title: Protective effects of the key compounds isolated from Corni fructus against β-amyloid-induced neurotoxicity in PC12 cells.
abstract: β-Amyloid (Aβ) peptide is the major component of senile plaques and is considered to have a causal role in the development and progression of Alzheimer’s disease (AD). There is compelling evidence supporting the notion that Aβ-induced cytotoxicity is mediated though the generation of ROS. In the present study, we investigated the neuroprotective effects of ursolic acid (UA), p-coumaric acid (p-CA), and gallic acid (GA) isolated from Corni fructus (CF) against Aβ(25-35)-induced toxicity in PC12 cell. Exposure of PC12 cells to 50 μM Aβ(25-35) increased cellular oxidative stress, the number of apoptotic cells and caspase-3 activity and finally caused significant cell death. However, UA, p-CA, and GA not only suppressed the generation of ROS but also attenuated DNA fragmentation and eventually attenuated Aβ-induced apoptosis in a dose-dependent manner. In protecting cells against Aβ neurotoxicity, UA and GA possessed stronger ability against ROS generation than p-CA, while p-CA showed the strongest anti-apoptotic activity. Particularly, p-CA protected cells at the concentration range from 0.5 up to 125 μM without any adverse effect. Taken together, these effects of UA, p-CA, and GA may be partly associated with the neuroprotective effect of CF. Furthermore, our findings might raise a possibility of therapeutic applications of CF for preventing and/or treating neurodegenerative diseases.
authors.affiliation: Department of Food Science and Nutrition, Dong-A University, Busan 604-714, Korea.
authors.forename: Seung-Young
authors.initials: SY
authors.lastname: Hong
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Jeong
authors.forename: Mira
authors.initials: M
authors.lastname: Jun
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D029768:Cornus / Q000737:chemistry*; D003373:Coumaric Acids / Q000302:isolation & purification / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D005707:Gallic Acid / Q000302:isolation & purification / Q000494:pharmacology*; D019636:Neurodegenerative Diseases / Q000188:drug therapy; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D016716:PC12 Cells; D010446:Peptide Fragments / Q000633:toxicity*; D058225:Plaque, Amyloid / Q000188:drug therapy; D011422:Propionates; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D000097245:Ursolic Acid
chemical_list: D016229:Amyloid beta-Peptides; D000975:Antioxidants; D053148:Caspase 3; D003373:Coumaric Acids; D005707:Gallic Acid; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D011422:Propionates; D017382:Reactive Oxygen Species; D014315:Triterpenes; D000097245:Ursolic Acid; C074636:amyloid beta-protein (25-35); C495469:p-coumaric acid