title: Calcium tips the balance: a microtubule plus end to lattice binding switch operates in the carboxyl terminus of BPAG1n4.
abstract: Microtubules (MTs) are integral to numerous cellular functions, such as cell adhesion, differentiation and intracellular transport. Their dynamics are largely controlled by diverse MT-interacting proteins, but the signalling mechanisms that regulate these interactions remain elusive. In this report, we identify a rapid, calcium-regulated switch between MT plus end interaction and lattice binding within the carboxyl terminus of BPAG1n4. This switch is EF-hand dependent, and mutations of the EF-hands abolish this dynamic behaviour. Our study thus uncovers a new, calcium-dependent regulatory mechanism for a spectraplakin, BPAG1n4, at the MT plus end.
authors.affiliation: Department of Neurology and Neurological Sciences, Stanford University School of Medicine, MSLS Building, Room P259, 1201 Welch Road, Stanford, California 94305, USA.
authors.forename: Mridu
authors.initials: M
authors.lastname: Kapur
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Michael T
authors.initials: MT
authors.lastname: Maloney
authors.forename: Ivan
authors.initials: I
authors.lastname: Millan
authors.forename: Victor F
authors.initials: VF
authors.lastname: Lundin
authors.forename: Thuy-An
authors.initials: TA
authors.lastname: Tran
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000818:Animals; D019556:COS Cells; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000071821:Dystonin; D020832:EF Hand Motifs; D057809:HEK293 Cells; D006801:Humans; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism*; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C486268:DST protein, human; D000071821:Dystonin; D009419:Nerve Tissue Proteins; D002118:Calcium