title: A beta-defensin 1-like antimicrobial peptide from the tree shrew, Tupaia belangeri.
abstract: A novel beta-defensin 1-like antimicrobial peptide (β-defensin 1TB) containing 36 amino acid residues was purified and characterized from the serum of the tree shrew, Tupaia belangeri. Its amino acid sequence was determined as DHYLCVKNEGICLYSSCPSYTKIEGTCYGGKAKCCK, by Edman degradation, mass spectrometry analysis, and cDNA cloning. Evolution analysis indicated that β-defensin 1TB showed maximal similarity to the β-defensin 1 identified from cotton-top tamarin, Saguinus oedipus. β-defensin 1TB exerted potential antimicrobial activities against wide spectrum of microorganisms including Gram-negative and -positive bacteria and fungi. It showed little hemolitic activity to human or rabbit red cells. To the best of our knowledge, this is the first report of antimicrobial peptide from Tupaiidae.
authors.affiliation: Life Sciences College of Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Dong
authors.forename: Longbao
authors.initials: L
authors.lastname: Lv
authors.forename: Xiuwen
authors.initials: X
authors.lastname: Yan
authors.forename: Ren
authors.initials: R
authors.lastname: Lai
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004912:Erythrocytes / Q000187:drug effects; D005658:Fungi / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D010802:Phylogeny; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014417:Tupaia / Q000097:blood*; D023083:beta-Defensins / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D018076:DNA, Complementary; D010446:Peptide Fragments; D023083:beta-Defensins