title: BDNF-mediated migration of cardiac microvascular endothelial cells is impaired during ageing.
abstract: This study indicates that brain-derived neurotrophic factor (BDNF) can promote young cardiac microvascular endothelial cells (CMECs) to migrate via the activation of the BDNF-TrkB-FL-PI3K/Akt pathway, which may benefit angiogenesis after myocardial infarction (MI). However, the ageing of CMECs led to changes in the expression of receptor Trk isoforms in that among the three isoforms (TrkB-FL, TrkB-T1 and TrkB-T2), only one of its truncated isoforms, TrkB-T1, continued to be expressed, which leads to the dysfunction of its ligand, a decrease in the migration of CMECs and increased injury in ageing hearts. This shift in receptor isoforms in aged CMECs, together with changes in the ageing microenvironment, might predispose ageing hearts to decreased angiogenic potential and increased cardiac pathology.
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, Ji Nan University, Guangzhou, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Cao
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Siyun
authors.initials: S
authors.lastname: Chen
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Yuan
authors.forename: Shaokun
authors.initials: S
authors.lastname: Liu
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Shen
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.forename: Xufeng
authors.initials: X
authors.lastname: Qi
authors.forename: Kenneth K H
authors.initials: KK
authors.lastname: Lee
authors.forename: John Yeuk-Hon
authors.initials: JY
authors.lastname: Chan
authors.forename: Dongqing
authors.initials: D
authors.lastname: Cai
mesh_terms: D000375:Aging; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002465:Cell Movement; D042783:Endothelial Cells / Q000502:physiology*; D005260:Female; D006321:Heart; D009203:Myocardial Infarction; D018919:Neovascularization, Physiologic / Q000502:physiology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D020033:Protein Isoforms / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020033:Protein Isoforms; D019869:Phosphatidylinositol 3-Kinases; D020813:Receptor, trkB; D051057:Proto-Oncogene Proteins c-akt