title: Nanometer-sized alumina packed microcolumn solid-phase extraction combined with field-amplified sample stacking-capillary electrophoresis for the speciation analysis of inorganic selenium in environmental water samples.
abstract: In this paper, a new method of nanometer-sized alumina packed microcolumn SPE combined with field-amplified sample stacking (FASS)-CE-UV detection was developed for the speciation analysis of inorganic selenium in environmental water samples. Self-synthesized nanometer-sized alumina was packed in a microcolumn as the SPE adsorbent to retain Se(IV) and Se(VI) simultaneously at pH 6 and the retained inorganic selenium was eluted by concentrated ammonia. The eluent was used for FASS-CE-UV analysis after NH₃ evaporation. The factors affecting the preconcentration of both Se(IV) and Se(VI) by SPE and FASS were studied and the optimal CE separation conditions for Se(IV) and Se(VI) were obtained. Under the optimal conditions, the LODs of 57 ng L⁻¹ (Se(IV)) and 71 ng L⁻¹ (Se(VI)) were obtained, respectively. The developed method was validated by the analysis of a certified reference material of GBW(E)080395 environmental water and the determined value was in a good agreement with the certified value. It was also successfully applied to the speciation analysis of inorganic selenium in environmental water samples, including Yangtze River water, spring water, and tap water.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine-Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, P. R. China.
authors.forename: Jiankuan
authors.initials: J
authors.lastname: Duan
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
authors.forename: Man
authors.initials: M
authors.lastname: He
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D060766:Drinking Water / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results; D045483:Rivers / Q000737:chemistry; D012643:Selenium / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000295:instrumentation* / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D060766:Drinking Water; D014874:Water Pollutants, Chemical; D012643:Selenium; D000537:Aluminum Oxide