title: Renal allograft recipients fail to increase interferon-γ during invasive fungal diseases.
abstract: Invasive fungal diseases are a major cause of death in renal allograft recipients. We previously reported that adjunctive recombinant human interferon-γ therapy has clinical utility for invasive fungal diseases after renal transplantation. We have now developed a rapid peripheral blood-based quantitative real-time PCR assay that enables accurate profiling of cytokine imbalances. Our preliminary studies in renal transplant patients with invasive fungal diseases suggest that they fail to mount an adequate interferon-γ response to the fungal infection. In addition, they have reduced IL-10 and increased TNF-α when compared to stable renal transplant patients. These preliminary cytokine profiling-based observations provide a possible explanation for the therapeutic benefit of adjunctive human interferon-γ therapy in renal allograft recipients with invasive fungal diseases.
authors.affiliation: Section of Infectious Diseases and Immunity, Department of Medicine, Imperial College London, London, UK.
authors.forename: D
authors.initials: D
authors.lastname: Armstrong-James
authors.forename: I
authors.initials: I
authors.lastname: Teo
authors.forename: S
authors.initials: S
authors.lastname: Herbst
authors.forename: M
authors.initials: M
authors.lastname: Petrou
authors.forename: K Y
authors.initials: KY
authors.lastname: Shiu
authors.forename: A
authors.initials: A
authors.lastname: McLean
authors.forename: D
authors.initials: D
authors.lastname: Taube
authors.forename: A
authors.initials: A
authors.lastname: Dorling
authors.forename: S
authors.initials: S
authors.lastname: Shaunak
mesh_terms: D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D003587:Cytomegalovirus / Q000235:genetics; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D004247:DNA / Q000097:blood / Q000235:genetics; D005500:Follow-Up Studies; D006084:Graft Rejection / Q000097:blood / Q000175:diagnosis* / Q000209:etiology; D006801:Humans; D007371:Interferon-gamma / Q000097:blood* / Q000235:genetics; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics; D016030:Kidney Transplantation / Q000009:adverse effects*; D060888:Real-Time Polymerase Chain Reaction; D014184:Transplantation, Homologous; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000235:genetics
chemical_list: D015415:Biomarkers; C573415:IFNG protein, human; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma; D004247:DNA