title: Gene expression and DNA-methylation of bovine pretransfer endometrium depending on its receptivity after in vitro-produced embryo transfer.
abstract: Embryonic implantation to establish a pregnancy is a complex process that requires appropriate communication between the embryo and the maternal endometrium. Inadequate uterine receptivity may contribute to the majority of implantation failures. To provide a comprehensive inventory of genes and functional networks that represent the maternal input of the embryo-maternal cross-talk, a longitudinal, holistic study of the endometrial transcriptome in relation to the days of estrous and to the receptivity of the endometrium was performed in bovine. At day 3 of estrous, genes related to cell communication and mitochondrial energy metabolism were differentially expressed among high- and low-receptive endometria (HR, LR); at day 7, transcripts functioning in immune and inflammatory pathways, oxidative stress, and angiogenesis had different abundances. Additionally, temporal transcriptional changes between days 3 and 7 differed considerably among HR and LR. Further, several transcription factors were predicted as relevant for receptivity because they were either differentially expressed among HR and LR animals or are known to be associated with genes we detected to have differential expression. Finally, global DNA methylation varied according to the interaction of receptivity group and day of estrous, and a divergent trend, which correlated with abundance of DNMT1 transcript, was observed in LR and HR along the estrous cycle days. The study revealed that, even in early estrous, transcripts related to cell communication and response to exogenous stimuli, vascularization, and energy supply show divergent expression and longitudinal temporal regulation in HR and LR. Key components of these molecular pathways are known to be dependent on ovarian hormones that promote uterine receptivity.
authors.affiliation: Research Group 'Functional Genome Analysis', Leibniz Institute for Farm Animal Biology, Dummerstorf, Germany.
authors.forename: Siriluck
authors.initials: S
authors.lastname: Ponsuksili
authors.forename: Eduard
authors.initials: E
authors.lastname: Murani
authors.forename: Manfred
authors.initials: M
authors.lastname: Schwerin
authors.forename: Karl
authors.initials: K
authors.lastname: Schellander
authors.forename: Dawit
authors.initials: D
authors.lastname: Tesfaye
authors.forename: Klaus
authors.initials: K
authors.lastname: Wimmers
mesh_terms: D000818:Animals; D001706:Biopsy / Q000379:methods; D002417:Cattle; D002450:Cell Communication; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D019175:DNA Methylation; D004624:Embryo Transfer; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D044127:Epigenesis, Genetic; D030762:Estrous Cycle; D005260:Female; D020869:Gene Expression Profiling; D006728:Hormones / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008137:Longitudinal Studies; D008928:Mitochondria / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010053:Ovary / Q000378:metabolism; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D006728:Hormones; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; C000619380:DNMT1 protein, human