title: Influence of size, surface coating and fine chemical composition on the in vitro reactivity and in vivo biodistribution of lipid nanocapsules versus lipid nanoemulsions in cancer models.
abstract: Lipid nanocapsules (LNCs) and lipid nanoemulsions (LNEs) are biomimetic synthetic nanocarriers. Their in vitro and in vivo performance was evaluated as a function of their size (25, 50 and 100 nm) and the surface PEG chain length. Analysis methods included complement activation test, particle uptake in macrophage and HEK293(β3) cells and biodistribution studies with tumor-grafted mice by fluorescence imaging. A particular attention was paid to keep the concentration of each nanocarrier and to the amount of fluorescent dye in comparable conditions between the in vitro and in vivo studies. Under these conditions, no significant differences were found among the three tested particle sizes and the two nanocarrier types. Longer PEG chains on the LNE surface provided better stealth properties, whereas PEG modification on the LNC formulations inhibited the production of stable nanocarriers. Passive accumulation of LNCs and LNEs in different tumor types depended on the degree of tumor vascularization.
  
  
  BACKGROUND
  This study of lipid nanocapsules and lipid nanoemulsions compares their vitro and in vivo performance as a function of size and surface PEG chain length, demonstrating no significant difference among the tested particle sizes. Longer PEG chains on the LNE surface provided better stealth properties, whereas PEG modification on the LNC formulations inhibited the production of stable nanocarriers.
authors.affiliation: INSERM U646, Angers, France.
authors.forename: Samuli
authors.initials: S
authors.lastname: Hirsjärvi
authors.forename: Sandrine
authors.initials: S
authors.lastname: Dufort
authors.forename: Julien
authors.initials: J
authors.lastname: Gravier
authors.forename: Isabelle
authors.initials: I
authors.lastname: Texier
authors.forename: Qiao
authors.initials: Q
authors.lastname: Yan
authors.forename: Jérome
authors.initials: J
authors.lastname: Bibette
authors.forename: Lucie
authors.initials: L
authors.lastname: Sancey
authors.forename: Véronique
authors.initials: V
authors.lastname: Josserand
authors.forename: Catherine
authors.initials: C
authors.lastname: Passirani
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Benoit
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Coll
mesh_terms: D000818:Animals; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000494:pharmacology; D003167:Complement Activation / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry; D004655:Emulsions / Q000493:pharmacokinetics*; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008055:Lipids / Q000493:pharmacokinetics* / Q000494:pharmacology; D051379:Mice; D008819:Mice, Nude; D053769:Nanocapsules / Q000737:chemistry*; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D010316:Particle Size; D040521:Subcutaneous Tissue / Q000187:drug effects / Q000473:pathology; D013499:Surface Properties; D014018:Tissue Distribution / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D004655:Emulsions; D008055:Lipids; D053769:Nanocapsules