title: Encapsulation of photosensitizer into multilayer microcapsules by combination of spontaneous deposition and heat-induced shrinkage for photodynamic therapy.
abstract: Annealing of PDADMAC/PSS multilayer microcapsules assembled on PSS-doped CaCO(3) particles at 80 °C for 30 min reduces their size dramatically from 6.9 ± 0.3 to 3.1 ± 0.5 µm. Methylene blue molecules are encapsulated by spontaneous deposition and post-annealing with a concentration of 22 mg · mL(-1), which is 1000 times higher than the feeding value. The unreleased MB molecules are retained stably for a long time, which are then protected by the capsules against reductive enzymes and keep their photodynamic activity. The viability of HeLa cells incubated with the MB-loaded capsules decreases sharply from ≈ 75 (dark cytotoxicity) to ≈ 20% after irradiation with a laser at 671 nm and 60 J · cm(-2) for 75 s.
authors.affiliation: MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Han
authors.forename: Jing
authors.initials: J
authors.lastname: Bu
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Weijun
authors.initials: W
authors.lastname: Tong
authors.forename: Changyou
authors.initials: C
authors.lastname: Gao
mesh_terms: D002119:Calcium Carbonate / Q000737:chemistry; D002214:Capsules; D002470:Cell Survival / Q000187:drug effects; D004339:Drug Compounding; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D007834:Lasers; D008027:Light; D008751:Methylene Blue / Q000737:chemistry* / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry* / Q000494:pharmacology; D011095:Polyethylenes / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D002214:Capsules; D017319:Photosensitizing Agents; D011095:Polyethylenes; D011137:Polystyrenes; D000644:Quaternary Ammonium Compounds; D017382:Reactive Oxygen Species; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; C003321:polystyrene sulfonic acid; D002119:Calcium Carbonate; D008751:Methylene Blue