title: Polymorphisms in Factor II and Factor V thrombophilia genes among Circassians in Jordan.
abstract: Thrombosis is a major cause of morbidity and mortality worldwide. Genetic factors are one component of thrombosis. We studied the prevalence of two mutations that are known risk factors in the pathogenesis of arterial and venous thrombosis in the genetically isolated Circassian population in Jordan. Factor II G20210A and Factor V Leiden single nucleotide polymorphisms were analysed by polymerase chain reaction and restriction fragment length polymorphism method in 104 random unrelated subjects from the Circassian population in Jordan. The prevalence rates among the Circassian population in Jordan for Factor II G20210A was 12.2% and for Factor V Leiden was 7.7%. We have shown that the population is in Hardy-Weinberg equilibrium and that the prevalences of both mutations are within the range of other ethnic groups. This is the first study to describe Circassian health related genetic characteristics in Jordan. Such population-based studies will contribute to understanding the interaction between genetic and environmental risk factors. It will remain to be seen whether carriers of Factor II G20210A and Factor V Leiden are more likely to develop thrombosis. This issue should be studied in the future to determine the need for screening of these mutations particularly in thrombophilia patients.
authors.affiliation: Department of Biology and Biotechnology, Hashemite University, Zarqa, Jordan. rdajani@hu.edu.jo
authors.forename: R
authors.initials: R
authors.lastname: Dajani
authors.forename: A
authors.initials: A
authors.lastname: Arafat
authors.forename: N
authors.initials: N
authors.lastname: Hakooz
authors.forename: Z
authors.initials: Z
authors.lastname: Al-Abbadi
authors.forename: Al-Motassem
authors.initials: AM
authors.lastname: Yousef
authors.forename: M
authors.initials: M
authors.lastname: El Khateeb
authors.forename: F
authors.initials: F
authors.lastname: Quadan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D019943:Amino Acid Substitution; D005165:Factor V / Q000235:genetics*; D005260:Female; D006801:Humans; D007597:Jordan / Q000453:epidemiology / Q000208:ethnology; D008297:Male; D008875:Middle Aged; D020125:Mutation, Missense; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011516:Prothrombin / Q000235:genetics*; D019851:Thrombophilia / Q000208:ethnology / Q000235:genetics* / Q000401:mortality; D013927:Thrombosis / Q000208:ethnology / Q000235:genetics* / Q000401:mortality
chemical_list: C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin