title: Dynamic proteomic profile of potato tuber during its in vitro development.
abstract: Potato tuberization is a complicated biochemical process, which is dependent on external environmental factors. Tuber development in potato consists of a series of biochemical and morphological processes at the stolon tip. Signal transduction proteins are involved in the source-sink transition during potato tuberization. In the present study, we examined protein profiles under in vitro tuber-inducing conditions using a shotgun proteomic approach involving denaturing gel electrophoresis and liquid chromatography-mass spectrometry. A total of 251 proteins were identified and classified into 9 groups according to distinctive expression patterns during the tuberization stage. Stolon stage-specific proteins were primarily involved in the photosynthetic machinery. Proteins specific to the initial tuber stage included patatin. Proteins specific to the developing tuber stage included 6-fructokinase, phytoalexin-deficient 4-1, metallothionein II-like protein, and malate dehydrogenase. Novel stage-specific proteins identified during in vitro tuberization were ferredoxin-NADP reductase, 34 kDa porin, aquaporin, calmodulin, ripening-regulated protein, and starch synthase. Superoxide dismutase, dehydroascorbate reductase, and catalase I were most abundantly expressed in the stolon; however, the enzyme activities of these proteins were most activated at the initial tuber. The present shotgun proteomic study provides insights into the proteins that show altered expression during in vitro potato tuberization.
authors.affiliation: Department of Molecular Biotechnology, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Jae Woong
authors.initials: JW
authors.lastname: Yu
authors.forename: Jong-Soon
authors.initials: JS
authors.lastname: Choi
authors.forename: Chandrama Prakash
authors.initials: CP
authors.lastname: Upadhyaya
authors.forename: Sang Oh
authors.initials: SO
authors.lastname: Kwon
authors.forename: Mayank Anand
authors.initials: MA
authors.lastname: Gururani
authors.forename: Akula
authors.initials: A
authors.lastname: Nookaraju
authors.forename: Ju-Hyun
authors.initials: JH
authors.lastname: Nam
authors.forename: Chi-Won
authors.initials: CW
authors.lastname: Choi
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
authors.forename: Hemavathi
authors.initials: H
authors.lastname: Ajappala
authors.forename: Hyun Soon
authors.initials: HS
authors.lastname: Kim
authors.forename: Jae Heung
authors.initials: JH
authors.lastname: Jeon
authors.forename: Se Won
authors.initials: SW
authors.lastname: Park
mesh_terms: D063245:Plant Development; D010940:Plant Proteins / Q000378:metabolism*; D018547:Plant Stems / Q000254:growth & development / Q000378:metabolism; D035281:Plant Tubers / Q000254:growth & development / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction; D011198:Solanum tuberosum / Q000254:growth & development / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome