title: Evaluation of a silver-based electrocatalyst for the determination of hydrogen peroxide formed via enzymatic oxidation.
abstract: Silver paste electrodes modified with lyotropic phases formed from dodecyl benzenesulphonic acid and KCl were used as the reductant in the determination of the hydrogen peroxide released from the enzymatic reaction of glucose oxidase with glucose and oxygen. The response of the modified electrode to hydrogen peroxide reduction (-0.1 V vs. Ag/AgCl) was shown to suffer from interference resulting from co-localization of enzyme and substrate at the electrode surface. This interference was eradicated by the introduction of a perm-selective membrane in the form of cellulose acetate. This further facilitated immobilization of the enzyme while allowing diffusion of the generated peroxide to the electrode. The resulting configuration was shown to be capable of the analytical determination of glucose.
authors.affiliation: Department of Applied Sciences, University of the West of England, Coldharbour Lane, Bristol BS16 1QY, UK.
authors.forename: Laura
authors.initials: L
authors.lastname: Gonzalez-Macia
authors.forename: Malcolm R
authors.initials: MR
authors.lastname: Smyth
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Killard
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D002384:Catalysis; D004563:Electrochemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D012834:Silver / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D012834:Silver; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose