title: [The role of oxide stress during the pathogenesis of chronic pancreatic injuries induced by chronic high-fat diets in rat].
abstract: OBJECTIVE
  To provide more detailed information on the roles of lipid peroxidation in the pathogenesis of chronic pancreatic injuries in a pre-clinical rat model.
  
  
  METHODS
  Totally 72 rats were divided into 6 groups (12 in each group) Rats in 5 experimental groups (n = 12) were fed with a high-fat diet (1% cholesterol, 10% lard, 0.3% sodium tauroglycocholate, 87.3% standard rodent chow as the control group) for 2, 4, 6, 10 and 16 weeks, respectively. Morphological studies in the pancreas tissue samples from rats were investigated by using various histological methods. Pancreatic stellate cells (PSCs) were identified by immunohistochemical staining for Desmin and α-smooth muscle actin (α-SMA). The expression of the lipid peroxidation was detected by immunostaining for 4-hydroxy-2-nonenal (4-HNE) and thromboxane A2 receptor (TxA2r). The co-localization of α-SMA and 4-HNE or α-SMA and TxA2r in PSCs was also analyzed in this study.
  
  
  RESULTS
  Pancreatic cells with positive staining for Desmin and α-SMA in HFD rats were distributed in a more extensive way when compared to that in the control group. The levels of pancreatic 4-HNE and TxA2r were increased in rats from HFD groups significantly. The co-localization of 4-HNE and TxA2r were also found within activated PSCs in both of groups.
  
  
  CONCLUSIONS
  The results showed that a chronic HFD feeding may increase the lipid peroxidation process and collagen synthesis through a critical signaling pathway of activated PSCs following pancreatic injuries in rats.
authors.affiliation: Department of General Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Ye-qing
authors.initials: YQ
authors.lastname: Cui
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Hai-chen
authors.initials: HC
authors.lastname: Sun
mesh_terms: D000199:Actins / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D003094:Collagen / Q000096:biosynthesis; D003893:Desmin / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D004195:Disease Models, Animal; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D010179:Pancreas / Q000378:metabolism / Q000473:pathology; D010182:Pancreatic Diseases / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D044244:Receptors, Thromboxane A2, Prostaglandin H2 / Q000378:metabolism
chemical_list: D000199:Actins; D000447:Aldehydes; D003094:Collagen; D003893:Desmin; D044244:Receptors, Thromboxane A2, Prostaglandin H2; C498862:Acta2 protein, rat; C027576:4-hydroxy-2-nonenal