title: [Difference of chemical constituents contained in Tibetan herb Jiadiranguo from different habitats by HPLC fingerprint].
abstract: OBJECTIVE
  To establish the HPLC fingerprint for Halenia elliptica herbs, a traditional Tibetan medicine, in order to study constituents contained in H. elliptica from different habitats and compare their differences.
  
  
  METHODS
  HPLC analysis was made on a Welchrom-C18 (4.6 mm x 250 mm, 5 microm) with water and acetonitrile as mobile phase. The wavelength was detected as 265 nm, the flow rate was 1.0 mL x min(-1), and the column temperature was 40 degrees C. The software for chromatographic fingerprint was applied to analyze the similarity. And principal component analysis was conducted.
  
  
  RESULTS
  Twelve common chromatographic peaks were identified by fingerprint, showing a low similarity in constituent and variety. The significant difference in the proportion between xanthones and aglycones in each batch of herbs indicated no notable correlation between constituent characteristics and geographic locations of habitats.
  
  
  CONCLUSIONS
  The method is so simple, exclusive, stable and highly repeatable that it can provide reference for identification and quality assessment of H. elliptica herbs.
authors.affiliation: Ethnomidicine School of Chengdu University of Traditional Chinese Medicine, Chengdu 611131, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Gu
authors.forename: Guoyue
authors.initials: G
authors.lastname: Zhong
authors.forename: Weizao
authors.initials: W
authors.lastname: Luo
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Changhua
authors.initials: C
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: Jifeng
authors.initials: J
authors.lastname: Zhao
authors.forename: Huarong
authors.initials: H
authors.lastname: Zhou
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D017753:Ecosystem; D029965:Gentianaceae / Q000737:chemistry / Q000145:classification
chemical_list: D004365:Drugs, Chinese Herbal