title: Antiaging effects of simvastatin on vascular endothelial cells.
abstract: The anti-inflammatory, antioxidative, and antiarteriosclerosis activities of simvastatin along with its protective effects on the endothelium suggest that it may also have antiaging effects. The aim of this study was to investigate the antiaging effects of simvastatin as well as its effects on sirtuin 1 (SIRT1) expression in endothelial cells. Aged rats and human umbilical vein endothelial cells were treated with simvastatin in the presence and absence of oxidized low-density lipoprotein (OX-LDL). Aortic β-galactosidase staining was undertaken to determine senescence, and SIRT1 protein expression was evaluated using Western blot analysis. After simvastatin therapy, arterial endothelial cell aging was significantly reduced, and SIRT1 expression was significantly increased. The OX-LDL significantly accelerated the senescence of umbilical vein endothelial cells and decreased SIRT1 expression. The OX-LDL-induced downregulation of SIRT1 was blocked by simvastatin. Simvastatin treatment also reduced umbilical vein endothelial cell aging and increased SIRT1 expression.
authors.affiliation: 1Department of Cardiovascular Diseases, Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Junping
authors.initials: J
authors.lastname: Lei
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.forename: Zhong
authors.initials: Z
authors.lastname: Ye
authors.forename: Jianbo
authors.initials: J
authors.lastname: Shi
authors.forename: Xing
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000369:Aged, 80 and over; D000818:Animals; D016922:Cellular Senescence / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D011897:Random Allocation; D051381:Rats; D019821:Simvastatin / Q000494:pharmacology*; D056564:Sirtuin 1 / Q000096:biosynthesis
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D019821:Simvastatin; C447939:SIRT1 protein, human; C488368:Sirt1 protein, rat; D056564:Sirtuin 1