title: Maintenance and characterization of multipotent mesenchymal stem cells isolated from canine umbilical cord matrix by collagenase digestion.
abstract: Multipotent mesenchymal stem cells have been considered as a novel clinical approach for cell therapy and regenerative medicine. In this study, mesenchymal stem cells (MSCs) were successfully isolated from canine umbilical cord matrix (cUCM; also referred to as Wharton's Jelly) by collagenase digestion and further characterized for multipotent properties associated with MSCs. Our cUCM-derived MSCs (cUCM-MSCs) were plastic adherent, spindle-shaped and fibroblast-like cells, maintaining expression of pluripotency markers such as Oct3/4, Nanog, Sox-2 and SSEA-4 as well as normal chromosomal number during a long-term proliferative culture. The cells expressed MSCs-specific surface markers, including CD44, CD90, CD105, and CD184, but did not CD29, CD33, CD34, and CD45. More importantly, cUCM-MSCs could differentiate into mesodermal (adipocyte, osteocyte and chondrocyte) and ectodermal (neuronal cell) cell lineages. These results imply that collagenase digestion would be a highly effective way to isolate multipotent MSCs in abundant amounts.
authors.affiliation: Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 6-dong, Anyang-si, Gyeonggi-do 430-757, Republic of Korea.
authors.forename: K S
authors.initials: KS
authors.lastname: Lee
authors.forename: J-J
authors.initials: JJ
authors.lastname: Nah
authors.forename: B-C
authors.initials: BC
authors.lastname: Lee
authors.forename: H T
authors.initials: HT
authors.lastname: Lee
authors.forename: H-S
authors.initials: HS
authors.lastname: Lee
authors.forename: B-J
authors.initials: BJ
authors.lastname: So
authors.forename: S-H
authors.initials: SH
authors.lastname: Cha
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D017364:Collagenases; D004396:Coloring Agents; D004285:Dogs; D005434:Flow Cytometry / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D020869:Gene Expression Profiling / Q000662:veterinary; D066298:In Vitro Techniques; D007621:Karyotyping / Q000662:veterinary; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D004396:Coloring Agents; D017364:Collagenases