title: Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344.
abstract: Pseudomonas pseudoalcaligenes CECT5344 is a Gram-negative bacterium able to tolerate cyanide and to use it as the sole nitrogen source. We report here the first draft of the whole genome sequence of a P. pseudoalcaligenes strain that assimilates cyanide. Three aspects are specially emphasized in this manuscript. First, some generalities of the genome are shown and discussed in the context of other Pseudomonadaceae genomes, including genome size, G + C content, core genome and singletons among other features. Second, the genome is analysed in the context of cyanide metabolism, describing genes probably involved in cyanide assimilation, like those encoding nitrilases, and genes related to cyanide resistance, like the cio genes encoding the cyanide insensitive oxidases. Finally, the presence of genes probably involved in other processes with a great biotechnological potential like production of bioplastics and biodegradation of pollutants also is discussed.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, 14071, Córdoba, Spain.
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Luque-Almagro
authors.forename: Felipe
authors.initials: F
authors.lastname: Acera
authors.forename: Ma Isabel
authors.initials: MI
authors.lastname: Igeño
authors.forename: Daniel
authors.initials: D
authors.lastname: Wibberg
authors.forename: Ma Dolores
authors.initials: MD
authors.lastname: Roldán
authors.forename: Lara P
authors.initials: LP
authors.lastname: Sáez
authors.forename: Magdalena
authors.initials: M
authors.lastname: Hennig
authors.forename: Alberto
authors.initials: A
authors.lastname: Quesada
authors.forename: Ma José
authors.initials: MJ
authors.lastname: Huertas
authors.forename: Jochen
authors.initials: J
authors.lastname: Blom
authors.forename: Faustino
authors.initials: F
authors.lastname: Merchán
authors.forename: Ma Paz
authors.initials: MP
authors.lastname: Escribano
authors.forename: Sebastian
authors.initials: S
authors.lastname: Jaenicke
authors.forename: Jessica
authors.initials: J
authors.lastname: Estepa
authors.forename: Ma Isabel
authors.initials: MI
authors.lastname: Guijo
authors.forename: Manuel
authors.initials: M
authors.lastname: Martínez-Luque
authors.forename: Daniel
authors.initials: D
authors.lastname: Macías
authors.forename: Rafael
authors.initials: R
authors.lastname: Szczepanowski
authors.forename: Gracia
authors.initials: G
authors.lastname: Becerra
authors.forename: Silvia
authors.initials: S
authors.lastname: Ramirez
authors.forename: Ma Isabel
authors.initials: MI
authors.lastname: Carmona
authors.forename: Oscar
authors.initials: O
authors.lastname: Gutiérrez
authors.forename: Isabel
authors.initials: I
authors.lastname: Manso
authors.forename: Alfred
authors.initials: A
authors.lastname: Pühler
authors.forename: Francisco
authors.initials: F
authors.lastname: Castillo
authors.forename: Conrado
authors.initials: C
authors.lastname: Moreno-Vivián
authors.forename: Andreas
authors.initials: A
authors.lastname: Schlüter
authors.forename: Rafael
authors.initials: R
authors.lastname: Blasco
mesh_terms: D000332:Aerobiosis / Q000235:genetics; D000595:Amino Acid Sequence; D000619:Aminohydrolases / Q000737:chemistry / Q000235:genetics; D001482:Base Composition / Q000235:genetics; D003486:Cyanides / Q000378:metabolism*; D023061:Gene Order; D059646:Genome Size / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D006899:Mixed Function Oxygenases / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D054813:Polyhydroxyalkanoates / Q000378:metabolism; D044205:Pseudomonas pseudoalcaligenes / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D026801:Synteny / Q000235:genetics
chemical_list: D003486:Cyanides; D054813:Polyhydroxyalkanoates; D006899:Mixed Function Oxygenases; C436954:cyanide oxidase; D000619:Aminohydrolases; C021867:nitrilase