title: Epidemiological study of HPV in oral mucosa through PCR.
abstract: The Human Papillomavirus (HPV) belongs to the Papillomaviridae family and has a capsid and a single DNA strand. Its infection occurs mainly through sexual intercourse, having an important tropism for skin and mucosal cells.
  
  
  OBJECTIVE
  To evaluate the HPV presence in normal oral mucosa of asymptomatic subjects and; in parallel, to correlate social behavioral habits with the virus.
  
  
  METHODS
  Contemporary cohort cross-sectional study. The HPV was found by PCR, using general primers MY09/11 in 125 oral mucosa samples submitted to DNA extraction and PCR to search for the beta-globin gene in order to assess the quality of the extracted DNA. In parallel, we carried out a study of behavioral issues associated with the patients.
  
  
  RESULTS
  All the samples had a positive diagnosis of the beta-hemoglobin gene. HPV was diagnosed in 23.2% of the samples analyzed.
  
  
  CONCLUSIONS
  The virus was present in 29 of the 125 patients, without them having any clinical-pathological manifestation associated with the HPV. As to the social behavior of the patients, we concluded that oral sex is statistically correlated to the virus, and besides the HPV has been statistically more present in female patients.
authors.affiliation: Departamento de Obstetrícia e Ginecologia, Faculdade de Medicina, Universidade de São Paulo.
authors.forename: Willys
authors.initials: W
authors.lastname: Tristão
authors.forename: Rodrigo Metzker Pereira
authors.initials: RM
authors.lastname: Ribeiro
authors.forename: Camila Andrea de
authors.initials: CA
authors.lastname: Oliveira
authors.forename: Julio Cesar
authors.initials: JC
authors.lastname: Betiol
authors.forename: Jussara de Sousa Ribeiro
authors.initials: Jde S
authors.lastname: Bettini
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000821:virology*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D012725:Sexual Behavior / Q000706:statistics & numerical data*; D055815:Young Adult; D055544:beta-Globins / Q000032:analysis*
chemical_list: D004279:DNA, Viral; D055544:beta-Globins