title: Correlations of podocyte injury with glucose regulated protein 78 expression and proteinuria in patients with diabetic nephropathy.
abstract: OBJECTIVE
  To explore the podocyte injury in patients with diabetic nephropathy (DN) and analyze its relationship with glucose regulated protein 78 (GRP78) and proteinuria.
  
  
  METHODS
  The clinical data of 48 patients diagnosed as DN by renal biopsy were reviewed. All patients were divided into two groups according to proteinuria (>3.5 g/d, n=31 and 3.5 g/d, n=17). The density of podocytes was illustrated by immunohistochemistry staining of Wilms tumor-1 (WT-1), and the immunofluorescence double-staining results of synaptopodin and GRP78 in podocytes were detected.
  
  
  RESULTS
  The podocyte dentistry of urine protein > 3.5 g/d group was significantly lower than that of urine protein>3.5 g/d group urine protein<3.5 g/d group(P=0.003), and it was negatively correlated with proteinuria (P=0.005). The expressions of synaptopodin and GRP78 in podocytes were also negatively correlated with proteinuria (P=0.004 and P=0.001).
  
  
  CONCLUSIONS
  The podocyte injury is aggravated with increased proteinuria in DN patients, along with the decrease of the adaptive ability of endoplasmic reticulum to stress.
authors.affiliation: Department of Nephrology, PUMC Hospital, CAMS and PUMC, Beijing, China.
authors.forename: Ying-jiu
authors.initials: YJ
authors.lastname: Liu
authors.forename: Yu-bing
authors.initials: YB
authors.lastname: Wen
authors.forename: Jian-ling
authors.initials: JL
authors.lastname: Tao
authors.forename: Jin-hong
authors.initials: JH
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.forename: Wei
authors.initials: W
authors.lastname: Ye
authors.forename: Hang
authors.initials: H
authors.lastname: Li
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Li
authors.forename: Xue-wang
authors.initials: XW
authors.lastname: Li
mesh_terms: D000328:Adult; D003928:Diabetic Nephropathies / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D050199:Podocytes / Q000473:pathology*; D011507:Proteinuria / Q000209:etiology*
chemical_list: D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins