title: Metal-chelating nanopolymers for antibody purification from human plasma.
abstract: The purification of immunoglobulin G (IgG) from human plasma was performed by using a novel metal-chelated adsorbent with nano size. The non-porous nanoparticles were produced by surfactant free emulsion polymerization of ethylene glycol dimethacrylate (EDMA) and 2-methacryloylamidohistidine (MAH). Then, Cu(II) ions were chelated on the nanoparticles. The nano-poly(EDMA-MAH) nanoparticles were characterized by Fourier transform infrared, scanning electron microscope, atomic force microscope and elemental analysis. The non-porous nanoparticles were spherical form and have 100-250 nm size distribution. The maximum IgG adsorption capacity of the Cu(II) chelated nanoparticles was found to be 463 mg/g polymer at pH 7.0 in HEPES buffer. Desorption of IgG was performed by 1.0 M NaCl and desorption rate was found to be 97 %. IgG was obtained from human plasma with purity of 94 % (up to 578 mg/g polymer). The non-porous nanoparticles allowed one-step purification of IgG from human plasma.
authors.affiliation: Faculty of Science and Arts, Chemistry Department, Adnan Menderes University, Aydin, Turkey.
authors.forename: Deniz Aktaş
authors.initials: DA
authors.lastname: Uygun
authors.forename: R Hilal
authors.initials: RH
authors.lastname: Şenay
authors.forename: Ceren
authors.initials: C
authors.lastname: Türkcan
authors.forename: Sinan
authors.initials: S
authors.lastname: Akgöl
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D002614:Chelating Agents / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D053758:Nanoparticles / Q000737:chemistry*; D010949:Plasma / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
chemical_list: D002614:Chelating Agents; D007074:Immunoglobulin G; D011108:Polymers; D003300:Copper