title: Development of poly(vinyl acetate-methylacrylic acid)/chitosan/Fe3O4 nanoparticles for the diagnosis of non-alcoholic steatohepatitis with magnetic resonance imaging.
abstract: Non-alcoholic steatohepatitis is a burgeoning health problem. To diagnose NASH with magnetic resonance imaging (MRI), an effective contrast agent, a stable suspension of superparamagnetic Fe(3)O(4) nanoparticles, were newly developed. The negatively charged Fe(3)O(4) nanoparticles were coated with positive chitosan (CS) firstly, and then assembled with poly(vinyl acetate-methylacrylic acid) (P(VAc-MAA)). Transmission electron microscope and dynamic light scattering confirmed that the obtained P(VAc-MAA)/CS/Fe(3)O(4) nanoparticles had a spherical or ellipsoidal morphology with an average diameter in the range of 14-20 nm. The superparamagnetic property and spinel structure of the Fe(3)O(4) nanoparticles were well preserved due to the protection of the P(VAc-MAA)/CS layers on the surface of the Fe(3)O(4) nanoparticles. The in vivo rat experiments confirmed that the P(VAc-MAA)/CS/Fe(3)O(4) nanoparticles were an effective contrast agent for MRI to diagnose NASH.
authors.affiliation: College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, People's Republic of China.
authors.forename: Xiadan
authors.initials: X
authors.lastname: Luo
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Song
authors.forename: Aiping
authors.initials: A
authors.lastname: Zhu
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Si
authors.forename: Lijun
authors.initials: L
authors.lastname: Ji
authors.forename: Zhanrong
authors.initials: Z
authors.lastname: Ma
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Jiao
authors.forename: Jingtao
authors.initials: J
authors.lastname: Wu
mesh_terms: D000179:Acrylates / Q000737:chemistry*; D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D003287:Contrast Media / Q000494:pharmacology; D005234:Fatty Liver / Q000378:metabolism*; D005290:Ferric Compounds / Q000737:chemistry*; D008027:Light; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014753:Vinyl Compounds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000179:Acrylates; D003287:Contrast Media; D005290:Ferric Compounds; D014753:Vinyl Compounds; C000499:ferric oxide; D048271:Chitosan; C036658:acrylic acid; C011566:vinyl acetate