title: Mediastinal adipose tissue expresses a pathogenic profile of 11 β-hydroxysteroid dehydrogenase Type 1, glucocorticoid receptor, and CD68 in patients with coronary artery disease.
abstract: OBJECTIVE
  Cardiac visceral fat is accepted to be a new marker for cardiometabolic risk due to its association with increased cardiovascular risk factors. This study aimed to compare the expression of 11 beta hydroxysteroid dehydrogenases (11β-HSD)-1, glucocorticoid receptor (GCR), and CD68 in mediastinal and subcutaneous adipose tissues (MAT, and SAT, respectively) and to assess their possible relationships with the development of coronary artery disease (CAD).
  
  
  RESULTS
  Expression of 11β-HSD-1, GCR, and CD68 mRNA levels were measured by quantitative real-time polymerase chain reaction in MAT and SAT tissues of 37 patients undergoing coronary artery bypass grafting due to CAD (CAD group) and 19 non-CAD patients (controls) undergoing heart valve surgery. 11β-HSD-1 in MAT and SAT and GCR expression in MAT and SAT were found to be significantly increased in CAD group when compared with controls (P<.05, respectively). In CAD group, 11β-HSD-1 mRNA levels were found to be significantly higher in MAT compared to SAT (P<.05). CD68 mRNA levels were significantly higher in MAT of CAD group compared to controls (P<.05). Immunohistochemical analyses demonstrated the presence of CD68+ cells and increased 11β-HSD-1 expression in MAT of CAD group compared to SAT.
  
  
  CONCLUSIONS
  The present study demonstrate that the mediastinal fat exhibits a pathogenic mRNA profile of 11β-HSD-1, GCR, and CD68. The identification of 11β-HSD-1 expression within the mediastinal fat, along with increased GCR expressions and the presence of CD68+ cells highlight that MAT potentially contributes to the pathogenesis of CAD.
authors.affiliation: Department Growth-Development and Pediatric Endocrinology, Child Health Institute, Istanbul University, Istanbul, Turkey.
authors.forename: Fatmahan
authors.initials: F
authors.lastname: Atalar
authors.forename: Selcuk
authors.initials: S
authors.lastname: Gormez
authors.forename: Baris
authors.initials: B
authors.lastname: Caynak
authors.forename: Gokce
authors.initials: G
authors.lastname: Akan
authors.forename: Gamze
authors.initials: G
authors.lastname: Tanriverdi
authors.forename: Sema
authors.initials: S
authors.lastname: Bilgic-Gazioglu
authors.forename: Demet
authors.initials: D
authors.lastname: Gunay
authors.forename: Cihan
authors.initials: C
authors.lastname: Duran
authors.forename: Belhhan
authors.initials: B
authors.lastname: Akpinar
authors.forename: Ugur
authors.initials: U
authors.lastname: Ozbek
authors.forename: Ahmet Sevim
authors.initials: AS
authors.lastname: Buyukdevrim
authors.forename: Zeliha
authors.initials: Z
authors.lastname: Yazıcı
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000096:biosynthesis*; D015703:Antigens, CD / Q000096:biosynthesis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis*; D003324:Coronary Artery Disease / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D008297:Male; D008482:Mediastinum / Q000473:pathology; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis*; D000097382:CD68 Molecule
chemical_list: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; C067980:CD68 antigen, human; D000097382:CD68 Molecule