title: Preparation of a stationary phase with quaternary ammonium embedded group for selective separation of alkaloids based on ion-exclusion interaction.
abstract: A stationary phase (named QA C10) with quaternary ammonium embedded between a propyl and a decyl chain was synthesized by immobilization of N,N-dimethyldecylamine on chloropropyl-silica surface. A set of representative neutral, basic, and acidic compounds was employed to evaluate its chromatographic properties. The results illustrated that QA C10 was a mixed-mode stationary phase possessing both hydrophobic and ionic characteristics. The QA C10 stationary phase was further used for selective separation of alkaloids from Cortex phellodendri. Under acidic condition, alkaloids could be eluted in first 8 min, while other neutral and acidic fractions were retained better on QA C10 column. Then, obtained alkaloid fraction was analyzed by LC-MS/MS and 22 alkaloids were identified. Our study confirmed the advantages and application potential of the QA C10 stationary phase for alkaloids separation.
authors.affiliation: Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
authors.forename: Qiaoxia
authors.initials: Q
authors.lastname: Liu
authors.forename: Tu
authors.initials: T
authors.lastname: Liang
authors.forename: Kuiyong
authors.initials: K
authors.lastname: Li
authors.forename: Yanxiong
authors.initials: Y
authors.lastname: Ke
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D012117:Resins, Synthetic / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D000470:Alkaloids; D000644:Quaternary Ammonium Compounds; D012117:Resins, Synthetic