title: Icaritin, an exogenous phytomolecule, enhances osteogenesis but not angiogenesis--an in vitro efficacy study.
abstract: We found that Icaritin, an intestinal metabolite of Epimedium-derived flavonoids (EF) enhanced osteoblastic differentiation of mesenchymal stem cells (MSCs) only under osteogenic induction conditions. We also demonstrated its effect on inhibition of adipogenic differentiation of MSCs. Unlike the findings of others on EF compounds, we showed that Icaritin was unable to promote proliferation, migration and tube like structure formation by human umbilical vein endothelial cells (HUVECs) in vitro. These results suggested that the exogenous phytomolecule Icaritin possessed the potential for enhancing bone formation via its osteopromotive but not an osteoinductive mechanism. Though some flavonoids were shown to regulate the coupling process of angiogenesis and osteogenesis during bone repair, our results suggested that Icaritin did not have direct effect on enhancing angiogenesis in vitro.
authors.affiliation: Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Dong
authors.initials: D
authors.lastname: Yao
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Xie
authors.forename: Xin-Luan
authors.initials: XL
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Wan
authors.forename: Yuk-Wai
authors.initials: YW
authors.lastname: Lee
authors.forename: Shi-Hui
authors.initials: SH
authors.lastname: Chen
authors.forename: Duan-Qing
authors.initials: DQ
authors.lastname: Pei
authors.forename: Yi-Xiang
authors.initials: YX
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology; D001842:Bone and Bones / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D004354:Drug Screening Assays, Antitumor; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D005419:Flavonoids / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D009389:Neovascularization, Pathologic; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis / Q000502:physiology*; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; C499403:icaritin