title: Analysis of protein expression in developmental toxicity induced by MeHg in zebrafish.
abstract: Mercury toxicity and its implications in development are a major concern, due to the major threat to ecosystems and human health that this compound represents. Although some of the effects of methylmercury (MeHg) exposure have been extensively studied, the molecular mechanisms of interaction between this compound and developing organisms are still not completely understood. To provide further insights into these mechanisms, we carried out a quantitative proteomic study (iTRAQ) using zebrafish larvae exposed to 5 μg L(-1) and 25 μg L(-1) MeHg as a model. In this study, a multidimensional approach combining isoelectric focusing (IEF) and strong cation exchange (SCX) followed by reversed phase liquid chromatography prior to MALDI TOF/TOF analysis was employed, which resulted in a substantial increase in proteome coverage. Among the proteins identified, 71 were found de-regulated by more than 1.5-fold, and implicated in embryonic development, protein synthesis, calcium homeostasis and energy production. Furthermore, morphological and histological analysis of exposed larvae was carried out, reflecting changes such as smaller swim bladder, remaining yolk, bent body axis and accumulation of blood in the heart, among others.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040 Madrid, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Cuello
authors.forename: Pilar
authors.initials: P
authors.lastname: Ximénez-Embún
authors.forename: Isabel
authors.initials: I
authors.lastname: Ruppen
authors.forename: Helia B
authors.initials: HB
authors.lastname: Schonthaler
authors.forename: Keith
authors.initials: K
authors.lastname: Ashman
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Madrid
authors.forename: Jose L
authors.initials: JL
authors.lastname: Luque-Garcia
authors.forename: Carmen
authors.initials: C
authors.lastname: Cámara
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D056148:Chromatography, Reverse-Phase; D047108:Embryonic Development / Q000187:drug effects*; D004734:Energy Metabolism; D007525:Isoelectric Focusing; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008767:Methylmercury Compounds / Q000737:chemistry / Q000633:toxicity*; D010455:Peptides / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015027:Zebrafish / Q000254:growth & development
chemical_list: D008767:Methylmercury Compounds; D010455:Peptides; D020543:Proteome; D002118:Calcium