title: Cell-based delivery of glucagon-like peptide-1 using encapsulated mesenchymal stem cells.
abstract: Glucagon-like peptide-1 (GLP-1) CellBeads are cell-based implants for the sustained local delivery of bioactive factors. They consist of GLP-1 secreting mesenchymal stem cells encapsulated in a spherically shaped immuno-isolating alginate matrix. A highly standardized and reproducible encapsulation method is described for the manufacturing of homogeneous CellBeads. Viability and sustained secretion was shown for the recombinant GLP-1 and the cell endogenous bioactive factors like vascular endothelial growth factor, neurotrophin 3 (NT-3) and glial cell line-derived neurotrophic factor. Manufacturing and quality control is performed in compliance with good manufacturing practice and fulfils all regulatory requirements for human clinical use. GLP-1 CellBeads combine the neuro- and cardioprotective properties of both GLP-1 and mesenchymal stem cells. First promising results were obtained from preclinical studies and an ongoing safety trial in humans but further studies have to prove the overall potential of CellBead technology in cell-based regenerative medicine.
authors.affiliation: CellMed AG, a BTG Company, 63755 Alzenau, Germany. christine.wallrapp@cellmed.de
authors.forename: Christine
authors.initials: C
authors.lastname: Wallrapp
authors.forename: Eric
authors.initials: E
authors.lastname: Thoenes
authors.forename: Frank
authors.initials: F
authors.lastname: Thürmer
authors.forename: Anette
authors.initials: A
authors.lastname: Jork
authors.forename: Moustapha
authors.initials: M
authors.lastname: Kassem
authors.forename: Peter
authors.initials: P
authors.lastname: Geigle
mesh_terms: D000464:Alginates / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D045744:Cell Line, Tumor; D018914:Cells, Immobilized; D004337:Drug Carriers; D016228:Endothelial Growth Factors / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1 / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D020933:Neurotrophin 3 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D011994:Recombinant Proteins
chemical_list: D000464:Alginates; D004337:Drug Carriers; D016228:Endothelial Growth Factors; D006603:Hexuronic Acids; D020933:Neurotrophin 3; D011994:Recombinant Proteins; D052216:Glucagon-Like Peptide 1; D020723:Glucuronic Acid