title: Plasmacytoid dendritic cells control B cell-derived IL-10 production.
abstract: Plasmacytoid dendritic cells (pDC) exert contradictory roles: they represent major players in the pathogenesis of type I interferon-mediated autoimmunity but contribute to tolerance in the transplant setting. In this study we describe pDC as cellular enhancers of B cell-derived IL-10 production, a mechanism recently described as relevant in maintaining peripheral tolerance. Our data demonstrate that in human peripheral blood pDC augment IL-10 production in B cells in response to TLR7 and -9 ligands. They further show that pDC themselves produce IL-10 in response to TLR stimulation, most prominently after triggering of TLR7. Additionally, the data indicate that the positive regulatory effect of pDC on IL-10 production is not due to type I interferon production or other soluble factors. We conclude that pDC/B cell contact is essential for B cell-mediated immune suppression.
authors.affiliation: Department of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg, Heidelberg, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Georg
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bekeredjian-Ding
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002450:Cell Communication; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007108:Immune Tolerance; D016898:Interferon-alpha / Q000494:pharmacology; D016753:Interleukin-10 / Q000096:biosynthesis*; D051199:Toll-Like Receptor 7 / Q000378:metabolism*; D051217:Toll-Like Receptor 9 / Q000378:metabolism*
chemical_list: D016898:Interferon-alpha; C495348:TLR7 protein, human; C495371:TLR9 protein, human; D051199:Toll-Like Receptor 7; D051217:Toll-Like Receptor 9; D016753:Interleukin-10