title: EROD activity and genotoxicity in the seabob shrimp Xiphopenaeus kroyeri exposed to benzo[a]pyrene (BaP) concentrations.
abstract: Seabob shrimp Xiphopenaeus kroyeri is a marine species that lives in shallow waters of coastal environments, often impacted by polycyclic aromatic hydrocarbons (PAH) pollution. In the present study, seabob shrimp were exposed for 96 h to benzo[a]pyrene (BaP) at the nominal concentrations of 100, 200, 400 and 800 microg-L(-1). Animals of the control groups were exposed either to clean water or to the BaP-carrier (DMSO). At the end of the exposures, muscle tissues were sampled for BaP uptake assessment and hepatopancreas and hemolymph for EROD enzyme activity and hemocytes DNA damage, respectively. EROD activity and DNA damage increased significantly as a function of BaP exposure concentrations. Significant correlations between BaP uptake and both EROD activity and DNA damage suggest that they can be used as suitable tools for integrated levels of study on the biomarkers of PAH exposure.
authors.affiliation: Laboratório de Ecofisiologia de Animais Marinhos, Departamento de Oceanografia Biológica, Instituto Oceanográfico, Universidade de São Paulo, Praça do Oceanográfico, 191 Cidade Universitária, CEP 05508-900, São Paulo, Brazil. arrocha@usp.br
authors.forename: Arthur José
authors.initials: AJ
authors.lastname: da Silva Rocha
authors.forename: Vicente
authors.initials: V
authors.lastname: Gomes
authors.forename: Maria José de Arruda Campos
authors.initials: MJ
authors.lastname: Rocha Passos
authors.forename: Fabio Matsu
authors.initials: FM
authors.lastname: Hasue
authors.forename: Thaís Cruz
authors.initials: TC
authors.lastname: Alves Santos
authors.forename: Márcia Caruso
authors.initials: MC
authors.lastname: Bícego
authors.forename: Satie
authors.initials: S
authors.lastname: Taniguchi
authors.forename: Phan
authors.initials: P
authors.lastname: Van Ngan
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D009153:Mutagens / Q000633:toxicity; D033561:Penaeidae / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D009153:Mutagens; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene; D019363:Cytochrome P-450 CYP1A1