title: Effect of the ketamine/xylazine anesthetic on the auditory brainstem response of adult gerbils.
abstract: The auditory brainstem response (ABR) is a test widely used to assess the integrity of the brain stem. Although it is considered to be an auditory-evoked potential that is influenced by the physical characteristics of the stimulus, such as rate, polarity and type of stimulus, it may also be influenced by the change in several parameters. The use of anesthetics may adversely influence the value of the ABR wave latency. One of the anesthetics used for e ABR assessment, especially in animal research, is the ketamine/xylazine combination. Our objective was to determine the influence of the ketamine/xylazine anesthetic on the ABR latency values in adult gerbils. The ABRs of 12 adult gerbils injected with the anesthetic were collected on three consecutive days, or a total of six collections, namely: pre-collection and A, B, C, D, and E collections. Before each collection the gerbil was injected with a dose of ketamine (100 mg/kg)/xylazine (4 mg/kg). For the capture of the ABR, 2000 click stimuli were used with rarefaction polarity and 13 stimuli per second, 80 dBnHL intensity and in-ear phones. A statistically significant difference was observed in the latency of the V wave in the ABR of gerbils in the C and D collections compared to the pre-, A and E collections, and no difference was observed between the pre-, A, B, and E collections. We conclude that the use of ketamine/xylazine increases the latency of the V wave of the ABR after several doses injected into adult gerbils; thus clinicians should consider the use of this substance in the assessment of ABR.
authors.affiliation: Programa em Ciências da Reabilitação, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil. janapatricio@yahoo.com.br
authors.forename: J P
authors.initials: JP
authors.lastname: Lima
authors.forename: S
authors.initials: S
authors.lastname: Ariga
authors.forename: I
authors.initials: I
authors.lastname: Velasco
authors.forename: E
authors.initials: E
authors.lastname: Schochat
mesh_terms: D000777:Anesthetics / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects*; D005849:Gerbillinae; D007649:Ketamine / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D011930:Reaction Time; D014991:Xylazine / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000777:Anesthetics; D014991:Xylazine; D007649:Ketamine