title: Genetic characterization of parvoviruses circulating in turkey and chicken flocks in Poland.
abstract: Between 2008 and 2011, commercial turkey and chicken flocks in Poland were examined for the presence of turkey parvovirus (TuPV) and chicken parvovirus (ChPV). Clinical samples (10 individual faecal swabs/flock) from 197 turkey flocks (turkeys aged 1 to 19 weeks) and 45 chicken flocks (chickens aged 3 to 17 weeks) were collected in different regions of the country and tested using a PCR assay that targeted the NS1 gene (3'ORF). The prevalence of TuPV was 29.4 % in the flocks tested, while ChPV infections were found in 22.2 % of the studied flocks. Phylogenetic analysis revealed a clear division into three groups: ChPV-like, TuPV-like and a third, previously unrecognized and distinct subgroup, TuPV-LUB, containing exclusively three Polish isolates from turkeys. The isolates from the novel group showed as little as 50.6-64.5 % of nucleotide sequence identity to the prototype chicken and turkey parvovirus strains. Genetic analysis of a ChPV isolate that was classified in the TuPV group strongly suggests a recombination event between chicken and turkey parvoviruses.
authors.affiliation: Department of Poultry Diseases, National Veterinary Research Institute, Al. Partyzantow 57, 24-100 Pulawy, Poland. domanska@piwet.pulawy.pl
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Domanska-Blicharz
authors.forename: Anna
authors.initials: A
authors.lastname: Jacukowicz
authors.forename: Anna
authors.initials: A
authors.lastname: Lisowska
authors.forename: Zenon
authors.initials: Z
authors.lastname: Minta
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D005243:Feces / Q000821:virology; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017992:Parvovirus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011044:Poland / Q000453:epidemiology; D011201:Poultry Diseases / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence; D014422:Turkeys; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C106202:NS1 protein, parvovirus; D017361:Viral Nonstructural Proteins