title: [Exploration of the association of H. pylori and EBV infection with cardiac and distal gastric adenocarcinoma among residents in Cixian County, a high-risk area of esophgeal cancer in Hebei province].
abstract: OBJECTIVE
  To evaluate the H. pylori and Epstein-Barr virus infection in cardiac and distal gastric adenocarcinoma tissues in residents in Cixian county, a high risk area of esophageal cancer in Hebei province, and to explore the putative role of H. pylori and Epstein-Barr virus infection in the carcinogenesis of adenocarcinoma at different subsites of stomach.
  
  
  METHODS
  H. pylori and Epstein-Barr virus latent membrane protein 1 (EBV-LMP1) immunopositivities were determined by Elivision(TM) plus immunohistochemical staining in 190 gastric adenocarcinoma tissues including 144 cases of cardiac adenocarcinoma and 46 cases of distal gastric adenocarcinoma. The relationship between H. pylori and Epstein-Barr virus infection and the subsite, Laurén type as well as other clinicopathological features of gastric adenocarcinoma were analyzed.
  
  
  RESULTS
  No significant difference was found between the H. pylori detection rates in cardiac and distal gastric adenocarcinomas(56.9% vs. 65.2%, P > 0.05). The detection rate of H. pylori in intestinal type was significantly higher than that in the diffuse type distal gastric adenocarcinomas (71.8% vs. 28.6%, P < 0.05). No positive expression of EBV-LMP1 was found in the gastric adenocarcinomas in this study.
  
  
  CONCLUSIONS
  No significant differences in H. pylori and EBV-LMP1 infections were found between cardiac and distal gastric adenocarcinomas in Cixian county. H. pylori infection is related with the intestinal type of distal gastric adenocarcinoma.
authors.affiliation: Department of Pathology, the Second Hospital of Hebei Medical University, Shijiazhuang 050017, China.
authors.forename: Dai-zhong
authors.initials: DZ
authors.lastname: Wang
authors.forename: Xiang-hong
authors.initials: XH
authors.lastname: Zhang
authors.forename: Wen-xin
authors.initials: WX
authors.lastname: Wu
authors.forename: Yu-mei
authors.initials: YM
authors.lastname: Ma
authors.forename: Ai-rong
authors.initials: AR
authors.lastname: Cui
authors.forename: Wei-na
authors.initials: WN
authors.lastname: Liu
authors.forename: Xue-min
authors.initials: XM
authors.lastname: Li
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology / Q000473:pathology / Q000821:virology; D000368:Aged; D002299:Cardia; D002681:China; D020031:Epstein-Barr Virus Infections / Q000473:pathology*; D005260:Female; D016481:Helicobacter Infections / Q000473:pathology*; D016480:Helicobacter pylori / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000382:microbiology / Q000473:pathology / Q000821:virology; D014763:Viral Matrix Proteins / Q000378:metabolism
chemical_list: C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins