title: [Features of legionella biofilm formation in artificial and natural water systems].
abstract: OBJECTIVE
  Study the ability to form monospecies and associative biofilms as a characteristic oflegionella strains and features of organization of natural legionella biofilms in potentially dangerous water systems.
  
  
  METHODS
  Comparative evaluation ofthe ability of 28 strains of Legionella spp. to form biofilms was determined in water according to previously developed procedure. Samples from biofilm of industrial enterprise coolers and systems of hot water supply of public buildings (hotels, trade centers, hospitals) were studied. Biofilms were studied by scanning and transmission electron microscopy methods.
  
  
  RESULTS
  Legionella strains are divided into 3 groups by the ability to form biofilms. L. pneumophila BLR-05 strain that has the most pronounced ability to form monospecies biofilm and persistence in association with Pseudomonas aeruginosa was detected. Formation of massive legionella biofilm in association with bacteria of other taxonomic groups was detected on protective antibacterial filters in the system of hot water supply of a department of a therapeutic prophylaxis institution in the course of 2-3 weeks. Legionella biofilms on the surface of coolers resemble an aggregate of fungi, bacteria and blue-green algae enclosed into matrix.
  
  
  CONCLUSIONS
  The ability to form artificial monospecies and associative biofilm may be a useful characteristic of legionella strains for evaluation of their adhesion and be used to evaluate epidemiological significance of the isolated strains. Prevention of formation of natural legionella biofilms in potentially dangerous water systems is necessary as an essential component of modern strategy of legionellosis prophylaxis.
authors.forename: Iu E
authors.initials: IuE
authors.lastname: Dronina
authors.forename: T I
authors.initials: TI
authors.lastname: Karpova
authors.forename: O V
authors.initials: OV
authors.lastname: Sadretdinova
authors.forename: L V
authors.initials: LV
authors.lastname: Didenko
authors.forename: I S
authors.initials: IS
authors.lastname: Tartakovskiĭ
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D004323:Drainage, Sanitary; D005618:Fresh Water / Q000382:microbiology*; D006801:Humans; D007875:Legionella / Q000254:growth & development / Q000302:isolation & purification; D016952:Legionella pneumophila / Q000254:growth & development* / Q000302:isolation & purification; D007876:Legionellosis / Q000517:prevention & control; D008855:Microscopy, Electron, Scanning; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D014871:Water Microbiology; D014881:Water Supply