title: PFGE and AFLP genotyping of Staphylococcus aureus subsp. anaerobius isolated from goats with Morel's disease.
abstract: Staphylococcus aureus subsp. anaerobius is the etiological agent of the Morel's disease in sheep and goats. The disease presents with subcutaneous abscesses, located mainly in the superficial lymph nodes. Forty-one isolates of S. aureus subsp. anaerobius were collected from two outbreaks of the Morel's disease in Poland in years 2006-2008. Analysis of DNA SmaI digests by PFGE showed that 35 of 41 isolates belonged to the same PFGE type, identical to the type strain of S. aureus subsp. anaerobius ATCC 35844, confirming high level of clonality of the species. The DNA patterns of the remaining identical 6 isolates, different from the reference strain only by two bands, were found closely related. Genotyping performed with AFLP technique revealed two clonal groups including 16 and 25 isolates, respectively. The study indicated that AFLP technique might be a better discriminatory tool for genetic analysis of S. aureus subsp. anaerobius isolates, when compared to PFGE.
authors.affiliation: Department of Small Animal Disease with Clinic, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland. malaolga@gmail.com
authors.forename: O
authors.initials: O
authors.lastname: Szaluś-Jordanow
authors.forename: D
authors.initials: D
authors.lastname: Chrobak
authors.forename: M
authors.initials: M
authors.lastname: Pyrgiel
authors.forename: A
authors.initials: A
authors.lastname: Lutyńska
authors.forename: J
authors.initials: J
authors.lastname: Kaba
authors.forename: M
authors.initials: M
authors.lastname: Czopowicz
authors.forename: L
authors.initials: L
authors.lastname: Witkowski
authors.forename: M
authors.initials: M
authors.lastname: Kizerwetter-Świda
authors.forename: M
authors.initials: M
authors.lastname: Binek
authors.forename: T
authors.initials: T
authors.lastname: Frymus
mesh_terms: D000038:Abscess / Q000382:microbiology / Q000662:veterinary*; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D010802:Phylogeny; D011044:Poland; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification