title: VSTM1-v2, a novel soluble glycoprotein, promotes the differentiation and activation of Th17 cells.
abstract: Cytokines are soluble proteins that mediate immune reactions and are responsible for communication among immune cells. CD4(+) T cells are the principle sources of cytokines of adaptive immunity. Cytokines play critical roles in the differentiation and effector function of CD4(+) T cells. They also play key roles in diseases, and some of them have been developed into drugs in the forms of recombinant cytokines, soluble receptors and neutralizing antibodies. Therefore, identifying novel potential cytokines is necessary and beneficial for better understanding immunology and enhancing human health. To find novel potential cytokines, we carried out an integrated bioinformatics analysis on the whole human genome. Cytokine candidates were selected for cDNA cloning, sub-cloning, secretion verification, expression profile analysis and functional study. Here, we report a novel soluble protein, VSTM1-v2, which is a classical secretory glycoprotein mainly expressed in immune tissues, and can promote the differentiation and activation of Th17 cells.
authors.affiliation: Peking University Center for Human Disease Genomics, Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health, School of Basic Medical Sciences, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100191, China.
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Guo
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Pingzhang
authors.initials: P
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.forename: Weiwei
authors.initials: W
authors.lastname: Fu
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Mo
authors.forename: Taiping
authors.initials: T
authors.lastname: Shi
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Chen
authors.forename: Dalong
authors.initials: D
authors.lastname: Ma
authors.forename: Wenling
authors.initials: W
authors.lastname: Han
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D003001:Cloning, Molecular; D016207:Cytokines / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D015870:Gene Expression; D015723:Gene Library; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D020381:Interleukin-17 / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits; D011971:Receptors, Immunologic / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D012995:Solubility; D058504:Th17 Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D014162:Transfection
chemical_list: D016207:Cytokines; D006023:Glycoproteins; C508717:IL17A protein, human; D020381:Interleukin-17; D020033:Protein Isoforms; D011971:Receptors, Immunologic; C550469:VSTM1 protein, human