title: Application of different chemometric strategies to voltammetric and UV-vis spectroscopic data to obtain a complexation model: study of the Cu(II) binding with the phytohormone 6-benzylaminopurine.
abstract: The complexation processes of Cu(II) by the phytohormone and the possible antitumoral agent 6-benzylaminopurine (BAP) were studied by Differential Pulse Polarography (DPP) and ultraviolet-visible spectroscopy (UV-vis) in combination with chemometric programs such as Multivariate Curve Resolution by Alternating Least-Squares (MCR-ALS) and Gaussian Peak Adjustment (GPA). All data confirm the formation of the predominant 1 : 1 and 1 : 2 Cu(2+) : BAP complexes. The corroboration of the presented stoichiometries was performed by ElectroSpray Ionization-Mass Spectrometric (ESI-MS) experiments of the Cu(II)-BAP system at different ratios.
authors.affiliation: Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, Martí i Franquès 1-11, E-08028 Barcelona, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Cavanillas
authors.forename: Arístides
authors.initials: A
authors.lastname: Alberich
authors.forename: Núria
authors.initials: N
authors.lastname: Serrano
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Díaz-Cruz
authors.forename: Cristina
authors.initials: C
authors.lastname: Ariño
authors.forename: Miquel
authors.initials: M
authors.lastname: Esteban
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D001593:Benzyl Compounds / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D055664:Electrochemical Techniques; D007477:Ions / Q000737:chemistry; D016018:Least-Squares Analysis; D008956:Models, Chemical; D011687:Purines / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D001593:Benzyl Compounds; D007477:Ions; D011687:Purines; D003300:Copper; C480551:benzylaminopurine