title: Interleukin-22 protects rat PC12 pheochromocytoma cells from serum deprivation-induced cell death.
abstract: Interleukin-22 (IL-22), an IL-10 family cytokine, mediates the crosstalk between leukocytes and epithelial cells. Previous studies reported that IL-22 expresses in mouse brain, and the rat PC12 cells are responsive to IL-22 stimulation. However, the biological roles of IL-22 in neuronal cells remain largely unknown. We show here that IL-22 activates Stat3, p38 mitogen-activated protein kinases (MAPK), and Akt pathways and inhibits Erk/MAPK pathway in naïve PC12 cells. We further demonstrate that IL-22 protects naïve PC12 cells from serum starvation-induced cell death via the Jak1/Stat3 and Akt pathways. We also show that IL-22 has no effects on naïve PC12 cell proliferation and cannot protect naïve PC12 cells from 1-methyl-4-phenylpyridinium (MPP(+))-induced cytotoxicity. However, IL-22 exerts a dose-dependent protective effect on MPP(+)-induced neurodegeneration in nerve growth factor-differentiated PC12 cells. Overall, our data suggest that IL-22 might play a role in neurological processes. To our knowledge, this is the first report showing that IL-22 confers a neuroprotective function, which may provide a new therapeutic option for treatment of neurodegenerative diseases.
authors.affiliation: Northern Jiangsu People's Hospital, Nantong West Road No. 98, Yangzhou 225001, China.
authors.forename: Yongchun
authors.initials: Y
authors.lastname: Liu
authors.forename: Wenyan
authors.initials: W
authors.lastname: Pan
authors.forename: Shengmei
authors.initials: S
authors.lastname: Yang
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wu
authors.forename: Jianfu
authors.initials: J
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.forename: Zengqiang
authors.initials: Z
authors.lastname: Yuan
authors.forename: Songshu
authors.initials: S
authors.lastname: Meng
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology; D000818:Animals; D016923:Cell Death; D002454:Cell Differentiation; D049109:Cell Proliferation; D002470:Cell Survival; D016895:Culture Media, Serum-Free; D007378:Interleukins / Q000378:metabolism* / Q000494:pharmacology; D053613:Janus Kinase 1 / Q000378:metabolism; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D050796:STAT3 Transcription Factor / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D000097043:Interleukin-22
chemical_list: D015655:1-Methyl-4-phenylpyridinium; D016895:Culture Media, Serum-Free; D007378:Interleukins; D053613:Janus Kinase 1; D051057:Proto-Oncogene Proteins c-akt; D050796:STAT3 Transcription Factor; D048051:p38 Mitogen-Activated Protein Kinases; D000097043:Interleukin-22