title: Synthesis and evaluation of naphthoic acid derivatives as fluorescent probes to screen advanced glycation end-products breakers.
abstract: Advanced glycation end-products, namely AGEs, are involved in the pathogenesis of numerous diseases. If AGEs inhibitors are well-known, only few products are described as compounds able to destroy those deleterious products. In this work, we describe naphthoic acid derivatives, particularly 1-(naphthalen-1-yl)propane-1,2-dione 9, allowing the simple and rapid detection of AGEs breakers using a 96-well microplate fluorescence assay. Since the inaugurate publication about AGEs breakers whose activity was demonstrated using HPLC analysis, this work proposes the first assay suitable for automated and high throughput screening of AGEs breakers.
authors.affiliation: PRES LUNAM, Université d'Angers, EA 921 SONAS, SFR 4207 QUASAV, 16 bd Daviers, 49045 Angers, Cedex 01, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Séro
authors.forename: François
authors.initials: F
authors.lastname: Calard
authors.forename: Lionel
authors.initials: L
authors.lastname: Sanguinet
authors.forename: Eric
authors.initials: E
authors.lastname: Levillain
authors.forename: Pascal
authors.initials: P
authors.lastname: Richomme
authors.forename: Denis
authors.initials: D
authors.lastname: Séraphin
authors.forename: Séverine
authors.initials: S
authors.lastname: Derbré
mesh_terms: D001681:Biological Assay; D002264:Carboxylic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D009281:Naphthalenes / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: C579013:1-(naphthalen-1-yl)propane-1,2-dione; D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D017127:Glycation End Products, Advanced; D009281:Naphthalenes; C085312:1-naphthoic acid