title: [Effects of Bushen Zhuangjin Decoction containing serum on the apoptosis of chondrocytes induced by mechanics stimulus].
abstract: OBJECTIVE
  To study the effects of Bushen Zhuangjin Decoction (BZD) containing serum on the apoptosis of chondrocytes induced by mechanics stimulus.
  
  
  METHODS
  The BZD containing serum was extracted. The chondrocyte nutritive media was divided into 3 groups, i.e., the common nutritive medium group, the blank rabbit serum medium group, and the BZD nutritive medium group. The apoptosis of chondrocytes was induced by continuing mechanics stimulus in 24 h. Then the chondrocytes were collected. The apoptosis rate of chondrocytes was determined by flow cytometry. The contents of interleukin 1beta (IL-1beta) and nitric oxide (NO) in the corresponding media were determined.
  
  
  RESULTS
  The apoptosis of chondrocytes in the BZD nutritive medium group (19.55 +/- 7.98)% was lower than that of the common nutritive medium group (39.32 +/- 13.45)% and the blank rabbit serum medium group (37.87 +/- 9.67)%, showing statistical difference (P < 0.05). The contents of IL-1beta and NO were also lower in the BZD nutritive medium group with statistical difference when compared with those of the other two groups (P < 0.05).
  
  
  CONCLUSIONS
  BZD containing serum could protect mechanics stimulus induced apoptosis of chondrocytes.
authors.affiliation: Department of Orthopedics, Huzhou Central Hospital of Zhejiang Province, Zhejiang.
authors.forename: Guo-Shun
authors.initials: GS
authors.lastname: Zhou
authors.forename: Xiong-Feng
authors.initials: XF
authors.lastname: Li
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Guan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002358:Cartilage, Articular / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005434:Flow Cytometry; D053583:Interleukin-1beta / Q000032:analysis; D009569:Nitric Oxide / Q000032:analysis; D011817:Rabbits; D044967:Serum
chemical_list: D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; C000610522:bushen zhuangjin; D009569:Nitric Oxide