title: Exposure to acute electromagnetic radiation of mobile phone exposure range alters transiently skin homeostasis of a model of pigmented reconstructed epidermis.
abstract: Exposure to electromagnetic radiations (EMR) produced by mobile phone concerns half the world's population and raises the problem of their impact on human health. In this study, we looked at the effects of mobile phone exposure (GSM basic, 900 MHz, SAR 2 mW g(-1) , 6 h) on a model of pigmented skin. We have analysed the expression and localization of various markers of keratinocyte and melanocyte differentiation 2, 6, 18 and 24 h after EMR exposure of reconstructed epidermis containing either only keratinocytes or a combination of keratinocytes and melanocytes grown on dead de-epidermized dermis, using histology, immunohistochemistry and Western blot. No changes were found in epidermal architecture, localization of epidermal markers, presence of apoptotic cells and the induction of p53 in both types of epidermis (with or without melanocytes) after exposure to EMR. In pigmented reconstructs, no change in the location and dendricity of melanocytes and in melanin transfer to neighbouring keratinocytes was detected after EMR exposure. Loricrin, cytokeratin 14 were significantly decreased at 6 h. The level of all markers increased at 24 h as compared to 6 h post-EMR exposure, associated with a significant decrease of the 20S proteasome activity. Our data indicate that exposure to 900 MHz frequency induces a transient alteration of epidermal homoeostasis, which may alter the protective capacity of the skin against external factors. Presence or absence of melanocytes did not modify the behaviour of reconstructs after EMR exposure.
authors.affiliation: University Bordeaux Segalen, 146 rue Leo Saignat, Bordeaux, France.
authors.forename: D
authors.initials: D
authors.lastname: Simon
authors.forename: A
authors.initials: A
authors.lastname: Daubos
authors.forename: C
authors.initials: C
authors.lastname: Pain
authors.forename: R
authors.initials: R
authors.lastname: Fitoussi
authors.forename: K
authors.initials: K
authors.lastname: Vié
authors.forename: A
authors.initials: A
authors.lastname: Taieb
authors.forename: L
authors.initials: L
authors.lastname: de Benetti
authors.forename: M
authors.initials: M
authors.lastname: Cario-André
mesh_terms: D017209:Apoptosis; D002454:Cell Differentiation; D040421:Cell Phone; D049109:Cell Proliferation; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D004817:Epidermis / Q000503:physiopathology*; D006706:Homeostasis; D006801:Humans; D007120:Immunochemistry; D008954:Models, Biological; D012880:Skin Pigmentation