title: The effects of cetrorelix and triptorelin on the viability and steroidogenesis of cultured human granulosa luteinized cells.
abstract: BACKGROUND
  We investigated the effects of the gonadotropin-releasing hormone (GnRH) agonist triptorelin as well the GnRH antagonist cetrorelix those of on the viability and steroidogenesis in human granulosa luteinized (hGL) cell cultures.
  
  
  METHODS
  The hGL cells were obtained from 34 women undergoing ovarian stimulation for IVF treatment. The cells were cultured for 48 h with or without 1 nM or 3 nM of cetrorelix or triptorelin in serum-free media. The cell viability was evaluated by the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] assay. The concentrations of estradiol and progesterone in culture supernatants were measured by ELISA.
  
  
  RESULTS
  Treatment with triptorelin slightly increased cell viability, whereas treatment with 3 nM cetrorelix led to a significant decrease. Estradiol concentrations were reduced with 3 nM triptorelin. Cultures treated with high-dose of either cetrorelix or triptorelin tended to secrete less progesterone than controls.
  
  
  CONCLUSIONS
  Cetrorelix significantly reduces the viability of hGL cells. Triptorelin and cetrorelix may have minor effects on steroidogenesis. These results suggest that GnRH analogues may influence ovarian functions.
authors.affiliation: Laboratory of Physiology, School of Medicine, Democritus University of Thrace, Dragana, 68100 Alexandroupolis, Greece. cmetalli@mbg.duth.gr
authors.forename: Chryssa
authors.initials: C
authors.lastname: Metallinou
authors.forename: Frank
authors.initials: F
authors.lastname: Köster
authors.forename: Klaus
authors.initials: K
authors.lastname: Diedrich
authors.forename: Nikos
authors.initials: N
authors.lastname: Nikolettos
authors.forename: Byron
authors.initials: B
authors.lastname: Asimakopoulos
mesh_terms: D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D004958:Estradiol / Q000096:biosynthesis* / Q000378:metabolism; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D006727:Hormone Antagonists / Q000494:pharmacology*; D006801:Humans; D011374:Progesterone / Q000096:biosynthesis / Q000378:metabolism; D017329:Triptorelin Pamoate / Q000494:pharmacology*
chemical_list: D006727:Hormone Antagonists; D017329:Triptorelin Pamoate; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; C062876:cetrorelix