title: Cytokine analysis by immunoaffinity capillary electrophoresis.
abstract: Immunoaffinity capillary electrophoresis (ICE) is a powerful tool used to detect and quantify target proteins of interest in complex biological fluids. The target analyte is captured and bound to antibodies immobilized onto the wall of a capillary, labeled in situ with a fluorescent dye, eluted and detected online using laser-induced fluorescence following electrophoretic separation. Here, we illustrate how to construct an immunoaffinity capillary and utilize it to run ICE in order to capture and quantify target cytokines and chemokines from a clinical sample.
authors.affiliation: Micro Analytical Immunochemistry Unit, Biomedical Engineering and Physical Science Shared Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Mendonca
authors.forename: Heather
authors.initials: H
authors.lastname: Kalish
mesh_terms: D000906:Antibodies / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D016207:Cytokines / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis; D012015:Reference Standards; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D000906:Antibodies; D016207:Cytokines; D007140:Immunoglobulin Fab Fragments; D011994:Recombinant Proteins; D013438:Sulfhydryl Compounds; D014409:Tumor Necrosis Factor-alpha