title: Complete genome sequence of a novel Newcastle disease virus strain isolated from a chicken in West Africa.
abstract: The complete genome sequence of an African Newcastle disease virus (NDV) strain isolated from a chicken in Togo in 2009 was determined. The genome is 15,198 nucleotides (nt) in length and is classified in genotype VII in the class II cluster. Compared to common vaccine strains, the African strain contains a previously described 6-nt insert in the downstream untranslated region of the N gene and a novel 6-nt insert in the HN-L intergenic region. Genome length differences are a marker of the natural history of NDV. This is the first description of a class II NDV strain with a genome of 15,198 nt and a 6-nt insert in the HN-L intergenic region. Sequence divergence relative to vaccine strains was substantial, likely contributes to outbreaks, and illustrates the continued evolution of new NDV strains in West Africa.
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland, USA.
authors.forename: Shin-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Subhashree
authors.initials: S
authors.lastname: Nayak
authors.forename: Anandan
authors.initials: A
authors.lastname: Paldurai
authors.forename: Baibaswata
authors.initials: B
authors.lastname: Nayak
authors.forename: Arthur
authors.initials: A
authors.lastname: Samuel
authors.forename: Gilbert L
authors.initials: GL
authors.lastname: Aplogan
authors.forename: Kodzo A
authors.initials: KA
authors.lastname: Awoume
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Webby
authors.forename: Mariette F
authors.initials: MF
authors.lastname: Ducatez
authors.forename: Peter L
authors.initials: PL
authors.lastname: Collins
authors.forename: Siba K
authors.initials: SK
authors.lastname: Samal
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014037:Togo