title: ATP-dependent export of neutral amino acids by vacuolar membrane vesicles of Saccharomyces cerevisiae.
abstract: Amino acid analysis of Saccharomyces cerevisiae cells indicated that neutral amino acids such as glycine and alanine were probably excluded from the vacuoles, and that vacuolar H(+)-ATPase (V-ATPase) was involved in the vacuolar compartmentalization of these amino acids. We found that vacuolar membrane vesicles export neutral amino acids in an ATP-dependent manner. This is important in identifying vacuolar transporters for neutral amino acids.
authors.affiliation: Laboratory of Molecular Physiology and Genetics, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Ishimoto
authors.forename: Naoko
authors.initials: N
authors.lastname: Sugimoto
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sekito
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kawano-Kawada
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kakinuma
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D021542:Amino Acids, Neutral / Q000378:metabolism*; D001692:Biological Transport; D007425:Intracellular Membranes / Q000737:chemistry; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D022161:Transport Vesicles / Q000737:chemistry / Q000378:metabolism*; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism*; D014617:Vacuoles / Q000737:chemistry
chemical_list: D021542:Amino Acids, Neutral; D029701:Saccharomyces cerevisiae Proteins; D000255:Adenosine Triphosphate; D025262:Vacuolar Proton-Translocating ATPases