title: Macrophage migration inhibitory factor covalently complexed with phenethyl isothiocyanate.
abstract: Macrophage migration inhibitory factor is irreversibly inhibited via covalent modification by phenethyl isothiocyanate, a naturally occurring compound with anti-inflammatory and anticancer properties. The structure of the modified protein obtained from X-ray diffraction data to 1.64 Å resolution is presented. The inhibitor sits within a deep hydrophobic pocket between subunits of the homotrimer and is highly ordered. The secondary structure of macrophage migratory inhibitory factor is unchanged by this modification, but there are significant rearrangements, including of the side-chain position of Tyr37 and the main chain of residues 31-34. These changes may explain the decreased binding of the modified protein to the receptor CD74. Together with the pocket, the areas of conformational change define specific targets for the design of more selective and potent inhibitors as potential therapeutics.
authors.affiliation: School of Pharmacy, University of Otago, PO Box 56, Dunedin 9054, New Zealand.
authors.forename: Joel D A
authors.initials: JD
authors.lastname: Tyndall
authors.forename: Hongqi
authors.initials: H
authors.lastname: Lue
authors.forename: Malcolm T
authors.initials: MT
authors.lastname: Rutledge
authors.forename: Jurgen
authors.initials: J
authors.lastname: Bernhagen
authors.forename: Mark B
authors.initials: MB
authors.lastname: Hampton
authors.forename: Sigurd M
authors.initials: SM
authors.lastname: Wilbanks
mesh_terms: D018360:Crystallography, X-Ray; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D017879:Isothiocyanates / Q000737:chemistry* / Q000378:metabolism; D008263:Macrophage Migration-Inhibitory Factors / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D011485:Protein Binding; D017510:Protein Folding; D054730:Protein Interaction Domains and Motifs
chemical_list: D017879:Isothiocyanates; D008263:Macrophage Migration-Inhibitory Factors; C058305:phenethyl isothiocyanate; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human