title: [Two multidrug-resistant Salmonella infantis isolates behave like hypo-invasive strains but have high intracellular proliferation].
abstract: In this work, plasmid-encoded virulence factors in two Salmonella Infantis isolates carrying multiresistance plasmids were investigated. In addition, their invasion and proliferative ability in non-phagocytic cells was studied. None of them showed the typical determinants of virulence plasmids (spy operon). The invasion assays of S. Infantis isolates on eukaryotic cells showed a decreased ability to invade but they remained and proliferated in the cytoplasm regardless of having used a permissive (HeLa) or non-permissive (NRK) cell line. Finally, there was no microscopic evidence suggesting a bactericidal effect of these eukaryotic cell lines on the isolates tested.
authors.affiliation: Facultad de Farmacia y Bioquímica - Universidad de BuenosAires, Junin 956 (1113) Ciudad Autónoma de Buenos Aires, Argentina. jdiconza@fbcb.unl.edu.ar
authors.forename: José A
authors.initials: JA
authors.lastname: Di Conza
authors.forename: Marta E
authors.initials: ME
authors.lastname: Mollerach
authors.forename: Gabriel O
authors.initials: GO
authors.lastname: Gutkind
authors.forename: Juan A
authors.initials: JA
authors.lastname: Ayala
mesh_terms: D000818:Animals; D001769:Blood / Q000382:microbiology; D002455:Cell Division; D002460:Cell Line / Q000382:microbiology; D003428:Cross Infection / Q000382:microbiology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005057:Eukaryotic Cells / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D005819:Genetic Markers; D006367:HeLa Cells / Q000382:microbiology; D006801:Humans; D007668:Kidney / Q000166:cytology; D011815:R Factors / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D051381:Rats; D012475:Salmonella / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D012480:Salmonella Infections / Q000382:microbiology; D014774:Virulence / Q000235:genetics
chemical_list: D005819:Genetic Markers