title: Proteomic identification of immunodominant chlamydial antigens in a mouse model.
abstract: Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen in the world. To identify new vaccine candidates a protein microarray was constructed by expressing the open reading frames (ORFs) from Chlamydia mouse pneumonitis (MoPn). C57BL/6, C3H/HeN and BALB/c mice were immunized either intranasally or intravaginally with live MoPn elementary bodies (EB). Two additional groups were immunized by the intramuscular plus subcutaneous routes with UV-treated EB, using CpG and Montanide as adjuvants to favor a Th1 response, or Alum, to elicit a Th2 response. Serum samples collected from the three strains of mice were tested in the microarray. The array included the expression of 909 proteins from the 921 ORFs of the MoPn genome and plasmid. A total of 530 ORFs were recognized by at least one serum sample. Of these, 36 reacted with sera from the three strains of mice immunized with live EB. These antigens included proteins that were previously described as immunogenic such as MOMP and HSP60. In addition, we uncovered new immunogens, including 11 hypothetical proteins. In summary, we have identified new immunodominant chlamydial proteins that can be tested for their ability to induce protection in animal models and subsequently in humans.
authors.affiliation: ImmPORT Therapeutics, Inc./Antigen Discovery Inc., 1 Technology Dr., Suite E309, Irvine, CA 92618, USA.
authors.forename: Andy
authors.initials: A
authors.lastname: Teng
authors.forename: Maria I
authors.initials: MI
authors.lastname: Cruz-Fisher
authors.forename: Chunmei
authors.initials: C
authors.lastname: Cheng
authors.forename: Sukumar
authors.initials: S
authors.lastname: Pal
authors.forename: Guifeng
authors.initials: G
authors.lastname: Sun
authors.forename: Pooja
authors.initials: P
authors.lastname: Ralli-Jain
authors.forename: Douglas M
authors.initials: DM
authors.lastname: Molina
authors.forename: Philip L
authors.initials: PL
authors.lastname: Felgner
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Liang
authors.forename: Luis M
authors.initials: LM
authors.lastname: de la Maza
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000276:immunology*; D002690:Chlamydia Infections / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D002692:Chlamydia trachomatis / Q000276:immunology* / Q000378:metabolism; D061387:Chlamydial Pneumonia / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D007114:Immunization; D016056:Immunodominant Epitopes / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D040081:Protein Array Analysis / Q000379:methods
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016056:Immunodominant Epitopes