title: DNA bar-coding for phytoplasma identification.
abstract: Phytoplasma identification has proved difficult due to their inability to be maintained in vitro. DNA barcoding is an identification method based on comparison of a short DNA sequence with known sequences from a database. A DNA barcoding tool has been developed for phytoplasma identification. While other sequence-based methods may be well adapted to identification of particular strains of phytoplasmas, often they cannot be used for the simultaneous identification of phytoplasmas from different groups. The phytoplasma DNA barcoding protocol in this chapter, based on the tuf and 16SrRNA genes, can be used to identify the following phytoplasma groups: 16SrI, 16SrII, 16SrIII, 16SrIV, 16SrV, 16SrVI, 16SrVII, 16SrIX, 16SrX, 16SrXI, 16SrXII, 16SrXV, 16SrXX, 16SrXXI.
authors.affiliation: Department of Agroecology, Aarhus University, Slagelse, Denmark.
authors.forename: Olga
authors.initials: O
authors.lastname: Makarova
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Contaldo
authors.forename: Samanta
authors.initials: S
authors.lastname: Paltrinieri
authors.forename: Assunta
authors.initials: A
authors.lastname: Bertaccini
authors.forename: Henriette
authors.initials: H
authors.lastname: Nyskjold
authors.forename: Mogens
authors.initials: M
authors.lastname: Nicolaisen
mesh_terms: D001483:Base Sequence; D019295:Computational Biology / Q000379:methods; D004247:DNA / Q000302:isolation & purification; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry*; D030561:Databases, Nucleic Acid; D005798:Genes, Bacterial; D020407:Internet; D008969:Molecular Sequence Data; D045582:Phytoplasma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D004247:DNA