title: Dynamic exchange of myosin VI on endocytic structures.
abstract: The actin-based molecular motor myosin VI functions in the endocytic uptake pathway, both during the early stages of clathrin-mediated uptake and in later transport to/from early endosomes. This study uses fluorescence recovery after photobleaching (FRAP) to examine the turnover rate of myosin VI during endocytosis. The results demonstrate that myosin VI turns over dynamically on endocytic structures with a characteristic half-life common to both the large insert isoform of myosin VI on clathrin-coated structures and the no-insert isoform on early endosomes. This half-life is shared by the myosin VI-binding partner Dab2 and is identical for full-length myosin VI and the cargo-binding tail region. The 4-fold slower half-life of an artificially dimerized construct of myosin VI on clathrin-coated structures suggests that wild type myosin VI does not function as a stable dimer, but either as a monomer or in a monomer/dimer equilibrium. Taken together, these FRAP results offer insight into both the basic turnover dynamics and the monomer/dimer nature of myosin VI.
authors.affiliation: Laboratory of Molecular Physiology, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Bond
authors.forename: Susan D
authors.initials: SD
authors.lastname: Arden
authors.forename: John
authors.initials: J
authors.lastname: Kendrick-Jones
authors.forename: Folma
authors.initials: F
authors.lastname: Buss
authors.forename: James R
authors.initials: JR
authors.lastname: Sellers
mesh_terms: D000818:Animals; D016466:CHO Cells; D002966:Clathrin / Q000737:chemistry; D006224:Cricetinae; D019281:Dimerization; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008954:Models, Biological; D018995:Myosin Heavy Chains / Q000378:metabolism*; D009218:Myosins / Q000737:chemistry; D038761:Photobleaching; D020033:Protein Isoforms; D020798:Two-Hybrid System Techniques
chemical_list: D002966:Clathrin; D020033:Protein Isoforms; C089785:myosin VI; D049452:Green Fluorescent Proteins; D018995:Myosin Heavy Chains; D009218:Myosins