title: Effects of low frequency electromagnetic field on proliferation of human epidermal stem cells: An in vitro study.
abstract: To investigate the effects of low frequency electromagnetic fields (EMF) on the proliferation of epidermal stem cells, human epidermal stem cells (hESC) were isolated, expanded ex vivo, and then exposed to a low frequency EMF. The test and control cells were placed under the same environment. The test cells were exposed for 30 min/day to a 5 mT low frequency EMF at 1, 10, and 50 Hz for 3, 5, or 7 days. The effects of low frequency EMF on cell proliferation, cell cycle, and cell-surface antigen phenotype were investigated. Low frequency EMF significantly enhanced the proliferation of hESC in the culture medium in a frequency-dependent manner, with the highest cell proliferation rate at 50 Hz (P < 0.05). Exposure to a low frequency EMF significantly increased the percentage of cells at the S phase of the cell cycle, coupled with a decrease in the percentage of cells in the G1 phase (P < 0.05) but the effect was not frequency dependent. The percentage of CD29(+) /CD71(-) cells remained unchanged in the low frequency EMF-exposed hESC. The results suggested that low frequency EMF influenced hESC proliferation in vitro, and this effect was related to the increased proportion of cells at the S phase.
authors.affiliation: Department of Physical Medicine and Rehabilitation, Guangdong Geriatric Institute, Guangdong Academy of Medical Sciences and Guangdong General Hospital, Guangzhou, China. mszrch@163.com
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Zhang
authors.forename: Xinping
authors.initials: X
authors.lastname: Li
authors.forename: Liming
authors.initials: L
authors.lastname: Bai
authors.forename: Kenzo
authors.initials: K
authors.lastname: Uchida
authors.forename: Wenfang
authors.initials: W
authors.lastname: Bai
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.forename: Weicheng
authors.initials: W
authors.lastname: Xu
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000378:metabolism; D002453:Cell Cycle / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D002648:Child; D004574:Electromagnetic Fields / Q000009:adverse effects*; D000078404:Epidermal Cells; D006801:Humans; D008297:Male; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D055815:Young Adult
chemical_list: D015415:Biomarkers