title: Structure and characteristics of acid and pepsin-solubilized collagens from the skin of cobia (Rachycentron canadum).
abstract: Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the skin of cobia (Rachycentron canadum). The yields of ASC and PSC were 35.5% and 12.3%, respectively. Based on the protein patterns and carboxymethyl-cellulose chromatography, ASC and PSC were composed of α1α2α3 heterotrimers and were characterised as type I collagen with no disulfide bond. Their amounts of imino acids were 203 and 191 residues per 1000 residues, respectively. LC-MS/MS analysis demonstrated the high sequences similarities of ASC and PSC. Fourier transform infrared spectroscopy spectra showed that the amide I, II and III peaks of PSC were obtained at a lower wave number compared with ASC. The thermal denaturation temperatures of ASC and PSC, as measured by viscometry, were 34.62 and 33.97°C, respectively. The transition temperatures (T(max)) were 38.17 and 36.03°C, respectively, as determined by differential scanning calorimetry (DSC). Both collagens were soluble at acidic pH and below 2% (w/v) NaCl concentration.
authors.affiliation: College of Food Science & Technology, Guangdong Ocean University, Zhanjiang 524088, China.
authors.forename: Shaokui
authors.initials: S
authors.lastname: Zeng
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Yin
authors.forename: Shuqi
authors.initials: S
authors.lastname: Yang
authors.forename: Chaohua
authors.initials: C
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.forename: Wenlong
authors.initials: W
authors.lastname: Wu
mesh_terms: D000143:Acids / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D029941:Fish Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010434:Pepsin A / Q000737:chemistry; D010473:Perciformes / Q000378:metabolism*; D055550:Protein Stability; D012867:Skin / Q000737:chemistry* / Q000378:metabolism; D012995:Solubility
chemical_list: D000143:Acids; D029941:Fish Proteins; D003094:Collagen; D010434:Pepsin A