title: Isolation of rat adrenocortical mitochondria.
abstract: This report describes a relatively simple and reliable method for isolating adrenocortical mitochondria from rats in good, reasonably pure yield. These organelles, which heretofore have been unobtainable in isolated form from small laboratory animals, are now readily accessible. A high degree of mitochondrial purity is shown by the electron micrographs, as well as the structural integrity of each mitochondrion. That these organelles have retained their functional integrity is shown by their high respiratory control ratios. In general, the biochemical performance of these adrenal cortical mitochondria closely mirrors that of typical hepatic or cardiac mitochondria.
authors.affiliation: Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.
authors.forename: Paola
authors.initials: P
authors.lastname: Solinas
authors.forename: Hisashi
authors.initials: H
authors.lastname: Fujioka
authors.forename: Bernard
authors.initials: B
authors.lastname: Tandler
authors.forename: Charles L
authors.initials: CL
authors.lastname: Hoppel
mesh_terms: D000302:Adrenal Cortex / Q000648:ultrastructure*; D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D008297:Male; D008928:Mitochondria / Q000737:chemistry*; D051381:Rats; D011916:Rats, Inbred F344