title: [Effect of IL-17 on collagen I/III expression in cardiac fibroblasts isolated from BALB/c mice].
abstract: OBJECTIVE
  To investigate the effect of IL-17 on the expression of collagen I/III in cardiac fibroblasts and analyze its molecular mechanism.
  
  
  METHODS
  Cardiac fibroblasts were isolated from 7-14-day-old BALB/c mice and cultured in DMEM with 10% fetal bovine serum (FBS). The cells were collected after IL-17 treatment for 0,  24,  48,  72 h. IL-17 receptors on cardiac fibroblasts were detected by PCR;  the collagen I/III expression was analyzed by immunofluorescence;  the PKCβ,  Erk1/2,  NF-κB phosphorylation were investigated by Western blotting.
  
  
  RESULTS
  IL-17RA/C was expressed on cardiac fibroblasts;  after 24 h of IL-17 stimulation,  the collagen I/III expression obviously increased;  Western blotting showed that PKCβ,  Erk1/2 and NF-κB were phosphorylated on 30,  45,  45 min,  respectively.
  
  
  CONCLUSIONS
  IL-17 could induce the expression of collagen I/III in cardiac fibroblasts,  which might be related with PKCβ-ERK1/2-NF-κB phosphorylation.
authors.affiliation: Institute of Immunology, Jiangsu University, Zhenjiang, China. szl30@yeah.net
authors.forename: Zhao-liang
authors.initials: ZL
authors.lastname: Su
authors.forename: Ying-mei
authors.initials: YM
authors.lastname: Wang
authors.forename: Yan-fang
authors.initials: YF
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.forename: Sha-sha
authors.initials: SS
authors.lastname: Tian
authors.forename: Pan
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiao-yun
authors.initials: XY
authors.lastname: Ji
authors.forename: Ke
authors.initials: K
authors.lastname: Ma
authors.forename: Hua-xi
authors.initials: HX
authors.lastname: Xu
mesh_terms: D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics*; D024061:Collagen Type III / Q000235:genetics*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006321:Heart / Q000187:drug effects*; D020381:Interleukin-17 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D020381:Interleukin-17; D016328:NF-kappa B; D012333:RNA, Messenger; D011493:Protein Kinase C; D048049:Extracellular Signal-Regulated MAP Kinases