title: miR-143 and miR-145 inhibit stem cell characteristics of PC-3 prostate             cancer cells.
abstract: Emerging evidence demonstrates that cancer stem cells (CSCs) are the critical drivers of tumor progression and metastasis. The microRNAs (miRNAs) may play a crucial role in repressing/promoting metastasis of cancer by regulating CSCs. A previous study showed that miR-143 and miR-145 play an important role in regulating bone metastasis of prostate cancer (PCa), but the exact mechanism of regulation of bone metastasis of PCa is not fully understood. In this study, we found that overexpression of miR-143 and miR-145 inhibited the cell viability and colony formation of PC-3 cells from PCa bone metastasis. Furthermore, miR-143 and miR-145 suppressed tumor sphere formation and expression of CSC markers and 'stemness' factors including CD133, CD44, Oct4, c-Myc and Klf4 in PC-3 cells. The study further found that miR-143 and miR-145 inhibit bone invasion and tumorigenicity of PC-3 cells in vivo. Collectively, these findings demonstrate that miR-143 and miR-145 inhibit CSC properties of PC-3 cells and suggest that miR-143 and miR-145 may play a significant role in the bone metastasis progression of PCa by regulating CSC characteristics.
authors.affiliation: Department of Orthopaedic Surgery/Orthopaedic Research Institute, The First Affiliated Hospital of Sun Yat-sen University, 510080 Guangzhou, Guangdong Province, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.forename: Yubo
authors.initials: Y
authors.lastname: Tang
authors.forename: Dong
authors.initials: D
authors.lastname: Ren
authors.forename: Xuenong
authors.initials: X
authors.lastname: Zou
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Peng
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000096:biosynthesis; D001859:Bone Neoplasms / Q000556:secondary; D045744:Cell Line, Tumor; D002470:Cell Survival; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D006023:Glycoproteins / Q000096:biosynthesis; D057809:HEK293 Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000096:biosynthesis; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000502:physiology*; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis; D010455:Peptides; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D018874:Spheroids, Cellular
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D018960:Hyaluronan Receptors; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; C542359:MIRN143 microRNA, human; C541010:MIRN145 microRNA, human; C489427:MYC protein, human; D035683:MicroRNAs; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C000606023:PROM1 protein, human; D010455:Peptides; D016271:Proto-Oncogene Proteins c-myc