title: [Establishing a high-titer infectious avian influenza A (H5N1) pseudotyped viral particle].
abstract: A transient four-plasmid cotransfection system was used to construct avian influenza A (H5N1) pseudotyped viral particle (H5N1Pp) by incorporating hemagglutinin (HA) protein and neuraminidase (NA) protein from H5N1 avian influenza virus onto Murine leukemia virus pseudotyped viral particles, the transmission electron microscopy, infectivity titer assay, hemagglutination assay, neutralization assay of H5N1Pp were studied. We established a pseudotyped H5N1 viral particle at a high titer of 10(8) Pp/mL, the morphology,the hemagglutination activity and neutralization specificity of H5N1Pp is simililar to wild H5N1 virus. The research result sets a platform for studying this virus, including its receptors, the functional analysis of HA and NA, neutralizing antibodies and anti-H5N1 drug development.
authors.affiliation: Institute of Medical Virology, School of Laboratory Medicine and Life Science, Wenzhou Medical College, Wenzhou 325035, China. liuxijun.jack@126.com
authors.forename: Xi-Jun
authors.initials: XJ
authors.lastname: Liu
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Guo
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Shen
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Shi
authors.forename: Guo-Hui
authors.initials: GH
authors.lastname: Zhang
authors.forename: Xun
authors.initials: X
authors.lastname: Zhang
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Bi
authors.forename: Hong-Lan
authors.initials: HL
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001717:Birds; D006224:Cricetinae; D005818:Genetic Engineering; D057809:HEK293 Cells; D006384:Hemagglutination; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics* / Q000502:physiology*; D005585:Influenza in Birds / Q000821:virology*; D009500:Neutralization Tests; D014162:Transfection; D019562:Viral Load / Q000235:genetics*; D014771:Virion / Q000235:genetics*
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus