title: Citrus psorosis and Mirafiori lettuce big-vein ophiovirus coat proteins localize to the cytoplasm and self interact in vivo.
abstract: Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction. The cytoplasmic localization of this protein was confirmed by confocal microscopy of fluorescent protein (FP)-tagged CP following its expression in either CPsV-infected and healthy Citrus sinensis plants or in Nicotiana benthamiana plants. The same localization was observed for FP-tagged CP of MiLBVV. The CPs of CPsV and MiLBBV can undergo homologous and heterologous interactions as revealed by fluorescent lifetime imaging microscopy and co-immunoprecipitation analysis. A putative leucine zipper motif that is conserved among ophiovirus CP sequences may account for these interactions.
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS (UPR 2357), Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg CEDEX, France.
authors.forename: Eduardo José
authors.initials: EJ
authors.lastname: Peña
authors.forename: Gabriel
authors.initials: G
authors.lastname: Robles Luna
authors.forename: María Cecilia
authors.initials: MC
authors.lastname: Zanek
authors.forename: María Belén
authors.initials: MB
authors.lastname: Borniego
authors.forename: Carina Andrea
authors.initials: CA
authors.lastname: Reyes
authors.forename: Manfred
authors.initials: M
authors.lastname: Heinlein
authors.forename: María Laura
authors.initials: ML
authors.lastname: García
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002957:Citrus / Q000821:virology*; D017124:Conserved Sequence; D003593:Cytoplasm / Q000378:metabolism*; D015870:Gene Expression; D023061:Gene Order; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011485:Protein Binding; D021381:Protein Transport; D012328:RNA Viruses / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment
chemical_list: D036022:Capsid Proteins