title: 17β-Estradiol enhances response of mice spleen B cells elicited by TLR9 agonist.
abstract: Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of autoantibodies against nucleic acid-associated antigens. B cells play cardinal roles in SLE. Many evidences have proved estrogen contribute to the gender bias in SLE and 17β-estradiol (E2) could accelerate the disease by regulating B cells. On the other hand, B cells express TLR9 which recognized dsDNA and played a critical role in SLE. However, the crosstalk between estrogen and TLR9 in B cells remains unknown. So we investigated the E2 effect in the presence of the TLR9 ligand CpG on mice spleen B cells. We found that the up-regulation of cell viability, life-span, co-stimulation molecules (CD40, CD86) expression, IgM secretion, TLR9 and MCM6 expression were more significant than CpG ODN or E2 stimulated alone. It may provide a new way to investigate the mechanism of how E2 modulate the B cells function in lupus.
authors.affiliation: Immunology and Reproductive Biology Lab, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Xu
authors.forename: Hongye
authors.initials: H
authors.lastname: Fan
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Li
authors.forename: Lingyun
authors.initials: L
authors.lastname: Sun
authors.forename: Yayi
authors.initials: Y
authors.lastname: Hou
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D051940:B7-2 Antigen / Q000235:genetics / Q000276:immunology; D019013:CD40 Antigens / Q000235:genetics / Q000276:immunology; D018797:Cell Cycle Proteins / Q000235:genetics / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects; D004357:Drug Synergism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007075:Immunoglobulin M / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D064151:Minichromosome Maintenance Complex Component 6; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D012737:Sex Factors; D015398:Signal Transduction; D013154:Spleen / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D051217:Toll-Like Receptor 9 / Q000819:agonists / Q000235:genetics / Q000276:immunology*; D015854:Up-Regulation
chemical_list: D051940:B7-2 Antigen; D019013:CD40 Antigens; C408982:CPG-oligonucleotide; D018797:Cell Cycle Proteins; D007075:Immunoglobulin M; D009838:Oligodeoxyribonucleotides; C495370:Tlr9 protein, mouse; D051217:Toll-Like Receptor 9; D004958:Estradiol; C491205:Mcm6 protein, mouse; D064151:Minichromosome Maintenance Complex Component 6