title: The microtubule associated protein syntabulin is required for glucose-stimulated and cAMP-potentiated insulin secretion.
abstract: Syntabulin is a microtubule-associated protein that mediates anterograde transport of vesicles to neuronal processes. Here, we found that syntabulin was expressed in mouse pancreas and insulin-secreting β-cells, and that it partially co-localized with microtubule and insulin-containing granules. The association of syntabulin with these organelles increased upon glucose stimulation. Knock-down of syntabulin by shRNA reduced both basal and glucose-stimulated insulin secretion, and diminished cAMP-Epac2 and cAMP-PKA potentiated insulin secretion. Additionally, syntabulin was preferentially phosphorylated by the Epac2 agonist 8-pCPT-2'-O-Me-cAMP, suggesting that syntabulin could be a novel effector of Epac2 and play a critical role in cAMP-enhanced insulin secretion.
authors.affiliation: Dept. of Physiology, School of Medicine, Shenzhen University, Shenzhen 518060, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ying
authors.forename: Lingyun
authors.initials: L
authors.lastname: Li
authors.forename: Wen
authors.initials: W
authors.lastname: Cao
authors.forename: Dan
authors.initials: D
authors.lastname: Yan
authors.forename: Qing
authors.initials: Q
authors.lastname: Zeng
authors.forename: Xiangchen
authors.initials: X
authors.lastname: Kong
authors.forename: Lina
authors.initials: L
authors.lastname: Lu
authors.forename: Meng
authors.initials: M
authors.lastname: Yan
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Xu
authors.forename: Junle
authors.initials: J
authors.lastname: Qu
authors.forename: Qingning
authors.initials: Q
authors.lastname: Su
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D005947:Glucose / Q000494:pharmacology*; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D021381:Protein Transport; D051381:Rats; D009435:Synaptic Transmission
chemical_list: D020662:Guanine Nucleotide Exchange Factors; D007328:Insulin; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; C486707:Rapgef4 protein, mouse; C464570:SYBU protein, human; D000242:Cyclic AMP; D005947:Glucose