title: Preparation of synthetically challenging nucleotides using cyanoethyl P-imidazolides and microwaves.
abstract: We describe a general method for the elongation of nucleoside oligophosphate chains by means of cyanoethyl (CE) phosphorimidazolides. Though the method requires a phosphorylation and subsequent deprotection reaction, both steps could be achieved in one pot without isolation/purification of the initial phosphorylation product. We have also found that pyrophosphate bond formation by this method is significantly accelerated by microwave irradiation.
authors.affiliation: Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland.
authors.forename: Malwina
authors.initials: M
authors.lastname: Strenkowska
authors.forename: Przemyslaw
authors.initials: P
authors.lastname: Wanat
authors.forename: Marcin
authors.initials: M
authors.lastname: Ziemniak
authors.forename: Jacek
authors.initials: J
authors.lastname: Jemielity
authors.forename: Joanna
authors.initials: J
authors.lastname: Kowalska
mesh_terms: D007093:Imidazoles / Q000737:chemistry*; D007202:Indicators and Reagents; D008872:Microwaves; D015394:Molecular Structure; D009570:Nitriles / Q000737:chemistry*; D009711:Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D007093:Imidazoles; D007202:Indicators and Reagents; D009570:Nitriles; D009711:Nucleotides