title: Ionic liquids induced structural changes of bovine serum albumin in aqueous media: a detailed physicochemical and spectroscopic study.
abstract: Structural changes of a globular protein, bovine serum albumin (BSA), as a consequence of interaction with the surface active ionic liquids (ILs)-3-methyl-1-octylimidazolium chloride, [C(8)mim][Cl], and 1-butyl-3-methylimidazolium octylsulfate, [C(4)mim][C(8)OSO(3)]-have been investigated using various physicochemical and spectroscopic techniques such as tensiometry, conductometry, steady-state fluorescence, far-UV circular dichroism spectroscopy (CD), and dynamic light scattering (DLS). The interactional behavior of ILs (monomers and self-assembled structures) toward BSA in different IL concentration regimes at the air/solution interface as well as in the bulk is investigated and discussed depending upon the nature of ions of ILs. CD combined with the steady state fluorescence spectroscopy provided valuable insights into the unfolding of BSA as a consequence of IL binding. The complementary results obtained from the multitechnique approach proved very useful in drawing out the mechanism of interaction between ILs and BSA in different IL concentration regimes.
authors.affiliation: Department of Chemistry and Biochemistry, Graduate School of Engineering, Centre for Molecular Systems (CMS), Kyushu University, 744-Moto-oka, Fukuoka-819-0395, Japan.
authors.forename: Tejwant
authors.initials: T
authors.lastname: Singh
authors.forename: Pankaj
authors.initials: P
authors.lastname: Bharmoria
authors.forename: Masa-aki
authors.initials: MA
authors.lastname: Morikawa
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kimizuka
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000818:Animals; D002021:Buffers; D002417:Cattle; D055598:Chemical Phenomena; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D052578:Ionic Liquids / Q000494:pharmacology*; D008823:Micelles; D008958:Models, Molecular; D011487:Protein Conformation / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013057:Spectrum Analysis; D014867:Water / Q000737:chemistry*
chemical_list: D002021:Buffers; D052578:Ionic Liquids; D008823:Micelles; D014867:Water; D012710:Serum Albumin, Bovine