title: Biosynthetic pathway for poly(3-hydroxypropionate) in recombinant Escherichia coli.
abstract: Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. In this study, we engineered a P3HP biosynthetic pathway in recombinant Escherichia coli. The genes for malonyl-CoA reductase (mcr, from Chloroflexus aurantiacus), propionyl-CoA synthetase (prpE, from E. coli), and polyhydroxyalkanoate synthase (phaC1, from Ralstonia eutropha) were cloned and expressed in E. coli. The E. coli genes accABCD encoding acetyl-CoA carboxylase were used to channel the carbon into the P3HP pathway. Using glucose as a sole carbon source, the cell yield and P3HP content were 1.32 g/L and 0.98% (wt/wt [cell dry weight]), respectively. Although the yield is relatively low, our study shows the feasibility of engineering a P3HP biosynthetic pathway using a structurally unrelated carbon source in bacteria.
authors.affiliation: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, P. R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Changshui
authors.initials: C
authors.lastname: Liu
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guang
authors.initials: G
authors.lastname: Zhao
mesh_terms: D001704:Biopolymers / Q000096:biosynthesis*; D053898:Biosynthetic Pathways / Q000235:genetics*; D002244:Carbon / Q000378:metabolism; D041861:Chloroflexus / Q000201:enzymology / Q000235:genetics; D003470:Culture Media / Q000737:chemistry; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis; D060847:Metabolic Engineering
chemical_list: D001704:Biopolymers; D003470:Culture Media; D019344:Lactic Acid; D002244:Carbon; C031601:hydracrylic acid; D005947:Glucose