title: A label-free real time fluorometric assay for protease and inhibitor screening with a released heme.
abstract: A label free continuous assay for protease activity and inhibitor screening has been developed. A protease (trypsin) could digest hemoglobin. Free heme molecules were released. Strong π-π stacking and hydrophobic interactions with the perylene probe resulted in efficient quenching of the probe's monomer fluorescence.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Huping
authors.initials: H
authors.lastname: Jiao
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhang
authors.forename: Huipeng
authors.initials: H
authors.lastname: Zhou
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D000818:Animals; D002417:Cattle; D057075:Enzyme Assays / Q000379:methods; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D006801:Humans; D010569:Perylene / Q000032:analysis / Q000378:metabolism*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors / Q000494:pharmacology*
chemical_list: D005456:Fluorescent Dyes; D014361:Trypsin Inhibitors; D006418:Heme; D010569:Perylene; D014357:Trypsin