title: Differential investigations from plasma-derived and recombinant Factor IX revealed major differences in post-translational modifications of activation peptides.
abstract: Post-translational modifications (PTMs) located on the activation peptide (AP) of recombinant FIX (rFIX, BeneFIX(®) ) and plasma-derived FIX (pdFIX, Betafact(®) ) have been investigated by mass spectrometry to review the structural differences between these two products. Three major structural differences were pointed out. rFIX contains a low amount of phosphorylated and sulphated AP (4% for rFIX vs. 70% for pdFIX); rFIX N-glycans are only sialylated in the α2-3 linkage, whereas pdFIX N-glycans contain both type of α2-3 and α2-6 linkages, and rFIX does not contain any sialyl Lewis(X)  glycoantigens contrary to pdFIX. These variations might participate in the in vivo potential different behaviours of the two molecules.
authors.affiliation: LFB Biotechnologies, Courtaboeuf Cedex, France LFB Biomédicaments, Courtaboeuf Cedex, France. chevreuxg@lfb.fr
authors.forename: G
authors.initials: G
authors.lastname: Chevreux
authors.forename: V
authors.initials: V
authors.lastname: Faid
authors.forename: M-H
authors.initials: MH
authors.lastname: Andre
authors.forename: Z
authors.initials: Z
authors.lastname: Tellier
authors.forename: N
authors.initials: N
authors.lastname: Bihoreau
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D005164:Factor IX / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D010446:Peptide Fragments / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010446:Peptide Fragments; D011994:Recombinant Proteins; D005164:Factor IX