title: EZ Gluten for the qualitative detection of gluten in foods, beverages, and environmental surfaces.
abstract: The EZ Gluten assay is a rapid immunochromatographic screening method for qualitative detection of gluten in raw and cooked foods and beverages and on environmental surfaces. This AOAC Performance Tested Method study evaluated the EZ Gluten assay as an effective method for the detection of gluten in four selected matrixes: rice flour, cooked dough, beer, and dog food. In addition, the method was evaluated for its effectiveness in detecting gluten contamination of > or =1 microg/2 in.2 (25 cm2) stainless steel surface area. The EZ Gluten demonstrated 100% specificity [probability of detection (POD) 0.00, confidence interval (CI) 0.00-0.01] and 99% sensitivity (POD 0.99, CI 0.97-0.995) at the 10 ppm level for all four matrixes, and 100% specificity (POD 0.00, CI 0.00-0.11) and sensitivity (POD 1.00, CI 0.886-1.00) at the 1 microg level on the stainless steel surface. Independent laboratory testing confirmed the internal validation results in one matrix and on the stainless steel surface. Lot-to-lot, stability, and robustness studies provided evidence that the EZ Gluten is a rugged, consistent method for the detection of gluten at levels as low as 10 ppm.
authors.affiliation: ELISA Technologies, Inc., 2501 NW 66th Ct, Gainesville, FL 32653, USA. laura.allred@elisa-tek.com
authors.forename: Laura K
authors.initials: LK
authors.lastname: Allred
authors.forename: Eun S
authors.initials: ES
authors.lastname: Park
mesh_terms: D052179:Antigens, Plant / Q000032:analysis*; D001628:Beverages / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005502:Food; D005504:Food Analysis / Q000379:methods*; D005983:Glutens / Q000032:analysis* / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007753:Laboratories; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013193:Stainless Steel
chemical_list: D052179:Antigens, Plant; D011933:Reagent Kits, Diagnostic; D013193:Stainless Steel; D005983:Glutens