title: Killer toxin from a novel killer yeast Pichia kudriavzevii RY55 with idiosyncratic antibacterial activity.
abstract: The killer phenomenon of yeast may have technological implications in many areas like beverage fermentation, food technology, biological control in agriculture, and in medicine. In the present study the killer phenomenon in Pichia kudriavzevii (P. kudriavzevii RY55) is being reported for the first time. The P. kudriavzevii RY55 toxin exhibited excellent antibacterial activity against several pathogens of human health significance such as Escherichia coli, Enterococcus faecalis, Klebsiella sp., Staphylococcus aureus, Pseudomonas aeruginosa and Pseudomonas alcaligenes. Killer toxin was purified to homogeneity by using ammonium sulphate precipitation and ion exchange chromatography and characterized for few properties. P. kudriavzevii RY55 killer toxin may be of vast significance in the development of novel antimicrobial chemotherapeutic agents, new bio-based safer candidates for food preservation and biocontrol, and starter cultures for fermentation industries.
authors.affiliation: School of Biotechnology, University of Jammu, Jammu, India. bajajbijenderk@gmail.com
authors.forename: Bijender Kumar
authors.initials: BK
authors.lastname: Bajaj
authors.forename: Sandeepu
authors.initials: S
authors.lastname: Raina
authors.forename: Satbir
authors.initials: S
authors.lastname: Singh
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007709:Klebsiella / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D009183:Mycotoxins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D010843:Pichia / Q000737:chemistry* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D044202:Pseudomonas alcaligenes / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D009183:Mycotoxins