title: Establishment of a bioluminescent imaging-based in vivo leukemia model             by intra-bone marrow injection.
abstract: In vivo leukemia mouse models are usually generated by intraperitoneal (IP)             or intravenous (IV) injection of leukemia cells. However, the pattern of leukemia             development observed can be inconsistent. This study investigated injection directly             into bone marrow [intra-bone marrow transplantation (IBMT)], the natural microenvironment             of leukemia. A bioluminescent imaging-based leukemia animal model has been established             by direct injection of a bioluminescent leukemia cells (CCRF-CEM/fLuc) into NOD/SCID             mouse tibia bone marrow and compared with models established by IP and IV routes.             The comparison revealed that a bioluminescent in vivo leukemia model established             via IBMT could recapitulate leukemia more faithfully and facilitate improved quantification             of leukemia engraftment kinetics with a wider range of bioluminescent intensity             than IP or IV. IBMT of bioluminescent leukemic cells allowed quantification of             dose-dependent responses to anti-leukemic drugs, thus validating this model as             a potential preclinical anti-leukemic drug screening system. IBMT-leukemia cells             isolated from peripheral blood of the model mice and then injected into new recipients             successfully established a second generation IBMT in vivo model and demonstrated             the reproducibility of the model. Bioluminescent imaging-based analysis of this             IBMT-leukemia model could provide a means for the comprehensive evaluation of             treatment responses with enhanced sensitivity in preclinical studies.
authors.affiliation: Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
authors.forename: Myoung Woo
authors.initials: MW
authors.lastname: Lee
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Keon Hee
authors.initials: KH
authors.lastname: Yoo
authors.forename: Dae Seong
authors.initials: DS
authors.lastname: Kim
authors.forename: Jin Mo
authors.initials: JM
authors.lastname: Yang
authors.forename: Hye Ryung
authors.initials: HR
authors.lastname: Kim
authors.forename: Yoo Hun
authors.initials: YH
authors.lastname: Noh
authors.forename: Hyunjung
authors.initials: H
authors.lastname: Baek
authors.forename: Heechung
authors.initials: H
authors.lastname: Kwon
authors.forename: Meong Hi
authors.initials: MH
authors.lastname: Son
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Hee Won
authors.initials: HW
authors.lastname: Cheuh
authors.forename: Hye Lim
authors.initials: HL
authors.lastname: Jung
authors.forename: Ki Woong
authors.initials: KW
authors.lastname: Sung
authors.forename: Hong Hoe
authors.initials: HH
authors.lastname: Koo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D016026:Bone Marrow Transplantation; D045744:Cell Line, Tumor; D018450:Disease Progression; D015870:Gene Expression; D007942:Leukemia, Experimental / Q000175:diagnosis / Q000188:drug therapy / Q000473:pathology; D049409:Luciferases, Firefly / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D057054:Molecular Imaging; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D049409:Luciferases, Firefly