title: Mapping of redox state of mitochondrial cytochromes in live cardiomyocytes using Raman microspectroscopy.
abstract: This paper presents a nonivasive approach to study redox state of reduced cytochromes c, c1 and b of complexes II and III in mitochondria of live cardiomyocytes by means of Raman microspectroscopy. For the first time with the proposed approach we perform studies of rod- and round-shaped cardiomyocytes, representing different morphological and functional states. Raman mapping and cluster analysis reveal that these cardiomyocytes differ in the amounts of reduced cytochromes c, c1 and b. The rod-shaped cardiomyocytes possess uneven distribution of reduced cytochromes c, c1 and b in cell center and periphery. Moreover, by means of Raman spectroscopy we demonstrated the decrease in the relative amounts of reduced cytochromes c, c1 and b in the rod-shaped cardiomyocytes caused by H2O2-induced oxidative stress before any visible changes. Results of Raman mapping and time-dependent study of reduced cytochromes of complexes II and III and cytochrome c in cardiomyocytes are in a good agreement with our fluorescence indicator studies and other published data.
authors.affiliation: Biological Faculty, Moscow State University, Russia.
authors.forename: Nadezda A
authors.initials: NA
authors.lastname: Brazhe
authors.forename: Marek
authors.initials: M
authors.lastname: Treiman
authors.forename: Alexey R
authors.initials: AR
authors.lastname: Brazhe
authors.forename: Ninett L
authors.initials: NL
authors.lastname: Find
authors.forename: Georgy V
authors.initials: GV
authors.lastname: Maksimov
authors.forename: Olga V
authors.initials: OV
authors.lastname: Sosnovtseva
mesh_terms: D000818:Animals; D003580:Cytochromes / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical; D008564:Membrane Potentials; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*; D015233:Models, Statistical; D032383:Myocytes, Cardiac / Q000166:cytology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013997:Time Factors
chemical_list: D003580:Cytochromes; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide