title: Non-adhesive behavior of new nanostructured PNIPAM surfaces towards specific neurodegenerative proteins: application to storage and titration of Tau proteins.
abstract: New nonfouling tubes are developed and their influence on the adhesion of neuroproteins is studied. The biomarkers are considered as single components (recombinant prion and Tau proteins) or in a solution of native and pathological forms. The samples are stored for 24 h at 4 °C in virgin and treated tubes layered with two different nanostructured coatings based on poly(N-isopropylacrylamide) with either a positive or a neutral charge, and the protein adhesion is monitored. The recombinant protein with a high pI is repelled from the nanostructured surface that has a negative ζ potential, whereas the recombinant protein with the lower pI is attracted. Furthermore, in the case of complex solutions, neutral nanostructured surfaces are able to retain all amyloid biomarkers.
authors.affiliation: Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, LUNAM Université, UMR Université du Maine - CNRS no. 6283, Avenue Olivier Messiaen, 72085 Le Mans, France.
authors.forename: Tjasa
authors.initials: T
authors.lastname: Vrlinic
authors.forename: Dominique
authors.initials: D
authors.lastname: Debarnot
authors.forename: Gilbert
authors.initials: G
authors.lastname: Legeay
authors.forename: Arnaud
authors.initials: A
authors.lastname: Coudreuse
authors.forename: Benaissa
authors.initials: B
authors.lastname: El Moualij
authors.forename: Willy
authors.initials: W
authors.lastname: Zorzi
authors.forename: Armand
authors.initials: A
authors.lastname: Perret-Liaudet
authors.forename: Isabelle
authors.initials: I
authors.lastname: Quadrio
authors.forename: Miran
authors.initials: M
authors.lastname: Mozetic
authors.forename: Fabienne
authors.initials: F
authors.lastname: Poncin-Epaillard
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D049329:Nanostructures; D010446:Peptide Fragments / Q000134:cerebrospinal fluid / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011108:Polymers / Q000737:chemistry*; D011328:Prions / Q000134:cerebrospinal fluid / Q000737:chemistry*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry; D012996:Solutions; D055672:Static Electricity; D013499:Surface Properties; D013816:Thermodynamics; D016875:tau Proteins / Q000134:cerebrospinal fluid / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D016229:Amyloid beta-Peptides; D020099:Coated Materials, Biocompatible; D010446:Peptide Fragments; D011108:Polymers; D011328:Prions; D011994:Recombinant Proteins; D012996:Solutions; C075222:amyloid beta-protein (1-42); D016875:tau Proteins; C052970:poly-N-isopropylacrylamide