title: Perfusion with magnesium sulfate increases sFlt-1 secretion only in the fetal side of placenta of women with preeclampsia.
abstract: OBJECTIVE
  To examine the effect of magnesium sulfate (MgSO(4)) on sFlt (soluble fms-like tyrosine kinase)-1 in the fetal and maternal compartments of normotensive and preeclamptic placentas.
  
  
  METHODS
  Cotyledons of term normotensive and preeclamptic placentas were dually perfused for six hours, with control medium and MgSO(4) (6-7 mg %) in the maternal reservoir. Perfusate sFlt-1 concentrations were measured.
  
  
  RESULTS
  Median sFlt-1 concentration was higher in the maternal than in the fetal side in both groups and perfusion media (p < 0.0001). When perfused with control medium, the maternal side median sFlt-1 concentration was higher in the preeclampsia than in the control group (p < 0.0001). After perfusion with MgSO(4), the median maternal and fetal sides perfusate sFlt-1 concentration were higher in the preeclampsia than in the control group (p < 0.0001). In comparison to perfusion with control medium, the median sFlt-1 concentration of normal pregnant women decreased in the fetal and increased in the maternal side. In the preeclampsia group, only median fetal side sFlt-1 concentration increased.
  
  
  CONCLUSIONS
  In contrast to normal pregnant women, perfusion with MgSO(4) of preeclamptic placentas did not increase their sFlt-1 concentration. This may indicate that MgSO(4) role may be limited to its anti-eclamptic and does not affect the anti-angiogenic state associated with preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Soroka University Medical Center, Faculty of Health Sciences Ben-Gurion University of Negev, Beer Sheva, Israel.
authors.forename: Tamar
authors.initials: T
authors.lastname: Eshkoli
authors.forename: Gershon
authors.initials: G
authors.lastname: Holcberg
authors.forename: Bella
authors.initials: B
authors.lastname: Bronfenmacher
authors.forename: Alaa
authors.initials: A
authors.lastname: Amash
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Huleihel
authors.forename: Offer
authors.initials: O
authors.lastname: Erez
mesh_terms: D000328:Adult; D000927:Anticonvulsants / Q000494:pharmacology* / Q000627:therapeutic use; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008278:Magnesium Sulfate / Q000494:pharmacology* / Q000627:therapeutic use; D010920:Placenta / Q000187:drug effects* / Q000378:metabolism; D011225:Pre-Eclampsia / Q000188:drug therapy*; D011247:Pregnancy; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000927:Anticonvulsants; D008278:Magnesium Sulfate; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1