title: Development of a monoclonal antibody-based sandwich-type enzyme-linked immunosorbent assay (ELISA) for detection of abrin in food samples.
abstract: Abrin is a plant toxin, which can be easily isolated from the seeds of Abrus precatorius. It may be used as a biological warfare agent. In order to detect abrin in food samples, a two-layer sandwich format enzyme-linked immunosorbent assay based on the monoclonal antibody (mAb) (as capture antibody) and rabbit polyclonal serum (as detecting antibody) was developed and applied for the determination of abrin in some food matrices. The linear range of the mAb was 1-100 μg L(-1) with a detection limit of 0.5 μg L(-1) for abrin in phosphate buffered saline (PBS). The recoveries of abrin from sausage, beer and milk samples ranged 97.5-98.6%, 95.8-98.4% and 94.8-9.6%, respectively, with a coefficient of variation (CV) of 3.7% or less. The newly developed sandwich ELISA using the mAb appears to be a reliable and useful method for detection of abrin in sausage, beer and milk.
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China. zhouyu69@sina.com
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Xiang-Li
authors.initials: XL
authors.lastname: Tian
authors.forename: Yan-Song
authors.initials: YS
authors.lastname: Li
authors.forename: Feng-Guang
authors.initials: FG
authors.lastname: Pan
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Jun-Hui
authors.initials: JH
authors.lastname: Zhang
authors.forename: Xin-Rui
authors.initials: XR
authors.lastname: Wang
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Ren
authors.forename: Shi-Ying
authors.initials: SY
authors.lastname: Lu
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Li
authors.forename: Zeng-Shan
authors.initials: ZS
authors.lastname: Liu
authors.forename: Qi-Jun
authors.initials: QJ
authors.lastname: Chen
authors.forename: Jing-Qiu
authors.initials: JQ
authors.lastname: Liu
mesh_terms: D000036:Abrin / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D001515:Beer / Q000032:analysis*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D011817:Rabbits; D014118:Toxins, Biological / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D014118:Toxins, Biological; D000036:Abrin