title: [Correlation between polymorphism of sex hormone binding globulin and occurrence of hepatocellular carcinoma].
abstract: OBJECTIVE
  This study aims to investigate the correlation between polymorphism of sex hormone-binding globulin (SHBG) Asp327Asn (rs6259) locus and occurrence of hepatocellular carcinoma (HCC).
  
  
  METHODS
  621 cases with HCC and 621 cancer-free controls from two hospitals of Guangxi were recruited from January, 2007 to June, 2010. Single nucleotide polymorphisms (SNP) of SHBG Asp327Asn were detected by ABI7500 Fast Real-Time fluorescence quantitative PCR. Multivariate unconditional logistic regression was applied to analyze risk of HCC among different genotypes carriers and their interaction with the exposure factors. The Kaplan-Meier survival analysis was used to detect the relationship between onset age of HCC and genotypes.
  
  
  RESULTS
  The frequencies of Asp/Asp, Asp/Asn and Asn/Asn genotype in case group were 86.31% (536/621), 12.40% (77/621) and 1.29% (8/621), respectively; while those in control group were 81.00% (503/621), 17.39% (108/621) and 1.61% (10/621), respectively. Significant difference in the genotype frequencies distribution was found between case and control groups (χ2=6.465, P<0.05). Compared with those harboring Asp/Asp genotype, multivariate logistic regression analysis revealed that the HCC risk of Asn/Asn+Asp/Asn genotype carriers was significantly decreased (adjusted OR=0.63, 95%CI: 0.40-0.98). Interaction analysis showed that there was interaction between the polymorphisms and two exposure factors, drinking (adjusted OR=3.45, 95%CI: 1.74-6.83) and HBV infection (adjusted OR=40.77, 95%CI: 21.60-76.97). Among those male patients with history of drinking, survival analysis indicated that the mean age of onset of individuals harboring Asp/Asp genotypes ((47.99±0.75) years-old) was 6 years earlier than those with Asn/Asn or Asp/Asn genotypes ((53.68±2.07) years-old) (χ2=6.91, P<0.01).
  
  
  CONCLUSIONS
  Polymorphism of SHBG (Asp327Asn) may be associated with both the risk of HCC occurrence and onset age of HCC.
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Guangxi Medical University, Nanning 530021, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Liu
authors.forename: Xiao-qiang
authors.initials: XQ
authors.lastname: Qiu
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Zeng
authors.forename: Hua
authors.initials: H
authors.lastname: Bai
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Dong-xiang
authors.initials: DX
authors.lastname: Pan
authors.forename: Liang-qin
authors.initials: LQ
authors.lastname: Mao
authors.forename: Qiu-lan
authors.initials: QL
authors.lastname: Huang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D008113:Liver Neoplasms / Q000453:epidemiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012738:Sex Hormone-Binding Globulin / Q000235:genetics*
chemical_list: D012738:Sex Hormone-Binding Globulin