title: Local inflammation is associated with aortic thrombus formation in abdominal aortic aneurysms. Relationship to clinical risk factors.
abstract: Intraluminal thrombus formation in aortic abdominal aneurysms (AAA) is associated with adverse clinical prognosis. Interplay between coagulation and inflammation, characterised by leukocyte infiltration and cytokine production, has been implicated in AAA thrombus formation. We studied leukocyte (CD45+) content by flow cytometry in AAA thrombi from 27 patients undergoing surgical repair. Luminal parts of thrombi were leukocyte-rich, while abluminal segments showed low leukocyte content. CD66b+ granulocytes were the most prevalent, but their content was similar to blood. Monocytes (CD14+) and T cells (CD3+) were also abundant, while content of B lymphocytes (CD19+) and NK cells (CD56+CD16+) were low. Thrombi showed comparable content of CD14highCD16- monocytes and lower CD14highCD16+ and CD14dimCD16+, than blood. Monocytes were activated with high CD11b, CD11c and HLA-DR expression. Total T cell content was decreased in AAA thrombus compared to peripheral blood but CD8 and CD3+CD4-CD8- (double negative T cell) contents were increased in thrombi. CD4+ cells were lower but highly activated (high CD69, CD25 and HLA-DR). No differences in T regulatory (CD4+CD25+FoxP3+) cell or pro-atherogenic CD4+CD28null lymphocyte content were observed between thrombi and blood. Thrombus T cells expressed high levels of CCR5 receptor for chemokine RANTES, commonly released from activated platelets. Leukocyte or T cell content in thrombi was not correlated with aneurysm size. However, CD3+ content was significantly associated with smoking in multivariate analysis taking into account major risk factors for atherosclerosis. In conclusion, intraluminal AAA thrombi are highly inflamed, predominantly with granulocytes, CD14highCD16- monocytes and activated T lymphocytes. Smoking is associated with T cell infiltration in AAA intraluminal thrombi.
authors.affiliation: Translational Medicine Laboratory, Department of Internal and Agricultural Medicine, Jagiellonian University School of Medicine, J Dietl Hospital, Ul Skarbowa 1, 31-121 Cracow, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sagan
authors.forename: Wojciech
authors.initials: W
authors.lastname: Mrowiecki
authors.forename: Tomasz P
authors.initials: TP
authors.lastname: Mikolajczyk
authors.forename: Karol
authors.initials: K
authors.lastname: Urbanski
authors.forename: Mateusz
authors.initials: M
authors.lastname: Siedlinski
authors.forename: Ryszard
authors.initials: R
authors.lastname: Nosalski
authors.forename: Ryszard
authors.initials: R
authors.lastname: Korbut
authors.forename: Tomasz J
authors.initials: TJ
authors.lastname: Guzik
mesh_terms: D000368:Aged; D017544:Aortic Aneurysm, Abdominal / Q000097:blood / Q000150:complications* / Q000276:immunology / Q000473:pathology; D001025:Aortitis / Q000097:blood / Q000150:complications / Q000276:immunology / Q000473:pathology; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000150:complications / Q000276:immunology / Q000473:pathology; D007962:Leukocytes / Q000145:classification / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation; D008215:Lymphocytes, Null / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000473:pathology; D019713:Receptors, CCR5 / Q000378:metabolism; D012307:Risk Factors; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000473:pathology; D013927:Thrombosis / Q000097:blood / Q000209:etiology* / Q000276:immunology / Q000473:pathology
chemical_list: D019713:Receptors, CCR5