title: Structural and conformational dynamics of self-assembling bioactive β-sheet peptide nanostructures decorated with multivalent RNA-binding peptides.
abstract: Understanding the dynamic behavior of nanostructural systems is important during the development of controllable and tailor-made nanomaterials. This is particularly true for nanostructures that are intended for biological applications because biomolecules are usually highly dynamic and responsive to external stimuli. In this Article, we investigated the structural and conformational dynamics of self-assembling bioactive β-sheet peptide nanostructures using electron paramagnetic resonance (EPR) spectroscopy. The model peptide nanostructures are characterized by the cross-β spine of β-ribbon fibers and multiple RNA-binding bioactive peptides that constitute the shell of the nanostructures. We found first, that bioactive peptides at the shell of β-ribbon nanostructure have a mobility similar to that of an isolated monomeric peptide. Second, the periphery of the cross-β spine is more immobile than the distal part of surface-displayed bioactive peptides. Third, the rotational dynamics of short and long fibrils are similar; that is, the mobility is largely independent of the extent of aggregation. Fourth, peptides that constitute the shell are affected first by the external environment at the initial stage. The cross-β spine resists its external environment to a certain extent and abruptly disintegrates when the perturbation reaches a certain degree. Our results provide an overall picture of β-sheet peptide nanostructure dynamics, which should be useful in the development of dynamic self-assembled peptide nanostructures.
authors.affiliation: Translational Research Center for Protein Function Control and Department of Materials Science & Engineering, Yonsei University, Seoul 120-749, Korea.
authors.forename: Sanghun
authors.initials: S
authors.lastname: Han
authors.forename: Donghun
authors.initials: D
authors.lastname: Kim
authors.forename: So-hee
authors.initials: SH
authors.lastname: Han
authors.forename: Nam Hee
authors.initials: NH
authors.lastname: Kim
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Yong-beom
authors.initials: YB
authors.lastname: Lim
mesh_terms: D000595:Amino Acid Sequence; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D008969:Molecular Sequence Data; D049329:Nanostructures; D010455:Peptides / Q000737:chemistry*; D016601:RNA-Binding Proteins / Q000737:chemistry*
chemical_list: D010455:Peptides; D016601:RNA-Binding Proteins