title: RNA-Seq of early-infected poplar leaves by the rust pathogen Melampsora larici-populina uncovers PtSultr3;5, a fungal-induced host sulfate transporter.
abstract: Biotroph pathogens establish intimate interactions with their hosts that are conditioned by the successful secretion of effectors in infected tissues and subsequent manipulation of host physiology. The identification of early-expressed pathogen effectors and early-modulated host functions is currently a major goal to understand the molecular basis of biotrophy. Here, we report the 454-pyrosequencing transcriptome analysis of early stages of poplar leaf colonization by the rust fungus Melampsora larici-populina. Among the 841,301 reads considered for analysis, 616,879 and 649 were successfully mapped to Populus trichocarpa and M. larici-populina genome sequences, respectively. From a methodological aspect, these results indicate that this single approach is not appropriate to saturate poplar transcriptome and to follow transcript accumulation of the pathogen. We identified 19 pathogen transcripts encoding early-expressed small-secreted proteins representing candidate effectors of interest for forthcoming studies. Poplar RNA-Seq data were validated by oligoarrays and quantitatively analysed, which revealed a highly stable transcriptome with a single transcript encoding a sulfate transporter (herein named PtSultr3;5, POPTR_0006s16150) showing a dramatic increase upon colonization by either virulent or avirulent M. larici-populina strains. Perspectives connecting host sulfate transport and biotrophic lifestyle are discussed.
authors.affiliation: Unité Mixte de Recherche 1136 'Interactions Arbres/Microorganismes', INRA (Institut National de la Recherche Agronomique)/Université de Lorraine, Centre INRA de Nancy, Champenoux, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Petre
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Morin
authors.forename: Emilie
authors.initials: E
authors.lastname: Tisserant
authors.forename: Stéphane
authors.initials: S
authors.lastname: Hacquard
authors.forename: Corinne
authors.initials: C
authors.lastname: Da Silva
authors.forename: Julie
authors.initials: J
authors.lastname: Poulain
authors.forename: Christine
authors.initials: C
authors.lastname: Delaruelle
authors.forename: Francis
authors.initials: F
authors.lastname: Martin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rouhier
authors.forename: Annegret
authors.initials: A
authors.lastname: Kohler
authors.forename: Sébastien
authors.initials: S
authors.lastname: Duplessis
mesh_terms: D001487:Basidiomycota / Q000502:physiology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D054884:Host-Pathogen Interactions / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D032107:Populus / Q000235:genetics* / Q000382:microbiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013431:Sulfates / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics
chemical_list: D026901:Membrane Transport Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D013431:Sulfates