title: DEC-205 is a cell surface receptor for CpG oligonucleotides.
abstract: Synthetic CpG oligonucleotides (ODN) have potent immunostimulatory properties exploited in clinical vaccine trials. How CpG ODN are captured and delivered to the intracellular receptor TLR9, however, has been elusive. Here we show that DEC-205, a multilectin receptor expressed by a variety of cells, is a receptor for CpG ODN. When CpG ODN are used as an adjuvant, mice deficient in DEC-205 have impaired dendritic cell (DC) and B-cell maturation, are unable to make some cytokines such as IL-12, and display suboptimal cytotoxic T-cell responses. We reveal that DEC-205 directly binds class B CpG ODN and enhances their uptake. The CpG-ODN binding function of DEC-205 is conserved between mouse and man, although human DEC-205 preferentially binds a specific class B CpG ODN that has been selected for human clinical trials. Our findings identify an important receptor for class B CpG ODN and reveal a unique function for DEC-205.
authors.affiliation: Centre for Immunology,  Burnet Institute, Melbourne, VIC 3004,  Australia.
authors.forename: Mireille H
authors.initials: MH
authors.lastname: Lahoud
authors.forename: Fatma
authors.initials: F
authors.lastname: Ahmet
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Zhang
authors.forename: Simone
authors.initials: S
authors.lastname: Meuter
authors.forename: Antonia N
authors.initials: AN
authors.lastname: Policheni
authors.forename: Susie
authors.initials: S
authors.lastname: Kitsoulis
authors.forename: Chin-Nien
authors.initials: CN
authors.lastname: Lee
authors.forename: Meredith
authors.initials: M
authors.lastname: O'Keeffe
authors.forename: Lucy C
authors.initials: LC
authors.lastname: Sullivan
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Brooks
authors.forename: Richard
authors.initials: R
authors.lastname: Berry
authors.forename: Jamie
authors.initials: J
authors.lastname: Rossjohn
authors.forename: Justine D
authors.initials: JD
authors.lastname: Mintern
authors.forename: Javier
authors.initials: J
authors.lastname: Vega-Ramos
authors.forename: Jose A
authors.initials: JA
authors.lastname: Villadangos
authors.forename: Nicos A
authors.initials: NA
authors.lastname: Nicola
authors.forename: Michel C
authors.initials: MC
authors.lastname: Nussenzweig
authors.forename: Katryn J
authors.initials: KJ
authors.lastname: Stacey
authors.forename: Ken
authors.initials: K
authors.lastname: Shortman
authors.forename: William R
authors.initials: WR
authors.lastname: Heath
authors.forename: Irina
authors.initials: I
authors.lastname: Caminschi
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism*; D016466:CHO Cells; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D016207:Cytokines / Q000097:blood; D003713:Dendritic Cells / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D015778:Minor Histocompatibility Antigens; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D020349:Surface Plasmon Resonance
chemical_list: D015703:Antigens, CD; D016207:Cytokines; C093569:DEC-205 receptor; D037181:Lectins, C-Type; D015778:Minor Histocompatibility Antigens; D009838:Oligodeoxyribonucleotides; D011956:Receptors, Cell Surface