title: Development and characterization of 59 polymorphic cDNA-SSR markers for the edible oil crop Sesamum indicum (Pedaliaceae).
abstract: OBJECTIVE
  Polymorphic simple sequence repeat markers from transcript sequences (cDNA-simple sequence repeat [SSR]) were developed for the edible oil crop Sesamum indicum to facilitate the genetic study of this species. •
  
  
  RESULTS
  We found 7702 SSR loci in the 60960 unigenes, and 1550 primer pairs were designed and synthesized. In total, 59 primer pairs showed polymorphism within 36 individuals; the number of alleles per locus ranged from two to four, and the expected and observed heterozygosity ranged from 0.05 to 0.74 and 0 to 0.30, respectively. •
  
  
  CONCLUSIONS
  These polymorphic markers will greatly facilitate studies of the genetic structure of S. indicum populations as well as the identification and conservation of the species.
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, People's Republic of China.
authors.forename: Linhai
authors.initials: L
authors.lastname: Wang
authors.forename: Yanxin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoqiong
authors.initials: X
authors.lastname: Qi
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiurong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D018556:Crops, Agricultural / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D010938:Plant Oils / Q000378:metabolism*; D010945:Plants, Edible / Q000235:genetics*; D011110:Polymorphism, Genetic; D031685:Sesamum / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D005819:Genetic Markers; D010938:Plant Oils