title: Angiotensin II induced differentially expressed microRNAs in adult rat cardiac fibroblasts.
abstract: Angiotensin II (Ang II) plays a pivotal role in cardiac fibrosis, and microRNAs (miRNAs) have been shown to participate in diverse pathological processes. Our aim is to identify the Ang II-induced miRNAs in cardiac fibroblasts (CFs). The miRNA array was used to analyze the miRNA expression profile in CFs treated by Ang II and control cells. Stem-loop real-time PCR was performed to re-measure the levels of the differentially expressed miRNAs. Analysis of miRNA arrays showed that 33 miRNAs were differentially expressed (13 up- and 20 downregulated) in response to Ang II (100 nM) for 24 h as compared to control cells. Quantitative PCR revealed that Ang II upregulated the levels of miR-132, -125b-3p and miR-146b but downregulated the levels of miR-300-5p, -204* and miR-181b in CFs. The trend of miRNA change is consistent with microarray and qRT-PCR. Bioinformatic analysis revealed that MMP9 as the target of miR-132, MMP16 as the target of miR-146b and TIMP3 as the target of miR-181b have been listed in the miR database with experimentally validated targets, indicating the potential role of those miRNAs in cardiac fibrosis. Our results demonstrated that we did identify a subset of miRNAs that was differentially expressed in Ang II-treated CFs, which provide a starting point to explore their potential roles in cardiac fibrosis and hypertension.
authors.affiliation: Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, 76 Yanta West Road, Xi'an, 710061, Shaanxi, China. jiangxy@mail.xjtu.edu.cn.|NIHR Translational Research Facility in Respiratory Medicine Group, School of Translational Medicine, Stopford Building, University of Manchester, Manchester, M13 9PT, UK. jiangxy@mail.xjtu.edu.cn.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, 76 Yanta West Road, Xi'an, 710061, Shaanxi, China.
authors.forename: Qilan
authors.initials: Q
authors.lastname: Ning
authors.affiliation: Department of Genetics and Molecular Biology, School of Medicine, Xi'an Jiaotong University, 76 Yanta West Road, Xi'an, 710061, Shaanxi, China.
authors.forename: Juanli
authors.initials: J
authors.lastname: Wang
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D019295:Computational Biology / Q000379:methods; D015536:Down-Regulation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D006973:Hypertension / Q000235:genetics / Q000378:metabolism; D008297:Male; D053513:Matrix Metalloproteinase 16 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D035683:MicroRNAs; D019717:Tissue Inhibitor of Metalloproteinase-3; D000804:Angiotensin II; D053513:Matrix Metalloproteinase 16; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat