title: ABO genotyping by capillary electrophoresis.
abstract: Single-strand conformation polymorphism by capillary electrophoresis (SSCP-CE) has been developed to detect single nucleotide mutations. This method is used to identify the ABO alleles A(1), A(1v), B, O(1), O(1v), and O(2) in this chapter. Four amplicons (112, 121, 123, and 160 bp) labeled with fluorescence are separately amplified by polymerase chain reaction from exons 6 and 7 of ABO gene. These four fragments are combined into a single tube for SSCP-CE analysis using native gel to identify their single nucleotide polymorphism. This method can fast screen ABO genotypes from unknown samples and will be valuable in clinical transfusion or forensic applications.
authors.affiliation: Department of Forensic Medicine, National Taiwan University, Taipei, Taiwan ROC.
authors.forename: James Chun-I
authors.initials: JC
authors.lastname: Lee
authors.forename: Hsing-Mei
authors.initials: HM
authors.lastname: Hsieh
authors.forename: Hsiao-Feng
authors.initials: HF
authors.lastname: Teng
authors.forename: Li-Chin
authors.initials: LC
authors.lastname: Tsai
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D019075:Electrophoresis, Capillary / Q000379:methods*; D005838:Genotype; D060005:Genotyping Techniques / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D000017:ABO Blood-Group System