title: Microfluidic chips with reversed-phase monoliths for solid phase extraction and on-chip labeling.
abstract: The integration of sample preparation methods into microfluidic devices provides automation necessary for achieving complete micro total analysis systems. We have developed a technique that combines on-chip sample enrichment with fluorescence labeling and purification. Polymer monoliths made from butyl methacrylate were fabricated in cyclic olefin copolymer microdevices and used for solid phase extraction. We studied the retention of fluorophores, amino acids and proteins on these columns. The retained samples were subsequently labeled with both Alexa Fluor 488 and Chromeo P503, and unreacted dye was rinsed off the column before sample elution. Additional purification was obtained from the differential retention of proteins and fluorescent labels. A linear relation between the eluted peak areas and concentrations of on-chip labeled heat shock protein 90 samples demonstrated the utility of this method for on-chip quantitation. Our fast and simple method of simultaneously concentrating and labeling samples on-chip is compatible with miniaturization and desirable for automated analysis.
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors.forename: Pamela N
authors.initials: PN
authors.lastname: Nge
authors.forename: Jayson V
authors.initials: JV
authors.lastname: Pagaduan
authors.forename: Ming
authors.initials: M
authors.lastname: Yu
authors.forename: Adam T
authors.initials: AT
authors.lastname: Woolley
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D002417:Cattle; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation* / Q000379:methods; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D005456:Fluorescent Dyes / Q000302:isolation & purification; D008689:Methacrylates / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000295:instrumentation* / Q000379:methods
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D008689:Methacrylates; D011506:Proteins; C016284:butyl methacrylate