title: [Genotype I Japanese encephalitis virus is the main genotype in mosquito in Fujian province].
abstract: OBJECTIVE
  To grasp the infection rate and genotypes of Japanese encephalitis virus (JEV) in mosquito in Fujian province.
  
  
  METHODS
  Mosquito specimens in Sanming city, Jianyang city and Fuzhou city in Fujian province were collected in 2010. RT-PCR was used to detect the JEV sequence from the mosquitoes by specific primers. The sequence splicing and the differentiation analysis for nucleotides, deduced amino acid sequence and phylogenetic tree were performed by the software of ATGC, Clustal X (1.83), MegAlign, GeneDoc 3.2 and Mega (4.0).
  
  
  RESULTS
  Totally 6987 mosquitoes were collected and main species was Culex tritaeniorhynchus and Anopheles sinensis. The infection rate of JEV in mosquitoes in Sanming, Jianyang and Fuzhou were 1.25%, 1.76% and 0.65%, respectively. One full genome in the positive specimens was sequenced. And further study showed that the positive JEV sequences belonged to genotype I.
  
  
  CONCLUSIONS
  Genotype I Japanese encephalitis virus is the main genotype in mosquitos in Fujian province.
authors.affiliation: Department of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agriculture University, Hohhot 010018, China.
authors.forename: Xiao-Xi
authors.initials: XX
authors.lastname: He
authors.forename: Huan-Yu
authors.initials: HY
authors.lastname: Wang
authors.forename: Shi-Hong
authors.initials: SH
authors.lastname: Fu
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.forename: Fa-Zhu
authors.initials: FZ
authors.lastname: Yang
authors.forename: Wei-Xin
authors.initials: WX
authors.lastname: Chen
authors.forename: Bao-Hai
authors.initials: BH
authors.lastname: Xu
authors.forename: Su-Na
authors.initials: SN
authors.lastname: Lu
authors.forename: Han-Guo
authors.initials: HG
authors.lastname: Xie
authors.forename: Si Su-Rong
authors.initials: SS
authors.lastname: Ha
authors.forename: Yan-Sheng
authors.initials: YS
authors.lastname: Yan
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Liang
mesh_terms: D000818:Animals; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics*; D005838:Genotype; D010802:Phylogeny; D013997:Time Factors