title: Insect biofuel cells using trehalose included in insect hemolymph leading to an insect-mountable biofuel cell.
abstract: In this paper, an insect biofuel cell (BFC) using trehalose included in insect hemolymph was developed. The insect BFC is based on trehalase and glucose oxidase (GOD) reaction systems which oxidize β-glucose obtained by hydrolyzing trehalose. First, we confirmed by LC-MS that a sufficient amount of trehalose was present in the cockroach hemolymph (CHL). The maximum power density obtained using the insect BFC was 6.07 μW/cm(2). The power output was kept more than 10 % for 2.5 h by protecting the electrodes with a dialysis membrane. Furthermore, the maximum power density was increased to 10.5 μW/cm(2) by using an air diffusion cathode. Finally, we succeeded in driving a melody integrated circuit (IC) and a piezo speaker by connecting five insect BFCs in series. The results indicate that the insect BFC is a promising insect-mountable battery to power environmental monitoring micro-tools.
authors.affiliation: Department of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo, 184-8588, Japan.
authors.forename: Kan
authors.initials: K
authors.lastname: Shoji
authors.forename: Yoshitake
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Masato
authors.initials: M
authors.lastname: Suzuki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hoshino
authors.forename: Nobuhumi
authors.initials: N
authors.lastname: Nakamura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohno
authors.forename: Keisuke
authors.initials: K
authors.lastname: Morishima
mesh_terms: D000818:Animals; D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques / Q000295:instrumentation; D002853:Chromatography, Liquid; D003058:Cockroaches / Q000378:metabolism*; D004058:Diffusion; D004563:Electrochemistry; D004566:Electrodes; D004867:Equipment Design; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism*; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D014198:Trehalase / Q000378:metabolism; D014199:Trehalose / Q000378:metabolism*
chemical_list: D014199:Trehalose; D005949:Glucose Oxidase; D014198:Trehalase; D005947:Glucose