title: Accumulation of ACE inhibitory tripeptides, Val-Pro-Pro and Ile-Pro-Pro, in vascular endothelial cells.
abstract: The antihypertensive peptides, Val-Pro-Pro and Ile-Pro-Pro, were successfully detected in the aorta of spontaneously hypertensive rats after orally administering these peptides by a guanidine-thiocyanate treatment to prevent proteolysis. Cy3-labeled versions of both peptides were localized in the endothelial cells of arterial vessels in the rats. The accumulation of both peptides in the endothelial cells suggested in vivo inhibitory activity of the angiotensin I-converting enzyme.
authors.affiliation: Microbiology and Fermentation Laboratory, Calpis Co., Ltd., 5-11-10 Fuchinobe, Sagamihara, Kanagawa 252-0206, Japan.
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Kawaguchi
authors.forename: Teppei
authors.initials: T
authors.lastname: Nakamura
authors.forename: Junichi
authors.initials: J
authors.lastname: Kamiie
authors.forename: Toyomi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D001158:Arteries / Q000187:drug effects* / Q000201:enzymology; D001794:Blood Pressure / Q000187:drug effects; D002232:Carbocyanines; D042783:Endothelial Cells / Q000187:drug effects* / Q000201:enzymology; D006146:Guanidines / Q000737:chemistry; D006973:Hypertension / Q000188:drug therapy* / Q000201:enzymology; D007553:Isotope Labeling; D008297:Male; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D013861:Thiocyanates / Q000737:chemistry
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D002232:Carbocyanines; D006146:Guanidines; D009842:Oligopeptides; D013861:Thiocyanates; C089187:cyanine dye 3; C489032:isoleucyl-prolyl-proline; C489031:valyl-prolyl-proline; C054436:guanidine thiocyanate; D007703:Peptidyl-Dipeptidase A