title: Detection and quantification of human papillomavirus in benign and malignant parotid lesions.
abstract: OBJECTIVE
  Human papillomavirus (HPV) is implicated in head and neck squamous cell carcinomas. However, the causal role of HPV in carcinomas of the parotid gland remains uncertain and less documented. This study aimed to determine the potential implication of HPV in the development of benign and malignant lesions of the parotid gland.
  
  
  METHODS
  Paraffin-embedded biopsies were obtained from 40 patients with benign parotid gland tumors and from 39 patients with parotid gland carcinomas. The 79 samples were evaluated for the presence of HPV DNA using both GP5+/GP6+ consensus Polymerase Chain Reaction (PCR) and type-specific E6/E7 PCR to detect 18 HPV types.
  
  
  RESULTS
  Our results showed a low prevalence of HPV, with only three HPV-positive cases among the 40 benign tumors and one infected carcinoma in the malignant population.
  
  
  CONCLUSIONS
  No association between the presence of HPV DNA and the development of parotid gland tumors was found in our study.
authors.affiliation: Laboratory of Anatomy, Faculty of Medicine and Pharmacy, University of Mons, Mons, Belgium.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Descamps
authors.forename: Anaëlle
authors.initials: A
authors.lastname: Duray
authors.forename: Alexandra
authors.initials: A
authors.lastname: Rodriguez
authors.forename: Gilbert
authors.initials: G
authors.lastname: Chantrain
authors.forename: Christophe E
authors.initials: CE
authors.lastname: Depuydt
authors.forename: Philippe
authors.initials: P
authors.lastname: Delvenne
authors.forename: Sven
authors.initials: S
authors.lastname: Saussez
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009856:Oncogene Proteins, Viral / Q000235:genetics; D030361:Papillomavirus Infections / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D010307:Parotid Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D055815:Young Adult
chemical_list: C000614131:CDKN2A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral; D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; C052633:E7 protein, Human papillomavirus type 18; D009363:Neoplasm Proteins; D009856:Oncogene Proteins, Viral