title: In vitro infection of human umbilical cord blood CD34+ hematopoietic progenitor cells by HIV-1 CRF07_BC enveloped pseudovirus.
abstract: To determine whether CRF07_BC, one of the most predominant strains that accounts for one third HIV-1 prevalence in China, has the ability to infect hematopoietic progenitor cells (HPCs), human Umbilical Cord Blood (UCB) derived CD34+ HPCs isolated with high purity were infected by HIV-1 pseudotyped with CRF07_BC envelope. After HIV-1 infection, ~0.86% CD34+ HPCs were co-stained for CD34 and intracellular HIV Gag. HIV p24 antigen was detectable and reached maximal release between day 2-4 after HIV-1 infection. The data of nested Alu-LTR PCR proved the integration of HIV-1 genome into the host genome occurred in HIV-1-infected HPCs. These data demonstrated that the envelope of CRF07_BC from China has the capability of resulting in infection to CD34+ HPCs, which may serve as a mechanism for long-term latency of HIV-1 infection in vivo.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Qiu
authors.forename: Liangzhu
authors.initials: L
authors.lastname: Li
authors.forename: Aiping
authors.initials: A
authors.lastname: Liu
authors.forename: Mingzhe
authors.initials: M
authors.lastname: Zhou
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Han
authors.forename: Chenli
authors.initials: C
authors.lastname: Qiu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Jianqing
authors.initials: J
authors.lastname: Xu
authors.forename: Huanzhang
authors.initials: H
authors.lastname: Zhu
mesh_terms: D018952:Antigens, CD34 / Q000276:immunology*; D001483:Base Sequence; D002478:Cells, Cultured; D002681:China; D015342:DNA Probes; D004198:Disease Susceptibility / Q000276:immunology; D005260:Female; D005312:Fetal Blood / Q000276:immunology* / Q000821:virology; D005838:Genotype; D015699:HIV Envelope Protein gp120 / Q000276:immunology* / Q000378:metabolism; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification; D006412:Hematopoietic Stem Cells / Q000276:immunology* / Q000821:virology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D015728:Receptors, HIV / Q000276:immunology*; D014779:Virus Replication
chemical_list: D018952:Antigens, CD34; D015342:DNA Probes; D015699:HIV Envelope Protein gp120; D015728:Receptors, HIV