title: Development of a novel europium(III) complex-based luminescence probe for time-resolved luminescence imaging of the nitric oxide production in neuron cells.
abstract: Nitric oxide (NO) is a signaling molecule, and plays an important role in some human neuroses. In this work, a novel europium(III) complex, [4'-(3-methylamino-4-aminophenoxy)-2,2':6',2''-terpyridine-6,6''-diyl] bis(methylenenitrilo) tetrakis(acetate)-Eu(3+) (MATTA-Eu(3+)), has been designed and synthesized as a luminescent probe for the time-resolved luminescence detection of NO. The complex itself is weakly luminescent, but can specifically react with NO under the aerobic conditions to form its triazole derivative, [4'-(3-methyl-4-benzotriazol-6-yl-oxy)-2,2':6',2''-terpyridine-6,6''-diyl] bis(methylenenitrilo) tetrakis(acetate)-Eu(3+) (MBTTA-Eu(3+)), accompanied by a remarkable luminescence enhancement with a long luminescence lifetime. The luminescence response of the complex to NO is rapid, highly selective and sensitive, and pH-insensitive in aqueous solutions. These properties enable MATTA-Eu(3+) to be used as a turn-on luminescent probe for the selective recognition and sensitive time-resolved luminescence detection of NO in a wider pH range. The probe-loaded PC12 cells were prepared, and used for the time-resolved luminescence imaging detection of the glutamate-induced NO production in the cells. The result demonstrated the practical utility of the probe for imaging the NO production in neuron cells.
authors.affiliation: State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116024, PR China.
authors.forename: Mingjing
authors.initials: M
authors.lastname: Liu
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Ye
authors.forename: Guilan
authors.initials: G
authors.lastname: Wang
authors.forename: Jingli
authors.initials: J
authors.lastname: Yuan
mesh_terms: D000818:Animals; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry*; D015195:Drug Design; D005063:Europium / Q000737:chemistry*; D018698:Glutamic Acid / Q000494:pharmacology; D049408:Luminescent Agents / Q000138:chemical synthesis / Q000737:chemistry*; D008163:Luminescent Measurements; D057054:Molecular Imaging / Q000379:methods*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009569:Nitric Oxide / Q000096:biosynthesis*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D016716:PC12 Cells; D051381:Rats; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: C578178:(4'-(3-methylamino-4-aminophenoxy)-2,2':6',2''-terpyridine-6,6''-diyl) bis(methylenenitrilo) tetrakis(acetate)-Eu(III); D056831:Coordination Complexes; D049408:Luminescent Agents; D009942:Organometallic Compounds; D014867:Water; D009569:Nitric Oxide; D018698:Glutamic Acid; D005063:Europium