title: TFAP2B influences the effect of dietary fat on weight loss under energy restriction.
abstract: BACKGROUND
  Numerous gene loci are related to single measures of body weight and shape. We investigated if 55 SNPs previously associated with BMI or waist measures, modify the effects of fat intake on weight loss and waist reduction under energy restriction.
  
  
  RESULTS
  Randomized controlled trial of 771 obese adults. (
  
  
  BACKGROUND
  ISRCTN25867281.) One SNP was selected for replication in another weight loss intervention study of 934 obese adults. The original trial was a 10-week 600 kcal/d energy-deficient diet with energy percentage from fat (fat%) in range of 20-25 or 40-45. The replication study used an 8-weeks diet of 880 kcal/d and 20 fat%; change in fat% intake was used for estimation of interaction effects. The main outcomes were intervention weight loss and waist reduction. In the trial, mean change in fat% intake was -12/+4 in the low/high-fat groups. In the replication study, it was -23/-12 among those reducing fat% more/less than the median. TFAP2B-rs987237 genotype AA was associated with 1.0 kg (95% CI, 0.4; 1.6) greater weight loss on the low-fat, and GG genotype with 2.6 kg (1.1; 4.1) greater weight loss on the high-fat (interaction p-value; p = 0.00007). The replication study showed a similar (non-significant) interaction pattern. Waist reduction results generally were similar. Study-strengths include (i) the discovery study randomised trial design combined with the replication opportunity (ii) the strict dietary intake control in both studies (iii) the large sample sizes of both studies. Limitations are (i) the low minor allele frequency of the TFAP2B polymorphism, making it hard to investigate non-additive genetic effects (ii) the different interventions preventing identical replication-discovery study designs (iii) some missing data for non-completers and dietary intake. No adverse effects/outcomes or side-effects were observed.
  
  
  CONCLUSIONS
  Under energy restriction, TFAP2B may modify the effect of dietary fat intake on weight loss and waist reduction.
authors.affiliation: Institute of Preventive Medicine, Copenhagen University Hospital, Frederiksberg, Denmark. tst@ipm.regionh.dk
authors.forename: Tanja
authors.initials: T
authors.lastname: Stocks
authors.forename: Lars
authors.initials: L
authors.lastname: Angquist
authors.forename: Karina
authors.initials: K
authors.lastname: Banasik
authors.forename: Marie N
authors.initials: MN
authors.lastname: Harder
authors.forename: Moira A
authors.initials: MA
authors.lastname: Taylor
authors.forename: Jörg
authors.initials: J
authors.lastname: Hager
authors.forename: Peter
authors.initials: P
authors.lastname: Arner
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Oppert
authors.forename: J Alfredo
authors.initials: JA
authors.lastname: Martinez
authors.forename: Jan
authors.initials: J
authors.lastname: Polak
authors.forename: Francis
authors.initials: F
authors.lastname: Rousseau
authors.forename: Dominique
authors.initials: D
authors.lastname: Langin
authors.forename: Stephan
authors.initials: S
authors.lastname: Rössner
authors.forename: Claus
authors.initials: C
authors.lastname: Holst
authors.forename: Ian A
authors.initials: IA
authors.lastname: MacDonald
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Kamatani
authors.forename: Andreas F H
authors.initials: AF
authors.lastname: Pfeiffer
authors.forename: Marie
authors.initials: M
authors.lastname: Kunesova
authors.forename: Wim H M
authors.initials: WH
authors.lastname: Saris
authors.forename: Torben
authors.initials: T
authors.lastname: Hansen
authors.forename: Oluf
authors.initials: O
authors.lastname: Pedersen
authors.forename: Arne
authors.initials: A
authors.lastname: Astrup
authors.forename: Thorkild I A
authors.initials: TI
authors.lastname: Sørensen
mesh_terms: D000328:Adult; D000886:Anthropometry; D015992:Body Mass Index; D004041:Dietary Fats; D002149:Energy Intake; D004734:Energy Metabolism; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D012107:Research Design; D050656:Transcription Factor AP-2 / Q000235:genetics / Q000502:physiology*; D055105:Waist Circumference; D015431:Weight Loss / Q000235:genetics*
chemical_list: D004041:Dietary Fats; C493677:TFAP2B protein, human; D050656:Transcription Factor AP-2