title: Relationship of urinary isoprostanes to prostate cancer occurrence.
abstract: To estimate the oxidative stress in patients with prostate cancer and in a control group, we used the biomarker of lipid peroxidation-isoprostanes (8-isoPGF(2)) and the level of selected antioxidants (glucose and uric acid [UA]). The level of urinary isoprostanes was determined in patients and controls using an immunoassay kit according to the manufacturer's instruction. The levels of UA and glucose were also determined in serum by the use of UA Assay Kit and Glucose Assay Kit. We observed a statistically increased the level of isoprostanes in urine of patients with prostate cancer in compared with a control group. The concentration of tested antioxidants in blood from patients with prostate cancer was also higher than in healthy subjects. Moreover, our experiments indicate that the correlation between the increased amount of UA and the lipid peroxidation exists in prostate cancer patients (in all tested groups). Prostate cancer risk by urinary isoprostanes level was analyzed, and a positive association was found (relative risk for highest vs. lowest quartile of urinary isoprostanes = 1.6; 95 % confidence interval 1.2-2.4; p for trend = 0.03). We suggest that reactive oxygen species induce peroxidation of unsaturated fatty acid in patients with prostate cancer, and the level of isoprostanes may be used as a non-invasive marker for determination of oxidative stress. We also propose that UA may enhance the oxidative stress in patients with prostate cancer.
authors.affiliation: Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Brys
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Morel
authors.forename: Ewa
authors.initials: E
authors.lastname: Forma
authors.forename: Anna
authors.initials: A
authors.lastname: Krzeslak
authors.forename: Jacek
authors.initials: J
authors.lastname: Wilkosz
authors.forename: Waldemar
authors.initials: W
authors.lastname: Rozanski
authors.forename: Beata
authors.initials: B
authors.lastname: Olas
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000652:urine*; D001786:Blood Glucose; D016022:Case-Control Studies; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009929:Organ Size; D018384:Oxidative Stress; D011467:Prostate / Q000473:pathology; D011471:Prostatic Neoplasms / Q000097:blood / Q000473:pathology / Q000652:urine*; D012306:Risk; D014527:Uric Acid / Q000097:blood
chemical_list: D014408:Biomarkers, Tumor; D001786:Blood Glucose; D014527:Uric Acid; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost