title: Screening of antifungal azole drugs and agrochemicals with an adapted alamarBlue-based assay demonstrates antibacterial activity of croconazole against Mycobacterium ulcerans.
abstract: An alamarBlue-based growth inhibition assay has been adapted for the thermosensitive and slow-growing pathogen Mycobacterium ulcerans. The standardized test procedure enables medium-throughput screening of preselected compound libraries. Testing of a set of 48 azoles with known antifungal activity led to the identification of an imidazole antifungal displaying an inhibitory dose (ID) of 9 μM for M. ulcerans.
authors.affiliation: Swiss Tropical and Public Health Institute, Basel, Switzerland. nicole.scherr@unibas.ch
authors.forename: Nicole
authors.initials: N
authors.lastname: Scherr
authors.forename: Katharina
authors.initials: K
authors.lastname: Röltgen
authors.forename: Matthias
authors.initials: M
authors.lastname: Witschel
authors.forename: Gerd
authors.initials: G
authors.lastname: Pluschke
mesh_terms: D016573:Agrochemicals / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology*; D004396:Coloring Agents; D057166:High-Throughput Screening Assays; D007093:Imidazoles / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D019911:Mycobacterium ulcerans / Q000187:drug effects*; D010078:Oxazines; D014966:Xanthenes
chemical_list: D016573:Agrochemicals; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001393:Azoles; D004396:Coloring Agents; D007093:Imidazoles; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; C038008:croconazole