title: Expression of FSH and its co-localization with FSH receptor and GnRH receptor in rat cerebellar cortex.
abstract: The expression of follicle-stimulating hormone (FSH) and its receptor in extrapituitary and non-HPG axis tissues has been demonstrated and their non-reproductive functions in these tissues have been found. However, there have been no reports concerning the expression and function of FSH and its receptor in the cerebellum. In our study, immunofluorescence staining and in situ hybridization were used to detect the expression of FSH, double-labeled immunofluorescence staining was used to detect co-localization of FSH and its receptor and co-localization of FSH and gonadotropin-releasing hormone (GnRH) receptor in the rat cerebellar cortex. Results showed that some cells of the Purkinje cell layer, granular layer, and molecular layer of the cerebellar cortex showed both FSH immunoreactivity and FSH mRNA positive signals; not only for FSH and FSH receptor, but also for FSH and GnRH receptor co-localized in some cells throughout the Purkinje cell layer, granular layer, and molecular layer of the cerebellar cortex. These suggested that rat cerebellum could express FSH; cerebellum is a target tissue of FSH; FSH may exert certain functions through FSH receptor in a paracrine or autocrine manner; GnRH may regulate FSH positive cells through GnRH receptor in the cerebellum. Our study provides morphological evidence for further functional research on FSH and related hormones in the cerebellum.
authors.affiliation: Department of Neurosurgery, Beijing, China. chuchenyu1977@yahoo.com.cn
authors.forename: Chenyu
authors.initials: C
authors.lastname: Chu
authors.forename: Jing'an
authors.initials: J
authors.lastname: Zhou
authors.forename: Yaqun
authors.initials: Y
authors.lastname: Zhao
authors.forename: Ce
authors.initials: C
authors.lastname: Liu
authors.forename: Pengfei
authors.initials: P
authors.lastname: Chang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Weiquan
authors.initials: W
authors.lastname: Huang
mesh_terms: D000818:Animals; D002525:Cerebellar Cortex / Q000378:metabolism*; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D011962:Receptors, FSH / Q000378:metabolism*; D011966:Receptors, LHRH / Q000378:metabolism*
chemical_list: D011962:Receptors, FSH; D011966:Receptors, LHRH; D005640:Follicle Stimulating Hormone