title: Automated DNA extraction for large numbers of plant samples.
abstract: The method described here is a rapid, total DNA extraction procedure applicable to a large number of plant samples requiring pathogen detection. The procedure combines a simple and quick homogenization step of crude extracts with DNA extraction based upon the binding of DNA to magnetic beads. DNA is purified in an automated process in which the magnetic beads are transferred through a series of washing buffers. The eluted DNA is suitable for efficient amplification in PCR reactions.
authors.affiliation: National Institute of Biology, Ljubljana, Slovenia. natasa.mehle@nib.si
authors.forename: Nataša
authors.initials: N
authors.lastname: Mehle
authors.forename: Petra
authors.initials: P
authors.lastname: Nikolić
authors.forename: Matevž
authors.initials: M
authors.lastname: Rupar
authors.forename: Jana
authors.initials: J
authors.lastname: Boben
authors.forename: Maja
authors.initials: M
authors.lastname: Ravnikar
authors.forename: Marina
authors.initials: M
authors.lastname: Dermastia
mesh_terms: D057205:Automation, Laboratory; D004269:DNA, Bacterial / Q000302:isolation & purification*; D018744:DNA, Plant / Q000302:isolation & purification*; D045582:Phytoplasma / Q000235:genetics; D010944:Plants / Q000235:genetics / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant