title: Resveratrol inhibits proliferation of primary rat hepatocytes in G0/G1 by inhibiting DNA synthesis.
abstract: Resveratrol is a phytoalexin that has been           shown to inhibit cell proliferation of several cancer           cell lines. In some cases this inhibition was specific           for the transformed cells when compared with normal           cells of the same tissue. To test whether this was           the case in rat hepatocytes, we exposed primary rat           hepatocytes in culture and transformed rat hepatic           cells to this compound and studied its effect on cell           proliferation, measuring deoxy-bromouridine incorporation           and total DNA. We also studied the effect of           resveratrol on the cell cycle of normal and transformed           rat hepatocytes. We observed that resveratrol           inhibited proliferation in a dose-dependent manner           in both cases, with no differential action in the           transformed cells compared to the normal ones. This           compound arrested the cell cycle in G0/G1 in primary           hepatocytes, while it arrested the cell cycle in G2/M           in transformed cells. Transformed hepatocytes showed           accumulation of cells in the S phase of the cell cycle.
authors.affiliation: Departamento de Fisiología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, Spain. ja.rubiolo@usc.es
authors.forename: J A
authors.initials: JA
authors.lastname: Rubiolo
authors.forename: H
authors.initials: H
authors.lastname: López-Alonso
authors.forename: V
authors.initials: V
authors.lastname: Martín-Vázquez
authors.forename: N M
authors.initials: NM
authors.lastname: Fol-Rodríguéz
authors.forename: M R
authors.initials: MR
authors.lastname: Vieytes
authors.forename: L M
authors.initials: LM
authors.lastname: Botana
authors.forename: F V
authors.initials: FV
authors.lastname: Vega
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D016193:G1 Phase / Q000187:drug effects*; D016195:G2 Phase / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D051381:Rats; D016192:Resting Phase, Cell Cycle / Q000187:drug effects*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D004247:DNA; D000077185:Resveratrol