title: Quantitative estimation of gymnemagenin in Gymnema sylvestre extract and its marketed formulations using the HPLC-ESI-MS/MS method.
abstract: BACKGROUND
  Gymnema sylvestre, with gymnemic acids as active pharmacological constituents, is a popular ayurvedic herb and has been used to treat diabetes, as a remedy for cough and as a diuretic. However, very few analytical methods are available for quality control of this herb and its marketed formulations.
  
  
  OBJECTIVE
  To develop and validate a new, rapid, sensitive and selective HPLC-ESI (electrospray ionisation)-MS/MS method for quantitative estimation of gymnemagenin in G. sylvestre and its marketed formulations.
  
  
  METHODS
  HPLC-ESI-MS/MS method using a multiple reactions monitoring mode was used for quantitation of gymnemagenin. Separation was carried out on a Luna C-18 column using gradient elution of water and methanol (with 0.1% formic acid and 0.3% ammonia).
  
  
  RESULTS
  The developed method was validated as per International Conference on Harmonisation Guideline ICH-Q2B and found to be accurate, precise and linear over a relatively wide range of concentrations (5.280-305.920 ng/mL). Gymnemagenin contents were found from 0.056 ± 0.002 to 4.77 ± 0.59% w/w in G. sylvestre and its marketed formulations.
  
  
  CONCLUSIONS
  The method established is simple, rapid, with high sample throughput, and can be used as a tool for quality control of G. sylvestre and its formulations.
authors.affiliation: Department of Pharmacognosy, J.S.S. College of Pharmacy, Rocklands, Ootacamund 643 001, Tamilnadu, India. k.bhagyashree@gmail.com
authors.forename: Bhagyashree
authors.initials: B
authors.lastname: Kamble
authors.forename: Ankur
authors.initials: A
authors.lastname: Gupta
authors.forename: Dada
authors.initials: D
authors.lastname: Patil
authors.forename: Shirish
authors.initials: S
authors.lastname: Janrao
authors.forename: Laxman
authors.initials: L
authors.lastname: Khatal
authors.forename: B
authors.initials: B
authors.lastname: Duraiswamy
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000737:chemistry / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031104:Gymnema sylvestre / Q000737:chemistry*; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D004364:Pharmaceutical Preparations; D010936:Plant Extracts; C517988:gymnemagenin