title: [Metabonomic study of intervention effects of Morinda officinalis on 'kidney-yang deficiency syndrome'].
abstract: OBJECTIVE
  To investigate the metabolic profile of hydrocortisone-induced 'Kidney-yang deficiency syndrome'in rats and the intervention effects of Morinda officinalis.
  
  
  METHODS
  Proton nuclear magnetic resonance (1H-NMR) technique was used to analyze the rat metabonome in serum. Orthogonal partial least squares discriminant analysis (OPLS-DA) were processed to analyze the metabonome difference between the control and hydrocortisone treated samples. Twelve potential biomarkers were selected, via the parameter of variable importance in the projection (VIP). Principal components analysis (PCA) was employed to process the data from the M. officinalis. treatment group and the intervention effects of M. officinalis, was investigated through the selected potential biomarkers.
  
  
  RESULTS
  After hydrocortisone treatment, the energy metabolism, amino acids metabolism and gut microflora environment were seriously disturbed and transmethylation was surpressed. M. officinalis could effectively alleviate the disturbance of energy and amino acids metabolism and enhance transmethylation, but could not modulate the gut microflora environment.
  
  
  CONCLUSIONS
  The results obtained suggested that metabonomic studies could better reflect the whole status of metabolism in bio-systems, and could be treated as a potential powerful approach in pharmacological studies and investigation of the essence of 'syndrome' in traditional Chinese medicine.
authors.affiliation: Chinese Medicine College, Guangdong Pharmaceutical University, Guangzhou 510006, China. gongmengjuan@yahoo.com.cn
authors.forename: Mengjuan
authors.initials: M
authors.lastname: Gong
authors.forename: Wenhua
authors.initials: W
authors.lastname: Ye
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xie
authors.forename: Shumei
authors.initials: S
authors.lastname: Wang
authors.forename: Shengwang
authors.initials: S
authors.lastname: Liang
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Zou
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D055432:Metabolomics; D032066:Morinda / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016711:Yang Deficiency / Q000188:drug therapy* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal