title: Mapping the mechanism of the resorcinol ring formation catalyzed by ArsB, a type III polyketide synthase from Azotobacter vinelandii.
abstract: Who's first? Aldol cyclization occurs before hydrolysis in the resorcinol ring formation catalyzed by the type III polyketide synthase, ArsB. Synthetic C(20)-TKA was not converted to alkylresorcinol by ArsB, but rather inhibited the enzyme activity, thus indicating that C(20)-TKA is not an intermediate in ArsB-catalyzed alkylresorcinol formation.
authors.affiliation: Department of Chemistry and Biochemistry, University of Regina, 3737 Wascana Parkway, Regina, SK S4S 0A2, Canada.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Posehn
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Andrew G H
authors.initials: AG
authors.lastname: Wee
authors.forename: Dae-Yeon
authors.initials: DY
authors.lastname: Suh
mesh_terms: D016948:Azotobacter vinelandii / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D003500:Cyclization; D006868:Hydrolysis; D048630:Polyketide Synthases / Q000378:metabolism*; D012118:Resorcinols / Q000737:chemistry / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D012118:Resorcinols; D048630:Polyketide Synthases; C031389:resorcinol