title: Self-assembling peptide-enriched electrospun polycaprolactone scaffolds promote the h-osteoblast adhesion and modulate differentiation-associated gene expression.
abstract: Electrospun polycaprolactone (PCL) is able to support the adhesion and growth of h-osteoblasts and to delay their degradation rate to a greater extent with respect to other polyesters. The drawbacks linked to its employment in regenerative medicine arise from its hydrophobic nature and the lack of biochemical signals linked to it. This work reports on the attempt to add five different self-assembling (SA) peptides to PCL solutions before electrospinning. The hybrid scaffolds obtained had regular fibers (SEM analysis) whose diameters were similar to those of the extracellular matrix, more stable hydrophilic (contact angle measurement) surfaces, and an amorphous phase constrained by peptides (DSC analysis). They appeared to have a notable capacity to promote the h-osteoblast adhesion and differentiation process by increasing the gene expression of alkaline phosphatase, bone sialoprotein, and osteopontin. Adding an Arg-Gly-Asp (RGD) motif to a self-assembling sequence was found to enhance cell adhesion, while the same motif condensed with a scrambled sequence did not, indicating that there is a cooperative effect between RGD and 3D architecture created by the self-assembling peptides. The study demonstrates that self-assembling peptide scaffolds are still able to promote beneficial effects on h-osteoblasts even after they have been included in electrospun polycaprolactone. The possibility of linking biochemical messages to self-assembling peptides could lead the way to a 3D decoration of fibrous scaffolds.
authors.affiliation: Department of Industrial Engineering, University of Padua, Via Marzolo 9, 35131, Padua, Italy. roberta.danesin@unipd.it
authors.forename: Roberta
authors.initials: R
authors.lastname: Danesin
authors.forename: Paola
authors.initials: P
authors.lastname: Brun
authors.forename: Martina
authors.initials: M
authors.lastname: Roso
authors.forename: Florian
authors.initials: F
authors.lastname: Delaunay
authors.forename: Valérie
authors.initials: V
authors.lastname: Samouillan
authors.forename: Katya
authors.initials: K
authors.lastname: Brunelli
authors.forename: Giovanna
authors.initials: G
authors.lastname: Iucci
authors.forename: Francesca
authors.initials: F
authors.lastname: Ghezzo
authors.forename: Michele
authors.initials: M
authors.lastname: Modesti
authors.forename: Ignazio
authors.initials: I
authors.lastname: Castagliuolo
authors.forename: Monica
authors.initials: M
authors.lastname: Dettin
mesh_terms: D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D009842:Oligopeptides / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D009842:Oligopeptides; D010455:Peptides; D011091:Polyesters; C016240:polycaprolactone; C047981:arginyl-glycyl-aspartic acid