title: Formation of a heterooctameric complex between aspartate α-decarboxylase and its cognate activating factor, PanZ, is CoA-dependent.
abstract: The existence of a fifth essential protein for pantothenate biosynthesis in some enteric bacteria has recently been reported by Stuecker et al. [10] and Nozaki et al. (in press) [9]. This protein, PanZ, catalyses the activation of the PanD zymogen to form ADC and is essential for prototrophic growth. In this paper, we characterise the interaction of PanZ with coenzyme A and a constitutively inactive mutant of PanD using a combination of isothermal titration calorimetry and mass spectrometry. These approaches reveal that the two proteins interact with nanomolar affinity in a CoA-dependent fashion to form a heterooctameric complex.
authors.affiliation: School of Chemistry, University of Leeds, Leeds LS2 9JT, UK.
authors.forename: Diana C F
authors.initials: DC
authors.lastname: Monteiro
authors.forename: Michael D
authors.initials: MD
authors.lastname: Rugen
authors.forename: Dale
authors.initials: D
authors.lastname: Shepherd
authors.forename: Shingo
authors.initials: S
authors.lastname: Nozaki
authors.forename: Hironori
authors.initials: H
authors.lastname: Niki
authors.forename: Michael E
authors.initials: ME
authors.lastname: Webb
mesh_terms: D002151:Calorimetry; D003065:Coenzyme A / Q000737:chemistry*; D004792:Enzyme Precursors / Q000737:chemistry*; D005968:Glutamate Decarboxylase / Q000737:chemistry*; D055503:Protein Multimerization
chemical_list: D004792:Enzyme Precursors; C019104:aspartate-alpha-decarboxylase; D005968:Glutamate Decarboxylase; D003065:Coenzyme A