title: Genetically encodable design of ligand "bundling" on the surface of nanoparticles.
abstract: Polyhistidine peptide dendrimer self-assembles on CdSe/ZnS quantum dots (QDs) with very high affinity and stability, a property ascribable to its multivalent geometry. Here we designed a fluorescent protein, GCN-mCherry, that exists as an oligomeric bundled structure in solution as well as on the surface to imitate the structure of a synthetic dendrimer. GCN-mCherry forms a very stable assembly with QDs, which can resist displacement by 500 mM imidazole and the dendrimer peptide, as measured by the Förster resonance energy transfer from QD to mCherry. Our work manifested a prominent stability enhancement of  protein-nanoparticle assembly through directional ligand-ligand interaction on the surface.
authors.affiliation: Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Lu
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Wang
authors.forename: Jianhao
authors.initials: J
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Shannon Wing-Ngor
authors.initials: SW
authors.lastname: Au
authors.forename: Jiang
authors.initials: J
authors.lastname: Xia
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry; D050091:Dendrimers / Q000138:chemical synthesis* / Q000737:chemistry; D006639:Histidine / Q000138:chemical synthesis* / Q000737:chemistry; D008024:Ligands; D008164:Luminescent Proteins / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D013499:Surface Properties; D017967:Zinc Compounds / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D050091:Dendrimers; D008024:Ligands; D008164:Luminescent Proteins; D010446:Peptide Fragments; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; C033223:polyhistidine; D006639:Histidine; C058667:cadmium selenide; C031238:zinc sulfide