title: Early stages of trachea healing process: (immuno/lectin) histochemical monitoring of selected markers and adhesion/growth-regulatory endogenous lectins.
abstract: Tracheotomy may be associated with numerous acute and chronic complications including extensive formation of granulation tissue. The emerging functional versatility of the adhesion/growth-regulatory galectins prompted us to perform a histochemical study of wound healing using rat trachea as model. By using non-cross-reactive antibodies and the labelled tissue lectins we addressed the issue of the presence and regulation of galectin reactivity during trachea wound healing. Beside localization of high-molecular-weight keratin, wide-spectrum cytokeratin, keratins 10 and 14, α-smooth muscle actin, vimentin, fibronectin, and Sox-2, galectins -1, -2, and -3 and their reactivity profiles were measured in frozen sections of wounded and control trachea specimens 7, 14, and 28 days after trauma. A clear trend for decreased galectin-1 presence and increased reactivity for galectin-1 was revealed from day 7 to day 28. Sox-2-positive cells were present after seven days and found in the wound bed. Interestingly, several similarities were observed in comparison to skin wound healing including regulation of galectin-1 parameters.
authors.affiliation: 1st Department of Anesthesiology and Intensive Medicine, Pavol Jozef Šafárik University and Louis Pasteur University Hospital, Košice, Slovak Republic.
authors.forename: T
authors.initials: T
authors.lastname: Grendel
authors.forename: J
authors.initials: J
authors.lastname: Sokolský
authors.forename: A
authors.initials: A
authors.lastname: Vaščáková
authors.forename: V
authors.initials: V
authors.lastname: Hudák
authors.forename: M
authors.initials: M
authors.lastname: Chovanec
authors.forename: F
authors.initials: F
authors.lastname: Sabol
authors.forename: S
authors.initials: S
authors.lastname: André
authors.forename: H
authors.initials: H
authors.lastname: Kaltner
authors.forename: H-J
authors.initials: HJ
authors.lastname: Gabius
authors.forename: M
authors.initials: M
authors.lastname: Frankovičová
authors.forename: P
authors.initials: P
authors.lastname: Lenčeš
authors.forename: J
authors.initials: J
authors.lastname: Betka
authors.forename: K
authors.initials: K
authors.lastname: Smetana
authors.forename: P
authors.initials: P
authors.lastname: Gál
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002448:Cell Adhesion; D004195:Disease Models, Animal; D037161:Galectins / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000473:pathology; D014132:Trachea / Q000473:pathology / Q000502:physiology*; D014139:Tracheostomy / Q000009:adverse effects; D014140:Tracheotomy / Q000009:adverse effects; D014945:Wound Healing / Q000502:physiology*
chemical_list: D015415:Biomarkers; D037161:Galectins; D037102:Lectins