title: Estimation of paracrine signaling using quantitative RT-PCR from multiple patchy lesion samples.
abstract: We investigated whether correlations between mRNA levels of cytokines versus other proteins from patchy lesion could estimate cytokine paracrine signaling in vivo. Experiments with rat experimental autoimmune myocarditis (EAM), a patchy myocarditis model, indicated IL-1 and other protein levels were correlated, indicating paracrine signaling pathways in vivo.
authors.affiliation: Division of Cardiology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Shuang
authors.initials: S
authors.lastname: Jiao
authors.forename: Haruo
authors.initials: H
authors.lastname: Hanawa
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Suzuki
authors.forename: Ying Hua
authors.initials: YH
authors.lastname: Hu
authors.forename: Kaori
authors.initials: K
authors.lastname: Yoshida
authors.forename: Minako
authors.initials: M
authors.lastname: Ogura
authors.forename: Yukako
authors.initials: Y
authors.lastname: Ohno
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ito
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kashimura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Obata
authors.forename: Makoto
authors.initials: M
authors.lastname: Kodama
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D007375:Interleukin-1 / Q000235:genetics; D009205:Myocarditis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D019899:Paracrine Communication / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger