title: [Genotypic characterization of toxigenic Escherichia coli isolated from pigs with postweaning diarrhea (PWD) and edema disease (ED)].
abstract: The purpose of this work was to characterize 47 Escherichia coli strains isolated from 32 pigs diagnosed with postweaning diarrhea and three pigs with edema disease by PCR. Forty two (95.5 %) of the strains isolated from diarrheic pigs were characterized as enterotoxigenic E. coli (ETEC) and 2 (4.5 %) as Shiga toxin-producing E. coli (STEC). Fourteen (33.3 %) ETEC strains were positive for est/estII/fedA genes. The most complex genotype was eltA/estI/faeG/aidA. Strains isolated from pigs with ED were classified as porcine STEC and were stx2e/aidA carriers. Eleven (25 %) strains carried the gene encoding adhesin protein AIDA-I. However, genes coding for F5, F6, F41, intimin and Paa were not detected. The development of vaccines generating antibodies against prevalent E. coli adhesins in Argentina could be useful for the prevention of PWD and ED.
authors.affiliation: Catedra de Microbiología, Facultad de Ciencias Veterinarias, UNLP, Calle 60 y 118, (1900) La Plata, Pcia. de Buenos Aires, Argentina. fmoredo@fcv.unlp.edu.ar
authors.forename: Fabiana A
authors.initials: FA
authors.lastname: Moredo
authors.forename: Javier A
authors.initials: JA
authors.lastname: Cappuccio
authors.forename: Lucas
authors.initials: L
authors.lastname: Insarralde
authors.forename: Carlos J
authors.initials: CJ
authors.lastname: Perfumo
authors.forename: María A
authors.initials: MA
authors.lastname: Quiroga
authors.forename: Gerardo A
authors.initials: GA
authors.lastname: Leotta
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004196:Disease Outbreaks; D004488:Edema Disease of Swine / Q000453:epidemiology / Q000382:microbiology*; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D004768:Enterotoxins / Q000235:genetics; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D005838:Genotype; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*; D014886:Weaning
chemical_list: D018830:Adhesins, Escherichia coli; D004768:Enterotoxins; D029968:Escherichia coli Proteins