title: Single-cell electric lysis on an electroosmotic-driven microfluidic chip with arrays of microwells.
abstract: Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-μm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.
authors.affiliation: Department of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovation, National Chung Cheng University, Chia Yi, Taiwan. imecpj@ccu.edu.tw
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Jen
authors.forename: Tamara G
authors.initials: TG
authors.lastname: Amstislavskaya
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Liu
authors.forename: Ju-Hsiu
authors.initials: JH
authors.lastname: Hsiao
authors.forename: Yu-Hung
authors.initials: YH
authors.lastname: Chen