title: Consecutive targetable smart nanoprobe for molecular recognition of cytoplasmic microRNA in metastatic breast cancer.
abstract: We report smart nanoprobe, hyaluronic acid (HA)-based nanocontainers containing miR-34a beacons (bHNCs), for the intracellular recognition of miR-34a levels in metastatic breast cancer cells, which is distinct from the imaging of biomarkers such of cell membrane receptors such as HER2. In this study, we demonstrate that a nanoscale vesicle that couples a targeting endocytic route, CD44, and a molecular imaging probe enables the efficient detection of specific miRNAs. Furthermore, bHNCs showed no cytotoxicity and high stability due to the anchored HA molecules on the surface of nanocontainers, and enables the targeted delivery of beacons via CD44 receptor-mediated endocytosis. In vitro and in vivo optical imaging using bHNCs also allow the measurement of miR-34a expression levels due to the selective recognition of the beacons released from the internalized bHNCs. We believe that the technique described herein can be further developed as a cancer diagnostic as well as a miRNA-based therapy of metastatic cancer.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Oral Cancer Research Institute, College of Dentistry, Yonsei University, Seoul, Republic of Korea.
authors.forename: Eunjung
authors.initials: E
authors.lastname: Kim
authors.forename: Jaemoon
authors.initials: J
authors.lastname: Yang
authors.forename: Joseph
authors.initials: J
authors.lastname: Park
authors.forename: Soonhag
authors.initials: S
authors.lastname: Kim
authors.forename: Nam Hee
authors.initials: NH
authors.lastname: Kim
authors.forename: Jong In
authors.initials: JI
authors.lastname: Yook
authors.forename: Jin-Suck
authors.initials: JS
authors.lastname: Suh
authors.forename: Seungjoo
authors.initials: S
authors.lastname: Haam
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Huh
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000556:secondary*; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics*; D008856:Microscopy, Fluorescence / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry
chemical_list: D035683:MicroRNAs; D053769:Nanocapsules