title: [A novel endogeneous inhibitor from hepatopancreas of Kamchatka crab (Paralithodes camtschaticus)].
abstract: A novel endogeneous inhibitor from hepatopancreas of Kamchatka crab (Paralithosed camtschaticus) was isolatyed. The inhibitor was purifeid through fractional affinity chromatography on gramicidin-diasorb followed by gel-filtration at Sephadex G-100. The inhibitor PC is a protein (M, 66 kDa) and active against serine collagenolytic protease PC at temperature optimum 15-20 degrees C, stable at 4-40 degrees C and was completely inactivated after heating to 50 degrees C and higher. 0.9-20% NaCl is necessary for its inhibitor activity. The inhibitor was found to slow down cell spreading in vitro in cell type-dependent manner. Fibroblasts are most prone to inhibitory effect, epithelial tumor derived cells show medium susceptibility, while fibrosarcoma cells were not affected.
authors.forename: S A
authors.initials: SA
authors.lastname: Kuznetsov
authors.forename: S A
authors.initials: SA
authors.lastname: Golyshev
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Rudenskaia
authors.forename: V A
authors.initials: VA
authors.lastname: Isaev
authors.forename: I P
authors.initials: IP
authors.lastname: Kuranova
authors.forename: G N
authors.initials: GN
authors.lastname: Rudenskaia
mesh_terms: D000818:Animals; D033381:Anomura / Q000737:chemistry*; D045744:Cell Line, Tumor; D002846:Chromatography, Affinity; D005347:Fibroblasts / Q000187:drug effects; D043143:Hepatopancreas / Q000737:chemistry*; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D012697:Serine Endopeptidases / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D015842:Serine Proteinase Inhibitors; D012697:Serine Endopeptidases