title: Expression of tissue levels of matrix metalloproteinases and their inhibitors in breast cancer.
abstract: BACKGROUND
  We examined mRNA expression for MMP-2, MMP-7, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 in human breast cancer tissues, and the association between their expression and clinicopathological variables.
  
  
  METHODS
  Breast tissue samples from 120 patients with breast cancer were available for this study. To determine mRNA expression for MMP-2, MMP-7, MMP-9, MT1-MMP, TIMP-1, and TIMP-2, semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was carried out on tumor and normal tissues, respectively.
  
  
  RESULTS
  Mean MMP-2, MMP-7, MMP-9, MT1-MMP, TIMP-1, and TIMP-2 mRNA expression in the breast cancer was significantly higher than in the normal tissue. In terms of tumor size and lymph node metastasis of breast cancer, the differences in MMP-2, MMP-7, MMP-9, and MT1-MMP mRNA expression levels were significant.
  
  
  CONCLUSIONS
  The association between the increased expression of MMP-2, MMP-7, MMP-9, and MTI-MMP and clinicopathological parameters reflects a role in predicting the aggressive behavior of breast cancer.
authors.affiliation: Department of Breast Surgery, Affiliated Third Hospital of Harbin Medical University, 6 Baojian Road, Harbin, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Teng
authors.forename: Xin-xin
authors.initials: XX
authors.lastname: Guo
authors.forename: Zhi-gao
authors.initials: ZG
authors.lastname: Li
authors.forename: Ji-guang
authors.initials: JG
authors.lastname: Han
authors.forename: Lei
authors.initials: L
authors.lastname: Yao
mesh_terms: D000328:Adult; D000368:Aged; D001940:Breast / Q000737:chemistry; D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology*; D002277:Carcinoma / Q000737:chemistry / Q000235:genetics* / Q000556:secondary*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008207:Lymphatic Metastasis; D053511:Matrix Metalloproteinase 14 / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D020783:Matrix Metalloproteinase 7 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D020782:Matrix Metalloproteinases / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000032:analysis; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics*; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D019716:Tissue Inhibitor of Metalloproteinase-2; D020782:Matrix Metalloproteinases; D020783:Matrix Metalloproteinase 7; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D053511:Matrix Metalloproteinase 14