title: Biocatalytic reactors based on ribonuclease A immobilized on macroporous monolithic supports.
abstract: Immobilized enzyme reactors (IMERs) produced by the covalent attachment of ribonuclease A to macroporous methacrylate-based monolithic supports using different experimental approaches are discussed and compared. Enzyme immobilization was carried out by direct covalent binding, as well as through attachment via a polymer spacer. The kinetic properties of an IMER operating in either recirculation mode or zonal elution mode were studied. Additionally, the effect of flow rate on the bioconversion efficiency of each IMER sample was examined.
authors.affiliation: Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Ponomareva
authors.forename: M V
authors.initials: MV
authors.lastname: Volokitina
authors.forename: D O
authors.initials: DO
authors.lastname: Vinokhodov
authors.forename: E G
authors.initials: EG
authors.lastname: Vlakh
authors.forename: T B
authors.initials: TB
authors.lastname: Tennikova
mesh_terms: D000818:Animals; D055162:Biocatalysis; D002417:Cattle; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007700:Kinetics; D016062:Porosity; D012117:Resins, Synthetic / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D012117:Resins, Synthetic; D012259:Ribonuclease, Pancreatic