title: Atypical Leydig cell hyperplasia in adult rats with low T and high LH induced by prenatal Di(n-butyl) phthalate exposure.
abstract: The present study describes atypical Leydig cell (LC) hyperplasia in 20-week-old Sprague-Dawley rats with low testosterone and high luteinizing hormone levels after prenatal administration of 100 mg/kg/day di(n-butyl) phthalate on days 12 to 21 postconception. Light microscopy revealed LC hyperplasia surrounded by severely degenerated seminiferous tubules. Aggregated LCs had large ovoid nuclei with nucleoli and abundant eosinophilic cytoplasm. Immunohistochemical analysis showed expression of proliferating cell nuclear antigen and vimentin in many hyperplastic LCs. Electron microscopy revealed atypical nuclei, abundant free ribosomes, stripped rough endoplasmic reticulum, intermediate-size filaments, elongated cytoplasmic filopodia, atypical tight junctions, and cilia formations, but smooth endoplasmic reticulum was scarcely observed.
authors.affiliation: Department of Toxicology and Laboratory Animal Science, Azabu University School of Veterinary Medicine, Kanagawa, Japan. wakui@azabu-u.ac.jp
authors.forename: Shin
authors.initials: S
authors.lastname: Wakui
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takahashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Mutou
authors.forename: Masaru
authors.initials: M
authors.lastname: Shirai
authors.forename: Promsuk
authors.initials: P
authors.lastname: Jutabha
authors.forename: Naohiko
authors.initials: N
authors.lastname: Anzai
authors.forename: Michael F
authors.initials: MF
authors.lastname: Wempe
authors.forename: Norio
authors.initials: N
authors.lastname: Kansaku
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hano
authors.forename: Tomo
authors.initials: T
authors.lastname: Inomata
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Endou
mesh_terms: D000818:Animals; D003993:Dibutyl Phthalate / Q000633:toxicity*; D005260:Female; D006651:Histocytochemistry; D006965:Hyperplasia / Q000139:chemically induced / Q000473:pathology; D007985:Leydig Cells / Q000187:drug effects* / Q000473:pathology*; D007986:Luteinizing Hormone / Q000097:blood*; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced* / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013737:Testis / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D013739:Testosterone / Q000378:metabolism*
chemical_list: D003993:Dibutyl Phthalate; D013739:Testosterone; D007986:Luteinizing Hormone