title: Fatty acid rich effluent from acidogenic biohydrogen reactor as substrate for lipid accumulation in heterotrophic microalgae with simultaneous treatment.
abstract: Acid-rich effluent generated from acidogenic biohydrogen production process was evaluated as substrate for lipid synthesis by integrating with heterotrophic cultivation of mixed microalgae. Experiments were performed both with synthetic volatile fatty acids (SVFA) and fermented fatty acids (FFA) from biohydrogen producing reactor. Fatty acid based platform evidenced significant influence on algal growth as well as lipid accumulation by the formation of triglycerides through fatty acid synthesis. Comparatively FFA documented higher biomass and lipid productivity (1.42mg/ml (wet weight); 26.4%) than SVFAs ((HAc+HBu+HPr), 0.60mg/ml; 23.1%). Lipid profiles varied with substrates and depicted 18 types of saturated and unsaturated fatty acids with wide fuel and food characteristics. The observed higher concentrations of Chl b over Chl a supports the biosynthesis of triacylglycerides. Microalgae diversity visualized the presence of lipid accumulating species viz., Scenedesmus sp. and Chlorella sp. Integration of microalgae cultivation with biohydrogen production showed lipid productivity for biodiesel production along with additional treatment.
authors.affiliation: Bioengineering and Environmental Centre (BEEC), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad 500607, India. vmohan_s@yahoo.com
authors.forename: S
authors.initials: S
authors.lastname: Venkata Mohan
authors.forename: M
authors.initials: M
authors.lastname: Prathima Devi
mesh_terms: D000143:Acids / Q000378:metabolism; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000379:methods*; D002734:Chlorophyll / Q000378:metabolism; D004951:Esterification; D005227:Fatty Acids / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000032:analysis; D052836:Heterotrophic Processes; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D010084:Oxidation-Reduction; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000737:chemistry; D018508:Water Purification
chemical_list: D000143:Acids; D002734:Chlorophyll; D005227:Fatty Acids; D005232:Fatty Acids, Volatile; D006859:Hydrogen; D008055:Lipids; D062065:Wastewater