title: Effect of amino acids on expression of signal molecules in organotypic culture of the spleen.
abstract: Opposite effects of amino acids on proliferation and differentiation of immune cells in organotypic culture of the spleen were demonstrated. It was found that methionine stimulates differentiation of CD5(+) lymphocyte precursors into T-helpers, cytotoxic T lymphocytes, and B cells and induces proliferation of CD68(+)-macrophages. Glutamic acid and arginine induce proliferation of B cells, while histidine and leucine promote differentiation of precursors towards cytotoxic T cells. The opposite effects of amino acids on immune cells of the spleen are related to different hydrophobicity of their side chains determining the type of intermolecular interaction.
authors.affiliation: St. Petersburg Institute of Bioregulation and Gerontology, Northwestern Division of the Russian Academy of Medical Sciences, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Kontcevaya
authors.forename: N S
authors.initials: NS
authors.lastname: Linkova
authors.forename: N I
authors.initials: NI
authors.lastname: Chalisova
authors.forename: A V
authors.initials: AV
authors.lastname: Dudkov
authors.forename: D A
authors.initials: DA
authors.lastname: Sinyachkin
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D015704:CD4 Antigens / Q000378:metabolism; D018956:CD5 Antigens / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D000097382:CD68 Molecule
chemical_list: D000596:Amino Acids; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D015704:CD4 Antigens; D018956:CD5 Antigens; C067980:CD68 antigen, human; D000097382:CD68 Molecule