title: Quantitative expression of C-type lectin receptors in humans and mice.
abstract: C-type lectin receptors and their adaptor molecules are involved in the recognition of glycosylated self-antigens and pathogens. However, little is known about the species- and organ-specific expression profiles of these molecules. We therefore determined the mRNA expression levels of Dectin-1, MR1, MR2, DC-SIGN, Syk, Card-9, Bcl-10, Malt-1, Src, Dec-205, Galectin-1, Tim-3, Trem-1, and DAP-12 in 11 solid organs of human and mice. Mouse organs revealed lower mRNA levels of most molecules compared to spleen. However, Dec-205 and Galectin-1 in thymus, Src in brain, MR2, Card-9, Bcl-10, Src, and Dec-205 in small intestine, MR2, Bcl-10, Src, Galectin-1 in kidney, and Src and Galectin-1 in muscle were at least 2-fold higher expressed compared to spleen. Human lung, liver and heart expressed higher mRNA levels of most genes compared to spleen. Dectin-1, MR1, Syk and Trem-1 mRNA were strongly up-regulated upon ischemia-reperfusion injury in murine kidney. Tim3, DAP-12, Card-9, DC-SIGN and MR2 were further up-regulated during renal fibrosis. Murine kidney showed higher DAP-12, Syk, Card-9 and Dectin-1 mRNA expression during the progression of lupus nephritis. Thus, the organ-, and species-specific expression of C-type lectin receptors is different between mice and humans which must be considered in the interpretation of related studies.
authors.affiliation: Medical Clinic and Policlinic IV, Nephrological Center, University of Munich, Munich 80336, Germany.
authors.forename: Maciej
authors.initials: M
authors.lastname: Lech
authors.affiliation: Medical Clinic and Policlinic IV, Nephrological Center, University of Munich, Munich 80336, Germany.
authors.forename: Heni Eka
authors.initials: HE
authors.lastname: Susanti
authors.affiliation: Medical Clinic and Policlinic IV, Nephrological Center, University of Munich, Munich 80336, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Römmele
authors.affiliation: Medical Clinic and Policlinic IV, Nephrological Center, University of Munich, Munich 80336, Germany.
authors.forename: Regina
authors.initials: R
authors.lastname: Gröbmayr
authors.affiliation: Medical Clinic and Policlinic IV, Nephrological Center, University of Munich, Munich 80336, Germany.
authors.forename: Roman
authors.initials: R
authors.lastname: Günthner
authors.affiliation: Medical Clinic and Policlinic IV, Nephrological Center, University of Munich, Munich 80336, Germany.
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Anders
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000235:genetics* / Q000378:metabolism; D005355:Fibrosis / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007674:Kidney Diseases / Q000235:genetics* / Q000378:metabolism; D037181:Lectins, C-Type / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D037181:Lectins, C-Type; D012333:RNA, Messenger