title: Bone healing in critical-size defects treated with immediate transplant of fragmented autogenous white adipose tissue.
abstract: This study analyzed the influence of fragmented autogenous white subcutaneous adipose tissue (WSAT) on bone healing in critical-size defects created in rabbit calvaria. A 15-mm diameter defect was created in the calvaria of 42 rabbits, which were treated with 86 mm3 WSAT grafts or filled only with blood clots (control). Animals were euthanized at 7, 15, and 40 days postsurgery (n = 7), and the data were analyzed using histomorphometry and immunohistochemistry using the anti-CD34 and bone morphogenetic protein-2 (BMP2) antibodies. The calvaria treated with only blood clots demonstrated positivity to CD34 concentrated in endothelial cells, whereas the BMP2 were restricted to the borders of the defects. In contrast, the group treated with WSAT revealed fatty cells exhibiting positivity to BMP2 both in membrane and cytoplasm and intense quantities of stromal spindle cells that exhibited positivity to CD34 simultaneously to BMP2; these results together coincided with a larger bone matrix deposited in all postsurgery periods analyzed. These results revealed that the WSAT may be an alternative to craniofacial bone reconstruction because the adipose tissue exhibited prominent immunoexpression of BMP2, which was coexpressed with stromal CD34+ cells, indicating the plasticity of CD34+ stem cells into osteoblasts, and in the adipocytes, facts that suggest bone tissue development through cellular transdifferentiation from adipocytes.
authors.affiliation: Positivo University, Curitiba, Brazil.
authors.forename: Shaiene Patricia
authors.initials: SP
authors.lastname: Gomes
authors.forename: Tatiana Miranda
authors.initials: TM
authors.lastname: Deliberador
authors.forename: Carla Castiglia
authors.initials: CC
authors.lastname: Gonzaga
authors.forename: Luiz Gustavo
authors.initials: LG
authors.lastname: Klug
authors.forename: Lidiane
authors.initials: L
authors.lastname: da Costa Oliveira
authors.forename: Cícero
authors.initials: C
authors.lastname: de Andrade Urban
authors.forename: João Cesar
authors.initials: JC
authors.lastname: Zielak
authors.forename: Allan Fernando
authors.initials: AF
authors.lastname: Giovanini
mesh_terms: D000273:Adipose Tissue / Q000637:transplantation*; D000704:Analysis of Variance; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D016025:Bone Transplantation / Q000379:methods*; D002454:Cell Differentiation; D007124:Immunoenzyme Techniques; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D012886:Skull / Q000601:surgery*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D018952:Antigens, CD34; D055396:Bone Morphogenetic Protein 2