title: DNA binding, antioxidant activity, and DNA damage protection of chiral macrocyclic Mn(III) salen complexes.
abstract: We are reporting the synthesis, characterization, and calf thymus DNA binding studies of novel chiral macrocyclic Mn(III) salen complexes S-1, R-1, S-2, and R-2. These chiral complexes showed ability to bind with DNA, where complex S-1 exhibits the highest DNA binding constant 1.20 × 10(6) M(-1). All the compounds were screened for superoxide and hydroxyl radical scavenging activities; among them, complex S-1 exhibited significant activity with IC(50) 1.36 and 2.37 μM, respectively. Further, comet assay was used to evaluate the DNA damage protection in white blood cells against the reactive oxygen species wherein complex S-1 was found effective in protecting the hydroxyl radicals mediated plasmid and white blood cells DNA damage.
authors.affiliation: Discipline of Inorganic Materials and Catalysis, Central Salt and Marine Chemicals Research Institute, Council of Scientific & Industrial Research, Bhavnagar, Gujarat, India.
authors.forename: Nirali
authors.initials: N
authors.lastname: Pandya
authors.forename: Noor-ul H
authors.initials: NU
authors.lastname: Khan
authors.forename: K Jeya
authors.initials: KJ
authors.lastname: Prathap
authors.forename: Rukhsana I
authors.initials: RI
authors.lastname: Kureshy
authors.forename: Sayed H R
authors.initials: SH
authors.lastname: Abdi
authors.forename: Sandhya
authors.initials: S
authors.lastname: Mishra
authors.forename: Hari C
authors.initials: HC
authors.lastname: Bajaj
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D004249:DNA Damage; D005029:Ethylenediamines / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000737:chemistry; D020128:Inhibitory Concentration 50; D047028:Macrocyclic Compounds / Q000737:chemistry*; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D013237:Stereoisomerism; D013481:Superoxides / Q000737:chemistry; D044366:Transition Temperature / Q000187:drug effects
chemical_list: D005029:Ethylenediamines; D016166:Free Radical Scavengers; D047028:Macrocyclic Compounds; D009942:Organometallic Compounds; D013481:Superoxides; D017665:Hydroxyl Radical; C090577:N,N'-bis(salicylideneamino)ethane-manganese(II); D004247:DNA