title: Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types.
abstract: The present study describes the genotypic distribution of rotaviruses (RVs) in an Indian bovine population with unexpectedly higher proportions of G3 alone or in combination of G8/G10. PCR-genotyping confirmed that 39.4% (13/33) of the prevalent RVs were the G3 type while 60.6% (20/33) were dual G3G10 or G3G8 types. P typing revealed that 93.9% (31/33) of the samples were P[11] while 6.1% (2/33) possessed a dual P[1]P[11] type.  Sequence analysis of the VP7 gene from G3 strains viz. B-46, 0970, and BR-133 showed that these strains had sequence identities of 90.5% to 100% with other bovine G3 strains. The highest identity (98.9% to 100%) was observed with RUBV3 bovine G3 strains from eastern India. The G3 strains (B-46, 0970, and BR-133) showed 97.5% to 98.8% sequence homologies with the Indian equine RV strain Erv-80. Phylogenetic analysis demonstrated that G3 strains clustered with bovine RUBV3 and J-63, and equine Erv-80 G3. Overall, these results confirmed that the incidence of infection by RVs with the G3 genotype and mixed genotypes in the bovine population was higher than previously predicted. This finding reinforces the importance of constantly monitoring circulating viral strains with the G3 genotype in future surveillance studies.
authors.affiliation: Division of Virology, Indian Veterinary Research Institute, Campus Mukteswar 263138, Uttarakhand, India. malikyps@yahoo.com
authors.forename: Yashpal S
authors.initials: YS
authors.lastname: Malik
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Sharma
authors.forename: Nirupama
authors.initials: N
authors.lastname: Vaid
authors.forename: Somendu
authors.initials: S
authors.lastname: Chakravarti
authors.forename: K M
authors.initials: KM
authors.lastname: Chandrashekar
authors.forename: Sanjay S
authors.initials: SS
authors.lastname: Basera
authors.forename: Rashmi
authors.initials: R
authors.lastname: Singh
authors.lastname: Minakshi
authors.forename: Gaya
authors.initials: G
authors.lastname: Prasad
authors.forename: Baldev R
authors.initials: BR
authors.lastname: Gulati
authors.forename: Kiren N
authors.initials: KN
authors.lastname: Bhilegaonkar
authors.forename: Awadh B
authors.initials: AB
authors.lastname: Pandey
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D003889:Desert Climate; D005243:Feces / Q000821:virology; D005838:Genotype; D007194:India / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D020539:Sequence Analysis, Protein / Q000662:veterinary; D017423:Sequence Analysis, RNA / Q000662:veterinary; D017385:Sequence Homology; D014329:Tropical Climate
chemical_list: D012367:RNA, Viral