title: Rapid detection of food pathogens using RNA aptamers-immobilized slide.
abstract: The purpose of this study was to develop a simple and rapid detection system for foodborne bacteria, which consisted of an optical microscope and its slide chip with artificial antibodies, or RNA aptamers. From an RNA pool, three each RNA aptamers were built by the method of SELEX (systematic evolution of ligands by exponential enrichment) for components of cell wall, LPS (lipopolysaccharide) from E. coli O157:H7, teichoic acid from Staphylococcus aureus and a cell membrane protein of OmpC from Salmonella typhimurium, respectively. These aptamers were hybridized with thiol-conjugated 16 dT-linker molecules in order to be immobilized on silver surface which was, in advance, fabricated on glass slide, using a spin-coating method. To confirm that each aptamers retained its specific binding activities to their antigenic live bacteria, microscopic view of bound cells immobilized on silver film were observed. Furthermore, we observed the fluorescence-emitting bacteria-aptamer complex immobilized on silver film after adding RNA aptamers hybridized with fluorophore, FAM-conjugated 16 dT-linker molecules. As a result, the RNA aptamers-immobilized slide system developed in this study was a useful new tool to rapidly monitor individual food pathogens.
authors.affiliation: Bio-Nanotechnology Research Center Korea Food Research Institute, Songnam 463-746, Korea.
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Maeng
authors.forename: Namsoo
authors.initials: N
authors.lastname: Kim
authors.forename: Chong-Tai
authors.initials: CT
authors.lastname: Kim
authors.forename: Seung Ryul
authors.initials: SR
authors.lastname: Han
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Lee
authors.forename: Seong-Wook
authors.initials: SW
authors.lastname: Lee
authors.forename: Myung-Hyun
authors.initials: MH
authors.lastname: Lee
authors.forename: Yong-Jin
authors.initials: YJ
authors.lastname: Cho
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001419:Bacteria / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005504:Food Analysis / Q000295:instrumentation*; D005516:Food Microbiology / Q000295:instrumentation*; D007118:Immunoassay / Q000295:instrumentation*
chemical_list: D052157:Aptamers, Nucleotide