title: Inhibition of mouse urinary bladder carcinogenesis by açai fruit (Euterpe oleraceae Martius) intake.
abstract: Açai, fruit from Euterpe oleraceae Martius, is consumed in natura and in a variety of beverages and food preparations and possesses several potential antioxidant compounds. In a first study for anticarcinogenicity screening, male Swiss mice (n = 20/per group) were chemically-induced to urothelial bladder carcinogenesis for 10 weeks and received a standard diet or a standard diet containing 2.5 and 5 % spray-dried açai pulp (AP) for 10 weeks. At week 20, the incidence of simple and nodular hyperplasia and the incidence and multiplicity of transitional cell carcinoma (TCC) were evaluated. In a second study for antigenotoxicity screening, male Swiss mice (n = 6/per group) were fed standard diet or standard diet containing 5 % AP for three weeks. Urothelial cell suspensions were obtained and challenged with H(2)O(2) for induction of DNA damage and analyzed by comet assay. Overall, dietary 5 % AP reduced TCC incidence and multiplicity (p = 0.019 and p = 0.015, respectively) and tumor cell proliferation and p63 expression (p = 0.02 and p = 0.007, respectively), Furthermore, the group fed the 5 % AP presented a significant reduction (p < 0.01) in DNA damage induced by H(2)O(2), a notable oxidant agent. The results suggest that the spray-dried açai pulp used here inhibits the TCC development in male Swiss mice, probably due to its potential antioxidant action.
authors.affiliation: Post-Graduation Program in Pathology, Faculty of Medicine, UNESP Sao Paulo State University, Botucatu, SP, Brazil.
authors.forename: Mariana F
authors.initials: MF
authors.lastname: Fragoso
authors.forename: Monize G
authors.initials: MG
authors.lastname: Prado
authors.forename: Luciano
authors.initials: L
authors.lastname: Barbosa
authors.forename: Noeme S
authors.initials: NS
authors.lastname: Rocha
authors.forename: Luis F
authors.initials: LF
authors.lastname: Barbisan
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology / Q000627:therapeutic use; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D028023:Arecaceae; D002295:Carcinoma, Transitional Cell / Q000378:metabolism / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004032:Diet; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D005638:Fruit; D006861:Hydrogen Peroxide; D006965:Hyperplasia; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010750:Phosphoproteins / Q000378:metabolism; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology / Q000627:therapeutic use; D015534:Trans-Activators / Q000378:metabolism; D001743:Urinary Bladder / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*
chemical_list: D016588:Anticarcinogenic Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D010750:Phosphoproteins; D028321:Plant Preparations; D015534:Trans-Activators; C486419:Trp63 protein, mouse; D006861:Hydrogen Peroxide