title: Route of primary HTLV-1 infection regulates HTLV-1 distribution in reservoir organs of infected mice.
abstract: Human T-cell leukemia virus type-1 (HTLV-1) causes adult T-cell leukemia and HTLV-1-associated myelo-pathy/tropical spastic paraparesis. HTLV-1 is mainly transmitted through blood transfusion and breastfeeding, but viral proliferation in the body in vivo shortly after transmission is not well understood. To investigate whether the route of infection influences the early stages of viral proliferation, we inoculated BALB/c mice with MT-2 cells, an HTLV-1-producing human T-cell line, via different routes, and evaluated the proviral load and humoral immune responses. One month after infection, the provirus was detected in most organs of the mice infected intraperitoneally, and substantial proviral loads were detected in the peripheral blood and secondary lymphoid organs. In contrast, the mice infected intravenously and orally showed low proviral loads, and the provirus distribution was limited to the spinal cord among the intravenously inoculated mice and to the liver among the perorally inoculated mice. Mice infected intraperitoneally also exhibited higher interleukin-2 production than the mice infected intravenously or orally, or than the uninfected control mice, while anti-HTLV-1 antibody titers were comparable between the mice infected intraperitoneally and intravenously. These results demonstrate that the route of primary HTLV-1 infection influences the establishment of HTLV-1-infected cell proliferation and the cell reservoir in mice.
authors.affiliation: Department of Genetics and Cell Biology, Graduate School of Bioscience, Nagahama Institute of Bio-Science and Technology, Nagahama, Shiga 526-0829;
authors.forename: Masakazu
authors.initials: M
authors.lastname: Tanaka
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nitta
authors.forename: Binlian
authors.initials: B
authors.lastname: Sun
authors.forename: Jun-Ichi
authors.initials: JI
authors.lastname: Fujisawa
authors.forename: Masanao
authors.initials: M
authors.lastname: Miwa