title: Identification of clinical isolates of α-hemolytic streptococci by 16S rRNA gene sequencing, matrix-assisted laser desorption ionization-time of flight mass spectrometry using MALDI Biotyper, and conventional phenotypic methods: a comparison.
abstract: Fifty-six α-hemolytic streptococcal isolates were identified using MALDI Biotyper MS (Bruker Daltonics), API 20 Strep (bioMérieux), and BD Phoenix (Becton, Dickinson). The gold standard for identification was 16S rRNA gene sequence analysis with 16S-23S rRNA intergenic spacer sequencing. The following percentages of isolates were correctly identified to the species level: MALDI Biotyper, 46%; BD Phoenix, 35%; and API 20 Strep, 26%.
authors.affiliation: Medical Microbiology and Infectious Diseases, Institute of Life Science, The College of Medicine, Swansea University, Swansea, United Kingdom. angharad.p.davies@swansea.ac.uk
authors.forename: Angharad Puw
authors.initials: AP
authors.lastname: Davies
authors.forename: Michelle
authors.initials: M
authors.lastname: Reid
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Hadfield
authors.forename: Stuart
authors.initials: S
authors.lastname: Johnston
authors.forename: Jane
authors.initials: J
authors.lastname: Mikhail
authors.forename: Llinos G
authors.initials: LG
authors.lastname: Harris
authors.forename: Howard F
authors.initials: HF
authors.lastname: Jenkinson
authors.forename: Nidhika
authors.initials: N
authors.lastname: Berry
authors.forename: Ann M
authors.initials: AM
authors.lastname: Lewis
authors.forename: Khalid
authors.initials: K
authors.lastname: El-Bouri
authors.forename: Dietrich
authors.initials: D
authors.lastname: Mack
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D021901:DNA, Intergenic / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology*; D013291:Streptococcus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S