title: New carbosilane polymers with interacting ferrocenes as support and bioelectrocatalysts of oxidases to develop versatile and specific amperometric biodevices.
abstract: In this work, the bioelectrocatalytical properties and kinetic characteristics of new oxidase amperometric biosensors based on two different ferrocene functionalized carbosilane polymers, polydiallylmethylsilane (PDAMS) and polymethyldiundecenylsilane (PMDUS) are described. In the development of these biodevices, glucose oxidase has been used as example of oxidase enzyme, and two different immobilization procedures have been studied. The polymer-modified electrodes act as efficient transducers for glucose sensing in anodic and cathodic aerobic conditions and also in anodic anaerobic conditions, and this fact turns them into useful devices for a wide field of applications. PMDUS has shown to be the bioelectrocatalyst with best kinetic and analytical properties in aerobic media while PDAMS was better in anaerobic conditions. The best aerobic biosensor developed displayed a strictly linear range from 0 to 3.0 mM, a detection limit of 7.8 μM and a response time less than 2 s in an ascorbate interference free work potential interval. The apparent Michaelis-Menten constant was calculated to be 1.36 mM according to the Lineweaver-Burk equation.
authors.affiliation: Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, Madrid, Spain. pilar.garcia.armada@upm.es
authors.forename: M Pilar García
authors.initials: MP
authors.lastname: Armada
authors.forename: Almudena
authors.initials: A
authors.lastname: Jiménez
authors.forename: José
authors.initials: J
authors.lastname: Losada
authors.forename: Beatriz
authors.initials: B
authors.lastname: Alonso
authors.forename: Carmen M
authors.initials: CM
authors.lastname: Casado
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000295:instrumentation*; D002417:Cattle; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005296:Ferrous Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D007700:Kinetics; D000075163:Metallocenes; D008958:Models, Molecular; D010984:Platinum / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011487:Protein Conformation; D015203:Reproducibility of Results; D012821:Silanes / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D000075163:Metallocenes; D011108:Polymers; D012821:Silanes; C504072:carbosilane; D010984:Platinum; D005949:Glucose Oxidase; D005947:Glucose; C004998:ferrocene