title: [Effects of perfluorooctane sulfonate (PFOS) exposure on vitellogenin mRNA level in zebrafish (Brachydanio rerio)].
abstract: To study the endocrine disrupting effects and action mechanism of environmental levels of perfluorooctane sulfonate (PFOS) on the aquatic species, the research for the effects of PFOS exposure on vitellogenin (VTG) mRNA level in livers of zebrafish (Brachydanio rerio) was conducted. Zebrafish were exposed to PFOS at four environmental low concentrations (0.1, 1, 10, 100 microg x L(-1)) for 21 days. Livers from male and female zebrafish were collected for RNA extraction, VTG1 and VTG3 mRNA levels were measured respectively using real-time polymerase chain reaction (RT-PCR). The results show that: 1) The VTG1 and VTG3 mRNA level in the livers of male zebrafish increased after PFOS exposure. The VTG1 mRNA level increased with a positive dose response pattern, with the maximum response at 100 microg x L(-1) PFOS exposure where a significant difference compared with the control was observed. The VTG3 mRNA level increased as an inverted U-shaped dose response pattern, indicated as hormesis effects, where significant differences compared with the control were observed at 10 and 100 microg x L(-1) PFOS exposure. 2) The VTG1 mRNA level in the livers of female zebrafish increased where a significant difference compared with the control was observed at 10 microg x L(-1) PFOS exposure, but the standard errors for mRNA level at 10 and 100 microg x L(-1) PFOS exposure were distinct. The VTG3 mRNA level in the livers of female zebrafish increased at 10 microg L(-1) PFOS exposure but had no significant difference compared with the control. Thus, it deduced that PFOS exposure could be active on the endocrine system of zebrafish with the oestrogenic simulation action mechanism, and the VTG1 and VTG3 mRNA level in the livers of zebrafish might be sensitive biomarkers for the endocrine disrupting effects evaluation after PFOS exposure, with different responding patterns related to the gene subtypes and sex.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China. yanzi77pku@yahoo.com.cn
authors.forename: Yan
authors.initials: Y
authors.lastname: Cheng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cui
authors.forename: Zhi-Chao
authors.initials: ZC
authors.lastname: Dang
authors.forename: Wen-Ping
authors.initials: WP
authors.lastname: Xie
authors.forename: Hai-Shan
authors.initials: HS
authors.lastname: Li
authors.forename: Huan-Huan
authors.initials: HH
authors.lastname: Yin
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Chen
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D052244:Endocrine Disruptors / Q000633:toxicity; D004781:Environmental Exposure / Q000009:adverse effects*; D005260:Female; D005466:Fluorocarbons / Q000633:toxicity*; D008099:Liver / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D017738:Alkanesulfonic Acids; D052244:Endocrine Disruptors; D005466:Fluorocarbons; D012333:RNA, Messenger; D014819:Vitellogenins; C076994:perfluorooctane sulfonic acid