title: A novel fluorescent biosensor for detection of target DNA fragment from the transgene cauliflower mosaic virus 35S promoter.
abstract: In this paper, we reported a convenient fluorescence method for the detection of genetically modified organisms (GMOs). As it is known that the cauliflower mosaic virus (CaMV) 35S promoter is widely used in most transgenic plants (Schnurr and Guerra, 2000), we thus design a simple method based on the detection of a section target DNA (DNA-T) from the transgene CaMV 35S promoter. In this method, the full-length guanine-rich single-strand sequences were split into fragments (Probe 1 and 2) and each part of the fragment possesses two GGG repeats. In the presence of K(+) ion and berberine, if a complementary target DNA of the CaMV 35S promoter was introduced to hybridize with Probe 1 and 2, a G-quadruplex-berberine complex was thus formed and generated a strong fluorescence signal. The generation of fluorescence signal indicates the presence of CaMV 35S promoter. This method is able to identify and quantify Genetically Modified Organisms (GMOs), and it shows wide linear ranges from 5.0×10(-9) to 9.0×10(-7) mol/L with a detection limit of 2.0×10(-9) mol/L.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety Fuzhou University, Department of Chemistry, Fuzhou University, Fuzhou 350002, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Ya-shan
authors.initials: YS
authors.lastname: Zhang
authors.forename: Yi-bing
authors.initials: YB
authors.lastname: Lin
authors.forename: Yu-Jing
authors.initials: YJ
authors.lastname: Lu
authors.forename: Zhen-yu
authors.initials: ZY
authors.lastname: Lin
authors.forename: Kwok-Yin
authors.initials: KY
authors.lastname: Wong
authors.forename: Guo-nan
authors.initials: GN
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D017796:Caulimovirus / Q000235:genetics*; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D030821:Plants, Genetically Modified / Q000145:classification* / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D019076:Transgenes / Q000235:genetics
chemical_list: D018744:DNA, Plant; D004279:DNA, Viral