title: [Zoledronic acid incorporated in chitosan/calcium phosphate ceramic: characterization and in vitro response of osteoblast cells].
abstract: OBJECTIVE
  To develop a new local delivery system, zoledronic-acid-loaded chitosan/calcium phosphate ceramic, and to determine its characterization and in vitro response of osteoblast cells.
  
  
  METHODS
  Zoledronic-acid-loaded chitosan/calcium phosphate ceramic were prepared by solution casting method at a concentration of 10(-5), 10(-4), and 10(-3) mol/L, respectively. The physicochemical properties of the resulting materials were determined using SEM and FTIR. Drug absorbance was measured using CCK-8 colorimetric assay and alkaline phosphatase assay to detect the effect of drug-loaded materials on the proliferation and differentiation of osteoblasts.
  
  
  RESULTS
  After ZOL loading, SEM showed that porous calcium phosphate ceramic was coated with chitosan evenly. The IR spectra indicated that drug absorption peaks were shifted and a new one was formed for the drug-loaded biomaterials. The material at the highest concentration could inhibit the proliferation and alkaline phosphatase activities of osteoblast cells, but no such effect was found at a drug-loading concentration of 10(-4)-10(-5) mol/L.
  
  
  CONCLUSIONS
  We confirmed that the local delivery system in this study has ability of loading ZOL. The biomaterial with high drug concentrations inhibits the proliferation and differentiation of osteoblasts, but not when the drug concentrations are low.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Cheng-Cheng
authors.initials: CC
authors.lastname: Zang
authors.forename: Min-Hua
authors.initials: MH
authors.lastname: Teng
authors.forename: Jun-Jiang
authors.initials: JJ
authors.lastname: Zhai
authors.forename: Yun-Mao
authors.initials: YM
authors.lastname: Liao
authors.forename: Jian
authors.initials: J
authors.lastname: Liao
authors.forename: Ning-Yuan
authors.initials: NY
authors.lastname: Sun
authors.forename: Xing
authors.initials: X
authors.lastname: Liang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002130:Calcium Phosphates / Q000008:administration & dosage / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry; D048271:Chitosan / Q000008:administration & dosage / Q000737:chemistry*; D004164:Diphosphonates / Q000494:pharmacology*; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry*; D005260:Female; D007093:Imidazoles / Q000494:pharmacology*; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077211:Zoledronic Acid
chemical_list: D002130:Calcium Phosphates; D004164:Diphosphonates; D004337:Drug Carriers; D007093:Imidazoles; D000077211:Zoledronic Acid; D048271:Chitosan; C020243:calcium phosphate