title: Targeting essential genes in Salmonella enterica serovar typhimurium with antisense peptide nucleic acid.
abstract: We investigated the capability of antisense peptide nucleic acids (PNAs) conjugated to the (KFF)(3)K cell-penetrating peptide to target possible essential genes (ligA, rpoA, rpoD, engA, tsf, and kdtA) in Salmonella enterica serovar Typhimurium and inhibit bacterial growth in vitro and in cell culture. All targeted PNA-based gene inhibition has shown great potency in gene expression inhibition in a sequence-specific and dose-dependent manner at micromolar concentrations. Among tested PNAs, the anti-rpoA and -rpoD PNAs showed the greatest potency.
authors.affiliation: Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA.
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Soofi
authors.forename: Mohamed N
authors.initials: MN
authors.lastname: Seleem
mesh_terms: D016373:DNA, Antisense / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005798:Genes, Bacterial / Q000187:drug effects*; D009842:Oligopeptides / Q000235:genetics*; D020135:Peptide Nucleic Acids / Q000494:pharmacology*; D019779:Salmonella enterica / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development
chemical_list: C578530:(lysyl-phenylalanyl-phenylalanyl)3-lysine; D016373:DNA, Antisense; D009842:Oligopeptides; D020135:Peptide Nucleic Acids