title: [The effect of knockdown of transcription factor SCL/TAL-1 gene on the erythroid differentiation in EPO-induced K562 cell line].
abstract: OBJECTIVE
  To investigate the role of transcript factor SCL/TAL-1 gene in the erythroid differentiation through the knockdown of SCL/TAL-1 mRNA by RNA interference.
  
  
  METHODS
  The plasmid of pTRIP-dU3-RNAiTALh-EF1a-GFP with SCL/TAL1 shRNA was transfected into EPO-induced K562 cell line with erythroid differentiation via lentiviral vector system and the expression of SCL/TAL-1 mRNA decreased. The plasmid pTRIP-dU3- RNAiluc-EF1-GFP expressing EGFP gene was as control. The mRNA levels of SCL/TAL-1 and erythroid related RhD, GPA, CD47 in the cell lines were detected by RT-PCR, and erythroid antigen CD71, CD235a were examined by flow cytometry.
  
  
  RESULTS
  (1) After 48 h of transfect, more than 95% of K562 cells were GFP positive, indicating the infection rate of the plasmids in the K562 cells more than 95%. (2) The results of RT-PCR showed SCL/TAL-1 mRNA expression in the K562 cell line of knockdown of SCL/TAL-1 was significantly lower than that in the control (P < 0.05). The mRNA levels of CD47 and RhD were also significantly lower, however, GPA decreased slightly in comparison with the control. (3) The expressions of CD71 and CD235a markedly reduced in the K562 cell line of knockdown of SCL/TAL-1 with positive rates as 10.4% and 76.5%, while the positive rates in the control as 94.3% and 83.6%.
  
  
  CONCLUSIONS
  Our findings suggested that transcription factor SCL/TAL-1 might play an positive role in erythroid differentiation.
authors.affiliation: Department of Hematology and State Key Laboratory of Biotherapy, West China Hospital of Sichuan University, Chengdu 610041, China.
authors.forename: Rui-qing
authors.initials: RQ
authors.lastname: Zhou
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Gong
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.forename: Qing-qing
authors.initials: QQ
authors.lastname: Shan
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
mesh_terms: D002454:Cell Differentiation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D020014:K562 Cells; D034622:RNA Interference
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C071236:STIL protein, human