title: Effects of sterilization with hydrogen peroxide gas plasma, ethylene oxide, and steam on bioadhesive properties of nylon and polyethylene lines used for stabilization of canine stifle joints.
abstract: OBJECTIVE
  To compare effects of sterilization with hydrogen peroxide gas plasma (HPGP), ethylene oxide, and steam on bioadhesive properties of nylon and polyethylene lines used for stabilization of canine stifle joints.
  
  
  METHODS
  Samples of a 36.3-kg test nylon leader line, 57.8-kg test nylon fishing line, and 2-mm ultrahigh-molecular weight polyethylene (UHMWPE) were used.
  
  
  METHODS
  In this in vitro study, samples of nylon leader line, fishing line, and UHMWPE sterilized by use of HPGP, ethylene oxide, and steam or unsterilized samples were used. Bacterial adherence on unsterilized and sterilized samples was tested with Staphylococcus epidermidis and Escherichia coli. Five samples were examined for each line type and sterilization condition, and final colony counts were obtained.
  
  
  RESULTS
  Bacterial adherence was significantly affected by method of sterilization for all 3 line types. For most of the samples, bacterial adherence was similar or lower when HPGP sterilization was used, compared with results for sterilization via ethylene oxide and steam, respectively. Bacterial adherence was significantly higher for UHMWPE, compared with adherence for the nylon line, regardless of the sterilization method used. Bacterial adherence was higher for nylon fishing line than for nylon leader line for S epidermidis after ethylene oxide sterilization and for E coli after HPGP and ethylene oxide sterilization.
  
  
  CONCLUSIONS
  Effects of HPGP sterilization on bioadhesive properties of nylon and polyethylene lines compared favorably with those for ethylene oxide and steam sterilization. Also, nylon line may be a more suitable material than UHMWPE for suture prostheses on the basis of bacterial adherence properties.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, QC J2S 2M2, Canada.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Gatineau
authors.forename: Alexander O
authors.initials: AO
authors.lastname: El-Warrak
authors.forename: Christian
authors.initials: C
authors.lastname: Bolliger
authors.forename: Michael
authors.initials: M
authors.lastname: Mourez
authors.forename: Frederic
authors.initials: F
authors.lastname: Berthiaume
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D004283:Dog Diseases / Q000601:surgery; D004285:Dogs / Q000601:surgery; D004926:Escherichia coli / Q000254:growth & development / Q000502:physiology; D005027:Ethylene Oxide / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry; D007593:Joint Instability / Q000601:surgery / Q000662:veterinary; D008422:Materials Testing / Q000662:veterinary; D009757:Nylons / Q000737:chemistry*; D058626:Plasma Gases / Q000737:chemistry; D011095:Polyethylenes / Q000737:chemistry*; D013212:Staphylococcus epidermidis / Q000254:growth & development / Q000502:physiology; D013227:Steam / Q000032:analysis; D013242:Sterilization / Q000379:methods*; D013264:Stifle / Q000601:surgery*; D013536:Suture Techniques / Q000662:veterinary; D013537:Sutures / Q000662:veterinary*
chemical_list: D009757:Nylons; D058626:Plasma Gases; D011095:Polyethylenes; D013227:Steam; C111601:ultra-high molecular weight polyethylene; D006861:Hydrogen Peroxide; D005027:Ethylene Oxide