title: MS-based ligand binding assays with speed, sensitivity, and specificity.
abstract: Immunoassays are widely used in biochemical/clinical laboratories owing to their simplicity, speed, and sensitivity. We combined self-assembled monolayer-based immunoassays with MALDI-TOF MS to show that high-fidelity surface preparations with a novel matrix deposition/crystallization technique permits quantitative analysis of monolayer-bound antigens at picomolar detection limits. Calibration curves for intact proteins are possible over a broad concentration range and improved specificity of MS-immunoassays is highlighted by simultaneous label-free quantitation of ligand-bound protein complexes.
authors.affiliation: Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9185, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Roth
authors.forename: Jaekuk
authors.initials: J
authors.lastname: Kim
authors.forename: Erica M
authors.initials: EM
authors.lastname: Maresh
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Plymire
authors.forename: John R
authors.initials: JR
authors.lastname: Corbett
authors.forename: Junmei
authors.initials: J
authors.lastname: Zhang
authors.forename: Steven M
authors.initials: SM
authors.lastname: Patrie
mesh_terms: D055910:Antibodies, Immobilized / Q000378:metabolism; D000941:Antigens / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D011485:Protein Binding; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D055910:Antibodies, Immobilized; D000941:Antigens; D011506:Proteins