title: Isotopic labeling-assisted metabolomics using LC-MS.
abstract: Metabolomics has emerged as the latest of the so-called "omics" disciplines and has great potential to provide deeper understanding of fundamental biochemical processes at the biological system level. Among recent technological developments, LC-HRMS enables determination of hundreds to thousands of metabolites over a wide range of concentrations and has developed into one of the most powerful techniques in non-targeted metabolomics. The analysis of mixtures of in-vivo-stable isotopic-labeled samples or reference substances with un-labeled samples leads to specific LC-MS data patterns which can be systematically exploited in practically all data-processing steps. This includes recognition of true metabolite-derived analytical features in highly complex LC-MS data and characterization of the global biochemical composition of biological samples. In addition, stable-isotopic labeling can be used for more accurate quantification (via internal standardization) and identification of compounds in different organisms.
authors.affiliation: Center for Analytical Chemistry, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna, Konrad-Lorenz-Str 20, 3430 Tulln, Austria.
authors.forename: C
authors.initials: C
authors.lastname: Bueschl
authors.forename: R
authors.initials: R
authors.lastname: Krska
authors.forename: B
authors.initials: B
authors.lastname: Kluger
authors.forename: R
authors.initials: R
authors.lastname: Schuhmacher
mesh_terms: D002247:Carbon Isotopes / Q000737:chemistry; D002623:Chemistry Techniques, Analytical; D002853:Chromatography, Liquid / Q000379:methods*; D005670:Fusarium / Q000378:metabolism; D006801:Humans; D007553:Isotope Labeling; D007554:Isotopes / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D015233:Models, Statistical; D015203:Reproducibility of Results; D012984:Software
chemical_list: D002247:Carbon Isotopes; D007554:Isotopes