title: Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus.
abstract: This report describes the cloning, characterization and gene expression pattern of two Th17 cytokines, interleukin (IL)-17A/F and -22, in turbot Scophthalmus maximus. The turbot IL-17A/F cDNA contains a 516 bp open reading frame encoding a deduced IL-17A/F protein of 171 amino acid (aa) residues, containing a predicted signal peptide of 31 aa. Turbot IL-22 had a 564 bp ORF coding for a 187 aa protein with a 33 aa signal peptide. The turbot IL-22 protein contained a typical IL-10 family signature. Both cytokines had highest expression levels in the intestine followed by head kidney and gills. Stimulation with the Gram negative bacterium Aeromonas salmonicida was able to modulate IL-17A/F and IL-22 expression in head kidney, spleen and liver but not the intestine. PMA and PHA were also able to induce the expression of both cytokines, suggesting that, as expected, T-cells are likely the main producers of these molecules in turbot as in mammals.
authors.affiliation: Instituto de Investigaciones Marinas, CSIC, Vigo, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: Costa
authors.forename: P
authors.initials: P
authors.lastname: Pereiro
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: C J
authors.initials: CJ
authors.lastname: Secombes
authors.forename: A
authors.initials: A
authors.lastname: Figueras
authors.forename: B
authors.initials: B
authors.lastname: Novoa
mesh_terms: D048409:Aeromonas salmonicida / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D017756:Aquaculture; D001483:Base Sequence; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005412:Flatfishes / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D015870:Gene Expression; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D060226:Head Kidney / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D020381:Interleukin-17 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007378:Interleukins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008099:Liver / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D040681:Structural Homology, Protein; D058504:Th17 Cells / Q000378:metabolism; D000097043:Interleukin-22
chemical_list: D029941:Fish Proteins; D020381:Interleukin-17; D007378:Interleukins; D000097043:Interleukin-22