title: Basal and 3-methylcholanthrene-induced expression of cytochrome P450 1A, 1B and 1C genes in the Brazilian guppy, Poecilia vivipara.
abstract: In fish there are four cytochrome P450 (CYP1) subfamilies: CYP1A, CYP1B, CYP1C, and CYP1D. Here we cloned Poecilia vivipara CYP1A, with an inferred amino acid sequence 91% identical to CYP1A from the killifish Fundulus heteroclitus, another member of the Cypriniformes, and an important model in ecotoxicology. In addition, we examined the expression of CYP1A, CYP1B1, and CYP1C1 by qPCR in liver, gill, and intestine of adult P. vivipara injected with 3-methylcholanthrene (3-MC) or held in clean water (control group) for 24h. All three tissues examined showed basal expression of the three CYP1 genes. CYP1A was most strongly expressed in the liver, while CYP1B1, and CYP1C1 were most strongly expressed in the gill and intestine respectively. 3-MC induced CYP1A, CYP1B1, and CYP1C1 significantly (20-120-fold) in the three organs, consistent with the regulation of CYP1A, CYP1B1 and CYP1C1 via the aryl hydrocarbon receptor. Validation of CYP1 gene biomarkers in fish collected from a contaminated urban mangrove environment was confirmed with significant induction of CYP1A and CYP1C1 in gills (10-15-fold) and CYP1B1 in liver (23-fold), relative to fish from a control site. The responsiveness of these CYP1 genes indicates P. vivipara is suitable as a model for environmental toxicology studies and environmental assessment in Brazil.
authors.affiliation: Biochemistry Department, Universidade Federal de Santa Catarina, SC, Brazil. tarquindorrington@gmail.com
authors.forename: Tarquin
authors.initials: T
authors.lastname: Dorrington
authors.forename: Juliano
authors.initials: J
authors.lastname: Zanette
authors.forename: Flávia L
authors.initials: FL
authors.lastname: Zacchi
authors.forename: John J
authors.initials: JJ
authors.lastname: Stegeman
authors.forename: Afonso C D
authors.initials: AC
authors.lastname: Bainy
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001938:Brazil; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008748:Methylcholanthrene / Q000633:toxicity*; D011036:Poecilia / Q000235:genetics* / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D029941:Fish Proteins; D014874:Water Pollutants, Chemical; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D019363:Cytochrome P-450 CYP1A1