title: Dendrimer functionalization with a membrane-interacting domain of herpes simplex virus type 1: towards intracellular delivery.
abstract: A poly(amide)-based dendrimer was synthesized and functionalized with the membrane-interacting peptide gH(625-644) (gH625) derived from the herpes simplex virus type 1 (HSV-1) envelope glycoprotein H, which has previously been shown to assist in delivering large cargoes across the cellular membrane. We demonstrate that the attachment of the gH625 peptide sequence to the termini of a dendrimer allows the conjugate to penetrate into the cellular matrix, whereas the unfunctionalized dendrimer is excluded from translocation. The peptide-functionalized dendrimer is rapidly taken into the cells mainly through a non-active translocation mechanism. Our results suggest that the presented peptidodendrimeric scaffold may be a promising material for efficient drug delivery.
authors.affiliation: Molecular Design Institute, Department of Chemistry, New York University, NY 10003, USA.
authors.forename: Tom P
authors.initials: TP
authors.lastname: Carberry
authors.forename: Rossella
authors.initials: R
authors.lastname: Tarallo
authors.forename: Annarita
authors.initials: A
authors.lastname: Falanga
authors.forename: Emiliana
authors.initials: E
authors.lastname: Finamore
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Galdiero
authors.forename: Marcus
authors.initials: M
authors.lastname: Weck
authors.forename: Stefania
authors.initials: S
authors.lastname: Galdiero
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000378:metabolism; D057930:Click Chemistry; D050091:Dendrimers / Q000138:chemical synthesis / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D018259:Herpesvirus 1, Human / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D050091:Dendrimers; D005456:Fluorescent Dyes; D014759:Viral Envelope Proteins; C044200:glycoprotein H, herpes simplex virus type 1