title: Toxicity study and evaluation of biochemical markers towards the identification of the causative agent for an outbreak of liver disease in Tahtay Koraro Woreda, Tigray.
abstract: BACKGROUND
  A team of experts of the Faculty of Medicine, Addis Ababa University reported the emergence of unidentified fatal liver disease in Tahtay Koraro Woreda, Tigray in the mid of December 2005. The EHNRI has been then instructed to investigate the possible etiological agent that are likely to be responsible in triggering the health problem and a field survey team consisting of experts were went to the affected area to investigate the situations surrounding the disease.
  
  
  OBJECTIVE
  This investigation was conducted to determine the possible etiological agent(s) for the stated health problem in the affected village.
  
  
  METHODS
  Acute toxicity study was performed on animal model for the various samples used in human consumption, which was followed by histopathological examination of the liver of the sacrificed laboratory animals. In order to facilitate the elucidation of the causative agent for the alleged health problem further tests for clinical markers and antigens were also performed on the serum collected from affected persons.
  
  
  RESULTS
  Neither death nor toxic symptoms manifestations were observed on laboratory animals when feeding the consumable samples for a period of two weeks, however histopathological examination of the liver of the sacrificed animals that were given the unprotected pond water and Tela samples from the affected village as a drink revealed severe hepatoic necrosis. Biochemical test results of the serum samples revealed raised level of some clinical markers that are highly significant for detecting liver abnormality of toxic origin. Serological test for surface antigen ruled out the possible causes of infectious origin such as viral hepatitis.
  
  
  CONCLUSIONS
  The overall results confirmed that the causative agent for the outbreak of the liver disease was of toxic origin rather than due to infectious agent and this was found to be associated with consumption of contaminated water as well as Tela.
authors.affiliation: Ethiopian Health and Nutrition Research Institute, Addis Ababa, Ethiopia, P. O. Box 1242, Addis Ababa.
authors.forename: Asfaw
authors.initials: A
authors.lastname: Debella
authors.forename: Dawit
authors.initials: D
authors.lastname: Abebe
authors.forename: Frew
authors.initials: F
authors.lastname: Tekabe
authors.forename: Hailu
authors.initials: H
authors.lastname: Mamo
authors.forename: Almaz
authors.initials: A
authors.lastname: Abebe
authors.forename: Bekure
authors.initials: B
authors.lastname: Tsegaye
authors.forename: Gonfa
authors.initials: G
authors.lastname: Ayana
authors.forename: Ambaye
authors.initials: A
authors.lastname: Degefa
authors.forename: Paulos
authors.initials: P
authors.lastname: Negussie
authors.forename: Eshetu
authors.initials: E
authors.lastname: Yimer
authors.forename: Feyessa
authors.initials: F
authors.lastname: Challa
authors.forename: Eshetu
authors.initials: E
authors.lastname: Lemma
authors.forename: Alemtsehaye
authors.initials: A
authors.lastname: Tefera
authors.forename: Yared
authors.initials: Y
authors.lastname: Mekonnen
authors.forename: Negede
authors.initials: N
authors.lastname: Afework
authors.forename: Kissie
authors.initials: K
authors.lastname: Mudie
authors.forename: Ashinef
authors.initials: A
authors.lastname: Tadele
authors.forename: Tsegaye
authors.initials: T
authors.lastname: Kidanemariam
authors.forename: Berhanu
authors.initials: B
authors.lastname: Muchie
authors.forename: Negussie
authors.initials: N
authors.lastname: Dadi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004196:Disease Outbreaks / Q000706:statistics & numerical data*; D005002:Ethiopia / Q000453:epidemiology; D005260:Female; D006801:Humans; D008107:Liver Diseases / Q000453:epidemiology* / Q000209:etiology*; D008297:Male; D023421:Models, Animal; D014876:Water Pollution / Q000009:adverse effects*
chemical_list: D015415:Biomarkers