title: In situ monitoring Alzheimer's disease β-amyloid aggregation and screening of Aβ inhibitors using a perylene probe.
abstract: A cationic perylene tetracarboxylic acid diimide derivative (1) is employed as a probe for in situ monitoring of Aβ aggregation and screening Aβ inhibitors. The assay is based on the fluorescence change through the aggregation of compound 1 following Aβ assembly. Importantly, this probe, compared with the well known amyloid-staining compound thioflavin T (ThT), is more sensitive to Aβ oligomer, which is highly toxic and plays a crucial role in the early stages of Alzheimer's disease.
authors.affiliation: Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Chuanqi
authors.initials: C
authors.lastname: Zhao
authors.forename: Xinjian
authors.initials: X
authors.lastname: Yang
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ren
authors.forename: Can
authors.initials: C
authors.lastname: Xu
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Qu
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors / Q000737:chemistry*; D052160:Benzothiazoles; D002412:Cations; D004277:DNA, Single-Stranded / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D018625:Microscopy, Atomic Force / Q000379:methods; D008956:Models, Chemical; D010446:Peptide Fragments / Q000037:antagonists & inhibitors; D010569:Perylene / Q000737:chemistry*; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D020349:Surface Plasmon Resonance; D013844:Thiazoles / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D002412:Cations; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D013844:Thiazoles; C009462:thioflavin T; D010569:Perylene