title: Anti-inflammatory effects of phlorofucofuroeckol B-rich ethyl acetate fraction obtained from Myagropsis myagroides on lipopolysaccharide-stimulated RAW 264.7 cells and mouse edema.
abstract: Myagropsis myagroides has been used as a Chinese medicine and its extract has shown various biological activities, however, its anti-inflammatory mechanism remains unknown. In this study, we investigated the inhibitory effects of the ethyl acetate fraction of M. myagroides (EFM) on the production of inflammatory mediators and pro-inflammatory cytokines in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells. EFM significantly inhibited LPS-induced production of nitric oxide (NO), prostaglandin E(2), and pro-inflammatory cytokines in a dose-dependent manner and suppressed the production of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 in RAW 264.7 cells. Inhibitory effect of EFM on iNOS expression and NO production was further confirmed using LPS-activated mouse peritoneal macrophages. EFM treatment strongly suppressed the activation of nuclear factor-kappa B (NF-κB) by suppressing phosphorylation of Akt and extracellular signal-regulated kinases (ERKs). EFM as well as phlorofucofuroeckol B (PFF-B), a major compound isolated from EFM, reduced ear edema induced by phorbol 12-myristate 13-acetate in mice. These results indicate that the anti-inflammatory effect of EFM, rich in PFF-B, on LPS-stimulated macrophages is regulated by the inhibition of NF-κB pathway through the inhibition of ERKs and Akt phosphorylation in LPS-stimulated macrophage cells.
authors.affiliation: Department of Food Science and Nutrition, Pukyong National University, Busan 608-737, South Korea.
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Joung
authors.forename: Min-Sup
authors.initials: MS
authors.lastname: Lee
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Choi
authors.forename: Jong-Soon
authors.initials: JS
authors.lastname: Kim
authors.forename: Taisun
authors.initials: T
authors.lastname: Shin
authors.forename: Bok-Mi
authors.initials: BM
authors.lastname: Jung
authors.forename: Jae-Il
authors.initials: JI
authors.lastname: Kim
authors.forename: Hyeung-Rak
authors.initials: HR
authors.lastname: Kim
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004147:Dioxins / Q000737:chemistry* / Q000494:pharmacology*; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D000459:Phaeophyceae / Q000737:chemistry*
chemical_list: D001572:Benzofurans; D004147:Dioxins; D008070:Lipopolysaccharides; C516536:phlorofucofuroeckol B