title: Nuclear microsatellites for Pinus pinea (Pinaceae), a genetically depauperate tree, and their transferability to P. halepensis.
abstract: OBJECTIVE
  Pinus pinea is one of the few widespread plant species that are also genetically depauperate. It is also an important commercial species with high market value seeds. A deeper knowledge of the existing population genetic variation was needed.
  
  
  RESULTS
  Twelve nuclear microsatellites were isolated from genomic and cDNA sequences and screened for variability in 729 individuals from 33 natural populations. Low level of genetic variability was confirmed with average expected heterozygosity of 0.11. Hardy-Weinberg equilibrium expectations were not met in only ∼10% of the possible locus/population combinations. All loci were in linkage equilibrium, and the frequency of null alleles was very low (≤1% in 332 out of 396 locus/population combinations). Nine out of the 12 microsatellites were successfully transferred to P. halepensis.
  
  
  CONCLUSIONS
  Despite low polymorphism, these new markers will be useful to resolve population structure and hold potential for seed origin identification and traceability.
authors.affiliation: Plant Genetics Institute, National Research Council, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy.
authors.forename: F
authors.initials: F
authors.lastname: Pinzauti
authors.forename: F
authors.initials: F
authors.lastname: Sebastiani
authors.forename: K B
authors.initials: KB
authors.lastname: Budde
authors.forename: B
authors.initials: B
authors.lastname: Fady
authors.forename: S C
authors.initials: SC
authors.lastname: González-Martínez
authors.forename: G G
authors.initials: GG
authors.lastname: Vendramin
mesh_terms: D002467:Cell Nucleus / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D028223:Pinus / Q000235:genetics*; D018401:Sample Size; D014170:Transformation, Genetic; D014197:Trees / Q000235:genetics*
chemical_list: D017931:DNA Primers