title: Study of protoporphyrin IX elimination by body excreta: a new noninvasive cancer diagnostic method?
abstract: This paper describes the elimination of porphyrins by feces. It was demonstrated that porphyrin accumulates substantially more in tumors than in normal tissues, and consequently more PPIX reaches the blood of patients and animals with tumors, and then, it needs to be eliminated. The fluorescence of feces revealed that there are large amounts of PPIX in the excreta of animals with cancer comparing with healthy animals. The autofluorescence of feces porphyrin extracted with acetone was analyzed using fluorescence spectroscopy of animals inoculated with DU145 cells into the prostate and healthy animals to monitor the PPIX concentration. Emission spectra were obtained by exciting the samples at 405 nm. Significant differences were observed in autofluorescence intensities measured in the 575-725 nm spectral regions for the studied groups. The results showed a noninvasive, simple, rapid and sensitive method to detect cancer by feces analysis.
authors.affiliation: Departamento de Nefrologia, UNIFESP, São Paulo, SP, Brazil. rodrigues.flavia@gmail.com
authors.forename: Flávia R O
authors.initials: FR
authors.lastname: Silva
authors.forename: Camila T
authors.initials: CT
authors.lastname: Nabeshima
authors.forename: Maria H
authors.initials: MH
authors.lastname: Bellini
authors.forename: Nestor
authors.initials: N
authors.lastname: Schor
authors.forename: Nilson D
authors.initials: ND
authors.lastname: Vieira
authors.forename: Lilia C
authors.initials: LC
authors.lastname: Courrol
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D019937:Diagnostic Techniques and Procedures; D005243:Feces / Q000737:chemistry*; D006801:Humans; D008297:Male; D051379:Mice; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D011524:Protoporphyrins / Q000096:biosynthesis / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D011524:Protoporphyrins; C028025:protoporphyrin IX