title: Erythropoietin ameliorates oxidative stress and tissue injury following renal ischemia/reperfusion in rat kidney and lung.
abstract: OBJECTIVE
  To study the effect of erythropoietin (EPO) treatment on renal and lung injury following renal ischemia/reperfusion (I/R).
  
  
  METHODS
  Thirty male Wistar rats were assigned to three groups of 10 rats each. The first group was sham-operated, the second was subjected to renal I/R (30 min of ischemia followed by 24 h of reperfusion). The third group was subjected to renal I/R and treated with EPO in two doses: the first dose 1 h prior to ischemia (1,000 U/kg) and the second dose 6 h after ischemia (1,000 U/kg).
  
  
  RESULTS
  The renal and lung tissue injury index, tissue serum blood urea nitrogen and creatinine (Cr) were higher in the renal I/R group compared to the renal I/R + EPO group; the difference was statistically significant (p < 0.05). Kidney and lung tissue glutathione peroxidase and superoxide dismutase levels were higher in the renal I/R + EPO group than the renal I/R group; the difference was also statistically significant (p < 0.05).
  
  
  CONCLUSIONS
  The data showed that EPO pretreatment could be effective in reducing renal and lung injury following renal I/R and could improve the cellular antioxidant defense system. Hence EPO pretreatment may be effective for attenuating renal and lung injury after renal I/R-induced injury during surgical procedures, hypotension, renal transplantation and other conditions inducing renal I/R.
authors.affiliation: Department of Nephrology, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ardalan
authors.forename: Rasoul
authors.initials: R
authors.lastname: Estakhri
authors.forename: Babak
authors.initials: B
authors.lastname: Hajipour
authors.forename: Khalil
authors.initials: K
authors.lastname: Ansarin
authors.forename: Naser Ahmadi
authors.initials: NA
authors.lastname: Asl
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Nasirizade
authors.forename: Alireza Nour
authors.initials: AN
authors.lastname: Azar
authors.forename: Amir
authors.initials: A
authors.lastname: Ghorbanihaghjou
authors.forename: Amir Mansour
authors.initials: AM
authors.lastname: Vatankhah
authors.forename: Heydar Ali
authors.initials: HA
authors.lastname: Esmaili
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D004921:Erythropoietin / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007674:Kidney Diseases / Q000517:prevention & control*; D008171:Lung Diseases / Q000517:prevention & control*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D004921:Erythropoietin; D003404:Creatinine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase