title: Ethanol extract of peanut sprout induces Nrf2 activation and expression of antioxidant and detoxifying enzymes in human dermal fibroblasts: implication for its protection against UVB-irradiated oxidative stress.
abstract: A peanut sprout is known to contain a significant level of resveratrol, which was reported to have beneficial effects in our body due to its antioxidant activities. The purpose of this study was to evaluate the cytoprotective activity of ethanol extract of peanut sprout (EPS) from ultraviolet B (UVB)-induced oxidative stress in human dermal fibroblasts (HDF). EPS was revealed to contain 54.2 μg g(-1) of trans-resveratrol. The DCF-DA-positive reactive oxygen species level was increased by 50 mJ cm(-2) of UVB irradiation (2150 ± 450% of nonirradiated control), which was markedly suppressed by EPS treatment (180 ± 42% of control). Annexin V-positive apoptotic cell death induced by UVB irradiation (16.4 ± 4.5%) was also significantly inhibited by EPS treatment (6.7 ± 2.5%). EPS induced up-regulation and nuclear translocation of Nrf2, a transcription factor for antioxidant and detoxifying enzymes, in HDF as a dose-dependent manner. UVB irradiation up-regulated Nrf2-dependent enzymes of heme oxygenase-1, NAD(P)H:quinine oxidoreductase-1 and glutathione-S-transferase pi, and they were further stimulated by EPS treatment. Taken together, EPS is an efficient cytoprotective agent against UVB-induced oxidative stress by activation of Nrf2 and upregulation of Nrf2-relating antioxidant and detoxifying enzymes in HDF.
authors.affiliation: Department of Dermatology of Chonnam National University Medical School, Chonnam National University, Gwangju, Korea.
authors.forename: Jee-Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Da-In
authors.initials: DI
authors.lastname: Choi
authors.forename: Jee-Bum
authors.initials: JB
authors.lastname: Lee
authors.forename: Suk-Jung
authors.initials: SJ
authors.lastname: Yun
authors.forename: Dong-Ho
authors.initials: DH
authors.lastname: Lee
authors.forename: Jong-Bang
authors.initials: JB
authors.lastname: Eun
authors.forename: Seung-Chul
authors.initials: SC
authors.lastname: Lee
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D010367:Arachis / Q000737:chemistry*; D020405:Dermis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D000431:Ethanol; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects* / Q000528:radiation effects; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D061251:Primary Cell Culture; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors; D000077185:Resveratrol; D036226:Seedlings / Q000737:chemistry; D013267:Stilbenes / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D013267:Stilbenes; D000431:Ethanol; D051547:Heme Oxygenase-1; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human; D005982:Glutathione Transferase; D000077185:Resveratrol