title: Influence of methylenetetrahydrofolate reductase C677T gene polymorphisms in Algerian infertile men with azoospermia or severe oligozoospermia.
abstract: OBJECTIVE
  The C677T allele of the methylenetetrahydrofolate reductase (MTHFR) gene has been suggested to represent a risk factor for male infertility. To confirm this association, the distribution of the single-nucleotide polymorphism C677T was investigated in idiopathic infertile Algerian patients with nonobstructive azoospermia (NOA) or severe oligoasthenoteratozoospermia (OAT). A case-control study was carried out, including 74 idiopathic infertile Algerian patients with NOA (n=46) or severe OAT (n=28) and 84 fertile men as controls. Polymorphism C677T was studied by polymerase chain reaction-restriction fragment length polymorphism, and the results were statistically analyzed.
  
  
  RESULTS
  The frequency of genotypes MTHFR 677CC, 677CT, and 677TT in idiopathic infertile men with NOA was 43.48%, 41.30%, and 15.22%; 39.29%, 50%, and 10.71% regarding the severe oligozoospermic men; and 42.86%, 45.24%, and 11.90% in the control group.
  
  
  CONCLUSIONS
  The data suggest that the C677T MTHFR polymorphism is not a risk factor for idiopathic male subfertility in an Algerian population.
authors.affiliation: Faculty of Natural and Life Sciences, Department of Animal Biology, Laboratory of Biology and Molecular Genetics, University Mentouri Constantine, Constantine, Algeria. rezgounedj@yahoo.fr
authors.forename: Djalila
authors.initials: D
authors.lastname: Chellat
authors.forename: Mohamed Larbi
authors.initials: ML
authors.lastname: Rezgoune
authors.forename: Douadi
authors.initials: D
authors.lastname: Hamane
authors.forename: Ouarda
authors.initials: O
authors.lastname: Semmame
authors.forename: Cherifa
authors.initials: C
authors.lastname: Benlatrèche
authors.forename: Noureddine
authors.initials: N
authors.lastname: Abadi
authors.forename: Dalila
authors.initials: D
authors.lastname: Satta
mesh_terms: D000462:Algeria; D000483:Alleles; D001483:Base Sequence; D016022:Case-Control Studies; D017931:DNA Primers; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers; D042965:Methylenetetrahydrofolate Reductase (NADPH2)