title: Measurement of inositol(1,4,5)trisphosphate using a stereospecific radioreceptor mass assay.
abstract: Inositol(1,4,5)trisphosphate [Ins(1,4,5)P(3)] is an important second messenger that activates its cognate Ins(1,4,5)P(3) receptor to release Ca(2+) from intracellular stores. The assay described in this chapter uses the Ins(1,4,5)P(3) receptor (essentially as a binding protein) to measure the biologically active trisphosphate (specifically from other trisphosphates). The binding protein (Ins(1,4,5)P3 receptor) is prepared from Bovine adrenal glands and this is mixed with [(3)H]-labeled and unlabeled (generated from biological samples or standards) Ins(1,4,5)P(3). Using the same principles as for radioimmunoassay/ELISA the mass of Ins(1,4,5)P(3) in biological samples can be estimated.
authors.affiliation: Neurology CEDD, GlaxoSmithKline Pharmaceuticals Ltd., Harlow, Essex, UK.
authors.forename: Darren
authors.initials: D
authors.lastname: Smart
mesh_terms: D000818:Animals; D002417:Cattle; D015544:Inositol 1,4,5-Trisphosphate / Q000737:chemistry / Q000378:metabolism*; D007295:Inositol Phosphates / Q000737:chemistry / Q000378:metabolism; D011869:Radioligand Assay / Q000379:methods*; D014316:Tritium / Q000737:chemistry / Q000378:metabolism
chemical_list: D007295:Inositol Phosphates; D014316:Tritium; D015544:Inositol 1,4,5-Trisphosphate