title: Molecular and functional analysis of cellular phenomena using single-cell mass spectrometry.
abstract: Single-cell analysis has attracted attention in many fields of biological studies as a tool to survey the precise mechanisms of cellular and molecular behavior. The development of sensitive mass spectrometry allows the study of molecules in single cells or small regions. Matrix-assisted laser desorption/ionization imaging mass spectrometry and secondary-ion mass spectrometry use in situ ionization of specimens on sample plates to visualize molecular distributions as images from mass spectra. Several single-cell mass spectrometry technologies that initially recover a single cell followed by ionization have been developed. Among them, only nanospray-mediated sampling and ionization named Live Single-cell Mass Spectrometry can be used for real-time analysis. This paper explains that method in detail.
authors.affiliation: Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Tsuyama
authors.forename: Hajime
authors.initials: H
authors.lastname: Mizuno
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Masujima
mesh_terms: D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods*