title: Characterization of chondrocytes cultured on catechin-loaded alginate-chitosan scaffolds.
abstract: Bovine chondrocytes were seeded into scaffolds of a high molecular weight chitosan and alginate with a pore size of 50-350 μm with or without catechin. In polymerase chain reaction (PCR), unlike type II, collagen type I was no longer expressed at day 14. The DNA content increased until day 8 and began declining, indicating cell detachment. The GAG content increased during the first 12 days. The percentage of round and collagen type II immunoreactive cells increased over the time. Catechin has some protective properties on chondrocytes seeded on the alginate-chitosan scaffolds during the first 12 days by means of DNA and chondrocyte morphology (p < 0.05).
authors.affiliation: Department of Biology, Faculty of Art-science, Kirikkale University, Kirikkale, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Türk
authors.forename: Siyami
authors.initials: S
authors.lastname: Karahan
authors.forename: Miyase
authors.initials: M
authors.lastname: Çinar
authors.forename: Sebnem
authors.initials: S
authors.lastname: Küçük
authors.forename: Güngör Çagdaş
authors.initials: GÇ
authors.lastname: Dinçel
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002392:Catechin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002417:Cattle; D018929:Cell Culture Techniques; D048271:Chitosan / Q000378:metabolism*; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006025:Glycosaminoglycans / Q000378:metabolism; D006603:Hexuronic Acids / Q000737:chemistry; D010641:Phenotype; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D006025:Glycosaminoglycans; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D002392:Catechin; D004247:DNA; D048271:Chitosan