title: Effect of therapeutic hypothermia according to severity of sepsis in a septic rat model.
abstract: OBJECTIVE
  The effects of therapeutic hypothermia (HT) during experimental sepsis may be influenced by disease severity. We experimentally investigated the effect of therapeutic HT on varying disease severity in a septic rat model.
  
  
  METHODS
  An adult male Sprague-Dawley rat model of intra-abdominal sepsis was used. To modify the disease severity, we used two different models; a moderate severe sepsis model (MSSM) and a severe septic shock model (SSSM). All rats were randomized to a hypothermia group (HT, 30-32°C) or a normothermia group (NT, 36-38°C) 1h after sepsis induction in each model. HT was maintained for 4h and rewarming was conducted for 2h. Survival time was recorded for up to 12h in the SSSM group and 24h in the MSSM group. Acute lung and liver injury, cytokine, and malondialdehyde (MDA) levels were investigated 7h after sepsis induction. Hemodynamic profiles were also evaluated.
  
  
  RESULTS
  In the SSSM, there were survival benefits and reduced acute lung and liver injury with therapeutic HT. Therapeutic HT was also associated with significantly reduced levels of plasma interleukin-6 and tissue malondialdehyde (MDA) levels in the liver and lung compared with the NT group in the SSSM. There was a tendency for the mean arterial pressure to be higher in the HT group compared to the NT group in the SSSM. In MSSM, however, there was no such beneficial effect.
  
  
  CONCLUSIONS
  In this rat model of severe septic shock, therapeutic HT showed beneficial effects. In contrast, therapeutic HT did not show protective effect in the moderate sepsis model.
authors.affiliation: Department of Emergency Medicine, Seoul Medical Center, 316 Sinnae-dong, Jungnang-gu, Seoul 131-130, Republic of Korea.
authors.forename: Kwang Pil
authors.initials: KP
authors.lastname: Rim
authors.forename: Kyuseok
authors.initials: K
authors.lastname: Kim
authors.forename: You Hwan
authors.initials: YH
authors.lastname: Jo
authors.forename: Jae Hyuk
authors.initials: JH
authors.lastname: Lee
authors.forename: Joong Eui
authors.initials: JE
authors.lastname: Rhee
authors.forename: Kyeong Won
authors.initials: KW
authors.lastname: Kang
authors.forename: Gil Joon
authors.initials: GJ
authors.lastname: Suh
authors.forename: Woon Yong
authors.initials: WY
authors.lastname: Kwon
authors.forename: Min Ji
authors.initials: MJ
authors.lastname: Lee
authors.forename: Hyun Sook
authors.initials: HS
authors.lastname: Lee
mesh_terms: D058186:Acute Kidney Injury / Q000628:therapy; D055371:Acute Lung Injury / Q000628:therapy; D000818:Animals; D062186:Arterial Pressure; D002432:Cecum / Q000601:surgery; D006979:Hyperthermia, Induced; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000293:injuries / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000628:therapy*; D012772:Shock, Septic / Q000628:therapy*
chemical_list: D015850:Interleukin-6; D016753:Interleukin-10; D008315:Malondialdehyde