title: Immunogenicity of Cpn0425 and its localization in cells infected with             Chlamydophila pneumoniae.
abstract: The present study aimed to determine the intracellular localization of Cpn0425             in Chlamydophila pneumoniae-infected cells. The recombinant plasmid pGEX-6p/Cpn0425             was transformed into E.coli Bl21 cells to express the fusion protein. Following             purification with glutathione S-transferase (GST) resin chromatography, the Cpn0425             fusion protein was used to induce immunity in mice to develop monoclonal and polyclonal             antibodies, which were subsequently used to localize the endogenous Cpn0425 protein             by indirect immunofluorescence assay (IFA). ELISA was used to determine the immunogenicity             of the Cpn0425 plasmid protein by recognizing the pool sera of patients infected             with Chlamydia trachomatis and the pool sera of mice immunized with the Cpn0425             fusion protein. The Cpn0425 gene was expressed as the GST-Cpn0425 fusion protein             in E. coli and its antibody was prepared by immunizing mice with the fusion protein.             An anti-GST-Cpn0425 antibody was used to localize the protein in cells infected             with Chlamydophila pneumoniae AR-39 using an IFA. The anti-GST-CT058 antibody             detected an inclusion signal in the IFA. Cpn0425 protein strongly reacted with             antiserum. Although Cpn0425 protein is not a secreted protein, it has good immunogenicity.             Therefore, this protein may be useful for developing vaccines against Chlamydophila             pneumoniae infection.
authors.affiliation: Pathogenic Biology Institute, University of South China, Hengyang                     421001, P.R. China.
authors.forename: Liangzhuan
authors.initials: L
authors.lastname: Liu
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: You
authors.forename: Liesong
authors.initials: L
authors.lastname: Chen
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Zeng
authors.forename: Shuangyang
authors.initials: S
authors.lastname: Tang
authors.forename: Minjun
authors.initials: M
authors.lastname: Yu
authors.forename: Yimou
authors.initials: Y
authors.lastname: Wu
authors.forename: Xi
authors.initials: X
authors.lastname: Xhen
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D023521:Chlamydophila Infections / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016993:Chlamydophila pneumoniae / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D010957:Plasmids / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D011993:Recombinant Fusion Proteins