title: [Effects of acrolein on apoptosis of H9c2 cardiacmyocytes with hypoxia/reoxygenation injury].
abstract: OBJECTIVE
  To determine the cytotoxic effects of acrolein on hypoxia/reoxygenation (H/R) injury in H9c2 cardiacmyocytes and investigate the intracellular signaling pathways.
  
  
  METHODS
  Hypoxia/reoxygenation (H/R) injury model was established with H9c2 cells. The H9c2 cells were divided into four groups, the control group, acrolein group (ACR), H/R group, acrolein + H/R group (ACR + H/R). H9c2 cells pretreated with or without acrolein (10 micromol/L) for 30 min were exposed to 2 h hypoxia and 16 h reoxygenation. The effect of acrolein on the cellular viability and apoptosis of H9c2 cells was measured by MTT assay, DAPI stainning and flow cytometry (FCM) respectively. The expression of apotosis-related proteins (cytochrome c, caspase 9 and caspase 3) in the H9c2 cells was detected by Western blot.
  
  
  RESULTS
  Compared with mere H/R treatment, the decrease in cell viability and increase in the number of apoptotic cells in H9c2 cells subjected to H/R were significantly exacerbated in the presence of acrolein (P < 0.05). The liberation of cytochrome c from mitochondria to cytosol, the cleavages of the initiator caspase 9 and the effector caspase 3 have been observed after pretreatment with acrolein followed by H/ R in H9c2 cells.
  
  
  CONCLUSIONS
  Acrolein could aggravate H/R injury and that this effect may be related, in part, to the modification of proteins involved the release of cytochrome c from mitochondria to cytosol and activation of caspases cascade reaction.
authors.affiliation: Institute for Nanobiomedical Technology and Memberane Biology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Qing-Rui
authors.initials: QR
authors.lastname: Shao
authors.forename: Dan
authors.initials: D
authors.lastname: Du
authors.forename: Yang
authors.initials: Y
authors.lastname: He
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Wu
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Xing
mesh_terms: D000171:Acrolein / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D015687:Cell Hypoxia / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects / Q000473:pathology*; D015427:Reperfusion Injury / Q000503:physiopathology*
chemical_list: D000171:Acrolein