title: Evaluation of a fosmid-clone-based microarray for comparative analysis of swine fecal metagenomes.
abstract: Glass slide arrayed with fosmid clone DNAs generated from swine feces as probes were fabricated and used as a metagenome microarray (MGA). MGA appeared to be specific to their corresponding target genomic fragments. The detection limit was 10 ng of genomic DNA (ca. 10(6) bacterial cells) in the presence of 1000 ng of background DNA. Linear relationships between the signal intensity and the target DNA (20-100 ng) were observed (r ( 2 )=0.98). Application of MGA to the comparison of swine fecal metagenomes suggested that the microbial community composition of swine intestine could be dependent on the health state of swine.
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Soo-Je
authors.initials: SJ
authors.lastname: Park
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Kim
authors.forename: Man-Young
authors.initials: MY
authors.lastname: Jung
authors.forename: So-Jeong
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hongik
authors.initials: H
authors.lastname: Kim
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
authors.forename: Jong-Chan
authors.initials: JC
authors.lastname: Chae
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Rhee
mesh_terms: D000818:Animals; D005144:F Factor; D005243:Feces / Q000382:microbiology*; D005822:Genetic Vectors; D054892:Metagenome; D046228:Microarray Analysis / Q000379:methods*; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013552:Swine / Q000382:microbiology*