title: Biosynthesis of anti-HCV compounds using thermophilic microorganisms.
abstract: This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2'-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379mg/L of 6-chloropurine-2'-deoxyriboside. The obtained compounds can be used as antiviral agents.
authors.affiliation: Laboratorio de Investigaciones en Biotecnología Sustentable (LIBioS), Universidad Nacional de Quilmes. Roque Saenz Peña 352, Bernal (B1868BXD), Argentina.
authors.forename: Cintia W
authors.initials: CW
authors.lastname: Rivero
authors.forename: Eliana C
authors.initials: EC
authors.lastname: De Benedetti
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Sambeth
authors.forename: Mario E
authors.initials: ME
authors.lastname: Lozano
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Trelles
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D001411:Geobacillus stearothermophilus / Q000737:chemistry / Q000378:metabolism*; D016174:Hepacivirus / Q000187:drug effects*; D011684:Purine Nucleosides / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology*; D013696:Temperature
chemical_list: C577882:6-chloropurine-2'-deoxyriboside; D000998:Antiviral Agents; D011684:Purine Nucleosides; C024008:6-chloropurine riboside