title: Acute and Subchronic Inhalation Toxicity of n-Octane in Rats.
abstract: OBJECTIVE
  We have investigated the toxic effects of the inhalation of subchronic and acute levels of n-octane.
  
  
  METHODS
  The rats were exposed to n-octane of 0, 2.34, 11.68 and 23.36 mg/L (n = 5 rats/group/gender) in an acute inhalation test (Organization for Economic Co-operation and Development (OECD) TG 403), or to 0, 0.93, 2.62 and 7.48 mg/L (n = 10 rats/group/gender) for a subchronic inhalation test (OECE TG 413), to establish a national chemical management system consistent with the Globally Harmonized Classification System (GHS).
  
  
  RESULTS
  Acutely-exposed rats became lethargic but recovered following discontinuation of inhalation. Other clinical symptoms such as change of body weight and autopsy finds were absent. The LC50 for the acute inhalation toxicity of n-octane was determined to exceed 23.36 mg/L and the GHS category was 'not grouping'. Subchronically-treated rats displayed no significant clinical and histopathological differences from untreated controls; also, target organs were affected hematologically, biochemically and pathologically. Therefore, the no observable adverse effect level was indicated as exceeding 7.48 mg/L and the GHS category was 'not grouping' for the specific target organ toxicity upon repeated exposure.
  
  
  CONCLUSIONS
  However, n-octane exposure should be controlled to be below the American Conference of Industrial Hygienists recommendation (300 ppm) to prevent inhalation-related adverse health effects of workers.
authors.affiliation: Toxicity Assessment Department, Korea Environment and Merchandise Testing Institute, Incheon, Korea.
authors.forename: Jae Hyuck
authors.initials: JH
authors.lastname: Sung
authors.forename: Byung-Gil
authors.initials: BG
authors.lastname: Choi
authors.forename: Hyeon Yeong
authors.initials: HY
authors.lastname: Kim
authors.forename: Min-Won
authors.initials: MW
authors.lastname: Baek
authors.forename: Hyun Youl
authors.initials: HY
authors.lastname: Ryu
authors.forename: Yong Soon
authors.initials: YS
authors.lastname: Kim
authors.forename: Young Kuk
authors.initials: YK
authors.lastname: Choi
authors.forename: Il Je
authors.initials: IJ
authors.lastname: Yu
authors.forename: Kyung Seuk
authors.initials: KS
authors.lastname: Song