title: The effects of particulate ambient air pollution on the murine umbilical cord and its vessels: a quantitative morphological and immunohistochemical study.
abstract: Previous studies have shown that particulate matter (PM) compromise birth weight and placental morphology. We hypothesized that exposing mice to ambient PM would affect umbilical cord (UC) morphology. To test this, mice were kept in paired open-top exposure chambers at the same location and ambient conditions but, in one chamber, the air was filtered (F) and, in the other, it was not (NF). UCs were analysed stereologically and by immunohistochemistry to localize isoprostane and endothelin receptors. The cords of mice from NF chambers were smaller in volume due to loss of mucoid connective tissue and decrease in volume of collagen. These structural changes and in umbilical vessels were associated with greater volumes of regions immunostained for isoprostane, ET(A)R and ET(B)R. Findings indicate that the adverse effects of PM on birth weight may be mediated in part by alterations in UC structure or imbalances in the endogenous regulators of vascular tone and oxidative stress.
authors.affiliation: Laboratory of Experimental Air Pollution, School of Medicine, University of São Paulo, Brazil. verasine@usp.br
authors.forename: Mariana Matera
authors.initials: MM
authors.lastname: Veras
authors.forename: Rosane Maria
authors.initials: RM
authors.lastname: Guimarães-Silva
authors.forename: Elia Garcia
authors.initials: EG
authors.lastname: Caldini
authors.forename: Paulo H N
authors.initials: PH
authors.lastname: Saldiva
authors.forename: Marisa
authors.initials: M
authors.lastname: Dolhnikoff
authors.forename: Terry M
authors.initials: TM
authors.lastname: Mayhew
mesh_terms: D000397:Air Pollution / Q000009:adverse effects*; D000818:Animals; D003094:Collagen / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D052638:Particulate Matter / Q000633:toxicity*; D011247:Pregnancy; D044022:Receptor, Endothelin A / Q000378:metabolism; D044023:Receptor, Endothelin B / Q000378:metabolism; D014470:Umbilical Cord / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D052638:Particulate Matter; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; D003094:Collagen