title: G-actin regulates the shuttling and PP1 binding of the RPEL protein Phactr1 to control actomyosin assembly.
abstract: The Phactr family of PP1-binding proteins is implicated in human diseases including Parkinson's, cancer and myocardial infarction. Each Phactr protein contains four G-actin binding RPEL motifs, including an N-terminal motif, abutting a basic element, and a C-terminal triple RPEL repeat, which overlaps a conserved C-terminus required for interaction with PP1. RPEL motifs are also found in the regulatory domains of the MRTF transcriptional coactivators, where they control MRTF subcellular localisation and activity by sensing signal-induced changes in G-actin concentration. However, whether G-actin binding controls Phactr protein function - and its relation to signalling - has not been investigated. Here, we show that Rho-actin signalling induced by serum stimulation promotes the nuclear accumulation of Phactr1, but not other Phactr family members. Actin binding by the three Phactr1 C-terminal RPEL motifs is required for Phactr1 cytoplasmic localisation in resting cells. Phactr1 nuclear accumulation is importin α-β dependent. G-actin and importin α-β bind competitively to nuclear import signals associated with the N- and C-terminal RPEL motifs. All four motifs are required for the inhibition of serum-induced Phactr1 nuclear accumulation when G-actin is elevated. G-actin and PP1 bind competitively to the Phactr1 C-terminal region, and Phactr1 C-terminal RPEL mutants that cannot bind G-actin induce aberrant actomyosin structures dependent on their nuclear accumulation and on PP1 binding. In CHL-1 melanoma cells, Phactr1 exhibits actin-regulated subcellular localisation and is required for stress fibre assembly, motility and invasiveness. These data support a role for Phactr1 in actomyosin assembly and suggest that Phactr1 G-actin sensing allows its coordination with F-actin availability.
authors.affiliation: Transcription Group, CRUK London Research Institute, 44 Lincoln's Inn Fields, London WC2A 3LY,  UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Wiezlak
authors.forename: Jessica
authors.initials: J
authors.lastname: Diring
authors.forename: Jasmine
authors.initials: J
authors.lastname: Abella
authors.forename: Stephane
authors.initials: S
authors.lastname: Mouilleron
authors.forename: Michael
authors.initials: M
authors.lastname: Way
authors.forename: Neil Q
authors.initials: NQ
authors.lastname: McDonald
authors.forename: Richard
authors.initials: R
authors.lastname: Treisman
mesh_terms: D000199:Actins / Q000378:metabolism*; D000205:Actomyosin / Q000378:metabolism*; D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D011485:Protein Binding
chemical_list: D000199:Actins; D015815:Cell Adhesion Molecules; C490649:Chl1 protein, mouse; D008840:Microfilament Proteins; C570134:PHACTR1 protein, human; D000205:Actomyosin