title: Single-cell gel electrophoresis (Comet) assay in nano-genotoxicology.
abstract: The Comet assay, or single-cell gel electrophoresis assay, is an easy and simple yet reliable method to evaluate DNA damage in cells. Under the alkaline conditions of this method, DNA strand breaks and alkaline-labile sites are detected. Here we describe the alkaline version of the Comet assay with applications in testing nanoparticles.
authors.affiliation: Mary Babb Cancer Center, School of Medicine, West Virginia University, Morgantown, WV, USA.
authors.forename: Maricica
authors.initials: M
authors.lastname: Pacurari
authors.forename: Vincent
authors.initials: V
authors.lastname: Castranova
mesh_terms: D000468:Alkalies; D000818:Animals; D020552:Comet Assay / Q000379:methods*; D004247:DNA / Q000378:metabolism; D009153:Mutagens / Q000633:toxicity*; D053758:Nanoparticles / Q000633:toxicity*; D051381:Rats; D014116:Toxicology / Q000379:methods*
chemical_list: D000468:Alkalies; D009153:Mutagens; D004247:DNA