title: Characterization of the secretion pathway of the collagen adhesin EmaA of Aggregatibacter actinomycetemcomitans.
abstract: The extracellular matrix protein adhesin A (EmaA) surface antennae-like structures of the periodontal pathogen Aggregatibacter actinomycetemcomitans are composed of three identical protein monomers. Recently, we have demonstrated that the protein is synthesized with an extended signal peptide of 56 amino acids necessary for membrane targeting and protein translocation. In this study, EmaA secretion was demonstrated to be reliant on a chaperone-dependent secretion pathway. Deletion of secB partially reduced but did not abolish the amount of EmaA in the membrane. This observation was attributed to an increase in the synthesis of DnaK in the ΔsecB strain. Overexpression of a DnaK substitution mutant (A174T), with diminished activity, in the ΔsecB strain further reduced the amount of EmaA in the membrane. Expression of dnaK A174T in the wild-type strain did not affect the amount of EmaA in the membrane when grown under optimal growth conditions at 37°C. However, EmaA was found to be reduced when this strain was grown at heat-shock temperature. A chromosomal deletion of amino acids 16-39 of the EmaA extended signal peptide, transformed with either the wild-type or dnaK A174T-expressing plasmid, did not affect the amount of EmaA in the membrane. In addition, the level of EmaA in a ΔsecB/emaA(-) double mutant strain expressing EmaAΔ16-39 was unchanged when grown at both temperatures. The data suggest that chaperones are required for the targeting of EmaA to the membrane and a specific region of the signal peptide is necessary for secretion under stress conditions.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA.
authors.forename: X
authors.initials: X
authors.lastname: Jiang
authors.forename: T
authors.initials: T
authors.lastname: Ruiz
authors.forename: K P
authors.initials: KP
authors.lastname: Mintz
mesh_terms: D000225:Adenine; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000502:physiology; D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D016976:Aggregatibacter actinomycetemcomitans / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D002872:Chromosome Deletion; D003094:Collagen / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics; D006801:Humans; D018832:Molecular Chaperones / Q000378:metabolism; D016254:Mutagenesis, Insertional / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics; D018271:Signal Recognition Particle / Q000235:genetics; D013941:Thymine; D014169:Transformation, Bacterial / Q000235:genetics
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D018832:Molecular Chaperones; D021382:Protein Sorting Signals; C057842:SecB protein, Bacteria; D018271:Signal Recognition Particle; D003094:Collagen; D000251:Adenosine Triphosphatases; D000225:Adenine; D013941:Thymine