title: Magnetic transfer contrast accurately localizes substantia nigra confirmed by histology.
abstract: BACKGROUND
  Magnetic resonance imaging (MRI) has multiple contrast mechanisms. Like various staining techniques in histology, each contrast type reveals different information about the structure of the brain. However, it is not always clear how structures visible in MRI correspond to structures previously identified by histology. The purpose of this study was to determine if magnetic transfer contrast (MTC) or T2 contrast MRI was better at delineating the substantia nigra (SN).
  
  
  METHODS
  MRI scans were acquired in vivo from two nonhuman primates (NHPs). The NHPs were subsequently euthanized, perfused, and their brains sectioned for histologic analyses. Each slice was photographed before sectioning. Each brain was sectioned into approximately 500 sections, 40 μm each, encompassing most of the cortex, midbrain, and dorsal parts of the hindbrain. Levels corresponding to anatomic MRI images were selected. From these, adjacent sections were stained using Kluver-Barrera (myelin and cell bodies) or tyrosine hydroxylase (dopaminergic neurons) immunohistochemistry. The resulting images were coregistered to the block-face images using a moving least squares algorithm with similarity transformations. MR images were similarly coregistered to the block-face images, allowing the structures on MRI to be identified with structures on the histologic images.
  
  
  RESULTS
  We found that hyperintense (light) areas in MTC images were coextensive with the SN as delineated histologically. The hypointense (dark) areas in T2-weighted images were not coextensive with the SN but extended partially into the SN and partially into the cerebral peduncles.
  
  
  CONCLUSIONS
  MTC is more accurate than T2-weighting for localizing the SN in vivo.
authors.affiliation: Department of Vision Sciences, University of Alabama at Birmingham, Birmingham, AL, USA.
authors.forename: Mark S
authors.initials: MS
authors.lastname: Bolding
authors.forename: Meredith A
authors.initials: MA
authors.lastname: Reid
authors.forename: Kathy B
authors.initials: KB
authors.lastname: Avsar
authors.forename: Rosalinda C
authors.initials: RC
authors.lastname: Roberts
authors.forename: Paul D
authors.initials: PD
authors.lastname: Gamlin
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Gawne
authors.forename: David M
authors.initials: DM
authors.lastname: White
authors.forename: Jan A
authors.initials: JA
authors.lastname: den Hollander
authors.forename: Adrienne C
authors.initials: AC
authors.lastname: Lahti
mesh_terms: D000818:Animals; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008279:Magnetic Resonance Imaging; D008297:Male; D013378:Substantia Nigra / Q000033:anatomy & histology*