title: Sulfonyl fluoride analogues as activity-based probes for serine proteases.
abstract: Enriched with fluoride: To expand on the available tools to interrogate proteases, we explored sulfonyl fluorides as activity-based probes. An alkyne-tagged sulfonyl fluoride covalently modifies members of the S1 family of serine proteases. By applying click chemistry, avidin enrichment and mass spectrometry, we can enrich and identify active endogenous serine proteases from a complex proteome.
authors.affiliation: Department of Chemistry, Boston College, Chestnut Hill, MA 02467, USA.
authors.forename: D Alexander
authors.initials: DA
authors.lastname: Shannon
authors.forename: Christian
authors.initials: C
authors.lastname: Gu
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: McLaughlin
authors.forename: Markus
authors.initials: M
authors.lastname: Kaiser
authors.forename: Renier A L
authors.initials: RA
authors.lastname: van der Hoorn
authors.forename: Eranthie
authors.initials: E
authors.lastname: Weerapana
mesh_terms: D015335:Molecular Probes / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D057057:Serine Proteases / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013441:Sulfinic Acids / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D015335:Molecular Probes; D015842:Serine Proteinase Inhibitors; D013441:Sulfinic Acids; C048899:sulfuryl fluoride; D057057:Serine Proteases