title: Prevalence and genotypes of Giardia duodenalis from dogs in Spain: possible zoonotic transmission and public health importance.
abstract: The prevalence of Giardia duodenalis was determinate in faecal samples from dogs and cats in Madrid, Spain and molecular characterisation of isolates. A total of 604 and 144 faecal samples from dogs and cats, respectively, were analysed by routine coprological methods. The prevalence of G. duodenalis was 16.4 % (99/604) in dogs and 4.2 % (6/144) in cats. Sixty-four G. duodenalis isolates (63 from dogs and 1 from a cat) were characterised using glutamate dehydrogenase and β-giardin genes by PCR-RFLP. The single cat sample showed a mixed infection by assemblages A + F. The assemblages found in the dog samples were A, B, C, D and E, both as single and as mixed infections. The zoonotic assemblages A and B were found in 56 (88.8 %) G. duodenalis-positive samples with 15.9 % of samples having assemblage A (10/63) and 73 % of samples with assemblage B (46/63), indicating high potential zoonotic risk and public health significance.
authors.affiliation: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
authors.forename: Diana
authors.initials: D
authors.lastname: Dado
authors.forename: Ana
authors.initials: A
authors.lastname: Montoya
authors.forename: María Alejandra
authors.initials: MA
authors.lastname: Blanco
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Miró
authors.forename: José María
authors.initials: JM
authors.lastname: Saugar
authors.forename: Begoña
authors.initials: B
authors.lastname: Bailo
authors.forename: Isabel
authors.initials: I
authors.lastname: Fuentes
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000453:epidemiology / Q000469:parasitology; D002415:Cats; D016172:DNA Fingerprinting; D004283:Dog Diseases / Q000453:epidemiology* / Q000469:parasitology; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D005969:Glutamate Dehydrogenase; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D015800:Protozoan Proteins / Q000235:genetics; D013030:Spain / Q000453:epidemiology
chemical_list: D015800:Protozoan Proteins; D005969:Glutamate Dehydrogenase