title: The clinical significance of reduced programmed cell death 5 expression             in human gastrointestinal stromal tumors.
abstract: Programmed cell death 5 (PDCD5) is a novel apoptosis-related gene with potent             antitumor effects which can interact with the histone acetyltransferase Tip60             and induce DNA damage-induced apoptosis. Reduced PDCD5 expression has been found             in a few types of human tumors and is also associated with the progression and             prognosis of the tumors. However, the expression status and clinical significance             of PDCD5 in gastrointestinal stromal tumors has not yet been analyzed. In our             study, we examined PDCD5 expression in 63 tumor samples of gastrointestinal stroma             at both mRNA and protein levels by RT-PCR, western blotting and immunohistochemistry.             We found that 57% (16/28) of the tumor samples had a decreased PDCD5 expression             at the mRNA level and 53% (35/66) of the samples were found to have decreased             PDCD5 expression at the protein level, whereas PDCD5 was highly expressed in all             adjacent normal gastrointestinal tissues at the mRNA or protein level. Moreover,             decreased PDCD5 expression was significantly associated with clinicopathological             characteristics including tumor size and mitosis. Our results suggest that PDCD5             expression plays a significant role in the malignant progression of human gastrointestinal             stromal tumors and may be a key inhibitory factor.
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Shandong University Qilu Hospital, Jinan, Shandong, PR China. gaofeijn@yahoo.com.cn
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Lingling
authors.initials: L
authors.lastname: Ding
authors.forename: Miaoqing
authors.initials: M
authors.lastname: Zhao
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Qu
authors.forename: Shanying
authors.initials: S
authors.lastname: Huang
authors.forename: Lining
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis / Q000235:genetics*; D004249:DNA Damage; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000235:genetics / Q000378:metabolism*; D046152:Gastrointestinal Stromal Tumors / Q000235:genetics* / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D009363:Neoplasm Proteins; C117283:PDCD5 protein, human; D012333:RNA, Messenger