title: Rapid identification of Chikungunya and Dengue virus by a real-time reverse transcription-loop-mediated isothermal amplification method.
abstract: Both Chikungunya and Dengue virus belong to the acute arthropod-borne viruses. Because of the lack of specific symptoms, it is difficult to distinguish the two infections based on clinical manifestations. To identify and quantitatively detect Chikungunya and Dengue viruses, a real-time accelerated reverse-transcription-loop-mediated isothermal amplification (RT-LAMP) platform was developed, and 26-confirmed RNA samples, 42 suspects, and 18 healthy serum samples were evaluated by the method. The RT-polymerase chain reaction (PCR) and cDNA sequencing were used as references. The results showed that it could identify the Chikungunya and Dengue virus RNA correctly in all antibody-positive samples within 1 hour, without any cross-reactions. The virus load of the positive samples was quantitatively detected with a turbidimeter. The sensitivity was 100% and specificity was 95.25%. The findings indicate that the RT-LAMP is an effective method for rapid quantity detection of Chikungunya virus and Dengue virus in serum samples with convenient operation, high specificity, and high sensitivity.
authors.affiliation: Department of Respiratory, Tangdu Hospital, Fourth Military Medical University, Xi'an, China. xi.lu@mail.scut.edu.cn
authors.forename: Xi
authors.initials: X
authors.lastname: Lu
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Li
authors.forename: Ziyao
authors.initials: Z
authors.lastname: Mo
authors.forename: Faguang
authors.initials: F
authors.lastname: Jin
authors.forename: Boliang
authors.initials: B
authors.lastname: Wang
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhao
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Shan
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
mesh_terms: D001483:Base Sequence; D002646:Chikungunya virus / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004279:DNA, Viral / Q000302:isolation & purification; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004279:DNA, Viral