title: Comparative study of dye-loaded liposome accumulation in sensitive and resistant human breast cancer cells.
abstract: The aim of this research is to study the dynamics and efficiency of liposome accumulation in sensitive and resistant human breast cancer cells.
  
  
  METHODS
  Methods of fluorescence microscopy, fluorescence microspectroscopy and MTT-test have been used.
  
  
  RESULTS
  The liposome-to-cell interaction and dye cellular uptake in sensitive, cisplatin-resistant and doxorubicin-resistant MCF-7 human breast cancer cells have been analyzed using time changes in both fluorescence resonance energy transfer signal from the donor probe DiO to the acceptor one DiI preloaded in liposomes and cell image brightness.
  
  
  CONCLUSIONS
  Obtained results show that resistant cells accumulate dye-loaded liposomes more effectively and reveal more effective dye molecule cellular uptake.
authors.affiliation: Institute for Scintillation Materials, NAS of Ukraine, Kharkov 61001, Ukraine. ephimova@isma.kharkov.ua
authors.forename: S L
authors.initials: SL
authors.lastname: Yefimova
authors.forename: I Yu
authors.initials: IY
authors.lastname: Kurilchenko
authors.forename: T N
authors.initials: TN
authors.lastname: Tkacheva
authors.forename: V A
authors.initials: VA
authors.lastname: Rozhkov
authors.forename: A V
authors.initials: AV
authors.lastname: Sorokin
authors.forename: N Yu
authors.initials: NY
authors.lastname: Lukianova
authors.forename: N A
authors.initials: NA
authors.lastname: Bezdenezhnykh
authors.forename: Yu V
authors.initials: YV
authors.lastname: Malyukin
authors.forename: V F
authors.initials: VF
authors.lastname: Chekhun
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism*; D002232:Carbocyanines / Q000737:chemistry / Q000493:pharmacokinetics; D002945:Cisplatin / Q000008:administration & dosage / Q000494:pharmacology; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics*; D019008:Drug Resistance, Neoplasm; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics*; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D010713:Phosphatidylcholines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution
chemical_list: D000970:Antineoplastic Agents; D002232:Carbocyanines; D004337:Drug Carriers; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; C024286:3,3'-dioctadecylindocarbocyanine; C098044:3,3'-dioctadecyloxacarbocyanine; D004317:Doxorubicin; D002945:Cisplatin