title: Multi walled carbon nano tubes induced hepatotoxicity in Swiss albino mice.
abstract: In the present study, multi walled carbon nano tubes (MWCNTs) were synthesized using chemical vapour deposition (CVD) technique. Swiss albino mice were orally administered with single dose of 60 and 100 mg/kg body weight of purified and functionalized MWCNTs suspended in water. The mice were autopsied on 7, 14, 21 and 28 days post exposure. Liver was taken out and part of it fixed in Bouin's solution for histopathological examinations. The remaining part was immersed in cold saline, blotted dry, weighed quickly and homogenized in ice cold buffer. The activity of superoxide dismutase (SOD) and catalase (CAT) was immediately measured in the supernatant. The MWCNTs in liver led to pathological changes, including injury to macrophages, cellular swelling, unspecific inflammation, spot necrosis and blood coagulation. Estimation of SOD and CAT showed altered levels in the experimental groups as compared to controls. Therefore, MWCNTs from manufactured and combustion sources in the environment can have adverse effects on human health.
authors.affiliation: Department of Zoology, University of Rajasthan, Jaipur (Rajasthan), India. kkantawasthi@gmail.com
authors.forename: Kumud Kant
authors.initials: KK
authors.lastname: Awasthi
authors.forename: P J
authors.initials: PJ
authors.lastname: John
authors.forename: Anjali
authors.initials: A
authors.lastname: Awasthi
authors.forename: Kamlendra
authors.initials: K
authors.lastname: Awasthi
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002374:Catalase / Q000032:analysis; D007249:Inflammation / Q000139:chemically induced; D008099:Liver / Q000187:drug effects* / Q000473:pathology*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D037742:Nanotubes, Carbon / Q000009:adverse effects*; D009336:Necrosis / Q000139:chemically induced; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D037742:Nanotubes, Carbon; D002374:Catalase; D013482:Superoxide Dismutase