title: Inhibitory effect of interferon-γ on experimental tooth movement in mice.
abstract: The aim of this study was to investigate the effects of interferon (IFN)-γ on experimental tooth movement in mice using a murine experimental tooth movement model. An Ni-Ti closed-coil spring was inserted between the upper-anterior alveolar bones and the upper-left first molars in mice. We evaluated the relationship between local Ifn-γ mRNA levels and orthodontic tooth movement. In other experiments, IFN-γ was injected adjacent to each first molar every other day during tooth movement. After 12 days, the amount of tooth movement was measured. Tartrate-resistant acid phosphatase (TRAP)-positive cells at the pressure side of each experimental tooth were counted as osteoclasts. Local Ifn-γ mRNA expression increased with orthodontic tooth movement. The number of TRAP-positive cells increased on the pressure side of the first molar. In contrast, the degree of tooth movement and the number of TRAP-positive cells on the pressure side in IFN-γ-injected mice were less than those of control mice. IFN-γ was induced in experimental tooth movement, and could inhibit mechanical force-loaded osteoclastogenesis and tooth movement. These results suggest that IFN-γ might be useful in controlling orthodontic tooth movement because of its inhibitory action on excessive osteoclastogenesis during this movement.
authors.affiliation: Department of Orthodontics and Dentofacial Orthopedics, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Kohara
authors.forename: Hideki
authors.initials: H
authors.lastname: Kitaura
authors.forename: Masako
authors.initials: M
authors.lastname: Yoshimatsu
authors.forename: Yuji
authors.initials: Y
authors.lastname: Fujimura
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Morita
authors.forename: Toshiko
authors.initials: T
authors.lastname: Eguchi
authors.forename: Noriaki
authors.initials: N
authors.lastname: Yoshida
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D008963:Molar; D000071681:Tartrate-Resistant Acid Phosphatase; D013997:Time Factors; D014087:Tooth Movement Techniques
chemical_list: D000998:Antiviral Agents; D007527:Isoenzymes; D007371:Interferon-gamma; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase