title: Variations in the composition of Fructus Evodiae after processing with Radix Glycyrrhizae extract.
abstract: OBJECTIVE
  To evaluate the changes in the concentrations of five components in Fructus Evodiae used in Chinese medicine, including evodiamine and glycyrrhizic acid, during processing of Fructus Evodiae with Radix Glycyrrhizae extract by using high performance liquid chromatography (HPLC) and to provide a scientific basis for different clinical uses of processed and unprocessed Fructus Evodiae.
  
  
  METHODS
  The concentrations of the Fructus Evodiae components in processed or unprocessed Fructus Evodiae were evaluated by HPLC using a YMC J'sphere ODS-H80 column (4.6 mm×250 mm, 5 μm) with acetonitrile-water-tetrahydrofuran-acetic acid (41:59:1:0.2, v/v/v/v) as the mobile phase. The detection wavelength was 225 nm, the column temperature was 35°C, the flow rate was 1.0 mL/min, and the injection volume was 10 μL. The concentrations of the Radix Glycyrrhizae components were determined by HPLC with a Kromasil-C₁₈ column (4.6 mm×250 mm, 4 μm) and a gradient elution of acetonitrile (A) and 0.05% aqueous phosphoric acid (B) as the mobile phase. The detection wavelength was 237 nm, the column temperature was 35 °C, the flow rate was 1.0 mL/min, and the injection volume was 10 μL.
  
  
  RESULTS
  The calibration curves of evodia lactone, evodiamine, rutaecarpine, liquiritin, and glycyrrhizin showed good linear relationships (r>0.99). The recoveries of evodia lactone, evodiamine, rutaecarpine, liquiritin, and glycyrrhizin were 96.59%, 104.18%, 101.91%, 97.75%, and 97.95%, respectively. The concentrations of the components in processed Fructus Evodiae were obviously different to those in unprocessed Fructus Evodiae.
  
  
  CONCLUSIONS
  The developed method is rapid and accurate. The results provide a reference for processed Fructus Evodiae and the changes that could be expected in its effects compared to unprocessed Fructus Evodiae.
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
authors.forename: Bi-ying
authors.initials: BY
authors.lastname: Xiao
authors.forename: Shu-jie
authors.initials: SJ
authors.lastname: Mao
authors.forename: Xian-duan
authors.initials: XD
authors.lastname: Li
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003296:Cooking; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D032086:Evodia / Q000737:chemistry*; D029882:Glycyrrhiza uralensis; D010936:Plant Extracts / Q000032:analysis*; D012015:Reference Standards; D012996:Solutions
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012996:Solutions