title: Designing industrial yeasts for the consolidated bioprocessing of starchy biomass to ethanol.
abstract: Consolidated bioprocessing (CBP), which integrates enzyme production, saccharification and fermentation into a one step process, is a promising strategy for the effective ethanol production from cheap lignocellulosic and starchy materials. CBP requires a highly engineered microbial strain able to both hydrolyze biomass with enzymes produced on its own and convert the resulting simple sugars into high-titer ethanol. Recently, heterologous production of cellulose and starch-degrading enzymes has been achieved in yeast hosts, which has realized direct processing of biomass to ethanol. However, essentially all efforts aimed at the efficient heterologous expression of saccharolytic enzymes in yeast have involved laboratory strains and much of this work has to be transferred to industrial yeasts that provide the fermentation capacity and robustness desired for large scale bioethanol production. Specifically, the development of an industrial CBP amylolytic yeast would allow the one-step processing of low-cost starchy substrates into ethanol. This article gives insight in the current knowledge and achievements on bioethanol production from starchy materials with industrial engineered S. cerevisiae strains.
authors.affiliation: Department of Agronomy Food Natural Resources Animals and Environment (DAFNAE), University of Padova, Agripolis, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Favaro
authors.forename: Tania
authors.initials: T
authors.lastname: Jooste
authors.forename: Marina
authors.initials: M
authors.lastname: Basaglia
authors.forename: Shaunita H
authors.initials: SH
authors.lastname: Rose
authors.forename: Maryna
authors.initials: M
authors.lastname: Saayman
authors.forename: Johann F
authors.initials: JF
authors.lastname: Görgens
authors.forename: Sergio
authors.initials: S
authors.lastname: Casella
authors.forename: Willem H
authors.initials: WH
authors.lastname: van Zyl
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*
chemical_list: D000431:Ethanol; D013213:Starch