title: [Cloning, expression and bioinformatics analysis of cyclophilin of Echinococcus granulosus].
abstract: OBJECTIVE
  To clone and express EgCyP gene of Echinococcus granulosas and analyze EgCyP using bioinformatics.
  
  
  METHODS
  Total RNAS of adult E. granulosus was extracted and reversedly transcripted to cDNA. EgCyP gene was amplified from cDNA and inserted into vector pET28a. Recombinant plasmid pET28a-EgCyP was transformed into E. coli BL21 (DE3) for expression under the induction of IPTG. The expressed product was identified by SDS-PAGE and Western blotting. EgCyP was analyzed by the bioinformatics software.
  
  
  RESULTS
  The EgCyP gene was successfully amplified from cDNA of adult E. granulosus and a fusion protein was expressed in E .coli BL21 (DE3). The molecular weight of the expressed protein was about 22 kDa. The Western blotting indicated that the antigenicity of the protein was specific. The bioinformatics analysis revealed that there were 7 antigen epitopes in EgCyP.
  
  
  CONCLUSIONS
  EgCyP of E. granulosus is cloned and expressed in E. coli BL21 (DE3) successfully, which might be the foundation for the further study of its immunogenicity.
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Laboratory of Parasite and Vector Biology, Ministry of Health, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Shen
authors.forename: Zhong-Ying
authors.initials: ZY
authors.lastname: Yuan
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Xu
authors.forename: Xiu-Min
authors.initials: XM
authors.lastname: Han
authors.forename: He-Jun
authors.initials: HJ
authors.lastname: Zhou
authors.forename: Jun-Yun
authors.initials: JY
authors.lastname: Wang
authors.forename: Hu
authors.initials: H
authors.lastname: Wang
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Cao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D019295:Computational Biology; D021983:Cyclophilins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D048209:Echinococcus granulosus / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D015870:Gene Expression; D008969:Molecular Sequence Data
chemical_list: D018076:DNA, Complementary; D000939:Epitopes; D021983:Cyclophilins