title: Genome-wide in silico identification of GPI proteins in Mycosphaerella fijiensis and transcriptional analysis of two GPI-anchored β-1,3-glucanosyltransferases.
abstract: The hemibiotrophic fungus Mycosphaerella fijiensis is the causal agent of black Sigatoka (BS), the most devastating foliar disease in banana (Musa spp.) worldwide. Little is known about genes that are important during M. fijiensis-Musa sp. interaction. The fungal cell wall is an attractive area of study because it is essential for maintenance of cellular homeostasis and it is the most external structure in the fungal cell and therefore mediates the interaction of the pathogen with the host. In this manuscript we describe the in silico identification of glycosyl phosphatidylinositol-protein (GPI) family in M. fijiensis, and the analysis of two β-1,3-glucanosyltrans-ferases (Gas), selected by homology with fungal pathogenicity factors. Potential roles in pathogenesis were evaluated through analyzing expression during different stages of black Sigatoka disease, comparing expression data with BS symptoms and fungal biomass inside leaves. Real-time quantitative RT-PCR showed nearly constant expression of MfGAS1 with slightly increases (about threefold) in conidia and at speck-necrotrophic stage during banana-pathogen interaction. Conversely, MfGAS2 expression was increased during biotrophy (about seven times) and reached a maximum at speck (about 23 times) followed by a progressive decrease in next stages, suggesting an active role in M. fijiensis pathogenesis.
authors.affiliation: Unidad de biotecnología, Centro de Investigación Científica de Yucatán, Calle 43, No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, México.
authors.forename: Nuvia
authors.initials: N
authors.lastname: Kantún-Moreno
authors.forename: Roberto
authors.initials: R
authors.lastname: Vázquez-Euán
authors.forename: Miguel
authors.initials: M
authors.lastname: Tzec-Simá
authors.forename: Leticia
authors.initials: L
authors.lastname: Peraza-Echeverría
authors.forename: Rosa
authors.initials: R
authors.lastname: Grijalva-Arango
authors.forename: Cecilia
authors.initials: C
authors.lastname: Rodríguez-García
authors.forename: Andrew C
authors.initials: AC
authors.lastname: James
authors.forename: Jorge
authors.initials: J
authors.lastname: Ramírez-Prado
authors.forename: Ignacio
authors.initials: I
authors.lastname: Islas-Flores
authors.forename: Blondy
authors.initials: B
authors.lastname: Canto-Canché
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D002473:Cell Wall / Q000201:enzymology; D004271:DNA, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification; D058851:GPI-Linked Proteins / Q000235:genetics / Q000302:isolation & purification*; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal / Q000235:genetics*; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000235:genetics*; D017261:Glycosylphosphatidylinositols; D054884:Host-Pathogen Interactions; D005810:Multigene Family; D028521:Musa / Q000382:microbiology*; D025282:Mycelium; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D012331:RNA, Fungal / Q000235:genetics; D013172:Spores, Fungal; D014774:Virulence
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D012331:RNA, Fungal; C117939:1,3-beta-glucanosyltransferase; D004693:Glucan Endo-1,3-beta-D-Glucosidase