title: Drosophila LSD1-CoREST demethylase complex regulates DPP/TGFβ signaling during wing development.
abstract: The choice and timing of specific developmental pathways in organogenesis are determined by tissue-specific temporal and spatial cues that are acted upon to impart unique cellular and compartmental identities. A consequence of cellular signaling is the rapid transcriptional reprogramming of a wide variety of target genes. To overcome intrinsic epigenetic chromatin barriers to transcription modulation, histone modifying and remodeling complexes are employed. The deposition or erasure of specific covalent histone modifications, including acetylation, methylation, and ubiquitination are essential features of gene activation and repression. We have found that the activity of a specific class of histone demethylation enzymes is required for the specification of vein cell fates during Drosophila wing development. Genetic tests revealed that the Drosophila LSD1-CoREST complex is required for proper cell specification through regulation of the DPP/TGFβ pathway. An important finding from this analysis is that LSD1-CoREST functions through control of rhomboid expression in an EGFR-independent pathway.
authors.affiliation: Program in Molecular and Cellular Biochemistry, Stritch School of Medicine, Loyola University of Chicago, Maywood, Illinois, USA.
authors.forename: Brenda J
authors.initials: BJ
authors.lastname: Curtis
authors.forename: Claudia B
authors.initials: CB
authors.lastname: Zraly
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Dingwall
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D056970:Co-Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology; D008565:Membrane Proteins / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D014680:Veins / Q000166:cytology; D014921:Wings, Animal / Q000254:growth & development
chemical_list: D056970:Co-Repressor Proteins; C579421:CoREST protein, Drosophila; D029721:Drosophila Proteins; D008565:Membrane Proteins; C081481:Rho protein, Drosophila; D016212:Transforming Growth Factor beta; C064196:dpp protein, Drosophila; C524804:Lsd-1 protein, Drosophila; D010089:Oxidoreductases, N-Demethylating