title: Oxamflatin improves developmental competence of porcine somatic cell nuclear transfer embryos.
abstract: Abstract   Aberrant epigenetic nuclear reprogramming of somatic nuclei is a major cause of low success in cloning. It has been demonstrated that treatment of histone deacetylase inhibitors (HDACi) enhances developmental potential of somatic cell nuclear transfer (SCNT) embryos by alteration of epigenetic status. The aim of the present study was to investigate the effect of oxamflatin, a novel HDACi, on the developmental competence of porcine SCNT embryos. Treatment with 1 μM oxamflatin for 9 h after activation of SCNT embryos increased both in vitro and in vivo developmental competence. Treatment of SCNT embryos with 1 μM oxamflatin significantly increased blastocyst rate and total cell number in blastocysts (33.3±6.0 and 73.1±1.6, respectively) than that of controls (10.3±3.7 and 54.1±3.5, respectively) or scriptaid (16.4±4.6 and 64.4±2.1, respectively). Moreover, oxamflatin showed significant higher overall cloning efficiency from 0.9% to 3.2%, whereas scriptaid demonstrated 0% to 1.8%. In conclusion, these results indicate that oxamflatin treatment improves the developmental competence of porcine SCNT embryos.
authors.affiliation: Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Sol-Ji
authors.initials: SJ
authors.lastname: Park
authors.forename: Hee-Jung
authors.initials: HJ
authors.lastname: Park
authors.forename: Ok-Jae
authors.initials: OJ
authors.lastname: Koo
authors.forename: Woo-Jae
authors.initials: WJ
authors.lastname: Choi
authors.forename: Joon-Ho
authors.initials: JH
authors.lastname: Moon
authors.forename: Dae-Kee
authors.initials: DK
authors.lastname: Kwon
authors.forename: Jung-Taek
authors.initials: JT
authors.lastname: Kang
authors.forename: Sujin
authors.initials: S
authors.lastname: Kim
authors.forename: Ji-Yei
authors.initials: JY
authors.lastname: Choi
authors.forename: Goo
authors.initials: G
authors.lastname: Jang
authors.forename: Byeong-Chun
authors.initials: BC
authors.lastname: Lee
mesh_terms: D000818:Animals; D004624:Embryo Transfer; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology*; D006898:Hydroxylamines / Q000494:pharmacology; D053652:Nuclear Transfer Techniques; D010312:Parthenogenesis; D011247:Pregnancy; D011804:Quinolines / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D006898:Hydroxylamines; D011804:Quinolines; C100692:oxamflatin; C410733:scriptaid