title: A liquid chromatography-mass spectrometry approach to study "glucosinoloma" in broccoli sprouts.
abstract: Glucosinolates are an important class of secondary plant metabolites, possessing health-promoting properties. Young broccoli plants are a very good source of glucosinolates with concentrations several times greater than in mature plants. The aim of our study was to develop a liquid chromatography-mass spectrometry and liquid chromatography/tandem mass spectrometry qualitative and quantitative method for the measure of glucosinolates in broccoli sprouts. The described method provides high sensitivity and specificity, allowing a rapid and simultaneous determination of 14 glucosinolates. The proposed method has been validated for eight glucosinolates: glucobrassicin, glucoraphanin, glucoiberin, glucoerucin, progoitrin, gluconapin, sinigrin and glucocheirolin. The linear range was 1-150 µg ml(-1), the intra-day and inter-day precision values are within 6% and 8% at the lower limit of quantification, while the overall recovery of the eight glucosinolates was 99 ± 9%. This validated method was used successfully for analysis of glucosinolates content of broccoli sprouts grown in different conditions.
authors.affiliation: National Research Institute for Food and Nutrition-INRAN, Via Ardeatina 546, 00178, Roma, Italy. maldini@inran.it
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Maldini
authors.forename: Simona
authors.initials: S
authors.lastname: Baima
authors.forename: Giorgio
authors.initials: G
authors.lastname: Morelli
authors.forename: Cristina
authors.initials: C
authors.lastname: Scaccini
authors.forename: Fausta
authors.initials: F
authors.lastname: Natella
mesh_terms: D000704:Analysis of Variance; D001937:Brassica / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005961:Glucosinolates / Q000032:analysis* / Q000378:metabolism; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005961:Glucosinolates