title: Nitric oxide (NO)-induced death of gram-negative bacteria from a light-controlled NO-releasing platform.
abstract: A NO-delivery platform has been fabricated from polydimethylsiloxane (PDMS) and Pluronic(®) F127 gel that contains the light-sensitive NO donor, [Mn(PaPy(3))(NO)]ClO(4). The material was assembled layer-by-layer. First, a thin PDMS membrane was cast. It was then layered with cold 25% (w/v) Pluronic(®) F127 gel mixed with [Mn(PaPy(3))(NO)]ClO(4). Finally, it was covered with a thick layer (nearly impermeable to NO) of PDMS (=polydimethoxysiloxane) to allow release of NO only from the thinner side upon exposure to light. Light-induced NO release from this layered material has been confirmed via NO-specific electrode and by a modified soft Griess-agar assay. Incorporation of ca. 8 mg/g of [Mn(PaPy(3))(NO)]ClO(4) in the Pluronic gel layer affords a material that drastically reduces the microbial loads of Acinetobacter baumannii and Pseudomonas aeruginosa via the antibiotic effects of the photoreleased NO. Application of this flexible layered NO-donating composite as bandage material has been proposed.
authors.affiliation: Department of Chemistry and Biochemistry, University of California at Santa Cruz, 1156 High Street, Santa Cruz, CA 95062, USA.
authors.forename: Genevieve M
authors.initials: GM
authors.lastname: Halpenny
authors.forename: Brandon
authors.initials: B
authors.lastname: Heilman
authors.forename: Pradip K
authors.initials: PK
authors.lastname: Mascharak
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D016166:Free Radical Scavengers / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D008027:Light; D015394:Molecular Structure; D009569:Nitric Oxide / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D016166:Free Radical Scavengers; D020030:Nitric Oxide Donors; D009757:Nylons; C085707:poly(dimethylsiloxane)-polyamide copolymer; D009569:Nitric Oxide