title: Investigating the interactions between cations, peroxidation substrates and G-quadruplex topology in DNAzyme peroxidation reactions using statistical testing.
abstract: Cations affect the topology and enzymatic proficiency of most macromolecular catalysts but the role of cations in DNAzyme peroxidation reactions remains unresolved. Herein, we use statistical methods (ANOVA, t-test and Wilcoxon Mann-Whitney non-parametric test) to demonstrate that there are strong associations between cations, DNAzyme topology, peroxidation substrate and peroxidation rates of G-quadruplex peroxidises. Ammonium cation was found to be superior to all tested cations, including potassium. A t-test indicated that NH(4)(+) was better than K(+) with a p-value=0.05. Interestingly, the nature of the peroxidation substrate employed affected the dependence of peroxidation rate on the cation present and of the three substrates tested, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), tyramine and 3,3',5,5'-tetramethylbenzidine (TMB), ABTS was the most sensitive to the nature of cation present.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
authors.forename: Shizuka
authors.initials: S
authors.lastname: Nakayama
authors.forename: Herman O
authors.initials: HO
authors.lastname: Sintim
mesh_terms: D000704:Analysis of Variance; D001560:Benzidines / Q000737:chemistry; D052160:Benzothiazoles / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D002412:Cations / Q000737:chemistry / Q000378:metabolism; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes; D006427:Hemin / Q000737:chemistry; D009195:Peroxidase / Q000737:chemistry; D011188:Potassium / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D018709:Statistics, Nonparametric; D013451:Sulfonic Acids / Q000737:chemistry / Q000378:metabolism*; D014439:Tyramine / Q000737:chemistry
chemical_list: D001560:Benzidines; D052160:Benzothiazoles; D002412:Cations; D021881:DNA, Catalytic; D000644:Quaternary Ammonium Compounds; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C021758:3,3',5,5'-tetramethylbenzidine; D006427:Hemin; D009195:Peroxidase; D011188:Potassium; D014439:Tyramine