title: Rationally-designed fluorescent lysine riboswitch probes.
abstract: Two fluorescent lysine amide analogs, in which the carboxyl end of lysine was covalently attached to dansyl or NBD groups through an ethylene glycol-based linker, were rationally designed and synthesized. Both probes showed high binding affinity to the lysine riboswitch in vitro and their fluorescence intensities decreased by riboswitch binding.
authors.affiliation: Department of Chemistry, Texas Christian University, 2800 S. University Dr., Fort Worth, TX 76109, USA.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Budhathoki
authors.forename: Lina F
authors.initials: LF
authors.lastname: Bernal-Perez
authors.forename: Onofrio
authors.initials: O
authors.lastname: Annunziata
authors.forename: Youngha
authors.initials: Y
authors.lastname: Ryu
mesh_terms: D000577:Amides / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry*; D015394:Molecular Structure; D058928:Riboswitch
chemical_list: D000577:Amides; D005456:Fluorescent Dyes; D058928:Riboswitch; D008239:Lysine