title: Chitinophaga cymbidii sp. nov., isolated from Cymbidium goeringii roots.
abstract: Two Gram-staining-negative, aerobic, non-spore-forming rod-shaped, non-motile bacteria, designated strains R156-2(T) and T58-2 were isolated from the roots of Cymbidium goeringii. The colonies were yellow-pigmented. On the basis of 16S rRNA gene sequence similarity, strains R156-2(T) and T58-2 were shown to be members of the genus Chitinophaga. Strains R156-2(T) and T58-2 showed the greatest level of sequence similarity with Chitinophaga niabensis (96.0-96.3 %). The major menaquinone was MK-7. The main cellular fatty acids were iso-C15 : 0, C16 : 1ω5c and iso-C17 : 0 3-OH. Phenotypic and genotypic analyses indicated that strains R156-2(T) and T58-2 could not be assigned to any recognized species. Therefore, strains R156-2(T) and T58-2 represent a novel species of the genus Chitinophaga, for which the name Chitinophaga cymbidii sp. nov. is proposed. The type strain is R156-2(T) ( = ACCC 05363(T) = KCTC 23738(T)). The DNA G+C content of this strain is 51.9 mol%.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, PR China.
authors.forename: Lubin
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Life Sciences, Hebei University, Baoding 071002, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Hebei University, Baoding 071002, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Shi
authors.affiliation: College of Life Sciences, Hebei University, Baoding 071002, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Guo
authors.affiliation: College of Life Sciences, Hebei University, Baoding 071002, PR China.
authors.forename: Aifang
authors.initials: A
authors.lastname: Xu
authors.affiliation: Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, PR China.
authors.forename: Na
authors.initials: N
authors.lastname: Yao
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7