title: An RNA splicing enhancer that does not act by looping.
abstract: Out of the loop: Do the proteins bound to an enhancer site on pre-mRNA interact directly with the splice site by diffusion (looping), as is generally accepted, or does the intervening RNA play a role? By inserting a PEG linker between an enhancer sequence and alternative splice sites, the interaction of these two elements can be studied. Intervening RNA was essential for the enhancer activity, which rules out the looping model.
authors.affiliation: Department of Chemistry, University of Leicester, Leicester, UK.
authors.forename: Helen
authors.initials: H
authors.lastname: Lewis
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Perrett
authors.forename: Glenn A
authors.initials: GA
authors.lastname: Burley
authors.forename: Ian C
authors.initials: IC
authors.lastname: Eperon
mesh_terms: D000818:Animals; D001483:Base Sequence; D057930:Click Chemistry; D004742:Enhancer Elements, Genetic; D006801:Humans; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D022821:RNA Splice Sites; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D009841:Oligonucleotides; D012322:RNA Precursors; D022821:RNA Splice Sites; D012333:RNA, Messenger