title: Effect of low culture temperature on urokinase production in hollow fiber reactor.
abstract: The effect of culture temperature on urokinase production by HT-1080 cell line was studied in batch culture and hollow fiber reactor. Small-scale t-flask experiments revealed that urokinase production could be enhanced and media utilization could be reduced by lowering the culture temperature in production phase. Urokinase production was scaled up using a hollow fiber perfusion reactor system. Temperature of culture was maintained at the physiological 37 °C during growth phase that extended up to 12 days in hollow fiber bioreactor. Subsequently, in the production phase, culture temperature was lowered to 34 °C. Decrease in culture temperature resulted in a significant increase in urokinase production. Proteolytic degradation and inhibition was also minimized. The medium utilization rate was decreased at lower temperature, and hence, a higher economy of production could be obtained.
authors.affiliation: Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110 016, India. shilpas.fet@mriu.edu.in
authors.forename: Shilpa S
authors.initials: SS
authors.lastname: Khaparde
authors.forename: Pradip K
authors.initials: PK
authors.lastname: Roychoudhury
mesh_terms: D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D019149:Bioreactors; D045744:Cell Line, Tumor; D003080:Cold Temperature; D006801:Humans; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis*
chemical_list: D014568:Urokinase-Type Plasminogen Activator