title: [New approaches to therapy of persistent infections: elimination of intracellular Chlamydai trachomatis by exposure to low temperature argon plasma].
abstract: OBJECTIVE
  Study microbicidal activity of low temperature argon plasma (LTP) that is a stream of partially ionized argon having macroscopic temperature of the environment against Chlamydia trachomatis obligate intracellular parasites. Study viability of host cells in parallel.
  
  
  METHODS
  McCoy line cells infected with C. trachomatis (Bu-434/L2 strain) were exposed to LTP obtained by using atmospheric pressure plasma SHF generator. Intracellular localization of chlamydiae was visualized by luminescent microscopy.
  
  
  RESULTS
  Exposure of infected McCoy line cells resulted in the destruction of chlamydia inclusions and practically complete elimination of intracellular bacteria. At the same time LTP exposure did not result in immediate death of host cells, an insignificant reduction of the number of cells was observed 24 hours after the exposure to LTP.
  
  
  CONCLUSIONS
  The effect of LTP for elimination of intracellular chlamydia without significant changes in viability of eukaryotic host cells was demonstrated.
authors.forename: S A
authors.initials: SA
authors.lastname: Ermolaeva
authors.forename: E V
authors.initials: EV
authors.lastname: Sysoliatina
authors.forename: N I
authors.initials: NI
authors.lastname: Kolkova
authors.forename: M A
authors.initials: MA
authors.lastname: Drobiashchenko
authors.forename: M M
authors.initials: MM
authors.lastname: Vasil'ev
authors.forename: A I
authors.initials: AI
authors.lastname: Tukhvatulin
authors.forename: O F
authors.initials: OF
authors.lastname: Petrov
authors.forename: B S
authors.initials: BS
authors.lastname: Naroditskiĭ
authors.forename: G E
authors.initials: GE
authors.lastname: Morfill
authors.forename: V F
authors.initials: VF
authors.lastname: Fortov
authors.forename: A I
authors.initials: AI
authors.lastname: Grigor'ev
authors.forename: N A
authors.initials: NA
authors.lastname: Zigangirova
authors.forename: A L
authors.initials: AL
authors.lastname: Gintsburg
mesh_terms: D001128:Argon / Q000737:chemistry; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival; D002690:Chlamydia Infections / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000254:growth & development*; D006801:Humans; D050296:Microbial Viability; D008856:Microscopy, Fluorescence; D058626:Plasma Gases; D013045:Species Specificity; D013696:Temperature
chemical_list: D058626:Plasma Gases; D001128:Argon