title: Hepatoprotective effect of bis(4-methylbenzoyl) diselenide against CCl(4)-induced oxidative damage in mice.
abstract: From a pharmacological point of view, organoseleniums are compounds with important and interesting antioxidant and biological activities. The aim of this study was to evaluate the hepatoprotective effect of bis(4-methylbenzoyl) diselenide (BMD) against carbon tetrachloride (CCl4 )-induced oxidative damage in mice. The animals received BMD (25 mg/kg p.o., for 3 days), and after 1 day, CCl4 (1 mg/kg body weight) was administered by intraperitoneal route. One day after the CCl4 exposure, the animals were euthanized for biochemical and histological analysis. Treatment with BMD (25 mg/kg p.o.) protected against aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyl transferase and lactate dehydrogenase activity increases induced by CCl4 plasma exposure. Treatment with BMD (25 mg/kg) protected against increases in thiobarbituric reactive species and decreasing non-protein thiols and ascorbic acid levels in liver of mice. Catalase and superoxide dismutase activity inhibition in the liver caused by CCl4 were protected by treatment with BMD (25 mg/kg). Glutathione S-transferase activity was inhibited by CCl4 and remained unaltered even after treatment with BMD. Sections of liver from CCl4 -exposed mice presented an intense infiltration of inflammatory cells and loss of the cellular architecture. BMD (25 mg/kg) attenuated CCl4 -induced hepatic histological alterations. The results demonstrated the hepatoprotective effects of BMD in the mouse liver, possibly by modulating the antioxidant status.
authors.affiliation: Laboratório de Avaliações Farmacológicas e Toxicológicas Aplicadas às Moléculas Bioativas, LaftamBio Pampa, Universidade Federal do Pampa, CEP 97650-000, Itaqui, RS, Brazil.
authors.forename: Carlos Borges
authors.initials: CB
authors.lastname: Filho
authors.forename: Lucian
authors.initials: L
authors.lastname: Del Fabbro
authors.forename: Silvana P
authors.initials: SP
authors.lastname: Boeira
authors.forename: Ana Flávia
authors.initials: AF
authors.lastname: Furian
authors.forename: Lucielli
authors.initials: L
authors.lastname: Savegnago
authors.forename: Letiére Cabreira
authors.initials: LC
authors.lastname: Soares
authors.forename: Antonio Luiz
authors.initials: AL
authors.lastname: Braga
authors.forename: Cristiano R
authors.initials: CR
authors.lastname: Jesse
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008297:Male; D051379:Mice; D016566:Organoselenium Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D020011:Protective Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012643:Selenium / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000596:Amino Acids; D016566:Organoselenium Compounds; D020011:Protective Agents; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; C583859:bis(4-methylbenzoyl)diselenide; D002251:Carbon Tetrachloride; D012643:Selenium