title: Ring test evaluation of the repeatability and reproducibility of a Porcine reproductive and respiratory syndrome virus oral fluid antibody enzyme-linked immunosorbent assay.
abstract: The precision of a Porcine reproductive and respiratory syndrome virus (PRRSV) oral fluid antibody enzyme-linked immunosorbent assay (ELISA) was evaluated by calculating reliability coefficients for assay repeatability (within laboratory) and assay reproducibility (between laboratories). Randomly ordered oral fluid samples of known (n = 39) and unknown (n = 224) PRRSV antibody status were tested in 12 diagnostic laboratories. Each laboratory tested the samples twice, first using an antibody ELISA kit and reagents provided to them (phase 1) and then using an ELISA kit and reagents configured in their respective laboratory (phase 2). Repeatability (within laboratory) reliability coefficients calculated using results from samples of known PRRSV antibody status ranged from 0.724 to 0.997 in phase 1 and from 0.953 to 0.998 in phase 2. Reproducibility (between laboratories) reliability coefficients were calculated for 3 conditions: case 1--samples of unknown status (n = 224); case 2--samples of known status (n = 39), and case 3--all samples (n = 263). Among the 3 cases, reliability coefficients ranged from 0.937 to 0.964 in phase 1 and from 0.922 to 0.935 in phase 2. For case 3, it was estimated that 96.67% of the total variation in phase 1 and 93.21% in phase 2 could be attributed to the oral fluid samples themselves. Overall, the PRRSV oral fluid antibody ELISA was highly repeatable and reproducible. The current study supports the routine use of this test in laboratories providing diagnostic service to pig producers.
authors.affiliation: Veterinary Diagnostic Laboratory, Iowa State University, Ames, IA, USA. jjzimm@iastate.edu
authors.forename: Apisit
authors.initials: A
authors.lastname: Kittawornrat
authors.forename: Chong
authors.initials: C
authors.lastname: Wang
authors.forename: Gary
authors.initials: G
authors.lastname: Anderson
authors.forename: Andrea
authors.initials: A
authors.lastname: Ballagi
authors.forename: André
authors.initials: A
authors.lastname: Broes
authors.forename: Suzanne
authors.initials: S
authors.lastname: Carman
authors.forename: Kent
authors.initials: K
authors.lastname: Doolittle
authors.forename: Judith
authors.initials: J
authors.lastname: Galeota
authors.forename: John
authors.initials: J
authors.lastname: Johnson
authors.forename: Sergio
authors.initials: S
authors.lastname: Lizano
authors.forename: Eric
authors.initials: E
authors.lastname: Nelson
authors.forename: Devi
authors.initials: D
authors.lastname: Patnayak
authors.forename: Roman
authors.initials: R
authors.lastname: Pogranichniy
authors.forename: Anna
authors.initials: A
authors.lastname: Rice
authors.forename: Gail
authors.initials: G
authors.lastname: Scherba
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Zimmerman
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D015203:Reproducibility of Results; D012463:Saliva / Q000276:immunology*; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D000914:Antibodies, Viral