title: Comparing linkage and association analyses in sheep points to a better way of doing GWAS.
abstract: Genome wide association studies (GWAS) have largely succeeded family-based linkage studies in livestock and human populations as the preferred method to map loci for complex or quantitative traits. However, the type of results produced by the two analyses contrast sharply due to differences in linkage disequilibrium (LD) imposed by the design of studies. In this paper, we demonstrate that association and linkage studies are in agreement provided that (i) the effects from both studies are estimated appropriately as random effects, (ii) all markers are fitted simultaneously and (iii) appropriate adjustments are made for the differences in LD between the study designs. We demonstrate with real data that linkage results can be predicted by the sum of association effects. Our association study captured most of the linkage information because we could predict the linkage results with moderate accuracy. We suggest that the ability of common single nucleotide polymorphism (SNP) to capture the genetic variance in a population will depend on the effective population size of the study organism. The results provide further evidence for many loci of small effect underlying complex traits. The analysis suggests a more informed method for GWAS is to fit statistical models where all SNPs are analysed simultaneously and as random effects.
authors.affiliation: Department of Agriculture and Food, University of Melbourne, Parkville, Victoria 3010, Australia. kathryn.kemper@dpi.vic.giv.au
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Kemper
authors.forename: Hans D
authors.initials: HD
authors.lastname: Daetwyler
authors.forename: Peter M
authors.initials: PM
authors.lastname: Visscher
authors.forename: Michael E
authors.initials: ME
authors.lastname: Goddard
mesh_terms: D000818:Animals; D008040:Genetic Linkage; D055106:Genome-Wide Association Study / Q000379:methods*; D015810:Linkage Disequilibrium; D015233:Models, Statistical; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci; D012756:Sheep / Q000235:genetics*