title: [Culture and induced multilineage differentiation of mesenchymal stem cells derived from human nasal mucosa].
abstract: OBJECTIVE
  To establish an in vitro method to culture mesenchymal stem cells(MSCs) derived from human nasal mucosa, and explore their stemness and differentiation potential.
  
  
  METHODS
  Based on the observation of distribution of MSCs in human nasal mucosa, we cultured and proliferated MSCs in vitro and identified the expression of stem cell markers on them including Nestin, CD133, Vimentin and Sa114 with immunofluorescence. The MSCs were induced to differentiate to osteoblasts with medium containing dexamethasone, ascorbic acid and beta sodium glycerophosphate, and to neurons with Neurobasal medium containing B27, ATRA and TSA. Histochemistry and immunofluorescence were applied to evaluate the differentiation.
  
  
  RESULTS
  The nestin and vimentin immunofluorescence-positive MSCs existed extensively in human nasal mucosa. While the MSCs were cultured in the osteogenic-inducing medium, activities of alkaline phosphatase were increased significantly, and bone nodules were found on the surface of the osteoblasts by alizarin red staining. After the induction by neural-inducing medium, the MSCs adopted neuron like appearance with many slim protrusions interconnected as a network. The induced cells expressed neural markers NF-200 and BM88 strongly.
  
  
  CONCLUSIONS
  The MSCs derived from human nasal mucosa are multipotent stem cells and can be utilized as seed cells to repair bone or neural injury.
authors.affiliation: Department of Otolaryngology, the Affiliated Hospital of Jiangsu University, Zhenjiang, 212002, China.
authors.forename: Qiusheng
authors.initials: Q
authors.lastname: Huang
authors.forename: Hanqiang
authors.initials: H
authors.lastname: Lu
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Zhou
authors.forename: Qinghua
authors.initials: Q
authors.lastname: He
authors.forename: Xianglan
authors.initials: X
authors.lastname: Sun
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells; D009297:Nasal Mucosa / Q000166:cytology*; D009474:Neurons; D010006:Osteoblasts / Q000166:cytology*
chemical_list: D000469:Alkaline Phosphatase