title: Physicochemical characteristics and antioxidant activity of arginine-modified melanin from Lachnum YM-346.
abstract: Seven kinds of amino acids were used to modify the non-water-soluble extracellular melanin (LEM346) from Lachnum YM-346. It was found that arginine-melanin (ALEM346) had the highest solubility, being 4.55% (gg(-1)) in 30°C distilled water. Elemental analysis, infrared spectrum and mass spectrum analysis revealed that LEM346 molecule contained indole quinone structure, its molecular formula speculated to be C(18)H(8)O(6)N(2). Infrared spectrum analysis showed that ALEM346 had characteristic absorption peaks at 1672.346 and 1637.679 cm(-1). Mass spectrum analysis indicated that ALEM346 contained three types of arginine-melanin molecules. When the ALEM346 concentration was 500 mgL(-1), its total antioxidant capacity was equivalent to α-tocopherol of 46.65 mmolL(-1), the DPPH() and O(2)(-) scavenging rates and the Fe(2+) chelating rate were 89.05%, 93.81% and 80.18%, respectively, suggesting that the antioxidant activity of ALEM346 was stronger than that of LEM346.
authors.affiliation: College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China. yeming123@sina.com
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Geng-yi
authors.initials: GY
authors.lastname: Guo
authors.forename: Yun-long
authors.initials: YL
authors.lastname: He
authors.forename: Ying
authors.initials: Y
authors.lastname: Lu
authors.forename: Ying-wang
authors.initials: YW
authors.lastname: Ye
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: Yang
authors.forename: Pei-zhou
authors.initials: PZ
authors.lastname: Yang
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism; D001120:Arginine / Q000737:chemistry*; D001203:Ascomycota / Q000737:chemistry / Q000378:metabolism*; D055598:Chemical Phenomena; D008543:Melanins / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D012995:Solubility
chemical_list: D000975:Antioxidants; D008543:Melanins; D001120:Arginine