title: Identification and characterization of a novel antimicrobial peptide from the venom of the ant Tetramorium bicarinatum.
abstract: A novel antimicrobial peptide, named Bicarinalin, has been isolated from the venom of the ant Tetramorium bicarinatum. Its amino acid sequence has been determined by de novo sequencing using mass spectrometry and by Edman degradation. Bicarinalin contained 20 amino acid residues and was C-terminally amidated as the majority of antimicrobial peptides isolated to date from insect venoms. Interestingly, this peptide had a linear structure and exhibited no meaningful similarity with any known peptides. Antibacterial activities against Staphylococcus aureus and S. xylosus strains were evaluated using a synthetic replicate. Bicarinalin had a potent and broad antibacterial activity of the same magnitude as Melittin and other hymenopteran antimicrobial peptides such as Pilosulin or Defensin. Moreover, this antimicrobial peptide has a weak hemolytic activity compared to Melittin on erythrocytes, suggesting potential for development into an anti-infective agent for use against emerging antibiotic-resistant pathogens.
authors.affiliation: Equipe VacBio EA 4357, PRES Université Toulouse, CUFR JF Champollion, Place de Verdun, 81012 Albi, France.
authors.forename: Aline
authors.initials: A
authors.lastname: Rifflet
authors.forename: Sabine
authors.initials: S
authors.lastname: Gavalda
authors.forename: Nathan
authors.initials: N
authors.lastname: Téné
authors.forename: Jérôme
authors.initials: J
authors.lastname: Orivel
authors.forename: Jérôme
authors.initials: J
authors.lastname: Leprince
authors.forename: Laure
authors.initials: L
authors.lastname: Guilhaudis
authors.forename: Eric
authors.initials: E
authors.lastname: Génin
authors.forename: Angélique
authors.initials: A
authors.lastname: Vétillard
authors.forename: Michel
authors.initials: M
authors.lastname: Treilhou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000862:Ant Venoms / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001000:Ants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D021241:Spectrometry, Mass, Electrospray Ionization; D013210:Staphylococcus / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects*; D013329:Structure-Activity Relationship; D053719:Tandem Mass Spectrometry; D014688:Venoms / Q000737:chemistry*
chemical_list: D000862:Ant Venoms; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D014688:Venoms; C581022:bicarinalin, Tetramorium bicarinatum