title: The distribution of human papillomavirus in tissues from patients with             head and neck squamous cell carcinoma.
abstract: Several types of HPVs have been shown to be associated with the development of malignant cancers in various head and neck tumors. More information on the HPV prevalence in patients with head and neck squamous cell carcinomas (SCCs) need to be obtained. In this study, formalin-fixed and paraffin-embedded tissues of 93 pathologically diagnosed head and neck SCC patients were collected from Peking University Cancer Hospital. HPV DNA sequences in tumor tissues were screened by a commercial Luminex technique for HPVs and HPV-specific PCR assays. Presence of HPV16/18 oncoprotein in tumor tissues was assessed by immunohistochemistry (IHC) with HPV16/18 E6-specific antibodies. Of the 93 patients, 16 (17.2%) cases were found to be HPV DNA-positive, including 7 HPV18-positive, 8 HPV16-positive and 1 HPV52-positive. IHC assays demonstrated that 31.2% (29/93) tested sections showed positive signals in the tumor cells. The total positive rate of HPV genome and its encoding products in the tested samples was 44.1% (41/93). Further analyses revealed that HPV infections in head and neck SCCs were significantly related with the tumor anatomic sites, showing decreasing tendency from outside (lip cancer) to inside (laryngeal cancer), but had no correlation with pathological, clinical grades and age of the patients. In all, HPV infections are commonly identified in the tumor tissues of patients with head and neck SCCs, in which HPV16 and 18 are the most prevalent HPV genotypes. Direct detection of high-risk HPV oncoprotein by IHC may be a good tool for classifying a tumor as truly HPV-associated.
authors.affiliation: Key Laboratory of Carcinogenesis and Translational Research, Ministry of Education, Head and Neck Surgery Department Peking University Cancer Hospital and Institute, Beijing 100142, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Qi
authors.initials: Q
authors.lastname: Shi
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.forename: Bao-Yun
authors.initials: BY
authors.lastname: Zhang
authors.forename: Cao
authors.initials: C
authors.lastname: Chen
authors.forename: Nai-Song
authors.initials: NS
authors.lastname: Zhang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D004268:DNA-Binding Proteins; D005260:Female; D006258:Head and Neck Neoplasms / Q000150:complications / Q000821:virology*; D052162:Human papillomavirus 16 / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000276:immunology; D030361:Papillomavirus Infections / Q000150:complications*; D016612:Paraffin Embedding; D012097:Repressor Proteins / Q000276:immunology; D017422:Sequence Analysis, DNA; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C068651:E6 protein, Human papillomavirus type 16; C052603:E6 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins