title: Determination of nonmammalian ghrelin.
abstract: Ghrelin is a peptide with a unique molecular modification by a fatty acid such as n-octanoic acid. The acyl modification is necessary for ghrelin to bind to its receptor (growth hormone secretagogue-receptor 1a, GHS-R1a) and to induce subsequent intracellular Ca(2+) signaling. Ghrelin is widely expressed in mammals as well as in nonmammalian vertebrates. In our laboratory, a method for efficient purification of ghrelin from a small amount of tissues has been established. Here, we introduce the identification process of ghrelin in nonmammalian vertebrates.
authors.affiliation: Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, Japan. kaiya@ri.ncvc.go.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kaiya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hosoda
authors.forename: Kenji
authors.initials: K
authors.lastname: Kangawa
authors.forename: Mikiya
authors.initials: M
authors.lastname: Miyazato
mesh_terms: D000595:Amino Acid Sequence; D000663:Amphibians / Q000378:metabolism; D000818:Animals; D001717:Birds / Q000378:metabolism; D020013:Calcium Signaling; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005399:Fishes / Q000378:metabolism*; D054439:Ghrelin / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D051381:Rats; D054440:Receptors, Ghrelin / Q000235:genetics / Q000378:metabolism*; D012104:Reptiles / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D054439:Ghrelin; D007074:Immunoglobulin G; D054440:Receptors, Ghrelin