title: Autologous bone marrow stromal cells loaded onto porous gelatin scaffolds containing Drynaria fortunei extract for bone repair.
abstract: GGT-GSB composite was prepared by mixing a biodegradable GGT composite containing genipin-crosslinked gelatin and β-tricalcium phosphate with Gu-Sui-Bu extract (GSB) (Drynaria fortunei (Kunze) J. Sm.), a traditional Chinese medicine. Then, porous GGT and GGT-GSB scaffolds were fabricated using a salt-leaching method. The GGT and GGT-GSB scaffolds thus obtained had a macroporous structure and high porosity. Rabbit bone marrow stromal cells (BMSCs) were seeded onto GGT and GGT-GSB scaffolds. The biological response of rabbit calvarial bone to these scaffolds was considered to evaluate the potential of the scaffolds for use in bone tissue engineering. After 8 weeks of implantation, each scaffold induced new bone formation at a cranial bone defect, as was verified by X-ray microradiography. The BMSC-seeded GGT-GSB scaffolds induced more new bone formation than the BMSC-seeded GGT and acellular scaffolds. These observations suggest that an autologous BMSCs-seeded porous GGT-GSB scaffold can be adopted in bone engineering in vivo and has great potential for regenerating defective bone tissue.
authors.affiliation: Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan.
authors.forename: Kuo-Yu
authors.initials: KY
authors.lastname: Chen
authors.forename: Guo-Chung
authors.initials: GC
authors.lastname: Dong
authors.forename: Chin-Yin
authors.initials: CY
authors.lastname: Hsu
authors.forename: Yueh-Sheng
authors.initials: YS
authors.lastname: Chen
authors.forename: Chun-Hsu
authors.initials: CH
authors.lastname: Yao
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D016026:Bone Marrow Transplantation; D001861:Bone Regeneration / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D029621:Polypodiaceae / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D012886:Skull / Q000293:injuries*; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D013997:Time Factors; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds; D014182:Transplantation, Autologous
chemical_list: D004365:Drugs, Chinese Herbal; D005780:Gelatin