title: Drosophila Psf2 has a role in chromosome condensation.
abstract: The condensation state of chromosomes is a critical parameter in multiple processes within the cell. Failures in the maintenance of appropriate condensation states may lead to genomic instability, mis-expression of genes, and a number of disease states. During cell proliferation, replication of DNA represents an ongoing challenge for chromosome packaging as DNA must be unpackaged for replication and then faithfully repackaged. An integral member of the DNA replication machinery is the GINS complex which has been shown to stabilize the CMG complex which is required for processivity of the Mcm2-7 helicase complex during S phase. Through examination of the phenotypes associated with a null mutation in Psf2, a member of the evolutionarily conserved GINS complex, we find that Drosophila Psf2 likely has a role in establishing chromosome condensation and that the defects associated with this mis-condensation impact M phase progression, genomic stability, and transcriptional regulation.
authors.affiliation: Department of Biology, East Carolina University, Greenville, NC 27858, USA.
authors.forename: Jeffrey P
authors.initials: JP
authors.lastname: Chmielewski
authors.forename: Laura
authors.initials: L
authors.lastname: Henderson
authors.forename: Charlotte M
authors.initials: CM
authors.lastname: Smith
authors.forename: Tim W
authors.initials: TW
authors.lastname: Christensen
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002455:Cell Division / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D059006:Chromosomes, Insect / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D062951:Endoreduplication; D005260:Female; D018628:Gene Dosage; D009154:Mutation; D010053:Ovary / Q000502:physiology; D059007:Polytene Chromosomes; D016196:S Phase / Q000235:genetics
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; C581312:Psf2 protein, Drosophila