title: Molecular characterization and immunological roles of avian IL-22 and its soluble receptor IL-22 binding protein.
abstract: As a member of the interleukin (IL)-10 family, IL-22 is an important mediator in modulating tissue responses during inflammation. Through activation of STAT3-signaling cascades, IL-22 induces proliferative and anti-apoptotic pathways, as well as antimicrobial peptides (AMPs), that help prevent tissue damage and aid in its repair. This study reports the cloning and expression of recombinant chicken IL-22 (rChIL-22) and its soluble receptor, rChIL22BP, and characterization of biological effects of rChIL-22 during inflammatory responses. Similar to observations with mammalian IL-22, purified rChIL-22 had no effect on either peripheral blood mononuclear cells (PBMCs) or lymphocytes. This was due to the low expression of the receptor ChIL22RA1 chain compared to ChIL10RB chain. rChIL-22 alone did not affect chicken embryo kidney cells (CEKCs); however, co-stimulation of CEKCs with LPS and rChIL-22 enhanced the production of pro-inflammatory cytokines, chemokines and AMPs. Furthermore, rChIL-22 alone stimulated and induced acute phase reactants in chicken embryo liver cells (CELCs). These effects of rChIL-22 were abolished by pre-incubation of rChIL-22 with rChIL22BP. Together, this study indicates an important role of ChIL-22 on epithelial cells and hepatocytes during inflammation.
authors.affiliation: Avian Immunobiology Laboratory, Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, USA.
authors.forename: Sungwon
authors.initials: S
authors.lastname: Kim
authors.forename: Laura
authors.initials: L
authors.lastname: Faris
authors.forename: Chasity M
authors.initials: CM
authors.lastname: Cox
authors.forename: Lindsay H
authors.initials: LH
authors.lastname: Sumners
authors.forename: Mark C
authors.initials: MC
authors.lastname: Jenkins
authors.forename: Ray H
authors.initials: RH
authors.lastname: Fetterer
authors.forename: Kate B
authors.initials: KB
authors.lastname: Miska
authors.forename: Rami A
authors.initials: RA
authors.lastname: Dalloul
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D049109:Cell Proliferation; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology; D004847:Epithelial Cells / Q000276:immunology*; D005347:Fibroblasts; D022781:Hepatocytes / Q000276:immunology*; D007249:Inflammation / Q000276:immunology*; D007378:Interleukins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008969:Molecular Sequence Data; D018123:Receptors, Interleukin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution; D000097043:Interleukin-22
chemical_list: D007378:Interleukins; D018123:Receptors, Interleukin; D011994:Recombinant Proteins; D000097043:Interleukin-22; C415369:interleukin-22 receptor