title: Problem-solving test: signal transduction in Philadelphia chromosome positive leukemia cells.
abstract: Terms to be familiar with before you start to solve the test: reciprocal translocation, proto-oncogene, gene expression, c-Abl, tyrosine protein kinases, transmembrane protein, G protein, Src protein, malignant transformation, transfection, immunoprecipitation, pre-immune serum, [γ-(32)P]ATP, cAMP, SDS-polyacrylamide gel electrophoresis, autoradiography, Western blot (immunoblot), co-immunoprecipitation, expression vector, cDNA, transient transfection, reporter gene, reporter plasmid, promoter, Ras protein, transformed foci, extracellular signal-regulated kinase.
authors.affiliation: Department of Medical Biology, Medical School, University of Pécs, H-7624 Pécs, Hungary. jozsef.szeberenyi@aok.pte.hu
authors.forename: József
authors.initials: J
authors.lastname: Szeberényi
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D016044:Fusion Proteins, bcr-abl / Q000502:physiology; D051380:GRB2 Adaptor Protein / Q000502:physiology; D006801:Humans; D047468:Immunoprecipitation; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000378:metabolism; D010677:Philadelphia Chromosome; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism; D018794:Problem-Based Learning / Q000379:methods*; D000090063:Proto-Oncogene Mas; D015398:Signal Transduction
chemical_list: D051380:GRB2 Adaptor Protein; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016044:Fusion Proteins, bcr-abl