title: [Method for discriminating key quality control indicators of concentrated solution before traditional Chinese medicine ethanol precipitation].
abstract: OBJECTIVE
  To suggest a method for discriminating quality control indicators of concentrated solution before traditional Chinese medicine ethanol precipitation.
  
  
  METHODS
  The second ethanol precipitation (SEP) of Guanxinning was taken as an example, with the concentrated supernatant of first ethanol precipitation (CSFEP) prepared with same dry matter content and the same amount of ethanol as raw materials, to conduct the ethanol precipitation test under the same conditions. The experimental data was analyzed by stepwise regression and partial least squares regression. Both of them selected the parameters which affect phenolic compounds retention ratios in SEP supernatant as the key indicators.
  
  
  RESULTS
  Phenolic compounds contents in SEP supernatant were mainly affected by the contents of danshensu, caffeic acid and salvianolic acid B in CSFEP.
  
  
  CONCLUSIONS
  The discrimination method can be used to discover key quality control indicators of concentrated solution before ethanol precipitation.
authors.affiliation: College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. 13805783212@139.com
authors.forename: Anyi
authors.initials: A
authors.lastname: Yan
authors.forename: Xingchu
authors.initials: X
authors.lastname: Gong
authors.forename: Haibin
authors.initials: H
authors.lastname: Qu
mesh_terms: D011232:Chemical Precipitation; D000431:Ethanol / Q000737:chemistry*; D008516:Medicine, Chinese Traditional / Q000592:standards*; D011786:Quality Control; D012996:Solutions
chemical_list: D012996:Solutions; D000431:Ethanol