title: Multifunctional properties of soy milk fermented by Enterococcus faecium strains isolated from raw soy milk.
abstract: Lactic acid bacteria (LAB) isolated from soy milk were used to produce a multifunctional fermented food. Seven isolates were screened for their ability to produce peptides and free isoflavones in soy milk. The antihypertensive, antioxidant, and anti-inflammatory properties of the resulting fermented soy milks were evaluated in vitro using biochemical assays. Isolates 1-5 were found to be producers of fermented soy milk with angiotensin I converting enzyme inhibitory activity (ACEI). Isolate 3 was found to be a producer of free isoflavones that increased the antioxidant and anti-inflammatory potential of fermented soy milk. LAB isolates 2-5 were submitted to genetic profiling and a characterization scheme. These isolates were identified as  Enterococcus faecium , and none of them contained virulence determinants or resistance to antibiotics. In conclusion, this study shows that the application of E. faecium isolate 3 for multifunctional food production from soy milk could be a promising strategy in the prevention therapy against cardiovascular disease.
authors.affiliation: Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martinez-Villaluenga
authors.forename: Maria Ines
authors.initials: MI
authors.lastname: Torino
authors.forename: V
authors.initials: V
authors.lastname: Martín
authors.forename: Rebeca
authors.initials: R
authors.lastname: Arroyo
authors.forename: Patricia
authors.initials: P
authors.lastname: Garcia-Mora
authors.forename: Isabel
authors.initials: I
authors.lastname: Estrella Pedrola
authors.forename: Concepcion
authors.initials: C
authors.lastname: Vidal-Valverde
authors.forename: Juan Miguel
authors.initials: JM
authors.lastname: Rodriguez
authors.forename: Juana
authors.initials: J
authors.lastname: Frias
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors; D000893:Anti-Inflammatory Agents; D000959:Antihypertensive Agents; D000975:Antioxidants; D002318:Cardiovascular Diseases / Q000517:prevention & control; D016984:Enterococcus faecium / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D055951:Functional Food / Q000032:analysis / Q000382:microbiology*; D007529:Isoflavones / Q000096:biosynthesis; D041942:Soy Milk / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000893:Anti-Inflammatory Agents; D000959:Antihypertensive Agents; D000975:Antioxidants; D007529:Isoflavones