title: Enhanced peroxisomal β-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice.
abstract: This study aimed to investigate the potential mechanisms of natural resistance to high-fat diet-induced obesity. Four-week-old C57BL/6 mice were fed a high-fat diet for 6 weeks and were then designated as high-fat diet-fed obesity-prone (HOP) and obesity-resistant (HOR) animals. Their blood biochemistry was evaluated, and visceral adipose tissue samples were subjected to proteomic, Western blot and quantitative real-time PCR (q-PCR) analyses. The HOR mice showed reduced visceral fat weight and size, as well as lowered serum lipid and leptin levels. Proteomic analysis showed that enoyl coenzyme A hydratase 1, peroxisomal (Ech1) expression was significantly increased in their visceral adipose tissues. Moreover, other proteins, such as α-tropomyosin, myosin light chain, urine-nucleoside phosphorylase and transgelin, were also significantly increased. Furthermore, q-PCR analysis showed that the expression of acyl-CoA oxidase 1 palmitoyl, enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase and 3-oxoacyl-CoA thiolase responsible for peroxisomal β-oxidation was also up-regulated in the visceral adipose tissues of the HOR mice. The expression of peroxisome proliferator-activated receptor α (PPARα) was increased in the HOR mice as shown by Western blot analysis. Obesity-resistant animals show enhanced peroxisomal β-oxidation metabolism and reduced fat accumulation in visceral adipose tissues by up-regulating the expression of Ech1, peroxisomal or other related peroxisomal β-oxidation marker genes, which may be driven or enhanced by the up-regulation of the expression of PPARα. However, further validation in future studies is required.
authors.affiliation: Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055;
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Xie
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.forename: Jin-Fang
authors.initials: JF
authors.lastname: Zhang
authors.forename: Jian-Na
authors.initials: JN
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Can
authors.forename: Lv
authors.initials: L
authors.lastname: Qing
authors.forename: Hsiang-Fu
authors.initials: HF
authors.lastname: Kung
authors.forename: Ya-Ou
authors.initials: YO
authors.lastname: Zhang