title: Regulatory characteristics of the Vibrio vulnificus rtxHCA operon encoding a MARTX toxin.
abstract: Vibrio vulnificus MARTX encoded by rtxA, an open reading frame of the rtxHCA operon, is essential for virulence in vitro and in mice. In this study, a primer extension analysis revealed that transcription of the rtxHCA operon begins at a single site, and is under the direction of a single promoter, P( rtxHCA ). P( rtxHCA ) activity appeared at the beginning of growth and reached a maximum in mid-exponential phase. P( rtxHCA ) activity was induced by exposure to INT-407 cells, and the membrane fraction of INT-407 cells was the most effective for the induction.
authors.affiliation: National Research Laboratory of Molecular Microbiology and Toxicology, Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Jinseo
authors.initials: J
authors.lastname: Park
authors.forename: Seung Min
authors.initials: SM
authors.lastname: Kim
authors.forename: Hee Gon
authors.initials: HG
authors.lastname: Jeong
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Choi
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001427:Bacterial Toxins / Q000378:metabolism*; D001483:Base Sequence; D002460:Cell Line; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D011401:Promoter Regions, Genetic; D014735:Vibrio Infections / Q000382:microbiology*; D041261:Vibrio vulnificus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins