title: Rapid low-cost detection of Klebsiella pneumoniae carbapenemase genes by internally controlled real-time PCR.
abstract: An internally controlled real-time PCR assay based on SYBR Green was developed to screen Klebsiella pneumoniae carbapenemases (KPCs) gene containing bacteria and was validated for clinical strains or surveillance specimens. When 248 clinical samples were tested, the sensitivity and specificity of the assay were 100% and 99%, respectively.
authors.affiliation: Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
authors.forename: Haitong
authors.initials: H
authors.lastname: Gu
authors.forename: Xinxin
authors.initials: X
authors.lastname: Lu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase