title: Construction of the intermediate  vector pVBG2307 by incorporating vital elements of expression vectors pBI121 and pBI221.
abstract: Molecular  chaperones of plasmid pBI121 carrying CaMV35S promoter and a nucleotide  sequence of plasmid pBI221 were inserted into plasmid pCAMBIA2300 to construct  an intermediate vector: pVBG2307. This novel vector pVBG2307 contains a greatly  expanded multiple cloning site with an adjacent imported CaMV35S promoter  sequence. This vector allows controlled transformation of DNA in both Escherichia  coli and Agrobacterium tumefaciens. Cloned PG, orf456, ipt genes and E8, a fruiting promoter, were amplified by PCR of  cDNA libraries of Capsicum annum and Lycopersicon esculentum and  were then transferred into vector pVBG2307. The viability of this vector was  demonstrated, as it regulated PG, orf456, ipt and E8 genes  in E. coli and could be transferred into Agrobacterium strain  EHA105-4.
authors.affiliation: College of Horticulture, Northwest A&F University, Shaanxi, Yangling, P.R. China.
authors.forename: S S
authors.initials: SS
authors.lastname: Ahmed
authors.forename: Z-H
authors.initials: ZH
authors.lastname: Gong
authors.forename: J-J
authors.initials: JJ
authors.lastname: Ji
authors.forename: Y-X
authors.initials: YX
authors.lastname: Yin
authors.forename: H-J
authors.initials: HJ
authors.lastname: Xiao
authors.forename: M A
authors.initials: MA
authors.lastname: Khan
authors.forename: A
authors.initials: A
authors.lastname: Rehman
authors.forename: I
authors.initials: I
authors.lastname: Ahmad
mesh_terms: D002212:Capsicum / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D017343:Genes, Plant / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011096:Polygalacturonase / Q000235:genetics; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D015203:Reproducibility of Results
chemical_list: D004262:DNA Restriction Enzymes; D011096:Polygalacturonase