title: Immunosensor based on the ZnO nanorod networks for the detection of H1N1 swine influenza virus.
abstract: This paper presents an immunosensor fabricated on patterned zinc oxide nanorod networks (ZNNs) for detecting the H1N1 swine influenza virus (H1N1 SIV). Nanostructured ZnO with a high isoelectric point (IEP, approximately 9.5) possesses good absorbability for proteins with low IEPs. Hydrothermally grown ZNNs were fabricated on a patterned Au electrode (0.02 cm2) through a lift-off process. To detect the H1N1 SIV, the sandwich enzyme-linked immunosorbent assay (ELISA) method was employed in the immunosensor. The immunosensor was evaluated in an acetate buffer solution containing 3,3',5,5'-tetramethylbenzidine (TMB) via cyclic voltammetry at various H1N1 SIV concentrations (1 pg/mL-5 ng/mL). The measurement results of the fabricated immunosensor showed that the reduction currents of TMB at 0.25 V logarithmically increased from 259.37 to 577.98 nA as the H1N1 SIV concentration changed from 1 pg/mL to 5 ng/mL. An H1N1 SIV immunosensor, based on the patterned ZNNs, was successfully realized for detecting 1 pg/mL-5 ng/mL H1N1 SIV concentrations, with a detection limit of 1 pg/mL for H1N1 SIV.
authors.affiliation: School of Electrical Engineering, Korea University Anam-dong 5-Ga, Seongbuk-Gu, Seoul 136-713, Republic of Korea.
authors.forename: Yunseok
authors.initials: Y
authors.lastname: Jang
authors.forename: Jungil
authors.initials: J
authors.lastname: Park
authors.forename: Youngmi Kim
authors.initials: YK
authors.lastname: Pak
authors.forename: James Jungho
authors.initials: JJ
authors.lastname: Pak
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D053118:Influenza A Virus, H1N1 Subtype / Q000302:isolation & purification*; D036103:Nanotechnology / Q000295:instrumentation; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D013552:Swine; D019562:Viral Load / Q000295:instrumentation*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D015034:Zinc Oxide