title: Proteomic analysis of mitochondria from infantile hemangioma endothelial cells treated with sodium morrhuate and its liposomal formulation.
abstract: Hemangioma is the most common benign tumor of infancy. The aim of this study is to evaluate the biological effects of sodium morrhuate (SM) and its liposomal formulation on infantile hemangioma endothelial cells (IHECs). Morphological analysis revealed that exposure to liposomal sodium morrhuate (LSM) preferentially caused apoptotic death in IHECs, manifested as shrunken configuration and formation of apoptotic bodies. In contrast, necrotic death was prominent in IHECs treated with an equal concentration of SM. By means of proteomic analysis and confirmation experiments, we revealed that the apoptosis-inducing effects of LSM were associated with an upregulation of a set of genes involved in mitochondrial death pathway, including apoptosis-inducing factor, cytochrome c1, caspase-8, and lamin B1. In conclusion, our data highlight the proapoptotic activity of LSM in IHECs through the mitochondrial apoptotic pathway and may provide a promising avenue to treat hemangiomas of infancy.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Xi'an Jiaotong University, Xi'an 710004, People's Republic of China. tujunbo@mail.xjtu.edu.cn
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Tu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Dong
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Hu
authors.forename: Fei
authors.initials: F
authors.lastname: Jiang
authors.forename: Rui-Zhao
authors.initials: RZ
authors.lastname: Ma
authors.forename: Lin-Ying
authors.initials: LY
authors.lastname: He
authors.forename: Zhuang-Qun
authors.initials: ZQ
authors.lastname: Yang
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D051033:Apoptosis Inducing Factor / Q000235:genetics / Q000378:metabolism; D053181:Caspase 8 / Q000235:genetics / Q000378:metabolism; D048430:Cell Shape; D003575:Cytochromes c1 / Q000235:genetics / Q000378:metabolism; D004339:Drug Compounding; D042783:Endothelial Cells / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D006391:Hemangioma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007223:Infant; D034921:Lamin Type B / Q000235:genetics / Q000378:metabolism; D008081:Liposomes; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012976:Sodium Morrhuate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: C494829:AIFM1 protein, human; D000970:Antineoplastic Agents; D051033:Apoptosis Inducing Factor; D034921:Lamin Type B; D008081:Liposomes; D024101:Mitochondrial Proteins; D020543:Proteome; D012976:Sodium Morrhuate; D003575:Cytochromes c1; C505530:CASP8 protein, human; D053181:Caspase 8