title: Characterization of corrinoid compounds from edible cyanobacterium Nostochopsis sp.
abstract: Vitamin B₁₂ content of an edible cyanobacterium, Nostochopsis sp. was determined to be 140.6±16.2 μg/100 g dry weight by a microbiological method. To evaluate whether the Nostochopsis cells contain vitamin B₁₂ or inactive corrinoid compounds, corrinoid compounds were purified from the cells and then identified as pseudovitamin B₁₂ (97.4±11.8 μg/100 g dry weight) and vitamin B₁₂ (43.2±6.0 μg/100 g dry weight) on the basis of silica gel 60 TLC bioautograms and LC/ESI-MS/MS chromatograms. Vitamin B₁₂ content was significantly increased in the Nostochopsis cells (254.8±17.6 μg/100 g dry weight) grown in the vitamin B₁₂-supplemented medium.
authors.affiliation: School of Agricultural, Biological and Environmental Sciences, Tottori University, Tottori, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Hashimoto
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Yabuta
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takenaka
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takenaka
authors.forename: Fumio
authors.initials: F
authors.lastname: Watanabe
mesh_terms: D000458:Cyanobacteria / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: C005602:pseudovitamin B12; D014805:Vitamin B 12