title: Morphological assessment of the luminal surface of olfactory epithelium in mice deficient in tissue plasminogen activator following bulbectomy.
abstract: OBJECTIVE
  This study aimed to investigate the function of tissue plasminogen activator in the olfactory epithelium of mice following neural injury.
  
  
  METHODS
  Transmission electron microscopy was used to study the changes in the morphology of the olfactory epithelium 1-7 days after surgical ablation of the olfactory bulb (bulbectomy).
  
  
  RESULTS
  Prior to bulbectomy, a uniformly fine material was observed within some regions of the olfactory epithelium of mice deficient in tissue plasminogen activator. At 2-3 days after bulbectomy, there were degenerative changes in the olfactory epithelium. At 5-7 days after bulbectomy, we noted drastic differences in olfactory epithelium morphology between mice deficient in tissue plasminogen activator and wild-type mice (comparisons were made using findings from a previous study). The microvilli seemed to be normal and olfactory vesicles and receptor neuron dendrites were largely intact in the olfactory epithelium of mice deficient in tissue plasminogen activator.
  
  
  CONCLUSIONS
  The tissue plasminogen activator plasmin system may inhibit the regeneration of the olfactory epithelium in the early stages following neural injury.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Makino
authors.forename: S
authors.initials: S
authors.lastname: Ookawara
authors.forename: S
authors.initials: S
authors.lastname: Madoiwa
authors.forename: Y
authors.initials: Y
authors.lastname: Ohta
authors.forename: T
authors.initials: T
authors.lastname: Ishikawa
authors.forename: K
authors.initials: K
authors.lastname: Katoh
authors.forename: S
authors.initials: S
authors.lastname: Takigami
authors.forename: T
authors.initials: T
authors.lastname: Kanazawa
authors.forename: O
authors.initials: O
authors.lastname: Matsuo
authors.forename: M
authors.initials: M
authors.lastname: Ichikawa
authors.forename: J
authors.initials: J
authors.lastname: Mimuro
authors.forename: Y
authors.initials: Y
authors.lastname: Sakata
authors.forename: K
authors.initials: K
authors.lastname: Ichimura
mesh_terms: D000818:Animals; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D009830:Olfactory Bulb / Q000502:physiology* / Q000601:surgery*; D009831:Olfactory Mucosa / Q000166:cytology / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D010959:Tissue Plasminogen Activator / Q000172:deficiency* / Q000502:physiology
chemical_list: D010959:Tissue Plasminogen Activator