title: Microbiological aspects and shelf life of processed seafood products.
abstract: BACKGROUND
  Fresh fish and seafoods are very perishable products mainly owing to microbial activity of specific spoilage micro-organisms. Application of hurdle technology leads to a variety of processed products with extended shelf life. In this study, sensory evaluation and microbiological analysis were carried out on 17 processed seafood products stored at 4 °C to determine their shelf life and the predominant spoilage micro-organisms.
  
  
  RESULTS
  Shelf life determined by sensory analysis varied from 66 to 180 days depending on the product. The cause of spoilage for most of the products was the development of off-flavours/off-odours, while two products were rejected owing to oil discolouration. Pseudomonads were in most cases below detection limit. H₂S-producing bacteria, Brochothrix thermosphacta and Enterobacteriaceae were below detection limit throughout the experiment. The predominant spoilage micro-organisms were lactic acid bacteria and yeasts. Hygiene indicators such as Staphylococcus spp. and total coliforms were also below detection limit in all samples.
  
  
  CONCLUSIONS
  Primarily the initial pH and secondarily the NaCl content determined shelf life duration. Under the applied conditions, lactic acid bacteria and yeasts predominated. The contribution of chemical oxidation and/or autolysis to spoilage and shelf life might be important for most of the products.
authors.affiliation: Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fitokou Street, GR-38446 Nea Ionia, Volos, Greece. boziaris@uth.gr
authors.forename: Ioannis S
authors.initials: IS
authors.lastname: Boziaris
authors.forename: Anastasios P
authors.initials: AP
authors.lastname: Stamatiou
authors.forename: George-John E
authors.initials: GJ
authors.lastname: Nychas
mesh_terms: D000818:Animals; D003116:Color; D004032:Diet / Q000208:ethnology; D005396:Fish Products / Q000032:analysis / Q000382:microbiology; D005399:Fishes; D063427:Food Quality; D061353:Food Storage; D057141:Food, Preserved / Q000032:analysis / Q000382:microbiology*; D006115:Greece; D006801:Humans; D006863:Hydrogen-Ion Concentration; D056546:Lactobacillales / Q000254:growth & development / Q000302:isolation & purification; D050296:Microbial Viability; D049831:Octopodiformes; D009812:Odorants; D017747:Seafood / Q000032:analysis* / Q000382:microbiology*; D012677:Sensation; D012758:Shellfish / Q000032:analysis / Q000382:microbiology; D017673:Sodium Chloride, Dietary / Q000032:analysis; D015003:Yeasts / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D017673:Sodium Chloride, Dietary