title: Isolation and characterization of a lipopeptide bioemulsifier produced by Pseudomonas nitroreducens TSB.MJ10 isolated from a mangrove ecosystem.
abstract: Pseudomonas nitroreducens TSB.MJ10 exhibiting growth and bioemulsifier production with 0.5% sodium benzoate as the sole carbon source was isolated from a mangrove ecosystem in the vicinity of a petroleum pump. The bioemulsifier is a lipopeptide that is stable over a pH range of 5-11 and a temperature range of 20-90°C and showed emulsifying activity in the presence of relatively high NaCl concentrations (up to 25%). The bioemulsifier formed stable emulsions with aliphatic (hexadecane, n-heptane, cyclohexane), aromatic (xylene, benzene, toluene) and petroleum (gasoline, diesel, kerosene, crude oil) compounds. It exhibited a maximum emulsification activity with weathered crude oil (97%) and was capable of transforming the rheological behavior of the pseudoplastic to a Newtonian fluid. The results reveal the potential of the bioemulsifier for use in bioremediation of hydrocarbons in marine environments and in enhanced oil recovery.
authors.affiliation: Department of Microbiology, Goa University, Taleigao Plateau, Goa 403 206, India.
authors.forename: Trelita
authors.initials: T
authors.lastname: de Sousa
authors.forename: Saroj
authors.initials: S
authors.lastname: Bhosle
mesh_terms: D032407:Avicennia / Q000382:microbiology*; D002244:Carbon / Q000494:pharmacology; D017753:Ecosystem; D045703:Emulsifying Agents / Q000302:isolation & purification*; D006838:Hydrocarbons / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D055666:Lipopeptides / Q000096:biosynthesis* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010578:Petroleum; D011549:Pseudomonas / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D012212:Rheology / Q000187:drug effects; D020160:Sodium Benzoate / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
chemical_list: D045703:Emulsifying Agents; D006838:Hydrocarbons; D055666:Lipopeptides; D010578:Petroleum; D012965:Sodium Chloride; D002244:Carbon; D020160:Sodium Benzoate