title: Protective effect of a polysaccharide from stem of Codonopsis pilosula against renal ischemia/reperfusion injury in rats.
abstract: In this study, we purified a homogeneous polysaccharide (S-CPPA1) with a molecular weight (Mw) of 133.2 kDa from the stem of Codonopsis pilosula for the first time. Gas chromatography (GC) analysis identified that S-CPPA1 contained glucose, galactose, and arabinose with a molar ratio of 10.5:3.4:1.7, along with a trace of mannose. Methylation analysis suggested S-CPPA1 was a branched polysaccharide, with five glucosidic linkage forms, namely (1→4)-linked Glcp (residue A), (1→6)-linked Galp (residue B), (1→2,6)-linked Glcp (residue C), (1→5)-linked Araf (residue D), and non-reducing terminal (1→)-linked Glcp (residue E). The protective effect of S-CPPA1 on kidney ischemia/reperfusion (I/R) injury was also evaluated. Blood urea nitrogen (BUN), creatinine and TNF-α levels, as well as lactate dehydrogenase (LDH) and alanine transaminase (AST) activities were elevated in the I/R group as compared to the sham group. On the other hand, S-CPPA1 treatment reversed all these biochemical indices, as well as histopathological alterations, which were induced by I/R. The findings imply that S-CPPA1 plays a causal role in the protection against I/R-induced renal injury and its renoprotective effect is probably mediated by inhibiting the proinflammatory cytokine TNF-α release.
authors.affiliation: Nephrology Division, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Zhanting
authors.initials: Z
authors.lastname: Li
authors.forename: Libing
authors.initials: L
authors.lastname: Zhu
authors.forename: Han
authors.initials: H
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Dewei
authors.initials: D
authors.lastname: Du
authors.forename: Junping
authors.initials: J
authors.lastname: Meng
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jifeng
authors.initials: J
authors.lastname: Sun
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001806:Blood Urea Nitrogen; D029745:Codonopsis / Q000737:chemistry*; D003404:Creatinine / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D018547:Plant Stems / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D000410:Alanine Transaminase