title: Screening for cholesterol-lowering probiotic based on deoxycholic acid removal pathway and studying its functional mechanisms in vitro.
abstract: Elevated serum cholesterol in humans is generally a risk factor correlated with the development of coronary heart disease (CHD). Reducing deoxycholic acid (DCA) content in the intestine can reduce serum cholesterol levels, which reduce the incidence of CHD. A total of 150 strains of lactic acid bacteria and bifidobacteria were isolated from human fecal samples. The DCA removal ability of these strains was evaluated. Results showed that 9 strains displayed above 10% DCA removal rate. The probiotic potentials of the 9 strains were evaluated. The strain Lactobacillus casei F0822 was screened out due to the stronger adhesion to HT-29 cells and tolerance to bile and acid. DCA removal for this strain resulted from that the S-layer protein locating the cell surface bound DCA. The FTIR spectra showed that the carboxyl group in DCA was the principal group by which DCA was bound to the S-layer protein of L. casei F0822. These findings suggested that L. casei F0822 is a better candidate probiotic strain, which has the potential to reduce human serum cholesterol levels.
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Guo
authors.forename: Lan-Wei
authors.initials: LW
authors.lastname: Zhang
authors.forename: Xue
authors.initials: X
authors.lastname: Han
authors.forename: Hua-Xi
authors.initials: HX
authors.lastname: Yi
authors.forename: Jing-Yan
authors.initials: JY
authors.lastname: Li
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Tuo
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Du
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Shan
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
mesh_terms: D000328:Adult; D001644:Bifidobacterium / Q000302:isolation & purification / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D003840:Deoxycholic Acid / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D006801:Humans; D007223:Infant; D056546:Lactobacillales / Q000302:isolation & purification / Q000378:metabolism*; D007780:Lacticaseibacillus casei; D008403:Mass Screening / Q000379:methods; D019936:Probiotics / Q000302:isolation & purification*
chemical_list: D003840:Deoxycholic Acid; D002784:Cholesterol