title: Reduced expression of ELAVL4 in male meningioma patients.
abstract: Meningioma is a frequently occurring tumor of the central nervous system. Among many genetic alternations, the loss of the short arm of chromosome 1 is the second most frequent chromosomal abnormality observed in these tumors. Here, we focused on the previously described and well-established minimal deletion regions of chromosome 1. In accordance with the Knudson suppressor theory, we designed an analysis of putative suppressor genes localized in the described minimal deletion regions. The purpose was to determine the molecular background of the gender-specific occurrence of meningiomas. A total of 149 samples were examined for loss of heterozygosity (LOH). In addition, 57 tumor samples were analyzed using real-time polymerase chain reaction. We examined the association between the expression of selected genes and patient age, gender, tumor grade and presence of 1p loss. Furthermore, we performed an analysis of the most stable internal control for real-time analysis in meningiomas. LOH analysis revealed gender-specific discrepancies in the frequency of 1p aberrations. Moreover, statistical correlation between the gene expression level and gender was significant for the ELAVL4 gene as we found it to be lower in males than in females. We conclude that meningiomas present different features depending on patient gender. We suggest that ELAVL4 can be involved in the pathogenesis of meningiomas in male patients.
authors.affiliation: Department of Molecular Pathology and Neuropathology, Medical University of Lodz, Czechoslowacka 8/10, 92-216 Lodz, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Stawski
authors.forename: Sylwester
authors.initials: S
authors.lastname: Piaskowski
authors.forename: Ewelina
authors.initials: E
authors.lastname: Stoczynska-Fidelus
authors.forename: Krystyna
authors.initials: K
authors.lastname: Wozniak
authors.forename: Michal
authors.initials: M
authors.lastname: Bienkowski
authors.forename: Magdalena
authors.initials: M
authors.lastname: Zakrzewska
authors.forename: Monika
authors.initials: M
authors.lastname: Witusik-Perkowska
authors.forename: Dariusz J
authors.initials: DJ
authors.lastname: Jaskolski
authors.forename: Waldemar
authors.initials: W
authors.lastname: Och
authors.forename: Wielislaw
authors.initials: W
authors.lastname: Papierz
authors.forename: Beata
authors.initials: B
authors.lastname: Sikorska
authors.forename: Piotr
authors.initials: P
authors.lastname: Rieske
authors.forename: Pawel P
authors.initials: PP
authors.lastname: Liberski
mesh_terms: D000328:Adult; D000368:Aged; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D051959:ELAV Proteins / Q000235:genetics*; D000067779:ELAV-Like Protein 4; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D008875:Middle Aged; D012727:Sex Characteristics
chemical_list: D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; C498010:ELAVL4 protein, human