title: [EMS mutation of suspension cells and selection of high temperature tolerant mutants from Pinellia ternata].
abstract: OBJECTIVE
  To determine the optimal concentration and processing time of EMS mutation for suspension cells from Pinellia ternata.
  
  
  METHODS
  Under four EMS concentration gradients (0.1% , 0.2%, 0.4%, 0.6%) and three processing time gradients (0.5, 1.0, 2.0 h), the suspension cells of P. ternata were mutagenized.
  
  
  CONCLUSIONS
  The results showed that the survival rate was significantly different under the different concentrations of EMS and the different processing time. In the same processing time, the EMS concentrations were increased, but the suspension cells survival rate decreased gradually. The optimum EMS concentration for the mutagenesis was 0.4% and the best processing time was 1 hour.
authors.affiliation: School of Life Science, Huaibei Normal University, Anhui Key Laboratory of Plant Resources and Biology, Huaibei 235000, China.
authors.forename: Xingkui
authors.initials: X
authors.lastname: Tao
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhang
authors.forename: Jianping
authors.initials: J
authors.lastname: Xue
authors.forename: Jingtong
authors.initials: J
authors.lastname: Teng
authors.forename: Hedong
authors.initials: H
authors.lastname: Lu
authors.forename: Tao
authors.initials: T
authors.lastname: Xue
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D016296:Mutagenesis / Q000187:drug effects; D009154:Mutation / Q000187:drug effects*; D030021:Pinellia / Q000166:cytology* / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D013535:Suspensions; D013696:Temperature; D013997:Time Factors
chemical_list: D013535:Suspensions; D005020:Ethyl Methanesulfonate