title: [Genetic characteristics of EV71 in Yunnan Province from 2009 to 2010].
abstract: In order to learn about the genetic characteristic of human enterovirus type71 (HEV71) isolated from cases of Hand, Foot and Mouth Disease (HFMD) in Yunnan Province from 2009 to 2010. 50 isolates form HFMD cases were performed entire VP1 coding region amplification by reverse transcription-polymerase chain reaction and sequencing the nucleotide sequences; then the phylogenetic tree was constructed. The complete nucleotide sequences of region VP1 of the 50 strains were all 891nt length coding 297 amino acids. The result of molecular identification of the 50 strains is HEV71. Phylogenetic analysis indicated that 48 EV71 isolates belonged to subgenotype C4a and 2 EV71 isolates belonged to genotype A. From 2009 to 2010, the pathogen of HFMD cases were EV71 strains in Yunnan province, which were co-evolved with isolates from other provinces in mainland of China. There was no significant difference found in the whole sequence of VP1 gene of the strains isolated from different regions or under different diseases occurred, but the spread of genetype A appared in Yunnan Province in 2009.
authors.affiliation: Kunming Medical University, Kunming 650031, China. liuguojun2010@126.com
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Bing-Jun
authors.initials: BJ
authors.lastname: Tian
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Cun
authors.forename: Jie
authors.initials: J
authors.lastname: Yin
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Jiang
authors.forename: Yue
authors.initials: Y
authors.lastname: Ma
authors.forename: Lin
authors.initials: L
authors.lastname: Lu
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
mesh_terms: D036022:Capsid Proteins / Q000235:genetics; D002675:Child, Preschool; D002681:China; D029821:Enterovirus A, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D005260:Female; D060005:Genotyping Techniques; D006232:Hand, Foot and Mouth Disease / Q000821:virology; D006801:Humans; D008297:Male; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D036022:Capsid Proteins; C442501:VP1 protein, Foot-and-mouth disease virus