title: Idebenone treatment mediates the effect of menadione oxidative stress damage in Saccharomyces cerevisiae.
abstract: We investigated the damage caused by oxidative stress using the yeast Saccharomyces cerevisiae as a model biological system. After inducing oxidative stress with menadione, we were able to evaluate the extent of cellular oxidative stress by utilizing 2',7'-dichlorofluorescein diacetate (DCFH-DA) as a marker of the presence of reactive oxygen species. Cells were grown on different carbon sources in order to compare fermentative and oxidative metabolism. Under these conditions we evaluated the effectiveness of idebenone (2,3-dimethoxy-5-methyl-6-(10- hydroxydecyl)-1,4-benzoquinone) as a molecule that could relieve menadione-induced growth inhibition in Saccharomyces cerevisiae.
authors.affiliation: Departamento de Bioquimica Humana, Instituto de Investigaciones Biomedicas (INBIOMED) Facultad de Medicina, Universidad de Buenos Aires, Argentina.
authors.forename: Oliver
authors.initials: O
authors.lastname: Gamondi
authors.forename: Sebastián
authors.initials: S
authors.lastname: Chapela
authors.forename: Inés
authors.initials: I
authors.lastname: Nievas
authors.forename: Isabel
authors.initials: I
authors.lastname: Burgos
authors.forename: Manuel
authors.initials: M
authors.lastname: Alonso
authors.forename: Carlos
authors.initials: C
authors.lastname: Stella
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D005285:Fermentation; D005452:Fluoresceins / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000502:physiology; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000494:pharmacology; D024483:Vitamin K 3 / Q000633:toxicity
chemical_list: D000975:Antioxidants; D005452:Fluoresceins; D017382:Reactive Oxygen Species; D014451:Ubiquinone; C029569:diacetyldichlorofluorescein; D024483:Vitamin K 3; C036619:idebenone