title: Borna disease virus infection perturbs energy metabolites and amino acids in cultured human oligodendroglia cells.
abstract: BACKGROUND
  Borna disease virus is a neurotropic, non-cytolytic virus that has been widely employed in neuroscientific research. Previous studies have revealed that metabolic perturbations are associated with Borna disease viral infection. However, the pathophysiological mechanism underlying its mode of action remains unclear.
  
  
  METHODS
  Human oligodendroglia cells infected with the human strain Borna disease virus Hu-H1 and non-infected matched control cells were cultured in vitro. At day 14 post-infection, a proton nuclear magnetic resonance-based metabonomic approach was used to differentiate the metabonomic profiles of 28 independent intracellular samples from Borna disease virus-infected cells (n = 14) and matched control cells (n = 14). Partial least squares discriminant analysis was performed to demonstrate that the whole metabonomic patterns enabled discrimination between the two groups, and further statistical testing was applied to determine which individual metabolites displayed significant differences between the two groups.
  
  
  RESULTS
  Metabonomic profiling revealed perturbations in 23 metabolites, 19 of which were deemed individually significant: nine energy metabolites (α-glucose, acetate, choline, creatine, formate, myo-inositol, nicotinamide adenine dinucleotide, pyruvate, succinate) and ten amino acids (aspartate, glutamate, glutamine, glycine, histidine, isoleucine, phenylalanine, threonine, tyrosine, valine). Partial least squares discriminant analysis demonstrated that the whole metabolic patterns enabled statistical discrimination between the two groups.
  
  
  CONCLUSIONS
  Borna disease viral infection perturbs the metabonomic profiles of several metabolites in human oligodendroglia cells cultured in vitro. The findings suggest that Borna disease virus manipulates the host cell's metabolic network to support viral replication and proliferation.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Rongzhong
authors.initials: R
authors.lastname: Huang
authors.forename: Hongchang
authors.initials: H
authors.lastname: Gao
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Jianmin
authors.initials: J
authors.lastname: Jia
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Zheng
authors.forename: Lihua
authors.initials: L
authors.lastname: Ma
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Deng
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Mingju
authors.initials: M
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001890:Borna Disease / Q000378:metabolism*; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D009836:Oligodendroglia / Q000378:metabolism* / Q000821:virology
chemical_list: D000596:Amino Acids