title: Antifibrotic activity of diarylheptanoids from Betula platyphylla toward HSC-T6 cells.
abstract: A chemical investigation of the n-butanol fraction of the inner bark of Betula platyphylla led to the isolation of seven diarylhepanoids, (-)-centrolobol (1), aceroside VII (2), aceroside VIII (3), (3R)-1,7-bis-(4-hydroxyphenyl)-3-heptanol-3-O-[2,6-bis-O-(β-D-apiofuranosyl)-β-D-glucopyranoside (4), 1,7-bis-(4-hydroxyphenyl)-5-hepten-3-one (5), platyphyllone (6) and platyphylloside (7). The antifibrotic effects of these isolates were evaluated with HSC-T6 cells by assessing cell proliferation. Among them, compounds 1, 2, 5 and 6 significantly inhibited the proliferation of HSCs in a dose-dependent manner at concentrations from 10 µM to 100 µM. Compound 5 in particular dramatically decreased the collagen content and increased the Caspase-3/7 activity. Taken together, the antifibrotic activity of B. platyphylla and its constituents might suggest therapeutic potential against liver fibrosis.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, San 56-1, Sillim-Dong, Seoul 151-742, Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Lee
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Park
authors.forename: Dong Sun
authors.initials: DS
authors.lastname: Min
authors.forename: Hunseung
authors.initials: H
authors.lastname: Yoo
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Young Choong
authors.initials: YC
authors.lastname: Kim
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
mesh_terms: D000818:Animals; D029662:Betula / Q000737:chemistry*; D015415:Biomarkers / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053179:Caspase 7 / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D036381:Diarylheptanoids / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects; D055166:Hepatic Stellate Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats
chemical_list: D015415:Biomarkers; D036381:Diarylheptanoids; D010936:Plant Extracts; D003094:Collagen; D053148:Caspase 3; D053179:Caspase 7