title: [Quantification of the curing effects of phenanthridine on yeast prion [PSI+]].
abstract: In order to quantify the curing effects of phenanthridine on yeast prion, we introduced semi-denaturing agarose gel electrophoresis and fluorescence recovery after photobleaching techniques to quantify the curing effects of phenanthridine on yeast prion at the protein and cellular levels with the [PSI+] yeast strain expressing GFP-Sup35p (NGMC). The results showed that these two approaches could precisely quantify the curing effects of phenanthridine on [PSI+] cells. After a treatment for 1 through 5 days with phenanthridine, the curing rates of [PSI+] cells were 0%, 0%, 51.7%, 87.5% and 94.4%, respectively. Meanwhile, we quantified the sizes of Sup35p polymers in phenanthridine induced pink phenotype cells. The aggregation status in 1-2 days phenanthridine treated cells were similar to those in [PSI+] cells, while the aggregation status in 3-5 days phenanthridine treated cells were similar to those in [psi(-)] cells.
authors.affiliation: School of Life Science, Liaoning University, Shenyang 110036, Liaoning, China.
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Zhong
authors.forename: Lijie
authors.initials: L
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Jianwei
authors.initials: J
authors.lastname: He
authors.forename: Youtao
authors.initials: Y
authors.lastname: Song
mesh_terms: D003198:Computer Simulation; D008954:Models, Biological; D010454:Peptide Termination Factors / Q000378:metabolism*; D010617:Phenanthridines / Q000494:pharmacology*; D011328:Prions / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D010454:Peptide Termination Factors; D010617:Phenanthridines; D011328:Prions; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins