title: IL-8 release from human neutrophils cultured with pro-haptenic chemical sensitizers.
abstract: Cytokine release from dendritic cells in vitro is a useful marker to discriminate between sensitizing and irritant haptenic chemicals. Unfortunately, pro-haptens, which gain reactivity following metabolic/auto activation, yield negative results. To overcome this, we exposed human neutrophils and THP-1 cells to haptens/pro-haptens and measured IL-8 release. Haptenic compounds stimulated IL-8 release in neutrophils and THP-1 cells. In contrast, the pro-haptens eugenol, isoeugenol, and 2-aminophenol stimulated high levels of IL-8 release from neutrophils alone. Neutrophil cytokine release was reduced when glutathione was added. Cyp1A1/1B1/3A4 were not detectable in THP-1 cells or neutrophils; however, neutrophils expressed high levels of myeloperoxidase.
authors.affiliation: MRC Centre for Drug Safety Science, Department of Pharmacology,  University of Liverpool , Sherrington Building, Ashton Street, Liverpool L69 3GE, England.
authors.forename: Eva
authors.initials: E
authors.lastname: Kiorpelidou
authors.forename: Brian
authors.initials: B
authors.lastname: Foster
authors.forename: John
authors.initials: J
authors.lastname: Farrell
authors.forename: Monday O
authors.initials: MO
authors.lastname: Ogese
authors.forename: Lee
authors.initials: L
authors.lastname: Faulkner
authors.forename: Chris E
authors.initials: CE
authors.lastname: Goldring
authors.forename: B Kevin
authors.initials: BK
authors.lastname: Park
authors.forename: Dean J
authors.initials: DJ
authors.lastname: Naisbitt
mesh_terms: D000627:Aminophenols / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D005054:Eugenol / Q000031:analogs & derivatives* / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*
chemical_list: D000627:Aminophenols; D006241:Haptens; D016209:Interleukin-8; C027667:2-aminophenol; D005054:Eugenol; C036643:isoeugenol