title: Selenium has a protective effect on ischemia/reperfusion injury in a rat ovary model: biochemical and histopathologic evaluation.
abstract: OBJECTIVE
  The aim of the study was to evaluate the effects of selenium (Se) on ischemia/reperfusion (I/R) injury in rat ovaries.
  
  
  METHODS
  Thirty-five female Sprague-Dawley rats were randomly divided into 5 groups (n = 7): sham (S), I/R1, I/R2, Se1, and Se2. In the I/R1 and Se1 groups, 4 hours of ischemia was followed by 6 hours of reperfusion, and in the I/R2 and Se2 groups, 4 hours of ischemia was followed by 12 hours of reperfusion. In the Se groups, 30 minutes before reperfusion, a single dose of 0.2 mg/kg Se was administered intraperitoneally. The ovarian tissue levels of malondialdehyde (MDA) and nitric oxide (NO), and the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were measured biochemically. Tissue damage to ovarian tissue was scored by histopathologic examination.
  
  
  RESULTS
  The I/R groups had significantly higher MDA levels and lower CAT, SOD, and GPx activities than the sham group (P < .05). Although NO levels were significantly higher in the I/R1 group than in the sham group (P < .05), the NO levels in the I/R2 and sham groups were similar. Selenium pretreatment significantly lowered tissue MDA and NO levels and increased tissue SOD and GPx activities in the Se groups, compared with those in the I/R groups (P < .05). Catalase activities were significantly higher in the Se2 group than in the I/R2 group (P < .05). Catalase activities were higher in the Se1 group than in the I/R1 group, but the difference was not statistically significant. Treatment with Se significantly decreased the ovarian tissue damage scores in the Se2 group compared with those in the I/R2 group (P < .05).
  
  
  CONCLUSIONS
  Selenium is effective in preventing tissue damage induced by I/R in rat ovaries.
authors.affiliation: Kahramanmaras Sutcuimam University, Medical Faculty, Department of Emergency Medicine, Kahramanmaras, Turkey. selimbozkurt01@yahoo.com
authors.forename: Selim
authors.initials: S
authors.lastname: Bozkurt
authors.forename: Deniz Cemgil
authors.initials: DC
authors.lastname: Arikan
authors.forename: Ergul Belge
authors.initials: EB
authors.lastname: Kurutas
authors.forename: Hamide
authors.initials: H
authors.lastname: Sayar
authors.forename: Mehmet
authors.initials: M
authors.lastname: Okumus
authors.forename: Ayhan
authors.initials: A
authors.lastname: Coskun
authors.forename: Vedat
authors.initials: V
authors.lastname: Bakan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D005260:Female; D007274:Injections, Intraperitoneal; D007813:Laparotomy; D010049:Ovarian Diseases / Q000150:complications*; D010053:Ovary / Q000098:blood supply / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D012643:Selenium / Q000627:therapeutic use*; D014102:Torsion Abnormality / Q000150:complications*; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D012643:Selenium