title: Endothelial differentiation in multipotent cells derived from mouse and human white mature adipocytes.
abstract: White mature adipocytes give rise to multipotent cells, so-called de-differentiated fat (DFAT) cells, when losing their fat in culture. The objective of this study was to examine the ability of DFAT cells to give rise to endothelial cells (ECs) in vitro and vivo. We demonstrate that mouse and human DFAT cells, derived from adipose tissue and lipospirate, respectively, initially lack expression of CD34, CD31, CD146, CD45 and pericyte markers, distinguishing them from progenitor cells previously identified in adipose stroma. The DFAT cells spontaneously differentiate into vascular ECs in vitro, as determined by real-time PCR, fluorescence activated cell sorting, immunostaining, and formation of tube structures. Treatment with bone morphogenetic protein (BMP)4 and BMP9, important in regulating angiogenesis, significantly enhances the EC differentiation. Furthermore, adipocyte-derived cells from Green Fluorescent Protein-transgenic mice were detected in the vasculature of infarcted myocardium up to 6 weeks after ligation of the left anterior descending artery in mice. We conclude that adipocyte-derived multipotent cells are able to spontaneously give rise to ECs, a process that is promoted by BMPs and may be important in cardiovascular regeneration and in physiological and pathological changes in fat and other tissues.
authors.affiliation: Division of Cardiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1679, USA.
authors.forename: Medet
authors.initials: M
authors.lastname: Jumabay
authors.forename: Raushan
authors.initials: R
authors.lastname: Abdmaulen
authors.forename: Sumithra
authors.initials: S
authors.lastname: Urs
authors.forename: Sepideh
authors.initials: S
authors.lastname: Heydarkhan-Hagvall
authors.forename: Gregorio D
authors.initials: GD
authors.lastname: Chazenbalk
authors.forename: Maria C
authors.initials: MC
authors.lastname: Jordan
authors.forename: Kenneth P
authors.initials: KP
authors.lastname: Roos
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Yao
authors.forename: Kristina I
authors.initials: KI
authors.lastname: Boström
mesh_terms: D052438:Adipocytes, White / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000494:pharmacology; D054337:Cell Dedifferentiation / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D015870:Gene Expression; D055412:Growth Differentiation Factors / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000187:drug effects; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy; D033581:Stem Cell Transplantation
chemical_list: D055415:Bone Morphogenetic Protein 4; D055412:Growth Differentiation Factors