title: Simultaneous determination of iridoid glycosides, phenethylalcohol glycosides and furfural derivatives in Rehmanniae Radix by high performance liquid chromatography coupled with triple-quadrupole mass spectrometry.
abstract: In this study, a sensitive and selective method for simultaneously quantifying eight major components (four iridoid glycosides, three phenethylalcohol glycosides and one furfural derivative) of Rehmanniae Radix by high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (HPLC-TQ-MS) was developed. The sample preparation was executed using an optimised ultrasonic method with complete extraction efficiencies of eight analytes. For mass spectrometry, selected ion recording (SIR) scan mode was used to improve the sensitivity and selectivity. The established method was validated in terms of linearity, sensitivity, precision, accuracy and stability, and successfully applied to determine the contents of the eight analytes in different batches of raw and processed Rehmanniae Radix, which confirmed that the established method was reliable and useful for "holistic" quality evaluation of Rehmanniae Radix. The quantitative results indicated that the quality of commercial raw or processed Rehmanniae Radix was remarkably inconsistent.
authors.affiliation: Department of Pharmaceutical Analysis & Metabolomics and Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Province Academy of Traditional Chinese Medicine, No. 100 Shizi Street, Hongshan Road, Nanjing 210028, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Ling-Ying
authors.initials: LY
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.forename: Jin-Di
authors.initials: JD
authors.lastname: Xu
authors.forename: Soren Rosendal
authors.initials: SR
authors.lastname: Jensen
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Jia
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Zhang
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005662:Furaldehyde / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D057889:Iridoid Glycosides / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D032267:Rehmannia / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D057889:Iridoid Glycosides; D005662:Furaldehyde