title: Estimation of the quantity of bacteria encapsulated in Lentikats Biocatalyst via phospholipid fatty acids content: a preliminary study.
abstract: The content of phospholipid fatty acids (PLFA) was determined in samples of polyvinyl alcohol lenses (Lentikats Biocatalyst, LB) with encapsulated Paracoccus denitrificans withdrawn during long-term denitrification experiments. The total PLFA content correlated highly with specific denitrification activities of LB as well as biomass estimation based on image analyses of microscopic photos. The results confirmed the applicability of PLFA determination for estimation of the amount of living encapsulated microbial biomass during biotechnological applications.
authors.affiliation: Jan Evangelista Purkyně University, Králova Výšina, Ústí nad Labem, Czech Republic. josef.trogl@ujep.cz
authors.forename: Josef
authors.initials: J
authors.lastname: Trögl
authors.forename: Ivana
authors.initials: I
authors.lastname: Jirková
authors.forename: Petra
authors.initials: P
authors.lastname: Zemánková
authors.forename: Věra
authors.initials: V
authors.lastname: Pilařová
authors.forename: Petra
authors.initials: P
authors.lastname: Dáňová
authors.forename: Jana
authors.initials: J
authors.lastname: Pavlorková
authors.forename: Pavel
authors.initials: P
authors.lastname: Kuráň
authors.forename: Jan
authors.initials: J
authors.lastname: Popelka
authors.forename: Lucie
authors.initials: L
authors.lastname: Křiklavová
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D058491:Bacterial Load; D001709:Biotechnology / Q000379:methods*; D004339:Drug Compounding / Q000379:methods; D005227:Fatty Acids / Q000378:metabolism*; D050296:Microbial Viability; D010743:Phospholipids / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D010743:Phospholipids