title: Fc receptor-γ subunits with both polar or non-polar amino acids at position of T22 are capable of restoring surface expression of the high-affinity IgE receptor and degranulation in γ subunit-deficient rat basophilic leukemia cells.
abstract: The high-affinity IgE receptor (FcɛRI) is formed by the IgE-binding α subunit, β subunit and γ subunits homodimer. All three subunits are required for proper expression of the receptor on the plasma membrane of mast cells and basophils. However, the exact molecular mechanism of inter-subunit interactions required for correct expression and function of the FcɛRI complex remains to be identified. A recent study suggested that polar aspartate at position 194 within the transmembrane domain of the α subunit could interact by hydrogen bonding with polar threonine at position 22 in the transmembrane domains of the γ subunits. To verify this, we used previously isolated rat basophilic leukemia (RBL)-2H3 variant cells deficient in the expression of the FcɛRI-γ subunit (FcR-γ), and transfected them with DNA vectors coding for FcR-γ of the wild-type or mutants in which T22 was substituted for nonpolar alanine (T22A mutant) or polar serine (T22S mutant). Analysis of the transfectants showed that both T22A and T22S mutants were capable to restore surface expression of the FcɛRI similar to wild-type FcR-γ. Furthermore, cells transfected with wild-type, T22A or T22S FcR-γ showed comparably enhanced FcɛRI-mediated degranulation. Our data indicate that substitution of FcR-γ T22 with non-polar amino acid does not interfere with surface expression of the FcɛRI and its signaling capacity.
authors.affiliation: Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom.
authors.forename: Amir
authors.initials: A
authors.lastname: Rashid
authors.forename: Jonathan E M
authors.initials: JE
authors.lastname: Housden
authors.forename: Birgit A
authors.initials: BA
authors.lastname: Helm
authors.forename: Petr
authors.initials: P
authors.lastname: Draber
mesh_terms: D000409:Alanine / Q000737:chemistry; D019943:Amino Acid Substitution; D000818:Animals; D001491:Basophils / Q000276:immunology*; D015550:Cell Degranulation / Q000276:immunology; D045744:Cell Line, Tumor; D006860:Hydrogen Bonding; D015471:Leukemia, Basophilic, Acute / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D054730:Protein Interaction Domains and Motifs; D021122:Protein Subunits; D051381:Rats; D017455:Receptors, IgE / Q000737:chemistry* / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012694:Serine / Q000737:chemistry; D015398:Signal Transduction; D013912:Threonine / Q000737:chemistry; D014162:Transfection
chemical_list: C553474:FCER1A protein, rat; D050505:Mutant Proteins; D021122:Protein Subunits; D017455:Receptors, IgE; D011994:Recombinant Proteins; D013912:Threonine; D012694:Serine; D000409:Alanine