title: Mouse oocyte meiosis is disturbed by knockdown of Suv4-20h.
abstract: Suv4-20h was initially characterised as a histone methyltransferase (HMTase) that catalyses lysine 20 of histone H4 dimethylation (H4K20me2) and trimethylation (H4K20me3). In the present study, using RNA interference (RNAi), we found that Suv4-20h activity is required for the fidelity of chromosome distribution during meiosis in the mammalian oocyte. Knockdown of Suv4-20h resulted in attenuation of H4K20me3 and the accumulation of H4K20me1. After Suv4-20h knockdown, oocytes exhibited an increasing percentage of aberrant chromosome alignment in MI, together with a decreasing percentage of polar body I extrusion. We conclude that Suv4-20h may be required for normal chromosome behaviour and that it is crucial for proper meiotic progression in mammalian oocytes.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xiong
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Xuguang
authors.initials: X
authors.lastname: Wang
authors.forename: Xueshan
authors.initials: X
authors.lastname: Ma
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Honglin
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D002503:Centromere / Q000378:metabolism; D044127:Epigenesis, Genetic; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006570:Heterochromatin / Q000378:metabolism; D011495:Histone-Lysine N-Methyltransferase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008540:Meiosis; D008677:Metaphase; D008745:Methylation; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D009866:Oogenesis; D059705:Polar Bodies / Q000166:cytology / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D006570:Heterochromatin; D006657:Histones; D007527:Isoenzymes; D012333:RNA, Messenger; C570043:Suv4-20h protein, mouse; D011495:Histone-Lysine N-Methyltransferase; D008239:Lysine