title: Evaluation of anti-cancer activity of Acanthester planci extracts obtained by different methods of extraction.
abstract: Acanthaster planci, the crown-of-thorns starfish, naturally endowed with the numerous toxic spines around the dorsal area of its body. Scientific investigations demonstrated several toxico-pharmacological efficacies of A. planci such as, myonecrotic activity, hemorrhagic activity, hemolytic activity, mouse lethality, phospholipase A2 (PLA2) activity, capillary permeability-increasing activity, edema-forming activity, anticoagulant activity and histamine-releasing activity from mast cells. The present study was performed to evaluate the cytotoxic activity of A. planci extracts obtained by different methods of extraction on MCF-7 and HCT-116, human breast and colon cancer cell lines, respectively. Results of the cell proliferation assay showed that PBS extract exhibited very potent cytotoxic activity against both MCF-7 and HCT-116 cell lines with IC(50) of 13.48 μg/mL and 28.78 μg/mL, respectively, while the extracts prepared by Bligh and Dyer method showed moderate cytotoxicity effect against MCF-7 and HCT-116 cell lines, for chloroform extract, IC(50) = 121.37 μg/mL (MCF-7) and 77.65 μg/mL (HCT-116), and for methanol extract, IC(50) = 46.11 μg/mL (MCF-7) and 59.29 μg/mL (HCT-116). However, the extracts prepared by sequential extraction procedure from dried starfish found to be ineffective. This study paves the way for further investigation on the peptide composition in the PBS extract of the starfish to discover potential chemotherapeutic agents.
authors.affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia Main Campus, Penang, Malaysia.
authors.forename: Ahmed Faisal
authors.initials: AF
authors.lastname: Mutee
authors.forename: Salizawati Muhamad
authors.initials: SM
authors.lastname: Salhimi
authors.forename: Farid Che
authors.initials: FC
authors.lastname: Ghazali
authors.forename: Abdalrahim Fa
authors.initials: AF
authors.lastname: Aisha
authors.forename: Chung Pin
authors.initials: CP
authors.lastname: Lim
authors.forename: Kamarruddin
authors.initials: K
authors.lastname: Ibrahim
authors.forename: Mohd Zaini
authors.initials: MZ
authors.lastname: Asmawi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation / Q000379:methods; D002725:Chloroform / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005260:Female; D045325:HCT116 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D000432:Methanol / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013215:Starfish / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D012997:Solvents; D002725:Chloroform; D000432:Methanol