title: Chitosan/poly(DL,lactide-co-glycolide) scaffolds for tissue engineering.
abstract: Chitosan/poly(DL-lactide-co-glycolide) (Ch/DL PLG) composite scaffolds were fabricated by freeze-drying lyophilization, and were evaluated and compared for use as a bone regeneration scaffold through measurements of the compression mechanical properties of the porous scaffolds. Also, In vitro cell culture of Sprague-Dawley rat's osteoblasts were used to evaluate the phenotype expression of cells in the scaffolds, characterizing the cellular adhesion, proliferation and alkaline phosphatase activity. The gene expression of osteocalcin, sialoprotein, alkaline phosphatase, Type I collagen and TGFβ1 were confirmed in the samples; moreover, it was confirmed, the mineralization by IR spectra and EDS analysis. Our results thus show that Ch/DL PLG scaffolds are suitable for biological applications.
authors.affiliation: Instituto de Ingeniería y Tecnología, Universidad Autónoma de Cd. Juárez, Av. Del Charro 450 Norte, Col. Partido Romero, 32310, Cd. Juárez, CHIH, Mexico. mizul@yahoo.com
authors.forename: S A
authors.initials: SA
authors.lastname: Martel-Estrada
authors.forename: I
authors.initials: I
authors.lastname: Olivas-Armendáriz
authors.forename: C A
authors.initials: CA
authors.lastname: Martínez-Pérez
authors.forename: T
authors.initials: T
authors.lastname: Hernández
authors.forename: E I
authors.initials: EI
authors.lastname: Acosta-Gómez
authors.forename: J G
authors.initials: JG
authors.lastname: Chacón-Nava
authors.forename: F
authors.initials: F
authors.lastname: Jiménez-Vega
authors.forename: P E
authors.initials: PE
authors.lastname: García-Casillas
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D024042:Collagen Type I / Q000378:metabolism; D005612:Freeze Drying; D008297:Male; D008422:Materials Testing / Q000379:methods*; D010006:Osteoblasts / Q000378:metabolism; D015675:Osteocalcin / Q000096:biosynthesis; D010641:Phenotype; D011098:Polyglactin 910 / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012795:Sialoglycoproteins / Q000096:biosynthesis; D023822:Tissue Engineering / Q000379:methods*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D012795:Sialoglycoproteins; D053773:Transforming Growth Factor beta1; D015675:Osteocalcin; D011098:Polyglactin 910; D048271:Chitosan; D000469:Alkaline Phosphatase