title: Expulsion of selenium/protein nanoparticles through vesicle-like structures by Saccharomyces cerevisiae under microaerophilic environment.
abstract: Nano-selenium/protein is a kind of lower toxic supplement to human. Many microorganisms can reduce selenite/selenate to intracellular or extracellular selenium nanoparticles. This study examined the influence of dissolved oxygen on the expulsion of extracellular selenium/protein produced in Saccharomyces cerevisiae. More of the added selenite was reduced to extracellular selenium nanoparticles by yeast cells only under oxygen-limited condition than under aerobic or anaerobic condition. For the first time, we evidenced that selenium/protein nanoparticles synthesized in vivo were transported out of the cells by vesicle-like structures under microaerophilic environment. The characterizations of the extracellular spherical selenium/protein nanoparticles were also examined by SEM, TEM, EDX and FTIR.
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, No.9 Section 4, Renmin Nan Road, Chengdu, 610041, Sichuan, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Daping
authors.initials: D
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Gao
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D055354:Exosomes / Q000378:metabolism*; D005583:Fourier Analysis; D006801:Humans; D008854:Microscopy, Electron; D053758:Nanoparticles / Q000648:ultrastructure*; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D012643:Selenium