title: [Induction of anti-hepatocellular carcinoma immunity by Hyper-IL-6 gene in vivo].
abstract: OBJECTIVE
  To prepare the mouse hepatocellular carcinoma (HCC) cells with the stable expression of Hyper-IL-6 and explore the possibility of inducing active anti-HCC immune response by Hyper-IL-6 gene.
  
  
  METHODS
  Hyper-IL-6 gene was transfected into mouse HCC cells MM45T.Li using Lipofectamine(TM);2000. G418-resistant clones named MM45T-HIL-6 were selected and detected for the expression of Hyper-IL-6 gene by RT-PCR and ELISA. Mouse HCC cells transfected with pEGFP-C1,  named MM45T-mock,  were prepared as controls. Tumor models were established by injecting subcutaneously 5×10(5); cells of MM45T.Li,  MM45T- mock and MM45T-HIL-6 on the right anterior limb of BALB/c mouse,  respectively. In vivo experiments were performed to observe the tumorigenicity of MM45T.Li,  MM45T-mock and MM45T-HIL-6. The levels of CD4(+); and CD8(+); T cells in mouse peripheral blood were detected by flow cytometry (FCM).
  
  
  RESULTS
  RT-PCR and ELISA showed that Hyper-IL-6 gene was expressed in the MM45T-HIL-6 cells,  but not in the control cells. We observed that the tumorigenicity of MM45T-HIL-6 decreased compared with control cells after they were inoculated subcutaneously into mice. FCM results indicated that the levels of CD4(+); and CD8(+); T cells in the peripheral blood significantly increased in the mice inoculated with MM45T-HIL-6 compared with the ones inoculated with MM45T.Li and MM45T-mock cells (P<0.05).
  
  
  CONCLUSIONS
  The mouse HCC cells with the stable expression of Hyper-IL-6 can induce active anti-HCC immune response after inoculated subcutaneously into mice.
authors.affiliation: Tianjin Medical Research Institute of Liver Disease, Tianjin, China. qiech@126.com
authors.forename: Chun-hua
authors.initials: CH
authors.lastname: Qie
authors.forename: Shun-tian
authors.initials: ST
authors.lastname: Li
authors.forename: Wu-kui
authors.initials: WK
authors.lastname: Cao
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Shu-ren
authors.initials: SR
authors.lastname: Liang
mesh_terms: D000818:Animals; D005260:Female; D015316:Genetic Therapy; D015850:Interleukin-6 / Q000235:genetics*; D008114:Liver Neoplasms, Experimental / Q000276:immunology* / Q000628:therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection
chemical_list: D015850:Interleukin-6