title: Antitrypanosomal alkaloids from the marine bacterium Bacillus pumilus.
abstract: Fractionation of the ethyl acetate extract of the marine bacterium Bacillus pumilus isolated from the black coral Antipathes sp. led to the isolation of five compounds: cyclo-(L-Leu-L-Pro) (1), 3-hydroxyacetylindole (2), N-acetyl-β-oxotryptamine (3), cyclo-(L-Phe-L-Pro) (4), and 3-formylindole (5). The structures of compounds 1-5 were established by spectroscopic analyses, including HRESITOF-MS and NMR (1H, 13C, HSQC, HMBC and COSY). Compounds 2, 3 and 5 caused the inhibition on the growth of Trypanosoma cruzi (T. cruzi), with IC50 values of 20.6, 19.4 and 26.9 μM, respectively, with moderate cytotoxicity against Vero cells. Compounds 1-5 were found to be inactive when tested against Plasmodium falciparum and Leishmania donovani, therefore showing selectivity against T. cruzi parasites.
authors.affiliation: Center for Biodiversity and Drug Discovery, Institute for Scientific Research and High Technology Services, City of Knowledge, P.O. Box 0843-01103, Panama.
authors.forename: Sergio
authors.initials: S
authors.lastname: Martínez-Luis
authors.forename: José Félix
authors.initials: JF
authors.lastname: Gómez
authors.forename: Carmenza
authors.initials: C
authors.lastname: Spadafora
authors.forename: Héctor M
authors.initials: HM
authors.lastname: Guzmán
authors.forename: Marcelino
authors.initials: M
authors.lastname: Gutiérrez
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D037421:Anthozoa / Q000382:microbiology; D001407:Bacillus / Q000302:isolation & purification / Q000378:metabolism*; D002460:Cell Line; D002522:Chlorocebus aethiops; D006801:Humans; D007893:Leishmania donovani / Q000187:drug effects; D021261:Parasitic Sensitivity Tests; D010963:Plasmodium falciparum / Q000187:drug effects; D014344:Trypanocidal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects*; D014709:Vero Cells
chemical_list: D000470:Alkaloids; D014344:Trypanocidal Agents