title: Chromolithic method development, validation and system suitability analysis of ultra-sound assisted extraction of glycyrrhizic acid and glycyrrhetinic acid from Glycyrrhiza glabra.
abstract: An ultrasound-assisted extraction and chromolithic LC method was developed for simultaneous determination of glycyrrhizic acid (GA) and glycyrrhetinic acid (GL) from the root extract of Glycyrrhizza glabra using RPLC-PDA. The developed method was validated according to the International Conference on Harmonisation. The method exhibited good linearity (r2 > 0.9989) with high precision and achieved good accuracies between 97.5 to 101.3% of quantitative results. The method is more sensitive and faster (resolved within ten minutes) than the earlier developed methods using normal LC columns.
authors.affiliation: Plant Biotechnology Division, Indian Institute of Integrative Medicine, Jammu-180001, Jammu & Kashmir, India.
authors.forename: Suphla
authors.initials: S
authors.lastname: Gupta
authors.forename: Rajni
authors.initials: R
authors.lastname: Sharma
authors.forename: Pankaj
authors.initials: P
authors.lastname: Pandotra
authors.forename: Sundeep
authors.initials: S
authors.lastname: Jaglan
authors.forename: Ajai Prakash
authors.initials: AP
authors.lastname: Gupta
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006034:Glycyrrhetinic Acid / Q000737:chemistry*; D006035:Glycyrrhiza / Q000737:chemistry*; D019695:Glycyrrhizic Acid / Q000737:chemistry*; D015394:Molecular Structure; D015203:Reproducibility of Results; D014465:Ultrasonics
chemical_list: D019695:Glycyrrhizic Acid; D006034:Glycyrrhetinic Acid