title: Leptospiral outer membrane protein microarray, a novel approach to identification of host ligand-binding proteins.
abstract: Leptospirosis is a zoonosis with worldwide distribution caused by pathogenic spirochetes belonging to the genus Leptospira. The leptospiral life cycle involves transmission via freshwater and colonization of the renal tubules of their reservoir hosts. Infection requires adherence to cell surfaces and extracellular matrix components of host tissues. These host-pathogen interactions involve outer membrane proteins (OMPs) expressed on the bacterial surface. In this study, we developed an Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 OMP microarray containing all predicted lipoproteins and transmembrane OMPs. A total of 401 leptospiral genes or their fragments were transcribed and translated in vitro and printed on nitrocellulose-coated glass slides. We investigated the potential of this protein microarray to screen for interactions between leptospiral OMPs and fibronectin (Fn). This approach resulted in the identification of the recently described fibronectin-binding protein, LIC10258 (MFn8, Lsa66), and 14 novel Fn-binding proteins, denoted Microarray Fn-binding proteins (MFns). We confirmed Fn binding of purified recombinant LIC11612 (MFn1), LIC10714 (MFn2), LIC11051 (MFn6), LIC11436 (MFn7), LIC10258 (MFn8, Lsa66), and LIC10537 (MFn9) by far-Western blot assays. Moreover, we obtained specific antibodies to MFn1, MFn7, MFn8 (Lsa66), and MFn9 and demonstrated that MFn1, MFn7, and MFn9 are expressed and surface exposed under in vitro growth conditions. Further, we demonstrated that MFn1, MFn4 (LIC12631, Sph2), and MFn7 enable leptospires to bind fibronectin when expressed in the saprophyte, Leptospira biflexa. Protein microarrays are valuable tools for high-throughput identification of novel host ligand-binding proteins that have the potential to play key roles in the virulence mechanisms of pathogens.
authors.affiliation: Research Service, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California, USA. mpinne@ucla.edu
authors.forename: Marija
authors.initials: M
authors.lastname: Pinne
authors.forename: James
authors.initials: J
authors.lastname: Matsunaga
authors.forename: David A
authors.initials: DA
authors.lastname: Haake
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D007919:Leptospira / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D015203:Reproducibility of Results; D059467:Transcriptome
chemical_list: D001425:Bacterial Outer Membrane Proteins; D005353:Fibronectins; D008024:Ligands