title: A preliminary and qualitative metallomics study of mercury in the muscle of fish from Amazonas, Brazil.
abstract: This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish species from Amazonas, Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis and subsequent evaluation of mercury by synchrotron radiation X-ray fluorescence. The fluorescence spectra revealed mercury in two protein spots. The mercury-containing protein spots showed molecular weights of 20.8 ± 0.7 and 19.8 ± 0.5 kDa and isoelectric points of 5.6 ± 0.2 and 7.5 ± 0.3, respectively.
authors.affiliation: Instituto de Biociências, UNESP, Botucatu, SP, Brazil.
authors.forename: Paula M
authors.initials: PM
authors.lastname: Moraes
authors.forename: Felipe A
authors.initials: FA
authors.lastname: Santos
authors.forename: Cilene C F
authors.initials: CC
authors.lastname: Padilha
authors.forename: José C S
authors.initials: JC
authors.lastname: Vieira
authors.forename: Luiz F
authors.initials: LF
authors.lastname: Zara
authors.forename: Pedro
authors.initials: P
authors.lastname: de M Padilha
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D001938:Brazil; D002397:Catfishes / Q000254:growth & development / Q000378:metabolism*; D060126:Characidae / Q000254:growth & development / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004781:Environmental Exposure / Q000009:adverse effects; D004784:Environmental Monitoring / Q000379:methods; D029941:Fish Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D007526:Isoelectric Point; D008628:Mercury / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*; D008667:Metalloproteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D009132:Muscles / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D045483:Rivers; D013052:Spectrometry, X-Ray Emission; D017356:Synchrotrons; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D029941:Fish Proteins; D008667:Metalloproteins; D009124:Muscle Proteins; D014874:Water Pollutants, Chemical; D008628:Mercury