title: Epidemiology of Staphylococcus aureus in pigs and farmers in the largest farm in Dakar, Senegal.
abstract: Between December 2009 and November 2011, we collected 57 (12.3%) Staphylococcus aureus isolates from 464 pigs and 16 (30.8%) isolates from 52 farmers in the largest farm in Dakar. Fifty-one isolates (70%) belonged to four major multilocus sequence typing clonal complexes (CCs): CC152 (26.0%), CC15 (19.2%), CC5 (13.7%), and CC97 (10.9%). The CC variability among the pigs was similar to that observed among the farmers. Six isolates that were recovered only among pigs were resistant to methicillin (10.5%). They were assigned to the ST5-staphylococcal cassette chromosome mec type (SCCmec) IV (n = 5) and ST88-SCCmec IV (n = 1) clones. The luk-PV genes encoding Panton-Valentine leukocidin (PVL), present in 43 (58.9%) isolates overall, including all major CCs and the MRSA ST5-SCCmec IV clone, were highly prevalent compared to data from industrialized countries. This finding is of major concern with regard to the potential virulence of these strains.
authors.affiliation: Unit of Medical Biology and Environment, Institut Pasteur, Dakar, Senegal.
authors.forename: Cheikh
authors.initials: C
authors.lastname: Fall
authors.forename: Abdoulaye
authors.initials: A
authors.lastname: Seck
authors.forename: Vincent
authors.initials: V
authors.lastname: Richard
authors.forename: Moustapha
authors.initials: M
authors.lastname: Ndour
authors.forename: Mbacke
authors.initials: M
authors.lastname: Sembene
authors.forename: Frederic
authors.initials: F
authors.lastname: Laurent
authors.forename: Sebastien
authors.initials: S
authors.lastname: Breurec
mesh_terms: D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005098:Exotoxins / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005838:Genotype; D006801:Humans; D007956:Leukocidins / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D008712:Methicillin / Q000494:pharmacology; D016106:Methicillin Resistance; D008875:Middle Aged; D058885:Multilocus Sequence Typing / Q000662:veterinary; D046915:Penicillin-Binding Proteins; D012675:Senegal / Q000453:epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D013552:Swine / Q000382:microbiology*; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus; D008712:Methicillin