title: Screening and quantitative analysis of antioxidants in the fruits of Livistona chinensis R. Br using HPLC-DAD-ESI/MS coupled with pre-column DPPH assay.
abstract: In this study, a high performance liquid chromatography-photo diode array detection-electrospray ionization tandem mass spectrometry (HPLC-DAD/ESI-MS) with pre-column DPPH assay is developed for screening the antioxidant components in the fruits of Livistona chinensis R. Br. Accordingly, six antioxidative flavonoids are identified as orientin, isoorientin, vitexin, isovitexin, isorhamnetin-3-O-glucoside and tricin in methanolic extract of L. chinensis fruits, based on their mass spectra and fragmentation patterns. To the best of our knowledge, orientin, isoorientin, isovitexin and isorhamnetin-3-O-glucoside were found firstly in this plant. The free radical scavenging activity of the six antioxidants found is further examined by off-line DPPH assay. The results indicated that the free radical scavenging activity of orientin and isoorientin are stronger than those of two antioxidative drugs, vitamin C and baicalin. In addition, an HPLC-DAD method is firstly established for simultaneous determination of the six antioxidants in L. chinensis fruits. Tricin was found to be the major component in L. chinensis fruits.
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350004, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yao
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Peiying
authors.initials: P
authors.lastname: Shi
authors.forename: Juan
authors.initials: J
authors.lastname: Hu
authors.forename: Shaoguang
authors.initials: S
authors.lastname: Li
authors.forename: Liying
authors.initials: L
authors.lastname: Huang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Lin
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lin
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D028023:Arecaceae / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D010851:Picrates / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods*
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl