title: In vitro evaluation of the fermentation properties and potential prebiotic activity of caprine cheese whey oligosaccharides in batch culture systems.
abstract: The prebiotic effect of oligosaccharides recovered and purified from caprine whey, was evaluated by in vitro fermentation under anaerobic conditions using batch cultures at 37°C with human faeces. Effects on key gut bacterial groups were monitored over 24 h by fluorescence in situ hybridization (FISH), which was used to determine a quantitative prebiotic index score. Production of short-chain fatty acids (SCFAs) as fermentation end products was analyzed by high-performance liquid chromatography (HPLC). Growth of Bifidobacterium spp was significantly higher (P ≥ 0.05) with the purified oligosaccharides compared to the negative control. Lactic and propionic acids were the main SCFAs produced. Antimicrobial activity of the oligosaccharides was also tested, revealing no inhibition though a decrease in Staphylococcus aureus and Escherichia coli growth. These findings indicate that naturally extracted oligosaccharides from caprine whey could be used as new and valuable source of prebiotics.
authors.affiliation: Department of Food and Nutritional Sciences, University of Reading, Reading RG6 6AP, UK.
authors.forename: Diana L
authors.initials: DL
authors.lastname: Oliveira
authors.forename: Adele
authors.initials: A
authors.lastname: Costabile
authors.forename: R Andrew
authors.initials: RA
authors.lastname: Wilbey
authors.forename: Alistair S
authors.initials: AS
authors.lastname: Grandison
authors.forename: Luis C
authors.initials: LC
authors.lastname: Duarte
authors.forename: Luisa B
authors.initials: LB
authors.lastname: Roseiro
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques; D001644:Bifidobacterium / Q000254:growth & development; D002611:Cheese / Q000382:microbiology*; D003611:Dairy Products / Q000382:microbiology; D005232:Fatty Acids, Volatile / Q000737:chemistry; D005285:Fermentation; D041981:Gastrointestinal Tract / Q000382:microbiology; D006041:Goats; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009844:Oligosaccharides / Q000737:chemistry*; D056692:Prebiotics
chemical_list: D005232:Fatty Acids, Volatile; D009844:Oligosaccharides; D056692:Prebiotics