title: Anti-inflammatory effects of Hylomecon hylomeconoides in RAW 264.7 cells.
abstract: OBJECTIVE
  Papaveraceae serve as a rich source of various alkaloids which have anti-inflammatory effect.
  
  
  METHODS
  In this study, we investigated the effect of Hylomecon hylomeconoides ethanol extract (HHE) on lipopolysaccharide (LPS)-induced NO and interleukin-6 (IL-6) production in RAW 264.7 cells.
  
  
  RESULTS
  HHE inhibited LPS-induced NO and IL-6 production. Moreover, HHE suppressed the phosphorylation of ERK1/2 and p38 in LPS-induced RAW 264.7 in a dose-dependent manner. Furthermore, major constituents, dihydrosanguinarine and 6-methoxydihydrosanguinarine, of the chloroform-soluble extract were analyzed.
  
  
  CONCLUSIONS
  Taken together, the results of this study indicate that the anti-inflammatory effects of HHE may occur via the inhibition of NO and IL-6 expression through the down-regulation of MAP kinase (ERK1/2, p38) phosphorylation in RAW 264.7 cells.
authors.affiliation: Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Wonkwang, Republic of Korea.
authors.forename: H S
authors.initials: HS
authors.lastname: Chae
authors.forename: O H
authors.initials: OH
authors.lastname: Kang
authors.forename: J H
authors.initials: JH
authors.lastname: Keum
authors.forename: S B
authors.initials: SB
authors.lastname: Kim
authors.forename: S H
authors.initials: SH
authors.lastname: Mun
authors.forename: Y S
authors.initials: YS
authors.lastname: Seo
authors.forename: M R
authors.initials: MR
authors.lastname: Kim
authors.forename: J G
authors.initials: JG
authors.lastname: Choi
authors.forename: D W
authors.initials: DW
authors.lastname: Shin
authors.forename: Y C
authors.initials: YC
authors.lastname: Oh
authors.forename: J Y
authors.initials: JY
authors.lastname: Ma
authors.forename: D Y
authors.initials: DY
authors.lastname: Kwon
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy* / Q000503:physiopathology; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D028101:Papaveraceae / Q000737:chemistry*; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010936:Plant Extracts; D009569:Nitric Oxide