title: Source of angiopoietin-2 in the sera of women during pregnancy.
abstract: Placental development requires coordinated angiogenesis regulated by multiple factors including angiopoietins. Previously we demonstrated that the concentration of angiopoietin-2 (Ang-2) in the sera of women rises markedly in pregnancy in early gestation. This increase is reduced in pregnancies subsequently complicated by intrauterine growth restriction (IUGR). We now show that the concentration of Ang-2, but not Ang-1, in maternal serum is increased during normal pregnancy, peaking at the end of the first trimester. We also demonstrate that a key source of the elevated Ang-2 levels during pregnancy is decidual endothelial cells (DECs) but not cytotrophoblasts. Secretion of Ang-2 by DECs relies on the release from intracellular stores and the synthesis of new Ang-2 protein and is regulated by serum factors at a translational level. Further studies on the role of Ang-2 during pregnancy are warranted as well as the evaluation of Ang-2 as a marker to predict adverse pregnancy outcomes.
authors.affiliation: Division of Perinatal Research, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, St Leonards, New South Wales, Australia.
authors.forename: C
authors.initials: C
authors.lastname: Woolnough
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: C Y
authors.initials: CY
authors.lastname: Kan
authors.forename: J M
authors.initials: JM
authors.lastname: Morris
authors.forename: V
authors.initials: V
authors.lastname: Tasevski
authors.forename: A W
authors.initials: AW
authors.lastname: Ashton
mesh_terms: D000328:Adult; D042683:Angiopoietin-1 / Q000097:blood*; D042702:Angiopoietin-2 / Q000097:blood*; D003656:Decidua / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005317:Fetal Growth Retardation / Q000097:blood*; D005786:Gene Expression Regulation; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D053552:Keratin-7 / Q000096:biosynthesis; D010920:Placenta / Q000378:metabolism*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis; D011247:Pregnancy; D011256:Pregnancy Outcome; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000166:cytology
chemical_list: D042683:Angiopoietin-1; D042702:Angiopoietin-2; D007074:Immunoglobulin G; D053552:Keratin-7; D019408:Platelet Endothelial Cell Adhesion Molecule-1