title: Tracing fatty acid metabolism by click chemistry.
abstract: Fatty acids are abundant constituents of all biological systems, and their metabolism is important for normal function at all levels of an organism. Aberrations in fatty acid metabolism are associated with pathological states and have become a focus of current research, particularly due to the interest in metabolic overload diseases. Here we present a click-chemistry-based method that allows tracing of fatty acid metabolism in virtually any biological system. It combines high sensitivity with excellent linearity and fast sample turnover. Since it is free of radioactivity, it can be combined with any other modern analysis technology and can be used in high-throughput applications. Using the new method, we provide for the first time an analysis of cellular fatty metabolism with high time resolution and a comprehensive comparison of utilization of a broad spectrum of fatty acids in hepatoma and adipose cell lines.
authors.affiliation: LIMES Life and Medical Sciences Institute, University of Bonn, Carl-Troll-Strasse 31, D-53115 Bonn, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Thiele
authors.forename: Cyrus
authors.initials: C
authors.lastname: Papan
authors.forename: Dominik
authors.initials: D
authors.lastname: Hoelper
authors.forename: Kalina
authors.initials: K
authors.lastname: Kusserow
authors.forename: Anne
authors.initials: A
authors.lastname: Gaebler
authors.forename: Mario
authors.initials: M
authors.lastname: Schoene
authors.forename: Kira
authors.initials: K
authors.lastname: Piotrowitz
authors.forename: Daniel
authors.initials: D
authors.lastname: Lohmann
authors.forename: Johanna
authors.initials: J
authors.lastname: Spandl
authors.forename: Ana
authors.initials: A
authors.lastname: Stevanovic
authors.forename: Andrej
authors.initials: A
authors.lastname: Shevchenko
authors.forename: Lars
authors.initials: L
authors.lastname: Kuerschner
mesh_terms: D000818:Animals; D002460:Cell Line; D002855:Chromatography, Thin Layer; D004330:Drosophila; D005227:Fatty Acids / Q000378:metabolism*; D006801:Humans
chemical_list: D005227:Fatty Acids