title: TiO2 nanorods via one-step electrospinning technique: a novel nanomatrix for mouse myoblasts adhesion and propagation.
abstract: This study was aimed at the synthesis and characterization of novel Titania nanorods by sol-gel electrospinning technique. The physicochemical properties of the synthesized nanorods were determined by field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and X-ray diffraction (XRD) pattern. To examine the in vitro cytotoxicity, mouse myoblast C2C12 cells were treated with different concentrations of as prepared TiO(2) nanorods and the viability of cells was analyzed by Cell Counting Kit-8 assay at regular time intervals. The morphological features of the cells attached with nanorods were examined by Bio-SEM. Cytotoxicity experiments indicated that the mouse myoblast cells could attach to the TiO(2) nanorods after being cultured. We observed that TiO(2) nanorods could support cell adhesion and growth and guide spreading behavior of myoblasts. We conclude that the electrospun TiO(2) nanorods scaffolds with unique morphology had excellent biocompatibility. Thus, the current work demonstrates that the as-synthesized TiO(2) nanorods represent a promising biomaterial to be exploited for various tissue engineering applications.
authors.affiliation: Department of Animal Science, Institute of Rare Earth for Biological Application, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors.forename: Touseef
authors.initials: T
authors.lastname: Amna
authors.forename: M Shamshi
authors.initials: MS
authors.lastname: Hassan
authors.forename: Woon-Seob
authors.initials: WS
authors.lastname: Shin
authors.forename: Hoa
authors.initials: H
authors.lastname: Van Ba
authors.forename: Hak-Kyo
authors.initials: HK
authors.lastname: Lee
authors.forename: Myung-Seob
authors.initials: MS
authors.lastname: Khil
authors.forename: I H
authors.initials: IH
authors.lastname: Hwang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D007202:Indicators and Reagents; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D032446:Myoblasts / Q000187:drug effects*; D043942:Nanotubes; D054457:Tissue Scaffolds; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D007202:Indicators and Reagents; C009495:titanium dioxide; D014025:Titanium