title: Real-time and in-situ cell imaging of thiol compounds in living cells using maleimide BODIPY labeling.
abstract: In this paper, a simple analytical method for the visualization of thiol compounds including glutathione (GSH), N-acetylcysteine (NAC), cysteine (Cys) and coenzyme A (CoA) in living cells is developed based on 1,3,5,7-tetramethyl-8-phenyl-(2-maleimide)-difluoroboradiaza-s-indacene (TMPAB-o-M) labeling. By using of this fluorogenic reagent, in-situ imaging of thiol compounds in cells could be achieved in only 90 s, which is much faster than that mentioned in other reports. The fluorescence of derivative products in living cells could be stable for atleast 15 min under irradiation, and can be quantified by HPLC easily in only 6 min.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Guo
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Zhang
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Guo
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival; D005240:Feasibility Studies; D006575:Heterocyclic Compounds, 3-Ring / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008301:Maleimides / Q000737:chemistry* / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D013194:Staining and Labeling; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013997:Time Factors
chemical_list: C534661:1,3,5,7-tetramethyl-8-phenyl-(2-maleimide)-difluoroboradiaza-s-indacene; C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D006575:Heterocyclic Compounds, 3-Ring; D008301:Maleimides; D013438:Sulfhydryl Compounds