title: Molecular cloning and characterization of the anti-obesity gene adipose in pig.
abstract: Obesity has become an epidemic health problem characterized by aberrant energy metabolism. As the major player in energy homeostasis, adipose tissue has a decisive role in the development of obesity. Many genes involved in adipogenesis are also correlated with obesity. Adipose (Adp) has been established as an anti-obesity gene to repress adipogenesis and fat accumulation in mice, which inhibits the transcriptional activity of PPARγ by forming a chromatin remodeling complex with histones and HDAC3. Here, we reported the cloning and characterization of the pig Adp gene. Pig Adp cDNA had an ORF of 2034 nucleotides and was highly conserved among various species. Genomic sequence analysis indicated that pig Adp gene contains 16 exons and 15 introns, spanning more than 60kb on chromosome 6q21-24. The expression of pig Adp was high in testis, lung, kidney and adipose tissues, and relatively low in skeletal muscle. Bioinformatic analysis of 5'-flanking region of Adp has identified several potential binding sites for pivotal transcriptional factors related to both adipocyte differentiation and inflammation, highlighting the significance of Adp in energy metabolism. We have confirmed that KLF6, a positive regulator of adipogenesis, can enhance the promoter activity of Adp and up-regulate its mRNA expression. Taken together, our results would be helpful for further study of Adp regulation in the process of fat accumulation.
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, PR China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Wu
authors.forename: Qinqiang
authors.initials: Q
authors.lastname: Long
authors.forename: Bin
authors.initials: B
authors.lastname: Feng
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Zhu
authors.forename: Zifeng
authors.initials: Z
authors.lastname: Zheng
authors.forename: Sumin
authors.initials: S
authors.lastname: Gao
authors.forename: Mingju
authors.initials: M
authors.lastname: Gao
authors.forename: Li
authors.initials: L
authors.lastname: Gan
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Zaiqing
authors.initials: Z
authors.lastname: Yang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004734:Energy Metabolism / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D023421:Models, Animal; D008969:Molecular Sequence Data; D009765:Obesity / Q000235:genetics* / Q000378:metabolism / Q000517:prevention & control*; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014018:Tissue Distribution; D014157:Transcription Factors / Q000378:metabolism; D043743:Ubiquitin-Protein Ligase Complexes
chemical_list: D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C527990:Wdtc1 protein, mouse; D043743:Ubiquitin-Protein Ligase Complexes