title: [Analyses on antigen epitopes and drug resistance mutations of HIV-1 gag and pol genes].
abstract: To study the CTL antigen epitopes and drug resistance mutations of HIV-1 gag and pol genes through analyzing gag and pol gene sequences. The HIV-1 gag and pol gene fragments were amplified using nested polymerase chain reaction. A total of 23 PCR sequences, 449 cloned gag sequences and 402 cloned pol sequences were obtained. Sequence analyses showed the 23 samples were subtype B or B'. A total of 4 in 8 CTL antigen epitopes appeared 8 mutations in consensus sequence of subtype B and B'. There were no mutations found in the PCR sequences, whereas a few mutations were found in clone sequences (9.80%) in 5 antigen epitopes in p24 region. Eighteen PIs-related mutations and 24 RTIs-related mutations were found in PCR sequences and clone sequences in pol gene region, in which 17 (94.44%) PIs-related mutations and 15 (62.50%) RTIs-related mutations were found only in the clone sequences, respectively. The results showed that the prevalence of HIV-1 drug resistance strains in this study was at a higher level (17.39%), suggesting that some samples were resistant.to existing antiviral drugs.
authors.affiliation: State Key Laboratory for Infections Disease Prevention and Control, Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 100052, China. cz_shang@163.com
authors.forename: Cheng-Zhang
authors.initials: CZ
authors.lastname: Shang
authors.forename: Guo-Min
authors.initials: GM
authors.lastname: Chen
authors.forename: Huai-Yu
authors.initials: HY
authors.lastname: Zhang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000956:Antigens, Viral / Q000276:immunology*; D004252:DNA Mutational Analysis; D024882:Drug Resistance, Viral / Q000235:genetics*; D000939:Epitopes / Q000276:immunology*; D015497:HIV-1 / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000276:immunology*; D054298:Human Immunodeficiency Virus Proteins / Q000235:genetics*; D009154:Mutation; D010802:Phylogeny; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D054298:Human Immunodeficiency Virus Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus