title: The in vivo antimalarial activity of methylene blue combined with pyrimethamine, chloroquine and quinine.
abstract: The effectiveness of methylene blue (MB) combined with pyrimethamine (PYR), chloroquine (CQ) or quinine (Q) was examined in a classical four-day suppressive test against a causative agent of rodent malaria, Plasmodium berghei. A marked potentiation was observed when MB was administered at a non-curative dose of 15 mg/kg/day in combination with PYR (0.19 mg/kg/day) or Q (25 mg/kg/day). No synergy was found between MB (15 mg/Kg) and CQ (0.75 mg/Kg). Our results suggest that the combination of MB with PYR or Q may improve the efficacy of these currently used antimalarial drugs.
authors.affiliation: Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, Colombia.
authors.forename: Giovanny
authors.initials: G
authors.lastname: Garavito
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bertani
authors.forename: Miguel
authors.initials: M
authors.lastname: Quiliano
authors.forename: Alexis
authors.initials: A
authors.lastname: Valentin
authors.forename: Ignacio
authors.initials: I
authors.lastname: Aldana
authors.forename: Eric
authors.initials: E
authors.lastname: Deharo
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000627:therapeutic use*; D002738:Chloroquine / Q000627:therapeutic use; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination / Q000379:methods; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D008288:Malaria / Q000188:drug therapy*; D008297:Male; D008751:Methylene Blue / Q000627:therapeutic use*; D051379:Mice; D010962:Plasmodium berghei / Q000187:drug effects; D011739:Pyrimethamine / Q000627:therapeutic use; D011803:Quinine / Q000627:therapeutic use
chemical_list: D000962:Antimalarials; D004791:Enzyme Inhibitors; D002738:Chloroquine; D011803:Quinine; D008751:Methylene Blue; D011739:Pyrimethamine