title: Intracellular Ca2+ and antioxidant values induced positive effect on fertilisation ratio and oocyte quality of granulosa cells in patients undergoing in vitro fertilisation.
abstract: Oxidative stress is important for promoting oocyte maturation and ovulation within the follicle through calcium ion (Ca(2+)) influx. The relationship between antioxidant and cytosolic Ca(2+) levels and oocyte quality and fertilisation rate in the granulosa cells of patients undergoing in vitro fertilisation was investigated. Granulosa cells were collected from 33 patients. Cytosolic free Ca(2+) ([Ca(2+)]i) concentration, lipid peroxidation, reduced glutathione, glutathione peroxidase and oocyte quality were measured in the granulosa cells. The relationship between two drug protocols was also examined (gonadotrophin-releasing hormone antagonist and agonist protocols) and the same parameters investigated. The [Ca(2+)]i concentration (P<0.001), glutathione (P<0.05) and oocyte quality (P<0.001) values were significantly higher in the fertilised group than in the non-fertilised group, although glutathione peroxidase activity was significantly (P<0.05) higher in the non-fertilised group than in the fertilised group. The [Ca(2+)]i concentrations were also higher (P<0.001) in the good-quality oocyte groups than in the poor-quality oocyte group. There was no correlation between the two drug protocols and investigated parameters. In conclusion, it was observed that high glutathione and cytosolic Ca(2+) concentrations in granulosa cells of patients undergoing in vitro fertilisation tended to increase the fertilisation potential of oocytes.
authors.affiliation: Department of Obstetrics and Gynaecology, Faculty of Medicine, Suleyman Demirel University, TR-32260, Isparta, Turkey.
authors.forename: Esra Nur
authors.initials: EN
authors.lastname: Tola
authors.forename: Muhittin Tamer
authors.initials: MT
authors.lastname: Mungan
authors.forename: Abdülhadi Cihangir
authors.initials: AC
authors.lastname: Uğuz
authors.forename: Mustafa
authors.initials: M
authors.lastname: Naziroğlu
mesh_terms: D000704:Analysis of Variance; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D020013:Calcium Signaling / Q000502:physiology*; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology*; D005307:Fertilization in Vitro / Q000379:methods*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006107:Granulosa Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D018709:Statistics, Nonparametric
chemical_list: D000975:Antioxidants; D005979:Glutathione Peroxidase; D005978:Glutathione; D002118:Calcium