title: Transcriptional regulation of human novel gene SPATA12 promoter by AP-1 and HSF.
abstract: Human SPATA12 is a spermatogenesis associated gene and is supposed to function as an inhibitor during male germ cell development. SPATA12 is specifically expressed in spermatocytes, spermatids, and spermatozoa of human testis. In order to understand the regulation mechanism of SPATA12 gene expression, we identified and characterized the SPATA12 gene core promoter region and transcription factor binding sites by using reporter gene assays. AP-1 is founded to be a potential transcriptional activator of SPATA12. The promoter activity of SPATA12 was drastically declined after AP-1 binding site mutation or deletion. We also demonstrated that AP-1 combined with Smad3/4 contributes to the transcriptional regulation of SPATA12 in response to TGF-β1. The expression of SPATA12 could be induced by TGF-β1 in a dose-dependent manner, suggesting that AP-1 as an activator plays a role in the regulation of SPATA12 promoter. We have also shown that heat shock treatment could activate the expression of SPATA12 and transcription factor HSF binding sites in the SPATA12 promoter might be responsible for this heat-induction. These results suggested that AP-1 and HSF may play an important role in regulating SPATA12 promoter activity.
authors.affiliation: Department of Life Science, School of Biology, Hunan University, Changsha 410082, China. lidanie@hotmail.com
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Yiting
authors.initials: Y
authors.lastname: Lin
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Liu
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhuoxian
authors.initials: Z
authors.lastname: Rong
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006367:HeLa Cells; D000076249:Heat Shock Transcription Factors; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D061986:MCF-7 Cells; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D013091:Spermatogenesis / Q000235:genetics / Q000502:physiology; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D018398:Homeodomain Proteins; C523865:SPATA12 protein, human; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D053773:Transforming Growth Factor beta1