title: Inhibition of Staphylococcus epidermidis biofilm by trimethylsilane plasma coating.
abstract: Biofilm formation on implantable medical devices is a major impediment to the treatment of nosocomial infections and promotes local progressive tissue destruction. Staphylococcus epidermidis infections are the leading cause of biofilm formation on indwelling devices. Bacteria in biofilms are highly resistant to antibiotic treatment, which in combination with the increasing prevalence of antibiotic resistance among human pathogens further complicates treatment of biofilm-related device infections. We have developed a novel plasma coating technology. Trimethylsilane (TMS) was used as a monomer to coat the surfaces of 316L stainless steel and grade 5 titanium alloy, which are widely used in implantable medical devices. The results of biofilm assays demonstrated that this TMS coating markedly decreased S. epidermidis biofilm formation by inhibiting the attachment of bacterial cells to the TMS-coated surfaces during the early phase of biofilm development. We also discovered that bacterial cells on the TMS-coated surfaces were more susceptible to antibiotic treatment than their counterparts in biofilms on uncoated surfaces. These findings suggested that TMS coating could result in a surface that is resistant to biofilm development and also in a bacterial community that is more sensitive to antibiotic therapy than typical biofilms.
authors.affiliation: Division of Cardiovascular Medicine, Department of Internal Medicine, University of Missouri, Columbia, Missouri, USA.
authors.forename: Yibao
authors.initials: Y
authors.lastname: Ma
authors.forename: Meng
authors.initials: M
authors.lastname: Chen
authors.forename: John E
authors.initials: JE
authors.lastname: Jones
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Ritts
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Yu
authors.forename: Hongmin
authors.initials: H
authors.lastname: Sun
mesh_terms: D000497:Alloys / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002939:Ciprofloxacin / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D003428:Cross Infection / Q000517:prevention & control*; D004352:Drug Resistance, Microbial; D006801:Humans; D018613:Microscopy, Confocal; D058626:Plasma Gases; D019736:Prostheses and Implants / Q000382:microbiology*; D012821:Silanes / Q000737:chemistry / Q000494:pharmacology*; D013193:Stainless Steel / Q000737:chemistry; D013203:Staphylococcal Infections / Q000517:prevention & control*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000254:growth & development; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D058626:Plasma Gases; D012821:Silanes; D013193:Stainless Steel; D002939:Ciprofloxacin; D014025:Titanium