title: A reaction-based fluorescent probe for selective imaging of carbon monoxide in living cells using a palladium-mediated carbonylation.
abstract: Carbon monoxide is a member of the gasotransmitter family, which also includes NO and H(2)S, and has been implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well-studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. Here we report a new type of turn-on fluorescent probe for selective CO detection based on palladium-mediated carbonylation reactivity. CO Probe 1 (COP-1) is capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.
authors.affiliation: Department of Chemistry, University of California, Berkeley, California 94720, United States.
authors.forename: Brian W
authors.initials: BW
authors.lastname: Michel
authors.forename: Alexander R
authors.initials: AR
authors.lastname: Lippert
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Chang
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D002248:Carbon Monoxide / Q000032:analysis*; D056831:Coordination Complexes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal; D010165:Palladium / Q000737:chemistry*
chemical_list: D001896:Boron Compounds; C000597172:CO probe 1; D056831:Coordination Complexes; D005456:Fluorescent Dyes; D010165:Palladium; D002248:Carbon Monoxide