title: [Distribution of puerarin contained in Zige freeze-dried powder injection in rat liver and kidney].
abstract: OBJECTIVE
  To investigate the distribution process of puerarin contained in Zige freeze-dried powder injection in rat liver and kidney and the safety of Zige freeze-dried powder injection.
  
  
  METHODS
  Rats were divided into the Zige freeze-dried powder injection group and the puerarin freeze-dried powder injection control group randomly. The liver and kidney samples were collected at 5, 10, 20, 30, 45, 60 and 120 min after intravenous administration of puerarin (26.7 microg x g(-1)) through caudal vein and detected by HPLC.
  
  
  RESULTS
  The concentration of puerarin in kidney reached the max value of 58.12 microg x g(-1) for the Zige freeze-dried powder injection group and 71.28 microg x g(-1) for the Puerarin freeze-dried powder injection group. The value of AUC(0-2h) was 26.24 microg x h x g(-1) for the Zige freeze-dried powder injection group and 35.24 microg x h x g(-1) for the puerarin freeze-dried powder injection group, MRT(0-2h) was 0. 39 h for the Zige freeze-dried powder injection group and 0. 42 h for the puerarin freeze-dried powder injection control group. Compared with the control group, the Zige freeze-dried powder injection group showed a significant decrease in Cmax and AUC(0-2h) (P < 0.05), with no notable difference in peak time tmax and MRT(0-2h). The two groups showed no obvious difference in tmax Cmax, AUC(0-2h) and MRT(0-2h) of puerarin in rat kidney.
  
  
  CONCLUSIONS
  Compared with Zige freeze-dried powder injection, Zige freeze-dried powder injection can reduce the distribution of puerarin in rat kidney, with no obvious change in the elimination of puerarin in rat kidney. It also showed no significant change in distribution and elimination in liver. This indicates that Zige freeze-dried powder injection is safer to kidney than puerarin freeze-dried powder injection.
authors.affiliation: College of Pharmacy, Southwest University Chongqing Pharmacodynamic Evaluation of Engineering Research Center, Chongqing 400715, China. wqin1127@tom.com
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.forename: Mao
authors.initials: M
authors.lastname: Xing
authors.forename: Lijuan
authors.initials: L
authors.lastname: Deng
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Kipaytul
authors.initials: K
authors.lastname: Mukaram
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D005612:Freeze Drying; D007267:Injections; D007529:Isoflavones / Q000008:administration & dosage* / Q000009:adverse effects / Q000493:pharmacokinetics*; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011208:Powders; D051381:Rats
chemical_list: D007529:Isoflavones; D011208:Powders; C033607:puerarin