title: Use of native gels to measure protein binding to SSB.
abstract: We describe a procedure to detect protein binding to SSB by polyacrylamide gel electrophoresis under non-denaturing conditions. As an example, we show the interaction of Thermus thermophilus (Tth) SSB with its cognate RecO protein. The interaction is detected as decay of the band corresponding to SSB by addition of RecO. We also demonstrate analysis of the RecO-RecR interaction as another example of this method.
authors.affiliation: Cellular & Molecular Biology Unit, RIKEN Advanced Science Institute, Yokohama, Japan.
authors.forename: Jin
authors.initials: J
authors.lastname: Inoue
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Mikawa
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008967:Molecular Biology / Q000379:methods; D011485:Protein Binding; D011995:Recombination, Genetic; D016963:Thermus thermophilus / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins