title: Nested PCR and RFLP analysis based on the 16S rRNA gene.
abstract: Current phytoplasma detection and identification methods are primarily based on nested polymerase chain reaction followed by restriction fragment length polymorphism analysis and gel electrophoresis. These methods can potentially detect and differentiate all phytoplasmas including those previously not described. The present protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels.
authors.affiliation: Laboratory of Applied Phytopathology, Institute of Pesticides and Environmental Protection, Belgrade, Serbia. bojan.duduk@pesting.org.rs
authors.forename: Bojan
authors.initials: B
authors.lastname: Duduk
authors.forename: Samanta
authors.initials: S
authors.lastname: Paltrinieri
authors.forename: Ing-Ming
authors.initials: IM
authors.lastname: Lee
authors.forename: Assunta
authors.initials: A
authors.lastname: Bertaccini
mesh_terms: D004269:DNA, Bacterial / Q000302:isolation & purification; D045582:Phytoplasma / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S