title: Phosphorylcholine-containing lipid molecular species profiling in biological tissue using a fast HPLC/QqQ-MS method.
abstract: A fast reversed-phase liquid chromatography-electrospray ionization triple quadrupole mass spectrometry method was developed for the molecular species profiling of glycerophosphocholine (GPC) and sphingomyelin (SM) in total lipid extracts. A two-stage mass spectrometry strategy was adopted to analyze in detail the composition of lipid molecular species. Precursor ion analysis was first conducted to obtain the preliminary composition profile of the phosphorylcholine-containing lipid. The product ion spectra were sequentially acquired for each recorded signal to determine the molecular structure of the lipid. A total of 150 GPCs and 12 SMs were identified in the fetal mouse lung with relative amounts ranging from 13.7% to less than 0.002% (normalizing by the total signal response). A column packed with core-shell particles was used to obtain excellent chromatographic separation with a shorter time demand in a conventional high-performance liquid chromatography system. Considering the compromise between the chromatographic efficiency and the electrospray signal response, the optimization of the mobile phase improves the chromatographic plate number to approximately 40,000 and the detection limits to less than 0.001 mg/L. The applicability of the method was validated through a study of chemically induced early lung maturation. The metabolic alteration in the fetal mouse lung was clearly reflected in the GPC and SM composition with several characteristics of the molecular structure that related to the character of the phospholipid layer upon the epithelial lining of alveoli and the relevant cell function. The results indicated that this analytical strategy is reliable for comprehensive molecular species profiling of GPC and SM and might be extended to the analysis of other phospholipids.
authors.affiliation: National Museum of Marine Biology and Aquarium, 2 Houwan Rd., Checheng, Pingtung 944, Taiwan. chtang@nmmba.gov.tw
authors.forename: Chuan-Ho
authors.initials: CH
authors.lastname: Tang
authors.forename: Po-Nien
authors.initials: PN
authors.lastname: Tsao
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Lin
authors.forename: Lee-Shing
authors.initials: LS
authors.lastname: Fang
authors.forename: Shu-Hui
authors.initials: SH
authors.lastname: Lee
authors.forename: Wei-Hsien
authors.initials: WH
authors.lastname: Wang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D057230:Limit of Detection; D008055:Lipids / Q000032:analysis; D008168:Lung / Q000737:chemistry* / Q000196:embryology*; D051379:Mice; D010767:Phosphorylcholine / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013109:Sphingomyelins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D008055:Lipids; D013109:Sphingomyelins; D010767:Phosphorylcholine