title: MALDI-based identification of stable hazelnut protein derived tryptic marker peptides.
abstract: Food allergy is an important health problem especially in industrialised countries. Tree nuts, among which are hazelnuts (Corylus avellana), are typically causing serious and life-threatening symptoms in sensitive subjects. Hazelnut is used as a food ingredient in pastry, confectionary products, ice cream and meat products, therefore undeclared hazelnut can be often present as a cross-contaminant representing a threat for allergic consumers. Mass spectrometric techniques are used for the detection of food allergens in processed foods, but limited information regarding stable tryptic peptide markers for hazelnut is available. The aim of this study was to detect stable peptide markers from modified hazelnut protein through the Maillard reaction and oxidation in a buffered solution. Peptides ³⁹⁵Gly-Arg⁴⁰³ from Cor a 11 and ²⁰⁹Gln-Arg²¹⁷, ³⁵¹Ile-Arg³⁶³, ⁴⁶⁴Ala-Arg⁴⁷⁸ and ⁴⁰¹Val-Arg⁴¹⁷ from Cor a 9 hazelnut allergens proved to be the most stable and could be detected and confirmed with high scores in most of the modified samples. The identified peptides can be further used as analytical targets for the development of more robust quantitative methods for hazelnut detection in processed foods.
authors.affiliation: Department of Food Safety and Food Quality, Research Group Food Chemistry and Human Nutrition, Ghent University, Ghent, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: Cucu
authors.forename: B
authors.initials: B
authors.lastname: De Meulenaer
authors.forename: B
authors.initials: B
authors.lastname: Devreese
mesh_terms: D000485:Allergens / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D052179:Antigens, Plant / Q000009:adverse effects / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D031211:Corylus / Q000009:adverse effects / Q000737:chemistry*; D005506:Food Contamination; D005511:Food Handling; D005513:Food Inspection / Q000379:methods*; D006801:Humans; D015416:Maillard Reaction; D021184:Nut Hypersensitivity / Q000517:prevention & control; D009754:Nuts / Q000009:adverse effects / Q000737:chemistry*; D009842:Oligopeptides / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000009:adverse effects / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000009:adverse effects / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D055550:Protein Stability; D055314:Seed Storage Proteins / Q000009:adverse effects / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D015415:Biomarkers; C562011:Cor a 11 allergen, hazelnut; C466060:Cor a 9 protein, hazelnut; D009842:Oligopeptides; D010446:Peptide Fragments; D010940:Plant Proteins; D055314:Seed Storage Proteins