title: The experimental study of hemodynamic changes and pathological morphology after artery anastomosis.
abstract: The purpose of this article is to dynamically observe hemodynamic and medium-term pathomorphological changes and to investigate the pathophysiological variation at the vascular anastomotic stoma. Eighteen New Zealand white rabbits underwent right common carotid artery in situ anastomosis, and the hemodynamic changes of anastomotic stoma were recorded by color Doppler ultrasonography before surgery and 5 minutes, 1 week, 2 weeks, 3 weeks, 4 weeks, 8 weeks, 12 weeks, and 16 weeks after surgery. Pathological sections of stoma were made and the pathomorphological changes viewed. Artery anastomoses were preformed successfully in 15 rabbits; 4 rabbits suffered postoperative death. It took 1.22 ± 0.77 hours to complete every operation. All the data involved, mean velocity, peak systolic velocity, and resistant index, had no significant differences between preoperative and postoperative follow-up. The obvious differences were found in the diameters of stoma 5 minutes, 1 week, 2 weeks, and 3 weeks after operation (P values were .002, .001, .003, and .008, respectively). The variance of stoma diameters 2 weeks later was also different from those after 1 week, but no other differences were observed during other weeks. The decrease in stoma diameters after anastomosis was clear, with the maximum decrease occurring 2 weeks after the operation, after which the diameters of stoma became stable 16 weeks after the operation. Some collagens around anastomoses could be found by the pathological sections of stoma, which indicated that the deposition of collagen might be related to the decrease in stoma diameter.
authors.affiliation: Shanghai Jiaotong University, Shanghai, China.
authors.forename: Junbiao
authors.initials: J
authors.lastname: Hang
authors.forename: Liang
authors.initials: L
authors.lastname: Sui
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.forename: Shuliang
authors.initials: S
authors.lastname: Lu
authors.forename: Fei
authors.initials: F
authors.lastname: Song
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiqiao
authors.initials: X
authors.lastname: Wang
mesh_terms: D000704:Analysis of Variance; D000714:Anastomosis, Surgical / Q000009:adverse effects*; D000818:Animals; D017536:Carotid Artery, Common / Q000000981:diagnostic imaging / Q000473:pathology* / Q000601:surgery; D003094:Collagen / Q000378:metabolism; D023903:Coronary Restenosis; D004195:Disease Models, Animal; D018618:Echocardiography, Doppler, Color; D005109:Extracellular Matrix; D005260:Female; D006439:Hemodynamics; D006801:Humans; D008297:Male; D011817:Rabbits
chemical_list: D003094:Collagen