title: Nitric oxide enhances development of lateral roots in tomato (Solanum lycopersicum L.) under elevated carbon dioxide.
abstract: Elevated carbon dioxide (CO₂) has been shown to enhance the growth and development of plants, especially of roots. Amongst them, lateral roots play an important role in nutrient uptake, and thus alleviate the nutrient limitation to plant growth under elevated CO₂. This paper examined the mechanism underlying CO₂ elevation-induced lateral root formation in tomato. The endogenous nitric oxide (NO) in roots was detected by the specific probe 4-amino-5-methylamino-2',7'-difluorofluorescein diacetate (DAF-FM DA). We suggest that CO₂ elevation-induced NO accumulation was important for lateral root formation. Elevated CO₂ significantly increased the activity of nitric oxide synthase in roots, but not nitrate reductase activity. Moreover, the pharmacological evidence showed that nitric oxide synthase rather than nitrate reductase was responsible for CO₂ elevation-induced NO accumulation. Elevated CO₂ enhanced the activity of nitric oxide synthase and promoted production of NO, which was involved in lateral root formation in tomato under elevated CO₂.
authors.affiliation: Ministry of Education Key Laboratory of Environment Remediation and Ecosystem Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.forename: Wendan
authors.initials: W
authors.lastname: Xiao
authors.forename: Yaofang
authors.initials: Y
authors.lastname: Niu
authors.forename: Chongwei
authors.initials: C
authors.lastname: Jin
authors.forename: Rushan
authors.initials: R
authors.lastname: Chai
authors.forename: Caixian
authors.initials: C
authors.lastname: Tang
authors.forename: Yongsong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism* / Q000494:pharmacology; D005452:Fluoresceins / Q000378:metabolism; D018551:Solanum lycopersicum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018613:Microscopy, Confocal; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D050901:Nitrate Reductase / Q000037:antagonists & inhibitors / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000378:metabolism / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D009599:Nitroprusside / Q000378:metabolism / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D017973:Tungsten Compounds / Q000494:pharmacology
chemical_list: C503301:4-amino-5-methylamino-2',7'-difluorofluorescein diacetate; D005452:Fluoresceins; D020030:Nitric Oxide Donors; D017973:Tungsten Compounds; D002245:Carbon Dioxide; D009599:Nitroprusside; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D050901:Nitrate Reductase; C045951:tungstate; D019331:NG-Nitroarginine Methyl Ester