title: [Immunophenotypic analysis of hematogones in patients with hematological malignancies].
abstract: We studied immunophenotypic analysis of hematogones by flow cytometry. A total of 102 specimens from 93 patients with acute leukemia (52 specimens), myelodysplastic syndromes (4), or malignant lymphoma (46) were analyzed between April and August, 2011. Hematogones were detected in 55 specimens and highly identified in patients with acute myeloid leukemia in remission and B cell lymphoma. Stage 1 (CD34(+)CD20(-)) and stage 2/3 (CD34(-)CD20(+)) were detected in 9.9% and 52.7%, respectively. In addition, the intermediate type (CD34(+)CD20(+)) was identified in 37.4%. All specimens of stage 3 in bright CD45 expression were positive for CD5 and included CD5(+)CD23(-)CD11c(-), 11.1%, CD5(+)CD23(+)CD11c(-), 85.2%, and CD5(+)CD23(+)CD11c(+), 3.7%. These findings suggest that hematogones with unreported immunophenotypes may exist and the appearance of hematogones in hematologic malignancies may be relatively frequent.
authors.affiliation: Division of Clinical Laboratory, Kanagawa Cancer Center, Japan.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Kobayashi
authors.forename: Risa
authors.initials: R
authors.lastname: Kumagai
authors.forename: Akiko
authors.initials: A
authors.lastname: Omiya
authors.forename: Hideki
authors.initials: H
authors.lastname: Tanno
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Ishii
authors.forename: Wataru
authors.initials: W
authors.lastname: Yamamoto
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Takasaki
authors.forename: Rika
authors.initials: R
authors.lastname: Sakai
authors.forename: Ayumi
authors.initials: A
authors.lastname: Numata
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Tanaka
authors.forename: Heiwa
authors.initials: H
authors.lastname: Kanamori
authors.forename: Shigeki
authors.initials: S
authors.lastname: Motomura
authors.forename: Atsuo
authors.initials: A
authors.lastname: Maruta
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD; D001853:Bone Marrow / Q000473:pathology; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000175:diagnosis* / Q000276:immunology* / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D008297:Male; D008875:Middle Aged; D054448:Precursor Cells, B-Lymphoid / Q000145:classification / Q000276:immunology* / Q000473:pathology; D055815:Young Adult
chemical_list: D015703:Antigens, CD