title: Removal of 2,4,5-trichlorophenol by bacterial isolates from the secondary sludge of pulp and paper mill.
abstract: 2,4,5-trichlorophenol (2,4,5-TCP) mineralizing bacteria were isolated from the secondary sludge of pulp and paper industry. These isolates used 2,4,5-TCP as a source of carbon and energy and were capable of degrading this compound, as indicated by stoichimetric release of chloride and biomass formation. Based on 16S rRNA sequence analysis, these bacteria were identified as Kocuria sp. (CL2), Bacillus pumillus (CL5), Pseudomonas stutzeri (CL7). HPLC analysis revealed that these isolates were able to degrade 2,4,5-TCP at higher concentrations (600 mg/l or 3.0 mM). A consortia of these isolates completely removed 2,4,5-TCP from the sludge obtained from pulp and paper mill within 2 weeks when supplemented at a rate of 100 mg l(-1) . Bacterial consortium also significantly reduced absorbable organic halogen (AOX) and extractable organic halogen (EOX) by 61% and 63%, respectively from the sludge. These isolates have high potential to remove 2,4,5-TCP and may be used for remediation of pulp paper mill waste containing 2,4,5-TCP.
authors.affiliation: Department of Biotechnology, Thapar University, Patiala, 147 004, Punjab, India.
authors.forename: Santosh Kr
authors.initials: SK
authors.lastname: Karn
authors.forename: M Sudhakara
authors.initials: MS
authors.lastname: Reddy
mesh_terms: D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002733:Chlorophenols / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004734:Energy Metabolism; D004785:Environmental Pollutants / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D007220:Industrial Waste; D059013:Microbial Consortia; D008835:Micrococcaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D044223:Pseudomonas stutzeri / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D002733:Chlorophenols; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004785:Environmental Pollutants; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; C009534:2,4,5-trichlorophenol; D002244:Carbon