title: Dried matrix on paper disks: the next generation DBS microsampling technique for managing the hematocrit effect in DBS analysis.
abstract: BACKGROUND
  The hematocrit effect is a hurdle for successful introduction of the dried blood spot (DBS) in a regulated environment. Recently, attempts were taken to overcome the hematocrit effect by whole-cut DBS analysis. This paper presents the next-generation whole-cut DBS; dried matrix on paper disks (DMPD).
  
  
  RESULTS
  DMPD eliminated the hematocrit effect and demonstrated better accuracy and precision than regular DBS with partial punching. Observed accuracy and precision were 6.0 and 2.3% for DMPD, respectively, and -10.4 and 17.1%, for DBS, respectively.
  
  
  CONCLUSIONS
  The DMPD technique performed better than regular DBS by eliminating the hematocrit effect related blood volume bias. Although this effect was not observed with DMPD, a systematic error of 6.0% was detected and further technical development of DMPD could improve the performance.
authors.affiliation: Universidad de los Andes, Faculty of Sciences, Department of Chemistry, Carrera 1 No 18A-10, Bogota, Colombia. rj.meesters@uniandes.edu.co
authors.forename: Roland Jw
authors.initials: RJ
authors.lastname: Meesters
authors.forename: Jamie
authors.initials: J
authors.lastname: Zhang
authors.forename: Nick A
authors.initials: NA
authors.lastname: van Huizen
authors.forename: Gero P
authors.initials: GP
authors.lastname: Hooff
authors.forename: Rob A
authors.initials: RA
authors.lastname: Gruters
authors.forename: Theo M
authors.initials: TM
authors.lastname: Luider
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods; D001800:Blood Specimen Collection / Q000295:instrumentation* / Q000379:methods; D059788:Dried Blood Spot Testing / Q000379:methods*; D006801:Humans; D019829:Nevirapine / Q000097:blood*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D019829:Nevirapine