title: Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex.
abstract: Here we describe the function of a previously uncharacterized protein, named family with sequence similarity 60 member A (FAM60A) that maps to chromosome 12p11 in humans. We use quantitative proteomics to determine that the main biochemical partners of FAM60A are subunits of the Sin3 deacetylase complex and show that FAM60A resides in active HDAC complexes. In addition, we conduct gene expression pathway analysis and find that FAM60A regulates expression of genes that encode components of the TGF-beta signaling pathway. Moreover, our studies reveal that loss of FAM60A or another component of the Sin3 complex, SDS3, leads to a change in cell morphology and an increase in cell migration. These studies reveal the function of a previously uncharacterized protein and implicate the Sin3 complex in suppressing cell migration.
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
authors.forename: Karen T
authors.initials: KT
authors.lastname: Smith
authors.forename: Mihaela E
authors.initials: ME
authors.lastname: Sardiu
authors.forename: Skylar A
authors.initials: SA
authors.lastname: Martin-Brown
authors.forename: Chris
authors.initials: C
authors.lastname: Seidel
authors.forename: Arcady
authors.initials: A
authors.lastname: Mushegian
authors.forename: Rhonda
authors.initials: R
authors.lastname: Egidy
authors.forename: Laurence
authors.initials: L
authors.lastname: Florens
authors.forename: Michael P
authors.initials: MP
authors.lastname: Washburn
authors.forename: Jerry L
authors.initials: JL
authors.lastname: Workman
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D056945:Hep G2 Cells; D056572:Histone Deacetylase Inhibitors / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D017434:Protein Structure, Tertiary; D034622:RNA Interference; D034741:RNA, Small Interfering; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics; D012097:Repressor Proteins / Q000378:metabolism*; D020539:Sequence Analysis, Protein; D056264:Sin3 Histone Deacetylase and Corepressor Complex / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D056572:Histone Deacetylase Inhibitors; D018398:Homeodomain Proteins; C444654:ING2 protein, human; D034741:RNA, Small Interfering; D018160:Receptors, Cytoplasmic and Nuclear; D012097:Repressor Proteins; C576636:SINHCAF protein, human; C576731:SUDS3 protein, human; D016212:Transforming Growth Factor beta; D025521:Tumor Suppressor Proteins; D056264:Sin3 Histone Deacetylase and Corepressor Complex