title: 19F-NMR, 1H-NMR, and fluorescence studies of interaction between 5-fluorouracil and polyglycerol dendrimers.
abstract: Polyglycerol dendrimers (PGDs), which exhibit a well-defined structure consisting of only glycerol units, were examined as a host molecule of 5-fluorouracil (5-Fu) used as a model anticancer drug. (19)F- and (1)H-NMR titrations and fluorescence measurements were performed to estimate the molecular interaction between PGDs and 5-Fu in a buffer. Results of the NMR titrations revealed that PGD of generation 3 (PGD-G3) encapsulated 5-Fu in the buffer, whereas PGD-G2 and G1 partially incorporated 5-Fu molecule into the space. Fluorescent spectra of 5-Fu in the presence of PGD-G3 indicated that the diketo (lactam) form of 5-Fu changed to the enol-keto (lactim) form of 5-Fu, suggesting attraction of the imine proton of 5-Fu by ether oxygen of PGD-G3. Therefore, the encapsulation state of 5-Fu in PGDs at molecular level was modulated by the well-defined branched structure depending on the generation of PGDs.
authors.affiliation: Graduate School of Engineering, Kobe University, 1-1, Rokkoudai-cho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Haejoo
authors.initials: H
authors.lastname: Lee
authors.forename: Tooru
authors.initials: T
authors.lastname: Ooya
mesh_terms: D050091:Dendrimers / Q000737:chemistry*; D005453:Fluorescence; D005461:Fluorine / Q000737:chemistry; D005472:Fluorouracil / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry*; D011522:Protons
chemical_list: D050091:Dendrimers; D011108:Polymers; D011522:Protons; C043941:polyglycerol; D005461:Fluorine; D005990:Glycerol; D005472:Fluorouracil