title: [Key effect genes responding to nerve injury identified by gene ontology and computer pattern recognition].
abstract: OBJECTIVE
  In order to screen out important genes from large gene data of gene microarray after nerve injury, we combine gene ontology (GO) method and computer pattern recognition technology to find key genes responding to nerve injury, and then verify one of these screened-out genes.
  
  
  METHODS
  Data mining and gene ontology analysis of gene chip data GSE26350 was carried out through MATLAB software. Cd44 was selected from screened-out key gene molecular spectrum by comparing genes' different GO terms and positions on score map of principal component. Function interferences were employed to influence the normal binding of Cd44 and one of its ligands, chondroitin sulfate C (CSC), to observe neurite extension.
  
  
  RESULTS
  Gene ontology analysis showed that the first genes on score map (marked by red *) mainly distributed in molecular transducer activity, receptor activity, protein binding et al molecular function GO terms. Cd44 is one of six effector protein genes, and attracted us with its function diversity. After adding different reagents into the medium to interfere the normal binding of CSC and Cd44, varying-degree remissions of CSC's inhibition on neurite extension were observed.
  
  
  CONCLUSIONS
  CSC can inhibit neurite extension through binding Cd44 on the neuron membrane. This verifies that important genes in given physiological processes can be identified by gene ontology analysis of gene chip data.
authors.affiliation: Histology and Embryology Department, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Pan
authors.forename: Jin
authors.initials: J
authors.lastname: Peng
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.forename: Hao
authors.initials: H
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002809:Chondroitin Sulfates / Q000378:metabolism; D019295:Computational Biology / Q000379:methods*; D057225:Data Mining / Q000379:methods; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D018960:Hyaluronan Receptors / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010363:Pattern Recognition, Automated; D059348:Peripheral Nerve Injuries / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries
chemical_list: D018960:Hyaluronan Receptors; D002809:Chondroitin Sulfates