title: Association of ADAMTS5 gene polymorphisms with osteoarthritis in Chinese Han population: a community-based case-control study.
abstract: Aggrecanase-2 (ADAMTS5) is reported to play essential roles in the pathophysiology of osteoarthritis (OA). To explore the relationship between ADAMTS5 gene polymorphisms and primary OA, we conducted a community-based case-control study. A total of 732 community residents aged 40-84 years participated in the community-based study in Northeast China. After taking physical examination and radiographic examination, 420 persons of the residents were diagnosed OA (216 women and 204 men). The other 312 individuals without any symptoms of osteoarthritis or signs in the radiographs (156 women and 156 men) were considered as healthy controls. After obtaining the DNA of patients and control group, genotypes of the ADAMTS5 gene polymorphisms were determined by polymerase chain reaction (PCR) followed by restriction enzyme digestion (HAEIII for P692L in exon 7 and BSRBI for R614H in exon 5). The numbers of patients with different OA subtypes were also calculated. The genotype and allele frequency of for the exon 5C/T BSRBI polymorphism was significantly different between OA patients and control individuals (P = 0.001, OR = 0.701, 95% CI = 0.569-0.863). This difference was more obvious in cervical OA patients (P = 0.001, OR = 0.664, 95% CI = 0.521-0.847). The mutation type of exon 5C/T BSRBI polymorphism would be a protective factor for OA especially for cervical OA. Our results suggest that the ADAMTS5 gene polymorphisms may contribute to the susceptibility of osteoarthritis in the Chinese Han population.
authors.affiliation: Department of the 2nd Affiliated Hospital, Harbin Medical University, No. 194 Baojian Road, Harbin, Heilongjiang Province, People's Republic of China.
authors.forename: Jiaao
authors.initials: J
authors.lastname: Gu
authors.forename: Jiesheng
authors.initials: J
authors.lastname: Rong
authors.forename: Fulin
authors.initials: F
authors.lastname: Guan
authors.forename: Liying
authors.initials: L
authors.lastname: Jiang
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Shuqing
authors.initials: S
authors.lastname: Tao
authors.forename: Guofa
authors.initials: G
authors.lastname: Guan
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.forename: Tianzun
authors.initials: T
authors.lastname: Tao
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D000071116:ADAMTS5 Protein; D000328:Adult; D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D051722:ADAM Proteins; D000071116:ADAMTS5 Protein; C121532:ADAMTS5 protein, human