title: [Construction and identification of small interfering RNA expression vector targeting ATF-2 gene].
abstract: OBJECTIVE
  To construct an eukaryotic expression vector for RNA interference targeting activating transcription factor 2 (ATF-2) gene,  and explore its effect on proliferation and apoptosis of  HepG2 cells.
  
  
  METHODS
  Two complementary oligonucleotides were synthesized based on ATF-2 mRNA sequence. The annealed fragment was inserted into the vector PBA-siU6. The recombinant plasmid PBA-siATF-2 was confirmed by DNA sequencing and transfected into HepG2 cells mediated by liposome. After transfection,  ATF-2 protein was detected by Western blotting. The cellular growth activity and apoptosis rate were measured by MTT assay and flow cytometry,  respectively.
  
  
  RESULTS
  Recombinant plasmid expressing siRNA targeting ATF-2 gene was confirmed by DNA sequencing. Plasmid transfection down-regulated the level of ATF-2 protein in HepG2 cells,   which blocked cellular growth and induced cell apoptosis.
  
  
  CONCLUSIONS
  The eukaryotic expression vector for RNA interference targeting ATF-2 gene was constructed successfully,  which inhibits HepG2 cell  proliferation and induces cell apoptosis.
authors.affiliation: Department of Laboratory Medicine, Faculty of Clinical Medicine, Jiujiang, China.
authors.forename: Wei-wei
authors.initials: WW
authors.lastname: Mao
authors.forename: Peng
authors.initials: P
authors.lastname: Xiong
authors.forename: Feng
authors.initials: F
authors.lastname: Han
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Hu
mesh_terms: D051698:Activating Transcription Factor 2 / Q000235:genetics*; D017209:Apoptosis; D001483:Base Sequence; D049109:Cell Proliferation; D015316:Genetic Therapy; D005822:Genetic Vectors; D056945:Hep G2 Cells; D006801:Humans; D008969:Molecular Sequence Data; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: C497105:ATF2 protein, human; D051698:Activating Transcription Factor 2; D034741:RNA, Small Interfering