title: Comparative  fusion expression of maize SINAT5 in two different strains of Escherichia  coli.
abstract: SINAT5  is a plant E3 ligase that regulates auxin signaling and root morphogenesis by  ubiquitination of the NAC1 protein. Consequently, it may be a putative  regulator of aspects of plant development cycles that are controlled by auxin.  Efficient production, purification and correctly folded form of this protein  are important requirements for functional studies. We produced and  quantitatively compared fusion expression of the "maltose binding protein (mbp)-maize sinat5" construct in two different strains of Escherichia coli. One-step  purification of fused products gave about 33 mg protein/L bacterial cell  culture for E. coli TB1 cells and approximately 18 mg protein/L  bacterial cell culture for E. coli DH5α cells. Continuous expression of the  fused product and similarity of growth patterns were observed in both cultures.
authors.affiliation: Department of Molecular Biotechnology, Research Institute for Fundamental Sciences, University of Tabriz, Tabriz, Iran. aghz_bioch@yahoo.co.in
authors.forename: A
authors.initials: A
authors.lastname: Gholizadeh
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D058619:Maltose-Binding Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics; D010957:Plasmids / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D003313:Zea mays / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D058619:Maltose-Binding Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C466426:SINAT5 protein, Arabidopsis