title: Molecular characterization of Indian sugarcane streak mosaic virus isolate.
abstract: Sugarcane streak mosaic virus (SCSMV), a member of the genus Poacevirus, family Potyviridae, is an important viral pathogen affecting sugarcane cultivation in India. The complete nucleotide sequence of a SCSMV isolate from India (SCSMV-IND) was determined. The linear, assembled, single-stranded positive-sense RNA genome of SCSMV-IND was 9,786 nucleotides in length (excluding the poly (A) tail) and encoded a polyprotein of 3,131 amino acid residues. The genome of SCSMV-IND shared high degree of sequence identity with SCSMV-PAK (93 % at nucleotide and 97 % at amino acid), and shared only 81 % nucleotide and 94 % amino acid identities with all the four SCSMV isolates (SCSMV-JP1, -JP2, -ID, and -THA). Phylogenetic tree analysis of the complete genome sequences of SCSMV isolates revealed that they can be clustered into two groups. SCSMV-IND and -AP isolates showed 18 % (nucleotide) divergence within the highly conserved 3' partial genome, suggesting a high level of genetic diversity among the Indian SCSMV isolates.
authors.affiliation: Sugarcane Breeding Institute Regional Centre, Karnal, Haryana, India.
authors.forename: B
authors.initials: B
authors.lastname: Parameswari
authors.forename: K
authors.initials: K
authors.lastname: Bagyalakshmi
authors.forename: R
authors.initials: R
authors.lastname: Viswanathan
authors.forename: C
authors.initials: C
authors.lastname: Chinnaraja
mesh_terms: D016000:Cluster Analysis; D016679:Genome, Viral; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D020815:Polyproteins / Q000235:genetics; D019176:Potyviridae / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D031786:Saccharum / Q000821:virology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D020815:Polyproteins; D012367:RNA, Viral