title: Structure of hepatitis C virus envelope glycoprotein E2 antigenic site 412 to 423 in complex with antibody AP33.
abstract: We have determined the crystal structure of the broadly neutralizing antibody (bnAb) AP33, bound to a peptide corresponding to hepatitis C virus (HCV) E2 envelope glycoprotein antigenic site 412 to 423. Comparison with bnAb HCV1 bound to the same epitope reveals a different angle of approach to the antigen by bnAb AP33 and slight variation in its β-hairpin conformation of the epitope. These structures establish two different modes of binding to E2 that antibodies adopt to neutralize diverse HCV.
authors.affiliation: Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Leopold
authors.initials: L
authors.lastname: Kong
authors.forename: Erick
authors.initials: E
authors.lastname: Giang
authors.forename: Travis
authors.initials: T
authors.lastname: Nieusma
authors.forename: Justin B
authors.initials: JB
authors.lastname: Robbins
authors.forename: Marc C
authors.initials: MC
authors.lastname: Deller
authors.forename: Robyn L
authors.initials: RL
authors.lastname: Stanfield
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
authors.forename: Mansun
authors.initials: M
authors.lastname: Law
mesh_terms: D057134:Antibodies, Neutralizing / Q000737:chemistry* / Q000378:metabolism; D000939:Epitopes / Q000737:chemistry / Q000235:genetics; D016174:Hepacivirus / Q000235:genetics*; D008958:Models, Molecular; D011487:Protein Conformation; D014759:Viral Envelope Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D057134:Antibodies, Neutralizing; D000939:Epitopes; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus