title: Maternal moderate physical training during pregnancy attenuates the effects of a low-protein diet on the impaired secretion of insulin in rats: potential role for compensation of insulin resistance and preventing gestational diabetes mellitus.
abstract: The effects of pregestational and gestational low-to-moderate physical training on insulin secretion in undernourished mothers were evaluated. Virgin female Wistar rats were divided into four groups as follows: control (C, n = 5); trained (T, n = 5); low-protein diet (LP, n = 5); trained with a low-protein diet (T + LP, n = 5). Trained rats ran on a treadmill over a period of 4 weeks before mate (5 days week⁻¹ and 60 min day⁻¹, at 65% of VO(2max)). At pregnancy, the intensity and duration of the exercise were reduced. Low-protein groups were provided with an 8% casein diet, and controls were provided with a 17% casein diet. At third day after delivery, mothers and pups were killed and islets were isolated by collagenase digestion of pancreas and incubated for a further 1 h with medium containing 5.6 or 16.7 mM glucose. T mothers showed increased insulin secretion by isolated islets incubated with 16.7 mM glucose, whereas LP group showed reduced secretion of insulin by isolated islets when compared with both C and LP + T groups. Physical training before and during pregnancy attenuated the effects of a low-protein diet on the secretion of insulin, suggesting a potential role for compensation of insulin resistance and preventing gestational diabetes mellitus.
authors.affiliation: Department of Nutrition, Federal University of Pernambuco, 50670-901 Recife, PE, Brazil. carolleandro22@gmail.com
authors.forename: Carol Góis
authors.initials: CG
authors.lastname: Leandro
authors.forename: Marco
authors.initials: M
authors.lastname: Fidalgo
authors.forename: Adriano
authors.initials: A
authors.lastname: Bento-Santos
authors.forename: Filippe
authors.initials: F
authors.lastname: Falcão-Tebas
authors.forename: Diogo
authors.initials: D
authors.lastname: Vasconcelos
authors.forename: Raul
authors.initials: R
authors.lastname: Manhães-de-Castro
authors.forename: Angelo Rafael
authors.initials: AR
authors.lastname: Carpinelli
authors.forename: Sandro Massao
authors.initials: SM
authors.lastname: Hirabara
authors.forename: Rui
authors.initials: R
authors.lastname: Curi
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D016640:Diabetes, Gestational / Q000378:metabolism* / Q000517:prevention & control*; D018753:Diet, Protein-Restricted / Q000009:adverse effects*; D005247:Feeding Behavior / Q000187:drug effects; D005260:Female; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D007333:Insulin Resistance; D000078790:Insulin Secretion; D008098:Litter Size / Q000187:drug effects; D010805:Physical Conditioning, Animal; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D007328:Insulin; D005947:Glucose