title: Reproducibility of optical coherence tomography measurements in children.
abstract: OBJECTIVE
  To determine the interobserver and intraobserver reproducibility of a Fourier-domain optical coherence tomography device (Cirrus HD OCT; Carl Zeiss Meditec, Dublin, California, USA) in normal pediatric eyes.
  
  
  METHODS
  Prospective cross-sectional study.
  
  
  METHODS
  One hundred healthy children were recruited prospectively and consecutively. Only 1 randomly chosen eye per subject was included in the study. The eye underwent 3 scans centered on the optic disc and another 3 scans centered on the macula that were acquired by a single operator. A fourth examination was performed by a second operator. Interobserver and intraobserver reproducibility were described by intraclass correlation coefficients (ICCs) and coefficients of variation (COVs).
  
  
  RESULTS
  The mean age was 9.15 years (range, 6.22 to 11.31 years; standard deviation, 1.05 years). Mean retinal nerve fiber layer thickness was 99.53 μm (standard deviation, 10.10 μm), and mean macular thickness was 282.91 μm (standard deviation, 11.83 μm). All the parameters evaluated were highly reproducible. Intraobserver COVs of the retinal nerve fiber layer measurements ranged from 2.24% to 5.52%, and the COV of macular thickness was 0.97%. The intraclass correlation coefficient was greater than 0.8 for all the parameters. The interobserver COV ranged from 2.23% to 5.18%, and the COV of macular thickness was 0.82%. In all the evaluated parameters, the intraclass correlation coefficient was more than 0.75. Repeatability was slightly better in children older than 10 years than in children younger than 9 years.
  
  
  CONCLUSIONS
  Retinal nerve fiber layer and macular measurements obtained by Fourier-domain optical coherence tomography showed good repeatability for healthy eyes in the pediatric population. Cirrus HD OCT examinations of the retina are reliable in children.
authors.affiliation: Department of Ophthalmology, Hospital Universitario Miguel Servet, Zaragoza, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: Altemir
authors.forename: Victoria
authors.initials: V
authors.lastname: Pueyo
authors.forename: Noemi
authors.initials: N
authors.lastname: Elía
authors.forename: Vicente
authors.initials: V
authors.lastname: Polo
authors.forename: Jose M
authors.initials: JM
authors.lastname: Larrosa
authors.forename: Daniel
authors.initials: D
authors.lastname: Oros
mesh_terms: D002648:Child; D003430:Cross-Sectional Studies; D003941:Diagnostic Techniques, Ophthalmological / Q000295:instrumentation*; D005260:Female; D005583:Fourier Analysis; D006801:Humans; D008266:Macula Lutea / Q000033:anatomy & histology*; D008297:Male; D009412:Nerve Fibers; D015588:Observer Variation; D009898:Optic Disk / Q000033:anatomy & histology*; D011446:Prospective Studies; D015203:Reproducibility of Results; D012165:Retinal Ganglion Cells / Q000166:cytology*; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000592:standards*; D014792:Visual Acuity / Q000502:physiology