title: Purification and characterization of a novel peptide with inhibitory effects on colitis induced mice by dextran sulfate sodium from enzymatic hydrolysates of Crassostrea gigas.
abstract: The anti-inflammatory activity of purified peptides from Crassostrea gigas (C. gigas) hydrolysates was studied. To prepare hydrolysates from C. gigas, we used eight different proteinases and the anti-inflammatory activities were determined using a nitric oxide (NO) assay in RAW264.7 cells. Among the hydrolysates, Protamex hydrolysates showed the highest anti-inflammatory activity. We separated and purified the total hydrolysate using an ultrafiltration membrane system and consecutive chromatographic methods. Finally, we obtained a peptide with the following sequence: Gln-Cys-Gln-Cys-Ala-Val-Glu-Gly-Gly-Leu at N-terminal position. The anti-inflammatory peptide purified from C. gigas inhibited NO production by 72.2% compared to the lipopolysaccharide (LPS) treated group. In addition, the Protamex hydrolysates from C. gigas showed decreased serum IgE levels and increased spleen CD4(+)/CD8(+) levels on dextran sulfate sodium (DSS) induced colitis in mice. These results suggest the peptide and hydrolysate from C. gigas possess potent anti-inflammatory effect.
authors.affiliation: Department of Biotechnology, Konkuk University, Chungju 380-701, Republic of Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Hwang
authors.forename: Seung-Jae
authors.initials: SJ
authors.lastname: Lee
authors.forename: Yon-Suk
authors.initials: YS
authors.lastname: Kim
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Ahn
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Moon
authors.forename: Byong-Tae
authors.initials: BT
authors.lastname: Jeon
authors.forename: Pyo-Jam
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D049896:Crassostrea / Q000737:chemistry*; D016264:Dextran Sulfate / Q000633:toxicity; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000037:antagonists & inhibitors*; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D020539:Sequence Analysis, Protein
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D009842:Oligopeptides; D009569:Nitric Oxide; D016264:Dextran Sulfate