title: Adipose tissue deletion of Gpr116 impairs insulin sensitivity through modulation of adipose function.
abstract: G protein-coupled receptor 116 (GPR116) is a novel member of the G protein-coupled receptors and its function is largely unknown. To investigate the physiological function of GPR116 in vivo, we generated adipose tissue specific conditional Gpr116 knockout mice (CKO) and fed them on standard chow or high fat diets. Selective deletion of Gpr116 in adipose tissue caused a pronounced glucose intolerance and insulin resistance in mice, especially when challenged with a high fat diet. Biochemical analysis revealed a more severe hepatosteatosis in CKO mice. Additionally, we found that CKO mice showed a lowered concentration of circulating adiponectin and an increased level of serum resistin. Our study suggests that GPR116 may play a critical role in controlling adipocyte biology and systemic energy homeostasis.
authors.affiliation: Key Laboratory of Regenerative Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Nie
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Hui
authors.forename: Xuefei
authors.initials: X
authors.lastname: Gao
authors.forename: Kuai
authors.initials: K
authors.lastname: Li
authors.forename: Wanhua
authors.initials: W
authors.lastname: Lin
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Xiang
authors.forename: Mengxiao
authors.initials: M
authors.lastname: Ding
authors.forename: Ying
authors.initials: Y
authors.lastname: Kuang
authors.forename: Aimin
authors.initials: A
authors.lastname: Xu
authors.forename: Jian
authors.initials: J
authors.lastname: Fei
authors.forename: Zhugang
authors.initials: Z
authors.lastname: Wang
authors.forename: Donghai
authors.initials: D
authors.lastname: Wu
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism / Q000473:pathology; D054392:Adipokines / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism* / Q000473:pathology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D048429:Cell Size; D005786:Gene Expression Regulation / Q000235:genetics; D055786:Gene Knockout Techniques; D018149:Glucose Intolerance / Q000235:genetics; D006949:Hyperlipidemias / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D007333:Insulin Resistance / Q000235:genetics*; D050356:Lipid Metabolism / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D043562:Receptors, G-Protein-Coupled / Q000172:deficiency* / Q000235:genetics*
chemical_list: D054392:Adipokines; C577038:Gpr116 protein, mouse; D043562:Receptors, G-Protein-Coupled