title: Proteome analysis of gut and salivary gland proteins of fifth-instar nymph and adults of the sunn pest, Eurygaster integriceps.
abstract: In the digestive system of the sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae), the salivary gland has a key role in extra oral digestion and the gut is the main site for digestion of food. In this study, proteomics was used to study the role of proteins involved in digestion. The amount of feeding on wheat grain by adult insects increased by comparison to fifth-instar nymphs. Proteins of the gut and salivary gland in adults and fifth-instar nymphs were analyzed 1 day after feeding. The proteins related to digestion, metabolism, and defense against toxins were accumulated in the gut of adult insects. Three plant proteins including serpin, dehydroascorbate reductase, and β-amylase were accumulated in guts of adults. In the salivary gland, phospholipase A2 and arginine kinase were increased in adults. Heat shock protein 70 increased in the gut of fifth-instar nymphs. Proteomic analysis revealed that most of changed proteins in digestive system of sunn pest were increased in adults. This study provided more targets derived from gut and salivary gland for pest management.
authors.affiliation: National Institute of Crop Science, Tsukuba, Japan.
authors.forename: Mohammad Saadati
authors.initials: MS
authors.lastname: Bezdi
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Toorchi
authors.forename: Reza Farshbaf
authors.initials: RF
authors.lastname: Pourabad
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
authors.forename: Mohammad-Zaman
authors.initials: MZ
authors.lastname: Nouri
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
mesh_terms: D000818:Animals; D004734:Energy Metabolism / Q000502:physiology; D005247:Feeding Behavior / Q000502:physiology; D041981:Gastrointestinal Tract / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D020062:Heteroptera / Q000235:genetics* / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics; D009758:Nymph; D020543:Proteome / Q000235:genetics*; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism; D015430:Weight Gain / Q000502:physiology
chemical_list: D019476:Insect Proteins; D020543:Proteome; D012471:Salivary Proteins and Peptides