title: Genotyping SSR length variants by isothermal DNA amplification.
abstract: Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target.
authors.affiliation: John Bingham Laboratory, National Institute of Agricultural Botany, Cambridge CB3 0LE, UK. david.lee@niab.com
authors.forename: David
authors.initials: D
authors.lastname: Lee
authors.forename: Manuela
authors.initials: M
authors.lastname: Ialicicco
authors.forename: Harika
authors.initials: H
authors.lastname: Akkinepalli
authors.forename: Giacomo
authors.initials: G
authors.lastname: Morreale
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Hei
authors.initials: H
authors.lastname: Leung
authors.forename: Gabriella S
authors.initials: GS
authors.lastname: Scippa
authors.forename: Andy
authors.initials: A
authors.lastname: Greenland
authors.forename: Ian
authors.initials: I
authors.lastname: Mackay
mesh_terms: D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D060005:Genotyping Techniques; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012275:Oryza / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant