title: Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times.
abstract: The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation. Histopathological changes in the heart, liver, and stomach indicated that these organs sustained different degrees of injury. Hsp70 protein expression in the heart and liver of transported pigs did not change significantly while it increased significantly (p < 0.05) in the stomach. Hsp70 mRNA levels decreased significantly (p < 0.05) in the heart after 4 h of transportation. However, mRNA expression increased significantly in the liver after 1 (p < 0.05) and 4 h (p < 0.01) of transportation, and increased significantly in the stomach of the transported pigs after 1, 4 (p < 0.01), and 2 h (p < 0.05). HSF-1 levels were reduced at 1 and 4 h (p < 0.05) only in the hearts of transported pigs. These results indicate that Hsp70 mediates distinct stress-related functions in different tissues during transportation.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Yue
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Liu
authors.forename: Ali
authors.initials: A
authors.lastname: Islam
authors.forename: Buriro
authors.initials: B
authors.lastname: Rehana
authors.forename: Shu
authors.initials: S
authors.lastname: Tang
authors.forename: Endong
authors.initials: E
authors.lastname: Bao
authors.forename: Jörg
authors.initials: J
authors.lastname: Hartung
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000097:blood; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005753:Gastric Mucosa / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D000076249:Heat Shock Transcription Factors; D008099:Liver / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013312:Stress, Physiological; D013552:Swine / Q000097:blood / Q000378:metabolism*; D013997:Time Factors; D000637:Transaminases / Q000097:blood; D014157:Transcription Factors / Q000378:metabolism*; D014186:Transportation
chemical_list: D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D012333:RNA, Messenger; D014157:Transcription Factors; D000637:Transaminases; D003402:Creatine Kinase