title: A selective luminescent probe for the direct time-gated detection of adenosine triphosphate.
abstract: A molecular probe for the luminescent detection of adenosine nucleotides is presented. The probe, Tb-DOTAm-Phen, readily distinguishes among the three adenosine nucleotides in buffered aqueous conditions at neutral pH, a requirement for the direct monitoring of enzymatic reactions converting adenosine triphosphate (ATP) to adenosine diphosphate or adenosine monophosphate. The probe is most efficient under millimolar concentrations of ATP which are relevant to intracellular conditions. Moreover, the long luminescence lifetime of the probe readily enables time-gating experiments.
authors.affiliation: Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, USA.
authors.forename: Evan A
authors.initials: EA
authors.lastname: Weitz
authors.forename: Jennifer Y
authors.initials: JY
authors.lastname: Chang
authors.forename: Adam H
authors.initials: AH
authors.lastname: Rosenfield
authors.forename: Valerie C
authors.initials: VC
authors.lastname: Pierre
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000737:chemistry*; D015195:Drug Design; D049408:Luminescent Agents / Q000737:chemistry*; D008665:Metalloporphyrins / Q000737:chemistry*; D015336:Molecular Probe Techniques; D013379:Substrate Specificity; D013997:Time Factors
chemical_list: D049408:Luminescent Agents; D008665:Metalloporphyrins; C578396:Tb-DOTAm-Phen; D000255:Adenosine Triphosphate