title: [Identification and secondary metabolites of endophytic fungal strain PR35 from Paeonia delavayi].
abstract: OBJECTIVE
  To identify the endophytic fungal strain PR35 separated from Paeonia delavayi and study chemical constituents of its secondary metabolites.
  
  
  METHODS
  The fungal strain PR35 was identified by morphological observation and ITS rDNA sequence analysis. Various chromatographic methods were adopted to separate and purify its secondary metabolites, and their structures were identified by physiochemical properties and spectral data
  
  
  RESULTS
  The fungal strain PR35 was identified as Trichoderma longibrachiatum. Five compounds were separated from fermentation products of fungal strain PR35 and identified as 1-(2,6-dihydroxyphenyl)-3-hydroxybutan-1-one (1), 1-(2,6-dihydroxypheny) propan-1-one (2), 1-(2,4,6-trihydroxyphenyl) butan-1-one (3), 4-methoxy-1-naphthol (4), and cerevisterol (5). Among them, compounds 1-3 showed notable antifungal activities against Botrytis cinerea, Fusarium avenaceum and Hormodendrum compactum.
  
  
  CONCLUSIONS
  The endophytic fungus T. longibrachiatum was separated from the plant P. delavayi for the first time. Five compounds were first separated from endophytic fungus of P. delavayi. Among them, compound 4 was separated from microbial fermentation products for the first time.
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Yunnan Institute of Microbiology, Yunnan University, Ministry of Education, Kunming 650091, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Hu
authors.forename: Jiao
authors.initials: J
authors.lastname: Wang
authors.forename: Cuiping
authors.initials: C
authors.lastname: Miao
authors.forename: Qicun
authors.initials: Q
authors.lastname: Xuan
authors.forename: Yingzhe
authors.initials: Y
authors.lastname: Zhai
authors.forename: Fei
authors.initials: F
authors.lastname: Song
authors.forename: Youwei
authors.initials: Y
authors.lastname: Chen
authors.forename: Shaohua
authors.initials: S
authors.lastname: Wu
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D029596:Paeonia / Q000382:microbiology*; D010802:Phylogeny; D014242:Trichoderma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D021901:DNA, Intergenic