title: Trichosporon vanderwaltii sp. nov., an asexual basidiomycetous yeast isolated from soil and beetles.
abstract: During a survey of unidentified yeast isolates deposited in the UNESCO-MIRCEN Biotechnological Yeast Culture Collection housed at the Department of Microbial, Biochemical and Food Biotechnology of the University of the Free State, one isolate obtained from soil in South Africa showed 100 % identity in D1/D2 rDNA sequence with undescribed basidiomycetous yeasts isolated from the gut of beetles from the United States of America and forest soil from Taiwan in the NCBI sequence database. Phylogenetic analyses using sequences of the D1/D2 rDNA and ITS regions indicated that all these isolates form a well-supported sub-clade that is the sister clade to the Brassicae plus Porosum clades of Trichosporon in the order Trichosporonales. Subsequent phenotypic tests revealed that asexual reproduction by budding is rare but dominated by arthroconidia resulting from segmentation of hyphae and that fusiform giant cells are characterized by budding from a broad base. These findings further suggest that these isolates belong to a single tremellomycetous yeast species for which the name Trichosporon vanderwaltii CBS 12124(T) (=NRRL Y-48732(T), =UOFS Y-1920(T)) is proposed.
authors.affiliation: Department of Microbial, Biochemical, and Food Biotechnology, University of the Free State, PO Box 339, Bloemfontein, 9300, South Africa.
authors.forename: Thabiso E
authors.initials: TE
authors.lastname: Motaung
authors.forename: Jacobus
authors.initials: J
authors.lastname: Albertyn
authors.forename: Johan L F
authors.initials: JL
authors.lastname: Kock
authors.forename: Ching-Fu
authors.initials: CF
authors.lastname: Lee
authors.forename: Sung-Oui
authors.initials: SO
authors.lastname: Suh
authors.forename: Meredith
authors.initials: M
authors.lastname: Blackwell
authors.forename: Carolina H
authors.initials: CH
authors.lastname: Pohl
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D001517:Coleoptera / Q000382:microbiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013019:South Africa; D013624:Taiwan; D014250:Trichosporon / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D014481:United States
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer