title: Characterization of sugar beet pectic-derived oligosaccharides obtained by enzymatic hydrolysis.
abstract: Three pectic oligosaccharides (POS) obtained by enzymatic hydrolysis of sugar beet pectin by combining endopolygalacturonase and pectinmethylesterase, were characterized using high performance liquid chromatography, thermogravimetric analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry and X-ray diffraction. According to chromatographic analyses, POS are composed of mixture of polymers with different molecular weights and different galacturonic acid contents. The thermal analysis showed no major variation in thermal behavior regarding POS composition but showed that POS were more sensitive to thermal degradation than the parent pectin as well as the deesterified pectin. No change in composition of the gaseous products was obtained through TGA-FTIR analysis. The X-ray pattern of POS clearly indicated a considerable decrease in crystallinity when compared to the native pectin. Thus, thermal characterization of POS may have practical repercussions if the formulation in which POS is incorporated is submitted to a high temperature treatment.
authors.affiliation: University of Liège, Gembloux Agro-Bio Tech, Department of Industrial Biological Chemistry, Passage des déportés 2, B-5030 Gembloux, Belgium. ammcombo@student.ulg.ac.be
authors.forename: Agnan Marie Michel
authors.initials: AM
authors.lastname: Combo
authors.forename: Mario
authors.initials: M
authors.lastname: Aguedo
authors.forename: Nicolas
authors.initials: N
authors.lastname: Quiévy
authors.forename: Sabine
authors.initials: S
authors.lastname: Danthine
authors.forename: Dorothée
authors.initials: D
authors.lastname: Goffin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Jacquet
authors.forename: Christophe
authors.initials: C
authors.lastname: Blecker
authors.forename: Jacques
authors.initials: J
authors.lastname: Devaux
authors.forename: Michel
authors.initials: M
authors.lastname: Paquot
mesh_terms: D027461:Beta vulgaris / Q000737:chemistry*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry; D006358:Hot Temperature; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D010368:Pectins / Q000032:analysis / Q000737:chemistry*; D011096:Polygalacturonase / Q000737:chemistry
chemical_list: D009844:Oligosaccharides; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase; D011096:Polygalacturonase