title: The expression profiling of the CsPYL, CsPP2C and CsSnRK2 gene families during fruit development and drought stress in cucumber.
abstract: The abscisic acid (ABA) signal transduction core components in plants include the ABA receptors (PYR/PYL/RCARs), the group A type 2C protein phosphatases (PP2Cs) and the subclass III SNF1-related protein kinases 2 (SnRK2s). In this study, via homology cloning, three CsPYLs, four CsPP2Cs and two CsSnRK2s partial cDNAs were obtained in cucumber (Cucumis sativus). In silico analysis results indicated that all CsPYL, CsPP2C, and CsSnRK2 genes obtained are homologous to Arabidopsis AtPYL, AtPP2C, and AtSnRK2 genes, respectively. Based on phylogenetic analysis, only CsPP2C2 and CsSnRK2.2 belonged to group A PP2C and subclass III SnRK2, respectively, and may involve in ABA signal transduction. During cucumber fruit development and ripening, CsPYL2 and CsPP2C2 were highly expressed and both reached their peak value at 27 DAF, at which stage the ABA content was also at its highest level, which indicated that the CsPYL2 and CsPP2C2 may involve in transducing ABA signal in fruit and regulating fruit development and ripening. Under drought stress condition in cucumber seedlings, CsPYL1, CsPYL2, CsPP2C2 and CsSnKR2.2 were sensitive and up-regulated in root, stem and leaf; meanwhile, CsPYL3 showed a low sensitivity and were down-regulated in root and stem.
authors.affiliation: College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Chaorui
authors.initials: C
authors.lastname: Duan
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Shengjie
authors.initials: S
authors.lastname: Dai
authors.forename: Liang
authors.initials: L
authors.lastname: Sun
authors.forename: Kai
authors.initials: K
authors.lastname: Ji
authors.forename: Yufei
authors.initials: Y
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Canlei
authors.initials: C
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Luo
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Leng
mesh_terms: D000040:Abscisic Acid / Q000032:analysis / Q000378:metabolism*; D018533:Biomass; D002241:Carbohydrates / Q000032:analysis; D002734:Chlorophyll / Q000378:metabolism; D018553:Cucumis sativus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015536:Down-Regulation; D055864:Droughts; D005638:Fruit / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D000071636:Protein Phosphatase 2C; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D015398:Signal Transduction; D013312:Stress, Physiological; D015854:Up-Regulation
chemical_list: D002241:Carbohydrates; D010940:Plant Proteins; D002734:Chlorophyll; D000040:Abscisic Acid; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C