title: Screening of probiotic lactobacilli for inhibition of Shigella sonnei and the macromolecules involved in inhibition.
abstract: A total of 91 lactobacilli were screened for antimicrobial activity against Shigella sonnei. Agar-well assay showed that 16 lactobacilli displayed strong antibacterial activity against S. sonnei. The nature of these antimicrobial agents were investigated and shown to be dependent on their production of organic acids. Adhesion tests showed that 6 lactobacilli demonstrated good adherence to HT-29 cells, of these Lactobacillus johnsonii F0421 were selected for acid and bile salt tolerance properties. We further research on L. johnsonii F0421 inhibition of S. sonnei adhesion to HT-29 cells. The result showed that L. johnsonii F0421 exhibited significant inhibitory activity and excluded, competed and displaced adhered S. sonnei by 48%, 38% and 33%, respectively. In order to elucidate the inhibitory functions of macromolecules involved in L. johnsonii F0421, the cells were treated with 5 M LiCl, 0.05 M sodium metaperiodate and heating and assayed for inhibition activity. The results suggested a role of S-layer proteins on L. johnsonii F0421 cells in inhibition of the adhesion process, but carbohydrates do not seem to be involved. SDS-PAGE analysis confirmed the presence of S-layer proteins with dominant bands of approximately 40 kDa. In addition, 100 μg/well of S-layer proteins from L. johnsonii F0421 cells were effective in inhibiting adhesion of S. sonnei to HT-29 cells. These findings suggest that L. johnsonii F0421 possesses the capacity for inhibition of S. sonnei activity as well as probiotic properties, which could serve as a potential novel and effective probiotic strain for use in the food industry.
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Zhang
authors.forename: Lan-Wei
authors.initials: LW
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Hua-Xi
authors.initials: HX
authors.lastname: Yi
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Ming
authors.initials: M
authors.lastname: Du
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Shan
authors.forename: Xue
authors.initials: X
authors.lastname: Han
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism*; D000898:Antibiosis; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells / Q000382:microbiology; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D046911:Macromolecular Substances / Q000302:isolation & purification / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D019936:Probiotics; D012764:Shigella sonnei / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D046911:Macromolecular Substances