title: Antiadhesive and antibacterial multilayer films via layer-by-layer assembly of TMC/heparin complexes.
abstract: N-Trimethyl chitosan (TMC), an antibacterial agent, and heparin (HP), an antiadhesive biopolymer, were alternately deposited on modified polystyrene films, as substrates, to built antiadhesive and antibacterial multilayer films. The properties of the multilayer films were investigated by Fourier transform infrared spectroscopy, atomic force microscopy, scanning electron microscopy, and Kelvin force microscopy. In vitro studies of controlled release of HP were evaluated in simulated intestinal fluid and simulated gastric fluid. The initial adhesion test of  E. coli  on multilayer films surface showed effective antiadhesive properties. The in vitro antibacterial test indicated that the multilayer films of TMC/HP based on TMC80 can kill the  E. coli  bacteria. Therefore, antiadhesive and antibacterial multilayer films may have good potential for coatings and surface modification of biomedical applications.
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC, Departamento de Química, DBS Bloco B-08, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Heveline D M
authors.initials: HD
authors.lastname: Follmann
authors.forename: Alessandro F
authors.initials: AF
authors.lastname: Martins
authors.forename: Adriana P
authors.initials: AP
authors.lastname: Gerola
authors.forename: Thiago A L
authors.initials: TA
authors.lastname: Burgo
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.forename: Adley F
authors.initials: AF
authors.lastname: Rubira
authors.forename: Edvani C
authors.initials: EC
authors.lastname: Muniz
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects*; D001672:Biocompatible Materials / Q000737:chemistry*; D001704:Biopolymers; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D006493:Heparin / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011137:Polystyrenes; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D001704:Biopolymers; C118071:N-trimethyl chitosan chloride; D011137:Polystyrenes; D006493:Heparin; D048271:Chitosan