title: Identification of prohibitin as a Chikungunya virus receptor protein.
abstract: Chikungunya virus (CHIKV) has recently re-emerged causing millions of infections in countries around the Indian Ocean. While CHIKV has a broad host cell range and productively infects a number of different cell types, macrophages have been identified as a potential viral reservoir serving to increase the duration of symptoms. To date no CHIKV interacting protein has been characterized and this study sought to identify CHIKV binding proteins expressed on target cell membranes. Two-dimensional virus overlay identified prohibitin (PHB) as a microglial cell expressed CHIKV binding protein. Co-localization, co-immunoprecipitation as well as antibody and siRNA mediated infection inhibition studies all confirmed a role for PHB in mediating internalization of CHIKV into microglial cells. PHB is the first identified CHIKV receptor protein, and this study is evidence that PHB may play a role in the internalization of multiple viruses.
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Nakorn Pathom, Thailand.
authors.forename: Phitchayapak
authors.initials: P
authors.lastname: Wintachai
authors.forename: Nitwara
authors.initials: N
authors.lastname: Wikan
authors.forename: Atichat
authors.initials: A
authors.lastname: Kuadkitkan
authors.forename: Thitigun
authors.initials: T
authors.lastname: Jaimipuk
authors.forename: Sukathida
authors.initials: S
authors.lastname: Ubol
authors.forename: Rojjanaporn
authors.initials: R
authors.lastname: Pulmanausahakul
authors.forename: Prasert
authors.initials: P
authors.lastname: Auewarakul
authors.forename: Watchara
authors.initials: W
authors.lastname: Kasinrerk
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Weng
authors.forename: Mingkwan
authors.initials: M
authors.lastname: Panyasrivanit
authors.forename: Atchara
authors.initials: A
authors.lastname: Paemanee
authors.forename: Suthathip
authors.initials: S
authors.lastname: Kittisenachai
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.forename: Duncan R
authors.initials: DR
authors.lastname: Smith
mesh_terms: D000818:Animals; D002460:Cell Line; D002646:Chikungunya virus / Q000502:physiology*; D020868:Gene Silencing; D006801:Humans; D047468:Immunoprecipitation; D017628:Microglia / Q000737:chemistry / Q000821:virology; D009457:Neuroglia / Q000737:chemistry / Q000821:virology; D000091402:Prohibitins; D011991:Receptors, Virus / Q000378:metabolism*; D012097:Repressor Proteins / Q000378:metabolism*; D053585:Virus Attachment
chemical_list: C000717328:PHB protein, human; D000091402:Prohibitins; D011991:Receptors, Virus; D012097:Repressor Proteins