title: Evaluation of topical photodynamic therapy of mammary carcinoma with an experimental gel containing liposomal hydroxyl-aluminium phthalocyanine.
abstract: BACKGROUND
  Photodynamic therapy (PDT) is a clinically-accepted approach for the therapy of many types of cancer. This study focused on the treatment of mammarian carcinoma by topical administration of hydroxyl-aluminium phthalocyanine (AlOH-PC), compared to a clinically-approved photosensitizer (Metvix, Galderma & PhotoCure ASA, Inc., Oslo, Norway).
  
  
  METHODS
  MDA-MB 231 cells were subcutaneously injected into the right flank of athymic nude mice. Mice with grown tumours were used for in vivo efficacy studies. Different doses of liposomal AlOH-PC were applied to determine the most effective dose. In later studies, Metvix or our liposomal-AlOH-PC gel formula were used. Topical application of photosensitizers was followed by the PDT irradiation at 600-700 nm (635 nm peak). Tumour growth was measured three times weekly.
  
  
  RESULTS
  Therapeutic studies revealed that AlOH-PC treatment led to complete tumour remission in 90% (9/10) of experimental animals, whereas usage of the commercially available Metvix only postponed the tumour growth. Moreover, usage of liposomal AlOH-PC shortened the time allowed between the application of the photosensitizer and light exposure: for Metvix, hours are usually needed, while the tested liposomal AlOH-PC showed remarkable outcomes after only 10 min.
  
  
  CONCLUSIONS
  Liposomal AlOH-PC gel appears to be potentially suitable for PDT of mammarian carcinoma.
authors.affiliation: Clinic of Surgery, First Faculty of Medicine, Charles University and Thomayer Hospital, Prague, Czech Republic. sutoris@gmail.com
authors.forename: Karol
authors.initials: K
authors.lastname: Sutoris
authors.forename: David
authors.initials: D
authors.lastname: Vetvicka
authors.forename: Ladislav
authors.initials: L
authors.lastname: Horak
authors.forename: Jiri
authors.initials: J
authors.lastname: Benes
authors.forename: Milos
authors.initials: M
authors.lastname: Nekvasil
authors.forename: Petr
authors.initials: P
authors.lastname: Jezek
authors.forename: Marie
authors.initials: M
authors.lastname: Zadinova
authors.forename: Pavla
authors.initials: P
authors.lastname: Pouckova
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy*; D000818:Animals; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000008:administration & dosage* / Q000737:chemistry; D005260:Female; D005782:Gels; D006801:Humans; D007211:Indoles / Q000008:administration & dosage* / Q000737:chemistry; D008081:Liposomes; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000737:chemistry; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage* / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D056831:Coordination Complexes; D005782:Gels; D007211:Indoles; D008081:Liposomes; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; C060570:aluminum phthalocyanine