title: Synthesis of nylon 4 from gamma-aminobutyrate (GABA) produced by recombinant Escherichia coli.
abstract: In this study, we developed recombinant Escherichia coli strains expressing Lactococcus lactis subsp. lactis Il1403 glutamate decarboxylase (GadB) for the production of GABA from glutamate monosodium salt (MSG). Syntheses of GABA from MSG were examined by employing recombinant E. coli XL1-Blue as a whole cell biocatalyst in buffer solution. By increasing the concentration of E. coli XL1-Blue expressing GadB from the OD₆₀₀ of 2-10, the concentration and conversion yield of GABA produced from 10 g/L of MSG could be increased from 4.3 to 4.8 g/L and from 70 to 78 %, respectively. Furthermore, E. coli XL1-Blue expressing GadB highly concentrated to the OD₆₀₀ of 100 produced 76.2 g/L of GABA from 200 g/L of MSG with 62.4 % of GABA yield. Finally, nylon 4 could be synthesized by the bulk polymerization using 2-pyrrolidone that was prepared from microbially synthesized GABA by the reaction with Al₂O₃ as catalyst in toluene with the yield of 96 %.
authors.affiliation: Department of Environmental Engineering and Energy-Undergraduate Program, Myongji University, San 38-2, Nam-dong, Cheoin-gu, Yongin-si, Gyeonggido 449-728, Republic of Korea.
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Won
authors.initials: W
authors.lastname: Noh
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Oh
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Bong Keun
authors.initials: BK
authors.lastname: Song
authors.forename: Kwang Myung
authors.initials: KM
authors.lastname: Cho
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Jonggeon
authors.initials: J
authors.lastname: Jegal
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D009757:Nylons / Q000378:metabolism*; D011995:Recombination, Genetic; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D009757:Nylons; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase