title: Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500, a product suspected to possess doping potential.
abstract: The formulation TB-500 is suspected to be used as doping agent in sport. This work describes the detection and the identification of the N-terminal acetylated 17-23 fragment of human thymosin beta 4 (Ac-LKKTETQ) in TB-500 by means of high-performance liquid chromatography/high resolution mass spectrometry using an Orbitrap Exactive benchtop mass spectrometer. Ac-LKKTETQ was also synthesized by solid-phase peptide synthesis, and an analytical strategy for detection in plasma and urine by high-performance liquid chromatography/low resolution triple-quadrupole mass spectrometry was suggested.
authors.affiliation: Doping Control Laboratory (DoCoLab), Department of Clinical Chemistry, Microbiology and Immunology, Ghent University (UGent), Technologiepark 30, B-9052 Zwijnaarde, Belgium. Simone.Esposito@UGent.be
authors.forename: Simone
authors.initials: S
authors.lastname: Esposito
authors.forename: Koen
authors.initials: K
authors.lastname: Deventer
authors.forename: Jan
authors.initials: J
authors.lastname: Goeman
authors.forename: Johan
authors.initials: J
authors.lastname: Van der Eycken
authors.forename: Peter
authors.initials: P
authors.lastname: Van Eenoo
mesh_terms: D000107:Acetylation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D058573:Performance-Enhancing Substances / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013947:Thymosin / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D000099201:Thymosin beta(4)
chemical_list: D058573:Performance-Enhancing Substances; D013947:Thymosin; D000099201:Thymosin beta(4)