title: [Correlation study of estrogen receptor with peripheral blood cytokines and serum markers in primary biliary cirrhosis patients].
abstract: OBJECTIVE
  To investigate the correlation between ER-a in the liver and cytokines of T lymphocytes subsets and serum signatures in PBC patients.
  
  
  METHODS
  The research is performed with cross-sectional study. 80 PBC women patients without treatment were enrolled in PBC group, 10 healthy women as baseline-matched in healthy-control group, and 20 patients with non-autoimmune liver disease in non-PBC control group. The expression of IL-6, IL-8, IL-22, TNFa, IFNgamma, AMA-M2, Sp100 and gp210 were analyzed in Peripheral Blood using ELISA in all groups, and ER-a of patients were performed on tissues from liver biopsies in PBC group and non-PBC control group with immunohistochemistry. Spearman correlation test were performed on the indices to identified the association of all Parameters. numerical data were compared with Wilcoxon rank-sum test.
  
  
  RESULTS
  Compared with healthy-control group, expression of serum cytokines are significantly higher in PBC and non-PBC groups (P less than 0.01), while no significant difference were observed between PBC and non-PBC groups. The positive rate of ER-a in PBC patients liver tissues in PBC group is higher than that in non-PBC group (Z=4.82, P less than 0.01). Expression of ER-a is positively correlated with positive rates of AMA-M2 antibody, Sp100 and gP210 of tissues of PBC patients ( r=0.898, 0.819, 0.814, P less than 0.01). ER-a is positive correlated with the expression of cytokines, among which the coefficient of correlation of IL-22, TNFa, IFNgamma is more than 0.7 (r=0.71, 0.89, 0.82, P less than 0.01), AMA-M2, Sp100, gp210 is negative in serum of non-PBC control group. No obviously correlations were indicated between the expression of ER-a and cytokines.
  
  
  CONCLUSIONS
  A high level of expression of cytokines in the serum might be one of the factors of etiopathogenesis of PBC.
authors.affiliation: Longhua Hospital Shanghai University of TCM.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Qing-mei
authors.initials: QM
authors.lastname: Li
authors.forename: Hui-hui
authors.initials: HH
authors.lastname: DU
authors.forename: Li-qiong
authors.initials: LQ
authors.lastname: Wang
authors.forename: Yi-bo
authors.initials: YB
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D047628:Estrogen Receptor alpha / Q000378:metabolism; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D016209:Interleukin-8 / Q000097:blood; D007378:Interleukins / Q000097:blood; D008099:Liver / Q000378:metabolism*; D008105:Liver Cirrhosis, Biliary / Q000097:blood* / Q000276:immunology*; D008875:Middle Aged; D000097043:Interleukin-22
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D047628:Estrogen Receptor alpha; D015850:Interleukin-6; D016209:Interleukin-8; D007378:Interleukins; D000097043:Interleukin-22; C506487:ESR1 protein, human