title: Defense peptides from barnyard grass (Echinochloa crusgalli L.) seeds.
abstract: A number of defense polypeptides from latent seeds of weed cereal barnyard grass (Echinochloa crusgalli L.) has been isolated and characterized using an acidic extraction and high performance liquid chromatography methods in combination with MALDI-TOF mass spectrometry and Edman sequencing. Members of three antimicrobial peptide families and two protease inhibitor families were found to be localized in barnyard grass seeds. Their biological activity concerning to Gram-Positive and Gram-Negative phytopathogenic bacteria, as well as oomycete Phytophthora infestans, has been investigated. Diversity of barnyard grass defense peptides is a significant factor that provides a resistance of E. crusgalli seeds to germination and latent phases.
authors.affiliation: Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russian Federation. rea21@list.ru
authors.forename: E A
authors.initials: EA
authors.lastname: Rogozhin
authors.forename: D Y
authors.initials: DY
authors.lastname: Ryazantsev
authors.forename: E V
authors.initials: EV
authors.lastname: Grishin
authors.forename: T A
authors.initials: TA
authors.lastname: Egorov
authors.forename: S K
authors.initials: SK
authors.lastname: Zavriev
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031747:Echinochloa / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000472:pathogenicity; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000472:pathogenicity; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D055750:Phytophthora infestans / Q000187:drug effects / Q000472:pathogenicity; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000737:chemistry / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D011198:Solanum tuberosum / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D010940:Plant Proteins; D011480:Protease Inhibitors