title: High IL-10 production by aged AIDS patients is related to high frequency of Tr-1 phenotype and low in vitro viral replication.
abstract: This work aims to elucidate the effects of age and HIV-1 infection on the frequency and function of T cell subsets in response to HIV-specific and non-specific stimuli. As compared with the younger AIDS group, the frequencies of naive and central memory T cells were significantly lower in aged AIDS patients. Although there was also a dramatic loss of classical CD4(+)FoxP3(+)CD25(+)Treg cells in this patient group, high frequencies of IL-10-producing CD4(+)FoxP3(-) T cells were observed. In our system, the increased production of IL-10 in aged AIDS patients was mainly derived from Env-specific CD4(+)FoxP3(-)CD152(+) T cells. Interestingly, while the blockade of IL-10 activity by monoclonal antibody clearly enhanced the release of IL-6 and IL-1β by Env-stimulated PBMC cultures from aged AIDS patients, this monoclonal antibody enhanced in vitro HIV-1-replication. In conclusion, HIV infection and aging undoubtedly contribute synergistically to a complex immune dysfunction in T cell compartment of HAART-treated older HIV-infected individuals.
authors.affiliation: Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil.
authors.forename: Regis M
authors.initials: RM
authors.lastname: Andrade
authors.forename: Joana
authors.initials: J
authors.lastname: Hygino
authors.forename: Taissa M
authors.initials: TM
authors.lastname: Kasahara
authors.forename: Morgana M
authors.initials: MM
authors.lastname: Vieira
authors.forename: Luciana F
authors.initials: LF
authors.lastname: Xavier
authors.forename: Bernardo
authors.initials: B
authors.lastname: Blanco
authors.forename: Paulo V
authors.initials: PV
authors.lastname: Damasco
authors.forename: Rodrigo M
authors.initials: RM
authors.lastname: Silva
authors.forename: Dirce B
authors.initials: DB
authors.lastname: Lima
authors.forename: Ariane L
authors.initials: AL
authors.lastname: Oliveira
authors.forename: Alberto S
authors.initials: AS
authors.lastname: Lemos
authors.forename: Arnaldo F B
authors.initials: AF
authors.lastname: Andrade
authors.forename: Cleonice A M
authors.initials: CA
authors.lastname: Bento
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000188:drug therapy / Q000276:immunology* / Q000821:virology; D000328:Adult; D000367:Age Factors; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D023241:Antiretroviral Therapy, Highly Active; D000998:Antiviral Agents / Q000008:administration & dosage; D002478:Cells, Cultured; D005260:Female; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000276:immunology*; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000276:immunology; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; C508609:IL10 protein, human; C508600:IL6 protein, human; D053583:Interleukin-1beta; D015850:Interleukin-6; D016753:Interleukin-10