title: Crystal structure of mouse RhoA:GTPγS complex in a centered lattice.
abstract: RhoA, a member of the Rho sub-family of small GTPases, plays a significant signaling role in cell morphogenesis, migration, neuronal development, cell division and adhesion. So far, 4 structures of RhoA:GDP/GTP analogs and 14 structures of RhoA in complex with other proteins have been reported. All RhoA:GDP/GTP analog complexes have been crystallized in primitive lattices and RhoA is monomeric. This is the first time a RhoA:GTP analog complex has been crystallized as a dimer in a centered lattice. The present structure reveals structural differences in the switch-I (residues 28-42) and switch-II (residues 61-66) regions, which play important roles in interactions with downstream targets to transduce signals, when compared to the previously reported structures.
authors.affiliation: Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
authors.forename: Chacko
authors.initials: C
authors.lastname: Jobichen
authors.forename: Kuntal
authors.initials: K
authors.lastname: Pal
authors.forename: Kunchithapadam
authors.initials: K
authors.lastname: Swaminathan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D018360:Crystallography, X-Ray / Q000379:methods*; D004789:Enzyme Activation; D020558:GTP Phosphohydrolases / Q000737:chemistry; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000737:chemistry*; D006153:Guanosine Diphosphate / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000235:genetics; D025941:Protein Interaction Mapping; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D020741:rho GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D020742:rhoA GTP-Binding Protein
chemical_list: D046912:Multiprotein Complexes; D006153:Guanosine Diphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D020558:GTP Phosphohydrolases; C526380:RhoA protein, mouse; D020741:rho GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein