title: Crystallization and preliminary crystallographic studies of FoxE from Rhodobacter ferrooxidans SW2, an Fe(II) oxidoreductase involved in photoferrotrophy.
abstract: FoxE is a protein encoded by the foxEYZ operon of Rhodobacter ferrooxidans SW2 that is involved in Fe(II)-based anoxygenic photosynthesis (`photoferrotrophy'). It is thought to reside in the periplasm, where it stimulates light-dependent Fe(II) oxidation. It contains 259 residues, including two haem c-binding motifs. As no three-dimensional model is available and there is no structure with a similar sequence, crystals of FoxE were produced. They diffracted to 2.44 Å resolution using synchrotron radiation at the Fe edge. The phase problem was solved by SAD using SHELXC/D/E and the experimental maps confirmed the presence of two haems per molecule.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2781-901 Oeiras, Portugal.
authors.forename: L
authors.initials: L
authors.lastname: Pereira
authors.forename: I H
authors.initials: IH
authors.lastname: Saraiva
authors.forename: R
authors.initials: R
authors.lastname: Coelho
authors.forename: D K
authors.initials: DK
authors.lastname: Newman
authors.forename: R O
authors.initials: RO
authors.lastname: Louro
authors.forename: C
authors.initials: C
authors.lastname: Frazão
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D010088:Oxidoreductases / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D016992:Rhodobacter / Q000201:enzymology*
chemical_list: D010088:Oxidoreductases