title: An electrochemiluminescent DNA sensor based on nano-gold enhancement and ferrocene quenching.
abstract: An electrochemiluminescent DNA (ECL-DNA) sensor based on nano-gold signal enhancement (i.e. gold nanoparticles, GNP) and ferrocene signal quenching was investigated. The Au electrode was first modified with GNPs through electrodeposition method, followed by subsequent immobilization of single-stranded probe DNA labeled with ruthenium complex. The resulting sensor produced a higher ECL signal due to its higher density of self-assembled probe DNAs on the surface. Upon the hybridization of probe DNA with complementary target DNA labeled with ferrocene, ECL intensity decreased significantly due to spatial separation of ECL label from the electrode surface. As a result, the ECL signal was simultaneously quenched by ferrocene. The effects of both nano-gold electrodeposition time and ferrocene on the performance of ECL-DNA sensor were studied in detail and possible reasons for these effects were suggested as well. The reported ECL-DNA sensor showed great sensitivity and may provide an alternative approach for DNA detection in diagnostics and gene analysis.
authors.affiliation: Anhui Key Laboratory of Chemo-Biosensing, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
authors.forename: Wu
authors.initials: W
authors.lastname: Yao
authors.forename: Lun
authors.initials: L
authors.lastname: Wang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.forename: Le
authors.initials: L
authors.lastname: Pan
authors.forename: Nannan
authors.initials: N
authors.lastname: Xing
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004566:Electrodes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005296:Ferrous Compounds / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D000075163:Metallocenes; D036103:Nanotechnology / Q000295:instrumentation; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
chemical_list: D005296:Ferrous Compounds; D000075163:Metallocenes; D006046:Gold; D004247:DNA; C004998:ferrocene