title: Regulation of human growth and differentiation factor 3 gene expression by NANOG in human embryonic carcinoma NCCIT cells.
abstract: We investigated transactivation by NANOG in regulating growth and differentiation factor 3 (GDF3) expression in NCCIT cells. GDF3 expression was affected by shRNA-mediated downregulation and by exogenous overexpression of NANOG specifically, as well as by retinoic acid-mediated differentiation. GDF3 transcription was activated by NANOG, and the upstream region (-183 to -1) was sufficient to induce minimal transcriptional activity. Moreover, NANOG binds to the GDF3 minimal promoter in vivo and the transcriptional activity is mediated by NANOG transactivation domain. This study provides the first evidence that NANOG is a transcriptional activator of the expression of the oncogenic growth factor GDF3 in embryonic carcinoma cells.
authors.affiliation: Department of Biomedical Science, College of Life Science, CHA University, Gyeonggi-Do, South Korea.
authors.forename: Sung-Won
authors.initials: SW
authors.lastname: Park
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Lim
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Do
authors.forename: BoReum
authors.initials: B
authors.lastname: Sung
authors.forename: Sun-Hyung
authors.initials: SH
authors.lastname: Huh
authors.forename: Sang-Jun
authors.initials: SJ
authors.lastname: Uhm
authors.forename: Hyuk
authors.initials: H
authors.lastname: Song
authors.forename: Hak-Jae
authors.initials: HJ
authors.lastname: Chung
authors.forename: Jin-Hoi
authors.initials: JH
authors.lastname: Kim
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Kim
mesh_terms: D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D018236:Carcinoma, Embryonal / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D055451:Growth Differentiation Factor 3 / Q000235:genetics*; D057809:HEK293 Cells; D018398:Homeodomain Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D000071317:Nanog Homeobox Protein; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D039904:Pluripotent Stem Cells / Q000378:metabolism / Q000473:pathology; D011401:Promoter Regions, Genetic; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology
chemical_list: C525974:GDF3 protein, human; D055451:Growth Differentiation Factor 3; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D034741:RNA, Small Interfering; D015534:Trans-Activators; D014212:Tretinoin