title: Consistent divergence times and allele sharing measured from cross-species application of SNP chips developed for three domestic species.
abstract: Recent advances in technology facilitated development of large sets of genetic markers for many taxa, though most often model or domestic organisms. Cross-species application of genomic technologies may allow for rapid marker discovery in wild relatives of taxa with well-developed resources. We investigated returns from cross-species application of three commercially available SNP chips (the OvineSNP50, BovineSNP50 and EquineSNP50 BeadChips) as a function of divergence time between the domestic source species and wild target species. Across all three chips, we observed a consistent linear decrease in call rate (~1.5% per million years), while retention of polymorphisms showed an exponential decay. These results will allow researchers to predict the expected amplification rate and polymorphism of cross-species application for their taxa of interest, as well as provide a resource for estimating divergence times.
authors.affiliation: Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada. jmm1@ualberta.ca
authors.forename: J M
authors.initials: JM
authors.lastname: Miller
authors.forename: J W
authors.initials: JW
authors.lastname: Kijas
authors.forename: M P
authors.initials: MP
authors.lastname: Heaton
authors.forename: J C
authors.initials: JC
authors.lastname: McEwan
authors.forename: D W
authors.initials: DW
authors.lastname: Coltman
mesh_terms: D000483:Alleles; D000818:Animals; D000829:Animals, Domestic / Q000145:classification* / Q000235:genetics*; D005838:Genotype; D046228:Microarray Analysis / Q000379:methods*; D008967:Molecular Biology / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide