title: Proteome variation among Filifactor alocis strains.
abstract: Filifactor alocis, a Gram-positive anaerobic rod, is now considered one of the marker organisms associated with periodontal disease. Although there was heterogeneity in its virulence potential, this bacterium was shown to have virulence properties that may enhance its ability to survive and persist in the periodontal pocket. To gain further insight into a possible mechanism(s) of pathogenesis, the proteome of F. alocis strains was evaluated. Proteins including several proteases, neutrophil-activating protein A and calcium-binding acid repeat protein, were identified in F. alocis. During the invasion of HeLa cells, there was increased expression of several of the genes encoding these proteins in the potentially more virulent F. alocis D-62D compared to F. alocis ATCC 35896, the type strain. A comparative protein in silico analysis of the proteome revealed more cell wall anchoring proteins in the F. alocis D-62D compared to F. alocis ATCC 35896. Their expression was enhanced by coinfection with Porphyromonas gingivalis. Taken together, the variation in the pathogenic potential of the F. alocis strains may be related to the differential expression of several putative virulence factors.
authors.affiliation: Division of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda, CA 92350, USA.
authors.forename: A Wilson
authors.initials: AW
authors.lastname: Aruni
authors.forename: Francis
authors.initials: F
authors.lastname: Roy
authors.forename: Lawrence
authors.initials: L
authors.lastname: Sandberg
authors.forename: Hansel M
authors.initials: HM
authors.lastname: Fletcher
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D016937:Gram-Positive Endospore-Forming Rods / Q000737:chemistry* / Q000378:metabolism*; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors