title: Local orientational disorder in peptide fibrils probed by a combination of residue-specific 13C-18O labelling, polarised infrared spectroscopy and molecular combing.
abstract: A novel combination of site-specific isotope labelling, polarised infrared spectroscopy and molecular combing reveals local orientational ordering in the fibril-forming peptide YTIAALLSPYSGGRADS. Use of (13)C-(18)O labelled alanine residues demonstrates that the N-terminal end of the peptide is incorporated into the cross-beta structure, while the C-terminal end shows orientational disorder.
authors.affiliation: Department of Chemistry, University of Reading, Whiteknights Campus, Reading RG6 6AD, UK.
authors.forename: José C
authors.initials: JC
authors.lastname: Rodríguez-Pérez
authors.forename: Ian W
authors.initials: IW
authors.lastname: Hamley
authors.forename: Sally L
authors.initials: SL
authors.lastname: Gras
authors.forename: Adam M
authors.initials: AM
authors.lastname: Squires
mesh_terms: D000409:Alanine / Q000737:chemistry; D002247:Carbon Isotopes; D007553:Isotope Labeling; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D010103:Oxygen Isotopes; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002247:Carbon Isotopes; D009842:Oligopeptides; D010103:Oxygen Isotopes; D000409:Alanine