title: Molecular analysis of mutants of the Neurospora adenylosuccinate synthetase locus.
abstract: The ad-8 gene of Neurospora crassa, in addition to being used for the study of purine biology, has been extensively studied as a model for gene structure, mutagenesis and intralocus recombination. Because of this there is an extensive collection of well-characterized N. crassa ad-8 mutants in the Fungal Genetics Stock Center collection. Among these are spontaneous mutants and mutants induced with X-ray, UV or chemical mutagens. The specific lesions in these mutants have been genetically mapped at high resolution. We have sequenced the ad-8 locus from 13 of these mutants and identified the molecular nature of the mutation in each strain. We compare the historical fine-structure map to the DNA and amino acid sequence of each allele. The placement of the individual lesions in the fine-structure map was more accurate at the 5' end of the gene and no mutants were identified in the 3' untranslated region of this gene. We additionally analysed ad-8(+) alleles in 18 N. crassa strains subjected to whole-genome sequence analysis and describe the variability among Neurospora strains and among fungi and other organisms.
authors.affiliation: Fungal Genetics Stock Center, University of Missouri Kansas City, School of Biological Sciences, 5007 Rockhill Road, Kansas City, MO 64113, USA.
authors.forename: A
authors.initials: A
authors.lastname: Wiest
authors.forename: A J
authors.initials: AJ
authors.lastname: McCarthy
authors.forename: R
authors.initials: R
authors.lastname: Schnittker
authors.forename: K
authors.initials: K
authors.lastname: McCluskey
mesh_terms: D000265:Adenylosuccinate Synthase / Q000235:genetics*; D000483:Alleles; D004252:DNA Mutational Analysis; D005656:Fungal Proteins / Q000235:genetics*; D016296:Mutagenesis; D009154:Mutation; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D010802:Phylogeny
chemical_list: D005656:Fungal Proteins; D000265:Adenylosuccinate Synthase