title: In vitro safety toxicology data for evaluation of gold nanoparticles-chronic cytotoxicity, genotoxicity and uptake.
abstract: Safety and toxic effects of nanoparticles are still largely unexplored due to the multiple aspects that influence their behaviour toward biological systems. Here, we focus the attention on 12 nm spherical gold nanoparticle coated or not with hyaluronic acid compared to its precursor counterpart salt. Results ranging from the effects of a 10-days exposure in an in vitro model with BALB/c 3T3 fibroblast cells show how 12 nm spherical gold nanoparticles are internalized from 3T3 cells by endo-lysosomal pathway by an indirect measurement technique; and how gold nanoparticles, though not being a severe cytotoxicant, induce DNA damage probably through an indirect mechanism due to oxidative stress. While coating them with hyaluronic acid reduces gold nanoparticles cytotoxicity and slows their cell internalization. These results will be of great interest to medicine, since they indicate that gold nanoparticles (with or without coating) are suitable for therapeutic applications due to their tunable cell uptake and low toxicity.
authors.affiliation: Unit of Experimental Toxicology and Ecotoxicology, Parc Científic Barcelona (UTOX-PCB), Barcelona, Spain.
authors.forename: Claudia
authors.initials: C
authors.lastname: Di Guglielmo
authors.forename: Joaquín
authors.initials: J
authors.lastname: De Lapuente
authors.forename: Constança
authors.initials: C
authors.lastname: Porredon
authors.forename: David
authors.initials: D
authors.lastname: Ramos-López
authors.forename: Judith
authors.initials: J
authors.lastname: Sendra
authors.forename: Miquel
authors.initials: M
authors.lastname: Borràs
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015197:Carcinogenicity Tests; D004249:DNA Damage; D006046:Gold / Q000737:chemistry*; D066298:In Vitro Techniques; D053768:Metal Nanoparticles; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D009152:Mutagenicity Tests
chemical_list: D006046:Gold