title: Enhanced expression of IL-10 in contrast to IL-12B mRNA in poultry with experimental coccidiosis.
abstract: Interleukin (IL)-12 and IL-10 are immunoregulatory cytokines with an antagonistic effect on the T-helper (Th)1/Th2 cytokine balance and they provide a functional link between innate resistance and the adaptive immune response. This investigation was conducted to determine the expression of IL-10 and IL-12B mRNA levels in chickens' gut mucosa infected with Eimeria tenella and in sulfachlorpyrazine-sodium treated animals after infection. Broiler chickens were randomly allocated in three groups: healthy untreated control; infected untreated animals and infected, treated with sulfachlorpyrazine sodium chickens 6 days after the challenge with an E. tenella. Quantitative real time PCR analysis was performed using specific primer pairs and probes for IL-10 and IL-12B. The expression of IL-10 mRNA was greater in the duodenum then in the caecum and the liver of healthy chickens. E. tenella infection led to significant up-regulation of IL-10 mRNA in the caecum, followed by mRNA in the liver. A significant down regulation was observed mainly in the caecum after the treatment with sulfachlorpyrazine. In contrast, IL-12B expression in all investigated tissues remained insignificantly affected in the studied groups of animals. Distinct up-regulation of IL-10 mRNA, after the challenge with E. tenella, in the caecum can be attributed to the tissue tropism of Eimeria spp. The production of IL-12 is regulated by negative feedback through IL-10 which explains lack of increase in IL-12B mRNA. Sulfonamide treatment resulted in clinical improvement and restoration of IL-10 mRNA to the levels observed in healthy chickens.
authors.affiliation: Department of Pharmacology, Physiology of Animals and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria. haritova@uni-sz.bg
authors.forename: A M
authors.initials: AM
authors.lastname: Haritova
authors.forename: S A
authors.initials: SA
authors.lastname: Stanilova
mesh_terms: D000818:Animals; D002432:Cecum / Q000276:immunology / Q000469:parasitology; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000188:drug therapy / Q000276:immunology / Q000662:veterinary*; D003049:Coccidiostats / Q000627:therapeutic use; D004386:Duodenum / Q000276:immunology / Q000469:parasitology; D016786:Eimeria tenella / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000276:immunology / Q000469:parasitology; D008297:Male; D011201:Poultry Diseases / Q000188:drug therapy / Q000276:immunology* / Q000469:parasitology; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D016053:RNA, Protozoan / Q000302:isolation & purification / Q000378:metabolism; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013424:Sulfanilamides / Q000627:therapeutic use; D015854:Up-Regulation; D000096926:Benzenesulfonamides
chemical_list: D003049:Coccidiostats; D016753:Interleukin-10; D018664:Interleukin-12; D012333:RNA, Messenger; D016053:RNA, Protozoan; D013424:Sulfanilamides; C050643:sulfachlorpyrazine; D000096926:Benzenesulfonamides