title: [CHROMagar KPC. Comparison with the method proposed by the Centers for Disease Control and Prevention (CDC, USA) for rectal screening and evaluation of false positive results].
abstract: Eighty one rectal swabs (RS) were cultured on CHROMagar KPC and the CDC method. Of the 81 samples, 9 were positive for KPC-producing Klebsiella pneumoniae on CHROMagar KPC, and 6 for the CDC method. CHROMagar KPC had two false positive (FP) results: 1 K. pneumoniae and 1 Acinetobacter sp. FP results on the CDC method were: 25 Acinetobacter spp., 2 Escherichia coli and 4 K. pneumoniae: CHROMagar KPC yielded a better recovery of KPC-producing bacteria and less FP results than CDC method. In order to evaluate FP results on CHROMagar KPC, 1247 RS were cultured and yielded 1021 negatives, 171 KPC-producing K. pneumoniae and 55 FP (4.4 %). Because of the FP results growing on CHROMagar KPC, KPC must be phenotypically confirmed in the bacteria isolated.
authors.affiliation: Hospital Juan A Fernández Cerviño 3356 (1425) Ciudad Autónoma de Buenos Aires, Argentina. lerrecalde@yahoo.com.ar
authors.forename: Laura
authors.initials: L
authors.lastname: Errecalde
authors.forename: Sandra
authors.initials: S
authors.lastname: Cogut
authors.forename: Mariana
authors.initials: M
authors.lastname: Erbín
authors.forename: Liliana
authors.initials: L
authors.lastname: Jordá Vargas
authors.forename: Eugenia
authors.initials: E
authors.lastname: Cattani
authors.forename: Tamara
authors.initials: T
authors.lastname: Posse
authors.forename: Silvia
authors.initials: S
authors.lastname: Tutzer
authors.forename: Ricardo
authors.initials: R
authors.lastname: Hermes
authors.forename: Sara
authors.initials: S
authors.lastname: Kaufman
mesh_terms: D000150:Acinetobacter / Q000201:enzymology; D000362:Agar; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D015780:Carbapenems / Q000494:pharmacology*; D002487:Centers for Disease Control and Prevention, U.S.; D002863:Chromogenic Compounds; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology; D005189:False Positive Reactions; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D008403:Mass Screening; D010641:Phenotype; D012007:Rectum / Q000382:microbiology*; D014481:United States; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000032:analysis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D002863:Chromogenic Compounds; D003470:Culture Media; D000362:Agar; D001618:beta-Lactamases; C531581:carbapenemase-2, Klebsiella pneumoniae