title: Characterization of a diffuse intrinsic pontine glioma cell line: implications for future investigations and treatment.
abstract: Diffuse intrinsic pontine gliomas arise almost exclusively in children, and despite advances in treatment, the majority of patients die within 2 years after initial diagnosis. Because of their infiltrative nature and anatomic location in an eloquent area of the brain, most pontine gliomas are treated without a surgical biopsy. The corresponding lack of tissue samples has resulted in a limited understanding of the underlying genetic and molecular biologic abnormalities associated with pontine gliomas, and is a substantial obstacle for the preclinical testing of targeted therapeutic agents for these tumors. We have established a human glioma cell line that originated from surgical biopsy performed on a patient with a pontine glioma. To insure sustainable in vitro propagation, tumor cells were modified with hTERT (human telomerase ribonucleoprotein reverse transcriptase), and with a luciferase reporter to enable non-invasive bioluminescence imaging. The hTERT modified cells are tumorigenic in athymic rodents, and produce brainstem tumors that recapitulate the infiltrative growth of brainstem gliomas in patients.
authors.affiliation: Department of Neurological Surgery, Brain Tumor Research Center, University of California San Francisco, San Francisco, CA 94143-0520, USA.
authors.forename: Rintaro
authors.initials: R
authors.lastname: Hashizume
authors.forename: Ivan
authors.initials: I
authors.lastname: Smirnov
authors.forename: Sharon
authors.initials: S
authors.lastname: Liu
authors.forename: Joanna J
authors.initials: JJ
authors.lastname: Phillips
authors.forename: Jeanette
authors.initials: J
authors.lastname: Hyer
authors.forename: Tracy R
authors.initials: TR
authors.lastname: McKnight
authors.forename: Michael
authors.initials: M
authors.lastname: Wendland
authors.forename: Michael
authors.initials: M
authors.lastname: Prados
authors.forename: Anu
authors.initials: A
authors.lastname: Banerjee
authors.forename: Theodore
authors.initials: T
authors.lastname: Nicolaides
authors.forename: Sabine
authors.initials: S
authors.lastname: Mueller
authors.forename: Charles D
authors.initials: CD
authors.lastname: James
authors.forename: Nalin
authors.initials: N
authors.lastname: Gupta
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D020295:Brain Stem Neoplasms / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D002648:Child; D005260:Female; D020869:Gene Expression Profiling; D005910:Glioma / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011149:Pons; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011923:Rats, Nude; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger