title: Osteogenic response of human adipose-derived stem cells to BMP-6, VEGF, and combined VEGF plus BMP-6 in vitro.
abstract: Exogenous addition of three factors-mesenchymal stem cells (MSCs), vascular endothelial growth factor (VEGF), and bone morphogenetic proteins (BMPs)-has proven to be more beneficial than delivery of any single factor for fracture repair in animal models. We studied the osteogenic differentiation of human adipose-derived stem cells (hADSCs) in the presence of VEGF, BMP-6, or VEGF plus BMP-6 to better understand their enhancement of osteoblastic differentiation of MSCs. The VEGF plus BMP-6 group demonstrated an additive effect on the enhancement of mineralization and expression of ALP and Msx2 genes. Unlike VEGF or BMP-6 alone, the combination of VEGF and BMP-6 significantly enhanced the expression of COL1A1, osterix, and Dlx5 genes. The data indicate that a cross-talk between VEGF and BMP-6 signaling pathways enhances osteogenic differentiation of hADSCs.
authors.affiliation: Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA 22908, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Vedavathi
authors.initials: V
authors.lastname: Madhu
authors.forename: Abhijit S
authors.initials: AS
authors.lastname: Dighe
authors.forename: James N
authors.initials: JN
authors.lastname: Irvine
authors.forename: Quanjun
authors.initials: Q
authors.lastname: Cui
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D055418:Bone Morphogenetic Protein 6 / Q000378:metabolism* / Q000494:pharmacology; D016723:Bone Remodeling; D002460:Cell Line; D054425:Chemokine CCL27 / Q000096:biosynthesis; D024042:Collagen Type I / Q000096:biosynthesis; D000090602:Collagen Type I, alpha 1 Chain; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000096:biosynthesis; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D010012:Osteogenesis; D000076182:Sp7 Transcription Factor; D013234:Stem Cells; D014157:Transcription Factors / Q000096:biosynthesis; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism* / Q000494:pharmacology
chemical_list: C525816:BMP6 protein, human; D055418:Bone Morphogenetic Protein 6; C492276:CCL27 protein, human; D054425:Chemokine CCL27; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; C100596:DLX5 protein, human; D018398:Homeodomain Proteins; C084119:MSX2 protein; D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A