title: [Using of the luciferase reporter constructs for investigation of the capacity of Noggin2 protein to inhibit cell signaling pathways in early Xenopus laevis embryos].
abstract: Noggin (Noggin1) protein inhibits Smad1-dependent TGF-beta signaling pathway by extracellular binding of BMP proteins. Recently we found two previously unknown representatives of Noggin family - Noggin2 and Noggin4. Here we investigate by using the luciferase reporter constructs the ability of Noggin2 to inhibit BMP-, Activin/Nodal- and Wnt-signaling pathways in the early Xenopus laevis embryos. The effectiveness of this inhibitory activity of Noggin2 is comparing with that of well-known inhibitors of the indicated pathways - the secreted proteins Cerberus and Follistatin. Besides revealing of novel properties of Noggin2, our findings demonstrate that luciferase-reporter assay is as a useful tool for signaling pathways investigation in the model of Xenopus embryos.
authors.forename: F M
authors.initials: FM
authors.lastname: Eroshkin
authors.forename: A V
authors.initials: AV
authors.lastname: Baĭramov
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Martynova
authors.forename: A G
authors.initials: AG
authors.lastname: Zaraĭskiĭ
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D038681:Follistatin / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics; D015398:Signal Transduction / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000378:metabolism; D060449:Wnt Signaling Pathway; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism
chemical_list: D019485:Bone Morphogenetic Proteins; D038681:Follistatin; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; C576650:NOG2 protein, Xenopus; D016212:Transforming Growth Factor beta; D029867:Xenopus Proteins; C100866:cer1 protein, Xenopus; D008156:Luciferases