title: Cell adhesion and migration on nanopatterned substrates and their effects on cell-capture yield.
abstract: With scanning electron microscopy analysis, we investigated the role of nanoscale topography on cellular activities; e.g. cell adhesion and spreading by culturing A549 cells (human lung carcinoma cell line cells) for 1-48 h on three sets of nanostructures; quartz nanopillars (QNPs), silicon nanopillars and silicon nanowire (SiNW) arrays, along with planar glass substrates. We found that cells on QNP arrays developed a longer shape than those on SiNW arrays. In addition, we studied how cell morphologies influence the cell-capture yield on the three sets of nanostructures. This research showed that the filopodial formations were directing the cell-capture yield on nanostructured substrates. This finding implies the possibility of using nanoscale topography features to control the filopodial formation including extension and migration from the cells. Using streptavidin-functionalized SiNW substrate, we further demonstrated a substantially higher yield (~91.8 ± 5.9%) than the planar glass wafers (~24.1 ± 7.5%) in the range of 200-3000 cells.
authors.affiliation: Basic Research Laboratory (BRL), Department of Semiconductor Science and Technology, Chonbuk National University, Jeonju, 561-756, Korea.
authors.forename: Dong-Joo
authors.initials: DJ
authors.lastname: Kim
authors.forename: Jin-Kyeong
authors.initials: JK
authors.lastname: Seol
authors.forename: Geehee
authors.initials: G
authors.lastname: Lee
authors.forename: Gil-Sung
authors.initials: GS
authors.lastname: Kim
authors.forename: Sang-Kwon
authors.initials: SK
authors.lastname: Lee
mesh_terms: D002448:Cell Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size; D018914:Cells, Immobilized / Q000166:cytology*; D005898:Glass; D006801:Humans; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D011791:Quartz; D012825:Silicon; D019809:Streptavidin; D013499:Surface Properties
chemical_list: D011791:Quartz; D019809:Streptavidin; D012825:Silicon