title: Natural deletion of L35Y36 in p6 gag eliminate LYPXnL/ALIX auxiliary virus release pathway in HIV-1 subtype C.
abstract: Natural loss of L35Y36 residues in ALIX binding site of HIV-1 subtype C was found to prevent the p6 gag-ALIX interaction. Over expression of ALIX 364-716 (V-domain) unlike pNL4.3 (subtype B), also did not inhibit the release of chimeric pNL4.3 expressing subtype C p6 late domain. Loss of V domain binding consequently affected the ALIX mediated particle release in the absence of PTAP/TSG101 pathway. Our data indicated absence of LYPXnL/ALIX pathway in HIV-1 subtype C.
authors.affiliation: Department of Molecular Virology, National AIDS Research Institute, G-73 MIDC, Bhosari, Pune, India.
authors.forename: Ajit
authors.initials: A
authors.lastname: Patil
authors.forename: Jayanta
authors.initials: J
authors.lastname: Bhattacharya
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D023061:Gene Order; D015497:HIV-1 / Q000145:classification / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017384:Sequence Deletion; D057074:Virus Release / Q000235:genetics; D014779:Virus Replication; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000737:chemistry* / Q000235:genetics*
chemical_list: D054301:gag Gene Products, Human Immunodeficiency Virus; C083002:p6 gag protein, Human immunodeficiency virus 1