title: Structure and biosynthesis of the antibiotic bottromycin D.
abstract: Drug resistant infectious diseases are quickly becoming a global health crisis. While Streptomyces spp. have been a major source of antibiotics over the past 50 years, efficient methods are needed to identify new antibiotics and greatly improve the rate of discovery. LCMS-based metabolomics were applied to analyze extracts of 50 Streptomyes spp. Using this methodology, we discovered bottromycin D and used whole genome sequencing to determine its biosynthesis by a ribosomal pathway.
authors.affiliation: Pharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Hou
authors.forename: Ma Diarey B
authors.initials: MD
authors.lastname: Tianero
authors.forename: Jason C
authors.initials: JC
authors.lastname: Kwan
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Wyche
authors.forename: Cole R
authors.initials: CR
authors.lastname: Michel
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Ellis
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Vazquez-Rivera
authors.forename: Doug R
authors.initials: DR
authors.lastname: Braun
authors.forename: Warren E
authors.initials: WE
authors.lastname: Rose
authors.forename: Eric W
authors.initials: EW
authors.lastname: Schmidt
authors.forename: Tim S
authors.initials: TS
authors.lastname: Bugni
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry*; D015394:Molecular Structure; D005810:Multigene Family; D010456:Peptides, Cyclic / Q000096:biosynthesis / Q000737:chemistry; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D010456:Peptides, Cyclic; C024712:bottromycin