title: In vitro activity of aminosterols against dermatophytes.
abstract: We recently reported that aminosterols are fungicidal due to their disrupting the outer membranes of yeasts and that they have a significant in vitro activity against various mould species. Yet, their activity against dermatophytes had never been tested. This study's objective was to evaluate the in vitro activity of squalamine and a synthetic aminosterol derivative (ASD) against various dermatophytes. Susceptibility testing of squalamine, ASD, terbinafine, and griseofulvin was performed, in triplicate, in accord with the Clinical Laboratory and Standards Institute's M38-A2 procedure, using an 80% growth inhibition endpoint. The studies included the following dermatophytes: Trichophyton rubrum, T. mentagrophytes, T. soudanense, Microsporum canis, M. audouinii, M. persicolor; M. cookie and M. gypseum. Squalamine and ASD showed significant in vitro activity against these dermatophytes. The minimum inhibitory concentrations (MICs) ranged from 4-16 mg/l and from 2-8 mg/l for squalamine and ASD, respectively. These findings support further clinical studies of aminosterols activity against superficial dermatophyte infections.
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Aix-Marseille Université, AP-HM, CHU Timone, Marseille CEDEX 05, France.
authors.forename: Oumar
authors.initials: O
authors.lastname: Coulibaly
authors.forename: Kamel
authors.initials: K
authors.lastname: Alhanout
authors.forename: Coralie
authors.initials: C
authors.lastname: L'Ollivier
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Brunel
authors.forename: Mahamadou A
authors.initials: MA
authors.lastname: Thera
authors.forename: Abdoulaye A
authors.initials: AA
authors.lastname: Djimdé
authors.forename: Ogobara K
authors.initials: OK
authors.lastname: Doumbo
authors.forename: Renaud
authors.initials: R
authors.lastname: Piarroux
authors.forename: Stéphane
authors.initials: S
authors.lastname: Ranque
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects* / Q000302:isolation & purification; D002648:Child; D002777:Cholestanols / Q000494:pharmacology*; D006118:Griseofulvin / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D009281:Naphthalenes / Q000494:pharmacology; D000077291:Terbinafine; D014006:Tinea Capitis / Q000382:microbiology
chemical_list: D000935:Antifungal Agents; D002777:Cholestanols; D009281:Naphthalenes; D006118:Griseofulvin; D000077291:Terbinafine