title: Effect of a calcium-silicate-based restorative cement on pulp repair.
abstract: In cases of pulp injury, capping materials are used to enhance tertiary dentin formation; Ca(OH)(2) and MTA are the current gold standards. The aim of this study was to evaluate the capacity of a new calcium-silicate-based restorative cement to induce pulp healing in a rat pulp injury model. For that purpose, cavities with mechanical pulp exposure were prepared on maxillary first molars of 27 six-week-old male rats, and damaged pulps were capped with either the new calcium-silicate-based restorative cement (Biodentine), MTA, or Ca(OH)(2). Cavities were sealed with glass-ionomer cement, and the repair process was assessed at several time-points. At day 7, our results showed that both the evaluated cement and MTA induced cell proliferation and formation of mineralization foci, which were strongly positive for osteopontin. At longer time-points, we observed the formation of a homogeneous dentin bridge at the injury site, secreted by cells displaying an odontoblastic phenotype. In contrast, the reparative tissue induced by Ca(OH)(2) showed porous organization, suggesting a reparative process different from those induced by calcium silicate cements. Analysis of these data suggests that the evaluated cement can be used for direct pulp-capping.
authors.affiliation: EA 2496, Pathologies, Imaging and Biotherapies of the Tooth, Dental School, Université Paris Descartes, PRES Sorbonne Paris Cité, 1 rue Maurice Arnoux 92120, France.
authors.forename: X V
authors.initials: XV
authors.lastname: Tran
authors.forename: C
authors.initials: C
authors.lastname: Gorin
authors.forename: C
authors.initials: C
authors.lastname: Willig
authors.forename: B
authors.initials: B
authors.lastname: Baroukh
authors.forename: B
authors.initials: B
authors.lastname: Pellat
authors.forename: F
authors.initials: F
authors.lastname: Decup
authors.forename: S
authors.initials: S
authors.lastname: Opsahl Vital
authors.forename: C
authors.initials: C
authors.lastname: Chaussain
authors.forename: T
authors.initials: T
authors.lastname: Boukpessi
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000627:therapeutic use; D017610:Calcium Compounds / Q000737:chemistry / Q000627:therapeutic use*; D049109:Cell Proliferation / Q000187:drug effects; D003738:Dental Cements / Q000737:chemistry / Q000627:therapeutic use; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D003793:Dental Restoration, Permanent / Q000379:methods; D003809:Dentin, Secondary / Q000187:drug effects* / Q000648:ultrastructure; D003810:Dentinogenesis / Q000187:drug effects*; D004195:Disease Models, Animal; D008137:Longitudinal Studies; D008297:Male; D010012:Osteogenesis / Q000187:drug effects; D057872:Pulp Capping and Pulpectomy Agents / Q000737:chemistry / Q000627:therapeutic use*; D051381:Rats; D017640:Silicates / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D003738:Dental Cements; D057872:Pulp Capping and Pulpectomy Agents; D017640:Silicates; C506393:tricalcium silicate; C031293:calcium silicate