title: [Degradation of 14-3-3beta appeared in apoptosis cell induced by PrP106-126 polypeptide].
abstract: To investigate changes of 14-3-3beta from apoptosis induced by PrP106-126 polypeptide, HeLa cell was incubated with PrP106-126 for 4h or 8h. Nucleus changes and the expression of PARP were detected differently by Hoechst staining and Western blotting. Expressing of protein and mRNA from 14-3-3beta was determined by Western blotting and Real-time PCR. The results show that typical nucleus pyknosis and chip of apoptosis and degradation of PARP were induced by PrP106-126 peptide in HeLa cells. Degradation of 14-3-3beta appeared in apoptosis groups induced by PrP106-126 peptide. However, 14-3-3beta mRNA did not display any changes in apoptosis groups. This study indicated that degradation of antiapoptosis protein 143-3beta induced by PrP106-126 peptide may be one of pathogenesis mechanism of prion disease.
authors.affiliation: Inner Mongolia Medical College, Hollyhock 010110, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Song
authors.forename: Xing
authors.initials: X
authors.lastname: Gan
authors.forename: Yu
authors.initials: Y
authors.lastname: Cui
authors.forename: Chen
authors.initials: C
authors.lastname: Gao
authors.forename: Xiao-Zhen
authors.initials: XZ
authors.lastname: Bo
authors.forename: Jun
authors.initials: J
authors.lastname: Han
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology*; D011328:Prions / Q000494:pharmacology*; D059748:Proteolysis / Q000187:drug effects*
chemical_list: D048948:14-3-3 Proteins; D010446:Peptide Fragments; D011328:Prions; C082632:prion protein (106-126)