title: Chemokine receptor 7 via proline-rich tyrosine kinase-2 upregulates             the chemotaxis and migration ability of squamous cell carcinoma of the head and             neck.
abstract: Aberrant regulation in the chemotaxis and migration ability of cancer cells             is closely associated with their metastatic activity. The chemokine receptor 7             (CCR7) has recently been shown to play an important role in regional lymph node             metastasis of squamous cell carcinoma of the head and neck (SCCHN). In this study,             we examined the role of proline-rich tyrosine kinase-2 (Pyk2) in CCR7-induced             chemotaxis and migration ability of metastatic SCCHN cells. We showed that Pyk2             is overexpressed in squamous cell carcinoma of the head and neck. We also found             that CCR7 induced Pyk2 and cofilin activation. Inhibition of Pyk2 activity using             a pharmacological inhibitor, Tyrphostin A9, significantly attenuated CCR7-induced             Pyk2 tyrosine phosphorylation, activation of cofilin and sequentially abolished             F-actin rearrangment, diminished the chemotaxis and migration ability of SCCHN             cells. In summary, our data suggest that CCR7 via Pyk2 and cofilin regulates the             chemotaxis and migration ability of metastatic SCCHN cells.
authors.affiliation: Department of Oromaxillofacial-Head and Neck Surgery, School of Stomatology, China Medical University, Shenyang, Liaoning 110002, PR China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Yang
authors.forename: Fayu
authors.initials: F
authors.lastname: Liu
authors.forename: Zhongfei
authors.initials: Z
authors.lastname: Xu
authors.forename: Nan
authors.initials: N
authors.lastname: Guo
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Zheng
authors.forename: Changfu
authors.initials: C
authors.lastname: Sun
mesh_terms: D051339:Actin Depolymerizing Factors / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D002633:Chemotaxis; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology*; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D010766:Phosphorylation; D054400:Receptors, CCR7 / Q000378:metabolism*; D015398:Signal Transduction; D000077195:Squamous Cell Carcinoma of Head and Neck; D053634:TYK2 Kinase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D020032:Tyrphostins / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D051339:Actin Depolymerizing Factors; D000199:Actins; C515370:CCR7 protein, human; D054400:Receptors, CCR7; D020032:Tyrphostins; C406122:tyrphostin A9; D053634:TYK2 Kinase; C070139:TYK2 protein, human