title: Effect of autologous platelet-rich plasma in combination with a biphasic synthetic graft material on bone healing in critical-size cranial defects.
abstract: OBJECTIVE
  The aim of the study was to investigate the effect of autologous platelet-rich plasma (PRP) on the osteogenic potential of a biphasic synthetic graft material composed of hydroxyapatite and beta-tricalcium phosphate (HA/β-TCP) in critical-size cranial defects in rabbits.
  
  
  METHODS
  Three circular bicortical critical-size cranial defects were created in each of 18 rabbits. The first of the defects was grafted with autologous PRP and HA/β-TCP, the second was grafted with HA/β-TCP without PRP, and the third was left unfilled as a negative control. Animals were euthanized at 2, 4, and 6 weeks after surgery. Harvested tissue specimens were evaluated histologically and histomorphometrically. Several parameters associated with osteoclastic and osteoblastic activities were measured and calculated. The results were statistically analyzed using the 1-way analysis of variance statistical method.
  
  
  RESULTS
  Histologic analysis of the samples showed bone tissue formation at all experimental sites including untreated control defects. A statistically significant difference in new bone formation between the defects treated with HA/β-TCP + PRP and defects treated with HA/β-TCP alone was not observed. Control untreated defects showed the greatest bone regeneration.
  
  
  CONCLUSIONS
  In this animal model, autologous PRP had no effect on bone healing in addition to a biphasic HA/β-TCP synthetic graft material after 2, 4, and 6 weeks of implantation.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Athens, Greece.
authors.forename: Gregory
authors.initials: G
authors.lastname: Faratzis
authors.forename: Minas
authors.initials: M
authors.lastname: Leventis
authors.forename: Evanthia
authors.initials: E
authors.lastname: Chrysomali
authors.forename: Lubna
authors.initials: L
authors.lastname: Khaldi
authors.forename: Areti
authors.initials: A
authors.lastname: Eleftheriadis
authors.forename: Iro
authors.initials: I
authors.lastname: Eleftheriadis
authors.forename: Ismene
authors.initials: I
authors.lastname: Dontas
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials; D006882:Hydroxyapatites / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects*; D053657:Platelet-Rich Plasma; D011817:Rabbits; D012886:Skull / Q000601:surgery*; D014182:Transplantation, Autologous
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate