title: The use of laser Doppler imaging as a predictor of burn depth and hypertrophic scar postburn injury.
abstract: Hypertrophic scarring (HTS) is a fibroproliferative disorder that commonly develops after severe burn injuries. Overexpression of transforming growth factor-β (TGF-β) by an increased number of fibrocytes has been associated with increased extracellular matrix molecule expression leading to HTS. The most widely accepted adjuvant to clinical assessment of burn depth is laser Doppler imaging (LDI) and may predict injury to the dermis that corresponds to cellular and molecular changes associated with HTS. A prospective, blinded, control trial was performed comparing LDI and clinical assessment for the decision to operate. Immunohistochemistry and real-time reverse transcription polymerase chain reaction was performed to determine whether there is a correlation between histological assessment of burn depth and LDI, and the presence of fibrocytes was detected using confocal microscopy. The positive predictive value for a burn requiring a graft was calculated to be >90%. Immunohistochemistry on biopsy samples revealed an increased expression of TGF-β, connective tissue growth factor, heat shock protein 47, and collagen type I in deep burn wounds compared to superficial burns. Using the fibrocyte-specific markers procollagen type I and lymphocyte-specific protein-1, there was an increased number of fibrocytes in deep burn areas compared to superficial burn. In deep burn injuries, increased infiltration of fibrocytes occurs leading to an overexpression of TGF-β1 and connective tissue growth factor. More importantly, LDI was >90% accurate at predicting the need for excision and grafting. The accuracy of the decision to debride deep dermal burns to avoid HTS using both clinical parameters and LDI was supported by histological and biochemical measurements.
authors.affiliation: From the Department of Surgery, University of Alberta, Edmonton, Canada.
authors.forename: Tara Lynn
authors.initials: TL
authors.lastname: Stewart
authors.forename: Brandon
authors.initials: B
authors.lastname: Ball
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Schembri
authors.forename: Keijiro
authors.initials: K
authors.lastname: Hori
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.forename: Heather A
authors.initials: HA
authors.lastname: Shankowsky
authors.forename: Edward E
authors.initials: EE
authors.lastname: Tredget
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D002056:Burns / Q000378:metabolism / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D017439:Cicatrix, Hypertrophic / Q000378:metabolism / Q000473:pathology*; D024042:Collagen Type I / Q000378:metabolism; D055513:Connective Tissue Growth Factor / Q000378:metabolism; D005260:Female; D050958:HSP47 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D050958:HSP47 Heat-Shock Proteins; C489183:LSP1 protein, human; D008840:Microfilament Proteins; D016212:Transforming Growth Factor beta; D055513:Connective Tissue Growth Factor