title: Comparison of cell proliferation and epigenetic modification of gene expression patterns in canine foetal fibroblasts and adipose tissue-derived mesenchymal stem cells.
abstract: OBJECTIVE
  This study compared rate of cell proliferation, viability, cell size, expression patterns of genes related to pluripotency and epigenetic modification between canine foetal fibroblasts (cFF) and canine adipose tissue-derived mesenchymal stem cells (cAd-MSC).
  
  
  METHODS
  Proliferation pattern, cell viability as well as cell size at each passage of cFF and cAd-MSC were measured when cultures reached confluence. In addition, real-time PCR was performed to investigate expression of Dnmt1, HDAC1, OCT4, SOX2, BAX, BCL2 genes with reference to β-actin gene expression as an endogenous control in both cell lines.
  
  
  RESULTS
  cFF and cAd-MSC differed in number of generations, but not in doubling times, at all passages. Mean cell size of cAd-MSC was significantly smaller than that of cFF. Cell viability was significantly lower in cFFs and apoptotic level was significantly lower in cAd-MSC compared to passage-matched cFF. In the expression of genes related to pluripotency and epigenetic modification, level of HDAC1 in cAd-MSC was significantly higher than in cFF, but expression of Dnmt1 did not differ between the two groups. OCT4 and SOX2 were significantly more highly expressed in cAd-MSC compared to cFF.
  
  
  CONCLUSIONS
  cAd-MSC have higher stem-cell potential than cFF in terms of proliferation patterns, epigenetic modification and pluripotency, thus cAd-MSC could be more appropriate than cFF as donors of nuclei in somatic cell nuclear transfer for transgenesis.
authors.affiliation: Department of Theriogenology and Biotechnology, Seoul National University, Seoul, South Korea.
authors.forename: H J
authors.initials: HJ
authors.lastname: Oh
authors.forename: E J
authors.initials: EJ
authors.lastname: Park
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.forename: J W
authors.initials: JW
authors.lastname: Soh
authors.forename: I S
authors.initials: IS
authors.lastname: Kong
authors.forename: S W
authors.initials: SW
authors.lastname: Choi
authors.forename: J C
authors.initials: JC
authors.lastname: Ra
authors.forename: S K
authors.initials: SK
authors.lastname: Kang
authors.forename: B C
authors.initials: BC
authors.lastname: Lee
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D004285:Dogs; D044127:Epigenesis, Genetic / Q000502:physiology*; D005260:Female; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D011247:Pregnancy