title: Luteolin prevents solar radiation-induced matrix metalloproteinase-1 activation in human fibroblasts: a role for p38 mitogen-activated protein kinase and interleukin-20 released from keratinocytes.
abstract: Human skin is continuously exposed to solar radiation, which can result in photoaging, a process involving both dermal and, to a lesser extent, epidermal structures. Previously, we have shown that the flavonoid luteolin protects the epidermis from ultraviolet (UV)-induced damage by a combination of UV-absorbing, antioxidant, and antiinflammatory properties. The aim of the present study was to determine direct and indirect effects of luteolin on dermal fibroblasts as major targets of photoaging. Stimulation of fibroblasts with UVA light or the proinflammatory cytokine interleukin-20 (IL-20) is associated with wrinkled skin, increased IL-6 secretion, matrix metalloproteinase (MMP-1) expression, and hyaluronidase activity. All of these targets were inhibited by luteolin via interference with the p38 mitogen-activated protein kinase (MAPK) pathway. Next, we assessed the role of conditioned supernatants from keratinocytes irradiated with solar-simulated radiation (SSR) on nonirradiated dermal fibroblasts. In keratinocytes, luteolin inhibited SSR-induced production of IL-20, also via interference with the p38 MAPK pathway. Similarly, keratinocyte supernatant-induced IL-6 and MMP-1 expression in fibroblasts was reduced by pretreatment of keratinocytes with luteolin. Finally, these results were confirmed ex vivo on skin explants treated with luteolin before UV irradiation. Our results suggest that SSR-mediated production of soluble factors in keratinocytes is modulated by luteolin and may attenuate photoaging in dermal fibroblasts.
authors.affiliation: Competence Center skintegral, Department of Dermatology, University Freiburg Medical Center, Germany. ute.woelfle@uniklinik-freiburg.de
authors.forename: Ute
authors.initials: U
authors.lastname: Wölfle
authors.forename: Anja
authors.initials: A
authors.lastname: Heinemann
authors.forename: Philipp R
authors.initials: PR
authors.lastname: Esser
authors.forename: Birgit
authors.initials: B
authors.lastname: Haarhaus
authors.forename: Stefan F
authors.initials: SF
authors.lastname: Martin
authors.forename: Christoph M
authors.initials: CM
authors.lastname: Schempp
mesh_terms: D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007378:Interleukins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015603:Keratinocytes / Q000201:enzymology / Q000378:metabolism*; D047311:Luteolin / Q000494:pharmacology*; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D012993:Solar Energy; D014466:Ultraviolet Rays; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D007378:Interleukins; D048051:p38 Mitogen-Activated Protein Kinases; D020781:Matrix Metalloproteinase 1; D047311:Luteolin; C422042:interleukin 20