title: Development of a bioassay system for human growth hormone determination with close correlation to immunoassay.
abstract: Serum growth hormone (GH) level is measured largely through immunoassays in clinical practice. However, a few cases with bioinactive and immunoreactive GH have also been reported. We describe here a new bioassay system for GH determination using the BaF/GM cell line, which proliferates in a dose-dependent manner on hGH addition; cell proliferation was blocked by anti-hGH antibody. This bioassay had the lowest detection limit (∼0.02 ng/ml) reported thus far and the highest specificity for GH. The bioassay results were compared with those of an immunoradiometric assay across 163 patient samples in various endocrine states. A close correlation (the ratio of bioactivity/immunoreactivity was 1.04 ± 0.33, mean ± SD) was observed between bioactivity and immunoreactivity in these samples. The newly developed system is a specific, sensitive, easy, and fast bioassay system for GH determination; we consider it useful for evaluating GH bioactivity in various endocrine states.
authors.affiliation: Department of Pediatrics, Asahikawa Medical University, Asahikawa, Hokkaido, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Maimaiti
authors.forename: Y
authors.initials: Y
authors.lastname: Tanahashi
authors.forename: Z
authors.initials: Z
authors.lastname: Mohri
authors.forename: K
authors.initials: K
authors.lastname: Fujieda
mesh_terms: D000293:Adolescent; D001681:Biological Assay / Q000379:methods*; D016022:Case-Control Studies; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D006130:Growth Disorders / Q000097:blood*; D019382:Human Growth Hormone / Q000097:blood* / Q000494:pharmacology; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods*; D007223:Infant; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric
chemical_list: D019382:Human Growth Hormone