title: Efficient antifouling surface for quantitative surface plasmon resonance based biosensor analysis.
abstract: Non-specific binding to biosensor surfaces is a major obstacle to quantitative analysis of selective retention of analytes at immobilized target molecules. Although a range of chemical antifouling monolayers has been developed to address this problem, many macromolecular interactions still remain refractive to analysis due to the prevalent high degree of non-specific binding. In this manuscript we explore the dynamic process of the formation of self-assembled monolayers and optimize physical and chemical properties thus reducing considerably non-specific binding while maintaining the integrity of the immobilized biomolecules. As a result, analysis of specific binding of analytes to immobilized target molecules is significantly facilitated.
authors.affiliation: LBPA, ENS de Cachan, CNRS, Cachan, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Nogues
authors.forename: Hervé
authors.initials: H
authors.lastname: Leh
authors.forename: Joseph
authors.initials: J
authors.lastname: Lautru
authors.forename: Olivier
authors.initials: O
authors.lastname: Delelis
authors.forename: Malcolm
authors.initials: M
authors.lastname: Buckle
mesh_terms: D000327:Adsorption; D057219:Biofouling / Q000517:prevention & control*; D004247:DNA / Q000378:metabolism; D061925:Immobilized Nucleic Acids / Q000378:metabolism; D019426:Integrases / Q000378:metabolism; D007700:Kinetics; D008958:Models, Molecular; D011485:Protein Binding; D012190:Retroviridae / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D061925:Immobilized Nucleic Acids; D004247:DNA; D019426:Integrases