title: Rapid and direct detection of attomole adenosine triphosphate (ATP) by MALDI-MS using rutile titania chips.
abstract: We report the rutile titania-based capture of ATP and its application as a MALDI-MS target plate. This chip, when immersed in solutions containing different concentrations of ATP, can capture ATP and lead to its successful detection in MALDI-MS. We have optimized the ideal surface, showing an increased capture efficacy of the 900 °C (rutile) titania surfaces. We demonstrate the use of this chip as a target plate for direct analysis of the attached ATP using MALDI-MS, down to attomolar concentrations. This chip has a promising future for the detection of ATP in environmental samples, which may eventually be used as a pollution indicator in particular environments.
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan.
authors.forename: Muthu
authors.initials: M
authors.lastname: Manikandan
authors.forename: Nazim
authors.initials: N
authors.lastname: Hasan
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wu
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry; D000585:Aminacrine / Q000737:chemistry; D006358:Hot Temperature; D057230:Limit of Detection; D010084:Oxidation-Reduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
chemical_list: C009495:titanium dioxide; D000585:Aminacrine; D000255:Adenosine Triphosphate; D014025:Titanium