title: Development of a sensitive enzyme-linked immunosorbent assay for the detection of fumonisin B₁ in maize.
abstract: Fumonisin B₁ (FB₁) is a mycotoxin, mainly produced by Fusarium fungi and present in food and feed. It causes harmful effects on human and animal health. Therefore, it is necessary to develop sensitive and reliable screening methods. In this study, a highly sensitive monoclonal antibody (MAb) against FB₁, clone 2D7, was produced, and the 50% inhibition concentration (IC₅₀) of the MAb was 2.2 ng/mL in buffer. The MAb showed high cross-reactivity with fumonisin B₂ (FB₂), and negligible cross-reactivity with other mycotoxins. A sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) based on this MAb was developed for the determination of FB₁ in maize. In spiked samples (100, 200 and 500 μg/kg), the average recoveries ranged from 78 ± 11 to 107 ± 4%, and the coefficient of variation ranged from 3 to 15%. The limit of detection of the icELISA was 5.4 μg/kg. This method was compared to liquid chromatography tandem mass spectrometry (LC-MS/MS) using naturally contaminated samples, and the correlation coefficient was above 0.82. These results show the reliability of the icELISA method for the determination of FB₁ in maize.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Sheng
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Jiang
authors.forename: Sarah
authors.initials: S
authors.lastname: De Saeger
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.forename: Suxia
authors.initials: S
authors.lastname: Zhang
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D037341:Fumonisins / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009183:Mycotoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D003313:Zea mays / Q000737:chemistry*
chemical_list: D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1