title: Evaluation of in vivo antioxidant activity of Hericium erinaceus polysaccharides.
abstract: Hericium erinaceus polysaccharide (HEP) is a traditional Chinese medicine. In the present study, chemical composition and antioxidant activity of HEP was investigated. HPLC analysis showed that the HEP was composed of xylose (7.8%), ribose (2.7%), glucose (68.4%), arabinose (11.3%), galactose (2.5%) and mannose (5.2%). HEP was pre-administered to mice by gavage at a dose of 300 mg/kg for 15 days. Results found that HEP preadministration resulted in a significant decline in blood urea nitrogen (BUN), serum creatinine (Scr) and increase in creatinine clearance (CrCI) levels in HEP-pretreated group compared to renal ischemia reperfusion (IR) group. Malondialdehyde (MDA) level significantly increased, whereas Level of reduced glutathione (GSH) markedly decreased in renal IR animals. These results indicate that IR induced renal oxidative injury damage, as indicated by a increase in MDA level, and decrease in GSH level as well as the antioxidant enzymes activity. Such effects reflect that HEP can significantly decrease lipid peroxidation level and increase antioxidant enzymes activities in experimental animals.
authors.affiliation: Department of Urology, Luqiao Hospital of Taizhou Enze Medical Center (Group), Taizhou, Zhejiang Province 318050, PR China.
authors.forename: Zi-Hua
authors.initials: ZH
authors.lastname: Han
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Ye
authors.forename: Guan-Fu
authors.initials: GF
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D003404:Creatinine / Q000097:blood; D004353:Drug Evaluation, Preclinical; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D014508:Urea / Q000097:blood
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides; D008315:Malondialdehyde; D014508:Urea; D003404:Creatinine