title: [Cloning and eukaryotic expression of HIV-1B gp120 genes from a patient with AIDS dementia complex].
abstract: OBJECTIVE
  To clone and express the HIV-1B gp120 genes isolated at different organizations from a patient died of AIDS dementia complex (ADC) in eukaryotic cells.
  
  
  METHODS
  Using the genomic DNA isolated from peripheral lymphnodes, choroid plexus and occipital white matter from a patient died of ADC as the template, HIV-1B gp120 gene was amplified with PCR. After sequenced, HIV-1B gp120 was inserted into pcDNA3.1 (+) and recombinant expressing vector gp120/pcDNA3.1 (+) was constructed succeffuly confirming with sequencing. Then expressing vector was transfected into eukaryotic cells U87 using liposome transfection and expression of HIV-1B gp120 gene was assayed with indirect immunofluorescence.
  
  
  RESULTS
  HIV-1B gp120 genes isolated from peripheral lymphnodes, choroid plexus and occipital white matter of the ADC patient were successfully cloned and recombinant expressing vector gp120/pcDNA3; 1 (+) could express envelope glycoprotein HIV-1B gp120 in U87 cells.
  
  
  CONCLUSIONS
  All the HIV-1B gp120 gene isolated at the different organizations of the same ADC patient could express in U87 cells, which may supply a valuable basis for studying the neurotoxicity and neurotoxic mechanism of HIV-1 gp120 protein.
authors.affiliation: Department of Laboratory Science of Microbiology, Prevention and Health Care, Huangdao Branch, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266500, China. dlzhl@sdu.edu.cn
authors.forename: Li
authors.initials: L
authors.lastname: Zhao
authors.forename: Yu-Fen
authors.initials: YF
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Pu
authors.forename: Zhi-Yu
authors.initials: ZY
authors.lastname: Wang
authors.forename: Hong-Ling
authors.initials: HL
authors.lastname: Wen
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: Song
authors.forename: Hong-Zhi
authors.initials: HZ
authors.lastname: Xu
mesh_terms: D015526:AIDS Dementia Complex / Q000821:virology*; D003001:Cloning, Molecular; D015699:HIV Envelope Protein gp120 / Q000096:biosynthesis / Q000235:genetics* / Q000633:toxicity; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA
chemical_list: D015699:HIV Envelope Protein gp120; D011994:Recombinant Proteins; C514793:gp120 protein, Human immunodeficiency virus 1