title: Modulation of translation and induction of autophagy by bacterial exoproducts.
abstract: Autophagy is a catabolic process of paramount importance for cellular homeostasis during starvation. Generally, autophagy and translation are inversely regulated. Many kinds of stress lead to attenuation of translation via phosphorylation of eukaryotic translation initiation factor alpha (eIF2α). This response is conserved from yeast to man and can be either protective or detrimental depending on strength and duration of stress, and additional factors. During starvation or viral infection, phosphorylation of eIF2α is required for induction of autophagy. As exemplified here by α-hemolysin, a small pore-forming toxin (PFT) of Staphylococcus aureus and (S)-3-oxo-C12-homoserine lactone [(S)-3-oxo-C12-HSL], a quorum-sensing hormone of Pseudomonas aeruginosa, bacterial exoproducts may also impact translation and autophagy. Thereby, PFT and (S)-3-oxo-C12-HSL act differentially. Damage of the plasma membrane by PFT causes efflux of potassium, which leads to amino acid starvation and energy loss. This triggers amino acid-sensitive eIF2α-kinase GCN2, as well as energy sensor AMPK, and deactivates mTORC1. The output of this response, that is, transient metabolic reprogramming is an essential part of a defense program which enables cells to survive attack by a pore-forming agent. Thus, nutrient/energy sensors serve as sentinels of plasma membrane integrity. In contrast to PFT, (S)-3-oxo-C12-HSL does not cause acute loss of ATP or activation of GCN2, but also triggers phosphorylation of eIF2α and inhibits translation. This response appears not to depend on efflux of potassium and requires eIF2α-kinase PKR. Like α-toxin, (S)-3-oxo-C12-HSL increases lipidation of LC3 and accumulation of autophagosomes in cells. Apart from directly affecting host-cell viability, bacterial exoproducts might galvanize bystander cells to prepare for close combat with microbial offenders or inadvertently accommodate some of them.
authors.affiliation: Institute of Medical Microbiology and Hygiene, University Medical Center, Johannes Gutenberg-University Mainz, Hochhaus am Augustusplatz, 55131 Mainz, Germany.
authors.forename: Gisela
authors.initials: G
authors.lastname: von Hoven
authors.forename: Nicole
authors.initials: N
authors.lastname: Kloft
authors.forename: Claudia
authors.initials: C
authors.lastname: Neukirch
authors.forename: Sabrina
authors.initials: S
authors.lastname: Ebinger
authors.forename: Wiesia
authors.initials: W
authors.lastname: Bobkiewicz
authors.forename: Silvia
authors.initials: S
authors.lastname: Weis
authors.forename: Klaus
authors.initials: K
authors.lastname: Boller
authors.forename: Kim D
authors.initials: KD
authors.lastname: Janda
authors.forename: Matthias
authors.initials: M
authors.lastname: Husmann
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D001343:Autophagy; D001427:Bacterial Toxins / Q000378:metabolism; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000378:metabolism; D006714:Homoserine / Q000031:analogs & derivatives / Q000378:metabolism; D054884:Host-Pathogen Interactions; D006801:Humans; D010766:Phosphorylation; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity*; D013211:Staphylococcus aureus / Q000472:pathogenicity*
chemical_list: D001427:Bacterial Toxins; D015852:Eukaryotic Initiation Factor-2; D006460:Hemolysin Proteins; C109860:N-(3-oxododecanoyl)homoserine lactone; C021091:staphylococcal alpha-toxin; D006714:Homoserine; D015107:4-Butyrolactone