title: A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells.
abstract: We describe a rapid method for monitoring the cell growth and decline phases in suspension cultures of animal cells. During the cell growth phase, ultraviolet (UV)-absorbing components in the medium are consumed, but at later times as cells begin to die, UV-absorbing molecules such as proteins are released into the medium. Measuring the absorbance at 280nm (A(280)) with a NanoDrop spectrophotometer, an inverse correlation between the onset of the cell decline phase and A(280) was observed. This simple method can be applied to quickly determine the beginning of the decline phase of cultures of mammalian and insect cells in suspension.
authors.affiliation: Laboratory of Cellular Biotechnology, École Polytechnique Fédérale de Lausanne (EPFL), School of Life Sciences, CH-1015 Lausanne, Switzerland.
authors.forename: Patrik O
authors.initials: PO
authors.lastname: Michel
authors.forename: Christophe
authors.initials: C
authors.lastname: Degen
authors.forename: Mélanie
authors.initials: M
authors.lastname: Hubert
authors.forename: Lucia
authors.initials: L
authors.lastname: Baldi
authors.forename: David L
authors.initials: DL
authors.lastname: Hacker
authors.forename: Florian M
authors.initials: FM
authors.lastname: Wurm
mesh_terms: D000818:Animals; D016466:CHO Cells; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000502:physiology; D006224:Cricetinae; D003412:Cricetulus; D057809:HEK293 Cells; D006801:Humans; D036103:Nanotechnology; D013056:Spectrophotometry, Ultraviolet