title: Inactivation or clearance of Coxiella burnetii in rat serum samples to enable safe serological testing.
abstract: To allow processing of serum samples from animals experimentally infected with Coxiella burnetii outside the BSL-3 facility, an inactivation or clearance protocol that does not hamper serological testing may be required. The effects of filtration (0.1 µm pore size), heating at 56 °C for 30 min, addition of NaN3 (0.09% w/v), and combinations thereof on the presence of viable C. burnetii as well as OD-values in ELISA were tested. Only filtration was shown to effectively clear all culturable C. burnetii.
authors.affiliation: Laboratory for Zoonoses and Environmental Microbiology, Centre for Infectious Disease Control, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
authors.forename: Marieke
authors.initials: M
authors.lastname: Opsteegh
authors.forename: Lianne
authors.initials: L
authors.lastname: de Heer
authors.forename: Harold
authors.initials: H
authors.lastname: van den Berg
authors.forename: Joke
authors.initials: J
authors.lastname: van der Giessen
mesh_terms: D000818:Animals; D016997:Coxiella burnetii / Q000187:drug effects* / Q000302:isolation & purification*; D004203:Disinfection / Q000379:methods*; D005374:Filtration / Q000379:methods; D006358:Hot Temperature; D050296:Microbial Viability / Q000187:drug effects*; D051381:Rats; D044967:Serum / Q000382:microbiology*; D019810:Sodium Azide / Q000633:toxicity; D013048:Specimen Handling / Q000379:methods*
chemical_list: D019810:Sodium Azide