title: Direct reprogramming of terminally differentiated B cells into erythroid lineage.
abstract: Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Among various factors, GATA-1, SCL together with CCAAT/enhancer binding protein (C/EBP) α turned out to be a minimal set of factors that efficiently reprogrammed terminally differentiated mature B cells into erythroid lineage, as evidenced by colony forming assays and erythroid-specific gene expressions. This study sets an avenue to generate autologous erythrocytes from peripheral B cells.
authors.affiliation: Division of Hematology, Department of Internal Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Sadahira
authors.forename: Yumi
authors.initials: Y
authors.lastname: Fukuchi
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Kunimono
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Sakurai
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Ikeda
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Okamoto
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakajima
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D004912:Erythrocytes / Q000166:cytology; D041905:Erythroid Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014157:Transcription Factors / Q000235:genetics
chemical_list: D014157:Transcription Factors