title: Analysis of ThiC variants in the context of the metabolic network of Salmonella enterica.
abstract: In bacteria, the 4-amino-hydroxymethyl-2-methylpyrimidine (HMP) moiety of thiamine is synthesized from 5-aminoimidazole ribotide (AIR), a branch point metabolite of purine and thiamine biosynthesis. ThiC is a member of the radical S-adenosylmethionine (AdoMet) superfamily and catalyzes the complex chemical rearrangement of AIR to HMP-P. As reconstituted in vitro, the ThiC reaction requires AdoMet, AIR, and reductant. This study analyzed variants of ThiC in vivo and in vitro to probe the metabolic network surrounding AIR in Salmonella enterica. Several variants of ThiC that required metabolic perturbations to function in vivo were biochemically characterized in vitro. Results presented herein indicate that the subtleties of the metabolic network have not been captured in the current reconstitution of the ThiC reaction.
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, USA.
authors.forename: Lauren D
authors.initials: LD
authors.lastname: Palmer
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Dougherty
authors.forename: Diana M
authors.initials: DM
authors.lastname: Downs
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D014644:Genetic Variation; D008715:Methionine; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009154:Mutation; D010205:Pantothenic Acid / Q000378:metabolism; D012486:Salmonella typhimurium / Q000378:metabolism*; D013831:Thiamine / Q000096:biosynthesis / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; C105797:ThiC protein, Bacteria; D010205:Pantothenic Acid; D008715:Methionine; D013831:Thiamine