title: Production and crystallization of α-phosphoglucomutase from Lactococcus lactis.
abstract: α-Phosphoglucomutase (α-PGM) is an enzyme that is essential for the growth of Lactococcus lactis. The enzyme links bacterial anabolism with sugar utilization through glycolysis by catalyzing the reversible interconversion of glucose 6-phosphate and α-glucose 1-phosphate. The gene encoding α-PGM was cloned and overexpressed in L. lactis. The purified protein was functionally active and was crystallized with ammonium sulfate as a precipitant using vapour-diffusion and seeding techniques. Optimized crystals diffracted to 1.5 Å resolution at a synchrotron source.
authors.affiliation: Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa (ITQB-UNL), Avenida da República, 2780-157 Oeiras, Portugal.
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Nogly
authors.forename: Rute
authors.initials: R
authors.lastname: Castro
authors.forename: Matteo
authors.initials: M
authors.lastname: de Rosa
authors.forename: Ana Rute
authors.initials: AR
authors.lastname: Neves
authors.forename: Helena
authors.initials: H
authors.lastname: Santos
authors.forename: Margarida
authors.initials: M
authors.lastname: Archer
mesh_terms: D003460:Crystallization; D013294:Lactococcus lactis / Q000201:enzymology*; D010733:Phosphoglucomutase / Q000737:chemistry*
chemical_list: D010733:Phosphoglucomutase