title: Expression of the CD2AP adaptor molecule in normal, reactive and neoplastic human tissue.
abstract: OBJECTIVE
  To study the expression of CD2-associated protein (CD2AP), an adaptor protein involved in T-cell signalling and renal function, in normal, reactive and neoplastic human lymphoid tissues.
  
  
  RESULTS
  We used immunohistochemical techniques to evaluate monoclonal antibodies against CD2AP on over 400 formalin fixed paraffin embedded tissue blocks retrieved from the host institutions of three authors. The samples tested included normal, reactive and neoplastic lymphoid tissue. In lymphoid tissues, strong CD2AP staining was observed in plasmacytoid dendritic cells (pDCs), weak and variable in mantle zone B cells and moderate in rare germinal center cells. CD2AP labeled cortical and rare medullary thymocytes and isolated mononuclear cells in bone marrow trephines. Furthermore, epithelial and endothelial cells expressed CD2AP. Among neoplasms, the greatest number of CD2AP-positive cases were found in diffuse large B cell (21/94), NK T-cell lymphomas (7/67), "blastic plasmacytoid dendritic cell neoplasms" (9/10) and some types of solid tumor.
  
  
  CONCLUSIONS
  Our finding that mature peripheral T cells are CD2AP-negative but immature cortical thymocytes are positive may prove useful for diagnostic purposes. Moreover, our results demonstrate that CD2AP represents a useful marker of normal and neoplastic pDC and may be used in a diagnostic panel in reactive or neoplastic lymphoid proliferations.
authors.affiliation: Department of Cellular Pathology, Barts and The London NHS Trust, London, UK.
authors.forename: H
authors.initials: H
authors.lastname: Rizvi
authors.forename: J C
authors.initials: JC
authors.lastname: Paterson
authors.forename: S
authors.initials: S
authors.lastname: Tedoldi
authors.forename: A
authors.initials: A
authors.lastname: Ramsay
authors.forename: M
authors.initials: M
authors.lastname: Calaminici
authors.forename: Y
authors.initials: Y
authors.lastname: Natkunam
authors.forename: S
authors.initials: S
authors.lastname: Lonardi
authors.forename: S-Y
authors.initials: SY
authors.lastname: Tan
authors.forename: L
authors.initials: L
authors.lastname: Campbell
authors.forename: M-L
authors.initials: ML
authors.lastname: Hansmann
authors.forename: D
authors.initials: D
authors.lastname: Jones
authors.forename: I
authors.initials: I
authors.lastname: Dikic
authors.forename: A S
authors.initials: AS
authors.lastname: Shaw
authors.forename: S A
authors.initials: SA
authors.lastname: Pileri
authors.forename: H
authors.initials: H
authors.lastname: Stein
authors.forename: D Y
authors.initials: DY
authors.lastname: Mason
authors.forename: F
authors.initials: F
authors.lastname: Facchetti
authors.forename: T
authors.initials: T
authors.lastname: Marafioti
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000166:cytology; D008223:Lymphoma / Q000175:diagnosis* / Q000378:metabolism*; D060168:Thymocytes / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015415:Biomarkers; C114545:CD2-associated protein; D003598:Cytoskeletal Proteins