title: Magnetic molecularly imprinted nanoparticles based on dendritic-grafting modification for determination of estrogens in plasma samples.
abstract: In order to resolve the low imprinting efficiency of surface molecularly imprinted polymers (MIPs), a dendritic-grafting method introducing more functional groups was proposed to modify the SiO(2)-coated magnetic nanoparticles (SiO(2)-coated MNPs). And then magnetic MIPs (MMIPs) were obtained using 17-ethyl estradiol (EE2) as a pseudo template with dendronized SiO(2)-coated MNPs as the supporter, aiming to avoid residual template leakage and to increase the imprinting efficiency. The resulting MMIPs showed high adsorption capacity, quick binding kinetics and good selectivity for trace estrogens. Meanwhile, MMIPs were used as magnetic dispersive solid-phase extraction (MDSPE) materials coupled with HPLC-UV for the detection of trace estrogens. The amounts of three estrogens which were detected from the plasma samples of pregnant women were 5.28, 5.31 and 4.17 ng mL(-1), and the average recoveries were 87.8%, 93.1% and 90.6% for the spiked samples with RSDs in the range of 1.4-6.3%, respectively. All these results reveal that MMIPs as MDSPE materials has good applicability to selective extraction and separation of trace estrogens from complex samples.
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Wang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Cheng
authors.initials: C
authors.lastname: Xue
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wu
authors.forename: Junli
authors.initials: J
authors.lastname: Hong
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D004967:Estrogens / Q000097:blood* / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008854:Microscopy, Electron; D054802:Molecular Imprinting / Q000295:instrumentation*; D011247:Pregnancy / Q000097:blood*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction / Q000295:instrumentation; D014961:X-Ray Diffraction
chemical_list: D004967:Estrogens; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide