title: The biological effects of fibrin-binding synthetic oligopeptides derived from fibronectin on osteoblast-like cells.
abstract: OBJECTIVE
  The aim of this study was to investigate the effects of synthetic fibronectin (FN) fragments, including fibrin binding sites from amino-terminal FN fragments containing type I repeats 1 to 5, on osteoblast-like cell activity.
  
  
  METHODS
  Oligopeptides ranging from 9 to 20 amino acids, designated FF1, FF3, and FF5, were synthesized by a solid-phase peptide synthesizing system, and we investigated the effects of these peptides on cell attachment and extent of mineralization using confocal microscopy, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, and Alizarin red S staining.
  
  
  RESULTS
  FF3 and FF5 peptides increased the number of attached human osteoblastic cells, and FF3 administration led to prominent cell spreading. Mineralization was increased in FF3 and FF5 compared to FF1 and the untreated control.
  
  
  CONCLUSIONS
  Taken together, it can be concluded that the fibrin-binding oligopeptides FF3 and FF5 enhanced cell attachment and mineralization on osteoblast-like cells. These results indicate that FF3 and FF5 have the potential to increase osteoblast-like cell activity. Performing an in vivo study may provide further possibilities for surface modification of biomimetic peptides to enhance osteogenesis, thus improving the regeneration of destroyed alveolar bone.
authors.affiliation: Department of Periodontology, Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.
authors.forename: Yun-Jeong
authors.initials: YJ
authors.lastname: Kim
authors.forename: Yoon-Jeong
authors.initials: YJ
authors.lastname: Park
authors.forename: Yong-Moo
authors.initials: YM
authors.lastname: Lee
authors.forename: In-Chul
authors.initials: IC
authors.lastname: Rhyu
authors.forename: Young
authors.initials: Y
authors.lastname: Ku