title: In vitro estrogenicity of dental resin sealants.
abstract: OBJECTIVE
  This investigation assessed the estrogenic action of various types of sealants.
  
  
  METHODS
  Three light-cured sealants (Denton Clear, Delton Opaque, Ultraseal XT Plus) were applied in polyethylene molds (Ø:10mm, h:2mm, n:8) and photopolymerized (40 seconds, halogen bulb unit, standard mode 650 mW/cm 2 intensity). All specimens were immersed in normal saline for 1 week at 37 °C. Samples of eluents at concentrations of 5% and 10% (volume per volume) were tested for estrogenicity by measuring their effect on the proliferation of the estrogen responsive MCF-7 breast cancer cells. In addition, an estrogen insensitive cell line was used as a control (MDA-MB-231) to exclude a possible cytostatic effect of the tested materials. All assays were repeated 4 times, and results analyzed with a 2-way analysis of variance and Tukey's test at the 0.05 level.
  
  
  RESULTS
  Eluents of the sealants tested at concentrations of 5% and 10% did not possess estrogenicity, except for the eluent of one sealant (Delton Opaque) at concentration 10%, which caused an induction of the proliferation rate of the MCF-7 cells.
  
  
  CONCLUSIONS
  Eluents of sealants tested at a concentration of 5% had no estrogenic activity. The eluent of Delton Opaque at a concentration of 10% possessed some estrogenic activity.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, University of Athens, Athens, Greece.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Zampeli
authors.forename: Liza
authors.initials: L
authors.lastname: Papagiannoulis
authors.forename: George
authors.initials: G
authors.lastname: Eliades
authors.forename: Harris
authors.initials: H
authors.lastname: Pratsinis
authors.forename: Dimitris
authors.initials: D
authors.lastname: Kletsas
authors.forename: Theodore
authors.initials: T
authors.lastname: Eliades
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004967:Estrogens / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D010895:Pit and Fissure Sealants; D012117:Resins, Synthetic
chemical_list: D004967:Estrogens; D010895:Pit and Fissure Sealants; D012117:Resins, Synthetic