title: miR-200c enhances radiosensitivity of human breast cancer cells.
abstract: Due to the intrinsic resistance of many tumors to radiotherapy, current methods to improve the survival of cancer patients largely depend on increasing tumor radiosensitivity. It is well-known that miR-200c inhibits epithelial-mesenchymal transition (EMT), and enhances cancer cell chemosensitivity. We sought to clarify the effects of miR-200c on the radiosensitization of human breast cancer cells. In this study, we found that low levels of miR-200c expression correlated with radiotolerance in breast cancer cells. miR-200c overexpression could increase radiosensitivity in breast cancer cells by inhibiting cell proliferation, and by increasing apoptosis and DNA double-strand breaks. Additionally, we found that miR-200c directly targeted TANK-binding kinase 1 (TBK1). However, overexpression of TBK1 partially rescued miR-200c mediated apoptosis induced by ionizing radiation. In summary, miR-200c can be a potential target for enhancing the effect of radiation treatment on breast cancer cells.
authors.affiliation: Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Xiangyin Road, Shanghai 200433, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
authors.forename: Fu
authors.initials: F
authors.lastname: Gao
authors.forename: R E J
authors.initials: RE
authors.lastname: Mitchel
authors.forename: Luqian
authors.initials: L
authors.lastname: Zhao
authors.forename: Yanyong
authors.initials: Y
authors.lastname: Yang
authors.forename: Jixiao
authors.initials: J
authors.lastname: Lei
authors.forename: Jianming
authors.initials: J
authors.lastname: Cai
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D053903:DNA Breaks, Double-Stranded; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D011836:Radiation Tolerance / Q000235:genetics*; D011838:Radiation-Sensitizing Agents
chemical_list: C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D011838:Radiation-Sensitizing Agents; D017346:Protein Serine-Threonine Kinases; C403191:TBK1 protein, human