title: Cdx2 contributes to the expansion of the early primordial germ cell population in the mouse.
abstract: Cdx gene products regulate the extent of axial elongation from the posterior growth zone. These transcription factors sustain the emergence of trunk and tail tissues by providing a suitable niche in the axial progenitor zone, via regulation of Wnt signaling. Cdx genes are expressed in and along the complete primitive streak including its posterior part wherefrom the extraembryonic mesoderm of the allantois emerges. Cdx genes are required for the full development of the allantois and its derivatives in the placental labyrinth. The mouse germ cell lineage also originates from the proximo-posterior epiblast of the primitive streak, and is established within the extraembryonic mesoderm that generates the allantois. We asked whether the expression of Cdx genes around the newly specified PGCs is necessary for the maintenance and expansion of this population, as it is for the allantois and axial progenitors. We observed a significantly lower number of PGCs in Cdx2(null) embryos than in controls. We found that Wnt3a loss of function decreases the PGC population to the same extent as Cdx2 inactivation. Moreover, exogenous Wnt3a corrects the lower PGC number in Cdx2(null) posterior embryonic tissues cultured in vitro. Cdx2 is not expressed in PGCs themselves, and we propose that the expression of Cdx2 in posterior extraembryonic tissues contributes to the proper niche of the germ cell progenitors by stimulating canonical Wnt signaling. Since PGC residence within the posterior growth zone is a mouse-specific feature, our data suggest that mouse PGCs opportunistically became dependent on the axial progenitor niche.
authors.affiliation: Hubrecht Institute, Developmental Biology and Stem Cell research and UMC Utrecht, Uppsalalaan 8, 3584CT Utrecht, The Netherlands.
authors.forename: Monika
authors.initials: M
authors.lastname: Bialecka
authors.forename: Teddy
authors.initials: T
authors.lastname: Young
authors.forename: Susana
authors.initials: S
authors.lastname: Chuva de Sousa Lopes
authors.forename: Derk
authors.initials: D
authors.lastname: ten Berge
authors.forename: Anneke
authors.initials: A
authors.lastname: Sanders
authors.forename: Felix
authors.initials: F
authors.lastname: Beck
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Deschamps
mesh_terms: D000482:Allantois / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D000818:Animals; D000071616:CDX2 Transcription Factor; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000166:cytology* / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D000071616:CDX2 Transcription Factor; C487514:Cdx2 protein, mouse; D018398:Homeodomain Proteins; D014157:Transcription Factors