title: Sperm from pheromone primed brown trout (Salmo trutta L.) produce more larvae.
abstract: Male goldfish (Carassius auratus) exposed to female hormonal pheromones express increased milt volumes and their sperm fertilize more eggs than sperm from unprimed males. Ovulated salmonid females also release odours that increase volumes of strippable milt in males. It is, however, not known if the priming pheromones affect the ability of sperm to fertilize eggs in salmonids. In this study, we compare the proportion of larvae produced from in vitro fertilization tests between primed brown trout (Salmo trutta) males exposed to a mix of female urine and ovarian fluids, and control males exposed only to 0.9 % sodium chloride. We also investigate priming effects on milt yield and sperm motility. Fertilization tests with sperm from single males, as well as sperm from two males (i.e., sperm competition), were performed. Primed males generated more larvae in both the single male and competition fertilization tests. No differences between treatments in milt yield and sperm motility could be established.
authors.affiliation: Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences, Umeå, Sweden. gustav.hellstrom@slu.se
authors.forename: Gustav
authors.initials: G
authors.lastname: Hellström
authors.forename: Tore
authors.initials: T
authors.lastname: Prestegaard
authors.forename: Johan
authors.initials: J
authors.lastname: Dannewitz
authors.forename: K Håkan
authors.initials: KH
authors.lastname: Olsén
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods*; D005260:Female; D005298:Fertility / Q000187:drug effects*; D005306:Fertilization / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000662:veterinary; D015571:Follicular Fluid / Q000737:chemistry; D007814:Larva / Q000502:physiology; D008297:Male; D012724:Sex Attractants / Q000032:analysis / Q000494:pharmacology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000502:physiology*; D013548:Sweden; D014337:Trout / Q000502:physiology*; D014556:Urine / Q000737:chemistry
chemical_list: D012724:Sex Attractants