title: High levels of immunoglobulin E autoantibody to 14-3-3 epsilon protein correlate with protection against severe Plasmodium falciparum malaria.
abstract: Plasmodium falciparum infection generally induces elevated total plasma levels of immunoglobulins, some of which recognize self- or parasite-specific antigens. To our knowledge, we are the first to report high levels of functional immunoglobulin E (IgE) autoantibodies recognizing brain 14-3-3 protein ε in asymptomatic P. falciparum malaria. 14-3-3 ε protein belongs to a family of proteins that binds to CD81, a member of the tetraspanin superfamily elicited in hepatocyte invasion by sporozoites. Levels of expression of 14-3-3 ε protein were found to be increased in vivo and in vitro during Plasmodium yoelii and P. falciparum intrahepatic development. Collectively, these results indicate that self-reactive IgE is produced during malaria. In addition, the negative correlation between levels of self-reactive IgE to 14-3-3 ε protein and parasitemia in asymptomatic malaria due to P. falciparum supports a role for these IgE molecules in defense mechanisms, probably by interfering with development of liver-stage parasites through the CD81 pathway.
authors.affiliation: Instituto Gulbenkian de Ciência, LEA CNRS-IGC, Oeiras,  Portugal.
authors.forename: Joana
authors.initials: J
authors.lastname: Duarte
authors.forename: Fabien
authors.initials: F
authors.lastname: Herbert
authors.forename: Vincent
authors.initials: V
authors.lastname: Guiyedi
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Franetich
authors.forename: Jacques
authors.initials: J
authors.lastname: Roland
authors.forename: Pierre-André
authors.initials: PA
authors.lastname: Cazenave
authors.forename: Dominique
authors.initials: D
authors.lastname: Mazier
authors.forename: Maryvonne
authors.initials: M
authors.lastname: Kombila
authors.forename: Constantin
authors.initials: C
authors.lastname: Fesel
authors.forename: Sylviane
authors.initials: S
authors.lastname: Pied
mesh_terms: D048948:14-3-3 Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000852:Anopheles / Q000469:parasitology; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood*; D007223:Infant; D008099:Liver / Q000469:parasitology; D016778:Malaria, Falciparum / Q000276:immunology* / Q000473:pathology; D010963:Plasmodium falciparum / Q000276:immunology / Q000502:physiology; D010967:Plasmodium yoelii / Q000276:immunology / Q000502:physiology
chemical_list: D048948:14-3-3 Proteins; D001323:Autoantibodies; D001324:Autoantigens; C482983:YWHAE protein, human; D007073:Immunoglobulin E