title: A novel alkaline esterase from Sporosarcina sp. nov. strain eSP04 catalyzing the hydrolysis of a wide variety of aryl-carboxylic acid esters.
abstract: A novel esterase showing activity specific for esters of aryl-carboxylic acids was discovered in Sporosarcina sp. nov., which was identified by the 16S rDNA sequencing method in addition to morphological and physiological analyses. The aryl-carboxylesterase (named EstAC) was purified 780-fold from crude cell extracts by a 5-step procedure. EstAC was characterized as a monomeric protein with a molecular weight of 43,000, an optimum pH of around 9.0, and an optimum temperature of 40 °C. The pH optimum and the effects of inhibitors together with an internal amino acid sequence suggested that EstAC is a member of family VIII esterases. EstAC was found to be highly active on a wide variety of substrates such as alkyl benzoates, alkyl phenylacetates, ethyl α- or β-substituted phenylpropionates, dialkyl terephthalates, dimethyl isophthalate, and ethylene glycol dibenzoate. However, monomethyl terephthalate was not hydrolyzed. It was suggested that EstAC had 4-hydroxybenzoyl and cinnamoyl esterase activities as well.
authors.affiliation: Department of Materials Science, University of Shiga Prefecture, Hassaka, Hikone 522-8533, Japan. takehara@mat.usp.ac.jp
authors.forename: Munenori
authors.initials: M
authors.lastname: Takehara
authors.forename: Kaori
authors.initials: K
authors.lastname: Kinoshita
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyamoto
authors.forename: Hideo
authors.initials: H
authors.lastname: Hirohara
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D055162:Biocatalysis; D043182:Carboxylesterase / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D002264:Carboxylic Acids / Q000737:chemistry / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000235:genetics; D004952:Esters; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008970:Molecular Weight; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058644:Sporosarcina / Q000737:chemistry / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002264:Carboxylic Acids; D004275:DNA, Ribosomal; D004952:Esters; D012336:RNA, Ribosomal, 16S; D043182:Carboxylesterase