title: Intranasal co-delivery with the mouse zona pellucida 3 and GM-CSF expressing constructs enhances humoral immune responses and contraception in mice.
abstract: Granulocyte-monocyte colony-stimulating factor (GM-CSF) regulates the function of antigen-presenting cells (APCs) and has been broadly used as the adjuvant. Here, we tested whether intranasal delivery of GM-CSF can improve the contraception of mouse zona pellucida 3 (mZP3) DNA vaccine. Our results showed that co-administration of GM-CSF and mZP3 DNA vaccine increased the levels of secretory IgA (sIgA) and IgG antibodies in vaginal washes and serum, respectively. Co-administration enhanced Th2 responses through improving the maturation of dendritic cells. Importantly, GM-CSF significantly reduced the fertility rate and mean litter size induced by mZP3 DNA vaccine alone without interfering the normal follicular development. These data suggest that GM-CSF could be used as adjuvant to develop immunocontraceptive vaccine.
authors.affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China.
authors.forename: X
authors.initials: X
authors.lastname: Ma
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D003269:Contraception, Immunologic / Q000379:methods*; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D004527:Egg Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage*; D006801:Humans; D056724:Immunity, Humoral / Q000187:drug effects / Q000235:genetics; D007074:Immunoglobulin G / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D022642:Vaccines, Contraceptive / Q000008:administration & dosage*; D019444:Vaccines, DNA / Q000008:administration & dosage; D000072216:Zona Pellucida Glycoproteins
chemical_list: D000276:Adjuvants, Immunologic; D004527:Egg Proteins; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D022642:Vaccines, Contraceptive; D019444:Vaccines, DNA; D000072216:Zona Pellucida Glycoproteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor