title: Characterization of a cobalt-specific P(1B)-ATPase.
abstract: The P(1B)-type ATPases are a ubiquitous family of P-type ATPases involved in the transport of transition metal ions. Divided into subclasses based on sequence characteristics and substrate specificity, these integral membrane transporters play key roles in metal homeostasis, metal tolerance, and the biosynthesis of metalloproteins. The P(1B-4)-ATPases have the simplest architecture of the five P(1B)-ATPase families and have been suggested to play a role in Co(2+) transport. A P(1B-4)-ATPase from Sulfitobacter sp. NAS-14.1, designated sCoaT, has been cloned, expressed, and purified. Activity assays indicate that sCoaT is specific for Co(2+). A single Co(2+) binding site is present, and optical, electron paramagnetic resonance, and X-ray absorption spectroscopic data are consistent with tetrahedral coordination by oxygen and nitrogen ligands, including a histidine and likely a water. Surprisingly, there is no evidence for coordination by sulfur. Mutation of a conserved cysteine residue, Cys 327, in the signature transmembrane Ser-Pro-Cys metal binding motif does not abolish the ATP hydrolysis activity or affect the spectroscopic analysis, establishing that this residue is not involved in the initial Co(2+) binding by sCoaT. In contrast, replacements of conserved transmembrane residues Ser 325, His 657, Glu 658, and Thr 661 with alanine abolish ATP hydrolysis activity and Co(2+) binding, indicating that these residues are necessary for Co(2+) transport. These data represent the first in vitro characterization of a P(1B-4)-ATPase and its Co(2+) binding site.
authors.affiliation: Departments of Molecular Biosciences and Chemistry, Northwestern University, Evanston, Illinois 60208, USA.
authors.forename: Eliza L
authors.initials: EL
authors.lastname: Zielazinski
authors.forename: George E
authors.initials: GE
authors.lastname: Cutsail
authors.forename: Brian M
authors.initials: BM
authors.lastname: Hoffman
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Stemmler
authors.forename: Amy C
authors.initials: AC
authors.lastname: Rosenzweig
mesh_terms: D015502:Absorptiometry, Photon; D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001665:Binding Sites; D001692:Biological Transport / Q000502:physiology; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003035:Cobalt / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D011485:Protein Binding; D041902:Rhodobacteraceae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D003035:Cobalt; D000251:Adenosine Triphosphatases