title: EDC4 interacts with and regulates the dephospho-CoA kinase activity of CoA synthase.
abstract: Coenzyme A synthase (CoAsy) is a bifunctional enzyme which facilitates the last two steps of Coenzyme A biogenesis in higher eukaryotes. Here we describe that CoAsy forms a complex with enhancer of mRNA-decapping protein 4 (EDC4), a central scaffold component of processing bodies. CoAsy/EDC4 complex formation is regulated by growth factors and is affected by cellular stresses. EDC4 strongly inhibits the dephospho-CoA kinase activity of CoAsy in vitro. Transient overexpression of EDC4 decreases cell proliferation, and further co-expression of CoAsy diminishes this effect. Here we report that EDC4 might contribute to regulation of CoA biosynthesis in addition to its scaffold function in processing bodies.
authors.affiliation: State Key Laboratory of Molecular and Cellular Biology, Institute of Molecular Biology and Genetics, Kyiv 03680, Ukraine. d.o.gudkova@gmail.com
authors.forename: Daria
authors.initials: D
authors.lastname: Gudkova
authors.forename: Ganna
authors.initials: G
authors.lastname: Panasyuk
authors.forename: Ivan
authors.initials: I
authors.lastname: Nemazanyy
authors.forename: Alexander
authors.initials: A
authors.lastname: Zhyvoloup
authors.forename: Pascale
authors.initials: P
authors.lastname: Monteil
authors.forename: Valeriy
authors.initials: V
authors.lastname: Filonenko
authors.forename: Ivan
authors.initials: I
authors.lastname: Gout
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003065:Coenzyme A / Q000096:biosynthesis; D003600:Cytosol / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009997:Osmotic Pressure; D018384:Oxidative Stress; D011485:Protein Binding; D021381:Protein Transport; D011506:Proteins / Q000378:metabolism*; D014166:Transferases / Q000378:metabolism*
chemical_list: C507914:EDC4 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; D014166:Transferases; C509687:COASY protein, human; D003065:Coenzyme A