title: Synthesis, characterization, and mineralization of polyamide-6/calcium lactate composite nanofibers for bone tissue engineering.
abstract: Polyamide-6 nanofibers containing calcium lactate (CL) on their surface were prepared by neutralization of lactic acid (LA) in core-shell structured polyamide-6/LA electrospun fibers. First, simple blending of LA with polyamide-6 solution was used for electrospinning which interestingly formed a thin LA layer around polyamide-6 nanofibers (core-shell structure) and then subsequent conversion of this LA into calcium lactate via neutralization using calcium base. FE-SEM and TEM images revealed that plasticizer capacity of LA led the formation of point-bonded structure due to the formation of shell layer of LA and core of polyamide-6. The bone formation ability of polyamide-6/calcium lactate composite fibers was evaluated by incubating in biomimetic simulated body fluid (SBF). The SBF incubation test confirmed the faster deposition of large amount of calcium phosphate around the composite polyamide-6/calcium lactate fibers compared to pristine polyamide-6. This study demonstrated a simple post electrospinning calcium compound coating technique of polymeric nanofibers for enhancing the bone biocompatibility of polyamide-6 fibers.
authors.affiliation: Department of Bio-nano System Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea. hempant@jbnu.ac.kr
authors.forename: Hem Raj
authors.initials: HR
authors.lastname: Pant
authors.forename: Prabodh
authors.initials: P
authors.lastname: Risal
authors.forename: Chan Hee
authors.initials: CH
authors.lastname: Park
authors.forename: Leonard D
authors.initials: LD
authors.lastname: Tijing
authors.forename: Yeon Jun
authors.initials: YJ
authors.lastname: Jeong
authors.forename: Cheol Sang
authors.initials: CS
authors.lastname: Kim
mesh_terms: D001672:Biocompatible Materials / Q000009:adverse effects / Q000138:chemical synthesis* / Q000737:chemistry; D001842:Bone and Bones / Q000187:drug effects; D017610:Calcium Compounds / Q000737:chemistry*; D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry; D007773:Lactates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D007773:Lactates; D011108:Polymers; C009916:nylon 6; C110051:calcium lactate; D002209:Caprolactam