title: Inhibitory effects of hydroxysafflor yellow A on the formation of advanced glycation end products in vitro.
abstract: To investigate the inhibitory effects of hydroxysafflor yellow A (HSYA) on the protein glycation in vitro. Using bovine serum albumin (BSA)-glucose assay, BSA-methylglyoxal (MGO) assay, and N-acetylglycyl-lysine methyl ester (G.K.) peptide-ribose assay, inhibitory effects of HSYA were investigated. Advanced glycation end products (AGEs) production was assessed by AGEs-specific fluorescence and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In BSA-glucose assay, HSYA concentration dependently decreased AGEs formation, with maximum inhibitory effects at 1 mM by 95%. Further more, HSYA also showed significant inhibitory effects on MGO-medicated protein modification and subsequent cross-linking of proteins. Finally, when co-incubated with G.K. peptide and ribose, HSYA exhibited its antiglycation effects, and the maximum inhibitory effects of HSYA at 1 mM were 84%. Overall, our present study provides the first evidence of the antiglycation effects of HSYA on AGEs formation in vitro.
authors.affiliation: Department of Pharmacy, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Ni
authors.forename: Zhengbing
authors.initials: Z
authors.lastname: Zhuge
authors.forename: Wenlu
authors.initials: W
authors.lastname: Li
authors.forename: Huimin
authors.initials: H
authors.lastname: Xu
authors.forename: Zhongmiao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Haibin
authors.initials: H
authors.lastname: Dai
mesh_terms: D002599:Chalcone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D011765:Pyruvaldehyde / Q000378:metabolism; D011809:Quinones / Q000494:pharmacology*; D012266:Ribose / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism
chemical_list: D017127:Glycation End Products, Advanced; D010455:Peptides; D011809:Quinones; C085278:hydroxysafflor yellow A; D012710:Serum Albumin, Bovine; D002599:Chalcone; D012266:Ribose; D011765:Pyruvaldehyde; D005947:Glucose; D008239:Lysine