title: Dietary exposure to polychlorinated dibenzo-p-dioxins and dibenzofurans via fish consumption and dioxin-like activity in fish determined by H4IIE-luc bioassay.
abstract: Dietary exposure to polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) via fish consumption in two major electronic (e) waste sites: Guiyu (GY), Guangdong Province and Taizhou (TZ), Zhejiang Province, and dioxin-like activity in fish determined by H4IIE-luc bioassay. In the present study, all fish were below EU's maximum allowable concentration in muscle of fish (4 pg WHO-TEQ/g wet wt), except crucian (4.28 pg WHO-TEQ/g wet wt) and silver carps (7.49 pg WHO-TEQ/g wet wt) collected from GY rivers. Moreover, the residual concentration in bighead carp collected from GY (2.15 pg WHO-TEQ/g wet wt) was close to the EU's action level (3 pg WHO-TEQ/g wet wt) which gives "early warning" to the competent authorities and operators to take measures to eliminate contamination. In addition, results indicated that the maximum human intake of PCDD/Fs via freshwater fish consumption in GY was 4.31 pg WHO-TEQ/kg bw/day, which exceeds the higher end of the tolerable daily intake recommended by the WHO, EC-SCF and JECFA (1-4, 2 and 2.3 pg WHO-TEQ/kg bw/day respectively). Furthermore, H4IIE-luc cell bioassay provides a very sensitive and cost-efficient screening tool for assessing the overall dioxin-like toxicity in the study, and is therefore valuable for high-throughput environmental monitoring studies.
authors.affiliation: Croucher Institute for Environmental Sciences, and Department of Biology, Hong Kong Baptist University, Hong Kong SAR, PR China; School of Biological Sciences, The University of Hong Kong, Kadoorie Biological Sciences Building, Pokfulam, Hong Kong, PR China.
authors.forename: Janet Kit Yan
authors.initials: JK
authors.lastname: Chan
authors.forename: Yu Bon
authors.initials: YB
authors.lastname: Man
authors.forename: Guan Hua
authors.initials: GH
authors.lastname: Xing
authors.forename: Sheng Chun
authors.initials: SC
authors.lastname: Wu
authors.forename: Margaret B
authors.initials: MB
authors.lastname: Murphy
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Ming H
authors.initials: MH
authors.lastname: Wong
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000009:adverse effects* / Q000032:analysis; D001681:Biological Assay / Q000379:methods; D002347:Carps / Q000378:metabolism; D002681:China; D000072338:Dibenzofurans, Polychlorinated; D004032:Diet / Q000009:adverse effects*; D004147:Dioxins / Q000032:analysis*; D004781:Environmental Exposure / Q000009:adverse effects / Q000032:analysis; D005399:Fishes / Q000378:metabolism*; D006801:Humans; D008452:Maximum Allowable Concentration; D018482:Muscle, Skeletal / Q000737:chemistry; D000072317:Polychlorinated Dibenzodioxins / Q000009:adverse effects / Q000031:analogs & derivatives* / Q000032:analysis; D017210:Tilapia / Q000378:metabolism
chemical_list: D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; C023614:dibenzofuran