title: Gelatin hydrolysate from blacktip shark skin prepared using papaya latex enzyme: Antioxidant activity and its potential in model systems.
abstract: Antioxidant activities of gelatin hydrolysates from blacktip shark skin prepared using papaya latex enzyme with different degrees of hydrolysis (DHs: 10%, 20%, 30% and 40%) were evaluated. All antioxidant activity indices of hydrolysates increased with increasing DH (P<0.05). When gelatin hydrolysate with 40%DH was determined for its pH and thermal stability, ORAC and chelating activity remained constant or slightly increased in a wide pH range (1-9) and during heating (100°C) for 240min. It was also stable in simulated gastrointestinal tract model system. Moreover, gelatin hydrolysate at a level of 500 and 1000ppm could inhibit lipid oxidation in both β-carotene linoleate and cooked comminuted pork model systems. Therefore, gelatin hydrolysate from blacktip shark skin (40%DH) can potentially be used as an alternative source of natural antioxidants.
authors.affiliation: Department of Food Technology, Faculty of Agro-Industry Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Phanat
authors.initials: P
authors.lastname: Kittiphattanabawon
authors.forename: Soottawat
authors.initials: S
authors.lastname: Benjakul
authors.forename: Wonnop
authors.initials: W
authors.lastname: Visessanguan
authors.forename: Fereidoon
authors.initials: F
authors.lastname: Shahidi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D029441:Carica / Q000737:chemistry / Q000201:enzymology*; D029941:Fish Proteins / Q000737:chemistry* / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D006358:Hot Temperature; D006868:Hydrolysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008954:Models, Biological; D010940:Plant Proteins / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000494:pharmacology; D055550:Protein Stability; D012867:Skin / Q000737:chemistry*; D019207:beta Carotene / Q000378:metabolism
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D010940:Plant Proteins; D011492:Protein Hydrolysates; D019207:beta Carotene; D005780:Gelatin