title: Subnormothermic machine perfusion for non-heart-beating donor liver grafts preservation in a Swine model: a new strategy to increase the donor pool?
abstract: We previously reported that subnormothermic machine perfusion (sMP; 20°C) is able to improve the preservation of livers obtained from non-heart-beating donors (NHBDs) in rats. We have compared sMP and standard cold storage (CS) to preserve pig livers after 60 minutes of cardiac arrest. In the sMP group livers were perfused for 6 hours with Celsior at 20°C. In the CS group they were stored in Celsior at 4°C for 6 hours as usual. To simulate liver transplantation, both sMP- and CS-preserved livers were reperfused using a mechanical continuous perfusion system with autologus blood for 2 hours at 37°C. At 120 min after reperfusion aspartate aminotransferase levels in sMP versus CS were 499 ± 198 versus 7648 ± 2806 U/L (P < .01); lactate dehydrogenase 1685 ± 418 versus 12998 ± 3039 U/L (P < .01); and lactic acid 4.78 ± 3.02 versus 10.46 ± 1.79 mmol/L (P < .01) respectively. The sMP group showed better histopathologic results with significantly less hepatic damage. This study confirmed that sMP was able to resuscitate liver grafts from large NHBD animals.
authors.affiliation: Department of General Surgery and Organ Transplantation, Hepatobiliary Surgery and Liver Transplantation Unit, University of Padova, Padova, Italy. enrico.gringeri@unipd.it
authors.forename: E
authors.initials: E
authors.lastname: Gringeri
authors.forename: P
authors.initials: P
authors.lastname: Bonsignore
authors.forename: D
authors.initials: D
authors.lastname: Bassi
authors.forename: F E
authors.initials: FE
authors.lastname: D'Amico
authors.forename: C
authors.initials: C
authors.lastname: Mescoli
authors.forename: M
authors.initials: M
authors.lastname: Polacco
authors.forename: M
authors.initials: M
authors.lastname: Buggio
authors.forename: R
authors.initials: R
authors.lastname: Luisetto
authors.forename: R
authors.initials: R
authors.lastname: Boetto
authors.forename: G
authors.initials: G
authors.lastname: Noaro
authors.forename: A
authors.initials: A
authors.lastname: Ferrigno
authors.forename: E
authors.initials: E
authors.lastname: Boncompagni
authors.forename: I
authors.initials: I
authors.lastname: Freitas
authors.forename: M P
authors.initials: MP
authors.lastname: Vairetti
authors.forename: A
authors.initials: A
authors.lastname: Carraro
authors.forename: D
authors.initials: D
authors.lastname: Neri
authors.forename: U
authors.initials: U
authors.lastname: Cillo
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001831:Body Temperature; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D005978:Glutathione; D006639:Histidine; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016031:Liver Transplantation; D008353:Mannitol; D023421:Models, Animal; D009200:Myocardial Contraction; D019188:Organ Preservation Solutions; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D013552:Swine; D014019:Tissue Donors
chemical_list: C088453:Celsior; D004187:Disaccharides; D004573:Electrolytes; D005971:Glutamates; D019188:Organ Preservation Solutions; D008353:Mannitol; D006639:Histidine; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D005978:Glutathione