title: Hyaluronic acid-functionalized mesoporous silica nanoparticles for efficient photodynamic therapy of cancer cells.
abstract: Mesoporous silica nanoparticles (MSN) for photodynamic therapy (PDT) were coated with poly-(L-lysine) and hyaluronic acid (HA) by using the layer-by-layer method. HA is able to target cancer cells over-expressing the corresponding CD44 receptor. MSN functionalized with HA (MSN-HA) were more efficient than MSN without the targeting moiety when PDT was performed at low fluence (14 Jcm(-2)) and low dosage of MSN (20 μgmL(-1)) on HCT 116 colorectal cancer cells, known to over-express the CD44 receptor. Incubation of HCT-116 cancer cells with an excess of HA impaired the PDT effect with MSN-HA thus demonstrating that an active endocytosis mechanism was involved in the uptake of MSN-HA by these cells.
authors.affiliation: Institut des Biomolécules Max Mousseron UMR 5247 CNRS, Université Montpellier 1, Université Montpellier 2 - Faculté de Pharmacie, 15 Avenue Charles Flahault, 34093 Montpellier Cedex 05, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Gary-Bobo
authors.forename: David
authors.initials: D
authors.lastname: Brevet
authors.forename: Nadia
authors.initials: N
authors.lastname: Benkirane-Jessel
authors.forename: Laurence
authors.initials: L
authors.lastname: Raehm
authors.forename: Philippe
authors.initials: P
authors.lastname: Maillard
authors.forename: Marcel
authors.initials: M
authors.lastname: Garcia
authors.forename: Jean-Olivier
authors.initials: JO
authors.lastname: Durand
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry*; D010778:Photochemotherapy / Q000379:methods*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: D053769:Nanocapsules; D012822:Silicon Dioxide; D006820:Hyaluronic Acid