title: Determination of the kinetics and thermodynamics of ligand binding to a specific inactive conformation in protein kinases.
abstract: Recent interest in inactive kinase conformations has generated the need to develop new biochemical tools to study them. Here, we describe the use of a fluorescent probe that selectively and potently binds to a specific inactive conformation of protein kinases. This allows for the thermodynamics and kinetics of ligand binding to be determined.
authors.affiliation: Department of Chemistry, University of Washington, Seattle, WA, USA, hari1@u.washington.edu.
authors.forename: Sanjay B
authors.initials: SB
authors.lastname: Hari
authors.forename: Pratistha
authors.initials: P
authors.lastname: Ranjitkar
authors.forename: Dustin J
authors.initials: DJ
authors.lastname: Maly
mesh_terms: D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D011487:Protein Conformation; D011494:Protein Kinases / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D008024:Ligands; D011494:Protein Kinases