title: Serum of Babesia rodhaini infected mice down regulates catalase activity of healthy erythrocytes.
abstract: Hemolytic activity for animals infected with various Babesia species is well reported. In this study, we confirmed that serum of Babesia rodhaini-infected mice also showed hemolytic activity. Erythrocytes from non-infected mice were lysed by co-incubation with B. rodhaini-infected serum. Catalase activity of the non-infected target erythrocytes was suppressed after this co-incubation with the hemolytic serum of B. rodhaini-infected mice. Furthermore, serum hemolytic activity was inhibited when target erythrocytes were incubated with hemolytic serum in the presence of exogenous catalase. Our study indicated that hemolytic serum can down-regulate the antioxidant capacity of non-infected healthy erythrocytes, possibly as a result of catalase activity, thereby leading to hemolysis.
authors.affiliation: United Graduate School of Veterinary Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Shih-Pin
authors.initials: SP
authors.lastname: Chiou
authors.forename: Naoaki
authors.initials: N
authors.lastname: Yokoyama
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Katsuya
authors.initials: K
authors.lastname: Kitoh
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takashima
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood; D001403:Babesia / Q000276:immunology; D001404:Babesiosis / Q000097:blood / Q000276:immunology*; D002374:Catalase / Q000378:metabolism*; D015536:Down-Regulation / Q000276:immunology; D004912:Erythrocytes / Q000201:enzymology* / Q000276:immunology; D005260:Female; D006461:Hemolysis / Q000276:immunology; D007962:Leukocytes / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D013481:Superoxides / Q000097:blood
chemical_list: D001323:Autoantibodies; D017382:Reactive Oxygen Species; D013481:Superoxides; D002374:Catalase