title: RHBDD2: a 5-fluorouracil responsive gene overexpressed in the advanced stages of colorectal cancer.
abstract: In previous studies, we identified rhomboid domain containing 2 (RHBDD2) gene to be markedly overexpressed in breast cancer patients that developed recurrence of the disease. In this study, we evaluated for the first time RHBDD2 gene expression in colorectal cancer (CRC). Five public available DNA microarray studies were compiled in a homogeneous dataset of 906 colorectal samples. The statistical analysis of these data showed a significant increase of RHBDD2 expression in the advanced stages of CRC (p < 0.01). We validated these findings by immunohistochemistry on 130 colorectal tissue samples; RHBDD2 protein overexpression was also observed in the advanced stages of the disease (p < 0.001). In addition, we investigated RHBDD2 expression in response to the chemotherapy agent 5-fluorouracile (5FU). We detected a significant increase of RHBDD2 mRNA and protein after 5FU treatment (20-40 μM; p < 0.001). Overall, these results showed that RHBDD2 overexpression might play a role in colorectal cancer progression.
authors.affiliation: CINIBA, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina. ezequiellacunza@hotmail.com
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Lacunza
authors.forename: R
authors.initials: R
authors.lastname: Canzoneri
authors.forename: M E
authors.initials: ME
authors.lastname: Rabassa
authors.forename: A
authors.initials: A
authors.lastname: Zwenger
authors.forename: A
authors.initials: A
authors.lastname: Segal-Eiras
authors.forename: M V
authors.initials: MV
authors.lastname: Croce
authors.forename: M C
authors.initials: MC
authors.lastname: Abba
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000627:therapeutic use*; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D016022:Case-Control Studies; D003106:Colon / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D005472:Fluorouracil / Q000627:therapeutic use*; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D008565:Membrane Proteins; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012007:Rectum / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D000964:Antimetabolites, Antineoplastic; D014408:Biomarkers, Tumor; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C544929:RHBDD2 protein, human; D012333:RNA, Messenger; D005472:Fluorouracil