title: Replication timing in a single human chromosome 11 transferred into the Chinese hamster ovary (CHO) cell line.
abstract: DNA replication in eukaryotes initiates from discrete genomic regions, termed origins, according to a strict and often tissue-specific temporal program. However, the genetic program that controls activation of replication origins has still not been fully elucidated in mammalian cells. Previously, we measured replication timing at the sequence level along human chromosomes 11q and 21q. In the present study, we sought to obtain a greater understanding of the relationship between replication timing programs and human chromosomes by analysis of the timing of replication of a single human chromosome 11 that had been transferred into the Chinese hamster ovary (CHO) cell line by chromosome engineering. Timing of replication was compared for three 11q chromosomal regions in the transformed CHO cell line (CHO(h11)) and the original human fibroblast cell line, namely, the R/G-band boundary at 11q13.5/q14.1, the centromere and the distal telomere. We found that the pattern of replication timing in and around the R/G band boundary at 11q13.5/q14.1 was similar in CHO(h11) cells and fibroblasts. The 11q centromeric region, which replicates late in human fibroblasts, replicated in the second half of S phase in CHO(h11) cells. By contrast, however, the telomeric region at 11q25, which is late replicating in fibroblasts (and in several other human cell lines), replicated in the first half of S phase or in very early S phase in CHO(h11) cells. Our observations suggest that the replication timing programs of the R/G-band boundary and the centromeric region of human chromosome 11q are maintained in CHO(h11) cells, whereas that for the telomeric region is altered. The replication timing program of telomeric regions on human chromosomes might be regulated by specific mechanisms that differ from those for other chromosomal regions.
authors.affiliation: Department of Laboratory Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kazuki
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Oshimura
authors.forename: Toshimichi
authors.initials: T
authors.lastname: Ikemura
authors.forename: Masato
authors.initials: M
authors.lastname: Maekawa
mesh_terms: D000818:Animals; D016466:CHO Cells; D002453:Cell Cycle; D002503:Centromere / Q000235:genetics; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D006224:Cricetinae; D003412:Cricetulus; D004261:DNA Replication; D042522:DNA Replication Timing; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction; D018741:Replication Origin