title: The prevalence of Acarapis woodi in Spanish honey bee (Apis mellifera) colonies.
abstract: Acarapis woodi is an internal obligate parasite of the respiratory system of honey bees which provokes significant economic losses in many geographical areas. The main aim of this study was assess the A. woodi role in the "higher honey bee colony losses phenomenon" in Spain. Therefore, a new polymerase chain reaction (PCR) was developed to amplify the mitochondrial cytochrome oxidase I gene (COI) and so the actual prevalence of A. woodi in Spanish honey bee colonies in 2006 and 2007 was determined as part of a wider survey. The results revealed a greater prevalence than expected in most of the geographical areas studied where has been generally underestimated One problem encountered in this study was to distinguish between A. woodi and other species (Acarapis dorsalis and Acarapis externus) at the molecular level. Furthermore, the patterns of genetic divergence across sequences raised serious doubts about the current classification of these organisms.
authors.affiliation: Bee Pathology Laboratory, Centro Apícola Regional, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain. megarrido@jccm.es
authors.forename: Encarna
authors.initials: E
authors.lastname: Garrido-Bailón
authors.forename: Carolina
authors.initials: C
authors.lastname: Bartolomé
authors.forename: Lourdes
authors.initials: L
authors.lastname: Prieto
authors.forename: Cristina
authors.initials: C
authors.lastname: Botías
authors.forename: Amparo
authors.initials: A
authors.lastname: Martínez-Salvador
authors.forename: Aránzazu
authors.initials: A
authors.lastname: Meana
authors.forename: Raquel
authors.initials: R
authors.lastname: Martín-Hernández
authors.forename: Mariano
authors.initials: M
authors.lastname: Higes
mesh_terms: D000818:Animals; D001516:Bees / Q000469:parasitology*; D003430:Cross-Sectional Studies; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000235:genetics; D008925:Mites / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D008928:Mitochondria / Q000201:enzymology; D016133:Polymerase Chain Reaction / Q000592:standards; D015995:Prevalence; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D013030:Spain / Q000453:epidemiology
chemical_list: D004247:DNA; D003576:Electron Transport Complex IV