title: The α-ketoglutarate/Fe(II)-dependent dioxygenase VldW is responsible for the formation of validamycin B.
abstract: From A to B: Through detailed biochemical investigations, we discovered that VldW, an α-ketoglutarate/Fe(II)-dependent dioxygenase, regioselectively hydroxylates validamycin A to validamycin B. The results provide insights into the biosynthesis of hydroxylated validamycins and could be used to control the metabolic outcomes of the validamycin pathway.
authors.affiliation: Department of Pharmaceutical Sciences, Oregon State University, 203 Pharmacy Building, Corvallis, OR 97331, USA.
authors.forename: Khaled H
authors.initials: KH
authors.lastname: Almabruk
authors.forename: Shumpei
authors.initials: S
authors.lastname: Asamizu
authors.forename: Ada
authors.initials: A
authors.lastname: Chang
authors.forename: Sheril G
authors.initials: SG
authors.lastname: Varghese
authors.forename: Taifo
authors.initials: T
authors.lastname: Mahmud
mesh_terms: D049308:Dioxygenases / Q000378:metabolism*; D016695:Glycosyltransferases / Q000378:metabolism; D007294:Inositol / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism*; D013237:Stereoisomerism; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology*
chemical_list: D007656:Ketoglutaric Acids; D007294:Inositol; C003749:validamycins; D049308:Dioxygenases; D016695:Glycosyltransferases; C531432:ValG protein, Streptomyces hygroscopicus