title: Distinct subcellular localization of tRNA-derived fragments in the infective metacyclic forms of Trypanosoma cruzi.
abstract: Small non-coding RNAs derived from transfer RNAs have been identified as a broadly conserved prokaryotic and eukaryotic response to stress. Their presence coincides with changes in developmental state associated with gene expression regulation. In the epimastigote form of Trypanosoma cruzi, tRNA fragments localize to posterior cytoplasmic granules. In the infective metacyclic form of the parasite, we found tRNA-derived fragments to be abundant and evenly distributed within the cytoplasm. The fragments were not associated with polysomes, suggesting that the tRNA-derived fragments may not be directly involved in translation control in metacyclics.
authors.affiliation: Instituto Carlos Chagas, Fiocruz, Curitiba, PR, Brasil.
authors.forename: Larissa
authors.initials: L
authors.lastname: Reifur
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Garcia-Silva
authors.forename: Saloê Bispo
authors.initials: SB
authors.lastname: Poubel
authors.forename: Lysangela Ronalte
authors.initials: LR
authors.lastname: Alves
authors.forename: Paulo
authors.initials: P
authors.lastname: Arauco
authors.forename: Diane Kelly
authors.initials: DK
authors.lastname: Buiar
authors.forename: Samuel
authors.initials: S
authors.lastname: Goldenberg
authors.forename: Alfonso
authors.initials: A
authors.lastname: Cayota
authors.forename: Bruno
authors.initials: B
authors.lastname: Dallagiovanna
mesh_terms: D003594:Cytoplasmic Granules / Q000737:chemistry / Q000235:genetics*; D016053:RNA, Protozoan / Q000032:analysis* / Q000235:genetics; D012343:RNA, Transfer / Q000032:analysis* / Q000235:genetics; D014349:Trypanosoma cruzi / Q000235:genetics*
chemical_list: D016053:RNA, Protozoan; D012343:RNA, Transfer