title: Murine skin and vaginal mucosa are similarly susceptible to infection by pseudovirions of different papillomavirus classifications and species.
abstract: Depending upon viral genotype, productive papillomavirus infection and disease display preferential tropism for cutaneous or mucosal stratified squamous epithelia, although the mechanisms are unclear. To investigate papillomavirus entry tropism, we used reporter pseudovirions based on various cutaneous and mucosal papillomavirus species, including the recently identified murine papillomavirus. Pseudovirus transduction of BALB/c mice was examined using an improved murine skin infection protocol and a previously developed cervicovaginal challenge model. In the skin, HPV5, HPV6, HPV16, BPV1 and MusPV1 pseudovirions preferentially transduced keratinocytes at sites of trauma, similar to the genital tract. Skin infection, visualized by in vivo imaging using a luciferase reporter gene, peaked between days 2-3 and rapidly diminished for all pseudovirion types. Murine cutaneous and genital tissues were similarily permissive for pseudovirions of HPV types 5, 6, 8, 16, 18, 26, 44, 45, 51, 58 and animal papillomaviruses BPV1 and MusPV1, implying that papillomavirus' tissue and host tropism is governed primarily by post-entry regulatory events in the mouse.
authors.affiliation: Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Handisurya
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Day
authors.forename: Cynthia D
authors.initials: CD
authors.lastname: Thompson
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Buck
authors.forename: Kihyuck
authors.initials: K
authors.lastname: Kwak
authors.forename: Richard B S
authors.initials: RB
authors.lastname: Roden
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Lowy
authors.forename: John T
authors.initials: JT
authors.lastname: Schiller
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D017930:Genes, Reporter; D006801:Humans; D015603:Keratinocytes / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009092:Mucous Membrane / Q000821:virology; D009928:Organ Specificity; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity*; D030361:Papillomavirus Infections / Q000821:virology; D012867:Skin / Q000821:virology*; D013045:Species Specificity; D014621:Vagina / Q000382:microbiology*; D014771:Virion / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D014774:Virulence