title: Adipose-derived stem cells induced dendritic cells undergo tolerance and inhibit Th1 polarization.
abstract: Adipose tissue-derived stem cells (ADSC) have been shown to possess stem cell properties such as transdifferentiation, self-renewal and therapeutic potential. However, the property of ADSC to accommodate immune system is still unknown. In this study, ADSC were cocultured with allogenetic dendritic cells (DC), and then treated DC were mixed with allogenetic CD4+ T cells. The results demonstrated that ADSC could downregulate costimulatory molecules, including CD80, CD83, CD86, and cytokine secretion such as interleukin (IL)-12 and tumor necrosis factor (TNF)-α, while upregulate indoleamine-2,3-dioxygenase (IDO) of allogenetic DC. In addition, treated DC could inhibit CD4+ T cell activation and naïve T cells toward Th1 polarization. The results suggest that ADSC could negatively modulate immunity and induce immune tolerance, which provide a promising strategy in transplantation or autoimmune disease.
authors.affiliation: Department 4, Research Institute of Field Surgery, The 3rd Military Medical University, State Key Lab of Trauma, Burns and Combined Injury, 400042 Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.forename: Tianjun
authors.initials: T
authors.lastname: Gao
authors.forename: Zai-Liang
authors.initials: ZL
authors.lastname: Yang
authors.forename: Shi-Chang
authors.initials: SC
authors.lastname: Zhang
authors.forename: Ming-Liang
authors.initials: ML
authors.lastname: Ren
authors.forename: Zheng-Guo
authors.initials: ZG
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D000328:Adult; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002450:Cell Communication / Q000235:genetics / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007108:Immune Tolerance; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics / Q000276:immunology; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D008875:Middle Aged; D061251:Primary Cell Culture; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D015703:Antigens, CD; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12