title: Dendritic cells with CED-3 and CED-4 siRNA is effective in prolonging DC lifetime and suppresses tumor growth in gastric cancer.
abstract: OBJECTIVE
  We investigated effects of CED-3 and CED-4-siRNA on prolonging dendritic cell life in vivo and in vitro.
  
  
  METHODS
  The DCs were divided into three groups: pure-DC, siRNA and CED-3 and CED-4-siRNA. we performed anti-apoptosis assays for DCs with flow cytometry. The assay for cytotoxicity assay was performed in vitro by a standard chromium assay at various effector/target ratios. Percent-specific lysis was calculated. We injected three kinds of DCs from tail vein every 3 days, we calculated tumor volume control rate and tumor weight control rate with formula.
  
  
  RESULTS
  The DC percentages of apoptosis of CED-3 and CED-4 siRNA group were (12.09±1.14)%. Tumor-specific CTL activity showed 82.1% specific lysis for CED-3 and CED-4-siRNA DC group and 39.4% and 40.2% specific lysis for pure DC and siRNA DC group respectively. The lysis of CED-3 and CED-4-siRNA group was higher than the any other groups (p<0.05).The experiment of transplantation tumor in BALB/C mice showed that CED-3 and CED-4-siRNA DC can inhibit mice with tumors in volume and weight.
  
  
  CONCLUSIONS
  We found that vaccination with CED-3 and CED-4-siRNA was capable of prolonging the survival of antigen-expressing DCs, and generated a strong therapeutic effect in the treatment of gastric cancer.
authors.affiliation: Department of Gastrointestinal Surgery, Renmin Hospital of Wuhan University, Hubei Province, China. sunhuawenmail@gmail.com
authors.forename: Hua-Wen
authors.initials: HW
authors.lastname: Sun
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.forename: Hong-Xue
authors.initials: HX
authors.lastname: Wu
authors.forename: Chong
authors.initials: C
authors.lastname: Wu
mesh_terms: D000818:Animals; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000502:physiology; D020169:Caspases / Q000502:physiology*; D003713:Dendritic Cells / Q000276:immunology* / Q000502:physiology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D034741:RNA, Small Interfering / Q000235:genetics*; D013274:Stomach Neoplasms / Q000473:pathology / Q000628:therapy*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D051017:Apoptosis Regulatory Proteins; C485604:CASP8AP2 protein, human; D002135:Calcium-Binding Proteins; D034741:RNA, Small Interfering; D020169:Caspases