title: Construction of a rhythm transfer system that mimics the cellular clock.
abstract: Creation of an artificial oscillating gene expression system is one of the most challenging issues in synthetic biology. Here, we constructed a simple system to manipulate gene expression patterns to be circadian, reflecting the intrinsic cellular clock, by fusing a core clock protein, BMAL1 or CLOCK, with a zinc finger-type DNA binding domain. Circadian rhythmic gene expression was induced only when the target gene contained zinc finger-binding sequences. To our knowledge, this simple approach is the first to manipulate gene expression patterns into circadian rhythms and would be applicable to various endogenous genes.
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. imiki@scl.kyoto-u.ac.jp
authors.forename: Miki
authors.initials: M
authors.lastname: Imanishi
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamada
authors.forename: Yuka
authors.initials: Y
authors.lastname: Hirose
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Okamura
authors.forename: Shiroh
authors.initials: S
authors.lastname: Futaki
mesh_terms: D056930:ARNTL Transcription Factors / Q000235:genetics*; D000818:Animals; D056926:CLOCK Proteins / Q000235:genetics*; D002940:Circadian Rhythm; D017930:Genes, Reporter; D051379:Mice; D041681:NIH 3T3 Cells; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D015533:Transcriptional Activation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics; D016335:Zinc Fingers
chemical_list: D056930:ARNTL Transcription Factors; C538961:Bmal1 protein, mouse; D011993:Recombinant Fusion Proteins; D042461:Vascular Endothelial Growth Factor A; D056926:CLOCK Proteins