title: Moderate red wine and grape juice consumption modulates the hydrolysis of the adenine nucleotides and decreases platelet aggregation in streptozotocin-induced diabetic rats.
abstract: This study investigated the ex vivo effects of the moderate red wine (RW) and grape juice (GJ) consumption, and the in vitro effects of the resveratrol, caffeic acid, gallic acid, quercetin, and rutin on NTPDase (nucleoside triphosphate diphosphohydrolase), ecto-nucleotide pyrophosphatase/phosphodiesterase (E-NPP), 5'-nucleotidase, and adenosine deaminase (ADA) activities in platelets and platelet aggregation from streptozotocin-induced diabetic rats. The animals were divided into six groups (n = 10): control/saline, control/GJ, control/RW, diabetic/saline, diabetic/GJ, and diabetic/RW. RW and GJ were administered for 45 days; after this period, the blood was collected for experimental determinations. Results showed that NTPDase, E-NPP, 5'-nucleotidase, and ADA activities as well as platelet aggregation were increased in the diabetic/saline group compared to the control/saline group. Treatment with RW and GJ increased ectonucleotidases activities and prevented the increase in the ADA activity in the diabetic/GJ and diabetic/RW groups. Platelet aggregation was also decreased by the treatment with RW and GJ in the diabetic/GJ and diabetic/RW groups. In the in vitro tests, resveratrol, caffeic acid, and gallic acid increased ATP, ADP, and AMP hydrolysis, while quercetin and rutin decreased the hydrolysis of these nucleotides in platelets of diabetic rats. The ADA activity and platelet aggregation were reduced in platelets of diabetic rats in the presence of all polyphenols tested in vitro. These findings suggest that RW, GJ, and all polyphenols tested were able to modulate the ectoenzymes activities. Moreover, a decrease in the platelet aggregation was observed and it could contribute to the prevention of platelet abnormality, and consequently vascular complications in diabetic state.
authors.affiliation: Departamento de Química/Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, Santa Maria, RS, Brazil. betaschmatz@hotmail.com
authors.forename: Roberta
authors.initials: R
authors.lastname: Schmatz
authors.forename: Thaís R
authors.initials: TR
authors.lastname: Mann
authors.forename: Roselia
authors.initials: R
authors.lastname: Spanevello
authors.forename: Michel M
authors.initials: MM
authors.lastname: Machado
authors.forename: Daniela
authors.initials: D
authors.lastname: Zanini
authors.forename: Victor C
authors.initials: VC
authors.lastname: Pimentel
authors.forename: Naiara
authors.initials: N
authors.lastname: Stefanello
authors.forename: Caroline C
authors.initials: CC
authors.lastname: Martins
authors.forename: Andréia M
authors.initials: AM
authors.lastname: Cardoso
authors.forename: Margarete
authors.initials: M
authors.lastname: Bagatini
authors.forename: Jessié
authors.initials: J
authors.lastname: Gutierres
authors.forename: Claudio A M
authors.initials: CA
authors.lastname: Leal
authors.forename: Luciane B
authors.initials: LB
authors.lastname: Pereira
authors.forename: Cinthia
authors.initials: C
authors.lastname: Mazzanti
authors.forename: Maria R
authors.initials: MR
authors.lastname: Schetinger
authors.forename: Vera M
authors.initials: VM
authors.lastname: Morsch
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D001628:Beverages; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D002109:Caffeic Acids / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006868:Hydrolysis / Q000187:drug effects; D008297:Male; D028321:Plant Preparations / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D011755:Pyrophosphatases / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D012431:Rutin / Q000494:pharmacology; D013267:Stilbenes / Q000494:pharmacology; D027843:Vitis / Q000737:chemistry*; D014920:Wine
chemical_list: D000227:Adenine Nucleotides; D000975:Antioxidants; D002109:Caffeic Acids; D028321:Plant Preparations; D013267:Stilbenes; D012431:Rutin; D011794:Quercetin; D015720:5'-Nucleotidase; D000243:Adenosine Deaminase; D011755:Pyrophosphatases; C418082:nucleoside triphosphate diphosphohydrolase 6; C021895:nucleoside triphosphate pyrophosphatase; D000077185:Resveratrol; C040048:caffeic acid