title: A green method using a micellar system for determination of andrographolide and dehydroandrographolide in human plasma.
abstract: A method based on cloud point extraction (CPE) coupled with high-performance liquid chromatography separation and ultraviolet (UV) detection was developed to determine andrographolide and dehydroandrographolide in human plasma. The nonionic surfactant Triton X-114 was chosen as the extraction medium. Variable parameters affecting the CPE efficiency were evaluated and optimized, such as concentrations of Triton X-114 and NaCl, pH, equilibration temperature and equilibration time. A Zorbax SB C18 column (250 × 4.6 mm i.d., 5 µm) was used for separation of the two analytes at 30°C. The UV detection was performed at 254 nm. Under the optimum conditions, the limits of detection of andrographolide and dehydroandrographolide are 0.032 and 0.019 µg/mL, respectively. The intra-day and inter-day precisions expressed as relative standard deviation ranged from 3.2 to 7.3% and from 2.9 and 8.6%. The recoveries of andrographolide and dehydroandrographolide were in the range of 76.8-98.6% at three fortified concentrations of 0.1, 0.5 and 1.0 µg/mL. This method was efficient, environmentally friendly, rapid and inexpensive for the extraction and determination of andrographolide and dehydroandrographolide in human plasma.
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Haiyan
authors.initials: H
authors.lastname: Jin
authors.forename: Lan
authors.initials: L
authors.lastname: Ding
authors.forename: Nanqi
authors.initials: N
authors.lastname: Ren
mesh_terms: D005591:Chemical Fractionation / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004224:Diterpenes / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D004355:Drug Stability; D055772:Green Chemistry Technology / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D016018:Least-Squares Analysis; D057230:Limit of Detection; D008823:Micelles; D017830:Octoxynol; D011092:Polyethylene Glycols; D015203:Reproducibility of Results; D012965:Sodium Chloride; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D004224:Diterpenes; D008823:Micelles; D011092:Polyethylene Glycols; C030419:andrographolide; D012965:Sodium Chloride; D017830:Octoxynol; C010615:Nonidet P-40; C478098:dehydroandrographolide