title: Antibacterial activity of silver bionanocomposites synthesized by chemical reduction route.
abstract: BACKGROUND
  The aim of this study is to investigate the functions of polymers and size of nanoparticles on the antibacterial activity of silver bionanocomposites (Ag BNCs). In this research, silver nanoparticles (Ag NPs) were incorporated into biodegradable polymers that are chitosan, gelatin and both polymers via chemical reduction method in solvent in order to produce Ag BNCs. Silver nitrate and sodium borohydride were employed as a metal precursor and reducing agent respectively. On the other hand, chitosan and gelatin were added as a polymeric matrix and stabilizer. The antibacterial activity of different sizes of silver nanoparticles was investigated against Gram-positive and Gram-negative bacteria by the disk diffusion method using Mueller-Hinton Agar.
  
  
  RESULTS
  The properties of Ag BNCs were studied as a function of the polymer weight ratio in relation to the use of chitosan and gelatin. The morphology of the Ag BNCs films and the distribution of the Ag NPs were also characterized. The diameters of the Ag NPs were measured and their size is less than 20 nm. The antibacterial trait of silver/chitosan/gelatin bionanocomposites was investigated. The silver ions released from the Ag BNCs and their antibacterial activities were scrutinized. The antibacterial activities of the Ag BNC films were examined against Gram-negative bacteria (E. coli and P. aeruginosa) and Gram-positive (S. aureus and M. luteus) by diffusion method using Muller-Hinton agar.
  
  
  CONCLUSIONS
  The antibacterial activity of Ag NPs with size less than 20 nm was demonstrated and showed positive results against Gram-negative and Gram-positive bacteria. The Ag NPs stabilized well in the polymers matrix.
authors.affiliation: Department of Chemistry, Faculty of Science, University Putra Malaysia, Selangor, Malaysia. mansorahmad@science.upm.edu.my.
authors.forename: Mansor
authors.initials: M
authors.lastname: Bin Ahmad
authors.forename: Jenn Jye
authors.initials: JJ
authors.lastname: Lim
authors.forename: Kamyar
authors.initials: K
authors.lastname: Shameli
authors.forename: Nor Azowa
authors.initials: NA
authors.lastname: Ibrahim
authors.forename: Mei Yen
authors.initials: MY
authors.lastname: Tay
authors.forename: Buong Woei
authors.initials: BW
authors.lastname: Chieng