title: [Expression of PRUNE2 mRNA and its positive correlation with non-coding RNA PCA3 in leiomyosarcoma].
abstract: OBJECTIVE
  Our previous study shows that PURNE2 mRNA plays an important role in the differential diagnosis of leiomyosarcoma and gastrointestinal stromal tumor (GIST). Non-coding RNA PCA3 locates in the intron of PRUNE2 and may play a role in PRUNE2 expression. The aim of this study was to explore the expression of PCA3 mRNA and PRUNE2 in leiomyosarcoma and their correlation.
  
  
  METHODS
  The expression of PRUNE2 mRNA was analyzed by agilent gene expression microarray CHIP in 31 leiomyosarcomas and 37 GISTs, and the correlation of the PRUNE2 expression and prognosis of leiomyosarcoma was predicted. Real-Time PCR assay was used to detect the mRNA levels of PCA3 and PRUNE2 in 13 leiomyosarcomas and to investigate their correlation. Seven prostate cancer tissues were used as control of PCA3.
  
  
  RESULTS
  The level of PRUNE2 mRNA expression was significantly higher in the 31 leiomyosarcomas than that in the 37 GISTs, and the level of PRUNE2 mRNA expression was correlated with survival of the leiomyosarcoma patients. Compared with prostate cancer, the non-coding RNA PCA3 expression level was significantly lower in leiomyosarcoma, and it had no correlation with the prognosis of leiomyosarcoma. Most importantly, the PRUNE2 and PCA3 mRNA expressions were both upregulated in leiomyosarcoma and showed a significant positive correlation.
  
  
  CONCLUSIONS
  Our findings demonstrate for the first time that PRUNE2 expression is correlated with the survival of leiomyosarcoma patients. Furthermore, non-coding RNA PCA3, which locates in the intron of PRUNE2, has a significant positive correlation with PRUNE2 and may play an important role in the pathogenesis of leiomyosarcoma.
authors.affiliation: Department of Bone and Soft Tissue Tumors, Tianjin Medical University Cancer Hospital, China. jilongyang2003@yahoo.com.cn
authors.forename: Ji-long
authors.initials: JL
authors.lastname: Yang
authors.forename: David
authors.initials: D
authors.lastname: Cogdell
authors.forename: James
authors.initials: J
authors.lastname: Eddy
authors.forename: Jonathan
authors.initials: J
authors.lastname: Trent
authors.forename: Nathan
authors.initials: N
authors.lastname: Price
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics / Q000378:metabolism; D046152:Gastrointestinal Stromal Tumors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007890:Leiomyosarcoma / Q000235:genetics / Q000378:metabolism*; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D022661:RNA, Untranslated / Q000378:metabolism; D012186:Retroperitoneal Neoplasms / Q000235:genetics / Q000378:metabolism*; D015996:Survival Rate; D014594:Uterine Neoplasms / Q000235:genetics / Q000378:metabolism
chemical_list: D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; C509845:PRUNE2 protein, human; D012333:RNA, Messenger; D022661:RNA, Untranslated; C479341:prostate cancer antigen 3, human