title: Complete genome sequence of an astrovirus identified in a domestic rabbit (Oryctolagus cuniculus) with gastroenteritis.
abstract: A colony of domestic rabbits in Tennessee, USA, experienced a high-mortality (~90%) outbreak of enterocolitis. The clinical characteristics were one to six days of lethargy, bloating, and diarrhea, followed by death. Heavy intestinal coccidial load was a consistent finding as was mucoid enteropathy with cecal impaction. Preliminary analysis by electron microscopy revealed the presence of virus-like particles in the stool of one of the affected rabbits. Analysis using the Virochip, a viral detection microarray, suggested the presence of an astrovirus, and follow-up PCR and sequence determination revealed a previously uncharacterized member of that family. Metagenomic sequencing enabled the recovery of the complete viral genome, which contains the characteristic attributes of astrovirus genomes. Attempts to propagate the virus in tissue culture have yet to succeed. Although astroviruses cause gastroenteric disease in other mammals, the pathogenicity of this virus and the relationship to this outbreak remains to be determined. This study therefore defines a viral species and a potential rabbit pathogen.
authors.affiliation: Department of Medicine, University of California San Francisco, San Francisco, CA, USA.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Stenglein
authors.forename: Eric
authors.initials: E
authors.lastname: Velazquez
authors.forename: Cheryl
authors.initials: C
authors.lastname: Greenacre
authors.forename: Rebecca P
authors.initials: RP
authors.lastname: Wilkes
authors.forename: J Graham
authors.initials: JG
authors.lastname: Ruby
authors.forename: Julia S
authors.initials: JS
authors.lastname: Lankton
authors.forename: Donald
authors.initials: D
authors.lastname: Ganem
authors.forename: Melissa A
authors.initials: MA
authors.lastname: Kennedy
authors.forename: Joseph L
authors.initials: JL
authors.lastname: DeRisi
mesh_terms: D000818:Animals; D019200:Astroviridae / Q000235:genetics* / Q000302:isolation & purification; D019350:Astroviridae Infections / Q000662:veterinary / Q000821:virology; D004760:Enterocolitis / Q000662:veterinary / Q000821:virology; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000662:veterinary / Q000821:virology; D016679:Genome, Viral; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000235:genetics*; D011817:Rabbits; D017422:Sequence Analysis, DNA; D013714:Tennessee; D014776:Virus Cultivation
chemical_list: D012367:RNA, Viral