title: Bone marrow-derived cells can acquire renal stem cells properties and ameliorate ischemia-reperfusion induced acute renal injury.
abstract: BACKGROUND
  Bone marrow (BM) stem cells have been reported to contribute to tissue repair after kidney injury model. However, there is no direct evidence so far that BM cells can trans-differentiate into renal stem cells.
  
  
  METHODS
  To investigate whether BM stem cells contribute to repopulate the renal stem cell pool, we transplanted BM cells from transgenic mice, expressing enhanced green fluorescent protein (EGFP) into wild-type irradiated recipients. Following hematological reconstitution and ischemia-reperfusion (I/R), Sca-1 and c-Kit positive renal stem cells in kidney were evaluated by immunostaining and flow cytometry analysis. Moreover, granulocyte colony stimulating factor (G-CSF) was administrated to further explore if G-CSF can mobilize BM cells and enhance trans-differentiation efficiency of BM cells into renal stem cells.
  
  
  RESULTS
  BM-derived cells can contribute to the Sca-1(+) or c-Kit(+) renal progenitor cells population, although most renal stem cells came from indigenous cells. Furthermore, G-CSF administration nearly doubled the frequency of Sca-1+ BM-derived renal stem cells and increased capillary density of I/R injured kidneys.
  
  
  CONCLUSIONS
  These findings indicate that BM derived stem cells can give rise to cells that share properties of renal resident stem cell. Moreover, G-CSF mobilization can enhance this effect.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jia
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Xie
authors.forename: Guowei
authors.initials: G
authors.lastname: Feng
authors.forename: He
authors.initials: H
authors.lastname: Lű
authors.forename: Qinjun
authors.initials: Q
authors.lastname: Zhao
authors.forename: Yongzhe
authors.initials: Y
authors.lastname: Che
authors.forename: Yizhou
authors.initials: Y
authors.lastname: Zheng
authors.forename: Zhongchao
authors.initials: Z
authors.lastname: Han
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Zongjin
authors.initials: Z
authors.lastname: Li
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
mesh_terms: D058186:Acute Kidney Injury / Q000473:pathology* / Q000601:surgery*; D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D015427:Reperfusion Injury / Q000473:pathology* / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000473:pathology*; D016896:Treatment Outcome