title: Development of quantitative receptor-ligand binding assay for use as a tool to estimate immune responses against Plasmodium vivax Duffy binding protein Region II.
abstract: Antibodies generated against Region II of Plasmodium vivax Duffy binding protein (PvRII) can block binding of this parasite ligand to its receptor, the Duffy antigen receptor for chemokines (DARC), and prevent erythrocyte infection by the parasite. An in vitro functional assay that can serve as an immune correlate of an antigen activity is an important tool to guide vaccine development. We describe here the development of a quantitative binding assay and its use to study immune responses against PvRII. The assay was used to test anti-PvRII mouse sera, and was found a useful tool for quantitative estimation of anti-PvRII blocking antibodies.
authors.affiliation: Malaria Group, International Centre for Genetic Engineering & Biotechnology, New Delhi, India.
authors.forename: Ahmad Rushdi
authors.initials: AR
authors.lastname: Shakri
authors.forename: M Moshahid A
authors.initials: MM
authors.lastname: Rizvi
authors.forename: Chetan E
authors.initials: CE
authors.lastname: Chitnis
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000032:analysis / Q000276:immunology; D000913:Antibodies, Protozoan / Q000032:analysis* / Q000276:immunology*; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D001665:Binding Sites; D002478:Cells, Cultured; D057809:HEK293 Cells; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008024:Ligands; D051379:Mice; D008807:Mice, Inbred BALB C; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification
chemical_list: D019138:Antibodies, Blocking; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C066001:Duffy antigen binding protein, Plasmodium; D007106:Immune Sera; D008024:Ligands; D015800:Protozoan Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins