title: [CA916798 gene participates in cisplatin resistance of human lung adenocarcinoma A549 cells through PI3K/AKT/mTOR pathway].
abstract: OBJECTIVE
  To observe the changes of CA916798 gene expression in human lung adenocarcinoma cell A549 and the multidrug-resistant cell line A549/CDDP after blocking the PI3K/AKT/mTOR pathway.
  
  
  METHODS
  Human lung adenocarcinoma cell lines A549 and A549/CDDP were treated with rapamycin and LY294002, and the cell growth changes in response to subsequent cisplatin treatment was observed; RT-PCR was performed to detect CA916798 mRNA expression in the treated cells.
  
  
  RESULTS
  Blocking PI3K/AKT/mTOR pathway with rapamycin and LY294002 significantly reduced drug resistance of both A549 and A549/CDDP cells to cisplatin and obviously decreased the expression of CA916798 gene mRNA (P<0.05).
  
  
  CONCLUSIONS
  CA9167981 gene is located downstream of the PI3K/AKT/mTOR pathway, which might be one of the mechanisms of CA916798 to cause cisplatin resistance in the tumor cells.
authors.affiliation: Department of Respiratory Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. qizhanzhong@sina.com
authors.forename: Zhanzhong
authors.initials: Z
authors.lastname: Qi
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Zhou
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*
chemical_list: D002945:Cisplatin; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; C546842:MTOR protein, human