title: Physiological responses of Tillandsia albida (Bromeliaceae) to long-term foliar metal application.
abstract: The impact of 2-month foliar application of cadmium, nickel and their combination (10 μM) on Tillandsia albida was studied. Cadmium caused damage of tissue but assimilation pigments were depressed in Cd+Ni variant only. Stress-related parameters (ROS and peroxidase activities) were elevated by Cd and Cd+Ni while MDA content remained unaffected. Free amino acids accumulated the most in Ni alone but soluble proteins were not influenced. Among phenolic acids, mainly vanillin contributed to increase of their sum in all variants while soluble phenols even decreased in Cd+Ni and flavonols slightly increased in Cd variants. Phenolic enzymes showed negligible responses to almost all treatments. Mineral nutrients (K, Ca, Na, Mg, Fe, and Zn) were not affected by metal application but N content increased. Total Cd or Ni amounts reached over 400 μg g(-1) DW and were not affected if metal alone and combined treatment is compared while absorbed content differed (ca. 50% of total Cd was absorbed while almost all Ni was absorbed). These data indicate tolerance of T. albida to foliar metal application and together with strong xerophytic morphology, use for environmental studies is recommended.
authors.affiliation: Institute of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemědělská 1, 613 00 Brno, Czech Republic. jozkovacik@yahoo.com
authors.forename: Jozef
authors.initials: J
authors.lastname: Kováčik
authors.forename: Bořivoj
authors.initials: B
authors.lastname: Klejdus
authors.forename: František
authors.initials: F
authors.lastname: Stork
authors.forename: Josef
authors.initials: J
authors.lastname: Hedbavny
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D002338:Carotenoids / Q000378:metabolism; D004156:Catechol Oxidase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D004785:Environmental Pollutants / Q000633:toxicity*; D005419:Flavonoids / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009532:Nickel / Q000633:toxicity*; D010636:Phenols / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D031232:Tillandsia / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004785:Environmental Pollutants; D005419:Flavonoids; D010636:Phenols; D010940:Plant Proteins; D002104:Cadmium; D013481:Superoxides; D002734:Chlorophyll; D002338:Carotenoids; D008315:Malondialdehyde; D009532:Nickel; D006861:Hydrogen Peroxide; D004156:Catechol Oxidase; D010650:Phenylalanine Ammonia-Lyase; D005978:Glutathione