title: Solution NMR studies of integral polytopic α-helical membrane proteins: the structure determination of the seven-helix transmembrane receptor sensory rhodopsin II, pSRII.
abstract: About 30% of the proteins encoded in the genome are expressed as membrane proteins but these represent <1% of all the structures solved today. In view of the physiological and pharmaceutical significance of membrane proteins it is clear that a better and more comprehensive understanding of their three-dimensional (3D) structures at atomic resolution is required. α-Helical integral membrane proteins are generally more difficult to work with than β-barrel-type proteins and this has particularly been true for the polytopic members such as the large family of seven-helical proteins. In this chapter we describe the practical aspects of the solution-state NMR spectroscopy structure determination of the seven-helical transmembrane (7-TM) protein receptor sensory rhodopsin pSRII from the haloalkaliphilic archaeon Natronomonas pharaonis reconstituted in detergent micelles. This is the first time that a three-dimensional structure of a 7-TM protein has been determined by NMR.
authors.affiliation: Department of Biochemistry, University of Cambridge, Cambridge, UK.
authors.forename: Antoine
authors.initials: A
authors.lastname: Gautier
authors.forename: Daniel
authors.initials: D
authors.lastname: Nietlispach
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002338:Carotenoids / Q000737:chemistry* / Q000302:isolation & purification; D003902:Detergents / Q000737:chemistry; D007532:Isoleucine / Q000737:chemistry; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008565:Membrane Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D015203:Reproducibility of Results; D012995:Solubility; D012996:Solutions
chemical_list: D019843:Archaeal Proteins; D003902:Detergents; D008565:Membrane Proteins; D012996:Solutions; C434450:phototaxis receptor sensory rhodopsin II, Natronobacterium pharaonis; D007532:Isoleucine; D002338:Carotenoids