title: Evaluating the potential role of pomegranate peel in aluminum-induced oxidative stress and histopathological alterations in brain of female rats.
abstract: Studies have shown that pomegranate, Punica granatum Linn. (Lythraceae), has remarkable biological and medicinal properties. However, the effects of pomegranate peel methanolic extract (PPME) on the aluminum-induced oxidative stress and histopathological change have not been reported yet. To determine the effect of PPME (200 mg/kg bwt) on the aluminum chloride (AlCl₃; 34 mg/kg bwt)-induced neurotoxicity, aluminum accumulation in brain and oxidant/antioxidant status were determined. The change of brain structure was investigated with hematoxylin and eosin, and anti-apoptosis effects of PPME were analyzed by immunohistochemistry. The present study showed an indication of carcinogenicity in the AlCl₃-treated group representing an increase in tissue tumor markers such as tumor necrosis factor-α and angiogenin and inflammation by inducing an increase in prostaglandin E2 and prostaglandin F2α. PPME protected brain through decreasing the aluminum accumulation and stimulating antioxidant activities and anti-apoptotic proteins namely Bcl-2. Therefore, these results indicated that pomegranate peel methanolic extract could inhibit aluminum-induced oxidative stress and histopathological alternations in brain of female rats, and these effects may be related to anti-apoptotic and antioxidants activities.
authors.affiliation: Biomedical Research Center, Health Sciences Technology Park, University of Granada, Avda. del Conocimiento s/n, 18100 Armilla, Granada, Spain. aest1977@hotmail.com
authors.forename: Ahmed E
authors.initials: AE
authors.lastname: Abdel Moneim
mesh_terms: D000535:Aluminum / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000008:administration & dosage; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D002274:Carcinogens, Environmental / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D002712:Chlorides / Q000008:administration & dosage; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005638:Fruit / Q000737:chemistry; D029561:Lythraceae / Q000737:chemistry*; D009474:Neurons / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D020258:Neurotoxicity Syndromes / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D017607:Aluminum Compounds; D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; D002274:Carcinogens, Environmental; D002712:Chlorides; D016207:Cytokines; D010936:Plant Extracts; D000077410:Aluminum Chloride; D000535:Aluminum