title: Liquid chromatography-tandem mass spectrometry method for simultaneous determination of seven commonly used anticancer drugs in human plasma.
abstract: This paper describes the development and validation of a novel, general liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of cyclophosphamide, ifosfamide, irinotecan, etoposide, gemcitabine, carboplatin and pemetrexed concentrations in human plasma. Samples were prepared by two kinds of extraction method and analyzed using a gradient separation over an Atlantis T3-C18 column (2.1 mm×100 mm, 3 μm, Waters). Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile-water (0.1% formic acid and 10mM ammonium acetate) at a flow rate of 0.25 mL/min. Linear coefficients of correlation were >0.992 for all analytes. The intra- and inter-day relative standard deviation across three validation runs over the entire concentration range was less than 9.2%, while the accuracy was within ± 10.5%. The mean recovery of all the analytes ranged from 50.0 to 81.0%. This method was successfully applied to clinical samples from cancer patients.
authors.affiliation: Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, PR China.
authors.forename: Jingya
authors.initials: J
authors.lastname: Zhou
authors.forename: Shouhong
authors.initials: S
authors.lastname: Gao
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhan
authors.forename: Fei
authors.initials: F
authors.lastname: Cai
authors.forename: Jingxian
authors.initials: J
authors.lastname: Li
authors.forename: Wansheng
authors.initials: W
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000097:blood* / Q000627:therapeutic use; D002853:Chromatography, Liquid / Q000379:methods*; D016903:Drug Monitoring; D004355:Drug Stability; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents