title: miRNA-218 contributes to the regulation of D-glucuronyl C5-epimerase expression in normal and tumor breast tissues.
abstract: microRNAs (miRNAs) are key posttranscriptional regulators of gene expression. In the present study, regulation of tumor-suppressor gene D-glucuronyl C5-epimerase (GLCE) by miRNA-218 was investigated. Significant downregulation of miRNA-218 expression was shown in primary breast tumors. Exogenous miRNA-218/anti-miRNA-218 did not affect GLCE mRNA but regulated GLCE protein level in MCF7 breast carcinoma cells in vitro. Comparative analysis showed a positive correlation between miRNA-218 and GLCE mRNA, and negative correlation between miRNA-218 and GLCE protein levels in breast tissues and primary tumors in vivo, supporting a direct involvement of miRNA-218 in posttranscriptional regulation of GLCE in human breast tissue. A common scheme for the regulation of GLCE expression in normal and tumor breast tissues is suggested.
authors.affiliation: Institute of Molecular Biology and Biophysics, Siberian Department of Russian Academy of Medical Sciences, Novosibirsk, Russia.
authors.forename: Tatiana Y
authors.initials: TY
authors.lastname: Prudnikova
authors.forename: Luydmila A
authors.initials: LA
authors.lastname: Mostovich
authors.forename: Vladimir I
authors.initials: VI
authors.lastname: Kashuba
authors.forename: Ingemar
authors.initials: I
authors.lastname: Ernberg
authors.forename: Eugene R
authors.initials: ER
authors.lastname: Zabarovsky
authors.forename: Elvira V
authors.initials: EV
authors.lastname: Grigorieva
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D002238:Carbohydrate Epimerases / Q000235:genetics / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D002238:Carbohydrate Epimerases; C019591:heparosan N-sulfate D-glucuronosyl 5-epimerase