title: Differential immuno-kinetic assays of allergen-specific binding for peanut allergy serum analysis.
abstract: A label-free nanoparticle array platform has been used to detect total peanut allergen-specific binding from whole serum of patients suffering from peanut allergy. The serum from 10 patients was screened against a four-allergen panel of cat and dog dander, dust mite and peanut allergen protein Ara h1. The IgE and IgG contributions to the total specific-binding protein load to Ara h1 were identified using two secondary IgG- and IgE-specific antibodies and were found to contribute less than 50 % of the total specific protein load. The total mass of IgE, IgE and the unresolved specific-binding protein ΔsBP for Ara h1 provides a new serum profile for high-RAST-grade patients 5 and 6 with the IgG/IgE ratio of 4 ± 2 and ΔsBP/IgE ratio of 17 ± 11, neither of which is protective for the small patient cohort.
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Exeter, UK.
authors.forename: Rouslan V
authors.initials: RV
authors.lastname: Olkhov
authors.forename: Edward R
authors.initials: ER
authors.lastname: Kaminski
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Shaw
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000378:metabolism*; D000818:Animals; D002415:Cats; D004285:Dogs; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007700:Kinetics; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry; D021183:Peanut Hypersensitivity / Q000097:blood* / Q000276:immunology; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: D000485:Allergens; D006046:Gold