title: The progesterone-responsive gene 14-3-3τ enhances the transcriptional activity of progesterone receptor in uterine cells.
abstract: Members of the 14-3-3 family are intracellular dimeric phosphoserine-binding proteins that can associate with and modulate the activities of many proteins. In our efforts to isolate the genes regulated by progesterone (P(4)) using suppressive subtractive hybridization, we previously found that 14-3-3τ is one of the genes upregulated by P(4). In this study, we demonstrated by quantitative RT-PCR (qRT-PCR), western blot analyses, and immunohistochemistry that 14-3-3τ mRNA and protein levels were increased in the rat uterus after P(4) treatment. Furthermore, qRT-PCR indicated that P(4) increased 14-3-3τ mRNA levels in human endometrial epithelial cells and endometrial stromal cells (ESCs). Western blot and qRT-PCR analyses revealed that in vitro decidualization using cAMP and medroxyprogesterone 17-acetate increased levels of 14-3-3τ mRNA and protein in ESCs. We have shown by qRT-PCR and western blot analyses that P(4) increased the mRNA and protein levels of 14-3-3τ in Ishikawa cells that stably express P(4) receptor-B (PR-B). Immunocytochemistry revealed that 14-3-3τ colocalizes with PR and translocates from the cytoplasm to the nucleus in response to P(4). Moreover, by luciferase reporter assay, we demonstrated that 14-3-3τ enhances the transcriptional activity of PR-B. Taken together, we propose that 14-3-3τ is a P(4)-responsive gene in uterine cells that modulates P(4) signaling.
authors.affiliation: Department of Geriatric Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Ito
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Urano
authors.forename: Hisahiko
authors.initials: H
authors.lastname: Hiroi
authors.forename: Mikio
authors.initials: M
authors.lastname: Momoeda
authors.forename: Mayuko
authors.initials: M
authors.lastname: Saito
authors.forename: Yumi
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Tsutsumi
authors.forename: Fumiko
authors.initials: F
authors.lastname: Zenri
authors.forename: Minako
authors.initials: M
authors.lastname: Koizumi
authors.forename: Hanako
authors.initials: H
authors.lastname: Nakae
authors.forename: Kuniko
authors.initials: K
authors.lastname: Horie-Inoue
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fujii
authors.forename: Tetsu
authors.initials: T
authors.lastname: Yano
authors.forename: Shiro
authors.initials: S
authors.lastname: Kozuma
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inoue
authors.forename: Yuji
authors.initials: Y
authors.lastname: Taketani
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D047468:Immunoprecipitation; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011980:Receptors, Progesterone / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014599:Uterus / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*
chemical_list: D048948:14-3-3 Proteins; D011980:Receptors, Progesterone; D011374:Progesterone; D017258:Medroxyprogesterone Acetate; D000242:Cyclic AMP