title: Proteomic features of potential tumor suppressor NESG1 in nasopharyngeal carcinoma.
abstract: We previously defined the recently revised NESG1 gene as a potential tumor suppressor in nasopharyngeal carcinoma (NPC). Here, we further used proteomics technology to globally examine NESG1-controlled proteins in NPC cells. Twenty-six proteins were found to be deregulated by NESG1 using proteomics analysis while enolase 1 (alpha) (ENO1), heat shock protein 90 kDa beta (Grp94), member 1 (HSP90B1), and cathepsin D (CTSD) proteins were differentially expressed by Western blot. Interestingly, a-enolase (ENO1), an overexpressed gene in NPC, was confirmed as a NESG1-regulated protein in NPC cells. Overexpressed ENO1 not only restored cell proliferation and cell-cycle progression, but also antagonized the regulation of NESG1 to cell-cycle regulators p21 and CCNA1 expression as well as induced the expression of C-Myc, pRB, and E2F1 in NESG1-ovexpressed NPC cells. Real-time PCR and immunohistochemistry analysis showed that NESG1 expression is negatively correlated with ENO1 expression in NPC tissues. Our observations suggest that ENO1 downregulation plays an important role in NESG1-induced growth inhibition of NPC cancer cells.
authors.affiliation: Department of Pathology, Basic School of Guangzhou Medical College, Guangzhou, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Huiling
authors.initials: H
authors.lastname: Yang
authors.forename: Yajie
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Long
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Long
authors.forename: Weiyi
authors.initials: W
authors.lastname: Fang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D060066:Protein Interaction Maps / Q000502:physiology; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D025521:Tumor Suppressor Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C121996:CFAP45 protein, human; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D011506:Proteins; D020543:Proteome; D025521:Tumor Suppressor Proteins; C095157:ENO1 protein, human; D010751:Phosphopyruvate Hydratase