title: Demonstration of type II latency in T lymphocytes of Epstein-Barr Virus-associated hemophagocytic lymphohistiocytosis.
abstract: Epstein-Barr virus (EBV) is the most common infectious cause of non-genetic hemophagocytic lymphohistiocytosis (HLH). To investigate EBV-infected lymphocytes and immune dysfunction in EBV-associated HLH, blood samples from a 6-year-old boy were longitudinally analyzed using molecular techniques. EBV-positive lymphocytes were detected as CD5(+) , CD8(+) , and/or HLA DR(+) lymphocytes on Day 25 of the disease, mostly disappearing thereafter. CD8(+) cells specific for lytic antigen BRLF1 were detected, but cells specific for latent antigens EBNA3 and LMP2 were not. EBV genes EBNA1, LMP1, LMP2, EBER1, BARTs were detected, suggesting a latency type II gene expression pattern in this case.
authors.affiliation: Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan. yoshi-i@med.nagoya-u.ac.jp
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Ito
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kawamura
authors.forename: Seiko
authors.initials: S
authors.lastname: Iwata
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Kawada
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kimura
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002648:Child; D020031:Epstein-Barr Virus Infections / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051359:Lymphohistiocytosis, Hemophagocytic / Q000276:immunology* / Q000821:virology*; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction