title: Ultraviolet irradiation-dependent fluorescence enhancement of hemoglobin catalyzed by reactive oxygen species.
abstract: Ultraviolet (UV) light has a potent effect on biological organisms. Hemoglobin, an oxygen-transport protein, plays an irreplaceable role in sustaining life of all vertebrates. In this study we scrutinize the effects of ultraviolet irradiation (UVI) as well as visible irradiation on the fluorescence characteristics of bovine hemoglobin (BHb) in vitro. Data show that UVI results in fluorescence enhancement of BHb in a dose-dependent manner. Furthermore, UVI-induced fluorescence enhancement is significantly increased when BHb is pretreated with hydrogen peroxide (H(2)O(2)), a type of reactive oxygen species (ROS). Meanwhile, The water-soluble antioxidant vitamin C suppresses this UVI-induced fluorescence enhancement. In contrast, green light irradiation does not lead to fluorescence enhancement of BHb no matter whether H(2)O(2) is acting on the BHb solution or not. Taken together, these results indicate that catalysis of ROS and UVI-dependent irradiation play two key roles in the process of UVI-induced fluorescence enhancement of BHb.
authors.affiliation: The Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, TEDA Applied Physics School and School of Physics, Nankai University, Tianjin, China. plt@nankai.edu.cn
authors.forename: Leiting
authors.initials: L
authors.lastname: Pan
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Wang
authors.forename: Shuying
authors.initials: S
authors.lastname: Yang
authors.forename: Xian
authors.initials: X
authors.lastname: Wu
authors.forename: Imshik
authors.initials: I
authors.lastname: Lee
authors.forename: Xinzheng
authors.initials: X
authors.lastname: Zhang
authors.forename: Romano A
authors.initials: RA
authors.lastname: Rupp
authors.forename: Jingjun
authors.initials: J
authors.lastname: Xu
mesh_terms: D000042:Absorption; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D055162:Biocatalysis / Q000187:drug effects* / Q000528:radiation effects*; D002417:Cattle; D004307:Dose-Response Relationship, Radiation; D005453:Fluorescence; D006454:Hemoglobins / Q000378:metabolism* / Q000528:radiation effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020011:Protective Agents / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
chemical_list: D006454:Hemoglobins; D020011:Protective Agents; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid