title: Effects of sterilization and storage on the properties of ALP-grafted biomaterials for prosthetic and bone tissue engineering applications.
abstract: Grafting of the biomaterial surfaces with biomolecules is nowadays a challenging research field for prosthetic and bone tissue engineering applications. On the other hand, very few research works investigate the effect of the sterilization processes on the properties of functionalized biomaterials. In this study, the effects of different sterilization techniques (e.g. gamma and electron beam irradiation, ethylene oxide) on the enzymatic activity of bioactive glasses and Ti6Al4V grafted with alkaline phosphatase (ALP) have been analyzed. Sterility maintenance and in vitro bioactivity of the sterilized surfaces have also been investigated. Finally the effect of packaging and storage conditions has been considered.
authors.affiliation: Politecnico di Torino, Department of Applied Science and Technology, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy. sara.ferraris@polito.it
authors.forename: S
authors.initials: S
authors.lastname: Ferraris
authors.forename: G
authors.initials: G
authors.lastname: Pan
authors.forename: C
authors.initials: C
authors.lastname: Cassinelli
authors.forename: L
authors.initials: L
authors.lastname: Mazzucco
authors.forename: E
authors.initials: E
authors.lastname: Vernè
authors.forename: S
authors.initials: S
authors.lastname: Spriano
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000528:radiation effects; D000497:Alloys; D001610:Beta Particles; D001672:Biocompatible Materials / Q000737:chemistry* / Q000528:radiation effects; D018786:Bone Substitutes / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000528:radiation effects; D005027:Ethylene Oxide; D005720:Gamma Rays; D005898:Glass / Q000528:radiation effects; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D019736:Prostheses and Implants; D013242:Sterilization / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods*; D014025:Titanium / Q000528:radiation effects
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; C031462:titanium alloy (TiAl6V4); D014025:Titanium; D000469:Alkaline Phosphatase; D005027:Ethylene Oxide