title: Affinity proteomics: the role of specific binding reagents in human proteome analysis.
abstract: Affinity proteomics is the field of proteome analysis based on the use of antibodies and other binding reagents as protein-specific detection probes. In this review, the particular strengths of affinity methods for determination of protein localization, functional characterization, biomarker discovery and intracellular applications, and their resulting impact in basic and clinical research are highlighted. An additional focus is on the requirements for systematic binder generation and current large-scale binder projects, including bioinformatic frameworks for epitope selection and for documentation of available binding reagents and their performance. In addition to current affinity proteomics methods and applications, including arrays of proteins, binders, lysates and tissues, approaches coupling mass spectrometry-based proteomics and affinity proteomics are reviewed.
authors.affiliation: Protein Technology Group, Babraham Bioscience Technologies Ltd, Babraham Research Campus, Cambridge, UK. oda.stoevesandt@babraham.ac.uk
authors.forename: Oda
authors.initials: O
authors.lastname: Stoevesandt
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Taussig
mesh_terms: D000345:Affinity Labels / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015415:Biomarkers / Q000737:chemistry; D019295:Computational Biology / Q000379:methods; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D040081:Protein Array Analysis / Q000379:methods; D025941:Protein Interaction Mapping / Q000379:methods; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012680:Sensitivity and Specificity; D013379:Substrate Specificity
chemical_list: D000345:Affinity Labels; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D000939:Epitopes; D020543:Proteome