title: Cathepsins in the kidney of olive flounder, Paralichthys olivaceus, and their responses to bacterial infection.
abstract: Cathepsin activities are responsible for mediating various pathways involved in immune response, including the apoptosis pathway, toll-like receptor (TLR) signaling, cytokine induction and activation of granule serine proteases. In the present study, we investigated cathepsin responses in the kidneys of olive flounder infected with Streptococcus parauberis, analyzing cathepsin expression using a label-free, quantitative proteomic approach in conjunction with quantitative real-time polymerase chain reaction (qRT-PCR). In proteomic analyses, we detected cathepsin B, D, L and S proteins, noting significant decreases and increases in cathepsins B and L, respectively, with infection. Taken together with an evaluation of cathepsin B, D, F, K, L, S and X gene expression in normal and infected kidneys by qRT-PCR, our results indicate that cathepsins B, D, L and S are the dominant lysosomal proteases in the immune system of the teleostei, olive flounder. Cathepsins F, K and X were regarded as minor cathepsins.
authors.affiliation: Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea.
authors.forename: In Seok
authors.initials: IS
authors.lastname: Cha
authors.forename: Joseph
authors.initials: J
authors.lastname: Kwon
authors.forename: Ji Yeong
authors.initials: JY
authors.lastname: Mun
authors.forename: Seong Bin
authors.initials: SB
authors.lastname: Park
authors.forename: Ho Bin
authors.initials: HB
authors.lastname: Jang
authors.forename: Seong Won
authors.initials: SW
authors.lastname: Nho
authors.forename: Carmelo S
authors.initials: CS
authors.lastname: del Castillo
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Hikima
authors.forename: Takashi
authors.initials: T
authors.lastname: Aoki
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Jung
mesh_terms: D000818:Animals; D002403:Cathepsins / Q000235:genetics* / Q000378:metabolism; D005393:Fish Diseases / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005432:Flounder / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D015870:Gene Expression / Q000276:immunology*; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate; D007668:Kidney / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards; D012015:Reference Standards; D013290:Streptococcal Infections / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D013291:Streptococcus / Q000502:physiology; D059467:Transcriptome
chemical_list: D029941:Fish Proteins; D012337:RNA, Ribosomal, 18S; D002403:Cathepsins