title: Molecular beacon-based quantitiation of epithelial tumor marker mucin 1.
abstract: Mucin 1 (Muc1) is a glycoprotein expressed on most epithelial cell surfaces, which has been confirmed as a useful biomarker for the diagnosis of early cancers. In this study, we demonstrate that a quantum dot (QD)-aptamer beacon acts by folding-induced dissociation of a DNA intercalating dye, BOBO-3, in the presence of the target molecules, Muc1. Release of intercalated BOBO-3s from the QD-conjugated aptamers results in a decrease in QD fluorescence resonance energy transfer (FRET)-mediated BOBO-3 emission, allowing for label-free Muc1 detection and quantitation. We attain highly specific and wide-range detection (from 50nM to 20μM) of Muc1, suggesting that our QD-aptamer beacon can be a potential alternative to immuno-based assays for Muc1 detection. The detection methodology is expected to be improved for the early diagnosis of different types of epithelial cancers of large populations.
authors.affiliation: Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, South Korea.
authors.forename: Seonmi
authors.initials: S
authors.lastname: Shin
authors.forename: Hye Yeon
authors.initials: HY
authors.lastname: Nam
authors.forename: Eun Jeong
authors.initials: EJ
authors.lastname: Lee
authors.forename: Woong
authors.initials: W
authors.lastname: Jung
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Hah
mesh_terms: D052157:Aptamers, Nucleotide / Q000032:analysis / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis*; D002232:Carbocyanines / Q000032:analysis / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D015335:Molecular Probes / Q000032:analysis* / Q000737:chemistry; D018396:Mucin-1 / Q000032:analysis*; D009375:Neoplasms, Glandular and Epithelial / Q000737:chemistry* / Q000175:diagnosis; D045663:Quantum Dots
chemical_list: D052157:Aptamers, Nucleotide; C098829:BOBO 3; D014408:Biomarkers, Tumor; D002232:Carbocyanines; D015335:Molecular Probes; D018396:Mucin-1