title: Low-level visible light (LLVL) irradiation promotes proliferation of mesenchymal stem cells.
abstract: Low-level visible light irradiation was found to stimulate proliferation potential of various types of cells in vitro. Stem cells in general are of significance for implantation in regenerative medicine. The aim of the present study was to investigate the effect of low-level light irradiation on the proliferation of mesenchymal stem cells (MSCs). MSCs were isolated from the bone marrow, and light irradiation was applied at energy densities of 2.4, 4.8, and 7.2 J/cm(2). Illumination of the MSCs resulted in almost twofold increase in cell number as compared to controls. Elevated reactive oxygen species and nitric oxide production was also observed in MSCs cultures following illumination with broadband visible light. The present study clearly demonstrates the ability of broadband visible light illumination to promote proliferation of MSCs in vitro. These results may have an important impact on wound healing.
authors.affiliation: Department of Chemistry, Kanbar Laboratory for Nanomaterials, Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan 52900, Israel.
authors.forename: Anat
authors.initials: A
authors.lastname: Lipovsky
authors.forename: Uri
authors.initials: U
authors.lastname: Oron
authors.forename: Aharon
authors.initials: A
authors.lastname: Gedanken
authors.forename: Rachel
authors.initials: R
authors.lastname: Lubart
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000528:radiation effects; D002478:Cells, Cultured; D008027:Light; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000528:radiation effects*; D009569:Nitric Oxide / Q000096:biosynthesis; D010789:Phototherapy / Q000379:methods; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D017382:Reactive Oxygen Species; D009569:Nitric Oxide