title: Acupuncture at SI5 attenuates morphine seeking behavior after extinction.
abstract: Our previous studies have shown that acupuncture attenuates morphine self-administration and sensitization behavior as well as withdrawal signs. The present study was designed to investigate the role of acupuncture in the reinstatement of morphine seeking. Male Sprague-Dawley rats weighing 270-300 g were subjected to intravenous catheterization after food training. The animals were trained to self-administer morphine (1.0mg/kg, 3 weeks), followed by extinction (1 week). Extinction conditions were introduced by substituting saline for morphine. The rats were then tested for reinstatement of morphine self-administration by a priming injection of morphine (0.25mg/kg). To see whether acupuncture can reduce morphine reinstatement, acupuncture was performed at SI5 or LI5 for 1 min immediately before a morphine injection. To further test the involvement of gamma aminobutyric acid (GABA) receptors in acupuncture effects, GABA receptor antagonists were injected before acupuncture. In the present results, acupuncture at SI5, but not at control acupoint LI5 attenuated the reinstatement of morphine seeking behavior, which was blocked by the GABA receptor antagonists. It suggests that acupuncture can reduce the reinstatement of morphine seeking, possibly due to the mediation of GABA receptor system.
authors.affiliation: Department of Acupuncture, Moxibustion and Acupoint, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea. dlqhdgy@dhu.ac.kr
authors.forename: Bong Hyo
authors.initials: BH
authors.lastname: Lee
authors.forename: Jeong Hun
authors.initials: JH
authors.lastname: Ma
authors.forename: Sunghyun
authors.initials: S
authors.lastname: In
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Seong Shoon
authors.initials: SS
authors.lastname: Yoon
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Jang
authors.forename: Chae Ha
authors.initials: CH
authors.lastname: Yang
mesh_terms: D015670:Acupuncture Therapy / Q000379:methods*; D000818:Animals; D016739:Behavior, Addictive / Q000503:physiopathology / Q000517:prevention & control; D001522:Behavior, Animal / Q000187:drug effects*; D005108:Extinction, Psychological / Q000187:drug effects*; D008297:Male; D009020:Morphine / Q000633:toxicity*; D009021:Morphine Dependence / Q000503:physiopathology* / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012646:Self Administration; D016896:Treatment Outcome
chemical_list: D009020:Morphine