title: Cellular imaging using biocompatible dendrimer-functionalized graphene oxide-based fluorescent probe anchored with magnetic nanoparticles.
abstract: We describe a novel multicomponent graphene nanostructured system that is biocompatible, and has strong NIR optical absorbance and superparamagnetic properties. The fabrication of the multicomponent nanostructure system involves the covalent attachment of 3 components; Fe(3)O(4)(Fe) nanoparticles, PAMAM-G4-NH(2) (G4) dendrimer and Cy5 (Cy) on a graphene oxide (GO) surface to synthesize a biologically relevant multifunctional system. The resultant GO-G4-Fe-Cy nanosystem exhibits high dispersion in an aqueous medium, and is magnetically responsive and fluorescent. In vitro experiments provide a clear indication of successful uptake of the GO-G4-Fe-Cy nanosystem by MCF-7 breast cancer cells, and it is seen to behave as a bright and stable fluorescent marker. The study also reveals varied cellular distribution kinetics profile for the GO nanostructured system compared to free Cy. Furthermore, the newly developed GO nanostructured system is observed to be non-toxic to MDA-MB-231 cell growth, in striking contrast to free G4 dendrimer and GO-G4 conjugate. The GO-G4-Fe-Cy nanostructured system characterized by multifunctionality suggests the merits of graphene for cellular bioimaging and the delivery of bioactives.
authors.affiliation: NCE-Polymer Chemistry Group, Piramal Healthcare Ltd, Goregaon, Mumbai-400063, India.
authors.forename: Prateek S
authors.initials: PS
authors.lastname: Wate
authors.forename: Shashwat S
authors.initials: SS
authors.lastname: Banerjee
authors.forename: Archana
authors.initials: A
authors.lastname: Jalota-Badhwar
authors.forename: Russel R
authors.initials: RR
authors.lastname: Mascarenhas
authors.forename: Khushbu R
authors.initials: KR
authors.lastname: Zope
authors.forename: Jayant
authors.initials: J
authors.lastname: Khandare
authors.forename: R Devesh K
authors.initials: RD
authors.lastname: Misra
mesh_terms: D001943:Breast Neoplasms / Q000175:diagnosis*; D002232:Carbocyanines / Q000737:chemistry / Q000493:pharmacokinetics; D045744:Cell Line, Tumor; D002470:Cell Survival; D050091:Dendrimers / Q000737:chemistry / Q000493:pharmacokinetics; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics; D006108:Graphite / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry; D018613:Microscopy, Confocal; D008958:Models, Molecular; D009757:Nylons / Q000737:chemistry / Q000493:pharmacokinetics; D061848:Optical Imaging
chemical_list: D002232:Carbocyanines; D050091:Dendrimers; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles; D009757:Nylons; C520550:PAMAM-G4; C085321:cyanine dye 5; D006108:Graphite