title: Analysis of polymorphisms in interleukin-10, interleukin-6, and interleukin-1 receptor antagonist in Mexican-Mestizo women with pre-eclampsia.
abstract: Due to the fact that studies seeking associations of polymorphisms in regulatory regions of cytokine genes with pre-eclampsia (PE) have not always been consistent in different population analyses, the aim of this study was to investigate the possible association between rs1800896 of interleukin-10 (IL-10), rs1800795 of interleukin-6 (IL-6), and the variable number of tandem repeats (VNTR) in intron 2 of interleukin-1 receptor antagonist (IL-1Ra), as well as gene-gene interactions between these three polymorphisms with the presence of PE in Mexican-Mestizo women and one Amerindian population from México (Maya). A case-control study was performed where 411 pre-eclamptic cases and 613 controls were genotyped. For the rs1800896 of IL-10 and rs1800795 of IL-6, we used real-time polymerase chain reaction (PCR) allelic discrimination and for the VNTR of IL-1Ra, PCR. Allele frequency differences were assessed by Chi-squared test; logistic regression was used to test for associations; a gene-gene interaction was conducted. Genotypic and allelic distribution of the polymorphisms was similar in our population. The estimated of the gene-gene interaction between the polymorphisms did not differ significantly. However, we observed important differences in the distribution of the alleles and genotypes of the three polymorphisms analyzed between Mestiza-Mexicanas and Maya-Mestizo women. In conclusion, we did not find an association between polymorphisms in IL-10, IL-6, and IL-1Ra and PE in Mexican-Mestizo and Maya-Mestizo women. To our knowledge, this is the first time that these three polymorphisms were analyzed together with gene-gene interaction in women with PE.
authors.affiliation: División de Investigación Biomédica, Subdirección de Enseñanza e Investigación, Centro Médico Nacional 20 de Noviembre, Instituto de Seguridad y Servicios Sociales de los Trabajadores del Estado, México, D.F., México.
authors.forename: Elith Yazmin
authors.initials: EY
authors.lastname: Valencia Villalvazo
authors.forename: Thelma
authors.initials: T
authors.lastname: Canto-Cetina
authors.forename: Juan Fernando
authors.initials: JF
authors.lastname: Romero Arauz
authors.forename: Ramón Mauricio
authors.initials: RM
authors.lastname: Coral-Vázquez
authors.forename: Samuel
authors.initials: S
authors.lastname: Canizales-Quinteros
authors.forename: Agustín
authors.initials: A
authors.lastname: Coronel
authors.forename: Juan
authors.initials: J
authors.lastname: Carlos Falcón
authors.forename: Jaime
authors.initials: J
authors.lastname: Hernández Rivera
authors.forename: Roberto
authors.initials: R
authors.lastname: Ibarra
authors.forename: Lucila
authors.initials: L
authors.lastname: Polanco Reyes
authors.forename: Patricia
authors.initials: P
authors.lastname: Canto
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D007438:Introns; D016015:Logistic Models; D008800:Mexico / Q000208:ethnology; D018598:Minisatellite Repeats; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000208:ethnology* / Q000235:genetics*; D011247:Pregnancy
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D015850:Interleukin-6; D016753:Interleukin-10