title: TAT-Apoptin induces apoptosis in the human bladder cancer EJ cell line and regulates Bax, Bcl-2, caspase-3 and survivin expression.
abstract: In order to identify the antitumor effect of TAT-Apoptin on the human bladder cancer EJ cell line and study its impact on the expression of the apoptosis-related genes bax, bcl-2, caspase-3 and survivin, the MTT assay, real-time quantitative PCR and western blot analysis were used in this study. The results of the MTT assay indicated that TAT-Apoptin was able to inhibit the proliferation of EJ cells in a dose-dependent manner. The expression of Bax, Bcl-2, Caspase-3 and Survivin mRNA and protein following the treatment of the EJ cells with TAT-Apoptin (0.1, 0.5, 1, 10, 50 and 100 μg/ml) for 24, 48 and 72 h was analyzed. Cell proliferation was significantly different after treatment with the various concentrations of TAT-Apoptin and the durations of treatment. The level of expression of Bcl-2 and Survivin in EJ cells decreased significantly, while that of Bax and Caspase-3 increased significantly at the mRNA and protein levels.
authors.affiliation: Department of Nephrology, The First Affiliated Hospital, Kunming Medical University, Kunming 650031;
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ma
authors.forename: Wenxing
authors.initials: W
authors.lastname: Fan
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Li
authors.forename: Bingbing
authors.initials: B
authors.lastname: Han
authors.forename: Zhijia
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jiansong
authors.initials: J
authors.lastname: Wang