title: Increased prostaglandin E₂-EP2 signalling in cumulus cells of female mice sired by males with the Y-chromosome long-arm deletion.
abstract: Cumuli oophori surrounding ovulated oocytes of B10.BR(Y(del)) females (sired by males with the Y-chromosome long-arm deletion) are more resistant to hyaluronidase digestion than cumuli oophori around eggs of genetically identical females but sired by males with the intact Y chromosome (B10.BR). This has been interpreted as a result of differences in paternal genome imprinting, which females of both groups inherit from their fathers. The following study shows that it is not hyaluronan, but rather excessive protein concentration, that makes the cumulus extracellular matrix of B10.BR(Y(del)) oocytes more resistant to enzymatic treatment. It was revealed, additionally, that cumulus cells around ovulating oocytes of B10.BR(Y(del)) females display higher surface accumulation of prostaglandin EP2 subtype receptors and higher expression of the Ptgs2 gene (encoding a rate-limiting enzyme of prostaglandin E₂ synthesis) in relation to the cells of control B10.BR females. The expression levels of the prostaglandin-dependent Tnfaip6 and Ccl2 genes were also altered in B10.BR(Y(del)) cumulus cells in a manner indicating increased prostaglandin signalling. The study provides further evidence for the divergence in reproductive phenotypes between B10.BR and B10.BR(Y(del)) female mice. It supports the hypothesis that genes of the Y-chromosome long arm may be involved in establishment of epigenetic marks in X-bearing spermatozoa.
authors.affiliation: Department of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland. katarzyna.kotarska@uj.edu.pl
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kotarska
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Lenartowicz
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Przybyło
authors.forename: Aniela
authors.initials: A
authors.lastname: Gołas
authors.forename: Józefa
authors.initials: J
authors.lastname: Styrna
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics; D054885:Cumulus Cells / Q000166:cytology / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis* / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D018392:Genomic Imprinting; D007248:Infertility, Male; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D006080:Ovarian Follicle / Q000166:cytology / Q000378:metabolism; D010060:Ovulation; D058307:Receptors, Prostaglandin E, EP2 Subtype / Q000235:genetics* / Q000378:metabolism; D012729:Sex Chromosome Aberrations; D058533:Sex Chromosome Disorders of Sex Development / Q000235:genetics*; D015398:Signal Transduction; D013499:Surface Properties; D015854:Up-Regulation
chemical_list: C491768:Ccl2 protein, mouse; D015815:Cell Adhesion Molecules; D018932:Chemokine CCL2; C545645:Ptger2 protein, mouse; D058307:Receptors, Prostaglandin E, EP2 Subtype; C513057:Tnfaip6 protein, mouse; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015232:Dinoprostone