title: Intronic RNAs constitute the major fraction of the non-coding RNA in mammalian cells.
abstract: BACKGROUND
  The function of RNA from the non-coding (the so called "dark matter") regions of the genome has been a subject of considerable recent debate. Perhaps the most controversy is regarding the function of RNAs found in introns of annotated transcripts, where most of the reads that map outside of exons are usually found. However, it has been reported that the levels of RNA in introns are minor relative to those of the corresponding exons, and that changes in the levels of intronic RNAs correlate tightly with that of adjacent exons. This would suggest that RNAs produced from the vast expanse of intronic space are just pieces of pre-mRNAs or excised introns en route to degradation.
  
  
  RESULTS
  We present data that challenges the notion that intronic RNAs are mere by-standers in the cell. By performing a highly quantitative RNAseq analysis of transcriptome changes during an inflammation time course, we show that intronic RNAs have a number of features that would be expected from functional, standalone RNA species. We show that there are thousands of introns in the mouse genome that generate RNAs whose overall abundance, which changes throughout the inflammation timecourse, and other properties suggest that they function in yet unknown ways.
  
  
  CONCLUSIONS
  So far, the focus of non-coding RNA discovery has shied away from intronic regions as those were believed to simply encode parts of pre-mRNAs. Results presented here suggest a very different situation--the sequences encoded in the introns appear to harbor a yet unexplored reservoir of novel, functional RNAs. As such, they should not be ignored in surveys of functional transcripts or other genomic studies.
authors.affiliation: Immunovirology-Biogenisis Group, University of Antioquia, Medellin A.A. 1226, Colombia. georgest98@yahoo.com
authors.forename: Georges
authors.initials: G
authors.lastname: St Laurent
authors.forename: Dmitry
authors.initials: D
authors.lastname: Shtokalo
authors.forename: Michael R
authors.initials: MR
authors.lastname: Tackett
authors.forename: Zhaoqing
authors.initials: Z
authors.lastname: Yang
authors.forename: Tatyana
authors.initials: T
authors.lastname: Eremina
authors.forename: Claes
authors.initials: C
authors.lastname: Wahlestedt
authors.forename: Silvio
authors.initials: S
authors.lastname: Urcuqui-Inchima
authors.forename: Bernd
authors.initials: B
authors.lastname: Seilheimer
authors.forename: Timothy A
authors.initials: TA
authors.lastname: McCaffrey
authors.forename: Philipp
authors.initials: P
authors.lastname: Kapranov
mesh_terms: D000818:Animals; D005091:Exons; D005260:Female; D005786:Gene Expression Regulation; D007438:Introns; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D008070:Lipopolysaccharides; D022661:RNA, Untranslated