title: Novel analysis model for implant osseointegration using ectopic bone formation via the recombinant human bone morphogenetic protein-2/macroporous biphasic calcium phosphate block system in rats: a proof-of-concept study.
abstract: OBJECTIVE
  The osseointegration around titanium mini-implants installed in macroporous biphasic calcium phosphate (MBCP) blocks was evaluated after incubation with recombinant human bone morphogenetic protein-2 (rhBMP-2) in an ectopic subcutaneous rat model.
  
  
  METHODS
  Mini-implants (φ1.8×12 mm) were installed in MBCP blocks (bMBCPs, 4×5×15 mm) loaded with rhBMP-2 at 0.1 mg/mL, and then implanted for 8 weeks into subcutaneous pockets of male Sprague-Dawley rats (n=10). A histomorphometric analysis was performed, and the bone-to-implant contact (BIC) and bone density were evaluated.
  
  
  RESULTS
  Significant osteoinductive activity was induced in the rhBMP-2/bMBCP group. The percentage of BIC was 41.23±4.13% (mean±standard deviation), while bone density was 33.47±5.73%. In contrast, no bone formation was observed in the bMBCP only group.
  
  
  CONCLUSIONS
  This model represents a more standardized tool for analyzing osseointegration and bone healing along the implant surface and in bMBCPs that excludes various healing factors derived from selected animals and defect models.
authors.affiliation: Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry, Seoul, Korea.
authors.forename: Jung-Chul
authors.initials: JC
authors.lastname: Park
authors.forename: Jong-Bin
authors.initials: JB
authors.lastname: Lee
authors.forename: Guy
authors.initials: G
authors.lastname: Daculsi
authors.forename: Sang-Yeop
authors.initials: SY
authors.lastname: Oh
authors.forename: Kyoo-Sung
authors.initials: KS
authors.lastname: Cho
authors.forename: Gun-Il
authors.initials: GI
authors.lastname: Im
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Chang-Sung
authors.initials: CS
authors.lastname: Kim