title: [Screening of proteins regulated by CCDC19 gene in nasopharyngeal carcinoma using proteomics technology].
abstract: OBJECTIVE
  To screen the proteins under regulation by the candidate tumor suppressor gene CCDC19 using proteomics technology in nasopharyngeal carcinoma (NPC).
  
  
  METHODS
  The cellular proteins were extracted from 3D8 NPC cells with CCDC19 overexpression and the control C6 NPC cells. Two-dimensional (2D) gel electrophoresis was employed to compare the protein expression profiles between these two cells, and the differential proteins were identified using peptide mass fingerprinting and database searching. Real-time PCR and Western blotting were used to validate the expression levels of the differential proteins.
  
  
  RESULTS
  Matrix-assisted laser desorption/time of flight showed that 3 differential proteins, namely FASN, CTSD and PGK1, were down-regulated by -3.28, -1.64, and -6.97 folds, respectively, which were confirmed by real-time PCR and Western blotting.
  
  
  CONCLUSIONS
  FASN, CTSD and PGK1 are probably the target proteins regulated by CCDC19 in NPC.
authors.affiliation: Cancer Research Institute, Southern Medical University, Guangzhou, China. fangweiyi1975@yahoo.com.cn
authors.forename: Weiyi
authors.initials: W
authors.lastname: Fang
authors.forename: Qingping
authors.initials: Q
authors.lastname: Jiang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D012313:RNA / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C121996:CFAP45 protein, human; D003598:Cytoskeletal Proteins; D011506:Proteins; D012313:RNA