title: Genetic relationships among five varieties of Curcuma  alismatifolia (Zingiberaceae) based on ISSR markers.
abstract: The genus Curcuma is a member of the ginger  family (Zingiberaceae) that has recently become popular for use as flowering  pot plants, both indoors and as patio and landscape plants. We used PCR-based  molecular markers (ISSRs) to assess genetic variation and relationships between  five varieties of curcuma (Curcuma alismatifolia) cultivated in  Malaysia. Sixteen ISSR primers generated 139 amplified fragments, of which 77%  had high polymorphism among these varieties. These markers were used to  estimate genetic similarity among the varieties using Jaccard's similarity  coefficient. The similarity matrix was used to construct a dendrogram, and a  principal component plot was developed to examine genetic relationships among  varieties. Similarity coefficient values ranged from 0.40 to 0.58 (with a mean  of 0.5) among the five varieties. The mean value of number of observed alleles,  number of effective alleles, mean Nei's gene diversity, and Shannon's  information index were 8.69, 1.48, 0.29, and 0.43, respectively.
authors.affiliation: Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, Selangor, Malaysia. sima_taheri65@yahoo.com
authors.forename: S
authors.initials: S
authors.lastname: Taheri
authors.forename: T L
authors.initials: TL
authors.lastname: Abdullah
authors.forename: N A P
authors.initials: NA
authors.lastname: Abdullah
authors.forename: Z
authors.initials: Z
authors.lastname: Ahmad
mesh_terms: D030024:Curcuma / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D025341:Principal Component Analysis
chemical_list: D005819:Genetic Markers