title: The osteogenic differentiation improvement of human mesenchymal stem cells on titanium grafted with polyNaSS bioactive polymer.
abstract: Osseointegration of metallic implants used in orthopedic surgery requires that osteoprogenitor cells attach and adhere to the surface, then proliferate, differentiate into osteoblasts, and finally produce mineralized matrix. Because the ability of progenitor cells to attach to a scaffold surface during early stages is important in the development of new tissue structures, we developed in our laboratory, a strategy involving grafting of implants with a polymer of sodium styrene sulfonate (polyNaSS) used as a scaffold which enables human mesenchymal stem cells (hMSCs) interactions. In the present study, we investigated the cellular response of hMSCs to polyNaSS surfaces of titanium (Ti). In particular, cell proliferation, cell viability, cell differentiation, and cell spreading were evaluated. Results showed that cell proliferation and cell viability did not differ with any statistical significance between modified and unmodified Ti surfaces. Interestingly, culture of MSCs on polyNaSS surfaces resulted in a significant increase of cell spreading and cell differentiation compared with the other tested surfaces. These results suggest that titanium surface grafted with polyNaSS is a suitable scaffold for bone tissue engineering.
authors.affiliation: Université Paris 13, Sorbonne Paris Cité, UMR CNRS 7244, CSPBAT-LBPS, UFR SMBH, Bobigny, France.
authors.forename: S
authors.initials: S
authors.lastname: Oughlis
authors.forename: S
authors.initials: S
authors.lastname: Lessim
authors.forename: S
authors.initials: S
authors.lastname: Changotade
authors.forename: F
authors.initials: F
authors.lastname: Poirier
authors.forename: F
authors.initials: F
authors.lastname: Bollotte
authors.forename: J
authors.initials: J
authors.lastname: Peltzer
authors.forename: H
authors.initials: H
authors.lastname: Felgueiras
authors.forename: V
authors.initials: V
authors.lastname: Migonney
authors.forename: J J
authors.initials: JJ
authors.lastname: Lataillade
authors.forename: D
authors.initials: D
authors.lastname: Lutomski
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D010012:Osteogenesis / Q000187:drug effects*; D010710:Phosphates / Q000378:metabolism; D011137:Polystyrenes / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D010710:Phosphates; D011137:Polystyrenes; C003321:polystyrene sulfonic acid; D014025:Titanium; D000469:Alkaline Phosphatase; D002118:Calcium