title: [Genetic association between interleukin-10 promoter microsatellite polymorphisms and hepatitis B virus infection in Yi, Yao and Han ethnic populations of Guizhou province].
abstract: OBJECTIVE
  To investigate the association between interleukin-10 (IL-10) gene promoter microsatellite polymorphisms and the susceptibility to hepatitis B virus infection in Han, Yi and Yao ethnicities in GuiZhou province.
  
  
  METHODS
  500 volunteers were selected from Guizhou province. Allelic frequency of IL-10.G and IL-10.R loci was identified by short tandom repeat polymerase chain reaction. The relativity between allelic frequency and HBV infection was analyzed.
  
  
  RESULTS
  Genotype data from H-W analysis on all the IL-10 polymorphisms indicated that it was a random distribution. Very high HBV infection rates were found in the native ethnic minorities of Guizhou province. The overall HBV infection rate among the total population was 67.00%, with the HBV infection rates of Yi nationality in Weining, Yi nationality in Qianxi, Yao nationality in Libo and Han nationality in Libo as 51.85%, 42.86%, 79.52% and 84.30%, respectively. The polymorphisms distribution of IL-10.G and IL-10.R were statistically different among the ethnic groups (P < 0.05). The polymorphisms distribution of IL-10.R had no significant difference between HBV infection group and non-infection group, as well as among HBV natural removal group and non-infected group in all the ethnic groups. The frequency of IL-10.G 459 bp (19CA) was significantly higher in non-infection group than in the infected group (P < 0.05). The frequency of IL-10.G 471 bp (25CA) was significantly higher in the non-infection group than in the HBV natural removal group (P < 0.05). The polymorphisms distribution of IL-10.G did not show significant difference between the HBV infection group and the HBV natural removal group in all the ethnic groups. We did not find any differences in allelic and genotypic frequencies of IL-10.G between infection group and non-infection group in Yi nationality in Weining, and Yao nationality in Libo (P > 0.05), as well as HBV natural removal group and non-infected group (P > 0.05).
  
  
  CONCLUSIONS
  The polymorphisms distribution of IL-10.R and IL-10.G did not show significant difference in Yi, Yao and Han ethnics population living in Guizhou province. IL-10.G seemed to influence the susceptibility of HBV infection in Han, Yao and Yi ethnics population of Guizhou province.
authors.affiliation: Key Laboratory of Biochemistry and Molecular Biology, Guiyang Medical University, Guiyang 550004, China.
authors.forename: Chan-Juan
authors.initials: CJ
authors.lastname: Wang
authors.forename: Ke-Ren
authors.initials: KR
authors.lastname: Shan
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Chang-Xue
authors.initials: CX
authors.lastname: Wu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xie
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Qi
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Guan
mesh_terms: D000483:Alleles; D005006:Ethnicity / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000208:ethnology*; D005838:Genotype; D006509:Hepatitis B / Q000208:ethnology* / Q000235:genetics; D006515:Hepatitis B virus; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D016753:Interleukin-10