title: Recapitulating tumour microenvironment in chitosan-gelatin three-dimensional scaffolds: an improved in vitro tumour model.
abstract: Owing to the reduced co-relationship between conventional flat Petri dish culture (two-dimensional) and the tumour microenvironment, there has been a shift towards three-dimensional culture systems that show an improved analogy to the same. In this work, an extracellular matrix (ECM)-mimicking three-dimensional scaffold based on chitosan and gelatin was fabricated and explored for its potential as a tumour model for lung cancer. It was demonstrated that the chitosan-gelatin (CG) scaffolds supported the formation of tumoroids that were similar to tumours grown in vivo for factors involved in tumour-cell-ECM interaction, invasion and metastasis, and response to anti-cancer drugs. On the other hand, the two-dimensional Petri dish surfaces did not demonstrate gene-expression profiles similar to tumours grown in vivo. Further, the three-dimensional CG scaffolds supported the formation of tumoroids, using other types of cancer cells such as breast, cervix and bone, indicating a possible wider potential for in vitro tumoroid generation. Overall, the results demonstrated that CG scaffolds can be an improved in vitro tool to study cancer progression and drug screening for solid tumours.
authors.affiliation: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, Uttar Pradesh, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Arya
authors.forename: Viren
authors.initials: V
authors.lastname: Sardana
authors.forename: Meera
authors.initials: M
authors.lastname: Saxena
authors.forename: Annapoorni
authors.initials: A
authors.lastname: Rangarajan
authors.forename: Dhirendra S
authors.initials: DS
authors.lastname: Katti
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005780:Gelatin / Q000737:chemistry*; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000473:pathology; D008954:Models, Biological; D017382:Reactive Oxygen Species / Q000378:metabolism; D054457:Tissue Scaffolds; D059016:Tumor Microenvironment
chemical_list: D000970:Antineoplastic Agents; D017382:Reactive Oxygen Species; D005780:Gelatin; D048271:Chitosan