title: Development of high-performance liquid chromatography-time-of-flight mass spectrometry for the simultaneous characterization and quantitative analysis of gingerol-related compounds in ginger products.
abstract: Liquid chromatography-time-of-flight mass spectrometry (LC-TOF/MS) was established for the simultaneous separation, identification, and quantification of gingerol-related compounds in ginger products. The established method has been shown to provide a satisfactory linearity (r > 0.999) in a wide range (5-5000 ng/mL), low limits of detection and quantification, high precision, and inter- and intraday repeatability. The detection sensitivity of gingerols and shogaols by TOF/MS was 70-100 times higher than conventional UV detection at 288 nm. In this study, 19 ginerol-related compounds in the samples were identified and quantified by the established LC-TOF/MS method. The dried ginger powder products contained the highest quantity of gingerol-related compounds (7126.3-13789.0 μg/g), followed by fresh ginger products (2007.9-2790.0 μg/g), powdered ginger tea products (77.29-81.75 μg/g), and hot water ginger extracts (54.59-123.23 μg/mL). Shogaols were not found in fresh gingers. This paper represents the first report on the LC-TOF/MS analysis for the simultaneous characterization and quantification of gingerol-related compounds in ginger products.
authors.affiliation: College of Food Science, Woosuk University , Samrea-Up, Wanju-Kun, Jeonbuk Province 565-701, Republic of Korea.
authors.forename: Ji Su
authors.initials: JS
authors.lastname: Park
authors.forename: Mun Yhung
authors.initials: MY
authors.lastname: Jung
mesh_terms: D002396:Catechols / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005233:Fatty Alcohols / Q000032:analysis* / Q000302:isolation & purification; D020939:Zingiber officinale / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods
chemical_list: D002396:Catechols; D005233:Fatty Alcohols; D010936:Plant Extracts; C040115:shogaol; C007845:gingerol