title: Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR.
abstract: Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
authors.affiliation: Applied Biotechnology Division, National Veterinary Research Institute, PMB 1, Vom, Plateau State, Nigeria. pamluka08@gmail.com
authors.forename: Pam Dachung
authors.initials: PD
authors.lastname: Luka
authors.forename: Chrisostom
authors.initials: C
authors.lastname: Ayebazibwe
authors.forename: David
authors.initials: D
authors.lastname: Shamaki
authors.forename: Frank Norbert
authors.initials: FN
authors.lastname: Mwiine
authors.forename: Joseph
authors.initials: J
authors.lastname: Erume
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000032:analysis; D005123:Eye / Q000821:virology; D015511:Goat Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D006041:Goats; D006197:Hair / Q000821:virology; D009666:Nose / Q000821:virology; D009698:Nucleoproteins / Q000032:analysis; D029021:Peste-des-Petits-Ruminants / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000302:isolation & purification*; D010858:Pigmentation; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D014454:Uganda / Q000453:epidemiology
chemical_list: D017931:DNA Primers; D009698:Nucleoproteins; D012367:RNA, Viral