title: Automated recovery of the center of rotation in optical projection tomography in the presence of scattering.
abstract: Finding the center of rotation is an essential step for accurate three-dimensional reconstruction in optical projection tomography (OPT). Unfortunately current methods are not convenient since they require either prior scanning of a reference phantom, small structures of high intensity existing in the specimen, or active participation during the centering procedure. To solve these problems this paper proposes a fast and automatic center of rotation search method making use of parallel programming in graphics processing units (GPUs). Our method is based on a two step search approach making use only of those sections of the image with high signal to noise ratio. We have tested this method both in non-scattering ex vivo samples and in in vivo specimens with a considerable contribution of scattering such as Drosophila melanogaster pupae, recovering in all cases the center of rotation with a precision 1/4 pixel or less.
authors.forename: Di
authors.initials: D
authors.lastname: Dong
authors.forename: Shouping
authors.initials: S
authors.lastname: Zhu
authors.forename: Chenghu
authors.initials: C
authors.lastname: Qin
authors.forename: V
authors.initials: V
authors.lastname: Kumar
authors.forename: J V
authors.initials: JV
authors.lastname: Stein
authors.forename: S
authors.initials: S
authors.lastname: Oehler
authors.forename: C
authors.initials: C
authors.lastname: Savakis
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.forename: J
authors.initials: J
authors.lastname: Ripoll
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000033:anatomy & histology; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008198:Lymph Nodes / Q000033:anatomy & histology; D051379:Mice; D019047:Phantoms, Imaging; D011679:Pupa / Q000033:anatomy & histology; D012399:Rotation; D012542:Scattering, Radiation; D041622:Tomography, Optical / Q000295:instrumentation / Q000379:methods*