title: Regulation of glucose transporter protein-1 and vascular endothelial             growth factor by hypoxia inducible factor 1α under hypoxic conditions in Hep-2             human cells.
abstract: The present study evaluated the regulation of glucose transporter protein-1 (Glut-1) and vascular endothelial growth factor (VEGF) by hypoxia inducible factor 1α (HIF-1α) under hypoxic conditions in Hep-2 human cells to explore the feasibility of these three genes as tumor markers. Hep-2 cells were cultured under hypoxic and normoxic conditions for 6, 12, 24, 36 and 48 h. The proliferation of Hep-2 cells was evaluated using an MTT assay. The protein and mRNA expression levels of HIF-1α, Glut-1 and VEGF were detected using the S-P immunocytochemical method, western blotting and reverse transcription polymerase chain reaction (RT-PCR). The results revealed that the expression levels of HIF-1α, Glut-1 and VEGF protein in Hep-2 cells were significantly elevated under hypoxic conditions compared with those under normoxic conditions over 36 h. Under hypoxic conditions, mRNA levels of HIF-1α were stable, while mRNA levels of Glut-1 and VEGF changed over time. In conclusion, Glut-1 and VEGF were upregulated by HIF-1α under hypoxic conditions in a time-dependent manner in Hep-2 cells and their co-expression serves as a tumor marker.
authors.affiliation: Department of Otorhinolaryngology, Hebei Medical University,                     Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Ou
authors.initials: O
authors.lastname: Xu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Qu
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: An
authors.forename: Maoxin
authors.initials: M
authors.lastname: Wang
authors.forename: Qingjia
authors.initials: Q
authors.lastname: Sun
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiuying
authors.initials: X
authors.lastname: Lu
authors.forename: Lihong
authors.initials: L
authors.lastname: Pi
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Shen
mesh_terms: D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D012333:RNA, Messenger / Q000378:metabolism; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism*
chemical_list: D051272:Glucose Transporter Type 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A