title: Identification of novel small-molecule inhibitors of glioblastoma cell growth and invasion by high-throughput screening.
abstract: Glioblastoma multiforme (GBM) is the most common and lethal type of primary brain tumor with a very poor prognosis. Current therapies for GBM remain palliative and advances made in decades have resulted in only a slight improvement in treatment outcome. Exploring new therapeutic agents for GBM treatment, therefore, is of prime importance. In the present study, we performed a high-throughput screening for GBM cell growth and invasion, with an attempt to identify novel potential anti-GBM agents. An annotated compound library (LOPAC1280) of 1,280 pharmacologically active compounds was screened and ten compounds were validated and identified as inhibitors of GBM cell growth and invasion. Four of them, i.e., 6-nitroso-1,2-benzopyrone, S-(p-azidophenacyl) glutathione, phenoxybenzamine hydrochloride, and SCH-28080 have not been implicated in GBM cell growth and invasion previously, suggesting that they may serve as novel potential therapeutic agents for GBM treatment. In conclusion, novel inhibitors of GBM cell growth and invasion were identified in the present study, which provides a basis for the development of therapies for GBM, and may shed light on the molecular mechanisms underlying GBM cell behavior.
authors.affiliation: Key Laboratory of the Ministry of Education for Experimental Teratology, Department of Histology and Embryology, School of Medicine, Shandong University, Ji'nan, Shandong, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Kemi
authors.initials: K
authors.lastname: Cui
authors.forename: Linli
authors.initials: L
authors.lastname: Yao
authors.forename: Min
authors.initials: M
authors.lastname: Ren
authors.forename: Aijun
authors.initials: A
authors.lastname: Hao
authors.forename: Patrick
authors.initials: P
authors.lastname: Smollen
authors.forename: Fang
authors.initials: F
authors.lastname: Nie
authors.forename: Guangxu
authors.initials: G
authors.lastname: Jin
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Stephen T C
authors.initials: ST
authors.lastname: Wong
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation; D004305:Dose-Response Relationship, Drug; D005909:Glioblastoma / Q000188:drug therapy* / Q000473:pathology*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D009361:Neoplasm Invasiveness; D015203:Reproducibility of Results; D054852:Small Molecule Libraries / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use*
chemical_list: D000970:Antineoplastic Agents; D054852:Small Molecule Libraries