title: Lipopolysaccharide induces endothelial cell apoptosis via activation of Na(+)/H(+) exchanger 1 and calpain-dependent degradation of Bcl-2.
abstract: The calcium-dependent protease calpain is involved in lipopolysaccharide (LPS)-induced endothelial injury. The activation of Na(+)/H(+) exchanger (NHE) is responsible to increase intracellular Ca(2+) (Ca(i)(2+)) in cardiovascular diseases. Here we hypothesized that activation of NHE mediates LPS-induced endothelial cell apoptosis via calcium-dependent calpain pathway. Our results revealed that LPS-induced increases in NHE activity are dependent on NHE1 in human umbilical vein endothelial cells (HUVECs). Treatment of HUVECs with LPS increased the NHE1 activity in a time-dependent manner associated with the increased Ca(i)(2+), which resulted in enhanced calpain activity as well as HUVECs apoptosis via NHE1-dependent degradation of Bcl-2.
authors.affiliation: Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Zhao
authors.forename: Guimei
authors.initials: G
authors.lastname: Cui
authors.forename: Nana
authors.initials: N
authors.lastname: Zhang
authors.forename: Zengshan
authors.initials: Z
authors.lastname: Liu
authors.forename: Wanchun
authors.initials: W
authors.lastname: Sun
authors.forename: Qisheng
authors.initials: Q
authors.lastname: Peng
mesh_terms: D017209:Apoptosis / Q000276:immunology*; D002118:Calcium / Q000378:metabolism; D002154:Calpain / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D059748:Proteolysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers / Q000096:biosynthesis*
chemical_list: D027682:Cation Transport Proteins; D008070:Lipopolysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; C490998:SLC9A1 protein, human; D000074082:Sodium-Hydrogen Exchanger 1; D017923:Sodium-Hydrogen Exchangers; D002154:Calpain; D002118:Calcium