title: [Effects of omeprazole on the proliferation and apoptosis of hepatoma cell line HepG2].
abstract: OBJECTIVE
  To investigate the effects of omeprazole (OME), a proton pump inhibitor, on the proliferation and apoptosis of human hepatoma cell line HepG2.
  
  
  METHODS
  HepG2 cells were cultured to the logarithmic phase, and then treated with OME of different concentrations (10, 20, 40, 80, 160 mg/L) for 24 h or 48 h. Cell proliferation was evaluated by MTT assay, DNA synthesis was measured with 5-ethynyl-2'-deoxyuridine (Edu) fluorescent assay and the apoptosis of cells was measured by the Hoechst33342 assay.
  
  
  RESULTS
  MTT assay showed that OME (40, 80 and 160 mg/L concentrations) could inhibit the proliferation of HepG2 cells for 24 h or 48 h treatment (P < 0.05) and 80 mg/L group has strongest effect. Compared with that of 24 h treatment, the same concentration of OME could inhibit HepG2 more significantly with 48 h treatment. After different concentrations of OME treatment for 24 h and then incubation with Edu for 2 h, compared with the control group, the proportion of Cells in S phase in 20, 40, 80, 160 mg/L groups decreased. Hoechst33342 staining demonstrated that treatment with OME (40, 80,160 mg/L) for 24 h could significantly promote the cell apoptosis.
  
  
  CONCLUSIONS
  Omeprazole could inhibit human hepatoma cell line HepG2 cell proliferation and promote apoptosis.
authors.affiliation: Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.forename: Ning-Lin
authors.initials: NL
authors.lastname: Liang
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Zhu
authors.forename: Wen-Chao
authors.initials: WC
authors.lastname: Wu
authors.forename: Xiao-Jing
authors.initials: XJ
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Yang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D009853:Omeprazole / Q000494:pharmacology*; D054328:Proton Pump Inhibitors / Q000494:pharmacology
chemical_list: D054328:Proton Pump Inhibitors; D009853:Omeprazole