title: [Concentration increase in Hcy and anti-CCP antibody in the serum of depression rat model induced by chronic unpredictable mild stress].
abstract: OBJECTIVE
  To observe the change of serum homocysteine (Hcy) and anti-citrullinated peptide (CCP) antibody concentration in depression rat model induced by chronic unpredictable mild stress (CUMS), and to explore the immunologic mechanism of depression and the relation between depression and its autoimmunity.
  
  
  METHODS
  Sixty adult male SD rats were randomly divided into 2 groups, 30 rats in each group, which were divided into 3 subgroups: a normal control group, a model group and a fluoxetinetreated group. The depression rat model was established under CUMS and seperated feeding, after which, open field, sugar consumption and forced swimming test were applied in the first group. After the blood was taken in the second group of rats through heart puncture, the level of serum Hcy was detected by enzymatic cycling assay and serum anti-CCP antibody by ELISA.
  
  
  RESULTS
  Compared with the control group and the fluoxetine treatment group, spontaneous activity and sucrose consumption and preference percentage of the rats in the model group significantly reduced, while the immobility time in forced swimming test and the level of Hcy and anti-CCP antibody in the rat serum significantly increased.
  
  
  CONCLUSIONS
  Immunity inflammation and autoimmune reaction exist in CUMS depression model rats, and fluoxetine treatment can improve these immune response.
authors.affiliation: Department of Clinical Laboratory, Second Xianya Hospital, Central South University, Changsha 410011, China. tayame139@yahoo.com.cn
authors.forename: Yamei
authors.initials: Y
authors.lastname: Tang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Lixin
authors.initials: L
authors.lastname: Qin
authors.forename: Rensheng
authors.initials: R
authors.lastname: Zhang
authors.forename: Ruohong
authors.initials: R
authors.lastname: Chen
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood*; D003863:Depression / Q000188:drug therapy / Q000209:etiology / Q000276:immunology*; D004195:Disease Models, Animal; D005473:Fluoxetine / Q000627:therapeutic use; D006710:Homocysteine / Q000097:blood*; D008297:Male; D010456:Peptides, Cyclic / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D010456:Peptides, Cyclic; C487763:cyclic citrullinated peptide; D005473:Fluoxetine; D006710:Homocysteine