title: Myosin-cross-reactive antigens from four different lactic acid bacteria are fatty acid hydratases.
abstract: The 67 kDa myosin-cross-reactive antigen (MCRA) is a member of the MCRA family of proteins present in a wide range of bacteria and was predicted to have fatty acid isomerase function. We have now characterised the catalytic activity of MCRAs from four LAB stains, including Lactobacillus rhamnosus LGG, L. plantarum ST-III, L. acidophilus NCFM and Bifidobacterium animalis subsp. lactis BB-12. MCRA genes from these strains were cloned and expressed in Escherichia coli, and the recombinant protein function was analysed with lipid profiles by GC-MS. The four MCRAs catalysed the conversion of linoleic acid and oleic acid to their respective 10-hydroxy derivatives, which suggests that MCRA proteins catalyse the first step in conjugated linoleic acid production. This is the first report of MCRA from L. rhamnosus with such catalytic function.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, People's Republic of China. njyang2006@gmail.com
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Haiqin
authors.initials: H
authors.lastname: Chen
authors.forename: Yuanda
authors.initials: Y
authors.lastname: Song
authors.forename: Yong Q
authors.initials: YQ
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D003429:Cross Reactions; D004926:Escherichia coli / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D006836:Hydro-Lyases / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D007778:Lactobacillus / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D008041:Linoleic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D019301:Oleic Acid / Q000378:metabolism; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D020539:Sequence Analysis, Protein
chemical_list: D000942:Antigens, Bacterial; D008041:Linoleic Acids; D011994:Recombinant Proteins; D019301:Oleic Acid; D006836:Hydro-Lyases