title: Genome-wide mapping of nucleosome positions in yeast using high-resolution MNase ChIP-Seq.
abstract: Eukaryotic DNA is packaged into chromatin where nucleosomes form the basic building unit. Knowing the precise positions of nucleosomes is important because they determine the accessibility of underlying regulatory DNA sequences. Here we describe a detailed method to map on a genomic scale the locations of nucleosomes with very high resolution. Micrococcal nuclease (MNase) digestion followed by chromatin immunoprecipitation and facilitated library construction for deep sequencing provides a simple and accurate map of nucleosome positions.
authors.affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA.
authors.forename: Megha
authors.initials: M
authors.lastname: Wal
authors.forename: B Franklin
authors.initials: BF
authors.lastname: Pugh
mesh_terms: D042002:Chromatin Assembly and Disassembly; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D002874:Chromosome Mapping / Q000379:methods; D004271:DNA, Fungal / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D016681:Genome, Fungal; D008836:Micrococcal Nuclease / Q000378:metabolism*; D009707:Nucleosomes / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA / Q000379:methods; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D009707:Nucleosomes; D008836:Micrococcal Nuclease