title: Bioactive secondary metabolites from Salix tetrasperma Roxb.
abstract: Column chromatography of the light petroleum fraction from the methanolic extract of the stem bark of Salix tetrasperma Roxb. (Salicaceae) resulted in the isolation of beta-sitosterol acetate, friedelin, 3beta-friedelinol, beta-amyrin, beta-sitosterol, beta-sitosterol-O-glucoside in addition to palmitic acid. From the dichloromethane fraction of the leaves, catechol and tremulacin were isolated. Salicin and its derivatives tremuloidin and 2'-O-p-(E)-coumaroyl salicin were isolated from the ethyl acetate fraction of the leaves. The isolated compounds were identified by MS, and 1D NMR (1H and 13C) and 2D NMR (H-H COSY, HSQC, and HMBC) spectral analyses. The total methanolic extract exhibited significant anti-inflammatory activity (rat hind paw oedema). The extract with a content of 120 mg/kg body weight produced 52% inhibition equivalent to the standard diclofenac sodium (54% inhibition). The antioxidant (DPPH free radical scavenging) and analgesic activities, respectively, were also evaluated.
authors.affiliation: Pharmacognosy Department, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt. assemels2002@yahoo.co.uk
authors.forename: Assem
authors.initials: A
authors.lastname: El-Shazly
authors.forename: Afaf
authors.initials: A
authors.lastname: El-Sayed
authors.forename: Eman
authors.initials: E
authors.lastname: Fikrey
mesh_terms: D000818:Animals; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D032108:Salix / Q000378:metabolism*