title: Low-intensity pulsed ultrasound inhibits messenger RNA expression of matrix metalloproteinase-13 induced by interleukin-1β in chondrocytes in an intensity-dependent manner.
abstract: The effect of low-intensity pulsed ultrasound (LIPUS) on articular cartilage metabolism has been characterized. However, the effect of LIPUS intensity on articular cartilage degradation factors remains unknown. This study aimed to investigate the immediate effect of LIPUS at several intensities on cultured chondrocytes treated with interleukin-1β (IL-1β) to induce an inflammatory response and on articular cartilage explants. Cultured chondrocytes and articular cartilage explants were treated by LIPUS at intensities of 0, 7.5, 30 and 120 mW/cm(2) or 0, 27 and 67 mW/cm(2), respectively. mRNA analysis revealed that LIPUS inhibited induction of MMP13 mRNA expression by 100 pg/mL IL-1β in cultured chondrocytes in an intensity-dependent manner. LIPUS also inhibited MMP13 and MMP1 mRNA expression in articular cartilage explants. Our results indicate that LIPUS may potentially protect articular cartilage by inhibiting MMP mRNA expression in an intensity-dependent manner and should thus be considered a useful candidate for daily treatment of OA.
authors.affiliation: Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.forename: Tomoki
authors.initials: T
authors.lastname: Aoyama
authors.forename: Shoki
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Xiangkai
authors.initials: X
authors.lastname: Zhang
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Akiyama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kuroki
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019902:Chondrocytes / Q000502:physiology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005786:Gene Expression Regulation / Q000235:genetics / Q000528:radiation effects; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D011829:Radiation Dosage; D051381:Rats; D017208:Rats, Wistar; D013010:Sonication / Q000379:methods*; D013552:Swine
chemical_list: D053583:Interleukin-1beta; D012333:RNA, Messenger; D053509:Matrix Metalloproteinase 13