title: The interaction of banana MADS-box protein MuMADS1 and ubiquitin-activating enzyme E-MuUBA in post-harvest banana fruit.
abstract: KEY MESSAGE                 :                                     The interaction of MuMADS1 and MuUBA in banana was reported, which will help us to understand the mechanism of the MADS-box gene in regulating banana fruit development and ripening.                                                                  The ubiquitin-activating enzyme E1 gene fragment MuUBA was obtained from banana (Musa acuminata L.AAA) fruit by the yeast two-hybrid method using the banana MADS-box gene MuMADS1 as bait and 2-day post-harvest banana fruit cDNA library as prey. MuMADS1 interacted with MuUBA. The interaction of MuMADS1 and MuUBA in vivo was further proved by bimolecular fluorescence complementation assay. Real-time quantitative PCR evaluation of MuMADS1 and MuUBA expression patterns in banana showed that they are highly expressed in the ovule 4 stage, but present in low levels in the stem, which suggests a simultaneously differential expression action exists for both MuMADS1 and MuUBA in different tissues and developmental fruits. MuMADS1 and MuUBA expression was highly stimulated by exogenous ethylene and suppressed by 1-methylcyclopropene. These results indicated that MuMADS1 and MuUBA were co-regulated by ethylene and might play an important role in post-harvest banana fruit ripening.
authors.affiliation: Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, 4 Xueyuan Road, Haikou 571101, China.
authors.forename: Ju-Hua
authors.initials: JH
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Jia
authors.forename: Jian-Bin
authors.initials: JB
authors.lastname: Zhang
authors.forename: Jia-Shui
authors.initials: JS
authors.lastname: Wang
authors.forename: Zi-Xian
authors.initials: ZX
authors.lastname: Yang
authors.forename: Bi-Yu
authors.initials: BY
authors.lastname: Xu
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D003521:Cyclopropanes / Q000494:pharmacology; D005030:Ethylenes / Q000494:pharmacology; D005638:Fruit / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D028521:Musa / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D044764:Ubiquitin-Activating Enzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D003521:Cyclopropanes; D005030:Ethylenes; D026161:MADS Domain Proteins; D010940:Plant Proteins; C036216:ethylene; D044764:Ubiquitin-Activating Enzymes; C412563:1-methylcyclopropene