title: Overexpression and clinical significance of carcinoembryonic antigen-related cell adhesion molecule 6 in colorectal cancer.
abstract: BACKGROUND
  Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) inhibits anoikis and affects the malignant phenotype of cancer cells. In this study, we analyzed CEACAM6 as a gene that is highly upregulated in colon cancer tissues, and examined the assertion that CEACAM6 might be a suitable candidate tumor marker for the diagnosis of colon cancer.
  
  
  METHODS
  CEACAM6 gene expression in human colon tissues was performed by tissue microarray and analyzed using RT-PCR (each of normal and tumor tissue, n=40) and immunohistochemical and clinicopathological (colon cancer patients, n=143) analyses.
  
  
  RESULTS
  CEACAM6 transcriptional and translational levels were significantly upregulated in human tumor tissues compared to non-tumor regions, and clinicopathological analysis revealed a significant correlation between CEACAM6 protein expression and Dukes' stage (p<0.001). High expression levels of CEACAM6 were significantly associated with lower overall survival (p<0.001) and shorter recurrence-free survival (p<0.001). We demonstrated that knockdown of CEACAM6 with CEACAM6-specific small interfering RNA in colorectal cancer cells attenuated invasivity (35%); conversely, the overexpression of CEACAM6 increased invasiveness.
  
  
  CONCLUSIONS
  CEACAM6 is significantly upregulated in colon cancer tissues and is closely associated with poor prognosis, indicating that CEACAM6 might be used as a tumor biomarker and a potential therapeutic target for colon cancer.
authors.affiliation: Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Kwang Soo
authors.initials: KS
authors.lastname: Kim
authors.forename: Jong-Tae
authors.initials: JT
authors.lastname: Kim
authors.forename: Seon-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Min Ah
authors.initials: MA
authors.lastname: Kang
authors.forename: In Seong
authors.initials: IS
authors.lastname: Choe
authors.forename: Yun Hee
authors.initials: YH
authors.lastname: Kang
authors.forename: Seon-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Young Il
authors.initials: YI
authors.lastname: Yeom
authors.forename: Young-Ha
authors.initials: YH
authors.lastname: Lee
authors.forename: Joo Heon
authors.initials: JH
authors.lastname: Kim
authors.forename: Kyo Hyun
authors.initials: KH
authors.lastname: Kim
authors.forename: Chang Nam
authors.initials: CN
authors.lastname: Kim
authors.forename: Jong Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Myoung-Soo
authors.initials: MS
authors.lastname: Nam
authors.forename: Hee Gu
authors.initials: HG
authors.lastname: Lee
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000037:antagonists & inhibitors / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors / Q000235:genetics*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D005260:Female; D058851:GPI-Linked Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016019:Survival Analysis
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C504117:CEACAM6 protein, human; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D034741:RNA, Small Interfering