title: Protective effect of diltiazem, a L-type calcium channel antagonist, on lysophosphatidylcholine-enhanced hydroxyl radical generation by MPP(+) in rat striatum.
abstract: BACKGROUND
  The interaction between the PKC and oxygen free radicals.
  
  
  OBJECTIVE
  The present study examined the effects of lysophosphatidylcholine (LPC), an endogenous amphophilic lipid metabolite, on 1-methyl-4-phenylpyridinium ion (MPP(+))-induced hydroxyl radical (()OH) generation in rat striatum. LPC (50 μM) increased ()OH formation by MPP(+). Further, the effect of diltiazem, a L-type Ca(2+) channel antagonist, on MPP(+) (5mM)-induced ()OH generation was studied.
  
  
  RESULTS
  Diltiazem (100 μM) significantly suppressed LPC and MPP(+)-induced ()OH generation. The results indicate ()OH generation, and diltiazem may have preventive effect on MPP(+)-induced ()OH generation.
  
  
  CONCLUSIONS
  These results demonstrated that diltiazem suppressed LPC-induced generation of free radical through blockage of Ca(2+) channel in the rat striatum.
authors.affiliation: Yokkaichi Nursing and Medical Care University, 1200 Kayo-cho, Yokkaichi-shi, Mie 512-8045, Japan. obata@y-nm.ac.jp
authors.forename: Toshio
authors.initials: T
authors.lastname: Obata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyashita
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D020746:Calcium Channels, L-Type / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism*; D004110:Diltiazem / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D017665:Hydroxyl Radical / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008297:Male; D020011:Protective Agents / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D008244:Lysophosphatidylcholines; D020011:Protective Agents; D017665:Hydroxyl Radical; D011493:Protein Kinase C; D004110:Diltiazem; D015655:1-Methyl-4-phenylpyridinium