title: Langerhans cells in the human tympanic membrane in health and disease:  a morphometric analysis.
abstract: OBJECTIVE
  The normal tympanic membrane contains Langerhans dendritic cells, and they play a role in the pathogenesis of chronic suppurative otitis media.
  
  
  BACKGROUND
  The presence of Langerhans dendritic cells in the normal tympanic membrane is disputed. However, they have been identified in tympanic membranes of patients with otitis media. A quantitative analysis of the distribution and morphology of these cells in the types of chronic suppurative otitis media has not been undertaken.
  
  
  METHODS
  Samples of normal cadaveric tympanic membranes and those from patients with chronic suppurative otitis media of the tubotympanic and atticoantral varieties were stained with the immunohistochemical marker CD1a. The number of cells per unit length of basement membrane, diameters of cells, and number and length of dendritic processes were compared between the groups.
  
  
  RESULTS
  CD1a-positive Langerhans dendritic cells were present in the normal tympanic membrane. The number of cells per unit length of basement membrane, diameters of cells, and the length of dendritic processes increased significantly in tubotympanic disease and in atticoantral disease, the difference being more pronounced in the latter form of otitis media.
  
  
  CONCLUSIONS
  Langerhans cells are present in the normal tympanic membrane, and they probably play differing roles in the pathogenesis of tubotympanic and atticoantral forms of chronic suppurative otitis media.
authors.affiliation: Department of Anatomy, Christian Medical College, Vellore, Tamil Nadu, India. triptimj@gmail.com
authors.forename: Tripti Meriel
authors.initials: TM
authors.lastname: Jacob
authors.forename: Inbam
authors.initials: I
authors.lastname: Indrasingh
authors.forename: Bijesh Kumar
authors.initials: BK
authors.lastname: Yadav
authors.forename: Vedantam
authors.initials: V
authors.lastname: Rupa
mesh_terms: D018949:Antigens, CD1 / Q000737:chemistry; D001485:Basement Membrane / Q000473:pathology / Q000648:ultrastructure; D001706:Biopsy; D002102:Cadaver; D002452:Cell Count; D002908:Chronic Disease; D003713:Dendritic Cells / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007759:Labyrinth Diseases / Q000473:pathology*; D007801:Langerhans Cells / Q000473:pathology / Q000502:physiology / Q000648:ultrastructure*; D010035:Otitis Media, Suppurative / Q000473:pathology; D014021:Tissue Preservation; D014432:Tympanic Membrane / Q000166:cytology* / Q000473:pathology*; D018058:Tympanic Membrane Perforation / Q000473:pathology
chemical_list: D018949:Antigens, CD1; C419393:CD1a antigen