title: Construction of GPx active centers on natural protein nanodisk/nanotube: a new way to develop artificial nanoenzyme.
abstract: Construction of catalytic centers on natural protein aggregates is a challenging topic in biomaterial and biomedicine research. Here we report a novel construction of artificial nanoenzyme with glutathione peroxidase (GPx)-like function. By engineering the surface of tobacco mosaic virus (TMV) coat protein, the main catalytic components of GPx were fabricated on TMV protein monomers. Through direct self-assembly of the functionalized viral coat proteins, the multi-GPx centers were installed on these well-defined nanodisks or nanotubes. With the help of muti-selenoenzyme centers, the resulting organized nanoenzyme exhibited remarkable GPx activity, even approaching the level of natural GPx. The antioxidation study on subcell mitochondrial level demonstrated that virus-based nanoenzyme exerted excellent capacity for protecting cell from oxidative damage. This strategy represents a new way to develop artificial nanoenzymes.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, China.
authors.forename: Chunxi
authors.initials: C
authors.lastname: Hou
authors.forename: Quan
authors.initials: Q
authors.lastname: Luo
authors.forename: Jinliang
authors.initials: J
authors.lastname: Liu
authors.forename: Lu
authors.initials: L
authors.lastname: Miao
authors.forename: Chunqiu
authors.initials: C
authors.lastname: Zhang
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Gao
authors.forename: Xiyu
authors.initials: X
authors.lastname: Zhang
authors.forename: Jiayun
authors.initials: J
authors.lastname: Xu
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Dong
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry* / Q000648:ultrastructure; D005979:Glutathione Peroxidase / Q000737:chemistry* / Q000648:ultrastructure; D008422:Materials Testing; D018716:Molecular Mimicry; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D014027:Tobacco Mosaic Virus / Q000737:chemistry*
chemical_list: D036022:Capsid Proteins; D005979:Glutathione Peroxidase