title: Effects of resveratrol on blood homocysteine level, on homocysteine induced oxidative stress, apoptosis and cognitive dysfunctions in rats.
abstract: We aimed to examine the protective effects of resveratrol against homocysteine induced oxidative stress, apoptosis and cognitive impairment. Rats were randomly divided into three groups. Control group received standard rat food; homocysteine group (Hcy group) received daily methionine at a dose of 1g/kg-body weight dissolved in drinking water for thirty days; third group (Hcy+Res group) received same amount of methionine plus 20mg/kg/day resveratrol intraperitoneally for thirty days. Cognitive performances of the animals were tested by Morris water maze test. Then all animals were sacrificed to study lipid peroxidation (LPO), DNA fragmentation and p53 mRNA expression in the rat brain. The aortas of the sacrificed rats were processed for histopathological examination. Apoptosis in the aortas was assessed by TUNEL staining. Resveratrol significantly decreased serum levels of homocysteine, reversed Hcy induced LPO increase, decreased DNA fragmentation and p53 mRNA expression in the rat brains, and improved homocysteine induced impairment of long term spatial memory. Resveratrol could inhibit homocysteine induced apoptosis and histopathological deterioration in the rat aortic sections. In conclusion, resveratrol is effective in preventing homocysteine induced vascular and neural defects. In hyperhomocysteinemic rat model, our findings consequently warrant in future studies to reveal the true improvement mechanism of resveratrol.
authors.affiliation: Malatya State Hospital, Laboratory Department, 44000 Malatya, Turkey. kozsema@hotmail.com
authors.forename: Sema Tulay
authors.initials: ST
authors.lastname: Koz
authors.forename: Ebru Onalan
authors.initials: EO
authors.lastname: Etem
authors.forename: Gıyasettin
authors.initials: G
authors.lastname: Baydas
authors.forename: Huseyin
authors.initials: H
authors.lastname: Yuce
authors.forename: Halil Ibrahim
authors.initials: HI
authors.lastname: Ozercan
authors.forename: Tuncay
authors.initials: T
authors.lastname: Kuloğlu
authors.forename: Suleyman
authors.initials: S
authors.lastname: Koz
authors.forename: Arzu
authors.initials: A
authors.lastname: Etem
authors.forename: Nevgul
authors.initials: N
authors.lastname: Demir
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001011:Aorta / Q000187:drug effects / Q000473:pathology; D017209:Apoptosis / Q000187:drug effects*; D003072:Cognition Disorders / Q000378:metabolism*; D053938:DNA Fragmentation / Q000187:drug effects; D006710:Homocysteine / Q000097:blood*; D020287:In Situ Nick-End Labeling; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D006710:Homocysteine; D000077185:Resveratrol