title: Covariation of copy number located at 16q22.1: new evidence in mammary ductal carcinoma.
abstract: Copy number variation (CNV) is crucial for gene regulation in humans. A             number of studies have revealed that CNV contributes to the initiation and progression             of cancer. In this study, we analysed four breast cancer cell lines and six fresh             frozen tissues from patients to evaluate the CNV present in the genome using microarray-based             comparative genomic hybridization (aCGH). Six genes located at 16q22.1 were analysed             by real-time PCR. The real-time PCR analysis revealed that the loss of CDH1/E2F4             may be associated with worse clinical and pathological findings. Interestingly,             covariation of CDH1, CDH3, CTCF and E2F4 was found to be associated with triple             negative breast cancer and HER-2 receptor status. In conclusion, our study supports             the idea that CNV at 16q22.1 in breast cancer is a frequent event; furthermore,             it reveals the covariation of CDH1, CDH3, CTCF and E2F4. The role of the covariation             is more complex than a simple additive effect of these four separate genes, which             may provide a novel target for breast cancer.
authors.affiliation: Department of Breast Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin 150081, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Yan-Mei
authors.initials: YM
authors.lastname: Yang
authors.forename: Shi-Hui
authors.initials: SH
authors.lastname: Yu
authors.forename: You-Xue
authors.initials: YX
authors.lastname: Zhang
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: He
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Sun
authors.forename: Xiao-Shuan
authors.initials: XS
authors.lastname: Liang
authors.forename: Da
authors.initials: D
authors.lastname: Pang
mesh_terms: D015703:Antigens, CD; D001943:Breast Neoplasms / Q000235:genetics*; D000076246:CCCTC-Binding Factor; D015820:Cadherins / Q000172:deficiency / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000235:genetics*; D045744:Cell Line, Tumor; D002869:Chromosome Aberrations; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D056915:DNA Copy Number Variations; D050704:E2F4 Transcription Factor / Q000172:deficiency / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012097:Repressor Proteins / Q000235:genetics
chemical_list: D015703:Antigens, CD; D000076246:CCCTC-Binding Factor; C513384:CDH1 protein, human; C512197:CDH3 protein, human; C000619697:CTCF protein, human; D015820:Cadherins; D050704:E2F4 Transcription Factor; C493762:E2F4 protein, human; D012097:Repressor Proteins