title: A new in vitro invasion model for oral cancer using an acellular allogenic dermal matrix (Alloderm): the relationship among in vitro invasion activity, in vivo invasion and metastasis.
abstract: The purpose of this study was to develop a new in vitro model of cancer invasion using a human  dermal equivalent, AlloDerm®. A squamous cell carcinoma line (HSC-2, HSC-3 and OSC20) and a malignant  melanoma line (G361) were cultured on AlloDerm® and evaluated histologically to examine destruction of  the basement membrane and invasion into the dermis of AlloDerm®. HSC-3 and G361 cells invaded through  the basement membrane of AlloDerm® at 21-28 days after seeding, while HSC-2 and OSC20 cells did not. Next,  these cells were transplanted into the tongues of nude mice, and the degree of local invasiveness was  examined histologically. HSC-3 and G361 cells invaded diffusely to the surrounding tongue tissue, although  HSC-2 and OSC20 showed only expansive growth. Further, these cells were transplanted subcutaneously in nude  mice to study metastatic activity. Regional and distant metastases were rarely observed after transplantation  of HSC-2 and OSC20 cells. On the other hand, HSC-3 and G361 cells frequently metastasized. These findings  show a close relationship among in vitro invasiveness on AlloDerm®, in vivo invasiveness, and metastatic  activity. This experimental model using AlloDerm® is a potentially new in vitro model of cancer invasion.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Takahashi
authors.forename: Takashi
authors.initials: T
authors.lastname: Shigeta
authors.forename: Masahiro
authors.initials: M
authors.lastname: Umeda
authors.forename: Takahide
authors.initials: T
authors.lastname: Komori
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D003094:Collagen; D005260:Female; D006801:Humans; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008954:Models, Biological; D009062:Mouth Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis
chemical_list: C440568:Alloderm; D003094:Collagen