title: Comparison of IRMS and NMR spectrometry for the determination of intramolecular 13C isotope composition: application to ethanol.
abstract: Isotopic (13)C NMR is a relatively recent technique which allows the determination of intramolecular (13)C isotope composition at natural abundance. It has been used in various scientific fields such as authentication, counterfeiting or plant metabolism. Although its precision has already been evaluated, the determination of its trueness remains still challenging. To deal with that issue, a comparison with another normalized technique must be achieved. In this work, we compare the intramolecular (13)C isotope distribution of ethanol from different origins obtained using both Isotope Ratio Mass Spectrometry (IRMS) and Nuclear Magnetic Resonance (NMR) spectrometry techniques. The IRMS approach consists of the oxidation of ethanol to acetic acid followed by the degradation of the latter for the analysis of each fragments formed. We show here that the oxidation of ethanol to acetic acid does not bring any significant error on the determination of the site-specific δ(13)C (δ(13)C(i)) of ethanol using the IRMS approach. The difference between the data obtained for 16 samples from different origins using IRMS and NMR approaches is not statistically significant and remains below 0.3‰. These results are encouraging for the future studies using isotopic NMR, especially in combination with the IRMS approach.
authors.affiliation: Department of Environmental Chemistry and Engineering, Tokyo Institute of Technology, Yokohama, Kanagawa 226-8503, Japan. gilbert.a.aa@m.titech.ac.jp
authors.forename: Alexis
authors.initials: A
authors.lastname: Gilbert
authors.forename: Ryota
authors.initials: R
authors.lastname: Hattori
authors.forename: Virginie
authors.initials: V
authors.lastname: Silvestre
authors.forename: Nariaki
authors.initials: N
authors.lastname: Wasano
authors.forename: Serge
authors.initials: S
authors.lastname: Akoka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirano
authors.forename: Keita
authors.initials: K
authors.lastname: Yamada
authors.forename: Naohiro
authors.initials: N
authors.lastname: Yoshida
authors.forename: Gérald S
authors.initials: GS
authors.lastname: Remaud