title: Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3.
abstract: The aim of the present study was to investigate the anti-tumor effects of a culture filtrate of Paecilomyces farinosus J3. Various anti-tumor assays using B16 melanoma cells were carried out. Paecilomyces farinosus J3 significantly decreased the wound healing capability, invasiveness and angiogenic activity, which was confirmed by wound healing, human umbilical vein endothelial cell and invasion assays. Paecilomyces farinosus J3 strongly inhibited cell migration, tube formation and the angiogenic process in a concentration-dependent manner. Zymographic analysis also indicated a reduced expression of matrix metalloproteinase-9 (MMP-9), a 92-kDa gelatinase. Taken together, the results indicate that the anti-tumor activities of Paecilomyces farinosus J3 originate from the reduction of MMP-9 expression in B16F10 cells.
authors.affiliation: Department of Food Science and Biotechnology;
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Chul-Hong
authors.initials: CH
authors.lastname: Park
authors.forename: Hyung-U
authors.initials: HU
authors.lastname: Son
authors.forename: Jin-Chul
authors.initials: JC
authors.lastname: Heo
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Nam
authors.forename: Kwang-Gil
authors.initials: KG
authors.lastname: Lee
authors.forename: Joo-Hong
authors.initials: JH
authors.lastname: Yeo
authors.forename: Cheol-Sik
authors.initials: CS
authors.lastname: Yoon
authors.forename: Jong-Myeung
authors.initials: JM
authors.lastname: Kim
authors.forename: Yong-Kyu
authors.initials: YK
authors.lastname: Shin
authors.forename: Si-Oh
authors.initials: SO
authors.lastname: Kim
authors.forename: Sang-Han
authors.initials: SH
authors.lastname: Lee