title: 3D multi-isotope imaging mass spectrometry reveals penetration of 18O-trehalose in mouse sperm nucleus.
abstract: The prevalence of genetically engineered mice in medical research has led to ever increasing storage costs. Trehalose has a significant beneficial effect in preserving the developmental potential of mouse sperm following partial desiccation and storage at temperatures above freezing. Using multi-isotope imaging mass spectrometry, we are able to image and measure trehalose in individual spermatozoa. We provide the first evidence that trehalose penetrates the nucleus of a mammalian cell, permitting tolerance to desiccation. These results have broad implications for long-term storage of mammalian cells.
authors.affiliation: National Resource for Imaging Mass Spectrometry, Division of Genetics, Harvard Medical School, Brigham and Women's Hospital, Cambridge, Massachusetts, United States of America. cpl@harvard.edu
authors.forename: Claude P
authors.initials: CP
authors.lastname: Lechene
authors.forename: Gloria Y
authors.initials: GY
authors.lastname: Lee
authors.forename: J Collin
authors.initials: JC
authors.lastname: Poczatek
authors.forename: Mehmet
authors.initials: M
authors.lastname: Toner
authors.forename: John D
authors.initials: JD
authors.lastname: Biggers
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000000981:diagnostic imaging* / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D003890:Desiccation / Q000379:methods; D005818:Genetic Engineering / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D010103:Oxygen Isotopes / Q000494:pharmacology*; D011877:Radionuclide Imaging; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000000981:diagnostic imaging* / Q000378:metabolism; D013696:Temperature; D014199:Trehalose / Q000737:chemistry*
chemical_list: D002843:Chromatin; D010103:Oxygen Isotopes; D014199:Trehalose