title: [Isolation, purification and characterization of endothelial cells from endometrial cancer and normal endometrial tissue].
abstract: OBJECTIVE
  To immunopurify human endometrial endothelial cells (HEEC) from fresh surgical specimens of endometrial cancers and normal endometrial tissues, and investigate their biological characteristics.
  
  
  METHODS
  Endothelial cells of endometrial cancers and normal endometrial tissues were isolated using anti-CD31 conjugated magnetic microbeads. The isolated endothelial cells were cultured in vitro and their origins were identified. Their angiogenic characteristics were observed by MTT, wound healing, Transwell cell invasion and tube formation assays.
  
  
  RESULTS
  Flow cytometry revealed that the immunopurification technique yielded endothelial cell purity of > 95% in all samples. All purified HEEC were characterized as endothelial cells on the basis of expression of the classical endothelial markers vWF and CD31 as shown by immunofluorescence examination. Although the tumor-associated HEEC didn't show more rapid proliferation than normal HEEC, they exhibited enhanced migration ability (P = 0.006), potent invasiveness (P = 0.033), and elevated tube formation in vitro (P = 0.029).
  
  
  CONCLUSIONS
  Human endometrial endothelial cells can be efficiently isolated from endometrial cancer and normal endometrial tissues by immunomagnetic methods. Tumor-associated HEEC exhibit enhanced migratory ability, potent invasiveness, and elevated tube formation in vitro.
authors.affiliation: Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan 250117, China.
authors.forename: Xue-lian
authors.initials: XL
authors.lastname: Du
authors.forename: Xiu-gui
authors.initials: XG
authors.lastname: Sheng
authors.forename: Chun-xiao
authors.initials: CX
authors.lastname: Yan
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Lu
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Qing-shui
authors.initials: QS
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D016889:Endometrial Neoplasms / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014841:von Willebrand Factor