title: Chemical and antifungal investigations of six Lippia species (Verbenaceae) from Brazil.
abstract: The Lippia genus is used in ethnobotany as food, beverages, seasoning and antiseptic remedies, among others. The chemical compositions of fifteen extracts of six Lippia species were investigated comparatively by HPLC-PDA. To avoid data replication of previous works on this genus, Lippia lupulina Cham. root ethanol extract was selected for isolation procedures based on Principal Component Analyses (PCA) of such data. Seven compounds previously unreported in this genus were isolated from this extract (a triterpene, two furanonaphtoquinones, a furanochromone, an isoflavone, a stilbene and an iridoid). The activities of extracts, fractions and pure compounds towards Candida albicans, Candida krusei, Candida parapsilosis and Cryptococcus neoformans were investigated. Two fractions from the extract of Lippia salviaefolia leaves showed marked inhibition of fungal growth, in addition to verbascoside and asebogenin, which showed MICs lower than 15.6μg/ml and may be promising leads for the development of new antifungal agents, especially against C. neoformans.
authors.affiliation: NuBBE, Institute of Chemistry, São Paulo State University, Araraquara, CP 355, CEP 14801-970, SP, Brazil.
authors.forename: Cristiano Soleo
authors.initials: CS
authors.lastname: Funari
authors.forename: Fernanda Patrícia
authors.initials: FP
authors.lastname: Gullo
authors.forename: Assunta
authors.initials: A
authors.lastname: Napolitano
authors.forename: Renato Lajarim
authors.initials: RL
authors.lastname: Carneiro
authors.forename: Maria José Soares
authors.initials: MJ
authors.lastname: Mendes-Giannini
authors.forename: Ana Marisa
authors.initials: AM
authors.lastname: Fusco-Almeida
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.forename: Dulce Helena Siqueira
authors.initials: DH
authors.lastname: Silva
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001938:Brazil; D005658:Fungi / Q000187:drug effects; D032411:Lippia / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts