title: Evaluation of pressurized liquid extraction for determination of organophosphorus and pyrethroid pesticides in soybean.
abstract: The purpose of this study was to develop an analytical method for the determination of organophosphorus and pyrethroid pesticides in soybean by pressurized liquid extraction (PLE). Two organic solvents, acetone and acetonitrile, were evaluated as extraction solvents. In both cases, the amount of extract was enhanced with increasing extraction temperature. The extracts obtained using acetonitrile were measured by gas chromatography/mass spectrometry after a cleanup process based on the analytical method for the Japanese Positive List System for Agricultural Chemicals Remaining in Foods. The effect of extraction temperature (range: 40- 130°C) on extraction efficiency was evaluated by a recovery study using 21 organophosphorus pesticides and 10 pyrethroid pesticides as target analytes and acetonitrile as the solvent. The results indicated that at 130°C, some organophosphorus pesticides might be degraded, whereas extraction temperatures between 70°C and 100°C were optimal. Next, a prepared sample containing fenitrothion and permethrin was analyzed. Although the sample was not soaked in water prior to analysis, PLE provided analytical results comparable to those obtained by solvent extraction with homogenization. Therefore, PLE is considered a simple and alternative technique for the extraction of organophosphorus and pyrethroid pesticides in soybean.
authors.affiliation: National Metrology Institute of Japan-NMIJ, National Institute of Advanced Industrial Science and Technology-AIST, Ibaraki, Japan. t-yarita@aist.go.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Yarita
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Aoyagi
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Otake
mesh_terms: D005506:Food Contamination / Q000032:analysis*; D006358:Hot Temperature; D059625:Liquid-Liquid Extraction / Q000295:instrumentation / Q000379:methods*; D009943:Organophosphorus Compounds / Q000032:analysis / Q000302:isolation & purification*; D010575:Pesticides / Q000032:analysis / Q000302:isolation & purification*; D011312:Pressure; D011722:Pyrethrins / Q000032:analysis / Q000302:isolation & purification*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D009943:Organophosphorus Compounds; D010575:Pesticides; D011722:Pyrethrins