title: A soy-based product fermented by Enterococcus faecium and Lactobacillus helveticus inhibits the development of murine breast adenocarcinoma.
abstract: OBJECTIVE
  Soy and its fermented products are considered functional foods. The study objective was to assess three functional food - a non-fermented soy product (NFP), fermented soy product (FSP), fermented soy product enriched with isoflavones (FI) - in terms of their ability to reduce the development of adenocarcinoma in mice, as well their ability on modulating immune system.
  
  
  METHODS
  It was observed tumor volume and to verify correlations with the immune system it was measured levels of the cytokines IL-1β and TNF-α produced by macrophages as well as IFN-γ produced by lymphocytes using ELISA test, and nitric oxide production by macrophages using Griess reagent.
  
  
  RESULTS
  All products showed immunological activity, but FSP showed the most effective tumor containment, resulting in smallest tumor volumes. FI animals expressed larger amounts of nitric oxide and IL-1β and exhibited larger tumor sizes than FSP and NFP animals.
  
  
  CONCLUSIONS
  The results suggested that the ingestion of FSP was most efficient in tumor containment, possibly due to a positive modulation of the immune system by when Enterococcus faecium and Lactobacillus helveticus are added to the soy product.
authors.affiliation: Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista-Rua Expedicionários do Brasil, n 1621, Araraquara, SP, Brazil. ferjuki@yahoo.com.br
authors.forename: Fernanda Lopes
authors.initials: FL
authors.lastname: Kinouchi
authors.forename: Danielle Cardoso Geraldo
authors.initials: DC
authors.lastname: Maia
authors.forename: Lívia Carolina
authors.initials: LC
authors.lastname: de Abreu Ribeiro
authors.forename: Marisa Campos Polesi
authors.initials: MC
authors.lastname: Placeres
authors.forename: Graciela Font
authors.initials: GF
authors.lastname: de Valdez
authors.forename: Lucas Luis
authors.initials: LL
authors.lastname: Colombo
authors.forename: Elizeu Antônio
authors.initials: EA
authors.lastname: Rossi
authors.forename: Iracilda Zeppone
authors.initials: IZ
authors.lastname: Carlos
mesh_terms: D000230:Adenocarcinoma / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism; D016984:Enterococcus faecium; D005260:Female; D005285:Fermentation; D007371:Interferon-gamma / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D052198:Lactobacillus helveticus; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D013025:Glycine max / Q000382:microbiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma