title: [Investigation of the anti-terminal maturation effects of parathyroid hormone on human osteoarthritis chondrocytes].
abstract: OBJECTIVE
  To investigate the inhibition in parathyroid hormone (PTH) to terminal differentiation of human osteoarthritis chondrocytes.
  
  
  METHODS
  Human osteoarthritis chondrocytes were isolated and cultured. The cells were divided into PTH group and control group, and the cells of two groups were treated respectively with PTH and saline every day. The morphology of cells was observed by microscope. Alkaline phosphatase (ALP) staining was performed to detect the ALP protein. The hypertrophic marker genes mRNA and protein expressions were detected by real-time RT-PCR and Westernblot.
  
  
  RESULTS
  Human osteoarthritis chondrocytes had characteristic of terminal differentiation. Compared with control group, PTH group can obviously degrade ALP staining density, and decrease marker gene mRNA and proteinic expression of promoting terminal differentiation.
  
  
  CONCLUSIONS
  PTH has a role of inhibition of terminal differentiation of human osteoarthritis chondrocytes.
authors.affiliation: Department of Spinal and Joint Surgery, the Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, China. tianyecmu2h@sina.com
authors.forename: Tian
authors.initials: T
authors.lastname: Ye
authors.forename: Xu
authors.initials: X
authors.lastname: Ying
authors.forename: Fu
authors.initials: F
authors.lastname: Qin
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D010003:Osteoarthritis / Q000188:drug therapy* / Q000473:pathology; D010281:Parathyroid Hormone / Q000494:pharmacology*
chemical_list: D010281:Parathyroid Hormone; D000469:Alkaline Phosphatase