title: Ex vivo culture of Plasmodium vivax and Plasmodium cynomolgi and in vitro culture of Plasmodium knowlesi blood stages.
abstract: Long-term in vitro cultures of blood-stage parasites are so far feasible only for Plasmodium falciparum and P. knowlesi. In this chapter, we describe short-term ex vivo culturing of P. cynomolgi and P. vivax. We also describe long-term in vitro culturing of P. knowlesi as well as some techniques for synchronizing parasites. Cultured parasites can be used for a variety of purposes, e.g., for in vitro drug assays and antibody-mediated growth inhibition assays.
authors.affiliation: Biomedical Primate Research Centre, Rijswijk, The Netherlands.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Zeeman
authors.forename: Annemarie Voorberg-van
authors.initials: AV
authors.lastname: der Wel
authors.forename: Clemens H M
authors.initials: CH
authors.lastname: Kocken
mesh_terms: D000409:Alanine / Q000494:pharmacology; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D001399:Azure Stains; D018929:Cell Culture Techniques / Q000379:methods*; D015925:Cryopreservation / Q000379:methods; D004912:Erythrocytes / Q000469:parasitology*; D006801:Humans; D008018:Life Cycle Stages / Q000187:drug effects; D008253:Macaca mulatta / Q000469:parasitology; D021261:Parasitic Sensitivity Tests; D016789:Plasmodium cynomolgi / Q000187:drug effects / Q000254:growth & development*; D016790:Plasmodium knowlesi / Q000187:drug effects / Q000254:growth & development*; D010966:Plasmodium vivax / Q000187:drug effects / Q000254:growth & development*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000962:Antimalarials; D001399:Azure Stains; D000409:Alanine