title: The potential mechanism of Bursal-derived BPP-II on the antibody production and avian pre-B cell.
abstract: The bursa of Fabricius is critical for the normal development of the B lymphocytes responsible for antibody production. However, the mechanism of the bursal-derived bioactive factor on B cell development is little reported. In this paper, chicks were immunized with BPP-II and AIV vaccine or AIV antigen, and antibody and IL-4 production were detected. The results showed that BPP-II played strongly inducing roles on the humoral immune responses. To investigate the gene expression at transcriptional level, avian pre-B lymphocyte DT40 cells were treated with BPP-II, and were analyzed with the gene microarray. The results proved that BPP-II treatment regulated 11 pathways, in which homologous recombination is a vital mechanism which is involved in antibody Ig gene conversion and diversification during B cell development. These results suggested Bursal-derived biological active factor BPP-II might be involved in the antibody production processes and B cell development, which is vital to the humoral central immune organ, the bursa of Fabricius.
authors.affiliation: Division of Key Lab of Animal Disease Diagnosis and Immunology of China's Department of Agriculture, College of Veterinary medicine, Nanjing Agriculture University, Nanjing 210095, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Feng
authors.forename: Ruibing
authors.initials: R
authors.lastname: Cao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Qingtao
authors.initials: Q
authors.lastname: Liu
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Liu
authors.forename: Yuanpeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jinyan
authors.initials: J
authors.lastname: Gu
authors.forename: Denian
authors.initials: D
authors.lastname: Miao
authors.forename: Puyan
authors.initials: P
authors.lastname: Chen
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002645:Chickens; D020869:Gene Expression Profiling; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000276:immunology; D015847:Interleukin-4 / Q000378:metabolism*; D046228:Microarray Analysis; D009842:Oligopeptides / Q000276:immunology*; D054448:Precursor Cells, B-Lymphoid / Q000276:immunology*
chemical_list: D007252:Influenza Vaccines; D009842:Oligopeptides; C006791:bradykinin potentiating factors; D015847:Interleukin-4