title: PELP1/MNAR suppression inhibits proliferation and metastasis of endometrial             carcinoma cells.
abstract: Proline-, glutamic acid- and leucine-rich protein-1/modulator of non-genomic             activity of estrogen receptor (ER) (PELP1/MNAR) is a novel nuclear receptor (NR)             co-activator that plays an essential role in the actions of ER. Emerging findings             suggest that PELP1/MNAR is a novel proto-oncogene, whose expression is deregulated             in several hormone-responsive cancers, including endometrial cancer. In this study,             we demonstrate that PELP1/MNAR is widely expressed in endometrial carcinoma cell             lines. To investigate its possible role in endometrial carcinoma progression,             we adopted an RNA interference technology to downregulate PELP1/MNAR expression             in Ishikawa endometrial carcinoma cells. PELP1/MNAR downregulation substantially             reduced cell proliferation, and the cells in which PELP1/MNAR expression was knocked             down also exhibited a decreased migration and invasion ability, as shown by Boyden             chamber and invasion assays. The results showed that the expression of MMP-2 and             MMP-9 was also decreased. These results suggest that PELP1/MNAR plays a role in             endometrial cancer progression and metastasis, and that PELP1/MNAR may be a potential             therapeutic target for endometrial cancer.
authors.affiliation: Department of Gynecology and Obstetrics, The Third Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510630, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wan
authors.forename: Xiaomao
authors.initials: X
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D056970:Co-Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis / Q000235:genetics; D000090063:Proto-Oncogene Mas; D034622:RNA Interference; D034741:RNA, Small Interfering; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D056970:Co-Repressor Proteins; C000715470:MAS1 protein, human; C438485:PELP1 protein, human; D000090063:Proto-Oncogene Mas; D034741:RNA, Small Interfering; D014157:Transcription Factors; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9