title: Antiadipogenic activity of isohamnetin 3-O-β-D-glucopyranoside from Salicornia herbacea.
abstract: In the present study, effect of flavonoid glucopyranoside, isorhamnetin 3-О-β-D-glucopyranoside, from Salicornia herbacea on adipogenic differentiation were evaluated in 3T3-L1 adipocytes. Confluent 3T3-L1 preadipocytes in medium (0.5 mM methylisobutylxanthine, 0.25 µM dexamethasone, 5 µg/mL insulin, and 10% fetal bovine serum [FBS]) were differentiated into adipocytes for 6 days with/without isorhamnetin 3-О-β-D-glucopyranoside. The presence of isorhamnetin 3-О-β-D-glucopyranoside effectively suppressed adipogenic differentiation by downregulation of peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding proteins (C/EBPα), sterol regulatory element-binding protein 1 (SREBP1), and the adipocyte-specific proteins. Moreover, the specific mechanism mediating the effects of isorhamnetin 3-О-β-D-glucopyranoside was confirmed by activation of AMP-activated protein kinase (AMPK). These findings suggest that isorhamnetin 3-О-β-D-glucopyranoside exerts antiadipogenic activity through AMPK activation. This study should find the nutraceutical value of S. herbacea-derived glucopyranoside, isorhamnetin 3-О-β-D-glucopyranoside, as potent candidate of antiobesity agent via alleviation of lipid accumulation.
authors.affiliation: Department of Food and Nutrition, College of Medical and Life Science, Silla University, Busan, Republic of Korea.
authors.forename: Chang-Suk
authors.initials: CS
authors.lastname: Kong
authors.forename: Youngwan
authors.initials: Y
authors.lastname: Seo
mesh_terms: D041721:3T3-L1 Cells; D000091162:AMP-Activated Protein Kinase Kinases; D017667:Adipocytes / Q000378:metabolism* / Q000473:pathology; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D019663:Chenopodiaceae / Q000737:chemistry*; D015536:Down-Regulation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D044948:Flavonols / Q000737:chemistry / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D011494:Protein Kinases / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis
chemical_list: D000943:Antigens, Differentiation; D044948:Flavonols; D006027:Glycosides; D014157:Transcription Factors; C432511:isorhamnetin 3-O-glucoside; D011494:Protein Kinases; D000091162:AMP-Activated Protein Kinase Kinases