title: Oleic acid mitigates TNF-α-induced oxidative stress in rat cardiomyocytes.
abstract: Some of the effects of tumor necrosis factor alpha (TNF-α) are suggested to be mediated by oxidative stress. It has also been reported that dietary supplements of olive oil result in a reduction in LDL, oxidative stress, and blood pressure and these effects are attributed to oleic acid (OA)-a major component of olive oil. The objective of this study was to examine the beneficial effects of OA against TNF-α-induced oxidative stress and cardiomyocytes injury. Isolated cardiomyocytes from adult rat hearts were treated as follows: (A) control; (B) OA (50 μM); (C) TNF-α (10 ng/ml); and (D) TNF-α + OA. After 4 h of the treatment, cells were assessed for oxidative stress, cellular damage, viability, and apoptosis. Cardiomyocytes treated with TNF-α showed a significant increase (P < 0.05) in reactive oxygen species, decrease in the viability of cells, and increase in creatine kinase release. All these TNF-α-induced changes were prevented by OA. TNF-α also caused a significant increase in the expression of apoptotic proteins Bax, Caspase 3 and PARP cleavage, Bnip3, and TGF-β , whereas OA modulated these changes. It is suggested that TNF-α induced oxidative stress mediates cardiomyocyte cell damage which is prevented by OA.
authors.affiliation: Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, Room R3022, 351 Tache Ave, Winnipeg, MB, R2H 2A6, Canada.
authors.forename: Abd Al Rahman
authors.initials: AA
authors.lastname: Al-Shudiefat
authors.forename: Anita K
authors.initials: AK
authors.lastname: Sharma
authors.forename: Ashim K
authors.initials: AK
authors.lastname: Bagchi
authors.forename: Sanjiv
authors.initials: S
authors.lastname: Dhingra
authors.forename: Pawan K
authors.initials: PK
authors.lastname: Singal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D003402:Creatine Kinase / Q000378:metabolism; D006331:Heart Diseases / Q000188:drug therapy; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D019301:Oleic Acid / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology / Q000502:physiology*
chemical_list: D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D014409:Tumor Necrosis Factor-alpha; D019301:Oleic Acid; D006861:Hydrogen Peroxide; D003402:Creatine Kinase