title: [Response of bone morphogenetic protein-2 and basic fibroblast growth factor in bone marrow stromal cells in ectopic and in situ bone formation].
abstract: OBJECTIVE
  We ascertained the effect of bone morphogenetic protein-2 (BMP-2) and basic fibroblast growth factor (bFGF) by a series of experiments: Proliferation and differentiation of bone marrow stromal cells (BMSCs) in vitro, ectopic and in situ bone formation and loaded porous calcium phosphate cement (CPC) on the repair of bone defects around dental implants.
  
  
  METHODS
  BMSCs from Beagle dogs were cultured in vitro with basic culture medium containing BMP-2, bFGF, and BMP-2+bFGF. Proliferation and differentiation of BMSCs were quantified using methyl thiazolyl tetrazolium (MTT) and alkaline phosphatase (ALP) test. The CPC seeded with BMSCs and BMP-2, bFGF, combined BMP-2 with bFGF were implanted subcutaneously into nude rats in ectopic bone formation, and were implanted into critical-sized bone defects of Beagle dogs in the in situ bone formation. The bone formation was detected by histology examination and quantified using an image analysis system. Polychrome sequential fluorescent labels and fluorescence histological examinations of undecalcified sections were performed post-operatively.
  
  
  RESULTS
  It was determined that BMP-2+bFGF promoted BMSCs statistically significant proliferation and differentiation compared to either BMP-2 or bFGF in vitro. The CPC with BMP-2+bFGF group yielded more bone than those with either BMP-2 or bFGF in ectopic bone formation test. The percentages of newly ectopic formed bone were higher in the BMP-2+bFGF group (48.79% +/- 11.31%) than those in other groups (BMP-2 group, 30.71% +/- 10.85%; bFGF group, 27.33% +/- 9.67%; and the control group, 10.65% +/- 6.05%). Undecalcified showed that new bone was actively formed in the BMP-2+bFGF group after 12 weeks in the in situ bone formation test. The bone mineralization apposition rate (MAR) was better in the BMP-2+bFGF group than in other groups (P<0.01).
  
  
  CONCLUSIONS
  BMP-2 combined with bFGF are more effective than one alone in promoting the formation of new bone.
authors.affiliation: Dept. of Stomatology, Hongqiao Community Medical Service Center in Changning District of Shanghai, Shanghai 200051, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Sulan
authors.initials: S
authors.lastname: You
authors.forename: Luanjun
authors.initials: L
authors.lastname: Tan
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D055396:Bone Morphogenetic Protein 2; D001842:Bone and Bones; D002130:Calcium Phosphates; D002454:Cell Differentiation; D002478:Cells, Cultured; D004285:Dogs; D016222:Fibroblast Growth Factor 2; D059630:Mesenchymal Stem Cells; D051381:Rats
chemical_list: D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D016222:Fibroblast Growth Factor 2; C020243:calcium phosphate