title: Effects of rice bran oil on the intestinal microbiota and metabolism of isoflavones in adult mice.
abstract: This study examined the effects of rice bran oil (RBO) on mouse intestinal microbiota and urinary isoflavonoids. Dietary RBO affects intestinal cholesterol absorption. Intestinal microbiota seem to play an important role in isoflavone metabolism. We hypothesized that dietary RBO changes the metabolism of isoflavonoids and intestinal microbiota in mice. Male mice were randomly divided into two groups: those fed a 0.05% daidzein with 10% RBO diet (RO group) and those fed a 0.05% daidzein with 10% lard control diet (LO group) for 30 days. Urinary amounts of daidzein and dihydrodaidzein were significantly lower in the RO group than in the LO group. The ratio of equol/daidzein was significantly higher in the RO group (p < 0.01) than in the LO group. The amount of fecal bile acids was significantly greater in the RO group than in the LO group. The composition of cecal microbiota differed between the RO and LO groups. The occupation ratios of Lactobacillales were significantly higher in the RO group (p < 0.05). Significant positive correlation (r = 0.591) was observed between the occupation ratios of Lactobacillales and fecal bile acid content of two dietary groups. This study suggests that dietary rice bran oil has the potential to affect the metabolism of daidzein by altering the metabolic activity of intestinal microbiota.
authors.affiliation: National Food Research Institute, the National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Motoi
authors.initials: M
authors.lastname: Tamura
authors.affiliation: National Food Research Institute, the National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Hori
authors.affiliation: National Food Research Institute, the National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Chigusa
authors.initials: C
authors.lastname: Hoshi
authors.affiliation: National Food Research Institute, the National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D000818:Animals; D002432:Cecum / Q000382:microbiology; D002784:Cholesterol / Q000097:blood; D004269:DNA, Bacterial / Q000235:genetics; D005243:Feces / Q000737:chemistry; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects / Q000382:microbiology*; D007529:Isoflavones / Q000378:metabolism* / Q000652:urine; D008055:Lipids / Q000737:chemistry; D008297:Male; D051379:Mice; D064307:Microbiota / Q000187:drug effects* / Q000235:genetics; D010938:Plant Oils / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D000073879:Rice Bran Oil; D014280:Triglycerides / Q000097:blood
chemical_list: D004269:DNA, Bacterial; D007529:Isoflavones; D008055:Lipids; D010938:Plant Oils; D014280:Triglycerides; D002784:Cholesterol; D000073879:Rice Bran Oil