title: The disruption of mitochondrial metabolism and ion homeostasis in chicken hearts exposed to manganese.
abstract: Exposure to high levels of manganese (Mn) can result in cardiotoxicity in animals. However, little is known about the effect of excess Mn on poultry hearts. The aim of this study was to investigate the effect of dietary Mn on chicken cardiac injuries and the possible mechanisms of this process. In the present study, 400 fifty-day-old Hy-line brown cocks were randomly divided into four groups, and were fed either a commercial diet (containing 100mg/kg Mn) or a Mn-supplemented diet containing 600mg/kg, 900mg/kg, or 1800mg/kg Mn for 30, 60 or 90 days, respectively. Next, we examined several biomarkers of cardiac injury, including biochemical blood serum analyses, electrocardiogram assays, histological analyses, ultra-structural assays and apoptosis assays. To investigate the possible mechanisms of Mn-induced cardiotoxicity, we examined the effect of MnCl(2) on mitochondrial function and metal ion homeostasis. We found that subchronic MnCl(2) exposure induced damage in chicken hearts. Further investigations indicated that possible mechanisms for Mn-induced chicken cardiac injury included the disruption of mitochondrial metabolism and the alteration of ion homeostasis.
authors.affiliation: Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
authors.forename: Jing-Jun
authors.initials: JJ
authors.lastname: Shao
authors.forename: Hai-Dong
authors.initials: HD
authors.lastname: Yao
authors.forename: Zi-Wei
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Shi-Wen
authors.initials: SW
authors.lastname: Xu
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002645:Chickens; D003402:Creatine Kinase / Q000097:blood; D004562:Electrocardiography / Q000662:veterinary; D004573:Electrolytes / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D006651:Histocytochemistry / Q000662:veterinary; D020287:In Situ Nick-End Labeling / Q000662:veterinary; D008297:Male; D008345:Manganese / Q000633:toxicity*; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism*; D011897:Random Allocation; D020107:Troponin T / Q000097:blood
chemical_list: D004573:Electrolytes; D020107:Troponin T; D008345:Manganese; D003402:Creatine Kinase; D000251:Adenosine Triphosphatases