title: Short communication: simultaneous analysis of reducing sugars and 5-hydroxymethyl-2-furaldehyde at a low concentration by high performance anion exchange chromatography with electrochemical detector, compared with HPLC with refractive index detector.
abstract: A simultaneous analysis of reducing sugars and 5-hydroxymethyl-2-furaldehyde of the Maillard reaction products was detailed. It was based on a high performance anion exchange chromatography with electrochemical detector system and an HPLC with refractive index detector. Results showed that high performance anion exchange chromatography with electrochemical detector using a CarboPac PA-1 column (Dionex Corp., Sunnyvale, CA) was more suitable for reducing sugars and 5-hydroxymethyl-2-furaldehyde determination, especially for trace analysis. The lowest detectable limit of reducing sugars and 5-hydroxymethyl-2-furaldehyde was 0.00005 mol/L in this experiment. However, HPLC with a refractive index detector always produces a tailing peak for 5-hydroxymethyl-2-furaldehyde, and mannose and fructose cannot be absolutely separated. The results of the present study could provide a more sensitive means for 5-hydroxymethyl-2-furaldehyde and reducing sugar detection.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Y-G
authors.initials: YG
authors.lastname: Guan
authors.forename: P
authors.initials: P
authors.lastname: Yu
authors.forename: S-J
authors.initials: SJ
authors.lastname: Yu
authors.forename: X-B
authors.initials: XB
authors.lastname: Xu
authors.forename: X-L
authors.initials: XL
authors.lastname: Wu
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D005632:Fructose / Q000032:analysis; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D005947:Glucose / Q000032:analysis; D015416:Maillard Reaction; D008358:Mannose / Q000032:analysis; D012031:Refractometry
chemical_list: D002241:Carbohydrates; D005632:Fructose; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde; D005947:Glucose; D008358:Mannose