title: A re-assigned American mink (Neovison vison) map optimal for genome-wide studies.
abstract: Our previously published second generation genetic map for the American mink (Neovison vison) has been used and redesigned in its best for genome-wide studies with maximum of efficiency. A number of 114 selected markers, including 33 newly developed microsatellite markers from the CHORI-231 mink Bacterial Artificial Chromosome (BAC) library, have been genotyped in a two generation population composed of 1200 individuals. The outcome reassigns the position of some markers on the chromosomes and it produces a more reliable map with a convenient distance between markers. A total of 104 markers mapped to 14 linkage groups corresponding to the mink autosomes. Six markers are unlinked and four markers are allocated to the X chromosome by homology but no linkage was detected. The sex-average linkage map spans 1192 centiMorgans (cM) with an average intermarker distance of 11.4cM and 1648cM when the ends of the linkage groups and the autosomal unlinked markers are added. Sex-specific genetic linkage maps were also generated. The male sex-specific map had a total length of 1014.6cM between the linked markers and an average inter-marker interval of 9.7cM. The female map has a corresponding length of 1378.6cM and an average inter-marker interval of 13.3cM. The study is complemented with additional anchorage for most of the chromosomes of the map by BAC in situ hybridization with clones containing microsatellites strategically selected from the various parts of the genome. This map provides an improved tool for genetic mapping and comparative genomics in mink, also useful for the future assembly of the mink genome sequence when this will be taken forward.
authors.affiliation: University of Copenhagen, The Faculty of Life Sciences, Department of Basic Animaland Veterinary Sciences, Division of Animal Genetics and Bioinformatics, Groennegaardsvej 3, Frederiksberg C, Denmark. ran@life.ku.dk
authors.forename: Razvan
authors.initials: R
authors.lastname: Anistoroaei
authors.forename: Vivi
authors.initials: V
authors.lastname: Nielsen
authors.forename: Marios Nektarios
authors.initials: MN
authors.lastname: Markakis
authors.forename: Peter
authors.initials: P
authors.lastname: Karlskov-Mortensen
authors.forename: Claus B
authors.initials: CB
authors.lastname: Jørgensen
authors.forename: Knud
authors.initials: K
authors.lastname: Christensen
authors.forename: Merete
authors.initials: M
authors.lastname: Fredholm
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D004285:Dogs; D005260:Female; D016678:Genome; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D018895:Microsatellite Repeats; D008907:Mink / Q000235:genetics*; D013045:Species Specificity