title: Growth properties of SF188/V+ human glioma in rats in vivo observed by magnetic resonance imaging.
abstract: SF188/V+ is a highly vascular human glioma model that is based on transfection of vascular endothelial growth factor (VEGF) cDNA into SF188/V- cells. This study aims to assess its growth and vascularity properties in vivo in a rat model. Thirty-two adult rats were inoculated with SF188/V+ tumor cells, and, for comparison, five were inoculated with SF188/V- tumor cells. Several conventional magnetic resonance imaging (MRI) sequences were acquired, and several quantitative structural (T(2) and T(1)), functional [isotropic apparent diffusion coefficient (ADC) and blood flow], and molecular [protein and peptide-based amide proton transfer (APT)] MRI parameters were mapped on a 4.7 T animal scanner. In rats inoculated with SF188/V+ tumor cells, conventional T(2)-weighted images showed a highly heterogeneous tumor mass, and post-contrast T(1)-weighted images showed a heterogeneous, strong enhancement of the mass. There were moderate increases in T(2), T(1), and ADC, and large increases in blood flow and APT in the tumor, compared to contralateral brain tissue. Microscopic examination revealed prominent vascularity and hemorrhage in the VEGF-secreting xenografts as compared to controls, and immunohistochemical staining confirmed increased expression of VEGF in tumor xenografts. Our results indicate that the SF188/V+ glioma model exhibits some MRI and histopathology features that closely resemble human glioblastoma.
authors.affiliation: Department of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
authors.forename: Rachel
authors.initials: R
authors.lastname: Grossman
authors.forename: Betty
authors.initials: B
authors.lastname: Tyler
authors.forename: Henry
authors.initials: H
authors.lastname: Brem
authors.forename: Charles G
authors.initials: CG
authors.lastname: Eberhart
authors.forename: Silun
authors.initials: S
authors.lastname: Wang
authors.forename: De-Xue
authors.initials: DX
authors.lastname: Fu
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Wen
authors.forename: Jinyuan
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics / Q000473:pathology*; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005910:Glioma / Q000000981:diagnostic imaging / Q000235:genetics / Q000473:pathology*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D009389:Neovascularization, Pathologic; D011859:Radiography; D051381:Rats; D011923:Rats, Nude; D014407:Tumor Cells, Cultured / Q000637:transplantation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat