title: Acetylcholinesterase immobilized on magnetic beads for pesticides detection: application to olive oil analysis.
abstract: This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on the covalent immobilisation of acetylcholinesterase on magnetic microbeads using either colorimetry or amperometry as detection technique. The magnetic beads were immobilised on screen-printed electrodes or microtitration plates and tested using standard solutions and real samples. The developed devices showed good analytical performances with limits of detection much lower than the maximum residue limit tolerated by international regulations, as well as a good reproducibility and stability.
authors.affiliation: IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Perpignan Cedex, France. najwa_benoujji@hotmail.com
authors.forename: Najwa
authors.initials: N
authors.lastname: Ben Oujji
authors.forename: Idriss
authors.initials: I
authors.lastname: Bakas
authors.forename: Georges
authors.initials: G
authors.lastname: Istamboulié
authors.forename: Ihya
authors.initials: I
authors.lastname: Ait-Ichou
authors.forename: Elhabib
authors.initials: E
authors.lastname: Ait-Addi
authors.forename: Régis
authors.initials: R
authors.lastname: Rouillon
authors.forename: Thierry
authors.initials: T
authors.lastname: Noguer
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D015374:Biosensing Techniques; D003124:Colorimetry; D004117:Dimethoate / Q000031:analogs & derivatives / Q000032:analysis; D055664:Electrochemical Techniques; D004800:Enzymes, Immobilized / Q000378:metabolism*; D020128:Inhibitory Concentration 50; D007306:Insecticides / Q000032:analysis / Q000737:chemistry; D007700:Kinetics; D008280:Magnetics; D008294:Malathion / Q000031:analogs & derivatives / Q000032:analysis; D008863:Microspheres; D000069463:Olive Oil; D010755:Organophosphates / Q000032:analysis / Q000737:chemistry; D009946:Organothiophosphorus Compounds / Q000032:analysis; D010575:Pesticides / Q000032:analysis* / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D013997:Time Factors
chemical_list: D004800:Enzymes, Immobilized; D007306:Insecticides; D000069463:Olive Oil; D010755:Organophosphates; D009946:Organothiophosphorus Compounds; D010575:Pesticides; D010938:Plant Oils; C100082:malaoxon; C002302:dimethoxon; D000110:Acetylcholinesterase; D008294:Malathion; D004117:Dimethoate; C005828:methidathion