title: Self-recognition of high-mannose type glycans mediating adhesion of embryonal fibroblasts.
abstract: High-mannose type N-linked glycan with 6 mannosyl residues, termed "M6Gn2", displayed clear binding to the same M6Gn2, conjugated with ceramide mimetic (cer-m) and incorporated in liposome, or coated on polystyrene plates. However, the conjugate of M6Gn2-cer-m did not interact with complex-type N-linked glycan with various structures having multiple GlcNAc termini, conjugated with cer-m. The following observations indicate that hamster embryonic fibroblast NIL-2 K cells display homotypic autoadhesion, mediated through the self-recognition capability of high-mannose type glycans expressed on these cells: (i) NIL-2 K cells display clear binding to lectins capable of binding to high-mannose type glycans (e.g., ConA), but not to other lectins capable of binding to other carbohydrates (e.g. GS-II). (ii) NIL-2 K cells adhere strongly to plates coated with M6Gn2-cer-m, but not to plates coated with complex-type N-linked glycans having multiple GlcNAc termini, conjugated with cer-m; (iii) degree of NIL-2 K cell adhesion to plates coated with M6Gn2-cer-m showed a clear dose-dependence on the amount of M6Gn2-cer-m; and (iv) the degree of NIL-2 K adhesion to plates coated with M6Gn2-cer-m was inhibited in a dose-dependent manner by α1,4-L-mannonolactone, the specific inhibitor in high-mannose type glycans addition. These data indicate that adhesion of NIL-2 K is mediated by self-aggregation of high mannose type glycan. Further studies are to be addressed on auto-adhesion of other types of cells based on self interaction of high mannose type glycans.
authors.affiliation: Division of Biomembrane Research, Pacific Northwest Research Institute, and Department of Global Health, University of Washington, Seattle, WA 98122, USA.
authors.forename: Seon-Joo
authors.initials: SJ
authors.lastname: Yoon
authors.forename: Natalia
authors.initials: N
authors.lastname: Utkina
authors.forename: Martin
authors.initials: M
authors.lastname: Sadilek
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yagi
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
authors.forename: Sen-itiroh
authors.initials: SI
authors.lastname: Hakomori
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002518:Ceramides / Q000737:chemistry; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects*; D006001:Glycoconjugates / Q000737:chemistry; D007783:Lactones / Q000494:pharmacology*; D008358:Mannose / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D018716:Molecular Mimicry; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D002518:Ceramides; D006001:Glycoconjugates; D007783:Lactones; D011134:Polysaccharides; C053275:mannono-1,5-lactone; D008358:Mannose