title: The ABC transporter ABC40 encodes a phenylacetic acid export system in Penicillium chrysogenum.
abstract: The filamentous fungus Penicillium chrysogenum is used for the industrial production of β-lactam antibiotics. The pathway for β-lactam biosynthesis has been resolved and involves the enzyme phenylacetic acid CoA ligase that is responsible for the CoA activation of the side chain precursor phenylacetic acid (PAA) that is used for the biosynthesis of penicillin G. To identify ABC transporters related to β-lactam biosynthesis, we analyzed the expression of all 48 ABC transporters present in the genome of P. chryso-genum when grown in the presence and absence of PAA. ABC40 is significantly upregulated when cells are grown or exposed to high levels of PAA. Although deletion of this transporter did not affect β-lactam biosynthesis, it resulted in a significant increase in sensitivity to PAA and other weak acids. It is concluded that ABC40 is involved in weak acid detoxification in P. chrysogenum including resistance to phenylacetic acid.
authors.affiliation: Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, Zernike Institute for Advanced Materials and Kluyver Centre for Genomics of Industrial Fermentation, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands. s.weber@rug.nl
authors.forename: Stefan S
authors.initials: SS
authors.lastname: Weber
authors.forename: Andriy
authors.initials: A
authors.lastname: Kovalchuk
authors.forename: Roel A L
authors.initials: RA
authors.lastname: Bovenberg
authors.forename: Arnold J M
authors.initials: AJ
authors.lastname: Driessen
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000097:blood* / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D010408:Penicillium chrysogenum / Q000235:genetics / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism*; D015854:Up-Regulation; D047090:beta-Lactams / Q000378:metabolism
chemical_list: D018528:ATP-Binding Cassette Transporters; D005656:Fungal Proteins; D010648:Phenylacetates; D047090:beta-Lactams; C025136:phenylacetic acid