title: MultiBac complexomics.
abstract: Recombinant production of multiprotein complexes is an emerging focus in academic and pharmaceutical research and is expected to play a key role in addressing complex biological questions in health and disease. Here we describe MultiBac, a state-of-the-art eukaryotic expression technology utilizing an engineered baculovirus to infect insect cells. The robust and flexible concept of MultiBac allows for simultaneous expression of multiple proteins in a single cell, which can be used to produce protein complexes and to recapitulate metabolic pathways. The MultiBac system has been set up as an open-access platform technology at the European Molecular Biology Laboratory (EMBL) in Grenoble, France. The performance of this platform and its access modalities to the scientific community are detailed in this article. The MultiBac system has been instrumental for unlocking the function of a number of essential multiprotein complexes and recent examples are discussed. This article presents a novel concept for the customized production of glycosylated protein targets using SweetBac, a modified MultiBac vector system. Finally, this article outlines how MultiBac may further develop in the future to serve applications in both academic and industrial research and development.
authors.affiliation: European Molecular Biology Laboratory and Unit of Virus Host Cell Interactions, CNRS-EMBL-UJF UMR 5322, 6 rue Jules Horowitz, F-38042 Grenoble Cedex 9, France.
authors.forename: Simon
authors.initials: S
authors.lastname: Trowitzsch
authors.forename: Dieter
authors.initials: D
authors.lastname: Palmberger
authors.forename: Daniel
authors.initials: D
authors.lastname: Fitzgerald
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Takagi
authors.forename: Imre
authors.initials: I
authors.lastname: Berger
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D015153:Blotting, Western; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D016679:Genome, Viral; D006023:Glycoproteins / Q000235:genetics; D046912:Multiprotein Complexes / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D061987:Sf9 Cells
chemical_list: D006023:Glycoproteins; D046912:Multiprotein Complexes; D011994:Recombinant Proteins