title: Longevity-promoting effects of 4-hydroxy-E-globularinin in Caenorhabditis elegans.
abstract: In modern times, there has been a major increase in the use of plants or herbal constituents for the prevention of age-related disorders. 4-Hydroxy-E-globularinin (4-HEG) is an iridoid and a major component of Premna integrifolia. This investigation represents a breakthrough in geriatrics by showing the longevity-promoting activity of 4-HEG in the animal model Caenorhabditis elegans. 4-HEG (20μM) enhanced the mean life span of worms by over 18.8% under normal culture conditions and also enhanced their survival under oxidative stress. The longevity-promoting activity was associated with reduced reactive oxygen species (ROS) levels and fat accumulation in the worms. Gene-specific mutant studies verified the role of ROS detoxification pathways and simultaneous nuclear translocation of DAF-16 in the 4-HEG-mediated effects. Quantitative real-time PCR estimations and observations of transcriptional reporters indicated that 4-HEG was able to upregulate stress-inducible genes, viz., hsp-16.2 and sod-3. Thus, 4-HEG may serve as a lead compound of plant origin for the development of important nutraceuticals superseding the aging process.
authors.affiliation: Microbial Technology and Nematology, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
authors.forename: Virendra
authors.initials: V
authors.lastname: Shukla
authors.forename: Deepti
authors.initials: D
authors.lastname: Yadav
authors.forename: Suresh C
authors.initials: SC
authors.lastname: Phulara
authors.forename: M M
authors.initials: MM
authors.lastname: Gupta
authors.forename: Shilpi K
authors.initials: SK
authors.lastname: Saikia
authors.forename: Rakesh
authors.initials: R
authors.lastname: Pandey
mesh_terms: D000375:Aging / Q000187:drug effects; D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002633:Chemotaxis / Q000187:drug effects; D051858:Forkhead Transcription Factors; D005609:Free Radicals / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D017238:Genes, Helminth; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D039823:Iridoids / Q000737:chemistry / Q000494:pharmacology*; D008017:Life Expectancy; D008136:Longevity / Q000187:drug effects*; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects; D024301:Plant Bark / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: C581526:4-hydroxy-E-globularinin; D029742:Caenorhabditis elegans Proteins; D051858:Forkhead Transcription Factors; D005609:Free Radicals; D006360:Heat-Shock Proteins; D039823:Iridoids; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C108831:daf-16 protein, C elegans; C108431:hsp-16.2 protein, C elegans