title: Stoichiometry of the human glycine receptor revealed by direct subunit counting.
abstract: The subunit stoichiometry of heteromeric glycine-gated channels determines fundamental properties of these key inhibitory neurotransmitter receptors; however, the ratio of α1- to β-subunits per receptor remains controversial. We used single-molecule imaging and stepwise photobleaching in Xenopus oocytes to directly determine the subunit stoichiometry of a glycine receptor to be 3α1:2β. This approach allowed us to determine the receptor stoichiometry in mixed populations consisting of both heteromeric and homomeric channels, additionally revealing the quantitative proportions for the two populations.
authors.affiliation: Institut de Ciències Fotòniques, 08860 Castelldefels (Barcelona), Spain, Institut universitaire en santé mentale de Québec, Québec, G1J 2G3 Canada.
authors.forename: Nela
authors.initials: N
authors.lastname: Durisic
authors.forename: Antoine G
authors.initials: AG
authors.lastname: Godin
authors.forename: Claudia M
authors.initials: CM
authors.lastname: Wever
authors.forename: Colin D
authors.initials: CD
authors.lastname: Heyes
authors.forename: Melike
authors.initials: M
authors.lastname: Lakadamyali
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Dent
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D009865:Oocytes / Q000737:chemistry* / Q000378:metabolism*; D021122:Protein Subunits / Q000032:analysis*; D018009:Receptors, Glycine / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D021122:Protein Subunits; D018009:Receptors, Glycine