title: Mutual enhancement of the current density and the coulombic efficiency for a bioanode by entrapping bi-enzymes with Os-complex modified electrodeposition paints.
abstract: A bioanode with high current density and coulombic efficiency was developed by co-immobilization of pyranose dehydrogenase from Agaricus meleagris (AmPDH) with the dehydrogenase domain of cellobiose dehydrogenase from Corynascus thermophiles (recDHCtCDH) expressed recombinantly in Escherichia coli. The two enzymes were entrapped in Os-complex modified electrodeposition polymers (Os-EDPs) with specifically adapted redox potential by means of chemical co-deposition. AmPDH oxidizes glucose at both the C2 and C3 positions whereas recDHCtCDH oxidizes glucose only at the C1 position. Electrochemical measurements reveal that maximally 6 electrons can be harvested from one glucose molecule at the two-enzyme anode via a cascade reaction, as AmPDH oxidizes the products formed from of the recDHCtCDH catalyzed substrate oxidation and vice versa. Furthermore, a significant increase in current density can be obtained by combining AmPDH and recDHCtCDH in a single modified electrode. We propose the use of this bioanode in biofuel cells with increased current density and coulombic efficiency.
authors.affiliation: Analytische Chemie-Elektroanalytik & Sensorik, Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
authors.forename: Minling
authors.initials: M
authors.lastname: Shao
authors.forename: Muhammad Nadeem
authors.initials: MN
authors.lastname: Zafar
authors.forename: Christoph
authors.initials: C
authors.lastname: Sygmund
authors.forename: Dmitrii A
authors.initials: DA
authors.lastname: Guschin
authors.forename: Roland
authors.initials: R
authors.lastname: Ludwig
authors.forename: Clemens K
authors.initials: CK
authors.lastname: Peterbauer
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schuhmann
authors.forename: Lo
authors.initials: L
authors.lastname: Gorton
mesh_terms: D000364:Agaricus / Q000201:enzymology* / Q000235:genetics; D001203:Ascomycota / Q000201:enzymology*; D001674:Bioelectric Energy Sources; D004566:Electrodes; D004595:Electroplating / Q000379:methods*; D004735:Energy Transfer; D004867:Equipment Design; D019544:Equipment Failure Analysis; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry
chemical_list: D011993:Recombinant Fusion Proteins; D010088:Oxidoreductases