title: Gliadin as a measure of gluten in foods containing wheat, rye, and barley-enzyme immunoassay method based on a specific monoclonal antibody to the potentially celiac toxic amino acid prolamin sequences: collaborative study.
abstract: The Working Group on Prolamin Analysis and Toxicity (WGPAT) organized a collaborative study to confirm whether the two R5 antibody-based ELISA test kits are able to detect gliadin in the lower mg/kg (ppm) level. Twenty laboratories investigated 12 blind-coded samples, spiked and naturally contaminated, to show the possibility of determining traces of gliadin in heat-treated or nonheat-treated foods by ELISA. It was shown that very small amounts of gliadin (below 100 ppm) could be detected by ELISA with a reproducibility RSD(R) (37%) and a repeatability RSD, (27%) common for ELISA under these conditions. The recovery of gliadin from the spiked samples was between 84 and 109%, based on the results of all laboratories, including those with poor performance. No false positives were found by the method (P < or =0.05), but one negative sample was contaminated during the bakery process. It is recommended that the method be accepted by AOAC as Official First Action.
authors.affiliation: R-Biopharm AG, An der neuen Bergstrasse 17, 64297 Darmstadt, Germany. u.immer@r-biopharm.de
authors.forename: Ulrike
authors.initials: U
authors.lastname: Immer
authors.forename: Sigrid
authors.initials: S
authors.lastname: Haas-Lauterbach
mesh_terms: D000465:Algorithms; D000596:Amino Acids / Q000032:analysis*; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002446:Celiac Disease / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination; D005903:Gliadin / Q000032:analysis*; D005983:Glutens / Q000032:analysis*; D001467:Hordeum / Q000378:metabolism*; D007124:Immunoenzyme Techniques / Q000379:methods*; D055315:Prolamins / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012434:Secale / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D055315:Prolamins; D011933:Reagent Kits, Diagnostic; D005983:Glutens; D005903:Gliadin