title: Making the most of pathological specimens: molecular diagnosis in formalin-fixed, paraffin embedded tissue.
abstract: The development of commercial reagents designed specifically for use with formalin-fixed paraffin-embedded (FFPE) tissue has unlocked the diagnostic potential of this prolific resource. The availability of archival FFPE tissue and tissue from current patients make it an ideal resource for molecular testing. Despite its stability and ability to preserve morphological information, FFPE provides a number of technical challenges to the study of biomolecules. In particular, the cross-linking and processing present problems in the extraction and isolation of DNA, RNA and protein and affect their use in downstream analysis. Here we will discuss some of the problems of FFPE tissue, how they can be overcome and how FFPE material can be used within clinical molecular diagnostics.
authors.affiliation: Department of Laboratory Medicine, Edinburgh Royal Infirmary, Edinburgh, UK.
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Fairley
authors.forename: Katelyn
authors.initials: K
authors.lastname: Gilmour
authors.forename: Kathy
authors.initials: K
authors.lastname: Walsh
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D005404:Fixatives; D005557:Formaldehyde; D005821:Genetic Techniques; D023281:Genomics; D006801:Humans; D058990:Molecular Targeted Therapy; D009369:Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D016612:Paraffin Embedding; D057089:Pathology, Molecular / Q000379:methods*; D057285:Precision Medicine; D011237:Predictive Value of Tests; D011379:Prognosis; D040901:Proteomics; D016707:Tissue Fixation
chemical_list: D014408:Biomarkers, Tumor; D005404:Fixatives; D005557:Formaldehyde