title: CYP1A1 gene polymorphisms: modulator of genetic damage in coal-tar workers.
abstract: OBJECTIVE
  It is well known that polycyclic aromatic hydrocarbons (PAHs) such as benzo (a) pyrene have carcinogenic properties and may cause many types of cancers in human populations. Genetic susceptibility might be due to variation in genes encoding for carcinogen metabolizing enzymes, such as cytochrome P-450 (CYP450). Our study aimed to investigate the effect of genetic polymorphisms of CYP1A1 (m1 and m2) on genetic damage in 115 coal-tar workers exposed to PAHs in their work place.
  
  
  METHODS
  Genetic polymorphisms of CYP1A1 were determined by the PCR-RFLP method. Comet and buccal micronucleus assays were used to evaluate genetic damage among 115 coal tar workers and 105 control subjects.
  
  
  RESULTS
  Both CYP1A1 m1 and CYP1A1 m2 heterozygous and homozygous (wt/mt+mt/mt) variants individually as well as synergistically showed significant association (P<0.05) with genetic damage as measured by tail moment (TM) and buccal micronuclei (BMN) frequencies in control and exposed subjects.
  
  
  CONCLUSIONS
  In our study we found significant association of CYP1A1 m1 and m2 heterozygous (wt/mt) +homozygous (mt/mt) variants with genetic damage suggesting that these polymorphisms may modulate the effects of PAH exposure in occupational settings.
authors.affiliation: Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India.
authors.forename: Shiv Kumar
authors.initials: SK
authors.lastname: Giri
authors.forename: Anita
authors.initials: A
authors.lastname: Yadav
authors.forename: Anil
authors.initials: A
authors.lastname: Kumar
authors.forename: Kapil
authors.initials: K
authors.lastname: Dev
authors.forename: Sachin
authors.initials: S
authors.lastname: Gulati
authors.forename: Ranjan
authors.initials: R
authors.lastname: Gupta
authors.forename: Neeraj
authors.initials: N
authors.lastname: Aggarwal
authors.forename: Sanjeev Kumar
authors.initials: SK
authors.lastname: Gautam
mesh_terms: D000328:Adult; D001564:Benzo(a)pyrene / Q000506:poisoning; D002617:Chemical Industry; D003033:Coal Tar / Q000506:poisoning*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D004249:DNA Damage; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D015162:Micronucleus Tests / Q000379:methods; D009369:Neoplasms / Q000139:chemically induced* / Q000201:enzymology / Q000235:genetics*; D009784:Occupational Diseases / Q000139:chemically induced* / Q000201:enzymology / Q000235:genetics*; D016273:Occupational Exposure / Q000009:adverse effects*; D011084:Polycyclic Aromatic Hydrocarbons / Q000506:poisoning; D011110:Polymorphism, Genetic; D017132:Workplace
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D001564:Benzo(a)pyrene; D003033:Coal Tar; C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1