title: The association of elevated 2',5'-oligoadenylate-dependent RNase L with lung cancer correlated with deficient enzymatic activity and decreased capacity of RNase L dimerization.
abstract: RNase L mediates critical cellular functions including antiviral, proapoptotic, antiproliferative and tumor suppressive activities. In this study, the expression and function of RNase L in lung cancer cells were examined. Interestingly we have found that the expression of RNase L in lung cancer cells was 3- and 9-fold higher in its mRNA and protein levels, but a significant decrease of its enzymatic activity when compared to that in corresponding normal lung cells. Further investigation revealed that 2-5A-induced dimerization of the RNase L protein, a necessary prerequisite for activation of RNase L, was inhibited, as a result of that RLI, a specific inhibitor of RNase L, was remarkably up-regulated in the cancer cells. Our findings provide new insight into how cancer cells escape normal growth-regulating mechanisms to form a tumor and the information may be useful for the design of novel strategies for treating lung cancer through regulating RNase L activity.
authors.affiliation: Department of Immunology, Tongji University School of Medicine, 1239 Siping Road, Shanghai 200092, China. yhj_an@yahoo.com.cn
authors.forename: Huijing
authors.initials: H
authors.lastname: Yin
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhou
authors.forename: Yalei
authors.initials: Y
authors.lastname: Dai
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology; D045744:Cell Line, Tumor; D004722:Endoribonucleases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000235:genetics; D009843:Oligoribonucleotides / Q000494:pharmacology; D055503:Protein Multimerization / Q000187:drug effects / Q000235:genetics; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology
chemical_list: D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D011500:Protein Synthesis Inhibitors; C023505:2',5'-oligoadenylate; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease