title: Investigation into the anti-thrombosis effect and contents of total saponins and flavonoids in the bioactive fraction of Naodesheng prescription.
abstract: BACKGROUND
  Naodesheng prescription is a widely used traditional Chinese medicine (TCM), and mainly applied in clinics to treat cerebral arteriosclerosis, ischemic stroke, sequelae of cerebral hemorrhage etc.
  
  
  OBJECTIVE
  The present study was designed to investigate the anti-thrombosis effects of this prescription, and to search its bioactive fraction.
  
  
  METHODS
  We evaluated the anti-thrombotic effects by measuring platelet aggregation in rats, and the clotting time and protection rate in mice. The contents of total saponins and flavonoids in the bioactive fraction were determined by UV-vis spectrophotometry.
  
  
  RESULTS
  The results showed that Naodesheng has significant anti-thrombotic and anticoagulation effects. The contents of total saponins and flavonoids in the bioactive fraction were 7.885% and 8.434%, respectively.
  
  
  CONCLUSIONS
  Such study will contribute to further exploration of Naodesheng in the therapy of thrombosis diseases and to its re-development based on the bioactive fraction and ingredients.
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, PR China. cep02cc@gmail.com
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Shu-Xian
authors.initials: SX
authors.lastname: Li
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Wang
authors.forename: Sheng-Wang
authors.initials: SW
authors.lastname: Liang
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000494:pharmacology*; D005260:Female; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D005419:Flavonoids / Q000032:analysis; D008297:Male; D051379:Mice; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000032:analysis; D013927:Thrombosis / Q000517:prevention & control
chemical_list: D000925:Anticoagulants; D004365:Drugs, Chinese Herbal; D005343:Fibrinolytic Agents; D005419:Flavonoids; D010975:Platelet Aggregation Inhibitors; D012503:Saponins; C546210:naodesheng