title: Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium.
abstract: The extraction residue of the Ganoderma fruiting body, named sacchachitin, has been demonstrated to have the potential to enhance cutaneous wound healing by inducing cell proliferation. In this study, a nanogel formed from micronized sacchachitin (mSC) was investigated for the potential treatment of superficial chemical corneal burns. Reportedly, mSC has been produced successfully and its chemical properties confirmed, and physical and rheological properties characterized. An in vitro cell proliferation study has revealed that at the concentrations of 200, 300, and 400 microg/mL, mSC nanogel significantly increased Statens Seruminstitut rabbit corneal (SIRC) cell proliferation after 24 hours of incubation. In cell migration assay, migration of SIRC cell to wound closure was observed after 24 hours of incubation with the addition of 200 microg/mL mSC of nanogel. In an animal study, acceleration of corneal wound healing was probably due to the inhibition of proteolysis. In conclusion, the findings of this study substantiate the potential application of sacchachitin in the form of mSC nanogel for the treatment of superficial corneal injuries.
authors.affiliation: Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, Taipei, Republic of China.
authors.forename: Ray-Neng
authors.initials: RN
authors.lastname: Chen
authors.forename: Lin-Wen
authors.initials: LW
authors.lastname: Lee
authors.forename: Ling-Chun
authors.initials: LC
authors.lastname: Chen
authors.forename: Hsiu-O
authors.initials: HO
authors.lastname: Ho
authors.forename: Shiao-Chuan
authors.initials: SC
authors.lastname: Lui
authors.forename: Ming-Thau
authors.initials: MT
authors.lastname: Sheu
authors.forename: Ching-Hua
authors.initials: CH
authors.lastname: Su
mesh_terms: D000818:Animals; D002057:Burns, Chemical / Q000188:drug therapy* / Q000503:physiopathology; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D019573:Epithelium, Corneal / Q000187:drug effects / Q000293:injuries* / Q000378:metabolism*; D005126:Eye Burns / Q000188:drug therapy* / Q000503:physiopathology; D005782:Gels / Q000627:therapeutic use; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D049329:Nanostructures / Q000627:therapeutic use; D011817:Rabbits; D020940:Reishi; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
chemical_list: D004365:Drugs, Chinese Herbal; D005782:Gels; D020780:Matrix Metalloproteinase 9