title: Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies.
abstract: Plasmids containing a site-specific DNA interstrand cross-link (ICL) are invaluable tools for the investigation of ICL repair pathways at the biochemical and cellular level. We describe a procedure for preparation of plasmid DNA substrates containing a single ICL at a specific site. The procedure is versatile, leads to reliable yields of pure DNA substrate, and is suitable for the incorporation of virtually any type of DNA lesion into plasmids.
authors.affiliation: Institute of Molecular Cancer Research, University of Zürich, Zürich, Switzerland.
authors.forename: Milica
authors.initials: M
authors.lastname: Enoiu
authors.forename: The Vinh
authors.initials: TV
authors.lastname: Ho
authors.forename: David T
authors.initials: DT
authors.lastname: Long
authors.forename: Johannes C
authors.initials: JC
authors.lastname: Walter
authors.forename: Orlando D
authors.initials: OD
authors.lastname: Schärer
mesh_terms: D001483:Base Sequence; D002945:Cisplatin / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D018736:DNA Adducts / Q000378:metabolism; D004260:DNA Repair / Q000187:drug effects; D004262:DNA Restriction Enzymes / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D018736:DNA Adducts; D004247:DNA; D004262:DNA Restriction Enzymes; D002945:Cisplatin