title: Dopamine-assisted immobilization of hydroxyapatite nanoparticles and RGD peptides to improve the osteoconductivity of titanium.
abstract: Hydroxyapatite (HAp) coating on orthopedic implants is a common strategy to increase osteointegration. In this work, a facile deposition method based on dopamine polymerization was developed for preparation of HAp-coated titanium substrates for orthopedic applications. Nanostructured HAp was mixed with an alkaline dopamine solution and then deposited onto titanium to form a dopamine/HAp ad-layer. The deposition of dopamine/HAp greatly enhanced the adhesion, proliferation, and mineralization of osteoblasts. Furthermore, RGD-containing peptides were immobilized to dopamine/HAp coated titanium and further enhanced cell adhesion and osteogenic differentiation. In conclusion, this facile dopamine-assisted surface modification method shows a great potential for orthopedic and dental applications.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Chih-Yuan
authors.initials: CY
authors.lastname: Chien
authors.forename: Tse-Ying
authors.initials: TY
authors.lastname: Liu
authors.forename: Wei-Hsuan
authors.initials: WH
authors.lastname: Kuo
authors.forename: Meng-Jiy
authors.initials: MJ
authors.lastname: Wang
authors.forename: Wei-Bor
authors.initials: WB
authors.lastname: Tsai
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible; D004298:Dopamine / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D051381:Rats; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D017886:Durapatite; D014025:Titanium; D004298:Dopamine