title: Cytotoxicity evaluation of vancomycin and its complex with beta-cyclodextrin on human glial cell line.
abstract: The possible cytotoxic effects of vancomycin and its complex with beta-cyclodextrin (β-CD) on human glial cell line (CRL 8621) were studied accordingly by means of MTS assay. The cultured cells were incubated with various concentrations of vancomycin, β-CD as well as β-CD/vancomycin complex ranging from 4.69 to 300 ug/ml. A linear dose-dependency cytotoxicity followed by hermetic-like biphasic dose-dependence was observed after incubation period of 72 hours. In general, significant increase (p<0.001) of cell proliferation was observed at lower concentrations: <18.75 μg/ml for cells treated with β-CD and their complex while < 9.38 μg/ml for cells treated with vancomycin. In contrary, regardless of the treatments given, significant (p<0.001) reduce in cell survival was found at higher concentrations >150 μg/ml. In particular, 50 % inhibitory in vitro was achieved at the concentrations of 115.95 μg/ml (for β-CD), 116.48 μg/ml (for vancomycin) and 115.44 μg/ml (for β-CD/vancomycin complex).
authors.affiliation: Department of Neurosciences, Universiti Sains Malaysia, Kelantan, Malaysia. zarifsofian@gmail.com
authors.forename: Zarif Mohamed
authors.initials: ZM
authors.lastname: Sofian
authors.forename: Jafri Malin
authors.initials: JM
authors.lastname: Abdullah
authors.forename: Afidah Abdul
authors.initials: AA
authors.lastname: Rahim
authors.forename: Shazrin Shazira
authors.initials: SS
authors.lastname: Shafee
authors.forename: Zulkifli
authors.initials: Z
authors.lastname: Mustafa
authors.forename: Shariza Abdul
authors.initials: SA
authors.lastname: Razak
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D006801:Humans; D020128:Inhibitory Concentration 50; D009457:Neuroglia / Q000187:drug effects* / Q000473:pathology; D014640:Vancomycin / Q000737:chemistry / Q000633:toxicity*; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000633:toxicity*
chemical_list: D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D047392:beta-Cyclodextrins; D014640:Vancomycin