title: The attenuation of cockroach allergy by DNA vaccine encoding cockroach allergen Bla g 2.
abstract: Bla g 2 is one of the most potent cockroach allergens. No effective treatment or vaccination strategies are yet available. We evaluated the prophylactic efficacy of Bla g 2 DNA vaccination in a mouse model of allergic airway inflammation. C57/BL6 mice were given Bla g 2 DNA vaccine prior to sensitization with recombinant Bla g 2 (rBla g 2) antigens, followed by nebulized rBla g 2 challenge. Bla g 2 vaccine could express at both transcriptional and translational levels in mammalian cells. Moreover, Bla g 2 vaccine significantly reduced the total inflammatory cell infiltrate and eosinophilia in bronchoalveolar lavage fluid, and markedly decreased allergen-induced inflammatory infiltrates in the lungs and Bla g 2-specific IgE in serum upon challenge with rBla g 2. Importantly, Bla g 2 vaccine could induce the production of antigen-specific IFN-γ and downregulated Th2 pro-inflammatory cytokines IL-4, IL-5, and IL-13. Thus, DNA vaccination showed protective efficacy against a clinically relevant allergen, Bla g 2.
authors.affiliation: Department of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Jingdong
authors.initials: J
authors.lastname: Yuan
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Alan W
authors.initials: AW
authors.lastname: James
authors.forename: Usha
authors.initials: U
authors.lastname: Nair
authors.forename: Xiji
authors.initials: X
authors.lastname: Shu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Siva
authors.initials: S
authors.lastname: Kanangat
authors.forename: Tai June
authors.initials: TJ
authors.lastname: Yoo
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000008:administration & dosage / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D045744:Cell Line, Tumor; D003058:Cockroaches / Q000276:immunology*; D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D005260:Female; D006967:Hypersensitivity / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D018793:Interleukin-13 / Q000097:blood / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000276:immunology; D015848:Interleukin-5 / Q000097:blood / Q000276:immunology; D007958:Leukocyte Count; D051379:Mice; D014162:Transfection; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D018793:Interleukin-13; D015848:Interleukin-5; D019444:Vaccines, DNA; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma; D016282:Aspartic Acid Endopeptidases; C094699:allergen Bla g 2