title: The development of a loop-mediated isothermal amplification method (LAMP) for Echinococcus granulosus [corrected] coprodetection.
abstract: We have previously developed a polymerase chain reaction (PCR) assay for detection of Echinococcus granulosus infection, which proved very sensitive and specific for identification of infected dogs. We have now developed a loop-mediated isothermal amplification (LAMP) assay, which amplifies the same genomic repeated sequences of E. granulosus for coprodetection. This assay enabled detection of a single egg in fecal samples and showed high species specificity for E. granulosus with no cross-amplification of DNA from closely related helminths, including Echinococcus multilocularis. Because the method does not require thermocycling for DNA amplification, or electrophoresis for amplicon detection, it can potentially be used for premortem identification of E. granulosus-infected dogs to enable large-scale surveys in endemic countries where highly specialized equipment to undertake PCR analysis is rare.
authors.affiliation: Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel. tsvis@ekmd.huji.ac.il
authors.forename: Harold
authors.initials: H
authors.lastname: Salant
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Abbasi
authors.forename: Joseph
authors.initials: J
authors.lastname: Hamburger
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D016054:DNA, Protozoan / Q000032:analysis; D048209:Echinococcus granulosus / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010270:Parasite Egg Count; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan