title: ZBRK1 represses HIV-1 LTR-mediated transcription.
abstract: The identification of cellular proteins that interact with the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) provides a basic understanding of HIV-1 gene expression, which is the major determinant regulating virus replication. We show that ZBRK1 negatively regulates the HIV-1 LTR. Ectopic expression of ZBRK1 represses transcriptional activity of the HIV-1 LTR, whereas the depletion of endogenous ZBRK1 leads to activation of the HIV-1 LTR. The repressor activity of ZBRK1 is required for TRIM28 binding. Furthermore, ZBRK1 is bound to the HIV-1 LTR in vivo. These results indicate that ZBRK1 could be involved in a potent intrinsic antiretroviral defense.
authors.affiliation: Department of Life and Environmental Sciences, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. hironori.nishitsuji@it-chiba.ac.jp
authors.forename: Hironori
authors.initials: H
authors.lastname: Nishitsuji
authors.forename: Makoto
authors.initials: M
authors.lastname: Abe
authors.forename: Reila
authors.initials: R
authors.lastname: Sawada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Takaku
mesh_terms: D015967:Gene Expression Regulation, Viral / Q000235:genetics; D020868:Gene Silencing; D057809:HEK293 Cells; D015497:HIV-1 / Q000235:genetics*; D006655:Histone Deacetylases / Q000378:metabolism; D006801:Humans; D012097:Repressor Proteins / Q000378:metabolism*; D020218:Response Elements / Q000235:genetics; D020079:Terminal Repeat Sequences / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics*; D000076183:Tripartite Motif-Containing Protein 28
chemical_list: D012097:Repressor Proteins; C422407:ZNF350 protein, human; C101116:TRIM28 protein, human; D000076183:Tripartite Motif-Containing Protein 28; D006655:Histone Deacetylases