title: Epidemiological and molecular characterization of rubella virus isolated in São Paulo, Brazil during 1997-2004.
abstract: Rubella virus (RV) infection during the early stages of pregnancy can lead to serious birth defects, known as the congenital rubella syndrome (CRS). In 2003, the Pan American Health Organization (PAHO) adopted a resolution calling for the elimination of rubella and the congenital rubella syndrome (CRS) in the Americas by the year 2010. Brazil will have implemented the recommended PAHO strategy for elimination and interruption of endemic rubella virus transmission. The characterization of genotypes during the final stages of rubella elimination is important for determining whether new rubella isolates represent endemic transmission or importations. Samples (blood, urine, cerebrospinal fluid, and throat swabs) collected from patients with symptoms suggestive of rubella infection in 1997-2004 were isolated in cell culture and genotyped. Twenty-eight sequences were analyzed and two genotypes were identified: 1a and 1G. The information reported in this paper will contribute to understanding the molecular epidemiology of RV in São Paulo, Brazil.
authors.affiliation: Instituto Adolfo Lutz-Núcleo de Doenças Respiratórias, São Paulo, Brazil. figueiredocris@uol.com.br
authors.forename: C A
authors.initials: CA
authors.lastname: Figueiredo
authors.forename: M I
authors.initials: MI
authors.lastname: Oliveira
authors.forename: S P
authors.initials: SP
authors.lastname: Curti
authors.forename: A M S
authors.initials: AM
authors.lastname: Afonso
authors.forename: A L
authors.initials: AL
authors.lastname: Frugis Yu
authors.forename: J
authors.initials: J
authors.lastname: Araújo
authors.forename: D B
authors.initials: DB
authors.lastname: Oliveira
authors.forename: E L
authors.initials: EL
authors.lastname: Durigon
mesh_terms: D000293:Adolescent; D000328:Adult; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D011247:Pregnancy; D012367:RNA, Viral / Q000235:genetics; D012189:Retrospective Studies; D012409:Rubella / Q000453:epidemiology* / Q000821:virology*; D012412:Rubella virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014776:Virus Cultivation; D055815:Young Adult
chemical_list: D012367:RNA, Viral