title: Amperometric flow system for blood glucose determination using an immobilized enzyme magnetic reactor.
abstract: An amperometric flow system for glucose determination in blood serum samples after enzymatic reaction with glucose oxidase immobilized on magnetite covered with silica gel modified propylamine is described. The solid was magnetically retained on a mini-column and placed into the flow injection system preceding the amperometric detector using a modified screen printed electrode with [Fe(tris(3,5-dimetyl-1-pyrazolyl)borate)(2)](+)[FeCl(4)](-). The variables involved in the system such as flow rate, enzyme concentration, injection volume and reaction coil length were evaluated using a Taguchi parameter design. Under optimal conditions, the calibration curve of glucose sample was linear between 0.24 and 6.00 mM, and with a limit of detection of 0.08 mM. The repeatability for a 4.0mM glucose solution was 1.0%.The method was validated by comparing the obtained results to those provided by the enzymatic spectrophotometric method; no significant differences were observed.
authors.affiliation: Centro de Investigaciones Quimicas, Universidad Autonoma el Estado de Hidalgo, Carr. Pachuca-Tulancingo km 4.5, C.P. 42076, Pachuca, Hidalgo, Mexico.
authors.forename: Prisciliano
authors.initials: P
authors.lastname: Hernandez
authors.forename: Jose A
authors.initials: JA
authors.lastname: Rodriguez
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Galan
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Castrillejo
authors.forename: Enrique
authors.initials: E
authors.lastname: Barrado
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis* / Q000737:chemistry; D003217:Conductometry / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase