title: Comparison of dkgB-linked intergenic sequence ribotyping to DNA microarray hybridization for assigning serotype to Salmonella enterica.
abstract: Two DNA-based methods were compared for the ability to assign serotype to 139 isolates of Salmonella enterica ssp. I. Intergenic sequence ribotyping (ISR) evaluated single nucleotide polymorphisms occurring in a 5S ribosomal gene region and flanking sequences bordering the gene dkgB. A DNA microarray hybridization method that assessed the presence and the absence of sets of genes was the second method. Serotype was assigned for 128 (92.1%) of submissions by the two DNA methods. ISR detected mixtures of serotypes within single colonies and it cost substantially less than Kauffmann-White serotyping and DNA microarray hybridization. Decreasing the cost of serotyping S. enterica while maintaining reliability may encourage routine testing and research.
authors.affiliation: Agricultural Research Service, U.S. Department of Agriculture, Athens, GA 30605, USA. jean.guard@ars.usda.gov
authors.forename: Jean
authors.initials: J
authors.lastname: Guard
authors.forename: Roxana
authors.initials: R
authors.lastname: Sanchez-Ingunza
authors.forename: Cesar
authors.initials: C
authors.lastname: Morales
authors.forename: Tod
authors.initials: T
authors.lastname: Stewart
authors.forename: Karen
authors.initials: K
authors.lastname: Liljebjelke
authors.forename: Joann
authors.initials: J
authors.lastname: Van Kessel
authors.forename: Kim
authors.initials: K
authors.lastname: Ingram
authors.forename: Deana
authors.initials: D
authors.lastname: Jones
authors.forename: Charlene
authors.initials: C
authors.lastname: Jackson
authors.forename: Paula
authors.initials: P
authors.lastname: Fedorka-Cray
authors.forename: Jonathan
authors.initials: J
authors.lastname: Frye
authors.forename: Richard
authors.initials: R
authors.lastname: Gast
authors.forename: Arthur
authors.initials: A
authors.lastname: Hinton
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic; D006801:Humans; D046228:Microarray Analysis / Q000191:economics / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D021521:Ribotyping / Q000191:economics / Q000379:methods*; D012480:Salmonella Infections / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics*; D012703:Serotyping / Q000191:economics / Q000379:methods; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic; D013401:Sugar Alcohol Dehydrogenases