title: Anti-proliferative activity and apoptotic potential of britannin, a sesquiterpene lactone from Inula aucheriana.
abstract: Inula aucheriana n-hexane, CHCl3 and MeOH extracts were evaluated for their anti-proliferative activity against HepG-2, MCF-7, MDBK and A-549 cells. The CHCl3 extract exhibited cytotoxic activity to the above cell lines with IC50 values of 13.5, 23.4, 10.5, and 26.9 microg/mL, respectively. The sesquiterpene lactone britannin was isolated from the above extract. This was further evaluated in the MTT assay to demonstrate strong cytotoxicity to the mentioned cell lines (IC50: 2.2, 5.9, 5.4, and 3.5 microg/mL, respectively), and the apoptotic inducing properties of britannin were evaluated on human breast adenocarcinoma (MCF-7) cells through the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay.
authors.affiliation: Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam Hamzeloo
authors.initials: MH
authors.lastname: Moghadam
authors.forename: Homa
authors.initials: H
authors.lastname: Hajimehdipoor
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Saeidnia
authors.forename: Azadeh
authors.initials: A
authors.lastname: Atoofi
authors.forename: Roxana
authors.initials: R
authors.lastname: Shahrestani
authors.forename: Roger W
authors.initials: RW
authors.lastname: Read
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Mosaddegh
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D038143:Inula / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry* / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007783:Lactones; D012717:Sesquiterpenes; C575454:britannin