title: The structural role of the carrier protein--active controller or passive carrier.
abstract: Common to all FASs, PKSs and NRPSs is a remarkable component, the acyl or peptidyl carrier protein (A/PCP). These take the form of small individual proteins in type II systems or discrete folded domains in the multi-domain type I systems and are characterized by a fold consisting of three major α-helices and between 60-100 amino acids. This protein is central to these biosynthetic systems and it must bind and transport a wide variety of functionalized ligands as well as mediate numerous protein-protein interactions, all of which contribute to efficient enzyme turnover. This review covers the structural and biochemical characterization of carrier proteins, as well as assessing their interactions with different ligands, and other synthase components. Finally, their role as an emerging tool in biotechnology is discussed.
authors.affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK.
authors.forename: John
authors.initials: J
authors.lastname: Crosby
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Crump
mesh_terms: D000213:Acyl Carrier Protein / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D015394:Molecular Structure; D048630:Polyketide Synthases / Q000737:chemistry* / Q000378:metabolism*; D061065:Polyketides / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation
chemical_list: D000213:Acyl Carrier Protein; D061065:Polyketides; D048630:Polyketide Synthases