title: Omega-3 fatty acids, oxidative stress, and leukocyte telomere length: A randomized controlled trial.
abstract: Shorter telomeres have been associated with poor health behaviors, age-related diseases, and early mortality. Telomere length is regulated by the enzyme telomerase, and is linked to exposure to proinflammatory cytokines and oxidative stress. In our recent randomized controlled trial, omega-3 (n-3) polyunsaturated fatty acid (PUFA) supplementation lowered the concentration of serum proinflammatory cytokines. This study assessed whether n-3 PUFA supplementation also affected leukocyte telomere length, telomerase, and oxidative stress. In addition to testing for group differences, changes in the continuous n-6:n-3 PUFA ratio were assessed to account for individual differences in adherence, absorption, and metabolism. The double-blind four-month trial included 106 healthy sedentary overweight middle-aged and older adults who received (1) 2.5g/day n-3 PUFAs, (2) l.25g/day n-3 PUFAs, or (3) placebo capsules that mirrored the proportions of fatty acids in the typical American diet. Supplementation significantly lowered oxidative stress as measured by F2-isoprostanes (p=0.02). The estimated geometric mean log-F2-isoprostanes values were 15% lower in the two supplemented groups compared to placebo. Although group differences for telomerase and telomere length were nonsignificant, changes in the n-6:n-3 PUFA plasma ratios helped clarify the intervention's impact: telomere length increased with decreasing n-6:n-3 ratios, p=0.02. The data suggest that lower n-6:n-3 PUFA ratios can impact cell aging. The triad of inflammation, oxidative stress, and immune cell aging represents important pre-disease mechanisms that may be ameliorated through nutritional interventions. This translational research broadens our understanding of the potential impact of the n-6:n-3 PUFA balance. ClinicalTrials.gov identifier: NCT00385723.
authors.affiliation: Institute for Behavioral Medicine Research, The Ohio State University College of Medicine, Columbus, OH 43210, USA. Janice.Kiecolt-Glaser@osumc.edu
authors.forename: Janice K
authors.initials: JK
authors.lastname: Kiecolt-Glaser
authors.forename: Elissa S
authors.initials: ES
authors.lastname: Epel
authors.forename: Martha A
authors.initials: MA
authors.lastname: Belury
authors.forename: Rebecca
authors.initials: R
authors.lastname: Andridge
authors.forename: Jue
authors.initials: J
authors.lastname: Lin
authors.forename: Ronald
authors.initials: R
authors.lastname: Glaser
authors.forename: William B
authors.initials: WB
authors.lastname: Malarkey
authors.forename: Beom Seuk
authors.initials: BS
authors.lastname: Hwang
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Blackburn
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016922:Cellular Senescence / Q000187:drug effects; D003863:Depression / Q000503:physiopathology; D019587:Dietary Supplements; D004311:Double-Blind Method; D015525:Fatty Acids, Omega-3 / Q000097:blood / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D019098:Telomerase / Q000187:drug effects / Q000378:metabolism; D059506:Telomere Shortening / Q000187:drug effects*
chemical_list: D015525:Fatty Acids, Omega-3; D019098:Telomerase