title: Let-7a inhibits proliferation and induces apoptosis by targeting EZH2             in nasopharyngeal carcinoma cells.
abstract: Let-7a is frequently downregulated in various types of human cancer including             nasopharyngeal carcinoma. However, the underlying mechanism of let-7a action in             nasopharyngeal carcinoma remains elusive. In this study, we show that the enhancer             of zeste homolog 2 (EZH2) is a direct target of let-7a in human nasopharyngeal             carcinoma cells. The inhibition of EZH2 in vitro by let-7a, EZH2 siRNA, attenuated             nasopharyngeal carcinoma cell growth, inhibited cell proliferation and induced             cell apoptosis. In addition, for each biological process we identified ontology-associated             transcripts that significantly correlate with EZH2 expression. Finally, the expression             of EZH2 significantly abrogated let-7a-mediated cell proliferation and apoptosis             in the nasopharyngeal carcinoma cells. Taken together, our results suggest that             let-7a and EZH2 may be potential therapeutic targets for nasopharyngeal carcinoma.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Taizhou People's Hospital, Taizhou 225300, Jiangsu, PR China. entcaikemin7173@126.com
authors.forename: Kemin
authors.initials: K
authors.lastname: Cai
authors.forename: Yi
authors.initials: Y
authors.lastname: Wan
authors.forename: Guan
authors.initials: G
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Xueli
authors.initials: X
authors.lastname: Bao
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D002277:Carcinoma; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D000071221:Enhancer of Zeste Homolog 2 Protein; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D063151:Polycomb Repressive Complex 2 / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering; D014407:Tumor Cells, Cultured
chemical_list: D004268:DNA-Binding Proteins; D035683:MicroRNAs; D034741:RNA, Small Interfering; C487931:mirnlet7 microRNA, human; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein; D063151:Polycomb Repressive Complex 2