title: Effect of adsorbed fibronectin on the differential adhesion of osteoblast-like cells and Staphylococcus aureus with and without fibronectin-binding proteins.
abstract: The influence of fibronectin (Fn) coated surfaces patterned with poly(ethylene glycol) microgels having inter-gel spacings between 0.5 and 3.0 μm on the adhesion of Staphylococcus aureus strains with and without Fn-binding proteins and cellular adhesion/spreading was investigated. Quantitative force measurements between a S. aureus cell and a patterned surface showed that the adhesion force between the bacterium and the patterned surface increased substantially after Fn adsorption, regardless of the strain used, but decreased with decreasing inter-gel spacing. In flow-chamber experiments, the Fn-binding strain adhered at a higher rate after Fn adsorption than the strain lacking Fn-binding proteins. In both cases, the adhesion rates decreased with decreasing inter-gel spacing. Osteoblast-like cells could bind to patterned surfaces despite the microgels, and adsorbed Fn substantially amplified this effect. Even under highly non-adhesive conditions associated with closely spaced microgels, adsorbed Fn preserves a window of inter-gel spacing around 1 μm where the adhesion of staphylococcal cells is hindered while cells can still adhere and spread.
authors.affiliation: Department of Biomedical Engineering, W.J. Kolff Institute, University Medical Center and University of Groningen, The Netherlands.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Subbiahdoss
authors.forename: J
authors.initials: J
authors.lastname: de Vries
authors.forename: M
authors.initials: M
authors.lastname: Libera
authors.forename: H C
authors.initials: HC
authors.lastname: van der Mei
authors.forename: H J
authors.initials: HJ
authors.lastname: Busscher
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism*; D000327:Adsorption; D001422:Bacterial Adhesion / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D005353:Fibronectins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
chemical_list: D018829:Adhesins, Bacterial; D005353:Fibronectins; C078635:fibronectin-binding proteins, bacterial