title: [Comparison of transformation of four processed rhubarb aqueous extracts in intestinal bacteria in vitro].
abstract: OBJECTIVE
  To compare the metabolic transformation of four processed rhubarb aqueous extracts in rat intestinal bacteria in vitro.
  
  
  METHODS
  Rat intestinal bacteria test solution and each of four processed rhubarb aqueous extracts were incubated under anaerobic conditions at 37 degrees C. High-performance liquid chromatography with diode-array detection (HPLC-DAD) and tandem mass spectrometry (HPLC-MS/MS) was used for the qualitative analysis on the components that can be bio-transformed by rat intestinal bacteria as well as the trend of metabolic transformation of each parent compounds according to the changes in chromatographic peak areas in different incubation times.
  
  
  RESULTS
  Anthraquinones, glucose gallates and naphthalenes glucosides could be bio-transformed by rat intestinal bacteria. Of them, anthraquinones were undoubtedly the most prevalent parent compounds, as 12 out of the 17 metabolites were tentatively assigned as metabolites transformed from anthraquinones. Besides, it was also found that each parent compound in four processed rhubarb extract were diverse from each other with the incubation time.
  
  
  CONCLUSIONS
  The preparations change composition and proportional relationship of ingredients contained in rhubarb and thus impacting their transformation effect in intestinal bacteria.
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China. songrui@cpu.edu.cn
authors.forename: Rui
authors.initials: R
authors.lastname: Song
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tian
authors.forename: Zunjian
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001711:Biotransformation; D002626:Chemistry, Pharmaceutical; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000378:metabolism*; D007408:Intestinal Absorption; D007422:Intestines / Q000382:microbiology*; D051381:Rats; D012250:Rheum / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D014867:Water