title: Two techniques for simultaneous identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri by use of the small-subunit rRNA gene.
abstract: The primers traditionally used to detect Plasmodium ovale infections are known for not binding all P. ovale parasites within the small-subunit rRNA gene when used alone. We describe a simple, cost- and time-efficient multiplex nested PCR and a nested PCR using a novel set of primers for the simultaneous detection of P. ovale curtisi and P. ovale wallikeri.
authors.affiliation: Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Vienna, Austria.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Fuehrer
authors.forename: Marie-Therese
authors.initials: MT
authors.lastname: Stadler
authors.forename: Katharina
authors.initials: K
authors.lastname: Buczolich
authors.forename: Ingrid
authors.initials: I
authors.lastname: Bloeschl
authors.forename: Harald
authors.initials: H
authors.lastname: Noedl
mesh_terms: D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D020459:Genes, rRNA; D006801:Humans; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology; D060885:Multiplex Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D010274:Parasitology / Q000191:economics / Q000379:methods*; D041122:Plasmodium ovale / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D016053:RNA, Protozoan / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D013997:Time Factors
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D016053:RNA, Protozoan; D012337:RNA, Ribosomal, 18S