title: Elucidation by NMR solution of neurotensin in small unilamellar vesicle environment: molecular surveys for neurotensin receptor recognition.
abstract: Neurotensin (NT) is a tridecapeptide, hormone in the periphery and neurotransmitter in the brain. We used high-resolution nuclear magnetic resonance (NMR) to resolve the three-dimensional structure of NT in a small unilamellar vesicle (SUV) environment. We demonstrate that if the dynamic of the association-dissociation processes of peptide to SUV binding is rapid enough, structural determination can be obtained by solution NMR experiments. Thus, according to the global dynamic of the system, SUVs seem to be an effective model to mimic biological membranes, especially since the lipid composition can be modified or sterols may be added to closely mimic the biological membranes studied. An animated Interactive 3D Complement (I3DC) is available in Proteopedia at http://proteopedia.org/w/Journal:JBSD:2.
authors.affiliation: Laboratoire de Physique et Biophysique, GESVAB EA 3675, ISVV, Université de Bordeaux 2, 146 Rue Léo Saignat, 33076 Bordeaux Cedex, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Da Costa
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bondon
authors.forename: Olivier
authors.initials: O
authors.lastname: Delalande
authors.forename: Liza
authors.initials: L
authors.lastname: Mouret
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Monti
mesh_terms: D008958:Models, Molecular; D009496:Neurotensin / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D018028:Receptors, Neurotensin / Q000737:chemistry* / Q000378:metabolism; D012996:Solutions; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D018028:Receptors, Neurotensin; D012996:Solutions; D053835:Unilamellar Liposomes; D009496:Neurotensin