title: Simultaneous quantification of cells and nanomaterials by inductive-coupled plasma techniques.
abstract: We demonstrate that endogenous cellular magnesium levels can be used as an accurate determinant of total cell number by inductively coupled plasma techniques, increasing the throughput and reproducibility of nanoparticle-uptake studies. Uptake of either gold nanoparticles or quantum dots did not affect intracellular concentration of Mg. To demonstrate this technique, we show the decreased uptake of nano-urchins in A549 cells compared with gold nanospheres.
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Albanese
authors.forename: Kim M
authors.initials: KM
authors.lastname: Tsoi
authors.forename: Warren C W
authors.initials: WC
authors.lastname: Chan
mesh_terms: D000818:Animals; D002452:Cell Count; D045744:Cell Line, Tumor; D002477:Cells / Q000378:metabolism*; D016895:Culture Media, Serum-Free; D006046:Gold / Q000378:metabolism; D006801:Humans; D008274:Magnesium / Q000378:metabolism; D053768:Metal Nanoparticles / Q000032:analysis; D051379:Mice; D049329:Nanostructures / Q000032:analysis*; D012815:Signal Processing, Computer-Assisted; D013054:Spectrophotometry, Atomic / Q000379:methods*
chemical_list: D016895:Culture Media, Serum-Free; D006046:Gold; D008274:Magnesium