title: Periodontal health and serum, saliva matrix metalloproteinases in patients with mild chronic obstructive pulmonary disease.
abstract: OBJECTIVE
  The present case-control study aimed to evaluate comparatively the salivary and serum levels of matrix metalloproteinases (MMP)-8 and- 13 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in patients with mild chronic obstructive pulmonary disease (COPD) and non-COPD controls.
  
  
  METHODS
  Clinical periodontal measurements were recorded before any periodontal intervention in 36 patients with mild COPD and 20 non-COPD controls admitted to Ege University Department of Chest Diseases COPD outpatient clinic (İzmir, Turkey). Salivary and serum levels of MMP-8, MMP-13, and TIMP-1 were determined by immunofluorometric assay (IFMA) and enzyme-linked immunosorbent assay (ELISA). Data were analyzed with non-parametric statistical tests.
  
  
  RESULTS
  Patients with COPD were significantly older than the control group (p < 0.05). The COPD group showed significantly higher serum levels of MMP-8 IFMA, MMP-8/TIMP-1 IFMA than the control group (p < 0.005). By ELISA, serum MMP-8, MMP-8/TIMP-1, TIMP-1, and MMP-13 levels were similar in both groups (p > 0.05). Salivary MMP-8, MMP-13, and TIMP-1 levels were similar in both groups (p > 0.05).
  
  
  CONCLUSIONS
  The present findings suggest that immunodetection of MMP-8 is dependent on the selected techniques and even with mild COPD some systemic inflammatory markers such as MMP-8 tend to increase. However, the present clinical periodontal and biochemical findings do not provide support for the previously proposed interaction between COPD and periodontal diseases.
authors.affiliation: Department of Periodontology, School of Dentistry, Ege University, İzmir, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Yıldırım
authors.forename: I
authors.initials: I
authors.lastname: Kormi
authors.forename: Ö K
authors.initials: ÖK
authors.lastname: Başoğlu
authors.forename: A
authors.initials: A
authors.lastname: Gürgün
authors.forename: B
authors.initials: B
authors.lastname: Kaval
authors.forename: T
authors.initials: T
authors.lastname: Sorsa
authors.forename: N
authors.initials: N
authors.lastname: Buduneli
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D055113:Chronic Periodontitis / Q000097:blood / Q000150:complications* / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000032:analysis / Q000097:blood*; D020784:Matrix Metalloproteinase 8 / Q000032:analysis / Q000097:blood*; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000150:complications* / Q000201:enzymology*; D012463:Saliva / Q000201:enzymology*; D013147:Spirometry; D018709:Statistics, Nonparametric; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000032:analysis / Q000097:blood
chemical_list: D019715:Tissue Inhibitor of Metalloproteinase-1; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8