title: Prevalence and Risk Factors of Toxoplasma gondii Infection in Domestic Cats from the Tropics of Mexico Using Serological and Molecular Tests.
abstract: The aim of this study was to determine the prevalence and risk factors associated with Toxoplasma gondii infection in domestic cats using an indirect-ELISA (IgM and IgG) and PCR. Samples collected from 220 cats from Merida, Yucatan, Mexico, were analyzed. Cases were reported as acute or chronic. Cases when positive to IgM and IgG and PCR were considered as reactivated chronic infection. Risk factors (sex, age, body condition, diet access to hunting, and number of cats in home) were assessed with a multivariate analysis, 75.5% (166/220) of the cats were IgM and 91.8% (202/220) IgG-seropositive and 79% were PCR-positive (173/220). Number of cats per household and low body condition score were associated with reactivated chronic infection (P < 0.05). It is concluded that T. gondii is scattered in the studied population with several periods of reinfection, and therefore an environmental contamination with infecting oocysts exists and there are intrinsic associated factors in cats that increase the risk of becoming infected.
authors.affiliation: Departamento de Salud Animal y Medicina Preventiva, CA Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autonoma de Yucatán, Km. 15.5 Carretera, Merida-Xmatkuil, Apd. 4-116, Merida, YUC, Mexico.
authors.forename: Virgen J
authors.initials: VJ
authors.lastname: Castillo-Morales
authors.forename: Karla Y
authors.initials: KY
authors.lastname: Acosta Viana
authors.forename: Eugenia Del S
authors.initials: Edel S
authors.lastname: Guzmán-Marín
authors.forename: Matilde
authors.initials: M
authors.lastname: Jiménez-Coello
authors.forename: José C
authors.initials: JC
authors.lastname: Segura-Correa
authors.forename: A J
authors.initials: AJ
authors.lastname: Aguilar-Caballero
authors.forename: Antonio
authors.initials: A
authors.lastname: Ortega-Pacheco