title: Molecular analysis of gut microbiota in obesity among Indian individuals.
abstract: Obesity is a consequence of a complex interplay between the host genome and the prevalent obesogenic factors among the modern communities. The role of gut microbiota in the pathogenesis of the disorder was recently discovered; however, 16S-rRNA-based surveys revealed compelling but community-specific data. Considering this, despite unique diets, dietary habits and an uprising trend in obesity, the Indian counterparts are poorly studied. Here, we report a comparative analysis and quantification of dominant gut microbiota of lean, normal, obese and surgically treated obese individuals of Indian origin. Representative gut microbial diversity was assessed by sequencing fecal 16S rRNA libraries for each group (n=5) with a total of over 3000 sequences. We detected no evident trend in the distribution of the predominant bacterial phyla, Bacteroidetes and Firmicutes. At the genus level, the bacteria of genus Bacteroides were prominent among the obese individuals, which was further confirmed by qPCR (P less than 0.05). In addition, a remarkably high archaeal density with elevated fecal SCFA levels was also noted in the obese group. On the contrary, the treated-obese individuals exhibited comparatively reduced Bacteroides and archaeal counts along with reduced fecal SCFAs. In conclusion, the study successfully identified a representative microbial diversity in the Indian subjects and demonstrated the prominence of certain bacterial groups in obese individuals; nevertheless, further studies are essential to understand their role in obesity.
authors.affiliation: National Centre for Cell Science, Ganeshkhind, Pune 411 007, India.
authors.forename: Deepak P
authors.initials: DP
authors.lastname: Patil
authors.forename: Dhiraj P
authors.initials: DP
authors.lastname: Dhotre
authors.forename: Sachin G
authors.initials: SG
authors.lastname: Chavan
authors.forename: Armiya
authors.initials: A
authors.lastname: Sultan
authors.forename: Dhawal S
authors.initials: DS
authors.lastname: Jain
authors.forename: Vikram B
authors.initials: VB
authors.lastname: Lanjekar
authors.forename: Jayshree
authors.initials: J
authors.lastname: Gangawani
authors.forename: Poonam S
authors.initials: PS
authors.lastname: Shah
authors.forename: Jayshree S
authors.initials: JS
authors.lastname: Todkar
authors.forename: Shashank
authors.initials: S
authors.lastname: Shah
authors.forename: Dilip R
authors.initials: DR
authors.lastname: Ranade
authors.forename: Milind S
authors.initials: MS
authors.lastname: Patole
authors.forename: Yogesh S
authors.initials: YS
authors.lastname: Shouche
mesh_terms: D000328:Adult; D001105:Archaea / Q000235:genetics / Q000302:isolation & purification; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D058491:Bacterial Load; D001439:Bacteroides / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D005243:Feces / Q000382:microbiology; D005247:Feeding Behavior; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D007194:India; D008297:Male; D054892:Metagenome; D008875:Middle Aged; D009765:Obesity / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D012336:RNA, Ribosomal, 16S