title: Nanogap-based electrical PNA chips for the detection of genetic polymorphism of cytochrome P450 2C19.
abstract: PNA chips for the detection of the genetic polymorphism of Cytochrome P450 2C19 (CYP2C19), a well-known enzyme related to the metabolism of therapeutic drugs, were electrically-interfaced with interdigitated nanogap electrodes (INEs). The average gap distance and effective length of the INEs were about approximately 70 nm and approximately 140/m, respectively. Those INEs having the aspect ratio of about 2000, were prepared by the combination of the photolithography (for the formation of initial electrodes) and the surface-catalyzed chemical deposition (for the gap narrowing), without the e-beam lithography. The PNA probes for the detection of CYP2C19 were immobilized in the gap region of INEs via Schiff base formation. The I-V characteristics clearly showed a sharp increase in the conductance between the nanogap electrodes upon the PNA-DNA hybridization, followed by the adsoprtion of functionalized Au nanoparticles. Four different target DNAs for the diagnosis of CYP2C19 polymorphism were successfully detected and discriminated with the INE-based PNA chips.
authors.affiliation: Department of Chemistry, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Dae Keun
authors.initials: DK
authors.lastname: Park
authors.forename: Hyung Ju
authors.initials: HJ
authors.lastname: Park
authors.forename: Cho Yeon
authors.initials: CY
authors.lastname: Lee
authors.forename: Daewha
authors.initials: D
authors.lastname: Hong
authors.forename: Young
authors.initials: Y
authors.lastname: Lee
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
authors.forename: Wan Soo
authors.initials: WS
authors.lastname: Yun
mesh_terms: D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics*; D003217:Conductometry / Q000295:instrumentation*; D065731:Cytochrome P-450 CYP2C19; D004252:DNA Mutational Analysis / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D036103:Nanotechnology / Q000295:instrumentation*; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
chemical_list: D001189:Aryl Hydrocarbon Hydroxylases; C045793:CYP2C19 protein, human; D065731:Cytochrome P-450 CYP2C19