title: Sensitive analysis of DNA methyltransferase based on a hairpin-shaped DNAzyme.
abstract: The analysis of DNA methylation and MTase (methyltransferase) activity is important in epigenetic study. We have developed a novel strategy for sensitive analysis of MTase activity based on a hairpin shaped DNAzyme; 8-17 DNAzyme amplicon has been adopted and found to be very effective in such analysis.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, PR China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.forename: Heng
authors.initials: H
authors.lastname: Xiao
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Long
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Zhang
authors.forename: Shaoru
authors.initials: S
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.forename: Songmei
authors.initials: S
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
mesh_terms: D019175:DNA Methylation; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism*; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D057075:Enzyme Assays / Q000379:methods*; D012680:Sensitivity and Specificity; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000032:analysis* / Q000378:metabolism
chemical_list: D015342:DNA Probes; D021881:DNA, Catalytic; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)