title: Enhancement of viniferin production in Vitis vinifera L. cv. Alphonse Lavallée Cell suspensions by low-energy ultrasound alone and in combination with methyl jasmonate.
abstract: This study examined for the first time the effect of low-energy ultrasound (US), used alone or in combination with methyl jasmonate (MeJA), on viniferin production in cell cultures of Vitis vinifera L. cv Alphonse Lavallée. Cell suspensions were exposed for 2 min to US (power 30, 60, and 90 mW cm(-3)). The highest viniferin production was obtained at 30 mW cm(-3). When sonication was performed twice, the effect on viniferin production was negligible, whereas triple sonication slightly increased production. US treatment at 30 mW cm(-3) for 5 min decreased viniferin production and induced cellular death. The combined use of MeJA and US (2 min) increased the production of δ-viniferin, the dominant stilbene, more than each elicitor used alone. These results suggest that low-energy US, alone and in combination with MeJA, can act as a physical elicitor to stimulate viniferin production in V. vinifera cell cultures.
authors.affiliation: Department of Environmental Biology, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Santamaria
authors.forename: Marzia
authors.initials: M
authors.lastname: Innocenti
authors.forename: Nadia
authors.initials: N
authors.lastname: Mulinacci
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Melani
authors.forename: Alessio
authors.initials: A
authors.lastname: Valletta
authors.forename: Ilaria
authors.initials: I
authors.lastname: Sciandra
authors.forename: Gabriella
authors.initials: G
authors.lastname: Pasqua
mesh_terms: D000085:Acetates / Q000008:administration & dosage*; D001572:Benzofurans / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000008:administration & dosage*; D013058:Mass Spectrometry; D056004:Molecular Dynamics Simulation; D054883:Oxylipins / Q000008:administration & dosage*; D011789:Quantum Theory; D012118:Resorcinols / Q000378:metabolism*; D013267:Stilbenes / Q000378:metabolism*; D014465:Ultrasonics; D027843:Vitis / Q000166:cytology / Q000378:metabolism*
chemical_list: D000085:Acetates; D001572:Benzofurans; D003517:Cyclopentanes; D054883:Oxylipins; D012118:Resorcinols; D013267:Stilbenes; C477734:delta-viniferin; C072239:methyl jasmonate