title: Allogeneic adipose-derived stem cells with low immunogenicity constructing tissue-engineered bone for repairing bone defects in pigs.
abstract: The ideal cells for tissue engineering should have the following characteristics: easy obtainment, safety, immune privilege, the capability of self-renewal, and multipotency. Adipose-derived stem cells (ADSCs) are a promising candidate. However, the immunogenicity of allogeneic mesenchymal  stem cells limits their long-term benefits. In this study, we introduced human cytomegalovirus US2/US3 gene into the ADSCs to decrease the expression of MHC I protein of ADSCs and reduce the activation of T-cells of the recipient animals. Moreover, the biosafety and biological characteristics  of ADSCs transfected with the US2/US3 genes (ADSCs-US2/US3) were similar to normal ADSCs. Then we took ADSCs-US2/US3 to construct a tissue-engineered bone for repairing bone defects in pigs and found that there were no great differences in repair effects or healing time between the allogeneic  ADSCs-US2/US3 group and the autologous ADSC group. These results suggest that allogeneic ADSCs-US2/US3 have the advantages of biological safety, low immunogenicity, and effective osteogenesis. Such barely immunogenic ADSCs will be crucial for the success of future tissue-regenerative approaches.
authors.affiliation: Department 4, State Key Laboratory of Trauma, Burn and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University, Chongqing, China.
authors.forename: Ming-liang
authors.initials: ML
authors.lastname: Ren
authors.forename: Wei
authors.initials: W
authors.lastname: Peng
authors.forename: Zai-liang
authors.initials: ZL
authors.lastname: Yang
authors.forename: Xin-jun
authors.initials: XJ
authors.lastname: Sun
authors.forename: Shi-chang
authors.initials: SC
authors.lastname: Zhang
authors.forename: Zheng-guo
authors.initials: ZG
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D001847:Bone Diseases / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000628:therapy*; D002454:Cell Differentiation; D003587:Cytomegalovirus / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis; D011817:Rabbits; D011859:Radiography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D023822:Tissue Engineering; D015899:Tomography, Emission-Computed, Single-Photon; D014184:Transplantation, Homologous; D014457:Ulna / Q000000981:diagnostic imaging; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D006023:Glycoproteins; D017874:Immediate-Early Proteins; D008565:Membrane Proteins; C118670:US2 protein, Varicellovirus; C095111:US3 protein, cytomegalovirus; D014759:Viral Envelope Proteins