title: Antioxidant and anti-cholinesterase activity of Globularia meridionalis extracts and isolated constituents.
abstract: The antioxidant and anti-cholinesterase properties of Globularia meridionalis (Podp.) O. Schwarz (Globulariaceae) were investigated. G. meridionalis aerial parts and roots were extracted with methanol, and the total extract successively partitioned with n-hexane. The methanol fraction of the roots showed the highest antioxidant activity with IC50 values of 8.0 and 2.1 microg/mL in the DPPH and beta-carotene bleaching tests, respectively. From this fraction, three flavonoids, namely pectolinarigenin 7-O-beta-glucoside, apigenin 7-O-beta-glucoside and luteolin, and two phenylethanoids, namely acteoside and isoacteoside, were isolated. Potential anti-cholinesterase effects were assessed through the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes inhibition assay. The n-hexane fraction of the roots exhibited the highest activity against both AChE and BChE with IC50 values of 65.5 and 70.4 microg/mL, respectively.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy and Nutrition and Health Sciences, University of Calabria, I-87030 Rende (CS), Italy. tundis@unical.it
authors.forename: Rosa
authors.initials: R
authors.lastname: Tundis
authors.forename: Marco
authors.initials: M
authors.lastname: Bonesi
authors.forename: Federica
authors.initials: F
authors.lastname: Menichini
authors.forename: Monica R
authors.initials: MR
authors.lastname: Loizzo
authors.forename: Filomena
authors.initials: F
authors.lastname: Conforti
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Statti
authors.forename: Filippo M
authors.initials: FM
authors.lastname: Pirisi
authors.forename: Francesco
authors.initials: F
authors.lastname: Menichini
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D002091:Butyrylcholinesterase / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D028083:Scrophulariaceae / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D002091:Butyrylcholinesterase