title: Dopamine exerts activation-dependent modulation of spinal locomotor circuits in the neonatal mouse.
abstract: Monoamines can modulate the output of a variety of invertebrate and vertebrate networks, including the spinal cord networks that control walking. Here we examined the multiple changes in the output of locomotor networks induced by dopamine (DA). We found that DA can depress the activation of locomotor networks in the neonatal mouse spinal cord following ventral root stimulation. By examining disinhibited rhythms, where the Renshaw cell pathway was blocked, we found that DA depresses a putative recurrent excitatory pathway that projects onto rhythm-generating circuitry of the spinal cord. This depression was D(2) but not D(1) receptor dependent and was not due exclusively to depression of excitatory drive to motoneurons. Furthermore, the depression in excitation was not dependent on network activity. We next compared the modulatory effects of DA on network function by focusing on a serotonin and a N-methyl-dl-aspartate-evoked rhythm. In contrast to the depressive effects on a ventral root-evoked rhythm, we found that DA stabilized a drug-evoked rhythm, reduced the frequency of bursting, and increased amplitude. Overall, these data demonstrate that DA can potentiate network activity while at the same time reducing the gain of recurrent excitatory feedback loops from motoneurons onto the network.
authors.affiliation: Hotchkiss Brain Institute, Departments of Comparative Biology and Experimental Medicine, Physiology and Pharmacology, and Clinical Neurosciences, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Humphreys
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Whelan
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D004298:Dopamine / Q000494:pharmacology / Q000502:physiology*; D051379:Mice; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D013116:Spinal Cord / Q000187:drug effects / Q000502:physiology*; D013126:Spinal Nerve Roots / Q000502:physiology
chemical_list: D004298:Dopamine