title: RNA-based diagnosis in a multicellular specimen by whole mount in situ hybridization using an RNA-specific probe.
abstract: Recent RNA research has revealed the close involvement of various RNAs in cellular functions. RNAs are becoming the inevitable target molecules for research into details of gene expression. RNA and its related complexes are also promising targets for disease diagnosis. Multi cellular specimens such as organ tissues, histopathological specimens, and embryos are among the possible targets of RNA-based diagnostic techniques. In this report, we focused on a method that would provide such spatial and temporal information. We demonstrated that an RNA-specific probe (OMUpy2) was not only applicable to the detection of a specific mRNA in Drosophila embryos in a temporal and spatial manner but was also relatively quick and easy to use. The probe, OMUpy2, could be applied to other multi cellular systems for RNA-based diagnosis and research. The promising results of this manuscript show the great potential of RNA-based detection for both biological research and diagnostic medicine.
authors.affiliation: Department of Biomolecular Engineering, Kyoto Institutes of Technology, Matsugasaki, Kyoto 606-8585, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Ueda
authors.forename: Akio
authors.initials: A
authors.lastname: Kobori
authors.forename: Asako
authors.initials: A
authors.lastname: Yamayoshi
authors.forename: Hideki
authors.initials: H
authors.lastname: Yoshida
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Akira
authors.initials: A
authors.lastname: Murakami
mesh_terms: D000818:Animals; D004330:Drosophila / Q000254:growth & development / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D017403:In Situ Hybridization; D009843:Oligoribonucleotides / Q000737:chemistry / Q000378:metabolism*; D011721:Pyrenes / Q000737:chemistry / Q000378:metabolism*; D015347:RNA Probes / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: C578256:OMUpy2; D009843:Oligoribonucleotides; D011721:Pyrenes; D015347:RNA Probes; D012333:RNA, Messenger