title: Bioethanol production: an integrated process of low substrate loading hydrolysis-high sugars liquid fermentation and solid state fermentation of enzymatic hydrolysis residue.
abstract: An integrated process of enzymatic hydrolysis and fermentation was investigated for high ethanol production. The combination of enzymatic hydrolysis at low substrate loading, liquid fermentation of high sugars concentration and solid state fermentation of enzymatic hydrolysis residue was beneficial for conversion of steam explosion pretreated corn stover to ethanol. The results suggested that low substrate loading hydrolysis caused a high enzymatic hydrolysis yield; the liquid fermentation of about 200g/L glucose by Saccharomyces cerevisiae provided a high ethanol concentration which could significantly decrease cost of the subsequent ethanol distillation. A solid state fermentation of enzymatic hydrolysis residue was combined, which was available to enhance ethanol production and cellulose-to-ethanol conversion. The results of solid state fermentation demonstrated that the solid state fermentation process accompanied by simultaneous saccharification and fermentation.
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Qiulu
authors.initials: Q
authors.lastname: Chu
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Ma
authors.forename: Yong
authors.initials: Y
authors.lastname: Xu
authors.forename: Jia
authors.initials: J
authors.lastname: Ouyang
authors.forename: Junjun
authors.initials: J
authors.lastname: Zhu
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Yu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yong
mesh_terms: D056804:Biofuels / Q000032:analysis*; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012996:Solutions; D013696:Temperature; D013997:Time Factors; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D012996:Solutions; D000431:Ethanol; D001617:beta-Glucosidase; D002480:Cellulase