title: Associations of FASN gene polymorphisms with economical traits in Nellore cattle (Bos primigenius indicus).
abstract: The aim of this study was to identify molecular markers to be applied to marker-assisted selection. Three SNPs of the FASN gene were studied. PCR-RFLP was used for genotyping. The SNPs g.17924A>G, g.17860C>T and g.15603A>G all in the FASN gene were genotyped using the enzymes MscI, DdeI and Hae III, respectively. The animals were raised in extensive systems and belong to three lines selected for growth as part of the Selection Program of Zebu and Caracu Breeds, São Paulo, Brazil. Allele and genotype frequencies were compared between selection lines using the Genepop 3.4. Associations between polymorphisms and the traits studied were evaluated using the PROC MIXED procedure of the SAS/STAT 9.1.3. The G and C alleles were the most frequent alleles of the g.15603A>G and g.17860C>T loci, respectively. The g.17924A>G locus showed no polymorphism in the population studied. Allele and genotype frequencies differed significantly between the NeT line and the NeC and NeS lines. The g.15603A>G polymorphism tended to exert an additive effect on rump fat thickness and male yearling height. For g.17860C>T, an additive effect on male yearling height was observed. Genotype combination analysis revealed a significant effect on loin eye area. Although this study provided evidence of an association between the FASN gene and some traits, more detailed analyses are needed to obtain more efficient molecular markers.
authors.affiliation: Faculdade de Ciências Agrárias e Veterinárias, Departamento de Zootecnia, Universidade Estadual Paulista, Via de Acesso Prof.Paulo Donato Castellane, Jaboticabal, SP 14884-900, Brazil. fabiopablos@hotmail.com
authors.forename: Fabio Ricardo Pablos
authors.initials: FR
authors.lastname: de Souza
authors.forename: Milena Gandin
authors.initials: MG
authors.lastname: Chiquitelli
authors.forename: Larissa Fernanda Simielli
authors.initials: LF
authors.lastname: da Fonseca
authors.forename: Diércles Franscisco
authors.initials: DF
authors.lastname: Cardoso
authors.forename: Patrícia Dias
authors.initials: PD
authors.lastname: da Silva Fonseca
authors.forename: Gregório Miguel Ferreira
authors.initials: GM
authors.lastname: de Camargo
authors.forename: Fernanda Maria Monsalves
authors.initials: FM
authors.lastname: Gil
authors.forename: Arione Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Humberto
authors.initials: H
authors.lastname: Tonhati
authors.forename: Maria Eugênia Zerlotti
authors.initials: ME
authors.lastname: Mercadante
authors.forename: Lucia Galvão
authors.initials: LG
authors.lastname: de Albuquerque
mesh_terms: D050154:Adiposity / Q000235:genetics; D000818:Animals; D001699:Biometry; D001835:Body Weight / Q000235:genetics; D001947:Breeding / Q000191:economics; D002417:Cattle / Q000033:anatomy & histology / Q000235:genetics*; D064429:Fatty Acid Synthases / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005819:Genetic Markers; D005838:Genotype; D008297:Male; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D005819:Genetic Markers; D064429:Fatty Acid Synthases