title: Response of hepatic lipid and glucose metabolism to a mixture or single fatty acids: Possible presence of fatty acid-sensing mechanisms.
abstract: To assess the hypothesis that an acute dietary fatty acid (FA) supply may improve glucose tolerance in rainbow trout, we orally administered fish with fish oil (FO; 10mL.kg(-1), one time), which were then subjected to a glucose tolerance test and sampled 6h after injection. Parameters related to glucose and lipid metabolism were then assessed. The results suggest that when both nutrients were administered at the same time, an increased potential for lipogenesis occurred concomitantly with a lower level of glycaemia. In a second experiment we administered intraperitoneally a single FA present in the FO mixture such as oleic acid (60 or 300μg.kg(-1)) whereas octanoic acid (60 or 300μg.kg(-1)) was used as negative control (absent from the FO). However, the effects of both FA were similar in reducing the potential of lipid synthesis and oxidation, and in enhancing the potential of glucose synthesis and glycogenesis. Differences found between FO and single FA administration show that response to FA was dependent on the treatment (mixture vs. single FA) but also comply with the idea that an interaction between FA and glucose rather than FA alone are in the origin of the results reported. The administration of individual FA such as oleic and octanoic acid failed in enhancing lipogenesis and reducing plasma glucose levels and thus in explaining results obtained with FO. However, results provide evidence that FA even provided at a low dose play a key role in the regulation of several putative components of a FA sensing system present in rainbow trout liver.
authors.affiliation: Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, E-36310 Vigo, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Librán-Pérez
authors.forename: A Cláudia
authors.initials: AC
authors.lastname: Figueiredo-Silva
authors.forename: Stephane
authors.initials: S
authors.lastname: Panserat
authors.forename: Inge
authors.initials: I
authors.lastname: Geurden
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Míguez
authors.forename: Sergio
authors.initials: S
authors.lastname: Polakof
authors.forename: José L
authors.initials: JL
authors.lastname: Soengas
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D002210:Caprylates / Q000494:pharmacology; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D004359:Drug Therapy, Combination / Q000379:methods; D004789:Enzyme Activation; D064429:Fatty Acid Synthases / Q000378:metabolism; D005227:Fatty Acids / Q000494:pharmacology; D005395:Fish Oils / Q000494:pharmacology; D029941:Fish Proteins / Q000032:analysis / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D005951:Glucose Tolerance Test / Q000379:methods; D005952:Glucose-6-Phosphatase / Q000235:genetics / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism; D050155:Lipogenesis; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D019301:Oleic Acid / Q000494:pharmacology; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D001786:Blood Glucose; D002210:Caprylates; D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D029941:Fish Proteins; D012333:RNA, Messenger; D019301:Oleic Acid; D005954:Glucosephosphate Dehydrogenase; D064429:Fatty Acid Synthases; D001275:ATP Citrate (pro-S)-Lyase; D005952:Glucose-6-Phosphatase; D005947:Glucose; C031492:octanoic acid