title: Sustained protection against photoreceptor degeneration in tubby mice by intravitreal injection of nanoceria.
abstract: We previously reported that nanoceria can slow retinal degeneration in the tubby mouse for two weeks by multiple systemic injections. However, the long-term protection of retinal structure and function by directly deliver of nanoceria to the eye had not been explored. In this study, 172 ng of nanoceria in 1 μl saline (1 mm) were intravitreally injected into tubby P7 pups and assays were performed at P28, P49, P80 and P120. The expression of antioxidant associated genes and photoreceptor-specific genes was significantly up regulated, the mislocalization of rod and cone opsins was decreased, and retinal structure and function were protected. These findings demonstrate that nanoceria can function as catalytic antioxidants in vivo and may be broad spectrum therapeutic agents for multiple types of ocular diseases.
authors.affiliation: Department of Ophthalmology, Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. xue-cai@ouhsc.edu
authors.forename: Xue
authors.initials: X
authors.lastname: Cai
authors.forename: Steven A
authors.initials: SA
authors.lastname: Sezate
authors.forename: Sudipta
authors.initials: S
authors.lastname: Seal
authors.forename: James F
authors.initials: JF
authors.lastname: McGinnis
mesh_terms: D000818:Animals; D002563:Cerium / Q000008:administration & dosage / Q000627:therapeutic use*; D005786:Gene Expression Regulation / Q000187:drug effects; D058449:Intravitreal Injections; D051379:Mice; D008810:Mice, Inbred C57BL; D055355:Opsins / Q000032:analysis / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects; D020419:Photoreceptor Cells, Vertebrate / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012160:Retina / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D017949:Retinal Cone Photoreceptor Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D012162:Retinal Degeneration / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology / Q000517:prevention & control*; D017948:Retinal Rod Photoreceptor Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D055355:Opsins; D002563:Cerium; C030583:ceric oxide