title: Colorimetric detection of single-nucleotide polymorphisms with a real-time PCR-like sensitivity.
abstract: Coupling gold nanoparticle-based ligation to a ligation chain reaction enables colorimetric detection of single-nucleotide polymorphisms with a real-time PCR-like sensitivity. One mutant in 2000 copies of a wild-type gene can be detected in 20-100 pg of PCR-amplified genomic DNA samples.
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.forename: Huimin
authors.initials: H
authors.lastname: Deng
authors.forename: Alan Kay Liang
authors.initials: AK
authors.lastname: Teo
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Gao
mesh_terms: D003124:Colorimetry / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D006046:Gold; D004247:DNA