title: FITC delivery into plant cells using magnetic single-walled carbon nanotubes.
abstract: In this paper, fluorescein isothiocyanate (FITC) was covalently bonded with magnetic single-walled carbon nanotubes (mSWCNTs) that were purified using our previous method. To demonstrate our design, mSWCNT-FITC was delivered into plant cells (canola and carrot cells) driven by external magnetic forces. From FACS results, the FITC delivery efficiency was about 100% for both two canola and carrot protoplasts, which were further confirmed by the confocal and sectional TEM images. Some mSWCNTs were found trapped both inside the endosomes of canola protoplast and outside endosome near the nuclear membrane of carrot protoplast according to the sectional TEM images. All results showed that mSWCNT is a good delivery carrier for biomolecules.
authors.affiliation: Department of Biomedical Engineering, University of Alberta, Edmonton, Alberta, T6G 2V4, Canada.
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Hao
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yang
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Shi
authors.forename: James
authors.initials: J
authors.lastname: Xing
authors.forename: Janet
authors.initials: J
authors.lastname: Marowitch
authors.forename: Jianmin
authors.initials: J
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Chen