title: A sandwich-culture technique for controlling antibiotic production and morphological development in Streptomyces coelicolor.
abstract: Antibiotic-producing streptomycetes form complex colonies consisting of vegetative and aerial hyphae. Here, we describe a sandwich-culture technique in which the mycelium grows in between two membranes, thereby preventing morphogenesis and antibiotic production. Both processes are restored by removal of the top membrane, thus providing a switch to coordinate their timing.
authors.affiliation: Molecular Biotechnology, Institute Biology Leiden, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands.
authors.forename: Inez P
authors.initials: IP
authors.lastname: de Jong
authors.forename: Dennis
authors.initials: D
authors.lastname: Claessen
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D019149:Bioreactors / Q000382:microbiology; D007218:Industrial Microbiology / Q000379:methods*; D048372:Streptomyces coelicolor / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents