title: A census of human soluble protein complexes.
abstract: Cellular processes often depend on stable physical associations between proteins. Despite recent progress, knowledge of the composition of human protein complexes remains limited. To close this gap, we applied an integrative global proteomic profiling approach, based on chromatographic separation of cultured human cell extracts into more than one thousand biochemical fractions that were subsequently analyzed by quantitative tandem mass spectrometry, to systematically identify a network of 13,993 high-confidence physical interactions among 3,006 stably associated soluble human proteins. Most of the 622 putative protein complexes we report are linked to core biological processes and encompass both candidate disease genes and unannotated proteins to inform on mechanism. Strikingly, whereas larger multiprotein assemblies tend to be more extensively annotated and evolutionarily conserved, human protein complexes with five or fewer subunits are far more likely to be functionally unannotated or restricted to vertebrates, suggesting more recent functional innovations.
authors.affiliation: Banting and Best Department of Medical Research, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada.
authors.forename: Pierre C
authors.initials: PC
authors.lastname: Havugimana
authors.forename: G Traver
authors.initials: GT
authors.lastname: Hart
authors.forename: Tamás
authors.initials: T
authors.lastname: Nepusz
authors.forename: Haixuan
authors.initials: H
authors.lastname: Yang
authors.forename: Andrei L
authors.initials: AL
authors.lastname: Turinsky
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Li
authors.forename: Peggy I
authors.initials: PI
authors.lastname: Wang
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Boutz
authors.forename: Vincent
authors.initials: V
authors.lastname: Fong
authors.forename: Sadhna
authors.initials: S
authors.lastname: Phanse
authors.forename: Mohan
authors.initials: M
authors.lastname: Babu
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Craig
authors.forename: Pingzhao
authors.initials: P
authors.lastname: Hu
authors.forename: Cuihong
authors.initials: C
authors.lastname: Wan
authors.forename: James
authors.initials: J
authors.lastname: Vlasblom
authors.forename: Vaqaar-un-Nisa
authors.initials: VU
authors.lastname: Dar
authors.forename: Alexandr
authors.initials: A
authors.lastname: Bezginov
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Clark
authors.forename: Gabriel C
authors.initials: GC
authors.lastname: Wu
authors.forename: Shoshana J
authors.initials: SJ
authors.lastname: Wodak
authors.forename: Elisabeth R M
authors.initials: ER
authors.lastname: Tillier
authors.forename: Alberto
authors.initials: A
authors.lastname: Paccanaro
authors.forename: Edward M
authors.initials: EM
authors.lastname: Marcotte
authors.forename: Andrew
authors.initials: A
authors.lastname: Emili
mesh_terms: D006801:Humans; D046912:Multiprotein Complexes / Q000032:analysis*; D060066:Protein Interaction Maps; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D046912:Multiprotein Complexes; D011506:Proteins