title: Comparison of Foxp3+ regulatory T cells and CD163+ macrophages in invasive and non-invasive extramammary Paget's disease.
abstract: Regulatory T cells (Tregs), identified by the expression of CD4, CD25 and Foxp3, together with immunosuppressive macrophages, such as CD163+ M2 macrophages, are involved in maintaining peripheral tolerance. The aim of this study was to elucidate the involvement of Tregs and CD163+ macrophages in invasive and non-invasive extramammary Paget's disease. The presence of CD4+CD25+Foxp3+ Tregs, CD163+ M2 macrophages and matrix metalloproteinase-9+ cells was examined immunohistologically in fixed sections of lesional skin from 10 patients with non-invasive extramammary Paget's disease and 7 patients with invasive extramammary Paget's disease. Fewer CD4+CD25+Foxp3+ Tregs were observed in non-invasive extramammary Paget's disease than in invasive extramammary Paget's disease. In contrast, higher numbers of CD163+ macrophages and metalloproteinase-9+ cells were detected only in invasive extramammary Paget's disease. These findings suggest that the induction of immunosuppressive cells in extramammary Paget's disease differs according to the tumour stage.
authors.affiliation: Department of Dermatology, Tohoku University Graduate School of Medicine, Sendai, Japan. tfujimura1@mac.com
authors.forename: Taku
authors.initials: T
authors.lastname: Fujimura
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kambayashi
authors.forename: Takanori
authors.initials: T
authors.lastname: Hidaka
authors.forename: Akira
authors.initials: A
authors.lastname: Hashimoto
authors.forename: Takahiro
authors.initials: T
authors.lastname: Haga
authors.forename: Setsuya
authors.initials: S
authors.lastname: Aiba
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D051858:Forkhead Transcription Factors / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis; D009361:Neoplasm Invasiveness; D010145:Paget Disease, Extramammary / Q000276:immunology* / Q000473:pathology; D011956:Receptors, Cell Surface / Q000032:analysis*; D012878:Skin Neoplasms / Q000276:immunology* / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D014408:Biomarkers, Tumor; D000098902:CD163 Antigen; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D011956:Receptors, Cell Surface; D020780:Matrix Metalloproteinase 9