title: High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems.
abstract: Glycosylation research has gained significant attention in several research fields including immunology, protein production, and biomarker discovery. However, complex and time-consuming protocols are often necessary to obtain suitable samples for analysis. We here describe a short and robust assay to prepare 8-aminopyrene-1,3,6-trisulphonic acid-labeled N-glycans from serum samples. It only involves the subsequent addition of reagents and incubation in a PCR thermocycler. Moreover, this assay allows the detection of these glycans, which are only present in minute amounts in serum, on high-throughput microfluidics CE platforms including the MCE-202 MultiNA, 2100 Bioanalyzer, and eGene system. Using this clinical glycomics assay, we could reliably measure GlycoHepatoTest, a panel of biomarkers allowing the follow-up of chronic liver disease patients from the early stage onward.
authors.affiliation: Department for Molecular Biomedical Research, Unit for Molecular Glycobiology, VIB, Ghent, Belgium.
authors.forename: Dieter
authors.initials: D
authors.lastname: Vanderschaeghe
authors.forename: Andras
authors.initials: A
authors.lastname: Guttman
authors.forename: Nico
authors.initials: N
authors.lastname: Callewaert
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D054794:Glycomics / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D055442:Metabolome; D044085:Microfluidics / Q000379:methods*; D011134:Polysaccharides / Q000097:blood* / Q000737:chemistry; D011721:Pyrenes / Q000737:chemistry
chemical_list: C098210:1-aminopyrene-3,6,8-trisulfonic acid; D011134:Polysaccharides; D011721:Pyrenes