title: Complete genome sequence analysis of Japanese encephalitis virus isolated from a horse in India.
abstract: The complete genome of the Japanese encephalitis virus (JEV) strain JEV/eq/India/H225/2009(H225), isolated from an infected horse in India, was sequenced and compared to previously published JEV genomes. H225 genome was 10,977-nucleotides long, comprising a single ORF of 10,299-nucleotides, a 5'-UTR of 95 nucleotides and a 3'-UTR of 582 nucleotides. The H225 genome showed high levels of sequence identity with 47 fully sequenced JEV genomes, ranging from 99.3 % to 75.5 % for nucleotides and 99.2 % to 91.5 % for amino acid sequences. Phylogenetic analysis of the full-length sequence indicated that the H225 strain belongs to genotype III and is closely related to the Indian JEV strain Vellore P20778. A comparison of amino acids associated with neurovirulence in the E proteins and non-structural proteins of known virulent and attenuated JEV strains suggested H225 to be a highly virulent strain. This is the first report of whole-genome sequencing of a genotype III JEV genome isolated from equines.
authors.affiliation: Equine Health Unit, National Research Centre on Equines, Sirsa Road, Hisar, 125 001 Haryana, India.
authors.forename: Harisankar
authors.initials: H
authors.lastname: Singha
authors.forename: Baldev R
authors.initials: BR
authors.lastname: Gulati
authors.forename: Prabhat
authors.initials: P
authors.lastname: Kumar
authors.forename: Birendra K
authors.initials: BK
authors.lastname: Singh
authors.forename: Nitin
authors.initials: N
authors.lastname: Virmani
authors.forename: Raj K
authors.initials: RK
authors.lastname: Singh
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins