title: Treatment of adriamycin-induced nephropathy with erythropoietin and G-CSF.
abstract: BACKGROUND
  Granulocyte colony-stimulating factor (G-CSF) and Erythropoietin (EPO) are known to stimulate the growth and differentiation of progenitor cells to prevent acute renal injury. This study aimed to assess the use of growth factors to mobilize stem cell in a mouse model of adriamycin-induced chronic kidney disease.
  
  
  METHODS
  All animals were injected with adriamycin for kidney injury and allocated into three treatment groups (G-CSF, EPO and G-CSF + EPO), and a control group (adriamycin alone).
  
  
  RESULTS
  Number of atrophic sites, glomerulosclerosis rate and interstitial fibrosis severity score were assessed in all groups. In all treatment groups, histologic parameters did not significantly differ, but were lower than in the control group (P<.001). Scal and CD34 expressions among treatment groups showed no statistically significant difference, but were higher than in the control group (P<.0001). CD105 expression was higher in EPO and G+EPO as compared to G-CSF and the control group (P<.0001), with no statistically significant difference between the latter two groups (P = NS).
  
  
  CONCLUSIONS
  G-CSF and EPO had a histologic protective effect, while treatment with EPO + G-CSF had no additive effects in a model of adriamycin-induced chronic kidney disease.
authors.affiliation: Department of Internal Medicine-UNESP, Universidade Estadual Paulista, Botucatu, Sao Paulo, Brazil. landrade@fmb.unesp.br
authors.forename: Luis Gustavo
authors.initials: LG
authors.lastname: Modelli de Andrade
authors.forename: Rosa Marlene
authors.initials: RM
authors.lastname: Viero
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Cordeiro de Carvalho
mesh_terms: D000818:Animals; D004317:Doxorubicin / Q000008:administration & dosage; D004921:Erythropoietin / Q000627:therapeutic use*; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D007674:Kidney Diseases / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D004317:Doxorubicin