title: Differential roles of cyclin-dependent kinase 5 in tangential and radial migration of cerebellar granule cells.
abstract: The cerebellar granule cell is a unique neuron which undergoes tangential migration along axonal tracts and radial migration along glial fibers sequentially during postnatal development. Little is known about molecular bases of the differential kinetics of tangential and radial migration. Here we developed a time-lapse imaging assay for tangential migration of cerebellar granule cells, and investigated comparative contributions of cyclin-dependent kinase 5 (CDK5), a key regulator of neuronal migration, in tangential and radial migration of granule cells in vivo and in organotypic cultures. Overexpression of a dominant-negative form of CDK5 severely disrupted cell morphology and somal movement during radial migration, while it only moderately affected tangential migration. Dominant-negative inhibition of CDK5 induced formation of ectopic radial processes in granule cells in vivo which aberrantly elongated into the white matter in the cerebellum. Live imaging of granule cell migration in cerebellar slices revealed that CDK5 regulates not only nuclear migration but also centrosome movement during radial migration. These findings suggest a mode-specific function of CDK5 in neuronal migration.
authors.affiliation: Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto 606-8501, Japan. umeshima@icems.kyoto-u.ac.jp
authors.forename: Hiroki
authors.initials: H
authors.lastname: Umeshima
authors.forename: Mineko
authors.initials: M
authors.lastname: Kengaku
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002531:Cerebellum / Q000254:growth & development* / Q000378:metabolism*; D051360:Cyclin-Dependent Kinase 5 / Q000378:metabolism*; D018274:Electroporation; D005455:Fluorescent Antibody Technique; D018014:Gene Transfer Techniques; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D051360:Cyclin-Dependent Kinase 5