title: Role of circulating MSCs in vocal fold wound healing.
abstract: OBJECTIVE
  Vocal fold injury can cause intractable scarring resulting in dysphonia. Mesenchymal stem cells (MSCs) have great therapeutic potential in wound healing. They continuously circulate in the peripheral blood and migrate into wound sites where they induce regenerative effects. However, their roles in vocal fold wound healing are poorly understood because few MSCs exist in the peripheral blood and there is no specific marker to identify them. The present study evaluates how intravenously injected MSCs affect vocal fold wound healing using Green Fluorescent Protein (GFP) -labeled MSCs.
  
  
  METHODS
  Prospective study using animal model.
  
  
  METHODS
  GFP-labeled MSCs were obtained from femurs of GFP transgenic Sprague-Dawley rats and incubated in culture. Sprague-Dawley rats underwent intravenous injection of GFP-labeled MSCs (1.0 × 10(6) cells) immediately after vocal fold injury. Histological examination was performed.
  
  
  RESULTS
  Injected MSCs were distributed throughout the vocal fold wound site from day 1 up to day 56. These vocal folds showed increased hepatocyte growth factor (HGF)-positive cells within the wound and improved wound healing compared with sham-treated folds.
  
  
  CONCLUSIONS
  Circulating MSCs can migrate to vocal fold wound sites and upregulate the expression of HGF during wound healing; thus, they are considered to play a significant role in wound healing within the vocal folds.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ohno
authors.forename: Shigeru
authors.initials: S
authors.lastname: Hirano
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Kanemaru
authors.forename: Masanobu
authors.initials: M
authors.lastname: Mizuta
authors.forename: Seiji
authors.initials: S
authors.lastname: Ishikawa
authors.forename: Ichiro
authors.initials: I
authors.lastname: Tateya
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Nakamura
authors.forename: Juichi
authors.initials: J
authors.lastname: Ito
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000502:physiology; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D011446:Prospective Studies; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric; D014827:Vocal Cords / Q000293:injuries*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D017228:Hepatocyte Growth Factor