title: A small predatory core genome in the divergent marine Bacteriovorax marinus SJ and the terrestrial Bdellovibrio bacteriovorus.
abstract: Bacteriovorax marinus SJ is a predatory delta-proteobacterium isolated from a marine environment. The genome sequence of this strain provides an interesting contrast to that of the terrestrial predatory bacterium Bdellovibrio bacteriovorus HD100. Based on their predatory lifestyle, Bacteriovorax were originally designated as members of the genus Bdellovibrio but subsequently were re-assigned to a new genus and family based on genetic and phenotypic differences. B. marinus attaches to gram-negative bacteria, penetrates through the cell wall to form a bdelloplast, in which it replicates, as shown using microscopy. Bacteriovorax is distinct, as it shares only 30% of its gene products with its closest sequenced relatives. Remarkably, 34% of predicted genes over 500 nt in length were completely unique with no significant matches in the databases. As expected, Bacteriovorax shares several characteristic loci with the other delta-proteobacteria. A geneset shared between Bacteriovorax and Bdellovibrio that is not conserved among other delta-proteobacteria such as Myxobacteria (which destroy prey bacteria externally via lysis), or the non-predatory Desulfo-bacteria and Geobacter species was identified. These 291 gene orthologues common to both Bacteriovorax and Bdellovibrio may be the key indicators of host-interaction predatory-specific processes required for prey entry. The locus from Bdellovibrio bacteriovorus is implicated in the switch from predatory to prey/host-independent growth. Although the locus is conserved in B. marinus, the sequence has only limited similarity. The results of this study advance understanding of both the similarities and differences between Bdellovibrio and Bacteriovorax and confirm the distant relationship between the two and their separation into different families.
authors.affiliation: Department of Bioinformatics, The Genome Analysis Centre, Norwich Research Park, Norwich, UK. lisa.crossman@bbsrc.ac.uk
authors.forename: Lisa C
authors.initials: LC
authors.lastname: Crossman
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.forename: Ana-M
authors.initials: AM
authors.lastname: Cerdeño-Tárraga
authors.forename: Karen
authors.initials: K
authors.lastname: Brooks
authors.forename: Michael A
authors.initials: MA
authors.lastname: Quail
authors.forename: Silvia A
authors.initials: SA
authors.lastname: Pineiro
authors.forename: Laura
authors.initials: L
authors.lastname: Hobley
authors.forename: R Elizabeth
authors.initials: RE
authors.lastname: Sockett
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Bentley
authors.forename: Julian
authors.initials: J
authors.lastname: Parkhill
authors.forename: Henry N
authors.initials: HN
authors.lastname: Williams
authors.forename: O Colin
authors.initials: OC
authors.lastname: Stine
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001501:Bdellovibrio / Q000145:classification / Q000235:genetics* / Q000502:physiology; D020564:Deltaproteobacteria / Q000145:classification / Q000235:genetics* / Q000502:physiology; D020387:Food Chain; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids; D012623:Seawater / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins