title: Up-regulation of ski-interacting protein in rat brain cortex after traumatic brain injury.
abstract: SKIP (Ski-interacting protein), is part of nuclear regulatory complexes and interacts with factors involved in preinitiation, splicing and polyadenylation, potentiates the activity of important transcription factors, involved in an increasing number of signaling cascades. However, its distribution and function in the central nervous system remains poorly understood. In this study, western blot analysis, RT-PCR and immunohistochemistry showed a significant up-regulation of SKIP in ipsilateral peritrauma cortex compared with the sham group. Immunofluorescent labeling indicated that SKIP was localized striking in the neurons, but not astrocytes and oligodendrocytes; co-localization of SKIP and active caspase-3 and PCNA in the ipsilateral cortex. In addition, the expression patterns of active caspase-3 and PCNA were parallel with that of SKIP. Based on our data, we speculated that SKIP might play an important role in neuronal apoptosis following TBI; and might provide a basis for the further study on its role in cell cycle re-entry in traumatic brain injury.
authors.affiliation: Department of Neurosurgery, Hospital of Nantong University, Nantong, Jiangsu, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Mao
authors.forename: Huifei
authors.initials: H
authors.lastname: Zou
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Lanchun
authors.initials: L
authors.lastname: Ni
authors.forename: Kaifu
authors.initials: K
authors.lastname: Ke
authors.forename: Maohong
authors.initials: M
authors.lastname: Cao
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000235:genetics / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D008297:Male; D009474:Neurons / Q000378:metabolism; D051381:Rats; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C581694:SKIP protein, rat; D014157:Transcription Factors; D053148:Caspase 3