title: The impact of water-floating and high-intensity exercise on rat's HPA axis and interleukins concentrations.
abstract: Our studies explore the changes of blood corticosterone (CORT), adrenocorticotropic hormone (ACTH), interleukin (IL)-1β, IL-2, IL-6 concentrations and the pituitary ACTH expression in rats after water floating in the presence or absence of following high-intensity exercise. The rats were randomly assigned into three groups. Group A served as control; Group B received 180 minutes water floating and psychological (fear) stimulation; Group C received the same treatment as Group B in addition and 120-minutes non-stop running. Compared to Group A, Group B showed a significant increase of IL-2 (19.91 ± 2.52 vs. 13.09 ± 3.13 ng/ml, P < 0.05), and IL-6 (0.18 ± 0.08 vs. 0.12 ± 0.05 ng/ml, P < 0.05); Group C demonstrated a significant increase of CORT (977.22 ± 207.36 ng/ml vs. 434.58 ± 110.45 ng/ml, P <0.01) and IL-1β (0.21 ± 0.04 vs. 0.16 ± 0.06 ng/ml, P < 0.05), IL-2 (20.29 ± 4.23 vs. 13.09 ± 3.13 ng/ml, P < 0.05), and IL-6 (0.19 ± 0.03 vs. 0.12 ± 0.05 ng/ml, P < 0.05) levels, and a significant decrease of ACTH (16.95 ± 5.46 vs. 22.96 ± 7.32 pg/ml, P = 0.03). Immunohistochemical staining showed the decreased number of pituitary ACTH-positive cells in both Groups B and C (P < 0.05) as compared to Group A. These results have lead us to believe that acute psychological stress can activate the pituitary-adrenal axis and lead to elevation of serum IL-2, IL-6 concentrations. Combined with high-intensity exercise, it can result in the increase of serum CORT, IL-1β, IL-2, IL-6 levels, and the suppression of ACTH.
authors.affiliation: Department of Endocrinology, Jingling Hospital, Nanjing China. jianwang_11@yahoo.com
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Guohong
authors.initials: G
authors.lastname: Wang
authors.forename: Lanping
authors.initials: L
authors.lastname: Hu
authors.forename: Jiaging
authors.initials: J
authors.lastname: Shao
authors.forename: Ping
authors.initials: P
authors.lastname: Gu
authors.forename: Hong
authors.initials: H
authors.lastname: Du
authors.forename: Yangt
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D003345:Corticosterone / Q000097:blood; D005239:Fear / Q000502:physiology; D007030:Hypothalamo-Hypophyseal System / Q000502:physiology*; D053583:Interleukin-1beta / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D007378:Interleukins / Q000097:blood* / Q000276:immunology; D008297:Male; D010805:Physical Conditioning, Animal / Q000502:physiology*; D005082:Physical Exertion / Q000276:immunology / Q000502:physiology*; D010902:Pituitary Gland / Q000378:metabolism; D010913:Pituitary-Adrenal System / Q000502:physiology*; D051381:Rats; D013312:Stress, Physiological / Q000276:immunology / Q000502:physiology; D013550:Swimming / Q000502:physiology
chemical_list: D053583:Interleukin-1beta; D007376:Interleukin-2; D015850:Interleukin-6; D007378:Interleukins; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone