title: Isolation and characterization of bacterial isolates algicidal against a harmful bloom-forming cyanobacterium Microcystis aeruginosa.
abstract: Algicidal bacteria MaI11-2, MaI11-5 and MaI11-10, which inhibited the growth of a harmful bloom-forming cyanobacterium Microcystis aeruginosa, were isolated from a sewage treatment plant. The isolate MaI11-5 was phylogenetically affiliated into the genus Pedobacter, while MaI11-2 and MaI11-10 were closely related to Bacillus aerophilus, Bacillus altitudinis and Bacillus stratosphericus with 100% identity based on 16S ribosomal RNA sequences. Co-cultivation of M. aeruginosa with the algicidal isolates showed their high algicidal activity. MaI11-5 showed the highest inhibitory effect on the cyanobacterial growth: the inhibitory effect exceeded 50% after 2 days, and reached to 75-85% after 10 days, regardless of the bacterial cell density. The cyanobacterial cells aggregated and produced mucilaginous, glycocalyx-like compounds when attacked by the algicidal bacteria. These results suggest that the algicidal bacteria isolated in the present study are potentially useful as biocontrol agents against M. aeruginosa bloom.
authors.affiliation: Department of Environmental Engineering, College of Environment, Hohai University, Nanjing 210098, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Hongyi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Komatsu
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Lin
authors.initials: L
authors.lastname: Jinsan
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Ito
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshikawa
authors.forename: Hiroto
authors.initials: H
authors.lastname: Maeda
mesh_terms: D000898:Antibiosis; D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D046931:Microcystis / Q000254:growth & development* / Q000502:physiology; D008969:Molecular Sequence Data; D060485:Pedobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology*
chemical_list: D012722:Sewage