title: Polydatin, a natural precursor of resveratrol, induces β-defensin production and reduces inflammatory response.
abstract: It is well known that human keratinocytes produce the anti-microbial peptide β-defensin 2. Its production is enhanced by pathogenic microorganisms or other environmental stressors. In this study, we evaluated the effect of resveratrol, a polyphenol found in several dietary source as grape seed, and its natural precursor, polydatin on heat-stressed human keratinocytes. By reverse transcription-polymerase chain reaction and enzyme-linked immunoadsorbent assay, we demonstrated that resveratrol used in combination with polydatin was able to modulate interleukin (IL)-6, IL-8 and tumor necrosis factor-alpha gene expression. In addition, our data show that resveratrol and polydatin increased the heat shock protein (Hsp)70B' gene expression, a Hsp that plays an important role in the cytoprotection and repair of cells and tissues. Worthy of note, polydatin used alone or in combination with resveratrol, increased the release of human β-defensin 2. These results highlighted the ability of polydatin and resveratrol to reinforce cytoprotective response in stress conditions and suggest their use in cosmetic or pharmaceutical preparations.
authors.affiliation: GLURES, Academic SPIN-OFF, Ca' Foscari University of Venice, Venice, Italy.
authors.forename: Giampietro
authors.initials: G
authors.lastname: Ravagnan
authors.forename: Anna
authors.initials: A
authors.lastname: De Filippis
authors.forename: Maria
authors.initials: M
authors.lastname: Cartenì
authors.forename: Salvatore
authors.initials: S
authors.lastname: De Maria
authors.forename: Valentina
authors.initials: V
authors.lastname: Cozza
authors.forename: Marcella
authors.initials: M
authors.lastname: Petrazzuolo
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Tufano
authors.forename: Giovanna
authors.initials: G
authors.lastname: Donnarumma
mesh_terms: D002460:Cell Line; D019610:Cytoprotection / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000378:metabolism; D018869:Heat-Shock Response; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism; D016209:Interleukin-8 / Q000096:biosynthesis / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism*; D000077185:Resveratrol; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013267:Stilbenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism; D023083:beta-Defensins / Q000096:biosynthesis* / Q000378:metabolism
chemical_list: D005960:Glucosides; D018840:HSP70 Heat-Shock Proteins; C544598:HSPA6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D023083:beta-Defensins; D000077185:Resveratrol; C058229:polydatin