title: Xenopus transgenics: methods using transposons.
abstract: The generation of transgenic animals is an essential tool for many genetic strategies. DNA "cut-and-paste" transposon systems can be used to efficiently modify the Xenopus genome. The DNA transposon substrate, harbored on a circularized plasmid, is co-injected into fertilized Xenopus embryos at the one-cell stage together with mRNA encoding the cognate transposase enzyme. The cellular machinery rapidly translates the exogenous mRNA to produce active transposase enzyme that catalyzes excision of the transposon substrate from the plasmid and stable integration into the genomic DNA.
authors.affiliation: Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN, USA.
authors.forename: Clair M
authors.initials: CM
authors.lastname: Kelley
authors.forename: Donald A
authors.initials: DA
authors.lastname: Yergeau
authors.forename: Haiqing
authors.initials: H
authors.lastname: Zhu
authors.forename: Emin
authors.initials: E
authors.lastname: Kuliyev
authors.forename: Paul E
authors.initials: PE
authors.lastname: Mead
mesh_terms: D000821:Animal Feed; D000822:Animal Husbandry; D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D046508:Culture Techniques; D004251:DNA Transposable Elements / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology; D005260:Female; D008297:Male; D008845:Microinjections; D016254:Mutagenesis, Insertional; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D012102:Reproductive Control Agents / Q000008:administration & dosage; D014981:Xenopus / Q000235:genetics*
chemical_list: D006063:Chorionic Gonadotropin; D004251:DNA Transposable Elements; D012333:RNA, Messenger; D012102:Reproductive Control Agents