title: Bovine milk fat globule membrane affects virulence expression in Escherichia coli O157:H7.
abstract: The aim of this study was to examine the effect of the bovine milk fat globule membrane (MFGM) on the virulence of Escherichia coli O157:H7. The MFGM was extracted from raw or heat-treated milk, resulting in 2 preparations differing in protein composition. Both heated and raw MFGM exerted an inhibitory effect on Shiga toxin gene expression by E. coli O157:H7 (ratios of -7.69 and -5.96, respectively). Interestingly, the effect was stronger with heated MFGM, with a larger decrease in expression of the virulence gene fliC (ratio of -9.43). The difference in effect observed between heated and raw MFGM could be explained by the difference in protein composition between the 2 preparations. These results show, for the first time, a specific effect of MFGM on expressionof Shiga toxin genes as well as genes involved in the motility of E. coli O157:H7. This may offer a new approach to mitigate the adverse health effects caused by E. coli O157:H7 infections.
authors.affiliation: Department of Food Science; Canadian Research Institute for Food Safety, University of Guelph, Guelph, ON, N1G 2W1, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Tellez
authors.forename: M
authors.initials: M
authors.lastname: Corredig
authors.forename: A
authors.initials: A
authors.lastname: Guri
authors.forename: R
authors.initials: R
authors.lastname: Zanabria
authors.forename: M W
authors.initials: MW
authors.lastname: Griffiths
authors.forename: V
authors.initials: V
authors.lastname: Delcenserie
mesh_terms: D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D004927:Escherichia coli Infections / Q000382:microbiology; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000472:pathogenicity; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005408:Flagellin; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D006017:Glycolipids / Q000032:analysis / Q000494:pharmacology*; D006023:Glycoproteins / Q000032:analysis / Q000494:pharmacology*; D006358:Hot Temperature; D066292:Lipid Droplets; D008566:Membranes / Q000378:metabolism; D008892:Milk / Q000382:microbiology; D008894:Milk Proteins / Q000032:analysis / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014774:Virulence / Q000187:drug effects
chemical_list: D029968:Escherichia coli Proteins; C516095:FliC protein, E coli; D006017:Glycolipids; D006023:Glycoproteins; D008894:Milk Proteins; C110367:milk fat globule; D005408:Flagellin