title: Quantification of sparfloxacin in pharmaceutical dosages and biological samples.
abstract: A simple and fast method for spectrophotometric determination of sparfloxacin using p-dimethyl-aminobenzaldehyde (DMAB) has been developed.  A yellow coloured product formed from reaction between sparfloxacin and DMAB as a result of condensation reaction at room temperature. The maximum absorbance was found at 392 nm with molar absorptivity of 4.9 × 10(3) L mol(-1) cm(-1). All parameters for the reaction, as concentration of DMBA reagent, molarity of sulphuric acid, and reaction temperature were studied. Under the conditions studied, a linear relationship between absorbance of the condensation product and concentration of sparfloxacin in the range of 2.0-80.0 μg mL(-1) was found with good correlation coefficient (0.9997). The limits of detection (LOD) and quantification (LOQ) for the proposed method were found to be 0.22 and 0.75 μg mL(-1) respectively. The repeatability and accuracy (model) of the method was studied at three different concentrations of sparfloxacin and found with value of relative standard deviation less than 2.0%. The method was found selective for determination of sparfloxacin in the presence of commonly used excipients in dosage forms. The developed method was validated statistically and applied successfully to the analysis of the drug in pure form, pharmaceutical preparations, and spiked blood plasma and urine samples with good accuracy (real) and precision. The percentage recovery was found from 99.0-100.0% with relative standard deviation less than 1%. The results of the proposed method were compared statistically with the results of literature HPLC method.
authors.affiliation: Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan. jasminshah2001@yahoo.com
authors.forename: Jasmin
authors.initials: J
authors.lastname: Shah
authors.forename: Muhammad Rasul
authors.initials: MR
authors.lastname: Jan
authors.forename: Inayatullah
authors.initials: I
authors.lastname: Khan
authors.forename: Muhammad Naeem
authors.initials: MN
authors.lastname: Khan
mesh_terms: D000995:Antitubercular Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D001547:Benzaldehydes / Q000737:chemistry; D002626:Chemistry, Pharmaceutical; D004304:Dosage Forms; D024841:Fluoroquinolones / Q000032:analysis* / Q000097:blood / Q000652:urine; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods* / Q000592:standards; D013696:Temperature
chemical_list: D000995:Antitubercular Agents; D001547:Benzaldehydes; D004304:Dosage Forms; D024841:Fluoroquinolones; C061363:sparfloxacin; C510241:p-dimethylaminobenzaldehyde