title: Structural studies of SSB interaction with RecO.
abstract: Interaction of recombination protein RecO with single-stranded (ss) DNA-binding protein (SSB) is essential for DNA damage repair and restart of stalled replication (Cox, Crit Rev Biochem Mol Biol 42(1):41-63, 2007). To understand mechanism of this interaction and its role in DNA repair, we deciphered a high-resolution structure of RecO complex with C-terminal tail of SSB (SSB-Ct). The structure revealed a key role of hydrophobic interactions between two proteins and suggests the mechanism of RecO recruitment to DNA during homologous recombination and strand annealing.
authors.affiliation: Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, St. Louis, MO, USA.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Ryzhikov
authors.forename: Sergey
authors.initials: S
authors.lastname: Korolev
mesh_terms: D001665:Binding Sites; D003461:Crystallography / Q000379:methods*; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism*; D005454:Fluorescence Polarization; D057927:Hydrophobic and Hydrophilic Interactions; D008967:Molecular Biology / Q000379:methods; D011995:Recombination, Genetic
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C081006:RecO protein, E coli