title: Comparative vesicle proteomics reveals selective regulation of protein expression in chestnut blight fungus by a hypovirus.
abstract: The chestnut blight fungus (Cryphonectria parasitica) and hypovirus constitute a model system to study fungal pathogenesis and mycovirus-host interaction. Knowledge in this field has been gained largely from investigations at gene transcription level so far. Here we report a systematic analysis of the vesicle proteins of the host fungus with/without hypovirus infection. Thirty-three differentially expressed protein spots were identified in the purified vesicle protein samples by two-dimensional electrophoresis and mass spectrometry. Down-regulated proteins were mostly cargo proteins involved in primary metabolism and energy generation and up-regulated proteins were mostly vesicle associated proteins and ABC transporter. A virus-encoded protein p48 was found to have four forms with different molecular mass in vesicles from the virus-infected strain. While a few of the randomly selected differentially expressed proteins were in accordance with their transcription profiles, majority were not in agreement with their mRNA accumulation patterns, suggesting that an extensive post-transcriptional regulation may have occurred in the host fungus upon a hypovirus infection.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources and Key Laboratory for Microbial and Plant Genetic Engineering, Ministry of Education, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
authors.forename: Jinzi
authors.initials: J
authors.lastname: Wang
authors.forename: Fangzhen
authors.initials: F
authors.lastname: Wang
authors.forename: Youjun
authors.initials: Y
authors.lastname: Feng
authors.forename: Ke
authors.initials: K
authors.lastname: Mi
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Jinjie
authors.initials: J
authors.lastname: Shang
authors.forename: Baoshan
authors.initials: B
authors.lastname: Chen
mesh_terms: D001203:Ascomycota / Q000378:metabolism* / Q000821:virology*; D005656:Fungal Proteins / Q000096:biosynthesis*; D015966:Gene Expression Regulation, Fungal; D054884:Host-Pathogen Interactions / Q000502:physiology*; D040901:Proteomics; D012328:RNA Viruses / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins