title: Pre-ischemic treadmill training for prevention of ischemic brain injury via regulation of glutamate and its transporter GLT-1.
abstract: Pre-ischemic treadmill training exerts cerebral protection in the prevention of cerebral ischemia by alleviating neurotoxicity induced by excessive glutamate release following ischemic stroke. However, the underlying mechanism of this process remains unclear. Cerebral ischemia-reperfusion injury was observed in a rat model after 2 weeks of pre-ischemic treadmill training. Cerebrospinal fluid was collected using the microdialysis sampling method, and the concentration of glutamate was determined every 40 min from the beginning of ischemia to 4 h after reperfusion with high-performance liquid chromatography (HPLC)-fluorescence detection. At 3, 12, 24, and 48 h after ischemia, the expression of the glutamate transporter-1 (GLT-1) protein in brain tissues was determined by Western blot respectively. The effect of pre-ischemic treadmill training on glutamate concentration and GLT-1 expression after cerebral ischemia in rats along with changes in neurobehavioral score and cerebral infarct volume after 24 h ischemia yields critical information necessary to understand the protection mechanism exhibited by pre-ischemic treadmill training. The results demonstrated that pre-ischemic treadmill training up-regulates GLT-1 expression, decreases extracellular glutamate concentration, reduces cerebral infarct volume, and improves neurobehavioral score. Pre-ischemic treadmill training is likely to induce neuroprotection after cerebral ischemia by regulating GLT-1 expression, which results in re-uptake of excessive glutamate.
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.|State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.|State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.
authors.forename: Zhijie
authors.identifier: 0000-0002-3237-4539
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.|Department of Sports Medicine and Rehabilitation, Medical College of Fudan University, Shanghai 200032, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.|State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.|Department of Sports Medicine and Rehabilitation, Medical College of Fudan University, Shanghai 200032, China.
authors.forename: Yongshan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: The Third Teaching Hospital of Xinxiang Medical University, Xinxiang 453003, China.
authors.forename: Xiaolou
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Mingfen
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.
authors.forename: Jingchun
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China.|State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.|Department of Sports Medicine and Rehabilitation, Medical College of Fudan University, Shanghai 200032, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jia
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001930:Brain Injuries / Q000378:metabolism / Q000517:prevention & control*; D002545:Brain Ischemia / Q000378:metabolism / Q000517:prevention & control*; D027342:Excitatory Amino Acid Transporter 2 / Q000378:metabolism*; D005081:Exercise Therapy / Q000295:instrumentation / Q000379:methods*; D005971:Glutamates / Q000378:metabolism*; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D017551:Microdialysis; D018696:Neuroprotective Agents; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D027342:Excitatory Amino Acid Transporter 2; D005971:Glutamates; D018696:Neuroprotective Agents; C493395:Slc1a2 protein, rat