title: [Effects of intracellular osmolality changes on the voltage-gated sodium channels currents of trigeminal ganglion neuron].
abstract: OBJECTIVE
  To evaluate the biomechanical effects of intracellular changes on the voltage-gated sodium channels (VGSCs) on trigeminal ganglion neuron (TRGN).
  
  
  METHODS
  TRGN cells were acutely isolated from the neonatal SD rats. The voltage-dependent currents of the VGSCs on these neurons were elicited and analyzed by whole-cell patch-clamp recordings and the intracellular anisotonicity stimuli was established by adjusting the content of pipette solution. The effects of hypo-(260 mOsm) and hypertonic (350 mOsm) osmolarity on the activation and inactivation kinetics of VGSCs on TRGN were evaluated, compared with the normal intracellular environment.
  
  
  RESULTS
  The results demonstrated that intracellular hypotonic stimuli could influence both the activation and inactivation characteristics of VGSCs currents, including the membrane potential at half inactivation (V0.5) of the G-V and inactivation curves had obvious statistics significance (P<0.05) between hypotonicity (260mOsm) and isotonicity (306mOsm). However, only inactivation properties changed under intracellular hypertonic effects, including inactivation rate and k value.
  
  
  CONCLUSIONS
  It suggests that the kinetics of VGSCs on TRGN can be modulated both by intracellular hypo- and hypertonic with different characteristics.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Jiefei
authors.initials: J
authors.lastname: Shen
authors.forename: Haiye
authors.initials: H
authors.lastname: Wang
authors.forename: Yingyi
authors.initials: Y
authors.lastname: Ma
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Liu
authors.forename: Shuyuan
authors.initials: S
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Du
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008564:Membrane Potentials; D009474:Neurons; D009994:Osmolar Concentration; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D015222:Sodium Channels; D012668:Trigeminal Ganglion
chemical_list: D015222:Sodium Channels