title: Minocycline treatment and bone marrow mononuclear cell transplantation after endothelin-1 induced striatal ischemia.
abstract: We explored whether the modulation of microglia activation with minocycline is beneficial to the therapeutic actions of bone marrow mononuclear cells (BMMCs) transplanted after experimental stroke. Male Wistar adult rats were divided in four experimental groups: ischemic control saline treated (G1, N = 6), ischemic minocycline treated (G2, N = 5), ischemic BMMC treated (G3, N = 5), and ischemic minocycline/BMMC treated (G4, N = 6). There was a significant reduction in the number of ED1+ cells in G3 animals (51.31 ± 2.41, P < 0.05), but this effect was more prominent following concomitant treatment with minocycline (G4 = 29.78 ± 1.56). There was conspicuous neuronal preservation in the brains of G4 animals (87.97 ± 4.27) compared with control group (G1 = 47.61 ± 2.25, P < 0.05). The behavioral tests showed better functional recovery in animals of G2, G3, and G4, compared with G1 and baseline (P < 0.05). The results suggest that a proper modulation of microglia activity may contribute to a more permissive ischemic environment contributing to increased neuroprotection and functional recovery following striatal ischemia.
authors.affiliation: Laboratory of Experimental Neuroprotection and Neuroregeneration, Institute of Biological Sciences, Federal University of Pará-Brazil, Rua Augusto Corrêa S/N, Campus do Guamá, 66075-900, Belém, Pará, Brazil.
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Cardoso
authors.forename: Edna C S
authors.initials: EC
authors.lastname: Franco
authors.forename: Celice C
authors.initials: CC
authors.lastname: de Souza
authors.forename: Michelle C
authors.initials: MC
authors.lastname: da Silva
authors.forename: Amauri
authors.initials: A
authors.lastname: Gouveia
authors.forename: Walace
authors.initials: W
authors.lastname: Gomes-Leal
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D016026:Bone Marrow Transplantation; D002545:Brain Ischemia / Q000139:chemically induced / Q000188:drug therapy / Q000628:therapy*; D002478:Cells, Cultured; D019332:Endothelin-1; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D008911:Minocycline / Q000627:therapeutic use*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D020521:Stroke / Q000139:chemically induced / Q000188:drug therapy / Q000628:therapy*
chemical_list: D019332:Endothelin-1; D018696:Neuroprotective Agents; D008911:Minocycline