title: Comparison of immune responses to the O-specific polysaccharide and lipopolysaccharide of Vibrio cholerae O1 in Bangladeshi adult patients with cholera.
abstract: Immunity against Vibrio cholerae O1 is serogroup specific, and serogrouping is defined by the O-specific polysaccharide (OSP) part of lipopolysaccharide (LPS). Despite this, human immune responses to V. cholerae OSP have not previously been characterized. We assessed immune responses against V. cholerae OSP in adults with cholera caused by V. cholerae O1 El Tor serotype Inaba or Ogawa in Dhaka, Bangladesh, using O1 OSP-core-bovine serum albumin (OSPc:BSA) conjugates; responses targeted OSP in these conjugates. Responses of Inaba-infected patients to Inaba OSP and LPS increased significantly in IgG, IgM, and IgA isotypes from the acute to convalescent phases of illness, and the responses correlated well between OSP and LPS (R = 0.86, 0.73, and 0.91, respectively; P < 0.01). Plasma IgG, IgM, and IgA responses to Ogawa OSP and LPS in Ogawa-infected patients also correlated well with each other (R = 0.60, 0.60, and 0.92, respectively; P < 0.01). Plasma IgM responses to Inaba OSP and Ogawa OSP correlated with the respective serogroup-specific vibriocidal antibodies (R = 0.80 and 0.66, respectively; P < 0.001). Addition of either OSPc:BSA or LPS, but not BSA, to vibriocidal assays inhibited vibriocidal responses in a comparable and concentration-dependent manner. Mucosal IgA immune responses to OSP and LPS were also similar. Our study is the first to characterize anti-OSP immune responses in patients with cholera and suggests that responses targeting V. cholerae LPS, including vibriocidal responses that correlate with protection against cholera, predominantly target OSP. Induction of anti-OSP responses may be associated with protection against cholera, and our results may support the development of a vaccine targeting V. cholerae OSP.
authors.affiliation: Centre for Vaccine Sciences, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh.
authors.forename: Russell A
authors.initials: RA
authors.lastname: Johnson
authors.forename: Taher
authors.initials: T
authors.lastname: Uddin
authors.forename: Amena
authors.initials: A
authors.lastname: Aktar
authors.forename: M
authors.initials: M
authors.lastname: Mohasin
authors.forename: Mohammad Murshid
authors.initials: MM
authors.lastname: Alam
authors.forename: Fahima
authors.initials: F
authors.lastname: Chowdhury
authors.forename: Jason B
authors.initials: JB
authors.lastname: Harris
authors.forename: Regina C
authors.initials: RC
authors.lastname: LaRocque
authors.forename: Meagan Kelly
authors.initials: MK
authors.lastname: Bufano
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu-Freeman
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Leung
authors.forename: David
authors.initials: D
authors.lastname: Sarracino
authors.forename: Bryan
authors.initials: B
authors.lastname: Krastins
authors.forename: Richelle C
authors.initials: RC
authors.lastname: Charles
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.forename: Pavol
authors.initials: P
authors.lastname: Kovác
authors.forename: Stephen B
authors.initials: SB
authors.lastname: Calderwood
authors.forename: Firdausi
authors.initials: F
authors.lastname: Qadri
authors.forename: Edward T
authors.initials: ET
authors.lastname: Ryan
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000097:blood*; D001459:Bangladesh; D001770:Blood Bactericidal Activity; D002771:Cholera / Q000276:immunology* / Q000382:microbiology; D005260:Female; D006801:Humans; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007075:Immunoglobulin M / Q000032:analysis / Q000097:blood; D008297:Male; D019081:O Antigens / Q000276:immunology*; D041241:Vibrio cholerae O1 / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D019081:O Antigens