title: Role of CD61+ cells in thrombocytopenia of dengue patients.
abstract: Although hematological disorders with salient features of thrombocytopenia have been well documented in dengue patients, the role of CD61-expressing platelets and the megakaryocytic cell lineage in the pathogenesis of dengue virus (DENV) infection remains largely unexplored. A prospective observational study was performed using blood samples and PBMCs from dengue-confirmed patients, as well as from rhesus monkeys (RM) experimentally infected with DENV. Immunohistochemical staining and FACS techniques were applied to evaluate the frequencies of CD61(+) cells that contained DENV antigen. Highly enriched population of CD61(+) cells was also isolated from acute DENV-infected RM and assayed for DENV RNA by quantitative RT-PCR. Results revealed that DENV antigen was found in small vesicles of varying size, and more frequently in anucleated cells associated with platelets in dengue patients. The DENV antigen-containing cells were CD61(+) and appeared to share characteristics of megakaryocytes. Kinetic profiles of CD61(+) cells from DENV-infected RM revealed a transient increase in CD61(+)CD62P(+) cells early after DENV infection. DENV RNA in a highly enriched population of CD61(+) cells from the infected RM was observed during acute stage. Our results indicate that virus containing CD61(+) cells may be directly linked to the platelet dysfunction and low platelet count characteristics of dengue patients.
authors.affiliation: Department of Pathology and Laboratory Medicine, Emory Vaccine Center, Emory University School of Medicine, Dental School Building, Room 429, 1462 Clifton Road, Atlanta, GA 30322, USA.
authors.forename: Sansanee
authors.initials: S
authors.lastname: Noisakran
authors.forename: Nattawat
authors.initials: N
authors.lastname: Onlamoon
authors.forename: Kovit
authors.initials: K
authors.lastname: Pattanapanyasat
authors.forename: Hui-Mien
authors.initials: HM
authors.lastname: Hsiao
authors.forename: Pucharee
authors.initials: P
authors.lastname: Songprakhon
authors.forename: Nasikarn
authors.initials: N
authors.lastname: Angkasekwinai
authors.forename: Kulkanya
authors.initials: K
authors.lastname: Chokephaibulkit
authors.forename: Francois
authors.initials: F
authors.lastname: Villinger
authors.forename: Aftab A
authors.initials: AA
authors.lastname: Ansari
authors.forename: Guey Chuen
authors.initials: GC
authors.lastname: Perng
mesh_terms: D000208:Acute Disease; D000818:Animals; D000956:Antigens, Viral / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism / Q000821:virology; D003715:Dengue / Q000097:blood / Q000150:complications; D003716:Dengue Virus / Q000378:metabolism*; D005260:Female; D006801:Humans; D039661:Integrin beta3 / Q000097:blood*; D008253:Macaca mulatta; D008297:Male; D008533:Megakaryocytes / Q000378:metabolism / Q000821:virology; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood; D013921:Thrombocytopenia / Q000097:blood / Q000209:etiology / Q000821:virology
chemical_list: D000956:Antigens, Viral; C061248:ITGB3 protein, human; D039661:Integrin beta3; D012367:RNA, Viral