title: Direct characterization of native chemical ligation of peptides on silicon nanowires.
abstract: We describe the site-specific and chemoselective immobilization of peptides on hydrogen-terminated silicon nanowires (SiNWs) using native chemical ligation (NCL) (i.e., the reaction of a thioester group with a cysteine moiety to give a stable amide bond). The SiNWs investigated in this work were grown via a vapor-liquid-solid mechanism and functionalized with a thioester moiety. The immobilization of the peptides on the SiNWs was demonstrated by synthesizing peptides with an N-terminal cysteine residue and labeled with tetramethylrhodamine or trifluoromethyl groups that were detected by fluorescence and X-ray photoelectron spectroscopy, respectively. The peptides labeled with tetramethylrhodamine or trifluoromethyl groups for fluorescence or X-ray photoelectron spectroscopy (XPS) detection studies were synthesized with an N-terminal cysteine residue. N-Terminal seryl peptides and carboxy-terminated SiNWs were used as controls to demonstrate the chemoselectivity of the peptide immobilization.
authors.affiliation: IBL, UMR CNRS 8161, 1 rue du professeur Calmette, Lille, France.
authors.forename: Nabil
authors.initials: N
authors.lastname: Dendane
authors.forename: Oleg
authors.initials: O
authors.lastname: Melnyk
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Bruno
authors.initials: B
authors.lastname: Grandidier
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: Didier
authors.initials: D
authors.lastname: Stiévenard
authors.forename: Yannick
authors.initials: Y
authors.lastname: Coffinier
mesh_terms: D015394:Molecular Structure; D053770:Nanowires / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*
chemical_list: D010455:Peptides; D012825:Silicon