title: Reed canary grass as a feedstock for 2nd generation bioethanol production.
abstract: The enzymatic hydrolysis and fermentation of reed canary grass, harvested in the spring or autumn, and barley straw were studied. Steam pretreated materials were efficiently hydrolysed by commercial enzymes with a dosage of 10-20FPU/g d.m. Reed canary grass harvested in the spring was hydrolysed more efficiently than the autumn-harvested reed canary grass. Additional β-glucosidase improved the release of glucose and xylose during the hydrolysis reaction. The hydrolysis rate and level of reed canary grass with a commercial Trichoderma reesei cellulase could be improved by supplementation of purified enzymes. The addition of CBH II improved the hydrolysis level by 10% in 48hours' hydrolysis. Efficient mixing was shown to be important for hydrolysis already at 10% dry matter consistency. The highest ethanol concentration (20g/l) and yield (82%) was obtained with reed canary grass at 10% d.m. consistency.
authors.affiliation: VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Finland. anne.kallioinen@vtt.fi
authors.forename: Anne
authors.initials: A
authors.lastname: Kallioinen
authors.forename: Jaana
authors.initials: J
authors.lastname: Uusitalo
authors.forename: Katri
authors.initials: K
authors.lastname: Pahkala
authors.forename: Markku
authors.initials: M
authors.lastname: Kontturi
authors.forename: Liisa
authors.initials: L
authors.lastname: Viikari
authors.forename: Niklas von
authors.initials: Nv
authors.lastname: Weymarn
authors.forename: Matti
authors.initials: M
authors.lastname: Siika-Aho
mesh_terms: D056804:Biofuels / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D031783:Phalaris / Q000378:metabolism*; D013227:Steam; D014994:Xylose / Q000378:metabolism
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D013227:Steam; D000431:Ethanol; D014994:Xylose; D005947:Glucose