title: A simple and practical method that prepares high molecular weight DNA             ladders.
abstract: The purpose of the current study was to report a simple and practical method             to prepare high molecular weight (mw) DNA ladders. The method involves 1,000-4,000-base             pairs (bp) DNA fragments being amplified by polymerase chain reaction (PCR), using             λ DNA as a template. The constructed plasmids are digested by restriction endonucleases             to produce 5-, 6-, 8- and 10-kb DNA fragments, followed by purification and precipitation             with ethanol, and mixed proportionally. The 1,000-4,000-bp DNA fragments were             successfully generated by PCR and 5-, 6-, 8- and 10-kb DNA fragments were obtained             through the digestion of the plasmids. The bands of the prepared high mw DNA ladder             were clear and may aid future molecular biology studies.
authors.affiliation: Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan 453003, PR China.
authors.forename: Jun-He
authors.initials: JH
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Tian-Yun
authors.initials: TY
authors.lastname: Wang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Dong
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D053938:DNA Fragmentation; D004262:DNA Restriction Enzymes / Q000378:metabolism; D008970:Molecular Weight; D010957:Plasmids / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes