title: Gastroprotective effect and structure of a rhamnogalacturonan from Acmella oleracea.
abstract: The plant Acmella oleracea (L.) R.K.Jansen (Asteraceae), locally known as jambu, is widely used in Legal Amazon in local dishes and in folk medicine. A polysaccharide (SC) was isolated from this plant, following aqueous extraction, which contained uronic acid, galactose, arabinose, rhamnose, and glucose in a 15:2:1:1:0.5 molar ratio and had a M(w) 226,000 g/mol. Methylation analysis and NMR spectroscopy indicated that SC is a rhamnogalacturonan composed of a long chain of →4)-6-OMe-α-D-GalpA-(1→, interspersed with some α-L-Rhap residues, partly substituted by side-chains of type II arabinogalactans. SC significantly inhibited ethanol-induced gastric ulcers in rats with an ED₅₀ of 1.5 mg/kg, indicating that SC acts as gastroprotective agent.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81.531-980, CP 19046, Curitiba, PR, Brazil.
authors.forename: Adamara M
authors.initials: AM
authors.lastname: Nascimento
authors.forename: Lauro M
authors.initials: LM
authors.lastname: de Souza
authors.forename: Cristiane H
authors.initials: CH
authors.lastname: Baggio
authors.forename: Maria Fernanda de P
authors.initials: MF
authors.lastname: Werner
authors.forename: Daniele
authors.initials: D
authors.lastname: Maria-Ferreira
authors.forename: Luisa M
authors.initials: LM
authors.lastname: da Silva
authors.forename: Guilherme L
authors.initials: GL
authors.lastname: Sassaki
authors.forename: Philip A J
authors.initials: PA
authors.lastname: Gorin
authors.forename: Marcello
authors.initials: M
authors.lastname: Iacomini
authors.forename: Thales R
authors.initials: TR
authors.lastname: Cipriani
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000737:chemistry* / Q000627:therapeutic use*; D019659:Asteraceae / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D010368:Pectins; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*
chemical_list: D000897:Anti-Ulcer Agents; D010368:Pectins