title: Homing and engraftment of bone marrow cells derived from different donors in a murine model of sensitization.
abstract: Sensitized recipients are at a high risk of graft rejection in hematopoietic stem cell transplantation. To explore the trace of donor cells, we tried to explore homing and engraftment of bone marrow cells (BMCs) derived from different donors in a murine model of sensitization. Sensitized  BALB/c mice were used as transplanted recipients, which received BMCs derived from C57BL/6 or BALB/c donors after lethal irradiation. The homing study showed that the donor cells decreased along with time in recipients of the C57BL/6 donor group, but the donor cells increased along with time  in recipients of the BALB/c donor group. For the engraftment assay, all the sensitized recipients transplanted with BMCs derived from C57BL/6 donors died after lethal irradiation. In contrast, all the recipients transplanted with BMCs derived from BALB/c donors got long-term survival. Our  results suggest that it is crucial to have human leukocyte antigen identical donors for sensitized recipients during hematopoietic stem cell transplantation.
authors.affiliation: Department of Pediatrics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Lu-Hong
authors.initials: LH
authors.lastname: Xu
authors.forename: Jian-Pei
authors.initials: JP
authors.lastname: Fang
authors.forename: Hong-Gui
authors.initials: HG
authors.lastname: Xu
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Weng
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology*; D016026:Bone Marrow Transplantation / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology*; D016867:Immunocompromised Host / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016019:Survival Analysis; D014019:Tissue Donors; D014184:Transplantation, Homologous