title: Analyzing interactions between SSB and proteins by the use of fluorescence anisotropy.
abstract: Fluorescence anisotropy is a useful tool to detect SSB interaction with SSB binding partners. Titrating an SSB protein partner into a solution containing fluorescently labeled SSB C-terminal tail (SSB-Ct) peptide results in formation of the protein complex with molecular weights greatly higher than SSB peptide alone. Thus the tumbling of the complexes in solution is slower, and the fluorescence anisotropy (FA) signal would be increased, indicating the binding. Based on the FA binding curve, the apparent dissociation constant (K(d,app)) of the binding reaction can also be calculated.
authors.affiliation: Institute for Neurodegenerative Diseases, University of California, San Francisco, CA, USA. dlu@ind.ucsf.edu
authors.forename: Duo
authors.initials: D
authors.lastname: Lu
mesh_terms: D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D005454:Fluorescence Polarization / Q000379:methods*; D008967:Molecular Biology / Q000379:methods; D011485:Protein Binding
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins