title: Preparation of cationic nanogels for nucleic acid delivery.
abstract: Cationic nanogels with site-selected functionality were designed for the delivery of nucleic acid payloads targeting numerous therapeutic applications. Functional cationic nanogels containing quaternized 2-(dimethylamino)ethyl methacrylate and a cross-linker with reducible disulfide moieties (qNG) were prepared by activators generated by electron transfer (AGET) atom transfer radical polymerization (ATRP) in an inverse miniemulsion. Polyplex formation between the qNG and nucleic acid exemplified by plasmid DNA (pDNA) and short interfering RNA (siRNA duplexes) were evaluated. The delivery of polyplexes was optimized for the delivery of pDNA and siRNA to the  Drosophila  Schneider 2 (S2) cell-line. The qNG/nucleic acid (i.e., siRNA and pDNA) polyplexes were found to be highly effective in their capabilities to deliver their respective payloads.
authors.affiliation: Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.
authors.forename: Saadyah E
authors.initials: SE
authors.lastname: Averick
authors.forename: Eduardo
authors.initials: E
authors.lastname: Paredes
authors.forename: Ainara
authors.initials: A
authors.lastname: Irastorza
authors.forename: Arun R
authors.initials: AR
authors.lastname: Shrivats
authors.forename: Abiraman
authors.initials: A
authors.lastname: Srinivasan
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Siegwart
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Magenau
authors.forename: Hong Y
authors.initials: HY
authors.lastname: Cho
authors.forename: Eric
authors.initials: E
authors.lastname: Hsu
authors.forename: Amram A
authors.initials: AA
authors.lastname: Averick
authors.forename: Jinku
authors.initials: J
authors.lastname: Kim
authors.forename: Shiguang
authors.initials: S
authors.lastname: Liu
authors.forename: Jeffrey O
authors.initials: JO
authors.lastname: Hollinger
authors.forename: Subha R
authors.initials: SR
authors.lastname: Das
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Matyjaszewski
mesh_terms: D000818:Animals; D002412:Cations; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D018014:Gene Transfer Techniques; D008689:Methacrylates / Q000737:chemistry; D000080385:Nanogels; D009696:Nucleic Acids / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection
chemical_list: D002412:Cations; D008689:Methacrylates; D000080385:Nanogels; D009696:Nucleic Acids; D034741:RNA, Small Interfering; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D004247:DNA; C049840:2-(dimethylamino)ethyl methacrylate