title: Molecular characterisation of dengue virus type 1 reveals lineage replacement during circulation in Brazilian territory.
abstract: Dengue fever is the most important arbovirus infection found in tropical regions around the world. Dispersal of the vector and an increase in migratory flow between countries have led to large epidemics and severe clinical outcomes, such as dengue haemorrhagic fever and dengue shock syndrome. This study analysed the genetic variability of the dengue virus serotype 1 (DENV-1) in Brazil with regard to the full-length structural genes C/prM/M/E among 34 strains isolated during epidemics that occurred in the country between 1994-2011. Virus phylogeny and time of divergence were also evaluated with only the E gene of the strains isolated from 1994-2008. An analysis of amino acid differences between these strains and the French Guiana strain (FGA/89) revealed the presence of important nonsynonymous substitutions in the amino acid sequences, including residues E297 (Met→Thr) and E338 (Ser→Leu). A phylogenetic analysis of E proteins comparing the studied isolates and other strains selected from the GenBank database showed that the Brazilian DENV-1 strains since 1982 belonged to genotype V. This analysis also showed that different introductions of strains from the 1990s represented lineage replacement, with the identification of three lineages that cluster all isolates from the Americas. An analysis of the divergence time of DENV-1 indicated that the lineage circulating in Brazil emerged from an ancestral lineage that originated approximately 44.35 years ago.
authors.affiliation: Seção de Arbovirologia e Febres Hemorrágicas, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Ananindeua, PA, Brasil.
authors.forename: Adriana Ribeiro
authors.initials: AR
authors.lastname: Carneiro
authors.forename: Ana Cecília Ribeiro
authors.initials: AC
authors.lastname: Cruz
authors.forename: Marcelo
authors.initials: M
authors.lastname: Vallinoto
authors.forename: Diego de Vasconcelos
authors.initials: Dde V
authors.lastname: Melo
authors.forename: Rommel Thiago J
authors.initials: RT
authors.lastname: Ramos
authors.forename: Daniele Barbosa Almeida
authors.initials: DB
authors.lastname: Medeiros
authors.forename: Eliana Vieira Pinto da
authors.initials: EV
authors.lastname: Silva
authors.forename: Pedro Fernando da Costa
authors.initials: PF
authors.lastname: Vasconcelos
mesh_terms: D000595:Amino Acid Sequence; D001938:Brazil; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D012367:RNA, Viral