title: Sperm cryopreservation methods.
abstract: There are multiple clinical situations where cryopreservation of sperm is required including sperm banking prior to chemotherapy or radiation treatment for cancer, donor sperm for couples without a male partner, and various etiologies of male factor infertility. Bunge and Sherman presented the first pregnancy resulting from the thawing of cryopreserved sperm in 1953 (Bunge RJ, Nature 172:767-768, 1953). Since that time, cryopreservation techniques have continued to improve. Here, we describe protocols for the cryopreservation and thawing of semen and testicular tissues using a simple liquid nitrogen vapor technique.
authors.affiliation: IVF Laboratory, University Women's Healthcare, Louisville, KY, USA.
authors.forename: Tiffany
authors.initials: T
authors.lastname: Justice
authors.forename: Greg
authors.initials: G
authors.lastname: Christensen
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D008297:Male; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa; D013737:Testis