title: The production of β-glucosidases by Fusarium proliferatum NBRC109045 isolated from Vietnamese forest.
abstract: Fusarium proliferatum NBRC109045 is a filamentous fungus isolated from Vietnamese forest due to high production of β-glucosidases. Production of the enzyme was studied on varied carbon source based mediums. The highest activity was obtained in medium containing 1% corn stover + 1% wheat bran (3.31 ± 0.14 U/ml). It is interesting to note that glucose (0.69 ± 0.02 U/ml) gave higher activity and just followed by cellobiose among the di- and mono-saccharides, which is generally regarded as a universal repressor of hydrolases. We improved the zymogram method to prove that in response to various carbon sources, F. proliferatum could express various β-glucosidases. One of the β-glucosidases produced by F. proliferatum growing in corn stover + wheat bran based medium was partially purified and proved to have high catalytic ability.
authors.affiliation: Department of Agro-Environmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan. ryukondo@agr.kyushu-u.ac.jp.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Gao
authors.forename: Duong
authors.initials: D
authors.lastname: Van Hop
authors.forename: Le Thi Hoang
authors.initials: le TH
authors.lastname: Yen
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Ando
authors.forename: Shuichi
authors.initials: S
authors.lastname: Hiyamuta
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Kondo