title: Proteomic identification of MYC2-dependent jasmonate-regulated proteins in Arabidopsis thaliana.
abstract: BACKGROUND
  MYC2, a basic helix-loop-helix (bHLH) domain-containing transcription factor, participates in the jasmonate (JA) signaling pathway and is involved in the modulation of diverse JA functions. However, a comprehensive list of MYC2-dependent JA-responsive proteins has yet to be defined.
  
  
  RESULTS
  In this paper, we report the comparative proteomics of wild-type (WT) plants and jin1-9, a MYC2 mutant plant, in response to methyl jasmonate (MeJA) treatment. Proteins from mock/MeJA-treated jin1-9 and WT samples were extracted and separated by two-dimensional gel electrophoresis. Twenty-seven JA-mediated proteins demonstrated differential expression modulated by MYC2. We observed that MYC2 negatively regulates the accumulation of JA-dependent indolic glucosinolate-related proteins and exhibits opposite effects on the biosynthetic enzymes involved aliphatic glucosinolate pathways. In addition, proteins involved in the tricarboxylic acid cycle and a majority of the MeJA-inducible proteins that are involved in multiple protective systems against oxidative stress were reduced in jin1-9/myc2 sample compared to the WT sample. These results support a positive role for MYC2 in regulating JA-mediated carbohydrate metabolism and oxidative stress tolerance.
  
  
  CONCLUSIONS
  We have identified MYC2-dependent jasmonate-regulated proteins in Arabidopsis thaliana by performing two-dimensional gel electrophoresis and MALDI-TOF/TOF MS analysis. The observed pattern of protein expression suggests that MYC2 has opposite effects on the biosynthetic enzymes of indolic and aliphatic glucosinolate pathways and positively regulates JA-mediated carbohydrate metabolism and oxidative stress tolerance-related proteins. Furthermore, it is very interesting to note that MYC2 plays opposite roles in the modulation of a subset of JA-regulated photosynthetic proteins during short-term and long-term JA signaling. This study will enhance our understanding of the function of MYC2 in JA signaling in Arabidopsis thaliana.
authors.affiliation: College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China.|Alkali Soil Natural Environmental Science Center; Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China.|Alkali Soil Natural Environmental Science Center; Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Northeast Forestry University, Harbin, 150040, China.
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Pang
authors.affiliation: College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.affiliation: College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Yan