title: Mannose-binding lectin gene polymorphisms are not associated with susceptibility to severe early childhood caries.
abstract: Our purpose was to investigate a possible relationship between severe early childhood caries (S-ECC) and polymorphism of the mannose binding lectin gene and investigate the role of allele variant as a possible factor in the susceptibility to S-ECC. Sixty-two Chinese children with S-ECC and 68 caries-free control children were included in this study. Genomic DNA was extracted from buccal epithelial cells of each individual. The identification of MBL B allele was performed by restriction fragment length polymorphism using Ban I restriction enzyme. The frequency of MBL mutant genotype (GGC/GAC and GAC/GAC) was more frequent among children with S-ECC compared with control groups, but did not significantly differ between two groups (x(2) = 2.82, p > 0.05). There was no significant difference in the allele frequency of codon54 wild type (allele A) between two groups (x(2) = 2.76, p > 0.05). The present study did not find evidence of MBL codon54 polymorphisms being associated with S-ECC in the population studied, but a larger sample size is necessary to confirm the present results.
authors.affiliation: Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China. rubyea@sina.com.cn
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wang
authors.forename: Man
authors.initials: M
authors.lastname: Qin
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003062:Codon; D003731:Dental Caries / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012720:Severity of Illness Index; D013997:Time Factors
chemical_list: D003062:Codon; D037601:Mannose-Binding Lectin