title: Evaluation of the cobas 4800 CT/NG Test for detecting Chlamydia trachomatis and Neisseria gonorrhoeae DNA in urogenital swabs and urine specimens.
abstract: We have evaluated 696 samples (488 swabs and 208 urine specimens) with the cobas 4800 (c4800) CT/NG Test for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae DNA in swab and urine specimens. c4800 results were compared with those obtained from COBAS AMPLICOR (CAM) CT/NG Test. Discordant results were reanalyzed with the MultiNA system and compared with clinical data. For C. trachomatis detection by both methods, we obtained 93.8%, 100%, 100%, and 99.1% for sensitivity, specificity, and positive and negative predictive values, respectively. For urine specimens analyzed in c4800, our results were 96.6%, 100%, 100%, and 99.4%, respectively. For N. gonorrhoeae detection, swab results were:88.0%, 100%, 100%, and 99.4%. For urine specimen, results obtained were 100%, 100%, 100%, and 100%. Reanalyses were all concordant between both methods. c4800 results were comparable with those obtained with the CAM system. We had an excellent correlation between swab and urine specimens analyzed by c4800.
authors.affiliation: Unidad de Enfermedades Infecciosas y Microbiología Clínica, Instituto de Biomedicina de Sevilla, University Hospital of Valme. Av. Bellavista, 41014 Seville, Spain. manuel.parra.exts@juntadeandalucia.es
authors.forename: Manuel
authors.initials: M
authors.lastname: Parra-Sánchez
authors.forename: Jose Carlos
authors.initials: JC
authors.lastname: Palomares
authors.forename: Samuel
authors.initials: S
authors.lastname: Bernal
authors.forename: María Trinidad
authors.initials: MT
authors.lastname: González
authors.forename: Nieves
authors.initials: N
authors.lastname: Sivianes
authors.forename: Luis
authors.initials: L
authors.lastname: Pérez
authors.forename: Isabel
authors.initials: I
authors.lastname: Pueyo
authors.forename: Estrella
authors.initials: E
authors.lastname: Martín-Mazuelos
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005835:Genitalia / Q000382:microbiology*; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D015231:Sexually Transmitted Diseases, Bacterial / Q000175:diagnosis* / Q000382:microbiology; D014556:Urine / Q000382:microbiology*