title: Sevoflurane exposure in 7-day-old rats affects neurogenesis, neurodegeneration and neurocognitive function.
abstract: OBJECTIVE
  Sevoflurane is widely used in pediatric anesthesia and former studies showed that it causes neurodegeneration in the developing brain. The present study was carried out to investigate the effects of sevoflurane on neurogenesis, neurodegeneration and behavior.
  
  
  METHODS
  We administered 5-bromodeoxyuridine, an S-phase marker, before, during, and after 4 h of sevoflurane given to rats on postnatal day 7 to assess dentate gyrus progenitor proliferation and Fluoro-Jade staining for degeneration. Spatial reference memory was tested 2 and 6 weeks after anesthesia.
  
  
  RESULTS
  Sevoflurane decreased progenitor proliferation and increased cell death until at least 4 days after anesthesia. Spatial reference memory was not affected at 2 weeks but was affected at 6 weeks after sevoflurane administration.
  
  
  CONCLUSIONS
  Sevoflurane reduces neurogenesis and increases the death of progenitor cells in developing brain. This might mediate the late-onset neurocognitive outcome after sevoflurane application.
authors.affiliation: Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.forename: Zhanggang
authors.initials: Z
authors.lastname: Xue
authors.forename: Jing
authors.initials: J
authors.lastname: Cang
mesh_terms: D018685:Anesthetics, Inhalation / Q000008:administration & dosage* / Q000633:toxicity; D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation / Q000187:drug effects; D003071:Cognition / Q000187:drug effects* / Q000502:physiology; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D008738:Methyl Ethers / Q000008:administration & dosage* / Q000633:toxicity; D009410:Nerve Degeneration / Q000139:chemically induced* / Q000473:pathology; D055495:Neurogenesis / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077149:Sevoflurane; D013234:Stem Cells / Q000187:drug effects / Q000473:pathology
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane