title: Genotyping of BRCA1, BRCA2, p53, CDKN2A, MLH1 and MSH2 genes in a male patient with secondary breast cancer.
abstract: BACKGROUND
  Some tumour suppressor genes (BRCA2) and mismatch repair genes (MSH2, MLH1) are correlated with an increased risk for male breast cancer.
  
  
  METHODS
  Our patient developed secondary breast cancer after the treatment for Hodgkin's disease in childhood. DNA was isolated from the patients' blood and screened for mutations, polymorphisms and variants in BRCA1, BRCA2, p53, CDKN2A, MLH1 and MSH2 genes. We found no mutations but common polymorphisms, and three variants in mismatch repair genes.
  
  
  CONCLUSIONS
  Nucleotide variants c.2006-6T>C and p.G322D in MSH2 might be correlated with male breast cancer.
authors.affiliation: Department of Radiation Oncology, Institute of Oncology, Ljubljana, Slovenia.
authors.forename: Ana Lina
authors.initials: AL
authors.lastname: Vodusek
authors.forename: Srdjan
authors.initials: S
authors.lastname: Novakovic
authors.forename: Vida
authors.initials: V
authors.lastname: Stegel
authors.forename: Berta
authors.initials: B
authors.lastname: Jereb