title: Cloning, characterization and expression analysis of a cold shock domain family member YB-1 in turbot Scophthalmus maximus.
abstract: The Y-box proteins are a family of highly conserved nucleic acid binding proteins. In this report we have identified a new member, YB-1 from turbot (Scophthalmus maximus) spleen cDNA library. The full-length cDNA sequence of turbot YB-1 was obtained and then the expression at transcriptional level was researched by qRT-PCR. In normal organs, the expression of YB-1 was higher in liver, brain, gill and heart, respectively. YB-1 had the highest expression level at gastrula stage during the early stages of embryo development. In the liver, kidney and spleen, the turbot YB-1 expression level was the highest at 72 h after challenge with lymphocystis disease virus (LCDV) and the highest at 12 h after challenge with Vibrio anguillarum (V. anguillarum). Furthermore, the expression of turbot YB-1 also distinctly increased in turbot kidney cells (TK) at 24 h after challenge with V. anguillarum and LCDV. These results indicated that the turbot YB-1 protein may play a signiﬁcant role in the immune response of turbot.
authors.affiliation: Translational Center for Stem Cell Research, Tongji Hospital, Stem Cell Research Center, Tongji University School of Medicine, Shanghai 200065, China.
authors.forename: Xian-Li
authors.initials: XL
authors.lastname: Wang
authors.forename: Yu-Xi
authors.initials: YX
authors.lastname: Zhang
authors.forename: Chang-Geng
authors.initials: CG
authors.lastname: Yang
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Song-Lin
authors.initials: SL
authors.lastname: Chen
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D057773:Cold Shock Proteins and Peptides / Q000235:genetics* / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D004266:DNA Virus Infections / Q000276:immunology / Q000662:veterinary*; D018076:DNA, Complementary / Q000235:genetics; D005393:Fish Diseases / Q000196:embryology / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D005412:Flatfishes; D020869:Gene Expression Profiling / Q000662:veterinary; D018507:Gene Expression Regulation, Developmental / Q000276:immunology*; D007497:Iridoviridae / Q000276:immunology; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000378:metabolism; D014735:Vibrio Infections / Q000276:immunology / Q000662:veterinary*; D051840:Y-Box-Binding Protein 1 / Q000235:genetics* / Q000276:immunology
chemical_list: D057773:Cold Shock Proteins and Peptides; D017931:DNA Primers; D018076:DNA, Complementary; D051840:Y-Box-Binding Protein 1