title: Characterization of insulin-producing cells derived from PDX-1-transfected             neural stem cells.
abstract: Islet cell transplantation is a promising treatment strategy for type-1             diabetes. However, functional islet cells are hard to obtain for transplantation             and are in short supply. Directing the differentiation of stem cells into insulin‑producing             cells, which serve as islet cells, would overcome this shortage. Bone marrow contains             hematopoietic stem cells and mesenchymal stem cells. The present study used bone             marrow cells isolated from rats and neural stem cells (NSCs) that were derived             from bone marrow cells in culture. Strong nestin staining was detected in NSCs,             but not in bone marrow stromal cells (BMSCs). In vitro transfection of the pancreatic             duodenal homeobox-1 (PDX-1) gene into NSCs generated insulin‑producing cells.             Reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent             assay (ELISA) analysis confirmed that PDX-1-transfected NSCs expressed insulin             mRNA and released insulin protein. However, insulin release from PDX-1-transfected             NSCs did not respond to the challenge of glucose and glucagon-like peptide-1.             These results support the use of bone marrow-derived NSCs as a renewable source             of insulin-producing cells for autologous transplantation to treat type-1 diabetes.
authors.affiliation: Department of Endocrinology, Shenzhen Longgang Central Hospital,                     Shenzhen 518000, P.R. China.
authors.forename: Hailan
authors.initials: H
authors.lastname: Wang
authors.forename: Zesheng
authors.initials: Z
authors.lastname: Jiang
authors.forename: Aihui
authors.initials: A
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D052216:Glucagon-Like Peptide 1 / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D064231:Nestin / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D018398:Homeodomain Proteins; D007328:Insulin; D064231:Nestin; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C485184:enhanced green fluorescent protein; C084010:pancreatic and duodenal homeobox 1 protein; D049452:Green Fluorescent Proteins; D052216:Glucagon-Like Peptide 1; D005947:Glucose