title: Cruoricaptor ignavus gen. nov., sp. nov., a novel bacterium of the family Flavobacteriaceae isolated from blood culture of a man with bacteraemia.
abstract: A Gram-reaction-negative bacterium, strain IMMIB L-12475(T), was isolated from blood cultures of a human with septicaemia. The yellowish orange pigmented strain contained flexirubin pigment. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain IMMIB L-12475(T) belonged to the family Flavobacteriaceae, forming a distinct phyletic line that is distantly related (79.1-89.4% sequence similarity) to described genera of this family. Membership to the family was confirmed by a fatty acid profile consisting of branched-chain and 3-hydroxy fatty acids with major amounts of iso-C(17:0) 3-OH and iso-C(15:0), by the presence of menaquinone MK-6 as the only respiratory quinone and a polyamine pattern that contained sym-homospermidine as major component. The phospholipids consisted of phosphatidylethanolamine and an unknown phospholipid. The genomic DNA mol% G+C content was 45.6%. The distant phylogenetic position as compared to other representative of the family and the significant phenotypic properties such as pigment composition, morphology, and physiology support the proposal of a novel genus and species Cruoricaptor ignavus gen. nov., sp. nov. The type strain is IMMIB L-12475(T) (=DSM 25479(T)=CCUG 62025(T)).
authors.affiliation: Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, Sigmund-Freud-Straße 25, 53127 Bonn, Germany. yassin@mibi03.meb.uni-bonn.de
authors.forename: A F
authors.initials: AF
authors.lastname: Yassin
authors.forename: T J J
authors.initials: TJ
authors.lastname: Inglis
authors.forename: H
authors.initials: H
authors.lastname: Hupfer
authors.forename: C
authors.initials: C
authors.lastname: Siering
authors.forename: P
authors.initials: P
authors.lastname: Schumann
authors.forename: H-J
authors.initials: HJ
authors.lastname: Busse
authors.forename: M
authors.initials: M
authors.lastname: Aravena-Román
mesh_terms: D000328:Adult; D016470:Bacteremia / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D001769:Blood / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D045826:Flavobacteriaceae Infections / Q000382:microbiology*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011073:Polyamines / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011073:Polyamines; D011809:Quinones; D012336:RNA, Ribosomal, 16S