title: [Development of the HDL-C and LDL-C direct methods and the subsequent evolution].
abstract: Today, more than a decade after the development of direct methods for determining HDL-cholesterol (HDL-C) and LDL-cholesterol (LDL-C) concentrations, there are calls to review the reagents in response to discrepancies in patient samples with increased levels of atypical lipoproteins, such as apoE-rich HDL, IDL, Lp-X, and Lp-Y. Seven direct HDL-C assays showed different reactions toward apoE-rich HDL in sera from patients with CETP deficiency and cholestasis. On the other hand, the reactivity of the direct LDL-C assays to serum samples with elevated levels of IDL, Lp-X, and Lp-Y varied considerably between assay kits from each manufacturer. We have also examined the anti-atherogenic functions of apoE-rich HDL from the serum of healthy individuals and patients with CETP deficiency and cholestasis. The ability of apoE-rich HDL to remove cholesterol from cholesterol-loaded macrophages showed that the apoE-rich HDL from CETP-deficient serum took up more cholesterol than apoE-poor HDL (p < 0.01), but no significant differences were observed for apoE-rich HDL from patients with cholestasis.
authors.affiliation: Department of Medical Technology, Kumamoto Health Science University, Kumamoto 861-5598, Japan. sugiuchi@kumamoto-hsu.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugiuchi
authors.forename: Kazumi
authors.initials: K
authors.lastname: Matsushima
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Akiyoshi
authors.forename: Kaori
authors.initials: K
authors.lastname: Maeda
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ando
mesh_terms: D001057:Apolipoproteins E / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood*; D006801:Humans; D008074:Lipoproteins / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood
chemical_list: D001057:Apolipoproteins E; D053480:Cholesterol Ester Transfer Proteins; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D008074:Lipoproteins; D008075:Lipoproteins, HDL; C025121:apolipoprotein E-rich HDL