title: Quantitative approach for small molecules using laser desorption/ionization mass spectrometry.
abstract: Recent advances in quantitative approaches to laser desorption/ionization mass spectrometry (LDI-MS) are selectively reviewed. Numerous efforts have been made to use matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and related techniques for rapid screening and/or monitoring of biological events. Among the various mass ionization spectrometric analysis techniques, MALDI-MS has exceptional potential for high-throughput analysis. Although quantitative analysis with MALDI-MS is challenging, the expansion of its utility is inevitable. It is notable that practical improvement of MALDI-MS can be achieved by preparation of an uniform matrix using binary matrix systems. Broad applications have been established with a self-assembled monolayer for MALDI-MS, referred to as SAMDI-MS. The method could be an essential tool not only for rapid screening but also as a sensitive probe for surface sciences. Complementary to these approaches, labeling of molecules for LDI-MS is introduced as potential tool for the selective detection of specific target molecules.
authors.affiliation: Center for Industry, University and Government Cooperation, Nagasaki University, Nagasaki, Japan. maki@nagasaki-u.ac.jp
authors.forename: Toshihide
authors.initials: T
authors.lastname: Maki
mesh_terms: D001671:Biochemistry / Q000379:methods*; D007834:Lasers; D008970:Molecular Weight; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*