title: Exposure to ionizing radiation induces the migration of cutaneous dendritic cells by a CCR7-dependent mechanism.
abstract: In the event of a deliberate or accidental radiological emergency, the skin would likely receive substantial ionizing radiation (IR) poisoning, which could negatively impact cellular proliferation, communication, and immune regulation within the cutaneous microenvironment. Indeed, as we have previously shown, local IR exposure to the murine ear causes a reduction of two types of cutaneous dendritic cells (cDC), including interstitial dendritic cells of the dermis and Langerhans cells of the epidermis, in a dose- and time-dependent manner. These APCs are critical regulators of skin homeostasis, immunosurveillance, and the induction of T and B cell-mediated immunity, as previously demonstrated using conditional cDC knockout mice. To mimic a radiological emergency, we developed a murine model of sublethal total body irradiation (TBI). Our data would suggest that TBI results in the reduction of cDC from the murine ear that was not due to a systemic response to IR, as a loss was not observed in shielded ears. We further determined that this reduction was due, in part, to the upregulation of the chemoattractant CCL21 on lymphatic vessels as well as CCR7 expressed on cDC. Migration as a potential mechanism was confirmed using CCR7(-/-) mice in which cDC were not depleted following TBI. Finally, we demonstrated that the loss of cDC following TBI results in an impaired contact hypersensitivity response to hapten by using a modified contact hypersensitivity protocol. Taken together, these data suggest that IR exposure may result in diminished immunosurveillance in the skin, which could render the host more susceptible to pathogens.
authors.affiliation: Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Cummings
authors.forename: Scott A
authors.initials: SA
authors.lastname: Gerber
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Judge
authors.forename: Julie L
authors.initials: JL
authors.lastname: Ryan
authors.forename: Alice P
authors.initials: AP
authors.lastname: Pentland
authors.forename: Edith M
authors.initials: EM
authors.lastname: Lord
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000276:immunology* / Q000528:radiation effects*; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology / Q000528:radiation effects*; D003877:Dermatitis, Contact / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D004423:Ear; D005260:Female; D005720:Gamma Rays; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D054400:Receptors, CCR7 / Q000502:physiology* / Q000528:radiation effects; D012867:Skin / Q000276:immunology* / Q000473:pathology / Q000528:radiation effects*; D014916:Whole-Body Irradiation / Q000009:adverse effects
chemical_list: C515371:Ccr7 protein, mouse; D054400:Receptors, CCR7