title: Expression of Tax-interacting protein 1 (TIP-1) facilitates angiogenesis and tumor formation of human glioblastoma cells in nude mice.
abstract: Glioblastoma is the most common and fatal type of primary brain tumors featured with hyperplastic blood vessels. Here, we performed meta-analyses of published data and established a correlation between high TIP-1 expression levels and the poor prognosis of glioblastoma patients. Next, we explored the biological relevance of TIP-1 expression in the pathogenesis of glioblastoma. By using orthotopic and heterotopic mouse models of human glioblastomas, this study has characterized TIP-1 as one contributing factor to the tumor-driven angiogenesis. In vitro and in vivo functional assays, along with biochemical analyses with microarrays and antibody arrays, have demonstrated that TIP-1 utilizes multiple pathways including modulating fibronectin gene expression and uPA protein secretion, to establish or maintain a pro-angiogenic microenvironment within human glioblastoma. In conclusion, this work supports one hypothesis that TIP-1 represents a novel prognostic biomarker and a therapeutic target of human glioblastoma.
authors.affiliation: Department of Radiation Oncology, School of Medicine, Vanderbilt University, Nashville, TN 37232, USA.
authors.forename: Miaojun
authors.initials: M
authors.lastname: Han
authors.forename: Hailun
authors.initials: H
authors.lastname: Wang
authors.forename: Hua-Tang
authors.initials: HT
authors.lastname: Zhang
authors.forename: Zhaozhong
authors.initials: Z
authors.lastname: Han
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000098:blood supply* / Q000473:pathology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic; D015398:Signal Transduction
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C421249:TAX1BP3 protein, human