title: Hepatoprotective and antiviral properties of isochlorogenic acid A from Laggera alata against hepatitis B virus infection.
abstract: BACKGROUND
  The aim of this study was to determine the anti-hepatitis B effect of isochlorogenic acid A isolated from Laggera alata (Asteraceae), a traditional Chinese herbal medicine.
  
  
  METHODS
  The anti-hepatitis B activity of isochlorogenic acid A was evaluated by the D-galactosamine (D-GalN)-induced HL-7702 hepatocyte damage model and the HBV-transfected HepG2.2.15 cells.
  
  
  RESULTS
  Isochlorogenic acid A significantly improved HL-7702 hepatocyte viability and markedly inhibited the productions of HBsAg and HBeAg. The inhibitory rates of isochlorogenic acid A on the HBsAg and HBeAg expressions were 86.9% and 72.9%, respectively. In addition, isochlorogenic acid A declined markedly the content of hepatitis B virus covalently closed circular DNA (HBV cccDNA) and induced significantly the heme oxygenase-1 (HO-1) expression in HepG2.2.15 cells.
  
  
  CONCLUSIONS
  Isochlorogenic acid A was verified to possess the potent anti-hepatitis B activity. The anti-HBV target of isochlorogenic acid A is probably associated with blocking the translation step of the HBV replication. Overexpression of HO-1 may contribute to the anti-HBV activity of isochlorogenic acid A by reducing the stability of the HBV core protein and thus blocking the refill of nuclear HBV cccDNA. Additionally, the hepatoprotective effect of isochlorogenic acid A could be achieved by its antioxidative property and induction of HO-1.
authors.affiliation: Department of Pharmacy, Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
authors.forename: Bing-Jie
authors.initials: BJ
authors.lastname: Hao
authors.forename: Yi-Hang
authors.initials: YH
authors.lastname: Wu
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Wang
authors.forename: Shao-Qing
authors.initials: SQ
authors.lastname: Hu
authors.forename: Dana Jasmin
authors.initials: DJ
authors.lastname: Keil
authors.forename: Hua-Jun
authors.initials: HJ
authors.lastname: Hu
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Lou
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000956:Antigens, Viral / Q000032:analysis; D000998:Antiviral Agents / Q000494:pharmacology*; D019659:Asteraceae; D002470:Cell Survival / Q000187:drug effects; D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004279:DNA, Viral / Q000032:analysis; D051547:Heme Oxygenase-1 / Q000378:metabolism; D056945:Hep G2 Cells; D006509:Hepatitis B / Q000517:prevention & control; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D020011:Protective Agents / Q000494:pharmacology*
chemical_list: D000956:Antigens, Viral; D000998:Antiviral Agents; D004279:DNA, Viral; D020011:Protective Agents; C469310:isochlorogenic acid; D002726:Chlorogenic Acid; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1