title: Unprecedented deoxygenation at C-7 of the ansamitocin core during mutasynthetic biotransformations.
abstract: We describe the unprecedented formation of six ansamitocin derivatives that are deoxygenated at C-7 of the ansamitocin core, obtained during fermentation experiments by employing a variety of Actinosynnema pretiosum mutants and mutasynthetic approaches. We suggest that the formation of these derivatives is based on elimination at C-7/C-8 followed by reduction(s) of the intermediate enone. In bioactivity tests, only ansamitocin derivatives bearing an ester side chain at C-3 showed strong antiproliferative activity.
authors.affiliation: Institute of Organic Chemistry and Center of Biomolecular Drug Research (BMWZ), Leibniz University Hannover, Schneiderberg 1b, 30167 Hannover, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Knobloch
authors.forename: Gerald
authors.initials: G
authors.lastname: Dräger
authors.forename: Wera
authors.initials: W
authors.lastname: Collisi
authors.forename: Florenz
authors.initials: F
authors.lastname: Sasse
authors.forename: Andreas
authors.initials: A
authors.lastname: Kirschning