title: Preliminary observations of MHC class I A region polymorphism in three populations of Chinese-origin rhesus macaques.
abstract: Rhesus macaques are an animal model for the study of a variety of human diseases. The Chinese rhesus macaques have been widely used in biomedical research in recent years. However, the polymorphism of major histocompatibility complex (MHC) class I A region among different local populations of Chinese rhesus macaques has never been investigated. In this study, we identified 46 Mamu-A alleles by cDNA cloning and sequencing on a cohort of 53 Chinese rhesus monkeys including Zhiming, Chuanxi, and Fujian populations, of which 5 were first reported in rhesus monkeys. The frequencies of alleles were identified for each population. The result suggests that the repertoire of allelic variants of MHC class I A region found in different populations of Chinese macaques is largely non-overlapping. The frequencies of alleles and the popular allele are also different for different populations. PCR-SSP experiment further confirms the different frequencies of two alleles, Mamu-A*026:01 and Mamu-A*022:01, in additional 99 Zhiming monkeys and 191 Chuanxi monkeys. Our findings have important practical implications in that the origin of the individuals and the genetic polymorphism of the monkeys need to be considered at the level of local populations for Chinese rhesus monkeys in biomedical research. Further immunogenetic work is needed to investigate the MHC polymorphism among different populations of Chinese rhesus macaques and to reveal the functional implication of such polymorphism and disease outcome correlations.
authors.affiliation: Laboratory Animal Center of the Academy of Military Medical Science, Beijing, 100071, China.
authors.forename: Aixue
authors.initials: A
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Lihua
authors.initials: L
authors.lastname: Sui
authors.forename: Lin
authors.initials: L
authors.lastname: Zeng
authors.forename: Zhaozeng
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China; D005260:Female; D005787:Gene Frequency; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008253:Macaca mulatta / Q000235:genetics* / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D015395:Histocompatibility Antigens Class I