title: Potent effects of, and mechanisms for, modification of crosstalk between macrophages and adipocytes by lactobacilli.
abstract: The murine macrophage-like cell line J774.1 was treated with heat-killed cells of Lactobacillus GG (LGG) and L. gasseri TMC0356 (TMC 0356). Interleukin (IL)-6, IL-12, and tumor necrosis factor-α were profiled from the J774.1 cells using enzyme-linked immunosorbent assay methods. The conditioned medium from cultured J774.1 cells was transferred to the preadipocyte cell line 3T3-L1 (which is a mouse embryonic fibroblast-adipose-like cell line). Growth and differentiation of 3T3-L1 cells were monitored by analyzing lipid accumulation and expression of peroxisome proliferator-activated receptor (PPAR)-γ mRNA. The medium conditioned by 3T3-L1 cells was added to J774.1 cells and the cytokines in the supernatant analyzed. Compared with that of cells exposed to a PBS-conditioned medium, lipid accumulation in 3T3-L1 cells was significantly suppressed in a dose-dependent manner by each medium that had been conditioned with LGG and TMC0356. PPAR-γ mRNA expression in 3T3-L1 cells was also significantly downregulated (P < 0.01, P < 0.05, respectively). The conditioned medium of 3T3-L1 adipose phenotype significantly stimulated production of IL-6 and IL-12 in J774.1 cells treated with LGG and TMC0356. These results suggest that lactobacilli may suppress differentiation of preadipocytes through macrophage activation and alter the immune responses of macrophages to adipose cells.
authors.affiliation: Technical Research Laboratory, Takanashi Milk Products, Yokohama, Kanagawa 241-0023, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Miyazawa
authors.forename: Fang
authors.initials: F
authors.lastname: He
authors.forename: Kazutoyo
authors.initials: K
authors.lastname: Yoda
authors.forename: Masaru
authors.initials: M
authors.lastname: Hiramatsu
mesh_terms: D017667:Adipocytes / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D017077:Culture Media, Conditioned / Q000737:chemistry; D016207:Cytokines / Q000032:analysis; D020869:Gene Expression Profiling; D007778:Lactobacillus / Q000276:immunology*; D008055:Lipids / Q000032:analysis; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D047495:PPAR gamma / Q000096:biosynthesis
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines; D008055:Lipids; D047495:PPAR gamma