title: [Impact of anti-OX-LDL antibodies on   CD36 mRNA expression in monocytes].
abstract: OBJECTIVE
  To investigate the impact of antibodies to oxidized low-density lipoprotein (OX-LDL) on CD36 mRNA expression in monocytes and explore the mechanism underlying the impact on the formation of foam cells.
  
  
  METHODS
  U937 cells and the monocytes of New Zealand rabbit were respectively cultured in vitro and divided into 4 groups: the control group (cultured in nutrient medium of RPMI1640), the OX-LDL group (with additional OX-LDL of 50 μg/L in nutrient medium), the OX-LDL+Ab-OX-LDL group (with additional OX-LDL of 50 μg/L and Ab-OX-LDL of 100 μg/L in nutrient medium) and the Ab-OX-LDL group (with additional Ab-OX-LDL of 100 μg/L in nutrient medium). After 24-hour culture, the expression of CD36 mRNA was detected by semi-quantitative RT-PCR.
  
  
  RESULTS
  The expression of CD36 mRNA, either in the OX-LDL group or in the OX-LDL+Ab-OX-LDL group, was higher than that in the control group. After intervened by Ab-OX-LDL, the expression was respectively down-regulated by 64.80% in U937 cells and 35.18% in the monocytes of rabbit, which was statistically significant between the two species.
  
  
  CONCLUSIONS
  Antibodies to OX-LDL could negatively regulate the expression of CD36 mRNA in monocytes, and prevent monocyte in taking OX-LDL through the pathway of antigen CD36.
authors.affiliation: Department of Cardiology, Nanjing General Hospital of Nanjing Military Area Command, Nanjing, China. wangleiyes@sina.com.cn
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jian-bin
authors.initials: JB
authors.lastname: Gong
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Qi-gao
authors.initials: QG
authors.lastname: Zhang
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D018955:CD36 Antigens / Q000235:genetics* / Q000502:physiology; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D006801:Humans; D008077:Lipoproteins, LDL / Q000276:immunology*; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D020298:U937 Cells
chemical_list: D000906:Antibodies; D018955:CD36 Antigens; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; C099252:oxidized low density lipoprotein