title: Osmotic Pressure Induced Coupling between Cooperativity and Stability of a Helix-Coil Transition.
abstract: Most helix-coil transition theories can be characterized by three parameters: energetic, describing the (free) energy cost of forming a helical state in one repeating unit; entropic, accounting for the decrease of entropy due to formation of the helical state; and geometric, indicating how many repeating units are affected by the formation of one helical state. Depending on their effect on the helix-coil transition, solvents or cosolutes can be classified with respect to their action on these parameters. Solvent interactions that alter the entropic cost of helix formation by their osmotic action can affect both the stability (transition temperature) and the cooperativity (transition interval) of the helix-coil transition. Consistent inclusion of osmotic pressure effects in a description of helix-coil transition, for poly(L-glutamic acid) in solution with polyethylene glycol, can offer an explanation of the experimentally observed linear dependence of transition temperature on osmotic pressure as well as the concurrent changes in the cooperativity of the transition.
authors.affiliation: Department of Theoretical Physics, J. Stefan Institute, Ljubljana, Slovenia, EU. abadasyan@gmail.com
authors.forename: Artem
authors.initials: A
authors.lastname: Badasyan
authors.forename: Shushanik
authors.initials: S
authors.lastname: Tonoyan
authors.forename: Achille
authors.initials: A
authors.lastname: Giacometti
authors.forename: Rudolf
authors.initials: R
authors.lastname: Podgornik
authors.forename: V Adrian
authors.initials: VA
authors.lastname: Parsegian
authors.forename: Yevgeni
authors.initials: Y
authors.lastname: Mamasakhlisov
authors.forename: Vladimir
authors.initials: V
authors.lastname: Morozov
mesh_terms: D019277:Entropy; D008956:Models, Chemical; D008958:Models, Molecular; D009995:Osmosis; D011092:Polyethylene Glycols / Q000737:chemistry; D011099:Polyglutamic Acid / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D011099:Polyglutamic Acid; D011092:Polyethylene Glycols