title: Comparative efficiency of microbial enzyme preparations versus pancreatin for in vitro alimentary protein digestion.
abstract: Utilisation of microbial enzymes may represent an alternative strategy to the use of conventional pancreatin obtained from pig pancreas for the treatment of severe pancreatic insufficiency. In this study, we focused on the capacity of two microbial preparations for their capacity to digest alimentary proteins (caseins and soya proteins) in comparison with pancreatin. These microbial enzymatic preparations were found to be able to generate small, medium-size and larger polypeptides from caseins and soya proteins but were inactivated at pH 3.0. As determined by Liquid Chromatography-Mass Spectrometry analysis, microbial enzymes generated very different peptides from caseins when compared with peptides generated through pancreatin action. These microbial preparations were characterised by relatively low trypsin- and low carboxypeptidase-like activities but high chymotrypsin-like activities and strong capacity for cleavage of caseins at the methionine sites. Although the efficiency of these microbial preparations to increase the rate of absorption of nitrogen-containing compounds in severe pancreatic insufficiency remains to be tested in vivo, our in vitro data indicate proteolytic capacities of such preparations for alimentary protein digestion.
authors.affiliation: INRA, CNRH-IdF, AgroParisTech, UMR 914 Nutrition Physiology and Ingestive Behaviour, 16 Rue Claude Bernard, 75005 Paris, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Andriamihaja
authors.forename: Alain
authors.initials: A
authors.lastname: Guillot
authors.forename: Allan
authors.initials: A
authors.lastname: Svendsen
authors.forename: Joerg
authors.initials: J
authors.lastname: Hagedorn
authors.forename: Sahondra
authors.initials: S
authors.lastname: Rakotondratohanina
authors.forename: Daniel
authors.initials: D
authors.lastname: Tomé
authors.forename: François
authors.initials: F
authors.lastname: Blachier
mesh_terms: D000192:Actinomycetales / Q000201:enzymology*; D000818:Animals; D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D002364:Caseins / Q000737:chemistry*; D004063:Digestion; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010194:Pancreatin / Q000737:chemistry*; D010447:Peptide Hydrolases / Q000737:chemistry*; D030262:Soybean Proteins / Q000737:chemistry*; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D002364:Caseins; D030262:Soybean Proteins; D010194:Pancreatin; D010447:Peptide Hydrolases