title: Potent melanin production enhancement of human tyrosinase gene by Tat and an entrapment in elastic cationic niosomes: potential application in vitiligo gene therapy.
abstract: Potent melanin production enhancement of human tyrosinase plasmid (pAH7/Tyr, P) in mouse melanoma cells (B(16)F(10)) by Tat peptide (T) and an entrapment in elastic cationic niosomes (E) was described. The E composed of Tween 61/cholesterol/dodecyl dimethyl ammonium bromide at 1:1:0.5 molar ratio was prepared by freeze-dried emptying liposomes method. PE at P/E ratio of 1:160 w/w and TPE at T/P/E ratio of 0.125:1:160, 0.25:1:160, and 0.5:1:160 w/w/w were prepared. The final concentration of the plasmid in the study was 4 ng/μL. By sulforhodamine B assay, PE and TPE complexes showed slight or no cytotoxic effect. The cells transfected with TPE (0.5:1:160) exhibited the highest enhancement of tyrosinase enzyme activity of 11.82-, 7.67-, 5.07-, and 6.29-folds of control, P, PE, and TP (0.5:1) and melanin production of 13.03-, 8.46-, 5.36-, and 6.58-folds of control, P, PE, and TP (0.5:1), respectively. The elastic cationic niosomes demonstrated an increase in thermal stability of P at 4 ± 2, 25 ± 2, and 45 ± 2 °C. The vesicular size and the zeta potential values of PE and TPE complexes were slightly increased but still in the range of stable dispersion (out of ±30 mV). These results indicated the high potential application of the TPE complexes for further investigation for vitiligo gene therapy.
authors.affiliation: Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. jiradej.manosroi8@gmail.com
authors.forename: Jiradej
authors.initials: J
authors.lastname: Manosroi
authors.forename: Narinthorn
authors.initials: N
authors.lastname: Khositsuntiwong
authors.forename: Friedrich
authors.initials: F
authors.lastname: Götz
authors.forename: Rolf G
authors.initials: RG
authors.lastname: Werner
authors.forename: Worapaka
authors.initials: W
authors.lastname: Manosroi
authors.forename: Aranya
authors.initials: A
authors.lastname: Manosroi
mesh_terms: D000818:Animals; D001965:Bromides / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000737:chemistry; D005612:Freeze Drying; D015696:Gene Products, tat / Q000737:chemistry* / Q000378:metabolism; D015316:Genetic Therapy; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008543:Melanins / Q000378:metabolism*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics* / Q000378:metabolism; D010316:Particle Size; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011136:Polysorbates / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D014820:Vitiligo / Q000628:therapy*
chemical_list: D001965:Bromides; D002412:Cations; D015696:Gene Products, tat; D008081:Liposomes; D008543:Melanins; D011136:Polysorbates; D000644:Quaternary Ammonium Compounds; D002784:Cholesterol; D014442:Monophenol Monooxygenase; C051470:ammonium bromide