title: Cell adhesion molecule 1 (CADM1) is ubiquitously present in the endothelium and smooth muscle cells of the human macro- and micro-vasculature.
abstract: Cell adhesion molecule 1 (CADM1) is a member of the immunoglobulin cell adhesion molecule family. Recently, we identified CADM1 to be a novel risk factor for venous thrombosis in a large, protein C deficient, thrombophilic family and showed, for the first time, the expression of CADM1 in endothelial cells (Hasstedt et al. in Blood 114:3084-3091, 2009). To further investigate its role in venous thrombosis, as well as other vasculopathies, we undertook a systematic confocal microscopic investigation for the presence of CADM1 in the vasculature of 28 different human tissues. Paraffin embedded tissue sections were dual immunostained with an antibody against CADM1, together with an antibody against either von Willebrand factor (to identify endothelial cells), or α-smooth muscle actin (to identify smooth muscle cells). The results showed that CADM1 was ubiquitously present in endothelial cells and smooth muscle cells in the vasculature from all 28 tissues, though its representation in the various classes of vessels was tissue dependent.
authors.affiliation: Department of Pathology, University of Vermont College of Medicine, 89 Beaumont Avenue, Burlington, VT 05405, USA.
authors.forename: Kanayo
authors.initials: K
authors.lastname: Tatsumi
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Taatjes
authors.forename: Marilyn P
authors.initials: MP
authors.lastname: Wadsworth
authors.forename: Beth A
authors.initials: BA
authors.lastname: Bouchard
authors.forename: Edwin G
authors.initials: EG
authors.lastname: Bovill
mesh_terms: D000199:Actins / Q000032:analysis; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000378:metabolism*; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D055806:Microvessels / Q000166:cytology / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D014841:von Willebrand Factor / Q000032:analysis
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; C403514:CADM1 protein, human; D000075982:Cell Adhesion Molecule-1; D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; D014841:von Willebrand Factor