title: Inter-laboratory assessment of flow cytometric monocyte HLA-DR expression in clinical samples.
abstract: BACKGROUND
  Diminished expression of human leukocyte antigen DR on circulating monocytes (mHLA-DR) is a reliable indicator of immunosuppression in critically ill patients, predictive of both adverse outcome and septic complications. The objective of the present work was to test, in an inter-laboratory clinical study, a standardized protocol for mHLA-DR measurement by flow cytometry.
  
  
  METHODS
  mHLA-DR was assessed in fresh whole blood according to a standardized staining protocol. Cells were analyzed on different flow cytometers (FC500, Navios, FACS Canto II) in different laboratories (Lyon and Grenoble). Results were expressed as numbers of antibodies bound per cell (AB/C).
  
  
  RESULTS
  Correlations between results were excellent (Pearson and interclass correlation coefficients > 0.98). Coefficients of variations for intra-assay precision ranged from 1.9 to 3.2%.
  
  
  CONCLUSIONS
  The present report highlights the robustness of this standardized flow cytometric protocol for mHLA-DR measurement in multicentric clinical studies.
authors.affiliation: Hospices Civils de Lyon, Hôpital Edouard Herriot, Laboratoire d'Immunologie, Lyon F-69003, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Demaret
authors.forename: Alexandre
authors.initials: A
authors.lastname: Walencik
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Jacob
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Timsit
authors.forename: Fabienne
authors.initials: F
authors.lastname: Venet
authors.forename: Alain
authors.initials: A
authors.lastname: Lepape
authors.forename: Guillaume
authors.initials: G
authors.lastname: Monneret
mesh_terms: D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D012772:Shock, Septic / Q000276:immunology; D016896:Treatment Outcome
chemical_list: D006684:HLA-DR Antigens