title: Eilatimonas milleporae gen. nov., sp. nov., a marine bacterium isolated from the hydrocoral Millepora dichotoma.
abstract: A marine bacterial strain, designated MD2(T), was isolated from the damaged tissue of a hydrocoral, Millepora dichotoma, collected from the coral reef in the northern Red Sea, Gulf of Eilat, Israel. Strain MD2(T) was Gram-reaction-negative, rod-shaped and motile, and formed small, creamy and opaque colonies, 1-2 mm in diameter, after 3 days incubation on Marine agar at 30°C. The novel strain grew well in nutrient broth at 1.5-6 % NaCl and at 20-37°C. The major cellular fatty acids were iso-C17 : 1ω9c, iso-C17 : 0, C18 : 1ω7c and C17 : 1ω6c. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, an unidentified lipid, two unidentified phospholipids, two unidentified glycolipids and two unidentified aminolipids. Ubiquinone Q-10 was the only respiratory lipoquinone. The DNA G+C content was 60.3 mol%. Analysis of the 16S rRNA gene sequence placed the organism in the α-subclass of the Proteobacteria with a sequence divergence of about 9 % from any species with a validly published name. The highest 16S rRNA gene sequence similarity (approximately 91 %) was notably with type strains of members of the genus Kordiimonas, Kordiimonas aestuarii 101-1(T), Kordiimonas lacus S3-22(T) and Kordiimonas gwangyangensis GW14-5(T). On the basis of genotypic, chemotaxonomic and phenotypic distinctness, strain MD2(T) represents a novel species in a new genus of the class Alphaproteobacteria, for which the name Eilatimonas milleporae gen. nov., sp. nov. is proposed. The type strain of the type species is MD2(T) ( = LMG 26586(T) = DSM 25217(T)).
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, PO Box 653, Be'er-Sheva 84105, Israel.
authors.forename: Nithyanand
authors.initials: N
authors.lastname: Paramasivam
authors.affiliation: National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, PO Box 653, Be'er-Sheva 84105, Israel.|Achva Academic College, M.P. Shikmim 79800, Israel.
authors.forename: Eitan
authors.initials: E
authors.lastname: Ben-Dov
authors.affiliation: Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, PO Box 653, Be'er-Sheva 84105, Israel.
authors.forename: Luba
authors.initials: L
authors.lastname: Arotsker
authors.affiliation: School of Materials Science and Engineering, Nanyang Technological University, Singapore 637819, Singapore.|National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, PO Box 653, Be'er-Sheva 84105, Israel.|Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, PO Box 653, Be'er-Sheva 84105, Israel.
authors.forename: Ariel
authors.initials: A
authors.lastname: Kushmaro
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019156:Indian Ocean; D007557:Israel; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2