title: Magnetovibrio blakemorei gen. nov., sp. nov., a magnetotactic bacterium (Alphaproteobacteria: Rhodospirillaceae) isolated from a salt marsh.
abstract: A magnetotactic bacterium, designated strain MV-1(T), was isolated from sulfide-rich sediments in a salt marsh near Boston, MA, USA. Cells of strain MV-1(T) were Gram-negative, and vibrioid to helicoid in morphology. Cells were motile by means of a single polar flagellum. The cells appeared to display a transitional state between axial and polar magnetotaxis: cells swam in both directions, but generally had longer excursions in one direction than the other. Cells possessed a single chain of magnetosomes containing truncated hexaoctahedral crystals of magnetite, positioned along the long axis of the cell. Strain MV-1(T) was a microaerophile that was also capable of anaerobic growth on some nitrogen oxides. Salinities greater than 10 % seawater were required for growth. Strain MV-1(T) exhibited chemolithoautotrophic growth on thiosulfate and sulfide with oxygen as the terminal electron acceptor (microaerobic growth) and on thiosulfate using nitrous oxide (N2O) as the terminal electron acceptor (anaerobic growth). Chemo-organoautotrophic and methylotrophic growth was supported by formate under microaerobic conditions. Autotrophic growth occurred via the Calvin-Benson-Bassham cycle. Chemo-organoheterotrophic growth was supported by various organic acids and amino acids, under microaerobic and anaerobic conditions. Optimal growth occurred at pH 7.0 and 26-28 °C. The genome of strain MV-1(T) consisted of a single, circular chromosome, about 3.7 Mb in size, with a G+C content of 52.9-53.5 mol%.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MV-1(T) belongs to the family Rhodospirillaceae within the Alphaproteobacteria, but is not closely related to the genus Magnetospirillum. The name Magnetovibrio blakemorei gen. nov., sp. nov. is proposed for strain MV-1(T). The type strain of Magnetovibrio blakemorei is MV-1(T) ( = ATCC BAA-1436(T)  = DSM 18854(T)).
authors.affiliation: School of Life Sciences, University of Nevada at Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154, USA.
authors.forename: Dennis A
authors.initials: DA
authors.lastname: Bazylinski
authors.affiliation: School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Williams
authors.affiliation: CEA Cadarache/CNRS/Aix-Marseille Université, UMR7265 Service de Biologie Végétale et de Microbiologie Environnementale, Laboratoire de Bioénergétique Cellulaire, 13108 Saint Paul lez Durance, France.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Lefèvre
authors.affiliation: School of Life Sciences, University of Nevada at Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154, USA.
authors.forename: Denis
authors.initials: D
authors.lastname: Trubitsyn
authors.affiliation: Department of Natural Sciences, Hawaii Pacific University, 45-045 Kamehameha Highway, Kaneohe, HI 96744, USA.
authors.forename: Jiasong
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Molecular and Cellular Biology, College of Biological Science, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Terrence J
authors.initials: TJ
authors.lastname: Beveridge
authors.affiliation: Institute for Rock Magnetism, Department of Earth Sciences, University of Minnesota-Twin Cities, 310 Pillsbury Dr., SE, Minneapolis, MN 55455, USA.
authors.forename: Bruce M
authors.initials: BM
authors.lastname: Moskowitz
authors.affiliation: Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, UK.
authors.forename: Bruce
authors.initials: B
authors.lastname: Ward
authors.affiliation: School of Life Sciences, University of Nevada at Las Vegas, 4505 Maryland Parkway, Las Vegas, NV 89154, USA.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Schübbe
authors.affiliation: Life Technologies Corporation, 29851 Willow Creek Road, Eugene, OR 97402, USA.
authors.forename: Bradley L
authors.initials: BL
authors.lastname: Dubbels
authors.affiliation: United States Navy, Helseacombatron Two Three, San Diego, CA 92135, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Simpson
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D001900:Boston; D052837:Chemoautotrophic Growth; D004269:DNA, Bacterial / Q000235:genetics; D055944:Magnetosomes / Q000382:microbiology; D008969:Molecular Sequence Data; D010788:Photosynthesis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012244:Rhodospirillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride