title: Tracking the total CD8 T cell response following whole Plasmodium vaccination.
abstract: CD8 T cells are critical mediators of protection against Plasmodium liver-stage infection. Most studies of the CD8 T cell response to whole parasite Plasmodium vaccines address a single T cell epitope in BALB/c mice, and thus provide limited information. Here, we describe a surrogate activation marker approach that uses the coordinate downregulation of the CD8α chain and upregulation of the integrin CD11a to track the total CD8 T cell response to Plasmodium vaccination via flow cytometry. With this approach, quantitative (magnitude, kinetics) and qualitative (distribution, phenotype, and function) features of the total CD8 T cell response to vaccination with attenuated Plasmodium or other pathogens can be studied.
authors.affiliation: Department of Microbiology, University of Iowa, Iowa City, IA, USA.
authors.forename: Katherine L
authors.initials: KL
authors.lastname: Doll
authors.forename: Noah S
authors.initials: NS
authors.lastname: Butler
authors.forename: John T
authors.initials: JT
authors.lastname: Harty
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods; D008099:Liver / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008288:Malaria / Q000276:immunology* / Q000469:parasitology* / Q000517:prevention & control; D017780:Malaria Vaccines / Q000276:immunology*; D051379:Mice; D010961:Plasmodium / Q000276:immunology*; D013154:Spleen / Q000276:immunology
chemical_list: D017780:Malaria Vaccines