title: Identification of a Rho family specific guanine nucleotide exchange factor, FLJ00018, as a novel actin-binding protein.
abstract: FLJ00018/PLEKHG2 is a guanine nucleotide exchange factor for the Rho family small GTPases. FLJ00018 is directly activated by heterotrimeric G protein Gβγ subunits. Using two-hybrid screening, we have identified non-muscle cytosolic actin as a binding partner of FLJ00018. We found that there were two actin-binding regions in FLJ00018 at the N-terminal region (150-283 amino acids) and at the C-terminal region (465-1386 amino acids). The overexpression of non-muscle cytosolic actin attenuated the FLJ00018-induced serum response element-dependent gene transcription. These results suggest that non-muscle cytosolic actin may be a negative regulator of FLJ00018 through its interaction with the Dbl homology domain.
authors.affiliation: United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu, Japan.
authors.forename: Katsuya
authors.initials: K
authors.lastname: Sato
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Handa
authors.forename: Masashi
authors.initials: M
authors.lastname: Kimura
authors.forename: Yukio
authors.initials: Y
authors.lastname: Okano
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nagaoka
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nagase
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueda
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D044387:GTP-Binding Protein beta Subunits / Q000235:genetics / Q000378:metabolism; D044388:GTP-Binding Protein gamma Subunits / Q000235:genetics / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D027602:Serum Response Element; D014158:Transcription, Genetic; D014162:Transfection; D020798:Two-Hybrid System Techniques
chemical_list: D000199:Actins; D044387:GTP-Binding Protein beta Subunits; D044388:GTP-Binding Protein gamma Subunits; D020662:Guanine Nucleotide Exchange Factors; C527535:PLEKHG2 protein, human