title: [Screening of differentially expressed serum proteins in patients with asbestosis].
abstract: OBJECTIVE
  To screen differently expressed proteins for serum biomarkers by studying serum proteome of population with asbestosis, population exposed to asbestos without asbestosis and population never exposed to asbestos, to further understand the mechanisms of asbestosis.
  
  
  METHODS
  The subjects of present study included 37 patients with asbestosis, 254 workers exposed to asbestos and 439 healthy controls. The 2-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI-TOF-MS/MS) were used to screen and identify the differentially expressed serum proteins among all subjects. ImageMaster6.0 software was utilized to analyze the differentially expressed proteins.
  
  
  RESULTS
  Well-qualified gel images of serum proteome were obtained, 21, 34 and 32 differentially expressed spots were found between asbestosis and normal controls, between asbestosis and negative controls or between negative controls and normal controls, respectively. Differentially displayed proteins were identified as cytokines, α1-AT, L-ficolin, etc.
  
  
  CONCLUSIONS
  Exposure to asbestos for a long period could interfere with the immune system of workers exposed to asbestos, and some proteins may serve as the biomarkers for early diagnosis and intervention of asbestosis.
authors.affiliation: Institute of Hygiene, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ju
authors.forename: Jun-qiang
authors.initials: JQ
authors.lastname: Chen
authors.forename: Zhao-qiang
authors.initials: ZQ
authors.lastname: Jiang
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiao
authors.forename: Li-jin
authors.initials: LJ
authors.lastname: Zhu
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Jia
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000328:Adult; D001195:Asbestosis / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D040901:Proteomics
chemical_list: D015415:Biomarkers; D001798:Blood Proteins