title: The role of TRB3 in mast cells sensitized with monomeric IgE.
abstract: Mast cells play a key role in immunoglobulin E (IgE)-associated allergic disorders; however, the cellular effects of sensitization remain poorly understood. Using gene microarrays and the multiplexing ELISA method, we examined the effects of sensitization on RBL-CCR1 cell transcription and chemokine/cytokine secretion. Sensitization most prominently increased transcription of Trb3, the gene for tribbles homolog 3 (TRB3), and also increased the release of most of the cytokines and chemokines tested. Knockdown of TRB3 via RNAi significantly induced the production of tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), interleukin-6 (IL-6), and the chemokine mast cell protease-1 (MCP-1). TRB3 deficiency also induced IκBα phosphorylation. TRB3 may therefore serve as a negative regulator of pro-inflammatory cytokines and chemokines, controlling the extent of the inflammatory response.
authors.affiliation: Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, OH 45221-0097, USA.
authors.forename: Chuan-Hui
authors.initials: CH
authors.lastname: Kuo
authors.forename: Kei
authors.initials: K
authors.lastname: Morohoshi
authors.forename: Cho Cho
authors.initials: CC
authors.lastname: Aye
authors.forename: Robert B
authors.initials: RB
authors.lastname: Garoon
authors.forename: Andrea
authors.initials: A
authors.lastname: Collins
authors.forename: Santa Jeremy
authors.initials: SJ
authors.lastname: Ono
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D018925:Chemokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000502:physiology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D018925:Chemokines; D034741:RNA, Small Interfering; C502443:Trib3 protein, rat; D007073:Immunoglobulin E; D017346:Protein Serine-Threonine Kinases