title: A differing role of oxidative stress in the regulation of central and peripheral hemodynamics during exercise in heart failure.
abstract: This study sought to characterize the role of free radicals in regulating central and peripheral hemodynamics at rest and during exercise in patients with heart failure (HF). We examined cardiovascular responses to dynamic handgrip exercise (4, 8, and 12 kg at 1 Hz) following consumption of either a placebo or acute oral antioxidant cocktail (AOC) consisting of vitamin C, E, and α-lipoic acid in a balanced, crossover design. Central and peripheral hemodynamics, mean arterial pressure, cardiac index, systemic vascular resistance (SVR), brachial artery blood flow, and peripheral (arm) vascular resistance (PVR) were determined in 10 HF patients and 10 age-matched controls. Blood assays evaluated markers of oxidative stress and efficacy of the AOC. When compared with controls, patients with HF exhibited greater oxidative stress, measured by malondialdehyde (+36%), and evidence of endogenous antioxidant compensation, measured by greater superoxide dismutase activity (+83%). The AOC increased plasma ascorbate (+50%) in both the HF patients and controls, but significant systemic hemodynamic effects were only evident in the patients with HF, both at rest and throughout exercise. Specifically, the AOC reduced mean arterial pressure (-5%) and SVR (-12%) and increased cardiac index (+7%) at each workload. In contrast, peripherally, brachial artery blood flow and PVR (arm) were unchanged by the AOC. In conclusion, these data imply that SVR in patients with HF is, at least in part, mediated by oxidative stress. However, this finding does not appear to be the direct result of muscle-specific changes in PVR.
authors.affiliation: Geriatric Research, Education, and Clinical Center, George E. Whalen Veterans Affairs Medical Center, Salt Lake City, UT 84148, USA. melissa.witman@utah.edu
authors.forename: Melissa A H
authors.initials: MA
authors.lastname: Witman
authors.forename: John
authors.initials: J
authors.lastname: McDaniel
authors.forename: Anette S
authors.initials: AS
authors.lastname: Fjeldstad
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Ives
authors.forename: Jia
authors.initials: J
authors.lastname: Zhao
authors.forename: Jose N
authors.initials: JN
authors.lastname: Nativi
authors.forename: Josef
authors.initials: J
authors.lastname: Stehlik
authors.forename: D Walter
authors.initials: DW
authors.lastname: Wray
authors.forename: Russell S
authors.initials: RS
authors.lastname: Richardson
mesh_terms: D000284:Administration, Oral; D000975:Antioxidants / Q000008:administration & dosage; D062186:Arterial Pressure; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001783:Blood Flow Velocity; D001916:Brachial Artery / Q000000981:diagnostic imaging / Q000503:physiopathology; D018592:Cross-Over Studies; D015444:Exercise; D018737:Hand Strength; D006333:Heart Failure / Q000000981:diagnostic imaging / Q000378:metabolism / Q000503:physiopathology*; D006439:Hemodynamics / Q000187:drug effects; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009119:Muscle Contraction; D018482:Muscle, Skeletal / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D012039:Regional Blood Flow; D016037:Single-Blind Method; D013482:Superoxide Dismutase / Q000097:blood; D008063:Thioctic Acid / Q000008:administration & dosage; D013997:Time Factors; D018608:Ultrasonography, Doppler; D014589:Utah; D014655:Vascular Resistance; D014810:Vitamin E / Q000008:administration & dosage
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D014810:Vitamin E; D008315:Malondialdehyde; D008063:Thioctic Acid; D013482:Superoxide Dismutase; D001205:Ascorbic Acid