title: Mutational Status of FGFR3 in Oral Squamous Cell Carcinoma.
abstract: OBJECTIVE
  Head and neck squamous cell carcinoma, including oral squamous cell carcinoma (OSCC) is the sixth most common cancer in the human population. Despite significant efforts committed in treatment of OSCC the overall survival rate of OSCC has not improved significantly. Activating mutations in the fibroblast growth factor receptor 3 (FGFR3) genes are responsible for some human cancers, including bladder and cervical carcinoma. Despite a high frequency in some benign skin disorders, FGFR3 mutations have not been reported in cutaneous malignancies. Therefore, FGFR3 gene may play a role in epithelial biology and mutations of FGFR3 gene may contribute to the development of OSCC.
  
  
  METHODS
  In this cross-sectional study, DNA was extracted and purified from snap frozen tissue biopsy sections of 20 OSCC cases. Exons 7 and 15 were amplified by polymerase chain reaction (PCR) and sequenced in both directions.
  
  
  RESULTS
  In three cases silent mutations were identified in exon 7 (882 T to C) which may be introduced as Single Nucleotide Polymorphism (SNP) and no mutation was identified in exon 15.
  
  
  CONCLUSIONS
  FGFR3 gene mutation in exon 7 and 15 has no significant role in the development and progression of OSCC. Analyzing other exons or considering other advanced gene mutation assessment techniques may clarify the role of this receptor mutation in OSCC pathogenesis.
authors.affiliation: Assistant Professor, Dental Research Center of Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: P
authors.initials: P
authors.lastname: Motahhary
authors.forename: F
authors.initials: F
authors.lastname: Baghaie
authors.forename: S
authors.initials: S
authors.lastname: Mamishi
authors.forename: B
authors.initials: B
authors.lastname: Pourakbari
authors.forename: S
authors.initials: S
authors.lastname: Mahmoudi
authors.forename: P Amini
authors.initials: PA
authors.lastname: Shakib