title: Translation of biomechanical concepts in bone tissue engineering: from animal study to revision knee arthroplasty.
abstract: Bone defects in revision knee arthroplasty are often located in load-bearing regions. The goal of this study was to determine whether a physiologic load could be used as an in situ osteogenic signal to the scaffolds filling the bone defects. In order to answer this question, we proposed a novel translation procedure having four steps: (1) determining the mechanical stimulus using finite element method, (2) designing an animal study to measure bone formation spatially and temporally using micro-CT imaging in the scaffold subjected to the estimated mechanical stimulus, (3) identifying bone formation parameters for the loaded and non-loaded cases appearing in a recently developed mathematical model for bone formation in the scaffold and (4) estimating the stiffness and the bone formation in the bone-scaffold construct. With this procedure, we estimated that after 3 years mechanical stimulation increases the bone volume fraction and the stiffness of scaffold by 1.5- and 2.7-fold, respectively, compared to a non-loaded situation.
authors.affiliation: a Laboratory of Biomechanical Orthopedics, Ecole Polytechnique Fédérale de Lausanne , STI, IBI, Station 19, 1015 Lausanne , Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Roshan-Ghias
authors.forename: A
authors.initials: A
authors.lastname: Terrier
authors.forename: B M
authors.initials: BM
authors.lastname: Jolles
authors.forename: D P
authors.initials: DP
authors.lastname: Pioletti
mesh_terms: D000818:Animals; D019645:Arthroplasty, Replacement, Knee; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000502:physiology; D020342:Finite Element Analysis; D008954:Models, Biological; D010012:Osteogenesis; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D016474:Weight-Bearing / Q000502:physiology; D055114:X-Ray Microtomography