title: Diagnostic devices for isothermal nucleic acid amplification.
abstract: Since the development of the polymerase chain reaction (PCR) technique, genomic information has been retrievable from lesser amounts of DNA than previously possible. PCR-based amplifications require high-precision instruments to perform temperature cycling reactions; further, they are cumbersome for routine clinical use. However, the use of isothermal approaches can eliminate many complications associated with thermocycling. The application of diagnostic devices for isothermal DNA amplification has recently been studied extensively. In this paper, we describe the basic concepts of several isothermal amplification approaches and review recent progress in diagnostic device development.
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei 10617, Taiwan. ccchang.ibme@gmail.com
authors.forename: Chia-Chen
authors.initials: CC
authors.lastname: Chang
authors.forename: Chien-Cheng
authors.initials: CC
authors.lastname: Chen
authors.forename: Shih-Chung
authors.initials: SC
authors.lastname: Wei
authors.forename: Hui-Hsin
authors.initials: HH
authors.lastname: Lu
authors.forename: Yang-Hung
authors.initials: YH
authors.lastname: Liang
authors.forename: Chii-Wann
authors.initials: CW
authors.lastname: Lin
mesh_terms: D019719:Diagnostic Equipment; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation* / Q000379:methods*; D013696:Temperature