title: Isolation of powerful antioxidants from the medicinal halophyte Limoniastrum guyonianum.
abstract: The antioxidant capacities of the crude extract, aqueous and ethyl acetate partitions of Limoniastrum guyonianum Boiss. (Zita) were investigated in this study. The ethyl acetate phase exhibited a significant antioxidant activity as judged by total antioxidant activity, DPPH test and reducing power. Fractionation of this extract by centrifugal partition chromatography (CPC) using quaternary Arizona solvent systems composed of n-heptane/ethyl acetate/methanol/water led to ten fractions. The antioxidant capacities of these fractions were assessed using the same previous tests. Fraction 8 showed the highest antioxidant capacity (1291.1mg GAE/g DR), the power ability to quench DPPH radical (IC(50)=2μg/ml) and to reduce Fe(3+) (EC(50)=65μg/ml). From this fraction, three powerful flavonoids were isolated (1-3): gallocatechin (1), epigallocatechin (2) and epigallocatechin-3-O-gallate (3). These findings suggest that the antioxidative property of L. guynianum is may be related to the presence of these flavonoids, which can be used in various industrial fields.
authors.affiliation: Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cédria, BP 901, 2050 Hammam-Lif, Tunisia. najlatrabe3351@gmail.com
authors.forename: Najla
authors.initials: N
authors.lastname: Trabelsi
authors.forename: Samia
authors.initials: S
authors.lastname: Oueslati
authors.forename: Hanen
authors.initials: H
authors.lastname: Falleh
authors.forename: Pierre
authors.initials: P
authors.lastname: Waffo-Téguo
authors.forename: Yorgos
authors.initials: Y
authors.lastname: Papastamoulis
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mérillon
authors.forename: Chedly
authors.initials: C
authors.lastname: Abdelly
authors.forename: Riadh
authors.initials: R
authors.lastname: Ksouri
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D001130:Arizona; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry* / Q000235:genetics; D029606:Plumbaginaceae / Q000737:chemistry* / Q000235:genetics; D055051:Salt-Tolerant Plants
chemical_list: D000975:Antioxidants; D010936:Plant Extracts