title: Improvement of a puromycin-linker to extend the selection target varieties in cDNA display method.
abstract: cDNA display using a puromycin-linker to covalently bridge a protein and its coding cDNA is a stable and efficient in vitro protein selection method. The optimal design of the often-used puromycin-linker is vital for effective selection. In this report, an improved puromycin-linker containing deoxyinosine bases as cleavage sites, which are recognized by endonuclease V, was introduced to extend the variety of the selection targets to molecules such as RNA. The introduction of this linker enables efficient in vitro protein selection without contamination from RNase T1, which is used for the conventional linker containing ribonucleotide G bases. In addition, mRNA-protein fusion efficiency was found to not depend on the length of the flexible poly (ethylene glycol) (PEG) region of the linker. These findings will allow practical and easy-to-use in vitro protein selection by cDNA display.
authors.affiliation: Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo, Saitama 338-8570, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Ueno
authors.forename: Shinnosuke
authors.initials: S
authors.lastname: Kimura
authors.forename: Takanori
authors.initials: T
authors.lastname: Ichiki
authors.forename: Naoto
authors.initials: N
authors.lastname: Nemoto
mesh_terms: D001709:Biotechnology; D018076:DNA, Complementary / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019020:Directed Molecular Evolution / Q000379:methods*; D015723:Gene Library; D011092:Polyethylene Glycols / Q000737:chemistry; D015202:Protein Engineering / Q000379:methods*; D011691:Puromycin / Q000737:chemistry* / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D011092:Polyethylene Glycols; D011691:Puromycin