title: [Effect of perilipin 1 on chicken preadipocyte lipid accumulation].
abstract: OBJECTIVE
  To explore the effect of perilipin 1 on chicken preadipocyte lipid accumulation.
  
  
  METHODS
  Firstly, pcDNA3.1-perilipin 1 gene eukaryotic expression vector was constructed and transfected into primary cultured chicken preadipocytes. Twenty-four hours after transfection, chicken preadipocytes were cultured in complete medium with oleate, to induce preadipocyte differentiation. Then perilipin 1 was detected by Western blotting; the role of perilipin 1 in preadipocyte lipid accumulation was investigated by Oil Red O staining extraction assay; the expression levels of other genes (FAS, ACC and ATGL) related to the chicken adipocyte lipid metabolism were tested by real-time RT-PCR.
  
  
  RESULTS
  pcDNA3.1-perilipin 1 gene eukaryotic expression vector upregulated the expression of perilipin 1 in the transfected chicken preadipocytes. Over-expression of perilipin 1 promoted chicken preadipocyte lipid accumulation, while there was no significant change in the expression levels of other chicken lipid metabolism related genes (FAS, ACC and ATGL).
  
  
  CONCLUSIONS
  Over-expression of perilipin 1 can promote chicken preadipocyte lipid accumulation.
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China. zwn0501@163.com
authors.forename: Wei-nan
authors.initials: WN
authors.lastname: Zhou
authors.forename: Yu-xiang
authors.initials: YX
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D002645:Chickens; D050356:Lipid Metabolism / Q000187:drug effects*; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics / Q000502:physiology*; D013234:Stem Cells / Q000378:metabolism*; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D000070778:Perilipin-1; D010750:Phosphoproteins