title: Protective effects of ebselen on sodium-selenite-induced experimental cataract in rats.
abstract: OBJECTIVE
  To determine whether ebselen has a protective effect or antioxidative potential in a sodium-selenite-induced experimental cataract model.
  
  
  METHODS
  Fırat University, Elazığ, Turkey.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Twenty-one Sprague-Dawley rat pups were randomly divided into a control group, a sodium-selenite-induced-cataract group, and an ebselen-treated group; each group contained 7 rat pups. Rats in the control group received dimethyl sulfoxide (DMSO) intraperitoneally only and rats in the sodium-selenite-induced-cataract group received 30 nmol/g body weight sodium selenite subcutaneously and DMSO intraperitoneally 10 days postpartum. Rats in the ebselen group received 30 nmol/g body weight sodium selenite subcutaneously 10 days postpartum and were treated with 5 mg/kg body weight ebselen once a day for 4 consecutive days. Cataract development was assessed weekly for 3 weeks by slitlamp examination and graded using a scale. Reduced glutathione (GSH), total nitrite, and malondialdehyde (MDA) levels in lens supernatants were measured at the end of 3 weeks.
  
  
  RESULTS
  In the control group, all lenses were clear. In the ebselen-treated group, the mean cataract stage was significantly lower than in the sodium-selenite-induced-cataract group (P = .022). The GSH levels were significantly lower in the sodium-selenite-induced-cataract group than in the control and ebselen groups (P < .001). The MDA levels were lower in the ebselen group than in the sodium-selenite-induced-cataract group (P < .001). The mean total nitrite level was significantly lower in the sodium-selenite-induced-cataract group than in the ebselen group (P = .001).
  
  
  CONCLUSIONS
  Ebselen had a protective effect on cataract development in a sodium-selenite-induced experimental model. The protective effect of ebselen appears to be due to inhibition of oxidative stress.
  
  
  BACKGROUND
  No author has a financial or proprietary interest in any material or method mentioned.
authors.affiliation: Department of Ophthalmology, School of Medicine, Fırat University, Elazığ, Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Aydemir
authors.forename: Mete
authors.initials: M
authors.lastname: Güler
authors.forename: Mehmet Kaan
authors.initials: MK
authors.lastname: Kaya
authors.forename: Nurettin
authors.initials: N
authors.lastname: Deniz
authors.forename: Bilal
authors.initials: B
authors.lastname: Üstündağ
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001393:Azoles / Q000627:therapeutic use*; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D054833:Isoindoles; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D016566:Organoselenium Compounds / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D018038:Sodium Selenite / Q000633:toxicity
chemical_list: D000975:Antioxidants; D001393:Azoles; D054833:Isoindoles; D009573:Nitrites; D016566:Organoselenium Compounds; C042986:ebselen; D008315:Malondialdehyde; D005978:Glutathione; D018038:Sodium Selenite