title: Arginine exposure alters ectonucleotidase activities and morphology of zebrafish larvae (Danio rerio).
abstract: Hyperargininemia is an inborn error of metabolism (IEM) characterized by tissue accumulation of arginine (Arg). Mental retardation and other neurological features are common symptoms in hyperargininemic patients. Considering purinergic signaling has a crucial role from the early stages of development and underlying mechanisms of this disease are poorly established, we investigated the effect of Arg administration on locomotor activity, morphological alterations, and extracellular nucleotide hydrolysis in larvae and adult zebrafish. We showed that 0.1 mM Arg was unable to promote changes in locomotor activity. In addition, 7-day-post-fertilization (dpf) larvae treated with Arg demonstrated a decreased body size. Arg exposure (0.1 mM) promoted an increase in ATP, ADP, and AMP hydrolysis when compared to control group. These findings demonstrated that Arg might affect morphological parameters and ectonucleotidase activities in zebrafish larvae, suggesting that purinergic system is a target for neurotoxic effects induced by Arg.
authors.affiliation: Laboratório de Neuroquímica e Psicofarmacologia, Departamento de Biologia Celular e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Katiucia Marques
authors.initials: KM
authors.lastname: Capiotti
authors.forename: Lidiane
authors.initials: L
authors.lastname: Fazenda
authors.forename: Luiza Reali
authors.initials: LR
authors.lastname: Nazario
authors.forename: Fabiano Peres
authors.initials: FP
authors.lastname: Menezes
authors.forename: Luiza Wilges
authors.initials: LW
authors.lastname: Kist
authors.forename: Maurício Reis
authors.initials: MR
authors.lastname: Bogo
authors.forename: Rosane Souza
authors.initials: RS
authors.lastname: Da Silva
authors.forename: Angela Terezinha
authors.initials: AT
authors.lastname: Wyse
authors.forename: Carla Denise
authors.initials: CD
authors.lastname: Bonan
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000367:Age Factors; D000704:Analysis of Variance; D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D007814:Larva / Q000187:drug effects*; D009043:Motor Activity / Q000187:drug effects; D009708:Nucleotidases / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D001120:Arginine; D009708:Nucleotidases; C030287:nucleotidase