title: Improved method for the solid-phase synthesis of oligoribonucleotide 5'-triphosphates.
abstract: We have developed an improved solid-phase method for the synthesis of 5'-triphosphates (5'-TPs) of oligoribonucleotides. The method is based on the use of salicyl phosphorochloridite as the phosphitylating reagent and the improvement is characterized by the use of the highly reactive pyrophosphorylating reagent tris(tetra-n-butylammonium) hydrogen pyrophosphate instead of the conventional tri-n-butylammonium salt for the nucleophilic substitution reaction to form the cyclic ester intermediate. The improved method can be used to generate oligoribonucleotide 5'-TPs efficiently and reproducibly.
authors.affiliation: Discovery Research Laboratories, Nippon Shinyaku Co., Ltd., 3-14-1 Sakura, Tsukuba, Ibaraki 305-0003, Japan. s.nagata@po.nippon-shinyaku.co.jp
authors.forename: Seigo
authors.initials: S
authors.lastname: Nagata
authors.forename: Kazuchika
authors.initials: K
authors.lastname: Takagaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Wada
mesh_terms: D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D010766:Phosphorylation; D011122:Polyphosphates / Q000138:chemical synthesis*; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*
chemical_list: D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; D011122:Polyphosphates; C005692:triphosphoric acid