title: Identification, cDNA cloning and heterologous expression of a hyaluronidase from the tarantula Brachypelma vagans venom.
abstract: Hyaluronidases (Hyal) present in the venom of poisonous animals have been considered as "spreading factors" that facilitate a fast penetration of the venom in the prey. We have found that hyaluronidase from the tarantula Brachypelma vagans venom (BvHyal) displays a substrate-specific Hyal activity against hyaluronan. By using a combined strategy based on peptide sequencing and RT-PCR, we have cloned a BvHyal cDNA. Active recombinant BvHyal was efficiently expressed in a baculovirus system in insect cell.
authors.affiliation: Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mor. 62250, Mexico.
authors.forename: Herlinda
authors.initials: H
authors.lastname: Clement
authors.forename: Alejandro
authors.initials: A
authors.lastname: Olvera
authors.forename: Mabel
authors.initials: M
authors.lastname: Rodríguez
authors.forename: Fernando
authors.initials: F
authors.lastname: Zamudio
authors.forename: Laura A
authors.initials: LA
authors.lastname: Palomares
authors.forename: Lourival D
authors.initials: LD
authors.lastname: Possani
authors.forename: George V
authors.initials: GV
authors.lastname: Odell
authors.forename: Alejandro
authors.initials: A
authors.lastname: Alagón
authors.forename: Rosana
authors.initials: R
authors.lastname: Sánchez-López
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D003001:Cloning, Molecular; D006821:Hyaluronoglucosaminidase / Q000737:chemistry / Q000235:genetics*; D007313:Insecta; D011994:Recombinant Proteins / Q000096:biosynthesis; D013111:Spider Venoms / Q000201:enzymology*
chemical_list: D011994:Recombinant Proteins; D013111:Spider Venoms; D006821:Hyaluronoglucosaminidase