title: Toxin detection in patients' sera by mass spectrometry during two outbreaks of type A Botulism in France.
abstract: In two outbreaks of food-borne botulism in France, Clostridium botulinum type A was isolated and characterized from incriminated foods. Botulinum neurotoxin type A was detected in the patients' sera by mouse bioassay and in vitro endopeptidase assay with an immunocapture step and identification of the cleavage products by mass spectrometry.
authors.affiliation: Institut Pasteur, Unité des Bactéries Anaérobies et Toxines, Paris, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Mazuet
authors.forename: Eric
authors.initials: E
authors.lastname: Ezan
authors.forename: Hervé
authors.initials: H
authors.lastname: Volland
authors.forename: Michel Robert
authors.initials: MR
authors.lastname: Popoff
authors.forename: François
authors.initials: F
authors.lastname: Becher
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods; D019274:Botulinum Toxins, Type A / Q000097:blood*; D001906:Botulism / Q000175:diagnosis* / Q000453:epidemiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D004196:Disease Outbreaks; D005517:Foodborne Diseases / Q000175:diagnosis / Q000453:epidemiology; D005602:France / Q000453:epidemiology; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D044967:Serum / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D019274:Botulinum Toxins, Type A