title: Immobilization of anti-aflatoxin B1 antibody by UV polymerization of aniline and aflatoxin B1 detection via electrochemical impedance spectroscopy.
abstract: In the study, we investigated the practicality of the UV polymerization of aniline for anti-aflatoxin B1 antibody immobilization, and utilization of the resulting biosensor in the impedimetric determination of aflatoxin B1. The anti-aflatoxin B 1 antibody was physically immobilized on gold electrodes by UV polymerization of aniline at a fixed wavelength. The biosensor was based on specific interaction anti-aflatoxin B1 - aflatoxin B1 recognition and investigation of this recognition event by electrochemical impedance spectroscopy. A calibration curve was obtained in a linear detection range 1-20 ng/mL aflatoxin B1. Finally, the biosensor was applied to analysis of a real food sample.
authors.affiliation: Ege University, Faculty of Science, Biochemistry Department, Bornova-İzmir, Turkey.
authors.forename: Erhan
authors.initials: E
authors.lastname: Dinçkaya
authors.forename: Özer
authors.initials: Ö
authors.lastname: Kinik
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Sezgintürk
authors.forename: Çağri
authors.initials: Ç
authors.lastname: Altuğ
authors.forename: Aylin
authors.initials: A
authors.lastname: Akkoca
mesh_terms: D016604:Aflatoxin B1 / Q000009:adverse effects / Q000032:analysis* / Q000276:immunology; D000814:Aniline Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D055910:Antibodies, Immobilized / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D002138:Calibration; D058266:Dielectric Spectroscopy / Q000379:methods; D005240:Feasibility Studies; D005504:Food Analysis; D005517:Foodborne Diseases / Q000209:etiology* / Q000517:prevention & control; D006801:Humans; D058105:Polymerization / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000814:Aniline Compounds; D055910:Antibodies, Immobilized; D000911:Antibodies, Monoclonal; D016604:Aflatoxin B1; C023650:aniline