title: Rotavirus genotyping in gastroenteritis cases of an infantile population from Western Brazilian Amazonia.
abstract: BACKGROUND
  During the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the Western Brazilian Amazon.
  
  
  METHODS
  Molecular characterization of the G and P genotypes was performed using RT-PCR and electropherotyping analysis by polyacrylamide gel electrophoresis.
  
  
  RESULTS
  A total of 59 samples were confirmed as group A rotavirus. A long electrophoretic profile was exhibited by the G1P[8], G3P[8], and G4P[8] genotypes. The G1P[8] genotype was found in greater proportion. The short electropherotype was exhibited only by G2 genotype strains.
  
  
  CONCLUSIONS
  The proportion of the rotavirus genotypes observed was not different from that in other areas of Brazil. This study is the first genotyping of rotavirus in the Western Brazilian Amazon.
authors.affiliation: Setor de Microbiologia, Centro de Pesquisa em Medicina Tropical, Porto Velho, RO, Brasil.
authors.forename: Maria Sandra Moura
authors.initials: MS
authors.lastname: Costa
authors.forename: Paulo Afonso
authors.initials: PA
authors.lastname: Nogueira
authors.forename: Gleicienne Félix
authors.initials: GF
authors.lastname: Magalhães
authors.forename: Paula
authors.initials: P
authors.lastname: Taquita
authors.forename: Luis André
authors.initials: LA
authors.lastname: Mariúba
authors.forename: Marilene
authors.initials: M
authors.lastname: Penatti
authors.forename: Patrícia Puccinelli
authors.initials: PP
authors.lastname: Orlandi
mesh_terms: D000208:Acute Disease; D001938:Brazil / Q000453:epidemiology; D002648:Child; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology*; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006801:Humans; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*
chemical_list: D012367:RNA, Viral