title: Suppression of matrix ions by N-phosphorylation labeling using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry.
abstract: N-Phosphorylation labeling was utilized to analyze small metabolites using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). The incorporation of a neutral phosphoryl group with high gas-phase proton affinity not only improved the ionization efficiency of the target molecules, but also greatly reduced the matrix background interference.
authors.affiliation: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong SAR, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Zhi
authors.initials: Z
authors.lastname: Tang
authors.forename: Minghua
authors.initials: M
authors.lastname: Lu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Liu
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yufen
authors.initials: Y
authors.lastname: Zhao
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives / Q000032:analysis* / Q000138:chemical synthesis; D007477:Ions / Q000032:analysis / Q000138:chemical synthesis; D015394:Molecular Structure; D010766:Phosphorylation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013194:Staining and Labeling
chemical_list: D007477:Ions; D000409:Alanine