title: [Simultaneous inhibition of XIAP and survivin expression on EMT and invasion of human pancreatic cancer cells].
abstract: OBJECTIVE
  To investigate the simultaneous inhibition of X-linked inhibitor of apoptosis protein (XIAP) and survivin expression on epithelial-mesenchymal transition (EMT) and invasiion of pancreatic cancer cells Panc-1, and its mechanism.
  
  
  METHODS
  On the established human pancreatic cancer cells Panc-1-XS, the expression of XIAP and survivin was inhibited simultaneously. Cell invasion and migration were detected by Transwell chamber experiments and scratch test, and the expression of epithelial marker E-cadherin, mesenchymal markers Slug, phosphatase and tensin homolog deleted on chromosome ten (PTEN) and P-Akt protein was determined by Western blot.
  
  
  RESULTS
  Cell invasion and migration of Panc-1-XS cells decreased significantly, accompanied by significantly upregulated protein expression of E-cadherin, and significantly declined protein expression of the Slug, indicating increased mesenchymal-epithelial conversion (MET); and increased protein expression of PTEN, and declined protein expression of P-Akt.
  
  
  CONCLUSIONS
  Simultaneously inhibiting the expression of XIAP and survivin can partially reverse EMT phenotype of pancreatic cancer Panc-1 cells, which then significantly reduces the cell invasion and migration of Panc-1 cell lines. This process may be regulated by PTEN/PI3K/Akt signaling pathway.
authors.affiliation: Department of Gerneral Surgery, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zai
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yi
authors.forename: Yixiong
authors.initials: Y
authors.lastname: Li
authors.forename: Chun
authors.initials: C
authors.lastname: Jiang
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Lu
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000473:pathology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D000071250:Snail Family Transcription Factors; D000077022:Survivin; D014157:Transcription Factors / Q000378:metabolism; D051636:X-Linked Inhibitor of Apoptosis Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; C107489:BIRC5 protein, human; C513384:CDH1 protein, human; D015820:Cadherins; D051034:Inhibitor of Apoptosis Proteins; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; D000077022:Survivin; D014157:Transcription Factors; D051636:X-Linked Inhibitor of Apoptosis Protein; C496866:XIAP protein, human; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase