title: Annexin A5 protein expression is associated with the histological differentiation             of uterine cervical squamous cell carcinoma in patients with an increased serum             concentration.
abstract: Annexin A5 (ANXA5) is a calcium-dependent phospholipid-binding protein belonging             to the annexin family and is expressed abnormally in several types of carcinoma.             In the present study, ANXA5 protein expression was evaluated by western blot analysis             in a series of 60 human uterine cervical squamous cell carcinomas (UCSCCs) to             search for molecular alterations that may be able to serve as useful diagnostic/prognostic             markers. The upregulation of ANXA5 expression was observed in 48/60 UCSCC cases             (80%), whereas a weak expression was observed in the 25 normal uterine cervical             tissues. ANXA5 expression was also analyzed by immunohistochemical staining, western             blot and reverse transcription-polymerase chain reaction (RT-PCR) assays of the             UCSCC and uterine cervical normal tissue lesions. All dysplastic tissues showed             significantly increased ANXA5 expression compared with the weak signal observed             in normal epithelia. A close association was observed between the ANXA5 expression             levels and the histological grade of UCSCC. Compared with moderately and well-differentiated             tumors, there was a significant increase in ANXA5 expression in poorly differentiated             tumors. Furthermore, ANXA5 concentrations in the blood serum of the patients were             significantly increased. Our findings clearly identify ANXA5 as an effective differentiation             marker for the histopathological grading of UCSCCs and for the detection of epithelial             dysplasia. The results from our study support the critical role of ANXA5 in the             molecular profiling of UCSCC.
authors.affiliation: Basic Research Institution, Chengde Medical College, Chengde,                     P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.forename: Xiu Jun
authors.initials: XJ
authors.lastname: Liang
authors.forename: Yu Feng
authors.initials: YF
authors.lastname: Gao
authors.forename: Xiao Jie
authors.initials: XJ
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Yan
authors.forename: Fu Lu
authors.initials: FL
authors.lastname: Gao
mesh_terms: D017304:Annexin A5 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000097:blood; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D060787:Neoplasm Grading; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology
chemical_list: D017304:Annexin A5; D000951:Antigens, Neoplasm; D012333:RNA, Messenger