title: In vivo methods of nanotoxicology.
abstract: The new field of nanotoxicology is steadily emerging in parallel with rapid advances made in nanotechnology to evaluate biological impact of intended and non-intended nanomaterial exposure over time as their human applications constantly increase. Over the last decade nanotoxicology methods have mostly relied on in vitro cell-based characterizations that do not account for the complexity of in vivo systems with respect to biodistribution, metabolism, hematology, immunology, and neurological ramifications. Comprehensive in vivo studies addressing the toxicity of nanoscale materials are scarce mainly because the field is still nascent. Efforts in standardizing methodology to study the in vivo safety of these materials are currently undertaken by various government agencies and research organizations. Here, we discuss the need for in vivo nanotoxicity studies, outline some of the important methods, and comment on practical considerations in carrying out such studies.
authors.affiliation: Department of Pharmacology & Toxicology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Khaled
authors.initials: K
authors.lastname: Greish
authors.forename: Giridhar
authors.initials: G
authors.lastname: Thiagarajan
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Ghandehari
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D006801:Humans; D009153:Mutagens / Q000633:toxicity; D049329:Nanostructures / Q000633:toxicity*; D036103:Nanotechnology / Q000379:methods*; D014018:Tissue Distribution / Q000187:drug effects; D018675:Toxicity Tests
chemical_list: D002273:Carcinogens; D009153:Mutagens