title: [Comparison of effect of formulas clearing away heat and promoting blood circulation on prevention and treatment of liver fibrosis in CCl4 mice].
abstract: OBJECTIVE
  To observe the effect of traditional Chinese medicine formulas clearing away heat and promoting blood circulation-Biejiayinzi (BJYZ), Gexiazhuyu Tang (GXZYT) and Fugan Wan (FGW) on liver fibrosis in CCl4 mice by screening and analyzing formula-syndrome database of kidney and liver fibrosis based on the principle of formula-syndrome, compared with pivot-harmonizing decoction.
  
  
  METHODS
  Ten-week-old male C57BL/6 mice, with the weight of (20 +/- 3) g, were randomly divided into 6 groups: the normal group, the model group, the BJYZ group, the GXZY group, the FGW group and the XST group. Except the normal group, other groups were abdominally injected with 10% CCl4 olive oil solution a dose of 2 mL x kg(-1) body weight for four weeks, three times each week. Meanwhile, the latter four groups were administered with extracts of BJYZ, GXZYT, FGW and XST, respectively, once every day, concomitantly continued CCl4 administration. The normal and the model groups were given the same volume of deionized water. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (gamma-GT), Alb and TBil were detected by chemiluminescence. The hydroxyproline (HYP) content was detected by acid hydrolysis method. The hepatic collagen deposition was evaluated with Sirius red staining.
  
  
  RESULTS
  Compared with the normal group, the model group recorded notable decrease in weight and increase in the ratio of liver weight and body weight and the ratio of spleen weight and body weight, with obvious fatty degeneration and inflammatory necrosis in liver cells. Collagen fiber deposition was so notable to form fibrous septums and pseudolobules. The levels of serum ALT, AST, TBil, gamma-GT, the HYP content in liver tissue and the deposition of hepatic collagen were significantly increased in the model group. Compared with model group, Serum AST were significantly decreased in BJYZ group as gamma-GT decreased in the GXZYT group, without notable decrease in degeneration and inflammatory necrosis in liver cells and collagen deposition. The GXZYT group showed significant decrease in gamma-GT, with slight improvement in degeneration and inflammatory necrosis in liver cells and reduction in collagen deposition. The ratio of liver weight and body weight, AST, gamma-GT and HYP content were significantly decreased in the FGW group, with slight improvement in degeneration and inflammatory necrosis in liver cells and reduction in collagen deposition. The XST group showed decrease in the ratio of liver weight and body weight, with no obvious change in inflammation and fibrosis of hepatic tissue.
  
  
  CONCLUSIONS
  FGW shows the best effect of prevention and treatment of liver fibrosis in CCl4 mice.
authors.affiliation: Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Liver Disease Research Institute of Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Zhao
authors.forename: Huiying
authors.initials: H
authors.lastname: Yu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Peng
authors.forename: Hongxu
authors.initials: H
authors.lastname: Ren
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Tao
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Wang
authors.forename: Bangxian
authors.initials: B
authors.lastname: Zhu
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Chenghai
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D001775:Blood Circulation / Q000187:drug effects*; D001831:Body Temperature / Q000187:drug effects*; D002251:Carbon Tetrachloride / Q000009:adverse effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D006909:Hydroxyproline / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009929:Organ Size / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D002251:Carbon Tetrachloride; D006909:Hydroxyproline