title: Biosurfactant production by marine bacterial isolates from the Venezuelan Atlantic Front.
abstract: Our purpose was to detect, isolate and characterize tensioactive agents with or without emulsifying activity from marine bacterial strains present in seawater and sediment samples from the Venezuelan Atlantic Front. Biosurfactants found in cell-free supernatants from all cultures presented high surface activity as they were able to reduce the water surface tension from 72 dynes cm(-1) to values between 41.7 and 33.9 dynes cm(-1). However, high indirect CMC values were registered for the most of these compounds (51.4%-56.1% v/v). Culture supernatants from S3 and S29 strains showed highest emulsifying activity in the dispersion assay with diesel oil (absorbance 1.06 and 1.18, respectively), while supernatant from the S10 strain induced the most stable oil-in-water emulsions with 16° and 25°API crude oils. Only culture supernatant from S3 strain was able to produce stable oil-in water emulsions with diesel oil and both type of crude oils.
authors.affiliation: Dpto. Biología de Organismos, Universidad Simón Bolívar, Baruta, Apartado 89000, Caracas 1080, Venezuela. lorelei.bozo@gmail.com
authors.forename: Lorelei
authors.initials: L
authors.lastname: Bozo-Hurtado
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Rocha
authors.forename: Raydav
authors.initials: R
authors.lastname: Malavé
authors.forename: Paula
authors.initials: P
authors.lastname: Suárez
mesh_terms: D001267:Atlantic Ocean; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D010578:Petroleum / Q000032:analysis / Q000378:metabolism; D012623:Seawater / Q000737:chemistry / Q000382:microbiology*; D013500:Surface Tension; D013501:Surface-Active Agents / Q000378:metabolism*; D014687:Venezuela; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism
chemical_list: D010578:Petroleum; D013501:Surface-Active Agents; D014874:Water Pollutants, Chemical