title: Serotypes and virulotypes of non-O157 shiga-toxin producing Escherichia coli (STEC) on bovine hides and carcasses.
abstract: Four hundred and fifty beef animal hides and a similar number of carcasses were screened for STEC in 3 beef abattoirs over a 12 month period using PCR and culture based methods. 67% (301/450) of hides and 27% (122/450) of carcasses were STEC PCR positive. Forty isolates representing 12 STEC serotypes (O5:H-, O13:H2, O26:H11, O33:H11, O55:H11, O113:H4, O128:H8, O136:H12, O138:H48, O150:H2, O168:H8 and ONT:H11) and 15 serotype-virulotype combinations were identified. This study provides much needed non-O157 STEC surveillance data and also provides further evidence of bovines as a source of clinically significant STEC as well as identifying 3 emerging serotypes O5:H- (eae-β1), O13:H2 (eae-ζ), and O150:H2 (eae-ζ) that should be considered when developing beef testing procedures for non-O157 STEC.
authors.affiliation: Department of Food Safety, Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland.
authors.forename: Aine
authors.initials: A
authors.lastname: Monaghan
authors.forename: Brian
authors.initials: B
authors.lastname: Byrne
authors.forename: Séamus
authors.initials: S
authors.lastname: Fanning
authors.forename: Torres
authors.initials: T
authors.lastname: Sweeney
authors.forename: David
authors.initials: D
authors.lastname: McDowell
authors.forename: Declan J
authors.initials: DJ
authors.lastname: Bolton
mesh_terms: D000003:Abattoirs; D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012703:Serotyping; D022603:Shiga Toxins / Q000235:genetics / Q000378:metabolism; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D018829:Adhesins, Bacterial; D029968:Escherichia coli Proteins; D022603:Shiga Toxins; D037521:Virulence Factors