title: CMOS image sensor-based immunodetection by refractive-index change.
abstract: A complementary metal oxide semiconductor (CMOS) image sensor is an intriguing technology for the development of a novel biosensor. Indeed, the CMOS image sensor mechanism concerning the detection of the antigen-antibody (Ag-Ab) interaction at the nanoscale has been ambiguous so far. To understand the mechanism, more extensive research has been necessary to achieve point-of-care diagnostic devices. This research has demonstrated a CMOS image sensor-based analysis of cardiovascular disease markers, such as C-reactive protein (CRP) and troponin I, Ag-Ab interactions on indium nanoparticle (InNP) substrates by simple photon count variation. The developed sensor is feasible to detect proteins even at a fg/mL concentration under ordinary room light. Possible mechanisms, such as dielectric constant and refractive-index changes, have been studied and proposed. A dramatic change in the refractive index after protein adsorption on an InNP substrate was observed to be a predominant factor involved in CMOS image sensor-based immunoassay.
authors.affiliation: Department of Bionanotechnology, Gachon University, Gyeonggi-Do, Republic of Korea.
authors.forename: Jasmine P
authors.initials: JP
authors.lastname: Devadhasan
authors.forename: Sanghyo
authors.initials: S
authors.lastname: Kim
mesh_terms: D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D002097:C-Reactive Protein / Q000032:analysis*; D007204:Indium / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D012666:Semiconductors; D019210:Troponin I / Q000032:analysis*
chemical_list: D010087:Oxides; D019210:Troponin I; D007204:Indium; D002097:C-Reactive Protein