title: [IL-12-induced expression of TRAIL enhances the cytotoxicity of NK cells against Jurkat cells].
abstract: OBJECTIVE
  To study the mechanism underlying the IL-12-induced cytotoxic function of NK cells to Jurkat cells.
  
  
  METHODS
  NK cells from peripheral blood mononuclear cells (PBMCs) were purified by magnetic sorting  and stimulated with or without IL-12. The expression of genes on IL-12-treated and non-IL-12-treated NK cells was analyzed by gene chips and the expression of cytolytic molecules was evaluated by flow cytometry.
  
  
  RESULTS
  Seventeen genes were up- (5/17) or down-regulated (12/17) on IL-12-treated NK cells compared with non-IL-12-treated NK cells (fold change≥10). IL-12-induced expression of TRAIL on NK cells mediated the cytotoxicity to Jurkat cells. The expression of TRAIL on subsets of CD56(+);CD16(+); and CD56(-);CD16(+); NK cells significantly increased after the stimulation with IL-12 and Jurkat cells expressed high level of TRAIL receptor 2 (TRAIL-R2). Importantly, the neutralizing mAbs against TRAIL (RIK-2) significantly inhibited the cytotoxicity of NK cells induced by IL-12.
  
  
  CONCLUSIONS
  The expression of TRAIL on human NK cells induced by IL-12 was one of the major mechanisms of cytotoxicity to Jurkat cells.
authors.affiliation: Sun Yat-sen University, Guangzhou, China. xiaoying_fu@yahoo.cn
authors.forename: Xiao-ying
authors.initials: XY
authors.lastname: Fu
authors.forename: Bin-yan
authors.initials: BY
authors.lastname: Yang
authors.forename: Jun-qi
authors.initials: JQ
authors.lastname: Huang
authors.forename: Xi-gui
authors.initials: XG
authors.lastname: Long
authors.forename: Chang-you
authors.initials: CY
authors.lastname: Wu
mesh_terms: D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology*; D019169:Jurkat Cells; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics / Q000502:physiology*
chemical_list: D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D018664:Interleukin-12