title: Real-time molecular imaging throughout the entire cell cycle by targeted plasmonic-enhanced Rayleigh/Raman spectroscopy.
abstract: Due to their strong enhancement of scattered light, plasmonic nanoparticles have been utilized for various biological and medical applications. Here, we describe a new technique, Targeted Plasmonic-Enhanced Single-Cell Rayleigh/Raman Spectroscopy, to monitor the molecular changes of any cell-component, such as the nucleus, during the different phases of its full cell cycle by simultaneously recording its Rayleigh images and Raman vibration spectra in real-time. The analysis of the observed Raman DNA and protein peaks allowed the different phases of the cell cycle to be identified. This technique could be used for disease diagnostics and potentially improve our understanding of the molecular mechanisms of cellular functions such as division, death, signaling, and drug action.
authors.affiliation: Laser Dynamics Laboratory, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-040, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Kang
authors.forename: Lauren A
authors.initials: LA
authors.lastname: Austin
authors.forename: Mostafa A
authors.initials: MA
authors.lastname: El-Sayed
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003199:Computer Systems; D004261:DNA Replication; D006046:Gold; D006801:Humans; D053768:Metal Nanoparticles; D057054:Molecular Imaging / Q000379:methods*; D036103:Nanotechnology; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D014732:Vibration
chemical_list: D006046:Gold