title: A method for rapid and simultaneous mapping of genetic loci and introgression sizes in nematode species.
abstract: Caenorhabditis briggsae is emerging as an attractive model organism not only in studying comparative biology against C. elegans, but also in developing novel experimentation avenues. In particular, recent identification of a new Caenorhabditis species, C. sp.9 with which it can mate and produce viable progeny provides an opportunity for studying the genetics of hybrid incompatibilities (HI) between the two. Mapping of a specific HI locus demands repeated backcrossing to get hold of the specific genomic region underlying an observed phenotype. To facilitate mapping of HI loci between C. briggsae and C. sp.9, an efficient mapping method and a genetic map ideally consisting of dominant markers are required for systematic introgression of genomic fragments between the two species. We developed a fast and cost-effective method for high throughput mapping of dominant loci with resolution up to 1 million bps in C. briggsae. The method takes advantage of the introgression between C. briggsae and C. sp.9 followed by PCR genotyping using C. briggsae specific primers. Importantly, the mapping results can not only serve as an effective way for estimating the chromosomal position of a genetic locus in C. briggsae, but also provides size information for the introgression fragment in an otherwise C. sp.9 background. In addition, it also helps generate introgression line as a side-product that is invaluable for the subsequent mapping of HI loci. The method will greatly facilitate the construction of a genetic map consisting of dominant markers and pave the way for systematic isolation of HI loci between C. briggsae and C. sp.9 which has so far not been attempted between nematode species. The method is designed for mapping of a dominant allele, but can be easily adapted for mapping of any other type of alleles in any other species if introgression between a sister species pair is feasible.
authors.affiliation: Department of Biology, Faculty of Science, Hong Kong Baptist University, Hong Kong, China.
authors.forename: Cheung
authors.initials: C
authors.lastname: Yan
authors.forename: Yu
authors.initials: Y
authors.lastname: Bi
authors.forename: Da
authors.initials: D
authors.lastname: Yin
authors.forename: Zhongying
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002107:Caenorhabditis / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D005260:Female; D056426:Genetic Loci / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D023281:Genomics; D060005:Genotyping Techniques; D006824:Hybridization, Genetic; D008297:Male; D013997:Time Factors; D019076:Transgenes / Q000235:genetics
chemical_list: D017931:DNA Primers; D005819:Genetic Markers