title: Application of immunomagnetic particles to enzyme-linked immunosorbent assay (ELISA) for improvement of detection sensitivity of HCG.
abstract: This investigation was aimed at using superparamagnetic particles to enzyme-linked immunosorbent assay (SPIO-ELISA) of human chorionic gonadotropin (hCG) to enhance detection sensitivity of hCG. We found that N-(3-dimethyl aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) was the best cross-linking reagent to link anti hCG α antibody to superparamagnetic particle (SPIO-anti hCG α antibody immunomagnetic particle). To improve the specificity of the assay, a horse radish peroxidase (HRP)-labeled anti-hCG beta monoclonal antibody was used to detect captured hCG using double antibody sandwich ELISA assay. SPIO-ELISA application to determine hCG increased the sensitivity to 1 mIU/mL, which is a level of sensitivity enabling the diagnosis of pregnancy during the early gestational period.
authors.affiliation: Department of Food Science, National Pingtung University of Science and Technology, Taiwan.
authors.forename: Hsiao-Ting
authors.initials: HT
authors.lastname: Kuo
authors.forename: Jay Z
authors.initials: JZ
authors.lastname: Yeh
authors.forename: Po-Hua
authors.initials: PH
authors.lastname: Wu
authors.forename: Chii-Ming
authors.initials: CM
authors.lastname: Jiang
authors.forename: Ming-Chang
authors.initials: MC
authors.lastname: Wu
mesh_terms: D000906:Antibodies / Q000737:chemistry; D006063:Chorionic Gonadotropin / Q000032:analysis*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D000906:Antibodies; D006063:Chorionic Gonadotropin; D003432:Cross-Linking Reagents; D058185:Magnetite Nanoparticles