title: Sulfation modification and anticoagulant activity of the polysaccharides obtained from persimmon (Diospyros kaki L.) fruits.
abstract: The optimal conditions for sulfation of polysaccharides from persimmon fruits (PFP) with chlorosulfonic acid-pyridine (CSA-Pyr) method were determined by response surface methodology. Box-Behnken design was applied to evaluate the effects of three independent variables (volume ratio of Pyr to CSA, volume ratio of PFP to SO(3)Pyr and reaction time) on the degree of substitution (DS), molecular weight (MW) and activated partial thromboplastin time (APTT) of sulfated polysaccharides (PFP-S). The APTT activity of PFP-S could be improved by application of various volume ratio of Pyr to CSA, volume ratio of PFP to SO(3)Pyr and reaction time, which was possible due to the degradation of polysaccharides to different extent and increasing of DS. The optimal conditions to obtain the strongest APTT of PFP-S were the volume ratio of CSA to Pyr of 1:8, the volume ratio of SO(3)Pyr to PFP of 1:3.6 and the reaction time of 3 h, respectively.
authors.affiliation: Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Lu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Mo
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D029788:Diospyros / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D015233:Models, Statistical; D008970:Molecular Weight; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D011725:Pyridines / Q000737:chemistry; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D011725:Pyridines; D013431:Sulfates; D013451:Sulfonic Acids; C013880:chlorosulfonic acid; C023666:pyridine