title: The mitochondrial genome of the quiet-calling katydids, Xizicus fascipes (Orthoptera: Tettigoniidae: Meconematinae).
abstract: To help determine whether the typical arthropod arrangement was a synapomorphy for the whole Tettigoniidae, we sequenced the mitochondrial genome (mitogenome) of the quiet-calling katydids, Xizicus fascipes (Orthoptera: Tettigoniidae: Meconematinae). The 16,166-bp nucleotide sequences of X. fascipes mitogenome contains the typical gene content, gene order, base composition, and codon usage found in arthropod mitogenomes. As a whole, the X. fascipes mitogenome contains a lower A+T content (70.2%) found in the complete orthopteran mitogenomes determined to date. All protein-coding genes started with a typical ATN codon. Ten of the 13 protein-coding genes have a complete termination codon, but the remaining three genes (COIII, ND5 and ND4) terminate with incomplete T. All tRNAs have the typical clover-leaf structure of mitogenome tRNA, except for tRNA(Ser(AGN)), in which lengthened anticodon stem (9 bp) with a bulged nuleotide in the middle, an unusual T-stem (6 bp in constrast to the normal 5 bp), a mini DHU arm (2 bp) and no connector nucleotides. In the A+T-rich region, two (TA)n conserved blocks that were previously described in Ensifera and two 150-bp tandem repeats plus a partial copy of the composed at 61 bp of the beginning were present. Phylogenetic analysis found: i) the monophyly of Conocephalinae was interrupted by Elimaea cheni from Phaneropterinae; and ii) Meconematinae was the most basal group among these five subfamilies.
authors.affiliation: College of Life Sciences, Hebei University, Baoding 071002, People's Republic of China.
authors.forename: Ming Ru
authors.initials: MR
authors.lastname: Yang
authors.forename: Zhi Jun
authors.initials: ZJ
authors.lastname: Zhou
authors.forename: Yan Lin
authors.initials: YL
authors.lastname: Chang
authors.forename: Le Hong
authors.initials: le H
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D017124:Conserved Sequence; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D005260:Female; D054629:Genome, Mitochondrial; D006135:Gryllidae / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics; D024101:Mitochondrial Proteins / Q000235:genetics; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012362:RNA, Transfer, Ser / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D019476:Insect Proteins; D024101:Mitochondrial Proteins; D012335:RNA, Ribosomal; D012362:RNA, Transfer, Ser; D009245:NADH Dehydrogenase; D025261:Mitochondrial Proton-Translocating ATPases