title: eNOS gene polymorphisms modify the association of PM(10) with oxidative stress.
abstract: Previous studies have suggested that air pollution increases various health outcomes through oxidative stress and oxidative stress-related genes modify the relationship between air pollution and health outcomes. Therefore, we evaluated the effect of PM(10) on the levels of malondialdehyde (MDA), oxidative stress biomarker, and the effect modification by genetic polymorphisms of eNOS, oxidative stress-related gene, in the 560 Korean elderly. We obtained urine samples repeatedly from participants during five medical examinations between 2008 and 2010 and all ambient air pollutant concentration data from the Korea National Institute of Environmental Research air quality monitoring system. We measured urinary levels of MDA to assess oxidative stress and genotyped eNOS (rs1799983, rs2853796, and rs7830). Mixed-effect model was used to estimate the effect of PM(10) on the level of oxidative stress biomarker and their modification by genotypes. PM(10) showed apparent positive effect on MDA level after adjusting for age, sex, BMI, cotinine level, temperature, dew point, levels of SO(2), O(3), NO(2), and CO, and season (p=0.0133). Moreover, the association of PM(10) with MDA was found only in participants with eNOS GG genotype for rs1799983 (p=0.0107), TT genotype for rs2853796 (p=0.0289), or GT genotype for rs7830 (p=0.0158) and in participants with a set of risky haplotypes (GTT, GTG, GGT, and TGT) (p=0.0093). Our results suggest that PM(10) affect oxidative stress in the elderly and eNOS genotype affect the oxidative stress level in regard of exposure to PM(10).
authors.affiliation: Institute of Environmental Medicine, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea. jhkim777@snu.ac.kr
authors.forename: Jin Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Yoon-Hyeong
authors.initials: YH
authors.lastname: Choi
authors.forename: Sanghyuk
authors.initials: S
authors.lastname: Bae
authors.forename: Hye-Yin
authors.initials: HY
authors.lastname: Park
authors.forename: Yun-Chul
authors.initials: YC
authors.lastname: Hong
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000393:Air Pollutants / Q000633:toxicity*; D015415:Biomarkers / Q000378:metabolism; D004784:Environmental Monitoring; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007723:Korea; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism / Q000652:urine; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D010316:Particle Size; D052638:Particulate Matter / Q000633:toxicity*; D020641:Polymorphism, Single Nucleotide
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D052638:Particulate Matter; D008315:Malondialdehyde; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III