title: A high-coverage genome sequence from an archaic Denisovan individual.
abstract: We present a DNA library preparation method that has allowed us to reconstruct a high-coverage (30×) genome sequence of a Denisovan, an extinct relative of Neandertals. The quality of this genome allows a direct estimation of Denisovan heterozygosity indicating that genetic diversity in these archaic hominins was extremely low. It also allows tentative dating of the specimen on the basis of "missing evolution" in its genome, detailed measurements of Denisovan and Neandertal admixture into present-day human populations, and the generation of a near-complete catalog of genetic changes that swept to high frequency in modern humans since their divergence from Denisovans.
authors.affiliation: Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, D-04103 Leipzig, Germany. mmeyer@eva.mpg.de
authors.forename: Matthias
authors.initials: M
authors.lastname: Meyer
authors.forename: Martin
authors.initials: M
authors.lastname: Kircher
authors.forename: Marie-Theres
authors.initials: MT
authors.lastname: Gansauge
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Fernando
authors.initials: F
authors.lastname: Racimo
authors.forename: Swapan
authors.initials: S
authors.lastname: Mallick
authors.forename: Joshua G
authors.initials: JG
authors.lastname: Schraiber
authors.forename: Flora
authors.initials: F
authors.lastname: Jay
authors.forename: Kay
authors.initials: K
authors.lastname: Prüfer
authors.forename: Cesare
authors.initials: C
authors.lastname: de Filippo
authors.forename: Peter H
authors.initials: PH
authors.lastname: Sudmant
authors.forename: Can
authors.initials: C
authors.lastname: Alkan
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Fu
authors.forename: Ron
authors.initials: R
authors.lastname: Do
authors.forename: Nadin
authors.initials: N
authors.lastname: Rohland
authors.forename: Arti
authors.initials: A
authors.lastname: Tandon
authors.forename: Michael
authors.initials: M
authors.lastname: Siebauer
authors.forename: Richard E
authors.initials: RE
authors.lastname: Green
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bryc
authors.forename: Adrian W
authors.initials: AW
authors.lastname: Briggs
authors.forename: Udo
authors.initials: U
authors.lastname: Stenzel
authors.forename: Jesse
authors.initials: J
authors.lastname: Dabney
authors.forename: Jay
authors.initials: J
authors.lastname: Shendure
authors.forename: Jacob
authors.initials: J
authors.lastname: Kitzman
authors.forename: Michael F
authors.initials: MF
authors.lastname: Hammer
authors.forename: Michael V
authors.initials: MV
authors.lastname: Shunkov
authors.forename: Anatoli P
authors.initials: AP
authors.lastname: Derevianko
authors.forename: Nick
authors.initials: N
authors.lastname: Patterson
authors.forename: Aida M
authors.initials: AM
authors.lastname: Andrés
authors.forename: Evan E
authors.initials: EE
authors.lastname: Eichler
authors.forename: Montgomery
authors.initials: M
authors.lastname: Slatkin
authors.forename: David
authors.initials: D
authors.lastname: Reich
authors.forename: Janet
authors.initials: J
authors.lastname: Kelso
authors.forename: Svante
authors.initials: S
authors.lastname: Pääbo
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D005580:Fossils; D051456:Gene Flow; D015723:Gene Library; D014644:Genetic Variation; D015894:Genome, Human / Q000235:genetics*; D006579:Heterozygote; D006801:Humans; D008969:Molecular Sequence Data; D059125:Neanderthals / Q000235:genetics*; D017422:Sequence Analysis, DNA