title: Effects of topographical and mechanical property alterations induced by oxygen plasma modification on stem cell behavior.
abstract: Polymeric substrates intended for cell culture and tissue engineering are often surface-modified to facilitate cell attachment of most anchorage-dependent cell types. The modification alters the surface chemistry and possibly topography. However, scant attention has been paid to other surface property alterations. In studying oxygen plasma treatment of polydimethylsiloxane (PDMS), we show that oxygen plasma treatment alters the surface chemistry and, consequently, the topography and elasticity of PDMS at the nanoscale level. The elasticity factor has the predominant effect, compared with the chemical and topographical factors, on cell adhesions of human mesenchymal stem cells (hMSCs). The enhanced focal adhesions favor cell spreading and osteogenesis of hMSCs. Given the prevalent use of PDMS in biomedical device construction and cell culture experiments, this study highlights the importance of understanding how oxygen plasma treatment would impact subsequent cell-substrate interactions. It helps explain inconsistency in the literature and guides preparation of PDMS-based biomedical devices in the future.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.forename: Karina
authors.initials: K
authors.lastname: Kulangara
authors.forename: Ruby T S
authors.initials: RT
authors.lastname: Lam
authors.forename: Rena
authors.initials: R
authors.lastname: Dharmawan
authors.forename: Kam W
authors.initials: KW
authors.lastname: Leong
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D048429:Cell Size; D002478:Cells, Cultured; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D055119:Elastic Modulus / Q000502:physiology; D006244:Hardness / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010100:Oxygen / Q000737:chemistry*; D010316:Particle Size; D058626:Plasma Gases / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology*; D013499:Surface Properties
chemical_list: D004129:Dimethylpolysiloxanes; D058626:Plasma Gases; C013830:baysilon; D010100:Oxygen