title: Real-time analysis and direct observations of different superoxide dismutase (SOD1) molecules bindings to aggregates in temporal evolution step.
abstract: The misfolding and intracellular aggregation of Cu-Zn superoxide dismutase (SOD1) is pathologically key feature of amyotrophic lateral sclerosis (ALS). Although details of the mechanisms continue to be unclear, there are key steps in the possible pathway to the development of ALS. This study focuses on interactions between different SOD1 molecules (A4V apo/holo, and WT apo/holo) and homogeneous aggregates in the temporal evolution step, and a determination of whether any of the SOD1 molecules are reactive to the aggregates with the extent of binding, as determined by surface plasmon resonance (SPR) measurements. Using a kinetic binding model, the association constant of A4V apo was found to be three times larger than that for the WT apo species. Differences in the extent of the interactions were also simultaneously measured and visualized by means of SPR imaging techniques. The SPR-based approach suggests direct correlation between SPR signal and the extent of molecular binding, which can identify the significant contributors to the formation of macroaggregates of SOD1 in the temporal evolution step.
authors.affiliation: Department of Applied Bioscience, CHA University, Gyeonggi-do 463-863, Republic of Korea. hongsr@cha.ac.kr
authors.forename: Surin
authors.initials: S
authors.lastname: Hong
authors.forename: Suseung
authors.initials: S
authors.lastname: Lee
authors.forename: Inhee
authors.initials: I
authors.lastname: Choi
authors.forename: Young In
authors.initials: YI
authors.lastname: Yang
authors.forename: Taewook
authors.initials: T
authors.lastname: Kang
authors.forename: Jongheop
authors.initials: J
authors.lastname: Yi
mesh_terms: D000465:Algorithms; D002942:Circular Dichroism; D003199:Computer Systems; D007700:Kinetics; D046210:Microfluidic Analytical Techniques; D011485:Protein Binding; D017510:Protein Folding; D013482:Superoxide Dismutase / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D000072105:Superoxide Dismutase-1; D020349:Surface Plasmon Resonance
chemical_list: D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1