title: [Effects of resveratrol on the morphology of lipid droplets and the expression of lipid droplet-associated proteins in mouse primary hepatocytes].
abstract: OBJECTIVE
  To investigate the effects of resveratrol on the morphology of lipid droplet (LD) and the expression of lipid droplet-associated proteins in primary hepatocytes of mice.
  
  
  METHODS
  We isolated and cultured the primary hepatocytes of mice using collagenase perfusion. The primary hepatocytes were stimulated with 200 μmol/L oleic acid (OA) for 12 h, and then added with 0 (control), 20, 50, 100 μmol/L resveratrol, respectively. Another 12 h later, we utilized Bodipy 493/503 staining to observe the morphology and amount of intracellular lipid droplets under fluorescence microscope. The Folch method and triglyceride (TG) quantitative kit were used to extract the total intracellular lipids and determine the TG contents. Western blotting was applied to analyze the expression levels of perilipin, adipophilin and TIP-47.
  
  
  RESULTS
  Compared with the control, the size and amount of lipid droplets in primary hepatocytes were reduced after treated with different concentrations of resveratrol. The quantitative analysis showed that the intracellular TG contents decreased markedly in a dose-dependent manner, but the most significant decrease was in 50 μmol/L resveratrol group. Western blotting showed that resveratrol reduced the expressions of perilipin, adipophilin and TIP-47 in the primary hepatocytes, especially the perilipin.
  
  
  CONCLUSIONS
  Resveratrol could inhibit intracellular lipid accumulation, which is most significant in 50 μmol/L concentration. The inhibiting effect of resveratrol may be caused by mediating the expression levels of lipid droplet-associated proteins in primary hepatocytes of mice.
authors.affiliation: Department of Pathology and Pathophysiology, Fourth Military Medical University, Xian, China. wang_chao1987@126.com
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Le
authors.initials: L
authors.lastname: Li
authors.forename: Xiu-min
authors.initials: XM
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Pei-zhen
authors.initials: PZ
authors.lastname: Hu
authors.forename: Zeng-shan
authors.initials: ZS
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000032:analysis; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005234:Fatty Liver / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D000070778:Perilipin-1; D000070780:Perilipin-2; D010750:Phosphoproteins / Q000032:analysis; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*; D014280:Triglycerides / Q000032:analysis
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D000070778:Perilipin-1; D000070780:Perilipin-2; D010750:Phosphoproteins; C000602983:Plin2 protein, mouse; D013267:Stilbenes; D014280:Triglycerides; D000077185:Resveratrol