title: Scale-up of human embryonic stem cell culture using a hollow fibre bioreactor.
abstract: The commercialisation of human embryonic stem cell derived cell therapies for large patient populations is reliant on both minimising expensive and variable manual-handling methods whilst realising economies of scale. The Quantum Cell Expansion System, a hollow fibre bioreactor (Terumo BCT), was used in a pilot study to expand 60 million human embryonic stem cells to 708 million cells. Further improvements can be expected with optimisation of media flow rates throughout the run to better control the cellular microenvironment. High levels of pluripotency marker expression were maintained on the bioreactor, with 97.7 % of cells expressing SSEA-4 when harvested.
authors.affiliation: The Advanced Centre for Biochemical Engineering, University College London, London, UK. i.roberts@ucl.ac.uk
authors.forename: Iwan
authors.initials: I
authors.lastname: Roberts
authors.forename: Stefano
authors.initials: S
authors.lastname: Baila
authors.forename: R Brent
authors.initials: RB
authors.lastname: Rice
authors.forename: Michiel Etienne
authors.initials: ME
authors.lastname: Janssens
authors.forename: Kim
authors.initials: K
authors.lastname: Nguyen
authors.forename: Nathalie
authors.initials: N
authors.lastname: Moens
authors.forename: Ludmila
authors.initials: L
authors.lastname: Ruban
authors.forename: Diana
authors.initials: D
authors.lastname: Hernandez
authors.forename: Pete
authors.initials: P
authors.lastname: Coffey
authors.forename: Chris
authors.initials: C
authors.lastname: Mason
mesh_terms: D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods*; D053595:Embryonic Stem Cells / Q000502:physiology*; D015870:Gene Expression; D006801:Humans; D055746:Stage-Specific Embryonic Antigens / Q000096:biosynthesis
chemical_list: D055746:Stage-Specific Embryonic Antigens; C066584:stage-specific embryonic antigen-4