title: Discovery of a novel circular single-stranded DNA virus from porcine faeces.
abstract: A large number of novel single-stranded DNA (ssDNA) viruses have been characterised from various environmental sources in the last 5 years. The bulk of these have been from faecal sources, and faecal sampling is an ideal non-invasive pathogen sampling method. We characterised a novel ssDNA from a porcine faecal sample from Cass Basin of the South Island of New Zealand. The novel viral genome has two large open reading frames (ORFs), which are bidirectionally transcribed and separated by intergenic regions. The largest ORF has some degree of similarity (<30 %) to the putative capsid protein of chimpanzee stool-associated circular ssDNA virus (ChiSCV) and pig stool-associated single-stranded DNA virus (PigSCV), whereas the second-largest ORF has high similarity to the putative replication-associated protein (Rep) of ChiSCV (~50 %) and bovine stool-associated circular DNA virus (BoSCV; ~30 %). Based on genome architecture, location of putative stem-loop like elements, and maximum-likelihood phylogenetic analysis of the gene encoding the Rep protein, the novel isolate belongs to the same family of ssDNA viruses as ChiSCV and BoSCV.
authors.affiliation: School of Biological Sciences, University of Canterbury, Ilam, Private Bag 4800, Christchurch 8140, New Zealand.
authors.forename: Alyssa
authors.initials: A
authors.lastname: Sikorski
authors.forename: Gerardo R
authors.initials: GR
authors.lastname: Argüello-Astorga
authors.forename: Anisha
authors.initials: A
authors.lastname: Dayaram
authors.forename: Renwick C J
authors.initials: RC
authors.lastname: Dobson
authors.forename: Arvind
authors.initials: A
authors.lastname: Varsani
mesh_terms: D000818:Animals; D004267:DNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005243:Feces / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D013552:Swine
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral