title: Dietary resveratrol prevents the development of food allergy in mice.
abstract: BACKGROUND
  Resveratrol is a bioactive polyphenol enriched in red wine that exhibits many beneficial health effects via multiple mechanisms. However, it is unclear whether resveratrol is beneficial for the prevention of food allergy. This study investigated whether resveratrol inhibited the development of food allergy by using a mouse model of the disease.
  
  
  RESULTS
  Mice fed standard diet or standard diet plus resveratrol were sensitized by intragastric administration of ovalbumin (OVA) and mucosal adjuvant cholera toxin (CT). Several manifestations of food allergy were then compared between the mice. The effects of resveratrol on T cells or dendritic cells were also examined by using splenocytes from OVA-specific T cell-receptor (TCR) transgenic DO11.10 mice or mouse bone marrow-derived dendritic cells (BMDCs) in vitro. We found that mice fed resveratrol showed reduced OVA-specific serum IgE production, anaphylactic reaction, and OVA-induced IL-13 and IFN-ã production from the mesenteric lymph nodes (MLNs) and spleens in comparison to the control mice, following oral sensitization with OVA plus CT. In addition, resveratrol inhibited OVA plus CT-induced IL-4, IL-13, and IFN-ã production in splenocytes from DO11.10 mice associated with inhibition of GATA-3 and T-bet expression. Furthermore, resveratrol suppressed the OVA plus CT-induced CD25 expression and IL-2 production in DO11.10 mice-splenocytes in association with decreases in CD80 and CD86 expression levels. Finally, resveratrol suppressed CT-induced cAMP elevation in association with decreases in CD80 and CD86 expression levels in BMDCs.
  
  
  CONCLUSIONS
  Ingestion of resveratrol prevented the development of a food allergy model in mice. Given the in vitro findings, resveratrol might do so by inhibiting DC maturation and subsequent early T cell activation and differentiation via downregulation of CT-induced cAMP activation in mice. These results suggest that resveratrol may have potential for prophylaxis against food allergy.
authors.affiliation: Department of Immunology, University of Yamanashi Faculty of Medicine Chuo, Yamanashi, Japan.
authors.forename: Yui
authors.initials: Y
authors.lastname: Okada
authors.forename: Kyoko
authors.initials: K
authors.lastname: Oh-oka
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Kayoko
authors.initials: K
authors.lastname: Ishimaru
authors.forename: Shuji
authors.initials: S
authors.lastname: Matsuoka
authors.forename: Ko
authors.initials: K
authors.lastname: Okumura
authors.forename: Hideoki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Masashi
authors.initials: M
authors.lastname: Hisamoto
authors.forename: Tohru
authors.initials: T
authors.lastname: Okuda
authors.forename: Atsuhito
authors.initials: A
authors.lastname: Nakao
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D002772:Cholera Toxin / Q000008:administration & dosage; D000242:Cyclic AMP / Q000097:blood / Q000276:immunology; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D005260:Female; D005512:Food Hypersensitivity / Q000209:etiology / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D050990:GATA3 Transcription Factor / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D016898:Interferon-alpha / Q000097:blood / Q000276:immunology; D018793:Interleukin-13 / Q000097:blood / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000276:immunology; D008198:Lymph Nodes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010047:Ovalbumin / Q000009:adverse effects; D000077185:Resveratrol; D015398:Signal Transduction; D013154:Spleen / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D020825:T-Box Domain Proteins / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D000098815:T-bet Transcription Factor
chemical_list: D015703:Antigens, CD; D000975:Antioxidants; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D016898:Interferon-alpha; D018793:Interleukin-13; D013267:Stilbenes; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin; D002772:Cholera Toxin; D000242:Cyclic AMP; D000077185:Resveratrol