title: Using a cell-based gas biosensor for investigation of adverse effects of acetone vapors in vitro.
abstract: In this study, a cell-based gas biosensor is presented used for the detection and investigation of gaseous organic compounds in air. The response of living human nasal cells (RPMI 2650) and human lung cells (A549) towards the direct exposure of gaseous substances for 10 min is monitored with a multi-parametric sensor system. Changes in the cellular impedance, oxygen consumption rate and acidification rate can be recorded after the exposure and represent the cytotoxicity of the present gas. The sensor is able to notify the presence of acetone in aqueous solution (2%) but in notably lower concentrations in the gas phase (100-333 ppm) within 30-60 min after the end of the gas exposure. Cell viability is not affected by a sequential exposure to humidified synthetic air (60% r.h.) with a flow rate of 300 ml/min and therefore offers the possibility for a continuous air monitoring. In addition, exposure to synthetic air has no influence on the signals of consecutive acetone exposure. The system might be used in the future for the monitoring of ambient air in work spaces.
authors.affiliation: Corporate Research & Technologies, Siemens AG, Otto-Hahn-Ring 6, 81739 Munich, Germany. ulrichbohrn@hotmail.com
authors.forename: Ulrich
authors.initials: U
authors.lastname: Bohrn
authors.forename: Evamaria
authors.initials: E
authors.lastname: Stütz
authors.forename: Maximilian
authors.initials: M
authors.lastname: Fleischer
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Schöning
authors.forename: Patrick
authors.initials: P
authors.lastname: Wagner
mesh_terms: D000096:Acetone / Q000032:analysis* / Q000633:toxicity*; D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005740:Gases / Q000032:analysis / Q000633:toxicity; D006801:Humans; D008168:Lung / Q000187:drug effects* / Q000502:physiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005740:Gases; D000096:Acetone