title: Thermoreversible gelation polymer as an embolic material for aneurysm treatment: a delivery device for dermal fibroblasts and basic fibroblast growing factor into experimental aneurysms in rats.
abstract: BACKGROUND
  This study evaluates whether thermoreversible gelation polymer (TGP) can be used as a delivery device to deploy dermal fibroblasts and cytokines into experimental aneurysms in rats.
  
  
  METHODS
  The right common iliac artery of rats was surgically ligated and an experimental aneurysm was created by applying exogenous elastase. Seven days later, two aneurysms were harvested and used as controls (Group A), two were embolized with pure TGP (Group B), two were embolized with TGP and basic fibroblast growth factor (bFGF) (Group C) and two were embolized with TGP loaded with rat dermal fibroblasts (Group D). The aneurysms were also embolized with TGP mixed with dermal fibroblasts and bFGF at different concentrations (10 ng/ml: Group E (n=2), 100 ng/ml: Group F (n=2), 1000 ng/ml: Group G (n=2)). Each aneurysm sample was harvested after 7 days and histologic analyses were performed.
  
  
  RESULTS
  The most advanced thrombus organization in the aneurysm, such as prominent fibroblast proliferation and collagen deposition, was observed in Groups E, F and G, although there was no noticeable difference between the groups. Moderate thrombus organization was seen in Group D and minimal thrombus organization was seen in Groups B and C.
  
  
  CONCLUSIONS
  TGP mixed with both dermal fibroblasts and bFGF induced the most advanced thrombus organization in the experimental aneurysms followed by TGP mixed only with dermal fibroblasts. TGP may be useful as a delivery device to deploy fibroblasts and cytokines into aneurysms.
authors.affiliation: Department of Neurosurgery, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Dobashi
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Akasaki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Yuki
authors.forename: Takao
authors.initials: T
authors.lastname: Arai
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ohashi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Murayama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Takao
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Abe
mesh_terms: D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004396:Coloring Agents; D004621:Embolization, Therapeutic / Q000379:methods*; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000627:therapeutic use*; D005347:Fibroblasts / Q000502:physiology*; D002532:Intracranial Aneurysm / Q000473:pathology / Q000628:therapy*; D008297:Male; D011108:Polymers; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000166:cytology; D013927:Thrombosis / Q000188:drug therapy
chemical_list: D004396:Coloring Agents; D011108:Polymers; D016222:Fibroblast Growth Factor 2; D003094:Collagen