title: Protective effect of intraperitoneal ozone application in experimental ovarian ischemia/reperfusion injury.
abstract: OBJECTIVE
  The current accepted management of ovarian torsion is ovary-sparing surgery. Ozone therapy is used to reduce ischemia/reperfusion (I/R) injury in several situations. An experimental study was designed to evaluate effect of ozone application in ovarian I/R injury.
  
  
  METHODS
  Three groups (n = 6) and 18 rats were included in the study. After anesthesia, right ovaries were fixed and removed at the end of 2 hours in sham group (SG). In torsion group (TG), right ovaries underwent 720° torsion in a counterclockwise direction. Ovaries were removed after 2 hours torsion and 2 hours reperfusion. In ozone group (OG), torsion was created by the same technique, and 95% oxygen plus 5% ozone gas mixture was given intraperitoneally (25 μg/mL, 0.5 mg/kg) 10 minutes before reperfusion. After 2 hours reperfusion, ovaries were removed. Histopathologic examination of ovarian and periovarian sections was performed for the presence of congestion (C), hemorrhage, interstitial edema (IE), and polymorphonuclear neutrophilic infiltrations. Tissue samples were analyzed for malondialdehyde, nitric oxide (NO), and total sulphidryl (t-SH) values. Results were compared between 3 groups.
  
  
  RESULTS
  At histopathologic examination, the TG have elevation in terms of ovarian C, polymorphonuclear neutrophilic infiltration, and periovarian IE when compared with SG (P < ,05). In OG, ovarian C and periovarian IE were reduced according to TG, whereas the increase was observed only in ovarian C compared with SG (P < .05). At biochemical evaluation of oxidative stress markers in SG and TG, there was no difference between them (P < .05). Malondialdehyde levels were significantly lower in OG than TG, whereas NO and t-SH values were higher (P < .05). Malondialdehyde levels were decreased in OG compared with SG (P < .05). However, no difference was observed in NO and t-SH levels (P > .05).
  
  
  CONCLUSIONS
  Intraperitoneal application of ozone creates a positive impact on histologic and biochemical markers on I/R injury owing to ovarian torsion. The ozone application can be developed to support efforts to protect ovary in ovarian torsion.
authors.affiliation: Department of Pediatric Surgery, Kırıkkale University, Medical Faculty, Kırıkkale 06570, Turkey. aslanmk@hotmail.com
authors.forename: Mustafa Kemal
authors.initials: MK
authors.lastname: Aslan
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Boybeyi
authors.forename: Mine F
authors.initials: MF
authors.lastname: Şenyücel
authors.forename: Şebnem
authors.initials: Ş
authors.lastname: Ayva
authors.forename: Ücler
authors.initials: Ü
authors.lastname: Kısa
authors.forename: Nurkan
authors.initials: N
authors.lastname: Aksoy
authors.forename: Tutku
authors.initials: T
authors.lastname: Soyer
authors.forename: Özkan
authors.initials: Ö
authors.lastname: Cesur
authors.forename: Murat
authors.initials: M
authors.lastname: Çakmak
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D004334:Drug Administration Schedule; D005260:Female; D007274:Injections, Intraperitoneal; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D010049:Ovarian Diseases / Q000150:complications*; D010053:Ovary / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D010126:Ozone / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D014102:Torsion Abnormality / Q000150:complications*; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D009569:Nitric Oxide; D008315:Malondialdehyde; D010126:Ozone