title: Pharmacokinetics and risk evaluation of DNA vaccine against Schistosoma japonicum.
abstract: DNA plasmid immunization is a novel approach of preventive and therapeutic vaccine. More than 100 DNA vaccines have been on preclinical or clinical phase trials, and four kinds of DNA vaccines for livestock have been approved by USDA, CFIA, and APVMA. Schistosomiasis is a worldwide parasitic disease, and vaccine immunization is supposed to be a promising approach to control the health crisis. On the basis of former preclinical studies, we further focused on the pharmacokinetics and risk evaluation of DNA vaccine in vivo. In the present study, enhanced green fluorescent protein (EGFP) report gene was fused with Schistosoma japonicum 23 kDa transmembrane protein antigen gene (Sj23) and constructed into DNA vaccine pVIVO2-Sj23.EGFP. After intramuscularly injecting 100 μg of purified DNA vaccine plasmid to immunizate BALB/c mice, we studied the tissue distribution of DNA plasmid and expressed Sj23.EGFP antigen, the persistence time of elicited antibodies, and the risk of DNA vaccine transferred into intestinal microorganisms. The results showed that DNA vaccine plasmid could be distributed into all tissues of the body after injection; however, only few organs including the injected muscle were detected DNA vaccine at postimmunization until the 100 days by PCR technology; the detection of green fluorescence protein displayed that DNA vaccine could be expressed in almost every tissue and organs; the ELISA assay indicated the immune antibody against Sj23 could persist over 70 days; and the DNA vaccine transferring intestinal flora results was negative. The results indicated that the DNA vaccine has systemic protection and long-lasting effectivity and is safe to intestinal flora.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, China. haifeng_hust@163.com
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Ming-Bo
authors.initials: MB
authors.lastname: Lu
authors.forename: Long-Jiang
authors.initials: LJ
authors.lastname: Yu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005453:Fluorescence; D041981:Gastrointestinal Tract / Q000382:microbiology; D022761:Gene Transfer, Horizontal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D007273:Injections, Intramuscular; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000008:administration & dosage / Q000009:adverse effects / Q000493:pharmacokinetics; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012549:Schistosoma japonicum / Q000235:genetics / Q000276:immunology*; D013997:Time Factors; D014018:Tissue Distribution; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology / Q000493:pharmacokinetics*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D019444:Vaccines, DNA; C485184:enhanced green fluorescent protein; C072633:Sj23 antigen, Schistosoma japonicum; D049452:Green Fluorescent Proteins