title: Association of genetic polymorphisms in IL17A and IL17F with gastro-duodenal diseases.
abstract: OBJECTIVE
  The important role of IL-17 in inflammatory response to Helicobacter pylori (H. pylori) colonization has been indicated. We investigated the associations between gastro-duodenal diseases and polymorphisms of IL17A (rs2275913 G>A) and IL17F (rs763780 T>C).
  
  
  METHODS
  The study was performed in 548 subjects (363 controls and 185 peptic ulcer cases). The multiplex PCR-SSCP method was used to detect gene polymorphisms.
  
  
  RESULTS
  Overall, number of rs2275913 A allele was significantly associated with an increased risk for peptic ulcer (OR, 1.50; 95%CI, 1.11-2.01; p=0.0082). The frequency of rs2275913 GA+AA genotype was also significantly higher in ulcer cases than controls (OR, 1.72; 95%CI, 1.09-2.72; p=0.020). The rs2275913 GA+AA genotype conferred an increased risk for the severity of gastric mucosal atrophy in subjects younger than 60 years (OR, 2.83; 95%CI, 1.14-7.04; p=0.025). Both atrophy and metaplasia were increased with age in rs2275913 GA+AA genotype. In NSAIDs/aspirin users, number of rs2275913 A allele was associated with an increased risk for a peptic ulcer (OR, 3.98; 95%CI, 1.48-10.7; p=0.0061). There was no association of rs763780 with the development of peptic ulcer.
  
  
  CONCLUSIONS
  Our results provide the evidence that rs2275913 is associated with an increased risk for peptic ulcer and the severity of the gastric mucosal atrophy in comparatively younger subjects. In addition, this allele is also associated with the increased risk for peptic ulcer in NSAIDs/aspirin users.
authors.affiliation: Department of Gastroenterology, Kanazawa Medical University, Ishikawa, Japan.
authors.forename: Ranji
authors.initials: R
authors.lastname: Hayashi
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Tahara
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Shiroeda
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Matsue
authors.forename: Takahiro
authors.initials: T
authors.lastname: Minato
authors.forename: Tomoe
authors.initials: T
authors.lastname: Nomura
authors.forename: Hideto
authors.initials: H
authors.lastname: Yamada
authors.forename: Takashi
authors.initials: T
authors.lastname: Saito
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Matsunaga
authors.forename: Tomoki
authors.initials: T
authors.lastname: Fukuyama
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Hayashi
authors.forename: Toshimi
authors.initials: T
authors.lastname: Otsuka
authors.forename: Atsushi
authors.initials: A
authors.lastname: Fukumura
authors.forename: Masakastu
authors.initials: M
authors.lastname: Nakamura
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Tsutsumi
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Tomiyasu
authors.initials: T
authors.lastname: Arisawa
mesh_terms: D000328:Adult; D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects; D016022:Case-Control Studies; D004381:Duodenal Ulcer / Q000235:genetics* / Q000382:microbiology; D005260:Female; D005757:Gastritis, Atrophic / Q000235:genetics* / Q000382:microbiology; D016481:Helicobacter Infections / Q000150:complications; D016480:Helicobacter pylori; D006801:Humans; D020381:Interleukin-17 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013276:Stomach Ulcer / Q000235:genetics* / Q000382:microbiology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; C508717:IL17A protein, human; C440021:IL17F protein, human; D020381:Interleukin-17