title: Elongation factor 1β' gene from Spodoptera exigua: characterization and function identification through RNA interference.
abstract: Elongation factor (EF) is a key regulation factor for translation in many organisms, including plants, bacteria, fungi, animals and insects. To investigate the nature and function of elongation factor 1β' from Spodoptera exigua (SeEF-1β'), its cDNA was cloned. This contained an open reading frame of 672 nucleotides encoding a protein of 223 amino acids with a predicted molecular weight of 24.04 kDa and pI of 4.53. Northern blotting revealed that SeEF-1β' mRNA is expressed in brain, epidermis, fat body, midgut, Malpighian tubules, ovary and tracheae. RT-PCR revealed that SeEF-1β' mRNA is expressed at different levels in fat body and whole body during different developmental stages. In RNAi experiments, the survival rate of insects injected with SeEF-1β' dsRNA was 58.7% at 36 h after injection, which was significantly lower than three control groups. Other elongation factors and transcription factors were also influenced when EF-1β' was suppressed. The results demonstrate that SeEF-1β' is a key gene in transcription in S. exigua.
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Zhao
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Hong-Shuang
authors.initials: HS
authors.lastname: Guo
authors.affiliation: Zhejiang Institute of Subtropical Crops, Wenzhou, Zhejiang 325005, China.
authors.forename: Xiang-Li
authors.initials: XL
authors.lastname: Dang
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Shi-Gui
authors.initials: SG
authors.lastname: Wang
authors.affiliation: Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D055785:Gene Knockdown Techniques; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D018411:Spodoptera / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D019476:Insect Proteins; D020648:Peptide Elongation Factor 1; D012330:RNA, Double-Stranded