title: Human papillomavirus type 52 polymorphism and high-grade lesions of the uterine cervix.
abstract: The association between polymorphism of human papillomavirus type 52 (HPV52) and high-grade cervical intraepithelial neoplasia (CIN2,3) was investigated in Canadian women. HPV-52-positive endocervical specimens collected from 216 women selected from a total of 3,614 participants recruited in two case-control and two cohort studies conducted in Canada, were further analyzed by PCR-sequencing of the LCR and E6 gene. Overall, the HPV52 LCR prototype was detected more frequently in Caucasian women (69 of 132, 52.3%, 95% confidence interval (CI): 43.8%-60.6%) than in non-Caucasian women (15 of 48, 31.3%, 95% CI 19.9%-45.4%). In two cohort studies, HPV52 prototype was detected in seven of 15 (46.7%, 95% CI 24.8-69.9) HPV52 persistent infections and 14 of 35 (40.0%, 95% CI 25.5-56.5) transient infections (p = 0.76). In two case-control studies, 30 participants did not have CIN, 18 had low-grade CIN (CIN1), 64 had CIN2,3, seven had cervical cancer and the diagnosis was undefined for 27 women. Variant MTL-52-LCR-02 was detected more frequently in women with cancer (28.6%, 95% CI 7.6%-64.8%) than in women without cancer or CIN2,3 (0%, 95% CI 0.0%-9.2%; p = 0.015). CIN2,3 risk was significantly associated with a deletion at nucleotide position 7695 in the LCR (OR 4.9, 95% CI 1.2-20.8), the T7744C variation in the LCR (OR 5.7, 95% CI 1.1-32.0), and the K93R variation in E6 (OR 6.9, 95% CI 1.3-36.8), after adjusting for age, detection of HPV16 or 18 and study site. These findings indicate that HPV52 polymorphism influences risk of CIN-2,3 and possibly invasive cancer.
authors.affiliation: Centre de Recherche et Département de Microbiologie Médicale et Infectiologie, Centre Hospitalier de l'Université de Montréal, Université de Montréal, Québec, Canada.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Formentin
authors.forename: Jacques
authors.initials: J
authors.lastname: Archambault
authors.forename: Anita
authors.initials: A
authors.lastname: Koushik
authors.forename: Harriet
authors.initials: H
authors.lastname: Richardson
authors.forename: Paul
authors.initials: P
authors.lastname: Brassard
authors.forename: Eduardo L
authors.initials: EL
authors.lastname: Franco
authors.forename: Francois
authors.initials: F
authors.lastname: Coutlée
mesh_terms: D000328:Adult; D001483:Base Sequence; D016022:Case-Control Studies; D015331:Cohort Studies; D017931:DNA Primers; D005260:Female; D005814:Genes, Viral; D006801:Humans; D008297:Male; D027383:Papillomaviridae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*
chemical_list: D017931:DNA Primers