title: The protective effect of Canova homeopathic medicine in cyclophosphamide-treated non-human primates.
abstract: BACKGROUND
  Canova activates macrophages and indirectly induces lymphocyte proliferation. Here we evaluated the effects of Canova in cyclophosphamide-treated non-human primates.
  
  
  METHODS
  Twelve Cebus apella were evaluated. Four animals were treated with Canova only. Eight animals were treated with two doses of cyclophosphamide (50 mg/kg) and four of these animals received Canova. Body weight, biochemistry and hematologic analyses were performed for 40 days. Micronucleus and comet assays were performed for the evaluation of DNA damage.
  
  
  RESULTS
  We observed that cyclophosphamide induced abnormal WBC count in all animals. However, the group treated with cyclophosphamide plus Canova presented a higher leukocyte count than that which received only cyclophosphamide. Cyclophosphamide induced micronucleus and DNA damage in all animals. The frequency of these alterations was significantly lower in the Canova group than in the group without this medicine.
  
  
  CONCLUSIONS
  Our results demonstrated that Canova treatment minimizes cyclophosphamide myelotoxicity in C. apella.
authors.affiliation: Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, São Paulo 04023-900, SP, Brazil. mariana.morf@epm.br
authors.forename: Mariana Ferreira
authors.initials: MF
authors.lastname: Leal
authors.forename: Lusânia Maria Greggi
authors.initials: LM
authors.lastname: Antunes
authors.forename: Maria Fernanda Vita
authors.initials: MF
authors.lastname: Lamarão
authors.forename: Carla Elvira Araújo
authors.initials: CE
authors.lastname: da Silva
authors.forename: Ismael Dale Cotrim Guerreiro
authors.initials: ID
authors.lastname: da Silva
authors.forename: Paulo Pimentel
authors.initials: PP
authors.lastname: Assumpção
authors.forename: Edilson Ferreira
authors.initials: EF
authors.lastname: Andrade
authors.forename: Alexandre Pingarilho
authors.initials: AP
authors.lastname: Rezende
authors.forename: Aline Amaral
authors.initials: AA
authors.lastname: Imbeloni
authors.forename: José Augusto Pereira Carneiro
authors.initials: JA
authors.lastname: Muniz
authors.forename: Giovanny Rebouças
authors.initials: GR
authors.lastname: Pinto
authors.forename: Marília de Arruda Cardoso
authors.initials: Mde A
authors.lastname: Smith
authors.forename: Rommel Rodríguez
authors.initials: RR
authors.lastname: Burbano
mesh_terms: D000818:Animals; D002428:Cebus; D049109:Cell Proliferation / Q000187:drug effects; D020552:Comet Assay / Q000379:methods; D003520:Cyclophosphamide / Q000009:adverse effects*; D004249:DNA Damage / Q000187:drug effects; D006705:Homeopathy; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008419:Materia Medica / Q000494:pharmacology*; D015162:Micronucleus Tests / Q000379:methods
chemical_list: D008419:Materia Medica; D003520:Cyclophosphamide