title: Nm23-H1 nuclear expression is associated with a more favourable prognosis in laryngeal carcinoma: univariate and multivariate analysis.
abstract: OBJECTIVE
  To use image analysis and multivariate analysis to investigate the prognostic significance of Nm23-H1 subcellular localization in a large cohort of laryngeal squamous cell carcinomas (LSCCs).
  
  
  RESULTS
  Nm23-H1 total and nuclear levels were immunohistochemically determined and calculated with an image analysis system in 104 consecutively operated LSCCs. The mean follow-up was 58.3 ± 35.1 months (median 45 months). Total Nm23-H1 levels correlated only with patient stratification by pT (P=0.01). Mean nuclear Nm23-H1 levels were lower in patients with recurrent disease (P=0.01), and disease-free survival (DFS) was longer in patients whose nuclear levels of Nm23-H1 were >2.0% than in those with levels ≤ 2.0% (P=0.019). On multivariate analysis, Nm23-H1 nuclear expression [hazard ratio (HR) 2.59, P=0.005] and N stage (HR 3.60, P=0.0001) were prognostically significant in relation to DFS.
  
  
  CONCLUSIONS
  In LSCC, Nm23-H1 nuclear expression may be useful for identifying patients at higher risk of recurrence after treatment and who might be considered for more aggressive therapy. Further investigations are needed before Nm23-H1 can be considered for use in targeted treatments for LSCC.
authors.affiliation: Department of Neurosciences, Otolaryngology Section, University of Padova, Padova, Italy. gino.marioni@unipd.it
authors.forename: Gino
authors.initials: G
authors.lastname: Marioni
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Ottaviano
authors.forename: Marco
authors.initials: M
authors.lastname: Lionello
authors.forename: Lucia
authors.initials: L
authors.lastname: Lora
authors.forename: Andrea
authors.initials: A
authors.lastname: Lovato
authors.forename: Claudia
authors.initials: C
authors.lastname: Staffieri
authors.forename: Niccolò
authors.initials: N
authors.lastname: Favaretto
authors.forename: Luciano
authors.initials: L
authors.lastname: Giacomelli
authors.forename: Edoardo
authors.initials: E
authors.lastname: Stellini
authors.forename: Alberto
authors.initials: A
authors.lastname: Staffieri
authors.forename: Stella
authors.initials: S
authors.lastname: Blandamura
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism* / Q000401:mortality; D015331:Cohort Studies; D018572:Disease-Free Survival; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007822:Laryngeal Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D054778:NM23 Nucleoside Diphosphate Kinases / Q000378:metabolism*; D011379:Prognosis; D012008:Recurrence; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases; C517938:NME1 protein, human