title: [Analysis of COX1 sequences of Taenia isolates from four areas of Guangxi].
abstract: OBJECTIVE
  To analyze the COX1 sequences of Taenia isolates from four areas of Guangxi Zhuang Autonomous Region, and to understand the distribution of Taenia asiatica in Guangxi.
  
  
  METHODS
  Patients with taeniasis in Luzhai, Rongshui, Tiandong and Sanjiang in Guangxi were treated by deworming, and the Taenia isolates were collected. Cyclooxygenase-1 (COX1) sequences of these isolates were amplified by PCR, and the PCR products were sequenced by T-A clone sequencing. The homogeneities and genetic distances were calculated and analyzed, and the phylogenic trees were constructed by some softwares. Meanwhile, the COX1 sequences of the isolates from the 4 areas were compared separately with the sequences of Taenia species in GenBank.
  
  
  RESULTS
  The COX1 sequence of the 5 Taenia isolates collected had the same length of 444 bp. There were 5 variable positions between the Luzhai isolate and Taenia asiatica, the homogeneity was 98.87% and their genetic distance was 0.011. The phylogenetic tree analysis revealed that the Luzhai isolate and Taenia asiatica locating at the same node had a close relationship. The homogeneity between Rongshui isolate A and Taenia solium was 100%, while the homogeneity of Rongshui isolate B with Taeniasis saginata and Taenia asiatica were 98.20% and 96.17%, respectively. The homogeneities of the Tiandong and Sanjiang isolates with Taenia solium were 99.55% and 96.40%, respectively, and the genetic distances were 0.005 and 0.037, respectively. The homogeneity between the Luzhai isolate and Taeniasis saginate was 96.40%.
  
  
  CONCLUSIONS
  Taenia asiatica exists in Luzhai and Taenia solium and Taenia saginata coexist in Rongshui, Guangxi Zhuang Autonomous Region.
authors.affiliation: Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 530021, China.
authors.forename: Yi-Chao
authors.initials: YC
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Ou-Yang
authors.forename: Ai-Rong
authors.initials: AR
authors.lastname: Su
authors.forename: Xiao-Ling
authors.initials: XL
authors.lastname: Wan
authors.forename: Shu-Lin
authors.initials: SL
authors.lastname: Li
mesh_terms: D000818:Animals; D051545:Cyclooxygenase 1 / Q000235:genetics*; D006801:Humans; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013621:Taenia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D051545:Cyclooxygenase 1