title: Comparative FTIR study of a new fungal rhodopsin.
abstract: Bacteriorhodopsin (BR) is a light-driven proton pump of halophilic Archaea , and BR-like proton-pumping rhodopsins have been discovered in Bacteria and Eucarya as well. Leptosphaeria rhodopsin (LR) and Phaeosphaeria Rhodopsin 2 (PhaeoRD2) are both fungal rhodopsins in such a functional class, even though they belong to different branches of the phylogenetic tree. In this study, we compared light-induced structural changes in the K, L, and M photointermediates for PhaeoRD2, LR, and BR using low-temperature Fourier transform infrared (FTIR) spectroscopy. We observed a strongly hydrogen-bonded water molecule in PhaeoRD2 (water O-D stretch in D(2)O at 2258 cm(-1)) as well as in LR and BR. This observation provided additional experimental evidence to the concept that strongly hydrogen-bonded water molecule is the functional determinant of light-driven proton pumping. The difference FTIR spectra for all the K, L, and M states are surprisingly similar between PhaeoRD2 and LR, but not for BR. PhaeoRD2 is more homologous to LR than to BR, but the difference is small. The amino acid identities between PhaeoRD2 and LR, and between PhaeoRD2 and BR are 34.5% and 30.2%, respectively. In addition, the amino acids uniquely identical for the fungal rhodopsins are located rather far from the retinal chromophore. In fact, the amino acid identities within 4 Å from retinal are the same among PhaeoRD2, LR, and BR. For more than 100 amino acids located within 12 Å from retinal, the identities are 48.7% between PhaeoRD2 and LR, 46.0% between PhaeoRD2 and BR, and 47.8% between LR and BR. These results suggest that protein core structures are equally different among the three rhodopsins. Thus, the identical FTIR spectra between PhaeoRD2 and LR (but not BR), even for the K state, indicate that fungal rhodopsins possess some common structural motif and dynamics not obvious from the amino acid sequences.
authors.affiliation: Department of Frontier Materials, Nagoya Institute of Technology, Nagoya 466-8555, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Ito
authors.forename: Masayo
authors.initials: M
authors.lastname: Sumii
authors.forename: Akira
authors.initials: A
authors.lastname: Kawanabe
authors.forename: Ying
authors.initials: Y
authors.lastname: Fan
authors.forename: Yuji
authors.initials: Y
authors.lastname: Furutani
authors.forename: Leonid S
authors.initials: LS
authors.lastname: Brown
authors.forename: Hideki
authors.initials: H
authors.lastname: Kandori
mesh_terms: D001203:Ascomycota; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism*; D007919:Leptospira; D008958:Models, Molecular; D011487:Protein Conformation; D012243:Rhodopsin / Q000737:chemistry* / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D012243:Rhodopsin