title: Marginal vitamin B-6 deficiency decreases plasma (n-3) and (n-6) PUFA concentrations in healthy men and women.
abstract: Previous animal studies showed that severe vitamin B-6 deficiency altered fatty acid profiles of tissue lipids, often with an increase of linoleic acid and a decrease of arachidonic acid. However, little is known about the extent to which vitamin B-6 deficiency affects human fatty acid profiles. The aim of this study was to determine the effects of marginal vitamin B-6 deficiency on fatty acid profiles in plasma, erythrocytes, and peripheral blood mononuclear cells (PBMC) of healthy adults fed a 28-d, low-vitamin B-6 diet. Healthy participants (n = 23) received a 2-d, controlled, vitamin B-6-adequate diet followed by a 28-d, vitamin B-6-restricted diet to induce a marginal deficiency. Plasma HDL and LDL cholesterol concentrations, FFA concentrations, and erythrocyte and PBMC membrane fatty acid compositions did not significantly change from baseline after the 28-d restriction. Plasma total arachidonic acid, EPA, and DHA concentrations decreased from (mean ± SD) 548 ± 96 to 490 ± 94 μmol/L, 37 ± 13 to 32 ± 13 μmol/L, and 121 ± 28 to 109 ± 28 μmol/L [positive false discovery rate (pFDR) adjusted P < 0.05], respectively. The total (n-6):(n-3) PUFA ratio in plasma exhibited a minor increase from 15.4 ± 2.8 to 16.6 ± 3.1 (pFDR adjusted P < 0.05). These data indicate that short-term vitamin B-6 restriction decreases plasma (n-3) and (n-6) PUFA concentrations and tends to increase the plasma (n-6):(n-3) PUFA ratio. Such changes in blood lipids may be associated with the elevated risk of cardiovascular disease in vitamin B-6 insufficiency.
authors.affiliation: Food Science and Human Nutrition Department, Institute of Food and Agricultural Sciences, niversity of Florida, Gainesville, FL, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhao
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Lamers
authors.forename: Maria A
authors.initials: MA
authors.lastname: Ralat
authors.forename: Bonnie S
authors.initials: BS
authors.lastname: Coats
authors.forename: Yueh-Yun
authors.initials: YY
authors.lastname: Chi
authors.forename: Keith E
authors.initials: KE
authors.lastname: Muller
authors.forename: James R
authors.initials: JR
authors.lastname: Bain
authors.forename: Meena N
authors.initials: MN
authors.lastname: Shankar
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Newgard
authors.forename: Peter W
authors.initials: PW
authors.lastname: Stacpoole
authors.forename: Jesse F
authors.initials: JF
authors.lastname: Gregory
mesh_terms: D000328:Adult; D016718:Arachidonic Acid / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D004032:Diet; D004281:Docosahexaenoic Acids / Q000097:blood*; D015118:Eicosapentaenoic Acid / Q000097:blood*; D004910:Erythrocyte Membrane / Q000737:chemistry; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects; D043371:Fatty Acids, Omega-6 / Q000097:blood*; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000187:drug effects; D008297:Male; D011795:Surveys and Questionnaires; D025101:Vitamin B 6 / Q000008:administration & dosage; D026681:Vitamin B 6 Deficiency / Q000097:blood / Q000503:physiopathology*; D055815:Young Adult
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D043371:Fatty Acids, Omega-6; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D025101:Vitamin B 6; D015118:Eicosapentaenoic Acid