title: The essential polysome-associated RNA-binding protein RBP42 targets mRNAs involved in Trypanosoma brucei energy metabolism.
abstract: RNA-binding proteins that target mRNA coding regions are emerging as regulators of post-transcriptional processes in eukaryotes. Here we describe a newly identified RNA-binding protein, RBP42, which targets the coding region of mRNAs in the insect form of the African trypanosome, Trypanosoma brucei. RBP42 is an essential protein and associates with polysome-bound mRNAs in the cytoplasm. A global survey of RBP42-bound mRNAs was performed by applying HITS-CLIP technology, which captures protein-RNA interactions in vivo using UV light. Specific RBP42-mRNA interactions, as well as mRNA interactions with a known RNA-binding protein, were purified using specific antibodies. Target RNA sequences were identified and quantified using high-throughput RNA sequencing. Analysis revealed that RBP42 bound mainly within the coding region of mRNAs that encode proteins involved in cellular energy metabolism. Although the mechanism of RBP42's function is unclear at present, we speculate that RBP42 plays a critical role in modulating T. brucei energy metabolism.
authors.affiliation: Department of Microbiology and Molecular Genetics, UMDNJ-NJ Medical School, Newark, New Jersey 07103, USA.
authors.forename: Anish
authors.initials: A
authors.lastname: Das
authors.forename: Rachel
authors.initials: R
authors.lastname: Morales
authors.forename: Mahrukh
authors.initials: M
authors.lastname: Banday
authors.forename: Stacey
authors.initials: S
authors.lastname: Garcia
authors.forename: Li
authors.initials: L
authors.lastname: Hao
authors.forename: George A M
authors.initials: GA
authors.lastname: Cross
authors.forename: Antonio M
authors.initials: AM
authors.lastname: Estevez
authors.forename: Vivian
authors.initials: V
authors.lastname: Bellofatto
mesh_terms: D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D001665:Binding Sites; D004734:Energy Metabolism / Q000235:genetics*; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011132:Polyribosomes / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D015800:Protozoan Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism*; D016053:RNA, Protozoan / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; D016601:RNA-Binding Proteins