title: Elasticity maps of living neurons measured by combined fluorescence and atomic force microscopy.
abstract: Detailed knowledge of mechanical parameters such as cell elasticity, stiffness of the growth substrate, or traction stresses generated during axonal extensions is essential for understanding the mechanisms that control neuronal growth. Here, we combine atomic force microscopy-based force spectroscopy with fluorescence microscopy to produce systematic, high-resolution elasticity maps for three different types of live neuronal cells: cortical (embryonic rat), embryonic chick dorsal root ganglion, and P-19 (mouse embryonic carcinoma stem cells) neurons. We measure how the stiffness of neurons changes both during neurite outgrowth and upon disruption of microtubules of the cell. We find reversible local stiffening of the cell during growth, and show that the increase in local elastic modulus is primarily due to the formation of microtubules. We also report that cortical and P-19 neurons have similar elasticity maps, with elastic moduli in the range 0.1-2 kPa, with typical average values of 0.4 kPa (P-19) and 0.2 kPa (cortical). In contrast, dorsal root ganglion neurons are stiffer than P-19 and cortical cells, yielding elastic moduli in the range 0.1-8 kPa, with typical average values of 0.9 kPa. Finally, we report no measurable influence of substrate protein coating on cell body elasticity for the three types of neurons.
authors.affiliation: Department of Physics and Astronomy, Tufts University, Medford, Massachusetts, USA.
authors.forename: Elise
authors.initials: E
authors.lastname: Spedden
authors.forename: James D
authors.initials: JD
authors.lastname: White
authors.forename: Elena N
authors.initials: EN
authors.lastname: Naumova
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.forename: Cristian
authors.initials: C
authors.lastname: Staii
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002540:Cerebral Cortex / Q000166:cytology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004548:Elasticity / Q000187:drug effects; D053595:Embryonic Stem Cells / Q000166:cytology; D005727:Ganglia, Spinal / Q000166:cytology; D051379:Mice; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D015739:Nocodazole / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology; D051381:Rats; D013499:Surface Properties; D013997:Time Factors
chemical_list: D017239:Paclitaxel; D015739:Nocodazole