title: CE analysis of γ-globulin mobility and potential clinical utility.
abstract: Serum protein electrophoresis is widely used in clinical laboratories to measure the relative abundance of each obtained fraction. Moreover, we found that the migration time of the γ-globulin fraction can be reproducibly determined (CV = 1.1%). Immunoglobulins were purified from serum using protein L-agarose and their N-glycosylation was studied using CE on a DNA sequencer. Liver fibrosis patients showed a lower level of sialylation and this moderately correlates with the migration time of the γ-globulins (r = 0.2-0.4). This allowed us to differentiate healthy individuals from these patients with an acceptable diagnostic accuracy (area under the curve = 0.75). This glycomics approach could become a significant added value to a daily, routine clinical test.
authors.affiliation: Department for Molecular Biomedical Research, Unit for Molecular Glycobiology, VIB, Ghent, Belgium.
authors.forename: Dieter
authors.initials: D
authors.lastname: Vanderschaeghe
authors.forename: Evi
authors.initials: E
authors.lastname: Debruyne
authors.forename: Hans
authors.initials: H
authors.lastname: Van Vlierberghe
authors.forename: Nico
authors.initials: N
authors.lastname: Callewaert
authors.forename: Joris
authors.initials: J
authors.lastname: Delanghe
mesh_terms: D001798:Blood Proteins / Q000302:isolation & purification; D002624:Chemistry, Clinical / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006031:Glycosylation; D006801:Humans; D007136:Immunoglobulins / Q000302:isolation & purification; D011134:Polysaccharides / Q000378:metabolism; D005719:gamma-Globulins / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D007136:Immunoglobulins; D011134:Polysaccharides; D005719:gamma-Globulins