title: Targeting collagen strands by photo-triggered triple-helix hybridization.
abstract: Collagen remodeling is an integral part of tissue development, maintenance, and regeneration, but excessive remodeling is associated with various pathologic conditions. The ability to target collagens undergoing remodeling could lead to new diagnostics and therapeutics as well as applications in regenerative medicine; however, such collagens are often degraded and denatured, making them difficult to target with conventional approaches. Here, we present caged collagen mimetic peptides (CMPs) that can be photo-triggered to fold into triple helix and bind to collagens denatured by heat or by matrix metalloproteinase (MMP) digestion. Peptide-binding assays indicate that the binding is primarily driven by stereo-selective triple-helical hybridization between monomeric CMPs of high triple-helical propensity and denatured collagen strands. Photo-triggered hybridization allows specific staining of collagen chains in protein gels as well as photo-patterning of collagen and gelatin substrates. In vivo experiments demonstrate that systemically delivered CMPs can bind to collagens in bones, as well as prominently in articular cartilages and tumors characterized by high MMP activity. We further show that CMP-based probes can detect abnormal bone growth activity in a mouse model of Marfan syndrome. This is an entirely new way to target the microenvironment of abnormal tissues and could lead to new opportunities for management of numerous pathologic conditions associated with collagen remodeling and high MMP activity.
authors.affiliation: Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Foss
authors.forename: Daniel D
authors.initials: DD
authors.lastname: Summerfield
authors.forename: Jefferson J
authors.initials: JJ
authors.lastname: Doyle
authors.forename: Collin M
authors.initials: CM
authors.lastname: Torok
authors.forename: Harry C
authors.initials: HC
authors.lastname: Dietz
authors.forename: Martin G
authors.initials: MG
authors.lastname: Pomper
authors.forename: S Michael
authors.initials: SM
authors.lastname: Yu
mesh_terms: D000818:Animals; D032701:Biomimetics; D001842:Bone and Bones / Q000473:pathology*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D003094:Collagen / Q000378:metabolism / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D008382:Marfan Syndrome / Q000175:diagnosis* / Q000503:physiopathology; D020782:Matrix Metalloproteinases / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D008958:Models, Molecular; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D010777:Photochemistry; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D003094:Collagen; D020782:Matrix Metalloproteinases