title: [Development and preliminary assessment of a recombinant canarypox virus as an antirabic vaccine candidate].
abstract: Development and preliminary assessment of a recombinant canarypox virus as an antirabic vaccine candidate. In Argentina, rabies is limited to some northern provinces. Availability of new vaccines abolishing the handling of the rabies virus and allowing disease control has regional and national strategic importance. Vaccines based on recombinant poxviruses have been successfully used as antirabic vaccines worldwide. Although these systems are not commercially available, the platform to obtain recombinant canarypox viruses (CNPV) has been previously set up in our laboratory. The aim of this work was the development and evaluation of an antirabic vaccine candidate based on recombinant CNPV expressing the rabies virus (RV) glycoprotein G (RG). A recombinant virus (CNPV-RG) expressing the RG coding sequence was designed. Inoculation of mice with this virus induced high RV seroneutralizing antibodies (3.58 and 9.76 IU/ml after 1 or 2 immunizations, respectively) and protected 78% of intracerebrally RV-challenged animals. In addition, it was determined that CNPV-RG has a relative potency of 3.5 IU/ml. The obtained results constituted the first stage of CNPV-RG evaluation as antirabic vaccine candidate. Further assays will be necessary to confirm its utility in species of veterinary interest.
authors.affiliation: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina. fzanetti@cnia.inta.gov.ar
authors.forename: Flavia
authors.initials: F
authors.lastname: Zanetti
authors.forename: Liliana
authors.initials: L
authors.lastname: Rudak
authors.forename: Matías
authors.initials: M
authors.lastname: Micucci
authors.forename: Daniela Conte
authors.initials: DC
authors.lastname: Grand
authors.forename: Andrea
authors.initials: A
authors.lastname: Luque
authors.forename: Susana
authors.initials: S
authors.lastname: Russo
authors.forename: Oscar
authors.initials: O
authors.lastname: Taboga
authors.forename: Oscar
authors.initials: O
authors.lastname: Pérez
authors.forename: Gabriela
authors.initials: G
authors.lastname: Calamante
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D028121:Canarypox virus / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D002460:Cell Line / Q000821:virology; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D006224:Cricetinae; D005347:Fibroblasts / Q000821:virology; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D007668:Kidney; D008647:Mesocricetus; D051379:Mice; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011818:Rabies / Q000517:prevention & control; D011819:Rabies Vaccines / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D014614:Vaccines, Synthetic / Q000276:immunology; D014709:Vero Cells / Q000821:virology; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014776:Virus Cultivation
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D010446:Peptide Fragments; D011819:Rabies Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus