title: Monitoring of fluconazole in serum of patients undergoing hemodiafiltration by solid-phase extraction and high-performance liquid chromatography with ultraviolet detection.
abstract: A high-performance liquid chromatographic assay for monitoring the antifungal drug fluconazole in human serum was developed using a C18 reversed-phase column with isocratic elution. The method involved sample clean-up by solid-phase column extraction, and subsequent analysis required only 14 min per sample for separation and quantitation. The assay was precise, with intra- and inter-assay relative standard deviations of < or = 1.5% and < or = 3.1%. The minimum detectable concentration of fluconazole was 0.3 nmol/ml. This assay has the advantage of minimizing the risk of interference from co-administered drugs to critically ill patients undergoing hemodiafiltration.
authors.affiliation: Department of Analytical Chemistry of Medicines, Showa Pharmaceutical University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mizoguchi
authors.forename: K
authors.initials: K
authors.lastname: Hirata
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
authors.forename: T
authors.initials: T
authors.lastname: Chikuma
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000935:Antifungal Agents / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D016903:Drug Monitoring / Q000379:methods; D015725:Fluconazole / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D017583:Hemodiafiltration; D006801:Humans; D007275:Injections, Intravenous; D057230:Limit of Detection; D008297:Male; D015203:Reproducibility of Results; D052616:Solid Phase Extraction; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000935:Antifungal Agents; D015725:Fluconazole