title: Endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency.
abstract: The therapeutic potential of stem cells is limited by the non-uniformity of their phenotypic state. Thus it would be advantageous to noninvasively monitor stem cell status. Driven by this challenge, we employed multidimensional multiphoton microscopy to quantify changes in endogenous fluorescence occurring with pluripotent stem cell differentiation. We found that global and cellular-scale fluorescence lifetime of human embryonic stem cells (hESC) and murine embryonic stem cells (mESC) consistently decreased with differentiation. Less consistent were trends in endogenous fluorescence intensity with differentiation, suggesting intensity is more readily impacted by nuances of species and scale of analysis. What emerges is a practical and accessible approach to evaluate, and ultimately enrich, living stem cell populations based on changes in metabolism that could be exploited for both research and clinical applications.
authors.affiliation: Laboratory for Optical and Computational Instrumentation, University of Wisconsin, Madison, Wisconsin, United States of America.
authors.forename: Jayne M
authors.initials: JM
authors.lastname: Squirrell
authors.forename: Jimmy J
authors.initials: JJ
authors.lastname: Fong
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Ariza
authors.forename: Amber
authors.initials: A
authors.lastname: Mael
authors.forename: Kassondra
authors.initials: K
authors.lastname: Meyer
authors.forename: Nirupama K
authors.initials: NK
authors.lastname: Shevde
authors.forename: Avtar
authors.initials: A
authors.lastname: Roopra
authors.forename: Gary E
authors.initials: GE
authors.lastname: Lyons
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Kamp
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Eliceiri
authors.forename: Brenda M
authors.initials: BM
authors.lastname: Ogle
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D058732:Embryoid Bodies / Q000166:cytology / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008853:Microscopy; D057054:Molecular Imaging; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D014212:Tretinoin; D005947:Glucose