title: Method for comparative analysis of ribonucleic acids using isotope labeling and mass spectrometry.
abstract: Here, we describe a method for the comparative analysis of ribonucleic acids (RNAs). This method allows sequence or modification information from a previously uncharacterized RNA to be obtained by direct comparison with a reference RNA, whose sequence or modification information is known. This simple and rapid method is enabled by the differential labeling of two RNA samples. One sample, the reference RNA, is labeled with (16)O during enzymatic digestion. The second sample, the candidate or unknown RNA, is labeled with (18)O. By combining the two digests, digestion products that share the same sequence or post-transcriptional modification(s) between the reference and candidate will appear as doublets separated by 2 Da. Sequence or modification differences between the two will generate singlets that can be further characterized to identify how the candidate sequence differs from the reference. We illustrate the application of this approach for sequencing individual RNAs and demonstrate how this method can be used to identify sequence-specific differences in RNA modification. This comparative analysis of RNA digests (CARD) approach is scalable to multiple candidate RNAs using one or multiple reference RNAs and is compatible with existing methods for quantitative analysis of RNAs.
authors.affiliation: Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, PO Box 210172, University of Cincinnati, Cincinnati, Ohio 45221-0172, United States.
authors.forename: Siwei
authors.initials: S
authors.lastname: Li
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Limbach
mesh_terms: D001483:Base Sequence; D041041:Citrobacter koseri / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010103:Oxygen Isotopes / Q000032:analysis; D012329:RNA, Bacterial / Q000032:analysis* / Q000235:genetics; D012343:RNA, Transfer / Q000032:analysis / Q000235:genetics; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D010103:Oxygen Isotopes; D012329:RNA, Bacterial; D012343:RNA, Transfer