title: TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer.
abstract: TRIM28 is a universal corepressor for Kruppel-associated box zinc finger proteins. In this study, we demonstrated the expression of TRIM28 gene was significantly higher in cancerous tissues than in noncancerous tissues (P < 0.001). TRIM28 knockdown resulted in a decrease in cell proliferation in liquid media as well as in soft agar. The proliferation rate was impaired and the cell cycle progression was inhibited after knockdown of TRIM28 in non-small cell lung cancer cell lines PAa and SK-MES-1. We used real-time polymerase chain reaction to detect circulating cancer cells in 138 non-small cell lung cancer patients. The overall positive detection rate was 30.4% (42 of 138) in peripheral blood of NSCLC patients and was 29.9% (29 of 97) in early-stage patients. In a 70-month follow-up study, 20 of 29 patients (69.0%) in TRIM28 positive group had recurrence and/or metastasis, significantly higher (P = 0.004) than in the TRIM28 negative group (25 of 68, 36.8%). In addition, non-small cell lung cancer patients whose circulating cancer cells expressed TRIM28 suffered shorter tumor-specific survival compared with those with absent TRIM28 expression (P < 0.001). Results of our study showed that TRIM28 provides a survival advantage to lung cancer cells and may be a new marker to predict metastasis and prognosis in early-stage non-small cell lung cancer patients.
authors.affiliation: Department of Immunology, Basic Medical Institute, Chengde Medical College, Chengde, Hebei Province, 067000, China. homingreceptor@hotmail.com
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Enhong
authors.initials: E
authors.lastname: Zhao
authors.forename: Chunhui
authors.initials: C
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Huang
authors.forename: Lijun
authors.initials: L
authors.lastname: Xiao
authors.forename: Luyang
authors.initials: L
authors.lastname: Cheng
authors.forename: Xu
authors.initials: X
authors.lastname: Huang
authors.forename: Youxin
authors.initials: Y
authors.lastname: Song
authors.forename: Dawei
authors.initials: D
authors.lastname: Xu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000096:biosynthesis*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D002453:Cell Cycle / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D018450:Disease Progression; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009360:Neoplastic Cells, Circulating / Q000473:pathology; D012097:Repressor Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D016019:Survival Analysis; D014162:Transfection; D000076183:Tripartite Motif-Containing Protein 28; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D012097:Repressor Proteins; C101116:TRIM28 protein, human; D000076183:Tripartite Motif-Containing Protein 28