title: Preparation of chitooligosaccharides from Clanis bilineata larvae skin and their antibacterial activity.
abstract: Clanis bilineata larvae are widely consumed in China. In this study, chitooligosaccharides were prepared from C. bilineata larvae skin by demineralisation, deproteination, washing, drying, deacetylation, hydrolysis using commercial α-amylase, filtration, setting the preparation at approximately 15% (w/v), precipitation with 6 volumes of ethanol, and drying at 60°C for 2 h. The optimal hydrolysis conditions were determined as follows: pH 5.5; temperature, 55°C; enzyme amount, 40 mg/(g chitosan); reaction time, 4 h. The Fourier transform infrared spectra revealed that chitooligosaccharides with a degree of polymerisation in the range of 2-8 were the main component of the resulting product, with the chitooligosaccharide content and yield being 95.8% and 96.2% (w/w), respectively. The resulting product showed high antibacterial activity compared with the original chitosan.
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China. wushengjun008@sina.com
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D005591:Chemical Fractionation / Q000379:methods*; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D007814:Larva / Q000737:chemistry; D007915:Lepidoptera / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012867:Skin / Q000737:chemistry; D013696:Temperature; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D048271:Chitosan; D000516:alpha-Amylases