title: Genistein demethylates the promoter of CHD5 and inhibits neuroblastoma             growth in vivo.
abstract: Neuroblastoma (NB) is a type of tumor usually found in children under 5             years of age, which originates from lesions in the nervous system and has fast             growth and early transformation characteristics. Similar to other cancer types,             some typical tumor suppressor genes (TSGs), such as P53 and CHD5 are silenced             in NB because of high methylation at promoter zones. In the present study, our             results showed that genistein, an element found in soy, is an epigenetic modifier             able to decrease hypermethylation levels of CHD5, and enhances the expression             of CHD5 as well as p53, possibly contributing to inhibition of NB growth in vivo             and tumor microvessel formation. Furthermore, genistein acts as a DNA methyltransferase             (DNMT) inhibitor to significantly decrease the expression of DNMT3b. Our study             indicates that genistein plays an important role in inhibiting NB growth in vivo,             probably preventing tumorigenesis risk as a kind of therapeutic agent for NB treatment             in the future.
authors.affiliation: Department of Pediatric Surgery, Children's Hospital of Shanghai, Shanghai Jiaotong University, Shanghai 200040, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Weijue
authors.initials: W
authors.lastname: Xu
authors.forename: Yimin
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiong
authors.initials: X
authors.lastname: Huang
authors.forename: Lingcang
authors.initials: L
authors.lastname: Xu
authors.forename: Zhibao
authors.initials: Z
authors.lastname: Lv
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology* / Q000627:therapeutic use; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D018899:CpG Islands; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics / Q000378:metabolism; D004265:DNA Helicases / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation; D015870:Gene Expression / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D055806:Microvessels / Q000473:pathology; D008969:Molecular Sequence Data; D009389:Neovascularization, Pathologic; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D009447:Neuroblastoma / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays; D000097584:DNA Methyltransferase 3B
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004248:DNA (Cytosine-5-)-Methyltransferases; D004265:DNA Helicases; D019833:Genistein; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D000097584:DNA Methyltransferase 3B; C471926:CHD5 protein, human