title: First molecular characterization of Giardia duodenalis from goats in Malaysia.
abstract: In the present study, 310 faecal samples from goats from eight different farms in Malaysia were tested for the presence of Giardia using a PCR-coupled approach. The nested PCR for SSU amplified products of the expected size (∼200 bp) from 21 of 310 (6.8%) samples. Sixteen of these 21 products could be sequenced successfully and represented six distinct sequence types. Phylogenetic analysis of the SSU sequence data using Bayesian Inference (BI) identified Giardia assemblages A, B and E. The identification of the 'zoonotic' assemblages A and B suggests that Giardia-infected goats represent a possible reservoir for human giardiasis in Malaysia.
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. limailian@um.edu.my
authors.forename: Yvonne A L
authors.initials: YA
authors.lastname: Lim
authors.forename: Mohammed A K
authors.initials: MA
authors.lastname: Mahdy
authors.forename: Tiong K
authors.initials: TK
authors.lastname: Tan
authors.forename: Xiang T
authors.initials: XT
authors.lastname: Goh
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Jex
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Nolan
authors.forename: Reuben S K
authors.initials: RS
authors.lastname: Sharma
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D000818:Animals; D016054:DNA, Protozoan / Q000235:genetics*; D005243:Feces / Q000469:parasitology*; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000469:parasitology / Q000662:veterinary*; D015511:Goat Diseases / Q000469:parasitology*; D006041:Goats / Q000469:parasitology; D008296:Malaysia; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan