title: Micro-RNA expression in the urinary sediment of patients with chronic kidney diseases.
abstract: BACKGROUND
  Evidence indicates that microRNAs (miRNA) play a role in the pathogenesis of chronic kidney diseases (CKD). We explored the possibility of using urinary miRNA as non-invasive biomarkers for CKD.
  
  
  METHODS
  We quantified miRNA expression in urinary sediment of 56 CKD patients who underwent kidney biopsy. Patients were followed for 16.2 ± 15.5 months.
  
  
  RESULTS
  Patients with diabetic glomerulosclerosis had lower urinary miR-15 expression, while those with IgA nephropathy had higher urinary miR-17 expression, than other diagnosis groups. Baseline proteinuria had significant inverse correlation with urinary expression of miR-15, miR-192, and miR-216a; baseline renal function correlated with urinary expression of miR-15, miR-17, miR-192, and miR-217. The rate of renal function decline correlated with urinary expression of miR-21 (r=0.301, p=0.026) and miR-216a (r=0.515, p < 0.0001). Patients with a high urinary expression of miR-21 and miR-216a had better dialysis-free survival than those with low expression (log rank test, p=0.005 and p=0.003, respectively).
  
  
  CONCLUSIONS
  Urinary miR-21 and miR-216a expression correlated with the rate of renal function decline and risk of progression to dialysis-dependent renal failure. Our results suggest that urinary miRNA profiling has the potential of further development as biomarkers of CKD.
authors.affiliation: Department of Medicine & Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, China. ccszeto@cuhk.edu.hk
authors.forename: Cheuk-Chun
authors.initials: CC
authors.lastname: Szeto
authors.forename: Kwan Bonnie
authors.initials: KB
authors.lastname: Ching-Ha
authors.forename: Lai
authors.initials: L
authors.lastname: Ka-Bik
authors.forename: Lai Fernand
authors.initials: LF
authors.lastname: Mac-Moune
authors.forename: Choi Paul
authors.initials: CP
authors.lastname: Cheung-Lung
authors.forename: Wang
authors.initials: W
authors.lastname: Gang
authors.forename: Chow
authors.initials: C
authors.lastname: Kai-Ming
authors.forename: Li Philip
authors.initials: LP
authors.lastname: Kam-Tao
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine; D003928:Diabetic Nephropathies / Q000175:diagnosis / Q000235:genetics / Q000652:urine; D005260:Female; D015870:Gene Expression; D005922:Glomerulonephritis, IGA / Q000175:diagnosis / Q000235:genetics / Q000652:urine; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000652:urine*; D008875:Middle Aged; D011379:Prognosis; D011507:Proteinuria / Q000235:genetics / Q000652:urine; D051436:Renal Insufficiency, Chronic / Q000175:diagnosis / Q000235:genetics / Q000652:urine*
chemical_list: D015415:Biomarkers; C524940:MIRN21 microRNA, human; C575482:MIRN216 microRNA, human; D035683:MicroRNAs