title: Butanol production from hemicellulosic hydrolysate of corn fiber by a Clostridium beijerinckii mutant with high inhibitor-tolerance.
abstract: A Clostridium beijerinckii mutant RT66 with considerable inhibitor-tolerance obtained by continuous culture was used for butanol production from non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid (SAHHC). In fed-batch fermentation, 1.8L of diluted SAHHC containing 10 g/L of reducing sugar was provided during the acidogenic phase and 0.2L of concentrated SAHHC containing 300 g/L of reducing sugar was provided during the solventogenic phase. The mutant produced a total amount of solvents of 12.9 g/L, which consisted of 3.1 g/L of acetone, 9.3 g/L of butanol and 0.5 g/L of ethanol. A solvent yield of 0.35 g/g sugar and a productivity of 0.18 g/L h in 72 h were achieved. The remarkable inhibitor-tolerance of C. beijerinckii RT66 demonstrates that this may be an excellent strain for butanol production from ligocellulosic materials.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.forename: Ai-yong
authors.initials: AY
authors.lastname: He
authors.forename: Teng-fei
authors.initials: TF
authors.lastname: Du
authors.forename: Da-wei
authors.initials: DW
authors.lastname: Zhu
authors.forename: Da-feng
authors.initials: DF
authors.lastname: Liang
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Ping-kai
authors.initials: PK
authors.lastname: Ouyang
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D061249:Batch Cell Culture Techniques; D000440:Butanols / Q000378:metabolism*; D046970:Clostridium beijerinckii / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D009154:Mutation / Q000235:genetics*; D010636:Phenols / Q000633:toxicity*; D011134:Polysaccharides / Q000378:metabolism*; D012997:Solvents / Q000494:pharmacology; D013464:Sulfuric Acids / Q000494:pharmacology; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000440:Butanols; D003470:Culture Media; D010636:Phenols; D011134:Polysaccharides; D012997:Solvents; D013464:Sulfuric Acids; C007916:hemicellulose; C033158:sulfuric acid