title: Rapid detection of ovarian cancer from immunized serum using a quartz crystal microbalance immunosensor.
abstract: BACKGROUND
  The objective of this study was to measure the antibody content of NuTu-19 ovarian cancer cells in serum samples using a quartz crystal microbalance (QCM) immunosensor.
  
  
  METHODS
  NuTu-19 cells were first cultured onto the electrode surfaces of crystals in Dulbecco's modified Eagle medium, and then specified amounts of immunized serum samples of immunized rabbit were also added. The change in mass caused by specific adsorbtion of antibodies of NuTu-19 to the surfaces of the crystals was detected.
  
  
  RESULTS
  The change in resonance frequency of crystals caused by immobilization of NuTu-19 cells was from 83 to 429 Hz. The antibody content of NuTu-19 detected was 341 ng/ul. The frequency shifts were linearly dependent on the amount of antibody mass in the range of 69 to 340 ng. The positive detection rate and the negative detection rate were 80% and 100%, respectively.
  
  
  CONCLUSIONS
  This immunoassay provides a viable alternative to other early ovarian cancer detection methods and is particularly suited for health screening of the general population.
authors.affiliation: School of Automation and Engineering, University of Electronic Science and Technology of China, Chengdu, China. yanchencd@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Xian-He
authors.initials: XH
authors.lastname: Huang
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Shi
authors.forename: Bo
authors.initials: B
authors.lastname: Mu
authors.forename: Qun
authors.initials: Q
authors.lastname: Lv
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D045744:Cell Line, Tumor; D005260:Female; D007106:Immune Sera / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods; D010051:Ovarian Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods; D011817:Rabbits; D051381:Rats
chemical_list: D000906:Antibodies; D007106:Immune Sera