title: New genetic findings in parotid gland pleomorphic adenomas.
abstract: BACKGROUND
  Despite numerous studies, the tumor biology of pleomorphic adenomas, the most common salivary gland tumors, is still not completely defined. In order to identify further candidate genes important for tumor biology of pleomorphic adenomas, extended cytogenetic and molecular analysis are mandatory.
  
  
  METHODS
  We performed a detailed molecular cytogenetic analysis using comparative genomic hybridization (CGH) followed by fluorescence in situ hybridization (FISH) with probes for chromosome X, 16p, 17, and 20 on a large cohort of pleomorphic adenomas (n = 29).
  
  
  RESULTS
  We could confirm previously described deletions in pleomorphic adenomas affecting 16p, 17, 20q, and 22 by FISH and/or CGH analysis. Moreover, our CGH study revealed novel candidate regions on 8p23.1pter, 9p, 10q25.1q25.3, and 11q24qter in the series of analyzed pleomorphic adenomas.
  
  
  CONCLUSIONS
  Our present study reveals new insights in novel candidate regions implicated in pleomorphic adenoma tumorigenesis which should be considered in further molecular studies.
authors.affiliation: Department of Otorhinolaryngology, Saarland University Medical Center, Homburg/Saar, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Wemmert
authors.forename: Vivienne
authors.initials: V
authors.lastname: Willnecker
authors.forename: Christian
authors.initials: C
authors.lastname: Brunner
authors.forename: Gentiana Ioana
authors.initials: GI
authors.lastname: Wenzel
authors.forename: Birgit
authors.initials: B
authors.lastname: Sauter
authors.forename: Heike
authors.initials: H
authors.lastname: Meinelt
authors.forename: Nadia
authors.initials: N
authors.lastname: Bartholmé
authors.forename: Carolin
authors.initials: C
authors.lastname: Saada
authors.forename: Rainer Maria
authors.initials: RM
authors.lastname: Bohle
authors.forename: Steffi
authors.initials: S
authors.lastname: Urbschat
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schick
mesh_terms: D008949:Adenoma, Pleomorphic / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D000328:Adult; D000368:Aged; D063646:Carcinogenesis / Q000235:genetics*; D002869:Chromosome Aberrations; D015331:Cohort Studies; D020732:Cytogenetic Analysis / Q000379:methods; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease / Q000453:epidemiology*; D023281:Genomics; D005858:Germany; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010307:Parotid Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D011300:Preoperative Care / Q000379:methods; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D012680:Sensitivity and Specificity