title: Real-time PCR and pyrosequencing for differentiation of medically relevant Bartonella species.
abstract: Multiple Bartonella species cause disease in humans. Although fast and accurate species differentiation could inform effective treatment interventions, species-level diagnosis of Bartonella infections is not typical. Here we describe a real-time PCR and pyrosequencing based algorithm for rapid differentiation of at least 11 medically relevant Bartonella spp.
authors.affiliation: Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.
authors.forename: Sarah N
authors.initials: SN
authors.lastname: Buss
authors.forename: Linda L
authors.initials: LL
authors.lastname: Gebhardt
authors.forename: Kimberlee A
authors.initials: KA
authors.lastname: Musser
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D001473:Bartonella / Q000145:classification* / Q000235:genetics*; D001474:Bartonella Infections / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods*