title: Plant growth promoting bacteria in Brachiaria brizantha.
abstract: Brachiaria brizantha is considered one of the preferred fodders among farmers for having high forage yield and large production of root mass. The association of beneficial bacteria with these grasses can be very valuable in the recovery of the pasture areas with nutritional deficiency. With the aim of studying this possibility, we carried out the sampling of soil and roots of B. brizantha in three areas (Nova Odessa-SP, São Carlos-SP and Campo Verde-MT, Brazil). Seventy-two bacterial strains were isolated and used in tests to evaluate their biotechnological potential. Almost all isolates presented at least one positive feature. Sixty-eight isolates produced analogues of indole-3-acetic acid, ten showed nitrogenase activity when subjected to the method of increasing the concentration of total nitrogen (total N) in the culture medium and sixty-five isolates showed nitrogenase activity when subjected to acetylene reduction technique. The partial sequencing of 16S rRNA of these isolates allowed the identification of seven main groups, with the prevalence of those affiliated to the genus Stenotrophomonas (69 %). At the end, this work elected the strains C4 (Pseudomonadaceae) and C7 (Rhodospirillaceae) as promising organisms for the development of inoculants due to their higher nitrogenase activity.
authors.affiliation: Department of Soil Science, Laboratory of Soil Microbiology, São Paulo State University/College of Agriculture, Av. Pádua Dias, 11, CP 09, Piracicaba, SP 13418-900, Brazil. mylennepi@gmail.com
authors.forename: Mylenne Calciolari Pinheiro
authors.initials: MC
authors.lastname: Silva
authors.forename: Aline Fernandes
authors.initials: AF
authors.lastname: Figueiredo
authors.forename: Fernando Dini
authors.initials: FD
authors.lastname: Andreote
authors.forename: Elke Jurandy Bran Nogueira
authors.initials: EJ
authors.lastname: Cardoso
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D035201:Brachiaria / Q000254:growth & development* / Q000382:microbiology*; D007210:Indoleacetic Acids / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism; D009591:Nitrogenase / Q000378:metabolism; D063245:Plant Development / Q000235:genetics; D010937:Plant Growth Regulators / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D006109:Poaceae / Q000254:growth & development / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil; D012988:Soil Microbiology
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S; D012987:Soil; C030737:indoleacetic acid; D009591:Nitrogenase; D009584:Nitrogen