title: Characterization of Bombyx mori nucleopolyhedrovirus Bm17.
abstract: Open reading frame17 (Bm17) of Bombyx mori nucleopolyhedrovirus is a highly conserved gene in lepidopteran nucleopolyhedroviruses, suggesting that it performs an important role in the virus life cycle whose function is unknown. In this report, we describe the characterization of Bm17. Reversed transcriptive-PCR (RT-PCR) and Western blot analysis demonstrated that Bm17 was expressed as a late gen. Immunofluorescence analysis by confocal microscopy showed that BM17 protein was localized on cytoplasm and nucleus of infected cells. These results show that BM17 was a late protein localized in cytoplasm and nucleus.
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, P. R. China; School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, P. R. China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Shen
authors.forename: Rudu
authors.initials: R
authors.lastname: Wang
authors.forename: Qinggong
authors.initials: Q
authors.lastname: Han
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Nin
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Shihe
authors.initials: S
authors.lastname: Shao
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000821:virology*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins