title: Structure-function analyses of plant type III polyketide synthases.
abstract: Plant type III polyketide synthases (PKSs) form a superfamily of biosynthetic enzymes involved in the production of a plethora of polyketide-derived natural products important for ecological adaptations and the fitness of land plants. Moreover, tremendous interest in bioengineering of type III PKSs to produce high-value compounds is increasing. Compared to type I and type II PKSs, which form either large modular protein complexes or dissociable molecular assemblies, type III PKSs exist as smaller homodimeric proteins, technically more amenable for detailed quantitative biochemical and phylogenetic analyses. In this chapter, we summarize a collection of approaches, including bioinformatics, genetics, protein crystallography, in vitro biochemistry, and mutagenesis, together affording a comprehensive interrogation of the structure-function-evolutionary relationships in the plant type III PKS family.
authors.affiliation: Howard Hughes Medical Institute, Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, California, USA.
authors.forename: Jing-Ke
authors.initials: JK
authors.lastname: Weng
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Noel
mesh_terms: D000217:Acyltransferases / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics; D020134:Catalytic Domain; D003066:Coenzyme A Ligases / Q000737:chemistry / Q000235:genetics; D019295:Computational Biology / Q000379:methods; D003653:Decarboxylation; D004789:Enzyme Activation; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D060847:Metabolic Engineering / Q000379:methods; D016297:Mutagenesis, Site-Directed; D010802:Phylogeny; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D029681:Arabidopsis Proteins; D011993:Recombinant Fusion Proteins; D000217:Acyltransferases; C491135:AT5G13930 protein, Arabidopsis; C020141:flavanone synthetase; C511052:4CL1 protein, Arabidopsis; D003066:Coenzyme A Ligases