title: Alternative diets for Chrysomya putoria, an Old World screwworm fly.
abstract: Abstract   The purpose of this study was to evaluate the post-embryonic development of Chrysomya putoria (Wiedemann 1818) (Diptera: Calliphoridae) reared on a diet of gizzard or gizzard/agar homogenate, with a diet of beef used as the control. Four replicates per treatment were performed (60 mL of each diet). The gizzard (60%), distilled water, and agar homogenate were combined in a blender. Each replicate consisted of 40 newly hatched larvae of C. putoria (5(th) generation). Each glass beaker containing a diet was inserted into a larger flask containing sawdust, which was covered with a nylon cloth held in place by an elastic band. The larvae were weighed and stored in test tubes sealed with a nylon cloth and an elastic band. The average temperature, measured with a thermohygrograph, was 20.6 °C, and the average relative humidity was 67.7%. The variation in the mean weight of mature larvae and in the duration of the larval, pupal, and total stages (newly hatched larvae to imagoes) were analyzed by Student's t-test (α = 5%), while viability was compared by ANOVA. The sex ratio was evaluated by the chi-squared test. The average duration of the period from the larval to imago stage was 8.868 days on the beef diet, 8.676 on the gizzard diet, and 9.067 on the gizzard/agar homogenate diet. Larval survival rates on these diets were 98, 92, and 73%, respectively, while pupal viabilities were 98, 91, and 71%, respectively, and larva-to-imago viabilities were 93, 83, and 64%, respectively. The duration of the pupal period differed significantly between the blowflies reared on the beef and gizzard/agar homogenate diets. The two diets proved to be good alternatives for rearing C. putoria.
authors.affiliation: Universidade Federal Rural do Rio de Janeiro - UFRRJ, Rod. BR 465, Km 7, CEP 23890-000 Seropédica, RJ, Brazil. adrianapedroso7@yahoo.com.br
authors.forename: Adriana C P
authors.initials: AC
authors.lastname: Ferraz
authors.forename: Daniele L
authors.initials: DL
authors.lastname: Dallavecchia
authors.forename: Débora Cardoso
authors.initials: DC
authors.lastname: da Silva
authors.forename: Rafaela Pereira
authors.initials: RP
authors.lastname: de Carvalho
authors.forename: Renato Geraldo
authors.initials: RG
authors.lastname: da Silva Filho
authors.forename: Valéria M
authors.initials: VM
authors.lastname: Aguiar-Coelho
mesh_terms: D000821:Animal Feed; D000818:Animals; D002417:Cattle; D002645:Chickens; D004175:Diptera / Q000254:growth & development*; D005260:Female; D007814:Larva / Q000254:growth & development; D008297:Male; D011679:Pupa / Q000254:growth & development