title: Differences in surface marker expression and chondrogenic potential among various tissue-derived mesenchymal cells from elderly patients with osteoarthritis.
abstract: Mesenchymal stem cells (MSCs) are self-renewing, multipotent cells that could potentially be used to repair injured cartilage in diseases such as osteoarthritis (OA). In this study we used bone marrow, adipose tissue from articular and subcutaneous locations, and synovial fluid samples from 18 patients with knee OA to find a suitable alternative source for the isolation of MSCs with high chondrogenic potential. MSCs from all tissues analysed had a fibroblastic morphology, but their rates of proliferation varied. Subcutaneous fat-derived MSCs proliferated faster than bone marrow- and Hoffa's fat pad-derived MSCs, while synovial fluid-derived MSCs grew more slowly. CD36 and CD54 expression was similar across all groups of MSCs with several minor differences. High expression of these surface markers in subcutaneous fat-derived MSCs was correlated with poor differentiation into hyaline cartilage. Synovial fluid-derived MSCs presented a relatively small chondrogenic differentiation capacity while Hoffa's fat pad-derived MSCs had strong chondrogenic potential. In conclusion, MSCs from elderly patients with OA may still display significant chondrogenic potential, depending on their origin.
authors.affiliation: Instituto Aragonés de Ciencias de la Salud, Zaragoza, España.
authors.forename: Elena
authors.initials: E
authors.lastname: Alegre-Aguarón
authors.forename: Paula
authors.initials: P
authors.lastname: Desportes
authors.forename: Felícito
authors.initials: F
authors.lastname: García-Álvarez
authors.forename: Tomás
authors.initials: T
authors.lastname: Castiella
authors.forename: Luis
authors.initials: L
authors.lastname: Larrad
authors.forename: María José
authors.initials: MJ
authors.lastname: Martínez-Lorenzo
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology; D000368:Aged; D000954:Antigens, Surface / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D018955:CD36 Antigens / Q000378:metabolism*; D002358:Cartilage, Articular / Q000166:cytology / Q000502:physiology; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000473:pathology* / Q000601:surgery; D013582:Synovial Fluid / Q000166:cytology
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D018955:CD36 Antigens; D018799:Intercellular Adhesion Molecule-1