title: Energy-converting respiratory Complex I: on the way to the molecular mechanism of the proton pump.
abstract: In respiring organisms the major energy transduction flux employs the transmembrane electrochemical proton gradient as a physical link between exergonic redox reactions and endergonic ADP phosphorylation. Establishing the gradient involves electrogenic, transmembrane H(+) translocation by the membrane-embedded respiratory complexes. Among others, Complex I (NADH:ubiquinone oxidoreductase) is the most structurally complex and functionally enigmatic respiratory enzyme; its molecular mechanism is as yet unknown. Here we highlight recent progress and discuss the catalytic events during Complex I turnover in relation to their role in energy conversion and to the enzyme structure.
authors.affiliation: Helsinki Bioenergetics Group, Institute of Biotechnology, PO Box 65 (Viikinkaari 1) FIN-00014 University of Helsinki, Finland. marina.verkhovskaya@helsinki.fi
authors.forename: Marina
authors.initials: M
authors.lastname: Verkhovskaya
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Bloch
mesh_terms: D000818:Animals; D001419:Bacteria / Q000201:enzymology; D001665:Binding Sites; D004579:Electron Transport; D042967:Electron Transport Complex I / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007506:Iron-Sulfur Proteins / Q000737:chemistry / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D008958:Models, Molecular; D010084:Oxidation-Reduction; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D014451:Ubiquinone / Q000737:chemistry / Q000378:metabolism
chemical_list: D007506:Iron-Sulfur Proteins; D014451:Ubiquinone; D042967:Electron Transport Complex I