title: LNA real-time PCR probe quantification of hepatitis B virus DNA.
abstract: In the present study, we standardized a TaqMan locked nucleic acid (LNA) real-time polymerase chain reaction (PCR) probe for the accurate quantification and detection of hepatitis B virus (HBV) DNA in serum (plasma), and evaluated its methodology. LNA probe technology had a much better detection performance in HBV DNA than the common TaqMan probe. The assay based on the LNA probe had a wider linear detection range, higher sensitivity, stability and amplification efficiency, and a lower concentration of probes than the TaqMan probe. Among the 15 cases with chronic hepatitis B surface antigen (HBsAg) (+) alone, only 4 cases that were detected by TaqMan real-time PCR were negative; however, the same samples were positive by LNA real-time PCR (p<0.05). A positive correlation between viral load measurements for the 35 samples with HBV-positive DNA was detected in both LNA and TaqMan real-time PCR.
authors.affiliation: Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University Medical College;
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Xueqian
authors.initials: X
authors.lastname: Wang
authors.forename: Junhua
authors.initials: J
authors.lastname: Zhang
authors.forename: Guanghui
authors.initials: G
authors.lastname: Song