title: [Analysis of the genetic differences in the nucleoprotein between rabies virus and its vaccine strains in Guizhou province from year 2005 to 2010].
abstract: OBJECTIVE
  This study was to explore the differences in the nucleoprotein gene between rabies virus (RABV) and its vaccine strains in Guizhou province from year 2005 to 2010.
  
  
  METHODS
  Samples from 4 rabies patients and cerebral tissue samples of 28 rabies infected dogs were collected from different districts in Guizhou province between year 2005 and 2010. Direct Immunofluorescence Assay (DFA) and RT-nested PCR assay were applied to detect the overall length of N gene sequence. Meanwhile, based on the comparison between the homology and phylogenetic tree, the differences in N gene sequence between the prevalent RABV and the RABV vaccine strains collected from NCBI database in these years.
  
  
  RESULTS
  According to DFA and RT-nested PCR assay, the antigen and nucleic acid of the 21 dogs and 4 human samples were both confirmed positive; whose full length of N gene sequences were both 1353 bp. The homological analysis showed that the 25 strains of RABV virus and the RABV type I virus stored by GenBank database shared a high homology in N gene nucleotide and amino acid sequences, which were 89%-100% and 98%-100%, respectively. Besides, the homology between the 25 strains of RABV virus and its vaccines in nucleotide and amino acid sequences were separately 86%-95% and 96%-100%. The N gene of vaccines for livestock shared the highest homology with HEP-Flury strain in the nucleotide and amino acid, which were 88%-89% and 98%-99%, respectively. The vaccines for human use showed its greatest homology with the CTN strain in nucleotide (86%-100%) and amino acid (96%-100%). The phylogenetic tree analysis indicated that the 25 strains of RABV virus, RABV type I virus and the CTN vaccine strains constituted one individual cluster, which was least different from the CTN vaccine for human use.
  
  
  CONCLUSIONS
  The prevalent RABV virus, the vaccine HEP-Flury for livestock and the vaccine CTN for human use were found to be highly similar in N gene expression in Guizhou province from 2005 to 2010.
authors.affiliation: Guizhou Provincial Centers for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Shi-jun
authors.initials: SJ
authors.lastname: Li
authors.lastname: Yu-chun
authors.forename: Ding-ming
authors.initials: DM
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Tang
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Tao
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhuang
authors.forename: Jing-zhu
authors.initials: JZ
authors.lastname: Zhou
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Ke-cheng
authors.initials: KC
authors.lastname: Tian
authors.forename: Guang-peng
authors.initials: GP
authors.lastname: Tang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004285:Dogs; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D011818:Rabies / Q000662:veterinary / Q000821:virology*; D011819:Rabies Vaccines / Q000235:genetics*; D011820:Rabies virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D009698:Nucleoproteins; D012367:RNA, Viral; D011819:Rabies Vaccines