title: Detection of DNA damage by using hairpin molecular beacon probes and graphene oxide.
abstract: A hairpin molecular beacon tagged with carboxyfluorescein in combination with graphene oxide as a quencher reagent was used to detect the DNA damage by chemical reagents. The fluorescence of molecular beacon was quenched sharply by graphene oxide; while in the presence of its complementary DNA the quenching efficiency decreased because their hybridization prevented the strong adsorbability of molecular beacon on graphene oxide. If the complementary DNA was damaged by a chemical reagent and could not form intact duplex structure with molecular beacon, more molecular beacon would adsorb on graphene oxide increasing the quenching efficiency. Thus, damaged DNA could be detected based on different quenching efficiencies afforded by damaged and intact complementary DNA. The damage effects of chlorpyrifos-methyl and three metabolites of styrene such as mandelieaeids, phenylglyoxylieaeids and epoxystyrene on DNA were studied as models. The method for detection of DNA damage was reliable, rapid and simple compared to the biological methods.
authors.affiliation: Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Chemistry and Chemical Engineering, Southeast University, Jiangning District, Nanjing 211189, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Qian
authors.initials: Q
authors.lastname: Lu
authors.forename: Ying
authors.initials: Y
authors.lastname: Tong
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Songqin
authors.initials: S
authors.lastname: Liu
mesh_terms: D002273:Carcinogens / Q000633:toxicity; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004249:DNA Damage; D005452:Fluoresceins / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D055029:Inverted Repeat Sequences; D008958:Models, Molecular; D008968:Molecular Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D002273:Carcinogens; D005452:Fluoresceins; D015345:Oligonucleotide Probes; D010087:Oxides; C024098:6-carboxyfluorescein; D006108:Graphite; D004247:DNA