title: MSP58 knockdown inhibits the proliferation of esophageal squamous cell carcinoma in vitro and in vivo.
abstract: Esophageal carcinoma (EC) is one of the most aggressive cancers with a poor prognosis. Understanding the molecular mechanisms underlying esophageal cancer progression is a high priority for improved EC diagnosis and prognosis. Recently, MSP58 was shown to behave as an oncogene in colorectal carcinomas and gliomas. However, little is known about its function in esophageal carcinomas. We therefore examined the effects of MSP58 knockdown on the growth of esophageal squamous cell carcinoma (ESCC) cells in vitro and in vivo in order to gain a better understanding of its potential as a tumor therapeutic target. We employed lentiviral-mediated small hairpin RNA (shRNA) to knock down the expression of MSP58 in the ESCC cell lines Eca-109 and EC9706 and demonstrated inhibition of ESCC cell proliferation and colony formation in vitro. Furthermore, flow cytometry and western blot analyses revealed that MSP58 depletion induced cell cycle arrest by regulating the expression of P21, CDK4 and cyclin D1. Notably, the downregulation of MSP58 significantly inhibited the growth of ESCC xenografts in nude mice. Our results suggest that MSP58 may play an important role in ESCC progression.
authors.affiliation: The State Key Laboratory of Cancer Biology, Department of Gastrointestinal Surgery, Xijing Hospital, the Fourth Military Medical University, Xi'an, China.
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Xu
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Zheng
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Zhang
authors.forename: Hua-Dong
authors.initials: HD
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Wu
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Wei-Zhong
authors.initials: WZ
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D059447:Cell Cycle Checkpoints / Q000235:genetics / Q000502:physiology; D048708:Cell Growth Processes / Q000235:genetics / Q000502:physiology; D045744:Cell Line, Tumor; D019938:Cyclin D1 / Q000235:genetics; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D015536:Down-Regulation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009687:Nuclear Proteins / Q000172:deficiency* / Q000235:genetics*; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000235:genetics*
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; C113292:MCRS1 protein, human; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D019938:Cyclin D1; D051358:Cyclin-Dependent Kinase 4