title: Development and characterization of EST-SSR markers from Scapharca broughtonii and their transferability in Scapharca subcrenata and Tegillarca granosa.
abstract: Twenty-five novel EST-derived simple sequence repeat (EST-SSR) markers were developed in the ark shell Scapharca broughtonii. Polymorphisms of these EST-SSR markers were evaluated in 48 wild individuals collected from Shidao, Shandong Province, China. A total of 202 alleles were detected at 25 loci. The numbers of alleles per locus ranged from 4 to 14, with an average of 8.08. The observed and expected heterozygosities varied from 0.2917 to 1.000 and from 0.3570 to 0.9002, respectively. After sequential Bonferroni correction for multiple tests, only one locus was found to deviate from Hardy-Weinberg equilibrium. Twenty-five EST-SSR markers showed a high rate of across-species transferability (100%) in Scapharca subcrenata and a low rate of across-genus transferability (20%) in Tegillarca granosa. These EST-SSRs will be helpful for QTL mapping, molecular breeding and investigation of population genetic diversity in ark shell S. broughtonii and other Scapharca species.
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Zhu
authors.forename: Chun-Ya
authors.initials: CY
authors.lastname: Zhou
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Fan
authors.forename: Zhi-Meng
authors.initials: ZM
authors.lastname: Zhuang
mesh_terms: D000483:Alleles; D000818:Animals; D049893:Arcidae / Q000235:genetics*; D001483:Base Sequence; D015415:Biomarkers / Q000032:analysis; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D020224:Expressed Sequence Tags; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D049894:Scapharca / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D015415:Biomarkers; D004247:DNA