title: A novel method for the labelling of peptides and proteins through a bioorthogonal Staudinger reaction by using 2-cyanoethyl phosphoramidites.
abstract: Chemoselective modification of biomolecules: The reaction between 2-cyanoethyl phosphoramidites and azides is economical and can be performed in different solvents, including aqueous buffers. The course of the reaction with azido-modified amino acids, peptides or proteins and different label molecules was followed by (31)P NMR spectroscopy.
authors.affiliation: Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Freiburg 79104, Germany.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Uzagare
authors.forename: Iris
authors.initials: I
authors.lastname: Claussnitzer
authors.forename: Michael
authors.initials: M
authors.lastname: Gerrits
authors.forename: Willi
authors.initials: W
authors.lastname: Bannwarth
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D001386:Azides / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009943:Organophosphorus Compounds / Q000737:chemistry*; D010445:Peptide Elongation Factors / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D000596:Amino Acids; D001386:Azides; D029968:Escherichia coli Proteins; D009943:Organophosphorus Compounds; D010445:Peptide Elongation Factors; D010455:Peptides; C023512:elongation factor Ts; C434331:phosphoramidite