title: [Bacteriolytic enzymes of blood plasma from sheep].
abstract: In the present work the studies ofbacteriolytic factors from sheep blood plasma have been performed. Three novel enzymes have been identified and characterized. Two of them have a molecular weight 15 +/- 2 kDa and able to lyse the gram-negative Escherichia coli bacteria. The third enzyme has a molecular weight 34 +/- 4 kDa and is able to lyse both gram-negative Escherichia coli and gram-positive Micrococcus luteus bacteria. The bacteriolytic reactions have been studied for all three enzymes; particularly, pH-optima have been identified with respect to the substrate. To identify the enzymes trypsinolysis and consequent MALDI-TOF mass spectrometry studies were performed. The results were compared to data from publicly available databases, such as Swiss-Prot, NCBI, MSDB.
authors.forename: S A
authors.initials: SA
authors.lastname: Sedov
authors.forename: N G
authors.initials: NG
authors.lastname: Belogurova
authors.forename: S V
authors.initials: SV
authors.lastname: Shipovskov
authors.forename: M V
authors.initials: MV
authors.lastname: Semenova
authors.forename: M M
authors.initials: MM
authors.lastname: Gitinov
authors.forename: A V
authors.initials: AV
authors.lastname: Levashov
authors.forename: P A
authors.initials: PA
authors.lastname: Levashov
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D001433:Bacteriolysis; D002473:Cell Wall / Q000737:chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D016208:Databases, Factual; D004926:Escherichia coli / Q000187:drug effects; D016982:Micrococcus luteus / Q000187:drug effects; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D010949:Plasma / Q000201:enzymology*; D012756:Sheep / Q000097:blood*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000900:Anti-Bacterial Agents; D009113:Muramidase; D010447:Peptide Hydrolases