title: Measurement of cytosolic-free Ca²⁺ in plant tissue.
abstract: A range of techniques have been used to measure the concentration of cytosolic-free Ca(2+) ([Ca(2+)](cyt)) in plant cells. Fluorescent Ca(2+)-sensitive indicators have been used extensively to measure plant [Ca(2+)](cyt) and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca(2+)](cyt) in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca(2+)-sensitive indicator fura-2.
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster, UK. m.mcainsh@lancaster.ac.uk
authors.forename: Martin R
authors.initials: MR
authors.lastname: McAinsh
authors.forename: Carl K-Y
authors.initials: CK
authors.lastname: Ng
mesh_terms: D002118:Calcium / Q000378:metabolism*; D034822:Commelina / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D016257:Fura-2 / Q000378:metabolism; D010944:Plants / Q000378:metabolism*
chemical_list: D002118:Calcium; D016257:Fura-2