title: Brachypodium distachyon as a model for defining the allergen potential of non-prolamin proteins.
abstract: Epitope databases and the protein sequences of published plant genomes are suitable to identify some of the proteins causing food allergies and sensitivities. Brachypodium distachyon, a diploid wild grass with a sequenced genome and low prolamin content, is the closest relative of the allergen cereals, such as wheat or barley. Using the Brachypodium genome sequence, a workflow has been developed to identify potentially harmful proteins which may cause either celiac disease or wheat allergy-related symptoms. Seed tissue-specific expression of the potential allergens has been determined, and intact epitopes following an in silico digestion with several endopeptidases have been identified. Molecular function of allergen proteins has been evaluated using Gene Ontology terms. Biologically overrepresented proteins and potentially allergen protein families have been identified.
authors.affiliation: Applied Genomics Department, Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Brunszvik 2, Martonvásár, Hungary. juhasz.angela@agrar.mta.hu
authors.forename: A
authors.initials: A
authors.lastname: Juhász
authors.forename: Gy
authors.initials: G
authors.lastname: Gell
authors.forename: E
authors.initials: E
authors.lastname: Sebestyén
authors.forename: R
authors.initials: R
authors.lastname: Haraszi
authors.forename: L
authors.initials: L
authors.lastname: Tamás
authors.forename: E
authors.initials: E
authors.lastname: Balázs
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D052179:Antigens, Plant / Q000737:chemistry / Q000276:immunology*; D058431:Brachypodium / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002446:Celiac Disease / Q000276:immunology; D032461:Chromosomes, Plant / Q000737:chemistry / Q000235:genetics; D030541:Databases, Genetic; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D020224:Expressed Sequence Tags; D018745:Genome, Plant; D006801:Humans; D018448:Models, Immunological; D055315:Prolamins / Q000737:chemistry; D055314:Seed Storage Proteins / Q000737:chemistry / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D014908:Triticum / Q000737:chemistry / Q000235:genetics / Q000276:immunology
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D000939:Epitopes; D055315:Prolamins; D055314:Seed Storage Proteins