title: Determining early adhesion of cells on polysaccharides/PCL surfaces by a quartz crystal microbalance.
abstract: The early adhesions of cells to various biopolymers are important to their growths and proliferations. Here, the adhesion of cells (e.g., fibroblasts) on the electrode of a quartz crystal microbalance (QCM) that was coated by PCL or PEG/PCL and further adsorbed by chitosan (CS) or CS/hyaluronic acid (HA) layers, was examined by cell-counting technique, QCM method and MTS assay under a serum-free condition for 3 h. The surfaces on electrodes of the QCM were confirmed to have been modified by measuring their contact angles, FT-IR spectra and the weights of biopolymers affected the frequency shifts of the QCM. Among tested surfaces on electrodes, the adhesion of fibroblasts on a HA/CS/PCL surface was the most (e.g., 3.08 × 10(5) cells/cm(2)) while that on a PEG/PCL surface was the least (e.g., 0.7 × 10(5) cells/cm(2)), as determined by cell-counting technique. The frequency shift and the mass of adhering fibroblasts on HA/CS/PCL electrodes were -3,537 ± 770 Hz and 3.78 ± 0.22 μg (n = 3), respectively, that were significantly exceeded those on other electrodes (-393 ± 58 Hz and 0.32 ± 0.06 μg, n = 3, respectively, for PEG/PCL electrodes). These results were consistent with cell-counting technique. Although MTS assay yielded similar results, it was less sensitive than the two aforementioned methods. In conclusion, modified electrodes of a QCM provide a convenient and sensitive method for examining the early adhesion of cells (e.g., 3 h) to biopolymer surfaces.
authors.affiliation: Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, 123 University Road, Section 3, Douliou, Yunlin, 64002, Taiwan, ROC. twchung@yuntech.edu.tw
authors.forename: Tze-Wen
authors.initials: TW
authors.lastname: Chung
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Tyan
authors.forename: Rong-Ho
authors.initials: RH
authors.lastname: Lee
authors.forename: Chia-Wei
authors.initials: CW
authors.lastname: Ho
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials; D001704:Biopolymers / Q000032:analysis; D002448:Cell Adhesion; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000032:analysis / Q000737:chemistry; D004566:Electrodes; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000032:analysis; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D020099:Coated Materials, Biocompatible; D011091:Polyesters; D011108:Polymers; D011134:Polysaccharides; C016240:polycaprolactone; D006820:Hyaluronic Acid; D048271:Chitosan