title: Interrelationship and expression profiling of cyclooxygenase and angiogenic factors in Indian patients with multiple myeloma.
abstract: Multiple myeloma (MM) is classically illustrated by a desynchronized cytokine system with rise in inflammatory cytokines. There are recent reports which emphasized the potential role of angiogenesis in the development of MM. Role of cyclooxygenase 2 (COX-2) is well documented in the pathogenesis of solid tumors, but little is known about its occurrence and function in hematologic neoplasms. Involvement of neoangiogenesis is reported in the progression of MM, and angiopoietins probably contribute to this progression by enhancing neovascularization. Circulatory and mRNA levels of angiogenic factors and cyclooxygenase were determined in 125 subjects (75 MM patients and 50 healthy controls) by using enzyme-linked immunosorbent assay and quantitative PCR. We observed significant increase for angiogenic factors (Ang-1, Ang-2, hepatocyte growth factor, and vascular endothelial growth factor) and cyclooxygenase at circulatory level, as well as at mRNA level, as compared to healthy controls except insignificant increase for Ang-1 at circulatory level. We have also observed the significant positive correlation of all angiogenic factors with cyclooxygenase. The strong association found between angiogenic factors and COX-2 in this study may lead to the development of combination therapeutic strategy to treat MM. Therefore, targeting COX-2 by using its effective inhibitors demonstrating antiangiogenic and antitumor effects could be used as a new therapeutic approach for treatment of MM.
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, 110029, India.
authors.forename: Rehan
authors.initials: R
authors.lastname: Khan
authors.forename: Manoj
authors.initials: M
authors.lastname: Sharma
authors.forename: Lalit
authors.initials: L
authors.lastname: Kumar
authors.forename: Syed Akhtar
authors.initials: SA
authors.lastname: Husain
authors.forename: Alpana
authors.initials: A
authors.lastname: Sharma
mesh_terms: D000328:Adult; D000368:Aged; D020533:Angiogenesis Inhibitors / Q000627:therapeutic use; D042683:Angiopoietin-1 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D042702:Angiopoietin-2 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D020869:Gene Expression Profiling; D016298:Hematopoietic Cell Growth Factors / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood / Q000188:drug therapy / Q000235:genetics*; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D009389:Neovascularization, Pathologic / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000097:blood; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
chemical_list: C467630:ANGPT1 protein, human; D020533:Angiogenesis Inhibitors; D042683:Angiopoietin-1; D042702:Angiopoietin-2; D016298:Hematopoietic Cell Growth Factors; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human