title: Decreased miR-7 expression in the skin and sera of patients with dermatomyositis.
abstract: Expression of microRNA (miRNA) in the skin in dermatomyositis has not previously been studied in detail. In this study, we performed miRNA array analysis using miRNAs purified from dermatomyositis-involved skin and normal skin, and found that several miRNAs were up- or down-regulated in dermatomyositis skin. Among them, we focused on miR-7, one of the most down-regulated miRNAs in dermatomyositis skin. Total miRNAs were purified from serum, and hsa-miR-7 levels were measured with quantitative real-time PCR using the specific primer. Serum levels of miR-7 were significantly decreased in patients with dermatomyositis compared with normal subjects or patients with other autoimmune diseases. Thus, serum miR-7 levels might be a possible diagnostic marker for dermatomyositis. Clarifying the up- or down-stream events of down-regulated miR-7 in patients with dermatomyositis may lead to further understanding of the disease and a new therapeutic approach.
authors.affiliation: Department of Dermatology and Plastic Surgery, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Oshikawa
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Jinnin
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Makino
authors.forename: Ikko
authors.initials: I
authors.lastname: Kajihara
authors.forename: Katsunari
authors.initials: K
authors.lastname: Makino
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Honda
authors.forename: Wakana
authors.initials: W
authors.lastname: Nakayama
authors.forename: Kuniko
authors.initials: K
authors.lastname: Inoue
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukushima
authors.forename: Hironobu
authors.initials: H
authors.lastname: Ihn
mesh_terms: D016022:Case-Control Studies; D003882:Dermatomyositis / Q000097:blood / Q000235:genetics*; D015536:Down-Regulation; D020869:Gene Expression Profiling / Q000379:methods; D005819:Genetic Markers; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000097:blood; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000737:chemistry*
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs; C549242:MIRN7-1 microRNA, human