title: Development and evaluation of an immunochromatographic strip for rapid detection of porcine hemagglutinating encephalomyelitis virus.
abstract: BACKGROUND
  The incidence of PHE among pigs in many countries is on the rise, and it has caused great economic losses to the pig industry. Therefore, the development of a sensitive, specific, and easily-performed assay is crucial for the rapid detection and surveillance of PHE-CoV infection and transmission.
  
  
  RESULTS
  An immunochromatographic strip was developed for the detection of PHE-CoV. The colloidal gold-labeled MAb 4D4 was used as the detection reagent, and the MAb 1E2 and goat anti-mouse IgG coated the strip's test and control lines, respectively. The immunochromatographic strip was capable of specifically detecting PHE-CoV with a HA unit of 2 within 10 min. Storage of the strips at room temperature for 6 months or at 4°C for 12 months did not change their sensitivity or specificity. Using RT-PCR as a reference test, the relative specificity and sensitivity of the immunochromatographic strip were determined to be 100% and 97.78%, respectively. There was an excellent agreement between the results obtained by RT-PCR and the immunochromatographic strips (kappa = 0.976). Additionally, there was a strong agreement between the sandwich enzyme-linked immunosorbent assay (ELISA) and immunochromatographic strips (Kappa = 0.976). When the immunochromatographic strips were used for diagnosing PHE-CoV infection in the Jilin Province, the PHE-CoV-positive rate ranged from 61.54% in the Jilin district to 17.95% in the Songyuan district.
  
  
  CONCLUSIONS
  Based on its high specificity, sensitivity, and stability, the immunochromatographic strip would be suitable for on-site detection of PHE-CoV for surveillance and epidemiological purposes.
authors.affiliation: College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
authors.forename: Keyan
authors.initials: K
authors.lastname: Chen
authors.forename: Kui
authors.initials: K
authors.lastname: Zhao
authors.forename: Deguang
authors.initials: D
authors.lastname: Song
authors.forename: Wenqi
authors.initials: W
authors.lastname: He
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Chuanbo
authors.initials: C
authors.lastname: Zhao
authors.forename: Chengli
authors.initials: C
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity / Q000379:methods*; D017934:Coronavirus / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000821:virology*; D013997:Time Factors; D014730:Veterinary Medicine / Q000379:methods*; D014773:Virology / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal