title: [Comparative study between primary mediastinal B-cell lymphoma and non-mediastinal diffuse large B-cell lymphoma by immunoglobulin gene rearrangement and Epstein-Barr virus infection detection].
abstract: OBJECTIVE
  To investigate the differences between primary mediastinal B-cell lymphoma (PMBCL) and non-mediastinal conventional diffuse large B-cell common lymphoma (DLBCL) in immunoglobulin gene rearrangement and EB virus infections.
  
  
  METHODS
  Twenty cases of PMBCL and 30 cases of non-mediastinal DLBCL were collected from September, 2000 to May, 2011. Pathological data were retrospectively analysed. Immunoglobulin heavy chain and light chain gene rearrangements and EBER in-situ hybridization were performed.
  
  
  RESULTS
  Six of 20 cases of PMBCL showed monoclonal gene rearrangement, all of which were weakly detected. Twenty-seven of 30 cases of ordinary diffuse large B-cell lymphoma showed monoclonal gene rearrangement, which were strongly detected (90.0%). Only 1 of 20 cases PMBCL and 2 of 30 cases of DLBCL were positive for EBER in-situ hybridization.
  
  
  CONCLUSIONS
  The detection rate of immunoglobulin gene rearrangement is significantly lower in PMBCL than that of non-mediastinal DLBCL. However, EB virus infection rates are very low in both types of lymphomas.
authors.affiliation: Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100730, China.
authors.forename: Ding-rong
authors.initials: DR
authors.lastname: Zhong
authors.forename: Qing
authors.initials: Q
authors.lastname: Ling
authors.forename: Xiao-hua
authors.initials: XH
authors.lastname: Shi
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Liang
authors.forename: Tong-hua
authors.initials: TH
authors.lastname: Liu
mesh_terms: D000328:Adult; D020031:Epstein-Barr Virus Infections; D005260:Female; D015322:Gene Rearrangement, B-Lymphocyte; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D017403:In Situ Hybridization; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000821:virology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000145:classification / Q000235:genetics / Q000821:virology; D008297:Male; D008479:Mediastinal Neoplasms / Q000235:genetics / Q000821:virology; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D012367:RNA, Viral