title: New microsatellite markers for the abalone Haliotis  midae developed by 454 pyrosequencing and in silico analyses.
abstract: Farming of Haliotis midae is the most lucrative aquaculture venture in South Africa. The genome of this species needs to be studied to assist in selective breeding programs aimed at increasing overall yield, and molecular markers will be required to attain this goal. We identified and characterized 82 polymorphic microsatellite loci by using repeat-enriched genomic libraries and high-throughput pyrosequencing technology. The observed number of alleles ranged from 2 to 21, expected heterozygosity from 0.063 to 0.968, observed heterozygosity from 0.000 to 1.000, and polymorphic information content from 0.059 to 0.934. Three loci gave significant hits to other haliotid genes and/or microsatellite loci; hits to genes were always located in the 5'/3'-UTR or intron region. Many of these newly designed markers would be useful for parentage, population and linkage studies.
authors.affiliation: Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Matieland, South Africa. rslabbrt@sun.ac.za
authors.forename: R
authors.initials: R
authors.lastname: Slabbert
authors.forename: J-A
authors.initials: JA
authors.lastname: Hepple
authors.forename: C
authors.initials: C
authors.lastname: Rhode
authors.forename: A E
authors.initials: AE
authors.lastname: Bester-Van der Merwe
authors.forename: R
authors.initials: R
authors.lastname: Roodt-Wilding
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods*; D017931:DNA Primers / Q000378:metabolism; D049851:Gastropoda / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*; D013223:Statistics as Topic; D013696:Temperature
chemical_list: D017931:DNA Primers