title: AiiA quorum-sensing quenching controls proteolytic activity and biofilm formation by Enterobacter cloacae.
abstract: The aim of this work was to evaluate a quorum-quenching approach to identify functions regulated by quorum sensing in Enterobacter cloacae. We employed an aiiA transconjugant strain of E. cloacae that synthesizes a lactonase enzyme that hydrolyzes N-acyl homoserine lactone signaling molecules to compare bacterial phenotypes in the presence and absence of quorum signals. The aiiA-expressing strain displayed increased proteolytic activity and intensity of a milk-clotting reaction when compared to the wild-type strain. Although both strains growing on polystyrene plates in rich media and a minimal medium of salts formed biofilms, the wild-type strain exhibited a higher number of adhered cells. On the surface of stainless steel coupons that were submerged in culture media, the number of adhered cells of the wild type contained up to one log more cells compared with the aiiA transconjugant. However, after 48 h of incubation, there was no significant difference between the strains. The results demonstrated that the quorum-sensing system negatively regulates proteolytic activity and is likely involved in the early steps of biofilm formation by E. cloacae 067.
authors.affiliation: Department of Microbiology, Federal University of Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: Adriana
authors.initials: A
authors.lastname: dos Reis Ponce
authors.forename: Maurilio Lopes
authors.initials: ML
authors.lastname: Martins
authors.forename: Elza Fernandes
authors.initials: EF
authors.lastname: de Araujo
authors.forename: Hilário Cuquetto
authors.initials: HC
authors.lastname: Mantovani
authors.forename: Maria Cristina Dantas
authors.initials: MC
authors.lastname: Vanetti
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics / Q000378:metabolism; D003227:Conjugation, Genetic; D016972:Enterobacter cloacae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D011137:Polystyrenes; D059748:Proteolysis; D053038:Quorum Sensing; D013193:Stainless Steel
chemical_list: D054742:Acyl-Butyrolactones; D001426:Bacterial Proteins; D011137:Polystyrenes; D013193:Stainless Steel; D002265:Carboxylic Ester Hydrolases; C020579:gluconolactonase