title: Neuropathic pain and reactive gliosis are reversed by dialdehydic compound in neuropathic pain rat models.
abstract: The role of the purinergic system in the modulation of pain mechanisms suggests that it might be promising target for treating neuropathic pain. In this study we evaluated the effects of two different dialdehydic compounds: a modified stable adenosine (2-[1-(6-amminopurin-9-il)-2-osso-etossi]prop-2-enale, named MED1101), and oxidized ATP (Ox-ATP), in two different neuropathic pain rat models: the sciatic spared nerve injury (SNI) and paclitaxel evoked painful peripheral neuropathy (pPPN). Neuropathic animals were divided in groups as follows: (a) treated with intraperitoneal (i.p.) MED1101 or Ox-ATP for 21 days; (b) receiving vehicle (VEH) and (c) control (CTR) rats. The allodynic and hyperalgesic behavior was investigated by Von Frey filament test and thermal Plantar test, respectively. We evaluated by immunocytochemistry the astrocytic (GFAP) and microglial (Iba1) response on lumbar spinal cord sections. In either experimental models and using either substances, treated animals showed reduced allodynia and thermal hyperalgesia paralleled by a significant reduction of glial reaction in the spinal cord. These data prompt to hypothesize a potential role of dialdehydes as analgesic agent in chronic neuropathic pain and a possible role as anti-gliotic molecules.
authors.affiliation: Dipartimento di Medicina Pubblica Clinica e Preventiva, Seconda Università di Napoli, 80138 Naples, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Bianco
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cirillo
authors.forename: Valentina
authors.initials: V
authors.lastname: Petrosino
authors.forename: Lorenza
authors.initials: L
authors.lastname: Marcello
authors.forename: Antonio
authors.initials: A
authors.lastname: Soleti
authors.forename: Giulia
authors.initials: G
authors.lastname: Merizzi
authors.forename: Carlo
authors.initials: C
authors.lastname: Cavaliere
authors.forename: Michele
authors.initials: M
authors.lastname: Papa
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000345:Affinity Labels / Q000494:pharmacology; D000447:Aldehydes / Q000494:pharmacology*; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000473:pathology; D002908:Chronic Disease; D004195:Disease Models, Animal; D055808:Drug Discovery / Q000379:methods; D005911:Gliosis / Q000188:drug therapy* / Q000473:pathology; D006930:Hyperalgesia / Q000188:drug therapy / Q000473:pathology; D008159:Lumbar Vertebrae; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000473:pathology; D009437:Neuralgia / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology
chemical_list: C580749:2-(1-(6-aminopurin-9-yl)-2-oxoethoxy)prop-2-enal; D000345:Affinity Labels; D000447:Aldehydes; D000700:Analgesics; C017199:2',3'-dialdehyde ATP; D000255:Adenosine Triphosphate; D000241:Adenosine