title: Enantioselective hydrolysis of epichlorohydrin using whole Aspergillus niger ZJB-09173 cells  in organic solvents.
abstract: The enantioselective hydrolysis of racemic epichlorohydrin for the production of enantiopure (S)-epichlorohydrin using whole cells of Aspergillus niger ZJB-09173 in organic solvents was investigated. Cyclohexane was used as the reaction medium based on the excellent enantioselectivity of epoxide hydrolase from A. niger ZJB- 09173 in cyclohexane. However, cyclohexane had a negative effect on the stability of epoxide hydrolase from A. niger ZJB-09173. In the cyclohexane medium, substrate inhibition, rather than product inhibition of catalysis, was observed in the hydrolysis of racemic epichlorohydrin using A. niger ZJB-09173. The racemic epichlorohydrin concentration was markedly increased by continuous feeding of substrate without significant decline of the yield. Ultimately, 18.5% of (S)-epichlorohydrin with 98 percent enantiomeric excess from 153.6 mM of racemic epichlorohydrin was obtained by the dry cells of A. niger ZJB-09173, which was the highest substrate concentration in the production of enantiopure (S)-epichlorohydrin by epoxide hydrolases using an organic solvent medium among the known reports.
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China.
authors.forename: Huo-Xi
authors.initials: HX
authors.lastname: Jin
authors.forename: Zhong-Ce
authors.initials: ZC
authors.lastname: Hu
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology / Q000378:metabolism*; D003510:Cyclohexanes / Q000737:chemistry; D004811:Epichlorohydrin / Q000737:chemistry / Q000378:metabolism*; D004851:Epoxide Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D012997:Solvents; D013237:Stereoisomerism
chemical_list: D003510:Cyclohexanes; D012997:Solvents; D004811:Epichlorohydrin; C506365:Cyclohexane; D004851:Epoxide Hydrolases