title: A novel Fe(II)/α-ketoglutarate-dependent dioxygenase from Burkholderia ambifaria has β-hydroxylating activity of N-succinyl l-leucine.
abstract: An Fe(II)/α-ketoglutarate-dependent dioxygenase, SadA, was obtained from Burkholderia ambifaria AMMD and heterologously expressed in Escherichia coli. Purified recombinant SadA had catalytic activity towards several N-substituted l-amino acids, which was especially strong with N-succinyl l-leucine. With the NMR and LC-MS analysis, SadA converted N-succinyl l-leucine into N-succinyl l-threo-β-hydroxyleucine with >99% diastereoselectivity. SadA is the first enzyme catalysing β-hydroxylation of aliphatic amino acid-related substances and a potent biocatalyst for the preparation of optically active β-hydroxy amino acids.
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hibi
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kawashima
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kasahara
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: P M
authors.initials: PM
authors.lastname: Sokolov
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: S V
authors.initials: SV
authors.lastname: Smirnov
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kodera
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sugiyama
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shimizu
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yokozeki
authors.affiliation: Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan Ajinomoto-Genetika Research Institute, Moscow, Russia Institute of Food Sciences & Technologies Food Product Division, Ajinomoto Co., Inc., Kawasaki, Japan Research Institute for Bioscience Products & Fine Chemicals, Ajinomoto Co., Inc., Kawasaki, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Ogawa
mesh_terms: D019117:Burkholderia / Q000201:enzymology* / Q000235:genetics; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006900:Hydroxylation / Q000235:genetics; D007656:Ketoglutaric Acids / Q000737:chemistry / Q000378:metabolism; D007930:Leucine / Q000031:analogs & derivatives* / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D013386:Succinates / Q000737:chemistry / Q000378:metabolism*
chemical_list: D007656:Ketoglutaric Acids; D013386:Succinates; D049308:Dioxygenases; D007930:Leucine