title: Development of latex agglutination test for rapid detection of antibodies against Bovine herpesvirus 1 and Bovine herpesvirus 5 in cattle.
abstract: A latex agglutination test (LAT) based on sensitized polystyrene beads with inactivated Bovine herpesvirus 1 (BHV-1) particles was developed to detect serum antibodies against BHV-1 and Bovine herpesvirus 5 (BHV-5). Compared with a virus neutralization test using 252 serum samples, the sensitivity and specificity were 94.7% and 97.5%, respectively. Finally, 512 clinical serum samples from 3 major cattle-breeding provinces were monitored, and the overall positive rate was 34.57% (95% confidence interval: 30.45-38.69%). The results suggest that LAT has the potential to be a rapid method for the diagnosis of BHV-1 and BHV-5 infection in cattle herds.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fan
authors.forename: Lan
authors.initials: L
authors.lastname: Yao
authors.forename: Ming
authors.initials: M
authors.lastname: Ding
authors.forename: Dan-Jing
authors.initials: DJ
authors.lastname: Wang
authors.forename: Huan-Chun
authors.initials: HC
authors.lastname: Chen
authors.forename: Zheng-Fei
authors.initials: ZF
authors.lastname: Liu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D018792:Encephalitis, Viral / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary / Q000821:virology; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D025881:Herpesvirus 5, Bovine / Q000276:immunology*; D007241:Infectious Bovine Rhinotracheitis / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D007841:Latex Fixation Tests / Q000379:methods / Q000662:veterinary*; D008590:Meningoencephalitis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D009500:Neutralization Tests; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral