title: The nuclear envelope proteome differs notably between tissues.
abstract: One hypothesis to explain how mutations in the same nuclear envelope proteins yield pathologies focused in distinct tissues is that as yet unidentified tissue-specific partners mediate the disease pathologies. The nuclear envelope proteome was recently determined from leukocytes and muscle. Here the same methodology is applied to liver and a direct comparison of the liver, muscle and leukocyte data sets is presented. At least 74 novel transmembrane proteins identified in these studies have been directly confirmed at the nuclear envelope. Within this set, RT-PCR, western blot and staining of tissue cryosections confirms that the protein complement of the nuclear envelope is clearly distinct from one tissue to another. Bioinformatics reveals similar divergence between tissues across the larger data sets. For proteins acting in complexes according to interactome data, the whole complex often exhibited the same tissue-specificity. Other tissue-specific nuclear envelope proteins identified were known proteins with functions in signaling and gene regulation. The high tissue specificity in the nuclear envelope likely underlies the complex disease pathologies and argues that all organelle proteomes warrant re-examination in multiple tissues.
authors.affiliation: Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.
authors.forename: Nadia
authors.initials: N
authors.lastname: Korfali
authors.forename: Gavin S
authors.initials: GS
authors.lastname: Wilkie
authors.forename: Selene K
authors.initials: SK
authors.lastname: Swanson
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Srsen
authors.forename: Jose
authors.initials: J
authors.lastname: de Las Heras
authors.forename: Dzmitry G
authors.initials: DG
authors.lastname: Batrakou
authors.forename: Poonam
authors.initials: P
authors.lastname: Malik
authors.forename: Nikolaj
authors.initials: N
authors.lastname: Zuleger
authors.forename: Alastair R W
authors.initials: AR
authors.lastname: Kerr
authors.forename: Laurence
authors.initials: L
authors.lastname: Florens
authors.forename: Eric C
authors.initials: EC
authors.lastname: Schirmer
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019295:Computational Biology; D006801:Humans; D007962:Leukocytes / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008565:Membrane Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D009685:Nuclear Envelope / Q000378:metabolism*; D009928:Organ Specificity; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D008565:Membrane Proteins; D020543:Proteome