title: Sanguinarine inhibits growth of human cervical cancer cells through             the induction of apoptosis.
abstract: Sanguinarine, a natural benzophenanthridine alkaloid, has been shown to             possess anticancer activity in vitro and in vivo. In the present study, we demonstrated             that sanguinarine caused a dose-dependent inhibition of growth in HeLa and SiHa             human cervical cancer cells, i.e., 2.43 µmol/l (IC50) in HeLa cells and 3.07 µmol/l             in SiHa cells. Cell cycle analysis revealed that sanguinarine significantly increased             the sub-G1 population, from 1.7 to 59.7% in HeLa cells and from 1.7 to 41.7% in             SiHa cells. Sanguinarine caused a dose-dependent decrease in Bcl-2 and NF-κB protein             expression and a significant increase in Bax protein expression. Our findings             indicate that sanguinarine as an effective anticancer drug candidate inhibits             the growth of cervical cancer cells through the induction of apoptosis.
authors.affiliation: School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu 215123, PR China.
authors.forename: Jia-Ying
authors.initials: JY
authors.lastname: Xu
authors.forename: Qing-Hui
authors.initials: QH
authors.lastname: Meng
authors.forename: Yu
authors.initials: Y
authors.lastname: Chong
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.forename: Lin
authors.initials: L
authors.lastname: Zhao
authors.forename: Eliot M
authors.initials: EM
authors.lastname: Rosen
authors.forename: Saijun
authors.initials: S
authors.lastname: Fan
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053119:Benzophenanthridines / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology*; D016328:NF-kappa B / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology
chemical_list: D000890:Anti-Infective Agents; D053119:Benzophenanthridines; D007546:Isoquinolines; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; C005705:sanguinarine