title: In vitro antiviral activity of sulfated Auricularia auricula polysaccharides.
abstract: Total Auricularia auricula polysaccharide (AAP(t)) was prepared by extracting and removing the proteins. Column chromatography was used to further graded it into AAP(1) and AAP(2). Three AAPs were modified by chlorosulfonic acid-pyridine method to obtain three sulfated AAPs (sAAPs), sAAP(t), sAAP(1) and sAAP(2), respectively. Three sAAPs and Newcastle disease virus (NDV) were added into cultivation system of chicken embryo fibroblast (CEF) in three manners, pre-, post- and simultaneous-adding polysaccharide with NDV respectively, taking three non-modified AAPs as control. Their anti-viral activities were compared by MTT method. The results showed that sAAPs and AAPs at a certain concentration could significantly inhibit the cellular infectivity of NDV in three manners. The effects of sAAPs were better than that of AAPs. It indicated that sulfated modification could enhance the antiviral activity of AAP. sAAP(1) and sAAP(t) possessed stronger activity and would be as the component of a new-type antiviral drug.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: The Luong
authors.initials: TL
authors.lastname: Nguyen
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Fan
authors.forename: Junmin
authors.initials: J
authors.lastname: Wang
authors.forename: Saifuding
authors.initials: S
authors.lastname: Abula
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Qin
authors.forename: Xingying
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Chen
authors.forename: Shem Kakai
authors.initials: SK
authors.lastname: Khakame
authors.forename: Bao Khanh
authors.initials: BK
authors.lastname: Dang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000473:pathology / Q000821:virology*; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D009521:Newcastle Disease / Q000188:drug therapy* / Q000473:pathology / Q000821:virology; D009522:Newcastle disease virus
chemical_list: D000998:Antiviral Agents; D062610:Fungal Polysaccharides