title: Molecular characterisation of Anisakidae larvae from fish in Adriatic Sea.
abstract: In the present study, anisakids from: tuna (Thunnus thynnus) fattened in the Croatian farm in middle Adriatic Sea, three different feral fish species caught near tuna farm (Trachurus trachurus, Scomber japonicus and Oblada melanura) and fish marketed in Croatia (T. trachurus) were analysed by morphology and molecular methods. Larvae were identified to the species level by PCR restriction fragment length polymorphism and characterised by sequencing of nuclear (internal transcribed spacer) and mitochondrial (cytochrome c oxidase subunit 2) markers. The results revealed diverse Anisakidae community consisting of: Anisakis pegreffi, Anisakis simplex (s.s.), Anisakis typica and Hysterothylacium aduncum. This is the first report of A. typica in Adriatic Sea, and also the first record of this species in T. thynnus as host in Mediterranean Sea. Molecular identification of H. aduncum found in co-infection with Anisakis larvae type I expands our knowledge of the occurrence of these taxa in the Adriatic Sea. Zoonotic Anisakidae worms found in fish from the Adriatic Sea could represent a risk to acquire parasitic infection/allergies in Croatia.
authors.affiliation: Laboratory for Aquaculture and Pathology of Aquatic Organisms, Division for Marine and Environmental Research, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, Croatia. ivardic@irb.hr
authors.forename: I
authors.initials: I
authors.lastname: Vardić Smrzlić
authors.forename: D
authors.initials: D
authors.lastname: Valić
authors.forename: D
authors.initials: D
authors.lastname: Kapetanović
authors.forename: B
authors.initials: B
authors.lastname: Kurtović
authors.forename: E
authors.initials: E
authors.lastname: Teskeredžić
mesh_terms: D000818:Animals; D017163:Anisakis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017756:Aquaculture; D017523:Croatia; D016172:DNA Fingerprinting; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes / Q000469:parasitology*; D007814:Larva / Q000145:classification / Q000235:genetics; D008460:Meat / Q000469:parasitology*; D008522:Mediterranean Sea; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D003576:Electron Transport Complex IV