title: A universal nanoparticle cell secretion capture assay.
abstract: Secreted proteins play an important role in intercellular interactions, especially between cells of the immune system. Currently, there is no universal assay that allows a simple noninvasive identification and isolation of cells based on their secretion of various products. We have developed such a method. Our method is based on the targeting, to the cell surface, of heterofunctional nanoparticles coupled to a cell surface-specific antibody and to a secreted protein-specific antibody, which captures the secreted protein on the surface of the producing cell. Importantly, this method does not compromise cellviability and is compatible with further culture and expansion of the secreting cells.
authors.affiliation: Section on Intercellular Interactions, Program in Physical Biology, The Eunice Kennedy-Shriver National Institute of Health and Human Development, Bethesda, Maryland 20892, USA.
authors.forename: Wendy
authors.initials: W
authors.lastname: Fitzgerald
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Grivel
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D003102:Colloids; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000737:chemistry / Q000378:metabolism*; D007376:Interleukin-2 / Q000737:chemistry / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011485:Protein Binding; D045663:Quantum Dots
chemical_list: D055910:Antibodies, Immobilized; D003102:Colloids; C508594:IL2 protein, human; D007376:Interleukin-2; D007371:Interferon-gamma