title: High yield isolation of BMP-2 from bone and in vivo activity of a combination of BMP-2/TGF-β1.
abstract: A high-yield purification procedure for protein fractions derived from porcine bone matrix extracts is described, which has a high abundance of bone morphogenetic protein-2 (BMP-2). Naturally derived pBMP-2, ~5 μg per kilogram of porcine bone matrix, was isolated by using a 300 kDa membrane before chromatographic processing on heparin affinity media. The elution of pBMP-2 and transforming growth factor-β(1) (TGF-β(1)) revealed morphogen peaks that were unresolved on Prosep(®) medium, but resolved on hydroxyapatite medium. Antagonism was observed in animal studies when the two proteins were combined in specific doses. The TGF-β(1) fraction alone was not active in the rodent heterotopic in vivo bioassay, confirming previously obtained results.
authors.affiliation: Department of Pharmaceutical Sciences, Tshwane University of Technology, Pretoria, South Africa.
authors.forename: Sibusiso J
authors.initials: SJ
authors.lastname: Sibiya
authors.forename: Eugene I
authors.initials: EI
authors.lastname: Olivier
authors.forename: Nicolaas
authors.initials: N
authors.lastname: Duneas
mesh_terms: D000818:Animals; D001681:Biological Assay; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000302:isolation & purification; D001842:Bone and Bones / Q000737:chemistry*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D034421:Sus scrofa; D053773:Transforming Growth Factor beta1 / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D055396:Bone Morphogenetic Protein 2; D053773:Transforming Growth Factor beta1