title: Transfection of Plasmodium falciparum.
abstract: Genetic manipulation of Plasmodium falciparum remains very challenging, mainly due to the parasite genome's high A/T-richness and low transfection efficiency. This chapter includes methods for generating transient and stable transfections by electroporation, allelic replacement with tagged genes, gene deletion, and the analysis of all the above.
authors.affiliation: Research School of Biology, The Australian National University, Canberra, ACT, Australia. rug@wehi.edu.au
authors.forename: Melanie
authors.initials: M
authors.lastname: Rug
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Maier
mesh_terms: D018929:Cell Culture Techniques; D016054:DNA, Protozoan; D018274:Electroporation / Q000295:instrumentation / Q000379:methods; D004912:Erythrocytes / Q000469:parasitology; D023061:Gene Order; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods; D010957:Plasmids / Q000235:genetics; D010963:Plasmodium falciparum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
chemical_list: D016054:DNA, Protozoan