title: Investigation of molecular and protein crystals by three photon polarization resolved microscopy.
abstract: We implement three photon fluorescence polarization resolved microscopy to optically investigate molecular and protein crystals. The availability of UV transitions using IR pulses allows analyses without fluorescence staining. Polarization resolved studies indicate that high-order symmetry structures can be revealed and that strong fluorescent energy transfer occurs between molecules. We show how this permits identification of a monocrystalline nature for a sample at the subwavelength resolution scale.
authors.affiliation: Institut Fresnel, CNRS, Aix-Marseille Université, Ecole Centrale Marseille, Campus de St Jérôme, 13013 Marseille, France.
authors.forename: Alicja
authors.initials: A
authors.lastname: Gasecka
authors.forename: Patrick
authors.initials: P
authors.lastname: Tauc
authors.forename: Anita
authors.initials: A
authors.lastname: Lewit-Bentley
authors.forename: Sophie
authors.initials: S
authors.lastname: Brasselet
mesh_terms: D000818:Animals; D003460:Crystallization; D004527:Egg Proteins / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008859:Microscopy, Polarization / Q000379:methods; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
chemical_list: D004527:Egg Proteins; D011506:Proteins; D009113:Muramidase