title: Detergent selection for enhanced extraction of membrane proteins.
abstract: Generating stable conditions for membrane proteins after extraction from their lipid bilayer environment is essential for subsequent characterization. Detergents are the most widely used means to obtain this stable environment; however, different types of membrane proteins have been found to require detergents with varying properties for optimal extraction efficiency and stability after extraction. The extraction profiles of several detergent types have been examined for membranes isolated from bacteria and yeast, and for a set of recombinant target proteins. The extraction efficiencies of these detergents increase at higher concentrations, and were shown to correlate with their respective CMC values. Two alkyl sugar detergents, octyl-β-d-glucoside (OG) and 5-cyclohexyl-1-pentyl-β-d-maltoside (Cymal-5), and a zwitterionic surfactant, N-decylphosphocholine (Fos-choline-10), were generally effective in the extraction of a broad range of membrane proteins. However, certain detergents were more effective than others in the extraction of specific classes of integral membrane proteins, offering guidelines for initial detergent selection. The differences in extraction efficiencies among this small set of detergents supports the value of detergent screening and optimization to increase the yields of targeted membrane proteins.
authors.affiliation: Department of Chemistry, University of Toledo, 2801 W. Bancroft St., Toledo, OH 43606, United States.
authors.forename: Buenafe T
authors.initials: BT
authors.lastname: Arachea
authors.forename: Zhen
authors.initials: Z
authors.lastname: Sun
authors.forename: Nina
authors.initials: N
authors.lastname: Potente
authors.forename: Radhika
authors.initials: R
authors.lastname: Malik
authors.forename: Dragan
authors.initials: D
authors.lastname: Isailovic
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Viola
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002462:Cell Membrane / Q000737:chemistry; D003902:Detergents / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D010767:Phosphorylcholine / Q000031:analogs & derivatives / Q000737:chemistry; D010843:Pichia / Q000737:chemistry; D011489:Protein Denaturation; D055550:Protein Stability; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D005656:Fungal Proteins; D005960:Glucosides; D008565:Membrane Proteins; D013501:Surface-Active Agents; C400643:cyclohexyl-pentyl-maltoside; D010767:Phosphorylcholine; C018619:octyl-beta-D-glucoside; C031167:N-decylphosphorylcholine