title: Use of 111-Indium-labeled autologous eosinophils to establish the in vivo kinetics of human eosinophils in healthy subjects.
abstract: Eosinophils are the major cellular effectors of allergic inflammation and represent an important therapeutic target. Although the genesis and activation of eosinophils have been extensively explored, little is known about their intravascular kinetics or physiological fate. This study was designed to determine the intravascular life span of eosinophils, their partitioning between circulating and marginated pools, and sites of disposal in healthy persons. Using autologous, minimally manipulated 111-Indium-labeled leukocytes with blood sampling, we measured the eosinophil intravascular residence time as 25.2 hours (compared with 10.3 hours for neutrophils) and demonstrated a substantial marginated eosinophil pool. γ camera imaging studies using purified eosinophils demonstrated initial retention in the lungs, with early redistribution to the liver and spleen, and evidence of recirculation from a hepatic pool. This work provides the first in vivo measurements of eosinophil kinetics in healthy volunteers and shows that 111-Indium-labeled eosinophils can be used to monitor the fate of eosinophils noninvasively.
authors.affiliation: Division of Respiratory Medicine, Department of Medicine, University of Cambridge School of Clinical Medicine, Cambridge, United Kingdom.
authors.forename: Neda
authors.initials: N
authors.lastname: Farahi
authors.forename: Nanak R
authors.initials: NR
authors.lastname: Singh
authors.forename: Sarah
authors.initials: S
authors.lastname: Heard
authors.forename: Chrystalla
authors.initials: C
authors.lastname: Loutsios
authors.forename: Charlotte
authors.initials: C
authors.lastname: Summers
authors.forename: Chandra K
authors.initials: CK
authors.lastname: Solanki
authors.forename: Kishor
authors.initials: K
authors.lastname: Solanki
authors.forename: Kottekkattu K
authors.initials: KK
authors.lastname: Balan
authors.forename: Prina
authors.initials: P
authors.lastname: Ruparelia
authors.forename: A Michael
authors.initials: AM
authors.lastname: Peters
authors.forename: Alison M
authors.initials: AM
authors.lastname: Condliffe
authors.forename: Edwin R
authors.initials: ER
authors.lastname: Chilvers
mesh_terms: D000328:Adult; D058948:Cell Tracking / Q000379:methods*; D004804:Eosinophils / Q000166:cytology* / Q000502:physiology; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D007205:Indium Radioisotopes / Q000008:administration & dosage* / Q000378:metabolism; D007700:Kinetics; D008297:Male; D013194:Staining and Labeling; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D007205:Indium Radioisotopes