title: Human embryonic stem cell-derived mesodermal progenitors display substantially increased tissue formation compared to human mesenchymal stem cells under dynamic culture conditions in a packed bed/column bioreactor.
abstract: Bone tissue engineering represents a promising strategy to obviate bone deficiencies, allowing the ex vivo construction of bone substitutes with unprecedented potential in the clinical practice. Considering that in the human body cells are constantly stimulated by chemical and mechanical stimuli, the use of bioreactor is emerging as an essential factor for providing the proper environment for the reproducible and large-scale production of the engineered substitutes. Human mesenchymal stem cells (hMSCs) are experimentally relevant cells but, regardless the encouraging results reported after culture under dynamic conditions in bioreactors, show important limitations for tissue engineering applications, especially considering their limited proliferative potential, loss of functionality following protracted expansion, and decline in cellular fitness associated with aging. On the other hand, we previously demonstrated that human embryonic stem cell-derived mesodermal progenitors (hES-MPs) hold great potential to provide a homogenous and unlimited source of cells for bone engineering applications. Based on prior scientific evidence using different types of stem cells, in the present study we hypothesized that dynamic culture of hES-MPs in a packed bed/column bioreactor had the potential to affect proliferation, expression of genes involved in osteogenic differentiation, and matrix mineralization, therefore resulting in increased bone-like tissue formation. The reported findings suggest that hES-MPs constitute a suitable alternative cell source to hMSCs and hold great potential for the construction of bone substitutes for tissue engineering applications in clinical settings.
authors.affiliation: The New York Stem Cell Foundation, New York, New York, USA. gdepeppo@nyscf.org
authors.forename: Giuseppe Maria
authors.initials: GM
authors.lastname: de Peppo
authors.forename: Martina
authors.initials: M
authors.lastname: Sladkova
authors.forename: Peter
authors.initials: P
authors.lastname: Sjövall
authors.forename: Anders
authors.initials: A
authors.lastname: Palmquist
authors.forename: Karim
authors.initials: K
authors.lastname: Oudina
authors.forename: Johan
authors.initials: J
authors.lastname: Hyllner
authors.forename: Peter
authors.initials: P
authors.lastname: Thomsen
authors.forename: Hervé
authors.initials: H
authors.lastname: Petite
authors.forename: Camilla
authors.initials: C
authors.lastname: Karlsson
mesh_terms: D061249:Batch Cell Culture Techniques / Q000295:instrumentation*; D019149:Bioreactors; D001846:Bone Development / Q000502:physiology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D040542:Mechanotransduction, Cellular / Q000502:physiology; D059630:Mesenchymal Stem Cells; D008648:Mesoderm / Q000166:cytology*; D009924:Organ Culture Techniques / Q000295:instrumentation*; D010012:Osteogenesis / Q000502:physiology*