title: Cochlin expression in vestibular endorgans obtained from patients with Meniere's disease.
abstract: The distribution of cochlin and its associated basement membrane proteins (collagen IV, collagen II, laminin-β2, and nidogen-1) were evaluated in the vestibular endorgans of subjects with Meniere's disease and compared with normal specimens. Cochlin mRNA expression in vestibular endorgans from Meniere's disease specimens was also investigated. Specimens were obtained from patients who had Meniere's disease and who were undergoing ablative labyrinthectomy. Control specimens were obtained both from autopsy specimens with documented normal audiovestibular function and from patients undergoing labyrinthectomy for acoustic neuroma excision. In the normal control specimens, cochlin immunoreactivity was found evenly distributed in the stroma of the cristae ampullaris and maculae of the utricle. In Meniere's specimens, cochlin immunoreactivity was markedly increased; this was associated with an increase in cochlin mRNA expression as shown by real-time reverse transcription with the polymerase chain reaction. Collagen IV and laminin-β2 immunoreactivity was significantly decreased in Meniere's specimens. Nidogen-1 and collagen II immunoreactivity was unchanged in Meniere's specimens when compared with normal samples. Cochlin upregulation has been implicated in the hereditary audiovestibulopathy, DFNA9. The increased expression of cochlin and decreased expression of collagen IV and laminin in Meniere's disease are suggestive that the overexpression of cochlin contributes to the dysfunctional inner ear homeostasis seen in this disease.
authors.affiliation: Department of Head and Neck Surgery, UCLA School of Medicine David Geffen, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA.
authors.forename: Audrey P
authors.initials: AP
authors.lastname: Calzada
authors.forename: Ivan A
authors.initials: IA
authors.lastname: Lopez
authors.forename: Luis
authors.initials: L
authors.lastname: Beltran Parrazal
authors.forename: Akira
authors.initials: A
authors.lastname: Ishiyama
authors.forename: Gail
authors.initials: G
authors.lastname: Ishiyama
mesh_terms: D000369:Aged, 80 and over; D000818:Animals; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D008297:Male; D008575:Meniere Disease / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D051379:Mice; D051381:Rats; D014722:Vestibule, Labyrinth / Q000378:metabolism* / Q000473:pathology
chemical_list: C110668:COCH protein, human; D016326:Extracellular Matrix Proteins