title: Changes in lipid transport-involved proteins of epicardial adipose tissue associated with coronary artery disease.
abstract: OBJECTIVE
  Recent studies have focused on the potential role of epicardial adipose tissue (EAT) in the physiopathology of several metabolic and cardiovascular diseases, especially coronary artery disease (CAD). We aimed to study whether there are differences in the proteome and the secretome between epicardial and subcutaneous adipose tissue (SAT) from patients with and without CAD.
  
  
  METHODS
  EAT and SAT samples were collected from 64 patients undergoing elective cardiac surgery either for coronary artery bypass grafting or valve surgery. One or two-dimensional electrophoresis were performed on tissue samples and media collected at 3, 6, 24 or 48 of tissue culture. Protein identification was performed with mass spectrometry, and the results were then validated with Western blot or enzyme immunoassay. mRNA expression levels were analysed by real time polymerase chain reaction.
  
  
  RESULTS
  The release of several proteins was found to be higher in EAT that in SAT. Remarkably, there were higher levels of apolipoprotein A-I and glutation S-transferase P release, whereas mRNA expression of fatty acid binding protein 4 was lower in EAT. Although apolipoprotein A-I protein quantity in EAT was similar between CAD and non CAD patients, its released levels from this fat pad were lower in CAD.
  
  
  CONCLUSIONS
  EAT and SAT show different profiles of protein release and a different pattern was also found in samples from patients with CAD. These findings might support the hypothesis that EAT plays an interesting role in the physiopathology of atherosclerosis and CAD.
authors.affiliation: Cardiovascular Division, Health Research Institute, University Clinical Hospital of Santiago de Compostela, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Salgado-Somoza
authors.forename: Elvis
authors.initials: E
authors.lastname: Teijeira-Fernández
authors.forename: Ángel Luis
authors.initials: ÁL
authors.lastname: Fernández
authors.forename: José Ramón
authors.initials: JR
authors.lastname: González-Juanatey
authors.forename: Sonia
authors.initials: S
authors.lastname: Eiras
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000368:Aged; D016632:Apolipoprotein A-I / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D015153:Blotting, Western; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000235:genetics / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D051549:Glutathione S-Transferase pi / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D050356:Lipid Metabolism / Q000235:genetics; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D010496:Pericardium / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050151:Subcutaneous Fat / Q000378:metabolism; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I; D017077:Culture Media, Conditioned; C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi