title: The activity of phosphoinositide-specific phospholipase C is required for vegetative growth and cell wall regeneration in Coprinopsis cinerea.
abstract: Three isotypes of phosphoinositide-specific phospholipase C designated CcPLC1, CcPLC2, and CcPLC3 were identified in Coprinopsis cinerea, through a search of the genome sequence database. The functional role of the PI-PLCs were studied by using U73122, which specifically inhibits the activity of PI-PLC. The specificity of the inhibitor effect was confirmed by using an inactive structural analog U73433. The inhibition of PI-PLCs activity resulted in severely retarded germination of basidiospores and oidia, reduced hyphal growth, knobbly hyphal tips with many irregular side branches, and aberrant (branch-like structure) clamp cells. Furthermore, U73122 definitely inhibited cell wall formation. Here we report that PI-PLCs play important roles in various aspects of C. cinerea biology.
authors.affiliation: Department of Microbiology, College of Natural Science/Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Young Taek
authors.initials: YT
authors.lastname: Oh
authors.forename: Chun-Seob
authors.initials: CS
authors.lastname: Ahn
authors.forename: Kyung-Jin
authors.initials: KJ
authors.lastname: Lee
authors.forename: Jeong-Geun
authors.initials: JG
authors.lastname: Kim
authors.forename: Hyeon-Su
authors.initials: HS
authors.lastname: Ro
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Kim
authors.forename: Chang-Won
authors.initials: CW
authors.lastname: Lee
mesh_terms: D000363:Agaricales / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D002473:Cell Wall / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D004791:Enzyme Inhibitors / Q000378:metabolism; D004963:Estrenes / Q000378:metabolism; D016681:Genome, Fungal; D025301:Hyphae / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D010716:Phosphatidylinositols / Q000378:metabolism*; D011760:Pyrrolidinones / Q000378:metabolism; D012038:Regeneration; D013172:Spores, Fungal / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D004963:Estrenes; D010716:Phosphatidylinositols; D011760:Pyrrolidinones; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D010738:Type C Phospholipases