title: Influence of the HPA axis on the inflammatory response in cutaneous wounds with the use of 670-nm laser photobiomodulation.
abstract: This study evaluated the influence of hypothalamic-pituitary-adrenal (HPA) axis in cutaneous wounds subjected to laser biomodulation. A total of 48 rats were divided into two groups: Group I (GI) with 24 adrenalectomized animals and Group II (GII) with 24 non-adrenalectomized animals. Each group was divided into two subgroups: the irradiated subgroup which laser was applied to four points at the edges of the wound (670 nm laser, 9 mW) and control subgroup. Rats in each subgroup were sacrificed at 24 or 72 h. Adrenal glands were only removed from GI rats. Three days after adrenalectomy, a cutaneous wound was made. An immunohistochemical analysis was performed using anti-CD45 and anti-CD8 antibodies. Flow cytometry was used to count T lymphocytes and their subpopulations in blood. Decreases in the number of CD45-positive inflammatory cells and in the total numbers of CD8- and CD45-positive cells were observed in histological sections of adrenalectomized animals subjected to laser biomodulation at 24h. Similar results were observed for distribution of total lymphocytes in blood (p<0.05). The action of 670 nm laser does not depend exclusively on HPA axis. It is believed that corticosteroid-promoting enzymes liberated in non-adrenal tissues may influence immune response under the influence of this type of phototherapy.
authors.affiliation: Escola Bahiana de Medicina e Saúde Pública - Dentistry Course, Av. Silveira Martins 3386, CEP 40150-100, Salvador, Bahia, Brazil. deylavilela@yahoo.com.br
authors.forename: Deyla Duarte Carneiro
authors.initials: DD
authors.lastname: Vilela
authors.forename: Flávia Villela
authors.initials: FV
authors.lastname: Chamusca
authors.forename: Jorge Clarêncio Souza
authors.initials: JC
authors.lastname: Andrade
authors.forename: Maria Lourdes Farre
authors.initials: ML
authors.lastname: Vallve
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Gonzalez
authors.forename: Zilton de Araújo
authors.initials: Zde A
authors.lastname: Andrade
authors.forename: Alena Ribeiro Alves Peixoto
authors.initials: AR
authors.lastname: Medrado
authors.forename: Sílvia Regina de Almeida
authors.initials: SR
authors.lastname: Reis
mesh_terms: D000311:Adrenal Glands / Q000503:physiopathology* / Q000601:surgery; D000315:Adrenalectomy / Q000009:adverse effects; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D007030:Hypothalamo-Hypophyseal System / Q000503:physiopathology*; D007249:Inflammation / Q000097:blood / Q000276:immunology / Q000503:physiopathology / Q000601:surgery; D053685:Laser Therapy; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000276:immunology / Q000293:injuries* / Q000503:physiopathology*