title: Comparative diffusivity measurements for alginate-based atomized and inkjet-bioprinted artificial cells using fluorescence microscopy.
abstract: Radial diffusivity profiles of atomized (MC, d = 1800 ± 200 µm) and inkjet-printed (MI, d = 40 ± 5 µm) alginate-based artificial cells have been generated using 2D Fluorescence Microscopy. The passive outward diffusion of FITC-Dextrans from MIs (0.5% LV alginate/15% CaCl2 coated with 0.5% Chitosan) and MCs (1.5% MV alginate/1.5% CaCl2) was measured and quantified using a Fickian model. As an expected outcome of miniaturization, the ratios of the outer layer diffusivities defined as D(MIout)/D(MCout) were 4.25 and 5.07 respectively for the 4 and 70 kDa markers, indicative of the enhanced diffusive potential of the miniaturized capsules.
authors.affiliation: Department of Biomedical, Chemical and Materials Engineering, Davidson College of Engineering, San Jose State University, San Jose, CA 95192, USA. maryam.mobed-miremadi@sjsu.edu
authors.forename: Maryam
authors.initials: M
authors.lastname: Mobed-Miremadi
authors.forename: Behrokh
authors.initials: B
authors.lastname: Asi
authors.forename: Jaino
authors.initials: J
authors.lastname: Parasseril
authors.forename: Emily
authors.initials: E
authors.lastname: Wong
authors.forename: Man
authors.initials: M
authors.lastname: Tat
authors.forename: Yiming
authors.initials: Y
authors.lastname: Shan
mesh_terms: D000464:Alginates / Q000737:chemistry*; D058045:Artificial Cells / Q000737:chemistry*; D062028:Bioprinting / Q000379:methods*; D002122:Calcium Chloride / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003911:Dextrans; D004058:Diffusion; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D005456:Fluorescent Dyes; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels; D007700:Kinetics; D008856:Microscopy, Fluorescence; D010316:Particle Size
chemical_list: D000464:Alginates; D003911:Dextrans; D005456:Fluorescent Dyes; D006603:Hexuronic Acids; D020100:Hydrogels; C015219:fluorescein isothiocyanate dextran; D020723:Glucuronic Acid; D048271:Chitosan; D016650:Fluorescein-5-isothiocyanate; D002122:Calcium Chloride