title: Development of microsatellite markers for Anadenanthera colubrina var. cebil (Fabaceae), a native tree from South America.
abstract: OBJECTIVE
  Microsatellite primers were developed in the native legume tree Anadenanthera colubrina var. cebil to study the genetic diversity and genetic structure in natural populations in Argentina.
  
  
  RESULTS
  Nine microsatellite markers were identified using a genomic library enriched for tandemly repeated motifs, eight of which markers were polymorphic. The polymorphism of these markers was assessed by investigating 20 individuals for fragment polymorphism; three to 13 alleles were observed for each locus. Observed and expected heterozygosities ranged from 0.300 to 1.000 and from 0.463 to 0.900, respectively.
  
  
  CONCLUSIONS
  These results confirm that these primers will be useful for investigating the genetic diversity and genetic structure of natural populations of A. colubrina var. cebil in future studies.
authors.affiliation: Departamento de Genética, Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Posadas 3300 Misiones, Argentina. ebarran@fceqyn.unam.edu.ar
authors.forename: María E
authors.initials: ME
authors.lastname: Barrandeguy
authors.forename: Kathleen
authors.initials: K
authors.lastname: Prinz
authors.forename: María V
authors.initials: MV
authors.lastname: García
authors.forename: Reiner
authors.initials: R
authors.lastname: Finkeldey
mesh_terms: D001118:Argentina; D017931:DNA Primers / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D014197:Trees / Q000235:genetics*
chemical_list: D017931:DNA Primers