title: [Renal calculus microflora in urolithiasis and search for agents of control of biofilms formed by uropathogenic bacteria].
abstract: OBJECTIVE
  Study bacterial biofilms in native material (renal calculus) by electron microscopy method and developmeit of biofilm model by isolates in vitro on sterile calculi of various chemical composition.
  
  
  METHODS
  Bacterial spectra of microflora of renal calculus lavages were studied, isolated pure cultures were identified up to species. Comparisons of urine microflora obtained before operation in patients with urolithiasis with microflora of removed renal calculi were carried out.
  
  
  RESULTS
  Urease activity and genes coding pathogenicity factors were detected, and the ability to form biofilms by isolates was studied. Model of formation of biofilms in vitro on sterile renal calculi was developed and candidate agents reducing the biofilm forming ability were tested.
  
  
  CONCLUSIONS
  Uropathogenic microorganisms infecting renal calculi and forming biofilms on them not only support chronic infection by increased resistance to therapy but also facilitate novel lithogenesis.
authors.forename: E R
authors.initials: ER
authors.lastname: Tolordava
authors.forename: I G
authors.initials: IG
authors.lastname: Tiganova
authors.forename: N V
authors.initials: NV
authors.lastname: Alekseeva
authors.forename: T V
authors.initials: TV
authors.lastname: Stepanova
authors.forename: A A
authors.initials: AA
authors.lastname: Terekhov
authors.forename: D K
authors.initials: DK
authors.lastname: Egamberdiev
authors.forename: N S
authors.initials: NS
authors.lastname: Mulabaev
authors.forename: N V
authors.initials: NV
authors.lastname: Shevliagina
authors.forename: L V
authors.initials: LV
authors.lastname: Didenko
authors.forename: Iu M
authors.initials: IuM
authors.lastname: Romanova
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002939:Ciprofloxacin / Q000494:pharmacology; D003470:Culture Media; D006801:Humans; D007668:Kidney / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D007669:Kidney Calculi / Q000382:microbiology* / Q000601:surgery; D007781:Lactoferrin / Q000494:pharmacology; D008096:Lithotripsy; D014510:Urease / Q000032:analysis; D014556:Urine / Q000382:microbiology; D037521:Virulence Factors / Q000032:analysis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D037521:Virulence Factors; D002939:Ciprofloxacin; D007781:Lactoferrin; D014510:Urease