title: Antitumor actions of baicalein and wogonin in HT-29 human colorectal             cancer cells.
abstract: The purpose of this study was to determine the effects of baicalein and             wogonin, which are compounds derived from the Chinese herb Scutellaria baicalensis,             in suppressing the viability of HT-29 human colon cancer cells. Following treatment             with baicalein or wogonin, several apoptotic events were observed, including DNA             fragmentation, chromatin condensation and increased cell cycle arrest in the G1             phase. Baicalein and wogonin decreased Bcl-2 expression, whereas the expression             of Bax was increased in a dose-dependent manner compared with the control. Furthermore,             the induction of apoptosis was accompanied by an inactivation of phosphatidylinositol             3-kinase (PI3K)/Akt in a dose-dependent manner. The administration of baicalein             to mice resulted in the inhibition of the growth of HT-29 xenografts without any             toxicity following 5 weeks of treatment. The results indicated that baicalein             induced apoptosis via Akt activation in a p53-dependent manner in the HT-29 colon             cancer cells and that it may serve as a chemopreventive or therapeutic agent for             HT-29 colon cancer.
authors.affiliation: Department of Companion and Laboratory Animal Science, Kongju                     National University, Yesan 340-702, Republic of Korea.
authors.forename: So-Jung
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hyeong-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Hye-Ri
authors.initials: HR
authors.lastname: Kim
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Sung-Dae
authors.initials: SD
authors.lastname: Cho
authors.forename: Chang-Sun
authors.initials: CS
authors.lastname: Choi
authors.forename: Jeong-Seok
authors.initials: JS
authors.lastname: Nam
authors.forename: Ji-Youn
authors.initials: JY
authors.lastname: Jung
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D004249:DNA Damage / Q000187:drug effects*; D044950:Flavanones / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D031363:Scutellaria / Q000737:chemistry; D014183:Transplantation, Heterologous; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C006680:baicalein; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C085514:wogonin