title: Are alsin and spartin novel interaction partners?
abstract: Mutations in ALS2 gene/alsin are associated with recessive forms of motor neuron disorders including Juvenile Amyotrophic Lateral Sclerosis (JALS), Infantile-onset Ascending Hereditary Spastic Paraplegia (IAHSP) and Juvenile Primary Lateral Sclerosis (JPLS). In this study, we show that alsin and another MND-linked protein, spartin are related to each other both at mRNA and protein levels in Neuro2a cells. We observed significant alterations in spartin expression in alsin knock-down conditions. We further found that both proteins colocalize in N2a cells and spartin isoform-a precipitates with alsin in the same protein complex. In the light of these results we suggest that alsin and spartin may interact each other physically.
authors.affiliation: Department of Molecular Biology and Genetics, Neurodegeneration Research Laboratory (NDAL), Boğaziçi University, Istanbul, Turkey.
authors.forename: Gönenç
authors.initials: G
authors.lastname: Çobanoğlu
authors.forename: Mehmet
authors.initials: M
authors.lastname: Ozansoy
authors.forename: A Nazlı
authors.initials: AN
authors.lastname: Başak
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D016472:Motor Neuron Disease / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C512358:Als2 protein, mouse; D002352:Carrier Proteins; D020662:Guanine Nucleotide Exchange Factors; D020033:Protein Isoforms; D012333:RNA, Messenger; C562344:SPG20 protein, mouse