title: Sickle cell mice exhibit mechanical allodynia and enhanced responsiveness in light touch cutaneous mechanoreceptors.
abstract: BACKGROUND
  Sickle cell disease (SCD) is associated with both acute vaso-occlusive painful events as well as chronic pain syndromes, including heightened sensitivity to touch. We have previously shown that mice with severe SCD (HbSS mice; express 100% human sickle hemoglobin in red blood cells; RBCs) have sensitized nociceptors, which contribute to increased mechanical sensitivity. Yet, the hypersensitivity in these neural populations alone may not fully explain the mechanical allodynia phenotype in mouse and humans.
  
  
  RESULTS
  Using the Light Touch Behavioral Assay, we found HbSS mice exhibited increased responses to repeated application of both innocuous punctate and dynamic force compared to control HbAA mice (100% normal human hemoglobin). HbSS mice exhibited a 2-fold increase in percent response to a 0.7mN von Frey monofilament when compared to control HbAA mice. Moreover, HbSS mice exhibited a 1.7-fold increase in percent response to the dynamic light touch "puffed" cotton swab stimulus. We further investigated the mechanisms that drive this behavioral phenotype by focusing on the cutaneous sensory neurons that primarily transduce innocuous, light touch. Low threshold cutaneous afferents from HbSS mice exhibited sensitization to mechanical stimuli that manifested as an increase in the number of evoked action potentials to suprathreshold force. Rapidly adapting (RA) Aβ and Aδ D-hair fibers showed the greatest sensitization, each with a 75% increase in suprathreshold firing compared to controls. Slowly adapting (SA) Aβ afferents had a 25% increase in suprathreshold firing compared to HbAA controls.
  
  
  CONCLUSIONS
  These novel findings demonstrate mice with severe SCD exhibit mechanical allodynia to both punctate and dynamic light touch and suggest that this behavioral phenotype may be mediated in part by the sensitization of light touch cutaneous afferent fibers to suprathreshold force. These findings indicate that Aβ fibers can be sensitized to mechanical force and should potentially be examined for sensitization in other tissue injury and disease models.
authors.affiliation: Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Sheldon R
authors.initials: SR
authors.lastname: Garrison
authors.forename: Audra A
authors.initials: AA
authors.lastname: Kramer
authors.forename: Nashaat Z
authors.initials: NZ
authors.lastname: Gerges
authors.forename: Cheryl A
authors.initials: CA
authors.lastname: Hillery
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Stucky
mesh_terms: D000200:Action Potentials; D000755:Anemia, Sickle Cell / Q000150:complications* / Q000473:pathology* / Q000503:physiopathology; D000818:Animals; D006801:Humans; D006930:Hyperalgesia / Q000150:complications* / Q000473:pathology* / Q000503:physiopathology; D008465:Mechanoreceptors / Q000378:metabolism*; D051379:Mice; D009043:Motor Activity; D009412:Nerve Fibers / Q000378:metabolism / Q000473:pathology; D009475:Neurons, Afferent / Q000378:metabolism / Q000473:pathology; D010812:Physical Stimulation; D012867:Skin / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D014110:Touch