title: Microwave-assisted one-step extraction-derivatization for rapid analysis of fatty acids profile in herbal medicine by gas chromatography-mass spectrometry.
abstract: A rapid and practical microwave-assisted one-step extraction-derivatization (MAED) method was developed for gas chromatography-mass spectrometry analysis of fatty acids profile in herbal medicine. Several critical experimental parameters for MAED, including reaction temperature, microwave power and the amount of derivatization reagent (methanol), were optimized with response surface methodology. The results showed that the chromatographic peak areas of total fatty acids and total unsaturated fatty acids content obtained with MAED were markedly higher than those obtained by the conventional Soxhlet or microwave extraction and then derivatization method. The investigation of kinetics and thermodynamics of the derivatization reaction revealed that microwave assistance could reduce activation energy and increase the Arrhenius pre-exponential factor. The MAED method simplified the sample preparation procedure, shortened the reaction time, but improved the extraction and derivatization efficiency of lipids and reduced ingredient losses, especially for the oxidization and isomerization of unsaturated fatty acids. The simplicity, speed and practicality of this method indicates great potential for high throughput analysis of fatty acids in natural medicinal samples.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Rui-Lin
authors.initials: RL
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhao-Li
authors.initials: ZL
authors.lastname: Mou
authors.forename: Shuang-Li
authors.initials: SL
authors.lastname: Hao
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Zhang
mesh_terms: D002384:Catalysis; D005591:Chemical Fractionation / Q000379:methods*; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006878:Hydroxides / Q000737:chemistry; D007700:Kinetics; D008872:Microwaves; D031343:Perilla / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry; D012639:Seeds / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D005227:Fatty Acids; D006878:Hydroxides; D010936:Plant Extracts; D017680:Potassium Compounds; C029943:potassium hydroxide