title: Gene expression in breast muscle associated with feed efficiency in a single male broiler line using a chicken 44K microarray. II. Differentially expressed focus genes.
abstract: Global RNA expression in breast muscle obtained from a male broiler line phenotyped for high or low feed efficiency (FE) was investigated using microarray analysis. Microarray procedures and validation were reported previously. By using an overlay function of a software program (Ingenuity Pathway Analysis, IPA) in which canonical pathways are projected onto a set of genes, a subset of 27 differentially expressed focus genes were identified. Focus genes that were upregulated in the high FE phenotype were associated with important signal transduction pathways (Jnk, G-coupled, and retinoic acid) or in sensing cell energy status and stimulating energy production that would likely enhance growth and development of muscle tissue. In contrast, focus genes that were upregulated in the low FE muscle phenotype were associated with cytoskeletal architecture (e.g., actin-myosin filaments), fatty acid oxidation, growth factors, or ones that would likely be induced in response to oxidative stress. The results of this study provide additional information on gene expression and the cellular basis of feed efficiency in broilers.
authors.affiliation: Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA. wbottje@uark.edu
authors.forename: W G
authors.initials: WG
authors.lastname: Bottje
authors.forename: B-W
authors.initials: BW
authors.lastname: Kong
authors.forename: J J
authors.initials: JJ
authors.lastname: Song
authors.forename: J Y
authors.initials: JY
authors.lastname: Lee
authors.forename: B M
authors.initials: BM
authors.lastname: Hargis
authors.forename: K
authors.initials: K
authors.lastname: Lassiter
authors.forename: T
authors.initials: T
authors.lastname: Wing
authors.forename: J
authors.initials: J
authors.lastname: Hardiman
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000378:metabolism*; D004734:Energy Metabolism / Q000235:genetics* / Q000502:physiology; D020869:Gene Expression Profiling / Q000662:veterinary*; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D010641:Phenotype; D040081:Protein Array Analysis / Q000662:veterinary; D012313:RNA / Q000235:genetics / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; D012313:RNA