title: Evaluation of the dry sheet medium (Compact Dry ETB(R)) for enumerating Enterobacteriaceae in meat samples.
abstract: Compact Dry ETB(R)  (CD-ETB, ETB; Enterobacteriaceae) , a ready-to-use and self-diffusible dry medium sheet culture system for the detection and enumeration of Enterobacteriaceae, was evaluated. A total of 35 Enterobacteriaceae strains, which were studied for the inclusivity study, grew and formed red-purple colored colonies on the CD-ETB(R). When 17 gram-negative bacteria other than Enterobacteriaceae were inoculated for the exclusivity study, 2 strains grew and formed red-purple colonies, 9 strains formed colorless colonies, and 6 strains failed to grow. A total of 43 gram-positive bacteria and 3 yeasts failed to grow. The CD-ETB method was compared with the Violet Red Bile Glucose  (VRBG)  agar method according to ISO 21528-2 and the 3M Petrifilm(TM) EB  (3M-EB(R))  method in 53 meat samples. The correlation coefficients between CD-ETB(R) and VRBG agar, CD-ETB(R) and 3M-EB(R), and 3M-EB(R) and VRBG agar were 0.962, 0.992 and 0.960, respectively.
authors.affiliation: Nissui Pharmaceutical Co., Ltd. 1075-2 Hokunanmoro, Yuki, Ibaraki 307-0036, Japan. h-teramura@yki.nissui-pharm.jp
authors.forename: Hajime
authors.initials: H
authors.lastname: Teramura
authors.forename: Shingo
authors.initials: S
authors.lastname: Mizuochi
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Kodaka
mesh_terms: D015169:Colony Count, Microbial / Q000295:instrumentation / Q000379:methods*; D003470:Culture Media / Q000378:metabolism; D004755:Enterobacteriaceae / Q000254:growth & development* / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000382:microbiology*
chemical_list: D003470:Culture Media