title: [Function of flgK gene in Pectobacterium carotovorum subsp, carotovorum].
abstract: OBJECTIVE
  To investigate functions of flgKpcc gene in Pectobacterium carotovorum subsp. carotovorum (P. c. c).
  
  
  METHODS
  The gene knock-out mutant deltaflgKpcc and complemented strain deltaflgKpcc-KH were generated by biparental mating and their phenotypes including cell morphology, motility, pathogenic factors, and pathogenicity were investigated.
  
  
  RESULTS
  Non-flagellum, cell precipitation in the culture and significantly attenuated motility on 0.3% semisolid medium were observed in deltaflgKpcc compared to Pectobacterium carotovorum subsp. carotovorum S1. In addition, significant decrease in cellulase and protease activity, biofilm formation, and pathogenicity on host plant were found in deltaflgKpcc. While there were no apparent difference in growth rate in vitro, deltaflgKpcc-KH, the complementation strain, restored the phenotype of deltaflgKpcc to the wild type level.
  
  
  CONCLUSIONS
  The gen of flgKpcc not only influences the cell motility, but also pathogenic factors to lead to the decreased pathogenicity in Pectobacterium carotovorum subsp. Carotovorum.
authors.affiliation: College of Plant Protection, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China. 2009102004@njau.edu.cn
authors.forename: Zhongling
authors.initials: Z
authors.lastname: Yang
authors.forename: Yamin
authors.initials: Y
authors.lastname: Deng
authors.forename: Shuo
authors.initials: S
authors.lastname: Du
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Huan
authors.initials: H
authors.lastname: Jiang
authors.forename: Fengquan
authors.initials: F
authors.lastname: Liu
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Fan
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D002480:Cellulase / Q000378:metabolism; D005407:Flagella / Q000378:metabolism; D055785:Gene Knockdown Techniques; D016973:Pectobacterium carotovorum / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D010447:Peptide Hydrolases / Q000378:metabolism; D037521:Virulence Factors / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; C088595:FlgK protein, Bacteria; D037521:Virulence Factors; D002480:Cellulase; D010447:Peptide Hydrolases