title: YgiW homologous gene from Pseudomonas aeruginosa 25W is responsible for tributyltin resistance.
abstract: A tributyltin (TBT) resistance gene was isolated from the TBT-resistant marine origin bacterium Pseudomonas aeruginosa 25W. This gene was identical to PA0320 deposited in the P. aeruginosa PAO1 database (http://www.pseudomonas.com). The deduced amino acid sequence of PA0320 appears to be homologous to the YgiW proteins of Escherichia coli and Salmonella enterica. The deletion mutant of PA0320 showed a reduction of growth rate in the presence of TBT. A susceptibility test to cadmium, mercury, hydrogen peroxide and acidic pH in the deletion mutant showed an increasing susceptibility to them. PA0320 plays a certain role in stress tolerance against TBT as well as in stressors producing reactive oxygen species.
authors.affiliation: Center for Marine Environmental Studies (CMES), Ehime University, Matsuyama, Ehime 790-8577, Japan.
authors.forename: Koh
authors.initials: K
authors.lastname: Fukushima
authors.forename: Santosh Dubey
authors.initials: SD
authors.lastname: Kumar
authors.forename: Satoru
authors.initials: S
authors.lastname: Suzuki
mesh_terms: D000143:Acids / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002104:Cadmium / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008628:Mercury / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D019779:Salmonella enterica / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D014220:Trialkyltin Compounds / Q000494:pharmacology*
chemical_list: D000143:Acids; D000900:Anti-Bacterial Agents; D014220:Trialkyltin Compounds; D002104:Cadmium; C011559:tributyltin; D006861:Hydrogen Peroxide; D008628:Mercury