title: [Role of Bcl-2 in the regulation of creb activity and vasopressin expression in the hypothalamic neurons of rats].
abstract: The antiapoptotic protein Bcl-2 has various functions besides its role in protecting cells from apoptosis. Previous studies have demonstrated that Bcl-2 recruits ERK1/2 and/or CREB to initiate different transcription program in the regulation of various neuronal activities as well as axonal growth. Recently we reported that Bcl-2 can participate in the regulation of synthesis and secretion of vasopressin of rat hypothalamic magnocellular nuclei. In thise study we have investigated the inhibition of Bcl-2 on vasopressin expression in magnocellular neurons of hypothalamic supraoptic nuclei. The experiments were done on short-term incubated rat hypothalamic slices containing supraoptic nuclei. Our data demonstrated that in vitro inhibition of Bcl-2 by HA14-1 prevented CREB translocation into the cell nuclei and significantly decreased vasopressin mRNA level and enhanced contents of vasopressin protein in magnocellular neurons in supraoptic nucleus. Our results indicate that CREB-dependent vasopressin gene transcription in the hypothalamic magnocellular neurons can be regulated by Bcl-2.
authors.forename: L S
authors.initials: LS
authors.lastname: Nikitina
authors.forename: E V
authors.initials: EV
authors.lastname: Chernigovskaia
authors.forename: N A
authors.initials: NA
authors.lastname: Dorofeeva
authors.forename: O D
authors.initials: OD
authors.lastname: Kirillova
authors.forename: M V
authors.initials: MV
authors.lastname: Glazova
mesh_terms: D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D007031:Hypothalamus / Q000378:metabolism; D007150:Immunohistochemistry; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D009474:Neurons / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D051381:Rats; D013495:Supraoptic Nucleus / Q000187:drug effects; D014667:Vasopressins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
chemical_list: D001578:Benzopyrans; D017362:Cyclic AMP Response Element-Binding Protein; D009570:Nitriles; D019253:Proto-Oncogene Proteins c-bcl-2; C456800:ethyl 2-amino-6-bromo-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate; D014667:Vasopressins