title: Evaluation of a sticky trap (AedesTraP), made from disposable plastic bottles, as a monitoring tool for Aedes aegypti populations.
abstract: BACKGROUND
  Dengue virus, which is transmitted by Aedes aegypti mosquitoes is the most important emerging viral disease, infecting more than 50 million people annually. Currently used sticky traps are useful tools for monitoring and control of A. aegypti, despite differences in efficiency, labor requirements and cost. In the present work, a field assay was carried out to evaluate the performance of a sticky trap (AedesTrap), produced using disposable material, in capturing gravid Aedes spp. females. Additionally, conditions necessary for the improved performance of the device, such as number of traps per site and location (indoors or outdoors) were evaluated.
  
  
  METHODS
  During a one year period, traps were placed in a dengue endemic area in 28 day cycles. The trap, named AedesTrap, consisted of a disposable plastic soda bottle coated inside with colophony resin, which served as a sticky substrate. Disposable bottles were donated by restaurants, and traps were made by laboratory staff, reducing the cost of the sticky trap (less than U$3). Mosquito capture in indoor and outdoor areas was compared by placing the traps in laundry room, kitchen or bedroom (indoors) and front or back yard (outdoors). The relationship between the number of AedesTraps and quantity of captured mosquitoes was investigated by utilizing one or three traps/site.
  
  
  RESULTS
  During a 28 day cycle, a single AedesTrap was capable of capturing up to 15 A. aegypti in a house, with a mean capture of 0.5 to 2.63 females per premise. The AedesTrap collected three times more outdoors versus indoors. Similarly, the capability of detecting Aedes spp. infestation, and of capturing females, was three times higher when using three AedesTraps per house, compared with one trap per house.
  
  
  CONCLUSIONS
  AedesTrap was shown to be capable of capturing A. aegypti and other culicidae, providing information on the adult mosquito population, and allowing the identification of areas critically infested by mosquitoes. Low requirements for skilled labor together with easy maintenance and low cost are additional advantages of using this sticky trap.
authors.affiliation: Laboratory of Terrestrial Invertebrates, Department of Zoology, Center of Biological Sciences, Universidade Federal de Pernambuco, Av, Moraes Rego s/n, Cidade Universitária, Recife, PE, CEP: 50670-420, Brazil. eloina.santos@gmail.com
authors.forename: Eloína Maria Mendonça
authors.initials: EM
authors.lastname: de Santos
authors.forename: Maria Alice Varjal
authors.initials: MA
authors.lastname: de Melo-Santos
authors.forename: Claudia Maria Fontes
authors.initials: CM
authors.lastname: de Oliveira
authors.forename: Juliana Cavalcanti
authors.initials: JC
authors.lastname: Correia
authors.forename: Cleide Maria Ribeiro
authors.initials: CM
authors.lastname: de Albuquerque
mesh_terms: D000330:Aedes / Q000502:physiology*; D000818:Animals; D003715:Dengue / Q000517:prevention & control*; D005260:Female; D007303:Insect Vectors / Q000502:physiology*; D009032:Mosquito Control / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data*; D010969:Plastics; D011157:Population Dynamics / Q000706:statistics & numerical data; D012116:Resins, Plant; D013048:Specimen Handling / Q000295:instrumentation* / Q000379:methods
chemical_list: D010969:Plastics; D012116:Resins, Plant; C013893:rosin