title: Cytogenetic dose-response in vitro for biological dosimetry after exposure to high doses of gamma-rays.
abstract: The dose response for dicentrics plus centric rings and total unstable chromosome-type aberrations was studied in the first mitoses of cultured human peripheral blood lymphocytes irradiated in vitro to doses of ∼2, 4, 6, 8, 10, 16 and 20 Gy of acute (60)Со gamma-rays. A dose-dependent increase of aberration yield was accompanied by a tendency to the underdispersion of dicentrics and centric rings among cells distributions compared with Poisson statistics at doses ≥6 Gy. The formal fitting of the data to a linear-quadratic model resulted in an equation with the linear and quadratic coefficients ranged 0.098-0.129×cell(-1)×Gy(-1) and 0.039-0.034×cell(-1)×Gy(-2), respectively, depending on the fitting method. The actual radiation-induced aberration yield was markedly lower than expected from a calibration curve, generated earlier within a lower dose range. Interlaboratory variations in reported dicentric yields induced by medium-to-high radiation doses in vitro are discussed.
authors.affiliation: Radiation Cytogenetics Laboratory, Grigoriev Institute for Medical Radiology of the National Academy of Medical Science of Ukraine, Pushkinskaya St. 82, Kharkiv 61024, Ukraine. vlad.vinnikov@mail.ru
authors.forename: Volodymyr A
authors.initials: VA
authors.lastname: Vinnikov
authors.forename: Nataliya A
authors.initials: NA
authors.lastname: Maznyk
mesh_terms: D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D003037:Cobalt Radioisotopes / Q000009:adverse effects*; D020732:Cytogenetic Analysis; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects*; D008297:Male; D008875:Middle Aged; D008938:Mitosis / Q000528:radiation effects*; D011874:Radiometry
chemical_list: D003037:Cobalt Radioisotopes