title: Identification of human remains by DNA analysis of the gastrointestinal contents of fly larvae.
abstract: Dipterous fly larvae (maggots) are frequently collected from a corpse during a criminal investigation. Previous studies showed that DNA analysis of the gastrointestinal contents of maggots might be used to reveal the identity of a victim. However, this approach has not been used to date in legal investigations, and thus its practical usefulness is unknown. A badly burned body was discovered with its face and neck colonized by fly larvae. Given the condition of the body, identification was not possible. Short tandem repeat (STR) typing was performed using the gastrointestinal contents of maggots collected from the victim and was compared to STR profiles obtained from the alleged father. The probability of paternity was 99.685%. Thus, this comparative DNA test enabled the conclusive identification of the remains. This is the first reported case of analysis of human DNA isolated from the gastrointestinal tract of maggots used to identify a victim in a criminal case.
authors.affiliation: Departamento de Patología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Ave. Madero y Dr. Aguirre Pequeño, Colonia Mitras Centro, Monterrey, Nuevo León, México.
authors.forename: María
authors.initials: M
authors.lastname: de Lourdes Chávez-Briones
authors.forename: Raquel
authors.initials: R
authors.lastname: Hernández-Cortés
authors.forename: Porfirio
authors.initials: P
authors.lastname: Díaz-Torres
authors.forename: Alberto
authors.initials: A
authors.lastname: Niderhauser-García
authors.forename: Jesús
authors.initials: J
authors.lastname: Ancer-Rodríguez
authors.forename: Gilberto
authors.initials: G
authors.lastname: Jaramillo-Rangel
authors.forename: Marta
authors.initials: M
authors.lastname: Ortega-Martínez
mesh_terms: D053523:Amelogenin / Q000235:genetics; D000818:Animals; D002056:Burns / Q000473:pathology; D016172:DNA Fingerprinting / Q000379:methods*; D004175:Diptera; D005247:Feeding Behavior; D005260:Female; D005766:Gastrointestinal Contents / Q000737:chemistry*; D056426:Genetic Loci; D006801:Humans; D007814:Larva; D018895:Microsatellite Repeats; D010334:Paternity; D011180:Postmortem Changes
chemical_list: D053523:Amelogenin