title: Anti toxic effect of broccoli extract on stannous dichloride toxicity.
abstract: OBJECTIVE
  Since Technetium-99m ((99m)Tc) has favorable physical and chemical characteristics, it is widely used radioisotope in Nuclear Medicine. However, stannous dichloride (SnCl(2)) has been widely used as a reducing agent in labeling procedure of pharmaceutical with radionuclide, it has been realized that SnCl(2) have genotoxic and cytotoxic effects on biological systems. In previous studies, it has been shown that some herbal extract can reduce genotoxic and cytotoxic effects of SnCl(2). In the present study, it is aimed to evaluate the effect of the broccoli extract on the survival of E. coli ATCC 25922 strain against to toxic effects of SnCl(2).
  
  
  METHODS
  Broccoli was extracted with methanol extraction. HPLC and TLC analysis of broccoli extract were performed. Then antitoxicity and dose response assays were performed on bacterial strain.
  
  
  RESULTS
  The broccoli extract had dose dependent protective effect against SnCl(2) toxic effect on E. coli.
  
  
  CONCLUSIONS
  The consumption of broccoli may alter the stannous dichloride toxicity. Broccoli extract may use as a new protective strategies against the toxic effect of SnCl(2) on patients who were taken (99m)Tc radiopharmaceuticals.
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, Bornova, Izmir-Turkey.
authors.forename: Betul
authors.initials: B
authors.lastname: Cekic
authors.forename: Fazilet Zumrut
authors.initials: FZ
authors.lastname: Biber Muftuler
authors.forename: Ayfer
authors.initials: A
authors.lastname: Yurt Kilcar
authors.forename: Necati
authors.initials: N
authors.lastname: Gunay
authors.forename: Serhan
authors.initials: S
authors.lastname: Sakarya
authors.forename: Perihan
authors.initials: P
authors.lastname: Unak
mesh_terms: D001937:Brassica / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D019275:Radiopharmaceuticals / Q000037:antagonists & inhibitors / Q000633:toxicity*; D013667:Technetium / Q000633:toxicity*; D017971:Tin Compounds / Q000037:antagonists & inhibitors / Q000633:toxicity*
chemical_list: D010936:Plant Extracts; D019275:Radiopharmaceuticals; D017971:Tin Compounds; C023599:stannous chloride; D013667:Technetium