title: Glycogen synthase kinase is a regulator of the circadian clock of Neurospora crassa.
abstract: Timekeeping by circadian clocks relies upon precise adjustment of expression levels of clock proteins. Here we identify glycogen synthase kinase (GSK) as a novel and critical component of the circadian clock of Neurospora crassa that regulates the abundance of its core transcription factor white collar complex (WCC) on a post-transcriptional level. We show that GSK specifically binds and phosphorylates both subunits of the WCC. Reduced expression of GSK promotes an increased accumulation of WC-1, the limiting factor of the WCC, causing an acceleration of the circadian clock and a shorter free-running period.
authors.affiliation: Biochemistry Center, University of Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany.
authors.forename: Özgür
authors.initials: Ö
authors.lastname: Tataroğlu
authors.forename: Linda
authors.initials: L
authors.lastname: Lauinger
authors.forename: Gencer
authors.initials: G
authors.lastname: Sancar
authors.forename: Katharina
authors.initials: K
authors.lastname: Jakob
authors.forename: Michael
authors.initials: M
authors.lastname: Brunner
authors.forename: Axel C R
authors.initials: AC
authors.lastname: Diernfellner
mesh_terms: D057906:Circadian Clocks; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D055879:Gene Knock-In Techniques; D038341:Glycogen Synthase Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D046912:Multiprotein Complexes / Q000378:metabolism; D009492:Neurospora crassa / Q000201:enzymology* / Q000502:physiology; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D013172:Spores, Fungal / Q000201:enzymology; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D046912:Multiprotein Complexes; D014157:Transcription Factors; C105589:wc-1 protein, Neurospora crassa; D038341:Glycogen Synthase Kinases