title: Implementation of a genetically tuned neural platform in optimizing fluorescence from receptor-ligand binding interactions on microchips.
abstract: This paper describes the use of a genetically tuned neural network platform to optimize the fluorescence realized upon binding 5-carboxyfluorescein-D-Ala-D-Ala-D-Ala (5-FAM-(D-Ala)(3) ) (1) to the antibiotic teicoplanin from Actinoplanes teichomyceticus electrostatically attached to a microfluidic channel originally modified with 3-aminopropyltriethoxysilane. Here, three parameters: (i) the length of time teicoplanin was in the microchannel; (ii) the length of time 1 was in the microchannel, thereby, in equilibrium with teicoplanin, and; (iii) the amount of time buffer was flushed through the microchannel to wash out any unbound 1 remaining in the channel, are examined at a constant concentration of 1, with neural network methodology applied to optimize fluorescence. Optimal neural structure provided a best fit model, both for the training set (r(2) = 0.985) and testing set (r(2) = 0.967) data. Simulated results were experimentally validated demonstrating efficiency of the neural network approach and proved superior to the use of multiple linear regression and neural networks using standard back propagation.
authors.affiliation: Department of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA.
authors.forename: Judith
authors.initials: J
authors.lastname: Alvarado
authors.forename: Grady
authors.initials: G
authors.lastname: Hanrahan
authors.forename: Huong T H
authors.initials: HT
authors.lastname: Nguyen
authors.forename: Frank A
authors.initials: FA
authors.lastname: Gomez
mesh_terms: D003198:Computer Simulation; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D016014:Linear Models; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D008957:Models, Genetic; D016571:Neural Networks, Computer; D009842:Oligopeptides / Q000737:chemistry; D011485:Protein Binding; D015203:Reproducibility of Results; D055672:Static Electricity; D017334:Teicoplanin / Q000737:chemistry / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D009842:Oligopeptides; C542686:carboxyfluorescein-D-Ala-D-Ala-D-Ala; D017334:Teicoplanin