title: Layer-by-layer self-assembled graphene oxide/silica microsphere composites as stationary phase for high performance liquid chromatography.
abstract: Graphene oxide (GO) has been layer-by-layer assembled onto silica microspheres to form a GO/SiO(2) composite stationary phase. All the characterizations of GO/SiO(2) by elemental analysis, Raman spectroscopy and Fourier transformed infrared spectrometry confirmed that with the increase of the assembled layer, GO gradually increases on the silica surface. The chromatographic properties of bare SiO(2) and GO/SiO(2) with different GO assembled layers show that the amount of GO plays an important role in the separation of analytes. Only the appropriate amount of GO on SiO(2) can perform a good chromatographic separation. The comparison between chromatographic performances of bare SiO(2) column, GO/SiO(2)-2 column and C18 commercial column clearly show that GO/SiO(2)-2 and C18 columns obtained a better separation; GO/SiO(2)-2 exhibits a large π-electron system and C18 exhibits hydrophobicity. The eluting order, peak width and resolution of analyte on GO/SiO(2)-2 column was highly dependent on the size of its π-electron system, while on the C18 column the decisive factor is its hydrophobic property.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Liang
authors.forename: Shujuan
authors.initials: S
authors.lastname: Liu
authors.forename: Xinwang
authors.initials: X
authors.lastname: Song
authors.forename: Yangwen
authors.initials: Y
authors.lastname: Zhu
authors.forename: Shengxiang
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000588:Amines / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D006108:Graphite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D010087:Oxides / Q000737:chemistry*; D019800:Phenol / Q000302:isolation & purification; D011084:Polycyclic Aromatic Hydrocarbons / Q000302:isolation & purification; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000588:Amines; D010087:Oxides; D011084:Polycyclic Aromatic Hydrocarbons; D019800:Phenol; D012822:Silicon Dioxide; D006108:Graphite