title: Intracytoplasmic morphology-selected sperm injection.
abstract: Approximately 40% of sterility in couples can be attributed to male subfertility and intracytoplasmic sperm injection (ICSI) has become a powerful tool in assisted reproduction to overcome male infertility. Intracytoplasmic morphologically selected sperm injection (IMSI) is an advanced and sophisticated method of ICSI, where prior to sperm injection the morphology of the sperm is evaluated under high magnification. In addition, the IMSI procedure involves a few minor modifications in sperm preparation which are not carried out during the conventional ICSI procedure, such as the use of MSOME criteria, the requirement for a glass-bottomed dish for selection, prolonged sperm manipulation following separation from the seminal fluid, and sperm storage prior to microinjection. These variations are discussed in this chapter.
authors.affiliation: Andrology and IVF Laboratories, Division of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, UT, USA.
authors.forename: Luke
authors.initials: L
authors.lastname: Simon
authors.forename: Aaron
authors.initials: A
authors.lastname: Wilcox
authors.forename: Douglas T
authors.initials: DT
authors.lastname: Carrell
mesh_terms: D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D006801:Humans; D007248:Infertility, Male / Q000628:therapy; D008297:Male; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods* / Q000592:standards; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000166:cytology* / Q000473:pathology