title: Weak intra-ring allosteric communications of the archaeal chaperonin thermosome revealed by normal mode analysis.
abstract: Chaperonins are molecular machines that use ATP-driven cycles to assist misfolded substrate proteins to reach the native state. During the functional cycle, these machines adopt distinct nucleotide-dependent conformational states, which reflect large-scale allosteric changes in individual subunits. Distinct allosteric kinetics has been described for the two chaperonin classes. Bacterial (group I) chaperonins, such as GroEL, undergo concerted subunit motions within each ring, whereas archaeal and eukaryotic chaperonins (group II) undergo sequential subunit motions. We study these distinct mechanisms through a comparative normal mode analysis of monomer and double-ring structures of the archaeal chaperonin thermosome and GroEL. We find that thermosome monomers of each type exhibit common low-frequency behavior of normal modes. The observed distinct higher-frequency modes are attributed to functional specialization of these subunit types. The thermosome double-ring structure has larger contribution from higher-frequency modes, as it is found in the GroEL case. We find that long-range intersubunit correlation of amino-acid pairs is weaker in the thermosome ring than in GroEL. Overall, our results indicate that distinct allosteric behavior of the two chaperonin classes originates from different wiring of individual subunits as well as of the intersubunit communications.
authors.affiliation: Department of Chemistry, Northern Kentucky University, Highland Heights, Kentucky, USA.
authors.forename: Manori
authors.initials: M
authors.lastname: Jayasinghe
authors.forename: Pooja
authors.initials: P
authors.lastname: Shrestha
authors.forename: Xiongwu
authors.initials: X
authors.lastname: Wu
authors.forename: Riina
authors.initials: R
authors.lastname: Tehver
authors.forename: George
authors.initials: G
authors.lastname: Stan
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000494:Allosteric Regulation; D019843:Archaeal Proteins / Q000737:chemistry* / Q000378:metabolism; D017017:Methanococcus; D008958:Models, Molecular; D009068:Movement; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D056405:Thermosomes / Q000737:chemistry* / Q000378:metabolism
chemical_list: D019843:Archaeal Proteins; D021122:Protein Subunits; D000255:Adenosine Triphosphate; D056405:Thermosomes