title: Effects of transmyocardial jet revascularization with chitosan hydrogel on channel patency and angiogenesis in canine infarcted hearts.
abstract: The purpose of this study was to investigate whether transmyocardial jet revascularization (TMJR) with chitosan scaffolds retains channel patency and enhances angiogenesis after acute myocardial infarction (AMI) in a canine model. A total of 32 canines were randomly divided into four groups: myocardial infarction (MI), normal saline (NS), chitosan hydrogel (CH), and chitosan plus growth factor (CH + GF) groups. TMJR was performed surgically using a needle-free injector from the epicardium of canines in the NS, CH, and CH + GF groups; channels were filled with NS, CH, and CH + GF, respectively. After 6 weeks, the patency of the channels and angiogenesis around the channels were assessed by hematoxylin-eosin staining, immunohistochemistry, and Masson's trichrome staining. Results suggest that the channels in the CH and CH + GF groups may retain patency with luminal endothelization. Moreover, the vessel densities of the NS, CH, and CH + GF groups were significantly higher than that of the MI group, and that of the CH + GF group was the highest (p < 0.05). This study suggests that TMJR with chitosan scaffolds may help retain transmyocardial channel patency and enhance angiogenesis after AMI in canines.
authors.affiliation: Department of Cardiology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, People's Republic of China.
authors.forename: Dayan
authors.initials: D
authors.lastname: Zhou
authors.forename: Lu
authors.initials: L
authors.lastname: Xiong
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Zhou
authors.forename: Que
authors.initials: Q
authors.lastname: Zhu
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D048271:Chitosan / Q000494:pharmacology*; D004285:Dogs; D005355:Fibrosis; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D007267:Injections; D009203:Myocardial Infarction / Q000473:pathology* / Q000503:physiopathology*; D009339:Needles; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D013194:Staining and Labeling; D057769:Transmyocardial Laser Revascularization; D014654:Vascular Patency / Q000187:drug effects*
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D048271:Chitosan