title: Swim training of monosodium L-glutamate-obese mice improves the impaired insulin receptor tyrosine phosphorylation in pancreatic islets.
abstract: The goal of the present study was to investigate changes on glucose homoeostasis and of the insulin receptor (IR) and insulin receptor substrate-1 (IRS-1) signalling in pancreatic islets from MSG-obese mice submitted to or not submitted to swim training. Swim training of 90-day-old MSG mice was used to evaluate whether signalling pathways of the IR and IRS-1 in islets are involved with the insulin resistance and glucose intolerance observed in this obese animal model. The results showed that IR tyrosine phosphorylation (pIR) was reduced by 42 % in MSG-obese mice (MSG, 6.7 ± 0.2 arbitrary units (a.u.); control, 11.5 ± 0.4 a.u.); on the other hand, exercise training increased pIR by 76 % in MSG mice without affecting control mice (MSG, 11.8 ± 0.3; control, 12.8 ± 0.2 a.u.). Although the treatment with MSG increased IRS-1 tyrosine phosphorylation (pIRS-1) by 96 % (MSG, 17.02 ± 0.6; control, 8.7 ± 0.2 a.u.), exercise training also increased it in both groups (control, 13.6 ± 0.1; MSG, 22.2 ± 1.1 a.u.). Current research shows that the practice of swim training increases the tyrosine phosphorylation of IRS-1 which can modulate the effect caused by obesity in insulin receptors.
authors.affiliation: Laboratory of Secretion Cell Biology, Department of Cell Biology and Genetics, State University of Maringá/UEM, Block H67, Room 19, Avenue Colombo, 5790, Maringá, PR, 87020-900, Brazil.
authors.forename: Rosiane Aparecida
authors.initials: RA
authors.lastname: Miranda
authors.forename: Renato Chaves Souto
authors.initials: RC
authors.lastname: Branco
authors.forename: Clarice
authors.initials: C
authors.lastname: Gravena
authors.forename: Luiz Felipe
authors.initials: LF
authors.lastname: Barella
authors.forename: Claudinéia Conationi
authors.initials: CC
authors.lastname: da Silva Franco
authors.forename: Ana Eliza
authors.initials: AE
authors.lastname: Andreazzi
authors.forename: Júlio Cezar
authors.initials: JC
authors.lastname: de Oliveira
authors.forename: Maria Cecília
authors.initials: MC
authors.lastname: Picinato
authors.forename: Paulo Cezar
authors.initials: PC
authors.lastname: de Freitas Mathias
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D009765:Obesity / Q000139:chemically induced / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011972:Receptor, Insulin / Q000378:metabolism*; D012970:Sodium Glutamate; D013550:Swimming / Q000502:physiology*
chemical_list: D001786:Blood Glucose; D007328:Insulin; D011972:Receptor, Insulin; D005947:Glucose; D012970:Sodium Glutamate