title: A rapid genotyping assay for segregating human olfactory receptor pseudogenes.
abstract: Variation in odor perception between individuals is initiated by binding of "odorant" molecules to olfactory receptors (ORs) located in the nasal cavity. To determine the mechanism for variation in odor perception, identification of specific ligands for a large number of ORs is required. However, it has been difficult to identify specific ligands, and ligands have been identified for only 2-3% of the hundreds of mammalian ORs. One way to increase the number of identified ligands is to take advantage of >60 human OR genes that are segregating as a result of a single nucleotide polymorphism, between a functional intact allele and a nonfunctional pseudogene allele. Potential ligands for these ORs can be identified by correlating odor perception of an individual with their genotype [intact/intact (I/I) vs. pseudogene/pseudogene (P/P)] for an OR gene. For this type of study, genotypes must be determined for a large number of individuals. We have developed a PCR-based assay to distinguish between the intact and pseudogene alleles of 49 segregating human OR genes and to determine an individual's genotype for these genes. To facilitate rapid determination of genotypes for a large number of individuals, the assay uses a small number of simple steps and equipment commonly found in most molecular biology and biochemistry laboratories. Although this assay was developed to distinguish between polymorphisms in OR genes, it can easily be adapted for use in distinguishing single nucleotide polymorphisms in any gene or chromosomal locus.
authors.affiliation: Department of Biological Sciences, Kingsborough Community College, Brooklyn, New York 11235, USA. chinkley@kbcc.cuny.edu
authors.forename: Craig S
authors.initials: CS
authors.lastname: Hinkley
authors.forename: Lindita
authors.initials: L
authors.lastname: Ismaili
mesh_terms: D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D015894:Genome, Human; D060005:Genotyping Techniques; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011544:Pseudogenes; D018035:Receptors, Odorant / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; C099706:OR3A1 protein, human; D018035:Receptors, Odorant; D004247:DNA