title: Identification of the TaBTF3 gene in wheat (Triticum aestivum L.) and the effect of its silencing on wheat chloroplast, mitochondria and mesophyll cell development.
abstract: The full-length cDNA (882bp) and DNA (1742bp) sequences encoding a basic transcription factor 3, designated as TaBTF3, were first isolated from common wheat (Triticum aestivum L.). Subcellular localization studies revealed that the TaBTF3 protein was mainly located in the cytoplasm and nucleus. In TaBTF3-silenced transgenic wheat seedlings obtained using the Virus-induced gene silencing (VIGS) method, the chlorophyll pigment content was markedly reduced. However, the malonaldehyde (MDA) and H(2)O(2) contents were enhanced, and the structure of the wheat mesophyll cell was seriously damaged. Furthermore, transcripts of the chloroplast- and mitochondrial-encoded genes were significantly reduced in TaBTF3-silenced transgenic wheat plants. These results suggest that the TaBTF3 gene might function in the development of the wheat chloroplast, mitochondria and mesophyll cell. This paper is the first report to describe the involvement of TaBTF3 in maintaining the normal plant mesophyll cell structure.
authors.affiliation: National Engineering Research Centre for Wheat, The Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Hong-Zhen
authors.initials: HZ
authors.lastname: Ma
authors.forename: Guo-Qin
authors.initials: GQ
authors.lastname: Liu
authors.forename: Cheng-Wei
authors.initials: CW
authors.lastname: Li
authors.forename: Guo-Zhang
authors.initials: GZ
authors.lastname: Kang
authors.forename: Tian-Cai
authors.initials: TC
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D002736:Chloroplasts / Q000235:genetics / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D050259:Genes, Mitochondrial; D058503:Mesophyll Cells / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008928:Mitochondria / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000145:classification / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D014157:Transcription Factors / Q000145:classification / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014908:Triticum / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure
chemical_list: D009687:Nuclear Proteins; D014157:Transcription Factors; C063552:transcription factor BTF3