title: Dual-color imaging of magnesium/calcium ion activities with two-photon fluorescent probes.
abstract: We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg(2+) ([Mg(2+)](i)) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca(2+) ([Ca(2+)](m)), FMg2 allows dual-color imaging of Mg(2+)/Ca(2+) activities in live cells and [Mg(2+)](i) /[Ca(2+)](m) distributions in live tissues at a depth of 100-200 μm.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Dong
authors.forename: Ji Hee
authors.initials: JH
authors.lastname: Han
authors.forename: Cheol Ho
authors.initials: CH
authors.lastname: Heo
authors.forename: Hwan Myung
authors.initials: HM
authors.lastname: Kim
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Liu
authors.forename: Bong Rae
authors.initials: BR
authors.lastname: Cho
mesh_terms: D002118:Calcium / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D007477:Ions / Q000032:analysis; D008274:Magnesium / Q000032:analysis*; D036641:Microscopy, Fluorescence, Multiphoton; D015394:Molecular Structure; D017785:Photons
chemical_list: D005456:Fluorescent Dyes; D007477:Ions; D008274:Magnesium; D002118:Calcium