title: Increase of electronegative-LDL-fraction ratio and IDL-cholesterol in chronic kidney disease patients with hemodialysis treatment.
abstract: BACKGROUND
  It is known that the increased level of IDL and oxidized LDL are associated with risk of cardiovascular disease, and the lipoprotein abnormalities accelerate atherosclerosis. Cardiovascular disease is a major cause of mortality in chronic kidney disease patients with hemodialysis treatment (HD-Ps). Therefore, the estimation of lipoprotein profiles is important for prevention of cardiovascular disease in HD-Ps. We previously established an anion-exchange chromatographic method for measurement of cholesterol level in subclasses of HDL and LDL, IDL, VLDL, and chylomicron. An electronegative-LDL-fraction contained minimally oxidized-LDL. Lipoprotein profile can be accurately and conveniently determined by the new method.
  
  
  RESULTS
  In this study, lipoprotein profiles in HD-Ps and age-matched healthy subjects were estimated by using our established anion-exchange chromatographic method. The ratio of electronegative-LDL-cholesterol to total LDL-cholesterol and IDL-cholesterol in HD-Ps were significant higher than those in healthy subjects.
  
  
  CONCLUSIONS
  The results suggest that the ratio of electronegative-LDL-cholesterol to total LDL-cholesterol and IDL-cholesterol obtained by the new method may serve as useful markers for risk of cardiovascular disease in HD-Ps.
authors.affiliation: Bioscience Division, Tosoh Corporation, 2743-1 Hayakawa Ayase-shi, Kanagawa, Japan. yuuji-hirowatari-bx@tosoh.co.jp
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hirowatari
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Homma
authors.forename: Joe
authors.initials: J
authors.lastname: Yoshizawa
authors.forename: Koichiro
authors.initials: K
authors.lastname: Homma
mesh_terms: D000368:Aged; D053283:Apolipoprotein B-48 / Q000097:blood; D002318:Cardiovascular Diseases / Q000150:complications / Q000378:metabolism; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood; D015243:Cholesterol, VLDL / Q000097:blood; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D051436:Renal Insufficiency, Chronic / Q000150:complications / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
chemical_list: D053283:Apolipoprotein B-48; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D008075:Lipoproteins, HDL; D014280:Triglycerides