title: Salivary hormone measurement using LC/MS/MS: specific and patient-friendly tool for assessment of endocrine function.
abstract: Saliva has recently been attracting attention as a patient-friendly available bio-fluid and an alternative to serum/plasma for hormone tests. LC coupled with atmospheric pressure ionization-MS/MS, especially electrospray ionization (ESI)-MS/MS, has been recently valued as a highly specific method in the analysis of salivary hormones. In this article, LC/ESI-MS/MS assays for salivary hormones are overviewed according to the papers that have been published during the last 5 years. Practical derivatization to enhance the detectabilities of hormones in ESI-MS/MS is also discussed, because a major disadvantage of using saliva is low hormone concentrations.
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan. higashi@rs.tus.ac.jp
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Higashi
mesh_terms: D001274:Atmospheric Pressure; D002853:Chromatography, Liquid / Q000379:methods*; D019963:Clinical Chemistry Tests / Q000379:methods*; D004703:Endocrine System / Q000378:metabolism / Q000502:physiology*; D006728:Hormones / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D012463:Saliva / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D006728:Hormones