title: Genetic manipulation of a naturally competent bacterium, Helicobacter pylori.
abstract: Genetic manipulation of Helicobacter pylori facilitates characterization and functional analysis of individual H. pylori genes. This chapter discusses the methods involved in H. pylori chromosomal DNA isolation, mutagenesis of individual genes, and natural transformation.
authors.affiliation: Division of Gastroenterology, Department of Medicine, Vanderbilt University Medical Center, MRB IV 1030C MRB IV, Nashville, TN, USA. jennifer.noto@vanderbilt.edu
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Noto
authors.forename: Richard M
authors.initials: RM
authors.lastname: Peek
mesh_terms: D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005818:Genetic Engineering / Q000379:methods*; D016480:Helicobacter pylori / Q000235:genetics* / Q000502:physiology; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial