title: Activity of the Arabidopsis RD29A and RD29B promoter elements in soybean under water stress.
abstract: The constitutive and drought-induced activities of the Arabidopsis thaliana RD29A and RD29B promoters were monitored in soybean (Glycine max (L.) Merr.] via fusions with the visual marker gene β-glucuronidase (GUS). Physiological responses of soybean plants were monitored over 9 days of water deprivation under greenhouse conditions. Data were used to select appropriate time points to monitor the activities of the respective promoter elements. Qualitative and quantitative assays for GUS expression were conducted in root and leaf tissues, from plants under well-watered and dry-down conditions. Both RD29A and RD29B promoters were significantly activated in soybean plants subjected to dry-down conditions. However, a low level of constitutive promoter activity was also observed in both root and leaves of plants under well-watered conditions. GUS expression was notably higher in roots than in leaves. These observations suggest that the respective drought-responsive regulatory elements present in the RD29X promoters may be useful in controlling targeted transgenes to mitigate abiotic stress in soybean, provided the transgene under control of these promoters does not invoke agronomic penalties with leaky expression when no abiotic stress is imposed.
authors.affiliation: School of Natural Resources, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.
authors.forename: Saadia
authors.initials: S
authors.lastname: Bihmidine
authors.forename: Jiusheng
authors.initials: J
authors.lastname: Lin
authors.forename: Julie M
authors.initials: JM
authors.lastname: Stone
authors.forename: Tala
authors.initials: T
authors.lastname: Awada
authors.forename: James E
authors.initials: JE
authors.lastname: Specht
authors.forename: Tom E
authors.initials: TE
authors.lastname: Clemente
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics*; D015139:Blotting, Southern; D055864:Droughts; D005470:Fluorometry; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D014867:Water / Q000494:pharmacology*
chemical_list: D029681:Arabidopsis Proteins; C520629:RD29a protein, Arabidopsis; D014867:Water; D005966:Glucuronidase