title: Preparation of biomimetic hydrogels with controlled cell adhesive properties and topographical features for the study of muscle cell adhesion and proliferation.
abstract: Synthetic substrates with defined chemical and structural characteristics may potentially be prepared to mimic the living ECM to regulate cell adhesion and growth. Hydrogels with cell-adhesive peptides (0.28 ± 0.03 nmol peptide cm(-2) , TTA-R-0.5; and 0.91 ± 0.12 nmol peptide cm(-2) , TTA-R-2.0) and/or micro-scaled topographical patterns (10, 25, and 80 µm grooves) are prepared using enzymatic polymerization. The adherent morphology and proliferation of C2C12 skeletal myoblasts and human aortic smooth muscle cells (hAoSM) on the hydrogels are studied. The newly developed hydrogels may be useful in investigating the roles of cell adhesion and substrate surface properties in the communication of adherent cells with the ECM.
authors.affiliation: Department of Bioengineering, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Indong
authors.initials: I
authors.lastname: Jun
authors.forename: Seok Joo
authors.initials: SJ
authors.lastname: Kim
authors.forename: Eunpyo
authors.initials: E
authors.lastname: Choi
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Park
authors.forename: Taiyoun
authors.initials: T
authors.lastname: Rhim
authors.forename: Jungyul
authors.initials: J
authors.lastname: Park
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Park
authors.forename: Heungsoo
authors.initials: H
authors.lastname: Shin
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry; D006801:Humans; D020100:Hydrogels; D008422:Materials Testing; D051379:Mice; D008855:Microscopy, Electron, Scanning; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000187:drug effects*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects*; D010455:Peptides / Q000138:chemical synthesis*; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D004129:Dimethylpolysiloxanes; D020100:Hydrogels; D010455:Peptides; C013830:baysilon