title: Assay of tyrosol and hydroxytyrosol in olive oil by tandem mass spectrometry and isotope dilution method.
abstract: Hydroxytyrosol and tyrosol, the strong antioxidant present in large amount in virgin olive oil have been assayed by LC-MS/MS under MRM condition and isotope dilution method, using d(2)-labelled internal standards obtained by simple synthetic procedures. The assay has been performed under MRM condition monitoring two transitions for each analyte to improve the specificity. This paper deals with a modern approach for assaying the content of this polyphenols in virgin olive oil down to a limit of a few hundreds of parts per billion. Tyrosol and hydroxytyrosol ranged from 10 to 47ppm and from 5 to 25ppm in commercial olive oil, respectively. The accuracy (98-107%) and analytical parameters values confirm the reliability of the proposed approach. The method can be extended to any natural matrices, including mill wastes, after a simple step of sample preparation.
authors.affiliation: Dipartimento di Chimica, Università della Calabria, cubo 12/C, I-87036 Arcavacata di Rende, CS, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Mazzotti
authors.forename: Hicham
authors.initials: H
authors.lastname: Benabdelkamel
authors.forename: Leonardo
authors.initials: L
authors.lastname: Di Donna
authors.forename: Loredana
authors.initials: L
authors.lastname: Maiuolo
authors.forename: Anna
authors.initials: A
authors.lastname: Napoli
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sindona
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D007553:Isotope Labeling / Q000379:methods*; D000069463:Olive Oil; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000975:Antioxidants; D000069463:Olive Oil; D010938:Plant Oils; C005975:3,4-dihydroxyphenylethanol; C011867:4-hydroxyphenylethanol; D010626:Phenylethyl Alcohol