title: Optimized hyaluronic acid-hydrogel design and culture conditions for preservation of mesenchymal stem cell properties.
abstract: A novel approach that preserved most mesenchymal stem cell (MSC) characteristics was developed using MSC encapsulation in a hydrogel based on hyaluronic acid (HA). An optimized HA-hydrogel composition, whose characteristics were assessed by scanning electron microscopy and viscoelastic property analyses, as well as the more favorable MSC seeding density, was established. These optimal three-dimensional MSC culture conditions allowed morphological cell remodeling, maintained the expression of stem cell markers over 28 days of culture, and preserved MSC differentiation plasticity. In addition, MSCs in HA-hydrogel submitted for 7 days to mechanical constraint that aimed at mimicking in vivo cardiac beat displayed enhanced cell survival by more than 40% compared to static culture conditions. Thus, the optimized HA-based hydrogel provides a niche for MSCs, which preserves their properties and opens ways for cell therapy, in particular in aortic repair medicine.
authors.affiliation: CNRS EAC 4396, Centre de Recherches Chirurgicales, Faculté de médecine, Université Paris-Est Créteil Val de Marne, Créteil cedex, France.
authors.forename: Faïza
authors.initials: F
authors.lastname: Mohand-Kaci
authors.forename: Nabila
authors.initials: N
authors.lastname: Assoul
authors.forename: Isabelle
authors.initials: I
authors.lastname: Martelly
authors.forename: Eric
authors.initials: E
authors.lastname: Allaire
authors.forename: Mustapha
authors.initials: M
authors.lastname: Zidi
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005434:Flow Cytometry; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011916:Rats, Inbred F344; D012212:Rheology
chemical_list: D020100:Hydrogels; D006820:Hyaluronic Acid