title: One step synthesis of gold-loaded radial mesoporous silica nanospheres and supported lipid bilayer functionalization: towards bio-multifunctional sensors.
abstract: A simple synthetic route is developed to achieve gold functionalized radial mesoporous silica nanoparticles (Au-MsNP) synthesized by a one step procedure fully compatible with basic conditions required for the preparation of monodispersed nanospheres. In a second step, Au-MsNP particles have been coated with phospholipid bilayers in order to design an advanced biofunctional platform with the gold metallic nanoparticles previously grown into the pore channels and responsible for a plasmonic activity relevant for biosensing. The size of Au-MsNP is checked by dynamic light scattering while zeta potential measurements reflect their surface charge. The particle morphology is characterized by transmission and scanning electron microscopy and the Si/Au ratios are obtained from energy dispersive X-ray analysis. The textural properties of Au-MsNP, specific surface area and pore size, are determined from N(2)  adsorption. The supported bilayers are achieved from vesicles of different phospholipids incubated with Au-MsNP particles. The coating efficiency is investigated by zeta potential and cryo- transmission electron microscopy. The plasmonic activities of bare Au-MsNP particles and coated lipid bilayer Au-MsNP platform are evidenced for two model systems: direct adsorption of bovine serum albumin and molecular recognition events between avidin molecules and biotin receptors integrated in the supported lipid bilayer.
authors.affiliation: Institut Charles Gerhardt Montpellier, Equipe MACS, 8 rue de l'Ecole, Normale, Montpellier Cedex 5, France.
authors.forename: Remi
authors.initials: R
authors.lastname: Veneziano
authors.forename: Gaelle
authors.initials: G
authors.lastname: Derrien
authors.forename: Sisareuth
authors.initials: S
authors.lastname: Tan
authors.forename: Alain
authors.initials: A
authors.lastname: Brisson
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Devoisselle
authors.forename: Joel
authors.initials: J
authors.lastname: Chopineau
authors.forename: Clarence
authors.initials: C
authors.lastname: Charnay
mesh_terms: D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D020285:Cryoelectron Microscopy; D006046:Gold / Q000737:chemistry* / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D054118:Nanospheres / Q000737:chemistry*; D010316:Particle Size; D017978:Receptors, Growth Factor / Q000737:chemistry / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry / Q000378:metabolism; D013499:Surface Properties
chemical_list: D008051:Lipid Bilayers; D017978:Receptors, Growth Factor; C052136:biotin receptor; D012710:Serum Albumin, Bovine; D006046:Gold; D012822:Silicon Dioxide