title: Evaluation of mutagenic and antimutagenic activities of oligorutin and oligoesculin.
abstract: Rutin and esculin have been polymerised by laccase. Five fractions with M(w)¯ between 2127.42 and 8331.85g/mol for oligorutins, and between 688.12 and 6973g/mol for oligoesculins, were obtained. Fourier transformed infrared analysis showed that oligorutins were formed through C-C, C-O and CO linkages, while oligoesculins were obtained through C-C linkages. Monomers, their oligomers and their metabolites exhibited no mutagenic effect. Oligorutins and oligoesculins were more efficient in reducing the mutagenicity of methyl methanesulphonate, by, respectively, 69% and 64.8% in the presence of Salmonella typhimurium TA104, and 79.7% and 68.9% in the presence of S. typhimurium TA102, than were their monomers. The same oligomers revealed greater significant inhibitory effect of 2-aminoanthracene mutagenicity (respectively 82.4% and 79.3% in the presence of S. typhimurium TA104, and 89.2% and 82.9% in the presence of S. typhimurium TA102), than their monomers. Our results strongly suggest the enhancement of the tested monomer antimutagenicity after polymerisation.
authors.affiliation: Laboratory of Biomolecular Engineering, National School of Agronomy and Food Industries, National Polytechnics Institute of Lorraine ENSAIA-INPL, 54505 Vandoeuvre les Nancy, France.
authors.forename: Ghada
authors.initials: G
authors.lastname: Ben Rhouma
authors.forename: Latifa
authors.initials: L
authors.lastname: Chebil
authors.forename: Mounira
authors.initials: M
authors.lastname: Krifa
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ghoul
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D004929:Esculin / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000494:pharmacology*; D058105:Polymerization; D051381:Rats; D012431:Rutin / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D016587:Antimutagenic Agents; D009153:Mutagens; D004929:Esculin; D012431:Rutin