title: Global gene deletion analysis exploring yeast filamentous growth.
abstract: The dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and introduced them into a filamentous S. cerevisiae strain, Σ1278b. We identified genes involved in morphologically distinct forms of filamentation: haploid invasive growth, biofilm formation, and diploid pseudohyphal growth. Unique genes appear to underlie each program, but we also found core genes with general roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C. albicans.
authors.affiliation: Banting and Best Department of Medical Research, University of Toronto, Toronto, ON M5S 3E1, Canada.
authors.forename: Owen
authors.initials: O
authors.lastname: Ryan
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Shapiro
authors.forename: Christoph F
authors.initials: CF
authors.lastname: Kurat
authors.forename: David
authors.initials: D
authors.lastname: Mayhew
authors.forename: Anastasia
authors.initials: A
authors.lastname: Baryshnikova
authors.forename: Brian
authors.initials: B
authors.lastname: Chin
authors.forename: Zhen-Yuan
authors.initials: ZY
authors.lastname: Lin
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Cox
authors.forename: Frederick
authors.initials: F
authors.lastname: Vizeacoumar
authors.forename: Doris
authors.initials: D
authors.lastname: Cheung
authors.forename: Sondra
authors.initials: S
authors.lastname: Bahr
authors.forename: Kyle
authors.initials: K
authors.lastname: Tsui
authors.forename: Faiza
authors.initials: F
authors.lastname: Tebbji
authors.forename: Adnane
authors.initials: A
authors.lastname: Sellam
authors.forename: Fabian
authors.initials: F
authors.lastname: Istel
authors.forename: Tobias
authors.initials: T
authors.lastname: Schwarzmüller
authors.forename: Todd B
authors.initials: TB
authors.lastname: Reynolds
authors.forename: Karl
authors.initials: K
authors.lastname: Kuchler
authors.forename: David K
authors.initials: DK
authors.lastname: Gifford
authors.forename: Malcolm
authors.initials: M
authors.lastname: Whiteway
authors.forename: Guri
authors.initials: G
authors.lastname: Giaever
authors.forename: Corey
authors.initials: C
authors.lastname: Nislow
authors.forename: Michael
authors.initials: M
authors.lastname: Costanzo
authors.forename: Anne-Claude
authors.initials: AC
authors.lastname: Gingras
authors.forename: Robi David
authors.initials: RD
authors.lastname: Mitra
authors.forename: Brenda
authors.initials: B
authors.lastname: Andrews
authors.forename: Gerald R
authors.initials: GR
authors.lastname: Fink
authors.forename: Leah E
authors.initials: LE
authors.lastname: Cowen
authors.forename: Charles
authors.initials: C
authors.lastname: Boone
mesh_terms: D000483:Alleles; D018441:Biofilms / Q000254:growth & development; D002176:Candida albicans / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D004252:DNA Mutational Analysis; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D025301:Hyphae / Q000235:genetics / Q000254:growth & development; D009687:Nuclear Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics* / Q000254:growth & development*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: C100940:FLO8 protein, S cerevisaie; C109358:MSS11 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors