title: Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system.
abstract: Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray® (FA; Idaho Technology, Salt Lake City, UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture.
authors.affiliation: Department of Pediatrics, University of Utah, Salt Lake City, UT, USA. anne.blaschke@hsc.utah.edu
authors.forename: Anne J
authors.initials: AJ
authors.lastname: Blaschke
authors.forename: Caroline
authors.initials: C
authors.lastname: Heyrend
authors.forename: Carrie L
authors.initials: CL
authors.lastname: Byington
authors.forename: Mark A
authors.initials: MA
authors.lastname: Fisher
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Barker
authors.forename: Nicholas F
authors.initials: NF
authors.lastname: Garrone
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Thatcher
authors.forename: Andrew T
authors.initials: AT
authors.lastname: Pavia
authors.forename: Trenda
authors.initials: T
authors.lastname: Barney
authors.forename: Garrison D
authors.initials: GD
authors.lastname: Alger
authors.forename: Judy A
authors.initials: JA
authors.lastname: Daly
authors.forename: Kirk M
authors.initials: KM
authors.lastname: Ririe
authors.forename: Irene
authors.initials: I
authors.lastname: Ota
authors.forename: Mark A
authors.initials: MA
authors.lastname: Poritz
mesh_terms: D000328:Adult; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*