title: The effect of pulsed magnetic field on the molecular uptake and medium conductivity of leukemia cell.
abstract: The cell membrane acts as a barrier that hinders free entrance of most hydrophilic molecules into the cell. Owing to the numerous applications, the introduction of non-permeate molecules into biologic cells has drawn considerable attention in the past years. The aim of our study was to investigate the effect of time-varying magnetic field on transmembrane molecular transport by measuring bleomycin cytotoxicity and conductivity modifying in K562 cells. The cells were exposed to magnetic pulses of 2.2 T strength peak and about 250-μs duration via Magstim stimulator and double 70-mm coil. Three different frequencies of 0.25, 1, and 10 Hz pulses for 56,112, and 28 numbers of pulses, respectively, were applied (nine experimental groups) and uptake and conductivity was measured in each group. Our results show that time-varying magnetic field increase transmembrane molecular transport and media conductivity; this enhancement is greater for 28 pulses with 1 Hz frequency. The observed uptake enhancement due to magnetic exposure is considerable.
authors.affiliation: Department of Medical Physics, Tarbiat Modares University, Tehran, Iran.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Shankayi
authors.forename: S M P
authors.initials: SM
authors.lastname: Firoozabadi
authors.forename: Mahsa G
authors.initials: MG
authors.lastname: Mansurian
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001761:Bleomycin / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology*; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D004553:Electric Conductivity; D004574:Electromagnetic Fields; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors
chemical_list: D000903:Antibiotics, Antineoplastic; D001761:Bleomycin