title: The cell walls of syncytia formed by Heterodera schachtii in Arabidopsis thaliana are abundant in methyl-esterified pectin.
abstract: Plant-parasitic cyst nematodes form a specialized feeding site, termed a syncytium, in the roots of host plants. Monoclonal antibodies to defined glycans, in addition to a cellulose-binding module, were used to characterize the cell walls of a functioning syncytia in situ. Cell walls of syncytia were found to contain cellulose, xyloglucan and mannan. Analysis of the pectin network revealed syncytial cell walls are abundant in homogalacturonan, which was heavily methyl-esterified. Arabinan was also detected and the results suggest the cell walls of syncytia are highly flexible.
authors.affiliation: Centre for Plant Sciences, University of Leeds, Leeds, UK. bgy4ljd@leeds.ac.uk
authors.forename: Laura Jane
authors.initials: LJ
authors.lastname: Davies
authors.forename: Peter E
authors.initials: PE
authors.lastname: Urwin
mesh_terms: D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism* / Q000469:parasitology; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism* / Q000469:parasitology; D002482:Cellulose / Q000378:metabolism; D015726:Giant Cells / Q000737:chemistry / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism; D008351:Mannans / Q000378:metabolism; D010368:Pectins / Q000737:chemistry / Q000378:metabolism*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism* / Q000469:parasitology; D014990:Xylans / Q000378:metabolism
chemical_list: D005936:Glucans; D008351:Mannans; D014990:Xylans; C029353:xyloglucan; D010368:Pectins; D002482:Cellulose; C003181:polygalacturonic acid