title: Identity and public health potential of Cryptosporidium spp. in water buffalo calves in Egypt.
abstract: Little is known about the diversity and public health significance of Cryptosporidium species in water buffaloes. In this study, we examined the distribution of Cryptosporidium spp. in water buffalo calves in Egypt. Rectal fecal specimens from 179 calves and 359 adults were screened microscopically for Cryptosporidium oocysts using modified Ziehl-Neelsen stain. Cryptosporidium spp. in 17 microscopy-positive specimens from calves were genotyped by DNA sequence analysis of the small-subunit rRNA gene, and Cryptosporidium parvum was subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium ryanae was found in 10 specimens and C. parvum in 7 specimens, with the former belonging to the newly identified C. ryanae buffalo variant and the latter belonging to the subtypes IIdA20G1 (in 5 specimens) and IIaA15G1R1 (in 2 specimens). The prevailing occurrence of C. ryanae and the subtype family IId of C. parvum and the absence of C. bovis and C. andersoni represent some features of Cryptosporidium transmission in water buffaloes in Egypt.
authors.affiliation: Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA, USA.
authors.forename: Said
authors.initials: S
authors.lastname: Amer
authors.forename: Shereif
authors.initials: S
authors.lastname: Zidan
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Feng
authors.forename: Haileeyesus
authors.initials: H
authors.lastname: Adamu
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Lihua
authors.initials: L
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D003457:Cryptosporidiosis / Q000453:epidemiology / Q000662:veterinary*; D003458:Cryptosporidium / Q000145:classification / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D004534:Egypt / Q000453:epidemiology; D005243:Feces / Q000469:parasitology; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D011634:Public Health
chemical_list: D004275:DNA, Ribosomal