title: A new cytotoxic steroidal saponin from the rhizomes and roots of Smilax scobinicaulis.
abstract: A phytochemical investigation of the EtOH extract from the rhizomes and roots of Smilax scobinicaulis resulted in the isolation of a new isospirostanol-type steroidal saponin, namely (25 R)-5α-spirostan-3β,6β-diol 3-O-β-D-glucopyranosyl-(1 → 4)-[α-L-arabinopyranosyl-(1 → 6)]-β-D-glucopyranoside (1), along with four known steroidal saponins (2-5). The structures of these compounds were determined by 1D- and 2D-NMR spectroscopic analysis, FABMS and HR-ESI-MS as well as chemical degradation. The isolated saponins were evaluated for their in vitro cytotoxicity against A549, LAC and Hela human cancer cell lines, which demonstrated that only compound 1 possessed significant cytotoxic activity with IC₅₀ values of 3.70, 5.70 and 3.64 µM, respectively.
authors.affiliation: College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Cunli
authors.initials: C
authors.lastname: Zhang
authors.forename: Shixiu
authors.initials: S
authors.lastname: Feng
authors.forename: Lanxi
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Ren
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003603:Cytotoxins / Q000494:pharmacology; D000431:Ethanol; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D032281:Smilax / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D003603:Cytotoxins; D010936:Plant Extracts; D012503:Saponins; D013256:Steroids; D013778:Tetrazolium Salts; D013844:Thiazoles; D000431:Ethanol; C022616:thiazolyl blue