title: Development of the Fc-III tagged protein expression system for protein purification and detection.
abstract: In the present work, we developed the Fc-III tagged protein expression system for protein purification and detection. The Fc-III sequence encodes for a 13 residue peptide and this peptide is cyclized by disulfide bond formation when the fusion protein is expressed. The Fc-III-fusion proteins selectively bind to immunoglobulin Fc domains (IgG-Fc) expressed from E. coli. We showed the efficient purification of Fc-III tagged proteins by immobilized non-native IgG-Fc and the detection of the cellular locations of fusion proteins by fluorescent-conjugated IgG-Fc. Our results prove that Fc-III tagged protein expression system is a simple and efficient tool for protein purification and detection and is a useful addition to the biochemistry and proteomics toolbox.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Feng
authors.forename: Enbing
authors.initials: E
authors.lastname: Tian
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Wang
authors.forename: Haiteng
authors.initials: H
authors.lastname: Deng
mesh_terms: D000595:Amino Acid Sequence; D001671:Biochemistry / Q000379:methods*; D002256:Carbonic Anhydrases / Q000378:metabolism; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D010455:Peptides; D011993:Recombinant Fusion Proteins; D002256:Carbonic Anhydrases