title: Label-free polypeptide-based enzyme detection using a graphene-nanoparticle hybrid sensor.
abstract: A graphene-nanoparticle (NP) hybrid biosensor that utilizes an electrical hysteresis change to detect the enzymatic activity and concentration of Carboxypeptidase B was developed. The results indicate that the novel graphene-NP hybrid biosensor, utilizing electrical hysteresis, has the ability to detect concentrations of targeted enzyme on the micromolar scale. Furthermore, to the knowledge of the authors, this is the first demonstration of a graphene-based biosensor that utilizes a hysteresis change resulting from metallic NPs assembled on a graphene surface.
authors.affiliation: Department of Chemistry and Chemical Biology, Institute for Advanced Materials Devices, and Nanotechnology (IAMDN), Rutgers The State University of New Jersey, Piscataway, NJ 08854, USA.
authors.forename: Sung
authors.initials: S
authors.lastname: Myung
authors.forename: Perry T
authors.initials: PT
authors.lastname: Yin
authors.forename: Cheoljin
authors.initials: C
authors.lastname: Kim
authors.forename: Jaesung
authors.initials: J
authors.lastname: Park
authors.forename: Aniruddh
authors.initials: A
authors.lastname: Solanki
authors.forename: Pavel Ivanoff
authors.initials: PI
authors.lastname: Reyes
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Lu
authors.forename: Kwang S
authors.initials: KS
authors.lastname: Kim
authors.forename: Ki-Bum
authors.initials: KB
authors.lastname: Lee
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D043424:Carboxypeptidase B / Q000032:analysis* / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004789:Enzyme Activation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation; D010455:Peptides / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D010455:Peptides; D006108:Graphite; D043424:Carboxypeptidase B