title: Generation of controllable gradients in cell density.
abstract: Making the grad(ient): a gradient in cell density was generated on a substrate that was inserted into a homogeneous suspension of cells at a specific tilt angle by taking advantage of the gradual change in the number of cells available for sedimentation. Reverse gradients were also fabricated on the same substrate using multiple sedimentation procedures.
authors.affiliation: School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, 30332, USA.
authors.forename: Wenying
authors.initials: W
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Stavros
authors.initials: S
authors.lastname: Thomopoulos
authors.forename: Younan
authors.initials: Y
authors.lastname: Xia
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002499:Centrifugation, Density Gradient / Q000379:methods*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D023822:Tissue Engineering