title: Comparative study of the osteogenic differentiation capacity of human             bone marrow- and human adipose-derived stem cells under cyclic tensile stretch             using quantitative analysis.
abstract: Studies comparing the osteogenic differentiation capacity of human bone             marrow-derived stem cells (hBMSCs) and human adipose-derived stem cells (hASCs)             cultured in osteogenic differentiation medium have been inconclusive. Apart from             chemical stimuli, mechanical stimuli have also been shown to be important in bone             tissue engineering, which is referred to as functional bone tissue engineering.             hBMSCs and hASCs have been shown to be sensitive to both chemical and mechanical             stimuli. In an attempt to find a better seed cell in functional bone tissue engineering,             we tried to quantify the osteogenic differentiation capacity of hBMSCs and hASCs             under both mechanical and chemical stimuli. In this study, hBMSCs and hASCs were             isolated from the same volunteers. Cells were cultured in osteogenic differentiation             medium with and without exposure to cyclic tensile stretch (CTS). Quantitative             measurement of alkaline phosphatase (ALP) activity revealed that the osteogenic             differentiation capacity of hBMSCs was similar to that of hASCs in the early phase             of differentiation in the CTS-stimulated groups. Quantitative measurement of mineralization             showed that the late-phase osteogenic differentiation capacity of the hBMSCs was             superior to that of hASCs in the CTS-stimulated groups. Reverse transcription-polymerase             chain reaction (RT-PCR) analysis was performed 5 and 10 days after cell culture.             The results of the RT-PCR revealed that the osteogenic differentiation capacity             of hASCs was inferior to that of hBMSCs both in the CTS-stimulated and unstimulated             groups. All the results showed that both hBMSCs and hASCs were sensitive to CTS             during the osteogenic differentiation process. This study compared the osteogenic             differentiation capacity of hBMSCs and hASCs in response to mechanical stimulations             and has important implications for the use of stem cells in functional bone tissue             engineering and regenerative medicine.
authors.affiliation: Department of Orthopedics, Tongji Hospital, Tongji Medical College,                     Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Ye
authors.forename: Yu
authors.initials: Y
authors.lastname: Du
authors.forename: Fengjing
authors.initials: F
authors.lastname: Guo
authors.forename: Chen
authors.initials: C
authors.lastname: Gong
authors.forename: Kaixiang
authors.initials: K
authors.lastname: Yang
authors.forename: Liang
authors.initials: L
authors.lastname: Qin
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism; D001854:Bone Marrow Cells / Q000201:enzymology / Q000502:physiology*; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D048430:Cell Shape; D002470:Cell Survival; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D040542:Mechanotransduction, Cellular; D010012:Osteogenesis; D013234:Stem Cells / Q000201:enzymology / Q000502:physiology*; D050151:Subcutaneous Fat / Q000166:cytology*; D059467:Transcriptome; D055815:Young Adult
chemical_list: D000943:Antigens, Differentiation; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; C493709:RUNX2 protein, human; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; D002118:Calcium