title: Pneumatically assisted electrospray-ion mobility spectrometry for quantitative analysis of intact proteins.
abstract: In this work, quantitative analysis of intact proteins using ion mobility spectrometry (IMS) is introduced. For this purpose a pneumatically assisted electrospray ionization source with a new design was constructed. Liquid and nebulizer gas flow rates were optimized to achieve the highest response. The pneumatically assisted electrospray-IMS was used for quantitative analysis of insulin, bovine serum albumin (BSA) and human serum albumin (HSA). The analysis of proteins demonstrated that sensitivity of the pneumatically assisted electrospray-IMS is two to three times higher than that of conventional electrospray ionization (ESI) coupled to IMS. The linear dynamic ranges for insulin, BSA and HSA were found to be 1-75, 5-100 and 2-100 μg mL(-1) with detection limits of 0.3, 1 and 0.6 μg mL(-1), respectively. The relative standard deviations (RSD) for five replicate measurements of insulin, BSA, and HSA at 25 μg mL(-1) were recorded as 3, 4 and 1.5%, respectively. The proposed method can be considered as an alternative way for quantitative protein analysis.
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Arabzadeh
authors.forename: T
authors.initials: T
authors.lastname: Khayamian
mesh_terms: D000818:Animals; D002417:Cattle; D006801:Humans; D007328:Insulin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D007328:Insulin; D011506:Proteins; D012710:Serum Albumin, Bovine