title: An experimental model for induction of lung cancer in rats by Chlamydia pneumoniae.
abstract: OBJECTIVE
  To assess induction effects of Chlamydia pneumoniae (Cpn) on lung cancer in rats.
  
  
  METHODS
  A lung cancer animal model was developed through repeated intratracheal injection of Cpn (TW-183) into the lungs of rats, with or without exposure to benzo(a)pyrene (Bp). Cpn antibodies (Cpn-IgA, -IgG, and -IgM) in serum were measured by microimmunofluorescence. Cpn-DNA or Cpn-Ag of rat lung cancer was detected through polymerase chain reaction or enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The prevalence of Cpn infection was 72.9% (35/48) in the Cpn group and 76.7% (33/43) in the Cpn plus benzo(a)pyrene (Bp) group, with incidences of lung carcinomas in the two groups of 14.6% (7/48) and 44.2% (19/43), respectively (P-values 0.001 and <0.001 compared with normal controls).
  
  
  CONCLUSIONS
  A rat model of lung carcinoma induced by Cpn infection was successfully established in the laboratory for future studies on the treatment, prevention, and mechanisms of the disease.
authors.affiliation: Department of Pulmonary Medicine, Jinshan Hospital, Fudan University, Shanghai, China. dejiechu@yeah.net
authors.forename: De-Jie
authors.initials: DJ
authors.lastname: Chu
authors.forename: Shui-Gen
authors.initials: SG
authors.lastname: Guo
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Yong
authors.initials: Y
authors.lastname: Du
authors.forename: Xu-Feng
authors.initials: XF
authors.lastname: Lu
authors.forename: Zhu-Yuan
authors.initials: ZY
authors.lastname: Yu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001580:Benzopyrenes; D023521:Chlamydophila Infections / Q000473:pathology*; D016993:Chlamydophila pneumoniae; D004195:Disease Models, Animal; D008175:Lung Neoplasms / Q000139:chemically induced / Q000382:microbiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000907:Antibodies, Bacterial; D001580:Benzopyrenes