title: Rapid determination of lymphogranuloma venereum serovars of Chlamydia trachomatis by quantitative high-resolution melt analysis (HRMA).
abstract: A quantitative high-resolution melt analysis assay was developed to differentiate lymphogranuloma venereum-causing serovars of Chlamydia trachomatis (L1 to L3) from other C. trachomatis serovars (D to K). The detection limit of this assay is approximately 10 copies per reaction, comparable to the limits of other quantitative-PCR-based methods.
authors.affiliation: Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia. jimmy.twin@mcri.edu.au
authors.forename: Jimmy
authors.initials: J
authors.lastname: Twin
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Stevens
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Garland
authors.forename: Angelo M
authors.initials: AM
authors.lastname: Zaia
authors.forename: Sepehr N
authors.initials: SN
authors.lastname: Tabrizi
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008219:Lymphogranuloma Venereum / Q000382:microbiology*; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D044366:Transition Temperature