title: Fabrication of a glucose biosensor based on citric acid assisted cobalt ferrite magnetic nanoparticles.
abstract: A novel and practical glucose biosensor was fabricated with immobilization of Glucose oxidase (GOx) enzyme on the surface of citric acid (CA) assisted cobalt ferrite (CF) magnetic nanoparticles (MNPs). This innovative sensor was constructed with glassy carbon electrode which is represented as (GOx)/CA-CF/(GCE). An explicit high negative zeta potential value (-22.4 mV at pH 7.0) was observed on the surface of CA-CF MNPs. Our sensor works on the principle of detection of H2O2 which is produced by the enzymatic oxidation of glucose to gluconic acid. This sensor has tremendous potential for application in glucose biosensing due to the higher sensitivity 2.5 microA/cm2-mM and substantial increment of the anodic peak current from 0.2 microA to 10.5 microA.
authors.affiliation: Department of Metallurgical Engineering and Materials Science, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Krishna
authors.forename: Elby
authors.initials: E
authors.lastname: Titus
authors.forename: Sudeshna
authors.initials: S
authors.lastname: Chandra
authors.forename: Neel Kanth
authors.initials: NK
authors.lastname: Bardhan
authors.forename: Rohit
authors.initials: R
authors.lastname: Krishna
authors.forename: Dhirendra
authors.initials: D
authors.lastname: Bahadur
authors.forename: José
authors.initials: J
authors.lastname: Gracio
mesh_terms: D015374:Biosensing Techniques; D019343:Citric Acid / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D008280:Magnetics; D053768:Metal Nanoparticles; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission
chemical_list: D005290:Ferric Compounds; C001215:ferrite; D019343:Citric Acid; D003035:Cobalt; D005947:Glucose