title: Treadmill rehabilitation treatment enhanced BDNF-TrkB but not NGF-TrkA signaling in a mouse intracerebral hemorrhage model.
abstract: Intracerebral hemorrhage (ICH), accounting for 15-20% of strokes, can cause significant brain injury and life long neurological deficits. We investigated whether treadmill exercise rehabilitation could improve brain repair after ICH and whether involvement of NFG-TrkA and BDNF-TrkB signaling could be observed during repair period in an experimental mouse ICH model reproduced by heparinized-collagenase infusion into the left caudate putamen. 5-Bromo-2-deoxyuridine (BrdU) labeled new dividing cell can be observed clearly around the injured cortex and striatum region on day 7 (D7) after operation, and both TrkA and TrkB neurotropic receptors were activated. A subgroup of these ICH mice began the treadmill exercise from D4 after operation. Then we found that the overall immunofluorescent signals of p-Y490-TrkA and p-Y705-TrkB were both decreased in all groups at D14 after operation. However, compared to the non-exercise ICH group mouse, the immunofluorescent intensity of BDNF and p-Y705-TrkB were significantly higher in the exercise group. In addition, there was no difference in p-Y490-TrkA. Our results suggest that BDNF-TrkB but not NGF-TrkA signaling is involved in the brain repair after ICH, and early proper treadmill exercise might promote this repair process.
authors.affiliation: Institute of Nutrition, Chinese Academy of Sciences, Shanghai 200031, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Qin
authors.forename: Qian
authors.initials: Q
authors.lastname: Su
authors.forename: Zhixue
authors.initials: Z
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000150:complications / Q000378:metabolism* / Q000534:rehabilitation*; D005081:Exercise Therapy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020932:Nerve Growth Factor / Q000378:metabolism*; D009416:Nerve Regeneration / Q000502:physiology; D020813:Receptor, trkB / Q000378:metabolism; D015398:Signal Transduction; D020521:Stroke / Q000150:complications / Q000378:metabolism*; D000071939:Stroke Rehabilitation; D016896:Treatment Outcome
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020932:Nerve Growth Factor; D020813:Receptor, trkB