title: Preparation of phytoplasma membrane recombinant proteins.
abstract: The study of phytoplasma membrane protein interactions with host cell proteins is crucial to understand the life cycle of these unculturable microorganisms within their hosts. A step-by-step protocol for the heterologous expression of phytoplasma membrane proteins in Escherichia coli is described, together with a procedure to purify a suitable quantity of fusion antigen for application in the study of phytoplasma-host interactions.
authors.affiliation: Istituto di Virologia Vegetale, CNR, Torino, Italy.
authors.forename: Luciana
authors.initials: L
authors.lastname: Galetto
authors.forename: Majid
authors.initials: M
authors.lastname: Siampour
authors.forename: Cristina
authors.initials: C
authors.lastname: Marzachì
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D045582:Phytoplasma / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012995:Solubility
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins