title: Mice carrying a complete deletion of the talin2 coding sequence are viable and fertile.
abstract: Mice homozygous for several Tln2 gene targeted alleles are viable and fertile. Here we show that although the expression of talin2 protein is drastically reduced in muscle from these mice, other tissues continue to express talin2 albeit at reduced levels. We therefore generated a Tln2 allele lacking the entire coding sequence (Tln2(cd)). Tln2(cd/cd) mice were viable and fertile, and the genotypes of Tln2(cd/+) intercrosses were at the expected Mendelian ratio. Tln2(cd/cd) mice showed no major difference in body mass or the weight of the major organs compared to wild-type, although they displayed a mildly dystrophic phenotype. Moreover, Tln2(cd/cd) mouse embryo fibroblasts showed no obvious defects in cell adhesion, migration or proliferation. However, the number of Tln2(cd/cd) pups surviving to adulthood was variable suggesting that such mice have an underlying defect.
authors.affiliation: Department of Biochemistry, University of Leicester, Lancaster Road, Leicester LE1 9HN, UK.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Debrand
authors.forename: Francesco J
authors.initials: FJ
authors.lastname: Conti
authors.forename: Neil
authors.initials: N
authors.lastname: Bate
authors.forename: Lorraine
authors.initials: L
authors.lastname: Spence
authors.forename: Daniela
authors.initials: D
authors.lastname: Mazzeo
authors.forename: Catrin A
authors.initials: CA
authors.lastname: Pritchard
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Monkley
authors.forename: David R
authors.initials: DR
authors.lastname: Critchley
mesh_terms: D000818:Animals; D001835:Body Weight; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D005298:Fertility; D005347:Fibroblasts / Q000502:physiology; D017353:Gene Deletion; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009136:Muscular Dystrophies / Q000235:genetics / Q000473:pathology; D016608:Talin / Q000235:genetics / Q000502:physiology*
chemical_list: C518833:TLN2 protein, mouse; D016608:Talin