title: Application of whey protein micro-bead coatings for enhanced strength and probiotic protection during fruit juice storage and gastric incubation.
abstract: BACKGROUND
  Coated whey protein micro-beads may improve probiotic protection and provide delayed cell-release mechanisms.
  
  
  OBJECTIVE
  Lactobacillus rhamnosus GG was encapsulated in whey protein micro-beads by droplet extrusion with coating via electrostatic deposition: primary-polysaccharide and secondary-whey protein.
  
  
  METHODS
  Storage studies were performed in cranberry and pomegranate juice (pH 2.4; 28 days; 4 and 25°C) followed by simulated ex vivo porcine gastric (pH 1.6) and intestinal (pH 6.6) digestion.
  
  
  CONCLUSIONS
  After storage and simulated gastro-intestinal digestion, free cells, cells suspended in protein and cells encapsulated in alginate micro-beads, illustrated complete probiotic mortality, while coated micro-beads enhanced probiotic viability after juice storage (8.6 ± 0.1 log(10)CFUmL(-1)). Beads also showed significant binding of hydrophobic molecules. Coated micro-beads illustrated high gastric survival (9.5 ± 0.1 log(10)CFUmL(-1)) with 30 min delayed intestinal release relative to non-coated micro-beads.
  
  
  CONCLUSIONS
  Micro-bead coatings could be applied in delayed cell-release for targeted intestinal probiotic delivery.
authors.affiliation: Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
authors.forename: S B
authors.initials: SB
authors.lastname: Doherty
authors.forename: M A
authors.initials: MA
authors.lastname: Auty
authors.forename: C
authors.initials: C
authors.lastname: Stanton
authors.forename: R P
authors.initials: RP
authors.lastname: Ross
authors.forename: G F
authors.initials: GF
authors.lastname: Fitzgerald
authors.forename: A
authors.initials: A
authors.lastname: Brodkorb
mesh_terms: D000818:Animals; D001628:Beverages; D004063:Digestion; D004339:Drug Compounding; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D005519:Food Preservation; D005638:Fruit; D005753:Gastric Mucosa / Q000378:metabolism*; D008894:Milk Proteins; D019936:Probiotics; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D000067816:Whey Proteins