title: [Changes of neuroglobin in the pathologic process of contusion and laceration of brain in children].
abstract: OBJECTIVE
  To study the role of neuroglobin (Ngb) in the pathologic process of contusion and laceration of brain in children.
  
  
  METHODS
  The proteins in the brain tissue were extracted by two-dimensional gel electrophoresis in 3 children undergoing brain ventricular neoplasms resection (normal brain tissue) and in 8 children with contusion and laceration of brain. The image analysis was done using the PDQuest 7.0 software. The differential protein spots were detected and analyzed with Applied Biosystems Voyager System 4307 MALDI-TOF Mass Spectrometer and bioinformatical skills. Ngb expression in the brain tissue was measured using immunohistochemisty. Ngb expression in plasma was measured using ELISA in 15 children with contusion and laceration of brain and 10 healthy children.
  
  
  RESULTS
  Expression maps of the brain tissue were established by two-dimensional gel electrophoresis in children with contusion and laceration of brain and healthy children. Six differential protein spots were found and 5 of them were identified by mass spectrum. Immunohistochemisty assay showed that Ngb expression in the brain tissue in children with contusion and laceration of brain was significantly higher than in normal controls (P<0.05). ELISA results showed that Ngb expression in the plasma increased significantly 6, 12, 18, 24 and 48 hours after trauma in children with contusion and laceration of brain compared with healthy children (P<0.01).
  
  
  CONCLUSIONS
  Ngb may play an important role in the pathologic process of contusion and laceration of brain in children.
authors.affiliation: Department of Neurosurgery, Xaingya Hospital of Central South University, Changsha, China.
authors.forename: Zhen-Yan
authors.initials: ZY
authors.lastname: Li
authors.forename: Zheng-Hao
authors.initials: ZH
authors.lastname: Deng
authors.forename: Chun-Tao
authors.initials: CT
authors.lastname: Li
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Tang
authors.forename: Guang-Wei
authors.initials: GW
authors.lastname: Zhong
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Yun-Sheng
authors.initials: YS
authors.lastname: Liu
authors.forename: Jing-Ping
authors.initials: JP
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D001930:Brain Injuries / Q000378:metabolism*; D002648:Child; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005914:Globins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D000078787:Neuroglobin; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D005914:Globins