title: Rapid sequencing of a peptide containing a single disulfide bond using high-energy  collision-induced dissociation.
abstract: A peptide containing a single disulfide bond was sequenced using high-energy collision-induced dissociation (HE-CID) in conjunction with a high mass resolution time-of-flight  tandem mass spectrometer equipped with a matrix-assisted laser desorption/ionization source. This mass spectrometer, which has spiral ion  trajectory, allowed both high mass resolution and high precursor ion selectivity. It is difficult to obtain sufficient product ions from peptides containing disulfide bonds using HE-CID due to the single collision in the gas phase. To compensate for insufficient dissociation, the disulfide bond was cleaved via an in-source reduction process using 1,5-diaminonaphthalene, a reducing matrix. After applying the reduction in the ionization, subsequent sequencing using HE-CID provided the detailed structural information of the peptide containing the single disulfide bond.
authors.affiliation: School of Science, Osaka University, Toyonaka, Osaka, Japan.
authors.forename: Takuma
authors.initials: T
authors.lastname: Degawa
authors.forename: Shuichi
authors.initials: S
authors.lastname: Shimma
authors.forename: Michisato
authors.initials: M
authors.lastname: Toyoda
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites / Q000528:radiation effects; D004220:Disulfides / Q000737:chemistry* / Q000528:radiation effects; D020450:Heavy Ions; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000737:chemistry* / Q000528:radiation effects*; D011485:Protein Binding / Q000528:radiation effects; D020539:Sequence Analysis, Protein / Q000379:methods*
chemical_list: D004220:Disulfides; D010455:Peptides