title: High level lipid production by a novel inulinase-producing yeast Pichia guilliermondii Pcla22.
abstract: In this study, an inulinase-producing yeast strain Pcla22 of Pichia guilliermondii was identified. It was found that the yeast strain Pcla22 could produce higher amount of oil and more lipid bodies in its cells than any other yeast strains tested in this study. Under the optimal conditions, 60.6%(w/w) of lipid based on cell dry weight, 20.4 g/l of the dry cell mass, SCO produced per g of consumed sugar of 0.19 g/g and biomass produced per g of consumed sugar of 0.32 g/g were obtained in the culture of the yeast strain Pcla22 after 96 h of the fed-batch fermentation. Over 79.8% of the fatty acids from the yeast strain Pcla22 grown in the oil production medium containing inulin was C(16:0) and C(18:1), especially C(18:1) (57.9%). The biodiesel obtained from the produced lipid could be burnt well.
authors.affiliation: Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Qingdao, China.
authors.forename: Guang-Yuan
authors.initials: GY
authors.lastname: Wang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chi
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Wang
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Chi
mesh_terms: D056804:Biofuels; D018533:Biomass; D002455:Cell Division; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D007444:Inulin / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D010843:Pichia / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction
chemical_list: D056804:Biofuels; D008055:Lipids; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase; D009584:Nitrogen