title: Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts.
abstract: Interstrand cross-links (ICL) are one of the most hazardous types of DNA damage as they form a roadblock to all processes that involve strand separation. Repair of these lesions involves several different DNA repair pathways, but the molecular mechanism is unclear. Here we describe a system that allows the examination of ICL repair, via a physiological mechanism, in vitro. This system, which uses Xenopus egg extracts in combination with a DNA template that contains a site-specific ICL, represents a unique tool to study the molecular mechanism of ICL repair.
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Puck
authors.initials: P
authors.lastname: Knipscheer
authors.forename: Markus
authors.initials: M
authors.lastname: Räschle
authors.forename: Orlando D
authors.initials: OD
authors.lastname: Schärer
authors.forename: Johannes C
authors.initials: JC
authors.lastname: Walter
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004260:DNA Repair; D004261:DNA Replication; D009693:Nucleic Acid Hybridization; D010063:Ovum / Q000166:cytology*; D014981:Xenopus / Q000235:genetics*
chemical_list: D004247:DNA