title: Sperm selection for ICSI using annexin V.
abstract: Annexin-V magnetic-activated cell sorting (MACS) is a new tool to optimize sperm selection in assisted reproduction. The technique is based on the binding of superparamagnetic Annexin-microbeads to externalized phosphatidylserine at the outer leaflet of the plasma membrane of sperm with activated apoptosis signaling or membrane damage. The combination of Annexin-V MACS and density gradient centrifugation was demonstrated to enhance clinical pregnancy rates in ICSI cycles. This chapter focuses on the practical details of Annexin-V MACS.
authors.affiliation: EAA Training Center, Klinik und Poliklinik für Dermatologie, Venerologie und Allergologie, Universitätsklinikum Leipzig AöR, Leipzig, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Grunewald
authors.forename: Uwe
authors.initials: U
authors.lastname: Paasch
mesh_terms: D017304:Annexin A5 / Q000378:metabolism*; D002499:Centrifugation, Density Gradient / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008297:Male; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*
chemical_list: D017304:Annexin A5