title: Transmitted HIV type 1 drug resistance in newly diagnosed Cuban patients.
abstract: Knowledge of the associated mutations to transmitted drug resistance (TDR) in strains of human immunodeficiency virus type 1 (HIV-1) constitutes a fundamental premise in epidemiological surveillance. In this present study, TDR from 200 Cuban patients who were diagnosed with HIV-1 between 2009 and 2011 was analyzed. By partial reverse transcriptase polymerase chain reaction (RT-PCR) and sequencing of the HIV pol gene, an HIV subtype and transmitted resistance profile were determined. The prevalence of associated mutations to the TDR in the individuals studied was 21.5%. In the region of the reverse transcriptase, the most common mutations were K103N and M184V, while in the region of the protease they were L33F and M46L. The results of this study provide evidence of TDR in the Cuban seropositive population and suggest the necessity of making resistance assays before beginning antiretroviral therapy in HIV-1-infected patients in Cuba.
authors.affiliation: AIDS Research Laboratory, Carretera Tapaste y Autopista Nacional , San José de las Lajas, Mayabeque 32700, Cuba. liuberyans@infomed.sld.cu
authors.forename: Liuber Y
authors.initials: LY
authors.lastname: Machado
authors.forename: Marta
authors.initials: M
authors.lastname: Dubed
authors.forename: Héctor
authors.initials: H
authors.lastname: Díaz
authors.forename: Nancy
authors.initials: N
authors.lastname: Ruiz
authors.forename: Dania
authors.initials: D
authors.lastname: Romay
authors.forename: Neysi
authors.initials: N
authors.lastname: Váldes
authors.forename: Madeline
authors.initials: M
authors.lastname: Blanco
authors.forename: Eladio
authors.initials: E
authors.lastname: Silva
mesh_terms: D000328:Adult; D003462:Cuba; D024882:Drug Resistance, Viral; D005260:Female; D015658:HIV Infections / Q000635:transmission* / Q000821:virology*; D016333:HIV Protease / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000235:genetics; D015497:HIV-1 / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D017422:Sequence Analysis, DNA; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D054302:pol Gene Products, Human Immunodeficiency Virus; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase; D016333:HIV Protease