title: Activation of human NK cells by Plasmodium-infected red blood cells.
abstract: This chapter describes a protocol to assess activation of human NK cells following in vitro stimulation with malaria-infected red blood cells. Activation is assessed by flow cytometry, staining for cell surface expression of CD69 and accumulation of intracellular IFN-γ. Procedures are described for in vitro propagation and purification of Plasmodium falciparum parasites, separation of peripheral blood mononuclear cells from heparinized blood by density centrifugation, in vitro culture of PBMC and for staining and analysis of PBMC by flow cytometry. Some examples of typical FACS plots are shown.
authors.affiliation: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK.
authors.forename: Amir
authors.initials: A
authors.lastname: Horowitz
authors.forename: Eleanor M
authors.initials: EM
authors.lastname: Riley
mesh_terms: D018929:Cell Culture Techniques; D002469:Cell Separation / Q000379:methods; D004912:Erythrocytes / Q000276:immunology* / Q000469:parasitology*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D007694:Killer Cells, Natural / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D010961:Plasmodium / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology