title: [Analysis on isofraxidin contained in Acanthopanax senticosus extracts and its metabolites by UPLC-Q-TOF-MS and automatic data processing technique].
abstract: OBJECTIVE
  To analyze isofraxidin and its metabolites in rat plasma, bile, urine and feces after the oral administration of A. senticosus extracts.
  
  
  METHODS
  After the oral administration of 325 mg x kg(-1) of A. senticosus extracts, rat blood samples were collected at 1, 1.5, 2, 4, 6 h and bile samples were collected during 0-12 h. The above samples were prepared by SPE. Their urine and feces samples were collected during 0-12 h and 12-24 h. These samples were analyzed by UPLC-Q-TOF-MS and processed using Metabolynx XS.
  
  
  RESULTS
  M0 was detected in rat plasma, bile, urine and feces, isofraxidin glucuronide (M1) was detected in rat plasma and bile, which was first reported as the metabolite of isofraxidin.
  
  
  CONCLUSIONS
  Isofraxidin can be metabolized in the form of glucuronidation in vivo and evacuated in the form of isofraxidin.
authors.affiliation: Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China. keji-liu@163.com
authors.forename: Shumin
authors.initials: S
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.forename: Fang
authors.initials: F
authors.lastname: Lu
authors.forename: Guangli
authors.initials: G
authors.lastname: Yan
authors.forename: Shunru
authors.initials: S
authors.lastname: Bao
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics*; D004365:Drugs, Chinese Herbal / Q000378:metabolism / Q000493:pharmacokinetics*; D028001:Eleutherococcus / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D017208:Rats, Wistar; D013223:Statistics as Topic
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; C008182:isofraxidin