title: Preparative isolation and purification of harpagoside and angroside C from the root of Scrophularia ningpoensis Hemsley by high-speed counter-current chromatography.
abstract: In this study, the bioactive component harpagoside and angroside C in the root of Scrophularia ningpoensis Hemsley was simultaneously separated by high-speed counter-current chromatography (HSCCC). A two-phase solvent system containing chloroform/n-butanol/methanol/water (4:1:3:2, v/v/v/v) was selected following consideration of the partition coefficient of the target compound. The crude extract (200 mg) was loaded onto a 280-mL HSCCC column and yielded 22 mg harpagoside and 31 mg angroside C with the purity of higher than 98 and 98.5%, respectively. It is feasible to isolate active compounds harpagoside and angroside C from S. ningpoensis using HSCCC.
authors.affiliation: Chemistry Institute of Pharmaceutical Resources, College of Pharmaceutical Sciences, Chongqing Engineering Center of Pharmacodynamics Evaluation, Southwest University, Chongqing, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Tian
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ye
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Shang
authors.forename: Yafei
authors.initials: Y
authors.lastname: Deng
authors.forename: Kai
authors.initials: K
authors.lastname: He
authors.forename: Xuegang
authors.initials: X
authors.lastname: Li
mesh_terms: D003377:Countercurrent Distribution / Q000379:methods*; D006027:Glycosides / Q000032:analysis / Q000302:isolation & purification*; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D011714:Pyrans / Q000032:analysis / Q000302:isolation & purification*; D032266:Scrophularia / Q000737:chemistry*
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D011714:Pyrans; C578097:angroside C; C033249:harpagoside