title: Detection of metal residues on bone using SEM-EDS--part II: Sharp force injury.
abstract: Scanning electron microscopy coupled with X-ray energy dispersive spectrometry (SEM-EDS) is extensively seen in the literature for the improvement of the macroscopic analysis of sharp lesions, but very few indications concerning its real potential in the forensic context, particularly in forensic anthropology, are at the moment available. This paper represents a pilot study for the analysis of the behaviour of metallic residues found on bone after sharp force injury. Fourteen lesions were made on human bone (radius), cleaned of all soft tissues, with seven different sharp tools (three metal instruments, three metal saws and a baked-clay knife). Tools and lesions underwent SEM-EDS. From 3 to 18 particles were detected on each lesion, whose diameter was included between 0.5 and 150 μm. In 58% of cases, particle composition was concordant with the instrument used. The results seem to suggest that sharp force injury frequently leaves relatively few residues on bone, particularly in the case of common types of metal. Saws showed slightly more contamination with other residues than knives, which may be explained by the capability of the saw's teeth to retain the residues of previously encountered material. In addition, metal residues related to the tool used to cut the bone were located on the edges/walls of lesions in the case of saw marks, whereas they were more frequently found on the kerf floor in the case of knives/scissors, with the exception of the baked clay knife which when it leaves residues at all, seemed to leave them equally divided between the floor, the edges and the surrounding bone.
authors.affiliation: LABANOF, Laboratorio di Antropologia ed Odontologia Forense Sezione di Medicina Legale, Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Gibelli
authors.forename: D
authors.initials: D
authors.lastname: Mazzarelli
authors.forename: D
authors.initials: D
authors.lastname: Porta
authors.forename: A
authors.initials: A
authors.lastname: Rizzi
authors.forename: C
authors.initials: C
authors.lastname: Cattaneo
mesh_terms: D049429:Forensic Pathology; D006801:Humans; D019216:Metals, Heavy / Q000032:analysis*; D019565:Metals, Light / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D011884:Radius / Q000737:chemistry* / Q000648:ultrastructure; D012825:Silicon / Q000032:analysis*; D013052:Spectrometry, X-Ray Emission; D014950:Wounds, Penetrating / Q000473:pathology*
chemical_list: D019216:Metals, Heavy; D019565:Metals, Light; D012825:Silicon