title: Self-resistance to an antitumor antibiotic: a DNA glycosylase triggers the base-excision repair system in yatakemycin biosynthesis.
abstract: Resistance is (not) futile: The yatakemycin biosynthetic gene cluster involves the ytkR2 gene, which encodes a protein with homology to a recently discovered bacterial DNA glycosylase. Genetic validation in vivo, biochemical assays, and in vitro mutagenesis studies revealed that YtkR2 confers resistance for the bacteria by specifically recognizing and cleaving the YTM-modified base (see scheme).
authors.affiliation: State Key Laboratory of Bioorganic and Natural Products Chemistry, Chinese Academy of Sciences, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Qing-Li
authors.initials: QL
authors.lastname: He
authors.forename: Zhi-Xiong
authors.initials: ZX
authors.lastname: Zhao
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Renxiao
authors.initials: R
authors.lastname: Wang
authors.forename: Jingwu
authors.initials: J
authors.lastname: Kang
authors.forename: Gong-Li
authors.initials: GL
authors.lastname: Tang
mesh_terms: D000595:Amino Acid Sequence; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001409:Bacillus cereus / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D004249:DNA Damage; D045647:DNA Glycosylases / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D019008:Drug Resistance, Neoplasm; D000080890:Duocarmycins; D007211:Indoles / Q000032:analysis / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011758:Pyrroles / Q000032:analysis / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D000080890:Duocarmycins; D007211:Indoles; D011758:Pyrroles; C474180:yatakemycin; D045647:DNA Glycosylases