title: Shotgun cholanomics of ileal fluid.
abstract: In this study we have developed a rapid method for the shotgun analysis of bile acids in intestinal fluid. The method is semi-quantitative, and requires little sample preparation. Bile salts might contribute to the pathogenesis of Crohn's disease. In a pilot study we demonstrate the method by analysing the bile acid content of ileal fluid from seven Crohn's disease patients and three healthy controls. The dominant bile acids observed were di and/or trihydroxycholanoates, di- and/or trihydroxycholanoylglycines, di- and/or tri-hydroxycholanoyltaurines, monosulphated dihydroxycholanoates and monosulphated dihydroxycholanoylglycine. The method can be similarly applied to samples derived from other parts of the intestine.
authors.affiliation: Clinical Laboratory, The Second Hospital of Shandong University, Jinan, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Chen
authors.forename: Michael
authors.initials: M
authors.lastname: Ogundare
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Williams
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Wang
authors.forename: Gavin W
authors.initials: GW
authors.lastname: Sewell
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Smith
authors.forename: Farooq Z
authors.initials: FZ
authors.lastname: Rahman
authors.forename: Nuala
authors.initials: N
authors.lastname: O'Shea
authors.forename: Anthony W
authors.initials: AW
authors.lastname: Segal
authors.forename: William J
authors.initials: WJ
authors.lastname: Griffiths
mesh_terms: D001647:Bile Acids and Salts / Q000378:metabolism*; D001826:Body Fluids / Q000378:metabolism*; D016022:Case-Control Studies; D003424:Crohn Disease / Q000378:metabolism; D006801:Humans; D007082:Ileum / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D001647:Bile Acids and Salts