title: Gingival vascular functions are altered in type 2 diabetes mellitus model and/or periodontitis model.
abstract: The association of vascular reactivity between diabetes and periodontal disease has not been clarified. Gingival blood flow was measured by laser Doppler flowmetry for 31 weeks in Wistar rats, Wistar rats orally challenged with Porphyromonas gingivalis (Wistar rats + Porphyromonas gingivalis), Goto-Kakizaki rats, and Goto-Kakizaki rats orally challenged with Porphyromonas gingivalis (Goto-Kakizaki rats + Porphyromonas gingivalis). Effects of alveolar bone resorption on periodontal tissue was enhanced in Wistar rats + Porphyromonas gingivalis, and Goto-Kakizaki rats, with this effect being significantly enhanced by Goto-Kakizaki rats + Porphyromonas gingivalis. Using the L-band electron spin resonance technique, we succeeded in measuring oxidative stress as decay rate constant (K(1) and K(2)) of 3-carbamoyl-2,2,5,5-tetramethylpyrrolidin-1-yloxy in the oral and maxillofacial region of the animal models. The decay rate constant (K(1)) of 3-carbamoyl-2,2,5,5-tetramethylpyrrolidin-1-yloxy was significantly greater in the oral and maxillofacial region of Goto-Kakizaki rats + Porphyromonas gingivalis compared to Wistar rats, Wistar rats + Porphyromonas gingivalis and Goto-Kakizaki rats groups. Gingival reactive hyperemia was attenuated by periodontal disease, and this effect was also remarkable in the diabetes mellitus model. Taken together, we found that vascular endothelial function was decreased in diabetes mellitus and/or periodontal disease animal models due to increasing oxidative stress in the gingival circulation.
authors.affiliation: Department of Clinical Care Medicine, Division of Pharmacology and ESR Laboratories, Kanagawa Dental College, 82 Inaoka-cho, Yokosuka, Kanagawa 238-8580, Japan.
authors.forename: Shuta
authors.initials: S
authors.lastname: Sugiyama
authors.forename: Shun-Suke
authors.initials: SS
authors.lastname: Takahashi
authors.forename: Fumi-Aki
authors.initials: FA
authors.lastname: Tokutomi
authors.forename: Ayaka
authors.initials: A
authors.lastname: Yoshida
authors.forename: Kyo
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Yoshino
authors.forename: Satoko
authors.initials: S
authors.lastname: Wada-Takahashi
authors.forename: Toshizo
authors.initials: T
authors.lastname: Toyama
authors.forename: Kiyoko
authors.initials: K
authors.lastname: Watanabe
authors.forename: Nobushiro
authors.initials: N
authors.lastname: Hamada
authors.forename: Kazuo
authors.initials: K
authors.lastname: Todoki
authors.forename: Masaichi-Chang-Il
authors.initials: MC
authors.lastname: Lee