title: Enhancing effect of IMP on myosin and actin extraction from porcine meat.
abstract: We examined the effects of nucleoside monophosphates on the dissociation of actomyosin into myosin and actin. GMP was effective only among GMP, CMP, dTMP, and UMP. Hence we concluded that purine-based nucleoside monophosphates such as GMP, AMP, and IMP are effective, incorporating this with our previous results (Okitani A et al., Biosci. Biotechnol. Biochem., 72, 2005-2011 (2008)). Then we examined whether IMP enhances the extraction of myosin and actin as well as pyrophosphate (KPP), using homogenates of pork with 9 volumes of 0.3, 0.4, and 0.5 M NaCl solutions containing 0-36 mM IMP or 0-9 mM KPP. Maximum extractability, about 70% for both proteins, was attained by means of NaCl solutions containing 36 mM IMP. These values were comparable to the maximum values, about 90% for myosin and 50% for actin, attained by means of solutions containing 9 mM KPP. Hence we concluded that IMP enhances the extraction of myosin and actin from porcine meat.
authors.affiliation: Association of Meat Science and Technology Institute, 1-5-6 Ebisu, Tokyo 150-0013, Japan. nakamura@niku-kakou.or.jp
authors.forename: Yukinobu
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Koshiro
authors.initials: K
authors.lastname: Migita
authors.forename: Akihiro
authors.initials: A
authors.lastname: Okitani
authors.forename: Masanori
authors.initials: M
authors.lastname: Matsuishi
mesh_terms: D000199:Actins / Q000737:chemistry*; D000205:Actomyosin / Q000737:chemistry*; D000818:Animals; D011756:Diphosphates / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007291:Inosine Monophosphate / Q000737:chemistry*; D059625:Liquid-Liquid Extraction / Q000379:methods; D008460:Meat; D018482:Muscle, Skeletal / Q000737:chemistry*; D009218:Myosins / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013552:Swine
chemical_list: D000199:Actins; D011756:Diphosphates; D017680:Potassium Compounds; D007291:Inosine Monophosphate; D012965:Sodium Chloride; D000205:Actomyosin; C035389:tetrapotassium pyrophosphate; D009218:Myosins