title: G801A polymorphism of human stromal cell-derived factor 1 gene raises no susceptibility to neoplastic lesions of uterine cervix.
abstract: OBJECTIVE
  This study aimed to investigate the association of stromal cell-derived factor 1 (SDF-1) gene polymorphisms with the neoplastic lesions of uterine cervix in Mid-Taiwan women.
  
  
  METHODS
  Four hundred ninety-eight blood samples were collected from 161 patients with neoplasia of uterine cervix, including 76 cancer patients, 61 patients with high-grade dysplasia, and 24 with low-grade dysplasia, and 337 healthy controls who lived in Mid-Taiwan. Polymorphism of the SDF-1 gene was examined using polymerase chain reaction-restriction fragment length polymorphism.
  
  
  RESULTS
  For SDF-1 gene polymorphisms, the wild-type homozygous alleles (G/G) yielded 100- and 193-bp products, the heterozygous alleles (G/A) yielded 100-, 193- and 293-bp products, whereas the mutated-type homozygous alleles (A/A) yielded a 293-bp product. We found no significant difference in genotypes or alleles distribution of SDF-1 polymorphisms between patients with cervical neoplasia and healthy women (P = 0.530). Compared with the homozygous GG subgroup, GA and AA subgroups do not increase the risk of cervical neoplasia.
  
  
  CONCLUSIONS
  Although the expression of SDF-1 was reported to be significantly increased in cervical carcinogenesis in previous studies, our results, however, show that SDF-1 gene polymorphism could not be considered as a factor related to an increased susceptibility to cervical neoplasia.
authors.affiliation: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
authors.forename: Yi-Torng
authors.initials: YT
authors.lastname: Tee
authors.forename: Shun-Fa
authors.initials: SF
authors.lastname: Yang
authors.forename: Po-Hui
authors.initials: PH
authors.lastname: Wang
authors.forename: Hsiu-Ting
authors.initials: HT
authors.lastname: Tsai
authors.forename: Long-Yau
authors.initials: LY
authors.lastname: Lin
authors.forename: Shu-Kuei
authors.initials: SK
authors.lastname: Lee
authors.forename: Chiung-Ling
authors.initials: CL
authors.lastname: Liao
authors.forename: Jinghau Tsai
authors.initials: JT
authors.lastname: Chang
authors.forename: Yang-Tse
authors.initials: YT
authors.lastname: Shih
mesh_terms: D016022:Case-Control Studies; D002584:Cervix Uteri / Q000473:pathology*; D054377:Chemokine CXCL12 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D013624:Taiwan; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12