title: Ligand-incorporation site in 5-methylcytosine-detection probe modulating the site of osmium complexation with the target DNA.
abstract: ICON Probes, short DNA strands containing an adenine linked to a bipyridine ligand, formed an interstrand cross-link with 5-methylcytosine located opposite the modified adenine in the presence of an osmium oxidant. The location of a bipyridine-tethered adenine in the probes varied the selectivity of the reactive base. An ICON probe where the modified adenine was located at the probe center showed a 5-methylcytosine-selective osmium complexation, whereas an ICON probe with the modified adenine at the strand end exhibited high reactivity towards thymine as well as 5-methylcytosine. The modulation of reactive bases by the incorporation of a bipyridine-tethered adenine site made facilitates design of ICON probes for the fluorometric detection of 5-methylcytosine.
authors.affiliation: Nucleic Acid Chemistry Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Sugizaki
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakamura
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yanagisawa
authors.forename: Akimitsu
authors.initials: A
authors.lastname: Okamoto
mesh_terms: D044503:5-Methylcytosine / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D016503:Drug Delivery Systems; D005453:Fluorescence; D008024:Ligands; D015394:Molecular Structure; D009992:Osmium / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; D008024:Ligands; D009992:Osmium; D044503:5-Methylcytosine; D004247:DNA