title: Antioxidant capacity and angiotensin I converting enzyme inhibitory activity of a melon concentrate rich in superoxide dismutase.
abstract: Antioxidant capacity and angiotensin 1-converting enzyme (ACE) inhibitory activity of a melon concentrate rich in superoxide dismutase (SOD-MC) were investigated in vitro. The total antioxidant capacity (TAC) was measured by the Trolox equivalent antioxidant capacity assay (TEAC), the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, and the ferric reducing antioxidant power assay (FRAP). The ability of the extract to scavenge three specific reactive oxygen species (superoxide radical anion (O(2)(-)), hydroxyl radical (HO()) and hydrogen peroxide (H(2)O(2))) was also investigated in order to better evaluate its antioxidant properties. Even if the measures of TAC were relatively low, results clearly established an antioxidant potential of SOD-MC that exhibited the highest radical-scavenging activity towards O(2)(-), with a IC(50) 12-fold lower than that of H(2)O(2) or HO(). This lets hypothesis that the antioxidant potential of SOD-MC could be mainly due to its high level of SOD. Moreover, for the first time, an ACE inhibitory activity of SOD-MC (IC(50)=2.4±0.1mg/mL) was demonstrated, showing that its use as a functional food ingredient with potential preventive benefits in the context of hypertension may have important public health implications and should be carefully considered.
authors.affiliation: Joint Research Unit 204 NUTRIPASS, Prevention of Malnutritions & Linked Pathologies, Nutrition & Metabolism Team, University Montpellier South of France, Montpellier, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Carillon
authors.forename: Daniele
authors.initials: D
authors.lastname: Del Rio
authors.forename: Pierre-Louis
authors.initials: PL
authors.lastname: Teissèdre
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Cristol
authors.forename: Dominique
authors.initials: D
authors.lastname: Lacan
authors.forename: Jean-Max
authors.initials: JM
authors.lastname: Rouanet
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D019662:Cucurbitaceae / Q000737:chemistry* / Q000201:enzymology; D007703:Peptidyl-Dipeptidase A / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000975:Antioxidants; D010936:Plant Extracts; D010940:Plant Proteins; D013482:Superoxide Dismutase; D007703:Peptidyl-Dipeptidase A