title: Extensive dissemination of CTX-M-1- and CMY-2-producing Escherichia coli in poultry farms in Tunisia.
abstract: We characterized 67 Escherichia coli isolates with reduced susceptibility to cefotaxime obtained from 136 samples of healthy broilers housed in 36 Tunisian farms. All these isolates harboured blaCTX-M-1 and/or blaCMY-2 genes located mostly on self-conjugative IncI1 plasmids. qnrS1, qnrA6 and aac(6')-Ib-cr were detected in six isolates. Considerable genetic diversity was detected among isolates from different farms. To our knowledge, this is the first detailed documentation of a high occurrence of blaCTX-M-1 and blaCMY-2 in E. coli at the poultry farm level in Tunisia as well as the first description of plasmid-mediated quinolone resistance in food animals in Tunisia which may contribute to the dissemination of these genes throughout Tunisia.
authors.affiliation: Laboratory of Microbiology, University Hospital Center of Habib Bourguiba, Sfax, Tunisia.
authors.forename: B
authors.initials: B
authors.lastname: Mnif
authors.affiliation: Laboratory of Microbiology, University Hospital Center of Habib Bourguiba, Sfax, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Ktari
authors.affiliation: Laboratory of Microbiology, University Hospital Center of Habib Bourguiba, Sfax, Tunisia.
authors.forename: F M
authors.initials: FM
authors.lastname: Rhimi
authors.affiliation: Laboratory of Microbiology, University Hospital Center of Habib Bourguiba, Sfax, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Hammami
mesh_terms: D000818:Animals; D002439:Cefotaxime / Q000494:pharmacology; D002645:Chickens / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D015363:Quinolones / Q000494:pharmacology; D014416:Tunisia; D001618:beta-Lactamases / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D015363:Quinolones; C103022:beta-lactamase CMY-2; C055184:beta-lactamase TEM-3; D001618:beta-Lactamases; D002439:Cefotaxime