title: Photosystem I in Langmuir-Blodgett and Langmuir-Schaefer monolayers.
abstract: Photosystem I (PSI) is a membrane protein complex that generates photoinduced electrons and transfers them across the thylakoid membrane during photosynthesis. The PSI complex, separated from spinach leaves, was spread onto the air-water interface as a monolayer and transferred onto a gold electrode surface that was precoated with a self-assembled monolayer (SAM). The electrochemical properties of the transferred PSI monolayer, including cyclic voltammetry and photoinduced chronoamperometry, were measured. The results showed that PSI retained its bioactivity after the manipulation. Its capability of converting photoenergy into electrical potential was demonstrated by its reducing an electron acceptor, dichloroindophenol (DCIP), and by oxidizing an electron donor, sodium ascorbate (ASC). We have shown that the protein has two possible orientations at the water interface. The orientation distribution was determined by comparing the controlled reductive and oxidative photocurrents generated from Langmuir-Blodgett and Langmuir-Schaefer monolayers.
authors.affiliation: Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235-1822, United States.
authors.forename: Xun
authors.initials: X
authors.lastname: Yan
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Faulkner
authors.forename: G Kane
authors.initials: GK
authors.lastname: Jennings
authors.forename: David E
authors.initials: DE
authors.lastname: Cliffel
mesh_terms: D015086:2,6-Dichloroindophenol / Q000737:chemistry; D000388:Air; D001205:Ascorbic Acid / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D045331:Photosystem I Protein Complex / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D045331:Photosystem I Protein Complex; D014867:Water; D006046:Gold; D015086:2,6-Dichloroindophenol; D001205:Ascorbic Acid