title: Polyester cloth-based hybridization array system for identification of enterohemorrhagic Escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157.
abstract: A cloth-based hybridization array system (CHAS) was developed for the identification of foodborne colony isolates of seven priority enterohemorrhagic Escherichia coli (EHEC-7) serogroups targeted by U. S. food inspection programs. Gene sequences associated with intimin; Shiga-like  toxins 1 and 2; and the antigenic markers O26, O45, O103, O111, O121, O145, and O157 were amplified in a multiplex PCR incorporating a digoxigenin label, and detected by hybridization of the PCR products with an array of specific oligonucleotide probes immobilized on a polyester cloth support,  with subsequent immunoenzymatic assay of the captured amplicons. The EHEC-7 CHAS exhibited 100 % inclusivity and 100 % exclusivity characteristics with respect to detection of the various markers among 89 different E. coli strains, with various marker gene profiles and 15 different  strains of non-E. coli bacteria.
authors.affiliation: Ontario Laboratory Network, Canadian Food Inspection Agency, Central Experimental Farm, Ottawa, Canada. burton.blais@inspection.gc.ca
authors.forename: Burton W
authors.initials: BW
authors.lastname: Blais
authors.forename: Martine
authors.initials: M
authors.lastname: Gauthier
authors.forename: Mylènes
authors.initials: M
authors.lastname: Descheênes
authors.forename: George
authors.initials: G
authors.lastname: Huszczynski
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D003257:Consumer Product Safety; D054324:Enterohemorrhagic Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005819:Genetic Markers; D006801:Humans; D046228:Microarray Analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping; D013997:Time Factors; D037521:Virulence Factors / Q000235:genetics
chemical_list: D005819:Genetic Markers; D037521:Virulence Factors