title: Quantification of furanic compounds in coated deep-fried products simulating normal preparation and consumption: optimisation of HS-SPME analytical conditions by response surface methodology.
abstract: The validation of a method for the simultaneous quantification of furanic compounds in coated deep-fried samples processed and handled as usually consumed is presented. The deep-fried food was grinded using a device that simulates the mastication, and immediately analysed by headspace solid phase microextraction coupled to gas chromatography-mass spectrometry. Parameters affecting the efficiency of HS-SPME procedure were selected by response surface methodology, using a 2(3) full-factorial central composite design. Optimal conditions were achieved using 2g of sample, 3g of NaCl and 40min of absorption time at 37°C. Consistency between predicted and experimented values was observed and quality parameters of the method were established. As a result, furan, 2-furfural, furfuryl alcohol and 2-pentylfuran were, for the first time, simultaneously detected and quantified (5.59, 0.27, 10.48 and 1.77μgg(-1) sample, respectively) in coated deep-fried fish, contributing to a better understanding of the amounts of these compounds in food.
authors.affiliation: REQUIMTE, Laboratório de Bromatologia e Hidrologia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Rua Jorge Viterbo Ferreira, 4050-313 Porto, Portugal. triny@unex.es
authors.forename: T
authors.initials: T
authors.lastname: Pérez-Palacios
authors.forename: C
authors.initials: C
authors.lastname: Petisca
authors.forename: A
authors.initials: A
authors.lastname: Melo
authors.forename: I M P L V O
authors.initials: IM
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D003296:Cooking; D005399:Fishes; D005504:Food Analysis / Q000379:methods*; D005663:Furans / Q000032:analysis* / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D052617:Solid Phase Microextraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D005663:Furans