title: Optimization and application of subtype specific polymerase chain reaction for detection and identification of mixed subtypes of hepatitis C virus in patients with renal disease.
abstract: The main objective of the study is to optimize a subtype specific polymerase chain reaction (PCR) for identification of hepatitis C virus (HCV) subtypes in patients with renal disease. Thirty two peripheral blood specimens obtained from 28 patients (post renal transplant N=14, chronic kidney disease N=14) were subjected to HCV viral load determination followed by genotyping analysis. Based on the mixed genotypes and subtypes (>one subtype) obtained by type specific PCR, specific patterns from Pattern I through Pattern X were assigned. All the 32 peripheral blood specimens revealed mixed HCV subtype patterns (>one subtype). The detection of Pattern I in 12 (42.8%) out of 28 patients was statistically significant (Chi square test, P value<0.001). HCV subtyping assay developed using stringent thermal profile revealed the presence of mixed subtype patterns (>one subtype) which is for the first time being reported in literature.
authors.affiliation: Larsen and Toubro Microbiology Research Centre, Kamal Nayan Bajaj Research Centre, Vision Research Foundation, No. 41, College Road, Chennai 600006, India.
authors.forename: Prathiba
authors.initials: P
authors.lastname: K
authors.forename: Bagyalakshmi
authors.initials: B
authors.lastname: R
authors.forename: Malathi
authors.initials: M
authors.lastname: J
authors.forename: Anusha
authors.initials: A
authors.lastname: H
authors.forename: Ravichandran
authors.initials: R
authors.lastname: R
authors.forename: Chandy
authors.initials: C
authors.lastname: G
authors.forename: Madhavan
authors.initials: M
authors.lastname: H N
mesh_terms: D060085:Coinfection / Q000821:virology; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000150:complications* / Q000821:virology*; D006801:Humans; D007674:Kidney Diseases / Q000150:complications*; D016030:Kidney Transplantation / Q000009:adverse effects; D016133:Polymerase Chain Reaction / Q000379:methods*; D019562:Viral Load; D014773:Virology / Q000379:methods*