title: Construction and analysis of experimental DNA vaccines against megalocytivirus.
abstract: Iridoviruses are large double-stranded DNA viruses with icosahedral capsid. The Iridoviridae family contains five genera, one of which is Megalocytivirus. Megalocytivirus has emerged in recent years as an important pathogen to a wide range of marine and freshwater fish. In this study, we aimed at developing effective genetic vaccines against megalocytivirus affecting farmed fish in China. For this purpose, we constructed seven DNA vaccines based on seven genes of rock bream iridovirus isolate 1 from China (RBIV-C1), a megalocytivirus with a host range that includes Japanese flounder (Paralichthys olivaceus) and turbot (Scophthalmus maximus). The protective potentials of these vaccines were examined in a turbot model. The results showed that after vaccination via intramuscular injection, the vaccine plasmids were distributed in spleen, kidney, muscle, and liver, and transcription of the vaccine genes and production of the vaccine proteins were detected in these tissues. Following challenge with a lethal-dose of RBIV-C1, fish vaccinated with four of the seven DNA vaccines exhibited significantly higher levels of survival compared to control fish. Of these four protective DNA vaccines, pCN86, which is a plasmid that expresses an 86-residue viral protein, induced the highest protection. Immunological analysis showed that pCN86 was able to (i) stimulate the respiratory burst of head kidney macrophages at 14 d, 21 d, and 28 d post-vaccination, (ii) upregulate the expression of immune relevant genes involved in innate and adaptive immunity, and (iii) induce production of serum antibodies that, when incubated with RBIV-C1 before infection, significantly reduced viral loads in kidney and spleen following viral infection of turbot. Taken together, these results indicate that pCN86 is an effective DNA vaccine that may be used in the control of megalocytivirus-associated diseases in aquaculture.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Hu
authors.forename: Zhi-Zhong
authors.initials: ZZ
authors.lastname: Xiao
authors.forename: Yun
authors.initials: Y
authors.lastname: Sun
authors.forename: Li
authors.initials: L
authors.lastname: Sun
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002681:China; D017931:DNA Primers / Q000235:genetics; D004266:DNA Virus Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005393:Fish Diseases / Q000821:virology*; D005412:Flatfishes; D005786:Gene Expression Regulation / Q000276:immunology*; D060226:Head Kidney / Q000276:immunology / Q000821:virology; D007497:Iridoviridae / Q000276:immunology*; D010957:Plasmids / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D016897:Respiratory Burst / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000276:immunology / Q000821:virology; D013997:Time Factors; D019444:Vaccines, DNA / Q000096:biosynthesis* / Q000276:immunology*
chemical_list: D017931:DNA Primers; D019444:Vaccines, DNA