title: The spontaneously hypertensive and Wistar Kyoto rat models of ADHD exhibit sub-regional differences in dopamine release and uptake in the striatum and nucleus accumbens.
abstract: The most widely used animal model of attention-deficit/hyperactivity disorder (ADHD) is the spontaneously hypertensive rat (SHR/NCrl), which best represents the combined subtype (ADHD-C). Recent evidence has revealed that a progenitor strain, the Wistar Kyoto from Charles River Laboratories (WKY/NCrl), is useful as a model of the inattentive subtype (ADHD-PI) and the Wistar Kyoto from Harlan Laboratories (WKY/NHsd) and the Sprague Dawley (SD) have been suggested as controls. Dopamine (DA) dysfunction in the striatum (Str) and nucleus accumbens core (NAc) is thought to play a significant role in the pathophysiology of ADHD but data obtained with the SHR is equivocal. Using high-speed chronoamperometric recordings with carbon fiber microelectrodes, we found that the SHR/NCrl displayed decreased KCl-evoked DA release versus the WKY/NCrl model of ADHD-PI in the dorsal Str. The WKY/NCrl and the WKY/NHsd control did not differ from each other; however, the control SD released less DA than the WKY/NCrl model of ADHD-PI in the dorsal Str and less than the control WKY/NHsd in the intermediate Str. The SHR/NCrl had faster DA uptake in the ventral Str and NAc versus both control strains, while the WKY/NCrl model of ADHD-PI exhibited faster DA uptake in the NAc versus the SD control. These results suggest that increased surface expression of DA transporters may explain the more rapid uptake of DA in the Str and NAc of these rodent models of ADHD.
authors.affiliation: Department of Anatomy & Neurobiology, University of Kentucky College of Medicine, 138 Leader Avenue, Lexington, KY 40506, USA.
authors.forename: Erin M
authors.initials: EM
authors.lastname: Miller
authors.forename: Francois
authors.initials: F
authors.lastname: Pomerleau
authors.forename: Peter
authors.initials: P
authors.lastname: Huettl
authors.forename: Vivienne A
authors.initials: VA
authors.lastname: Russell
authors.forename: Greg A
authors.initials: GA
authors.lastname: Gerhardt
authors.forename: Paul E A
authors.initials: PE
authors.lastname: Glaser
mesh_terms: D000818:Animals; D001289:Attention Deficit Disorder with Hyperactivity / Q000378:metabolism* / Q000523:psychology*; D003627:Data Interpretation, Statistical; D004298:Dopamine / Q000378:metabolism* / Q000502:physiology; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008839:Microelectrodes; D008845:Microinjections; D017072:Neostriatum / Q000187:drug effects / Q000378:metabolism*; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D015398:Signal Transduction
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D011189:Potassium Chloride; D004298:Dopamine