title: Enhanced bioethanol production from pretreated corn stover via multi-positive effect of casein micelles.
abstract: Casein polypeptides containing substructures of αs1-casein, β-casein, k-casein, αs2-casein were used as a lignin-blocker at above critical micelles concentration to improve the bioethanol production of dilute acid, lime, alkali, extrusion and AFEX pretreated corn stover (CS). Application of 0.5 g/g glucan of casein was found to effectively increase the glucose yield of CS pretreated with dilute acid, lime, alkali, extrusion and AFEX by 31.9%, 17.0%, 22.7%, 29.5%, and 17.4%, respectively with no positive impact on Avicel. Consequently 96 h simultaneous saccharification and fermentation (SSF) of these hydrolysates reduced the fermentation period by up to 48 h and increased the theoretical yield of ethanol by 8.48-33.7% compared to control. Application of casein during saccharification reduced the enzyme utilization by 33.0%. Recycling of hydrolysate from casein-treated CS for a 2nd round hydrolysis resulted in average glucose yield of 36.4% compared to 29.0% control.
authors.affiliation: Department of Agricultural and Biosystems Engineering, 1400 North Campus Drive, South Dakota State University, Brookings, SD 57007, USA. Anahita.Eckard@sdstate.edu
authors.forename: Anahita Dehkhoda
authors.initials: AD
authors.lastname: Eckard
authors.forename: Kasiviswanathan
authors.initials: K
authors.lastname: Muthukumarappan
authors.forename: William
authors.initials: W
authors.lastname: Gibbons
mesh_terms: D000468:Alkalies / Q000494:pharmacology; D056804:Biofuels / Q000032:analysis*; D001709:Biotechnology / Q000379:methods*; D017610:Calcium Compounds / Q000494:pharmacology; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002364:Caseins / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D007526:Isoelectric Point; D008823:Micelles; D010087:Oxides / Q000494:pharmacology; D010316:Particle Size; D012212:Rheology / Q000187:drug effects; D012995:Solubility / Q000187:drug effects; D013696:Temperature; D014866:Waste Products / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D000468:Alkalies; D056804:Biofuels; D017610:Calcium Compounds; D002364:Caseins; D008823:Micelles; D010087:Oxides; D014866:Waste Products; D000431:Ethanol; C016538:lime; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose