title: Tumor chemosensitivity is correlated with expression of multidrug resistance associated factors in variously differentiated gastric carcinoma tissues.
abstract: OBJECTIVE
  To evaluate the relationship between chemosensitivities in vitro and expressions of multidrug resistance (MDR) associated factors in differentiated gastric carcinomas.
  
  
  METHODS
  Gastric carcinomas tissues of varying degree of differentiation (65 cases) were collected and chemosensitivities to 5-FU, HCPT, PTX, L-OHP, CDDP and eADM were detected by sulphorhodamine B (SRB) assay, and expressions of P-gp, GST-π, Topo IIα, p53, Bcl-2, Bax and Survivin were tested by immunohistochemical staining.
  
  
  RESULTS
  Inhibition rates of 5-FU, L-OHP and CDDP for well differentiated tumors were lower than those of poorly differentiated tumors (p<0.05). Expressions of P-gp, Bcl-2 and Survivin were higher in well differentiated than in poorly differentiated carcinomas (p<0.05); while expression of Topo IIα in well differentiated carcinomas was lower than in poorly differentiated carcinomas (p<0.05). The expression of MDR factors was different between well and poorly differentiated carcinomas.
  
  
  CONCLUSIONS
  Different MDR characteristics were exhibited in well and poorly differentiated gastric carcinomas, which may be caused by different expressed MDR associated factors in these tissues.
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, China. li_yong_hbth@126.com
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Bi-bo
authors.initials: BB
authors.lastname: Tan
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhao
authors.forename: Li-Qiao
authors.initials: LQ
authors.lastname: Fan
authors.forename: Yü
authors.initials: Y
authors.lastname: Liu
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Hao
authors.forename: Xüe-Feng
authors.initials: XF
authors.lastname: Zhao
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019008:Drug Resistance, Neoplasm; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051034:Inhibitor of Apoptosis Proteins / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D013274:Stomach Neoplasms / Q000737:chemistry / Q000188:drug therapy* / Q000473:pathology; D000077022:Survivin
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D000077022:Survivin