title: MicroRNA expression during neuronal differentiation of human teratocarcinoma NTera2D1 and mouse embryonic carcinoma P19 cells.
abstract: MicroRNAs (miRNAs) are 21-23-nucleotide-long small noncoding RNAs, function as mediators in gene silencing, and play essential roles in gene regulation in development, differentiation, and proliferation. Hundreds of miRNAs have been found, and tissue-specific or organ-specific expression of miRNAs has been detected. Here, I describe procedures for detection of miRNAs in the course of neuronal differentiation of human teratocarcinoma NTera2D1 and mouse embryonic carcinoma P19 cells.
authors.affiliation: National Institute of Neuroscience, NCNP, Kodaira, Tokyo, Japan. hohjohh@ncnp.go.jp
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Hohjoh
mesh_terms: D000818:Animals; D018236:Carcinoma, Embryonal; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D055495:Neurogenesis / Q000235:genetics*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D018243:Teratocarcinoma
chemical_list: D035683:MicroRNAs