araraquara, SP 14801-902, Brazil.
authors.forename: Tais Maria
authors.initials: TM
authors.lastname: Bauab
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000254:growth & development*; D039262:Eriocaulaceae / Q000737:chemistry*; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000254:growth & development*; D006367:HeLa Cells; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts