title: Discrimination of gastric cancer from normal by serum RNA based on surface-enhanced Raman spectroscopy (SERS) and multivariate analysis.
abstract: OBJECTIVE
  Here, the authors explore the feasibility of discriminating cancer patients from healthy controls by serum RNA detection based on surface-enhanced Raman spectroscopy (SERS) and multivariate analysis.
  
  
  METHODS
  MgSO(4)-aggregated silver nanoparticles (Ag NP) as the SERS-active substrate presented strong SERS signals to RNA. SERS measurements were performed on two groups of serum RNA samples: one group from patients (n = 31) with gastric cancer and the other group from healthy volunteers (n = 34).
  
  
  RESULTS
  Tentative assignments of the Raman bands in the normalized SERS spectra demonstrated that there are differential expressions of circulating RNA between the gastric cancer group and the control group. Principal component analysis (PCA) combined with linear discriminate analysis (LDA) was introduced to differentiate gastric cancer from normal and achieved sensitivity of 100% and specificity of 94.1%.
  
  
  CONCLUSIONS
  This exploratory study demonstrated potential for developing serum RNA SERS analysis into a useful clinical tool for noninvasive screening and detection of cancer.
authors.affiliation: Teaching Hospital of Fujian Medical University, Fuzhou, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Xiongwei
authors.initials: X
authors.lastname: Zheng
authors.forename: Cheng
authors.initials: C
authors.lastname: He
authors.forename: Shangyuan
authors.initials: S
authors.lastname: Feng
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Lin
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.forename: Haisan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D006801:Humans; D008875:Middle Aged; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D012313:RNA / Q000097:blood*; D013059:Spectrum Analysis, Raman; D013274:Stomach Neoplasms / Q000097:blood* / Q000175:diagnosis*
chemical_list: D012313:RNA