title: [The research on the induction of secretome by adipose tissue for adipose-derived stem cells].
abstract: OBJECTIVE
  Study the role for adipose tissue-derived stem cells (ADSCs) of secretome by adipose tissue and hoped that makes some contributions for the fat regeneration.
  
  
  METHODS
  Direct adherent culture methods and the flow cytometry (FCM) were used to isolate and identify ADSCs. Collected the medium that had contained secreted proteins as the conditional medium (CM) and was used to induce ADSCs, chemical medium group as the positive control and normal medium group as the negative control. The formation of lipid drops was observed through the cell morphology, and the reliability was verified through Real-time PCR of the mRNA level in vitro. To test the effect of CM on ADSCs in vivo, the absorbable gelatin sponge was used as a scaffold for ADSCs.
  
  
  RESULTS
  FCM ensured that the cultured cells were ADSCs. Significant increased lipid drops and related mRNA levels were observed in induced ADSCs cultured for 4 days, which indicated that the CM could promote the ADSCs differentiate into adipocytes after cultured for 4 days in vitro. The secreted proteins could promote ADSCS differentiate into vascularization tissues in vivo.
  
  
  CONCLUSIONS
  The secreted proteome may contain some growth factors that can promote ADSCs to differentiate into adipocytes and vascularization, which may give new directions for the growth factors that could be applied in the repair of soft tissue.
authors.affiliation: College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Ju-Rong
authors.initials: JR
authors.lastname: Liao
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Guo
authors.forename: Mei
authors.initials: M
authors.lastname: Yu
authors.forename: Cong
authors.initials: C
authors.lastname: Guo
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Tian
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D018919:Neovascularization, Physiologic / Q000187:drug effects; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology*
chemical_list: D020543:Proteome