title: Proteomic analysis of the mice hippocampus after preconditioning induced by N-methyl-D-aspartate (NMDA).
abstract: Preconditioning induced by N-methyl-D-aspartate (NMDA) has been used as a therapeutic tool against later neuronal insults. NMDA preconditioning affords neuroprotection against convulsions and cellular damage induced by the NMDA receptor agonist, quinolinic acid (QA) with time-window dependence. This study aimed to evaluate the molecular alterations promoted by NMDA and to compare these alterations in different periods of time that are related to the presence or lack of neuroprotection. Putative mechanisms related to NMDA preconditioning were evaluated via a proteomic analysis by using a time-window study. After a subconvulsant and protective dose of NMDA administration mice, hippocampi were removed (1, 24 or 72 h) and total protein analyzed by 2DE gels and identified by MALDI-TOF. Differential protein expression among the time induction of NMDA preconditioning was observed. In the hippocampus of protected mice (24 h), four proteins: HSP70(B), aspartyl-tRNA synthetase, phosphatidylethanolamine binding protein and creatine kinase were found to be up-regulated. Two other proteins, HSP70(A) and V-type proton ATPase were found down-regulated. Proteomic analysis showed that the neuroprotection induced by NMDA preconditioning altered signaling pathways, cell energy maintenance and protein synthesis and processing. These events may occur in a sense to attenuate the excitotoxicity process during the activation of neuroprotection promoted by NMDA preconditioning.
authors.affiliation: Centro de Biologia Molecular Estrutural, Departamento de Bioquímica, Universidade Federal de Santa Catarina, CEP 88040-900, Florianópolis, SC, Brazil.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: do Amaral e Silva Müller
authors.forename: Samuel
authors.initials: S
authors.lastname: Vandresen-Filho
authors.forename: Carolina Pereira
authors.initials: CP
authors.lastname: Tavares
authors.forename: Angela C O
authors.initials: AC
authors.lastname: Menegatti
authors.forename: Hernán
authors.initials: H
authors.lastname: Terenzi
authors.forename: Carla Inês
authors.initials: CI
authors.lastname: Tasca
authors.forename: Patricia Cardoso
authors.initials: PC
authors.lastname: Severino
mesh_terms: D000818:Animals; D001226:Aspartate-tRNA Ligase / Q000235:genetics / Q000378:metabolism; D003402:Creatine Kinase / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D016202:N-Methylaspartate / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018840:HSP70 Heat-Shock Proteins; D018696:Neuroprotective Agents; D049488:Phosphatidylethanolamine Binding Protein; D016202:N-Methylaspartate; D003402:Creatine Kinase; D001226:Aspartate-tRNA Ligase