title: Electroneutral silica-based hybrid monolith for hydrophilic interaction capillary electrochromatography.
abstract: A novel electroneutral and polar silica-based hybrid monolith was developed by an in situ copolymerization of 2-hydroxyethylmethacrylate (HEMA) and polyhedral oligomeric silsesquioxane methacryl substituted (POSS-MA), and successfully employed for hydrophilic interaction capillary electrochromatography (HI-CEC). A good mechanical stability of the prepared monolith was gained with the permeability decreasing from 6.52×10(-14) m2 to 4.61×10(-14) m2 when mobile phase changed from ACN to water. A significant cathodal EOF was obtained through attracting ions from the mobile phase despite the fact that it was devoid of ionizable functional groups on the surface of stationary phases, and a typical HI-CEC mechanism was achieved. The morphologies of the hybrid silica-based monolithic matrixes were observed, and the performance of this silica-based hybrid monolith was also investigated. Satisfactory column performances were carried out for both the neutral and charged analytes with HI-CEC. The analytes including uncharged amides and phenols, charged nucleic acid bases and nucleosides and enkephalins, were well separated with good peak symmetry. High separation efficiencies of charged alkaloids and enkephalins could get up to 145,000 plates/m and 75,000 plates/m, respectively.
authors.affiliation: Institute of Food Safety and Environmental Monitoring, Fuzhou University, Fuzhou 350108, China. xulin@fzu.edu.cn
authors.forename: Xucong
authors.initials: X
authors.lastname: Lin
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.forename: Yanqiong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Liu
authors.forename: Zenghong
authors.initials: Z
authors.lastname: Xie
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D004745:Enkephalins / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry*; D010316:Particle Size; D011073:Polyamines / Q000737:chemistry; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives / Q000737:chemistry; D016062:Porosity; D015203:Reproducibility of Results
chemical_list: D000470:Alkaloids; D004745:Enkephalins; D008689:Methacrylates; D017646:Organosilicon Compounds; D011073:Polyamines; C056971:poly(2-hydroxyethyl methacrylate)-polyamine graft copolymer; C503161:polyhedral oligomeric silsesquioxane methacrylate; D011102:Polyhydroxyethyl Methacrylate