title: Surface roughness and charge influence the uptake of nanoparticles: fluorescently labeled pickering-type versus surfactant-stabilized nanoparticles.
abstract: The influence of surface roughness and charge on the cellular uptake of nanoparticles in HeLa cells is investigated with fluorescent, oppositely charged, rough, and smooth nanoparticles. Flow cytometry, cLSM, and TEM reveal that rough nanoparticles are internalized by the cells more slowly and by an unidentified uptake route as no predominant endocytosis route is blocked by a variety of inhibitory drugs, while the uptake of smooth nanoparticles is strongly dependent on dynamin, F-actin, and lipid-raft. Negatively charged nanoparticles are taken up to a higher extent than positively charged ones, independent of the surface roughness.
authors.affiliation: Institute of Organic Chemistry III - Macromolecular Chemistry and Organic Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
authors.forename: Anika
authors.initials: A
authors.lastname: Schrade
authors.forename: Volker
authors.initials: V
authors.lastname: Mailänder
authors.forename: Sandra
authors.initials: S
authors.lastname: Ritz
authors.forename: Katharina
authors.initials: K
authors.lastname: Landfester
authors.forename: Ulrich
authors.initials: U
authors.lastname: Ziener
mesh_terms: D000199:Actins / Q000378:metabolism; D001692:Biological Transport; D034281:Dynamins / Q000378:metabolism; D004655:Emulsions; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006367:HeLa Cells; D006801:Humans; D021962:Membrane Microdomains / Q000737:chemistry / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D058105:Polymerization; D012822:Silicon Dioxide / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D000199:Actins; D004655:Emulsions; D005456:Fluorescent Dyes; D013501:Surface-Active Agents; D012822:Silicon Dioxide; D034281:Dynamins