title: Enhanced histamine production through the induction of histidine decarboxylase             expression by phorbol ester in Jurkat cells.
abstract: Histamine (HA), a mediator of inflammation, type I allergic responses and             neurotransmission, is synthesized from L-histidine, the reaction of which is catalyzed             by histidine decarboxylase (HDC). HDC has been reported to be induced by various             stimuli, not only in mast cells and basophils, but also in T lymphocytes and macrophages.             Although its mRNA has been shown to be increased in Jurkat cells when treated             with phorbol 12-myristate 13-acetate (TPA), little is known concerning the induced             production of HA by HDC. The present study quantified the trace amounts of intracellular             HA using ultra-high liquid chromatography in combination with the 6-aminoquinoline             carbamate-derivatization technique. To test whether the cellular level of HA is             elevated by the induction of HDC in Jurkat cells treated with TPA, the peak corresponding             to authentic HA in the cell lysate was fractioned and its molecular weight determined             by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight             mass spectrometry. The results of this study show that the HA level is increased             by the induction of HDC expression by TPA in Jurkat cells. Therefore, this method             is useful in elucidating the physiological significance of HA production.
authors.affiliation: Graduate School of Life and Environmental Sciences, Faculty of Life and Environmental Sciences, Life Science Center of Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nagashima
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kako
authors.forename: Jun-Dal
authors.initials: JD
authors.lastname: Kim
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Fukamizu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006632:Histamine / Q000378:metabolism*; D006640:Histidine Decarboxylase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D011804:Quinolines / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C507665:6-aminosulfonylquinoline-4-carboxamide; D011804:Quinolines; D012333:RNA, Messenger; D006632:Histamine; D006640:Histidine Decarboxylase; D013755:Tetradecanoylphorbol Acetate