title: Drosophila melanogaster Acetyl-CoA-carboxylase sustains a fatty acid-dependent remote signal to waterproof the respiratory system.
abstract: Fatty acid (FA) metabolism plays a central role in body homeostasis and related diseases. Thus, FA metabolic enzymes are attractive targets for drug therapy. Mouse studies on Acetyl-coenzymeA-carboxylase (ACC), the rate-limiting enzyme for FA synthesis, have highlighted its homeostatic role in liver and adipose tissue. We took advantage of the powerful genetics of Drosophila melanogaster to investigate the role of the unique Drosophila ACC homologue in the fat body and the oenocytes. The fat body accomplishes hepatic and storage functions, whereas the oenocytes are proposed to produce the cuticular lipids and to contribute to the hepatic function. RNA-interfering disruption of ACC in the fat body does not affect viability but does result in a dramatic reduction in triglyceride storage and a concurrent increase in glycogen accumulation. These metabolic perturbations further highlight the role of triglyceride and glycogen storage in controlling circulatory sugar levels, thereby validating Drosophila as a relevant model to explore the tissue-specific function of FA metabolic enzymes. In contrast, ACC disruption in the oenocytes through RNA-interference or tissue-targeted mutation induces lethality, as does oenocyte ablation. Surprisingly, this lethality is associated with a failure in the watertightness of the spiracles-the organs controlling the entry of air into the trachea. At the cellular level, we have observed that, in defective spiracles, lipids fail to transfer from the spiracular gland to the point of air entry. This phenotype is caused by disrupted synthesis of a putative very-long-chain-FA (VLCFA) within the oenocytes, which ultimately results in a lethal anoxic issue. Preventing liquid entry into respiratory systems is a universal issue for air-breathing animals. Here, we have shown that, in Drosophila, this process is controlled by a putative VLCFA produced within the oenocytes.
authors.affiliation: CNRS, Centre de Génétique Moléculaire, UPR 3404, Gif-sur-Yvette, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Parvy
authors.forename: Laura
authors.initials: L
authors.lastname: Napal
authors.forename: Thomas
authors.initials: T
authors.lastname: Rubin
authors.forename: Mickael
authors.initials: M
authors.lastname: Poidevin
authors.forename: Laurent
authors.initials: L
authors.lastname: Perrin
authors.forename: Claude
authors.initials: C
authors.lastname: Wicker-Thomas
authors.forename: Jacques
authors.initials: J
authors.lastname: Montagne
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D005216:Fat Body / Q000166:cytology / Q000378:metabolism; D005227:Fatty Acids / Q000235:genetics / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics*; D034622:RNA Interference; D012137:Respiratory System / Q000378:metabolism*; D014280:Triglycerides / Q000235:genetics / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D014280:Triglycerides; D014867:Water; D006003:Glycogen; D000103:Acetyl-CoA Carboxylase