title: Overexpression of neutrophil gelatinase-associated lipocalin and its receptor in colorectal carcinoma: Significant correlation with cell differentiation and tumour invasion.
abstract: Neutrophil gelatinase-associated lipocalin (NGAL), a member of the lipocalin family, is related to imflammation and tumour. Recently, a specific cell-surface receptor (24p3R/NGALR) for lipocalin 24p3 was reported. However, the characteristics of NGALR expression in colorectal carcinoma (CRC) are not known. The objectives of this study were to investigate the expression of NGAL and NGALR in CRC specimens, and determine any relationship between the expression of these proteins and tumour progression. In the present study, CRC specimens of 102 patients were obtained, and the expression of NGAL, NGALR, ferritin and Ki67 was analyzed in paraffin sections by immunohistochemistry. Statistical analyses of the data collected were performed with SPSS software. We found that the cytoplasmic staining of NGAL, NGALR and ferritin, as well as the nuclear staining of Ki67 were significantly up-regulated in CRC tissues compared with normal colorectal tissues. Expression of NGAL was related to the deeper invasion of CRC (P=0.026), while NGALR was significantly associated with a deeper invasion (P=0.018) and a high degree of Tumor, Node and Metastasis stages (P=0.042) in CRC. The NGAL/NGALR co-expression was associated with poor cellular differentiation (P=0.004). Positive correlations between NGAL and NGALR (r=0.432, P<0.01), NGAL and ferritin (r=0.374, P<0.001), NGALR and Ki67 (r=0.228, P<0.05), NGAL/NGALR co-expression and ferritin (r=0.349, P<0.001), as well as NGAL/NGALR co-expression and Ki67 (r=0.205, P<0.05) were observed. However, the expression of NGAL or NGALR was not significantly associated with patient survival. These findings detected an elevated expression of NGAL and NGALR resulting in poor cellular differentiation and a deeper invasion of CRC. Thus, NGALR may be a novel target for the treatment of CRC.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Shantou University, Shantou 515041, P.R. China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Lv
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xu
authors.forename: Zhong-Ying
authors.initials: ZY
authors.lastname: Shen
authors.forename: Fa-Ren
authors.initials: FR
authors.lastname: Zhang
authors.forename: Xiu-E
authors.initials: XE
authors.lastname: Xu
authors.forename: En-Min
authors.initials: EM
authors.lastname: Li