title: Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation.
abstract: Cep135/Bld10 is a conserved centriolar protein required for the formation of the central cartwheel, an early intermediate in centriole assembly. Surprisingly, Cep135/Bld10 is not essential for centriole duplication in Drosophila, suggesting either that Cep135/Bld10 is not essential for cartwheel formation, or that the cartwheel is not essential for centriole assembly in flies. Using electron tomography and super-resolution microscopy we show that centrioles can form a cartwheel in the absence of Cep135/Bld10, but centriole width is increased and the cartwheel appears to disassemble over time. Using 3D structured illumination microscopy we show that Cep135/Bld10 is localized to a region between inner (SAS-6, Ana2) and outer (Asl, DSpd-2 and D-PLP) centriolar components, and the localization of all these component is subtly perturbed in the absence of Cep135/Bld10, although the ninefold symmetry of the centriole is maintained. Thus, in flies, Cep135/Bld10 is not essential for cartwheel assembly or for establishing the ninefold symmetry of centrioles; rather, it appears to stabilize the connection between inner and outer centriole components.
authors.affiliation: Sir William Dunn School of Pathology, Oxford University, South Parks Road, Oxford OX1 3RE,  UK.
authors.forename: Hélio
authors.initials: H
authors.lastname: Roque
authors.forename: Alan
authors.initials: A
authors.lastname: Wainman
authors.forename: Jennifer
authors.initials: J
authors.lastname: Richens
authors.forename: Kasia
authors.initials: K
authors.lastname: Kozyrska
authors.forename: Anna
authors.initials: A
authors.lastname: Franz
authors.forename: Jordan W
authors.initials: JW
authors.lastname: Raff
mesh_terms: D000818:Animals; D002502:Centrioles / Q000235:genetics / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D016253:Microscopy, Immunoelectron; D013090:Spermatocytes / Q000378:metabolism
chemical_list: C542499:CEP135 protein, Drosophila; D029721:Drosophila Proteins