title: MicroRNA-125b functions as a tumor suppressor in hepatocellular carcinoma cells.
abstract: MicroRNAs (miRNAs) are important regulators of multiple cellular processes, and the deregulation of miRNA is a common event in diverse human diseases, particularly cancer. However, the mechanisms underlying the relationship between disordered miRNA expression and tumorigenesis have remained largely unknown. In this study, we demonstrated the down-regulation of miR-125b in hepatocellular carcinoma (HCC) tissues and HCC cell lines by Northern blot and quantitative RT-PCR analyses. The ectopic expression of miR-125b reduced the cellular proliferation and cell cycle progression of HCC cells by targeting Mcl-1 and IL6R. Furthermore, the miR-125b-induced inhibition of cell proliferation was rescued by the expression of Mcl-1 or IL6R variants that lacked 3' UTRs. Thus, this study revealed the differential expression of miR-125b in HCC cells and elucidated its potential as a tumor suppressor in HCC development.
authors.affiliation: Department of Surgery, The First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Jia
authors.affiliation: Department of Thoracic Surgery, Shanxi Cancer Hospital, Taiyuan 030001, China.
authors.forename: Yu-Xuan
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology, Beijing 100005, China.
authors.forename: Wen-Ting
authors.initials: WT
authors.lastname: Yan
authors.affiliation: Department of Surgery, The First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Hui-Yu
authors.initials: HY
authors.lastname: Li
authors.affiliation: Department of Surgery, The First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Yan-Zhang
authors.initials: YZ
authors.lastname: Tian
authors.affiliation: Department of Surgery, The First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Shi-Ming
authors.initials: SM
authors.lastname: Wang
authors.affiliation: Department of Surgery, The First Hospital of Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Hao-Liang
authors.initials: HL
authors.lastname: Zhao
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D019947:Receptors, Interleukin-6 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C508603:IL6R protein, human; C579095:MCL1 protein, human; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D012334:RNA, Neoplasm; D019947:Receptors, Interleukin-6