title: Small nucleolar RNA expression profiling identifies potential prognostic markers in peripheral T-cell lymphoma.
abstract: Peripheral T-cell lymphoma (PTCL) is a rare, heterogeneous type of non-Hodgkin lymphoma (NHL) that, in general, is associated with a poor clinical outcome. Therefore, a current major challenge is the discovery of new prognostic tools for this disease. In the present study, a cohort of 122 patients with PTCL was collected from a multicentric T-cell lymphoma consortium (TENOMIC). We analyzed the expression of 80 small nucleolar RNAs (snoRNAs) using high-throughput quantitative PCR. We demonstrate that snoRNA expression analysis may be useful in both the diagnosis of some subtypes of PTCL and the prognostication of both PTCL-not otherwise specified (PTCL-NOS; n = 26) and angio-immunoblastic T-cell lymphoma (AITL; n = 46) patients treated with chemotherapy. Like miRNAs, snoRNAs are globally down-regulated in tumor cells compared with their normal counterparts. In the present study, the snoRNA signature was robust enough to differentiate anaplastic large cell lymphoma (n = 32) from other PTCLs. For PTCL-NOS and AITL, we obtained 2 distinct prognostic signatures with a reduced set of 3 genes. Of particular interest was the prognostic value of HBII-239 snoRNA, which was significantly over-expressed in cases of AITL and PTCL-NOS that had favorable outcomes. Our results suggest that snoRNA expression profiles may have a diagnostic and prognostic significance for PTCL, offering new tools for patient care and follow-up.
authors.affiliation: Cancer Research Center of Toulouse, Toulouse, France.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Valleron
authors.forename: Loic
authors.initials: L
authors.lastname: Ysebaert
authors.forename: Laure
authors.initials: L
authors.lastname: Berquet
authors.forename: Virginie
authors.initials: V
authors.lastname: Fataccioli
authors.forename: Cathy
authors.initials: C
authors.lastname: Quelen
authors.forename: Antoine
authors.initials: A
authors.lastname: Martin
authors.forename: Marie
authors.initials: M
authors.lastname: Parrens
authors.forename: Laurence
authors.initials: L
authors.lastname: Lamant
authors.forename: Laurence
authors.initials: L
authors.lastname: de Leval
authors.forename: Christian
authors.initials: C
authors.lastname: Gisselbrecht
authors.forename: Philippe
authors.initials: P
authors.lastname: Gaulard
authors.forename: Pierre
authors.initials: P
authors.lastname: Brousset
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016411:Lymphoma, T-Cell, Peripheral / Q000175:diagnosis* / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D020537:RNA, Small Nucleolar / Q000235:genetics*
chemical_list: D020537:RNA, Small Nucleolar