title: [Gene optimization, protein expression, purification and characterization of the HDV antigen for diagnosis].
abstract: OBJECTIVE
  To prepare HDAg with biological activities as a candidate of diagnostic reagent.
  
  
  METHODS
  To synthesize HDV gene fragment after codon optimization. To construct a thio-fused recombinant plasmid based on M48 expression vector. To express in E. coli induced by IPTG. To purify the protein by affinity chromatography followed by characterization in ELISA:
  
  
  RESULTS
  Plasmid construction was verified by enzyme digestion. SDS-PAGE indicated the molecular weight of the protein was the same as we expectation. ELISA proved its affinity with HDV antibodies.
  
  
  CONCLUSIONS
  HDAg was obtained successfully and it will pave the road to the research of HDV diagnostic reagent.
authors.affiliation: National Institute for Viral Disease Control and Prevention, Beijng 102206, China. jyd0311@126.com
authors.forename: Jun-Ying
authors.initials: JY
authors.lastname: Ding
authors.forename: Min-Zhuo
authors.initials: MZ
authors.lastname: Guo
authors.forename: Yao
authors.initials: Y
authors.lastname: Yi
authors.forename: Qiu-Dong
authors.initials: QD
authors.lastname: Su
authors.forename: Xin
authors.initials: X
authors.lastname: Lu Xue
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Bi
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D003699:Hepatitis D / Q000175:diagnosis*; D035923:Hepatitis delta Antigens / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification
chemical_list: D035923:Hepatitis delta Antigens; D011994:Recombinant Proteins