title: Hydrolysis of surimi wastewater for production of transglutaminase by Enterobacter sp. C2361 and Providencia sp. C1112.
abstract: Surimi wastewater (SWW) is an industrial wastewater, released during the washing step of surimi preparation from minced fish, that causes environmental problem. In this study, SWW produced from ornate threadfin bream (Nemipterus hexodon) was hydrolysed and used to cultivate Enterobacter sp. C2361 and Providencia sp. C1112 for the production of microbial transglutaminase (MTGase, EC 2.3.2.13). The SWW was repeatedly used to wash the fish mince that gained a final protein content of 3.20% (w/v). The commercial protease, Delvolase was the most appropriate protease used to produce fish protein hydrolysate (FPH) from SWW. The FPH at 40% degree of hydrolysis was used instead of a peptone portion in the SPY medium (3.0% starch, 2.0% peptone, 0.2% yeast extract, 0.2% MgSO(4), 0.2% K(2)HPO(4) and 0.2% KH(2)HPO(4), pH 7.0) to cultivate the tested strains at 37°C, shaking speed at 150rpm. Providencia sp. C1112 produced higher MTGase activity (1.78±0.05U/ml) than Streptoverticillium mobaraense (1.61±0.02U/ml) at 18h of cultivation in FPH medium. On the other hand, the Enterobacter sp. C2361 produced lower MTGase activity (1.18±0.03U/ml).
authors.affiliation: Department of Industrial Biotechnology, Prince of Songkla University, Hat Yai 90112, Thailand. aran.h@psu.ac.th
authors.forename: Aran
authors.initials: A
authors.lastname: H-Kittikun
authors.forename: Chaiwut
authors.initials: C
authors.lastname: Bourneow
authors.forename: Soottawat
authors.initials: S
authors.lastname: Benjakul
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D001673:Biodegradation, Environmental; D004754:Enterobacter / Q000201:enzymology* / Q000378:metabolism; D029941:Fish Proteins / Q000737:chemistry / Q000378:metabolism; D005399:Fishes; D006868:Hydrolysis; D007220:Industrial Waste / Q000032:analysis; D011492:Protein Hydrolysates / Q000737:chemistry / Q000378:metabolism; D011532:Providencia / Q000201:enzymology* / Q000378:metabolism*; D011503:Transglutaminases / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D029941:Fish Proteins; D007220:Industrial Waste; D011492:Protein Hydrolysates; D011503:Transglutaminases; D062065:Wastewater