title: Differential gene expression between skin and cervix induced by the E7 oncoprotein in a transgenic mouse model.
abstract: HPV16 E7 oncoprotein expression in K14E7 transgenic mice induces cervical cancer after 6 months of treatment with the co-carcinogen 17β-estradiol. In untreated mice, E7 also induces skin tumors late in life albeit at low penetrance. These findings indicate that E7 alters cellular functions in cervix and skin so as to predispose these organs to tumorigenesis. Using microarrays, we determined the global genes expression profile in cervical and skin tissue of young adult K14E7 transgenic mice without estrogen treatment. In these tissues, the E7 oncoprotein altered the transcriptional pattern of genes involved in several biological processes including signal transduction, transport, metabolic process, cell adhesion, apoptosis, cell differentiation, immune response and inflammatory response. Among the E7-dysregulated genes were ones not previously known to be involved in cervical neoplasia including DMBT1, GLI1 and 17βHSD2 in cervix, as well as MMP2, 12, 14, 19 and 27 in skin.
authors.affiliation: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados, México DF, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Ibarra Sierra
authors.forename: J
authors.initials: J
authors.lastname: Díaz Chávez
authors.forename: E M
authors.initials: EM
authors.lastname: Cortés-Malagón
authors.forename: L
authors.initials: L
authors.lastname: Uribe-Figueroa
authors.forename: A
authors.initials: A
authors.lastname: Hidalgo-Miranda
authors.forename: P F
authors.initials: PF
authors.lastname: Lambert
authors.forename: P
authors.initials: P
authors.lastname: Gariglio
mesh_terms: D000818:Animals; D001483:Base Sequence; D002584:Cervix Uteri / Q000378:metabolism / Q000821:virology; D004195:Disease Models, Animal; D005260:Female; D005814:Genes, Viral; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000502:physiology; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008822:Mice, Transgenic; D020411:Oligonucleotide Array Sequence Analysis; D050725:Papillomavirus E7 Proteins / Q000235:genetics* / Q000502:physiology*; D030361:Papillomavirus Infections / Q000209:etiology / Q000235:genetics / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism / Q000821:virology; D059467:Transcriptome; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000235:genetics / Q000821:virology
chemical_list: D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; C059731:oncogene protein E7, Human papillomavirus type 16