title: Conjugated linoleic acids alleviate the immunosuppression of peripheral blood T lymphocytes in broiler chickens exposed to cyclosporin A.
abstract: The immunoregulatory actions of conjugated linoleic acids (CLA) of relevance immunosuppression were investigated. To test the hypothesis that CLA ameliorate immunosuppression, we developed the immunosuppressive model of peripheral blood T lymphocytes in broiler chickens induced by cyclosporin A. Peripheral blood T lymphocytes of broiler chickens were cultured with media containing various concentrations (25, 50, 100, and 200 μmol/L) of c9, t11-CLA and t10, c12-CLA to investigate the effects of CLA isomers on peripheral blood T lymphocyte proliferation, interleukin-2, the activity of phospholipase C, and protein kinase C production. Results suggested that CLA alleviated the immunosuppression of T lymphocytes in broiler chickens exposed to cyclosporin A through increasing of peripheral blood T lymphocyte proliferation and interleukin-2. The 2 CLA isomers enhanced T lymphocyte proliferation at low concentration and inhibited T lymphocyte proliferation at high concentration. In addition, the effect of c9, t11-CLA was better than that of t10, c12-CLA. At the cellular level, the effects of CLA on the alleviation of immunosuppression in T lymphocytes are mainly attributable to increasing the signaling molecules, such as phospholipase C and protein kinase C.
authors.affiliation: College of Animal Science and Technology, China Agricultural University, Beijing, People's Republic of China.
authors.forename: F Y
authors.initials: FY
authors.lastname: Long
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: Y M
authors.initials: YM
authors.lastname: Guo
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: J M
authors.initials: JM
authors.lastname: Yuan
authors.forename: B K
authors.initials: BK
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Liu
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D049109:Cell Proliferation; D002645:Chickens; D016572:Cyclosporine / Q000494:pharmacology*; D004032:Diet / Q000662:veterinary*; D007165:Immunosuppression Therapy / Q000662:veterinary*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D008297:Male; D054801:Phosphoinositide Phospholipase C / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
chemical_list: D007166:Immunosuppressive Agents; D007376:Interleukin-2; D044243:Linoleic Acids, Conjugated; D016572:Cyclosporine; D054801:Phosphoinositide Phospholipase C