title: Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures.
abstract: BACKGROUND
  Inhibition of Candida albicans on denture resins could play a significant role in preventing the development of denture stomatitis. The safety of a new dental material with antifungal properties was analyzed in this work.
  
  
  METHODS
  Poly(methyl methacrylate) [PMMA] discs and PMMA-silver nanoparticle discs were formulated, with the commercial acrylic resin, Nature-Cryl™, used as a control. Silver nanoparticles were synthesized and characterized by ultraviolet-visible spectroscopy, dispersive Raman spectroscopy, and transmission electron microscopy. The antifungal effect was assessed using a luminescent microbial cell viability assay. Biocompatibility tests were carried out using NIH-3T3 mouse embryonic fibroblasts and a Jurkat human lymphocyte cell line. Cells were cultured for 24 or 72 hours in the presence or absence of the polymer formulations and analyzed using three different tests, ie, cellular viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation by enzyme-linked immunosorbent assay BrdU, and genomic DNA damage (Comet assay). Finally, the samples were evaluated mechanically, and the polymer-bearing silver nanoparticles were analyzed microscopically to evaluate dispersion of the nanoparticles.
  
  
  RESULTS
  The results show that PMMA-silver nanoparticle discs significantly reduce adherence of C. albicans and do not affect metabolism or proliferation. They also appear not to cause genotoxic damage to cells.
  
  
  CONCLUSIONS
  The present work has developed a new biocompatible antifungal PMMA denture base material.
authors.affiliation: National School of Higher Education, School of Dentistry-Leon Unit, National Autonomus University of Mexico-UNAM, Leon, Guanajuato, Mexico.
authors.forename: Laura Susana
authors.initials: LS
authors.lastname: Acosta-Torres
authors.forename: Irasema
authors.initials: I
authors.lastname: Mendieta
authors.forename: Rosa Elvira
authors.initials: RE
authors.lastname: Nuñez-Anita
authors.forename: Marcos
authors.initials: M
authors.lastname: Cajero-Juárez
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Castaño
mesh_terms: D000180:Acrylic Resins / Q000138:chemical synthesis*; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D003764:Dental Materials / Q000138:chemical synthesis*; D003778:Dentures / Q000382:microbiology*; D006801:Humans; D019169:Jurkat Cells; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells; D019904:Polymethyl Methacrylate; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000180:Acrylic Resins; D000935:Antifungal Agents; D001672:Biocompatible Materials; D003764:Dental Materials; D012834:Silver; D019904:Polymethyl Methacrylate