title: A new validated HPTLC method for quantitative determination of 1, 5-dicaffeoylquinic acid in Inula crithmoides roots.
abstract: 1, 5-Dicaffeoylquinic acid (1, 5-DCQA), a potent HIV-1 integrase inhibitor, is currently undergoing an evaluation as a promising novel HIV therapeutic agent. This work aims at developing an accurate, rapid, repeatable and robust HPTLC method for the determination of 1, 5-DCQA in its natural sources. 1, 5-DCQA is the major component of the n-butanol fraction, the most biologically active hepatoprotective fraction, of Inula crithmoides roots extract. Thus, it will be of interest to evaluate the plant roots as a potential source of 1, 5-DCQA using a fully validated HPTLC method. The percentage of 1, 5-DCQA in the studied plant (0.035% w/w) was found to be approximately similar to those previously determined in other antioxidant herbal drugs, in which 1, 5- DCQA is the main phenolic constituent. The results obtained showed that the described HPTLC method is suitable for routine use in quality control of herbal raw material, extracts and pharmaceutical preparations containing 1, 5-DCQA. No HPTLC method has been reported in literature for the determination of 1, 5-DCQA in medicinal plants.
authors.affiliation: Department of Pharmacognosy and Medicinal Plants, Beirut Arab University, Beirut, Lebanon.
authors.forename: Maha Ahmed
authors.initials: MA
authors.lastname: Aboul-Ela
authors.forename: Abdalla Mohamed
authors.initials: AM
authors.lastname: El-Lakany
authors.forename: Safa Mohamed
authors.initials: SM
authors.lastname: Shams Eldin
authors.forename: Hala Mostafa
authors.initials: HM
authors.lastname: Hammoda
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D002934:Cinnamates / Q000032:analysis*; D019428:HIV Integrase Inhibitors / Q000032:analysis*; D038143:Inula / Q000737:chemistry*; D057230:Limit of Detection; D018517:Plant Roots / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D002934:Cinnamates; D019428:HIV Integrase Inhibitors; C100257:cynarine