title: 2D-DIGE proteomic analysis of mesenchymal stem cell cultured on the elasticity-tunable hydrogels.
abstract: The present study focuses on mechanotransduction in mesenchymal stem cells (MSCs) in response to matrix elasticity. By using photocurable gelatinous gels with tunable stiffness, proteomic profiles of MSCs cultured on tissue culture plastic, soft (3 kPa) and stiff (52 kPa) matrices were deciphered using 2-dimensional differential in-gel analysis (2D-DIGE). The DIGE data, tied to immunofluorescence, indicated abundance and organization changes in the cytoskeletonal proteins as well as differential regulation of important signaling-related proteins, stress-responsing proteins and also proteins involved in collagen synthesis. The major CSK proteins including actin, tubulin and vimentin of the cells cultured on the gels were remarkably changed their expressions. Significant down-regulation of α-tubulin and β-actin can be observed on gel samples in comparison to the rigid tissue culture plates. The expression abundance of vimentin appeared to be highest in the MSCs cultured on hard gels. These results suggested that the substrate stiffness significantly affects expression balances in cytoskeletal proteins of MSCs with some implications to cellular tensegrity.
authors.affiliation: Institute for Materials Chemistry and Engineering, Division of Biomolecular Chemistry, Kyushu University, Fukuoka, Japan.
authors.forename: Thasaneeya
authors.initials: T
authors.lastname: Kuboki
authors.forename: Fahsai
authors.initials: F
authors.lastname: Kantawong
authors.forename: Richard
authors.initials: R
authors.lastname: Burchmore
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Dalby
authors.forename: Satoru
authors.initials: S
authors.lastname: Kidoaki
mesh_terms: D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004548:Elasticity; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D020100:Hydrogels / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D040901:Proteomics; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D020100:Hydrogels