title: Methylation analysis of idiopathic recurrent spontaneous miscarriage cases reveals aberrant imprinting at H19 ICR in normozoospermic individuals.
abstract: OBJECTIVE
  To study H19 ICR methylation levels in association with sperm parameters routinely analyzed in idiopathic recurrent spontaneous miscarriage cases.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  Academic research setting.
  
  
  METHODS
  Male partners of couples with a history of idiopathic recurrent spontaneous miscarriage (RSM group) and male partners of couples with proven fertility (control group).
  
  
  METHODS
  None.
  
  
  METHODS
  Paternal age, sperm concentration, motility, chromatin compaction status, morphology, and H19 ICR methylation were assessed in control and idiopathic RSM group participants.
  
  
  RESULTS
  Paternal age and basic semen parameters analyzed did not show any significant difference between the two groups; however H19 ICR methylation levels were reduced significantly in the idiopathic RSM group compared with the control group.
  
  
  CONCLUSIONS
  Significant reduction in the H19 ICR methylation without significant difference in the sperm parameters demonstrates aberrant imprinting to be associated with idiopathic RSM.
authors.affiliation: Department of Neuroendocrinology, National Institute for Research in Reproductive Health, Mumbai, India.
authors.forename: Mandar
authors.initials: M
authors.lastname: Ankolkar
authors.forename: Anushree
authors.initials: A
authors.lastname: Patil
authors.forename: Himangi
authors.initials: H
authors.lastname: Warke
authors.forename: Vinita
authors.initials: V
authors.lastname: Salvi
authors.forename: Neelam
authors.initials: N
authors.lastname: Kedia Mokashi
authors.forename: Shilpa
authors.initials: S
authors.lastname: Pathak
authors.forename: N H
authors.initials: NH
authors.lastname: Balasinor
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics*; D000367:Age Factors; D016022:Case-Control Studies; D048430:Cell Shape; D042002:Chromatin Assembly and Disassembly; D019175:DNA Methylation; D015536:Down-Regulation; D005260:Female; D020022:Genetic Predisposition to Disease; D018392:Genomic Imprinting; D006801:Humans; D007194:India; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D008297:Male; D011247:Pregnancy; D062085:RNA, Long Noncoding / Q000235:genetics*; D055101:Semen Analysis / Q000379:methods; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology
chemical_list: C094335:H19 long non-coding RNA; C035170:IGF2 protein, human; D062085:RNA, Long Noncoding; D007335:Insulin-Like Growth Factor II