title: Imidazo-benzothiazoles a potent microRNA modulator involved in cell proliferation.
abstract: MicroRNAs are endogenously expressed tiny non-coding RNAs that control gene expression at the post-transcriptional level and regulate processes of cell growth, differentiation, proliferation and apoptosis. Aberrant expression of microRNAs correlates with various cancers. Our experiments demonstrated that imidazo-benzothiazole conjugates caused apoptosis in colon cancer cells by modulating the expression of microRNAs. In vivo study in Drosophila melanogaster has exhibited inhibitory action on bantam microRNA, the homolog of human miR-542-5p that is involved in deciding the cellular cues that regulate the balance between proliferation and apoptosis. The expression of direct targets of bantam such as Hid and HDAC-6 were affected upon compound treatment. Interestingly, these conjugates downregulate the genes involved in microRNA biogenesis such as Drosha, Pasha and Dicer-1. Our findings have elucidated the microRNA inhibitory role of imidazo-benzothiazole conjugates.
authors.affiliation: Centre for Chemical Biology, Indian Institute of Chemical Technology, Hyderabad 500607, India.
authors.forename: Sreerangam N C V L
authors.initials: SN
authors.lastname: Pushpavalli
authors.forename: M Janaki
authors.initials: MJ
authors.lastname: Ramaiah
authors.forename: A
authors.initials: A
authors.lastname: Lavanya
authors.forename: A Raksha
authors.initials: AR
authors.lastname: Ganesh
authors.forename: Ravindra M
authors.initials: RM
authors.lastname: Kumbhare
authors.forename: Kaustav
authors.initials: K
authors.lastname: Bhadra
authors.forename: Utpal
authors.initials: U
authors.lastname: Bhadra
authors.forename: Manika
authors.initials: M
authors.lastname: Pal-Bhadra
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D052160:Benzothiazoles / Q000737:chemistry* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000235:genetics; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry / Q000494:pharmacology; D035683:MicroRNAs / Q000235:genetics*
chemical_list: D000970:Antineoplastic Agents; D052160:Benzothiazoles; D007093:Imidazoles; D035683:MicroRNAs; C471146:bantam microRNA, Drosophila