title: Aspergillus bertholletius sp. nov. from Brazil nuts.
abstract: During a study on the mycobiota of brazil nuts (Bertholletia excelsa) in Brazil, a new Aspergillus species, A. bertholletius, was found, and is described here. A polyphasic approach was applied using morphological characters, extrolite data as well as partial β-tubulin, calmodulin and ITS sequences to characterize this taxon. A. bertholletius is represented by nineteen isolates from samples of brazil nuts at various stages of production and soil close to Bertholletia excelsa trees. The following extrolites were produced by this species: aflavinin, cyclopiazonic acid, kojic acid, tenuazonic acid and ustilaginoidin C. Phylogenetic analysis using partial β-tubulin and camodulin gene sequences showed that A. bertholletius represents a new phylogenetic clade in Aspergillus section Flavi. The type strain of A. bertholletius is CCT 7615 ( = ITAL 270/06 = IBT 29228).
authors.affiliation: Centro de Ciência e Qualidade de Alimentos, Instituto de Tecnologia de Alimentos, Campinas, São Paulo, Brazil. marta@ital.sp.gov.br
authors.forename: Marta H
authors.initials: MH
authors.lastname: Taniwaki
authors.forename: John I
authors.initials: JI
authors.lastname: Pitt
authors.forename: Beatriz T
authors.initials: BT
authors.lastname: Iamanaka
authors.forename: Daniele
authors.initials: D
authors.lastname: Sartori
authors.forename: Marina V
authors.initials: MV
authors.lastname: Copetti
authors.forename: Arun
authors.initials: A
authors.lastname: Balajee
authors.forename: Maria Helena P
authors.initials: MH
authors.lastname: Fungaro
authors.forename: Jens C
authors.initials: JC
authors.lastname: Frisvad
mesh_terms: D001230:Aspergillus / Q000235:genetics* / Q000502:physiology*; D031383:Bertholletia / Q000235:genetics / Q000382:microbiology*; D002147:Calmodulin / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004271:DNA, Fungal / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D008957:Models, Genetic; D009711:Nucleotides / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D014404:Tubulin / Q000378:metabolism
chemical_list: D002147:Calmodulin; D004271:DNA, Fungal; D021901:DNA, Intergenic; D009711:Nucleotides; D014404:Tubulin