title: [Screening and expression of CD34(+) cell-specific microRNA in acute myelogenous leukemia].
abstract: OBJECTIVE
  To screen and analyze CD34(+) cell specific microRNAs (miRNAs) from the patients with acute myelogenous leukemia (AML) and their expression.
  
  
  METHODS
  CD34(+) cells were sorted from AML patients or the mobilized peripheral blood of the donors of hematopoietic stem cell transplantation (normal control subjects) and followed by the extraction of the cell total RNAs. The differentially expressed microRNAs (miRNAs, miR) were selected after hybridizing with miRNA microarray, real time polymerase chain reaction (real-time PCR) was subsequently applied to confirm the expression of the selected miRs, and PCR products were further cloned and sequenced to check their specificity.
  
  
  RESULTS
  Of the differentially expressed miRNAs, 191 were found to be at least one-fold change in the CD34(+) cells between the AML patients and the normal control subjects. Of the 191 miRNAs, the expression difference of 94 was significant (P < 0.05). Among these 94 miRNAs, the expression of 44 miRNAs was increased and the other 50 miRNAs was decreased in the CD34(+) cells from the bone marrow of AML patients compared with the CD34(+) cells from the mobilized peripheral blood of the normal control subjects. Real time PCR verified that the expression level of miR-10a and miR-220c in the CD34(+) cells from the bone marrow of AML patients was 19.6% and 19.0% of that of CD34(+) cells from mobilized peripheral blood of the normal control subjects. DNA sequencing and BLAST DNA database searching results indicated that the PCR products were really miR-10a and miR-220c.
  
  
  CONCLUSIONS
  A variety of differentially expressed-miRNAs are existed between AML and normal control subjects CD34(+) cells, the expression of miR-10a and miR-220c was significantly down-regulated in the CD34(+) cells from the bone marrow of AML patients.
authors.affiliation: Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China.
authors.forename: Guang-ping
authors.initials: GP
authors.lastname: Wang
authors.forename: Shu-qin
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.forename: Min-yuan
authors.initials: MY
authors.lastname: Peng
authors.forename: San-qin
authors.initials: SQ
authors.lastname: Tan
authors.forename: Hui
authors.initials: H
authors.lastname: Yin
authors.forename: Ya-jing
authors.initials: YJ
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Fang-ping
authors.initials: FP
authors.lastname: Chen
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D005260:Female; D006412:Hematopoietic Stem Cells; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D018952:Antigens, CD34; D035683:MicroRNAs