title: Determination of cathepsin V activity and intracellular trafficking by N-glycosylation.
abstract: Cathepsin V (L2), a lysosomal cysteine protease, is a member of cathepsin family, relating to cancer invasion and metastasis. Cathepsin V contains two predicted N-glycosylation sites, but it has not been reported whether cathepsin V is glycosylated or not. In this study, we clarified the role of N-glycosylation of cathepsin V for its functions. We demonstrated that cathepsin V is N-glycosylated at both Asn(221) and Asn(292) using mass spectrometry and site-directed mutagenesis. N-glycosylation of cathepsin V was important for transportation to lysosome, secretion, and activity in HT1080 cells. These data demonstrated that functions of cathepsin V are controlled by N-glycosylation.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Niwa
authors.forename: Takehiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.forename: Kazuo
authors.initials: K
authors.lastname: Umezawa
authors.forename: Siro
authors.initials: S
authors.lastname: Simizu
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002403:Cathepsins / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008969:Molecular Sequence Data; D021381:Protein Transport
chemical_list: D002403:Cathepsins; D003546:Cysteine Endopeptidases; C117627:CTSV protein, human