title: Comparison of Helicobacter pylori genotypes obtained from the oropharynx and stomach of the same individuals - a pilot study.
abstract: Helicobacter pylori has been recently detected in the oral cavity and           oropharynx. However, the role it plays in oral and oropharyngeal pathogenesis           remains unclear. The virulence of H. pylori strains can be distinguished according           to the virulence factors genes carried. Our research has been focused on realtime           PCR analysis of cagA and vacA genes of H. pylori strains in tonsils and tonsillar           squamous cell cancer and their comparison with H. pylori strains obtained from           the gastric mucosa of the same patients. Urea breath test (UBT) test was used           to detect a gastric H. pylori infection in 20 patients with previously proven H.           pylori in the oropharynx. Genotyping of H. pylori in gastric biopsies was performed           in patients with positive gastric infection. Out of 20 patients positive for           oropharyngeal H. pylori, 8 were positive for concurrent gastric H. pylori infection.           In 6 of them gastric biopsies were obtained. Comparison of oropharyngeal and           stomach H. pylori genotypes showed important differences. Four of 6 patients had           different H. pylori strains in the oropharynx and stomach. The differences were           found in cagA gene as well as in vacA gene. The finding of oral presence of H. pylori           without concurrent stomach infection was confirmed using UBT. The results show           that more than one H. pylori strain can be present in oropharynx and stomach in           the same patient. The oropharyngeal infection seems to be independent to the           gastric infection.
authors.affiliation: Department of Otorhinolaryngology, Charles University, Prague, Czech Republic. petr.luk@gmail.com
authors.forename: P
authors.initials: P
authors.lastname: Lukeš
authors.forename: E
authors.initials: E
authors.lastname: Pavlík
authors.forename: B
authors.initials: B
authors.lastname: Potužníková
authors.forename: J
authors.initials: J
authors.lastname: Plzák
authors.forename: E
authors.initials: E
authors.lastname: Nártová
authors.forename: J
authors.initials: J
authors.lastname: Doseděl
authors.forename: R
authors.initials: R
authors.lastname: Katra
authors.forename: I
authors.initials: I
authors.lastname: Sterzl
authors.forename: J
authors.initials: J
authors.lastname: Betka
authors.forename: J
authors.initials: J
authors.lastname: Astl
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D005838:Genotype; D016481:Helicobacter Infections; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D009960:Oropharynx / Q000382:microbiology*; D013270:Stomach / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori