title: Phosphorylation-dependent SUMOylation of the transcription factor NF-E2.
abstract: Nuclear factor erythroid-derived 2 (NF-E2), a heterodimer composed of p45 and p18, is a transcriptional activator in hematopoietic progenitors. The transcriptional activity of NF-E2 is not only upregulated by SUMOylation but also stimulated by the cAMP-dependent protein kinase A (PKA). However, the relationship between SUMOylation and phosphorylation in the activation of NF-E2 is unclear. In the present studies, we have demonstrated that PKA enhances NF-E2 SUMOylation in an in vitro system using purified proteins, suggesting a possible mechanism for PKA-dependent activation of the NF-E2 transcription factor through SUMOylation.
authors.affiliation: Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Yee-Fun
authors.initials: YF
authors.lastname: Su
authors.forename: Yu-Chiau
authors.initials: YC
authors.lastname: Shyu
authors.forename: Che-Kun James
authors.initials: CK
authors.lastname: Shen
authors.forename: Jaulang
authors.initials: J
authors.lastname: Hwang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D006801:Humans; D050983:NF-E2 Transcription Factor / Q000378:metabolism*; D010766:Phosphorylation; D058207:Sumoylation
chemical_list: D050983:NF-E2 Transcription Factor; D000255:Adenosine Triphosphate; D017868:Cyclic AMP-Dependent Protein Kinases