title: The Role of M. leprae Hsp65 Protein and Peptides in the Pathogenesis of Uveitis.
abstract: Experimental autoimmune uveitis (EAU) is a well established model for immune-mediated organ-specific disease. Our group has recently shown that the M. leprae Hsp65 aggravated the uveitis in mice; in the present study, we evaluated the action of M. leprae  K(409)A mutant protein and the synthetic peptides Leader pep and K(409)A pep (covering amino acids residues 352-371 of WT and K(409)A proteins of M. leprae Hsp65, resp.) on the pathogenesis of EAU. Mice received the 161-180 IRBP peptide and B. pertussis toxin followed by the intraperitoneal inoculation of K(409)A protein or the Leader pep or K(409)A pep. The Leader pep aggravated the disease, but mice receiving the K(409)A pep did not develop the disease and presented an increase in IL-10 levels by spleen cells and a decrease in the percentage of CD4+ IFN-γ+ T cells. Moreover, animals receiving the Leader pep presented the highest scores of the disease associated with increase percentage of CD4+ IFN-γ+ T cells. These results would contribute to understanding of the pathogenesis of EAU and support the concept that immune responses to Hsp are of potential importance in exacerbating, perpetuating, or even controlling organ-restricted autoimmune diseases, and it is discussed the irreversibility of autoimmune syndromes.
authors.affiliation: Department of Ophthalmology, São Paulo Hospital, Federal University of São Paulo, R. Botucatu 820, 04023-062 São Paulo, SP, Brazil.
authors.forename: Alessandra Gonçalves
authors.initials: AG
authors.lastname: Commodaro
authors.forename: Eliana Blini
authors.initials: EB
authors.lastname: Marengo
authors.forename: Jean Pierre S
authors.initials: JP
authors.lastname: Peron
authors.forename: Wesley
authors.initials: W
authors.lastname: Brandao
authors.forename: Christina
authors.initials: C
authors.lastname: Arslanian
authors.forename: Robson Lopes
authors.initials: RL
authors.lastname: Melo
authors.forename: Estevam J
authors.initials: EJ
authors.lastname: Baldon
authors.forename: Rubens
authors.initials: R
authors.lastname: Belfort
authors.forename: Osvaldo Augusto
authors.initials: OA
authors.lastname: Sant'anna
authors.forename: Luiz Vicente
authors.initials: LV
authors.lastname: Rizzo