title: Glucose-regulated protein 75 overexpression attenuates ionizing radiation-mediated             injury in PC12 cells by inducing the expression of topoisomerase IIα.
abstract: Glucose-regulated protein 75 (Grp75), also known as mortalin/mthsp70/PBP74,             is a member of the heat-shock protein 70 (HSP70) family. Grp75 is known to protect             cells from stress-induced injury. Previous studies have shown that the expression             of Grp75 is upregulated by a low dose of ionizing radiation (IR). To evaluate             the protective role of Grp75 on cell proliferation in response to IR, Grp75 was             overexpressed in a rat adrenal pheochromocytoma cell line (PC12). It was revealed             that Grp75 overexpression desensitized PC12 cells to IR-mediated cell injury.             In addition, the expression of topoisomerase IIα (Topo IIα) was downregulated             in PC12 cells following γ-ray IR. The effect of Grp75 overexpression on Topo IIα             expression was examined. It was revealed that Grp75 overexpression reversed the             inhibitory effect of IR on Topo IIα expression. In conclusion, the data indicated             that Grp75 overexpression attenuates IR-induced injury in PC12 cells by maintaining             the expression of Topo IIα.
authors.affiliation: Department of Cellular and Genetic Medicine, Shanghai Medical                     College, Fudan University, Shanghai 200032, P.R. China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Guo
authors.forename: Ling
authors.initials: L
authors.lastname: Yang
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Zuojun
authors.initials: Z
authors.lastname: Xie
authors.forename: Wen
authors.initials: W
authors.lastname: Liu
authors.forename: Ji
authors.initials: J
authors.lastname: Zuo
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism*; D004250:DNA Topoisomerases, Type II / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation / Q000528:radiation effects*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D016716:PC12 Cells; D011839:Radiation, Ionizing; D051381:Rats
chemical_list: D000951:Antigens, Neoplasm; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D008565:Membrane Proteins; C018551:glucose-regulated proteins; D004250:DNA Topoisomerases, Type II