title: Loss of fragile histidine triad and amplification of 1p36.22 and 11p15.5 in primary gastric adenocarcinomas.
abstract: OBJECTIVE
  To investigate the genomic copy number alterations that may harbor key driver genes in gastric tumorigenesis.
  
  
  METHODS
  Using high-resolution array comparative genomic hybridization (CGH), we investigated the genomic alterations of 20 advanced primary gastric adenocarcinomas (seventeen tubular and three mucinous) of Chinese patients from the Jilin province. Ten matching adjacent normal regions from the same patients were also studied.
  
  
  RESULTS
  The most frequent imbalances detected in these cancer samples were gains of 3q26.31-q27.2, 5p, 8q, 11p, 18p, 19q and 20q and losses of 3p, 4p, 18q and 21q. The use of high-resolution array CGH increased the resolution and sensitivity of the observed genomic changes and identified focal genetic imbalances, which included 54 gains and 16 losses that were smaller than 1 Mb in size. The most interesting focal imbalances were the intergenic loss/homozygous deletion of the fragile histidine triad gene and the amplicons 11q13, 18q11.2 and 19q12, as well as the novel amplicons 1p36.22 and 11p15.5.
  
  
  CONCLUSIONS
  These regions, especially the focal amplicons, may harbor key driver genes that will serve as biomarkers for either the diagnosis or the prognosis of gastric cancer, and therefore, a large-scale investigation is recommended.
authors.affiliation: Department of Internal Medicine, The First Teaching Hospital of Jilin University, Changchun 130021, Jilin Province, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liu
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Chen
authors.forename: Man-Li
authors.initials: ML
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Tian
authors.forename: Shibo
authors.initials: S
authors.lastname: Li
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Lee
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000230:Adenocarcinoma / Q000208:ethnology / Q000235:genetics*; D000368:Aged; D022981:Allelic Imbalance / Q000235:genetics; D044466:Asian People / Q000235:genetics; D002681:China; D055028:Comparative Genomic Hybridization; D005260:Female; D005784:Gene Amplification / Q000235:genetics*; D017353:Gene Deletion; D018628:Gene Dosage / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D013274:Stomach Neoplasms / Q000208:ethnology / Q000235:genetics*; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase