title: The combined effect of acetylation and glycation on the chaperone and anti-apoptotic functions of human α-crystallin.
abstract: N(ε)-acetylation occurs on select lysine residues in α-crystallin of the human lens and alters its chaperone function. In this study, we investigated the effect of N(ε)-acetylation on advanced glycation end product (AGE) formation and consequences of the combined N(ε)-acetylation and AGE formation on the function of α-crystallin. Immunoprecipitation experiments revealed that N(ε)-acetylation of lysine residues and AGE formation co-occurs in both αA- and αB-crystallin of the human lens. Prior acetylation of αA- and αB-crystallin with acetic anhydride (Ac(2)O) before glycation with methylglyoxal (MGO) resulted in significant inhibition of the synthesis of two AGEs, hydroimidazolone (HI) and argpyrimidine. Similarly, synthesis of ascorbate-derived AGEs, pentosidine and N(ε)-carboxymethyl lysine (CML), was inhibited in both proteins by prior acetylation. In all cases, inhibition of AGE synthesis was positively related to the degree of acetylation. While prior acetylation further increased the chaperone activity of MGO-glycated αA-crystallin, it inhibited the loss of chaperone activity by ascorbate-glycation in both proteins. BioPORTER-mediated transfer of αA- and αB-crystallin into CHO cells resulted in significant protection against hyperthermia-induced apoptosis. This effect was enhanced in acetylated and MGO-modified αA- and αB-crystallin. Caspase-3 activity was reduced in α-crystallin transferred cells. Glycation of acetylated proteins with either MGO or ascorbate produced no significant change in the anti-apoptotic function. Collectively, these data demonstrate that lysine acetylation and AGE formation can occur concurrently in α-crystallin of human lens, and that lysine acetylation improves anti-apoptotic function of α-crystallin and prevents ascorbate-mediated loss of chaperone function.
authors.affiliation: Department of Ophthalmology and Visual Sciences, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
authors.forename: Rooban B
authors.initials: RB
authors.lastname: Nahomi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Oya-Ito
authors.forename: Ram H
authors.initials: RH
authors.lastname: Nagaraj
mesh_terms: D000107:Acetylation; D020816:Amino Acid Motifs; D017209:Apoptosis; D006031:Glycosylation; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology* / Q000378:metabolism*; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D038202:alpha-Crystallin A Chain / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D038203:alpha-Crystallin B Chain / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D018832:Molecular Chaperones; D038202:alpha-Crystallin A Chain; D038203:alpha-Crystallin B Chain