title: An adhesin-like protein, Lam29, from Lactobacillus mucosae ME-340 binds to histone H3 and blood group antigens in human colonic mucus.
abstract: A cell-surface 29-kDa protein (Lam29, cysteine-binding protein of the ABC transporter) from Lactobacillus mucosae ME-340 showed an adhesin-like property for human ABO blood group antigens expressed on the gastrointestinal mucosa. In addition, here we report that Lam29 also bound to an 18-kDa protein on human colonic mucus. By ligand blot assay and N-terminal amino acid sequence of the protein, it was identified as human histone H3. By ligand blot and microplate binding assays with recombinant histone H3, binding between Lam29 and histone H3 was confirmed. The adhesion of ME-340 cells to histone H3 was significantly inhibited by 26% after the addition of 2.5 mg/mL Lam29 as compared to the absence of Lam29 (p<0.01). By GHCl extraction and transcription attenuation of ME-340 cells, binding reduction of ME340 cells against histone H3 was detected at 12% and 13% respectively, as compared to control cells by the BIACORE assay (p<0.01). These data indicate that Lam29 shows multiple binding activities to blood group antigens and histone H3 in human colonic mucus. This is the first report to indicate that lactobacilli expressing Lam29 adhere to histone H3 on gastrointestinal mucosa.
authors.affiliation: Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 981-8555, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Watanabe
authors.forename: Hideki
authors.initials: H
authors.lastname: Kinoshita
authors.forename: I-Nung
authors.initials: IN
authors.lastname: Huang
authors.forename: Kei
authors.initials: K
authors.lastname: Eguchi
authors.forename: Takuya
authors.initials: T
authors.lastname: Tsurumi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kawai
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
authors.forename: Katsunori
authors.initials: K
authors.lastname: Kimura
authors.forename: Naoki
authors.initials: N
authors.lastname: Taketomo
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kikuchi
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Sase
authors.forename: Koh
authors.initials: K
authors.lastname: Miura
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ogawa
authors.forename: Chikashi
authors.initials: C
authors.lastname: Shibata
authors.forename: Akira
authors.initials: A
authors.lastname: Horii
authors.forename: Tadao
authors.initials: T
authors.lastname: Saito
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry / Q000378:metabolism*; D001422:Bacterial Adhesion; D001667:Binding, Competitive; D001706:Biopsy; D001789:Blood Group Antigens / Q000737:chemistry / Q000378:metabolism*; D003106:Colon / Q000378:metabolism* / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000382:microbiology; D007778:Lactobacillus / Q000737:chemistry / Q000378:metabolism*; D009093:Mucus / Q000378:metabolism* / Q000382:microbiology; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D046509:Tissue Culture Techniques
chemical_list: D018829:Adhesins, Bacterial; D001789:Blood Group Antigens; D006657:Histones; D011994:Recombinant Proteins