title: MicroRNA-467b targets LPL gene in RAW 264.7 macrophages and attenuates lipid accumulation and proinflammatory cytokine secretion.
abstract: LPL (lipoprotein lipase) is a rate-limiting enzyme involved in the hydrolysis of triglycerides. Previous studies have shown that microRNA (miR)-467b regulates hepatic LPL expression and plays a role in the progression of steatosis or abnormal lipid retention in obese mice. Macrophage-derived LPL has been shown to promote atherosclerosis. However, if miR-476b influences macrophage LPL expression and the subsequent effects are unknown. Here, we utilized oxLDL-treatment RAW 264.7 macrophages that were transfected with miR-467b mimics or inhibitors to investigate the potential roles of macrophage miR-476b. We found that miR-467b significantly decreased lipid accumulation and IL-6, IL-1β, TNF-α and MCP-1 secretions. Furthermore, our studies suggested an additional explanation for the regulatory mechanism of miR-467b on its functional target, LPL in RAW 264.7 macrophages. Thus, our findings indicate that miR-467b may regulate lipid accumulation and proinflammatory cytokine secretion in oxLDL-stimulated RAW 264.7 macrophages by targeting the LPL gene.
authors.affiliation: Institute of Cardiovascular Research, Key Laboratory for Atherosclerology of Hunan Province, University of South China, Hengyang 421001, Hunan, China.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Tian
authors.forename: Wu-Jun
authors.initials: WJ
authors.lastname: Chen
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: He
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Tang
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhao
authors.forename: Yun-Cheng
authors.initials: YC
authors.lastname: Lv
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Ouyang
authors.forename: Kai
authors.initials: K
authors.lastname: Yin
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Cheng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.forename: Su-Lan
authors.initials: SL
authors.lastname: Huang
authors.forename: Yuchang
authors.initials: Y
authors.lastname: Fu
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Zhang
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Yin
authors.forename: Chao-Ke
authors.initials: CK
authors.lastname: Tang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D018932:Chemokine CCL2 / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D016207:Cytokines / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000032:analysis*; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D016207:Cytokines; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D015850:Interleukin-6; D008055:Lipids; D008077:Lipoproteins, LDL; C569061:MIRN467 microRNA, mouse; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha; C099252:oxidized low density lipoprotein; D008071:Lipoprotein Lipase