title: Intrauterine growth restriction modifies the normal gene expression in kidney from rabbit fetuses.
abstract: The aim of this work was to study the effect of intrauterine growth restriction (IUGR) on fetal kidneys. The IUGR was induced by uteroplacental vessels ligature in a model of pregnant rabbit. We centralized the study in the gene expression of essential proteins for fetal kidney development and kidney protection against hypoxia, osmotic stress, and kidney injury. The gene expression of HIF-1α, NFAT5, IL-1β, NGAL, and ATM were studied by qRT-PCR and Western blot in kidneys from control and IUGR fetuses. Experimental IUGR fetuses were significantly smaller than the control animals (39 vs. 48 g, p<0.05). The number of glomeruli was decreased in IUGR kidneys, without morphological alterations. IUGR increased the gene expression of HIF-1α, NFAT5, IL-1β, NGAL, and ATM (p<0.05) in kidneys of fetuses undergoing IUGR, suggesting that fetal blood flow restriction produce alterations in gene expression in fetal kidneys.
authors.affiliation: Department of Obstetrics & Gynecology, Faculty of Medicine, Universidad de los Andes, Santiago, Chile.
authors.forename: Horacio
authors.initials: H
authors.lastname: Figueroa
authors.forename: Mauricio
authors.initials: M
authors.lastname: Lozano
authors.forename: Cristian
authors.initials: C
authors.lastname: Suazo
authors.forename: Elisenda
authors.initials: E
authors.lastname: Eixarch
authors.forename: Sebastian E
authors.initials: SE
authors.lastname: Illanes
authors.forename: Juan Eduardo
authors.initials: JE
authors.lastname: Carreño
authors.forename: Sandra
authors.initials: S
authors.lastname: Villanueva
authors.forename: Edgar
authors.initials: E
authors.lastname: Hernández-Andrade
authors.forename: Eduard
authors.initials: E
authors.lastname: Gratacós
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Irarrazabal
mesh_terms: D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics* / Q000378:metabolism; D005333:Fetus / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D020043:Genes, Essential; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000196:embryology* / Q000293:injuries / Q000378:metabolism; D054834:Lipocalins / Q000235:genetics / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000098:blood supply; D011247:Pregnancy; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D053583:Interleukin-1beta; D054834:Lipocalins; D050778:NFATC Transcription Factors; D025521:Tumor Suppressor Proteins; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases