title: Phenotypic and molecular characteristics of plaque-purified MX10 virus, an Oriental-Australian genotype of Sindbis virus from Yunnan, China.
abstract: A previous investigation showed that MX10 virus, recently isolated in China, belongs to the Oriental-Australian (O/A) genotype of Sindbis virus (SINV) (Wang Jinglin, 2011, ATMH). Similar to the MRE16 isolate, the prototype O/A genotype of SINV, two derivate viruses with obviously different plaque morphologies were derived from MX10 virus, which were accordingly denoted as MX10-LP and MX10-SP. MX10-LP virus exhibited higher neurovirulence in neonatal mice than MX10-SP virus. Analysis of the complete genome revealed seven nucleotide differences between MX10-LP and MX10-SP. Compared with MRE16 virus, MRE16SP virus has a deletion of 30 aa in the E2 gene (200-229), which has been shown to be the molecular basis for the different plaque morphology. However, the MX10-SP virus did not have the 30-amino-acid deletion in the E2 gene. These results demonstrate that the molecular basis for the different plaque morphology of MX10 virus, the first strain of the O/A genotype of SINV isolated from China, is different from that of the prototype MRE16 virus.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Viral Disease Control and Prevention, Beijing 100052, People's Republic of China.
authors.forename: Wang
authors.initials: W
authors.lastname: Jinglin
authors.forename: Zhu
authors.initials: Z
authors.lastname: Wuyang
authors.forename: Shihong
authors.initials: S
authors.lastname: Fu
authors.forename: Li
authors.initials: L
authors.lastname: Wenjuan
authors.forename: Zhang
authors.initials: Z
authors.lastname: Hailin
authors.forename: Guodong
authors.initials: G
authors.lastname: Liang
mesh_terms: D000818:Animals; D001315:Australia; D002460:Cell Line; D002681:China; D003465:Culex / Q000821:virology*; D005838:Genotype; D051379:Mice; D010641:Phenotype; D012845:Sindbis Virus / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology; D010948:Viral Plaque Assay