title: [Unusual forms of persistence of Mycoplasma hominis in organism of infected humans].
abstract: OBJECTIVE
  Study previously unknown forms of persistence of Mycoplasma hominis in host organism.
  
  
  METHODS
  Culture method was used for detection of mycoplasmas. Identification was carried out by serological, electron microscopy methods, classic PCR and real time PCR; circulating immune complexes (CIC) were isolated by PEG precipitation.
  
  
  RESULTS
  Classic micoplasma cultures could not be isolated from blood even once. At the same time "mini-colony" cultures composed of mini-cells that were hardly passaged but sometimes formed continuous layer of the same colonies were isolated from blood serum samples with high frequency. During reseeding for more than 1 year they never acquired classic form. Not only antigens of M. hominis but its DNA were shown to be present in CIC. Viable cells forming "mini-colonies" identical to those isolated from blood sera were isolated from circulating immune complexes. A system of evidence on identity of isolated M. hominis cultures is presented. Cultures had infectivity and an ability to persist in organs of experimentally infected mice.
  
  
  CONCLUSIONS
  The isolated forms are apparently the result of adaptation of mycoplasmas to humoral immunity factors.
authors.forename: G A
authors.initials: GA
authors.lastname: Levina
authors.forename: O I
authors.initials: OI
authors.lastname: Barkhatova
authors.forename: L G
authors.initials: LG
authors.lastname: Gorina
authors.forename: N A
authors.initials: NA
authors.lastname: Gamova
authors.forename: S A
authors.initials: SA
authors.lastname: Goncharova
authors.lastname: Miller G G
authors.forename: T M
authors.initials: TM
authors.lastname: Raskova
authors.forename: I N
authors.initials: IN
authors.lastname: Rastegaeva
authors.forename: N A
authors.initials: NA
authors.lastname: Seliverstova
authors.forename: I V
authors.initials: IV
authors.lastname: Rakovskaia
mesh_terms: D000222:Adaptation, Physiological / Q000276:immunology; D000818:Animals; D000936:Antigen-Antibody Complex / Q000097:blood; D011232:Chemical Precipitation; D004269:DNA, Bacterial / Q000032:analysis*; D006801:Humans; D056724:Immunity, Humoral; D051379:Mice; D008854:Microscopy, Electron; D009175:Mycoplasma Infections / Q000097:blood* / Q000276:immunology / Q000382:microbiology; D019535:Mycoplasma hominis / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D011092:Polyethylene Glycols / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000936:Antigen-Antibody Complex; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011092:Polyethylene Glycols