title: Phospholipid fingerprints of milk from different mammalians determined by 31P NMR: towards specific interest in human health.
abstract: Our objective was to identify and quantify phospholipids in milk from different species (human HM, cow CoM, camel CaM, and mare MM) using an optimised (31)P NMR spectroscopy procedure. The phospholipid fingerprints were species-specific with a broader variety of classes found in HM and MM; HM and CaM were richer in sphingomyelin (78.3 and 117.5μg/ml) and plasmalogens (27.3 and 24μg/ml), possibly important for infant development. Total phospholipid content was higher in CaM (0.503mM) and lower in MM (0.101mM) compared to HM (0.324mM) or CoM (0.265mM). Our optimised method showed good sensitivity, high resolution, and easy sample preparation with minimal loss of target molecules. It is suitable for determining the accurate composition of a large number of bioactive phospholipids with putative health benefits, including plasmalogens, and should aid in selecting appropriate ingredient sources for infant milk substitutes or fortifiers, and for functional foods dedicated to adults.
authors.affiliation: INSERM, U476, F-13385 Marseille, France. cyrielle.garcia@univ-amu.fr
authors.forename: Cyrielle
authors.initials: C
authors.lastname: Garcia
authors.forename: Norbert W
authors.initials: NW
authors.lastname: Lutz
authors.forename: Sylviane
authors.initials: S
authors.lastname: Confort-Gouny
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Cozzone
authors.forename: Martine
authors.initials: M
authors.lastname: Armand
authors.forename: Monique
authors.initials: M
authors.lastname: Bernard
mesh_terms: D000818:Animals; D002162:Camelus; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008892:Milk / Q000737:chemistry* / Q000378:metabolism; D008895:Milk, Human / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*
chemical_list: D010743:Phospholipids