title: Endothelium-dependent vasodilator and antioxidant properties of a novel enzymatic extract of grape pomace from wine industrial waste.
abstract: The aim of the present study was to evaluate the vascular effects of an enzymatic extract of grape pomace (GP-EE) on isolated arteries, focusing our attention on endothelium-derived relaxation and on its antioxidant properties. Grape pomace derived from wine making was extracted by an enzymatic process and its composition of polyphenols was evaluated by HPLC and ESI-MS/MS, detecting kaempferol, catechin, quercetin and procyanidins B1 and B2, trace levels of resveratrol and tracing out gallocatechin and anthocyanidins. GP-EE induced endothelium- and NO-dependent vasodilatation of both rat aorta and small mesenteric artery (SMA) segments and reduced Phe-induced response in aortic rings. Both ORAC and DPPH assays confirmed antioxidant scavenging properties of GP-EE, which also prevented O(2)(·-) production (assessed by DHE fluorescence) and contraction elicited by ET-1. These results provide evidence that GP-EE possesses interesting antioxidant and protective vascular properties and highlight the potential interest of this extract as a functional food.
authors.affiliation: Departamento de Farmacología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain.
authors.forename: Rosalia
authors.initials: R
authors.lastname: Rodriguez-Rodriguez
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Justo
authors.forename: Carmen Maria
authors.initials: CM
authors.lastname: Claro
authors.forename: Elisabet
authors.initials: E
authors.lastname: Vila
authors.forename: Juan
authors.initials: J
authors.lastname: Parrado
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Herrera
authors.forename: Maria
authors.initials: M
authors.lastname: Alvarez de Sotomayor
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D045462:Endothelium-Dependent Relaxing Factors / Q000737:chemistry / Q000494:pharmacology*; D066298:In Vitro Techniques; D007220:Industrial Waste / Q000032:analysis*; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014664:Vasodilation / Q000187:drug effects; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D000975:Antioxidants; D045462:Endothelium-Dependent Relaxing Factors; D007220:Industrial Waste; D010936:Plant Extracts