title: Family-based and case-control study of glutamate receptor GRIK3 Ser310Ala polymorphism in alcohol dependence.
abstract: OBJECTIVE
  The aim of this study was to evaluate the role of the glutamate receptor subunit-7 (GluR7, GRIK 3) rs6691840 (Ser310Ala, T928G) in the pathogenesis of alcohol dependence (AD).
  
  
  METHODS
  DNA was provided from AD patients (n = 209) and healthy control subjects (n = 308) all of Polish descent. The history of alcoholism was obtained using the Polish version of the SSAGA (Semi-Structured Assessment for the Genetics of Alcoholism). We conducted case-control association study and transmission disequilibrium test (TDT). GRIK3 functional polymorphism was genotyped by the PCR-RFLP method.
  
  
  RESULTS
  Analyses revealed that polymorphism Ser310Ala of GRIK3 gene is not associated with AD or any of its subgroups. TDT reveled an adequate transmission of both alleles in the group of alcohol families.
  
  
  CONCLUSIONS
  These findings replicate and extend our previous research results that do not support the hypothesis of the role of rs6691840 in the pathogenesis of AD.
authors.affiliation: Department of Psychiatry, Pomeranian Medical University, Szczecin, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Grzywacz
authors.forename: I
authors.initials: I
authors.lastname: Małecka
authors.forename: A
authors.initials: A
authors.lastname: Suchanecka
authors.forename: P
authors.initials: P
authors.lastname: Bieńkowski
authors.forename: J
authors.initials: J
authors.lastname: Samochowiec
mesh_terms: D000328:Adult; D000437:Alcoholism / Q000235:genetics*; D000483:Alleles; D016022:Case-Control Studies; D005190:Family; D005260:Female; D056726:Genetic Association Studies / Q000379:methods / Q000706:statistics & numerical data; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018092:Kainic Acid Receptors / Q000235:genetics*; D000097808:GluK3 Kainate Receptor
chemical_list: D018092:Kainic Acid Receptors; D000097808:GluK3 Kainate Receptor