title: Genome-wide chromatin immunoprecipitation-sequencing in Plasmodium.
abstract: Chromatin immunoprecipitation (ChIP) studies have been used extensively in recent years to study the functional role of histone marks, variant histones, and other chromatin factors in gene expression in the human malaria parasite, Plasmodium falciparum. In this chapter, we present a ChIP-sequencing protocol optimized for blood-stage forms of this parasite. The processing of the immunoprecipitated DNA prior to high-throughput sequencing is performed in a way to minimize amplification biases due to the high genomic AT-content of the parasite.
authors.affiliation: Unité de Biologie des Interactions Hôte-Parasite, Institut Pasteur, Paris, France.
authors.forename: Jose-Juan
authors.initials: JJ
authors.lastname: Lopez-Rubio
authors.forename: T Nicolai
authors.initials: TN
authors.lastname: Siegel
authors.forename: Artur
authors.initials: A
authors.lastname: Scherf
mesh_terms: D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D047369:Chromatin Immunoprecipitation / Q000379:methods*; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D018503:Genome, Protozoan; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D010961:Plasmodium / Q000235:genetics*
chemical_list: D002843:Chromatin; D016054:DNA, Protozoan