title: CPhos: a program to calculate and visualize evolutionarily conserved functional phosphorylation sites.
abstract: Profiling using high-throughput MS has discovered an overwhelming number of novel protein phosphorylation sites ("phosphosites"). However, the functional relevance of these sites is not always clear. In light of recent studies on the evolutionary mechanism of phosphorylation, we have developed CPhos, a Java program that can assess the conservation of phosphosites among species using an information theory-based approach. The degree of conservation established using CPhos can be used to assess the functional significance of phosphosites. CPhos has a user friendly graphical user interface and is available both as a web service and as a standalone Java application to assist phosphoproteomic researchers in analyzing and prioritizing lists of phosphosites for further experimental validation. CPhos can be accessed or downloaded at http://helixweb.nih.gov/CPhos/.
authors.affiliation: National Heart, Lung, and Blood Institute, Epithelial Systems Biology Laboratory, National Institutes of Health, Bethesda, MD 20892-1603, USA.
authors.forename: Boyang
authors.initials: B
authors.lastname: Zhao
authors.forename: Trairak
authors.initials: T
authors.lastname: Pisitkun
authors.forename: Jason D
authors.initials: JD
authors.lastname: Hoffert
authors.forename: Mark A
authors.initials: MA
authors.lastname: Knepper
authors.forename: Fahad
authors.initials: F
authors.lastname: Saeed
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D000818:Animals; D020134:Catalytic Domain; D019295:Computational Biology / Q000379:methods*; D017124:Conserved Sequence; D019143:Evolution, Molecular; D006801:Humans; D007257:Information Theory; D051379:Mice; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D051381:Rats; D015203:Reproducibility of Results; D020539:Sequence Analysis, Protein; D012984:Software; D014584:User-Computer Interface
chemical_list: D010750:Phosphoproteins