title: Knockdown of mediator complex subunit 19 inhibits the growth of ovarian             cancer.
abstract: Ovarian cancer causes more deaths than any other type of female reproductive             cancer. The development of new therapeutic approaches is required due to the low             survival rate using routine methods. The goal of this study was to investigate             the effect of the gene silencing of mediator complex subunit 19 (MED19) on cell             viability and tumor growth in ovarian cancer. Immunohistochemistry was used to             characterize the expression of MED19 in human ovarian cancer tissues. Lentivirus-mediated             RNAi was employed to downregulate endogenous MED19 expression in SKOV-3 and HEY             ovarian cancer cells. MTT assay, BrdU incorporation assay, colony formation assay,             cell cycle analysis and tumor xenografts in nude mice were performed to determine             the effects of MED19 silencing on cell viability and tumor growth in vitro and             in vivo. The data showed that the expression of MED19 in human ovarian cancer             tissues correlated with the level of tumor malignancy. The downregulation of MED19             in ovarian cancer cells significantly inhibited cell proliferation and colony             formation in vitro and led to cell cycle arrest in the G0/G1 phase. MED19 RNAi             significantly inhibited ovarian cancer tumor growth in engrafted nude mice. Our             findings reveal that the knockdown of MED19 by lentivirus-mediated RNAi may be             useful in the treatment of human ovarian cancer.
authors.affiliation: Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, PR China.
authors.forename: Yingtao
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiang
authors.initials: X
authors.lastname: Tao
authors.forename: Lingling
authors.initials: L
authors.lastname: Fan
authors.forename: Luoqi
authors.initials: L
authors.lastname: Jia
authors.forename: Chao
authors.initials: C
authors.lastname: Gu
authors.forename: Youji
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D056892:Mediator Complex / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: C558319:MED19 protein, human; D056892:Mediator Complex; D034741:RNA, Small Interfering