title: Expression of Egr1 and p53 in human carotid plaques and apoptosis induced by 7-oxysterol or p53.
abstract: Egr-1 and p53 are involved in pathology of both atherosclerosis and cancer. However, it is unknown whether p53 and Egr1 are interactively involved in apoptosis in atherosclerosis. We found that in human carotid plaques, the expression of p53 was inversely correlated with Egr1. In U937 cells, 7β-hydroxycholesterol and 7-ketocholesterol induced production of reactive oxygen species (ROS), transient up-regulation of Egr1 followed by late induction of p53 and apoptosis. Cells with nuclear fragmentation induced by 7-oxysterol or p53 showed increased levels of p53, but decreased levels of Egr1. In conclusion, ROS induced by 7-oxysterols may function as an early initiator of Egr1 expression. The late induced p53 by 7-oxysterols contributes to apoptotic cell death and is linked to the reduction of Egr1 levels, which resembles the differential expression of p53 and Egr1 in human atheroma progression.
authors.affiliation: Division of Experimental Pathology, Department of Clinical and Experimental Medicine, Linköping University, Linköping SE-581 85, Sweden.
authors.forename: Sayem
authors.initials: S
authors.lastname: Miah
authors.forename: Shahram Nour Mohammad
authors.initials: SN
authors.lastname: Zadeh
authors.forename: Xi-Ming
authors.initials: XM
authors.lastname: Yuan
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016893:Carotid Stenosis / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D018929:Cell Culture Techniques; D051766:Early Growth Response Protein 1 / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D006888:Hydroxycholesterols / Q000633:toxicity*; D007150:Immunohistochemistry; D007653:Ketocholesterols / Q000633:toxicity*; D051379:Mice; D017382:Reactive Oxygen Species / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics; D020298:U937 Cells
chemical_list: C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D006888:Hydroxycholesterols; D007653:Ketocholesterols; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; C011724:cholest-5-en-3 beta,7 alpha-diol; C003001:7-ketocholesterol