title: Isolation of total RNA and detection procedures for miRNA present in bovine-cultured adipocytes and adipose tissues.
abstract: Micro-ribonucleic acids (miRNA) regulate gene expression posttranscriptionally by altering translation of protein(s) encoded by specific messenger RNA. Therefore the ability to detect and quantify the expression levels of specific miRNA present within a cell or tissue is necessary to thoroughly examine cellular physiology and gene expression. Here we describe procedures that allow for the isolation and quantification of miRNA in bovine adipocytes and adipose tissue.
authors.affiliation: Animal and Veterinary Science Department, Clemson University, Clemson, SC, USA. scottp@clemson.edu
authors.forename: Scott L
authors.initials: SL
authors.lastname: Pratt
authors.forename: T Ashley
authors.initials: TA
authors.lastname: Burns
authors.forename: Marcy D
authors.initials: MD
authors.lastname: Owens
authors.forename: Susan K
authors.initials: SK
authors.lastname: Duckett
mesh_terms: D017667:Adipocytes / Q000737:chemistry* / Q000378:metabolism; D000273:Adipose Tissue / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D005786:Gene Expression Regulation; D035683:MicroRNAs / Q000032:analysis*; D012313:RNA / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods
chemical_list: D035683:MicroRNAs; D012313:RNA