title: Structural features of human histone acetyltransferase p300 and its complex with p53.
abstract: The protein p300 is a multifunctional transcriptional coactivator that plays pivotal role in several cellular functions. Although structures of several domains have been solved in isolation, the structures of full-length protein (p300 FL) or its complexes with transcription activators are completely unknown. Herein, we applied atomic force microscopy to visualize p300 FL. We found that it is almost prolate ellipsoidal in shape, having several bulges. We further identified the functionally significant N-terminal and C-terminal regions, by applying domain-specific antibodies and found that they are located near one end and centre of the molecule, respectively. Importantly, we have visualized the complex between p300 FL and tumor suppressor protein p53. The relevance of these data in understanding dynamics of p300 during acetylation and transcription will be mentioned.
authors.affiliation: Department of Biological Chemistry, Indian Association for the Cultivation of Science, Kolkata 700032, India.
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Banerjee
authors.forename: M
authors.initials: M
authors.lastname: Arif
authors.forename: Tatini
authors.initials: T
authors.lastname: Rakshit
authors.forename: Neeladri S
authors.initials: NS
authors.lastname: Roy
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Kundu
authors.forename: Siddhartha
authors.initials: S
authors.lastname: Roy
authors.forename: Rupa
authors.initials: R
authors.lastname: Mukhopadhyay
mesh_terms: D000107:Acetylation; D000911:Antibodies, Monoclonal / Q000737:chemistry; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018625:Microscopy, Atomic Force; D016297:Mutagenesis, Site-Directed; D011485:Protein Binding; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D012996:Solutions; D015533:Transcriptional Activation; D016159:Tumor Suppressor Protein p53 / Q000737:chemistry* / Q000235:genetics; D050880:p300-CBP Transcription Factors / Q000737:chemistry* / Q000235:genetics; D000099224:p300-CBP-Associated Factor
chemical_list: D000911:Antibodies, Monoclonal; D006657:Histones; D011993:Recombinant Fusion Proteins; D012996:Solutions; D016159:Tumor Suppressor Protein p53; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor