title: Real-time PCR for universal phytoplasma detection and quantification.
abstract: Currently, the most efficient detection and precise quantification of phytoplasmas is by real-time PCR. Compared to nested PCR, this method is less sensitive to contamination and is less work intensive. Therefore, a universal real-time PCR method will be valuable in screening programs and in other setups in which large numbers of samples are tested.
authors.affiliation: Center for Advanced Bioimaging, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Nynne Meyn
authors.initials: NM
authors.lastname: Christensen
authors.forename: Henriette
authors.initials: H
authors.lastname: Nyskjold
authors.forename: Mogens
authors.initials: M
authors.lastname: Nicolaisen
mesh_terms: D019295:Computational Biology / Q000379:methods; D004269:DNA, Bacterial; D018744:DNA, Plant; D045582:Phytoplasma / Q000235:genetics / Q000302:isolation & purification*; D010944:Plants / Q000235:genetics / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant