title: Sunflower oil supplementation has proinflammatory effects and does not reverse insulin resistance in obesity induced by high-fat diet in C57BL/6 mice.
abstract: High consumption of polyunsaturated fatty acids, such as sunflower oil has been associated to beneficial effects in plasma lipid profile, but its role on inflammation and insulin resistance is not fully elucidated yet. We evaluated the effect of sunflower oil supplementation on inflammatory state and insulin resistance condition in HFD-induced obese mice. C57BL/6 male mice (8 weeks) were divided in four groups: (a) control diet (CD), (b) HFD, (c) CD supplemented with n-6 (CD + n-6), and (d) HFD supplemented with n-6 (HFD + n-6). CD + n-6 and HFD + n-6 were supplemented with sunflower oil by oral gavage at 2 g/Kg of body weight, three times per week. CD and HFD were supplemented with water instead at the same dose. HFD induced whole and muscle-specific insulin resistance associated with increased inflammatory markers in insulin-sensitive tissues and macrophage cells. Sunflower oil supplementation was not efficient in preventing or reducing these parameters. In addition, the supplementation increased pro-inflammatory cytokine production by macrophages and tissues. Lipid profile, on the other hand, was improved with the sunflower oil supplementation in animals fed HFD. In conclusion, sunflower oil supplementation improves lipid profile, but it does not prevent or attenuate insulin resistance and inflammation induced by HFD in C57BL/6 mice.
authors.affiliation: Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, Avenu Professor Lineu Prestes, Butantã, 1524 São Paulo, SP, Brazil.
authors.forename: Laureane Nunes
authors.initials: LN
authors.lastname: Masi
authors.forename: Amanda Roque
authors.initials: AR
authors.lastname: Martins
authors.forename: José César
authors.initials: JC
authors.lastname: Rosa Neto
authors.forename: Cátia Lira
authors.initials: CL
authors.lastname: do Amaral
authors.forename: Amanda Rabello
authors.initials: AR
authors.lastname: Crisma
authors.forename: Marco Aurélio Ramirez
authors.initials: MA
authors.lastname: Vinolo
authors.forename: Edson Alves
authors.initials: EA
authors.lastname: de Lima Júnior
authors.forename: Sandro Massao
authors.initials: SM
authors.lastname: Hirabara
authors.forename: Rui
authors.initials: R
authors.lastname: Curi
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000009:adverse effects*; D019587:Dietary Supplements; D007249:Inflammation / Q000139:chemically induced* / Q000503:physiopathology; D007333:Insulin Resistance; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology*; D010938:Plant Oils / Q000008:administration & dosage* / Q000009:adverse effects*; D000074242:Sunflower Oil
chemical_list: D004041:Dietary Fats; D010938:Plant Oils; D000074242:Sunflower Oil