title: Microarrays for universal detection and identification of phytoplasmas.
abstract: Detection and identification of phytoplasmas is a laborious process often involving nested PCR followed by restriction enzyme analysis and fine-resolution gel electrophoresis. To improve throughput, other methods are needed. Microarray technology offers a generic assay that can potentially detect and differentiate all types of phytoplasmas in one assay. The present protocol describes a microarray-based method for identification of phytoplasmas to 16Sr group level.
authors.affiliation: Department of Agroecology, Aarhus University, Slagelse, Denmark. mogens.nicolaisen@agrsci.dk
authors.forename: Mogens
authors.initials: M
authors.lastname: Nicolaisen
authors.forename: Henriette
authors.initials: H
authors.lastname: Nyskjold
authors.forename: Assunta
authors.initials: A
authors.lastname: Bertaccini
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D045582:Phytoplasma / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S