title: Development and degeneration of cone bipolar cells are independent of cone photoreceptors in a mouse model of retinitis pigmentosa.
abstract: Retinal photoreceptors die during retinal synaptogenesis in a portion of retinal degeneration. Whether cone bipolar cells establish regular retinal mosaics and mature morphologies, and resist degeneration are not completely understood. To explore these issues, we backcrossed a transgenic mouse expressing enhanced green fluorescent protein (EGFP) in one subset of cone bipolar cells (type 7) into rd1 mice, a classic mouse model of retinal degeneration, to examine the development and survival of cone bipolar cells in a background of retinal degeneration. Our data revealed that both the development and degeneration of cone bipolar cells are independent of the normal activity of cone photoreceptors. We found that type 7 cone bipolar cells achieved a uniform tiling of the retinal surface and developed normal dendritic and axonal arbors without the influence of cone photoreceptor innervation. On the other hand, degeneration of type 7 cone bipolar cells, contrary to our belief of central-to-peripheral progression, was spatially uniform across the retina independent of the spatiotemporal pattern of cone degeneration. The results have important implications for the design of more effective therapies to restore vision in retinal degeneration.
authors.affiliation: Department of Anatomy, The University of Hong Kong, Li Ka Shing Faculty of Medicine, Pokfulam, Hong Kong, People's Republic of China.
authors.forename: Miao
authors.initials: M
authors.lastname: Chen
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Lin
mesh_terms: D000375:Aging / Q000473:pathology; D000818:Animals; D001369:Axons / Q000378:metabolism; D048430:Cell Shape; D003712:Dendrites / Q000378:metabolism; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009030:Mosaicism; D051245:Retinal Bipolar Cells / Q000378:metabolism / Q000473:pathology*; D017949:Retinal Cone Photoreceptor Cells / Q000378:metabolism / Q000473:pathology*; D012174:Retinitis Pigmentosa / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
chemical_list: D049452:Green Fluorescent Proteins