title: Array-based profiling of the differential methylation status of CpG islands in hepatocellular carcinoma cell lines.
abstract: Alterations in the DNA methylation status particularly in CpG islands are involved in the initiation and progression of many types of human cancer. A number of DNA methylation alterations have been reported in hepatocellular carcinoma (HCC). However, a systematic analysis is required to elucidate the relationship between differential DNA methylation status and the characteristics and progression of HCC. In the present study, a global analysis of DNA methylation using a human CpG-island 12K array was performed on a number of HCC cell lines of different origin and metastatic potential. Based on a standard methylation alteration ratio of ≥2 or ≤0.5, 58 CpG island sites and 66 tumor-related genes upstream, downstream or within were identified. This study showed a series of CpG island methylation alterations in the HCC cell lines. The expression of various oncogenes, tumor suppressor genes and other key genes were up- or downregulated, respectively, resulting in CpG island hypomethylation or hypermethylation accordingly. To conclude, a foundation has been provided for screening CpG island methylation profiles as HCC biological markers.
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China.
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Zheng
authors.forename: Yin-Kun
authors.initials: YK
authors.lastname: Liu
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Kang
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
authors.forename: Kun
authors.initials: K
authors.lastname: Guo
authors.forename: Rui-Xia
authors.initials: RX
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao