title: Crystallization and preliminary diffraction studies of SFC-1, a carbapenemase conferring antibiotic resistance.
abstract: SFC-1, a class A carbapenemase that confers antibiotic resistance, hydrolyzes the β-lactam rings of β-lactam antibiotics (carbapenems, cephalosporins, penicillins and aztreonam). SFC-1 presents an enormous challenge to infection control, particularly in the eradication of Gram-negative pathogens. As SFC-1 exhibits a remarkably broad substrate range, including β-lactams of all classes, the enzyme is a potential target for the development of antimicrobial agents against pathogens producing carbapenemases. In this study, SFC-1 was cloned, overexpressed, purified and crystallized. The SFC-1 crystal diffracted to 1.6 Å resolution and belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 65.8, b = 68.3, c = 88.8 Å. Two molecules are present in the asymmetric unit, with a corresponding V(M) of 1.99 Å(3) Da(-1) and a solvent content of 38.1%.
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Myoung-Ki
authors.initials: MK
authors.lastname: Hong
authors.forename: Jae Jin
authors.initials: JJ
authors.lastname: Lee
authors.forename: Xing
authors.initials: X
authors.lastname: Wu
authors.forename: Jin-Kwang
authors.initials: JK
authors.lastname: Kim
authors.forename: Byeong Chul
authors.initials: BC
authors.lastname: Jeong
authors.forename: Tan-Viet
authors.initials: TV
authors.lastname: Pham
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Kim
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D018360:Crystallography, X-Ray; D004352:Drug Resistance, Microbial; D012705:Serratia / Q000201:enzymology*; D001618:beta-Lactamases / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase