title: Fabrication of non-enzymatic biosensor based on metallic catalyst-TiO2 hollow sphere nanocomposite for determining biomolecules.
abstract: A PtRu@TiO2-hollow nanocomposite for the detection of biomolecules was synthesized by chemical reduction. First, poly(styrene-co-vinylphenylboronic acid), PSB, was prepared as a template (approximately 250 nm) by surfactant-free emulsion polymerization. Second, PSB/TiO2 core-shell spheres were prepared by sol-gel reaction. Finally, TiO2 hollow spheres (TiO2-H) were then formed after removing the PSB template by calcination at 450 degrees C under air atmosphere. To prepare the electrocatalyst, PtRu nanoparticles (NPs) were deposited onto the TiO2-H surface by chemical reduction. The prepared PtRu@TiO2-H nanocomposite was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), and elemental analysis. A non-enzymatic sensor was fabricated by depositing the as-prepared PtRu@TiO2-H nanocomposite on the surface of a glassy carbon electrode (GCE), which was prepared by a hand casting method with Nafion solution as a binder. The sensor was tested as a biomolecule sensor, especially for the detection of glucose and dopamine. The cyclic voltammograms (CV) obtained during the oxidation studies revealed that the PtRu@TiO2-H nanocomposite showed better catalytic function toward the oxidation of dopamine. The sensing range of the non-enzymatic sensor for glucose was 5.0-100 mM in a phosphate buffer. The results demonstrated the potential usefulness of this bimetallic@TiO2-H bifunctional catalyst for biosensor applications.
authors.affiliation: Department of Chemistry Hannam University, Daejeon 305-811, Republic of Korea.
authors.forename: Hai-Doo
authors.initials: HD
authors.lastname: Kwen
authors.forename: Hee-Soo
authors.initials: HS
authors.lastname: Yang
authors.forename: In-Ho
authors.initials: IH
authors.lastname: Lee
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Choi
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004298:Dopamine / Q000032:analysis*; D004798:Enzymes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005947:Glucose / Q000032:analysis*; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D014025:Titanium / Q000737:chemistry*
chemical_list: D004798:Enzymes; C009495:titanium dioxide; D014025:Titanium; D005947:Glucose; D004298:Dopamine