title: Capture, isolation and release of cancer cells with aptamer-functionalized glass bead array.
abstract: Early detection and isolation of circulating tumor cells (CTC) can enable better prognosis for cancer patients. A Hele-Shaw device with aptamer functionalized glass beads is designed, modeled, and fabricated to efficiently isolate cancer cells from a cellular mixture. The glass beads are functionalized with anti-epidermal growth factor receptor (EGFR) aptamer and sit in ordered array of pits in polydimethylsiloxane (PDMS) channel. A PDMS encapsulation is then used to cover the channel and to flow through cell solution. The beads capture cancer cells from flowing solution depicting high selectivity. The cell-bound glass beads are then re-suspended from the device surface followed by the release of 92% cells from glass beads using combination of soft shaking and anti-sense RNA. This approach ensures that the cells remain in native state and undisturbed during capture, isolation and elution for post-analysis. The use of highly selective anti-EGFR aptamer with the glass beads in an array and subsequent release of cells with antisense molecules provide multiple levels of binding and release opportunities that can help in defining new classes of CTC enumeration devices.
authors.affiliation: Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wan
authors.forename: Yaling
authors.initials: Y
authors.lastname: Liu
authors.forename: Peter B
authors.initials: PB
authors.lastname: Allen
authors.forename: Waseem
authors.initials: W
authors.lastname: Asghar
authors.forename: M Arif Iftakher
authors.initials: MA
authors.lastname: Mahmood
authors.forename: Jifu
authors.initials: J
authors.lastname: Tan
authors.forename: Holli
authors.initials: H
authors.lastname: Duhon
authors.forename: Young-tae
authors.initials: YT
authors.lastname: Kim
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
authors.forename: Samir M
authors.initials: SM
authors.lastname: Iqbal
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001800:Blood Specimen Collection; D002469:Cell Separation / Q000295:instrumentation*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D066246:ErbB Receptors / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology; D005898:Glass / Q000737:chemistry*; D006801:Humans; D055595:Mechanical Phenomena; D008863:Microspheres; D008969:Molecular Sequence Data; D009360:Neoplastic Cells, Circulating / Q000473:pathology*
chemical_list: D052157:Aptamers, Nucleotide; D004129:Dimethylpolysiloxanes; C013830:baysilon; D066246:ErbB Receptors