title: Effects of single swim stress on changes in TRPV1-mediated plasticity in the amygdala.
abstract: By examining the involvement of transient receptor potential vanilloid type 1 (TRPV1) in the stress modulation of learning and memory processes in mice, we evaluated the effects of endovanilloid N-oleoyldopamine (OLDA) on the long-term potentiation (LTP) of the lateral nucleus of the amygdala (LA). After high-frequency stimulation of external capsule fibers we found that LA-LTP is reduced in OLDA-treated slices derived from adult C57BL/6 control mice. The specificity of the TRPV1 receptor activation by OLDA was confirmed by blocking the OLDA-induced inhibitory effect on LA-LTP with the specific TRPV1 receptor antagonist AMG 9810. The specificity of OLDA was further supported by using TRPV1 deficient mice, where the effect of OLDA on LA-LTP was missing. Following exposure to a forced swim test (FST) OLDA enhanced LA-LTP in control but not TRPV1-deficient mice. The results also show that a short period of acute stress significantly impairs LA-LTP. Since we have recently shown the involvement of cannabinoid CB1 receptors in the mediation of capsaicin-induced inhibitory effects on LA-LTP ([23] Zschenderlein et al., 2011), it is reasonable to assume that the OLDA-induced enhancement of LA-LTP after the forced swim test can be attributed to the up-regulation of TRPV1 and the action of ligands such as anandamide on TRPV1. As a result, stimulation of TRPV1 receptors rescues LTP in slices derived from swim-stressed mice.
authors.affiliation: Institute of Neurophysiology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Kulisch
authors.affiliation: Institute of Neurophysiology, Charité-Universitätsmedizin Berlin, Berlin, Germany. Electronic address: doris.albrecht@charite.de.
authors.forename: Doris
authors.initials: D
authors.lastname: Albrecht
mesh_terms: D000178:Acrylamides / Q000494:pharmacology; D000679:Amygdala / Q000503:physiopathology*; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology; D004298:Dopamine / Q000031:analogs & derivatives / Q000494:pharmacology; D004558:Electric Stimulation; D005110:Extracellular Space / Q000502:physiology; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009473:Neuronal Plasticity / Q000502:physiology*; D013315:Stress, Psychological / Q000503:physiopathology* / Q000523:psychology*; D013550:Swimming / Q000523:psychology*; D050916:TRPV Cation Channels / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: C500530:3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo(b)(1,4)dioxin-6-yl)acrylamide; D000178:Acrylamides; D019086:Bridged Bicyclo Compounds, Heterocyclic; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D002211:Capsaicin; C488796:N-oleoyldopamine; D004298:Dopamine