title: Capillary electrophoresis with field-amplified sample stacking for rapid and sensitive determination of sulfadiazine and sulfamethoxazole.
abstract: A new capillary electrophoresis method with field-amplified sample stacking (FASS) was developed for the analysis of sulfadiazine and sulfamethoxazole. After optimization of the separation and concentration conditions, the two compounds can be separated within 7 min and quantified with high sensitivity, with detection limits of 0.48 ng/mL for sulfadiazine and 0.76 ng/mL for sulfamethoxazole. This resulted in a 300-1500-fold improvement in concentration sensitivity relative to conventional capillary electrophoresis methods. The method was useful for qualitative and quantitative analysis of sulfadiazine and sulfamethoxazole in their preparations with recovery of 99.0%-102% for sulfadiazine and 99.5% - 99.7% for sulfamethoxazole.
authors.affiliation: Pharmacy College, Taishan Medicine College, Taian, P. R. China. liyuqin@tsmc.edu.cn
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Cui
authors.forename: Baoxiu
authors.initials: B
authors.lastname: Jia
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Caihong
authors.initials: C
authors.lastname: Liu
authors.forename: Yongxiu
authors.initials: Y
authors.lastname: Qi
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D002021:Buffers; D004563:Electrochemistry; D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D057230:Limit of Detection; D017711:Nonlinear Dynamics; D010710:Phosphates / Q000737:chemistry; D015203:Reproducibility of Results; D012997:Solvents; D013411:Sulfadiazine / Q000032:analysis*; D013420:Sulfamethoxazole / Q000032:analysis*; D013607:Tablets
chemical_list: D000890:Anti-Infective Agents; D002021:Buffers; D007202:Indicators and Reagents; D010710:Phosphates; D012997:Solvents; D013607:Tablets; D013411:Sulfadiazine; D013420:Sulfamethoxazole; C018279:sodium phosphate