title: Effects of exogenous hydrogen sulfide on brain metabolism and early neurological function in rabbits after cardiac arrest.
abstract: OBJECTIVE
  Some of the neuroprotective effects of hydrogen sulfide (H(2)S) have been attributed to systemic hypometabolism and hypothermia. However, systemic metabolism may vary more dramatically than brain metabolism after cardiac arrest (CA). The authors investigated the effects of inhaled exogenous hydrogen sulfide on brain metabolism and neurological function in rabbits after CA and resuscitation.
  
  
  METHODS
  Anesthetized rabbits were randomized into a sham group, a sham/H(2)S group, a CA group, and a CA/H(2)S group. Exogenous 80 ppm H(2)S was administered to the sham/H(2)S group and the CA/H(2)S group which suffered 3 min of untreated CA by asphyxia and resuscitation. Effects on brain metabolism (cerebral extraction of oxygen (CEO(2)), arterio-jugular venous difference of glucose [AJVD(glu)] and lactate clearance), S100B, viable neuron counts, neurological dysfunction score, and survival rate were evaluated.
  
  
  RESULTS
  CEO(2), AJVD(glu), and lactate increased significantly after CA. Inhalation of 80 ppm H(2)S significantly increased CEO(2) (25.04 ± 7.11 vs. 16.72 ± 6.12 %) and decreased AJVD(glu) (0.77 ± 0.29 vs. 1.18 ± 0.38 mmol/L) and lactate (5.11 ± 0.43 vs. 6.01 ± 0.64 mmol/L) at 30 min after resuscitation when compared with the CA group (all P < 0.05). In addition, neurologic deficit scores, viable neuron counts, and survival rate were significantly better whereas S100B was decreased after H(2)S inhalation.
  
  
  CONCLUSIONS
  The present study reveals that inhalation of 80 ppm H(2)S reduced neurohistopathological damage and improves early neurological function after CA and resuscitation in rabbits. The increased CEO(2) and decreased AJVD(glu) and enhanced lactate clearance may be involved in the protective effects.
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical University, 150086, Harbin, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wei
authors.forename: Le
authors.initials: L
authors.lastname: Duan
authors.forename: Liqun
authors.initials: L
authors.lastname: Bai
authors.forename: Miaomiao
authors.initials: M
authors.lastname: Tian
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001831:Body Temperature; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002545:Brain Ischemia / Q000517:prevention & control*; D016887:Cardiopulmonary Resuscitation / Q000379:methods*; D006323:Heart Arrest / Q000188:drug therapy*; D006862:Hydrogen Sulfide / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D011817:Rabbits; D011897:Random Allocation; D016019:Survival Analysis
chemical_list: D018696:Neuroprotective Agents; D006862:Hydrogen Sulfide