title: [Effect of perioperative intestinal probiotics on intestinal flora and immune function in patients with colorectal cancer].
abstract: OBJECTIVE
  To investigate the effect of perioperative application of intestinal probiotics to substitute oral intestinal antimicrobial agents on intestinal flora and immune function in surgical patients with colorectal cancer.
  
  
  METHODS
  Sixty patients with colorectal cancer undergoing elective laparoscopic radical surgery were randomized to receive preoperative bowel preparation using oral intestinal antimicrobial agents (n=20) or using oral intestinal probiotics (Jinshuangqi Tablets, 2.0 g, 3 times daily) since the fifth day before the operation and at 24 h after the operation for 7 consecutive days. Upon admission and 7 days after the operation, fecal samples and fasting peripheral venous blood were collected from the patients to examine the intestinal flora and serum levels of interleukin-2 (IL-2), IgA, IgG, and IgM, NK cell activity, T lymphocytes subsets CD3(+), CD4(+), CD8(+) and CD4(+)/CD8(+) ratio.
  
  
  RESULTS
  At 7 days after the operation, the patients receiving probiotics showed significantly increased counts of intestinal Bifidobacterium, Lactobacillus, and Enterococcus (P<0.05) and significantly lowered counts of Escherichia coli and Staphylococcus aureus (P<0.05). The serum levels of IL-2, IgA, IgG and IgM as well as CD4(+) cell percentage all increased significantly in probiotics group compared with those in patients with conventional intestinal preparation (P<0.05).
  
  
  CONCLUSIONS
  Perioperative application of intestinal probiotics to replace preoperative oral intestinal antimicrobial agents can effectively correct intestinal flora imbalance and improve the immune function of surgical patients with colorectal cancer.
authors.affiliation: Department of Gastrointestinal Surgery, Southern Medical University, Foshan, China. zhudajian123@sina.com
authors.forename: Dajian
authors.initials: D
authors.lastname: Zhu
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Chen
authors.forename: Jinhao
authors.initials: J
authors.lastname: Wu
authors.forename: Yongle
authors.initials: Y
authors.lastname: Ju
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Lu
authors.forename: Manzhao
authors.initials: M
authors.lastname: Ouyang
authors.forename: Baojun
authors.initials: B
authors.lastname: Ren
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000368:Aged; D001644:Bifidobacterium; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000382:microbiology*; D005260:Female; D006801:Humans; D007422:Intestines / Q000382:microbiology; D007432:Intraoperative Period; D008297:Male; D008875:Middle Aged; D011292:Premedication; D019936:Probiotics / Q000627:therapeutic use*; D011446:Prospective Studies; D016037:Single-Blind Method