title: Recombinant mycobacterial HSP65 in combination with incomplete Freund's adjuvant induced rat arthritis comparable with that induced by complete Freund's adjuvant.
abstract: Complete Freund's adjuvant (CFA) containing the inactivated mycobacterium has been conventionally used to induce typical arthritis in rats. In comparison, incomplete Freund's adjuvant (IFA), lacking the mycobacterium, can only induce less severe arthritis. However, the key components responsible for the arthritogenic effect of the whole mycobacterium are rarely known. Although mycobacterial heat-shock protein 65 (MHSP65) specific humoral and cellular immune responses were detected in rats with arthritis induced by CFA, MHSP65 alone cannot induce arthritis. In this study, we replaced the whole mycobacterium in CFA with recombinant MHSP65 (rMHSP65), prepared rMHSP65-IFA emulsion by mixing rMHSP65 and IFA, and investigated whether rMHSP65-IFA could induce arthritis in rats as CFA did. We found that intradermal injection of the rMHSP65-IFA emulsion induced arthritic lesions in testing animals to the same degree as those induced by CFA, manifested by severe swelling in hind paws, and synovial thickening, cartilage erosion and lymphocytes infiltration in ankle joints. Notably, the rMHSP65-IFA recipe also induced the production of anti-dsDNA and -rMHSP65 antibodies in rats. These results thus demonstrate that rMHSP65 can be used to substitute the inactivated mycobacteria in CFA to induce typical arthritis in rats.
authors.affiliation: Department of Molecular Biology, Norman Bethune Medical College, Jilin University, Changchun 130021, China. orangenesta@yahoo.com.cn
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yu
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Peiyin
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wu
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Di
authors.forename: Hongyu
authors.initials: H
authors.lastname: Jiang
authors.forename: Liying
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000843:Ankle Joint / Q000276:immunology; D000974:Antibodies, Antinuclear / Q000378:metabolism; D001169:Arthritis, Experimental / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D018834:Chaperonin 60 / Q000276:immunology*; D005620:Freund's Adjuvant / Q000276:immunology*; D006801:Humans; D008055:Lipids / Q000276:immunology*; D008297:Male; D023421:Models, Animal; D051381:Rats; D017208:Rats, Wistar; D013583:Synovial Membrane / Q000473:pathology
chemical_list: D000974:Antibodies, Antinuclear; D001426:Bacterial Proteins; D018834:Chaperonin 60; D008055:Lipids; C074119:heat-shock protein 65, Mycobacterium; C114843:incomplete Freund's adjuvant; D005620:Freund's Adjuvant