title: A one step multiplex PCR assay for rapid screening of East Asian mtDNA haplogroups on forensic samples.
abstract: The mitochondrial DNA (mtDNA) haplogroup typing has become an essential tool to study human evolutionary history and to infer the matrilineal bio-geographic ancestry. In forensic field, the screening of mtDNA haplogroups by genotyping of mtDNA single nucleotide polymorphisms (SNPs) can help guarantee the quality of mtDNA sequence data as well as can reduce the need to sequence samples that do not match. Here, a multiplex mutagenically separated (MS) polymerase chain reaction (PCR) system was developed for simultaneous rapid detection of 14 coding region SNPs and one deletion motif representing common mtDNA haplogroups of East Asia. The multiplex MS PCR system we developed has the advantage of being a one step procedure that requires only a single PCR amplification with allele-specific primers and allowing straightforward designation of haplogroups along the branches of the phylogenetic tree. Therefore, it would be a simple, rapid, and reliable detection method useful for large-scale screening of mtDNA variations to determine East Asian mtDNA haplogroups.
authors.affiliation: Department of Forensic Medicine, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, South Korea.
authors.forename: Hwan Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Jung Ah
authors.initials: JA
authors.lastname: Yoon
authors.forename: Woo Ick
authors.initials: WI
authors.lastname: Yang
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Shin
mesh_terms: D044466:Asian People / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D004272:DNA, Mitochondrial / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods*; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D056910:Republic of Korea
chemical_list: D004272:DNA, Mitochondrial