title: Direct detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in food samples without derivatisation step by a sensitive and specific monoclonal antibody based ELISA.
abstract: AMOZ (3-amino-5-methylmorpholino-2-oxazolidinone) is the metabolite of furaltadone (FTD) which has been banned as a veterinary drug. In this study, three AMOZ derivatives (CPAMOZ) were synthesised and monoclonal antibody against o-nitrobenzaldehyde derivatived AMOZ (NPAMOZ) was produced. The IC(50) value of the ELISA for NPAMOZ was 0.17ngml(-1). The cross reactivity values of the assay with NPAMOZ, CPAMOZ, AMOZ and FTA were 100%, 61.7%, 14.8% and 10.4%, respectively. As no NPAMOZ or CPAMOZ exist if no derivitizing reagent added and FTA is quite unstable, therefore, 14.8% of cross reactivity with AMOZ makes the ELISA capable of directly detecting AMOZ without derivatisation. AMOZ spiked food samples were analysed by ELISA. Recoveries of 72.6-121.2% and intra-assay coefficients of variation of 6.1-17.7% were obtained. The proposed ELISA which also confirmed by HPLC with a good correlation provides an alternative sensitive and fast quantitative method for directly detecting AMOZ in food samples.
authors.affiliation: College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Wang
authors.forename: Wenhui
authors.initials: W
authors.lastname: Si
authors.forename: Anping
authors.initials: A
authors.lastname: Deng
mesh_terms: D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D023303:Oxazolidinones / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D023303:Oxazolidinones; C000209:3-amino-2-oxazolidinone