title: Inhibitory role of monovalent ions on rat brain cortex adenylyl cyclase activity.
abstract: Adenylyl cyclases, comprise of a large family of enzymes that catalyze synthesis of the cyclic AMP from ATP. The aim of our study was to determine the effect of monovalent ions on both basal, stimulated adenylate cyclase EC 4.6.1.1 (AC) activity and C unit of AC and on GTPase active G-protein in the synaptic membranes of rat brain cortex. The effect of ion concentration from 30 to 200 mM (1 mM MgCl2) showed dose-dependent and significant inhibition of the basal AC activity, stimulated and unstimulated C unit activity. Stimulation of AC with 5 μM GTPγS in the presence of 50-200 mM of tested salts showed inhibitory effect on the AC activity. From our results it could be postulated that the investigated monovalent ions exert inhibitory effect on the AC complex activity by affecting the intermolecular interaction of the activated α subunit of G/F protein and the C unit of AC complex an inhibitory influence of tested monovalent ions on these molecular interaction.
authors.affiliation: Department of Biochemistry, Faculty of medical sciences, University of Kragujevac , Kragujevac , Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Nikolic
authors.forename: Marina
authors.initials: M
authors.lastname: Mitrovic
authors.forename: Ivanka
authors.initials: I
authors.lastname: Zelen
authors.forename: Milan
authors.initials: M
authors.lastname: Zaric
authors.forename: Tatjana
authors.initials: T
authors.lastname: Kastratovic
authors.forename: Marijana
authors.initials: M
authors.lastname: Stanojevic
authors.forename: Milutin
authors.initials: M
authors.lastname: Nenadovic
authors.forename: Tomislav
authors.initials: T
authors.lastname: Stojanovic
mesh_terms: D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D007477:Ions / Q000737:chemistry / Q000494:pharmacology; D015636:Magnesium Chloride / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D004791:Enzyme Inhibitors; D007477:Ions; D015636:Magnesium Chloride; D000262:Adenylyl Cyclases