title: BDNF expression with functional improvement in transected spinal cord treated with neural stem cells in adult rats.
abstract: Neural stem cells (NSC) could promote the repair after spinal cord transection (SCT), the underlying mechanism, however, still keeps to be defined. This study reported that NSC grafts significantly improved sensory and locomotor functions in adult rats with SCT in acute stage after injury. NSC could survive; differentiate towards neurons or glia lineage in vitro and vivo. Biotin dextran amine (BDA) tracing showed that little CST regeneration in the injury site, while SEP was recorded in NSC engrafted rats. Immunohistochemistry and Real time PCR confirmed that engrafted NSC expressed BDNF and increased the level of BDNF mRNA in injured site following transplantation. The present data therefore suggested that the functional recovery following SCT with NSC transplantation was correlated with the expression of BDNF, indicating the usage of BDNF with NSC transplantation in the treatment of SCI following injury.
authors.affiliation: Institute of Neuroscience, Kunming Medical College, Kunming 650031, China.
authors.forename: Bao-Li
authors.initials: BL
authors.lastname: He
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Ba
authors.forename: Xu-yang
authors.initials: XY
authors.lastname: Wang
authors.forename: Su-juan
authors.initials: SJ
authors.lastname: Liu
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Liu
authors.forename: Shan
authors.initials: S
authors.lastname: Ou
authors.forename: Yin-li
authors.initials: YL
authors.lastname: Gu
authors.forename: Xing-hua
authors.initials: XH
authors.lastname: Pan
authors.forename: Ting-Hua
authors.initials: TH
authors.lastname: Wang
mesh_terms: D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D003911:Dextrans; D005073:Evoked Potentials, Somatosensory / Q000187:drug effects / Q000502:physiology; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006614:Hindlimb / Q000294:innervation / Q000502:physiology; D007150:Immunohistochemistry; D008124:Locomotion / Q000502:physiology; D009043:Motor Activity / Q000187:drug effects; D058953:Neural Stem Cells / Q000637:transplantation*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D020127:Recovery of Function; D013116:Spinal Cord / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000628:therapy*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D003911:Dextrans; D005456:Fluorescent Dyes; D012333:RNA, Messenger; C076397:biotinylated dextran amine; D001710:Biotin