title: Anti-gal antibodies in α1,3-galactosyltransferase gene-knockout pigs.
abstract: Serum anti-galactose-α1,3-galactose (Gal) IgM and IgG antibody levels were measured by ELISA in α1,3-galactosyltransferase gene-knockout (GTKO) pigs (78 estimations in 47 pigs). A low level of anti-Gal IgM was present soon after birth, and rose to a peak at 4-6 m, which was maintained thereafter even in the oldest pigs tested (at >2 yr). Anti-Gal IgG was also present at birth, peaked at 3 m, and after 6 m steadily decreased until almost undetectable at 20 m. No differences in this pattern were seen between pigs of different gender. Total IgM followed a similar pattern as anti-Gal IgM, but total IgG did not decrease after 6m. The data provide useful baseline data for future experimental studies in GTKO pigs, e.g., relating to the antibody response to WT pig allografts.
authors.affiliation: Thomas E. Starzl Transplantation Institute, University of Pittsburgh Medical Center, Pittsburgh, PA 15261, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Fang
authors.forename: Anneke
authors.initials: A
authors.lastname: Walters
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Hara
authors.forename: Cassandra
authors.initials: C
authors.lastname: Long
authors.forename: Peter
authors.initials: P
authors.lastname: Yeh
authors.forename: David
authors.initials: D
authors.lastname: Ayares
authors.forename: David K C
authors.initials: DK
authors.lastname: Cooper
authors.forename: John
authors.initials: J
authors.lastname: Bianchi
mesh_terms: D000367:Age Factors; D000818:Animals; D005260:Female; D005700:Galactosyltransferases / Q000172:deficiency* / Q000235:genetics* / Q000276:immunology; D055786:Gene Knockout Techniques; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007518:Isoantibodies / Q000097:blood; D008297:Male; D034421:Sus scrofa / Q000235:genetics* / Q000276:immunology*; D014184:Transplantation, Homologous; D014312:Trisaccharides / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies; D014312:Trisaccharides; C076569:alpha-galactosyl epitope; D005700:Galactosyltransferases; C530267:alpha-1,3-galactosyltransferase 1, porcine