title: Immunoaffinity, capillary electrophoresis, and statistics for studying intact alpha 1-acid glycoprotein isoforms as an atherothrombosis biomarker.
abstract: Variations in the amino acid sequence, glycosylation, and/or other posttranslational modifications in glycoproteins give rise to different molecules of the glycoprotein called forms. Qualitative and/or quantitative alterations in these forms are related to pathophysiological situations in the individuals. In this study, a methodology to analyze these differences in forms of the alpha 1-acid glycoprotein (AGP) between healthy individuals and patients with two different vascular diseases is detailed. The whole methodology includes a sample preparation method based on immunochromatography, a capillary electrophoresis method for separation of AGP peaks (isoforms), and statistical methods (Linear Discriminant Analysis) for sample classification. As a result, it is shown that the methodology proposed allows studying the role of AGP isoforms as potential vascular disease biomarkers.
authors.affiliation: Institute of Organic Chemistry (IQOG-CSIC), Madrid, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Puerta
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Martin-Alvarez
authors.forename: Sara
authors.initials: S
authors.lastname: Ongay
authors.forename: Jose C
authors.initials: JC
authors.lastname: Diez-Masa
authors.forename: Mercedes
authors.initials: M
authors.lastname: de Frutos
mesh_terms: D050197:Atherosclerosis / Q000175:diagnosis*; D015415:Biomarkers / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods*; D016002:Discriminant Analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D009961:Orosomucoid / Q000302:isolation & purification / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D012015:Reference Standards; D013223:Statistics as Topic; D013927:Thrombosis / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D009961:Orosomucoid; D020033:Protein Isoforms