title: Transcriptome analysis of Nicotiana tabacum infected by Cucumber mosaic virus during systemic symptom development.
abstract: Virus infection of plants may induce a variety of disease symptoms. However, little is known about the molecular mechanism of systemic symptom development in infected plants. Here we performed the first next-generation sequencing study to identify gene expression changes associated with disease development in tobacco plants (Nicotiana tabacum cv. Xanthi nc) induced by infection with the M strain of Cucumber mosaic virus (M-CMV). Analysis of the tobacco transcriptome by RNA-Seq identified 95,916 unigenes, 34,408 of which were new transcripts by database searches. Deep sequencing was subsequently used to compare the digital gene expression (DGE) profiles of the healthy plants with the infected plants at six sequential disease development stages, including vein clearing, mosaic, severe chlorosis, partial and complete recovery, and secondary mosaic. Thousands of differentially expressed genes were identified, and KEGG pathway analysis of these genes suggested that many biological processes, such as photosynthesis, pigment metabolism and plant-pathogen interaction, were involved in systemic symptom development. Our systematic analysis provides comprehensive transcriptomic information regarding systemic symptom development in virus-infected plants. This information will help further our understanding of the detailed mechanisms of plant responses to viral infection.
authors.affiliation: Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Du
authors.forename: Jun
authors.initials: J
authors.lastname: Kong
authors.forename: Ling-Na
authors.initials: LN
authors.lastname: Chen
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Qiu
authors.forename: Gui-Fen
authors.initials: GF
authors.lastname: Li
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Meng
authors.forename: Shui-Fang
authors.initials: SF
authors.lastname: Zhu
mesh_terms: D017799:Cucumovirus / Q000235:genetics*; D016208:Databases, Factual; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D008957:Models, Genetic; D015233:Models, Statistical; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000378:metabolism; D010944:Plants / Q000821:virology*; D012313:RNA / Q000378:metabolism; D034622:RNA Interference; D017423:Sequence Analysis, RNA; D012984:Software; D013997:Time Factors; D014026:Nicotiana / Q000378:metabolism* / Q000821:virology
chemical_list: D012313:RNA