title: IGF-1 prevents oxidative stress induced-apoptosis in induced pluripotent stem cells which is mediated by microRNA-1.
abstract: Oxidative stress contributes to tissue injury and cell death during the development of various diseases. The present study aims at investigating whether oxidative stress triggered by the exposure to hydrogen peroxide (H(2)O(2)) can induce apoptosis of induced pluripotent stem cells (iPS cells) in a mechanism mediated by insulin-like growth factor (IGF-1) and microRNA-1 (miR-1). iPS cells treated with H(2)O(2) showed increases in miR-1 expression, mitochondria dysfunction, cytochrome-c release and apoptosis, Addition of IGF-1 into the iPS cell cultures reduced the H(2)O(2) cytotoxicity. Prediction algorithms showed that 3'-untranslated regions of IGF-1 gene as a target of miR-1. Moreover, miR-1 mimic, but not miR-1 mimic negative control, diminished the protective effect of IGF-1 on H(2)O(2)-induced mitochondrial dysfunction, cytochrome-c release and apoptosis in iPS cells. In conclusion, IGF-1 inhibits H(2)O(2)-induced mitochondrial dysfunction, cytochrome-c release and apoptosis. IGF-1's effect is, at least partially, regulated by miR-1 in iPS cells.
authors.affiliation: The Texas Heart Institute and Center for Cardiovascular and Atherosclerosis Research, Department of Internal Medicine, University of Texas Medical School at Houston, TX 77030, USA. yli@texasheart.org
authors.forename: Yangxin
authors.initials: Y
authors.lastname: Li
authors.forename: Harnath
authors.initials: H
authors.lastname: Shelat
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Geng
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017209:Apoptosis; D002478:Cells, Cultured; D045304:Cytochromes c / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D057026:Induced Pluripotent Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D028361:Mitochondrial Diseases / Q000378:metabolism; D018384:Oxidative Stress
chemical_list: D020413:3' Untranslated Regions; C544547:MIRN1 microRNA, human; D035683:MicroRNAs; D007334:Insulin-Like Growth Factor I; D045304:Cytochromes c; D006861:Hydrogen Peroxide