title: Effect of 300 mT static and 50 Hz 0.1 mT extremely low frequency magnetic fields on  Tuber borchii  mycelium.
abstract: The present work aimed to investigate whether exposure to static magnetic field (SMF) and extremely low frequency magnetic field (ELF-MF) can induce biomolecular changes on  Tuber borchii  hyphal growth. Tuber borchii mycelium was exposed for 1 h for 3 consecutive days to a SMF of 300 mT or an ELF-MF of 0.1 mT 50 Hz. Gene expression and biochemical analyses were performed. In mycelia exposed to ELF-MF, some genes involved in hyphal growth, investigated using quantitative real-time polymerase chain reaction, were upregulated, and the activity of many glycolytic enzymes was increased. On the contrary, no differences were observed in gene expression after exposure to SMF treatment, and only the activities of glucose 6-phosphate dehydrogenase and hexokinase increased. The data herein presented suggest that the electromagnetic field can act as an environmental factor in promoting hyphal growth and can be used for applicative purposes, such as the set up of new in vitro cultivation techniques.
authors.affiliation: Department of Biomolecular Science, Universiy of Urbino Carlo Bo, Via A. Saffi 2, 61029 Urbino, Italy. lucia.potenza@uniurb.it
authors.forename: Lucia
authors.initials: L
authors.lastname: Potenza
authors.forename: Roberta
authors.initials: R
authors.lastname: Saltarelli
authors.forename: Emanuela
authors.initials: E
authors.lastname: Polidori
authors.forename: Paola
authors.initials: P
authors.lastname: Ceccaroli
authors.forename: Antonella
authors.initials: A
authors.lastname: Amicucci
authors.forename: Sabrina
authors.initials: S
authors.lastname: Zeppa
authors.forename: Alessandra
authors.initials: A
authors.lastname: Zambonelli
authors.forename: Vilberto
authors.initials: V
authors.lastname: Stocchi
mesh_terms: D004574:Electromagnetic Fields; D004734:Energy Metabolism / Q000528:radiation effects; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects; D005800:Genes, Fungal / Q000235:genetics; D005947:Glucose / Q000378:metabolism; D025301:Hyphae / Q000254:growth & development / Q000528:radiation effects; D060526:Magnetic Fields; D025282:Mycelium / Q000235:genetics / Q000528:radiation effects*; D004718:Saccharomycetales / Q000235:genetics / Q000528:radiation effects*
chemical_list: D005947:Glucose