title: The nonsteroidal antiinflammatory drug diclofenac potentiates the in vivo activity of caspofungin against Candida albicans biofilms.
abstract: In this study, we demonstrated that in vitro Candida albicans biofilms grown in the presence of diclofenac showed increased susceptibility to caspofungin. These findings were further confirmed using a catheter-associated biofilm model in rats. C. albicans-inoculated catheters retrieved from rats that were treated with both diclofenac and caspofungin contained significantly fewer biofilm cells and showed no visible biofilms inside the catheter lumens, as documented by scanning electron microscopy, as compared to catheters retrieved from rats receiving only caspofungin or diclofenac. This report indicates that diclofenac could be useful in combination therapy with caspofungin to treat C. albicans biofilm-associated infections.
authors.affiliation: Center of Microbial and Plant Genetics, KU Leuven, Heverlee, Belgium.
authors.forename: Anna
authors.initials: A
authors.lastname: Bink
authors.forename: Soňa
authors.initials: S
authors.lastname: Kucharíková
authors.forename: Bram
authors.initials: B
authors.lastname: Neirinck
authors.forename: Jef
authors.initials: J
authors.lastname: Vleugels
authors.forename: Patrick
authors.initials: P
authors.lastname: Van Dijck
authors.forename: Bruno P A
authors.initials: BP
authors.lastname: Cammue
authors.forename: Karin
authors.initials: K
authors.lastname: Thevissen
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects*; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology; D000077336:Caspofungin; D057785:Catheters / Q000382:microbiology; D004008:Diclofenac / Q000008:administration & dosage / Q000494:pharmacology*; D025141:Drug Resistance, Fungal; D004357:Drug Synergism; D054714:Echinocandins / Q000008:administration & dosage / Q000494:pharmacology*; D055666:Lipopeptides; D051381:Rats
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000935:Antifungal Agents; D054714:Echinocandins; D055666:Lipopeptides; D004008:Diclofenac; D000077336:Caspofungin