title: Outcome of allogeneic haematopoietic stem cell transplantation in aplastic anaemia.
abstract: OBJECTIVE
  To analyze factors associated with survival, rejection and graft versus host disease in aplastic anaemia patients undergoing allogeneic haematopoietic stem cell transplantation (SCT) from HLA matched sibling donors.
  
  
  METHODS
  Analytical study.
  
  
  METHODS
  Armed Forces Bone Marrow Transplant Centre, Rawalpindi, Pakistan from July 2001 to June 2010.
  
  
  METHODS
  Consecutive aplastic anaemia (AA) patients undergoing haematopoietic stem cell transplantation from HLA-matched sibling donors at this centre were included in this study. Potential factors affecting overall survival, rejection, disease-free survival and graft versus host disease were analyzed. Survival analysis was done by Kaplan-Meier method. Cox regression model was applied for multivariate analysis.
  
  
  RESULTS
  Ninety male and thirty-five female patients with AA were included in the study. Median age was 18 years. Conditioning regimens used were cyclophosphamide (Cy) plus antilymphocyte globulin (ALG) or antithymocyte globulin (ATG), fludarabine (FLU) +Cy+ATG, Campath 1-H +Cy in 89, 30 and 6 cases respectively. GVHD prophylaxis used was ciclosporin (CSA) plus prednisolone and short methotrexate in 81 while 44 received CSA plus prednisolone. At a median follow-up of 1185 days OS and DFS were 84% and 78% respectively. Factors associated with better OS were male sex, Flu/Cy/ATG conditioning and use of bone marrow as stem cell source.
  
  
  CONCLUSIONS
  Flu/Cy/ATG conditioning regimen, bone marrow as stem cell source and CSA, prednisolone and short methotrexate regimen were associated with better survival in AA.
authors.affiliation: Department of Haematology, Armed Forces Bone Marrow Transplant Centre, Rawalpindi. parvez101@yahoo.com
authors.forename: Parvez
authors.initials: P
authors.lastname: Ahmed
authors.forename: Qamar-un-Nisa
authors.initials: QU
authors.lastname: Chaudhry
authors.forename: Shahid
authors.initials: S
authors.lastname: Raza
authors.forename: Syed Kamran
authors.initials: SK
authors.lastname: Mahmood
authors.forename: Tariq Mehmood
authors.initials: TM
authors.lastname: Satti
authors.forename: Masood
authors.initials: M
authors.lastname: Anwar
authors.forename: Chaudhry
authors.initials: C
authors.lastname: Altaf
authors.forename: Nighat
authors.initials: N
authors.lastname: Shahbaz
authors.forename: Humayoon Shafique
authors.initials: HS
authors.lastname: Satti
mesh_terms: D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000401:mortality / Q000473:pathology / Q000628:therapy*; D001706:Biopsy; D005260:Female; D005500:Follow-Up Studies; D006086:Graft vs Host Disease; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006789:Host vs Graft Reaction; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D010154:Pakistan; D012307:Risk Factors; D016019:Survival Analysis; D019172:Transplantation Conditioning / Q000379:methods*; D014184:Transplantation, Homologous; D016896:Treatment Outcome
chemical_list: D007166:Immunosuppressive Agents