title: Relationship between within-host fitness and virulence in the vesicular stomatitis virus: correlation with partial decoupling.
abstract: Given the parasitic nature of viruses, it is sometimes assumed that rates of viral replication and dissemination within hosts (within-host fitness) correlate with virulence. However, there is currently little empirical evidence supporting this principle. To test this, we quantified the fitness and virulence of 21 single- or double-nucleotide mutants of the vesicular stomatitis virus in baby hamster kidney cells (BHK-21). We found that, overall, these two traits correlated positively, but significant outliers were identified. Particularly, a single mutation in the conserved C terminus of the N nucleocapsid (U1323A) had a strongly deleterious fitness effect but did not alter or even slightly increased virulence. We also found a double mutant of the M matrix protein and G glycoprotein (U2617G/A3802G mutant) with high fitness yet low virulence. We further characterized these mutants in primary cultures from mouse brain cells and in vivo and found that their relative fitness values were similar to those observed in BHK-21 cells. The mutations had weak effects on the virus-induced death rate of total brain cells, although they specifically reduced neuron death rates. Furthermore, increased apoptosis levels were detected in neurons infected with the U2617G/A3802G mutant, consistent with its known inability to block interferon secretion. In vivo, this mutant had reduced virulence and, despite its low brain titer, it retained a relatively high fitness value owing to its ability to suppress competitor viruses. Overall, our results are in broad agreement with the notion that viral fitness and virulence should be positively correlated but show that certain mutations can break this association and that the fitness-virulence relationship can depend on complex virus-host and virus-virus interactions.
authors.affiliation: Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, Spain.
authors.forename: Victoria
authors.initials: V
authors.lastname: Furió
authors.forename: Raquel
authors.initials: R
authors.lastname: Garijo
authors.forename: María
authors.initials: M
authors.lastname: Durán
authors.forename: Andrés
authors.initials: A
authors.lastname: Moya
authors.forename: John C
authors.initials: JC
authors.lastname: Bell
authors.forename: Rafael
authors.initials: R
authors.lastname: Sanjuán
mesh_terms: D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000166:cytology / Q000821:virology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D005260:Female; D006023:Glycoproteins / Q000737:chemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D019590:Nucleocapsid Proteins / Q000737:chemistry; D017434:Protein Structure, Tertiary; D054243:Vesicular Stomatitis / Q000821:virology; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence; D014779:Virus Replication
chemical_list: D006023:Glycoproteins; D019590:Nucleocapsid Proteins