title: Analysis of salivary gland transcripts of the sand fly Lutzomyia ayacuchensis, a vector of Andean-type cutaneous leishmaniasis.
abstract: The saliva of blood sucking insects contains potent pharmacologically active components that assist them in counteracting the host hemostatic and inflammatory systems during blood feeding. In addition, sand fly salivary proteins affect host immunity and have the potential to be a vaccine against Leishmania infection. In the present study, the salivary gland transcripts of Lutzomyia ayacuchensis, a vector of cutaneous leishmaniasis in Ecuadorian and Peruvian Andes, were analyzed by sequencing randomly selected clones of the salivary gland cDNA library of this sand fly. This resulted in the identification of the most abundant transcripts coding for secreted proteins. These proteins were homologous to the salivary molecules present in other sand flies including the RGD-containing peptide, PpSP15/SL1 family protein, yellow-related protein, putative apyrase, antigen 5-related protein, D7 family protein, and 27 kDa salivary protein. Of note, homologues of maxadilan, an active vasodilator abundantly present in saliva of Lutzomyia longipalpis, were not identified. This analysis is the first description of salivary proteins from a sand fly of the subgenus Helcocyrtomyia and from vector of cutaneous leishmaniasis in the New World. The present analysis will provide further insights into the evolution of salivary components in blood sucking arthropods.
authors.affiliation: Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Japan. hkato@vetmed.hokudai.ac.jp
authors.forename: Hirotomo
authors.initials: H
authors.lastname: Kato
authors.forename: Ryan C
authors.initials: RC
authors.lastname: Jochim
authors.forename: Eduardo A
authors.initials: EA
authors.lastname: Gomez
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uezato
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Mimori
authors.forename: Masataka
authors.initials: M
authors.lastname: Korenaga
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Sakurai
authors.forename: Ken
authors.initials: K
authors.lastname: Katakura
authors.forename: Jesus G
authors.initials: JG
authors.lastname: Valenzuela
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hashiguchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015723:Gene Library; D020043:Genes, Essential; D059014:High-Throughput Nucleotide Sequencing; D016773:Leishmaniasis, Cutaneous / Q000635:transmission; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D011576:Psychodidae / Q000235:genetics*; D012469:Salivary Glands / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D059467:Transcriptome
chemical_list: D012471:Salivary Proteins and Peptides