title: CD166(pos) subpopulation from differentiated human ES and iPS cells support repair of acute lung injury.
abstract: Previous efforts to derive lung progenitor cells from human embryonic stem (hES) cells using embryoid body formation or stromal feeder cocultures had been limited by low efficiencies. Here, we report a step-wise differentiation method to drive both hES and induced pluripotent stem (iPS) cells toward the lung lineage. Our data demonstrated a 30% efficiency in generating lung epithelial cells (LECs) that expresses various distal lung markers. Further enrichment of lung progenitor cells using a stem cell marker, CD166 before transplantation into bleomycin-injured NOD/SCID mice resulted in enhanced survivability of mice and improved lung pulmonary functions. Immunohistochemistry of lung sections from surviving mice further confirmed the specific engraftment of transplanted cells in the damaged lung. These cells were shown to express surfactant protein C, a specific marker for distal lung progenitor in the alveoli. Our study has therefore demonstrated the proof-of-concept of using iPS cells for the repair of acute lung injury, demonstrating the potential usefulness of using patient's own iPS cells to prevent immune rejection which arise from allogenic transplantation.
authors.affiliation: Department of Stem Cell and Developmental Biology, Genome Institute of Singapore, Genome, Singapore. bsoh@fas.harvard.edu
authors.forename: Boon Seng
authors.initials: BS
authors.lastname: Soh
authors.forename: Dahai
authors.initials: D
authors.lastname: Zheng
authors.forename: Julie Su
authors.initials: JS
authors.lastname: Li Yeo
authors.forename: Henry He
authors.initials: HH
authors.lastname: Yang
authors.forename: Shi Yan
authors.initials: SY
authors.lastname: Ng
authors.forename: Lan Hiong
authors.initials: LH
authors.lastname: Wong
authors.forename: Wencai
authors.initials: W
authors.lastname: Zhang
authors.forename: Pin
authors.initials: P
authors.lastname: Li
authors.forename: Massimo
authors.initials: M
authors.lastname: Nichane
authors.forename: Atasha
authors.initials: A
authors.lastname: Asmat
authors.forename: Poo Sing
authors.initials: PS
authors.lastname: Wong
authors.forename: Peng Cheang
authors.initials: PC
authors.lastname: Wong
authors.forename: Lin Lin
authors.initials: LL
authors.lastname: Su
authors.forename: Sakis A
authors.initials: SA
authors.lastname: Mantalaris
authors.forename: Jia
authors.initials: J
authors.lastname: Lu
authors.forename: Wa
authors.initials: W
authors.lastname: Xian
authors.forename: Frank
authors.initials: F
authors.lastname: McKeon
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Chen
authors.forename: Elaine Hsuen
authors.initials: EH
authors.lastname: Lim
authors.forename: Bing
authors.initials: B
authors.lastname: Lim
mesh_terms: D055371:Acute Lung Injury / Q000235:genetics / Q000378:metabolism* / Q000628:therapy*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology / Q000637:transplantation; D005326:Fetal Proteins / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000502:physiology / Q000637:transplantation; D051379:Mice; D020410:Activated-Leukocyte Cell Adhesion Molecule
chemical_list: D015703:Antigens, CD; D015816:Cell Adhesion Molecules, Neuronal; D005326:Fetal Proteins; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule