title: Inhibition of essential bacterial peptidyl-tRNA hydrolase activity by tropical plant extracts.
abstract: Peptidyl-tRNA Hydrolase (Pth) is a highly conserved, essential enzyme in bacteria. It removes the peptide portion from peptidyl-tRNA, returning free tRNAs to participate in translation. Build-up of peptidyl-tRNAs is toxic and defects in Pth function result in cell death. Herein we use in vitro activity of recombinant E. coli Pth to screen tropical plant extracts for inhibition. Multiple extracts were found to have inhibitory activity with some exhibiting different inhibitory effects depending on extraction conditions. IC50 values ranged from 0.02 to > 53.8 microg of extract per 1 unit of Pth, holding promise for in vivo screening. The inhibitory components in these extracts may serve as lead compounds for development of novel antibacterials.
authors.affiliation: Department of Chemistry, University of Alabama in Huntsville, 301 Sparkman Drive, Huntsville, AL 35899, USA.
authors.forename: Hana
authors.initials: H
authors.lastname: McFeeters
authors.forename: Morgan J
authors.initials: MJ
authors.lastname: Gilbert
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Thompson
authors.forename: William N
authors.initials: WN
authors.lastname: Setzer
authors.forename: Luis R
authors.initials: LR
authors.lastname: Cruz-Vera
authors.forename: Robert L
authors.initials: RL
authors.lastname: McFeeters
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014329:Tropical Climate
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D002265:Carboxylic Ester Hydrolases; C019674:aminoacyl-tRNA hydrolase