title: Application of embryonic and adult zebrafish for nanotoxicity assessment.
abstract: As an emerging model for toxicological studies, zebrafish has been explored for nanotoxicity assessment. In addition to endpoint examination of embryo/fish mortality and/or developmental disorders, molecular analyses of differential gene expression have also been employed to evaluate toxic effects associated with the exposure to nanomaterials. Here, we describe zebrafish-based assays, including both embryo and adult, for evaluation of nanotoxicity caused by metal oxide nanoparticles (NPs), in particular, zinc oxide (ZnO) and titanium oxide (TiO(2)) nanoparticles.
authors.affiliation: Center for Infectious Disease and Vaccinology, The Biodesign Institute, Arizona State University, Tempe, AZ, USA.
authors.forename: Jiangxin
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Zhu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Chen
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D004625:Embryo, Nonmammalian / Q000187:drug effects*; D004781:Environmental Exposure; D007477:Ions; D008670:Metals / Q000032:analysis / Q000633:toxicity; D053758:Nanoparticles / Q000633:toxicity*; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000166:cytology / Q000187:drug effects; D010316:Particle Size; D012313:RNA / Q000302:isolation & purification; D017382:Reactive Oxygen Species / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012996:Solutions; D013535:Suspensions; D014018:Tissue Distribution / Q000187:drug effects; D018675:Toxicity Tests / Q000379:methods*; D015027:Zebrafish / Q000196:embryology*
chemical_list: D007477:Ions; D008670:Metals; D017382:Reactive Oxygen Species; D012996:Solutions; D013535:Suspensions; D012313:RNA