title: MicroRNA expression profile analysis reveals diagnostic biomarker for human prostate cancer.
abstract: Prostate cancer is a highly prevalent disease in older men of the western world. MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression via posttranscriptional inhibition of protein synthesis. To identify the diagnostic potential of miRNAs in prostate cancer, we downloaded the miRNA expression profile of prostate cancer from the GEO database and analysed the differentially expressed miRNAs (DE-miRNAs) in prostate cancerous tissue compared to non-cancerous tissue. Then, the targets of these DE-miRNAs were extracted from the database and mapped to the STRING and KEGG databases for network construction and pathway enrichment analysis. We identified a total of 16 miRNAs that showed a significant differential expression in cancer samples. A total of 9 target genes corresponding to 3 DE-miRNAs were obtained. After network and pathway enrichment analysis, we finally demonstrated that miR-20 appears to play an important role in the regulation of prostate cancer onset. MiR-20 as single biomarker or in combination could be useful in the diagnosis of prostate cancer. We anticipate our study could provide the groundwork for further experiments.
authors.affiliation: Department of Urology, Yantai Yuhuangding Hospital, Yantai, China.
authors.forename: Dong-Fu
authors.initials: DF
authors.lastname: Liu
authors.forename: Ji-Tao
authors.initials: JT
authors.lastname: Wu
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Wang
authors.forename: Qing-Zuo
authors.initials: QZ
authors.lastname: Liu
authors.forename: Zhen-Li
authors.initials: ZL
authors.lastname: Gao
authors.forename: Yun-Xiang
authors.initials: YX
authors.lastname: Liu
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics*; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs