title: Antibacterial activity of bark of Alnus pendula against methicillin-resistant Staphylococcus aureus.
abstract: OBJECTIVE
  Methicillin-resistant Staphylococcus aureus (MRSA) infections are a rapidly growing health problem around the globe. Recently, there has been considerable interest in the use of plant materials as an alternative method to control pathogenic microorganisms. In this study we evaluated the antibacterial activity of bark of Alnus pendula against MRSA.
  
  
  METHODS
  The MIC determination was done using the microdilution broth method and bacterial growth was determined by measuring optical density using spectrophotometer.
  
  
  RESULTS
  Alnus pendula bark EtOH extract and fractions (F-1, -2, -3 and -4) were investigated against MRSA. The most active fractions (F-3 and F-4) led to the isolation of oregonin (ORE) and hirsutanone (HIR). These compounds were active against MRSA strains with minimum inhibitory concentrations (MICs) ranging from 31.25 to 250 microg/ml MIC and 2 MIC of HIR completely inhibited the growth of MRSA.
  
  
  CONCLUSIONS
  The bark EtOH extract of Alnus Pendula has potent antibacterial activity against MRSA.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang Oriental Medicines Research Institute, Jeonbuk, Republic of South Korea.
authors.forename: J G
authors.initials: JG
authors.lastname: Choi
authors.forename: M W
authors.initials: MW
authors.lastname: Lee
authors.forename: S E
authors.initials: SE
authors.lastname: Choi
authors.forename: M H
authors.initials: MH
authors.lastname: Kim
authors.forename: O H
authors.initials: OH
authors.lastname: Kang
authors.forename: Y S
authors.initials: YS
authors.lastname: Lee
authors.forename: H S
authors.initials: HS
authors.lastname: Chae
authors.forename: B
authors.initials: B
authors.lastname: Obiang-Obounou
authors.forename: Y C
authors.initials: YC
authors.lastname: Oh
authors.forename: M R
authors.initials: MR
authors.lastname: Kim
authors.forename: D W
authors.initials: DW
authors.lastname: Shin
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: D Y
authors.initials: DY
authors.lastname: Kwon
mesh_terms: D029661:Alnus / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D036381:Diarylheptanoids / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry; D016106:Methicillin Resistance; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D024301:Plant Bark; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry; D013053:Spectrophotometry
chemical_list: D000900:Anti-Bacterial Agents; D036381:Diarylheptanoids; D010936:Plant Extracts; D012997:Solvents; C409456:oregonin; D000431:Ethanol; C442173:hirsutanone