title: Nitric oxide fumigation stimulates flavonoid and phenolic accumulation and enhances antioxidant activity of mushroom.
abstract: The effects of nitric oxide (NO) on antioxidant activity and contents of phenolics and flavonoids in mushroom Russula griseocarnosa were investigated. Freshly harvested mushrooms were fumigated with 0, 10, 20 and 30μLL(-1) NO at 20°C for 2h and then taken to examine the antioxidant activities using assays of reducing power, chelating effect on ferrous ions, scavenging effect on hydroxyl free radicals, and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. The results showed that the antioxidant activities of the mushrooms fumigated with NO were significantly increased when compared to the controls. Moreover, NO fumigation significantly enhanced phenolic and flavonoid contents and stimulated the activities of phenylalanine ammonia-lyase and chalcone synthase. The results indicated that NO fumigation might have potential application for enhancing the bioactive compounds and improving antioxidant activities in the mushrooms. Furthermore, the data suggested that the NO-induced phenolic and flavonoid accumulation was due to the activation of the biosynthetic pathways in the mushrooms.
authors.affiliation: Key Lab of Medical Plant Genetic Improvement and Quality Control of Zhejiang Province, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310065, China.
authors.forename: Jufang
authors.initials: J
authors.lastname: Dong
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Lu
authors.forename: Lina
authors.initials: L
authors.lastname: Sun
authors.forename: Maojun
authors.initials: M
authors.lastname: Xu
mesh_terms: D000363:Agaricales / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000378:metabolism*; D005651:Fumigation; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D009569:Nitric Oxide / Q000737:chemistry / Q000494:pharmacology*; D010636:Phenols / Q000032:analysis / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D005656:Fungal Proteins; D010636:Phenols; D009569:Nitric Oxide