title: Performance of different screening methods for the determination of urinary glycosaminoclycans.
abstract: BACKGROUND
  The study aim was to compare the performance of three different methods used for determining urinary glycosaminoglycans (GAG) levels in spot and 24-h urine samples.
  
  
  METHODS
  Performance characteristics were studied for cetylpyridinium chloride (CPC), and manual and automated dimethylmethylene blue (DMB) methods.
  
  
  RESULTS
  For automated DMB method, within-run precisions were 9.10% and 1.98%, and between-day precisions were 13.0% and 5.81% in low- and high-urine pools, respectively. The method was linear up to 100 mg/L of GAG concentration. The detection limit of the method was 0.71 mg/L. Mean recovery was 95.7%.
  
  
  CONCLUSIONS
  The automated DMB method was found to give better performance characteristics than cetylpyridinium chloride (CPC) and manual DMB methods. It is a fast, cheap, simple and reliable method and can be applied in many diseases in which GAG is used as a screening test.
authors.affiliation: Department of Medical Biochemistry, Ministry of Health, Ankara Training and Research Hospital, Ankara, Turkey.
authors.forename: Ebru
authors.initials: E
authors.lastname: Afyoncu
authors.forename: Gülsen
authors.initials: G
authors.lastname: Yilmaz
authors.forename: Fatma M
authors.initials: FM
authors.lastname: Yilmaz
authors.forename: Doğan
authors.initials: D
authors.lastname: Yücel
mesh_terms: D001172:Arthritis, Rheumatoid / Q000652:urine; D002594:Cetylpyridinium / Q000737:chemistry; D003124:Colorimetry / Q000379:methods; D006025:Glycosaminoglycans / Q000652:urine*; D006801:Humans; D008751:Methylene Blue / Q000031:analogs & derivatives / Q000737:chemistry; D009391:Nephelometry and Turbidimetry / Q000379:methods; D010003:Osteoarthritis / Q000652:urine; D016482:Urinalysis / Q000379:methods*
chemical_list: D006025:Glycosaminoglycans; C435946:dimethylmethylene blue; D002594:Cetylpyridinium; D008751:Methylene Blue