title: Seed oil triglyceride profiling of thirty-two hybrid grape varieties.
abstract: Triglyceride profile of seed oil samples from 32 hybrid grape varieties not studied before was investigated. A new method for the analysis of triacylglycerols (TAGs) has been developed based on the direct infusion in the electrospray ionization (ESI) source and employing tetrahydrofuran/methanol/water (85:10:5 v|v|v) as solvent; the formation of [M + Na](+) ions in high yield has been observed. TAGs were identified by ESI-tandem mass spectrometry analysis, and the matrix-assisted-laser-desorption-ionization and time-of-flight profile of samples was determined. Six were the principal TAGs identified in seed oil: trilinolein (LLL) was the most abundant (43%), followed by dilinoleoyl-oleoylglycerol (LOL, 23%), and dilinoleoyl-palmitoylglycerol (LPL, 15%). Compounds present in lower concentration were LSL and LOO (11%), LOP (6%), and LSP (2%). Compared with seed oils produced from V. Vinifera grapes, some significant differences in the relative abundances of TAGs were found, in particular hybrid grape seed oils showed higher LOL and lower LPL content, respectively. Among the samples studied, a particularly high content of LLL (rich in unsaturated fatty acids) was found in seed oils from two red varieties.
authors.affiliation: CRA-VIT, Viale XXVIII aprile 26, 31015, Conegliano, TV, Italy.
authors.forename: Fabiola
authors.initials: F
authors.lastname: De Marchi
authors.forename: Roberta
authors.initials: R
authors.lastname: Seraglia
authors.forename: Laura
authors.initials: L
authors.lastname: Molin
authors.forename: Pietro
authors.initials: P
authors.lastname: Traldi
authors.forename: Mirko
authors.initials: M
authors.lastname: De Rosso
authors.forename: Annarita
authors.initials: A
authors.lastname: Panighel
authors.forename: Antonio
authors.initials: A
authors.lastname: Dalla Vedova
authors.forename: Massimo
authors.initials: M
authors.lastname: Gardiman
authors.forename: Mirella
authors.initials: M
authors.lastname: Giust
authors.forename: Riccardo
authors.initials: R
authors.lastname: Flamini
mesh_terms: D000704:Analysis of Variance; D016000:Cluster Analysis; D010936:Plant Extracts / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014280:Triglycerides / Q000032:analysis*; D027843:Vitis / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D010938:Plant Oils; D014280:Triglycerides