title: Dual functions of Arabidopsis sulfiredoxin: acting as a redox-dependent sulfinic acid reductase and as a redox-independent nuclease enzyme.
abstract: Based on the fact that the amino acid sequence of sulfiredoxin (Srx), already known as a redox-dependent sulfinic acid reductase, showed a high sequence homology with that of ParB, a nuclease enzyme, we examined the nucleic acid binding and hydrolyzing activity of the recombinant Srx in Arabidopsis (AtSrx). We found that AtSrx functions as a nuclease enzyme that can use single-stranded and double-stranded DNAs as substrates. The nuclease activity was enhanced by divalent cations. Particularly, by point-mutating the active site of sulfinate reductase, Cys (72) to Ser (AtSrx-C72S), we demonstrate that the active site of the reductase function of AtSrx is not involved in its nuclease function.
authors.affiliation: Division of Applied Life Science (BK21 Program) and PMBBRC, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Yong Hun
authors.initials: YH
authors.lastname: Chi
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.forename: In Jung
authors.initials: IJ
authors.lastname: Jung
authors.forename: Mi Rim
authors.initials: MR
authors.lastname: Shin
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Jung
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Eun Seon
authors.initials: ES
authors.lastname: Lee
authors.forename: Punyakishore
authors.initials: P
authors.lastname: Maibam
authors.forename: Kang-San
authors.initials: KS
authors.lastname: Kim
authors.forename: Joung Hun
authors.initials: JH
authors.lastname: Park
authors.forename: Min Ji
authors.initials: MJ
authors.lastname: Kim
authors.forename: Gwang Yong
authors.initials: GY
authors.lastname: Hwang
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D002413:Cations, Divalent / Q000494:pharmacology; D018744:DNA, Plant / Q000235:genetics; D003851:Deoxyribonucleases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013441:Sulfinic Acids / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D002413:Cations, Divalent; D018744:DNA, Plant; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D013441:Sulfinic Acids; D050862:Oxidoreductases Acting on Sulfur Group Donors; C520419:sulfiredoxin protein, Arabidopsis; D003851:Deoxyribonucleases