title: Proteomic differential display identifies upregulated vinculin as a             possible biomarker of pancreatic cancer.
abstract: Pancreatic cancer (PC) is characterized by rapid tumor spread, and very             few patients with PC survive for more than 5 years. It is imperative to discover             additional diagnostic biomarkers or specific therapeutic targets in order to improve             the treatment of patients with PC. In search for useful biomarkers, we analyzed             ten pairs of non-cancerous and cancer tissues from patients with PC by two-dimensional             gel electrophoresis (2-DE). Nineteen protein spots showed differential expression             on 2-DE gels between the cancer and non-cancerous tissues. Six upregulated protein             spots were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS)             as calreticulin, glutathione synthetase, stathmin, vinculin, α-enolase and glyceraldehyde-3-phosphate             dehydrogenase. Western blotting demonstrated that vinculin was predominantly expressed             in the pancreatic cancer tissues compared with to non-cancerous tissues. Our findings             indicate that vinculin may be a clinically useful biomarker of PC.
authors.affiliation: Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine, Ube, Japan.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kuramitsu
authors.forename: Tomio
authors.initials: T
authors.lastname: Ueno
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Suzuki
authors.forename: Shigefumi
authors.initials: S
authors.lastname: Yoshino
authors.forename: Norio
authors.initials: N
authors.lastname: Iizuka
authors.forename: Xiulian
authors.initials: X
authors.lastname: Zhang
authors.forename: Junko
authors.initials: J
authors.lastname: Akada
authors.forename: Masaaki
authors.initials: M
authors.lastname: Oka
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Nakamura
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D037282:Calreticulin / Q000032:analysis; D005260:Female; D020869:Gene Expression Profiling; D005981:Glutathione Synthase / Q000032:analysis; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D040901:Proteomics; D050777:Stathmin / Q000032:analysis; D015854:Up-Regulation; D016596:Vinculin / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D037282:Calreticulin; D050777:Stathmin; D016596:Vinculin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010751:Phosphopyruvate Hydratase; D005981:Glutathione Synthase