title: Separation, purification and quantification of verbascoside from Penstemon barbatus (Cav.) Roth.
abstract: Echinacoside and verbascoside possess a spectrum of significant beneficial bioactivities. A new method of preparative high-speed counter-current chromatography for rapid separation and purification of verbascoside from the leaves of Penstemon barbatus (Cav.) Roth was introduced. The solvent system employed here was n-butanol-water (1:1, v/v). The structure was confirmed by IR, (1)H NMR and (13)C NMR. According to the quantification results, echinacoside and verbascoside were found to be as much as 5.25 ± 0.13 mg/g and 11.21 ± 0.16 mg/g, respectively, in the leaves of P. barbatus. This indicates that P. barbatus might be an ideal alternative resource for large scale preparation of echinacoside and verbascoside.
authors.affiliation: Department of Chemical Biology, School of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui Province 243000, China. xjljxchq@126.com
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
authors.forename: Feng
authors.initials: F
authors.lastname: Tan
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.forename: Caibo
authors.initials: C
authors.lastname: Yue
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D032263:Penstemon / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols
chemical_list: D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; C058956:acteoside; D059808:Polyphenols