title: Recombined sequences between the non-coding control regions of JC and BK viruses found in the urine of a renal transplantation patient.
abstract: Kidney cells are the common host for JC virus (JCV) and BK virus (BKV). Reactivation of JCV and/or BKV in patients after organ transplantation, such as renal transplantation, may cause hemorrhagic cystitis and polyomavirus-associated nephropathy. Furthermore, JCV and BKV may be shed in the urine after reactivation in the kidney. Rearranged as well as archetypal non-coding control regions (NCCRs) of JCV and BKV have been frequently identified in human samples. In this study, three JC/BK recombined NCCR sequences were identified in the urine of a patient who had undergone renal transplantation. They were designated as JC-BK hybrids 1, 2, and 3. The three JC/BK recombinant NCCRs contain up-stream JCV as well as down-stream BKV sequences. Deletions of both JCV and BKV sequences were found in these recombined NCCRs. Recombination of DNA sequences between JCV and BKV may occur during co-infection due to the relatively high homology of the two viral genomes.
authors.affiliation: Clinical Research Center, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Yu-Ching
authors.initials: YC
authors.lastname: Liaw
authors.forename: Cheng-Hsu
authors.initials: CH
authors.lastname: Chen
authors.forename: Kuo-Hsiung
authors.initials: KH
authors.lastname: Shu
authors.forename: Chiung-Yao
authors.initials: CY
authors.lastname: Fang
authors.forename: Wei-Chih
authors.initials: WC
authors.lastname: Ou
authors.forename: Pei-Lain
authors.initials: PL
authors.lastname: Chen
authors.forename: Cheng-Huang
authors.initials: CH
authors.lastname: Shen
authors.forename: Mien-Chun
authors.initials: MC
authors.lastname: Lin
authors.forename: Deching
authors.initials: D
authors.lastname: Chang
authors.forename: Meilin
authors.initials: M
authors.lastname: Wang
mesh_terms: D001739:BK Virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D001483:Base Sequence; D060085:Coinfection / Q000821:virology; D004279:DNA, Viral / Q000652:urine*; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007577:JC Virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D007668:Kidney / Q000473:pathology / Q000821:virology; D016030:Kidney Transplantation; D017354:Point Mutation; D027601:Polyomavirus Infections / Q000652:urine / Q000821:virology; D011995:Recombination, Genetic; D017384:Sequence Deletion; D014412:Tumor Virus Infections / Q000652:urine / Q000821:virology; D020506:Untranslated Regions; D014775:Virus Activation
chemical_list: D004279:DNA, Viral; D020506:Untranslated Regions