title: Induction of ARI12 upon broad band UV-B radiation is suppressed by UVR8 and cryptochromes.
abstract: ARI12 belongs to a family of 16 potential E3 ligases in Arabidopsis and is strongly induced in leaves upon low and high fluence rates (HFR) of UV-B. We have shown that ARI12 is a downstream target of the UV-B receptor, UVR8, and the transcription factors HY5 and HYH under low fluence rates. However under HFR of broad band UV-B ARI12 expression was still downstream of HY5 and HYH but increased in uvr8 mutants. To determine if other photoreceptors are responsible for the induction of ARI12 we quantified its expression in double mutants of the UV-A and blue light receptors, CRY1/2 and PHOT1/2, and the red light receptors PHYA/B. While the expression of ARI12 was increased in cyr1/2 it was unaffected in phot1/2 and phyA/B. Therefore ARI12 expression is suppressed by UVR8 and cryptochromes, and independent of phototropins and phytochromes A and B upon HFR of broad band UV-B.
authors.affiliation: Department of Applied Genetics und Cell Biology, BOKU-University of Natural Resources and Life Sciences, Vienna, Austria.
authors.forename: Lisi
authors.initials: L
authors.lastname: Xie
authors.forename: Marie-Theres
authors.initials: MT
authors.lastname: Hauser
mesh_terms: D017360:Arabidopsis / Q000378:metabolism* / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D056931:Cryptochromes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D010834:Phytochrome / Q000378:metabolism* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D029681:Arabidopsis Proteins; D056931:Cryptochromes; D010834:Phytochrome