title: Adoptive cell therapy with autologous tumor-infiltrating lymphocytes and high-dose interleukin-2 for metastatic melanoma: The surgeon's perspective.
abstract: Tumor-infiltrating lymphocytes (TILs) are produced by resecting tumor tissue and growing and expanding ex vivo large quantities of autologous T cells. Once the TILs are ready for infusion, the patient undergoes a non-myeloablative lympho-depleting course of chemotherapy and subsequent TIL infusion with high-dose bolus IL-2. This study reviews the surgical experience of the TIL program at the Chaim Sheba Cancer Research Center in Israel. Eligible patients underwent surgical consultation to determine what tumorectomy would be beneficial for harvesting appropriate tissue. Factors involved in the decision included tumor mass size, location and morbidity of the procedure. Between January 2006 and May 2010, 44 patients underwent 47 procedures of adoptive transfer of TILs. Three patients underwent the procedure twice for recurrence after initial good responses, including an additional surgical procedure to produce fresh tumor. Thirty-seven excisions were with general anesthesia and 10 were with local anesthesia. Of the 37 general anesthesia procedures, 27 were open procedures involving a thoracotomy, a laparotomy or dissection of a major lymph node basin. Ten used minimally invasive techniques such as thorascopy or laparoscopy. Tumorectomy sites included 18 lymph node metastasis, 13 subcutaneous nodules, 11 lung specimens and 5 abdominal visceral metastasis including 2 liver lesions. Surgical mortality and major morbidity was 0%. Minor morbidity included only wound complications. Maximal number of TILs were derived from lymph node specimens, while liver metastasis procured the fewest TILs. Adoptive cell transfer technology affords a maximal tumor response with minimal surgical morbidity in metastatic patients.
authors.affiliation: Departments of Surgical Oncology.
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Zippel
authors.forename: Michal
authors.initials: M
authors.lastname: Besser
authors.forename: Roni
authors.initials: R
authors.lastname: Shapira
authors.forename: Alon
authors.initials: A
authors.lastname: Ben-Nun
authors.forename: David
authors.initials: D
authors.lastname: Goitein
authors.forename: Tima
authors.initials: T
authors.lastname: Davidson
authors.forename: Abraham J
authors.initials: AJ
authors.lastname: Treves
authors.forename: Gal
authors.initials: G
authors.lastname: Markel
authors.forename: Jacob
authors.initials: J
authors.lastname: Schachter
authors.forename: Moshe Z
authors.initials: MZ
authors.lastname: Papa