title: Signal amplification based on DNA hybridization-dehybridization reaction on the surface of magnet submicrobeads for ultrasensitive DNA detection.
abstract: A new signal amplification strategy based on DNA hybridization-dehybridization reaction on the surface of magnetic submicrobeads (MSBs) for fluorescence detection of ultrasensitive DNA was developed. In this strategy, MSBs modified with probe DNA (DNAp-MSBs) were bound to target DNA (t-DNA) (with a ratio of 1 : 1) captured to a substrate. The DNAp-MSBs were released from the substrate via DNA dehybridization and then hybridized with Cy5-labeled detection DNA (Cy5-DNAd). After the Cy5-DNAd and DNAp-MSBs were separated by dehybridization, the Cy5-DNAd was collected. The DNAp-MSBs were then hybridized with other Cy5-DNAd to initiate the next hybridization-dehybridization round. This recycling of the hybridization-dehybridization process on the surface of the DNAp-MSBs was repeated multiple times to accumulate Cy5-DNAd. Finally, fluorescence intensity of the collected Cy5-DNAd was measured. Using this strategy, the limit of detection for determination of t-DNA was 8.5 × 10(-15) mol L(-1) for 11 cycles. The ultrasensitive assay was used to quantify ribosomal protein, large, P2 (RPLP2) mRNA in human breast cancer cells.
authors.affiliation: School of Chemistry and Chemical Engineering, Shandong University, Jinan, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Shen
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.forename: Wenrui
authors.initials: W
authors.lastname: Jin
mesh_terms: D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D004247:DNA / Q000032:analysis*; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D008280:Magnetics; D009693:Nucleic Acid Hybridization; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013499:Surface Properties
chemical_list: D002232:Carbocyanines; D004269:DNA, Bacterial; D010750:Phosphoproteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C036483:T-DNA; C085321:cyanine dye 5; C031937:phosphoprotein P2, ribosomal; D004247:DNA