title: Direct trace-elemental analysis of urine samples by laser ablation-inductively coupled plasma mass spectrometry after sample deposition on clinical filter papers.
abstract: Collection of biological fluids on clinical filter papers shows important advantages from a logistic point of view, although analysis of these specimens is far from straightforward. Concerning urine analysis, and particularly when direct trace elemental analysis by laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) is aimed at, several problems arise, such as lack of sensitivity or different distribution of the analytes on the filter paper, rendering obtaining reliable quantitative results quite difficult. In this paper, a novel approach for urine collection is proposed, which circumvents many of these problems. This methodology consists on the use of precut filter paper discs where large amounts of sample can be retained upon a single deposition. This provides higher amounts of the target analytes and, thus, sufficient sensitivity, and allows addition of an adequate internal standard at the clinical lab prior to analysis, therefore making it suitable for a strategy based on unsupervised sample collection and ulterior analysis at referral centers. On the basis of this sampling methodology, an analytical method was developed for the direct determination of several elements in urine (Be, Bi, Cd, Co, Cu, Ni, Sb, Sn, Tl, Pb, and V) at the low μg L(-1) level by means of LA-ICPMS. The method developed provides good results in terms of accuracy and LODs (≤1 μg L(-1) for most of the analytes tested), with a precision in the range of 15%, fit-for-purpose for clinical control analysis.
authors.affiliation: Centro Universitario de la Defensa-Academia General Militar de Zaragoza, Carretera de Huesca s/n, 50090, Zaragoza, Spain.
authors.forename: Maite
authors.initials: M
authors.lastname: Aramendía
authors.forename: Luis
authors.initials: L
authors.lastname: Rello
authors.forename: Frank
authors.initials: F
authors.lastname: Vanhaecke
authors.forename: Martín
authors.initials: M
authors.lastname: Resano
mesh_terms: D005374:Filtration / Q000295:instrumentation; D006801:Humans; D053685:Laser Therapy / Q000295:instrumentation; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000295:instrumentation*; D010209:Paper; D014131:Trace Elements / Q000302:isolation & purification* / Q000652:urine*
chemical_list: D014131:Trace Elements