title: Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MS methods.
abstract: Peanut shell, a byproduct in oil production, is rich in natural antioxidants. Here, a rapid and efficient method using DPPH-HPLC-DAD-TOF/MS was used for the first time to screen antioxidants in peanut shell. The method is based on the hypothesis that upon reaction with 1, 1-diphenyl-2-picrylhydrazyl (DPPH), the peak areas of compounds with potential antioxidant activities in the HPLC chromatogram will be significantly reduced or disappeared, and the identity confirmation could be achieved by HPLC-DAD-TOF/MS technique. With this method, three compounds possessing potential antioxidant activities were found abundantly in the methanolic extract of peanut shell. They were identified as 5,7-dihydroxychromone, eriodictyol, and luteolin. The contents of these compounds were 0.59, 0.92, and 2.36 mg/g, respectively, and luteolin possessed the strongest radical scavenging capacity. DPPH-HPLC-DAD-TOF/MS assay facilitated rapid identification and determination of natural antioxidants in peanut shell, which may be helpful for value-added utilization of peanut processing byproducts.
authors.affiliation: Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
authors.forename: Jiying
authors.initials: J
authors.lastname: Qiu
authors.forename: Leilei
authors.initials: L
authors.lastname: Chen
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Zhu
authors.forename: Daijie
authors.initials: D
authors.lastname: Wang
authors.forename: Wenliang
authors.initials: W
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Liu
authors.forename: Fangling
authors.initials: F
authors.lastname: Du
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D010367:Arachis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D007220:Industrial Waste / Q000032:analysis; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
chemical_list: D000975:Antioxidants; D007220:Industrial Waste; D010936:Plant Extracts