title: Mechanism of cadmium-induced cytotoxicity on the ZFL zebrafish liver cell line.
abstract: The cadmium ion (Cd²⁺) is a highly toxic metal ion; however, its hepatic toxic effects are not very well characterized in a systematic manner. In this study, a zebrafish liver cell line, ZFL was used as a model to investigate the mechanism of Cd²⁺-induced cytotoxicity on hepatocytes. The intracellular level of reactive oxygen species decreased following the administration of Cd²⁺; antioxidant levels and related enzyme activities and gene expression were detected, showing that the toxic effects of Cd²⁺ might not be coupled to oxidative stress. To understand the cytotoxic effects of Cd²⁺ on ZFL cells after Cd²⁺ exposure, a total of 77 differentially expressed proteins were detected by two-dimensional gel electrophoresis; 43 of them were further identified by MALDI-TOF-MS. The proteins that responded to Cd²⁺ in ZFL cells were related to stress response, transporters, regulation of transcription, redox homeostasis, or different signaling pathways, with half of these proteins having metal ion binding capabilities.
authors.affiliation: School of Life Sciences, Faculty of Science, Chinese University, Room 184, Science Centre South Block, Sha Tin, N.T., Hong Kong SAR, China.
authors.forename: Jin Yong
authors.initials: JY
authors.lastname: Zhu
authors.forename: King Ming
authors.initials: KM
authors.lastname: Chan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D005434:Flow Cytometry; D022781:Hepatocytes / Q000737:chemistry / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D042541:Intracellular Space / Q000737:chemistry / Q000187:drug effects; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000187:drug effects; D040901:Proteomics; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D015027:Zebrafish
chemical_list: D000975:Antioxidants; D020543:Proteome; D017382:Reactive Oxygen Species; D002104:Cadmium; D010088:Oxidoreductases