title: Nucleotide sequencing and phylogenetic analysis of the 3' region of glycoprotein C gene of South American bovine herpesviruses 1 and 5.
abstract: We herein describe a molecular analysis based on the 3' region of the glycoprotein C gene of 45 bovine herpesvirus (BoHV) isolates from Brazil (n=41), Uruguay (n=2) and Argentina (n=2). Nucleotide (nt) sequencing and alignment of 333 nt revealed levels of similarity ranging from 99.1% to 100% among BoHV-1 sequences (n=12); 96.2-100% among BoHV-5 sequences (n=32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The isolate SV 453/93 (BoHV-1 associated with genital disease) could not be included within BoHV-1 subtypes since it presented a markedly distinct nt and amino acid (aa) deduced sequences. A transmembrane domain of 24 aa and the putative cytoplasmic tail of 8 aa were identified and mapped. These results indicate that this genome region represents a suitable target for phylogenetic subgrouping of BoHV-1 and BoHV-5 isolates and, perhaps, for understanding evolutionary relationships.
authors.affiliation: Setor de Virologia, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Carolina Kist
authors.initials: CK
authors.lastname: Traesel
authors.forename: Mariana
authors.initials: M
authors.lastname: Sá e Silva
authors.forename: Fernando Rosado
authors.initials: FR
authors.lastname: Spilki
authors.forename: Rudi
authors.initials: R
authors.lastname: Weiblen
authors.forename: Eduardo Furtado
authors.initials: EF
authors.lastname: Flores
mesh_terms: D000818:Animals; D001118:Argentina; D001483:Base Sequence; D001938:Brazil; D002417:Cattle / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D025881:Herpesvirus 5, Bovine / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014583:Uruguay; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins; C038535:glycoprotein gC, herpes simplex virus type 1