title: Expression and roles of Wilms' tumor 1-associating protein in glioblastoma.
abstract: Glioblastoma is a diffusely growing malignant brain tumor and among the most aggressive of all tumors. Wilms' tumor 1-associating protein (WTAP) is a nuclear protein that has been associated with regulation of proliferation and apoptosis. Although its dynamic expression and physiological functions in vascular cells have been reported, those in other cells are largely unknown. Here, we show for the first time that WTAP is overexpressed in glioblastoma. Moreover we found that WTAP regulates migration and invasion of glioblatoma cells. Specific knockdown by siRNA or overexpression by cDNA regulated migration and invasion of cancer cells. In xenograft study, WTAP overexpression made cancer cells more tumorigenic. In the investigation for its underlying mechanism, we found that the activity of epidermal growth factor receptor can be regulated by WTAP. These results reveal a novel function of WTAP and suggest its clinical application.
authors.affiliation: Department of Anatomy, Pusan National University, Beomeo-Ri, Mulgeum-Eup, Yangsan, Korea.
authors.forename: Du-Il
authors.initials: DI
authors.lastname: Jin
authors.forename: Sang Weon
authors.initials: SW
authors.lastname: Lee
authors.forename: Myoung-Eun
authors.initials: ME
authors.lastname: Han
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Seon-Ae
authors.initials: SA
authors.lastname: Seo
authors.forename: Gi-Yeong
authors.initials: GY
authors.lastname: Hur
authors.forename: Shin
authors.initials: S
authors.lastname: Jung
authors.forename: Bong-Seon
authors.initials: BS
authors.lastname: Kim
authors.forename: Sae-Ock
authors.initials: SO
authors.lastname: Oh
mesh_terms: D000818:Animals; D017209:Apoptosis; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D002465:Cell Movement; D018076:DNA, Complementary / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D000072260:RNA Splicing Factors; D034741:RNA, Small Interfering / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D009687:Nuclear Proteins; D000072260:RNA Splicing Factors; D034741:RNA, Small Interfering; C419554:WTAP protein, human; D066246:ErbB Receptors