title: Production of Plasmodium falciparum gametocytes in vitro.
abstract: Production of gametocytes in vitro is essential for studies of Plasmodium falciparum sexual stages. Here, we describe procedures for the high-yield production and fractionation of P. falciparum gametocytes stages I to V.
authors.affiliation: Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Public Health, Baltimore, MD, USA.
authors.forename: Kathryn Shaw
authors.initials: KS
authors.lastname: Saliba
authors.forename: Marcelo
authors.initials: M
authors.lastname: Jacobs-Lorena
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D004912:Erythrocytes / Q000469:parasitology / Q000473:pathology; D005854:Germ Cells / Q000254:growth & development*; D006801:Humans; D008018:Life Cycle Stages; D010963:Plasmodium falciparum / Q000302:isolation & purification / Q000502:physiology*