title: [Preventive effect of exogenous hydrogen sulfide on hepatic fibrosis in rats].
abstract: OBJECTIVE
  To determine the effect of exogenous hydrogen sulfide on experimental hepatic fibrosis in rats and its mechanism.
  
  
  METHODS
  Wistar male rats were randomly divided into three groups: a normal control group (n=8), a model group (n=8), and a hydrogen sulfide prevention group (n=8). The rat model of hepatic fibrosis was reduced by intraperitoneal injection of carbon tetrachloride (CCl4). The prevention group, in addition to intraperitoneal injection of 40% CCl4, was intraperitoneally administered H2S once a day until 8th week. After the experiment, the liver function and liver fibrosis were assayed. Liver tissue samples were used for histopathological changes. The expression of TGF-β1 in liver tissue was detected by RT-PCR and Western blot.
  
  
  RESULTS
  Compared with the model group, the levels of ALT, AST, HA, LN, and PC III in the sulfide group were significantly reduced (P<0.01 or P<0.05), ALB content was increased (P<0.05) in the serum, TGF-β1 expression was obviously reduced, and the degree of liver fibrosis was improved (P<0.05).
  
  
  CONCLUSIONS
  Exogenous hydrogen sulfide can effectively inhibit the development of hepatic fibrosis, reduce the expression of TGF-β1, and decrease the the sediment of extracellular matrix in the liver tissues.
authors.affiliation: Department of Clinical Medicine, Shandong Medical College, Jinan, China. sqhsdy@163.com
authors.forename: Qinhai
authors.initials: Q
authors.lastname: Shen
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Qin
authors.forename: Aimei
authors.initials: A
authors.lastname: Lu
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride; D005109:Extracellular Matrix / Q000378:metabolism*; D006862:Hydrogen Sulfide / Q000494:pharmacology* / Q000627:therapeutic use; D008099:Liver / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D002251:Carbon Tetrachloride; D006862:Hydrogen Sulfide