title: An ATP sensitive light addressable biosensor for extracellular monitoring of single taste receptor cell.
abstract: Adenosine triphosphate (ATP) is considered as the key neurotransmitter in taste buds for taste signal transmission and processing. Measurements of ATP secreted from single taste receptor cell (TRC) with high sensitivity and specificity are essential for investigating mechanisms underlying taste cell-to-cell communications. In this study, we presented an aptamer-based biosensor for the detection of ATP locally secreted from single TRC. ATP sensitive DNA aptamer was used as recognition element and its DNA competitor was served as signal transduction element that was covalently immobilized on the surface of light addressable potentiometric sensor (LAPS). Due to the light addressable capability of LAPS, local ATP secretion from single TRC can be detected by monitoring the working potential shifts of LAPS. The results show this biosensor can detect ATP with high sensitivity and specificity. It is demonstrated this biosensor can effectively detect the local ATP secretion from single TRC responding to tastant mixture. This biosensor could provide a promising new tool for the research of taste cell-to-cell communications as well as for the detection of local ATP secretion from other types of ATP secreting individual cells.
authors.affiliation: Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wu
authors.forename: Liping
authors.initials: L
authors.lastname: Du
authors.forename: Ling
authors.initials: L
authors.lastname: Zou
authors.forename: Luhang
authors.initials: L
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D008027:Light; D008991:Monitoring, Physiologic; D018377:Neurotransmitter Agents / Q000378:metabolism; D011199:Potentiometry / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000502:physiology; D013649:Taste / Q000502:physiology; D013650:Taste Buds / Q000166:cytology* / Q000378:metabolism
chemical_list: D018377:Neurotransmitter Agents; D000255:Adenosine Triphosphate