title: New method for effectively and quantitatively labeling cysteine residues on chicken eggshell membrane.
abstract: Using maleimidoethylmonoamide cysteine (Fmoc)(StBu) (1) as a medium, cysteine residues on proteins of chicken eggshell membrane (ESM) were successfully converted into N-terminal cysteines. After a biocompatible condensation reaction between the N-terminal cysteine and fluorescent probe 2-cyanobenzothiazole-Gly-Gly-Gly-fluorescein isothiocyanate (2), a new fluorogenic structure luciferin-Gly-Gly-Gly-FITC (3) was obtained, which exhibits a 2-fold fluorescence emission increase compared to that of 2. Thus, a new method for effectively labeling cysteine residues on ESMs was developed. Enhanced fluorescence images of ESMs were directly observed under a microscope and a small animal imaging machine.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.forename: Bin
authors.initials: B
authors.lastname: Mei
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Tian
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Chunyan
authors.initials: C
authors.lastname: Cao
authors.forename: Guangming
authors.initials: G
authors.lastname: Lu
authors.forename: Gaolin
authors.initials: G
authors.lastname: Liang
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000032:analysis*; D004527:Egg Proteins / Q000737:chemistry; D004528:Egg Shell / Q000737:chemistry*; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004527:Egg Proteins; D005456:Fluorescent Dyes; D008565:Membrane Proteins; D003545:Cysteine