title: Vasopressin-induced intracellular Ca²⁺ concentration responses in non-neuronal cells of the rat dorsal root ganglion.
abstract: Arginine-vasopressin (AVP) is a nonapeptide of hypothalamic origin that has been shown to exert many important cognitive and physiological functions in neurons and terminals of both the central and peripheral nervous system (CNS and PNS). Here we report for the first time that AVP induced an increase in intracellular Ca²⁺ concentration ([Ca²⁺](i)) in non-neuronal cells isolated from the rat dorsal root ganglion (DRG) and cultured in vitro. The ratiometric [Ca²⁺](i) measurements showed that AVP evoked [Ca²⁺](i) responses in the non-neuronal cells and these concentration-dependent (100 pM to 1 μM) responses increased with days in vitro in culture, reaching a maximum amplitude after 4-5 day. Immunostaining by anti-S-100 antibody revealed that more than 70% of S-100 positive cells were AVP-responsive, indicating that glial cells responded to AVP and increased their [Ca²⁺](i). The responses were inhibited by depletion of the intracellular Ca²⁺ stores or in the presence of inhibitors of phospholipase C, indicating a metabotropic response involving inositol trisphosphate, and were mediated by the V₁ subclass of AVP receptors, as evidenced by the use of the specific blockers for V₁ and OT receptors, (d(CH₂)₅¹,Tyr(Me)²,Arg⁸)-Vasopressin and (d(CH₂)₅¹,Tyr(Me)²,Thr⁴,Orn⁸,des-Gly-NH₂⁹)-Vasotocin, respectively. V(1a) but not V(1b) receptor mRNA was expressed sustainably through the culture period in cultured DRG cells. These results suggest that AVP modulates the activity of DRG glial cells via activation of V(1a) receptor.
authors.affiliation: Department of Veterinary Physiology, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Moriya
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Kayano
authors.forename: Naoki
authors.initials: N
authors.lastname: Kitamura
authors.forename: Yoshinao Z
authors.initials: YZ
authors.lastname: Hosaka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Asano
authors.forename: Oksana
authors.initials: O
authors.lastname: Forostyak
authors.forename: Alexei
authors.initials: A
authors.lastname: Verkhratsky
authors.forename: Cedric
authors.initials: C
authors.lastname: Viero
authors.forename: Govindan
authors.initials: G
authors.lastname: Dayanithi
authors.forename: Emil C
authors.initials: EC
authors.lastname: Toescu
authors.forename: Izumi
authors.initials: I
authors.lastname: Shibuya
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000166:cytology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009457:Neuroglia / Q000187:drug effects*; D011189:Potassium Chloride / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017483:Receptors, Vasopressin / Q000235:genetics / Q000378:metabolism; D009418:S100 Proteins / Q000378:metabolism; D013997:Time Factors; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D014667:Vasopressins / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D017483:Receptors, Vasopressin; D009418:S100 Proteins; D014662:Vasoconstrictor Agents; C069944:vasopressin, des-Gly-; D014667:Vasopressins; D001127:Arginine Vasopressin; D011189:Potassium Chloride; C068622:argipressin,(1-mercaptocyclohexaneacetic acid)(1)-O-ethyl-Tyr(2)-; D002118:Calcium