title: Presence of metastasis-associated protein 1 in Sertoli cells is required for proper contact between Sertoli cells and adjacent germ cells.
abstract: OBJECTIVE
  To investigate whether the normal expression of metastasis-associated protein 1 (MTA1) in Sertoli cells (SCs) is associated with adjacent germ cells (GCs) and to provide the functional relevance of MTA1 in this somatic cell.
  
  
  METHODS
  The expression pattern of MTA1 in the SCs of impaired human spermatogenesis was determined using immunohistochemistry. The effect of the depletion of GCs on the expression of MTA1 in isolated SCs was evaluated using reverse transcriptase polymerase chain reaction in murine testes treated with busulphan. Finally, using multiple assays, the functional investigation of MTA1 by its specific knockdown was performed in SC-GC co-cultures.
  
  
  RESULTS
  SCs were negatively immunolabeled in the tubules with impaired spermatogenesis. Depletion of murine GCs by treatment with busulphan resulted in a dramatic decrease of the MTA1 transcripts level in the isolated SCs on the 15th day of treatment and thereafter had totally abolished MTA1 expression by the 30th day of treatment, respectively. The addition of isolated round spermatids into SC culture could partially elevate MTA1 expression in the latter. Furthermore, MTA1 is crucial to maintain the GC nursery function and normal anchoring junction formation in SCs because ablation of MTA1 by siRNA induced extensive defects of genes related to SC homeostasis.
  
  
  CONCLUSIONS
  We propose a novel role for SC-expressing MTA1, which is determined by the presence of surrounding GCs, in mediating the crosstalk between SCs and GCs by influencing a broad spectrum of gene changes.
authors.affiliation: Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Sun
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Chuchao
authors.initials: C
authors.lastname: Zhu
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D022005:Adherens Junctions / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000477:Alkylating Agents / Q000494:pharmacology; D000818:Animals; D053713:Azoospermia / Q000151:congenital; D002066:Busulfan / Q000494:pharmacology; D020864:Calcium Channels, N-Type; D020865:Calcium Channels, P-Type / Q000235:genetics; D020907:Calcium Channels, Q-Type / Q000235:genetics; D002450:Cell Communication; D018920:Coculture Techniques; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D055785:Gene Knockdown Techniques; D005854:Germ Cells / Q000187:drug effects / Q000378:metabolism*; D053823:Hedgehog Proteins / Q000235:genetics; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics; D009845:Oligospermia / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D054331:Sertoli Cell-Only Syndrome / Q000378:metabolism; D012708:Sertoli Cells / Q000378:metabolism*; D013091:Spermatogenesis; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism*; D015534:Trans-Activators; D014158:Transcription, Genetic; D014168:Transferrin / Q000235:genetics; D055815:Young Adult
chemical_list: D000477:Alkylating Agents; D002066:Busulfan; D020864:Calcium Channels, N-Type; D020865:Calcium Channels, P-Type; D020907:Calcium Channels, Q-Type; D050556:Fatty Acid-Binding Proteins; D053823:Hedgehog Proteins; D006655:Histone Deacetylases; D019950:Mitogen-Activated Protein Kinase 1; D009363:Neoplasm Proteins; D051057:Proto-Oncogene Proteins c-akt; D012097:Repressor Proteins; D015534:Trans-Activators; D014168:Transferrin; C082667:Fabp5 protein, mouse; C089320:MTA1 protein, human