title: Cytokine factors present in dengue patient sera induces alterations of junctional proteins in human endothelial cells.
abstract: Plasma leakage in severe dengue has been postulated to be associated with skewed cytokine immune responses. In this study, the association of cytokines with vascular permeability in dengue patients was investigated. Human serum samples collected from 48 persons (13 with dengue fever, 29 with dengue hemorrhagic fever, and 6 healthy) were subjected to cytokines analysis by using Luminex Multiplex Technology. Selected serum samples from patients with dengue hemorrhagic fever sera and recombinant human cytokines were then tested for roles on inducing vascular permeability by treatment of human umbilical vein endothelial cells. Confocal immunofluorescence staining indicated morphologic alteration of human umbilical vein endothelial cells treated with serum samples from patients with dengue hemorrhagic fever compared with serum samples from healthy persons. The findings suggest that cytokines produced during dengue hemorrhagic infections could induce alterations in the vascular endothelium, which may play a fundamental role in the pathophysiology of dengue.
authors.affiliation: Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia. ag_praba@yahoo.com
authors.forename: Ramapraba
authors.initials: R
authors.lastname: Appanna
authors.forename: Seok Mui
authors.initials: SM
authors.lastname: Wang
authors.forename: Sasheela A
authors.initials: SA
authors.lastname: Ponnampalavanar
authors.forename: Lucy Chai See
authors.initials: LC
authors.lastname: Lum
authors.forename: Shamala Devi
authors.initials: SD
authors.lastname: Sekaran
mesh_terms: D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood*; D003715:Dengue / Q000097:blood*; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans
chemical_list: D016207:Cytokines