title: A multi-technique approach for the quantification of Microcystis aeruginosa FACHB-905 biomass during high algae-laden periods.
abstract: A pronounced dominance of toxic cyanobacteria has been found in eutrophic water bodies, with Microcystis being a common species. Although toxic cyanobacteria are commonly described worldwide, few recent papers on the sensitive and effective quantification of cyanobacteria have been published. In this paper, a multi-technique approach was applied by the use of cell density counting, cell viability testing, chlorophyll a determination, microcystin monitoring and gene extraction techniques to quantitatively analyse the cyanobacterial biomass of Microcystis aeruginosa FACHB-905. The entire dataset was used to examine the relationships between these indices. Results showed that, for 10(7) viable cells in the experimental conditions, the contents of chlorophyll a, microcystin-LR and total genes (16S rDNA) averaged 2.65 microg, 0.61 microg and 0.79 microg, respectively. For different cell viability proportions in the same particular phase of growth, it is easy to obtain the respective amount of viable cells and inactive cells and their measurable indices when any one of the three indices, chlorophyll a, DNA or microcystin-LR, is measured. This study provides a new perspective and method for determining multiple indices of toxic cyanobacteria during the same conditions and phases.
authors.affiliation: School of Environmental Science and Engineering, Shandong University, Jinan, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Sun
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Pei
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Hu
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Song
mesh_terms: D018533:Biomass; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal; D004784:Environmental Monitoring / Q000379:methods*; D008387:Marine Toxins; D052998:Microcystins / Q000032:analysis / Q000378:metabolism; D046931:Microcystis / Q000166:cytology* / Q000235:genetics / Q000254:growth & development*; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D008387:Marine Toxins; D052998:Microcystins; D002734:Chlorophyll; C057862:cyanoginosin LR; D000077194:Chlorophyll A