title: Tepidimonas fonticaldi sp. nov., a slightly thermophilic betaproteobacterium isolated from a hot spring.
abstract: A slightly thermophilic bacterial strain, designated AT-A2(T), was isolated from a hot spring water sample taken from the Antun hot spring in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain AT-A2(T) were aerobic, Gram-negative, motile by a single polar flagellum and formed non-pigmented colonies. Growth occurred at 35-60 °C (optimum, 55 °C), with 0-1.0 % NaCl (optimum, 0.2 %) and at pH 7.0-9.0 (optimum, pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain AT-A2(T) belonged to the genus Tepidimonas and its closest neighbour was Tepidimonas thermarum AA-1(T) with a sequence similarity of 97.5 %. The predominant cellular fatty acids were C16 : 0 (40.2 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 20.1 %) and C17 : 0 cyclo (11.5 %). The major respiratory quinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminolipid and several uncharacterized phospholipids. The DNA G+C content of strain AT-A2(T) was 70.1 mol%. The mean level of DNA-DNA relatedness between strain AT-A2(T) and Tepidimonas thermarum AA-1(T) was 23.9 %. On the basis of the phylogenetic and phenotypic data, strain AT-A2(T) should be classified as representing a novel species, for which the name Tepidimonas fonticaldi sp. nov. is proposed. The type strain is AT-A2(T) ( = LMG 26746(T) = KCTC 23862(T) = BCRC 80391(T)).
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Hsing-Wei
authors.initials: HW
authors.lastname: Huang
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan, ROC.
authors.forename: Jo-Shu
authors.initials: JS
authors.lastname: Chang
authors.affiliation: Green Energy and Environment Research Laboratories, Natural Resources, Technology Division, Industrial Technology Research Institute, Hsinchu, Taiwan, ROC.
authors.forename: Yin-Lung
authors.initials: YL
authors.lastname: Han
authors.affiliation: Green Energy and Environment Research Laboratories, Natural Resources, Technology Division, Industrial Technology Research Institute, Hsinchu, Taiwan, ROC.
authors.forename: Tai-Rong
authors.initials: TR
authors.lastname: Guo
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D020562:Betaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D045482:Hot Springs / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013624:Taiwan; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S