title: Experimental study of the effect of autologous platelet-rich plasma on the early phases of osteoinduction by allogenic demineralized bone matrix.
abstract: OBJECTIVE
  To evaluate the effect of autologous platelet-rich plasma (PRP) on the early phases of osteoinduction by allogenic demineralized bone matrix (DBM) in rabbit intramuscular positions.
  
  
  METHODS
  Allogenic DBM was produced from bones of 3 healthy rabbits. In each of 6 experimental animals, 0.3 mL autologous PRP was prepared and 2 muscle pouches were created, where 250 mg DBM + PRP (experimental sites) and 250 mg DBM without PRP (control sites) were randomly implanted. Animals were euthanized 3 weeks postoperatively.
  
  
  RESULTS
  Histologic examination revealed uneventful healing in all cases, whereas remineralization of the periphery of the bone graft particles was a constant finding. In both control and experimental sites, fibroblasts and other mesenchymal cells (probably osteoprogenitor cells and preosteoblasts) were observed. The main histological difference was the recolonization of the empty lacunae of the bone graft particles by osteocytes at the control sites. The degradation of the graft at the control sites was statistically significantly quicker, although a statistically significant difference regarding the amount of the newly formed fibrous connective tissue was not observed.
  
  
  CONCLUSIONS
  The present study demonstrated that in this experimental model, the addition of PRP to DBM had a negative effect on the early phases of osteoinduction at 3 weeks of observation.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Dental School, University of Athens, Athens, Greece. dr.minasleventis@gmail.com
authors.forename: Minas D
authors.initials: MD
authors.lastname: Leventis
authors.forename: Efstathios
authors.initials: E
authors.lastname: Eleftheriadis
authors.forename: Panagiota
authors.initials: P
authors.lastname: Oikonomopoulou
authors.forename: Helen
authors.initials: H
authors.lastname: Vavouraki
authors.forename: Lubna
authors.initials: L
authors.lastname: Khaldi
authors.forename: Konstantinos I
authors.initials: KI
authors.lastname: Tosios
authors.forename: Emmanouil
authors.initials: E
authors.lastname: Vardas
authors.forename: Konstantinos D
authors.initials: KD
authors.lastname: Valavanis
authors.forename: Ismene
authors.initials: I
authors.lastname: Dontas
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000637:transplantation; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000009:adverse effects*; D006084:Graft Rejection; D008297:Male; D053657:Platelet-Rich Plasma; D011817:Rabbits; D011897:Random Allocation
chemical_list: D018786:Bone Substitutes