title: [Microbial transformation of buflomedil by Cunninghamella blakesleana AS 3.153].
abstract: The microbial transformation of buflomedil by Cunninghamella blakesleana AS 3.153 was studied, as well as a microbial model which can be used to mimic metabolism of buflomedil in mammal was established. Experiments were conducted to screen the capabilities of four strains of Cunninghamella species to transform buflomedil, in which C. blakesleana AS 3.153 was selected for a preparative biotransformation. Furthermore, the microbial model was established based on the transformation condition optimization. The parent drug and its metabolites produced by C. blakesleana AS 3.153 were detected by liquid chromatography-mass spectrometry method and three metabolites were identified while two of them were new found metabolites. Two major metabolites, para-O-desmethyl buflomedil and 12-C-oxidated buflomedil, were isolated by semi-preparative HPLC. Based on the comparison between different species, the microbial transformation of buflomedil by C. blakesleana AS 3.153 is more similar to the metabolism of buflomedil in human and Beagle dog than that in rat.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Yang
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Ma
authors.forename: Ping
authors.initials: P
authors.lastname: Bu
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
mesh_terms: D000328:Adult; D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D020093:Cunninghamella / Q000378:metabolism*; D004285:Dogs; D005260:Female; D006801:Humans; D008297:Male; D015394:Molecular Structure; D011759:Pyrrolidines / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization; D055815:Young Adult
chemical_list: D011759:Pyrrolidines; C010651:buflomedil