title: Rosette tracker: an open source image analysis tool for automatic quantification of genotype effects.
abstract: Image analysis of Arabidopsis (Arabidopsis thaliana) rosettes is an important nondestructive method for studying plant growth. Some work on automatic rosette measurement using image analysis has been proposed in the past but is generally restricted to be used only in combination with specific high-throughput monitoring systems. We introduce Rosette Tracker, a new open source image analysis tool for evaluation of plant-shoot phenotypes. This tool is not constrained by one specific monitoring system, can be adapted to different low-budget imaging setups, and requires minimal user input. In contrast with previously described monitoring tools, Rosette Tracker allows us to simultaneously quantify plant growth, photosynthesis, and leaf temperature-related parameters through the analysis of visual, chlorophyll fluorescence, and/or thermal infrared time-lapse sequences. Freely available, Rosette Tracker facilitates the rapid understanding of Arabidopsis genotype effects.
authors.affiliation: Department of Telecommunications and Information Processing, Ghent University, 9000 Ghent, Belgium.
authors.forename: Jonas
authors.initials: J
authors.lastname: De Vylder
authors.forename: Filip
authors.initials: F
authors.lastname: Vandenbussche
authors.forename: Yuming
authors.initials: Y
authors.lastname: Hu
authors.forename: Wilfried
authors.initials: W
authors.lastname: Philips
authors.forename: Dominique
authors.initials: D
authors.lastname: Van Der Straeten
mesh_terms: D017360:Arabidopsis / Q000033:anatomy & histology* / Q000235:genetics* / Q000254:growth & development; D001331:Automation; D002138:Calibration; D002734:Chlorophyll / Q000378:metabolism; D005453:Fluorescence; D005838:Genotype; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D018515:Plant Leaves / Q000033:anatomy & histology* / Q000235:genetics* / Q000254:growth & development; D017150:Public Sector; D012984:Software; D013055:Spectrophotometry, Infrared; D013696:Temperature; D059008:Time-Lapse Imaging
chemical_list: D002734:Chlorophyll