title: A novel gene, ardD, determines antirestriction activity of the non-conjugative transposon Tn5053 and is located antisense within the tniA gene.
abstract: The mercury-resistance transposon Tn5053 inhibits restriction activity of the type I restriction-modification endonuclease EcoKI in Escherichia coli K12 cells. This is the first report of antirestriction activity of a non-conjugative transposon. The gene (ardD) coding for the antirestriction protein has been cloned. The ardD gene is located within the tniA gene, coding for transposase, on the complementary strand. The direction of transcription is opposite to transcription of the tniA gene.
authors.affiliation: State Research Institute of Genetics and Selection of Industrial Microorganisms (GosNIIgenetika), Moscow, Russia.
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Balabanov
authors.forename: Vera Yu
authors.initials: VY
authors.lastname: Kotova
authors.forename: Gennady Y
authors.initials: GY
authors.lastname: Kholodii
authors.forename: Sofia Z
authors.initials: SZ
authors.lastname: Mindlin
authors.forename: Gennadii B
authors.initials: GB
authors.lastname: Zavilgelsky
mesh_terms: D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000037:antagonists & inhibitors*; D004251:DNA Transposable Elements; D048168:Escherichia coli K12 / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D014158:Transcription, Genetic; D019895:Transposases / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D029968:Escherichia coli Proteins; C579753:ardD protein, E coli; C473504:TniA transposase; D019895:Transposases; D004262:DNA Restriction Enzymes; C089629:endodeoxyribonuclease EcoKI