title: Necropsy, blood, tissue collection, and mRNA isolation for detection of host cytokine gene expression.
abstract: Processing of tissue and blood must be done in a systematic and controlled fashion in order to optimize results and allow comparison of samples between experiments and between laboratories. Here we present our protocols for blood and tissue processing.
authors.affiliation: Unit for Laboratory Animal Medicine, Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Brian M
authors.initials: BM
authors.lastname: Gray
authors.forename: Kathryn A
authors.initials: KA
authors.lastname: Eaton
mesh_terms: D000818:Animals; D001344:Autopsy / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D001800:Blood Specimen Collection / Q000379:methods*; D046508:Culture Techniques; D016207:Cytokines / Q000235:genetics*; D005786:Gene Expression Regulation; D016480:Helicobacter pylori / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D051379:Mice; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013242:Sterilization
chemical_list: D016207:Cytokines; D012333:RNA, Messenger