title: Choline-containing phospholipids in microdissected human Alzheimer's disease brain senile plaque versus neuropil.
abstract: BACKGROUND
  Lipidomic studies related to Alzheimer's disease have been reported on either biological fluids or large human brain samples. For a better understanding of the role of lipids, especially during the amyloid-β peptide aggregation, it is crucial to determine the composition of the senile plaque versus the surrounding tissue, that is, the neuropil.
  
  
  RESULTS
  A laser microdissection step was added to the analysis by UPLC-MS/MS. Despite the very low amount of sample, two phosphatidylcholines that were significantly depleted in the senile plaque were identified.
  
  
  CONCLUSIONS
  Changes in the phospholipid content have been shown between senile plaque versus neuropil. Nano HPLC, allowing a complete lipidomic profile, should further improve the results.
authors.affiliation: Chimie-Toxicologie Analytique et Cellulaire, EA 4463, Faculté des Sciences Pharmaceutiques et Biologiques, Université Paris Descartes, PRES Sorbonne Paris Cité, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Gaudin
authors.forename: Maï
authors.initials: M
authors.lastname: Panchal
authors.forename: Nicolas
authors.initials: N
authors.lastname: Auzeil
authors.forename: Charles
authors.initials: C
authors.lastname: Duyckaerts
authors.forename: Alain
authors.initials: A
authors.lastname: Brunelle
authors.forename: Olivier
authors.initials: O
authors.lastname: Laprévote
authors.forename: David
authors.initials: D
authors.lastname: Touboul
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001923:Brain Chemistry; D002794:Choline / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D042282:Microdissection; D019581:Neuropil / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*; D058225:Plaque, Amyloid / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
chemical_list: D010743:Phospholipids; D002794:Choline