title: Single nucleotide polymorphisms in the bovine genome are associated with the number of oocytes collected during ovum pick up.
abstract: The number of follicles recruited in each estrous cycle has gained practical importance in artificial reproductive technology, as it determines the oocyte yield from ultrasound-guided ovum pickup for in vitro embryo production. We aimed to identify single nucleotide polymorphisms (SNPs) in bovine genes related to reproductive physiology and evaluate the association between the candidate SNPs and the number of oocytes collected from ultrasound-guided ovum pickup. We sequenced genomic segments of GDF9, FGF8, FGF10 and BMPR2 and identified seventeen SNPs in the Bos taurus and Bos indicus breeds. Two SNPs cause amino acid changes in the proteins GDF9 and FGF8. Three SNPs in GDF9, FGF8 and BMPR2 were genotyped in 217 Nelore cows (B. indicus), while two previously identified mutations in LHCGR and mitochondrial DNA (mtDNA) were genotyped in the same group. The polymorphisms in GDF9, FGF8, BMRP2 and LHCGR were significantly associated (P<0.01) with the number of oocytes collected by ovum pickup, whereas the SNP in the mtDNA was not. In addition, we estimated an allelic substitution effect of 1.13±0.01 (P<0.01) oocytes for the SNP in the FGF8 gene. The results we report herein provide further evidence to support the hypothesis that genetic variability is an important component of the number of antral follicles in the bovine ovary.
authors.affiliation: Departamento de Ciências Básicas, FZEA-Universidade de São Paulo, Brazil.
authors.forename: W K F
authors.initials: WK
authors.lastname: Santos-Biase
authors.forename: F H
authors.initials: FH
authors.lastname: Biase
authors.forename: J
authors.initials: J
authors.lastname: Buratini
authors.forename: J
authors.initials: J
authors.lastname: Balieiro
authors.forename: Y F
authors.initials: YF
authors.lastname: Watanabe
authors.forename: M F
authors.initials: MF
authors.lastname: Accorsi
authors.forename: C R
authors.initials: CR
authors.lastname: Ferreira
authors.forename: P
authors.initials: P
authors.lastname: Stranieri
authors.forename: A R
authors.initials: AR
authors.lastname: Caetano
authors.forename: F V
authors.initials: FV
authors.lastname: Meirelles
mesh_terms: D000818:Animals; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000235:genetics / Q000378:metabolism; D002417:Cattle / Q000235:genetics / Q000502:physiology; D002452:Cell Count; D004526:Efficiency / Q000502:physiology; D005260:Female; D051526:Fibroblast Growth Factor 10 / Q000235:genetics / Q000378:metabolism; D051524:Fibroblast Growth Factor 8 / Q000235:genetics / Q000378:metabolism; D056726:Genetic Association Studies; D016678:Genome / Q000235:genetics; D055429:Growth Differentiation Factor 9 / Q000235:genetics / Q000378:metabolism; D054315:Oocyte Retrieval / Q000662:veterinary; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism / Q000502:physiology; D020641:Polymorphism, Single Nucleotide / Q000502:physiology*
chemical_list: D051526:Fibroblast Growth Factor 10; D055429:Growth Differentiation Factor 9; D051524:Fibroblast Growth Factor 8; D052006:Bone Morphogenetic Protein Receptors, Type II