title: A simple and label-free electrochemical biosensor for DNA detection based on the super-sandwich assay.
abstract: The focus of this work was on designing a label-free DNA biosensor based on a super-sandwich assay using the electrochemical impedance spectroscopy technique. For this purpose, we designed a signal-up configuration whose linker probes could hybridize with two regions of the target DNA. In this configuration, the presented target DNA would effectively decrease the electron transfer, which would improve the sensitivity of the sensor. Ultimately, we employed gel electrophoresis to further confirm the formation of the proposed super-sandwich structure.
authors.affiliation: Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
authors.forename: Long Yin
authors.initials: LY
authors.lastname: Zhou
authors.forename: Xi Yuan
authors.initials: XY
authors.lastname: Zhang
authors.forename: Gang Lin
authors.initials: GL
authors.lastname: Wang
authors.forename: Xiao Xia
authors.initials: XX
authors.lastname: Jiao
authors.forename: Hong Qun
authors.initials: HQ
authors.lastname: Luo
authors.forename: Nian Bing
authors.initials: NB
authors.lastname: Li
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D058266:Dielectric Spectroscopy / Q000379:methods*; D004563:Electrochemistry / Q000379:methods*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry / Q000235:genetics; D009994:Osmolar Concentration; D013997:Time Factors
chemical_list: D009841:Oligonucleotides; D004247:DNA