title: Human papillomavirus type 58 L1 virus-like particles purified by two-step chromatography elicit high levels of long-lasting neutralizing antibodies.
abstract: Human papillomavirus (HPV) type 58 is a high-risk type of HPV frequently detected in cervical cancers, especially in Eastern Asia. There are still no commercially available vaccines against HPV 58 infection. High levels of long-lasting neutralizing antibodies are crucial for long-term protection against HPV infection. Here, we have developed a two-step chromatography strategy and have purified highly pure HPV L1 proteins, which form more homogenous and uniform VLPs than those purified by CsCl ultracentrifugation. Low-dosage immunization with HPV 58 L1 VLPs alone or co-administrated with HPV 16 and HPV 18 L1 VLPs is sufficient to induce high levels of long-lasting neutralizing antibodies in mice. Our results suggest that the highly immunogenic HPV 58 L1 VLPs are a good candidate for use in developing effective vaccines against HPV 58 infection.
authors.affiliation: Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
authors.forename: Xixiu
authors.initials: X
authors.lastname: Xie
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Yanying
authors.initials: Y
authors.lastname: Xu
authors.forename: Qifeng
authors.initials: Q
authors.lastname: Bao
authors.forename: Xue
authors.initials: X
authors.lastname: Chen
authors.forename: Hongyang
authors.initials: H
authors.lastname: Liu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Xu
mesh_terms: D052685:Alphapapillomavirus / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology* / Q000302:isolation & purification; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000276:immunology* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000276:immunology / Q000821:virology*; D053918:Papillomavirus Vaccines / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D036022:Capsid Proteins; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral; D053918:Papillomavirus Vaccines