title: [Mechanism of the apoptosis of rat pancreas islet β cell strain (INS-1 cells) induced by sodium arsenite].
abstract: OBJECTIVE
  To study mechanism of the apoptosis of rat pancreas islet β cell strain (INS-1 cells) induced by sodium arsenite.
  
  
  METHODS
  INS-1 cells were exposed to sodium arsenite at the different concentrations. MTT assay was used to detect the viability of INS-1 cells. The potentials on mitochondrial membrane and lysosome membrane of INS-1 cells were determined with the fluorescence spectrophotometer. The apoptotic levels of INS-1 cells exposed to sodium arsenite were observed by a fluorescence microscope and flow cytometry.
  
  
  RESULTS
  After exposure to sodium arsenite, the viability of INS-1 cells significantly decreased with the doses of sodium arsenite. At 24 h after exposure, the OD values of the mitochondrial membrane potentials declined observably with the doses of sodium arsenite (P < 0.01). At 48 h after exposure, the OD values of the lysosome membrane potentials significantly increased with the doses of sodium arsenite (P < 0.01). At 72 h after exposure, the apoptotic cells were observed under a fluorescence microscope and enhanced with the doses of sodium arsenite. The apoptosis cells with light blue, karyopyknosis, karyorrhexis, apoptotic body and chromatin concentration appeared. The results detected with flow cytometry indicated that after exposure, the apoptotic INS-1E cells significantly increased with the doses of sodium arsenite.
  
  
  CONCLUSIONS
  The sodium arsenite can induce the apoptosis of INS-1 cells through the mitochondria-lysosome pathway.
authors.affiliation: School of Public Health, Dalian Medical University, Dalian, Liaoning, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Pan
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Jiang
authors.forename: Lai-fu
authors.initials: LF
authors.lastname: Zhong
authors.forename: Cheng-yan
authors.initials: CY
authors.lastname: Geng
authors.forename: Xian-ce
authors.initials: XC
authors.lastname: Sun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D018053:Arsenites / Q000633:toxicity*; D002478:Cells, Cultured; D050417:Insulin-Secreting Cells / Q000187:drug effects*; D008247:Lysosomes / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism; D051381:Rats; D017670:Sodium Compounds / Q000633:toxicity*
chemical_list: D018053:Arsenites; D017670:Sodium Compounds; C017947:sodium arsenite