title: CUG binding protein 1 binds to a specific region within the human albumin 3' untranslated region.
abstract: 3' Untranslated regions (3'UTRs) of messenger RNAs have important roles in post-transcriptional regulation of gene expression and this is partly achieved through binding of specific proteins to sequences or structures within these regions. Previously, replacement of a native luciferase 3'UTR with the human albumin 3'UTR has been found to lead to a 10-fold increase in luciferase reporter activity. In this work we investigated protein binding to the human albumin 3'UTR. Electrophoretic mobility shift and UV cross-linking assays indicate that a ∼50kDa protein from Chinese Hamster Ovary (CHO) cells binds to the albumin 3'UTR, and affinity experiments followed by proteomics identified this protein as CUG binding protein 1 (CUG-BP1, also known as CELF1). Deletion analysis of the albumin 3'UTR showed that nucleotides 1-50 and nucleotides 101-150 are not required for binding but that removal of nucleotides 51-100 caused a loss in binding. The results suggest that CUG-BP1 binds to nucleotides 51-100 of the human albumin 3'UTR. In human cells CUG-BP1 binding may thus play a role in regulation of albumin expression and, additionally, it may have a function in post-transcriptional control in CHO cells.
authors.affiliation: Institute for Cell and Molecular Biosciences, The Medical School, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4HH, UK.
authors.forename: Siavash
authors.initials: S
authors.lastname: Khaziapoul
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Pearson
authors.forename: Ian F
authors.initials: IF
authors.lastname: Pryme
authors.forename: Beate
authors.initials: B
authors.lastname: Stern
authors.forename: John E
authors.initials: JE
authors.lastname: Hesketh
mesh_terms: D020413:3' Untranslated Regions; D000418:Albumins / Q000235:genetics*; D000818:Animals; D000067879:CELF1 Protein; D016466:CHO Cells; D006224:Cricetinae; D024202:Electrophoretic Mobility Shift Assay; D005786:Gene Expression Regulation; D006801:Humans; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D000418:Albumins; D000067879:CELF1 Protein; C102843:CELF1 protein, human; D016601:RNA-Binding Proteins