title: Colon neoplastic cells do not originate from bone marrow-derived cells after sex-mismatched bone marrow transplantation.
abstract: BACKGROUND
  Although previous studies indicate that gastrointestinal (GI) cancer may originate from cells recruited from bone marrow (BM) in mice, whether similar phenomena occur in humans is controversial. In the current study, we evaluated two female patients who developed colonic adenocarcinoma more than 10 years after gender-mismatched BM transplantation, and followingly underwent successful endoscopic mucosal resection.
  
  
  METHODS
  Fluorescent in situ hybridization (FISH) analysis was used to determine whether the tumours contained donor-derived BM cells.
  
  
  RESULTS
  Approximately 1.2% of the tumour cells contained Y-chromosome-positive signals, and a comparable percentage of normal colonic epithelial cells close to the tumour also contained Y-chromosome-positive signals.
  
  
  CONCLUSIONS
  These results do not support the concept that GI cancer can originate from BM-derived cells.
authors.affiliation: Division of Gastroenterology and Hepatology, Keio University School of Medicine, Tokyo, Japan.
authors.forename: Gen
authors.initials: G
authors.lastname: Sakai
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Yajima
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Takaishi
authors.forename: Takehiko
authors.initials: T
authors.lastname: Mori
authors.forename: Hajime
authors.initials: H
authors.lastname: Higuchi
authors.forename: Shoko
authors.initials: S
authors.lastname: Nakamura
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Funakoshi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Adachi
authors.forename: Motoko
authors.initials: M
authors.lastname: Izumiya
authors.forename: Hideko
authors.initials: H
authors.lastname: Akagi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Hamamoto
authors.forename: Takanori
authors.initials: T
authors.lastname: Kanai
authors.forename: Makio
authors.initials: M
authors.lastname: Mukai
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Okamoto
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Hibi
mesh_terms: D000230:Adenocarcinoma / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D000328:Adult; D001854:Bone Marrow Cells / Q000473:pathology* / Q000648:ultrastructure; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000473:pathology*; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D003110:Colonic Neoplasms / Q000209:etiology / Q000235:genetics / Q000473:pathology*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000473:pathology* / Q000648:ultrastructure; D012737:Sex Factors