title: Suppression of lipopolysaccharide and galactosamine-induced hepatic inflammation by red grape pomace.
abstract: Grape pomace is generated in the production process of wine and grape juices and is an industrial waste. This study investigated whether an intake of grape pomace was able to suppress chronic inflammation induced by lipopolysaccharide (LPS) and galactosamine (GalN) in vivo. When Sprague-Dawley rats were orally given methanolic extracts from red and white grape pomace, the extracts inhibited the LPS/GalN-evoked activation of nuclear factor-κB (NF-κB) dose-dependently, and red grape pomace exerted a stronger effect than white grape one. Next, rats were fed an AIN93 M-based diet containing 5% red grape pomace for 7 days, followed by the intraperitoneal injection of LPS and GalN. The intake of the red grape pomace-supplemented diet was found to suppress the LPS/GalN-induced activation of NF-κB and expression of inducible nitric oxide synthase and cyclooxygenase-2 proteins. These results suggest that red grape pomace may contain an abundance of effective compound(s) for anti-inflammatory action.
authors.affiliation: Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Nishiumi
authors.forename: Rie
authors.initials: R
authors.lastname: Mukai
authors.forename: Takashi
authors.initials: T
authors.lastname: Ichiyanagi
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ashida
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000276:immunology; D015536:Down-Regulation / Q000187:drug effects*; D005688:Galactosamine / Q000009:adverse effects* / Q000276:immunology; D006801:Humans; D007220:Industrial Waste / Q000032:analysis; D008070:Lipopolysaccharides / Q000009:adverse effects* / Q000276:immunology; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D008107:Liver Diseases / Q000188:drug therapy / Q000201:enzymology / Q000209:etiology / Q000276:immunology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000276:immunology; D010936:Plant Extracts / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027843:Vitis / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D007220:Industrial Waste; D008070:Lipopolysaccharides; D010936:Plant Extracts; D005688:Galactosamine; D019001:Nitric Oxide Synthase; D051546:Cyclooxygenase 2