title: Long-term preservation of chilled canine semen using vitamin C in combination with green tea polyphenol.
abstract: Vitamin C and green tea polyphenol are known to have antioxidant effects. The aim of this study was to evaluate the quality of canine semen after preservation with diluents containing vitamin C and polyphenol at 5 degree C for 4 weeks. In experiment 1, we investigated the effects of vitamin C combined with polyphenol supplementation on chilled semen quality. The addition of vitamin C (0.5 or 1 mM) with 0.75 mg per mL polyphenol to semen extender provided significantly higher percentages of sperm motility and viability during cold storage compared to unsupplemented semen. In experiment 2, we determined the optimal working concentration of vitamin C in the semen extender by comparison of a range of concentrations between 0.1 and 20 mM. Supplementation of 0.5 mM vitamin C plus polyphenol yielded the highest percentages of sperm motility and viability; however, there was no beneficial effect on the plasma membrane and acrosomal integrity of the spermatozoa.
authors.affiliation: Laboratory of Animal Reproduction, Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Manita
authors.initials: M
authors.lastname: Wittayarat
authors.forename: Taichi
authors.initials: T
authors.lastname: Kimura
authors.forename: Risa
authors.initials: R
authors.lastname: Kodama
authors.forename: Zhao
authors.initials: Z
authors.lastname: Namula
authors.forename: Kaywalee
authors.initials: K
authors.lastname: Chatdarong
authors.forename: Mongkol
authors.initials: M
authors.lastname: Techakumphu
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sato
authors.forename: Masayasu
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Takeshige
authors.initials: T
authors.lastname: Otoi
mesh_terms: D000177:Acrosome / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism*; D002392:Catechin / Q000378:metabolism; D002470:Cell Survival; D003451:Cryoprotective Agents / Q000378:metabolism; D004285:Dogs; D008297:Male; D059808:Polyphenols / Q000378:metabolism*; D012661:Semen; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism; D013662:Tea / Q000737:chemistry
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D059808:Polyphenols; D013662:Tea; D002392:Catechin; D001205:Ascorbic Acid