title: Detection of viable Mycobacterium ulcerans in clinical samples by a novel combined 16S rRNA reverse transcriptase/IS2404 real-time qPCR assay.
authors.affiliation: Department of Infectious Diseases and Tropical Medicine, University Hospital, Ludwig-Maximilians University, Munich, Germany. beissner@lrz.uni-muenchen.de
authors.forename: Marcus
authors.initials: M
authors.lastname: Beissner
authors.forename: Dominik
authors.initials: D
authors.lastname: Symank
authors.forename: Richard Odame
authors.initials: RO
authors.lastname: Phillips
authors.forename: Yaw Ampem
authors.initials: YA
authors.lastname: Amoako
authors.forename: Nana-Yaa
authors.initials: NY
authors.lastname: Awua-Boateng
authors.forename: Fred Stephen
authors.initials: FS
authors.lastname: Sarfo
authors.forename: Moritz
authors.initials: M
authors.lastname: Jansson
authors.forename: Kristina Lydia
authors.initials: KL
authors.lastname: Huber
authors.forename: Karl-Heinz
authors.initials: KH
authors.lastname: Herbinger
authors.forename: Florian
authors.initials: F
authors.lastname: Battke
authors.forename: Thomas
authors.initials: T
authors.lastname: Löscher
authors.forename: Ohene
authors.initials: O
authors.lastname: Adjei
authors.forename: Gisela
authors.initials: G
authors.lastname: Bretzel
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques / Q000379:methods; D054312:Buruli Ulcer / Q000175:diagnosis*; D002648:Child; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods; D019911:Mycobacterium ulcerans / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S