title: Impact of sorbic acid on germination and outgrowth heterogeneity of Bacillus cereus ATCC 14579 spores.
abstract: Population heterogeneity complicates the predictability of the outgrowth kinetics of individual spores. Flow cytometry sorting and monitoring of the germination and outgrowth of single dormant spores allowed the quantification of acid-induced spore population heterogeneity at pH 5.5 and in the presence of sorbic acid. This showed that germination efficiency was not a good predictor for heterogeneity in final outgrowth.
authors.affiliation: Wageningen University, Laboratory of Food Microbiology, Wageningen, the Netherlands. Heidy.denBesten@wur.nl
authors.forename: Heidy M W
authors.initials: HM
authors.lastname: den Besten
authors.forename: Clint C J
authors.initials: CC
authors.lastname: van Melis
authors.forename: Jan Willem
authors.initials: JW
authors.lastname: Sanders
authors.forename: Masja N
authors.initials: MN
authors.lastname: Nierop Groot
authors.forename: Tjakko
authors.initials: T
authors.lastname: Abee
mesh_terms: D001409:Bacillus cereus / Q000254:growth & development* / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005434:Flow Cytometry; D006863:Hydrogen-Ion Concentration; D013011:Sorbic Acid / Q000378:metabolism*; D013171:Spores, Bacterial / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D003470:Culture Media; D013011:Sorbic Acid