title: [The relationship between c-fos gene and filamentous actin cytoskeleton in MG-63 osteoblasts under cyclic tensile stress].
abstract: OBJECTIVE
  To investigate the relationship between c-fos gene and filamentous actin (F-actin) in MG-63 osteoblasts under cyclic tensile stress.
  
  
  METHODS
  MG-63 osteoblasts were subjected to cyclic tensile stress (0.5 Hz, 2 000 microstrain) for 3, 6, and 12 h. The changes of c-fos gene were investigated by fluorescent quantitation polymerase chain reaction. Then the best loading time group was screened as the experimental group compared with 0 h group. The changes of F-actin and c-fos were investigated with or without cytochalasin D treatment.
  
  
  RESULTS
  Cyclic tensile stress induced high expression of c-fos mRNA, and peaked at 3 h. After loading, F-actin had a structure reorganization, but had no change in expression. After cytochalasin D treatment, the formation of stress fibers and the fluorescence intensity of F-actin cytoskeleton significantly reduced, meanwhile the c-fos mRNA expression was inhibited.
  
  
  CONCLUSIONS
  After loading, there is only structure reorganization for F-actin, and the expression has not any change. That means the remodeling F-actin is the existing one. F-actin reorganization is an important part in c-fos gene expression induced by stress.
authors.affiliation: Dept. of Orthodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
authors.forename: Anqing
authors.initials: A
authors.lastname: Du
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Sen
authors.initials: S
authors.lastname: Zhao
authors.forename: Weipeng
authors.initials: W
authors.lastname: Li
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D008841:Actin Cytoskeleton; D000199:Actins; D015638:Cytochalasin D; D003599:Cytoskeleton; D016762:Genes, fos; D006801:Humans; D008870:Microtubules; D010006:Osteoblasts; D012333:RNA, Messenger; D013314:Stress, Mechanical
chemical_list: D000199:Actins; D012333:RNA, Messenger; D015638:Cytochalasin D