title: Anti-inflammatory and anti-allergic effect of Agaricus blazei extract in bone marrow-derived mast cells.
abstract: In this study, the anti-inflammatory and anti-allergic effects of the chloroform-soluble extract of Agaricus blazei in mouse bone marrow-derived mast cells (BMMCs) were investigated. The chloroform-soluble extract inhibited IL-6 production in PMA plus A23187-stimulated BMMCs, and down-regulated the phosphorylation of Akt. In addition, this extract demonstrated inhibition of the degranulation of β-hexosaminidase and the production of IL-6, prostaglandin D(2) and leukotriene C(4) in PMA plus A23187-induced BMMCs. In conclusion, the chloroform-soluble extract of Agaricus blazei exerted anti-inflammatory and anti-allergic activities mediated by influencing IL-6, prostaglandin D(2), leukotriene C(4), and the phosphorylation of Akt.
authors.affiliation: Immune Modulator Research Center, Bio-Therapeutics Research Institute, Korea Research Institute of Bioscience and Biotechnology, ChungBuk,  Korea.
authors.forename: Hyuk-Hwan
authors.initials: HH
authors.lastname: Song
authors.forename: Hee-Sung
authors.initials: HS
authors.lastname: Chae
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
authors.forename: Hyeong-Kyu
authors.initials: HK
authors.lastname: Lee
authors.forename: Young-Won
authors.initials: YW
authors.lastname: Chin
mesh_terms: D000364:Agaricus; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001688:Biological Products / Q000494:pharmacology / Q000627:therapeutic use*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects; D015536:Down-Regulation; D005260:Female; D007249:Inflammation / Q000188:drug therapy* / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis*; D017997:Leukotriene C4 / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010766:Phosphorylation; D008517:Phytotherapy; D015230:Prostaglandin D2 / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D000893:Anti-Inflammatory Agents; D001688:Biological Products; D015850:Interleukin-6; D017997:Leukotriene C4; D000001:Calcimycin; D051057:Proto-Oncogene Proteins c-akt; D001619:beta-N-Acetylhexosaminidases; D013755:Tetradecanoylphorbol Acetate; D015230:Prostaglandin D2