title: Identification of Max binding protein as a novel binding protein of Nck1 and characterization of its role in inhibiting human liver cancer SK-HEP-1 cells.
abstract: BACKGROUND
  The tendency of tumor cells to disperse throughout the liver is a distinct feature of hepatocellular carcinoma (HCC). Nck family adaptor proteins function to regulate actin cytoskeletal reorganization that leads to cell motility. We previously found that Max binding protein (MNT) was differentially expressed in HCC, and interacted with Nck1 by 2-DE. MNT is a protein member of the Myc/Max/Mad network which plays roles in cell proliferation, differentiation, and death. We investigated the effects of MNT on migration of human liver cancer SK-HEP-1 cells to study the migration regulatory role of MNT in HCC cells.
  
  
  METHODS
  Interaction between MNT and Nck1 was further validated in hepatoma cells by GST-pull down assay and immunoprecipitation. siRNAs specific to MNT (MNT siRNA) were used to knockdown MNT expression. Western blotting, transwell assay were used to determine the migration potential of cells.
  
  
  RESULTS
  Interaction between MNT and Nck1 was validated in hepatoma cells. MNT knockdown promoted the migration of human liver cancer SK-HEP-1 cells (P < 0.01).
  
  
  CONCLUSIONS
  The results suggest that MNT, via interaction with Nck1, inhibits hepatoma cell migration.
authors.affiliation: Department of Hepatobiliary Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangdong, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Tao
authors.initials: T
authors.lastname: Huang
authors.forename: Ya-feng
authors.initials: YF
authors.lastname: Wang
authors.forename: Kun-sun
authors.initials: KS
authors.lastname: Zhang
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Bao-gang
authors.initials: BG
authors.lastname: Peng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D006801:Humans; D047468:Immunoprecipitation; D008113:Liver Neoplasms; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000235:genetics / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C106465:MNT protein, human; C078332:Nck protein; D015513:Oncogene Proteins; D012097:Repressor Proteins