title: Effect of ozone application on the immediate shear bond strength and microleakage of dental sealants.
abstract: OBJECTIVE
  The purpose of this study was to evaluate whether gaseous ozone application affects the immediate enamel bond strength or microleakage of 2 dental sealants.
  
  
  METHODS
  Sixty bovine incisors were randomly divided into 4 groups, and sealants were applied to the enamel surfaces as follows: ozone + Concise; Concise (control); ozone + UltraSeal XT Plus; and UltraSeal XT Plus (control). Ozone application was performed for 80 seconds, and shear bond strength was measured. Additionally, 60 human molars were randomly divided into 4 groups, as aforementioned, and sealants were applied onto occlusal surfaces. Dye penetration (microleakage) was assessed.
  
  
  RESULTS
  No significant differences were found between the 2 sealants. Ozone application did not result in a significant reduction in enamel bond strength or an increase in microleakage.
  
  
  CONCLUSIONS
  Ozone gas did not compromise the adhesion of tested materials; therefore, one can disinfect the enamel surface before placing a dental sealant without impairing the material's clinical performance.
authors.affiliation: Department of Medical Sciences, University of Trieste, Trieste, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Marchesi
authors.forename: Lorenza Clara
authors.initials: LC
authors.lastname: Petris
authors.forename: Chiara Ottavia
authors.initials: CO
authors.lastname: Navarra
authors.forename: Ranieri
authors.initials: R
authors.lastname: Locatelli
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Lenarda
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Breschi
authors.forename: Milena
authors.initials: M
authors.lastname: Cadenaro
mesh_terms: D000818:Animals; D002417:Cattle; D010126:Ozone / Q000008:administration & dosage*; D010895:Pit and Fissure Sealants
chemical_list: D010895:Pit and Fissure Sealants; D010126:Ozone