title: Prognostic value of rapid test for diagnosis of dengue in Nepalese patients during 2010 epidemic.
abstract: BACKGROUND
  Dengue is an emerging vector borne disease in Nepal and rapid diagnostic test is important for early diagnosis of the disease.
  
  
  OBJECTIVE
  The aim of the study was to evaluate the diagnostic accuracy of commonly used rapid immunochromatographic test kit in Nepal during 2010 dengue epidemic and to assess disease burden of dengue.
  
  
  METHODS
  A total of 131 acute and nonacute serum samples were collected during recent epidemic of dengue in 2010 from clinically suspected Nepalese patients of different hospitals. Rapid immunochromatographic test kit was used for early diagnosis and enzyme immunosorbent was chosen as a reference assay.
  
  
  RESULTS
  The sensitivity and specificity of rapid test was 70% and 76.54% respectively whereas the prevalence of the disease was 38.17%. The odds ratio for males was 1.8 however; the association with the disease was statistically not significant.
  
  
  CONCLUSIONS
  The diagnostic accuracy of rapid immunochromatographic test for dengue diagnosis was low (k=0.46). So, it should be substituted by highly sensitive test device for prompt diagnosis and health personnel should consider appropriate timing of sample collection for better performance of rapid test.
authors.affiliation: Everest International Clinic and Research Centre, Kathmandu, Nepal. rameshpun2007@gmail.com
authors.forename: R
authors.initials: R
authors.lastname: Pun
authors.forename: Y
authors.initials: Y
authors.lastname: Shah
authors.forename: G P
authors.initials: GP
authors.lastname: Gupta
authors.forename: S P
authors.initials: SP
authors.lastname: Sherchand
authors.forename: B D
authors.initials: BD
authors.lastname: Pandey
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002675:Child, Preschool; D002846:Chromatography, Affinity; D003430:Cross-Sectional Studies; D003715:Dengue / Q000175:diagnosis* / Q000453:epidemiology*; D004797:Enzyme-Linked Immunosorbent Assay; D058872:Epidemics; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D008297:Male; D008875:Middle Aged; D009390:Nepal / Q000453:epidemiology; D011379:Prognosis; D012680:Sensitivity and Specificity; D013997:Time Factors; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M