title: [Mechanisms for inhibition effects of polypeptide extract from scorpion venom (PESV) on proliferation of A549 cell lines in vitro].
abstract: OBJECTIVE
  To investigate the mechanisms for inhibition effects of PESV on proliferation of non-small cell lung cancer cell line A549.
  
  
  METHODS
  MTT was used to observe cell growth and proliferation of A549 at different concentrations of PESV. Flow cytometry (FCM) was applied to analyze cell cycle distribution. Immunocytochemistry and western blot assay was recruited to detect the expression of VEGF, HIF-1alpha, PTEN after the intervention of PESV.
  
  
  RESULTS
  A549 cells may be arrested mainly in G0/G1 phase and cell proliferation was significantly inhibited (P < 0.01) after PESV intervention in a certain range of concentration. PESV can significantly reduce the expression of HIF-1alpha,VEGF and increase the expression of PTEN.
  
  
  CONCLUSIONS
  PESV can block cell cycle and inhibit angiogenesis directly to inhibit cell proliferation of non-small cell lung cancer cell line A549 mainly through reducing the expression of HIF-1alpha, VEGF and increasing the expression of PTEN.
authors.affiliation: Department of Pathology, Institute of Basic Medcine, Shandong Academy of Medical Science, Ji'nan 250062, China. xiaohui_12345777@sina.com
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.forename: Zhaopeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Yueying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Jia
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology*; D012604:Scorpion Venoms / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D010455:Peptides; D012604:Scorpion Venoms; D042461:Vascular Endothelial Growth Factor A; D051059:PTEN Phosphohydrolase