title: Radio-protective effect of catalpol in cultured cells and mice.
abstract: Ionizing radiation can induce DNA damage and cell death by generating reactive oxygen species (ROS). The objective of this study was to investigate the radio-protective effect of catalpol (a main bioactive component in the traditional Chinese Rehmannia) on irradiated cells and mice. We found that treating cells with catalpol (25-100 μg/ml) before irradiation could significantly inhibit ionizing radiation (IR)-induced human lymphocyte AHH-1 cells apoptosis and increase cells viability in vitro. At the same time our study also showed that catalpol (25-100 mg/kg) reduced morphological damage of the gastrointestinal tract by 15.6%, 33.3% and 44.4%, respectively compared with the radiation-induced group, decreased plasma malondialdehyde (MDA) intestinal 8-hydroxydeoxyguanosine (8-OHdG) levels and increased plasma endogenous antioxidants and peripheral white blood cells and platelets in vivo. These results suggest that catalpol possesses notable radio-protective activity, which might be related to its effect of reducing ROS.
authors.affiliation: Department of Traditional Chinese Medicine, Changhai Hospital, 174 Changhai Road, Shanghai 200433, PR China.
authors.forename: Chengjie
authors.initials: C
authors.lastname: Chen
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chen
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.forename: Chao
authors.initials: C
authors.lastname: Zhu
authors.forename: Fanfu
authors.initials: F
authors.lastname: Fang
authors.forename: Shi
authors.initials: S
authors.lastname: Shu
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Changquan
authors.initials: C
authors.lastname: Ling
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D007410:Intestinal Diseases / Q000473:pathology / Q000517:prevention & control*; D057888:Iridoid Glucosides / Q000008:administration & dosage*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011832:Radiation Injuries / Q000473:pathology / Q000517:prevention & control*; D011836:Radiation Tolerance / Q000187:drug effects / Q000502:physiology*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D016896:Treatment Outcome
chemical_list: D057888:Iridoid Glucosides; D011837:Radiation-Protective Agents; C078040:catalpol