title: [Effect of sphingosine 1-phosphate/sphingosine 1-phosphate receptor signal pathway on function of neutrophils].
abstract: The aim of this study was to examine the priming effect of sphingosine 1-phosphate (S1P) on fMLP-activated neutrophils, mainly to detect the neutrophil respiratory burst products, and to investigate the signaling pathway involved in S1P activity. Flow cytometry was used to evaluate the new isolated neutrophil; the superoxide anion output was detected indirectly by cytochrome C reduction in respiratory burst; the dihydro-rhodamine 123 was used to detect the intensity of respiratory burst; the signal transduction pathways of neutrophil respiratory burst were explored by Western blot. The results showed that after pretreated with S1P, the level of superoxide anion released by fMLP-activated neutrophils significantly increased; the Rhodamine 123 mean fluorescence intensity in S1P primed fMLP-activated neutrophils group was significantly higher than that in fMLP treatment group; PI3K and Akt proteins involved in the signal pathway of neutrophil respiratory burst. It is concluded that S1P is a new priming reagent, which primes respiratory burst of fMLP-activated neutrophils; this signal pathway may be that S1P interacts with its receptor, activates PI3K, then activates Akt-transmitting signals through NADPH oxidase, finally results in the respiratory burst.
authors.affiliation: Department of Biology and Medicine, East China Normal University, Shanghai, China.
authors.forename: Zhong-Ying
authors.initials: ZY
authors.lastname: Wang
authors.forename: Ru-Feng
authors.initials: RF
authors.lastname: Xie
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Ya-Na
authors.initials: YN
authors.lastname: Ren
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Yang
authors.forename: Hua-Hua
authors.initials: HH
authors.lastname: Fan
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D008246:Lysophospholipids / Q000378:metabolism*; D019255:NADPH Oxidases / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism* / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D049349:Receptors, Lysosphingolipid / Q000378:metabolism*; D016897:Respiratory Burst; D015398:Signal Transduction; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000378:metabolism; D000081025:Sphingosine-1-Phosphate Receptors; D013481:Superoxides / Q000378:metabolism
chemical_list: D008246:Lysophospholipids; D049349:Receptors, Lysosphingolipid; C477522:S1PR2 protein, human; D000081025:Sphingosine-1-Phosphate Receptors; D013481:Superoxides; C060506:sphingosine 1-phosphate; D019255:NADPH Oxidases; D051057:Proto-Oncogene Proteins c-akt; D013110:Sphingosine