title: First molecular identification and characterization of classical swine fever virus isolates from Nepal.
abstract: Classical swine fever (CSF) is a major constraint to pig production worldwide, and in many developing countries, the epidemiological status is unknown. Here, for the first time, molecular identification and characterization of CSFV isolates from two recent outbreaks in Nepal are presented. Analysis of full-length E2-encoding sequences revealed that these isolates belonged to CSFV subgenotype 2.2 and had highest genetic similarity to isolates from India. Hence, for CSFV, Nepal and India should be regarded as one epidemiological unit. Both Nepalese isolates exhibited significant sequence differences, excluding a direct epidemiological connection and suggesting that CSFV is endemic in that country.
authors.affiliation: Department of Infectious Diseases, EU and OIE Reference Laboratory for Classical Swine Fever, Institute of Virology, University of Veterinary Medicine, Buenteweg 17, 30559 Hannover, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Postel
authors.forename: Vijay C
authors.initials: VC
authors.lastname: Jha
authors.forename: Stefanie
authors.initials: S
authors.lastname: Schmeiser
authors.forename: Paul
authors.initials: P
authors.lastname: Becher
mesh_terms: D000818:Animals; D006691:Classical Swine Fever / Q000453:epidemiology / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D014644:Genetic Variation; D007194:India; D008969:Molecular Sequence Data; D009390:Nepal / Q000453:epidemiology; D010802:Phylogeny; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C087086:glycoprotein E2, classical swine fever virus