title: Development of protein-cage-based delivery nanoplatforms by polyvalently displaying β-cyclodextrins on the surface of ferritins through copper(I)-catalyzed azide/alkyne cycloaddition.
abstract: Protein cages are spherical hollow macromolecules that are attractive platforms for the construction of nanoscale cargo delivery vehicles. Human heavy-chain ferritin (HHFn) is modified genetically to control the number and position of functional groups per cage. 24 β-CDs are conjugated precisely to the modified HHFn in specific locations through thiol-maleimide Michael-type addition followed by copper(I)-catalyzed azide/alkyne cycloaddition (CuAAC). The resulting human ferritins displaying β-CDs (β-CD-C90 HHFn) can form inclusion complexes with FITC-AD, which can slowly release the guest molecule reversibly in a buffer solution via non-covalent β-CD/AD interactions. β-CD-C90 HHFn can potentially be used as delivery vehicles for insoluble drugs.
authors.affiliation: Bio-Molecular Informatics Center, Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea.
authors.forename: Chanho
authors.initials: C
authors.lastname: Kwon
authors.forename: Young Ji
authors.initials: YJ
authors.lastname: Kang
authors.forename: Sangbin
authors.initials: S
authors.lastname: Jeon
authors.forename: Seunho
authors.initials: S
authors.lastname: Jung
authors.forename: Sung You
authors.initials: SY
authors.lastname: Hong
authors.forename: Sebyung
authors.initials: S
authors.lastname: Kang
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001052:Apoferritins / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D002384:Catalysis; D003300:Copper / Q000737:chemistry*; D003432:Cross-Linking Reagents; D061565:Cycloaddition Reaction; D004337:Drug Carriers / Q000138:chemical synthesis*; D006801:Humans; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000480:Alkynes; D001386:Azides; D003432:Cross-Linking Reagents; D004337:Drug Carriers; D047392:beta-Cyclodextrins; D003300:Copper; D001052:Apoferritins