title: [Impact of saikosaponin on absorption and transport of paeoniflorin in Caco-2 cell model].
abstract: OBJECTIVE
  To study the impact of saikosaponin on absorption and transport of paeoniflorin in Caco-2 cell model.
  
  
  METHODS
  The concentration of paeoniflorin in cell culture medium was measured by UPLC and the apparent permeability coefficients (P(app)) was calculated to study differences in bi-direction transport of paeoniflorin solutions of different concentrations and its compatibility with saikosaponin a, and saikosaponin d in Caco-2 cell model. Meanwhile, the electric resistance of Caco-2 cell was determined before and after the experiment.
  
  
  RESULTS
  The amount of paeoniflorin increased linearly with the transport of Caco-2 cell monolayer in 4 h, with a lower absorptive permeability, which was (0.98 +/- 0.10) x 10(-6), (0.92 +/- 0.09) x 10(-6), (0.89 +/- 0.04) x 10(-6) cm x s(-1) at the concentration of 20, 50, 100 micromol x L(-1), respectively. After compatibility with saikosaponin a and saikosaponin d, the absorptive permeability of paeoniflorin increased by 2.37 times and 2.54 times, respectively, while the electric resistance of Caco-2 cell was decreased significantly after the experiment.
  
  
  CONCLUSIONS
  Saikosaponin a, d can enhance the absorption of paeoniflorin in Caco-2 cell monolayer, which may be related to saikosaponin's ability to open up intercellular tight junctions among Caco-2 cells.
authors.affiliation: Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, State Administration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China. ychen202@yahoo.com.cn
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Jinyan
authors.initials: J
authors.lastname: Wang
authors.forename: Ran
authors.initials: R
authors.lastname: Xin
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Tan
mesh_terms: D000042:Absorption / Q000187:drug effects; D001565:Benzoates / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D001952:Bridged-Ring Compounds / Q000378:metabolism*; D018938:Caco-2 Cells; D004305:Dose-Response Relationship, Drug; D055724:Electrophysiological Phenomena / Q000187:drug effects; D005960:Glucosides / Q000378:metabolism*; D006801:Humans; D039821:Monoterpenes; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D005960:Glucosides; D039821:Monoterpenes; D009828:Oleanolic Acid; D012503:Saponins; C015423:peoniflorin; D006027:Glycosides; C025759:saikosaponin D