title: Knockdown  expression of Apc11 leads to cell-cycle distribution reduction in G2/M phase.
abstract: Anaphase-promoting  complex/cyclosome (APC/C) is a key E3 ubiquitin ligase in cell division, which  catalyses ubiquitination of cell-cycle regulators. Studying this complex could  reveal important information regarding its application in cancer research and  therapy. In this study, 4 synthesized small interfering RNAs (siRNAs) were  transfected into HEK293T cells to suppress messenger RNA (mRNA) of Apc11; 2 of  these reduced the amount of Apc11 mRNA by over 50%. Further experiments showed  that rather than causing apoptosis, siRNA transfection led to cell-cycle  distributions characterized by less time spent in G2/M phase and more time  spent in G1 phase. This phenomenon was specifically induced by Apc11 silencing,  as co-transfection of siRNA and an Apc11 plasmid could reverse this  distribution bias. Our results suggested that siRNA targeted against Apc11  could hamper entry into G2/M phase. Current efforts are focused on elucidating the  function and utility of the APC complex for clinical applications.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, China.
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Shi
authors.forename: K-K
authors.initials: KK
authors.lastname: Huo
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome; D064198:Apc11 Subunit, Anaphase-Promoting Complex-Cyclosome; D002455:Cell Division; D016195:G2 Phase; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection; D043743:Ubiquitin-Protein Ligase Complexes / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D043743:Ubiquitin-Protein Ligase Complexes; C577351:ANAPC11 protein, human; D064173:Anaphase-Promoting Complex-Cyclosome; D064198:Apc11 Subunit, Anaphase-Promoting Complex-Cyclosome