title: Selection and optimisation of a method for efficient metabolites extraction from microalgae.
abstract: Over the last decade, the use of microalgae for biofuel production and carbon dioxide sequestration has become a challenge worldwide. Processing costs are still too high for these methods to be profitable though, leading to a need to find high value by-products to optimise the added value of this biomass. For high-throughput screening of such metabolites, it is essential to reach the inner content of the cell. This paper presents research and development of a technique enabling a high extraction yield of any metabolite, taking into account the difficulty of extracting bound and or inaccessible molecules with a wide variety of polarities. To this end, several disruption techniques were tested at laboratory scale on two biological models: Porphyridium purpureum and Phaeodactylum tricornutum. A mixer mill gave the best results, offering access to a broad diversity of metabolites from microalgae for high-throughput screening.
authors.affiliation: IFREMER, Laboratoire de Physiologie et Biotechnologie des Algues, 44311 Nantes, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Serive
authors.forename: Raymond
authors.initials: R
authors.lastname: Kaas
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Bérard
authors.forename: Virginie
authors.initials: V
authors.lastname: Pasquet
authors.forename: Laurent
authors.initials: L
authors.lastname: Picot
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Cadoret
mesh_terms: D056804:Biofuels; D002245:Carbon Dioxide / Q000302:isolation & purification; D058086:Microalgae / Q000378:metabolism*
chemical_list: D056804:Biofuels; D002245:Carbon Dioxide