title: Effects of dietary supplementation of inosine monophosphate on growth performance, innate immunity and disease resistance of olive flounder (Paralichthys olivaceus).
abstract: This study was investigated to examine the effects of dietary inosine monophosphate (IMP) supplementation on growth performance, feed utilization, innate immunity, hematological parameters and disease resistance of juvenile olive flounder. Five experimental diets were formulated to contain IMP at levels of 0, 0.1, 0.2, 0.4 and 1.0%. All diets were maintained isonitrogenous (48% crude protein) and isocaloric (20.7 MJ/kg diet). Triplicate groups of olive flounder (initial body weight, 7.5 ± 0.02 g) were fed one of the experimental diets to apparent satiation (twice a day) for 14 weeks. Final body weight of fish fed 0.1-0.2% IMP were significantly higher than that of fish fed the 1.0% IMP. Groups of fish fed 0.2 or 0.4% IMP diet had significantly higher myeloperoxidase and lysozyme activities than fish fed the control diet. However, nitro-blue-tetrazolium and superoxide dismutase activities were not significantly different among all treatments. In the challenge test against Streptococcus iniae, cumulative mortality of fish fed IMP supplemented diets was significantly lower (15%, 4%, 4% and 9% for 0.1%, 0.2%, 0.4% and 1.0% IMP, respectively) than that of fish fed the control group (87%). The results suggest that IMP supplementation of 0.46-1.84 g into a kg of fish meal based diet (0.1-0.4% IMP product) can enhance innate immunity and disease resistance of olive flounder.
authors.affiliation: Department of Marine Life Sciences, Jeju National University, 66 Jejudaehakro, Jeju 690-756, South Korea.
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Song
authors.forename: Se-Jin
authors.initials: SJ
authors.lastname: Lim
authors.forename: Kyeong-Jun
authors.initials: KJ
authors.lastname: Lee
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D017756:Aquaculture; D001774:Blood Chemical Analysis / Q000662:veterinary; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D060467:Disease Resistance; D004305:Dose-Response Relationship, Drug; D005393:Fish Diseases / Q000276:immunology* / Q000517:prevention & control; D005432:Flounder / Q000254:growth & development / Q000276:immunology*; D006400:Hematocrit / Q000662:veterinary; D007113:Immunity, Innate; D007291:Inosine Monophosphate / Q000008:administration & dosage*; D009113:Muramidase / Q000378:metabolism; D011897:Random Allocation; D016897:Respiratory Burst; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D013291:Streptococcus / Q000502:physiology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D007291:Inosine Monophosphate; D013482:Superoxide Dismutase; D009113:Muramidase