title: Iristectorigenin B isolated from Belamcanda chinensis is a liver X receptor modulator that increases ABCA1 and ABCG1 expression in macrophage RAW 264.7 cells.
abstract: A novel liver X receptor (LXR) modulator, iristectorigenin B isolated from Belamcanda chinensis, stimulated the transcriptional activity of both LXR-α and LXR-β. In macrophages, iristectorigenin B suppressed cholesterol accumulation in a dose-dependent manner and induced the transcriptional activation of LXR-α/-β-responsive genes, ATP-binding cassette transporters A1 and G1. It did not induce hepatic lipid accumulation nor the expression of the lipogenesis genes sterol regulatory element-binding protein-1c, fatty acid synthase, and stearoyl-CoA desaturase-1. Iristectorigenin B thus is a dual-LXR agonist that regulates the expression of key genes in cholesterol homeostasis in macrophage cells without inducing hepatic lipid accumulation.
authors.affiliation: Department of Biotechnology, Graduate School of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
authors.forename: Hee-jin
authors.initials: HJ
authors.lastname: Jun
authors.forename: Minh-Hien
authors.initials: MH
authors.lastname: Hoang
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Lee
authors.forename: Jia
authors.initials: J
authors.lastname: Yaoyao
authors.forename: Ji-Hae
authors.initials: JH
authors.lastname: Lee
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Lee
authors.forename: Hak-Ju
authors.initials: HJ
authors.lastname: Lee
authors.forename: Woo-Duck
authors.initials: WD
authors.lastname: Seo
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis*; D000818:Animals; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects*; D027621:Iridaceae / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008074:Lipoproteins / Q000096:biosynthesis*; D000071518:Liver X Receptors; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D057093:Orphan Nuclear Receptors / Q000819:agonists*
chemical_list: C487540:ABCG1 protein, mouse; D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; D007529:Isoflavones; D008074:Lipoproteins; D000071518:Liver X Receptors; C000605214:Nr1h3 protein, mouse; D057093:Orphan Nuclear Receptors; C120039:tectorigenin