title: [Effect of gallnut extract on nasopharyngeal carcinoma 5-8F cells and its mechanism].
abstract: OBJECTIVE
  To determine the biological activity of ellagic acid extracted from gallnut against nasopharyngeal carcinoma and its molecular mechanism.
  
  
  METHODS
  Nasopharyngeal carcinoma 5-8F cells were treated with 2, 4, 6 μg/mL ellagic acid for 48 h in vitro. The cell proliferation and cell apoptosis were analyzed by MTT and Hoechst33258 stain. The cell cycle and protein expression were measured by flow cytometry and Western blot.
  
  
  RESULTS
  Ellagic acid inhibited the proliferation of 5-8F cells. The inhibition rates were (29.35±4.95)%, (53.32 ±4.44)% and ( 61.75 + 6.93)%, respectively, with significant difference from the control group (P<0.01). S phase cells in the experimental groups were (25.47±0.74)%, (28.08±1.41)% and (35.49±0.66)%, respectively, with significant difference (P<0.01) from the control group (21.26±0.70)%. Cells in the experimental groups showed nuclear pyknosis, karyorrhexis and poptotic cell morphology. The expression of COX-2 and stathmin in 5-8F cells was down-regulated with increased drug concentration.
  
  
  CONCLUSIONS
  Ellagic acid extracted from gallnut has activity against nasopharyngeal carcinoma cells, and its mechanism may be related to down-regulated expression of COX-2 and stathmin.
authors.affiliation: Department of Pathology and Pathophysiology, Guilin Medical College, Guilin Guangxi, China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Xiang
authors.forename: Caiwen
authors.initials: C
authors.lastname: Fan
authors.forename: Shengjun
authors.initials: S
authors.lastname: Xiao
authors.forename: Hongjie
authors.initials: H
authors.lastname: Pan
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D004610:Ellagic Acid / Q000494:pharmacology*; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D010936:Plant Extracts / Q000494:pharmacology*; D050777:Stathmin / Q000235:genetics / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; C493995:STMN1 protein, human; D050777:Stathmin; D004610:Ellagic Acid; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human