title: Myo9B is associated with an increased risk of Barrett's esophagus and esophageal adenocarcinoma.
abstract: BACKGROUND
  Reflux esophagitis (RE) and Barrett's esophagus (BE) are predisposing factors for development of esophageal adenocarcinoma (EAC), the solid tumor with the fastest rising incidence in the Western world. This RE-BE-EAC cascade involves multiple host factors and consequently multiple genes. Polymorphisms in the 3' region of myosin IXB (Myo9B) are associated with chronic inflammatory gastrointestinal disorders like celiac disease and ulcerative colitis, assuming that variation in Myo9B influences the intestinal permeability.
  
  
  OBJECTIVE
  To determine esophageal expression and the genetic variation of the Myo9B gene in the RE-BE-EAC cascade.
  
  
  METHODS
  DNA from 886 Caucasian participants (198 non-reflux controls, 305 RE, 254 BE, 129 EAC) was collected for the determination of the Myo9B gene polymorphism (rs2305764). Esophageal Myo9B expression was determined on biopsies from normal, RE, BE and EAC epithelium.
  
  
  RESULTS
  Genotype G/G was more common in BE (p = 0.032) and EAC (p = 0.046), but not in RE (p = 0.126) compared with the control group. Cytoplasmic Myo9B expression was determined in RE, BE and EAC, but most prominent in epithelial cells of BE and EAC.
  
  
  CONCLUSIONS
  Genetic variation of Myo9B may play a role in the etiology of BE and EAC by increasing the permeability of the epithelial barrier.
authors.affiliation: Department of Gastroenterology and Hepatology, Erasmus MC - University Medical Center, Rotterdam, The Netherlands.
authors.forename: Vivianda
authors.initials: V
authors.lastname: Menke
authors.forename: Katinka P M
authors.initials: KP
authors.lastname: Van Zoest
authors.forename: Leon M G
authors.initials: LM
authors.lastname: Moons
authors.forename: Raymond G J
authors.initials: RG
authors.lastname: Pot
authors.forename: Peter D
authors.initials: PD
authors.lastname: Siersema
authors.forename: Ernst J
authors.initials: EJ
authors.lastname: Kuipers
authors.forename: Johannes G
authors.initials: JG
authors.lastname: Kusters
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D001471:Barrett Esophagus / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D016001:Confidence Intervals; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism; D004942:Esophagitis, Peptic / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009218:Myosins / Q000235:genetics* / Q000378:metabolism; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011230:Precancerous Conditions / Q000235:genetics* / Q000378:metabolism
chemical_list: C113469:myosin IXB; D009218:Myosins