title: Gastrodin protect primary cultured rat hippocampal neurons against amyloid-beta peptide-induced neurotoxicity via ERK1/2-Nrf2 pathway.
abstract: One hallmark of Alzheimer's disease (AD) is amyloid-beta (Aβ) deposition, which can initiate a cascade of oxidative events that may result in neuronal death. The present study aimed to investigate the protective effects of gastrodin, a phenolic compound which shows antioxidant activity, on Aβ(1-42)-induced neurotoxicity and the underlying mechanism for this neuroprotection. Results indicate that Aβ(1-42)-induced neuronal toxicity as measured by cell viability, which was correlated with decreased catalase (CAT) content and superoxide dismutase (SOD) activity. Pre-treatment of primary hippocampal neurons with gastrodin significantly attenuated Aβ(1-42)-induced neurotoxicity and changes in SOD and CAT, and upregulated gene expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and extracellular signal-regulated kinases 1 and 2 (ERK1/2) phosphorylation. Pharmacological blockade of ERK1/2 abrogation this action of gastrodin. The ERK1/2 pathway may be involved in the neuroprotective effect of gastrodin against Aβ(1-42)-induced oxidative in primary cultured rat hippocampal neurons. These findings suggest that gastrodin could be of importance for the treatment of AD and other oxidative stress-related diseases.
authors.affiliation: The Hub Laboratory, Qiqihar Medical Univeristy, 333 BuKui Street, JianHua District, Qiqihar 161006, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zou
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.forename: Hongyan
authors.initials: H
authors.lastname: Guo
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Zhang
authors.forename: Miaoxian
authors.initials: M
authors.lastname: Dong
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001592:Benzyl Alcohols / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004622:Embryo, Mammalian; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects*; D010446:Peptide Fragments / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D016229:Amyloid beta-Peptides; D000975:Antioxidants; D001592:Benzyl Alcohols; D004791:Enzyme Inhibitors; D005419:Flavonoids; D005960:Glucosides; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42); C045345:gastrodin; D013482:Superoxide Dismutase; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one