title: Paraoxonase-1 gene Q192R polymorphism and reactive oxygen metabolites.
abstract: OBJECTIVE
  Paraoxonase-1 (PON1) is a high-density lipoprotein-associated antioxidant enzyme. The Q192R polymorphism of the PON1 gene can protect against oxidative conditions, but the relationship between Q192R polymorphism and oxidative stress-related markers remains controversial.  In this study, the diacron reactive oxygen metabolites (d-ROMs) test was used to investigate the relationship between Q192R polymorphism and oxidative stress-related markers in Japanese subjects.
  
  
  METHODS
  Patients without a history of overt cardiovascular disease who were not receiving antioxidant  medication were enrolled in a cross-sectional clinic-based study. An allele-specific polymerase chain reaction method was used to assess the PON1 Q192R polymorphism and compare the level of d-ROMs between genotypes.
  
  
  RESULTS
  A total of 103 subjects were analysed. The RR genotype was  associated with a significantly lower level of d-ROMs than the RQ and QQ genotypes. After multivariate analysis the relationship between the genotypes and level of d-ROMs remained independently significant.
  
  
  CONCLUSIONS
  The RR genotype may be protective against oxidative stress in cardiovascular  diseasefree Japanese subjects. In addition, the d-ROMs test can be useful for examining the role of the PON1 Q192R polymorphism under oxidative conditions.
authors.affiliation: Division of Preventive Medicine, Clinical Research Institute, National Hospital Organization Kyoto Medical Centre, 1-1 Fukakusa mukaihata, Fushimi-ku, Kyoto 612-8555, Japan. kazukotani@jichi.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Kotani
authors.forename: K
authors.initials: K
authors.lastname: Tsuzaki
authors.forename: N
authors.initials: N
authors.lastname: Sakane
mesh_terms: D019943:Amino Acid Substitution; D043303:Aryldialkylphosphatase / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D018384:Oxidative Stress; D011110:Polymorphism, Genetic; D017382:Reactive Oxygen Species / Q000097:blood*
chemical_list: D017382:Reactive Oxygen Species; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human