title: Searching ingredients polluted by polycyclic aromatic hydrocarbons in feeds due to atmospheric or pyrolytic sources.
abstract: The primary aim of the proposed work is to propose the potential sources of pollution by polycyclic aromatic hydrocarbons (PAHs) in feeds and ingredients. To reach this propose the development of a simple, fast, quantitative and economic method for determining PAHs using liquid-liquid extraction (LLE), clean-up and detection by liquid chromatography with fluorescence detection (LC-FD) in polluting feeds and ingredients was developed. The overall method quantification limits range from 0.020 to 4.0μg/kg and analyte recoveries are between 70% and 105% with relative standard deviations (RSD) lower than 20%. Molecular patterns of PAHs were used to study their distribution in the selected samples by cluster analysis, separating them in two groups: contaminated by atmospheric or pyrolytic sources. In order to find a relationship between the nutritional composition (protein, fibre, ash and fat content), and the hypothetical toxicity of selected feeds, a partial least squared (PLS) analysis was used, showing that fibre was a major contributor. Moreover, the complete data set of 27 feed samples and 25 feed ingredients x 13 PAH concentrations were analysed by PCA to find out what ingredients were controlling PAH pollution.
authors.affiliation: Nutrition and Bromatology Group, Analytical and Food Chemistry Department, Faculty of Food Science and Technology, University of Vigo, Ourense Campus, 32004 Ourense, Spain.
authors.forename: Iria
authors.initials: I
authors.lastname: Yebra-Pimentel
authors.forename: Ricardo
authors.initials: R
authors.lastname: Fernández-González
authors.forename: Elena
authors.initials: E
authors.lastname: Martínez Carballo
authors.forename: Jesús
authors.initials: J
authors.lastname: Simal-Gándara
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D059625:Liquid-Liquid Extraction; D010944:Plants / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000302:isolation & purification*; D011506:Proteins / Q000032:analysis
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D011506:Proteins