title: Hypoxia-inducible factor-1α enhances the malignant phenotype of multicellular spheroid HeLa cells in vitro.
abstract: The purpose of this study was to clarify the direct effect of hypoxia-inducible factor-1α (HIF-1α) on tumor growth, apoptosis and migration in vitro. To achieve this aim, a comparison was made of the differences in growth rates, apoptotic indices and cell invasive ability in the human cervical cancer cell line HeLa and the HIF-1α-blocked counterpart in a three-dimensional spheroid culture. A significant decrease in cell proliferation and invasion, and an increase in cell apoptosis were observed in HIF-1α-blocked cells in the three-dimensional culture. The data indicated that a multicellular spheroid culture is an ideal model of hypoxia in vitro and that HIF-1α is a significant regulator of adaptive processes that promote tumor cell malignant phenotypes, such as proliferation, anti-apoptosis and invasive ability.
authors.affiliation: Central Hospital of Wuhan City, Huazhong University of Science and Technology, P.R. China.
authors.forename: Xun
authors.initials: X
authors.lastname: Tian
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
authors.forename: Liangping
authors.initials: L
authors.lastname: Zhao
authors.forename: Junchen
authors.initials: J
authors.lastname: Wei
authors.forename: Hui
authors.initials: H
authors.lastname: Xing
authors.forename: Yuping
authors.initials: Y
authors.lastname: Song
authors.forename: Shixuan
authors.initials: S
authors.lastname: Wang
authors.forename: Ding
authors.initials: D
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Meng
authors.forename: Gang
authors.initials: G
authors.lastname: Chen