title: Replacement of dynamic cultured biograft improves damaged heart function-comparative study of static cultured biografts-.
abstract: OBJECTIVE
  To determine whether a dynamic cultured biograft can positively affect the function of the damaged heart.
  
  
  METHODS
  We ligated the coronary artery (LAD) of rats to generate a model of myocardial infarction (MI) and then implanted them with the following grafts comprising vascular smooth muscle cells (VSMCs) derived from the rat aorta and seeded onto biodegradable patches (patch replacement therapy; (PRTx)): control without PRTx, PRTx without seeded cells, PRTx with static cultured VSMCs, PRTx with dynamic cultured VSMCs and sham-operated. Cultured VSMCs were labeled with PKH26 for identification after implantation, and the centre of the MI site was excised and replaced with an implanted biograft. Cardiac performance was monitored for 12 weeks thereafter and followed by a histological study.
  
  
  RESULTS
  Although the ejection fraction of the damaged heart improved in all groups that were transplanted with grafts, remodeling was prevented only in groups with a dynamic or static cultured patch. More cells were α-SMA-positive in the group with the dynamic, rather than the static cultured patch. Cells were positive for PKH26 in the biograft and in the infarcted myocardium.
  
  
  CONCLUSIONS
  Dynamic cultured biografts improved the function of the infarcted myocardium more than statically cultured biografts or those without cells.
authors.affiliation: Division of Cardiovascular Surgery, Department of Surgery, School of Medicine, Faculty of Medicine, Toho University, Tokyo, Japan. h.yokomuro@chic.ocn.ne.jp
authors.forename: Hiroki
authors.initials: H
authors.lastname: Yokomuro
authors.forename: Noritsugu
authors.initials: N
authors.lastname: Shiono
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Katayanagi
authors.forename: Masanori
authors.initials: M
authors.lastname: Hara
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yoshihara
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D058948:Cell Tracking; D002478:Cells, Cultured; D004195:Disease Models, Animal; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism / Q000637:transplantation*; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000637:transplantation*; D051381:Rats; D011917:Rats, Inbred Lew; D020127:Recovery of Function; D013318:Stroke Volume; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014463:Ultrasonography; D016277:Ventricular Function, Left; D020257:Ventricular Remodeling
chemical_list: D000199:Actins; D001672:Biocompatible Materials; D015415:Biomarkers; C498862:Acta2 protein, rat