title: Cross-species amplification of microsatellites reveals incongruence in the molecular variation and taxonomic limits of the Pilosocereus aurisetus group (Cactaceae).
abstract: The Pilosocereus aurisetus group contains eight cactus species restricted to xeric habitats in eastern and central Brazil that have an archipelago-like distribution. In this study, 5-11 microsatellite markers previously designed for Pilosocereus machrisii were evaluated for cross-amplification and polymorphisms in ten populations from six species of the P. aurisetus group. The genotypic information was subsequently used to investigate the genetic relationships between the individuals, populations, and species analyzed. Only the Pmac101 locus failed to amplify in all of the six analyzed species, resulting in an 88 % success rate. The number of alleles per polymorphic locus ranged from 2 to 12, and the most successfully amplified loci showed at least one population with a larger number of alleles than were reported in the source species. The population relationships revealed clear genetic clustering in a neighbor-joining tree that was partially incongruent with the taxonomic limits between the P. aurisetus and P. machrisii species, a fact which parallels the problematic taxonomy of the P. aurisetus group. A Bayesian clustering analysis of the individual genotypes confirmed the observed taxonomic incongruence. These microsatellite markers provide a valuable resource for facilitating large-scale genetic studies on population structures, systematics and evolutionary history in this group.
authors.affiliation: Departamento de Biologia, Universidade Federal de São Carlos, Rodovia João Leme dos Santos km 110, Sorocaba, São Paulo 18052780, Brazil. emarsola@ufscar.br
authors.forename: Evandro M
authors.initials: EM
authors.lastname: Moraes
authors.forename: Manolo F
authors.initials: MF
authors.lastname: Perez
authors.forename: Mariana F
authors.initials: MF
authors.lastname: Téo
authors.forename: Daniela C
authors.initials: DC
authors.lastname: Zappi
authors.forename: Nigel P
authors.initials: NP
authors.lastname: Taylor
authors.forename: Marlon C
authors.initials: MC
authors.lastname: Machado
mesh_terms: D000483:Alleles; D001499:Bayes Theorem; D001938:Brazil; D029421:Cactaceae / Q000235:genetics*; D003433:Crosses, Genetic; D017931:DNA Primers / Q000737:chemistry; D018744:DNA, Plant / Q000378:metabolism; D014644:Genetic Variation; D005838:Genotype; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D018744:DNA, Plant