title: Association between TNFRSF11B gene polymorphisms and history of ischemic stroke in Italian diabetic patients.
abstract: Osteoprotegerin (OPG) is a secretory glycoprotein that belongs to the tumor necrosis factor receptor family and plays a role in atherosclerosis. The present study aimed to evaluate whether OPG gene (TNFRSF11B) polymorphisms are involved in ischemic stroke in an Italian population with diabetes. Participants in a retrospective case-control study included 364 diabetic patients (180 males, 184 females) with history of ischemic stroke and 492 diabetic subjects without history of ischemic stroke (252 males, 240 females). The T245G, T950C, and G1181C polymorphisms of the OPG gene were analyzed by polymerase chain reaction and restriction fragment length polymorphism. We found that the T245G, T950C, and G1181C gene polymorphisms of the OPG gene were significantly (34.1 vs. 9.5 %, P < 0.0001; 30.8 vs. 6.3 %, P < 0.0001 and 26.4 vs. 11.6 % P < 0.0001, respectively) and independently (adjusted OR 5.15 [3.46-7.68], OR 6.63 [4.26-10.31], and OR 3.03 [2.04-4.50], respectively) associated with history of ischemic stroke. We also found that these three polymorphisms act synergistically in patients with stroke history. The TNFRSF11B gene polymorphisms studied are associated with history of ischemic stroke and synergistic effects between these genotypes might be potential markers for cerebrovascular disorders.
authors.affiliation: Department of Medicine, A. Gemelli University Hospital, Catholic University School of Medicine, Rome, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Biscetti
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Straface
authors.forename: Silvia
authors.initials: S
authors.lastname: Giovannini
authors.forename: Angelo
authors.initials: A
authors.lastname: Santoliquido
authors.forename: Flavia
authors.initials: F
authors.lastname: Angelini
authors.forename: Luca
authors.initials: L
authors.lastname: Santoro
authors.forename: Carlo Filippo
authors.initials: CF
authors.lastname: Porreca
authors.forename: Giovanni
authors.initials: G
authors.lastname: Pecorini
authors.forename: Giovanni
authors.initials: G
authors.lastname: Ghirlanda
authors.forename: Andrea
authors.initials: A
authors.lastname: Flex
mesh_terms: D000368:Aged; D001483:Base Sequence; D016022:Case-Control Studies; D004247:DNA / Q000235:genetics; D048909:Diabetes Complications / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D007558:Italy; D008297:Male; D053244:Osteoprotegerin / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012189:Retrospective Studies; D012307:Risk Factors; D020521:Stroke / Q000150:complications* / Q000235:genetics*
chemical_list: D053244:Osteoprotegerin; C506030:TNFRSF11B protein, human; D004247:DNA