title: Effect of hemicellulase addition during enzymatic hydrolysis of switchgrass pretreated by soaking in aqueous ammonia.
abstract: The focus of this study was to determine the effect of supplementing cellulase with hemicellulase during enzymatic hydrolysis of switchgrass pretreated by soaking in aqueous ammonia (SAA) under a range of conditions. SAA was performed using 15% aqueous ammonia for 8 or 24h at temperatures of 40 or 60°C. The combined effect of cellulase and hemicellulase loadings on glucose yield during enzymatic hydrolysis was modeled for each pretreatment condition. Glucose yields greater than 85% of theoretical were achieved for pretreatment at 40°C for 24h and for 60°C for 8h. Hemicellulase supplementation was not sufficient to achieve these glucose yields at lower severity SAA pretreatment. High severity SAA pretreatment also led to low yields despite improved delignification.
authors.affiliation: Department of Agricultural and Biosystems Engineering, North Dakota State University, Fargo, ND 58108, USA. scott.pryor@ndsu.edu
authors.forename: Scott W
authors.initials: SW
authors.lastname: Pryor
authors.forename: Bishnu
authors.initials: B
authors.lastname: Karki
authors.forename: Nurun
authors.initials: N
authors.lastname: Nahar
mesh_terms: D000641:Ammonia / Q000494:pharmacology*; D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000494:pharmacology*; D006868:Hydrolysis / Q000187:drug effects; D008897:Panicum / Q000187:drug effects* / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water; D000641:Ammonia; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase; D005947:Glucose