title: Immunomodulatory and antitumour effects of abnormal Savda Munziq on S180 tumour-bearing mice.
abstract: BACKGROUND
  Abnormal Savda Munziq (ASMq), a traditional uyghur medicine, has shown anti-tumour properties in vitro. This study attempts to confirm these effects in vivo and measure effects on the immune system.
  
  
  METHODS
  Kunming mice transplanted with Sarcoma 180 cells were treated with ASMq (2-8 g/kg/day) by intra-gastric administration compared to model and cyclophosphamide (20 mg/kg/day). After the 14th day post tumour implant, thymus, liver, spleen and tumours were removed, weighed, and processed for histopathological analysis. Blood samples were also taken for haematological and biochemical analyses including TNF-α , IL-1 β and IL-2. Splenic lymphocyte function was measured with MTT; lymphocyte subpopulations were measured by flow cytometry.
  
  
  RESULTS
  ASMq treated animals had reduced tumour volume compared to model and increased concentrations of TNF-α, IL-1β and IL-2 compared to untreated and to cyclophosphamide-treated animals. No histopathological alterations were observed. The absence of viable S180 cells and the presence of necrotic cells and granulation tissue were observed in tumour tissue of treated animals. The effect on T lymphocytes was unclear.
  
  
  CONCLUSIONS
  ASMq confirmed in vivo anti-tumour effects observed in vitro, which may be at least in part mediated by increased immune activity.
authors.affiliation: Department of Drug Analysis, Xinjiang Medical University, 830011 Urumqi, Xinjiang, People’s Republic of China.
authors.forename: Ainiwaer
authors.initials: A
authors.lastname: Aikemu
authors.forename: Anwar
authors.initials: A
authors.lastname: Umar
authors.forename: Abdiryim
authors.initials: A
authors.lastname: Yusup
authors.forename: Halmurat
authors.initials: H
authors.lastname: Upur
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Berké
authors.forename: Bernard
authors.initials: B
authors.lastname: Bégaud
authors.forename: Nicholas
authors.initials: N
authors.lastname: Moore
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D016207:Cytokines / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D007107:Immune System / Q000187:drug effects; D007155:Immunologic Factors / Q000008:administration & dosage*; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000276:immunology*
chemical_list: D000970:Antineoplastic Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors