title: A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus.
abstract: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67×10(0)copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were found to be positive by RT-LAMP assay, whereas 39 were positive by real-time RT-PCR. Taken together, the BVDV specific RT-LAMP method is highly specific and sensitive and can be used as a rapid and direct diagnostic assay for testing clinical samples.
authors.affiliation: Department of Biotechnology, Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi 530001, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Fan
authors.forename: Zhixun
authors.initials: Z
authors.lastname: Xie
authors.forename: Liji
authors.initials: L
authors.lastname: Xie
authors.forename: Jiabo
authors.initials: J
authors.lastname: Liu
authors.forename: Yaoshan
authors.initials: Y
authors.lastname: Pang
authors.forename: Xianwen
authors.initials: X
authors.lastname: Deng
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Xie
authors.forename: Yi
authors.initials: Y
authors.lastname: Peng
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000821:virology*; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000821:virology; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D018182:Pestivirus Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine / Q000379:methods*; D014773:Virology / Q000379:methods
chemical_list: D012367:RNA, Viral