title: Enhancement of acetic acid tolerance in Saccharomyces cerevisiae by overexpression of the HAA1 gene, encoding a transcriptional activator.
abstract: Haa1 is a transcriptional activator required for Saccharomyces cerevisiae adaptation to weak acids. Here we show that the constitutive HAA1-overexpressing strain acquired a higher level of acetic acid tolerance. Under conditions of acetic acid stress, the intracellular level of acetic acid was significantly lower in HAA1-overexpressing cells than in the wild-type cells.
authors.affiliation: Research Division of Microbial Sciences, Kyoto University, Sakyo-ku, Kyoto, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanaka
authors.forename: Yukari
authors.initials: Y
authors.lastname: Ishii
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
authors.forename: Jun
authors.initials: J
authors.lastname: Shima
mesh_terms: D019342:Acetic Acid / Q000633:toxicity*; D018628:Gene Dosage; D015870:Gene Expression; D060847:Metabolic Engineering; D050296:Microbial Viability / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: C439059:Haa1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D019342:Acetic Acid