title: The identification of vitamin E homologues in medicinal plant samples using ESI(+)-LC-MS3.
abstract: The aim of this study was to elucidate the presence of vitamin E homologues in medicinal plants. To identify various homologues in the matrix of medicinal plant samples, a method for simultaneous determination was developed using ESI(+)-LC-MS3. A complete separation of each homologue was achieved within 20 min using a PFP column and an isocratic elution system of water/methanol (10:90, v/v) at a flow rate of 0.5 mL/min. The ESI-MS condition for each homologue was optimized, and the m/z value and the fragmentation pathway of each homologue were summarized. This LC-MS3 method made it possible to detect the homologues without the effect of matrix; therefore, high sensitive analysis was established, and then, the MS3 makes it possible to extract from plants with methanol only. The LC-MS3 method was applied to identify the eight vitamin E homologues in 11 medicinal plants.
authors.affiliation: Ochanomizu University , 2-1-1 Otsuka, Bunkyo-ku, Tokyo 112-8610, Japan. g1070520@edu.cc.ocha.ac.jp
authors.forename: Tomoko
authors.initials: T
authors.lastname: Inoue
authors.forename: Satoko
authors.initials: S
authors.lastname: Tatemori
authors.forename: Natsumi
authors.initials: N
authors.lastname: Muranaka
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Hirahara
authors.forename: Seiichi
authors.initials: S
authors.lastname: Homma
authors.forename: Takahisa
authors.initials: T
authors.lastname: Nakane
authors.forename: Akihito
authors.initials: A
authors.lastname: Takano
authors.forename: Yuri
authors.initials: Y
authors.lastname: Nomi
authors.forename: Yuzuru
authors.initials: Y
authors.lastname: Otsuka
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005052:Eucalyptus / Q000737:chemistry; D030001:Foeniculum / Q000737:chemistry; D020902:Hypericum / Q000737:chemistry; D031334:Melissa / Q000737:chemistry; D027541:Mentha / Q000737:chemistry; D018649:Ocimum basilicum / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D036625:Stevia / Q000737:chemistry; D024508:Tocotrienols / Q000032:analysis; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D024508:Tocotrienols; D014810:Vitamin E