title: Molecular characterization of Giardia psittaci by multilocus sequence analysis.
abstract: Multilocus sequence analyses targeting small subunit ribosomal DNA (SSU rDNA), elongation factor 1 alpha (ef1α), glutamate dehydrogenase (gdh), and beta giardin (β-giardin) were performed on Giardia psittaci isolates from three Budgerigars (Melopsittacus undulates) and four Barred parakeets (Bolborhynchus lineola) kept in individual households or imported from overseas. Nucleotide differences and phylogenetic analyses at four loci indicate the distinction of G. psittaci from the other known Giardia species: Giardia muris, Giardia microti, Giardia ardeae, and Giardia duodenalis assemblages. Furthermore, G. psittaci was related more closely to G. duodenalis than to the other known Giardia species, except for G. microti. Conflicting signals regarded as "double peaks" were found at the same nucleotide positions of the ef1α in all isolates. However, the sequences of the other three loci, including gdh and β-giardin, which are known to be highly variable, from all isolates were also mutually identical at every locus. They showed no double peaks. These results suggest that double peaks found in the ef1α sequences are caused not by mixed infection with genetically different G. psittaci isolates but by allelic sequence heterogeneity (ASH), which is observed in diplomonad lineages including G. duodenalis. No sequence difference was found in any G. psittaci isolates at the gdh and β-giardin, suggesting that G. psittaci is indeed not more diverse genetically than other Giardia species. This report is the first to provide evidence related to the genetic characteristics of G. psittaci obtained using multilocus sequence analysis.
authors.affiliation: Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan. ni-abe@city.osaka.lg.jp
authors.forename: Niichiro
authors.initials: N
authors.lastname: Abe
authors.forename: Ikuko
authors.initials: I
authors.lastname: Makino
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kojima
mesh_terms: D000818:Animals; D001483:Base Sequence; D016000:Cluster Analysis; D003598:Cytoskeletal Proteins / Q000235:genetics; D004247:DNA / Q000032:analysis / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005872:Giardia / Q000145:classification* / Q000235:genetics*; D005873:Giardiasis / Q000469:parasitology* / Q000662:veterinary*; D005969:Glutamate Dehydrogenase / Q000235:genetics; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D010240:Parakeets / Q000469:parasitology; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D015800:Protozoan Proteins / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D003598:Cytoskeletal Proteins; D020648:Peptide Elongation Factor 1; D015800:Protozoan Proteins; C038207:giardin protein, Giardia lamblia; D004247:DNA; D005969:Glutamate Dehydrogenase