title: Alterations in the detergent-induced membrane permeability and solubilization of saturated phosphatidylcholine/cholesterol liposomes: effects of poly(ethylene glycol)-conjugated lipid.
abstract: We have investigated the effects of two bile salts, chenodeoxycholate (CDC) and ursodeoxycholate (UDC), and a widely used detergent, Triton X-100 (T(X-100)), on normal and poly(ethylene glycol)-modified liposomes (PEGylated liposomes). We tested various lipid compositions, including hydrogenated soybean phosphatidylcholine/cholesterol/PEG-conjugated lipid (HSPC/PEG-lipid). Alterations in permeability were determined by the rate of drug release from the liposomes and solubilization was assessed by measuring the particle size of liposomes. In addition, we attempted to observe interactions between the detergents and lipid bilayers by using surface plasmon resonance (SPR). CDC induced drug release from liposomes in a dose-dependent manner, and the PEGylated liposomes tended to be susceptible to CDC. While UDC did not strongly induce drug release from liposomes, UDC exhibited a similar tendency with CDC. In case of T(X-100), there were significant differences in the percentage of released drug between normal and PEGylated liposomes, and the percentage of T(X-100)-induced drug release further increased with an increased ratio of PEG-lipid. SPR analysis revealed that the lipid bilayer including PEG-lipid was selectively solubilized by T(X-100), correlating with the drug release data. These results suggest that the effect of detergents on the lipid bilayer of liposomes depends on both the kind of detergent and the lipid composition, including the presence or absence of PEG-lipid. Moreover, the effects of T(X-100) on the lipid bilayers of the PEGylated liposomes significantly differed from those on the lipid bilayers of the normal liposomes.
authors.affiliation: National Institute of Health Sciences, 1-18-1 Kamiyoga, Tokyo 158-8501, Japan. h-shibata@nihs.go.jp
authors.forename: Hiroko
authors.initials: H
authors.lastname: Shibata
authors.forename: Haruna
authors.initials: H
authors.lastname: Saito
authors.forename: Chikako
authors.initials: C
authors.lastname: Yomota
authors.forename: Toru
authors.initials: T
authors.lastname: Kawanishi
authors.forename: Haruhiro
authors.initials: H
authors.lastname: Okuda
mesh_terms: D001647:Bile Acids and Salts / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism; D003902:Detergents / Q000378:metabolism*; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D017830:Octoxynol / Q000378:metabolism*; D010539:Permeability; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001647:Bile Acids and Salts; D003902:Detergents; D008081:Liposomes; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols; D017830:Octoxynol; D002784:Cholesterol