title: HLA DR-DQ genotyping by capillary electrophoresis for risk assessment for celiac disease.
abstract: The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects.
authors.affiliation: Department of Biomedical Laboratory Science, Malmö University, Malmö, Sweden. Ewa.Lavant@skane.se
authors.forename: Ewa H
authors.initials: EH
authors.lastname: Lavant
authors.forename: Joyce
authors.initials: J
authors.lastname: Carlson
mesh_terms: D002446:Celiac Disease / Q000235:genetics* / Q000276:immunology*; D019075:Electrophoresis, Capillary / Q000379:methods*; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques / Q000379:methods*; D006683:HLA-DQ Antigens / Q000235:genetics* / Q000276:immunology; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D006720:Homozygote; D006801:Humans; D016133:Polymerase Chain Reaction; D018570:Risk Assessment; D013223:Statistics as Topic
chemical_list: D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens