title: Human pluripotent stem cell-derived mesenchymal stem cells prevent allergic airway inflammation in mice.
abstract: We previously found that mesenchymal stem cells (MSCs) derived from human-induced pluripotent stem cells (iPSCs) exerted immunomodulatory effects on Th2-mediated allergic rhinitis in vitro. However, their contribution to the asthma and allergic rhinitis in animal models remains unclear. In this study, we developed a mouse model of ovalbumin (OVA)-induced allergic inflammation in both the upper and lower airways and evaluated the effects of the systemic administration of human iPSC-MSCs and bone marrow-derived MSCs (BM-MSCs) on allergic inflammation. Our results showed that treatments with both the iPSC-MSCs and BM-MSCs before the challenge phase protected the animals from the majority of allergy-specific pathological changes. This protection included an inhibition of inflammatory cell infiltration and mucus production in the lung, a reduction in eosinophil infiltration in the nose, and a decrease in inflammatory cell infiltration in both the bronchoalveolar and nasal lavage fluids. In addition, treatment with iPSC-MSCs or BM-MSCs before the challenge phase resulted in reduced serum levels of Th2 immunoglobulins (e.g., IgE) and decreased levels of Th2 cytokines including interleukin (IL)-4, IL-5, or IL-13 in the bronchoalveolar and/or nasal lavage fluids. Similar therapeutic effects were observed when the animals were pretreated with human iPSC-MSCs before the sensitization phase. These data suggest that iPSC-MSCs may be used as an alternative strategy to adult MSCs in the treatment of asthma and allergic rhinitis.
authors.affiliation: Otorhinolaryngology Hospital, Sun Yat-sen University, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Yue-Qi
authors.initials: YQ
authors.lastname: Sun
authors.forename: Meng-Xia
authors.initials: MX
authors.lastname: Deng
authors.forename: Jia
authors.initials: J
authors.lastname: He
authors.forename: Qing-Xiang
authors.initials: QX
authors.lastname: Zeng
authors.forename: Weiping
authors.initials: W
authors.lastname: Wen
authors.forename: David S H
authors.initials: DS
authors.lastname: Wong
authors.forename: Hung-Fat
authors.initials: HF
authors.lastname: Tse
authors.forename: Geng
authors.initials: G
authors.lastname: Xu
authors.forename: Qizhou
authors.initials: Q
authors.lastname: Lian
authors.forename: Jianbo
authors.initials: J
authors.lastname: Shi
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Fu
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology / Q000601:surgery / Q000628:therapy*; D016535:Bronchial Hyperreactivity / Q000276:immunology / Q000601:surgery / Q000628:therapy*; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology; D005260:Female; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D016219:Immunotherapy, Adoptive; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009296:Nasal Cavity / Q000276:immunology; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000276:immunology* / Q000637:transplantation*; D018418:Th2 Cells / Q000276:immunology
chemical_list: D016207:Cytokines; D007073:Immunoglobulin E