title: [Genetic relationship on several medicinal plants in Marsdenia from Yunnan in ISSR marker].
abstract: OBJECTIVE
  To analyze the genetic relationship of 9 Marsdenia species from Yunnan, especially the traditional Dai medicine "Dai Bai Jie" (M. auricularis).
  
  
  METHODS
  Applying the inter-simple sequence repeats (ISSR) markers technology.
  
  
  RESULTS
  Twenty-five primers were screened out of 60 ISSR random primers and produced 391 bands totally, every primer produce 8-21 bands and the mean number was 15.6. The range of the GS (genetic similarity) value was 0.6675-0.8210. In 9 Marsdenia species, M. auricularis is a relative of M. tenacissima. M. balansae and M. officinalis have the closest genetic relationship.
  
  
  CONCLUSIONS
  It is supported by ISSR that the M. auricularis which is sib species of M. tenacissima, and the folk medicine of Marsdenia are worthy deep investigation and study.
authors.affiliation: Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Jinghong 666100, China. zbguan@yahoo.com.cn
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Guan
authors.forename: Lixia
authors.initials: L
authors.lastname: Zhang
authors.forename: Meifang
authors.initials: M
authors.lastname: Song
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.forename: Zhonglian
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D002681:China; D005819:Genetic Markers / Q000235:genetics; D031101:Marsdenia / Q000145:classification* / Q000235:genetics*; D008516:Medicine, Chinese Traditional; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D005819:Genetic Markers