title: A strategy to enhance the binding affinity of fluorophore-aptamer pairs for RNA tagging with neomycin conjugation.
abstract: Fluorogenic sulforhodamine-neomycin conjugates have been designed and synthesized for RNA tagging. Conjugates were fluorescently activated by binding to RNA aptamers and exhibited greater than 250-400 fold enhancement in binding affinity relative to corresponding unconjugated fluorophores.
authors.affiliation: Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Stanford, California 94305-5484, USA.
authors.forename: Jongho
authors.initials: J
authors.lastname: Jeon
authors.forename: Kyung Hyun
authors.initials: KH
authors.lastname: Lee
authors.forename: Jianghong
authors.initials: J
authors.lastname: Rao
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001665:Binding Sites; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D009355:Neomycin / Q000138:chemical synthesis / Q000737:chemistry*; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D012235:Rhodamines; D009355:Neomycin