title: Effects of 28 days silicon dioxide aerosol exposure on respiratory parameters, blood biochemical variables and lung histopathology in rats.
abstract: Inhalation toxicity of silicon dioxide aerosol (150, 300 mg/m(3)) daily over a period of 28 days was carried out in rats. The changes in respiratory variables during the period of exposure were monitored using a computer programme that recognizes the modifications of the breathing pattern. Exposure to the aerosol caused a time dependent decrease in tidal volume, with an increase in respiratory frequency compared to the control. Biochemical variables and histopathological observation were noted at 28th day following the start of exposure. Biochemical markers of silica induced lung injury like plasma alkaline phosphatase, lactate dehydrogenase and angiotensine converting enzyme activities increased in a concentration dependent manner compared to control. Increase in the plasma enzymatic activities indicates endothelial lung damage, increased lung membrane permeability. Histopathological observation of the lungs confirmed concentration dependent granulomatous inflammation, fibrosis and proteinacious degeneration. Aggregates of mononuclear cells with entrapped silica particles circumscribed by fibroblast were observed in 300 mg/m(3) silica aerosol exposed group at higher magnification. Decrease in tidal volume and increase in respiratory frequency might be due to the thickening of the alveolar wall leading to a decreased alveolar volume and lowered elasticity of the lung tissue. The trends in histological and biochemical data are in conformity with the respiratory data in the present study. This study reports for the first time, the changes in respiratory variables during silica aerosol exposure over a period of 28 days.
authors.affiliation: Division of Pharmacology and Toxicology, Defence Research & Development Establishment, Gwalior, India. utsab.deb@rediffmail.com
authors.forename: Utsab
authors.initials: U
authors.lastname: Deb
authors.forename: Vinay
authors.initials: V
authors.lastname: Lomash
authors.forename: Suchita
authors.initials: S
authors.lastname: Raghuvanshi
authors.forename: S C
authors.initials: SC
authors.lastname: Pant
authors.forename: R
authors.initials: R
authors.lastname: Vijayaraghavan
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000336:Aerosols / Q000633:toxicity*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D019570:Inhalation Exposure / Q000009:adverse effects; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D011650:Pulmonary Alveoli; D051381:Rats; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000633:toxicity*; D013990:Tidal Volume
chemical_list: D000336:Aerosols; D015415:Biomarkers; D012822:Silicon Dioxide; D007770:L-Lactate Dehydrogenase; D000110:Acetylcholinesterase