title: Iron uptake and homeostasis related genes in potato cultivated in vitro under iron deficiency and overload.
abstract: Potato is one of the most important staple food in the world because it is a good source of vitamin C, vitamin B6 but also an interesting source of minerals including mainly potassium, but also magnesium, phosphorus, manganese, zinc and iron to a lesser extent. The lack of iron constitutes the main form of micronutrient deficiency in the world, namely iron deficiency anemia, which strongly affects pregnant women and children from developing countries. Iron biofortification of major staple food such as potato is thus a crucial issue for populations from these countries. To better understand mechanisms leading to iron accumulation in potato, we followed in an in vitro culture experiment, by qPCR, in the cultivar Désirée, the influence of media iron content on the expression of genes related to iron uptake, transport and homeostasis. As expected, plantlets grown in a low iron medium (1 mg L(-1) FeNaEDTA) displayed a decreased iron content, a strong induction of iron deficiency-related genes and a decreased expression of ferritins. Inversely, plantlets grown in a high iron medium (120 mg L(-1) FeNaEDTA) strongly accumulated iron in roots; however, no significant change in the expression of our set of genes was observed compared to control (40 mg L(-1) FeNaEDTA).
authors.affiliation: Centre de Recherche Public - Gabriel Lippmann, Department EVA, 41, rue du Brill, L-4422 Belvaux, Luxembourg. legay@lippmann.lu
authors.forename: Sylvain
authors.initials: S
authors.lastname: Legay
authors.forename: Cédric
authors.initials: C
authors.lastname: Guignard
authors.forename: Johanna
authors.initials: J
authors.lastname: Ziebel
authors.forename: Danièle
authors.initials: D
authors.lastname: Evers
mesh_terms: D000595:Amino Acid Sequence; D001692:Biological Transport; D018533:Biomass; D016000:Cluster Analysis; D003470:Culture Media; D005293:Ferritins / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis; D007501:Iron / Q000032:analysis / Q000378:metabolism*; D000090463:Iron Deficiencies; D008274:Magnesium / Q000032:analysis; D008903:Minerals / Q000032:analysis; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018520:Plant Shoots / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018547:Plant Stems / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018749:RNA, Plant / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors; D015032:Zinc / Q000032:analysis
chemical_list: D003470:Culture Media; D008903:Minerals; D010940:Plant Proteins; D018749:RNA, Plant; D005293:Ferritins; D007501:Iron; D008274:Magnesium; D015032:Zinc