title: [Preparation and identification of a recombinant hepatitis C virus (HCV) based on sindbis virus vector].
abstract: OBJECTIVE
  To construct the recombinant virus-like particles containing HCV envelope glycoprotein E1E2 based on sindbis virus vector.
  
  
  METHODS
  The gene encoding HCV envelope glycoprotein E1E2 was cloned into sindbis virus vector to construct recombinant plasmids pBR-XJE1E2 and pVA-XJE1E2, and transfect them into BHK-21 cells to obtain recombinant virus-like particles. The expression of E1 and E2 protein were verified by Western Blot and indirect immunofluorescent assay (IFA).
  
  
  RESULTS
  The results of restriction enzyme digestion, PCR and sequencing analysis showed that the recombinant plasmids were constructed successfully. And the results of RT-PCR, Western blotting and IFA detection showed that the transfect cells could package HCV-like particles of expressing structural proteins E1E2.
  
  
  CONCLUSIONS
  The recombinant expression plasmids pBR-XJE1E2 and pVA-XJE1E2 based on sindbis virus vector could package HCV-like particles in eukaryotic cell, which provides a foundation for further study of its in vivo animal immune response.
authors.affiliation: Kunming Medical College, Kunming 650500, China.
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Fu
authors.forename: Wu-Yang
authors.initials: WY
authors.lastname: Zhu
authors.forename: Jiang-Jiao
authors.initials: JJ
authors.lastname: Li
authors.forename: Huan-Qin
authors.initials: HQ
authors.lastname: Wang
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Tan
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Yin
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D005822:Genetic Vectors; D016174:Hepacivirus / Q000235:genetics*; D010957:Plasmids; D011995:Recombination, Genetic; D012845:Sindbis Virus / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: C087035:E1 protein, Hepatitis C virus; D014759:Viral Envelope Proteins; C091015:glycoprotein E2, Hepatitis C virus