title: Effect of ginseng polysaccharide on the urinary excretion of type 2 diabetic rats studied by liquid chromatography-mass spectrometry.
abstract: Ginseng polysaccharide is known to have anti-hyperglycemic and anti-hyperlipidemic effects in vivo and its precise mechanism of action is not clear. A urinary metabolomics method based on rapid-resolution liquid chromatography/mass spectrometry (RRLC/MS) was developed to investigate the effect of water-soluble ginseng polysaccharide (WGP) on type 2 diabetes in rats. Principal component analysis (PCA) was carried out for pattern recognition and a clear separation between type 2 diabetic rats and those treated with WGP was achieved. Eight potential biomarkers were found and identified. Significantly increased inosine, serotonin, phenylpropionylglycine and dodecanedioic acid showed the effect of WGP on purine metabolism, tryptophan metabolism, fatty acid metabolism and energy metabolism. 1-Methyladenine, 4-deoxyerythronic acid, 5-hydroxyhexanoic acid and tetrahydrocortisol were significantly decreased which indicated that WGP can regulate DNA metabolism, organic acids metabolism and steroid hormone metabolism. This work is helpful in the effect mechanism study of ginseng polysaccharide.
authors.affiliation: Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Niu
authors.forename: Zifeng
authors.initials: Z
authors.lastname: Pi
authors.forename: Hao
authors.initials: H
authors.lastname: Yue
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Yu
authors.forename: Shuying
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D056148:Chromatography, Reverse-Phase / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000652:urine*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics; D005894:Panax / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014556:Urine / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides