title: Perinatal manipulation of α-linolenic acid intake induces epigenetic changes in maternal and offspring livers.
abstract: Previous studies indicated that the intake of α-linolenic acid (ALA) can alter the concentration of both ω-6 and ω-3 fatty acids in both mother and offspring, with consequences on postnatal brain development. This study describes the association between maternal ALA availability during gestation and lactation, and alterations in the Fads2 DNA methylation in both maternal and offspring livers, at the end of lactation period. Both Fads2 promoter and intron 1 DNA methylation were increased in the groups receiving postnatal flaxseed oil containing 50% ALA (mothers or pups), while bivariate analysis indicated a significant association of the Fads2 epigenetic status in the liver between each mother and its offspring. In addition, Fads2 expression was negatively correlated with promoter methylation at the individual level in maternal livers (P<0.05). This study also indicated that the interplay between ALA availability during gestation and lactation can differentially alter the expression of desaturases and elongases involved in ω-6 and ω-3 metabolic pathways. In summary, when considering the perinatal dietary ALA requirements in mice, both gestation and lactation periods should be considered as having distinct roles in modulating the metabolism of ω-6 and ω-3 fatty acids in maternal mouse livers.
authors.affiliation: University of North Carolina Nutrition Research Institute, Kannapolis, North Carolina 28081, USA. mihai_niculescu@unc.edu
authors.forename: Mihai D
authors.initials: MD
authors.lastname: Niculescu
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Lupu
authors.forename: Corneliu N
authors.initials: CN
authors.lastname: Craciunescu
mesh_terms: D000818:Animals; D001483:Base Sequence; D019175:DNA Methylation; D017931:DNA Primers; D044127:Epigenesis, Genetic; D044943:Fatty Acid Desaturases / Q000235:genetics; D005227:Fatty Acids / Q000097:blood; D005260:Female; D007774:Lactation; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017962:alpha-Linolenic Acid / Q000008:administration & dosage*
chemical_list: D017931:DNA Primers; D005227:Fatty Acids; D012333:RNA, Messenger; D017962:alpha-Linolenic Acid; D044943:Fatty Acid Desaturases