title: Laboratory maintenance of rodent malaria parasites.
abstract: We provide a series of protocols that have been used for the cyclic transmission of rodent malaria parasites in the laboratory. This is now possible both in vivo and in vitro. We focus on the least "resource intensive" and generic methods that we find applicable to any parasite-host combination. Nonetheless, we recognize that the ability to construct transgenic "reporter" parasites/hosts now permits the use of elegant analytical and imaging technologies both in vitro, ex vivo, and in vivo in specific instances. The descriptions given illustrate methods routinely used for the maintenance of P. berghei; where critical, we note important differences when transmitting other parasite species.
authors.affiliation: The Malaria Centre, Department of Life Sciences, Imperial College, London, UK.
authors.forename: Chandra
authors.initials: C
authors.lastname: Ramakrishnan
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Delves
authors.forename: Kalpana
authors.initials: K
authors.lastname: Lal
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Blagborough
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Butcher
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Baker
authors.forename: Robert E
authors.initials: RE
authors.lastname: Sinden
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D009033:Culicidae / Q000469:parasitology; D004330:Drosophila; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D008018:Life Cycle Stages; D008288:Malaria / Q000469:parasitology*; D051379:Mice; D033661:Oocysts / Q000254:growth & development; D010962:Plasmodium berghei / Q000254:growth & development* / Q000302:isolation & purification; D051381:Rats; D012377:Rodentia / Q000469:parasitology*; D034101:Sporozoites / Q000502:physiology; D013194:Staining and Labeling / Q000379:methods