title: [Screening of membrane antigen differentially expressed in androgen-dependent prostate cancer and androgen-independent prostate cancer].
abstract: OBJECTIVE
  To determine the screening of the expression of membrane proteins in androgen-dependent prostate cancer (ADPC) and androgen-independent prostate cancer (AIPC) and to explore the mechanism of membrane proteins in these two cancers.
  
  
  METHODS
  Serum samples were collected from 3 patients with ADPC and another 3 patients with AIPC. The serum was incubated with ADPC cell line LNCaP and/or AIPC cell line PC-3 and detected by immunoprecipitation and Western blot. Differentially expressed proteins between ADPC and AIPC identified by mass spectrometry were compared and their expression level and location were analyzed by immunofluorescence.
  
  
  RESULTS
  Altogether 11 membrane proteins were identifited, such as the Neural-Cadherin precursor, ER60 precursor, Claudin-4, and so on. Immunofluorescence revealed that the expression level of Claudin-4 in PC-3 cells was higher than in LNCaP cells.
  
  
  CONCLUSIONS
  We can use the screening method to study membrane proteins in prostate cancer. Claudin-4 may play an important role in the pathogenesis and the development of AIPC.
authors.affiliation: Department of Geriatric Urology, Xiangya Hospital, Central South University, Changsha 410008, China. xiaobozxb@yahoo.com.cn
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Tang
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.forename: Hequn
authors.initials: H
authors.lastname: Chen
authors.forename: Qizhan
authors.initials: Q
authors.lastname: Luo
mesh_terms: D000368:Aged; D000728:Androgens / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D062506:Claudin-4 / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D019704:Protein Disulfide-Isomerases / Q000235:genetics / Q000378:metabolism
chemical_list: D000728:Androgens; C568157:CLDN4 protein, human; D062506:Claudin-4; D008562:Membrane Glycoproteins; D019704:Protein Disulfide-Isomerases; C085868:PDIA3 protein, human