title: Easy fabrication of thin membranes with through holes. Application to protein patterning.
abstract: Since protein patterning on 2D surfaces has emerged as an important tool in cell biology, the development of easy patterning methods has gained importance in biology labs. In this paper we present a simple, rapid and reliable technique to fabricate thin layers of UV curable polymer with through holes. These membranes are as easy to fabricate as microcontact printing stamps and can be readily used for stencil patterning. We show how this microfabrication scheme allows highly reproducible and highly homogeneous protein patterning with micron sized resolution on surfaces as large as 10 cm(2). Using these stencils, fragile proteins were patterned without loss of function in a fully hydrated state. We further demonstrate how intricate patterns of multiple proteins can be achieved by stacking the stencil membranes. We termed this approach microserigraphy.
authors.affiliation: MechanoBiology Institute of Singapore, Singapore, Singapore.
authors.forename: Thomas
authors.initials: T
authors.lastname: Masters
authors.forename: Wilfried
authors.initials: W
authors.lastname: Engl
authors.forename: Zhe L
authors.initials: ZL
authors.lastname: Weng
authors.forename: Bakya
authors.initials: B
authors.lastname: Arasi
authors.forename: Nils
authors.initials: N
authors.lastname: Gauthier
authors.forename: Virgile
authors.initials: V
authors.lastname: Viasnoff
mesh_terms: D000818:Animals; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D055616:Microtechnology / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D014466:Ultraviolet Rays
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; D011506:Proteins