title: Glycan profiling of endometrial cancers using lectin microarray.
abstract: Cell surface glycans change during the process of malignant transformation. To characterize and distinguish endometrial cancer and endometrium, we performed glycan profiling using an emerging modern technology, lectin microarray analysis. The three cell lines, two from endometrial cancers [well-differentiated type (G1) and poorly differentiated type (G3)] and one from normal endometrium, were successfully categorized into three independent groups by 45 lectins. Furthermore, in cancer cells, a clear difference between G1 and G3 type was observed for the glycans recognized with six lectins, Ulex europaeus agglutinin I (UEA-I), Sambucus sieboldiana agglutinin (SSA), Sambucus nigra agglutinin (SNA), Trichosanthes japonica agglutinin I (TJA-I), Amaranthus caudatus agglutinin (ACA), and Bauhinia purpurea lectin (BPL). The lectin microarray analysis using G3 type tissues demonstrated that stage I and stage III or IV were distinguished depending on signal pattern of three lectins, Dolichos biflorus agglutinin (DBA), BPL, and ACA. In addition, the analysis of the glycans on the ovarian cancer cells showed that only anticancer drug-sensitive cell lines had almost no activities to specific three lectins. Glycan profiling by the lectin microarray may be used to assess the characteristics of tumors and potentially to predict the success of chemotherapy treatment.
authors.affiliation: Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Tokyo 157-8535, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishijima
authors.forename: Masashi
authors.initials: M
authors.lastname: Toyoda
authors.forename: Mayu
authors.initials: M
authors.lastname: Yamazaki-Inoue
authors.forename: Taro
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Masaki
authors.initials: M
authors.lastname: Miyazawa
authors.forename: Toshinari
authors.initials: T
authors.lastname: Muramatsu
authors.forename: Kyoko
authors.initials: K
authors.lastname: Nakamura
authors.forename: Hisashi
authors.initials: H
authors.lastname: Narimatsu
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
authors.forename: Mikio
authors.initials: M
authors.lastname: Mikami
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D016000:Cluster Analysis; D016889:Endometrial Neoplasms / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism; D005260:Female; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D040081:Protein Array Analysis
chemical_list: D037102:Lectins; D011134:Polysaccharides