title: Cytoprotective properties of traditional Chinese medicinal herbal extracts in hydrogen peroxide challenged human U373 astroglia cells.
abstract: Age is the leading risk factor for many of the most prevalent and devastating diseases including neurodegenerative diseases. A number of herbal medicines have been used for centuries to ameliorate the deleterious effects of ageing-related diseases and increase longevity. Oxidative stress is believed to play a role in normal ageing as well as in neurodegenerative processes. Since many of the constituents of herbal extracts are known antioxidants, it is believed that restoring oxidative balance may be one of the underlying mechanisms by which medicinal herbs can protect against ageing and cognitive decline. Based on the premise that astrocytes are key modulators in the progression of oxidative stress associated neurodegenerative diseases, 13 herbal extracts purported to possess anti-ageing properties were tested for their ability to protect U373 human astrocytes from hydrogen peroxide induced cell death. To determine the contribution of antioxidant activity to the cytoprotective ability of extracts, total phenol content and radical scavenging capacities of extracts were examined. Polygonum multiflorum, amongst others, was identified as possessing potent antioxidant and cytoprotective properties. Not surprisingly, total phenol content of extracts was strongly correlated with antioxidant capacity. Interestingly, when total phenol content and radical scavenging capacities of extracts were compared to the cytoprotective properties of extracts, only moderately strong correlations were observed. This finding suggests the involvement of multiple protective mechanisms in the beneficial effects of these medicinal herbs.
authors.affiliation: Dept. of Pharmacology, School of Medicine, University of Western Sydney, Campbelltown, NSW, Australia.
authors.forename: Megan L
authors.initials: ML
authors.lastname: Steele
authors.forename: John
authors.initials: J
authors.lastname: Truong
authors.forename: Suresh
authors.initials: S
authors.lastname: Govindaraghavan
authors.forename: Lezanne
authors.initials: L
authors.lastname: Ooi
authors.forename: Nikolaus J
authors.initials: NJ
authors.lastname: Sucher
authors.forename: Gerald
authors.initials: G
authors.lastname: Münch
mesh_terms: D001253:Astrocytes / Q000166:cytology / Q000187:drug effects*; D002460:Cell Line; D004365:Drugs, Chinese Herbal; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D010636:Phenols; D010936:Plant Extracts; D006861:Hydrogen Peroxide