title: The response of cementoblasts to calcium phosphate resin-based and calcium silicate-based commercial sealers.
abstract: OBJECTIVE
  To investigate cell viability and gene expression of cementoblasts (OCCM.30) exposed to extractable components released by resin-based sealers with different chemical composition Hybrid Root Seal (HRS), SimpliSeal (SS), Real Seal (RS) and AH Plus (AH) and by a MTA-based sealers Tech Biosealer Endo (TBE).
  
  
  METHODS
  Discs of all materials were prepared and allowed to set in humid conditions at 37° for 48 h. The discs were then incubated for 72 h at 37 °C to obtain material extracts (1/1) in DMEM. The extracts containing the components released by the sealers were filtered and other dilutions (1/2, 1/4) were prepared from the original solution (1/1). Original and diluted solutions were tested on the cementoblasts. Impedance-based real-time cell analysis (RTCA) was used to evaluate cell viability, quantitative real-time polymerase chain reaction (QRT-PCR) was used to examine the expression of mineralization-related genes (osteocalcin; OCN, Runt-related transcription factor-2; Runx2, collagen type 1; COL I, alkaline phosphatase; ALP). For statistical analysis, one-way analysis of variance (anova) and Tukey's honestly significant difference (HSD) tests were used.
  
  
  RESULTS
  TBE (1/2), RS (1/2, 1/4), and HRS (1/2, 1/4) significantly decreased cell viability (P < 0.001). AH (1/2, 1/4) and SS (1/2, 1/4) had similar cell viability to the control at 30 h. All tested materials significantly decreased cell viability when compared to the control group except AH (1/2, 1/4) and SS (1/4) at 90 h. All of the tested sealers reduced COL I mRNA expressions when compared to the control. SS was associated with significant increases in OCN and Runx2 mRNA expressions when compared to the control (P < 0.001). Whereas all of the dilutions of TBE, RS and HRS significantly decreased BSP mRNA expressions (P < 0,001), 1/2 and 1/4 dilutions of SS increased BSP mRNA expression (P < 0,001). Except the 1/4 dilutions of AH and SS, all the sealer dilutions significantly reduced ALP mRNA expression in cementoblasts (P < 0,001).
  
  
  CONCLUSIONS
  SimpliSeal and AH Plus resulted in more favourable response to cementoblasts because of their regulation potential on the mineralized tissue-associated protein's mRNA expressions.
authors.affiliation: Department of Periodontology, Selcuk University, Konya, Turkey.
authors.forename: S S
authors.initials: SS
authors.lastname: Hakki
authors.forename: B S
authors.initials: BS
authors.lastname: Bozkurt
authors.forename: B
authors.initials: B
authors.lastname: Ozcopur
authors.forename: M G
authors.initials: MG
authors.lastname: Gandolfi
authors.forename: C
authors.initials: C
authors.lastname: Prati
authors.forename: S
authors.initials: S
authors.lastname: Belli
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D017607:Aluminum Compounds / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology*; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000032:analysis; D003188:Composite Resins / Q000737:chemistry / Q000494:pharmacology; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D003739:Dental Cementum / Q000187:drug effects*; D004338:Drug Combinations; D004853:Epoxy Resins / Q000737:chemistry / Q000494:pharmacology; D006813:Humidity; D058574:Integrin-Binding Sialoprotein / Q000032:analysis; D051379:Mice; D015675:Osteocalcin / Q000032:analysis; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D019279:Resin Cements / Q000737:chemistry / Q000494:pharmacology*; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology*; D012823:Silicate Cement / Q000737:chemistry / Q000494:pharmacology*; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors
chemical_list: D017607:Aluminum Compounds; C097428:Bioseal; D017610:Calcium Compounds; D002130:Calcium Phosphates; D024042:Collagen Type I; D003188:Composite Resins; D050659:Core Binding Factor Alpha 1 Subunit; D004338:Drug Combinations; D004853:Epoxy Resins; C546872:Ibsp protein, mouse; D058574:Integrin-Binding Sialoprotein; D010087:Oxides; D012333:RNA, Messenger; C511498:Real Seal composite resin; D019279:Resin Cements; D012387:Root Canal Filling Materials; C493716:Runx2 protein, mouse; D017640:Silicates; C534916:epoxy resin-based root canal sealer; C086631:mineral trioxide aggregate; D015675:Osteocalcin; D012823:Silicate Cement; C020243:calcium phosphate; D000469:Alkaline Phosphatase; C031293:calcium silicate