title: [Expression of Na+/H+ antiporter gene KsNHX1 in Kochia sieversiana under saline-alkali stress].
abstract: The 3 'cDNA fragment of KsNHX1 in Kochia sieversiana was obtained by RACE. The phylogenetic analysis of antiporters from different plant species indicated that the KsNHX1 was a gene encoding vacuolar Na+/H+ antiporter. By using semi-quantitative RT-PCR, the expression profiles of the KsNHX1 under different saline-alkali stresses were examined. It was observed that the transcript of KsNHX1 in leaves under the stress of 200 mmol x L(-1) for 2-24 h increased gradually with increasing duration, and the expression of KsNHX1 in roots, stems, leaves, and flowers under the stress of 200 mmol x L(-1) for 10 h was up-regulated. Under various NaCl concentrations, the expression of KsNHX1 in leaves was up-regulated, with the maximum under 160 mmol x L(-1) of NaCl. The expression of KsNHX1 in roots was also up-regulated under the stress of < 400 mmol x L(-1). Under various concentrations of Na2CO3, the expression of KsNHX1 in roots appeared the similar change patterns with those under corresponding concentrations of NaCl, but the expression of KsNHX1 in leaves was down-regulated except under 160 mmol x L(-1). The changes of the expression patterns implied that KsNHX1 played roles in maintaining the homeostasis of K+/Na+, which probably contributed to the saline-alkali tolerance of Kochia sieversiana.
authors.affiliation: College of Life Sciences and Technology, Harbin Normal University, Harbin 150025, China. kinglikefys@126.com
authors.forename: Yin-Sheng
authors.initials: YS
authors.lastname: Fu
authors.forename: Ji-Zhe
authors.initials: JZ
authors.lastname: Cui
authors.forename: Guang-Dong
authors.initials: GD
authors.lastname: Chen
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Xiao-Ju
authors.initials: XJ
authors.lastname: Mi
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D019663:Chenopodiaceae / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D055049:Salt Tolerance / Q000235:genetics; D055051:Salt-Tolerant Plants / Q000235:genetics*; D017693:Sodium Bicarbonate / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics*; D014617:Vacuoles / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D017923:Sodium-Hydrogen Exchangers; D012965:Sodium Chloride; D017693:Sodium Bicarbonate