title: HTLV-1-infected T cells contain a single integrated provirus in natural infection.
abstract: Human T lymphotropic virus type 1 (HTLV-1) appears to persist in the chronic phase of infection by driving oligoclonal proliferation of infected T cells. Our recent high-throughput sequencing study revealed a large number (often > 10(4)) of distinct proviral integration sites of HTLV-1 in each host that is greatly in excess of previous estimates. Here we use the highly sensitive, quantitative high-throughput sequencing protocol to show that circulating HTLV-1(+) clones in natural infection each contain a single integrated proviral copy. We conclude that a typical host possesses a large number of distinct HTLV-1-infected T-cell clones.
authors.affiliation: Department of Immunology, Wright Fleming Institute, Imperial College London, United Kingdom.
authors.forename: Lucy B
authors.initials: LB
authors.lastname: Cook
authors.forename: Aileen G
authors.initials: AG
authors.lastname: Rowan
authors.forename: Anat
authors.initials: A
authors.lastname: Melamed
authors.forename: Graham P
authors.initials: GP
authors.lastname: Taylor
authors.forename: Charles R M
authors.initials: CR
authors.lastname: Bangham
mesh_terms: D002874:Chromosome Mapping; D002877:Chromosomes, Human; D002999:Clone Cells; D004279:DNA, Viral / Q000235:genetics*; D005260:Female; D015490:HTLV-I Infections / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D059014:High-Throughput Nucleotide Sequencing; D015368:Human T-lymphotropic virus 1 / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011533:Proviruses / Q000235:genetics*; D012720:Severity of Illness Index; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D019562:Viral Load; D016662:Virus Integration / Q000235:genetics*
chemical_list: D004279:DNA, Viral