title: In vitro immunomodulatory properties of osteogenic and adipogenic differentiated mesenchymal stem cells isolated from three inbred mouse strains.
abstract: Mesenchymal stem cells (MSCs) are used for cell-based therapies because of their immunomodulatory properties. The immunomodulatory properties of adipogenic (AD) and osteogenic (OS) differentiated adipose tissue-derived MSCs (AD-MSCs) isolated from BALB/c, C57BL/6, and DBA mice were compared. Splenocytes proliferation was suppressed in the presence of AD-MSCs conditioned media in all mice. After OS differentiation, BALB/c AD-MSCs produced higher levels of TGF-β and IL-17 and lower levels of NO than AD-MSCs isolated from C57BL/6 and DBA mice. In addition, OS differentiated AD-MSCs isolated from DBA mice produced lower levels of IL-10 than AD-MSCs isolated from C57BL/6 and DBA mice. After in vitro AD and OD differentiation, AD-MSCs isolated from each mouse produced higher levels of NO and IDO than undifferentiated cells. Additionally, AD-MSCs isolated from C57BL/6 and DBA mice produced higher levels of NO than AD-MSCs isolated from BALB/c mice. Adipose tissue-derived MSCs thus retain their immunomodulatory properties after in vitro OS and AD differentiation in a strain-dependent manner.
authors.affiliation: Department of Immunology, Tarbiat Modares University, Tehran, Iran.
authors.forename: Seyed Mahmoud
authors.initials: SM
authors.lastname: Hashemi
authors.forename: Zuhair Mohammad
authors.initials: ZM
authors.lastname: Hassan
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Pourfathollah
authors.forename: Sara
authors.initials: S
authors.lastname: Soudi
authors.forename: Abbas
authors.initials: A
authors.lastname: Shafiee
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis / Q000502:physiology; D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D006651:Histocytochemistry; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000032:analysis / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D010011:Osteocytes / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology; D013154:Spleen / Q000166:cytology
chemical_list: D015703:Antigens, CD; D017077:Culture Media, Conditioned; D016207:Cytokines; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D009569:Nitric Oxide