title: Use of reprogrammed cells to identify therapy for respiratory papillomatosis.
abstract: A patient with a 20-year history of recurrent respiratory papillomatosis had progressive, bilateral tumor invasion of the lung parenchyma. We used conditional reprogramming to generate cell cultures from the patient's normal and tumorous lung tissue. Analysis revealed that the laryngeal tumor cells contained a wild-type 7.9-kb human papillomavirus virus type 11 (HPV-11) genome, whereas the pulmonary tumor cells contained a 10.4-kb genome. The increased size of the latter viral genome was due to duplication of the promoter and oncogene regions. Chemosensitivity testing identified vorinostat as a potential therapeutic agent. At 3 months after treatment initiation, tumor sizes had stabilized, with durable effects at 15 months.
authors.affiliation: Department of Pathology, Georgetown University Medical Center, Washington, DC 20057, USA.
authors.forename: Hang
authors.initials: H
authors.lastname: Yuan
authors.forename: Scott
authors.initials: S
authors.lastname: Myers
authors.forename: Jingang
authors.initials: J
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Woo
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Kallakury
authors.forename: Andrew
authors.initials: A
authors.lastname: Ju
authors.forename: Michael
authors.initials: M
authors.lastname: Bazylewicz
authors.forename: Yvonne M
authors.initials: YM
authors.lastname: Carter
authors.forename: Christopher
authors.initials: C
authors.lastname: Albanese
authors.forename: Nazaneen
authors.initials: N
authors.lastname: Grant
authors.forename: Aziza
authors.initials: A
authors.lastname: Shad
authors.forename: Anatoly
authors.initials: A
authors.lastname: Dritschilo
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Liu
authors.forename: Richard
authors.initials: R
authors.lastname: Schlegel
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002478:Cells, Cultured; D004279:DNA, Viral / Q000302:isolation & purification; D015870:Gene Expression; D016679:Genome, Viral; D052140:Human papillomavirus 11 / Q000235:genetics; D006801:Humans; D006877:Hydroxamic Acids / Q000627:therapeutic use*; D007822:Laryngeal Neoplasms / Q000601:surgery / Q000821:virology; D008168:Lung / Q000166:cytology*; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000821:virology; D008297:Male; D009154:Mutation; D030361:Papillomavirus Infections / Q000150:complications / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis; D012141:Respiratory Tract Infections / Q000150:complications / Q000188:drug therapy* / Q000473:pathology / Q000601:surgery; D014407:Tumor Cells, Cultured; D000077337:Vorinostat; D055815:Young Adult
chemical_list: D000970:Antineoplastic Agents; D004279:DNA, Viral; D006877:Hydroxamic Acids; D012333:RNA, Messenger; D012367:RNA, Viral; D000077337:Vorinostat