title: A cancer/testis antigen microarray to screen autoantibody biomarkers of non-small cell lung cancer.
abstract: Cancer/testis antigens (CTAs) are highly immunogenic in many tumors, especially in non-small cell lung cancer (NSCLC). A low-density protein microarray, which consisted of 72 CTAs and six non-CTAs, was used to screen for lung cancer-related autoantibodies. The CTA panel of NY-ESO-1, XAGE-1, ADAM29 and MAGEC1, had sensitivity and specificity values of 33% and 96%, respectively. When examined in a test set, this panel of markers had sensitivity and specificity values of 36% and 89%, respectively. This array of markers preferentially detected NSCLC, but did not detect breast cancer, and non-cancer lung disease.
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101318, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Shan
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Lou
authors.forename: Ting
authors.initials: T
authors.lastname: Xiao
authors.forename: Ju
authors.initials: J
authors.lastname: Zhang
authors.forename: Huiying
authors.initials: H
authors.lastname: Sun
authors.forename: Yanning
authors.initials: Y
authors.lastname: Gao
authors.forename: Shujun
authors.initials: S
authors.lastname: Cheng
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.forename: Ningzhi
authors.initials: N
authors.lastname: Xu
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
mesh_terms: D000368:Aged; D000951:Antigens, Neoplasm / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000276:immunology*; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000276:immunology*; D008297:Male; D046228:Microarray Analysis / Q000379:methods*; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D000951:Antigens, Neoplasm; D001323:Autoantibodies; D014408:Biomarkers, Tumor