title: Assessment of intramural blood flow and neurogenic control in intact and hypertrophic urinary bladder with harmonic analysis of bioimpedance in rats.
abstract: High-resolution impedancometry and harmonic (Fourier) analysis of variable component of bioimpedance revealed rhythmic oscillations of urinary bladder bioimpedance at the Mayer wave, respiration, and heartbeat frequencies. The power values of the corresponding Mayer, respiratory, and cardiac peaks were calculated to assess circulation in the urinary bladder wall and its autonomic nervous control at various stages of infusion cystometry in intact rats and in the rats with preliminary formed infravesical obstruction (IVO). In intact rats, filling of the bladder with physiological saline diminished the power of the first (fundamental) cardiac peak attesting to a decrease of the blood flow in the bladder wall. Simultaneously, the power of low-frequency Mayer peak reflecting sympathetic activity increased, while the power of respiratory peak decreased supposedly reflecting abatement of the parasympathetic influences. Bladder voiding was accompanied by a decrease of Mayer peak and increase of the respiratory one. Prior to infusion cystometry, the intravesical pressure in IVO rats was elevated while the power of fundamental cardiac peak was below the control value. Filling the bladder in these rats was accompanied by further decrease of the cardiac peak reflecting still greater drop in blood supply. In control rats, voiding the bladder normalized the vesical circulation assessed by the cardiac peak, while in IVO rats this peak remained decreased. The reciprocal changes of Mayer and respiratory peaks observed during infusion cystometry in the norm were replaced by unidirectional decrease in the power of both peaks in IVO rats, which probably attest to disturbance of autonomic nervous control in the hypertrophic urinary bladder in these rats.
authors.affiliation: Department of Experimental Modeling of Urological Diseases, FSA Research Institute of Urology, the Russian Ministry of Health and Social Welfare, Moscow, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Kirpatovsky
authors.forename: I S
authors.initials: IS
authors.lastname: Mudraya
authors.forename: S V
authors.initials: SV
authors.lastname: Revenko
authors.forename: A V
authors.initials: AV
authors.lastname: Nesterov
authors.forename: I Yu
authors.initials: IY
authors.lastname: Gavrilov
authors.forename: R A
authors.initials: RA
authors.lastname: Khromov
authors.forename: A Yu
authors.initials: AY
authors.lastname: Bablumyan
mesh_terms: D000818:Animals; D017776:Autonomic Pathways / Q000502:physiology*; D017097:Electric Impedance; D005260:Female; D006339:Heart Rate / Q000502:physiology; D006984:Hypertrophy / Q000473:pathology*; D011312:Pressure; D051381:Rats; D012039:Regional Blood Flow / Q000502:physiology*; D056152:Respiratory Rate / Q000502:physiology; D001743:Urinary Bladder / Q000098:blood supply* / Q000294:innervation* / Q000473:pathology