title: The invasion mode of GH(3) cells is conditioned by collagen subtype, and its efficiency depends on cell-cell adhesion.
abstract: The adaptation of GH(3) cells to different microenvironments is a consequence of a partial compromise with the tumor phenotype. A collagen type IV enriched microenvironment favors an invasive phenotype and increases the substrate adhesion capacity, whereas it decreases the phosphorylation of the regulatory myosin light chain and the aggregation capacity. In contrast, the higher internal tension and increased aggregation capacity induced by collagen type I/III are factors that reduce the invasion rate. Our results show, for the first time, the importance of collagen subtypes in determining the migratory strategy: collagen I/III favors mesenchymal-like motility, whereas collagen type IV induces an ameboid-type displacement. The reciprocal modulation of the myosin light chain kinase and the Rho-kinase determines the invasive capacity through changes in tissue cohesion, extracellular matrix affinity, regulatory myosin light chain phosphorylation and spatial distribution. The collagen subtype determines which of the mechano-transduction signaling pathways will regulate the tensional homeostasis and affect the invasion ability as well as the preferred migration strategy of the cells.
authors.affiliation: Department of Physiology, Biophysics and Neuroscience, CINVESTAV-IPN, Av. Politécnico Nacional 2508, 07360 Mexico City, Mexico. azorin@xanum.uam.mx
authors.forename: Erika
authors.initials: E
authors.lastname: Azorín
authors.forename: Carmen
authors.initials: C
authors.lastname: Solano-Agama
authors.forename: M Eugenia
authors.initials: ME
authors.lastname: Mendoza-Garrido
mesh_terms: D000205:Actomyosin / Q000378:metabolism; D000236:Adenoma / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002449:Cell Aggregation / Q000502:physiology; D045744:Cell Line, Tumor; D003094:Collagen / Q000145:classification / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism; D024141:Collagen Type IV / Q000378:metabolism; D020409:Molecular Motor Proteins / Q000378:metabolism; D018994:Myosin Light Chains / Q000378:metabolism; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology / Q000503:physiopathology*; D010911:Pituitary Neoplasms / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D015398:Signal Transduction; D059016:Tumor Microenvironment / Q000502:physiology*; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D024141:Collagen Type IV; D020409:Molecular Motor Proteins; D018994:Myosin Light Chains; D003094:Collagen; D000205:Actomyosin; D054460:rho-Associated Kinases; D009219:Myosin-Light-Chain Kinase