title: Purification of naringin and neohesperidin from Huyou (Citrus changshanensis) fruit and their effects on glucose consumption in human HepG2 cells.
abstract: Huyou (Citrus changshanensis) is rich in naringin and neohesperidin, which are natural flavanone glycosides with a range of biological activities. Among the different fruit parts, i.e. flavedo, albedo, segment membrane (SM), and juice sacs (JS), albedo showed the highest contents of both compounds, with 27.00 and 19.09mg/g DW for naringin and neohesperidin, respectively. Efficient simultaneous purification of naringin and neohesperidin from Huyou albedo was established by the combination of macroporous D101 resin chromatography and high-speed counter-current chromatography (HSCCC). Purified naringin and neohesperidin were identified by both HPLC and LC-MS, and their effects on glucose consumption were investigated in HepG2 cells. Cells treated with naringin and neohesperidin showed increased consumption of glucose, and this was associated with increased phosphorylation of AMP-activated protein kinase (AMPK). Therefore, naringin and neohesperidin from Huyou may act as potential hypoglycaemic agents through regulation of glucose metabolism.
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, PR China.
authors.forename: Jiukai
authors.initials: J
authors.lastname: Zhang
authors.forename: Chongde
authors.initials: C
authors.lastname: Sun
authors.forename: Youyou
authors.initials: Y
authors.lastname: Yan
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Chen
authors.forename: Fenglei
authors.initials: F
authors.lastname: Luo
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhu
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Kunsong
authors.initials: K
authors.lastname: Chen
mesh_terms: D002957:Citrus / Q000737:chemistry*; D044950:Flavanones / Q000302:isolation & purification* / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D005947:Glucose / Q000378:metabolism*; D056945:Hep G2 Cells; D006569:Hesperidin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D044950:Flavanones; D010936:Plant Extracts; D006569:Hesperidin; D005947:Glucose; C005274:naringin; C546526:neohesperidin