title: Proteomic profiling of tumor-initiating cells in HT-29 human colorectal cancer cells.
abstract: Recent reports have suggested that tumors are organized in heterogeneous populations. Within these populations, a small subpopulation of cells is more capable of initiating malignancy; these are called cancer stem cells. In this study, HT-29 cells were sorted according to the presence or absence of the cancer stem cell marker CD133. We confirmed that CD133+ cells possessed higher clonogenicity compared to CD133- cells. Furthermore, proteomic analysis identified 10 proteins, including actin-related protein 2/3 complex subunit 5-like and profilin 2. In conclusion, our data demonstrated that the expression of specific proteins associated with metastasis and invasion in CD133+ cells contributed to the stemness and tumorigenic properties of these cells.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, 5ga Anam-dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
authors.forename: Han-Na
authors.initials: HN
authors.lastname: Lee
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Lee
authors.forename: Raymundo
authors.initials: R
authors.lastname: Bernardo
authors.forename: Chan-Wha
authors.initials: CW
authors.lastname: Kim
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology; D002999:Clone Cells / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D006023:Glycoproteins / Q000378:metabolism*; D019073:HT29 Cells; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology*; D010455:Peptides / Q000378:metabolism*; D040081:Protein Array Analysis; D040901:Proteomics
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides