title: Thermally responsive phospholipid preparations for fluid steering and separation in microfluidics.
abstract: Aqueous phospholipid preparations comprised of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) are prevalent materials for biological characterization and become gel-like near physiological temperature, but have a low viscosity below 24°C. The rheology of 20% phospholipid preparations of [DMPC]/[DHPC] = 2.5 reveals that, under conditions utilized for fluid steering, the materials are shear-thinning power-law fluids with a power-law index ranging from 0.30 through 0.90. Phospholipid preparations are utilized to steer fluids in microfluidic chips and support hydrodynamic delivery of sample across a double T injection region in a chip. The fact that the phospholipids are fully integrated as a valving material as well as a separation medium is demonstrated through the separation of linear oligosaccharides labeled with 1-aminopyrene-3,6,8-trisulfonic acid.
authors.affiliation: C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV 26506, USA.
authors.forename: Xingwei
authors.initials: X
authors.lastname: Wu
authors.forename: Ted J
authors.initials: TJ
authors.lastname: Langan
authors.forename: Brandon C
authors.initials: BC
authors.lastname: Durney
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Holland
mesh_terms: D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D057446:Hydrodynamics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D010742:Phospholipid Ethers / Q000737:chemistry*; D013696:Temperature; D014783:Viscosity
chemical_list: C052298:1,2-dihexadecyl-sn-glycero-3-phosphocholine; D010742:Phospholipid Ethers; D004134:Dimyristoylphosphatidylcholine