title: Structural and oxidative changes in the kidney of crucian carp induced by silicon-based quantum dots.
abstract: Silicon-based quantum dots were intraperitoneally injected in Carassius auratus gibelio specimens and, over one week, the effects on renal tissue were investigated by following their distribution and histological effects, as well as antioxidative system modifications. After three and seven days, detached epithelial cells from the basal lamina, dilated tubules and debris in the lumen of tubules were observed. At day 7, nephrogenesis was noticed. The reduced glutathione (GSH) concentration decreased in the first three days and started to rise later on. The superoxide dismutase (SOD) activity increased only after one week, whereas catalase (CAT) was up-regulated in a time-dependent manner. The activities of glutathione reductase (GR) and glutathione peroxidise (GPX) decreased dramatically by approximately 50% compared to control, whereas the glutathione-S-transferase (GST) and glucose-6-phosphate dehydrogenase (G6PDH) increased significantly after 3 and 7 days of treatment. Oxidative modifications of proteins and the time-dependent increase of Hsp70 expression were also registered. Our data suggest that silicon-based quantum dots induced oxidative stress followed by structural damages. However, renal tissue is capable of restoring its integrity by nephron development.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Sorina Nicoleta
authors.initials: SN
authors.lastname: Petrache
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Loredana
authors.initials: L
authors.lastname: Stanca
authors.affiliation: Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine, 105 Splaiul Independentei, Bucharest 050097, Romania.
authors.forename: Andreea Iren
authors.initials: AI
authors.lastname: Serban
authors.affiliation: Laser Department, National Institute of Laser, Plasma and Radiation Physics, 409 Atomistilor, Bucharest-Magurele 077125, Romania.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Sima
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Andreia Cristina
authors.initials: AC
authors.lastname: Staicu
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Munteanu
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Marieta
authors.initials: M
authors.lastname: Costache
authors.affiliation: Department of Mathematics, University of Agricultural Sciences and Veterinary Medicine, 59 Marasti Bd., Bucharest 011464, Romania.
authors.forename: Radu
authors.initials: R
authors.lastname: Burlacu
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Otilia
authors.initials: O
authors.lastname: Zarnescu
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
mesh_terms: D000818:Animals; D002347:Carps / Q000254:growth & development / Q000378:metabolism*; D002374:Catalase / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000737:chemistry; D005980:Glutathione Reductase / Q000737:chemistry; D005982:Glutathione Transferase / Q000737:chemistry; D007668:Kidney / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism*; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D045663:Quantum Dots; D012825:Silicon / Q000008:administration & dosage* / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000737:chemistry
chemical_list: D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; D012825:Silicon