title: Differential cytokine expression in T-cell subsets of chicken caecal tonsils co-cultured with three species of Lactobacillus.
abstract: Members of the intestinal microbiota play an important role in the development of T-cells. Little is known about responses of intestinal T-cell subsets of chickens to commensal bacteria. Therefore, we set out to characterise cytokine responses in T-cells after exposure to lactobacilli. Caecal tonsil mononuclear cells were isolated and co-cultured with Lactobacillus acidophilus, Lactobacillus reuteri and Lactobacillus salivarius for 12 hours. Subsequently the CD4+ and CD8+ cells were fractionated by flow cytometry and the expression of pro- and anti-inflammatory cytokines as well as Toll-like receptor 21 (TLR21) was determined. The results demonstrated that chicken CD4+ and CD8+ T-cells express TLR21 and that the various isolates of lactobacilli differentially induces the expression of interleukin 10, interferon-gamma and transforming growth factor beta. Our results demonstrate that different Lactobacillus species have the capacity to regulate intestinal T-cell responses and that these responses may be important to intestinal homeostasis.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, 419 Gordon Street, Building 89, Guelph, Ontario N1G 2W1, Canada.
authors.forename: J T
authors.initials: JT
authors.lastname: Brisbin
authors.forename: P
authors.initials: P
authors.lastname: Parvizi
authors.forename: S
authors.initials: S
authors.lastname: Sharif
mesh_terms: D000818:Animals; D002432:Cecum / Q000276:immunology* / Q000382:microbiology; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D018920:Coculture Techniques; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D005260:Female; D015870:Gene Expression; D007778:Lactobacillus / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000382:microbiology; D010581:Peyer's Patches / Q000276:immunology* / Q000382:microbiology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000382:microbiology
chemical_list: D016207:Cytokines