title: Tuf and secY PCR amplification and genotyping of phytoplasmas.
abstract: Tuf and secY genotyping techniques have been developed to distinguish phytoplasma strains. Tuf polymerase chain reaction sequence analyses are available for phytoplasma taxonomic groups 16SrI, 16SrV, 16SrXII-A, and XII-B. In addition to their use to confirm the taxonomic status of phytoplasma strains, they allow the spread of phytoplasma strains in host plants and insect vectors to be traced. SecY is more variable than tuf and is therefore more discriminatory than tuf, but secY and tuf phylogenies show congruence.
authors.affiliation: UMR1332 Fruit Biology and Pathology, INRA, University of Bordeaux, Villenave d'Ornon, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Foissac
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Danet
authors.forename: Sylvie
authors.initials: S
authors.lastname: Malembic-Maher
authors.forename: Pascal
authors.initials: P
authors.lastname: Salar
authors.forename: Dana
authors.initials: D
authors.lastname: Safářová
authors.forename: Pavla
authors.initials: P
authors.lastname: Válová
authors.forename: Milan
authors.initials: M
authors.lastname: Navrátil
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D005838:Genotype; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010802:Phylogeny; D045582:Phytoplasma / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D010444:Peptide Elongation Factor Tu