title: Antioxidant and antihemolytic activities of flavonoid rich fractions of Artemisia tschernieviana Besser.
abstract: OBJECTIVE
  Artemisia (Asteraceae) contains more than 400 species. Many of the plants belonging to this genus are known to possess biological properties. In this study, antioxidant and antihemolytic activities of flavonoid rich fractions of A. tschernieviana Besser were evaluated.
  
  
  METHODS
  Plant aerial parts were extracted with 60% acetone. Extract was fractionated sequentially with hexane (HE), ethyl acetate (EA) and water (AQ). Antioxidant and antihemolytic activities of these fractions were assessed. Their antihemolytic activity was determined by H2O2 and cumene hydroperoxide induced hemolysis models.
  
  
  RESULTS
  AQ fraction showed very powerful activity in 1,1-disphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity with IC50 = 0.12 +/- 0.01 microg ml(-1) that was better than controls (vitamin C, quercetin and butylated hydroxyanisole (BHA)). This fraction showed very powerful reducing power assay that was better than vitamin C (p < 0.01). EA fraction showed higher activity in scavenging nitric oxide with IC50 = 0.14 +/- 0.04 microg ml(-1). In iron chelating activity HE fraction showed the best activity (IC50 = 162.2 +/- 8.7 microg ml(-1)). In scavenging of hydrogen peroxide, AQ fraction showed better activity than control group. This fraction had higher phenol and flavonoid contents. EA fraction showed higher antihemolytic activity with IC50 = 728.8 +/- 29 microg ml(-1).
  
  
  CONCLUSIONS
  The fractions show very good activities in studied models. Aqueous fractions showed better activity than the others in nearly all tested models. These results can be useful as a starting point of view for further applications of A. tschernieviana aerial parts or its constituents in pharmaceutical preparations after performing clinical in vivo researches.
authors.affiliation: Department of Biology, University of Mazandaran, Babolsar, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Naqinezhad
authors.forename: S M
authors.initials: SM
authors.lastname: Nabavi
authors.forename: S F
authors.initials: SF
authors.lastname: Nabavi
authors.forename: M A
authors.initials: MA
authors.lastname: Ebrahimzadeh
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D019306:Artemisia / Q000737:chemistry*; D001205:Ascorbic Acid / Q000494:pharmacology; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D006461:Hemolysis / Q000187:drug effects*; D020128:Inhibitory Concentration 50; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D012997:Solvents; D002083:Butylated Hydroxyanisole; D009569:Nitric Oxide; D011794:Quercetin; D001205:Ascorbic Acid