title: Structural insight into the interaction of ADP-ribose with the PARP WWE domains.
abstract: The WWE domain is often identified in proteins associated with ubiquitination or poly-ADP-ribosylation. Structural information about WWE domains has been obtained for the ubiquitination-related proteins, such as Deltex and RNF146, but not yet for the poly-ADP-ribose polymerases (PARPs). Here we determined the solution structures of the WWE domains from PARP11 and PARP14, and compared them with that of the RNF146 WWE domain. NMR perturbation experiments revealed the specific differences in their ADP-ribose recognition modes that correlated with their individual biological activities. The present structural information sheds light on the ADP-ribose recognition modes by the PARP WWE domains.
authors.affiliation: RIKEN Systems and Structural Biology Center, Tsurumi-ku, Yokohama, Kanagawa, Japan.
authors.forename: Fahu
authors.initials: F
authors.lastname: He
authors.forename: Kengo
authors.initials: K
authors.lastname: Tsuda
authors.forename: Mari
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kanako
authors.initials: K
authors.lastname: Kuwasako
authors.forename: Takaho
authors.initials: T
authors.lastname: Terada
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Satoru
authors.initials: S
authors.lastname: Watanabe
authors.forename: Takanori
authors.initials: T
authors.lastname: Kigawa
authors.forename: Naohiro
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Peter
authors.initials: P
authors.lastname: Güntert
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Muto
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003198:Computer Simulation; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011065:Poly(ADP-ribose) Polymerases / Q000138:chemical synthesis* / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D044767:Ubiquitin-Protein Ligases / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000246:Adenosine Diphosphate Ribose; D044767:Ubiquitin-Protein Ligases; C522628:Parp14 protein, mouse; D011065:Poly(ADP-ribose) Polymerases; C000605039:poly(ADP-ribose) polymerase-11, mouse