title: Development and validation of a capillary electrophoresis method with capacitively coupled contactless conductivity detection (CE-C(4) D) for the analysis of amikacin and its related substances.
abstract: Amikacin is a semisynthetic aminoglycoside antibiotic derived from kanamycin A that lacks a strong UV absorbing chromophore or fluorophore. Due to the physicochemical properties of amikacin and its related substances, CE in combination with capacitively coupled contactless conductivity detection (CE-C(4) D) was chosen. The optimized separation method uses a BGE composed of 20 mM MES adjusted to pH 6.6 by l-histidine and 0.3 mM CTAB that was added as flow modifier in a concentration below the CMC. Ammonium acetate 20 mg.L(-1) was used as internal standard. 30 kV was applied in reverse polarity on a fused silica capillary (73/48 cm; 75 μm id). The optimized separation was obtained in less than 6 min with good linearity (R(2) = 0.9996) for amikacin base. It shows a good precision expressed as RSD on relative peak areas equal to 0.1 and 0.7% for intraday and interday, respectively. The LOD and LOQ are 0.5 mg.L(-1) and 1.7 mg.L(-1) , respectively.
authors.affiliation: Laboratory for Pharmaceutical Analysis, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Leuven, Belgium.
authors.forename: Mohamed Nouri
authors.initials: MN
authors.lastname: El-Attug
authors.forename: Erwin
authors.initials: E
authors.lastname: Adams
authors.forename: Ann
authors.initials: A
authors.lastname: Van Schepdael
mesh_terms: D000583:Amikacin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D009994:Osmolar Concentration; D012043:Regression, Psychology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D000583:Amikacin