title: Anti-diabetic activity peptides from albumin against α-glucosidase and α-amylase.
abstract: The objectives of this study were to identify novel peptides from albumin, and to evaluate and validate the anti-diabetic activity of peptides against α-glucosidase and α-amylase. In the research, albumin hydrolysate was purified and identified, tandem MS was adapted to characterise the amino acid sequences of peptides from the hydrolysate. In addition, anti-diabetic effects of the peptides with α-glucosidase and α-amylase inhibitory activity have been performed. The present work found eight novel peptides from albumin. Results also suggested that peptide KLPGF had α-glucosidase inhibitory activity with an IC(50) of 59.5±5.7μmoll(-1) and α-amylase inhibitory activity with an IC(50) of 120.0±4.0μmoll(-1). In conclusion, the results revealed that the peptide KLPGF was a potential anti-diabetic inhibitor.
authors.affiliation: Jilin University, Changchun 130012, PR China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Yu
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Yin
authors.forename: Wenzhu
authors.initials: W
authors.lastname: Zhao
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D000418:Albumins / Q000737:chemistry*; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology; D001426:Bacterial Proteins / Q000032:analysis / Q000037:antagonists & inhibitors; D005656:Fungal Proteins / Q000032:analysis / Q000037:antagonists & inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D000516:alpha-Amylases / Q000032:analysis / Q000037:antagonists & inhibitors*; D000520:alpha-Glucosidases / Q000032:analysis
chemical_list: D000418:Albumins; D001426:Bacterial Proteins; D005656:Fungal Proteins; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D010455:Peptides; D000516:alpha-Amylases; D000520:alpha-Glucosidases