title: Multiphoton imaging of freezing and heating effects in plant leaves.
abstract: Thermally-induced changes in Arabidopsis thaliana leaves were investigated with a novel cryo microscope by multiphoton, fluorescence lifetime and spectral imaging as well as micro spectroscopy. Samples were excited with fs pulses in the near-infrared range and cooled/heated in a cryogenic chamber. The results show morphological changes in the chloroplast distribution as well as a shift from chlorophyll to cell-wall fluorescence with decreasing temperature. At temperatures below -40 °C, also second harmonic generation was observed. The measurements illustrate the suitability of multiphoton imaging to investigate thermally-induced changes at temperatures used for cryopreservation as well as for basic investigations of thermal effects on plant tissue in general.
authors.affiliation: JenLab GmbH, Science Park 2, Campus D 1.2, 66123 Saarbrücken, Germany and Schillerstr. 1, 07745 Jena, Germany. breunig@jenlab.de
authors.forename: Hans Georg
authors.initials: HG
authors.lastname: Breunig
authors.forename: Fatma
authors.initials: F
authors.lastname: Tümer
authors.forename: Karsten
authors.initials: K
authors.lastname: König
mesh_terms: D017360:Arabidopsis / Q000166:cytology*; D005615:Freezing; D006358:Hot Temperature; D036641:Microscopy, Fluorescence, Multiphoton; D018515:Plant Leaves / Q000166:cytology*