title: [Expression of MCP-1 in the patients of chronic hepatitis B complicated with nonalcoholic fatty liver disease].
abstract: OBJECTIVE
  To investigate the expression level of monocyte chemoattractant protein-1 (MCP-1) in the liver of the patients with chronic hepatitis B (CHB) complicated with non-alcoholic fatty liver diseases (NAFLD).
  
  
  METHODS
  The study enrolled 21 CHB with NAFLD patients and 46 CHB without NAFLD patients as the controls. Real-time quantitative reverse transcriptase-polymerase chain reaction (QRT-PCR) and immunohistochemistry (IHC) were applied to detect the expression of MCP-1 at the mRNA and protein levels in the liver tissues,  respectively. Non-parametric Mann-Whitney test was used to analyze the difference between the CHB patients with and without NAFLD.
  
  
  RESULTS
  The mRNA relative expression level of MCP-1 in CHB+NAFLD group was 0.034 (0.024-0.058),  higher than that in the control group 0.016 (0.012-0.024). The  immunohistochemical score was 8.7±2.5 in CHB+NAFLD group and 6.2±3.5 in the control group. The difference in MCP-1 expression at the both protein and mRNA levels was significant statistically between the two groups (P<0.01).
  
  
  CONCLUSIONS
  MCP-1 expression level in the liver is higher in CHB with NAFLD patients than that in CHB without NAFLD patients.
authors.affiliation: Department of Clinical Laboratory, Xuzhou Central Hospital, Xuzhou, China.
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Shen
authors.forename: Yan-chao
authors.initials: YC
authors.lastname: Deng
authors.forename: Qing-mei
authors.initials: QM
authors.lastname: Wang
authors.forename: Quan-shuang
authors.initials: QS
authors.lastname: Li
authors.forename: Zhan
authors.initials: Z
authors.lastname: Xu
mesh_terms: D018932:Chemokine CCL2 / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D005234:Fatty Liver / Q000209:etiology*; D019694:Hepatitis B, Chronic / Q000150:complications* / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism; D065626:Non-alcoholic Fatty Liver Disease; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2