title: Upregulation of vascular endothelial growth factor receptor-3 in the spinal cord of Lewis rats with experimental autoimmune encephalomyelitis.
abstract: We investigated the spatiotemporal expression of vascular endothelial growth factor receptor-3 (VEGFR-3) in the spinal cord of Lewis rats with experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis. VEGFR-3 mRNA and protein were constitutively expressed in gray matter neurons and in a few white matter astrocytes. Induction of VEGFR-3 occurred predominantly in perivascular infiltrated macrophages in the spinal cord white matter during the inductive phase of EAE. VEGFR-3 expression was also induced in activated microglial cells in the gray and white matter, mainly in the peak phase. In addition, reactive astrocytes in the white matter, but not in the gray matter, expressed VEGFR-3 as disease severity increased. These data suggest that VEGFR-3 is involved in the recruitment of monocytic macrophages and in glial reactions during EAE.
authors.affiliation: Department of Anatomy, The Catholic University of Korea, Seoul, Korea.
authors.forename: Jang-Mi
authors.initials: JM
authors.lastname: Park
authors.forename: Yoo-Jin
authors.initials: YJ
authors.lastname: Shin
authors.forename: Jeong Min
authors.initials: JM
authors.lastname: Cho
authors.forename: Jae-Youn
authors.initials: JY
authors.lastname: Choi
authors.forename: Sin-Soo
authors.initials: SS
authors.lastname: Jeun
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Cha
authors.forename: Mun-Yong
authors.initials: MY
authors.lastname: Lee
mesh_terms: D000208:Acute Disease; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D005260:Female; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008264:Macrophages / Q000378:metabolism; D017628:Microglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011917:Rats, Inbred Lew; D060888:Real-Time Polymerase Chain Reaction; D013116:Spinal Cord / Q000378:metabolism*; D015854:Up-Regulation; D042582:Vascular Endothelial Growth Factor C / Q000096:biosynthesis / Q000235:genetics; D040321:Vascular Endothelial Growth Factor Receptor-3 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D042582:Vascular Endothelial Growth Factor C; D040321:Vascular Endothelial Growth Factor Receptor-3