title: Isotype analysis of gerbil-mouse heterohybridomas by RT-PCR.
abstract: We designed primer sets specific to the immunoglobulin (Ig) heavy-chain constant region (IGHC) genes in Mongolian gerbil (Meriones unguiculatus) to amplify five gerbil IGHC cDNA sequences, Cμ, Cγ1, Cγ2, Cε, and Cα. Five gerbil-mouse heterohybridomas B11D2(C2), B11E2(D5).M, B5-3, D5, and C11 respectively expressed Cγ1, Cμ, Cγ2, Cγ2, and Cγ1. In contrast, a commercial isotyping kit for mouse Igs identified Cγ1, Cμ, Cγ3, Cγ3, and Cγ1, respectively, misidentifying gerbil IgG2 as IgG3 by cross-reactivity with anti-mouse IgG3 polyclonal antibody. These primer sets will allow the accurate estimation of gerbil Ig classes and IgG subclasses. These results from three gerbil strains indicate that the primer sets can be used for isotype analysis of gerbil mAbs and for evaluation of humoral immunity.
authors.affiliation: Department of Animal Science and Resources, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa 252-0880, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Ukaji
authors.forename: Osamu
authors.initials: O
authors.lastname: Kai
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000235:genetics*; D005849:Gerbillinae / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007132:Immunoglobulin Isotypes / Q000235:genetics*; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D007127:Immunoglobulin Constant Regions; D007132:Immunoglobulin Isotypes; D004247:DNA