title: Developmental analysis of liver metabolic proteins using mitochondrial antibody microarrays.
abstract: OBJECTIVE
  To investigate the abundance of metabolic proteins in adult and fetal human livers.
  
  
  METHODS
  Adult liver homogenate proteins, fetal liver homogenate proteins, adult liver mitochondrial proteins and fetal liver mitochondrial proteins were obtained from fetal or adult liver tissues and examined using the antibody microarrays containing 19 liver monoclonal mitochondrial antibodies. The protein expression abundances were compared among the 4 protein fractions and the pathways related to protein metabolisms were explored.
  
  
  RESULTS
  In adult liver mitochondria, aldehyde oxidase and carbonyl reductase were up-regulated by 2.6 and 1.7 folds, respectively, whereas corticosteroid 11-beta-dehydrogenase isozyme 1, epoxide hydrolase 1 and fibrinogen beta chain protein were down-regulated by 1.7, 1.9 and 2.2 folds, respectively, compared to those in fetal liver mitochondria. The abundance of epoxide hydrolase 1 and glutathione transferase omega-1 was significantly different between adult and fetal liver homogenate samples.
  
  
  CONCLUSIONS
  Our results demonstrate a clear difference in the expression profiles of metabolic proteins in the liver between adults and human fetuses to allow a better understanding of the occurrence and development of the metabolic proteins and the identification of markers of liver metabolism.
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yan
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
mesh_terms: D000328:Adult; D000906:Antibodies / Q000235:genetics / Q000378:metabolism; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000378:metabolism*; D040081:Protein Array Analysis
chemical_list: D000906:Antibodies; D024101:Mitochondrial Proteins