title: Micropatterning of hydrogels on locally hydrophilized regions on PDMS by stepwise solution dipping and in situ gelation.
abstract: This study presents a simple but highly versatile method of fabricating picoliter-volume hydrogel patterns on poly(dimethylsiloxane) (PDMS) substrates. Hydrophilic regions were prepared on hydrophobic PDMS plates by trapping and melting functional polymer particles and performing subsequent reactions with partially oxidized dextran. Small aliquots of a gelation solution were selectively trapped on the hydrophilic areas by a simple dipping process that was utilized to make thin hydrogel patterns by the in situ gelation of a sol solution. Using this process, we successfully formed calcium alginate, collagen I, and chitosan hydrogels with a thickness of several micrometers and shapes that followed the hydrophilized regions. In addition, alginate and collagen gel patterns were used to capture cells with different adhesion properties selectively on or off the hydrogel structures. The presented strategy could be applicable to the preparation of a variety of hydrogels for the development of functional biosensors, bioreactors, and cell cultivation platforms.
authors.affiliation: Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, Inage-ku, Chiba, Japan.
authors.forename: Sari
authors.initials: S
authors.lastname: Sugaya
authors.forename: Shunta
authors.initials: S
authors.lastname: Kakegawa
authors.forename: Shizuka
authors.initials: S
authors.lastname: Fukushima
authors.forename: Masumi
authors.initials: M
authors.lastname: Yamada
authors.forename: Minoru
authors.initials: M
authors.lastname: Seki
mesh_terms: D000464:Alginates / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D012996:Solutions
chemical_list: D000464:Alginates; D024042:Collagen Type I; D004129:Dimethylpolysiloxanes; D006603:Hexuronic Acids; D020100:Hydrogels; D012996:Solutions; C013830:baysilon; D020723:Glucuronic Acid; D048271:Chitosan