title: [Saline solution as culture media from a viewpoint of nosocomial bacteremia].
abstract: BACKGROUND
  Nutrient-containing aqueous solutions for parenteral use are susceptible of microbial contamination, beeing an important cause of serious infectious complications. Objective. To determine the capacity of organisms for growing in saline as compared with dextrose solution and tri-destilled sterile water.
  
  
  METHODS
  Experimental controlled study. Different microbial strains were innoculated in aqueous solutions (5% dextrose and 0.9% saline) as well as tri-destilled sterile water. Results were analized using the ANOVA test for repeated measurements.
  
  
  RESULTS
  In 0.9% saline solution, all the Enterobacteriaceae strains tested had a significant increase in their bacterial quantification in the analized time period (F = 8.35, p = 0.0145). For most organisms, the growth was even better than the one observed in nutrient-containing solution.
  
  
  CONCLUSIONS
  The 0.9% saline solution can support significative growing of potentially pathogenic bacteria. We recommend a strict compliance to the good nursing standards when handling this kind of solutions.
authors.affiliation: Universidad de Guanajuato y Hospital Regional de Alta Especialidad del Bajío.
authors.forename: Verónica Vanesa
authors.initials: VV
authors.lastname: Infante
authors.forename: Ana María
authors.initials: AM
authors.lastname: Cano
authors.forename: Humberto
authors.initials: H
authors.lastname: Medina Valdovinos
authors.forename: Alejandro E
authors.initials: AE
authors.lastname: Macías
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Alvarez
mesh_terms: D016470:Bacteremia / Q000382:microbiology*; D001419:Bacteria / Q000254:growth & development*; D003428:Cross Infection / Q000382:microbiology*; D004340:Drug Contamination; D012965:Sodium Chloride
chemical_list: D012965:Sodium Chloride