title: Chitosan coated alginate beads for the survival of microencapsulated Lactobacillus plantarum in pomegranate juice.
abstract: This work studied the effect of multi-layer coating of alginate beads on the survival of encapsulated Lactobacillus plantarum in simulated gastric solution and during storage in pomegranate juice at 4°C. Uncoated, single and double chitosan coated beads were examined. The survival of the cells in simulated gastric solution (pH 1.5) was improved in the case of the chitosan coated beads by 0.5-2 logs compared to the uncoated beads. The cell concentration in pomegranate juice after six weeks of storage was higher than 5.5logCFU/mL for single and double coated beads, whereas for free cells and uncoated beads the cells died after 4 weeks of storage. In simulated gastric solution, the size of the beads decreased and their hardness increased with time; however, the opposite trend was observed for pomegranate juice, indicating that there is no correlation between cell survival and the hardness of the beads.
authors.affiliation: Department of Food and Nutritional Sciences, The University of Reading, P.O. Box 226, Reading RG6 6AP, UK.
authors.forename: Sawaminee
authors.initials: S
authors.lastname: Nualkaekul
authors.forename: Dominique
authors.initials: D
authors.lastname: Lenton
authors.forename: Michael T
authors.initials: MT
authors.lastname: Cook
authors.forename: Vitaliy V
authors.initials: VV
authors.lastname: Khutoryanskiy
authors.forename: Dimitris
authors.initials: D
authors.lastname: Charalampopoulos
mesh_terms: D000464:Alginates / Q000737:chemistry*; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry*; D005750:Gastric Juice / Q000737:chemistry* / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000166:cytology / Q000378:metabolism*; D029561:Lythraceae / Q000737:chemistry*; D050296:Microbial Viability
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan