title: Reverse transcription-PCR for phytoplasma detection utilizing crude sap extractions.
abstract: Phytoplasmas are routinely detected by nucleic acid-based techniques. These approaches rely on enriched phytoplasma DNA extracts of good quality, following labor intensive and time-consuming purification protocols. Here we describe a very rapid, specific, sensitive, and reliable method for flavescence dorée phytoplasma detection, based on real-time Taqman(®) reverse transcription-PCR of the 16S rRNA. The protocol is particularly useful for large-scale screening of vineyards and nurseries, pathogen surveys, and field epidemiological studies.
authors.affiliation: Istituto di Virologia Vegetale, CNR, Torino, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Margaria
authors.forename: Sabrina
authors.initials: S
authors.lastname: Palmano
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D045582:Phytoplasma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S