title: T-RFLP for detection and identification of phytoplasmas in plants.
abstract: Conventionally, detection of phytoplasmas has been performed by PCR of the 16S rRNA gene, followed by either RFLP or DNA sequencing to determine the phytoplasma 16Sr group. This chapter demonstrates the technique of terminal restriction fragment length polymorphism (T-RFLP), a fingerprinting technique which combines both detection and identification in a single method, with the added benefit of inbuilt controls which removes the risk of false negative results and in addition highlights potential false positive results.
authors.affiliation: The Food and Environment Research Agency, Sand Hutton, York, UK. jennifer.hodgetts@fera.gsi.gov.uk
authors.forename: Jennifer
authors.initials: J
authors.lastname: Hodgetts
authors.forename: Matt
authors.initials: M
authors.lastname: Dickinson
mesh_terms: D004269:DNA, Bacterial; D018744:DNA, Plant; D045582:Phytoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000382:microbiology; D010944:Plants / Q000235:genetics / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S