title: Ammonia-oxidizing bacteria rather than ammonia-oxidizing archaea were widely distributed in animal manure composts from field-scale facilities.
abstract: The distribution of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in cattle, swine, and chicken manure compost was analyzed. PCR-denaturing gradient gel electrophoresis (DGGE) showed that a Candidatus Nitrososphaera gargensis-like sequence dominated in cattle manure compost, while few AOA were detected in other composts. In the case of AOB, Nitrosomonas-like sequences were detected with higher diversity in cattle and swine manure composts. The relative abundance of ammonia oxidizers by real-time PCR revealed that more AOB was present in compost except in one swine manure compost. Our results indicated that AOB rather than AOA are widely distributed in animal manure compost.
authors.affiliation: Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, 232–3 Yomogida, Naruko-onsen, Osaki, Miyagi 989–6711, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Ryu
authors.initials: R
authors.lastname: Oishi
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Suyama
authors.forename: Chika
authors.initials: C
authors.lastname: Tada
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakai
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D001105:Archaea / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D002645:Chickens; D019641:DNA, Archaeal / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D008372:Manure / Q000382:microbiology*; D058465:Nitrification; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013552:Swine
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D008372:Manure; D012336:RNA, Ribosomal, 16S; D000641:Ammonia