title: [HPLC-ELSD fingerprint of Marsdenia tenacissima from different habitats].
abstract: OBJECTIVE
  To establish a HPLC-ELSD fingerprint of Marsdenia tenacissima from different habitats, in order to provide a reliable method for scientific assessment and effective quality control.
  
  
  METHODS
  HPLC-ELSD was adopted to determine 25 baches of M. tenacissima herbs from different habitats. Traditional Chinese medicine chromatographic fingerprint similarity software assessment system 2004 developed by China Pharmacopoeia Committee was adopted to establish a common mode chart and assess chromatographic similarity based on the degree of correlation.
  
  
  RESULTS
  The common mode for M. tenacissima herb C21 steroidal fingerprint was established, including 11 common characteristic peaks. Among them, 10 were identified. According to the assessment on the similarity of 25 batches of samples, 80% of them showed a similarity of over 0.80 in steroidal HPLC-ELSD fingerprint.
  
  
  CONCLUSIONS
  The method can be used to assess the quality of M. tenacissima.
authors.affiliation: Nanjing Sanhome pharmaceutical Co., Ltd., Nanjing 210038, China. lien@sanhome.com
authors.forename: Erna
authors.initials: E
authors.lastname: Li
authors.forename: Zaijin
authors.initials: Z
authors.lastname: Teng
authors.forename: Shiping
authors.initials: S
authors.lastname: Liu
authors.forename: Song
authors.initials: S
authors.lastname: Qin
authors.forename: Cang
authors.initials: C
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D017753:Ecosystem; D008027:Light; D031101:Marsdenia / Q000737:chemistry* / Q000145:classification*; D012542:Scattering, Radiation
chemical_list: D004365:Drugs, Chinese Herbal