title: Tubular waveguide evanescent field absorption biosensor based on particle plasmon resonance for multiplex label-free detection.
abstract: A novel tubular waveguide particle plasmon resonance (TW-PPR) sensor is demonstrated for label-free biochemical detection. The sensor itself is a microchamber of a defined sample volume, a mechanical support for sensor coating, a waveguide to provide evanescent wave interrogation, and it can be easily extended to a multi-channel format. The sensor resolution is estimated to be 2.6×10(-6) RIU in measuring solutions of various refractive indices. The sensing system can perform multiple measurements simultaneously and its limit of detection for anti-DNP antibody and streptavidin is 1.2×10(-10) g/ml (0.55 pM) and 2.3×10(-10) g/ml (3.5 pM), respectively. Accurate determination of these analytes with known concentration spiked in artificial urine were examined and the bias is less than ±7%, supporting the utility of the device for analyte screening in more complex media. The TW-PPR sensor can be inexpensively fabricated and has a special niche for monitoring biomolecular interactions in real-time, hence it is ideally suitable for disposable uses, especially promising for convenient high-throughput biochemical sensing applications.
authors.affiliation: Center for Nano Bio-Detection, National Chung Cheng University, 168 University Road, Min-Hsiung, Chiayi 621, Taiwan.
authors.forename: Hsing-Ying
authors.initials: HY
authors.lastname: Lin
authors.forename: Chen-Han
authors.initials: CH
authors.lastname: Huang
authors.forename: Shin-Huei
authors.initials: SH
authors.lastname: Chen
authors.forename: Yu-Chia
authors.initials: YC
authors.lastname: Liu
authors.forename: Wei-Zhe
authors.initials: WZ
authors.lastname: Chang
authors.forename: Lai-Kwan
authors.initials: LK
authors.lastname: Chau
mesh_terms: D000042:Absorption; D001681:Biological Assay / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007118:Immunoassay / Q000295:instrumentation*; D008027:Light; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D020349:Surface Plasmon Resonance / Q000295:instrumentation*