title: Antimutagenic and antioxidant activity of Lisosan G in Saccharomyces cerevisiae.
abstract: In the present study the antimutagenic and antioxidant effects of a powder of grain (Lisosan G) in yeast Saccharomyces cerevisiae were studied. Results showed that Lisosan G treatment decreased significantly the intracellular ROS concentration and mutagenesis induced by hydrogen peroxide in S. cerevisiae D7 strain. The effect of Lisosan G was then evaluated by using superoxide dismutase (SOD) proficient and deficient strains of S. cerevisiae. Lisosan G showed protective activity in sod1Δ and sod2Δ mutant strains, indicating an in vivo antioxidant effect. A high radical scavenging activity of Lisosan G was also demonstrated in vitro using the oxygen radical absorbance capacity (ORAC) assay. The obtained results showed a protective effect of Lisosan G in yeast cells, indicating that its antioxidant capacity contributes to its antimutagenic action.
authors.affiliation: National Research Council, Institute of Biology and Agricultural Biotechnology (IBBA), Pisa Unit, Research Area of Pisa, Via Moruzzi 1, 56124 Pisa, Italy. frassinetti@ibba.cnr.it
authors.forename: Stefania
authors.initials: S
authors.lastname: Frassinetti
authors.forename: Clara Maria
authors.initials: CM
authors.lastname: Della Croce
authors.forename: Leonardo
authors.initials: L
authors.lastname: Caltavuturo
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Longo
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity; D009154:Mutation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D028321:Plant Preparations; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism
chemical_list: D016587:Antimutagenic Agents; D000975:Antioxidants; C522933:Lisosan G; D009153:Mutagens; D010936:Plant Extracts; D028321:Plant Preparations; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide