title: The TSC1-TSC2 complex consists of multiple TSC1 and TSC2 subunits.
abstract: BACKGROUND
  Mutations to the TSC1 and TSC2 genes cause the disease tuberous sclerosis complex. The TSC1 and TSC2 gene products form a protein complex that integrates multiple metabolic signals to regulate the activity of the target of rapamycin (TOR) complex 1 (TORC1) and thereby control cell growth. Here we investigate the quaternary structure of the TSC1-TSC2 complex by gel filtration and coimmunoprecipitation.
  
  
  RESULTS
  TSC1 and TSC2 co-eluted in high molecular weight fractions by gel filtration. Coimmunoprecipitation of distinct tagged TSC1 and TSC2 isoforms demonstrated that TSC1-TSC2 complexes contain multiple TSC1 and TSC2 subunits.
  
  
  CONCLUSIONS
  TSC1 and TSC2 interact to form large complexes containing multiple TSC1 and TSC2 subunits.
authors.affiliation: Department of Clinical Genetics, Erasmus Medical Centre, Dr, Molewaterplein 50, Rotterdam, 3015 GE, The Netherlands.
authors.forename: Marianne
authors.initials: M
authors.lastname: Hoogeveen-Westerveld
authors.forename: Leontine
authors.initials: L
authors.lastname: van Unen
authors.forename: Ans
authors.initials: A
authors.lastname: van den Ouweland
authors.forename: Dicky
authors.initials: D
authors.lastname: Halley
authors.forename: Andre
authors.initials: A
authors.lastname: Hoogeveen
authors.forename: Mark
authors.initials: M
authors.lastname: Nellist
mesh_terms: D002850:Chromatography, Gel; D000939:Epitopes; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D020836:Protein Structure, Quaternary; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D000077004:Tuberous Sclerosis Complex 1 Protein; D000077005:Tuberous Sclerosis Complex 2 Protein; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000939:Epitopes; D020033:Protein Isoforms; C000624650:TSC1 protein, human; C000624653:TSC2 protein, human; D000077004:Tuberous Sclerosis Complex 1 Protein; D000077005:Tuberous Sclerosis Complex 2 Protein; D025521:Tumor Suppressor Proteins; D058570:TOR Serine-Threonine Kinases