title: Protamine and fertilin mRNA: potential biomarkers of assisted reproductive technology outcomes.
abstract: We studied the relationship between the levels of protamines 1 and 2 (PRM1 and PRM2) and fertilin-β (ADAM-2) mRNA expression and outcomes of infertility treatment using assisted reproductive technologies was studied. Analysis of the relationships between the outcomes of in vitro fertilization and embryo transfer and profiles of the expression of seminal genes PRM1, PRM2, ADAM-2 mRNA, evaluated by reverse transcription quantitative PCR was carried out in 79 couples. Significant differences in the expression of seminal PRM1, PRM2, ADAM-2 mRNA were detected in couples with different outcomes of in vitro fertilization and embryo transfer. The levels of seminal gene expression are potential predictors of the efficiency of in vitro fertilization and embryo transfer.
authors.affiliation: V. I. Kulakov Center of Obstetrics, Gynecology, and Perinatology, the Ministry of Health and Social Development of the Russian Federation, Moscow, Russia.
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
authors.forename: O V
authors.initials: OV
authors.lastname: Bourmenskaya
authors.forename: V Yu
authors.initials: VY
authors.lastname: Smolnikova
authors.forename: O E
authors.initials: OE
authors.lastname: Krasnoschoka
authors.forename: D Yu
authors.initials: DY
authors.lastname: Trofimov
authors.forename: A E
authors.initials: AE
authors.lastname: Donnikov
authors.forename: E A
authors.initials: EA
authors.lastname: Kalinina
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D004624:Embryo Transfer; D000072196:Fertilins; D005307:Fertilization in Vitro; D006801:Humans; D007246:Infertility / Q000628:therapy*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D011479:Protamines / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018709:Statistics, Nonparametric; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D008562:Membrane Glycoproteins; C082073:PRM1 protein, human; D011479:Protamines; D012333:RNA, Messenger; C068621:protamine 2; D051722:ADAM Proteins; C000606454:ADAM2 protein, human; D000072196:Fertilins