title: [Comparison of effects of kaempferide and anhydroicaritin on biomineralization of cultured osteoblasts].
abstract: This study is to compare the effects of kaempferide and anhydroicaritin on biomineralization of rat osteoblasts (ROB) in vitro. Calvarias were dissected aseptically from newborn SD rats, the osteoblasts were obtained by enzyme digestion and were cultured in MEM containing 10% FBS. The medium was changed every three days, and serial subculture was performed when cells covered with 90% of the dish. Kaempferide and anhydroicaritin were separately added with final concentrations of 1 x 10(-4), 1 x 10(-5), 1 x 10(-6) and 1 x 10(-7) mol x L(-1) under the conditions of osteogenic differentiation. The proliferation was measured by MTT, and the optimal concentration was detected by the ALP activity at the 9th day after osteogenic induction culture. The osteogenic indexes of kaempferide, anhydroicaritin and control group with the optimal concentration were compared. The result showed that the anhydroicaritin at concentration of 1 x 10(-5) mol x L(-1) had significantly promoted the activity of ALP, calcium content and osteocalcin content, increased the number of CFU-F(ALP) and mineralized nodules, enhanced the mRNA level of BMP-2, OSX and Runx-2, which are key genes of osteogenic differentiation, and raised the protein content of collagen-I. However, the kaempferide group had not significantly represented the ability that promoted osteogenic differentiation of ROB. The difference of osteogenic differentiation on ROB between kaempferide and anhydroicaritin was caused by the prenyl group on C-8 of icariin.
authors.affiliation: Institute of Orthopaedics, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Song
authors.forename: Juan
authors.initials: J
authors.lastname: Yao
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Ma
authors.forename: Bao-Feng
authors.initials: BF
authors.lastname: Ge
authors.forename: Ke-Ming
authors.initials: KM
authors.lastname: Chen
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Guo
authors.forename: Xiang
authors.initials: X
authors.lastname: Lü
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D044949:Kaempferols / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001578:Benzopyrans; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D044949:Kaempferols; D012333:RNA, Messenger; C493717:Runx2 protein, rat; C495181:Sp7 protein, rat; D014157:Transcription Factors; C578157:anhydroicaritin; D015675:Osteocalcin; C449720:kaempferide; D000469:Alkaline Phosphatase; D002118:Calcium