title: RNA expression of cytochrome P450 in Mexican women with breast cancer.
abstract: Involvement of cytochrome P450 genes (CYPs) in breast cancer (BCa) may differ between populations, with expression patterns affected by tumorigenesis. This may have an important role in the metabolism of anticancer drugs and in the progression of cancer. The aim of this study was to determine the mRNA expression patterns of four cytochrome P450 genes (CYP2W1, 3A5, 4F11 and 8A1) in Mexican women with breast cancer. Real- time PCR analyses were conducted on 32 sets of human breast tumors and adjacent non-tumor tissues, as well as 20 normal breast tissues. Expression levels were tested for association with clinical and pathological data of patients. We found higher gene expression of CYP2W1, CYP3A5, CYP4F11 in BCa than in adjacent tissues and only low in normal mammary glands in our Mexican population while CYP8A1 was only expressed in BCa and adjacent tissues. We found that Ki67 protein expression was associated with clinicopathological features as well as with CYP2W1, CYP4F11 and CYP8A1 but not with CYP3A5. The results indicated that breast cancer tissues may be better able to metabolize carcinogens and other xenobiotics to active species than normal or adjacent non-tumor tissues.
authors.affiliation: Section of Research and Graduate Studies, Instituto Politecnico Nacional, SEDENA, Mexico.
authors.forename: Cindy
authors.initials: C
authors.lastname: Bandala
authors.forename: E
authors.initials: E
authors.lastname: Floriano-Sánchez
authors.forename: N
authors.initials: N
authors.lastname: Cárdenas-Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: López-Cruz
authors.forename: E
authors.initials: E
authors.lastname: Lara-Padilla
mesh_terms: D001943:Breast Neoplasms / Q000201:enzymology* / Q000235:genetics; D051544:Cytochrome P-450 CYP3A / Q000096:biosynthesis* / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D000072467:Cytochrome P450 Family 2; D000072470:Cytochrome P450 Family 4; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis; D008800:Mexico; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D019394:Ki-67 Antigen; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; C509336:CYP2W1 protein, human; C510164:CYP3A5 protein, human; D051544:Cytochrome P-450 CYP3A; D000072467:Cytochrome P450 Family 2; D000072470:Cytochrome P450 Family 4; C492722:CYP4F11 protein, human; C472456:PTGIS protein, human