title: G protein regulator 1 (GPR-1) localizes to cortical sites of artificial mechanical indentation in Caenorhabditis elegans zygotes.
abstract: Cytokinesis and spindle positioning require the cortical force regulator G Protein Regulator 1/2 (GPR-1/2). GPR-1/2 is thought to localize to sites of cortical force generation. Does GPR-1/2 also act as a sensor for mechanical stimulation? I mechanically stimulated the cortex by indenting it with a glass needle and observed the cortical localization of a YFP::GPR-1 transgene. I found that cortical YFP::GPR-1 accumulated at the site of mechanical indentation. This phenomenon occurred on most of the cortical areas except the site of prospective cytokinesis furrow formation. This result suggests that GPR-1/2 can sense mechanical properties of the cortex, which may be important for GPR-1/2 function regulating spindle positioning and cytokinesis.
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany. Henrik.Bringmann@mpibpc.mpg.de
authors.forename: Henrik
authors.initials: H
authors.lastname: Bringmann
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D017173:Caenorhabditis elegans / Q000166:cytology* / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D048749:Cytokinesis; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013314:Stress, Mechanical; D015053:Zygote / Q000166:cytology* / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; C475458:G protein regulator 1, C elegans; D011993:Recombinant Fusion Proteins