title: [Study of the effect of temperature on the conformation of ovotransferrin by two-dimensional infrared correlation spectroscopy].
abstract: The conformation changes of Apo-Ovotransferrin and Holo-Ovotransferrin were studied with the heat treatment 25-95 degrees C by using Fourier transform infrared spectroscopy (FTIR) and two-dimensional correlation spectroscopy analyzer. The results of one-dimensional infrared spectroscopy showed that with the increase in temperature, the peak at 3 300 cm(-1) of Apo-Ovo-transferrin shifted more than that of Holo-Ovotransferrin. The peak at 3 300 cm(-1) derived from stretching vibrations of N-H and O-H indicates that iron-binding enhanced the role of hydrogen bonds and resistance to heat. The changing order of the secondary structure of ovotransferrin was determined by analyzing two-dimensional infrared spectra,witch is beta-sheet>amide II >-CH2 - bending vibration. In addition, it was found that the cross-peaks at 1 652 and 1 688 cm(-1) are different in synchronous and asynchronous counter maps by comparing Apo-Ovotransferrin with Holo-Ovotransferrin. It was suggested that the temperature made less impact on the alpha-helix in Holo-Ovotransferrin than on that in Apo-Ovotransferrin, however, the beta-turn in Holo-Ovotransferrin was more sensitive to temperature.
authors.affiliation: National Research and Development Center for Egg Processing, Huazhong Agricultural University, Wuhan 430070, China. renet312@163.com
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Ren
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Guo
authors.forename: Fang
authors.initials: F
authors.lastname: Geng
authors.forename: Mei-Hu
authors.initials: MH
authors.lastname: Ma
authors.forename: Qun
authors.initials: Q
authors.lastname: Huang
authors.forename: Xiao-Fen
authors.initials: XF
authors.lastname: Wu
mesh_terms: D000577:Amides; D003207:Conalbumin / Q000737:chemistry*; D006860:Hydrogen Bonding; D017433:Protein Structure, Secondary; D013055:Spectrophotometry, Infrared; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D000577:Amides; D003207:Conalbumin