title: PAirwise Sequence Comparison (PASC) and its application in the classification of filoviruses.
abstract: PAirwise Sequence Comparison (PASC) is a tool that uses genome sequence similarity to help with virus classification. The PASC tool at NCBI uses two methods: local alignment based on BLAST and global alignment based on Needleman-Wunsch algorithm. It works for complete genomes of viruses of several families/groups, and for the family of Filoviridae, it currently includes 52 complete genomes available in GenBank. It has been shown that BLAST-based alignment approach works better for filoviruses, and therefore is recommended for establishing taxon demarcations criteria. When more genome sequences with high divergence become available, these demarcation will most likely become more precise. The tool can compare new genome sequences of filoviruses with the ones already in the database, and propose their taxonomic classification.
authors.affiliation: National Center for Biotechnology Information, National Institutes of Health, Bethesda, MD 20892, USA. bao@ncbi.nlm.nih.gov
authors.forename: Yiming
authors.initials: Y
authors.lastname: Bao
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Chetvernin
authors.forename: Tatiana
authors.initials: T
authors.lastname: Tatusova
mesh_terms: D001483:Base Sequence; D030561:Databases, Nucleic Acid; D016563:Filoviridae / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment / Q000295:instrumentation / Q000379:methods*; D017422:Sequence Analysis, DNA; D012984:Software