title: The DUBm subunit Sgf11 is required for mRNA export and interacts with Cbp80 in Drosophila.
abstract: SAGA/TFTC is a histone acetyltransferase complex that has a second enzymatic activity because of the presence of a deubiquitination module (DUBm). Drosophila DUBm consists of Sgf11, ENY2 and Nonstop proteins. We show that Sgf11 has other DUBm-independent functions. It associates with Cbp80 component of the cap-binding complex and is thereby recruited onto growing messenger ribonucleic acid (mRNA); it also interacts with the AMEX mRNA export complex and is essential for hsp70 mRNA export, as well as for general mRNA export from the nucleus. Thus, Sgf11 functions as a component of both SAGA DUBm and the mRNA biogenesis machinery.
authors.affiliation: Department of Regulation of Gene Expression, Institute of Gene Biology, Russian Academy of Sciences, Vavilov Street 32, Moscow 119991, Russia.
authors.forename: Dmitriy
authors.initials: D
authors.lastname: Gurskiy
authors.forename: Anastasija
authors.initials: A
authors.lastname: Orlova
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Vorobyeva
authors.forename: Elena
authors.initials: E
authors.lastname: Nabirochkina
authors.forename: Alexey
authors.initials: A
authors.lastname: Krasnov
authors.forename: Yulii
authors.initials: Y
authors.lastname: Shidlovskii
authors.forename: Sofia
authors.initials: S
authors.lastname: Georgieva
authors.forename: Daria
authors.initials: D
authors.lastname: Kopytova
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004330:Drosophila / Q000196:embryology / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D004625:Embryo, Nonmammalian / Q000378:metabolism; D055785:Gene Knockdown Techniques; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response; D051548:Histone Acetyltransferases / Q000737:chemistry; D039502:Nuclear Cap-Binding Protein Complex / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D029543:Nucleocytoplasmic Transport Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D021122:Protein Subunits / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D014157:Transcription Factors / Q000032:analysis / Q000378:metabolism* / Q000502:physiology; D015533:Transcriptional Activation
chemical_list: C577837:Cbp80 protein, Drosophila; D029721:Drosophila Proteins; D018840:HSP70 Heat-Shock Proteins; D039502:Nuclear Cap-Binding Protein Complex; D029543:Nucleocytoplasmic Transport Proteins; D021122:Protein Subunits; D012333:RNA, Messenger; C527172:Sgf11 protein, Drosophila; D014157:Transcription Factors; D051548:Histone Acetyltransferases