title: The relationship between lignin peroxidase and manganese peroxidase production capacities and cultivation periods of mushrooms.
abstract: Mushrooms are able to secrete lignin peroxidase (LiP) and manganese peroxidase (MnP), and able to use the cellulose as sources of carbon. This article focuses on the relation between peroxidase-secreting capacity and cultivation period of mushrooms with non-laccase activity. Methylene blue and methyl catechol qualitative assay and spectrophotometry quantitative assay show LiP secreting unvaryingly accompanies the MnP secreting in mushroom strains. The growth rates of hyphae are detected by detecting the dry hyphal mass. We link the peroxidase activities to growth rate of mushrooms and then probe into the relationship between them. The results show that there are close relationships between LiP- and/or MnP-secretory capacities and the cultivation periods of mushrooms. The strains with high LiP and MnP activities have short cultivation periods. However, those strains have long cultivation periods because of the low levels of secreted LiP and/or MnP, even no detectable LiP and/or MnP activity. This study provides the first evidence on the imitate relation between the level of secreted LiP and MnP activities and cultivation periods of mushrooms with non-laccase activity. Our study has significantly increased the understanding of the role of LiP and MnP in the growth and development of mushrooms with non-laccase activity.
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, FuZhou 350002, China.
authors.forename: Jian Z
authors.initials: JZ
authors.lastname: Xu
authors.forename: Jun L
authors.initials: JL
authors.lastname: Zhang
authors.forename: Kai H
authors.initials: KH
authors.lastname: Hu
authors.forename: Wei G
authors.initials: WG
authors.lastname: Zhang
mesh_terms: D000363:Agaricales / Q000201:enzymology / Q000254:growth & development*; D007218:Industrial Microbiology; D010544:Peroxidases / Q000096:biosynthesis*
chemical_list: D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase