title: Cloning and genomic nucleotide sequence of the matrix attachment region binding protein from the halotolerant alga Dunaliella salina.
abstract: In our previous study, the sequence of a matrix attachment region binding protein (MBP) cDNA was cloned from the unicellular green alga Dunaliella salina. However, the nucleotide sequence of this gene has not been reported so far. In this paper, the nucleotide sequence of MBP was cloned and characterized, and its gene copy number was determined. The MBP nucleotide sequence is 5641 bp long, and interrupted by 12 introns ranging from 132 to 562 bp. All the introns in the D. salina MBP gene have orthodox splice sites, exhibiting GT at the 5' end and AG at the 3' end. Southern blot analysis showed that MBP only has one copy in the D. salina genome.
authors.affiliation: Sino-British Research Centre for Molecular Oncology, Zhengzhou University, Zhengzhou, Henan, PR, China.
authors.forename: Peng-Ju
authors.initials: PJ
authors.lastname: Wang
authors.forename: Tian-Yun
authors.initials: TY
authors.lastname: Wang
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.forename: Zhao-Xi
authors.initials: ZX
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D015139:Blotting, Southern; D000460:Chlorophyta / Q000378:metabolism*; D003001:Cloning, Molecular; D048028:DNA, Algal / Q000737:chemistry / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018745:Genome, Plant; D023281:Genomics; D007438:Introns; D036961:Matrix Attachment Region Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D045170:Matrix Attachment Regions; D008969:Molecular Sequence Data
chemical_list: D048028:DNA, Algal; D018076:DNA, Complementary; D005656:Fungal Proteins; D036961:Matrix Attachment Region Binding Proteins