title: Application of CGE to virus identification.
abstract: Protein profiling is an increasingly valuable tool for the characterization of protein populations and has been used to identify microorganisms, most often using two-dimensional gel electrophoresis followed by mass spectrometry. We present a rapid method for the identification of viruses using microfluidic chip gel electrophoresis (CGE) of high-copy number proteins to generate unique protein profiles. Viral proteins are solubilized, fluorescently labeled and then analyzed using the μChemLab™ CGE system (∼10 min overall). A Bayesian classification approach is used to classify the reproducible and visually distinct protein profiles of MS2 bacteriophage, Epstein-Barr, Respiratory Syncytial, and Vaccinia viruses as well as discriminate between closely related T2 and T4 bacteriophage.
authors.affiliation: Sandia National Laboratories, Livermore, CA, USA. jfruet@sandia.gov
authors.forename: Julia A
authors.initials: JA
authors.lastname: Fruetel
authors.forename: Victoria A
authors.initials: VA
authors.lastname: VanderNoot
mesh_terms: D017122:Bacteriophage T4 / Q000302:isolation & purification; D046209:Electrophoresis, Microchip / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005450:Fluorescamine / Q000378:metabolism; D012995:Solubility; D013223:Statistics as Topic; D014780:Viruses / Q000302:isolation & purification*
chemical_list: D005450:Fluorescamine