title: An insight into the influence of low dose irradiation pretreatment on the microbial decolouration and degradation of Reactive Red-120 dye.
abstract: The influence of low dose irradiation pretreatment on the microbial decolouration and degradation of Reactive Red-120 (RR-120) dye was investigated in detail by using Pseudomonas sp. SUK1. About 27%, 56% and 66% decolouration of 150 ppm RR-120 dye solution was observed by applying 0, 0.5 and 1 kGy doses, respectively, in the first step followed by microbial treatment for 24 h under static condition. Similarly, about 70%, 88% and 90% TOC removal was observed by applying 0, 0.5 and 1 kGy doses, respectively, in the first step followed by the microbial treatment for 96 h under static condition. The radiation induced fragmented products of RR-120 at doses of 0.5 and 1 kGy were investigated by FTIR and electrospray ionization-MS analysis. The induction of the enzymes viz. laccase, tyrosinase, azoreductase and NADH-2,6-dichlorophenol indophenol reductase was studied in the decolourised solution obtained after irradiating 150 ppm RR-120 dye solution with 0 and 1 kGy doses followed by the microbial treatment for 96 h under static condition. The enzymatic degradation products were studied by FTIR, HPLC and GC-MS. The toxicity study of the treated dye solution on plants revealed the degradation of RR-120 into non-toxic products by combined radiation-microbial treatment. This study explores a reliable and promising way to use industrially viable dose (≤1 kGy) and microbial strain viz. Pseudomonas sp. SUK1 for permissible safe disposal of dye solutions from textile industries.
authors.affiliation: Radiation Technology Development Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India. paul.jhimli@gmail.com
authors.forename: Jhimli
authors.initials: J
authors.lastname: Paul
authors.forename: Avinash A
authors.initials: AA
authors.lastname: Kadam
authors.forename: Sanjay P
authors.initials: SP
authors.lastname: Govindwar
authors.forename: Pranaw
authors.initials: P
authors.lastname: Kumar
authors.forename: Lalit
authors.initials: L
authors.lastname: Varshney
mesh_terms: D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005720:Gamma Rays; D007220:Industrial Waste / Q000032:analysis*; D011549:Pseudomonas / Q000378:metabolism / Q000528:radiation effects*; D011829:Radiation Dosage; D017550:Spectroscopy, Fourier Transform Infrared; D013783:Textile Industry; D014227:Triazines / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D007220:Industrial Waste; D014227:Triazines; D014874:Water Pollutants, Chemical; C042516:reactive red 120 dye