title: Microsatellite typing of Aspergillus flavus from clinical and environmental avian isolates.
abstract: Aspergillosis is one of the most common causes of death in captive birds. Aspergillus fumigatus accounts for approximately 95 % of aspergillosis cases and Aspergillus flavus is the second most frequent organism associated with avian infections. In the present study, the fungi were grown from avian clinical samples (post-mortem lung material) and environmental samples (eggs, food and litter). Microsatellite markers were used to type seven clinical avian isolates and 22 environmental isolates of A. flavus. A. flavus was the only species (28 % prevalence) detected in the avian clinical isolates, whereas this species ranked third (19 %) after members of the genera Penicillium (39 %) and Cladosporium (21 %) in the environmental samples. Upon microsatellite analysis, five to eight distinct alleles were detected for each marker. The marker with the highest discriminatory power had eight alleles and a 0.852 D value. The combination of all six markers yielded a 0.991 D value with 25 distinct genotypes. One clinical avian isolate (lung biopsy) and one environmental isolate (egg) shared the same genotype. Microsatellite typing of A. flavus grown from avian and environmental samples displayed an excellent discriminatory power and 100 % reproducibility. This study showed a clustering of clinical and environmental isolates, which were clearly separated. Based upon these results, aspergillosis in birds may be induced by a great diversity of isolates.
authors.affiliation: Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Rue Magida Boulila, 3029 Sfax, Tunisia.
authors.forename: Inès
authors.initials: I
authors.lastname: Hadrich
authors.affiliation: Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Rue Magida Boulila, 3029 Sfax, Tunisia.
authors.forename: Inès
authors.initials: I
authors.lastname: Drira
authors.affiliation: Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Rue Magida Boulila, 3029 Sfax, Tunisia.
authors.forename: Sourour
authors.initials: S
authors.lastname: Neji
authors.affiliation: Service du Laboratoire, CHU Hèdi-Chaker, 3029 Sfax, Tunisia.
authors.forename: Nedia
authors.initials: N
authors.lastname: Mahfoud
authors.affiliation: Aix-Marseille Université, IP-TPT UMR MD3, 13885 Marseille, France.|Parasitology & Mycology, CHU Timone-Adultes, Assistance Publique-Hôpitaux de Marseille, 13005 Marseille, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Ranque
authors.affiliation: Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Rue Magida Boulila, 3029 Sfax, Tunisia.
authors.forename: Fattouma
authors.initials: F
authors.lastname: Makni
authors.affiliation: Laboratoire de Biologie Moléculaire Parasitaire et Fongique, Faculté de Médecine de Sfax, Rue Magida Boulila, 3029 Sfax, Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Ayadi
mesh_terms: D000483:Alleles; D000818:Animals; D001231:Aspergillus flavus / Q000145:classification / Q000235:genetics*; D001715:Bird Diseases / Q000382:microbiology*; D002645:Chickens; D004271:DNA, Fungal / Q000235:genetics*; D004528:Egg Shell / Q000382:microbiology; D004783:Environmental Microbiology; D005516:Food Microbiology; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D010063:Ovum / Q000382:microbiology; D010802:Phylogeny; D055732:Pulmonary Aspergillosis / Q000382:microbiology / Q000662:veterinary*
chemical_list: D004271:DNA, Fungal