title: Effects of multiple-target anti-microRNA antisense oligodeoxyribonucleotides on proliferation and migration of gastric cancer cells.
abstract: BACKGROUND
  To investigate the inhibiting effects of multi-target anti-microRNA antisense oligonucleotide (MTg-AMOs) on proliferation and migration of human gastric cancer cells.
  
  
  METHODS
  Single anti-microRNA antisense oligonucleotides (AMOs) and MTg-AMOs for miR-221, 21, and 106a were designed and transfected into SGC7901, a gastric cancer cell line, to target the activity of these miRNAs. Their expression was analyzed using stem-loop RT-PCR and effects of MTg-AMOs on human gastric cancer cells were determined using the following two assay methods: CCK8 for cell proliferation and transwells for migration.
  
  
  RESULTS
  In the CCK-8 cell proliferation assay, 0.6 μmol/L was selected as the preferred concentration of MTg-AMOs and incubation time was 72 hours. Under these experimental conditions, MTg-AMOs demonstrated better suppression of the expression of miR-221, miR-106a, miR-21 in gastric cancer cells than that of single AMOs (P = 0.014, 0.024; 0.038, respectively). Migration activity was also clearly decreased as compared to those in randomized and blank control groups (28 ± 4 Vs 54 ± 3, P <0.01; 28 ± 4 Vs 59 ± 4, P < 0.01).
  
  
  CONCLUSIONS
  MTg-AMOs can specifically inhibit the expression of multiple miRNAs, and effectively antagonize proliferation and migration of gastric cancer cells promoted by oncomirs.
authors.affiliation: Department of Gastroenterology, Tenth People's Hospital of Tongji University, Shanghai, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Xu
authors.forename: Wei-Qi
authors.initials: WQ
authors.lastname: Dai
authors.forename: Xuan-Fu
authors.initials: XF
authors.lastname: Xu
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: He
authors.forename: Chuan-Yong
authors.initials: CY
authors.lastname: Guo
mesh_terms: D048708:Cell Growth Processes / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors* / Q000235:genetics*; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics* / Q000494:pharmacology*; D012844:Sincalide / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000628:therapy*; D014162:Transfection / Q000379:methods
chemical_list: D035683:MicroRNAs; D020319:Oligodeoxyribonucleotides, Antisense; D012844:Sincalide