title: Innate non-specific cell substratum adhesion.
abstract: Adhesion of motile cells to solid surfaces is necessary to transmit forces required for propulsion. Unlike mammalian cells, Dictyostelium cells do not make integrin mediated focal adhesions. Nevertheless, they can move rapidly on both hydrophobic and hydrophilic surfaces. We have found that adhesion to such surfaces can be inhibited by addition of sugars or amino acids to the buffer. Treating whole cells with αlpha-mannosidase to cleave surface oligosaccharides also reduces adhesion. The results indicate that adhesion of these cells is mediated by van der Waals attraction of their surface glycoproteins to the underlying substratum. Since glycoproteins are prevalent components of the surface of most cells, innate adhesion may be a common cellular property that has been overlooked.
authors.affiliation: Section of Cell and Developmental Biology, Division of Biological Sciences, University of California San Diego, La Jolla, California, United States of America. wloomis@ucsd.edu
authors.forename: William F
authors.initials: WF
authors.lastname: Loomis
authors.forename: Danny
authors.initials: D
authors.lastname: Fuller
authors.forename: Edgar
authors.initials: E
authors.lastname: Gutierrez
authors.forename: Alex
authors.initials: A
authors.lastname: Groisman
authors.forename: Wouter-Jan
authors.initials: WJ
authors.lastname: Rappel
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002465:Cell Movement; D003470:Culture Media; D004023:Dictyostelium / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D016023:Integrins / Q000378:metabolism; D007700:Kinetics; D044085:Microfluidics; D009844:Oligosaccharides / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000596:Amino Acids; D003470:Culture Media; D006023:Glycoproteins; D016023:Integrins; D009844:Oligosaccharides; D005947:Glucose