title: Unprecedented hydroxyl radical-dependent two-step chemiluminescence production by polyhalogenated quinoid carcinogens and H2O2.
abstract: Most chemiluminescence (CL) reactions usually generate only one-step CL, which is rarely dependent on the highly reactive and biologically/environmentally important hydroxyl radicals ((•)OH). Here, we show that an unprecedented two-step CL can be produced by the carcinogenic tetrachloro-1,4-benzoquinone (also known as p-chloranil) and H(2)O(2), which was found to be well-correlated to and directly dependent on its two-step metal-independent production of (•)OH. We proposed that (•)OH-dependent formation of quinone-dioxetane and electronically excited carbonyl species might be responsible for this unusual two-step CL production by tetrachloro-1,4-benzoquinone/H(2)O(2). This is a unique report of a previously undefined two-step CL-producing system that is dependent on intrinsically formed (•)OH. These findings may have potential applications in detecting and quantifying (•)OH and the ubiquitous polyhalogenated aromatic carcinogens, which may have broad biological and environmental implications for future research on these types of important species.
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People's Republic of China. bzhu@rcees.ac.cn
authors.forename: Ben-Zhan
authors.initials: BZ
authors.lastname: Zhu
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Huang
authors.forename: Hao
authors.initials: H
authors.lastname: Qin
authors.forename: Rui-Mei
authors.initials: RM
authors.lastname: Fan
authors.forename: Balaraman
authors.initials: B
authors.lastname: Kalyanaraman
authors.forename: Jun-Ge
authors.initials: JG
authors.lastname: Zhu
mesh_terms: D002273:Carcinogens / Q000737:chemistry*; D002703:Chloranil / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005453:Fluorescence; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D049449:Luminescence; D008956:Models, Chemical; D015394:Molecular Structure
chemical_list: D002273:Carcinogens; D002703:Chloranil; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide