title: Analysis of short-chain aliphatic amines in food and water samples using a near infrared cyanine 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium with CE-LIF detection.
abstract: Precolumn derivatization of six short-chain aliphatic amines by a near-infrared dye, 1-(ε-succinimydyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'- disulfonate potassium (MeCy5-OSu), followed by MEKC-CE-LIF detection has been developed as a method for the determination of aliphatic amines in environmental water and food. Optimum derivatization was operated nicely in pH 9.0 borate buffer at 20°C for 30 min. Well separated peaks were observed with a pH 9.5 BGE containing 10 mmol L⁻¹ phosphoric acid, 20 mmol L⁻¹ SDS, and 7% methanol buffered with 1.0 mol L⁻¹ NaOH. The separation procedure was rapidly achieved within 11 min and the matrix interferences could be effectively eliminated. A linear calibration graph was obtained for 5-200 nmol L⁻¹ analytes with a correlation coefficient in the range 0.9933-0.9995 for amines. This method was successfully utilized to determine aliphatic amines in lake, sewage water, and red wine with recoveries ranging from 96.4 to 105% and the RSDs ranging from 0.9 to 2.9%. Near-infrared, LIF-detector-compatible MeCy5-OSu was proved suitable for the accurate, sensitive, and rapid separation and determination of aliphatic amines in water and food samples.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine-Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, P. R. China.
authors.forename: Ni-Na
authors.initials: NN
authors.lastname: Fu
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
mesh_terms: D000588:Amines / Q000032:analysis*; D002232:Carbocyanines / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D060106:Lakes / Q000737:chemistry; D016014:Linear Models; D000432:Methanol / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012722:Sewage / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D000588:Amines; D002232:Carbocyanines; D005456:Fluorescent Dyes; D012722:Sewage; D014874:Water Pollutants, Chemical; C519607:cyanine-1-(epsilon-succinimidyl-hexanoate)-1'-methyl-3,3,3',3'-tetramethyl-indocarbocyanine-5,5'-disulfonate potassium; D012967:Sodium Dodecyl Sulfate; D000432:Methanol