title: Reduction of 13 kD prolamins increases recombinant protein yield and recovery rate in rice endosperm.
abstract: RNA silencing inducible sequence (RSIS) causes post-transcriptional gene silencing (PTGS) of 5' or 3' flanking sequence-containing genes by inhibiting proper transcriptional termination. Exploiting this nature, 13 kD Pro-less, in which major seed storage protein (SSP) 13 kD prolamins are reduced, has been generated. In 13 kD Pro-less, other SSPs, such as glutelins, are increased as a compensation effect to maintain amino acid pool. 7Crp is the seven-linked epitope peptide derived from major cedar pollen allergens Cry j 1 and Cry j 2. When 7Crp is expressed in 13 kD Pro-less endosperm, accumulation level of 7Crp increased. Furthermore, recovery rate of 7Crp without reducing reagent increased. These findings indicate that 13 kD Pro-less endosperm provides a good production platform for recombinant proteins.
authors.affiliation: Genetically Modified Organism Research Center, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Taiji
authors.initials: T
authors.lastname: Kawakatsu
authors.forename: Fumio
authors.initials: F
authors.lastname: Takaiwa
mesh_terms: D056625:Endosperm / Q000378:metabolism*; D005983:Glutens / Q000235:genetics / Q000378:metabolism; D012275:Oryza / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D055315:Prolamins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D055315:Prolamins; D011994:Recombinant Proteins; D005983:Glutens