title: Effects of the propeptide of group X secreted phospholipase A(2) on substrate specificity and interfacial activity on phospholipid monolayers.
abstract: Group X secreted phospholipase A(2) (GX sPLA(2)) plays important physiological roles in the gastrointestinal tract, in immune and sperm cells and is involved in several types of inflammatory diseases. It is secreted either as a mature enzyme or as a mixture of proenzyme (with a basic 11 amino acid propeptide) and mature enzyme. The role of the propeptide in the repression of sPLA(2) activity has been studied extensively using liposomes and micelles as model interfaces. These substrates are however not always suitable for detecting some fine tuning of lipolytic enzymes. In the present study, the monolayer technique is used to compare PLA(2) activity of recombinant mouse GX sPLA(2) (mGX) and its pro-form (PromGX) on monomolecular films of dilauroyl-phosphatidyl-ethanolamine (DLPE), -choline (DLPC) and -glycerol (DLPG). The PLA(2) activity and substrate specificity of mGX (PE ≈ PG > PC) were found to be surface pressure-dependent. mGX displayed a high activity on DLPE and DLPG but not on DLPC monolayers up to surface pressures corresponding to the lateral pressure of biological membranes (30-35 mN/m). Overall, the propeptide impaired the enzyme activity, particularly on DLPE whatever the surface pressure. However some conditions could be found where the propeptide had little effects on the repression of PLA(2) activity. In particular, both PromGX and mGX had similar activities on DLPG at a surface pressure of 30 mN/m. These findings show that PromGX can be potentially active depending on the presentation of the substrate (i.e., lipid packing) and one cannot exclude such an activity in a physiological context. A structural model of PromGX was built to investigate how the propeptide controls the activity of GX sPLA(2). This model shows that the propeptide is located within the interfacial binding site (i-face) and could disrupt both the interfacial binding of the enzyme and the access to the active site by steric hindrance.
authors.affiliation: CNRS, Aix-Marseille Université, Enzymologie Interfaciale et Physiologie de la Lipolyse, UMR 7282, Marseille Cedex, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Point
authors.forename: Anaïs
authors.initials: A
authors.lastname: Bénarouche
authors.forename: Ikram
authors.initials: I
authors.lastname: Jemel
authors.forename: Goetz
authors.initials: G
authors.lastname: Parsiegla
authors.forename: Gérard
authors.initials: G
authors.lastname: Lambeau
authors.forename: Frédéric
authors.initials: F
authors.lastname: Carrière
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Cavalier
mesh_terms: D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004792:Enzyme Precursors / Q000737:chemistry / Q000378:metabolism; D054509:Group X Phospholipases A2 / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D008081:Liposomes / Q000737:chemistry; D008297:Male; D051379:Mice; D008823:Micelles; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D010712:Phosphatidic Acids / Q000737:chemistry / Q000378:metabolism; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D013499:Surface Properties
chemical_list: D004792:Enzyme Precursors; D008081:Liposomes; D008823:Micelles; D010455:Peptides; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C475742:dilauroylphosphatidic acid; C483858:phosphatidylethanolamine; C002448:1,2-linoleoylphosphatidylcholine; D054509:Group X Phospholipases A2