title: [Pharmacokinetic of four alkaloids of Yanshu injection in Beagel dogs].
abstract: OBJECTIVE
  For studying the pharmacokinetic of Yanshu injections in Beagel dogs, a sensitive and reproducible LC-MS method for quantitative determination of matrine, oxymatrine, sophocarpine and oxysophocarpine in dog's plasma were developed and validated using monocrotaline as an internal standard after iv of Yanshu injections (Sophorae Flavescentis Radix and Heterosmilacis Japonicae Rhizoma).
  
  
  METHODS
  The separation of plasma samples was performed on a CN column by isocratic elution with methanol-10 mmol x L(-1) NH4Ac-0.02% HCOOH-H2O 90:10 as the mobile phase. The plasma concentration of four kinds of alkaloids were calculated in dog plasta by detection of healthy dogs given Yanshu injection fluid after in twelve hours of plasma samples, All data of concentration-time of four kinds of alkaloids were treated with pharmacokinetics program DAS 2. 0.
  
  
  RESULTS
  MT, OMT, SP and OSP have a good linear relationship in 0.01-16.0, 0.02-60.0, 0.01-4.0, 0.02-16.0 mg x L(-1), respectively. The average recoveries were more than 90% and the RSD of precision and stability of the test were less than 6.4% iv 1.2 g x kg(-1) Yanshu injection, four kinds of alkaloids in rats meet the two-compartment open pharmacokinetic model, Cmax and the concentration of the original liquid in the proportion of the basic line, the AUC(0-infinity) of matrine and oxymatrine, sophocarpine and oxysophocarpine compared to the original both in the proportion of liquid increases, the MRT(0-infinity) and t(1/2z) of matrine and sophocarpine were less than oxymatrine and oxysophocarpine; four kinds of alkaloids apparent volume of distribution matrine > oxymatrine, sophocarpine > oxysophocarpine.
  
  
  CONCLUSIONS
  A method with high recovery rate and good stabilitywas established to determine the blood concentration of MT, OMT, SP, OSP in Yanshu injection and applied in its pharmacokinetics successfully.
authors.affiliation: Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China. liwenjie780711@163.com
authors.forename: Jiping
authors.initials: J
authors.lastname: Liu
authors.forename: Mei
authors.initials: M
authors.lastname: Xue
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.forename: Zhenzhou
authors.initials: Z
authors.lastname: Jiang
authors.forename: Luyong
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000470:Alkaloids / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D002853:Chromatography, Liquid; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry*; D007267:Injections; D013058:Mass Spectrometry
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal