title: Effect of different liver resection methods on liver damage and regeneration factors VEGF and FGF-2 in mice.
abstract: BACKGROUND
  Different approaches to study liver regeneration in murine models have been proposed. We investigated the effect of different liver resection models on liver damage and regeneration parameters in mice.
  
  
  METHODS
  We compared the technical aspect of the 2 most commonly used techniques of 50% and 70% liver resection. Liver damage, as determined by the change in serum alanine aminotransferase and aspartate aminotransferase, as well as the regeneration parameters VEGF and FGF-2 were analyzed at 6 time points. A postoperative vitality score was introduced.
  
  
  RESULTS
  Cholestasis was not observed for either technique. Both resection techniques resulted in full weight recovery of the liver after 240 hours, with no significant difference between sham and resection groups. Postoperative animal morbidity and total protein levels did not differ significantly for either method, indicating early and full functional recovery. However, comparing the mitogenic growth factors FGF-2 and VEGF, a significant increase in serum levels and, therefore, increased growth stimulus, was shown in the extended resection group.
  
  
  CONCLUSIONS
  Extended resection led to a greater response in growth factor expression. This finding is important since it shows that growth factor response differs acdording to the extent of resection. We have demonstrated the need to standardize murine hepatic resection models to adequately compare the resulting liver damage.
authors.affiliation: The Department of Surgery, Medical Faculty Mannheim, University Medical Centre Mannheim, Heidelberg University, Heidelberg, Germany.
authors.forename: Roderich
authors.initials: R
authors.lastname: Bönninghoff
authors.forename: Kay
authors.initials: K
authors.lastname: Schwenke
authors.forename: Michael
authors.initials: M
authors.lastname: Keese
authors.forename: Richard
authors.initials: R
authors.lastname: Magdeburg
authors.forename: Hinrich
authors.initials: H
authors.lastname: Bitter-Suermann
authors.forename: Mirko
authors.initials: M
authors.lastname: Otto
authors.forename: Till
authors.initials: T
authors.lastname: Hasenberg
authors.forename: Stefan
authors.initials: S
authors.lastname: Post
authors.forename: Jörg
authors.initials: J
authors.lastname: Sturm
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006498:Hepatectomy / Q000009:adverse effects* / Q000379:methods*; D008099:Liver / Q000201:enzymology / Q000293:injuries* / Q000378:metabolism*; D008107:Liver Diseases / Q000378:metabolism / Q000601:surgery; D008115:Liver Regeneration; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009068:Movement; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D042461:Vascular Endothelial Growth Factor A; D016222:Fibroblast Growth Factor 2; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D001663:Bilirubin