title: Correlation of deoxynivalenol and fumonisin concentration determined in maize by ELISA methods.
abstract: Maize (Zea mays) is one of the most common crops in Croatia that is often contaminated with mycotoxins. The enzyme linked immunosorbent assay (ELISA) method as a screening immunological method has become very popular in the determination of mycotoxins contamination of cereals, as it has many advantages such as a rapid and simple determination of their concentration. In this present study, 40 maize samples collected from animal feed factories were analyzed for the concentration of deoxynivalenol (DON) and fumonisin (FUM) using the validated ELISA method of two different kit manufacturers to investigate the correlation of the determined mycotoxins concentration. The correlation coefficients for the concentration of DON and FUM, obtained using different kit manufacturers, were r = 0.9698 and r = 0.9303, respectively, yielding a high correlation between the applied ELISA methods. Concerning the legislation, in one maize sample, the DON concentration higher than the maximum recommended value was determined (16.24 mg/kg), whereas the FUM concentration in all the analyzed samples, without exception, were in accordance with recommended values.
authors.affiliation: Department of Veterinary Public Health, Croatian Veterinary Institute, Zagreb, Croatia. pleadin@veinst.hr
authors.forename: Jelka
authors.initials: J
authors.lastname: Pleadin
authors.forename: Nina
authors.initials: N
authors.lastname: Perši
authors.forename: Manuela
authors.initials: M
authors.lastname: Zadravec
authors.forename: Marijana
authors.initials: M
authors.lastname: Sokolović
authors.forename: Ana
authors.initials: A
authors.lastname: Vulić
authors.forename: Vesna
authors.initials: V
authors.lastname: Jaki
authors.forename: Mario
authors.initials: M
authors.lastname: Mitak
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D037341:Fumonisins / Q000032:analysis*; D015203:Reproducibility of Results; D014255:Trichothecenes / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D037341:Fumonisins; D014255:Trichothecenes; C007262:deoxynivalenol