title: Filtration, adsorption and immunodetection of virus using polyelectrolyte multilayer-modified paper.
abstract: A new method for detection of viruses has been developed. The entire assay can be performed within 2h, and consists of a polyelectrolyte-multilayer-modified cellulosic filter paper combined with immunodetection. The M13 bacteriophage was used as a model virus. A visual colour-based detection system, anti-M13 horseradish peroxidase (HRP) conjugate and 3,3',5,5'-tetramethylbenzidine (TMB), was selected to allow semi-quantitative assessment by human eye, or quantitative assessment using a digital scanner. By filtering a volume of 0.50 ml, it was possible to visually detect a concentration of 10(6) pfu/ml. The detection limit was improved to 5×10(4) pfu/ml by increasing the volume of the sample to 100ml. For comparison, it was only possible to detect a concentration of 10(7) pfu/ml using conventional sandwich enzyme-linked immunosorbent assay (ELISA) with the same detection system.
authors.affiliation: Department of Physics, McGill University, Montréal, QC, Canada. per.larsson@polymer.kth.se
authors.forename: Per A
authors.initials: PA
authors.lastname: Larsson
authors.forename: Sushma
authors.initials: S
authors.lastname: G Puttaswamaiah
authors.forename: Christine
authors.initials: C
authors.lastname: Ly
authors.forename: Alois
authors.initials: A
authors.lastname: Vanerek
authors.forename: J Christopher
authors.initials: JC
authors.lastname: Hall
authors.forename: François
authors.initials: F
authors.lastname: Drolet
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000327:Adsorption; D017104:Bacteriophage M13 / Q000737:chemistry / Q000302:isolation & purification; D001560:Benzidines / Q000737:chemistry; D002482:Cellulose; D004563:Electrochemistry; D004573:Electrolytes / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D006735:Horseradish Peroxidase / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D010209:Paper; D011073:Polyamines / Q000737:chemistry; D011108:Polymers; D014780:Viruses / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D001560:Benzidines; D004573:Electrolytes; D011073:Polyamines; D011108:Polymers; C063994:polyallylamine; C021758:3,3',5,5'-tetramethylbenzidine; C006903:carbopol 940; D002482:Cellulose; D006735:Horseradish Peroxidase