title: Expression of the molecular chaperone αB-crystallin in infiltrating ductal breast carcinomas and the significance thereof: an immunohistochemical and proteomics-based strategy.
abstract: This study aims to evaluate αB-crystallin expression in infiltrating ductal breast carcinomas (IDCAs), as well as, its prognostic significance. Using a two-dimensional electrophoresis matrix-assisted laser desorption/ionisation-time of flight mass spectrometry investigation coupled to an immunohistochemical approach, we have assessed the expression of αB-crystallin in IDCAs, as well as, in other types of breast tumors (invasive lobular carcinomas, medullary carcinomas, and in situ ductal carcinomas). Correlation between αB-crystallin expression and clinicopathological parameters of breast cancer has also been investigated. Proteomic analyses revealed an increased expression of αB-crystallin in IDCA tumors compared to adjacent nontumor tissues. Overexpression of this molecular chaperone was further confirmed in 51 tumor specimens. Statistical analyses revealed, however, no significant correlations between αB-crystallin expression and clinicopathological parameters of the disease (tumor stage, patient age, hormone receptors, SBR grade, and lymph node metastases). This study demonstrates the upregulation of αB-crystallin in IDCA tissues which may highlight its possible involvement in breast cancer development. Our findings do not, however, support the involvement of this molecular chaperone in the progression of this disease.
authors.affiliation: Laboratoire d'Immuno-Oncologie Moléculaire, Faculté de Médecine de Monastir, Monastir, Tunisia.
authors.forename: Maria
authors.initials: M
authors.lastname: Kabbage
authors.forename: Mounir
authors.initials: M
authors.lastname: Trimeche
authors.forename: Hela
authors.initials: H
authors.lastname: Ben Nasr
authors.forename: Philippe
authors.initials: P
authors.lastname: Hammann
authors.forename: Lauriane
authors.initials: L
authors.lastname: Kuhn
authors.forename: Bechr
authors.initials: B
authors.lastname: Hamrita
authors.forename: Anouar
authors.initials: A
authors.lastname: Chaieb
authors.forename: Lotfi
authors.initials: L
authors.lastname: Chouchane
authors.forename: Karim
authors.initials: K
authors.lastname: Chahed
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001940:Breast / Q000378:metabolism* / Q000473:pathology; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008207:Lymphatic Metastasis; D008875:Middle Aged; D060787:Neoplasm Grading; D011379:Prognosis; D040901:Proteomics; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D038203:alpha-Crystallin B Chain / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C059215:CRYAB protein, human; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D038203:alpha-Crystallin B Chain