title: Application of probe electrospray ionization mass spectrometry (PESI-MS) to clinical diagnosis: solvent effect on lipid analysis.
abstract: We have examined several combinations of solvents with the aim of optimizing the ionization conditions for molecular diagnosis of malignant tumours by PESI-MS. Although the best conditions may depend on the actual species in the sample, the optimal conditions for renal cell carcinoma (RCC) were achieved by using alcohols. PESI-MS successfully delineated the differential expression of phospholipids (PCs) and triacylglycerols (TAGs) in noncancerous and RCC tissues by using these solvent systems. This study paves the way for the application of PESI-MS in medical samples.
authors.affiliation: Clean Energy Research Center, University of Yamanashi, Kofu, Yamanashi, Japan.
authors.forename: Mridul Kanti
authors.initials: MK
authors.lastname: Mandal
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yoshimura
authors.forename: Lee Chuin
authors.initials: LC
authors.lastname: Chen
authors.forename: Zhan
authors.initials: Z
authors.lastname: Yu
authors.forename: Tadao
authors.initials: T
authors.lastname: Nakazawa
authors.forename: Ryohei
authors.initials: R
authors.lastname: Katoh
authors.forename: Hideki
authors.initials: H
authors.lastname: Fujii
authors.forename: Sen
authors.initials: S
authors.lastname: Takeda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nonami
authors.forename: Kenzo
authors.initials: K
authors.lastname: Hiraoka
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000438:Alcohols / Q000737:chemistry; D000818:Animals; D002292:Carcinoma, Renal Cell / Q000737:chemistry / Q000378:metabolism; D005663:Furans / Q000737:chemistry; D006801:Humans; D007680:Kidney Neoplasms / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D000438:Alcohols; D005663:Furans; D010743:Phospholipids; D012997:Solvents; D014280:Triglycerides; C018674:tetrahydrofuran; C032159:acetonitrile