title: Optimization of ionic liquid based ultrasonic assisted extraction of puerarin from Radix Puerariae Lobatae by response surface methodology.
abstract: Ionic liquid (IL) based ultrasonic assisted extraction (ILUAE) was developed for the effective extraction of puerarin from Radix Puerariae Lobatae (RPL). The ILUAE parameters including the type of ILs, IL concentration, RPL amount, ultrasonic power and time were optimized by single-factor experiment and response surface methodology. Under the optimized experimental conditions, the best results were obtained using RPL amount 0.43 g in 10 mL 1.06 molL(-1) 1-butyl-3-methylimidazolium bromide aqueous solution, ultrasonic time 27.43 min and ultrasonic power 480 W. Scanning electron microscope images of RPL samples were obtained to provide visual evidence of the sonication effect. Compared with the conventional ultrasonic assisted extraction and refluent extraction, the proposed ILUAE offered shorter extraction time and remarkable higher efficiencies due to the higher penetration ability and solubility of IL and the cavitation phenomenon produced in the solvent by the passage of an ultrasonic wave, which further supported the suitability of the proposed approach.
authors.affiliation: Key Laboratory of Poyang Lake Ecology and Bio-Resource Utilization of Ministry of Education, Nanchang University, China. jasperfan@163.com
authors.forename: Jie-Ping
authors.initials: JP
authors.lastname: Fan
authors.forename: Jing
authors.initials: J
authors.lastname: Cao
authors.forename: Xue-Hong
authors.initials: XH
authors.lastname: Zhang
authors.forename: Jun-Zhong
authors.initials: JZ
authors.lastname: Huang
authors.forename: Tao
authors.initials: T
authors.lastname: Kong
authors.forename: Sheng
authors.initials: S
authors.lastname: Tong
authors.forename: Zhe-You
authors.initials: ZY
authors.lastname: Tian
authors.forename: Yan-Long
authors.initials: YL
authors.lastname: Xie
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.forename: Jian-Hang
authors.initials: JH
authors.lastname: Zhu
mesh_terms: D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D052578:Ionic Liquids; D007529:Isoflavones / Q000032:analysis / Q000302:isolation & purification*; D029908:Pueraria / Q000737:chemistry*; D014465:Ultrasonics / Q000379:methods*
chemical_list: D052578:Ionic Liquids; D007529:Isoflavones; C033607:puerarin