title: [Persistence of Pseudomonas aeruginosa strains in patients of Federal Scientific Center of Transplantology and Artificial Organs].
abstract: OBJECTIVE
  Study genetic diversity of P. aeruginosa strains persisting in patients of Federal Scientific Center of Transplantology and Artificial Organs, and main factors facilitating persistence of strains in the hospital.
  
  
  METHODS
  136 P. aeruginosa strains isolated from patients of the center for 3 years 6 months were genotyped by RAPD-PCR and MLST methods and studied for antibiotics resistance and presence of integrons.
  
  
  RESULTS
  Genetic diversity of strains persisting in hospital was established. Strains of main genotypes ST235, ST446, ST598 were isolated from patients of various surgical departments. Patients were shown to be colonized by these strains during stay in reanimation and intensive therapy department (RITD) of the hospital. Strains of dominant genotype 235 were isolated from 47% of examined patients during more than 3 years. Only genotype 235 strains contained integron with cassettes of antibiotics resistance genes blaGES5 and aadA6 in the genome.
  
  
  CONCLUSIONS
  The data obtained show that over the period of observation in the center 1 clone of P. aeruginosa that belonged to genotype 235 dominated. This clone was endemic for this hospital and in the process of prolonged persistence became more resistant to antibiotics. Colonization of patients with these strains occurs in RITD. This confirms the necessity of constant monitoring of hospital microflora for advance detection of potentially dangerous epidemic hospital strains able to cause hospital infections.
authors.forename: L R
authors.initials: LR
authors.lastname: Avetisian
authors.forename: O L
authors.initials: OL
authors.lastname: Voronina
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Chernukha
authors.forename: M S
authors.initials: MS
authors.lastname: Kunda
authors.forename: N I
authors.initials: NI
authors.lastname: Gabrielian
authors.forename: V G
authors.initials: VG
authors.lastname: Lunin
authors.forename: I A
authors.initials: IA
authors.lastname: Shaginian
mesh_terms: D000046:Academic Medical Centers; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D032023:Integrons / Q000235:genetics; D007362:Intensive Care Units; D058885:Multilocus Sequence Typing; D016377:Organ Transplantation; D010802:Phylogeny; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000472:pathogenicity; D019105:Random Amplified Polymorphic DNA Technique; D012426:Russia
chemical_list: D000900:Anti-Bacterial Agents; D017931:DNA Primers; D004269:DNA, Bacterial