title: Expression of myostatin in neural cells of the olfactory system.
abstract: Recent studies show that myostatin mRNA expression is found in some regions of the brain. However, the functional significance of this is currently unknown. We therefore investigated myostatin expression and function in the brain. In this study, we used immunohistochemistry, in situ hybridization, and RT-PCR analysis to reveal that myostatin is expressed in the mitral cells in the olfactory bulb (OB) and in neurons in the olfactory cortex (OC). Using 3D reconstruction, mitral cells positive for myostatin were positioned in the lateral and ventral regions of the OB. In contrast, myostatin-positive mitral cells were detected in mice at 2 weeks of age, but not on days 0 and 7 after birth. Activin receptor IIB, a myostatin receptor, was expressed in the OB, OC, hippocampus, and paraventricular thalamic nucleus. Moreover, c-Fos immunostaining in granule cells in the OB was augmented after intracerebroventricular injection of myostatin. These findings suggest that myostatin is localized in specific cells associated with the olfactory system of the brain and may act as a key inhibitor in cell and/or signal development of the olfactory system.
authors.affiliation: Laboratory of Functional Morphology, Department of Animal Biology, Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Iwasaki
authors.forename: Masato
authors.initials: M
authors.lastname: Miyake
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Eri
authors.initials: E
authors.lastname: Kitagawa
authors.forename: Kouichi
authors.initials: K
authors.lastname: Watanabe
authors.forename: Shyuichi
authors.initials: S
authors.lastname: Ohwada
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
authors.forename: Michael T
authors.initials: MT
authors.lastname: Rose
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
mesh_terms: D030301:Activin Receptors, Type II / Q000378:metabolism; D028341:Activins / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D005786:Gene Expression Regulation / Q000187:drug effects; D007276:Injections, Intraventricular; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D055435:Myostatin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D009830:Olfactory Bulb / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009833:Olfactory Pathways / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D055435:Myostatin; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; C422405:activin A; D028341:Activins; D030301:Activin Receptors, Type II; C425584:activin receptor type II-B