title: Effect of immunization with a recombinant cholera toxin B subunit/somatostatin fusion protein on immune response and growth hormone levels in mice.
abstract: Somatostatin (SS) is a hormone that inhibits growth hormone secretion. Cholera toxin B subunit (CTB) is a widely used adjuvant to improve the immunogenicity of co-administrated antigen. To block the growth hormone-inhibiting effect of SS, a fusion gene of CTB and SS was constructed and expressed in Escherichia coli. The purified CTB/SS fusion protein polymerized into a biologically active pentamer required for CTB binding to the GM1 ganglioside receptor. Immunization with the CTB/SS protein induced specific immunity against CTB and SS in mice. The serum growth hormone of the CTB/SS-treated mice increased by 29 % (P < 0.05) compared with the control. The results indicated that the CTB/SS fusion protein was effective in inducing immune response against SS as well as elevating the growth hormone level.
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou, China. yingxianaa@163.com
authors.forename: Bingbing
authors.initials: B
authors.lastname: Wu
authors.forename: Taoyan
authors.initials: T
authors.lastname: Yuan
authors.forename: Ruili
authors.initials: R
authors.lastname: Qi
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
authors.forename: Dong
authors.initials: D
authors.lastname: Niu
authors.forename: Weifen
authors.initials: W
authors.lastname: Li
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000235:genetics; D000818:Animals; D002772:Cholera Toxin / Q000008:administration & dosage* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D013006:Growth Hormone / Q000097:blood*; D007114:Immunization / Q000379:methods; D051379:Mice; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D013004:Somatostatin / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D013004:Somatostatin; D013006:Growth Hormone; D002772:Cholera Toxin