title: Elucidation of the biosynthesis of the di-C-glycosylflavone isoschaftoside, an allelopathic component from Desmodium spp. that inhibits Striga spp. development.
abstract: Isoschaftoside, an allelopathic di-C-glycosylflavone from Desmodium spp. root exudates, is biosynthesised through sequential glucosylation and arabinosylation of 2-hydroxynaringenin with UDP-glucose and UDP-arabinose. Complete conversion to the flavone requires chemical dehydration implying a dehydratase enzyme has a role in vivo to complete the biosynthesis. The C-glucosyltransferase has been partially characterised and its activity demonstrated in highly purified fractions.
authors.affiliation: Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK.
authors.forename: Mary L
authors.initials: ML
authors.lastname: Hamilton
authors.forename: Serge P
authors.initials: SP
authors.lastname: Kuate
authors.forename: Melissa
authors.initials: M
authors.lastname: Brazier-Hicks
authors.forename: John C
authors.initials: JC
authors.lastname: Caulfield
authors.forename: Ruth
authors.initials: R
authors.lastname: Rose
authors.forename: Robert
authors.initials: R
authors.lastname: Edwards
authors.forename: Baldwyn
authors.initials: B
authors.lastname: Torto
authors.forename: John A
authors.initials: JA
authors.lastname: Pickett
authors.forename: Antony M
authors.initials: AM
authors.lastname: Hooper
mesh_terms: D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D006027:Glycosides / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012639:Seeds / Q000737:chemistry; D034362:Striga / Q000187:drug effects* / Q000254:growth & development; D013696:Temperature
chemical_list: D005419:Flavonoids; D006027:Glycosides; C515112:schaftoside; C515113:isoschaftoside