title: Simultaneous determination of eleven preservatives in cosmetics by micellar electrokinetic chromatography.
abstract: A new method for the simultaneous quantitation of 11 preservatives-imidazolidinyl urea, benzyl alcohol, dehydroacetic acid, sorbic acid, phenoxyethanol, benzoic acid, salicylic acid, and four parabens (methyl, ethyl, propyl, and butyl)-in cosmetics by micellar electrokinetic capillary chromatography was established and validated. The separation was performed using an uncoated fused-silica capillary (50 pm id x 60.2 cm, effective length 50 cm) with a running buffer consisting of 15 mmol/L sodium tetraborate, 60 mmol/L boric acid, and 100 mmol/L sodium dodecyl sulfate. A 1:10 dilution of the running buffer was used as the sample buffer to extract the cosmetic samples. The key factors, such as the concentration and pH of the running and sample buffers, which influence quantitative analysis of the above 11 preservatives in cosmetic samples, were investigated in detail. The linear ranges of the calibration curves for imidazolidinyl urea and the other 10 preservatives were 50-1000 and 10-200 mg/L, respectively. The correlation coefficients of the standard curves were all higher than 0.999. The recoveries at the concentrations studied ranged from 93.0 to 102.7%. RSDs were all less than 5%. The new method with simple sample pretreatment met the needs for routine analysis of the 11 preservatives in cosmetics.
authors.affiliation: Beijing Center for Disease Control and Prevention, Beijing 100013, People's Republic of China. ping_wang@tom.com
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Ding
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Yang
mesh_terms: D019817:Benzoic Acid / Q000032:analysis; D019905:Benzyl Alcohol / Q000032:analysis; D002021:Buffers; D002138:Calibration; D002623:Chemistry Techniques, Analytical; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D003358:Cosmetics / Q000032:analysis*; D005026:Ethylene Glycols / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D010226:Parabens / Q000032:analysis; D011310:Preservatives, Pharmaceutical / Q000032:analysis*; D011753:Pyrones / Q000032:analysis; D012015:Reference Standards; D015203:Reproducibility of Results; D020156:Salicylic Acid / Q000032:analysis; D013011:Sorbic Acid / Q000032:analysis; D014508:Urea / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D002021:Buffers; D003358:Cosmetics; D005026:Ethylene Glycols; D008823:Micelles; D010226:Parabens; D011310:Preservatives, Pharmaceutical; D011753:Pyrones; C027493:dehydroacetic acid; D019817:Benzoic Acid; D014508:Urea; C005398:phenoxyethanol; D019905:Benzyl Alcohol; C006048:imidazolidinyl urea; D020156:Salicylic Acid; D013011:Sorbic Acid