title: Expression of a novel sphingosine 1-phosphate receptor motif protein gene in maturing rat testes.
abstract: We screened a novel sphingosine 1-phosphate receptor type 5 motif-containing gene, LOC290876, from maturing rat testes by differential display. Gene expression was testis-specific, increased at week 7, and continued for 15 weeks. PCR analysis clarified two gene transcript isoforms, which were expressed at the same level in all samples detected in Northern blot. The deduced amino acid sequences of the two isoforms revealed differences in carboxyl terminal sequences. Gene and protein expression in the testes was dominant in the spermatocytes, and protein expression was localized to the nucleus. Taken together, these findings suggest that the LOC290876-encoded gene product is not involved in sphingosine signaling, but has distinct roles in the nucleus during the processes of spermatocyte maturation and meiosis producing spermatids.
authors.affiliation: Laboratory of Veterinary Biochemistry, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Ahmed Magzoub
authors.initials: AM
authors.lastname: Khalid
authors.forename: Atsushi
authors.initials: A
authors.lastname: Asano
authors.forename: Yoshinao Z
authors.initials: YZ
authors.lastname: Hosaka
authors.forename: Kenji
authors.initials: K
authors.lastname: Ohyama
authors.forename: Masanori
authors.initials: M
authors.lastname: Ohta
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Yamano
mesh_terms: D017398:Alternative Splicing; D020816:Amino Acid Motifs; D000818:Animals; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008540:Meiosis / Q000235:genetics; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D049349:Receptors, Lysosphingolipid / Q000235:genetics* / Q000378:metabolism; D013087:Spermatids / Q000166:cytology / Q000378:metabolism*; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D049349:Receptors, Lysosphingolipid