title: Cloning, expression, crystallization and preliminary X-ray studies of the ferredoxin-NAD(P)+ reductase from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1.
abstract: Ferredoxin-NADP(+) reductase (FNR) is a flavoenzyme that catalyses the reduction of NADP(+) in the final step of the photosynthetic electron-transport chain. FNR from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 (TeFNR) contains an additional 9 kDa domain at its N-terminus relative to chloroplastic FNRs and is more thermostable than those from mesophilic cyanobacteria. With the aim of understanding the structural basis of the thermostability of TeFNR and assigning a structural role to the small additional domain, the gene encoding TeFNR with and without an additional domain was engineered for heterologous expression and the recombinant proteins were purified and crystallized. Crystals of TeFNR without the additional domain belonged to space group P2(1), with unit-cell parameters a = 55.05, b = 71.66, c = 89.73 Å, α = 90, β = 98.21, γ = 90°.
authors.affiliation: Lehrstuhl für Biochemie der Pflanzen, Ruhr-Universität Bochum, 44780 Bochum, Germany.
authors.forename: Pasqual
authors.initials: P
authors.lastname: Liauw
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Mashiba
authors.forename: Marta
authors.initials: M
authors.lastname: Kopczak
authors.forename: Katrin
authors.initials: K
authors.lastname: Wiegand
authors.forename: Norifumi
authors.initials: N
authors.lastname: Muraki
authors.forename: Hisako
authors.initials: H
authors.lastname: Kubota
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kawano
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikeuchi
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Hase
authors.forename: Matthias
authors.initials: M
authors.lastname: Rögner
authors.forename: Genji
authors.initials: G
authors.lastname: Kurisu
mesh_terms: D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D000458:Cyanobacteria / Q000201:enzymology*; D005287:Ferredoxin-NADP Reductase / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression
chemical_list: D005287:Ferredoxin-NADP Reductase