title: Evaluation of the Tetracore Orthopox BioThreat® antigen detection assay using laboratory grown orthopoxviruses and rash illness clinical specimens.
abstract: The commercially available Orthopox BioThreat® Alert assay for orthopoxvirus (OPV) detection is piloted. This antibody-based lateral-flow assay labels and captures OPV viral agents to detect their presence. Serial dilutions of cultured Vaccinia virus (VACV) and Monkeypox virus (MPXV) were used to evaluate the sensitivity of the Tetracore assay by visual and quantitative determinations; specificity was assessed using a small but diverse set of diagnostically relevant blinded samples from viral lesions submitted for routine OPV diagnostic testing. The BioThreat® Alert assay reproducibly detected samples at concentrations of 10(7)pfu/ml for VACV and MPXV and positively identified samples containing 10(6)pfu/ml in 4 of 7 independent experiments. The assay correctly identified 9 of 11 OPV clinical samples and had only one false positive when testing 11 non-OPV samples. Results suggest applicability for use of the BioThreat® Alert assay as a rapid screening assay and point of care diagnosis for suspect human monkeypox cases.
authors.affiliation: Centers for Disease Control and Prevention, Division of High-Consequence Pathogens and Pathology, Poxvirus and Rabies Branch, 1600 Clifton Road NE, Atlanta, GA 30333, United States. gbu3@cdc.gov
authors.forename: Michael B
authors.initials: MB
authors.lastname: Townsend
authors.forename: Adam
authors.initials: A
authors.lastname: MacNeil
authors.forename: Mary G
authors.initials: MG
authors.lastname: Reynolds
authors.forename: Christine M
authors.initials: CM
authors.lastname: Hughes
authors.forename: Victoria A
authors.initials: VA
authors.lastname: Olson
authors.forename: Inger K
authors.initials: IK
authors.lastname: Damon
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Karem
mesh_terms: D001681:Biological Assay; D023482:Bioterrorism; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D045908:Mpox, Monkeypox / Q000175:diagnosis / Q000821:virology; D008993:Monkeypox virus / Q000235:genetics; D018155:Orthopoxvirus / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011213:Poxviridae Infections / Q000175:diagnosis* / Q000821:virology; D014616:Vaccinia virus / Q000235:genetics
chemical_list: D004279:DNA, Viral