title: A three-channel fluorescent probe that distinguishes peroxynitrite from hypochlorite.
abstract: A novel fluorescent probe for peroxynitrite, PN(600), was rationally designed on the basis of a unique fluorophore assembly approach. PN(600) is a green-emitting coumarin derivative. Upon oxidation by peroxynitrite, PN(600) is transformed into a highly fluorescent red-emitting resorufin derivative via an orange-emitting intermediate. This three-channel signaling capability enables PN(600) to differentiate peroxynitrite from other reactive oxygen and nitrogen species, including hypochlorite and hydroxyl radical. Moreover, PN(600) is membrane-permeable and compatible with common TRITC filter sets and displays low cytotoxicity. Therefore, PN(600) is a promising candidate for in vitro peroxynitrite imaging.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
authors.forename: Quanjuan
authors.initials: Q
authors.lastname: Zhang
authors.forename: Zhichuan
authors.initials: Z
authors.lastname: Zhu
authors.forename: Yongli
authors.initials: Y
authors.lastname: Zheng
authors.forename: Jiagao
authors.initials: J
authors.lastname: Cheng
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Long
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.forename: Xuhong
authors.initials: X
authors.lastname: Qian
authors.forename: Youjun
authors.initials: Y
authors.lastname: Yang
mesh_terms: D002463:Cell Membrane Permeability / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D005910:Glioma / Q000188:drug therapy*; D006801:Humans; D006997:Hypochlorous Acid / Q000737:chemistry*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D030421:Peroxynitrous Acid / Q000737:chemistry*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes; D030421:Peroxynitrous Acid; D006997:Hypochlorous Acid