title: Application of three phase hollow fiber based liquid phase microextraction (HF-LPME) for the simultaneous HPLC determination of phenol substituting compounds (alkyl-, chloro- and nitrophenols).
abstract: This work proposes for the first time the use of a three phase hollow fiber liquid phase microextraction (HF-LPME) procedure for the simultaneous extraction, and the later HPLC determination, of some phenol substituting compounds (alkyl-, chloro- and nitrophenols) that are considered as highly toxic compounds and/or endocrine disrupting ones. The substances studied include four chlorophenols (CPs): 2,4-dichlorophenol (2,4-DCP), 2,5-dichlorophenol (2,5-DCP), 2,6-dichlorophenol (2,6-DCP) and pentachlorophenol (PCP), three nitrophenols (NPs): 2,4-dinitrophenol (2,4-DNP), 2,5-dinitrophenol (2,5-DNP) and 2,6-dinitriphenol (2,6-DNP) and two alkylphenols (APs): tert butylphenol (TBP) and sec butylphenol (SBP). The extraction was carried out through a dihexyl ether liquid membrane supported on an Accurel(®) Q3/2 polypropylene hollow fiber. Optimum pH for donor and acceptor phases and extraction time were established. The enrichment (preconcentration) factors obtained were between 30 and 700 that allows detection limits between 140 and 290 pg mL(-1). The method was successfully applied to the determination of the compounds in environmental water samples, including urban wastewaters.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, University of Seville, 41012 Seville, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Villar-Navarro
authors.forename: María
authors.initials: M
authors.lastname: Ramos-Payán
authors.forename: Juan Luis
authors.initials: JL
authors.lastname: Pérez-Bernal
authors.forename: Rut
authors.initials: R
authors.lastname: Fernández-Torres
authors.forename: Manuel
authors.initials: M
authors.lastname: Callejón-Mochón
authors.forename: Miguel
authors.initials: M
authors.lastname: Ángel Bello-López
mesh_terms: D002733:Chlorophenols / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D059627:Liquid Phase Microextraction / Q000379:methods*; D008567:Membranes, Artificial; D009596:Nitrophenols / Q000032:analysis / Q000302:isolation & purification; D019800:Phenol / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D062065:Wastewater / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D002733:Chlorophenols; D008567:Membranes, Artificial; D009596:Nitrophenols; D019800:Phenol; D012997:Solvents; D062065:Wastewater; D014867:Water