title: Elucidation of a novel pathway through which HDAC1 controls cardiomyocyte differentiation through expression of SOX-17 and BMP2.
abstract: Embryonic Stem Cells not only hold a lot of potential for use in regenerative medicine, but also provide an elegant and efficient way to study specific developmental processes and pathways in mammals when whole animal gene knock out experiments fail. We have investigated a pathway through which HDAC1 affects cardiovascular and more specifically cardiomyocyte differentiation in ES cells by controlling expression of SOX17 and BMP2 during early differentiation. This data explains current discrepancies in the role of HDAC1 in cardiovascular differentiation and sheds light into a new pathway through which ES cells determine cardiovascular cell fate.
authors.affiliation: Feinberg Cardiovascular Research Institute, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America.
authors.forename: Eneda
authors.initials: E
authors.lastname: Hoxha
authors.forename: Erin
authors.initials: E
authors.lastname: Lambers
authors.forename: John A
authors.initials: JA
authors.lastname: Wasserstrom
authors.forename: Alexander
authors.initials: A
authors.lastname: Mackie
authors.forename: Veronica
authors.initials: V
authors.lastname: Ramirez
authors.forename: Tatiana
authors.initials: T
authors.lastname: Abramova
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Verma
authors.forename: Prasanna
authors.initials: P
authors.lastname: Krishnamurthy
authors.forename: Raj
authors.initials: R
authors.lastname: Kishore
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D024002:HMGB Proteins / Q000235:genetics* / Q000378:metabolism; D056284:Histone Deacetylase 1 / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008957:Models, Genetic; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055760:SOXF Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D013997:Time Factors
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D024002:HMGB Proteins; D055760:SOXF Transcription Factors; C511606:Sox17 protein, mouse; C484389:Hdac1 protein, mouse; D056284:Histone Deacetylase 1