title: Smooth muscle and endothelial cell behaviour on degradable copolyetheresterurethane films.
abstract: Stent thrombosis and restenosis after drug-eluting stent (DES) implantation remains a relevant problem in the cardiovascular field. The polymer-based biomaterial (e.g. stent coating) requirements are comprehensive, since the polymeric material ideally should ensure an effective re-endothelialization by recruiting endothelial cells (EC) and endothelial progenitor cells (EPC). Simultaneously, the polymer should effectively prevent adherence of smooth muscle cells (SMC) and thereby inhibiting restenosis. The aim of this study was to gain a basic understanding on the interaction of SMC and human umbilical vein endothelial cells (HUVEC) with nonporous polymer films. A multifunctional copolyetheresterurethane (PDC) was chosen as candidate material: PDC consists of poly(p-dioxanone) (PPDO) and poly(ε-caprolactone)-segments (PCL). In our study it was compared to the degradable PPDO homopolymer and poly(vinylidene fluoride-co-hexafluoropropene) (PVDF), an established coating material of DES in clinical applications intended for longterm applications. The films were analyzed according to their thermomechanical and surface properties before being examined in contact with HUVEC and SMC concerning cell viability, proliferation and adhesion. Experimental results showed that adhesion could be improved for HUVEC on PDC compared to PPDO and PVDF. In contrast, SMC attachment is largely suppressed on PDC polymeric films indicating a cell-specific response of HUVEC towards PDC. In conclusion, PDC represents a promising candidate material for future cardiovascular applications like e.g. biodegradable (PDC) stent coatings.
authors.affiliation: Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany. constantin.rueder@charite.de
authors.forename: Constantin
authors.initials: C
authors.lastname: Rüder
authors.forename: Tilman
authors.initials: T
authors.lastname: Sauter
authors.forename: Karl
authors.initials: K
authors.lastname: Kratz
authors.forename: Jan
authors.initials: J
authors.lastname: Peter
authors.forename: Friedrich
authors.initials: F
authors.lastname: Jung
authors.forename: Andreas
authors.initials: A
authors.lastname: Lendlein
authors.forename: Dietlind
authors.initials: D
authors.lastname: Zohlnhöfer
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods; D004146:Dioxanes; D054855:Drug-Eluting Stents; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009130:Muscle, Smooth / Q000166:cytology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011091:Polyesters; D011108:Polymers; D011140:Polyurethanes; D011145:Polyvinyls
chemical_list: D001672:Biocompatible Materials; D004146:Dioxanes; D011091:Polyesters; D011108:Polymers; D011140:Polyurethanes; D011145:Polyvinyls; C545920:poly(vinylidene fluoride-co-hexafluoro propylene); C016240:polycaprolactone; C079733:poly-4-dioxan-2-one