title: Enzyme-linked immunosorbent assay of IL-8 production in response to silver nanoparticles.
abstract: Enzyme-linked immunosorbent assay (ELISA) for monitoring the effects of nanoparticles on immune cells is a conventional method of assessing the levels of cytokine that are released into the culture supernatant after the addition of nanoparticles to a macrophage culture. However, it has been found that the presence of nanoparticles can interfere with spectrophotometric analysis, used as an indicator test system; thus, it is necessary to thoroughly checked for the possibility of interference. In this chapter, the assessment method of cytokine production is covered in detail by utilizing the cytokine model produced by silver nanoparticles.
authors.affiliation: Department of Microbiology, College of Medicine, Yonsei University, Seoul, South Korea.
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Yang
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Jang
authors.forename: Dae-Hyoun
authors.initials: DH
authors.lastname: Lim
authors.forename: In-Hong
authors.initials: IH
authors.lastname: Choi
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D053768:Metal Nanoparticles / Q000633:toxicity*; D012015:Reference Standards; D012834:Silver / Q000633:toxicity*; D013048:Specimen Handling; D013053:Spectrophotometry; D020298:U937 Cells
chemical_list: D016209:Interleukin-8; D012834:Silver