title: [Mapping of the 3' end for artificial human microRNA].
abstract: The 3' end was exactly mapped for has-mir-30a-like artificial human microRNAs that specifically recognize the mRNA of the aml1/eto fusion oncogene. The results indicated that the intracellular microRNA pool was heterogeneous in linear size relative to the 3' end, which is necessary to consider in designing and using artificial microRNAs specific for mRNAs of other genes.
authors.forename: V V
authors.initials: VV
authors.lastname: Grinev
authors.forename: D V
authors.initials: DV
authors.lastname: Posrednik
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000138:chemical synthesis* / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D016372:RNA, Antisense / Q000138:chemical synthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D000075142:RUNX1 Translocation Partner 1 Protein
chemical_list: D020413:3' Untranslated Regions; C107844:AML1-ETO fusion protein, human; D050676:Core Binding Factor Alpha 2 Subunit; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D015514:Oncogene Proteins, Fusion; D016372:RNA, Antisense; D012333:RNA, Messenger; D000075142:RUNX1 Translocation Partner 1 Protein