title: Expression and secretion of Aspergillus fumigatus proteases are regulated in response to different protein substrates.
abstract: The ubiquitous filamentous fungus Aspergillus fumigatus secretes a number of allergens with protease activity and has been linked to a variety of allergic conditions such as Severe Asthma with Fungal Sensitization (SAFS) and Allergic Bronchopulmonary Aspergillosis (ABPA). However, it is unclear which allergen proteases are being secreted during fungal invasion and whether the local biological environment regulates their expression. Understanding the dynamic expression of allergen proteases during growth of A. fumigatus may lead to further characterisation of the pathogenesis of these disorders as well as improved standardisation in the commercial production of these allergens. Secretion of proteases during germination and early growth of A. fumigatus was investigated in response to various complex protein sources (pig lung homogenate, mucin or casein). Protease inhibitor studies demonstrated that A. fumigatus (AF293 strain) secretes predominately serine proteases during growth in pig lung based medium and mainly metalloproteases during growth in casein based medium but suppressed protease secretion in unmodified Vogel's minimal medium and secreted both types in mucin based medium. Analysis of gene transcription and protein identification by mass spectrometry showed that the matrix metalloprotease, Mep/Asp f 5 and the serine protease, Alp1/Asp f 13, were upregulated and secreted during growth in pig lung medium, whereas Alp1 was predominately expressed and secreted in mucin based medium. In casein medium, the matrix metalloprotease, Lap1, was also upregulated and secreted in addition to Mep and Alp1. These findings suggest that A. fumigatus is able to detect different complex proteins available as substrates in its environment and regulate protease secretion accordingly. There is a requirement for the standardisation of A. fumigatus allergen extracts used both in clinical diagnosis of A. fumigatus allergy and in research studies.
authors.affiliation: Respiratory Research Group, School of Translational Medicine, University of Manchester, Manchester M13 9PT, UK. edward.farnell@postgrad.manchester.ac.uk
authors.forename: Edward
authors.initials: E
authors.lastname: Farnell
authors.forename: Karine
authors.initials: K
authors.lastname: Rousseau
authors.forename: David J
authors.initials: DJ
authors.lastname: Thornton
authors.forename: Paul
authors.initials: P
authors.lastname: Bowyer
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Herrick
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000378:metabolism / Q000382:microbiology*; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism*; D013552:Swine
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D011506:Proteins; D010447:Peptide Hydrolases