title: Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum.
abstract: Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism Corynebacterium glutamicum.
authors.affiliation: Institut für Bio- und Geowissenschaften, IBG-1: Biotechnologie, Forschungszentrum Jülich, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Hentschel
authors.forename: Cornelia
authors.initials: C
authors.lastname: Will
authors.forename: Nurije
authors.initials: N
authors.lastname: Mustafi
authors.forename: Andreas
authors.initials: A
authors.lastname: Burkovski
authors.forename: Nadine
authors.initials: N
authors.lastname: Rehm
authors.forename: Julia
authors.initials: J
authors.lastname: Frunzke
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D014644:Genetic Variation; D005822:Genetic Vectors; D008164:Luminescent Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D055550:Protein Stability; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D016601:RNA-Binding Proteins; C430328:small protein B; C054562:yellow fluorescent protein, Bacteria