title: Dynamics of detachment of Escherichia coli from plasma-mediated coatings under shear flow.
abstract: A series of plasma-mediated coatings, containing silver nanoparticles embedded in an organosilicon or silica-like matrix, were deposited onto stainless steel and chemically characterized. Their anti-adhesive properties were evaluated in vitro towards Escherichia coli by performing shear-flow induced detachment experiments. Increasing the wall shear stress facilitated E. coli cell detachment, irrespective of the coating characteristics. When nanosilver was incorporated, cell detachment was lower, probably due to the affinity of the embedded silver for biological components of the cell wall. The presence of methyl groups in the matrix network could also promote enhanced hydrophobic interactions. Within the population fraction remaining attached to the coating under increasing shear flow, different association phenotypes were observed, viz. progressively lying flat, moving laterally, remaining tethered, or rotating by a single anchoring point, until alignment with the flow direction. This re-orientation phenotype and its relation with detachment were dependent of the coating. The effects of such heterogeneities should be more deeply explored.
authors.affiliation: Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, Toulouse, F-31077, France. muriel.mercier-bonin@insa-toulouse.fr
authors.forename: Muriel
authors.initials: M
authors.lastname: Mercier-Bonin
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Duviau
authors.forename: Christophe
authors.initials: C
authors.lastname: Ellero
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lebleu
authors.forename: Patrice
authors.initials: P
authors.lastname: Raynaud
authors.forename: Bernard
authors.initials: B
authors.lastname: Despax
authors.forename: Philippe
authors.initials: P
authors.lastname: Schmitz
mesh_terms: D001422:Bacterial Adhesion; D004926:Escherichia coli / Q000254:growth & development / Q000502:physiology*; D033081:Shear Strength; D012834:Silver / Q000737:chemistry; D013193:Stainless Steel; D013499:Surface Properties
chemical_list: D013193:Stainless Steel; D012834:Silver