title: Light-emitting diode induced fluorescence (LED-IF) detection design for a pen-shaped cartridge based single capillary electrophoresis system.
abstract: CGE is a well-established separation technique for the analysis of biologically important molecules such as nucleic acids. The inherent high resolving power, rapid analysis times, excellent detection sensitivity, and quantification capabilities makes this method favorable compared to conventional manual polyacrylamide and agarose slab gel electrophoresis techniques. In this paper we introduce a novel single-channel capillary gel electrophoresis system with LED-induced fluorescence detection also utilizing a compact pen-shaped capillary cartridge design for automatic analysis of samples from a 96-well plate. To evaluate the suitability of the system, 1000 genomic DNA(gDNA) samples were analyzed in gel filled capillaries and detected by the microball ended excitation and emission optical fiber based LED-induced fluorescence detection system. Excellent migration time reproducibility of RSD <0.75% was obtained over the course of 1000 runs. The system rapidly distinguished between intact and degraded gDNA samples, therefore provided important information if they could be used for downstream quantitative PCR processing where high-quality intact gDNA was key. We envision that this novel system design will rapidly find new applications in both research and clinical diagnostic laboratories as a highly sensitive and easy to use bio-analytical approach.
authors.affiliation: Horváth Laboratory of Bioseparation Sciences, University of Debrecen, Hungary.
authors.forename: Márta
authors.initials: M
authors.lastname: Kerékgyártó
authors.forename: Tamás
authors.initials: T
authors.lastname: Kerekes
authors.forename: Eric
authors.initials: E
authors.lastname: Tsai
authors.forename: Varoujan D
authors.initials: VD
authors.lastname: Amirkhanian
authors.forename: András
authors.initials: A
authors.lastname: Guttman
mesh_terms: D004247:DNA / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D055100:Optical Fibers; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
chemical_list: D004247:DNA