title: The functional property of egg yolk phosvitin as a melanogenesis inhibitor.
abstract: Phosvitin is a phosphoglycoprotein present in egg yolk. More than half of the amino acids in phosvitin molecule are serine, of which >90% are phosphorylated. Therefore, phosvitin has a strong metal binding capability. The aim of this study was to investigate the effect of phosvitin on the inhibition of melanogenesis in melanoma cells. The results showed that phosvitin inhibited the activity of mushroom tyrosinase. Addition of phosvitin at a concentration of 50μg/ml, to B16F10 melanoma cells inhibited tyrosinase activity by approximately 42% and melanin synthesis by 17% compared to those in a control without phosvitin. Phosvitin inhibited the expression of tyrosinase, tyrosinase-related protein 1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF) in B16F10 melanoma cells. In addition, phosvitin reduced the cellular cAMP concentration in B16F10 melanoma cells. These results indicate that phosvitin has the potential to be used as a melanogenesis inhibitor in the food and cosmetics industry.
authors.affiliation: Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Samooel
authors.initials: S
authors.lastname: Jung
authors.forename: Dong Hee
authors.initials: DH
authors.lastname: Kim
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Son
authors.forename: Kichang
authors.initials: K
authors.lastname: Nam
authors.forename: Dong Uk
authors.initials: DU
authors.lastname: Ahn
authors.forename: Cheorun
authors.initials: C
authors.lastname: Jo
mesh_terms: D000363:Agaricales / Q000201:enzymology; D000818:Animals; D002460:Cell Line; D002645:Chickens; D015536:Down-Regulation / Q000187:drug effects; D004530:Egg Yolk / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005656:Fungal Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D010774:Phosvitin / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D008543:Melanins; D010774:Phosvitin; D014442:Monophenol Monooxygenase