title: The study of ghrelin deacylation enzymes.
abstract: Like other posttranslational modifications, fatty acid modification of amino acid residues in peptide chains is a critical determinant of their functional properties. A unique feature of ghrelin is the attachment of an acyl moiety at the third serine residue. Ghrelin is a hormone present in the circulation with roles in the release of growth hormone, control of behaviors related to appetite, and diverse cellular functions. Although lipid modification of ghrelin is essential for its binding to the ghrelin receptor, several lines of evidence suggest that deacylated ghrelin has physiological activity or activities similar to and distinct from the activities of the acylated form. Therefore, the understanding of deacylating process of ghrelin in vivo is key to accepting the physiological importance of ghrelin. In this review, we summarize results and methodology relevant to our recent efforts to determine the molecular mechanisms involved in ghrelin processing, including (1) immunological and mass spectrometry-based detection of ghrelin, (2) quantification of ghrelin deacylase activity, and (3) characterization of ghrelin deacylation enzymes isolated from biological fluids and using heterologous expression systems.
authors.affiliation: Department of Biochemistry, Dokkyo Medical University School of Medicine, Mibu, Tochigi, Japan.
authors.forename: Motoyasu
authors.initials: M
authors.lastname: Satou
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sugimoto
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000737:chemistry; D000215:Acylation; D000818:Animals; D002802:Cholinesterases / Q000737:chemistry* / Q000235:genetics; D017077:Culture Media, Conditioned / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods*; D015971:Gene Expression Regulation, Enzymologic; D054439:Ghrelin / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013869:Thiolester Hydrolases / Q000737:chemistry
chemical_list: D017077:Culture Media, Conditioned; D018076:DNA, Complementary; D054439:Ghrelin; D011994:Recombinant Proteins; C422650:ghrelin, des-n-octanoyl; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D002802:Cholinesterases; C402226:LYPLA1 protein, human; D013869:Thiolester Hydrolases