title: Delayed-type hypersensitivity determination.
abstract: Delayed-type hypersensitivity responses in the skin (in the case of mice, in the foot pad) is used to assess cell-mediated immunity (CMI) in vivo. In the case of CMI to Helicobacter infection, the mice are given an injection of cultured Helicobacter organisms into the hind footpad, and induration is measured at the site of inoculation 24 h after inoculation. Here we describe the methods for assessing delayed-type hypersensitivity in the mouse.
authors.affiliation: Unit for Laboratory Animal Medicine, Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Gray
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Eaton
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D016480:Helicobacter pylori / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000382:microbiology*; D007111:Immunity, Cellular; D051379:Mice