title: Clinicopathologic significance of expression of nuclear factor-κB RelA and its target gene products in gastric cancer patients.
abstract: OBJECTIVE
  To assess the prognostic significance of nuclear factor-κB (NF-κB) and its target genes in gastric cancer.
  
  
  METHODS
  The tumor tissues of 115 patients with gastric cancer were immunohistochemically evaluated using monoclonal antibodies against NF-κB RelA. Preoperative serum levels of vascular endothelial growth factor (VEGF), interleukin-6 (IL-6) were assessed via enzyme-linked immuno-sorbent assay. C-reactive protein (CRP) and serum amyloid A (SAA) were measured via immunotrubidimetry.
  
  
  RESULTS
  Positive rate of NF-κB RelA was 42.6%. NF-κB RelA expression in tumor tissues was also related to serum levels of IL-6 (P = 0.044) and CRP (P = 0.010). IL-6, SAA, CRP were related to depth of invasion, VEGF and SAA were correlated with lymph node metastasis. IL-6, VEGF, SAA and CRP were related to the stage. Univariate analysis demonstrated that immunostaining of NF-κB RelA, levels of IL-6, VEGF, SAA were significantly related with both disease free survival and overall survival (OS). Multivariate analysis verified that NF-κB RelA [hazard ratio (HR): 3.40, P = 0.024] and SAA (HR: 3.39, P = 0.045) were independently associated with OS.
  
  
  CONCLUSIONS
  Increased expression of NF-κB RelA and high levels of serum SAA were associated with poor OS in gastric cancer patients.
authors.affiliation: Department of Internal Medicine, Dong-A University College of Medicine, Busan 602-715, South Korea.
authors.forename: Hyuk-Chan
authors.initials: HC
authors.lastname: Kwon
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Sung Yong
authors.initials: SY
authors.lastname: Oh
authors.forename: Suee
authors.initials: S
authors.lastname: Lee
authors.forename: Ji Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Jang
authors.forename: Min Chan
authors.initials: MC
authors.lastname: Kim
authors.forename: Ki Han
authors.initials: KH
authors.lastname: Kim
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Seong-Geun
authors.initials: SG
authors.lastname: Kim
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D002097:C-Reactive Protein / Q000032:analysis; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D000685:Serum Amyloid A Protein / Q000032:analysis; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology; D051996:Transcription Factor RelA / Q000032:analysis*; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis
chemical_list: D015850:Interleukin-6; C498143:RELA protein, human; D000685:Serum Amyloid A Protein; D051996:Transcription Factor RelA; D042461:Vascular Endothelial Growth Factor A; D002097:C-Reactive Protein