title: Dynamic localization of Tat protein transport machinery components in Streptomyces coelicolor.
abstract: The Tat pathway transports folded proteins across the bacterial cytoplasmic membrane and is a major route of protein export in the Streptomyces genus of bacteria. In this study, we have examined the localization of Tat components in the model organism Streptomyces coelicolor by constructing enhanced green fluorescent protein (eGFP) and mCherry fusions with the TatA, TatB, and TatC proteins. All three components colocalized dynamically in the vegetative hyphae, with foci of each tagged protein being prominent at the tips of emerging germ tubes and of the vegetative hyphae, suggesting that this may be a primary site of Tat secretion. Time-lapse imaging revealed that localization of the Tat components was highly dynamic during tip growth and again demonstrated a strong preference for apical sites in growing hyphae. During aerial hypha formation, TatA-eGFP and TatB-eGFP fusions relocalized to prespore compartments, indicating repositioning of Tat components during the Streptomyces life cycle.
authors.affiliation: Molecular Biotechnology, Institute of Biology, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands.
authors.forename: Joost
authors.initials: J
authors.lastname: Willemse
authors.forename: Beata
authors.initials: B
authors.lastname: Ruban-Ośmialowska
authors.forename: David
authors.initials: D
authors.lastname: Widdick
authors.forename: Katherine
authors.initials: K
authors.lastname: Celler
authors.forename: Matthew I
authors.initials: MI
authors.lastname: Hutchings
authors.forename: Gilles P
authors.initials: GP
authors.lastname: van Wezel
authors.forename: Tracy
authors.initials: T
authors.lastname: Palmer
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism*; D010957:Plasmids; D021381:Protein Transport / Q000502:physiology*; D011994:Recombinant Proteins; D048372:Streptomyces coelicolor / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D059008:Time-Lapse Imaging
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins