title: [Quantitative determination of sibricose A5 and sibricose A6 in Polygalae radix].
abstract: OBJECTIVE
  To establish the method for quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix by HPLC.
  
  
  METHODS
  The ultrasonic extracting method was applied in sample pre-treatment. The HPLC procedure was performed on the chromatographic column of Agela Promosil C18 (4.6 mm x 250 mm, 5 microm), the mobile phase was acetonitrile-0.1% phosphoric acid water solution (10:90). The detection wavelength was 330 nm and flow velocity was 1 mL x min(-1). The column temperature was 30 degrees C.
  
  
  RESULTS
  The method has good linearity in the ranges of 0.0087-0.0694 g x L(-1) (r = 0.9993) for sibricose A5, 0.0090-0.0723 g x L(-1) (r=0.9991) for sibricose A6. The average recoveries of sibricose A5 and sibricose A6 were 101.7%, 97.87%, with the RSD of 1.7%, 1.6%, respectively.
  
  
  CONCLUSIONS
  The method was simple, quick accurate and reliable. It is appropriate for the quantitative determination of sibricose A5 and sibricose A6 in Polygalae Radix.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. sunchangqingscq@126.com
authors.forename: Changqing
authors.initials: C
authors.lastname: Sun
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Peng
authors.forename: Nailiang
authors.initials: N
authors.lastname: Zhu
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Lili
authors.initials: L
authors.lastname: Kang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Cui
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Guo
authors.forename: Renbing
authors.initials: R
authors.lastname: Shi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031801:Polygala / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D004365:Drugs, Chinese Herbal; D011134:Polysaccharides