title: Unravelling mitochondrial retrograde regulation in the abiotic stress induction of rice ALTERNATIVE OXIDASE 1 genes.
abstract: Mitochondrial retrograde regulation (MRR) is the transduction of mitochondrial signals to mediate nuclear gene expression. It is not clear whether MRR is a common regulation mechanism in plant abiotic stress response. In this study, we analysed the early abiotic stress response of the rice OsAOX1 genes, and the induction of OsAOX1a and OsAOX1b (OsAOX1a/b) was selected as a working model for the stress-induced MRR studies. We found that the induction mediated by the superoxide ion (O2·(-) )-generating chemical methyl viologen was stronger than that of hydrogen peroxide (H2 O2 ). The addition of reactive oxygen species (ROS) scavengers demonstrated that the stress induction was reduced by eliminating O2·(-) . Furthermore, the stress induction did not rely on chloroplast- or cytosol-derived O2·(-) . Next, we generated transgenic plants overexpressing the superoxide dismutase (SOD) gene at different subcellular locations. The results suggest that only the mitochondrial SOD, OsMSD, attenuated the stress induction of OsAOX1a/b specifically. Therefore, our findings demonstrate that abiotic stress initiates the MRR on OsAOX1a/b and that mitochondrial O2·(-) is involved in the process.
authors.affiliation: Institute of Rice Research, Anhui Academy of Agricultural Science, Hefei 230031, China.
authors.forename: Chun-Rong
authors.initials: CR
authors.lastname: Li
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Liang
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Yong-Bo
authors.initials: YB
authors.lastname: Duan
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Ya-Chun
authors.initials: YC
authors.lastname: Yang
authors.forename: Rui-Ying
authors.initials: RY
authors.lastname: Qin
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Wei
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Yang
mesh_terms: D002118:Calcium / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003080:Cold Temperature; D055864:Droughts; D016166:Free Radical Scavengers / Q000494:pharmacology; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology* / Q000502:physiology; D024101:Mitochondrial Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D010269:Paraquat / Q000494:pharmacology*; D010940:Plant Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D017382:Reactive Oxygen Species / Q000494:pharmacology; D054712:Salinity; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D013481:Superoxides / Q000032:analysis / Q000494:pharmacology; D000099188:Alternative Oxidase
chemical_list: D002118:Calcium; D016166:Free Radical Scavengers; D006861:Hydrogen Peroxide; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010269:Paraquat; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D013481:Superoxides; D000099188:Alternative Oxidase