title: Complete genetic characterization of human G2P[6] and G3P[6] rotavirus strains.
abstract: During the 2008-2009 rotavirus season, 10 G3P[6] rotavirus strains were isolated for the first time in Belgium, while an outbreak of G2P[6] strains occurred in the USA in 2005-2006. Partial sequencing of the 11 genome segments of the 10 Belgian G3P[6] strains revealed a clonal origin. Two of these strains, and a G2P[6] strain representative of the American outbreak, were selected and sequenced completely to analyze their evolutionary relationships. Genetic analysis revealed that all strains possessed a DS-1-like genotype constellation. The 2 Belgian G3P[6] strains showed >99% sequence identity at the nucleotide level and the American G2P[6] strain was phylogenetically closely related to the Belgian P[6] strains. These data suggest that reassortment(s) involving VP7 occurred recently, and that the prevalence of DS-1-like P[6] rotavirus strains need to be closely monitored because the currently licensed RVA vaccines contain neither the P[6] genotype nor strains with a complete human DS-1 genotype constellation.
authors.affiliation: Laboratory of Clinical Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, Minderbroedersstraat 10, BE-3000 Leuven, Belgium.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Heylen
authors.forename: Mark
authors.initials: M
authors.lastname: Zeller
authors.forename: Max
authors.initials: M
authors.lastname: Ciarlet
authors.forename: Sarah
authors.initials: S
authors.lastname: De Coster
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
authors.forename: Jelle
authors.initials: J
authors.lastname: Matthijnssens
mesh_terms: D001530:Belgium; D004196:Disease Outbreaks; D005814:Genes, Viral; D016679:Genome, Viral; D005838:Genotype; D049674:History, 21st Century; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000266:history; D014481:United States