title: The application of biochemical responses to assess environmental quality of tropical estuaries: field surveys.
abstract: A battery of biomarkers of exposure (EROD, DBF, GST and GPx) and effect (lipid peroxidation and DNA damage - strand breaks) were analyzed in gill tissues from caged and native oysters Crassostrea rhizophorae exposed to two tropical estuarine systems in SW Brazil: Santos (S1, S2, S3, S4) and Paranaguá (P1 - control, P2, P3, P4). The exposure lasted 28 days. Native oysters were sampled in the same areas where caged systems were exposed. Significant induction of biomarkers of exposure to organic compounds and oxidative stress (p < 0.05) were observed in all transplanted individuals from Santos resulting in DNA damage and lipid peroxidation. Biological adverse effects were more evident in oysters transplanted in the Santos Estuarine System, a recognized contaminated area, than in the Paranaguá Estuarine System, surrounded by urban areas and Environmental Protected Areas. Native specimens from both estuaries showed adaption to the impacts of several contamination sources affecting the ecosystem. The use of transplanted C. rhizophorae proved to be a suitable tool for assessing and monitoring the environmental quality in mangrove ecosystems. This integrated approach employing multi-biomarker responses under field conditions could be incorporated as a descriptor of health status in tropical estuarine systems.
authors.affiliation: Dep. Química Física, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz (UCA-CSIC), Campus Rio San Pedro s/n, 11510, Puerto Real, Cádiz, España. luciane.alves@uca.es
authors.forename: Luciane Alves
authors.initials: LA
authors.lastname: Maranho
authors.forename: Camilo Dias Seabra
authors.initials: CD
authors.lastname: Pereira
authors.forename: Rodrigo Brasil
authors.initials: RB
authors.lastname: Choueri
authors.forename: Augusto
authors.initials: A
authors.lastname: Cesar
authors.forename: Paloma Kachel
authors.initials: PK
authors.lastname: Gusso-Choueri
authors.forename: Ronaldo José
authors.initials: RJ
authors.lastname: Torres
authors.forename: Denis Moledo de Souza
authors.initials: DM
authors.lastname: Abessa
authors.forename: Rodofley Davino
authors.initials: RD
authors.lastname: Morais
authors.forename: Antônio Aparecido
authors.initials: AA
authors.lastname: Mozeto
authors.forename: Tomás Angel
authors.initials: TA
authors.lastname: DelValls
authors.forename: María Laura
authors.initials: ML
authors.lastname: Martín-Díaz
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001938:Brazil; D049896:Crassostrea / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods*; D063366:Estuaries; D005452:Fluoresceins / Q000378:metabolism; D005880:Gills / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D015415:Biomarkers; D005452:Fluoresceins; D014874:Water Pollutants, Chemical; C558664:dibenzylfluorescein; D005979:Glutathione Peroxidase; D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase