title: 2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside ameliorates vascular senescence and improves blood flow involving a mechanism of p53 deacetylation.
abstract: OBJECTIVE
  2,3,5,4'-tetrahydroxystilbene-2-O-β-d-glucoside (THSG), a resveratrol analog with glucoside, has been shown in various studies to inhibit proliferation of vascular smooth muscle cells, attenuate inflammation, and prevent vascular endothelial dysfunction. In the study, we examined the effects of THSG on vascular senescence and blood flow.
  
  
  RESULTS
  Oral administration of THSG for 14 weeks, resulted in notable increases in blood flow in spontaneously hypertensive rats (SHRs); and effective inhibition of vascular senescence as indicated by senescence-associated β-galactosidase (SA-β-gal) staining, phosphorylation of γH2AX observed by stain analysis of immunofluorescence, and K373 acetylation of p53 in the aortic arches of SHRs. Oral administration of THSG also induced eNOS expression and urinary NOx production. THSG weekly activated SIRT1 activity, stimulated eNOS promoter reporter gene activity, and ameliorated H(2)O(2)-induced cellular senescence and K373 acetylation of p53 in cultured human umbilical vein endothelial cells (HUVECs).
  
  
  CONCLUSIONS
  THSG improves blood flow and ameliorates vascular senescence by increasing eNOS expression and Sirt1 activity and decreasing acetylation of p53 at K373 site, at least in part, both in vitro and in vivo.
authors.affiliation: Murad Research Institute for Modernized Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Rd., Pudong New Area, Shanghai 201203, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Han
authors.forename: Shuang
authors.initials: S
authors.lastname: Ling
authors.forename: Woting
authors.initials: W
authors.lastname: Gan
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Ju
authors.initials: J
authors.lastname: Duan
authors.forename: Jin-Wen
authors.initials: JW
authors.lastname: Xu
mesh_terms: D000375:Aging; D000818:Animals; D002339:Carotid Arteries / Q000502:physiology; D016922:Cellular Senescence / Q000187:drug effects*; D005960:Glucosides / Q000494:pharmacology*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008297:Male; D052250:Nitric Oxide Synthase Type III / Q000096:biosynthesis; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012039:Regional Blood Flow / Q000187:drug effects*; D056564:Sirtuin 1 / Q000096:biosynthesis; D013267:Stilbenes / Q000494:pharmacology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D005960:Glucosides; D013267:Stilbenes; D016159:Tumor Suppressor Protein p53; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D056564:Sirtuin 1