title: Association study identifying polymorphisms in CD47 and other extracellular matrix pathway genes as putative prognostic markers for colorectal cancer.
abstract: OBJECTIVE
  We identified recently the extracellular matrix (ECM) receptor interaction pathway as a consistently overrepresented category among gene expression profiling studies on colorectal cancer (CRC) prognosis.
  
  
  METHODS
  Putative regulatory single nucleotide polymorphisms (SNPs) in genes from the ECM pathway were genotyped in 613 CRC patients from Northern Germany (PopGen cohort) and tested for association with disease progression and survival.
  
  
  RESULTS
  The eSNP (SNP associated with expression) rs12695175 in CD47 associated with CRC specific survival (HR = 2.18, 95 % CI 1.10-4.33, CC versus AA) and with overall survival (HR = 1.99, 95 % CI 1.04-3.81, CC versus AA). This association remained significant after adjustment for age at diagnosis, tumour stage (T) and lymph node status (N). Three polymorphisms in CD47 were associated with distant metastasis in a dominant model: rs9879947 and rs3206652 in the 3'-UTR (OR = 1.64, 95 % CI 1.01-2.64 and OR = 1.88, 95 % CI 1.27-2.80, respectively) and the eSNP rs3804639 (OR = 1.73, 95 % CI 1.17-2.57).
  
  
  CONCLUSIONS
  The novel associations of eSNPs in CD47 with worse survival and distant metastasis should be confirmed by additional studies, since increased expression of this gene has recently been shown to be an indicator of poor prognosis in cancer patients.
authors.affiliation: Division of Molecular Genetic Epidemiology, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 580, 69120, Heidelberg, Germany.
authors.forename: Jesús
authors.initials: J
authors.lastname: Lascorz
authors.forename: Melanie
authors.initials: M
authors.lastname: Bevier
authors.forename: Witigo
authors.initials: W
authors.lastname: V Schönfels
authors.forename: Holger
authors.initials: H
authors.lastname: Kalthoff
authors.forename: Heiko
authors.initials: H
authors.lastname: Aselmann
authors.forename: Jan
authors.initials: J
authors.lastname: Beckmann
authors.forename: Jan
authors.initials: J
authors.lastname: Egberts
authors.forename: Stephan
authors.initials: S
authors.lastname: Buch
authors.forename: Thomas
authors.initials: T
authors.lastname: Becker
authors.forename: Stefan
authors.initials: S
authors.lastname: Schreiber
authors.forename: Jochen
authors.initials: J
authors.lastname: Hampe
authors.forename: Kari
authors.initials: K
authors.lastname: Hemminki
authors.forename: Clemens
authors.initials: C
authors.lastname: Schafmayer
authors.forename: Asta
authors.initials: A
authors.lastname: Försti
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D051928:CD47 Antigen / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D018450:Disease Progression; D005109:Extracellular Matrix / Q000235:genetics*; D005260:Female; D052138:Genes, Neoplasm / Q000235:genetics; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D015398:Signal Transduction / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; D051928:CD47 Antigen; C497894:CD47 protein, human