title: Determination of nine environmental phenols in urine by ultra-high-performance liquid chromatography-tandem mass spectrometry.
abstract: A method was developed to determine nine environmental phenols, including bisphenol A, 2,3,4-trichlorophenol, 2,4,5-trichlorophenol, pentachlorophenol, triclosan (2,4,4'-trichloro-2'-hydroxyphenylether), 4-tert-octylphenol, 4-n-octylphenol, 4-n-nolyphenol and benzophenone-3 (2-hydroxy-4-methoxybenzophenone) in human urine using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS). The analytes were extracted and preconcentrated with solid-phase extraction, and then quantified with UPLC-electrospray ionization (negative ion mode)-MS-MS using multiple reaction monitoring mode. Limits of detection of the nine phenols ranged from 0.02 to 0.90 ng/mL. This method was further validated by the determination of phenols in 325 human urine samples that generated data regarding the exposure of various phenols to Chinese adults without occupational exposure to phenols.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Minjian
authors.initials: M
authors.lastname: Chen
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhu
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Rencheng
authors.initials: R
authors.lastname: Zhao
authors.forename: Shanlei
authors.initials: S
authors.lastname: Qiao
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Chen
authors.forename: Rong
authors.initials: R
authors.lastname: Tang
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Ling
authors.initials: L
authors.lastname: Song
authors.forename: Shoulin
authors.initials: S
authors.lastname: Wang
authors.forename: Yankai
authors.initials: Y
authors.lastname: Xia
authors.forename: Xinru
authors.initials: X
authors.lastname: Wang
mesh_terms: D001559:Benzhydryl Compounds / Q000652:urine; D001577:Benzophenones / Q000652:urine; D002733:Chlorophenols / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004781:Environmental Exposure / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D010416:Pentachlorophenol / Q000652:urine; D010636:Phenols / Q000652:urine*; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry / Q000379:methods; D014260:Triclosan / Q000652:urine; D016482:Urinalysis / Q000379:methods; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D001577:Benzophenones; D002733:Chlorophenols; D010416:Pentachlorophenol; D010636:Phenols; D014260:Triclosan; C009534:2,4,5-trichlorophenol; C080417:4-octylphenol; C502485:2,3,4-trichlorophenol; C005290:oxybenzone; C105260:4-tert-octylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds