title: Fermented and enzymatic production of chitin/chitosan oligosaccharides by extracellular chitinases from Bacillus cereus TKU027.
abstract: Two chitinases, Chi I and Chi II, were purified from the culture supernatant of Bacillus cereus TKU027 with shrimp head powder (SHP) as the sole carbon/nitrogen source. The molecular masses of Chi I and Chi II determined using SDS-PAGE were approximately 65kDa and 63kDa, respectively. Chi I toward various surfactants showed high stability, such as SDS, Tween 20, Tween 40 and Triton X-100, and these surfactants were stimulator of Chi I chitinase activity. Concomitant with the production of Chi I and Chi II, chitin oligosaccharides were also observed in the culture supernatant, including chitobiose, chitotriose, chitotetrose and chitopentose at concentrations of 0.44mg/mL, 0.08mg/mL, 0.09mg/mL and 0.43mg/mL, respectively. Chitosan with 60% deacetylation was degraded by TKU027 crude enzyme to prepare chitooligosaccharides. MALDI-TOF MS analysis of the enzymatic hydrolyzates indicated that the products were mainly chitooligosaccharides with degree of polymerization (DP) in the 4-9 range.
authors.affiliation: Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan. sabulo@mail.tku.edu.tw
authors.forename: San-Lang
authors.initials: SL
authors.lastname: Wang
authors.forename: Chin-Pei
authors.initials: CP
authors.lastname: Liu
authors.forename: Tzu-Wen
authors.initials: TW
authors.lastname: Liang
mesh_terms: D001409:Bacillus cereus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D002688:Chitinases / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D009844:Oligosaccharides; D048271:Chitosan; D002688:Chitinases