title: Effect of ProNectin F derivatives on cell attachment and proliferation.
abstract: ProNectin F (PnF) was chemically modified by introducing some functional groups to prepare various derivatives of primary amino (PnF-N₁), tertiary amino (PnF-N₃), quaternary ammonium (PnF-N₄), carboxyl (PnF-COOH) and sulfonyl groups (PnF-SO₃H). When C3H10T1/2 cells were cultured on non-treated dishes coated with the derivatives, the number of mesenchymal cells attached to the culture dishes increased for the coating with PnF-COOH and PnF-SO₃H, even at their low adsorption amount. The cytotoxicity was high for the coating of PnF-N₁ and PnF-N₄ compared with that of the PnF-N₃, PnF-COOH and PnF-SO₃H. The treatment with integrin α5 and αV antibodies suppressed the cell attachment to the dishes coated with PnF-COOH and PnF-SO₃H. The phosphorylation of extracellular signal-regulated kinase (ERK) was upregulated for cells attached to the dishes coated with PnF-COOH and PnF-SO₃H, indicating their enhanced proliferation. It is concluded that the chemical derivatization of PnF enhanced the ability of cell attachment and proliferation.
authors.affiliation: Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Somamoto
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry / Q000494:pharmacology*; D016023:Integrins / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D016023:Integrins; D011994:Recombinant Proteins; C070367:ProNectin F; D003094:Collagen; D007770:L-Lactate Dehydrogenase; D048049:Extracellular Signal-Regulated MAP Kinases