title: Cellular dewetting: opening of macroapertures in endothelial cells.
abstract: Pathogenic bacteria can cross from blood vessels to host tissues by opening transendothelial cell macroapertures (TEMs). To induce TEM opening, bacteria intoxicate endothelial cells with proteins that disrupt the contractile cytoskeletal network. Cell membrane tension is no longer resisted by contractile fibers, leading to the opening of TEMs. Here we model the opening of TEMs as a new form of dewetting. While liquid dewetting is irreversible, we show that cellular dewetting is transient. Our model predicts the minimum radius for hole nucleation, the maximum TEM size, and the dynamics of TEM opening, in good agreement with experimental data. The physical model is then coupled with biological experimental data to reveal that the protein missing in metastasis (MIM) controls the line tension at the rim of the TEM and opposes its opening.
authors.affiliation: Institut Curie, CNRS UMR 168 and Université Paris 6, Paris, France. davidgr@alum.mit.edu
authors.forename: David
authors.initials: D
authors.lastname: Gonzalez-Rodriguez
authors.forename: Madhavi P
authors.initials: MP
authors.lastname: Maddugoda
authors.forename: Caroline
authors.initials: C
authors.lastname: Stefani
authors.forename: Sebastien
authors.initials: S
authors.lastname: Janel
authors.forename: Frank
authors.initials: F
authors.lastname: Lafont
authors.forename: Damien
authors.initials: D
authors.lastname: Cuvelier
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Lemichez
authors.forename: Françoise
authors.initials: F
authors.lastname: Brochard-Wyart
mesh_terms: D001426:Bacterial Proteins / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D061307:Human Umbilical Vein Endothelial Cells / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology / Q000648:ultrastructure*; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008954:Models, Biological; D011994:Recombinant Proteins / Q000494:pharmacology; D017155:Wettability
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; C067120:epidermal cell differentiation inhibitor, Staphylococcus aureus