title: Curtailing overexpression of E2F3 in breast cancer using siRNA (E2F3)-based gene silencing.
abstract: OBJECTIVE
  The E2F3 transcription factor claims its role in controlling cell cycle progression. As reported earlier, nuclear E2F3 overexpression leads to development of bladder and prostate cancer in humans. Accordingly, the present investigation has been designed to assess to what extent E2F3 would be overexpressed in breast cancer. The aim of this study was to emphasize that the levels of E2F3 are increased in breast cancer and highlights the efficacy of siRNA targeted to E2F3.
  
  
  METHODS
  To investigate the expression level of E2F3 and the progression of breast tumors, quantitative real-time PCR analysis was carried out. Western blotting analysis was performed to measure its counterparts, namely, E2F3a and E2F3b.
  
  
  RESULTS
  In the novel axis of E2F3, a large set of 11 breast cancer cell lines were identified to have the property of overexpression. Furthermore, the small interfering RNA (siRNA) developed against E2F3 significantly blocked the expression of the E2F3 in the selected breast cancer cell lines. Thus, the present findings authenticate the efficiency of siRNA (E2F3) to fight against breast cancer; hence, the siRNA mediated E2F3 gene silencing knockdown the E2F3.
  
  
  CONCLUSIONS
  This in vitro study demonstrates that E2F3 is a newly identified diagnostic and potential therapeutic target in breast cancer. Outcomes of this study affirm that siRNA for E2F3 facilitates the silencing of E2F3 overexpression and fights against breast cancer. Therefore, it plays a vital role as an alternative for diagnosis and clinical outcome for the treatment of breast cancer.
authors.affiliation: Proteomics and Molecular Cell Physiology Laboratory, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, TN 641046, India.
authors.forename: Karuppaiya
authors.initials: K
authors.lastname: Vimala
authors.forename: Shenbagamoorthy
authors.initials: S
authors.lastname: Sundarraj
authors.forename: Mohanan V
authors.initials: MV
authors.lastname: Sujitha
authors.forename: Soundarapandian
authors.initials: S
authors.lastname: Kannan
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D017931:DNA Primers; D050699:E2F3 Transcription Factor / Q000235:genetics*; D005260:Female; D020868:Gene Silencing; D006801:Humans; D007150:Immunohistochemistry; D034741:RNA, Small Interfering / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D050699:E2F3 Transcription Factor; C098319:E2F3 protein, human; D034741:RNA, Small Interfering