title: Genetic module and miRNome trait analyses reflect the distinct biological features of endothelial progenitor cells from different anatomic locations.
abstract: BACKGROUND
  Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair, yet EPCs from different anatomic locations possess unique biological properties. The underlying mechanisms are unclear.
  
  
  RESULTS
  EPCs from CB expressed abundant genes involved in cell cycle, hypoxia signalling and blood vessel development, correlating with the phenotypes that CB-EPCs proliferated more rapidly, migrated faster, and formed tubule structure more efficiently. smRNA-seq further deciphered miRNome patterns in EPCs isolated from CB or PB: 54 miRNAs were enriched in CB-EPCs, while another 50 in PB-EPCs. Specifically, CB-EPCs expressed more angiogenic miRNAs such as miR-31, while PB-EPCs possessed more tumor suppressive miRNAs including miR-10a. Knocking down miR-31 levels in CB-EPCs suppressed cell migration and microtubule formation, while overexpressing miR-31 in PB-EPCs helped to recapitulate some of CB-EPC functions.
  
  
  CONCLUSIONS
  Our results show the foundation for a more detailed understanding of EPCs from different anatomic sources. Stimulating the expression of angiogenic microRNAs or genes in EPCs of low activity (such as those from patients with cardiovascular diseases) might allow the development of novel therapeutic strategies.
authors.affiliation: Division of Cardiology, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Cheng-Chung
authors.initials: CC
authors.lastname: Cheng
authors.forename: Hung-Hao
authors.initials: HH
authors.lastname: Lo
authors.forename: Tse-Shun
authors.initials: TS
authors.lastname: Huang
authors.forename: Yi-Chieh
authors.initials: YC
authors.lastname: Cheng
authors.forename: Shi-Ting
authors.initials: ST
authors.lastname: Chang
authors.forename: Shing-Jyh
authors.initials: SJ
authors.lastname: Chang
authors.forename: Hsei-Wei
authors.initials: HW
authors.lastname: Wang
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D019295:Computational Biology; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology; D020869:Gene Expression Profiling; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: C547183:MIRN10 microRNA, human; C534612:MIRN31 microRNA, human; D035683:MicroRNAs