title: Role of neutrophil elastase in lung injury induced by burn-blast combined injury in rats.
abstract: OBJECTIVE
  Neutrophil elastase (NE) takes part in the pathogenesis of acute lung injury. However, its role in lung injury of burn-blast combined injury is unclear. Our objective was to assess the role of NE, and effect of sivelestat, a specific NE inhibitor, in lung injury induced by burn-blast combined injury in rats.
  
  
  METHODS
  One hundred and sixty male Sprague-Dawley rats were randomly subjected to burn-blast combined injury (BB) group, burn-blast combined injury plus sivelestat treatment (S) group or control (C) group. Blood gas, protein concentration and NE activity in bronchoalveolar lavage fluid (BALF), pulmonary myeloperoxidase (MPO) activity, serum concentrations of TNF-α and IL-8, etc. were investigated from 0 h to 7 d post-injury.
  
  
  RESULTS
  In BB group, PaO2 decreased, while NE activity in BALF, total protein concentration in BALF, pulmonary MPO activity and W/D ratio, serum concentrations of TNF-α and IL-8 increased with neutrophil infiltration, progressive bleeding and pulmonary oedema. Compared with BB group, sivelestat treatment decreased the NE activity and ameliorated the above indexes.
  
  
  CONCLUSIONS
  Sivelestat, exerts a protective effect in lung injury after burn-blast combined injury through inhibiting NE activity to decrease pulmonary vascular permeability, neutrophil sequestration, and production of TNF-α and IL-8.
authors.affiliation: Department of Burn and Plastic Surgery, Burns Institute, Burn & Plastic Hospital of PLA General Hospital, the First Affiliated Hospital of PLA General Hospital, Beijing 100048, PR China. cjk304@126.com
authors.forename: Jia-ke
authors.initials: JK
authors.lastname: Chai
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Cai
authors.forename: Hu-ping
authors.initials: HP
authors.lastname: Deng
authors.forename: Xiao-fang
authors.initials: XF
authors.lastname: Zou
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Qing-gang
authors.initials: QG
authors.lastname: Hu
authors.forename: Chuan-an
authors.initials: CA
authors.lastname: Shen
authors.forename: Hui-nan
authors.initials: HN
authors.lastname: Yin
authors.forename: Xi-bo
authors.initials: XB
authors.lastname: Zhang
authors.forename: Yun-fei
authors.initials: YF
authors.lastname: Chi
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Rui
authors.initials: R
authors.lastname: Feng
mesh_terms: D000818:Animals; D001753:Blast Injuries / Q000150:complications* / Q000188:drug therapy / Q000201:enzymology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002056:Burns / Q000150:complications* / Q000188:drug therapy / Q000201:enzymology; D002245:Carbon Dioxide / Q000378:metabolism; D004195:Disease Models, Animal; D005998:Glycine / Q000031:analogs & derivatives / Q000627:therapeutic use; D016209:Interleukin-8 / Q000378:metabolism; D019272:Leukocyte Elastase / Q000502:physiology*; D055370:Lung Injury / Q000188:drug therapy / Q000201:enzymology* / Q000209:etiology; D008297:Male; D010100:Oxygen / Q000378:metabolism; D010313:Partial Pressure; D053491:Proteinase Inhibitory Proteins, Secretory / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D015842:Serine Proteinase Inhibitors / Q000627:therapeutic use; D013449:Sulfonamides / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016209:Interleukin-8; D053491:Proteinase Inhibitory Proteins, Secretory; D015842:Serine Proteinase Inhibitors; D013449:Sulfonamides; D014409:Tumor Necrosis Factor-alpha; D002245:Carbon Dioxide; C069195:sivelestat; D019272:Leukocyte Elastase; D010100:Oxygen; D005998:Glycine