title: Chemotherapy control by breath profile with application of SPME-GC/MS method.
abstract: Chemotherapy used as a treatment against lung cancer has influence on metabolic processes occurring in healthy cells. The changes of biochemical pathways proceeded inside cells might be observed in expired air. In the experiment, breath analysis was carried out before and after anticancer therapy. Expired air samples were collected from 22 patients with a biopsy confirmed lung cancer. Volatile organic compounds present in breath were analyzed by gas chromatography/mass spectrometry. For enrichment of analytes solid-phase microextraction technique was applied. Eight fibers covered by different sorbents were tested. Carboxen-polydimethylsiloxane fiber revealed the highest extraction efficiency in relation to analytes in breath. The data showed that cytostatic drugs increase the concentration of acetone and isoprene in the breath collected after chemotherapy. Volatile metabolites of administrated drugs were not identified in expired air.
authors.affiliation: Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ulanowska
authors.forename: Ewa
authors.initials: E
authors.lastname: Trawińska
authors.forename: Piotr
authors.initials: P
authors.lastname: Sawrycki
authors.forename: Bogusław
authors.initials: B
authors.lastname: Buszewski
mesh_terms: D000368:Aged; D001944:Breath Tests / Q000379:methods*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D052617:Solid Phase Microextraction / Q000379:methods*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D055549:Volatile Organic Compounds