title: [Difference of volatile constituents contained in female and male flowers of Trichosanthes kirilowii by HS-SPME-GC-MS].
abstract: OBJECTIVE
  To compare and analyze volatile constituents from flowers of Trichosanthes kirilowii, in order to point out characteristic differences between female and male flowers.
  
  
  METHODS
  Blooming female and male flowers were collected in the same place. Volatile constituents were extracted from the flower by solid phase micro-extraction (SPME), then separated and analyzed by gas chromatography-mass-spectrometry (GC-MS).
  
  
  RESULTS
  Fifty-two and forty-five chromatographic peaks were separated from the female and male flowers, respectively. Forty seven constituents were identified and their relative percentage compositions were determined with the peak area normalization method. Linalool, alpha-farnesene, benzene methanol, and (Z)-2-methylbutanal oxime were the main volatile constituents. The contents of linalool and alpha-farnesene in female flower were remarkably higher than those in male. In contrast, the content of benzene methanol in male flower was remarkably higher than that in female.
  
  
  CONCLUSIONS
  In the first study on chemical constituents from flowers in genus Trichosanthes, 37 compounds are separated from T. kirilowii. Contents of linalool, alpha-farnesene and benzene methanol show the characteristic differences of volatile constituents contained in male and female flowers of T. kirilowii, which enriches the basic studies on dioecious plant.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Sun
authors.forename: Zhimao
authors.initials: Z
authors.lastname: Chao
authors.forename: Chun
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wu
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Tan
mesh_terms: D035264:Flowers / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D052617:Solid Phase Microextraction; D029778:Trichosanthes / Q000737:chemistry*; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D055549:Volatile Organic Compounds