title: Downregulation of MSP58 suppresses cell proliferation in neuroblastoma cell lines.
abstract: MSP58, a novel oncogene, shows transforming activity in mouse embryonic fibroblasts. However, the oncogenic role of MSP58 in tumor cells has not been fully characterized. To extend understanding of how this protein operates in tumorigenesis, we aimed to identify the effect of MSP58 on neuroblastoma cell proliferation. Here, we found that MSP58 was highly expressed in neuroblastoma tumor samples and cell lines. We found that the majority of MSP58 protein can be detected in the nucleus as reported in other cells. Moreover, MSP58-targeted shRNA lentivirus attenuated neuroblastoma cell proliferation. Knockdown of MSP58 resulted in S-phase cell accumulation, which was accompanied by changes in cell cycle-related molecules. These results indicate that MSP58 plays an oncogenic role in the proliferation of neuroblastoma cells and could be a novel target for the treatment of neuroblastoma.
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular Biology, Xi'an, Shaanxi, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Zhang
authors.forename: Huai-zhou
authors.initials: HZ
authors.lastname: Qin
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.forename: Jiang
authors.initials: J
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics*; D055785:Gene Knockdown Techniques / Q000379:methods; D006801:Humans; D058990:Molecular Targeted Therapy; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics*
chemical_list: C113292:MCRS1 protein, human; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins