title: Differential expression of cell surface markers in response to 2,4-dinitrofluorobenzene in RAW 264.7 and primary immune cells.
abstract: We evaluated the expression of the costimulatory molecules CD80 and CD83 and major histocompatibility (MHC) class II induced by 2,4-dinitrofluorobenzene (DNFB) in the RAW 264.7 macrophage cell line. In contrast to the previously reported effect of DNFB on dendritic cells, CD86 expression did not change. Furthermore, we observed that the CD83 expression level transiently increased and then decreased. Induction of CD80 and MHC class II molecule expression and a decrease in CD83 expression by DNFB in vitro were also confirmed in splenocytes of BALB/c and NC/Nga mice. However, DNFB did not influence CD83 expression in peritoneal CD11b(+) cells from BALB/c or NC/Nga mice. Detailed in vivo experiments and further studies on the possible contribution of CD11b(+) cells to induce atopic dermatitis (AD) would be helpful to attain a better understanding of AD pathogenesis.
authors.affiliation: Department of Microbiology, College of Medicine, Hallym University, Chuncheon 200-702, Korea.
authors.forename: Dongbum
authors.initials: D
authors.lastname: Kim
authors.forename: Min Chul
authors.initials: MC
authors.lastname: Park
authors.forename: Byoung Kwon
authors.initials: BK
authors.lastname: Park
authors.forename: Sanghoon
authors.initials: S
authors.lastname: Kwon
authors.forename: Joon-Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: Hyun-Jong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Jinseu
authors.initials: J
authors.lastname: Park
authors.forename: Younghe
authors.initials: Y
authors.lastname: Lee
authors.forename: Hyung-Joo
authors.initials: HJ
authors.lastname: Kwon
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism*; D018122:B7-1 Antigen / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D039481:CD11b Antigen / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000378:metabolism / Q000473:pathology; D004139:Dinitrofluorobenzene / Q000633:toxicity*; D020869:Gene Expression Profiling; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D018122:B7-1 Antigen; D015415:Biomarkers; D039481:CD11b Antigen; D004139:Dinitrofluorobenzene; D000949:Histocompatibility Antigens Class II; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen