title: Genetically encoded cyclopropene directs rapid, photoclick-chemistry-mediated protein labeling in mammalian cells.
abstract: We just click: Genetic incorporation of a cyclopropene amino acid CpK (see scheme) site-specifically into proteins in E. coli and mammalian cells was achieved using an orthogonal aminoacyl-tRNA synthetase/tRNA(CUA) pair (CpKRS/MbtRNA(CUA)). Cyclopropene exhibited fast reaction kinetics in the photoclick reaction and allowed rapid (ca. 2 min) labeling of proteins.
authors.affiliation: Department of Chemistry, State University of New York at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Yu
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Pan
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Wang
authors.forename: Jiangyun
authors.initials: J
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry / Q000235:genetics; D000604:Amino Acyl-tRNA Synthetases / Q000737:chemistry / Q000235:genetics; D000818:Animals; D019843:Archaeal Proteins / Q000737:chemistry / Q000235:genetics; D057930:Click Chemistry / Q000379:methods*; D003521:Cyclopropanes / Q000737:chemistry*; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives / Q000737:chemistry / Q000235:genetics; D008322:Mammals; D017021:Methanosarcina barkeri / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D012345:RNA, Transfer, Amino Acid-Specific / Q000737:chemistry / Q000235:genetics
chemical_list: D000596:Amino Acids; D019843:Archaeal Proteins; D003521:Cyclopropanes; D012345:RNA, Transfer, Amino Acid-Specific; C021985:cyclopropene; D000604:Amino Acyl-tRNA Synthetases; C456839:pyrrolysine; D008239:Lysine