title: Selection of candidate radiation biomarkers in the serum of rats exposed to gamma-rays by GC/TOFMS-based metabolomics.
abstract: In the study, gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) techniques coupled with principal components analysis (PCA) were used to investigate metabolite perturbations in the serum of the rats exposed to 0.75, 3 or 8 Gy gamma rays. Male standard deviation rats were gamma-irradiated at doses of 0.75, 3 and 8 Gy (1.9 Gy min(-1)) or sham-irradiated. Serum samples were collected over the first 24 h under the exposure to irradiation in order to analyse the samples by GC/TOFMS. And multivariate data were analysed by PCA. The composition of metabolites in serum yielded distinct metabolomic phenotypes for 0.75, 3 and 8 Gy at 24 h after irradiation. Nine serum metabolites were significantly altered as a result of radiation exposure. Up-regulated metabolites included inositol, serine, lysine, glycine, threonine and glycerol; down-regulated metabolites included isocitrate, gluconic acid and stearic acid. The nine metabolites were significantly altered after ionising radiation for they may be the potential biomarkers for the diagnosis of radiation injury.
authors.affiliation: Anhui Medical University, Hefei 230032, China.
authors.forename: Haixiang
authors.initials: H
authors.lastname: Liu
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Wang
authors.forename: Xueqing
authors.initials: X
authors.lastname: Zhang
authors.forename: Yulei
authors.initials: Y
authors.lastname: Qiao
authors.forename: Shengming
authors.initials: S
authors.lastname: Wu
authors.forename: Fangting
authors.initials: F
authors.lastname: Dong
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D055432:Metabolomics; D025341:Principal Component Analysis; D011832:Radiation Injuries / Q000097:blood / Q000175:diagnosis*; D051381:Rats
chemical_list: D015415:Biomarkers