title: Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source.
abstract: Efficient biosynthesis of succinate from a renewable biomass resource by engineered Escherichia coli is reported in this paper. Fermentation of sugarcane bagasse hydrolysate by engineered E. coli BA204, a pflB, ldhA, and ppc deletion strain overexpressing the ATP-forming phosphoenolpyruvate carboxykinase from Bacillus subtilis 168, produced a final succinate concentration of 15.85 g L(-1) with a high yield of 0.89 g L(-1) total sugar under anaerobic conditions. During dual-phase fermentations, initial aerobic growth facilitated subsequent anaerobic succinate production, with a final succinate concentration of 18.88 g L(-1) and a yield of 0.96 g g(-1) total sugar after 24 h of anaerobic fermentation. The high succinate yield from sugarcane bagasse hydrolysate demonstrated a great potential application of renewable biomass as a feedstock for the economical production of succinate using metabolically engineered E. coli.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, China.
authors.forename: Rongming
authors.initials: R
authors.lastname: Liu
authors.forename: Liya
authors.initials: L
authors.lastname: Liang
authors.forename: Weijia
authors.initials: W
authors.lastname: Cao
authors.forename: Mingke
authors.initials: M
authors.lastname: Wu
authors.forename: Kequan
authors.initials: K
authors.lastname: Chen
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Ma
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Pingkai
authors.initials: P
authors.lastname: Ouyang
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D002241:Carbohydrates / Q000494:pharmacology; D002244:Carbon / Q000494:pharmacology*; D002482:Cellulose / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D060847:Metabolic Engineering / Q000379:methods*; D031786:Saccharum / Q000737:chemistry*; D019802:Succinic Acid / Q000378:metabolism*
chemical_list: D002241:Carbohydrates; D002244:Carbon; D002482:Cellulose; C027433:bagasse; D019802:Succinic Acid