title: Reduced dermis thickness and AGE accumulation in diabetic abdominal skin.
abstract: Dermatological problems in diabetes might play an important role in the spontaneous ulcers and impaired wound healing that are seen in diabetic patients. Investigation of the cause of diabetic skin disorders is critical for identifying effective treatment. The abdominal full-thickness skin tissues of 33 patients (14 nondiabetic and 19 diabetic) were analyzed. The cell viability and malondialdehyde (MDA) production of fibroblasts were measured after advanced glycosylation end product (AGE)-bovine serum albumin (BSA) exposure. Cutaneous histological observation showed reduced thickness of the diabetic abdominal dermis with morphological characteristics of obscured multilayer epithelium and shortened, thinned, and disorganized collagen fibrils with focal chronic inflammatory cell infiltration when compared with controls of the same age. Accumulation of AGEs in diabetic skin was prominent. Less hydroxyproline, higher myeloperoxidase activity, and increased MDA content were detected in diabetic skin. In vitro, the time- and dose-dependent inhibitory effects of AGE-BSA on fibroblast viability as well as the fact that AGE-BSA could promote MDA production of fibroblasts were shown. It is shown that the accumulation of AGEs in diabetic skin tissue induces an oxidative damage of fibroblasts and acts as an important contributor to the thinner diabetic abdominal dermis. The authors believe that diabetic cutaneous properties at baseline may increase the susceptibility to injury, and diabetic wounds possess atypical origin in the repair process.
authors.affiliation: Shanghai Jiao Tong University School of Medicine, Shanghai, Peoples' Republic of China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Niu
authors.forename: Xiaozan
authors.initials: X
authors.lastname: Cao
authors.forename: Fei
authors.initials: F
authors.lastname: Song
authors.forename: Ting
authors.initials: T
authors.lastname: Xie
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Ji
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Miao
authors.forename: Jiaoyun
authors.initials: J
authors.lastname: Dong
authors.forename: Ming
authors.initials: M
authors.lastname: Tian
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Shuliang
authors.initials: S
authors.lastname: Lu
mesh_terms: D000005:Abdomen / Q000033:anatomy & histology / Q000473:pathology; D016022:Case-Control Studies; D048909:Diabetes Complications / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005347:Fibroblasts; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D012710:Serum Albumin, Bovine / Q000032:analysis; D012867:Skin / Q000033:anatomy & histology / Q000378:metabolism / Q000473:pathology; D012871:Skin Diseases / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors; D014945:Wound Healing
chemical_list: D017127:Glycation End Products, Advanced; D012710:Serum Albumin, Bovine; D008315:Malondialdehyde