title: FAM20C plays an essential role in the formation of murine teeth.
abstract: FAM20C is highly expressed in bone and tooth. Previously, we showed that Fam20C conditional knock-out (KO) mice manifest hypophosphatemic rickets, which highlights the crucial roles of this molecule in promoting bone formation and mediating phosphate homeostasis. In this study, we characterized the dentin, enamel, and cementum of Sox2-Cre-mediated Fam20C KO mice. The KO mice exhibited small malformed teeth, severe enamel defects, very thin dentin, less cementum than normal, and overall hypomineralization in the dental mineralized tissues. In situ hybridization and immunohistochemistry analyses revealed remarkable down-regulation of dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein in odontoblasts, along with a sharply reduced expression of ameloblastin and amelotin in ameloblasts. Collectively, these data indicate that FAM20C is essential to the differentiation and mineralization of dental tissues through the regulation of molecules critical to the differentiation of tooth-formative cells.
authors.affiliation: Department of Biomedical Sciences, Texas A&M Health Science Center Baylor College of Dentistry, Dallas, Texas 75246, USA.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Wang
authors.forename: Suzhen
authors.initials: S
authors.lastname: Wang
authors.forename: Yongbo
authors.initials: Y
authors.lastname: Lu
authors.forename: Monica P
authors.initials: MP
authors.lastname: Gibson
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Baozhi
authors.initials: B
authors.lastname: Yuan
authors.forename: Jian Q
authors.initials: JQ
authors.lastname: Feng
authors.forename: Chunlin
authors.initials: C
authors.lastname: Qin
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D003746:Dental Enamel Proteins / Q000096:biosynthesis / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006706:Homeostasis / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D009804:Odontoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis / Q000502:physiology; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D012795:Sialoglycoproteins / Q000096:biosynthesis / Q000235:genetics; D014070:Tooth / Q000166:cytology / Q000196:embryology*; D014074:Tooth Calcification / Q000502:physiology*; D000099311:Dentin Sialophosphoprotein
chemical_list: D002135:Calcium-Binding Proteins; D003746:Dental Enamel Proteins; D016326:Extracellular Matrix Proteins; D010710:Phosphates; D010750:Phosphoproteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C490890:Ambn protein, mouse; C513308:Dmp1 protein, mouse; C509696:FAM20C protein, mouse