title: Purification, crystallization and preliminary X-ray analysis of uracil-DNA glycosylase from Sulfolobus tokodaii strain 7.
abstract: Uracil-DNA glycosylase (UDG) specifically removes uracil from DNA by catalyzing hydrolysis of the N-glycosidic bond, thereby initiating the base-excision repair pathway. Although a number of UDG structures have been determined, the structure of archaeal UDG remains unknown. In this study, a deletion mutant of UDG isolated from Sulfolobus tokodaii strain 7 (stoUDGΔ) and stoUDGΔ complexed with uracil were crystallized and analyzed by X-ray crystallography. The crystals were found to belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 52.2, b = 52.3, c = 74.7 Å and a = 52.1, b = 52.2, c = 74.1 Å for apo stoUDGΔ and stoUDGΔ complexed with uracil, respectively.
authors.affiliation: Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
authors.forename: Akito
authors.initials: A
authors.lastname: Kawai
authors.forename: Shigesada
authors.initials: S
authors.lastname: Higuchi
authors.forename: Masaru
authors.initials: M
authors.lastname: Tsunoda
authors.forename: Kazuo T
authors.initials: KT
authors.lastname: Nakamura
authors.forename: Shuichi
authors.initials: S
authors.lastname: Miyamoto
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D016944:Sulfolobus / Q000201:enzymology*; D051981:Uracil-DNA Glycosidase / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D051981:Uracil-DNA Glycosidase