title: Production and purification of theVP1 capsid protein of a novel canine norovirus using the Saccharomyces cerevisiae expression system.
abstract: The discovery of a novel canine norovirus was recently reported. The VP1 capsid protein of this canine norovirus was expressed in Saccharomyces cerevisiae and purified with a yield of 1.6-1.8 mg/L. The yeast-expressed VP1 protein self-assembled into virus-like particles and exhibited antigenicity, proving to be suitable for serological assays.
authors.affiliation: REQUIMTE, Departamento de Ciências Biológicas, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal. clarafrancisco@gmail.com
authors.forename: Clara
authors.initials: C
authors.lastname: Pereira
authors.forename: Edite
authors.initials: E
authors.lastname: Lopes
authors.forename: João R
authors.initials: JR
authors.lastname: Mesquita
authors.forename: M São José
authors.initials: MS
authors.lastname: Nascimento
authors.forename: Lucília
authors.initials: L
authors.lastname: Saraiva
mesh_terms: D000818:Animals; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D036022:Capsid Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D004283:Dog Diseases / Q000175:diagnosis / Q000821:virology; D004285:Dogs; D015870:Gene Expression; D029322:Norovirus / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
chemical_list: D036022:Capsid Proteins