title: Plasma myeloperoxidase levels correlate with hepatocellular carcinoma in chronic hepatitis C.
abstract: Myeloperoxidase (MPO) is an enzyme responsible for generating hypochlorous acid and reactive oxidants that may lead to liver injury and cancer in hepatitis C (HCV) infection. MPO expression level is regulated by a polymorphism in the promoter region -463 of MPO gene. In the current study, MPO plasma levels and the G-463A MPO polymorphism were determined in 158 chronically HCV infected patients with and without hepatocellular carcinoma (HCC). MPO plasma levels were determined using a commercially ELISA kit. The G-463A MPO polymorphism was accessed by real time PCR using TaqMan probes. The MPO plasma levels of patients with HCV-HCC were higher in comparison to patients with chronic hepatitis or with those patients with severe fibrosis (p=0.01 and p=0.04, respectively). The MPO G-463A polymorphism was not associated with HCV outcome. These findings suggest MPO levels monitoring may be a potential biological marker to HCC screening in patients with HCV.
authors.affiliation: Institute of Biological Science, University of Pernambuco, Recife, Pernambuco, Brazil. carmorodrigof@gmail.com
authors.forename: Rodrigo Feliciano
authors.initials: RF
authors.lastname: do Carmo
authors.forename: Débora Bezerra
authors.initials: DB
authors.lastname: de Almeida
authors.forename: Dayse Célia Barbosa Lins
authors.initials: DC
authors.lastname: Aroucha
authors.forename: Luydson Richardson Silva
authors.initials: LR
authors.lastname: Vasconcelos
authors.forename: Adriano Claudio Pereira
authors.initials: AC
authors.lastname: de Moraes
authors.forename: Maria do Socorro
authors.initials: Mdo S
authors.lastname: de Mendonça Cavalcanti
authors.forename: Clarice Neuenschwander Lins
authors.initials: CN
authors.lastname: de Morais
authors.forename: Leila Maria Moreira Beltrão
authors.initials: LM
authors.lastname: Pereira
authors.forename: Patrícia
authors.initials: P
authors.lastname: Moura
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000150:complications / Q000235:genetics; D003062:Codon; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D019698:Hepatitis C, Chronic / Q000097:blood* / Q000150:complications / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000150:complications / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000097:blood* / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D055815:Young Adult
chemical_list: D003062:Codon; D009195:Peroxidase