title: Identification of the cancer/testis antigens AKAP3 and CTp11 by SEREX             in hepatocellular carcinoma.
abstract: Cancer/testis (CT) antigens are considered promising target molecules for immunotherapy. To efficiently identify potential CT antigens, a testis cDNA library was immunoscreened with sera from hepatocellular carcinoma (HCC) patients. We isolated 3 different antigens, AKAP3, CTp11, and UBQLN3. Although AKAP3 and CTp11 have been previously reported as CT antigens, this is the first time that these 2 antigens have been isolated from HCC patients by SEREX. Conventional RT-PCR analysis showed that AKAP3 was frequently present in HCC cell lines (5/7) and HCC tissues (5/10), and the gene was broadly expressed in several cancer types, including breast cancer cell lines (3/6), breast cancer tissues (6/9), colon cancer cell lines (3/10), colon cancer tissues (5/6), ovary cancer cell lines (6/8), ovary cancer tissues (11/16), lung cancer cell lines (4/7) and lung cancer tissues (6/13). By phage plaque analysis, anti-AKAP3 antibody was detected in sera from 15 of 27 HCC patients and 8 of 27 healthy donors. These data suggest that AKAP3 may be useful for diagnosis and immunotherapy in HCC patients.
authors.affiliation: Department of Biochemistry, School of Medicine, Pusan National University, Yangsan-si, Gyeongsangnam-do 626-770, Republic of Korea.
authors.forename: Myung-Ha
authors.initials: MH
authors.lastname: Song
authors.forename: Kyung-Un
authors.initials: KU
authors.lastname: Choi
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Shin
authors.forename: Chang-Hun
authors.initials: CH
authors.lastname: Lee
authors.forename: Sang-Yull
authors.initials: SY
authors.lastname: Lee
mesh_terms: D054758:A Kinase Anchor Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D000368:Aged; D000951:Antigens, Neoplasm / Q000097:blood* / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000276:immunology* / Q000628:therapy; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis / Q000276:immunology / Q000628:therapy; D008297:Male; D009363:Neoplasm Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D009369:Neoplasms / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D014452:Ubiquitins / Q000097:blood / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D054758:A Kinase Anchor Proteins; C119530:AKAP3 protein, human; D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C403097:SPANXC protein, human; C410825:UBQLN3 protein, human; D014452:Ubiquitins