title: Aminoacylase 3 binds to and cleaves the N-terminus of the hepatitis C virus core protein.
abstract: Aminoacylase 3 (AA3) mediates deacetylation of N-acetyl aromatic amino acids and mercapturic acids. Deacetylation of mercapturic acids of exo- and endobiotics are likely involved in their toxicity. AA3 is predominantly expressed in kidney, and to a lesser extent in liver, brain, and blood. AA3 has been recently reported to interact with the hepatitis C virus core protein (HCVCP) in the yeast two-hybrid system. Here we demonstrate that AA3 directly binds to HCVCP (K(d) ~10 μM) that may by implicated in HCV pathogenesis. AA3 also revealed a weak endopeptidase activity towards the N-terminus of HCVCP.
authors.affiliation: Division of Nephrology, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, CA 90095, USA. ktsirulnikov@mednet.ucla.edu
authors.forename: Kirill
authors.initials: K
authors.lastname: Tsirulnikov
authors.forename: Natalia
authors.initials: N
authors.lastname: Abuladze
authors.forename: Ritu
authors.initials: R
authors.lastname: Vahi
authors.forename: Huma
authors.initials: H
authors.lastname: Hasnain
authors.forename: Martin
authors.initials: M
authors.lastname: Phillips
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Ryan
authors.forename: Ivo
authors.initials: I
authors.lastname: Atanasov
authors.forename: Kym F
authors.initials: KF
authors.lastname: Faull
authors.forename: Ira
authors.initials: I
authors.lastname: Kurtz
authors.forename: Alexander
authors.initials: A
authors.lastname: Pushkin
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D000595:Amino Acid Sequence; D000818:Animals; D004926:Escherichia coli / Q000235:genetics; D016174:Hepacivirus / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D051379:Mice; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D011485:Protein Binding; D059748:Proteolysis; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D017386:Sequence Homology, Amino Acid; D012996:Solutions; D020349:Surface Plasmon Resonance; D020798:Two-Hybrid System Techniques; D014758:Viral Core Proteins / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure
chemical_list: D011993:Recombinant Fusion Proteins; D012996:Solutions; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus; D000581:Amidohydrolases; C483737:aminoacylase III, mouse