title: Preparative, in vitro biocatalysis of triketide lactone chiral building blocks.
abstract: PKS biocatalysis: The terminal module of erythromycin synthase was used for the in vitro production of chiral triketide lactones. Combining cofactor regeneration, substrate truncation, and enzymatic promiscuity afforded a scalable strategy to generate these molecules from abundant racemic and achiral precursors. The described biocatalytic platform thus facilitates the application and study of enzymes within PKS modules.
authors.affiliation: Institute for Cellular and Molecular Biology, University of Texas at Austin, 1 University Station, A4800, Austin, TX 78712, USA.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Harper
authors.forename: Constance B
authors.initials: CB
authors.lastname: Bailey
authors.forename: Angela D
authors.initials: AD
authors.lastname: Edwards
authors.forename: Joshua F
authors.initials: JF
authors.lastname: Detelich
authors.forename: Adrian T
authors.initials: AT
authors.lastname: Keatinge-Clay
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D055162:Biocatalysis; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D007783:Lactones / Q000737:chemistry / Q000378:metabolism*; D048630:Polyketide Synthases / Q000737:chemistry / Q000378:metabolism*; D013237:Stereoisomerism; D048372:Streptomyces coelicolor / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D007783:Lactones; D048630:Polyketide Synthases