title: Comparative proteomic analyses reveal the changes of metabolic features in soybean (Glycine max) pistils upon pollination.
abstract: Siphonogamy is a critical process in plant reproductive growth, during which numerous cell-cell interaction events occur between pistil and pollen. Previous studies in Solanaceae, Papaveraceae, and Brassicaceae focusing on pollen-stigma recognition in self-incompatible systems have provided many important views. In this study, we profiled the proteome in soybean mature pistils before and after pollination. Comparative analyses of two-dimensional gel electrophoresis maps from un-pollinated and pollinated pistils were conducted. The results showed that 22 proteins were increased and 36 proteins decreased after pollination. Functional categorization showed that most of them were metabolism- and redox-related proteins. The enhancement of primary metabolism, biosynthesis of pollen tube guidance compounds, and adjustment of redox homeostasis system might be helpful for a successful pollination. Quantitative reverse transcript-polymerase chain reaction analysis implied that the regulation of gene expression might happen at both transcriptional and posttranscriptional levels during pollination. This study will enhance our understanding of pollen-stigma interaction in plant sexual reproductive growth.
authors.affiliation: Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Aihua
authors.initials: A
authors.lastname: Sha
authors.forename: Xinan
authors.initials: X
authors.lastname: Zhou
authors.forename: Pingfang
authors.initials: P
authors.lastname: Yang
mesh_terms: D002450:Cell Communication; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D035264:Flowers / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D006706:Homeostasis; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011058:Pollen / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D054817:Pollination; D020543:Proteome; D040901:Proteomics; D018749:RNA, Plant / Q000235:genetics; D012098:Reproduction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013025:Glycine max / Q000166:cytology / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation
chemical_list: D010940:Plant Proteins; D020543:Proteome; D018749:RNA, Plant