title: [Human enterovirus 71 (EV71) resisted type I interferon induced antiviral effect].
abstract: OBJECTIVE
  To investigate human enterovirus 71 (EV71) resistance to type I interferon induced antiviral effect.
  
  
  METHODS
  After type I interferons (alpha, beta) were incubated with HeLa cells, recombinant type I herpes simple virus (HSV-1) with green fluorescent protein (GFP) was inoculated onto the HeLa cells. HSV-1 proliferation was observed by GFP expression and PCR. After EV71 was inoculated onto HeLa cells incubated with the same quantity of interferon, proliferation of EV71 were detected by RT-PCR of 2A gene.
  
  
  RESULTS
  Recombinant HSV-1 GFP expression and viral DNA replication obviously decreased in HeLa cells incubated with type I interferon (alpha, beta). However, EV71 effectively proliferated in the interferon irritated HeLa cell by RT-PCR.
  
  
  CONCLUSIONS
  HeLa cell irritated by type I interferon (alpha, beta) produced antiviral substance that inhibits HSV-1 proliferation. EV71 resisted the antiviral substance induced by type I interferon and could significantly replicate in the HeLa cells.
authors.affiliation: Medical School, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cui
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Song
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Xing
authors.initials: X
authors.lastname: Gan
authors.forename: Gong-Qi
authors.initials: GQ
authors.lastname: Li
authors.forename: Ke-Xia
authors.initials: KX
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Han
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D024882:Drug Resistance, Viral; D029821:Enterovirus A, Human / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D007370:Interferon Type I