title: Expression of CD20 reveals a new store-operated calcium entry modulator in skeletal muscle.
abstract: Among the scarce available data about the biological role of the membrane protein CD20, there is some evidence that this protein functions as a store-operated Ca(2+) channel and/or regulates transmembrane Ca(2+) trafficking. Recent findings indicate that store-operated Ca(2+) entry (SOCE) plays a central role in skeletal muscle function and development, but there remain a number of unresolved issues relating to SOCE modulation in this tissue. Here we describe CD20 expression in skeletal muscle, verifying its membrane localization in myoblasts and adult muscle fibers. Additionally, we show that inhibition of CD20 through antibody binding or gene silencing resulted in specific impairment of SOCE in C2C12 myoblasts. Our results provide novel insights into the CD20 expression pattern, and suggest that functional CD20 is required for SOCE to consistently occur in C2C12 myoblasts. These findings may contribute to future identification of mechanisms and molecules involved in the fine regulation of store-operated Ca(2+) entry in skeletal muscle.
authors.affiliation: Stem Cell Laboratory, Dept. of Pathophysiology and Transplantation, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, Università degli Studi di Milano, via F. Sforza 35, 20122 Milan, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Parolini
authors.forename: Letizia
authors.initials: L
authors.lastname: Cassinelli
authors.forename: Paola
authors.initials: P
authors.lastname: Razini
authors.forename: Clementina
authors.initials: C
authors.lastname: Sitzia
authors.forename: Noemi
authors.initials: N
authors.lastname: Tonna
authors.forename: Silvia
authors.initials: S
authors.lastname: Erratico
authors.forename: Federica
authors.initials: F
authors.lastname: Colleoni
authors.forename: Valentina
authors.initials: V
authors.lastname: Angeloni
authors.forename: Elisa
authors.initials: E
authors.lastname: Maffioli
authors.forename: Andrea
authors.initials: A
authors.lastname: Farini
authors.forename: Simona
authors.initials: S
authors.lastname: Maciotta
authors.forename: Laura
authors.initials: L
authors.lastname: Porretti
authors.forename: Marzia
authors.initials: M
authors.lastname: Belicchi
authors.forename: Fabio
authors.initials: F
authors.lastname: Bianco
authors.forename: Gabriella
authors.initials: G
authors.lastname: Tedeschi
authors.forename: Mirella
authors.initials: M
authors.lastname: Meregalli
authors.forename: Yvan
authors.initials: Y
authors.lastname: Torrente
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D018951:Antigens, CD20 / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D008969:Molecular Sequence Data; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism; D032446:Myoblasts / Q000378:metabolism; D034622:RNA Interference
chemical_list: D000906:Antibodies; D018951:Antigens, CD20