title: Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
abstract: In tonsils, CD138(+) plasma cells (PCs) are surrounded by CD163(+) resident macrophages (Ms). We show here that human Ms (isolated from tonsils or generated from monocytes in vitro) drive activated B cells to differentiate into CD138(+)CD38(++) PCs through secreted CXCL10/IP-10 and VCAM-1 contact. IP-10 production by Ms is induced by B cell-derived IL-6 and depends on STAT3 phosphorylation. Furthermore, IP-10 amplifies the production of IL-6 by B cells, which sustains the STAT3 signals that lead to PC differentiation. IP-10-deficient mice challenged with NP-Ficoll show a decreased frequency of NP-specific PCs and lower titers of antibodies. Thus, our results reveal a novel dialog between Ms and B cells, in which IP-10 acts as a PC differentiation factor.
authors.affiliation: Baylor Institute for Immunology Research, Dallas, TX 75204, USA. wei.xu.wx6@roche.com
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: HyeMee
authors.initials: H
authors.lastname: Joo
authors.forename: Sandra
authors.initials: S
authors.lastname: Clayton
authors.forename: Melissa
authors.initials: M
authors.lastname: Dullaers
authors.forename: Marie-Cecile
authors.initials: MC
authors.lastname: Herve
authors.forename: Derek
authors.initials: D
authors.lastname: Blankenship
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: De La Morena
authors.forename: Robert
authors.initials: R
authors.lastname: Balderas
authors.forename: Capucine
authors.initials: C
authors.lastname: Picard
authors.forename: Jean-Laurent
authors.initials: JL
authors.lastname: Casanova
authors.forename: Virginia
authors.initials: V
authors.lastname: Pascual
authors.forename: SangKon
authors.initials: S
authors.lastname: Oh
authors.forename: Jacques
authors.initials: J
authors.lastname: Banchereau
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002450:Cell Communication; D002454:Cell Differentiation / Q000276:immunology*; D054357:Chemokine CXCL10 / Q000096:biosynthesis / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D017578:Immunoglobulin Class Switching / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007156:Immunologic Memory; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D014066:Palatine Tonsil / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D010950:Plasma Cells / Q000166:cytology* / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D000098902:CD163 Antigen
chemical_list: D000906:Antibodies; D000941:Antigens; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D054357:Chemokine CXCL10; D007074:Immunoglobulin G; D015850:Interleukin-6; D011956:Receptors, Cell Surface; D050796:STAT3 Transcription Factor; D019010:Vascular Cell Adhesion Molecule-1