title: Immunological mechanisms involved in the protection against intestinal taeniosis elicited by oral immunization with Taenia solium calreticulin.
abstract: Oral immunization with functional recombinant Taenia solium calreticulin (rTsCRT) induces 37% reduction in tapeworm burden in the experimental model of intestinal taeniosis in hamsters. Furthermore, tapeworms recovered from vaccinated animals exhibit diminished length, being frequently found in more posterior parts of the small intestine. The aim of this study was to analyze the immunological mechanisms involved in protection in response to rTsCRT oral immunization. Hamsters were orally immunized with rTsCRT using cholera toxin (CT) as adjuvant, weekly for 4 weeks. Fifteen days after the last boost animals were challenged with four T. solium cysticerci. Reduction in the adult worm recovery and increased transcription of mRNA for IL-4 and IFN-γ in the mucosa of rTsCRT+CT immunized animals were observed. Immunization also induced goblet cell hyperplasia in the mucosa surrounding the implantation site of the parasite. Specific IgG and IgA antibodies in serum and fecal supernatants were detected after the second immunization, being more pronounced after challenge. Our data suggest that oral vaccination with rTsCRT+CT regulates a local expression of IL-4 and IFN-γ, stimulating secretion of IgA that, together with the increase of goblet cells and mucin production, could result in an unfavorable environment for T. solium promoting an impaired tapeworm development.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Av. Universidad 3000, Colonia Copilco-Universidad, DF 04510, México, Mexico.
authors.forename: Sonia
authors.initials: S
authors.lastname: Leon-Cabrera
authors.forename: Mayra
authors.initials: M
authors.lastname: Cruz-Rivera
authors.forename: Fela
authors.initials: F
authors.lastname: Mendlovic
authors.forename: Mirza
authors.initials: M
authors.lastname: Romero-Valdovinos
authors.forename: Gilberto
authors.initials: G
authors.lastname: Vaughan
authors.forename: Ana María
authors.initials: AM
authors.lastname: Salazar
authors.forename: Guillermina
authors.initials: G
authors.lastname: Avila
authors.forename: Ana
authors.initials: A
authors.lastname: Flisser
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis / Q000097:blood; D037282:Calreticulin / Q000008:administration & dosage / Q000276:immunology*; D006224:Cricetinae; D005243:Feces / Q000737:chemistry; D005260:Female; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D008647:Mesocricetus; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000276:immunology; D013552:Swine; D041201:Taenia solium / Q000737:chemistry / Q000276:immunology*; D013622:Taeniasis / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination / Q000379:methods*
chemical_list: D000909:Antibodies, Helminth; D037282:Calreticulin; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011994:Recombinant Proteins