title: The yield of experimental yeast populations declines during selection.
abstract: The trade-off between growth rate and yield can limit population productivity. Here we tested for this life-history trade-off in replicate haploid and diploid populations of Saccharomyces cerevisiae propagated in glucose-limited medium in batch cultures for 5000 generations. The yield of single clones isolated from the haploid lineages, measured as both optical and population density at the end of a growth cycle, declined during selection and was negatively correlated with growth rate. Initially, diploid populations did not pay this cost of adaptation but haploidized after about 1000-3000 generations of selection, and this ploidy transition was associated with a decline in yield caused by reduced cell size. These results demonstrate the experimental evolution of a trade-off between growth rate and yield, caused by antagonistic pleiotropy, during adaptation in haploids and after an adaptive transition from diploidy to haploidy.
authors.affiliation: Department of Biology, Wake Forest University, Winston-Salem, NC 27106, USA. jnjasmin@gmail.com
authors.forename: Jean-Nicolas
authors.initials: JN
authors.lastname: Jasmin
authors.forename: Marcus M
authors.initials: MM
authors.lastname: Dillon
authors.forename: Clifford
authors.initials: C
authors.lastname: Zeyl
mesh_terms: D000222:Adaptation, Physiological; D005075:Biological Evolution; D018533:Biomass; D004171:Diploidy; D006238:Haploidy; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development*; D012641:Selection, Genetic