title: Generating diploid embryos from Xenopus tropicalis.
abstract: A spectacular advantage of Xenopus tropicalis is the ease with which diploid embryos can be generated year round. By the simple administration of human chorionic gonadotropin, an investigator can generate many hundreds of synchronized embryos by in vitro fertilization or thousands of embryos from a mating pair. The ability to induce ovulations when desired facilitates many different experiments such as experimental embryology, molecular manipulation of gene products, and genetics.
authors.affiliation: Department of Pediatrics and Genetics, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Florencia
authors.initials: F
authors.lastname: del Viso
authors.forename: Mustafa
authors.initials: M
authors.lastname: Khokha
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D002021:Buffers; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D003307:Copulation; D004171:Diploidy; D004625:Embryo, Nonmammalian / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D008297:Male; D010060:Ovulation; D012102:Reproductive Control Agents / Q000008:administration & dosage; D014981:Xenopus / Q000235:genetics*
chemical_list: D002021:Buffers; D006063:Chorionic Gonadotropin; D012102:Reproductive Control Agents