title: Cooperation of two bifunctional enzymes in the biosynthesis and attachment of deoxysugars of the antitumor antibiotic mithramycin.
abstract: Two bifunctional enzymes cooperate in the assembly and the positioning of two sugars, D-olivose and D-mycarose, of the anticancer antibiotic mithramycin. MtmC finishes the biosynthesis of both sugar building blocks depending on which MtmGIV activity is supported. MtmGIV transfers these two sugars onto two structurally distinct acceptor substrates. The dual function of these enzymes explains two essential but previously unidentified activities.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536, USA.
authors.forename: Guojun
authors.initials: G
authors.lastname: Wang
authors.forename: Pallab
authors.initials: P
authors.lastname: Pahari
authors.forename: Madan K
authors.initials: MK
authors.lastname: Kharel
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Haining
authors.initials: H
authors.lastname: Zhu
authors.forename: Steven G
authors.initials: SG
authors.lastname: Van Lanen
authors.forename: Jürgen
authors.initials: J
authors.lastname: Rohr
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D002240:Carbohydrate Sequence; D003837:Deoxy Sugars / Q000096:biosynthesis / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000096:biosynthesis / Q000378:metabolism*; D008969:Molecular Sequence Data; D008926:Plicamycin / Q000096:biosynthesis*
chemical_list: D000903:Antibiotics, Antineoplastic; D003837:Deoxy Sugars; D016695:Glycosyltransferases; D008926:Plicamycin