title: In vivo evaluation of porous hydroxyapatite/chitosan-alginate composite scaffolds for bone tissue engineering.
abstract: Porous hydroxyapatite (HAp)/chitosan-alginate composite scaffolds were prepared through in situ co-precipitation and freeze-drying for bone tissue engineering. The composite scaffolds were highly porous and interconnected with a pore size of around 50-220 μm at low concentrations of HAp. As the HAp content increased, the porosity of the scaffolds decreased from 84.98 to 74.54%. An MTT assay indicates that the obtained scaffolds have no cytotoxic effects on MG-63 cells, and that they have good biocompatibility. An implantation experiment in mouse skulls revealed that the composite scaffold provides a strong positive effect on bone formation in vivo in mice. Furthermore, that HAp/chitosan-alginate composite scaffold has been shown to be more effective for new bone generation than chitosan-alginate scaffold.
authors.affiliation: School of Materials Science and Engineering, Pusan National University, San 30, Jangjeon-dong, Gumjeong-gu, Busan 609-735, Republic of Korea.
authors.forename: Hyeong-Ho
authors.initials: HH
authors.lastname: Jin
authors.forename: Dong-Hyun
authors.initials: DH
authors.lastname: Kim
authors.forename: Tae-Wan
authors.initials: TW
authors.lastname: Kim
authors.forename: Keun-Koo
authors.initials: KK
authors.lastname: Shin
authors.forename: Jin Sup
authors.initials: JS
authors.lastname: Jung
authors.forename: Hong-Chae
authors.initials: HC
authors.lastname: Park
authors.forename: Seog-Young
authors.initials: SY
authors.lastname: Yoon
mesh_terms: D000464:Alginates / Q000378:metabolism*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D001842:Bone and Bones / Q000166:cytology* / Q000502:physiology; D045744:Cell Line, Tumor; D048271:Chitosan / Q000378:metabolism*; D017886:Durapatite / Q000378:metabolism*; D005612:Freeze Drying; D020723:Glucuronic Acid / Q000378:metabolism; D006603:Hexuronic Acids / Q000378:metabolism; D006801:Humans; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D048271:Chitosan; D017886:Durapatite