title: Estradiol enhances effects of fructose rich diet on cardiac fatty acid transporter CD36 and triglycerides accumulation.
abstract: Fructose rich diet increases hepatic triglycerides production and has deleterious cardiac effects. Estrogens are involved in regulation of lipid metabolism as well, but their effects are cardio beneficial. In order to study effects of fructose rich diet on the main heart fatty acid transporter CD36 and the role of estrogens, we subjected ovariectomized female rats to the standard diet or fructose rich diet, with or without estradiol (E2) replacement. The following parameters were analyzed: feeding behavior, visceral adipose tissue mass, plasma lipids, cardiac CD36 expression, localization and insulin regulation, as well as the profile of cardiac lipids. Results show that fructose rich diet significantly increased plasma triglycerides and decreased plasma free fatty acid (FFA) concentration, while E2 additionally emphasized FFA decrease. The fructose diet increased cardiac plasma membrane content of CD36 in the basal and insulin-stimulated states, and decreased its low density microsomes content. The E2 in fructose-fed rats raised the total cardiac protein content of CD36, its presence in plasma membranes and low density microsomes, and cardiac deposition of triglycerides, as well. Although E2 counteracts fructose in some aspects of lipid metabolism, and separately they have opposite cardiac effects, in combination with fructose rich diet, E2 additionally enhances CD36 presence in plasma membranes of cardiac cells and triglycerides accumulation, which paradoxically might promote deleterious effects of fructose diet on cardiac lipid metabolism. Taken together, the results presented in this work are of high importance for clinical administration of estrogens in females with a history of type 2 diabetes.
authors.affiliation: Laboratory for Molecular Biology and Endocrinology, Vinča Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia. gogi@vinca.rs
authors.forename: Goran
authors.initials: G
authors.lastname: Korićanac
authors.forename: Snežana
authors.initials: S
authors.lastname: Tepavčević
authors.forename: Snježana
authors.initials: S
authors.lastname: Romić
authors.forename: Maja
authors.initials: M
authors.lastname: Živković
authors.forename: Mojca
authors.initials: M
authors.lastname: Stojiljković
authors.forename: Tijana
authors.initials: T
authors.lastname: Milosavljević
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Stanković
authors.forename: Marijana
authors.initials: M
authors.lastname: Petković
authors.forename: Tina
authors.initials: T
authors.lastname: Kamčeva
authors.forename: Zorica
authors.initials: Z
authors.lastname: Žakula
mesh_terms: D000818:Animals; D018955:CD36 Antigens / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004032:Diet; D004435:Eating / Q000187:drug effects; D002149:Energy Intake / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005632:Fructose / Q000032:analysis*; D006321:Heart / Q000187:drug effects*; D050152:Intra-Abdominal Fat / Q000187:drug effects / Q000378:metabolism; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood / Q000378:metabolism*
chemical_list: D018955:CD36 Antigens; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D005632:Fructose; D004958:Estradiol