title: Friedelin and lanosterol from Garcinia prainiana stimulated glucose uptake and adipocytes differentiation in 3T3-L1 adipocytes.
abstract: Friedelin and lanosterol have been isolated from twigs of Garcinia prainiana. Their structures were elucidated by spectroscopic methods. The compounds were examined for their effects on 3T3-L1 adipocytes. In the MTT assay, it was found that the compounds had no cytotoxic effects up to 25 µM. Adipocyte differentiation analysis was carried out by Oil Red O staining method. In the presence of adipogenic cocktail (MDI), it was found that friedelin and lanosterol enhanced intracellular fat accumulation by 2.02 and 2.18-fold, respectively, compared with the vehicle-treated cells. Deoxyglucose uptake assay was used to examine the insulin sensitivity of adipocytes in the presence of the compounds. It was found that friedelin was able to stimulate glucose uptake up to 1.8-fold compared with insulin-treated cells. It was suggested that friedelin and lanosterol may be beneficial to mimic insulin action that would be useful in the treatment of diabetes type 2 patients.
authors.affiliation: Department of Chemistry, Faculty of Science, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Pahang, Malaysia.
authors.forename: Deny
authors.initials: D
authors.lastname: Susanti
authors.forename: Mohamed Zaffar Ali Mohamed
authors.initials: MZ
authors.lastname: Amiroudine
authors.forename: Mohamad Fazlin
authors.initials: MF
authors.lastname: Rezali
authors.forename: Muhammad
authors.initials: M
authors.lastname: Taher
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D029760:Garcinia / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism*; D007810:Lanosterol / Q000494:pharmacology*; D008297:Male; D051379:Mice; D014315:Triterpenes / Q000494:pharmacology*; D000094042:Lupanes
chemical_list: D005947:Glucose; D007810:Lanosterol; D014315:Triterpenes; C060796:friedelin; D000094042:Lupanes