title: Complete genome sequence of a Peste des petits ruminants virus recovered from wild bharal in Tibet, China.
abstract: For the first time, here we announce the complete genome sequence of a field isolate of Peste des petits ruminants virus (PPRV) derived from macerated rectal tissue of a free living bharal (Pseudois nayaur) that displayed clinical disease consistent with severe infection with PPRV. Further, we compare the full genome of this isolate, termed PPRV Tibet/Bharal/2008, with previously available PPRV genomes, including those of virus isolates from domestic small ruminants local to the area where the reported isolate was collected. The current sequence is phylogenetically classified as a lineage IV virus, sharing high levels of sequence identity with previously described Tibetan PPRV isolates. Indeed, across the entire genome, only 26 nucleotide differences (0.16% nucleotide variation) and, consequently, 9 amino acid changes were present compared to sequences of locally derived viruses.
authors.affiliation: China Animal Health and Epidemiology Center, Qingdao, Shandong, People's Republic of China.
authors.forename: Jingyue
authors.initials: J
authors.lastname: Bao
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Wang
authors.forename: Satya
authors.initials: S
authors.lastname: Parida
authors.forename: Chunju
authors.initials: C
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Wenji
authors.initials: W
authors.lastname: Zhao
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002681:China; D016678:Genome; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D009711:Nucleotides / Q000235:genetics; D029021:Peste-des-Petits-Ruminants / Q000821:virology*; D018107:Peste-des-petits-ruminants virus / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D012418:Ruminants / Q000235:genetics*; D017422:Sequence Analysis, DNA; D018609:Tibet
chemical_list: D009711:Nucleotides