title: [Protective effect of SjC23-Hsp70 DNA vaccine and interleukin-12 on Schistosoma japonicum infection in water buffalos].
abstract: OBJECTIVE
  To determine the immune-protective effect of Japan Schistosoma (Chinese mainland strain) 23 kD membrane protein-heat shock protein (SjC23-Hsp70) DNA vaccine plus adjuvantinduced interleukin-12 (IL-12) plasmid DNA on Schistosoma japonicum infection in water buffalos.
  
  
  METHODS
  Forty-five health water buffalos (8-10 months old) in non-endemic area of schistosomiasis were randomly assigned into group A (SjC23-Hsp70+IL-12, 300 μg), group B (SjC23+IL-12, 300 μg) and group C (pVAX+IL-12, 300 μg), 15 in each group. Each buffalo was immuned by shoulder intramuscular injection for 3 times, at an interval of 28 days. Twenty-eight days after the last immunization, each buffalo was infected with 1000 Japan cercariae of Schistosoma. Fecal examinations were conducted 2 days and 1 day before the perfusion, and on the day of perfusion. The number of hatching miracidia and eggs per gram feces was recorded. Fifty-six days after the infection, the buffalos were sacrificed and perfused via the descending aorta. The recovered adult worms and eggs in the liver tissue were counted.
  
  
  RESULTS
  We compared group A and B with group C: the estrogen reduction rate was 45.7% and 26.61%; bug reduction rate was 44.51% and 25.84%; the fecal egg reduction rate was 41.1% and 31.63%; the miracidium reduction rate was 48.11% and 38.07%; and the liver egg reduction rate was 43.39% and 31.95%. The above rates in group A were higher than those in group B (P<0.05).
  
  
  CONCLUSIONS
  SjC23-Hsp70 DNA vaccine combined with IL-12 may have a significant immunoprotective effect on buffalos.
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Central South University, Changsha 410078,China.
authors.forename: Pingcheng
authors.initials: P
authors.lastname: Hu
authors.forename: Da
authors.initials: D
authors.lastname: Xia
authors.forename: Hongyan
authors.initials: H
authors.lastname: Cui
authors.forename: Pingfang
authors.initials: P
authors.lastname: Zhang
authors.forename: Yongkang
authors.initials: Y
authors.lastname: He
authors.forename: Xinling
authors.initials: X
authors.lastname: Yu
authors.forename: Zhenqiu
authors.initials: Z
authors.lastname: Sun
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D002020:Buffaloes; D002417:Cattle; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D015801:Helminth Proteins / Q000276:immunology*; D007114:Immunization / Q000379:methods; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D018840:HSP70 Heat-Shock Proteins; D015801:Helminth Proteins; D008565:Membrane Proteins; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; C072633:Sj23 antigen, Schistosoma japonicum; D018664:Interleukin-12