title: A conserved regulatory element located far downstream of the gls locus modulates gls expression through chromatin loop formation during myogenesis.
abstract: Chromatin loops formed between distant regulatory elements and promoters modulate gene expression. We identified a novel distant regulatory element located approximately 120kb downstream of the gls promoter, and examined its regulatory relevance to gls gene expression in C2C12 cells by a chromosome conformation capture assay. The distant element physically interacted with the gls promoter in myoblasts but not in myotubes. Semiquantitative analysis by real-time PCR showed more abundant gls transcripts in myoblasts than in myotubes. These findings suggest that this distant element differentially regulates gls gene expression through dynamic formation and abrogation of a chromatin loop during myogenesis.
authors.affiliation: Department of Anatomy and Cell Biology, Research Institute of Pharmaceutical Science, Faculty of Pharmacy, Musashino University, Nishitokyo, Tokyo, Japan.
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yuasa
authors.forename: Shin'ichi
authors.initials: S
authors.lastname: Takeda
authors.forename: Takao
authors.initials: T
authors.lastname: Hijikata
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D042002:Chromatin Assembly and Disassembly / Q000235:genetics*; D017124:Conserved Sequence; D003470:Culture Media; D004247:DNA / Q000235:genetics; D005972:Glutaminase / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D024510:Muscle Development / Q000235:genetics*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D032446:Myoblasts / Q000378:metabolism; D011401:Promoter Regions, Genetic; D050436:Regulatory Elements, Transcriptional; D012689:Sequence Homology, Nucleic Acid
chemical_list: D003470:Culture Media; D004247:DNA; D005972:Glutaminase