title: Biochemical analysis of pentraxin 3 and fibrinogen levels in experimental periodontitis model.
abstract: OBJECTIVE
  Pentraxin 3 (PTX3), newly discovered inflammation marker, is a member of acute-phase proteins. The hypothesis, synthesis of gingival tissue and serum PTX-3 increases in the experimental periodontitis model (with 10-day and 40-day periods), was tested by detecting gingival tissue and serum PTX-3 levels in rats with experimental periodontitis.
  
  
  METHODS
  Thirty rats were randomly divided into three groups of ten animals each: ligature-induced experimental periodontitis groups (with 10-day (Group1) and 40-day periods (Group2)) and healthy group (Group3). At the end of experimental period, rats were sacrificed, and radiological and histomorphometric analyses were performed on the mandibles. PTX3 levels were measured in gingival tissue and serum samples using ELISA. Plasma fibrinogen levels were measured according to the nephelometric method.
  
  
  RESULTS
  Significant alveolar bone resorption and periodontal inflammation were evident in periodontitis groups. Levels of PTX3 in gingival tissue were statistically higher in Group 1 than those in groups 2 and 3 (P < 0.01). No significant difference was found in serum PTX3 levels between experimental periodontitis and control groups (P > 0.05). Plasma fibrinogen levels were significantly increased in the experimental periodontitis groups (P < 0.001).
  
  
  CONCLUSIONS
  PTX3 seems to be associated with tissue destruction in earlier periods of inflammatory periodontal disease, contrary to the fibrinogen findings.
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Ondokuzmayis University, 55139 Samsun, Turkey. goncakeles@hotmail.com
authors.forename: Gonca Cayir
authors.initials: GC
authors.lastname: Keles
authors.forename: Umut
authors.initials: U
authors.lastname: Balli
authors.forename: Burcu Ozkan
authors.initials: BO
authors.lastname: Cetinkaya
authors.forename: Bulent
authors.initials: B
authors.lastname: Ayas
authors.forename: Arzu
authors.initials: A
authors.lastname: Findik
authors.forename: Zeynep Pinar
authors.initials: ZP
authors.lastname: Keles
authors.forename: Ferda
authors.initials: F
authors.lastname: Pamuk
mesh_terms: D000818:Animals; D002097:C-Reactive Protein / Q000378:metabolism*; D003238:Connective Tissue / Q000378:metabolism; D004195:Disease Models, Animal; D005340:Fibrinogen / Q000378:metabolism*; D008297:Male; D010518:Periodontitis / Q000378:metabolism*; D010519:Periodontium / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000683:Serum Amyloid P-Component / Q000378:metabolism*; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D005340:Fibrinogen; D000683:Serum Amyloid P-Component; D000099153:Pentraxins