title: Synthesis and characterization of doxorubicin modified ZnO/PEG nanomaterials and its photodynamic action.
abstract: The aim of this study is to investigate a new strategy of combined application of ZnO/PEG nanospheres with anticancer drug of doxorubicin (DOX) in photodynamic therapy (PDT). We were able to fabricate ZnO/PEG nanospheres as the drug carrier of DOX in drug delivery system. The combination of DOX-ZnO/PEG nanocomposites induced the remarkable improvement in the anti-tumor activity, which has been demonstrated by antibacterial activity, drug release and DNA cleavage study. Furthermore, the possible mechanism was explored by optical spectroscopic studies and EPR - spin trapping technique. It was noted that the photodynamic activity of the non-cytotoxic DOX loaded ZnO/PEG nanocomposite could considerably increase cancer cell injury mediated by reactive oxygen species (ROS) under UV irradiation. In our observations demonstrated that ZnO/PEG nanosphere could obviously increase the intracellular concentration of DOX and enhance its potential anti-tumor efficiency, indicating that ZnO/PEG nanosphere could act as an efficient drug delivery carrier importing DOX into target cancer cells. Nearly 91% of loaded drug was released within 26 h of incubation of conjugates in vitro in an acidic environment. It suggests that there is an efficient drug release of DOX from DOX-ZnO/PEG nanocomposite. DOX loaded on ZnO/PEG nanomaterials showed antibacterial activity was more pronounced with Gram-positive than Gram-negative bacteria under visible light. DOX-ZnO/PEG nanocomposites were effective against HeLa cell lines under in vitro condition and photocleavage of DNA. This result indicated that ZnO/PEG nanomaterials can be used as a nanocarrier for drug delivery system for PDT.
authors.affiliation: P.G. Department of Chemistry, Cardamom Planters' Association College, Bodinayakanur 625 513, Tamil Nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Hariharan
authors.forename: S
authors.initials: S
authors.lastname: Senthilkumar
authors.forename: A
authors.initials: A
authors.lastname: Suganthi
authors.forename: M
authors.initials: M
authors.lastname: Rajarajan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D002214:Capsules; D002470:Cell Survival / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D053837:DNA Cleavage / Q000187:drug effects / Q000528:radiation effects; D004317:Doxorubicin / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D055620:Optical Phenomena; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000737:chemistry*; D026082:Singlet Oxygen / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D002214:Capsules; D004337:Drug Carriers; D017319:Photosensitizing Agents; D026082:Singlet Oxygen; D011092:Polyethylene Glycols; D004317:Doxorubicin; D015034:Zinc Oxide