title: Pineapple stem bromelain immobilized on different supports: catalytic properties in model wine.
abstract: Bromelain from pineapple stem has been covalently immobilized on different supports to select the more efficient biocatalyst that should be applied toward unstable proteins in real white wine. In this preliminary study, catalytic properties of different immobilized bromelain forms were compared under wine-like conditions, against a synthetic substrate (Bz-Phe-Val-Arg-pNA).Covalent immobilization affected protease kinetic properties, even if all immobilized forms presented both a better substrate affinity and higher half-life (with the exception of a few procedures) with respect to the free enzyme. Stem bromelain was successfully immobilized on chitosan beads without glutaraldehyde thus yielding a food-safe and promising biocatalyst for unstable real wine future application.
authors.affiliation: Dept. for Innovation in Biological, Agro-food and Forest systems (DIBAF), Tuscia University, via S. Camillo de Lellis, Viterbo, Italy. ilaria.be@tiscali.it
authors.forename: Benucci
authors.initials: B
authors.lastname: Ilaria
authors.forename: Esti
authors.initials: E
authors.lastname: Marco
authors.forename: Liburdi
authors.initials: L
authors.lastname: Katia
authors.forename: Garzillo Anna
authors.initials: GA
authors.lastname: Maria Vittoria
mesh_terms: D031230:Ananas / Q000201:enzymology*; D019149:Bioreactors; D001963:Bromelains / Q000737:chemistry / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008956:Models, Chemical; D009842:Oligopeptides / Q000737:chemistry; D014920:Wine
chemical_list: D004800:Enzymes, Immobilized; D009842:Oligopeptides; D001963:Bromelains; D048271:Chitosan; C091760:stem bromelain