title: Development of an on-site measurement system for salivary stress-related substances based on microchip CE.
abstract: Secretory immunoglobulin A (sIgA) in saliva has been suggested to be a potential marker of chronic, long-term stress due to suppression of the immune system. A rapid point-of-care testing platform for stress measurement based on immunoassay and capillary electrophoresis (CE) separation was -developed using a poly(methyl methacrylate) (PMMA) chip and a laboratory-built microchip CE system. A method for the quantitative determination of sIgA in human saliva is introduced.
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Osaka, Japan. yoshihide-tanaka@aist.go.jp
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Nahoko
authors.initials: N
authors.lastname: Naruishi
mesh_terms: D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D007118:Immunoassay; D007071:Immunoglobulin A, Secretory / Q000378:metabolism*; D011312:Pressure; D012015:Reference Standards; D012463:Saliva / Q000378:metabolism*; D013315:Stress, Psychological / Q000175:diagnosis*
chemical_list: D007071:Immunoglobulin A, Secretory