title: The rhizomes of Alisma orientale and alisol derivatives inhibit allergic response and experimental atopic dermatitis.
abstract: The 70% ethanol extract of the rhizome of Alisma orientale (Alismatis rhizome) (AOE) was prepared and found to significantly inhibit 5-lipoxygenase (5-LOX)-catalyzed leukotriene (LT) production from rat basophilic leukemia (RBL)-1 cells and β-hexosaminidase release by antigen-stimulated RBL-2H3 cells. It also attenuated delayed-type hypersensitivity (DTH) reaction in mice. Among the three major triterpene constituents isolated (i.e., alisol B, alisol B 23-acetate, alisol C 23-acetate) as active principles, alisol B and its 23-acetate strongly and significantly inhibited LT production and β-hexosaminidase release between 1-10 µM. On the other hand, all these alisol derivatives significantly and strongly inhibited DTH response after oral administration. In addition, AOE (200 mg/kg/d) was for the first time found to considerably alleviate hapten-induced dermatitis symptoms in NC/Nga mice, an animal model of atopic dermatitis. These results indicate that alisol derivatives possess inhibitory activities on immediate-type as well as delayed-type hypersensitivity reactions and may contribute to the anti-allergic action of AOE.
authors.affiliation: College of Pharmacy, Kangwon National University, Chunchon, Republic of Korea.
authors.forename: Je Hyeong
authors.initials: JH
authors.lastname: Lee
authors.forename: Oh Song
authors.initials: OS
authors.lastname: Kwon
authors.forename: Hong-Guang
authors.initials: HG
authors.lastname: Jin
authors.forename: Eun-Rhan
authors.initials: ER
authors.lastname: Woo
authors.forename: Yeong Shik
authors.initials: YS
authors.lastname: Kim
authors.forename: Hyun Pyo
authors.initials: HP
authors.lastname: Kim
mesh_terms: D034902:Alisma / Q000737:chemistry*; D000818:Animals; D000941:Antigens / Q000378:metabolism; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism; D045744:Cell Line, Tumor; D002783:Cholestenones / Q000494:pharmacology / Q000627:therapeutic use*; D003876:Dermatitis, Atopic / Q000378:metabolism / Q000517:prevention & control*; D004195:Disease Models, Animal; D006241:Haptens / Q000378:metabolism; D006968:Hypersensitivity, Delayed / Q000378:metabolism / Q000517:prevention & control*; D007938:Leukemia; D015289:Leukotrienes / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D027343:Rhizome / Q000737:chemistry; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D000941:Antigens; D002783:Cholestenones; D006241:Haptens; D015289:Leukotrienes; D010936:Plant Extracts; C526957:alisol B 23-acetate; C457232:alisol B; D001094:Arachidonate 5-Lipoxygenase; D001619:beta-N-Acetylhexosaminidases