title: [Immunophenotyping in adult acute myeloid leukemia: which prognostic value?].
abstract: BACKGROUND
  Immunophenotyping is an essential step in the diagnosis of acute myeloid leukemia. Its prognostic value remains controversial with contradictory results.
  
  
  OBJECTIVE
  To assess prognostic impact of the immunophenotyping in AML.
  
  
  METHODS
  Our study is retrospective (October, 2005 - July, 2007) concerning 56 cases of AML (AML3 excluded) of the adult from 18 to 55 years old diagnosed and treated in Tunis Aziza Othmana Hospital. The immunophenotyping was performed by flow cytometry (Beckman Coulter EPICS XL MCL®). We studied clinical and biological characteristic, immunophenotypic expressions, and parameters of the response to the treatment: complete remission (CR), overall survival (OS), relapse free survival (RFS) and relapse in a delay of 1 year and 2 years. SPSS software was used to perform the statistical analysis.
  
  
  RESULTS
  The median age of the patients is of 37,7±11.8 years. Sex ratio (M/F) is 1.33. Among individual antigenic expressions, only CD7 is associated to lower CR rates (p=0.044). We did not find any statistically significant association between immunophenotypic expressions and OS nor with relapse or RFS.
  
  
  CONCLUSIONS
  The impact of immunophenotyping in AML remains controversial because of contradictory results. The research of molecular changes would be an interesting alternative in our context.
authors.affiliation: Dipartement d'Hematologie, Universite Tunis, El Manar, Tunisie.
authors.forename: Naouel
authors.initials: N
authors.lastname: Ben Salah
authors.forename: Emna
authors.initials: E
authors.lastname: Gouider
authors.forename: Hajer
authors.initials: H
authors.lastname: Aounallah Skhiri
authors.forename: Wijdene
authors.initials: W
authors.lastname: El Borgi
authors.forename: Moenes
authors.initials: M
authors.lastname: Jouabli
authors.forename: Fatma
authors.initials: F
authors.lastname: Ben Lakhal
authors.forename: Ramzi
authors.initials: R
authors.lastname: Jeddi
authors.forename: Raouf
authors.initials: R
authors.lastname: Hafsia
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000276:immunology* / Q000401:mortality / Q000628:therapy; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012074:Remission Induction; D012189:Retrospective Studies; D015996:Survival Rate; D055815:Young Adult