title: [Matrix effect and control of immunoassay for environmental samples].
abstract: Immunoassay provides very specific, highly sensitive, rapid, and cost-effective analyses for a variety of environmental contaminants. Since the immunoassay detects the environmental samples without pre-treatment, the interferences caused by various matrixes of environmental samples are a major problem, which can greatly affect the detection results. In this paper, based on the enzyme-linked immunosorbent assay (ELISA) for detection of Microcystin-LR (MC-LR), the effect of many kinds of matrixes on ELISA was systematically analyzed, and the corresponding method to control or eliminate the disbennifit effect was proposed. Finally, an integrated and packaged strategy-using the following buffer system as a diluent, i.e. 0.1 mol x L(-1) pH 7.4 PBS (10 x PBS) containing 1% BSA, 0.5% EDTA and 40 g x L(-1) sodium chloride-was given to control the overall matrix effects of environmental samples on ELISA detection. This strategy is also applicable to other kinds of immunoassays for environmental samples.
authors.affiliation: State Key Joint Laboratory of Environmental Simulation and Pollution Control (ESPC), School of Environment, Tsinghua University, Beijing 100084, China.
authors.forename: Jian-Wu
authors.initials: JW
authors.lastname: Sheng
authors.forename: Miao
authors.initials: M
authors.lastname: He
authors.forename: Han-Chang
authors.initials: HC
authors.lastname: Shi
mesh_terms: D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis* / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000276:immunology
chemical_list: D008387:Marine Toxins; D052998:Microcystins; D014874:Water Pollutants, Chemical; C057862:cyanoginosin LR