title: Identification of irradiated meats by determining o- and m-tyrosine as markers.
abstract: To identify the irradiated meats, various parameters that affect extraction efficiency of tyrosine positional isomers were evaluated. The optimum procedure employed simple extraction by 0.1% formic acid and protein precipitation by acetone. Baseline separation for the extract was carried out on LC-fluorescence detection (FLD) and LC-tandem mass spectrometry (MS/MS). The LC-FLD and LC-MS/MS method had LOD of 1.7-2.1 and 0.3-0.5 ng/mL, respectively, and showed excellent linear correlation over three orders of magnitude, obtained ideal recoveries (78.68-88.90%) and RSD (≤ 8%). The methods were successfully applied in multiple samples. For o- and m-tyrosine, the order of descending trend was: chicken>beef > hairtail > pork and chicken > hairtail > beef > pork, respectively. The radiation dose could be quantitatively evaluated by the nonlinear correlation (y = A(0) x (2) + A(1)x + A(2)) with coefficients of determination r(2) > 0.998 in individual meat samples.
authors.affiliation: College of Food Science and Biological Engineering, Zhejiang Gongshang University, Hangzhou, PR China.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.forename: Lihua
authors.initials: L
authors.lastname: Fan
authors.forename: Jun
authors.initials: J
authors.lastname: Song
authors.forename: Chenchen
authors.initials: C
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000096:Acetone / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods; D004307:Dose-Response Relationship, Radiation; D005453:Fluorescence; D005514:Food Irradiation / Q000379:methods*; D016014:Linear Models; D008460:Meat / Q000032:analysis*; D011506:Proteins / Q000378:metabolism; D015203:Reproducibility of Results; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods; D014443:Tyrosine / Q000032:analysis*
chemical_list: D011506:Proteins; D000096:Acetone; D014443:Tyrosine; C026741:2-tyrosine; C015566:3-tyrosine