title: [Effects of hyaluronic acid on biomechanic performance of porcine acellular dermal matrix plus thin skin autograft after transplantation].
abstract: OBJECTIVE
  To explore the effects of hyaluronic acid (HA) on biomechanical properties for porcine acellular dermal matrix (PADM) plus thin skin autograft after transplantation.
  
  
  METHODS
  The dorsa of 10 Japanese white rabbits were symmetrically divided into four areas of A-D by random grouping. Full-thickness skin defects were created in Groups A-C while Group D was blank with normal skin. Operations were performed in Group A: implant with HA + PADM + thin skin autografts, Group B: implant with PADM + thin skin autografts and Group C: skin autografts group. Histological examination of specimen was performed at Day 56 postoperatively. And the biomechanical properties such as relaxation and stress-strain properties of grafts were recorded.
  
  
  RESULTS
  The structure of PADM was found to be basically intact by hematoxylin and eosin E dyeing in Groups A and B. In Group A, dense fiber structure could be observed. Lots of regularly arranged collagenous fibers and new blood capillaries were grown into the dermal matrix with sparsely distributed inflammatory cells. In Group B, acellular dermal matrix became clustered with a small amount of invaded fibroblasts. And there was a high expression of inflammatory cells. The biomechanic performances of transplanted skin were: Group A's curve was mostly close to that of Group D's, Group B's curve was the most further from that of Group D's (P = 0.001) and Group C's curve stayed between Groups A and B. Under the same strain, the stress of Groups A-D was (87 ± 8), (115 ± 9), (63 ± 7) and (81 ± 4) kPa respectively. No significant difference of stress existed between these two groups (P = 0.838). There was significant difference of stress between Groups B/C and D (P = 0.001 and P = 0.009).
  
  
  CONCLUSIONS
  Topical hyaluronic acid may be used to enhance the biomechanics performances of transplanted skin.
authors.affiliation: Department of Burns & Plastic Surgery, First Affiliated Hospital, General Hospital of PLA, Beijing 100048, China.
authors.forename: Jing-yu
authors.initials: JY
authors.lastname: Zhao
authors.forename: Jia-ke
authors.initials: JK
authors.lastname: Chai
authors.forename: Hui-feng
authors.initials: HF
authors.lastname: Song
authors.forename: Ming-huo
authors.initials: MH
authors.lastname: Xu
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D001696:Biomechanical Phenomena; D006820:Hyaluronic Acid / Q000494:pharmacology*; D011817:Rabbits; D016038:Skin Transplantation; D013552:Swine; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous
chemical_list: D006820:Hyaluronic Acid