title: [Effect of glycyrrhetinic acid on the expression of inflammatory factors in fibroblast-like synovial cells from collagen induced arthritis rats].
abstract: OBJECTIVE
  To determine the effects of glycyrrhetinic acid (GA), methotrexate(MTX) and GA+MTX on the expressions of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in fibroblast-like synovial (FLS) cells from collagen induced arthritis (CIA) rats.
  
  
  METHODS
  CIA-FLS cells were isolated and purified from the synovium of CIA rats by tissue block  culture method, and then treated with MTX, GA, GA+MTX or nothing (control group), respectively. Each group was cultured for 3 different time periods (1, 3, 5 d). Real-time qPCR was used to detect the expressions of TNF-α and IL-1β mRNA in CIA-FLS cells and ELISA to determine the levels of TNF-α and IL-1β in the supernatant.
  
  
  RESULTS
  Day 1: No significant down-regulation of TNF-α and IL-1β mRNA was identified in intervention groups compared with the control group (P>0.05); Day 3: Intervention groups significantly down-regulated TNF-α and IL-1β mRNA (P<0.05 vs control), but without intergroup difference(P>0.05); Day 5: the three intervention groups all suppressed the expressions of TNF-α and IL-1β mRNA(P<0.05 vs control), with GA+MTX>MTX>GA in inhibiting effect (P<0.05). All intervention groups were found to inhibit the expressions of TNF-α and IL-1β in a time-dependent manner (P<0.05). Similar outcomes were observed in the change of the levels of TNF-α and IL-1β in cell culture supernatant by ELISA.
  
  
  CONCLUSIONS
  GA, MTX, GA+MTX suppress the expression of TNF-α and IL-1β in CIA-FLS cells in a time-dependent manner, and the suppressing effect is GA+MTX>MTX>GA.
authors.affiliation: Department of Nephrology, Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gui
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000378:metabolism; D002469:Cell Separation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism; D006034:Glycyrrhetinic Acid / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000032:analysis* / Q000235:genetics; D008297:Male; D008727:Methotrexate / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013582:Synovial Fluid / Q000166:cytology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000235:genetics
chemical_list: D053583:Interleukin-1beta; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D006034:Glycyrrhetinic Acid; D008727:Methotrexate