title: The genotypes of citrus tristeza virus isolates from China revealed by sequence analysis of multiple molecular markers.
abstract: The genotypes of ten citrus tristeza virus (CTV) isolates from central China were determined by examining multiple molecular markers (MMMs) using 11 primer pairs. The results revealed that one isolate contained a single T30 genotype, two isolates contained a single VT genotype, and the other seven isolates were mixtures of two or more genotypes. Sequence analysis of amplified MMMs showed a high genetic diversity in Chinese CTV populations. The genotypes resembling T36, RB and B165 were identified from Chinese CTV isolates for the first time. Our results suggest that genotype assignment of CTV cannot be based solely on the amplification profiles of MMMs, and sequencing of MMMs is required.
authors.affiliation: National Key Laboratory of Agromicrobiology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
authors.forename: Guan-Wei
authors.initials: GW
authors.lastname: Wu
authors.forename: Song
authors.initials: S
authors.lastname: Pan
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Ni
authors.initials: N
authors.lastname: Hong
mesh_terms: D002681:China; D002957:Citrus / Q000821:virology*; D017790:Closterovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D005819:Genetic Markers