title: Allografts of the acellular sciatic nerve and brain-derived neurotrophic factor repair spinal cord injury in adult rats.
abstract: OBJECTIVE
  We aimed to investigate whether an innovative growth factor-laden scaffold composed of acellular sciatic nerve (ASN) and brain-derived neurotrophic factor (BDNF) could promote axonal regeneration and functional recovery after spinal cord injury (SCI).
  
  
  METHODS
  Following complete transection at the thoracic level (T9), we immediately transplanted the grafts between the stumps of the severed spinal cords. We evaluated the functional recovery of the hindlimbs of the operated rats using the BBB locomotor rating scale system every week. Eight weeks after surgery, axonal regeneration was examined using the fluorogold (FG) retrograde tracing method. Electrophysiological analysis was carried out to evaluate the improvement in the neuronal circuits. Immunohistochemistry was employed to identify local injuries and recovery.
  
  
  RESULTS
  The results of the Basso-Beattie-Bresnahan (BBB) scale indicated that there was no significant difference between the individual groups. The FG retrograde tracing and electrophysiological analyses indicated that the transplantation of ASN-BDNF provided a permissive environment to support neuron regeneration.
  
  
  CONCLUSIONS
  The ASN-BDNF transplantation provided a promising therapeutic approach to promote axonal regeneration and recovery after SCI, and can be used as part of a combinatory treatment strategy for SCI management.
authors.affiliation: Key Laboratory in Cell Transplantation in Ministry of Health of China, Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Li
authors.forename: Xiangtong
authors.initials: X
authors.lastname: Zhang
authors.forename: Ronglong
authors.initials: R
authors.lastname: Cao
authors.forename: Bohai
authors.initials: B
authors.lastname: Yu
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Liang
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Dayong
authors.initials: D
authors.lastname: Li
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Wang
authors.forename: Enzhong
authors.initials: E
authors.lastname: Liu
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology; D001485:Basement Membrane / Q000473:pathology; D001812:Blood-Brain Barrier; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D004594:Electrophysiology / Q000379:methods; D005260:Female; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D009416:Nerve Regeneration; D009474:Neurons / Q000378:metabolism; D051381:Rats; D012584:Sciatic Nerve / Q000503:physiopathology* / Q000648:ultrastructure; D013116:Spinal Cord / Q000473:pathology; D013119:Spinal Cord Injuries / Q000628:therapy*; D014184:Transplantation, Homologous
chemical_list: D019208:Brain-Derived Neurotrophic Factor