title: [Study on molecular characteristics of four components contained in Hedysari Radix polysaccharide by gel permeation chromatography-multiangle laser light scattering technology (GPC-MALLS)].
abstract: OBJECTIVE
  To determine such molecular characteristic parameters as absolute molecular weight, molecular weight distribution, root-mean-square turning radius (Rg) and polydispersity index (Mw/Mn) of four components contained in Hedysari Radix polysaccharide 3 (HPS-3) and map weight-average molecular weight (Mw) with root-mean-square turning radius (Rg), in order to calculate conformations of the four components at solution state.
  
  
  METHODS
  The gel permeation chromatography-multi angle laser light scatting (GPC-MALLS) was adopted, with 0.1 mol x L(-1) NaNO3 contained 0.02% NaN3 as the mobilephase, Ultrahydrogel 1000 connected in series with Ultrahydrogel500.
  
  
  RESULTS
  Among the four components of HPS-3, HPS-3-C showed the highest weight average molecular weight of 1.986 x 10(5) g x mol(-1), followed by HPS-3-B 1.113 x 10(5) g x mol(-1) and HPS-3-D 8.457 x 10(4) g x mol(-1) HPS-3-A showed the lowest weight average molecular weight of 1. 223 x 10(4) g x mol(-1) but the highest square radius of gyration, that is 55.5 nm. HPS-3-D had the widest range of molecular weight distribution in four components, with the polydispersity index (Mw/Mn) of 2.543. In the mobile phase, HPS-3-A was globular structure, HPS-3-C was random coil, HPS-3-B and HPS-3-D were both highly branched structure.
  
  
  CONCLUSIONS
  The results provided necessary basis for further studies on molecular characteristics of the four components contained in HPS-3 and their relationship with bioactivity.
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
authors.forename: Tongqiang
authors.initials: T
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Adilbekov
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Shen
authors.forename: Yikai
authors.initials: Y
authors.lastname: Shi
authors.forename: Fangdi
authors.initials: F
authors.lastname: Hu
authors.forename: Shilan
authors.initials: S
authors.lastname: Feng
mesh_terms: D002850:Chromatography, Gel; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007834:Lasers; D008027:Light; D011134:Polysaccharides / Q000737:chemistry*; D012542:Scattering, Radiation
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides