title: In situ SVVYGLR peptide conjugation into injectable gelatin-poly(ethylene glycol)-tyramine hydrogel via enzyme-mediated reaction for enhancement of endothelial cell activity and neo-vascularization.
abstract: Tissue engineering therapies require biocompatible and bioactive biomaterials that are capable of encouraging an angiogenic response for effective tissue regeneration. In this study, a SVVYGLR peptide, which functions as a potent angiogenic factor, was conjugated into injectable gelatin-poly(ethylene glycol)-tyramine (GPT) hydrogels in situ to enhance endothelial cell activities and neo-vascularization. SVVYGLRGGY (SV-Y) conjugated GPT (SV-GPT) hydrogels were formed in situ via enzyme-mediated reaction using horseradish peroxidase (HRP) and hydrogen peroxide (H(2)O(2)). The physico-chemical properties were characterized and could be controlled depending on the feed peptide and H(2)O(2) concentration. The concentration of conjugated peptide ranged from 0.37 to 0.81 μmol/mL, and the elastic moduli (G') of the hydrogels were 600-4900 Pa. In vitro cell studies using human umbilical vein endothelial cells (HUVECs) and in vivo subcutaneous injection studies were performed to confirm the effect of the SVVYGLR peptide on HUVEC activity and neo-vascularization. Obtained results demonstrated that the in situ conjugation of SVVYGLR sequences into phenol residues of GPT hydrogels enhanced the activity of HUVECs in vitro and stimulated the formation of new blood vessels in the hydrogel matrices in vivo. From the results, we suggest that in situ conjugation of SV-Y to GPT hydrogels via the enzymatic reaction may be an efficient tool to prepare injectable bioactive hydrogels that can enhance endothelial cell activities and promoting angiogenesis for tissue regeneration.
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon, 443-749 Republic of Korea.
authors.forename: Kyung Min
authors.initials: KM
authors.lastname: Park
authors.forename: Yunki
authors.initials: Y
authors.lastname: Lee
authors.forename: Joo Young
authors.initials: JY
authors.lastname: Son
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Bae
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005780:Gelatin / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D007267:Injections; D055595:Mechanical Phenomena; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000737:chemistry*; D014439:Tyramine / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D009842:Oligopeptides; D011092:Polyethylene Glycols; D005780:Gelatin; D014439:Tyramine