title: 2'-OH of mRNA are critical for the binding of its codons at the 40S ribosomal P site but not at the mRNA entry site.
abstract: The roles of 2'-OH groups in the binding of mRNA to human ribosomes were studied using site-directed cross-linking. We found that both mRNA and mDNA analogues bearing a cross-linker can modify ribosomal proteins (rps) S3e and S2e at the mRNA entry site independently on tRNA presence, but only mRNA analogues were capable of a tRNA(Phe)-dependent binding to human ribosomes and cross-linking to rpS26e in the mRNA binding centre. Thus, 2'-OH groups of mRNA are unimportant for binding at the entry site but they are crucial for codon-anticodon interactions at the P site, implying the existence of mRNA-ribosome contacts that do not occur in bacteria.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia.
authors.forename: Dmitri
authors.initials: D
authors.lastname: Graifer
authors.forename: Andrey
authors.initials: A
authors.lastname: Zhigailov
authors.forename: Aliya
authors.initials: A
authors.lastname: Ven'yaminova
authors.forename: Alexey
authors.initials: A
authors.lastname: Malygin
authors.forename: Bulat
authors.initials: B
authors.lastname: Iskakov
authors.forename: Galina
authors.initials: G
authors.lastname: Karpova
mesh_terms: D001386:Azides / Q000737:chemistry; D001483:Base Sequence; D001665:Binding Sites; D003062:Codon / Q000378:metabolism*; D006801:Humans; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism; D054682:Ribosome Subunits, Small, Eukaryotic / Q000235:genetics / Q000378:metabolism*
chemical_list: D001386:Azides; D003062:Codon; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D012343:RNA, Transfer