title: Two novel short C-type lectin from Chinese mitten crab, Eriocheir sinensis, are induced in response to LPS challenged.
abstract: The basic mechanism of host fighting against pathogens is pattern recognition receptors recognized pathogen-associated molecular patterns. However, the specificity of recognition within the innate immune molecular of invertebrates remains largely unknown. For this reason, we investigated the immune functionality of two pattern recognition receptors, C-type lectin EsLecA and EsLecG, post lipopolysaccharides (LPS) challenge in Chinese mitten crab (Eriocheir sinensis), which is a commercially important and disease vulnerable aquaculture species. The cloning of full-length EsLecA and EsLecG cDNA were based on the initial expressed sequence tags (EST) isolated from a hepatopancreatic cDNA library via PCR. The EsLecA cDNA contained a 480-bp open reading frame that encoded a putative 159-amino-acid protein, while EsLecG cDNA contained a 465-bp open reading frame that encoded a putative 154-amino-acid protein. Comparison, with other reported invertebrate and vertebrate sequences, revealed the presence of carbohydrate recognition domains that were common among C-type lectin superfamilies. EsLecA and EsLecG mRNA expression in E. sinensis were (a) both detected in all tissues, including the hepatopancreas, gills, hemocytes, testis, accessory gland, ovary, muscle, stomach, intestine, heart, thoracic ganglia and brain, and (b) responsive in hepatopancreas, gill, hemocytes post-LPS immuno-challenge all appeared dramatically variation. Collectively, the data presented here demonstrate the successful isolation of two novel C-type lectins from the Chinese mitten crab, and their role in the innate immune system of an invertebrate.
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China.
authors.forename: Xing-Kun
authors.initials: XK
authors.lastname: Jin
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Xiao-Nv
authors.initials: XN
authors.lastname: Guo
authors.forename: Ai-Qing
authors.initials: AQ
authors.lastname: Yu
authors.forename: Min-Hao
authors.initials: MH
authors.lastname: Wu
authors.forename: Lin
authors.initials: L
authors.lastname: He
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology*; D037181:Lectins, C-Type / Q000235:genetics* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction; D051192:Receptors, Pattern Recognition / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D051192:Receptors, Pattern Recognition