title: Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films.
abstract: We developed a rapid and simple method for evaluating the degradation of solid biodegradable plastics (BPs). Dye-containing BP films were used as substrates and the release of dye caused by the degradation of BPs was confirmed by a color change in the enzyme solution after a reaction time of 24 h.
authors.affiliation: National Institute for Agro-environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki 305-8604, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Shinozaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakajima-Kambe
authors.forename: Hiroko K
authors.initials: HK
authors.lastname: Kitamoto
mesh_terms: D063566:Biodegradable Plastics / Q000032:analysis* / Q000378:metabolism*; D003116:Color; D003124:Colorimetry / Q000379:methods*; D004396:Coloring Agents / Q000032:analysis; D057075:Enzyme Assays; D004798:Enzymes / Q000378:metabolism*; D013997:Time Factors
chemical_list: D063566:Biodegradable Plastics; D004396:Coloring Agents; D004798:Enzymes