title: The potential role of ozone in ameliorating the age-related biochemical changes in male rat cerebral cortex.
abstract: Controlled ozone (O(3)) administration is known to promote oxidative preconditioning and, thus, may reverse chronic oxidative stress that accompanies aging. Therefore, the present work was undertaken to study the potential role of O(3) in ameliorating certain age-related biochemical changes represented by impaired activities of inner mitochondrial membrane enzymes, compromised energy production and increased oxidative burden in male rat cerebral cortex. Prophylactic administration of O(3)-O(2) mixture to 3 month-old rats, at an intrarectal dose of 0.6 mg O(3) kg(-1) body weight twice/week for 3 months then once/week until the age of 15 months, normalized reduced glutathione content, adenosine triphosphate/adenosine diphosphate ratio, mitochondrial superoxide dismutase (SOD) and complex IV (cytochrome-c oxidase) activities, improved glutathione redox index (GSHRI), complex I (NADH-ubiquinone oxidoreductase) and mitochondrial nitric oxide synthase (mtNOS) activities, and attenuated the rise in malondialdehyde (MDA) and mitochondrial protein carbonyl levels. On the other hand, therapeutic administration of the same dose of O(3)-O(2) mixture to 14 month-old rats three times/week for 1 month, reduced mitochondrial protein carbonyl level only. Other favorable effects, including normalization of Na,K-adenosine triphosphatase (Na,K-ATPase) activity and reduction in lipofuscin level in the prophylactic group, as well as improvement in mitochondrial SOD and complex I activities with a decrease in total MDA level in the therapeutic group, were comparable to the effects observed in the corresponding O(2)-treated control groups. In conclusion, the present study revealed that prophylactic administration of O(3)-O(2) mixture provided better amelioration of age-related cerebrocortical alterations by combining the advantages of both O(3) and O(2) therapies.
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Cairo University, Kasr El-Ainy-Street, Cairo 11562, Egypt. nagwaibrahim99@yahoo.com
authors.forename: Nagwa Ibrahim
authors.initials: NI
authors.lastname: Shehata
authors.forename: Hanan Mohamed
authors.initials: HM
authors.lastname: Abd-Elgawad
authors.forename: Mohamed Nabil
authors.initials: MN
authors.lastname: Mawsouf
authors.forename: Amira Abd-Elmonem
authors.initials: AA
authors.lastname: Shaheen
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D042967:Electron Transport Complex I / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D008062:Lipofuscin / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010126:Ozone / Q000008:administration & dosage / Q000493:pharmacokinetics; D050050:Protein Carbonylation / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D008062:Lipofuscin; D008315:Malondialdehyde; D010126:Ozone; D019001:Nitric Oxide Synthase; D013482:Superoxide Dismutase; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I; D005978:Glutathione