title: The influence of electrospun fibre scaffold orientation and nano-hydroxyapatite content on the development of tooth bud stem cells in vitro.
abstract: In stem cell-based dental tissue engineering, the goal is to create tooth-like structures using scaffold materials to guide the dental stem cells. In this study, the effect of fiber alignment and hydroxyapatite content in biodegradable electrospun PLGA scaffolds have been investigated. Fiber orientation of the scaffolds was random or aligned in bundles. For scaffolds with prefabricated orientation, scaffolds were fabricated from PLGA polymer solution containing 0, 10 or 20 % nano-hydroxyapatite. The scaffolds were seeded with porcine cells isolated from tooth buds (dental mesenchymal, dental epithelial, and mixed dental mesenchymal/epithelial cells). Samples were collected at 1, 3 and 6 weeks. Analyses were performed for cell proliferation, ALP activity, and cell morphology. Fiber alignment showed an effect on cell orientation in the first week after cell seeding, but had no long-term effect on cell alignment or organized calcified matrix deposition once the cells reach confluency. Scaffold porosity was sufficient to allow migration of mesenchymal cells. Hydroxyapatite incorporation did not have a positive effect on cell proliferation, especially of epithelial cells, but seemed to promote differentiation. Concluding, scaffold architecture is important to mesenchymal cell morphology, but has no long-term effect on cell alignment or organized ECM deposition. nHA incorporation does have an effect on cell proliferation, differentiation and ECM production, and should be regarded as a bioactive component of dental bioengineered scaffolds.
authors.affiliation: Department of Biomaterials, Radboud University Nijmegen Medical Centre 309 PB, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.
authors.forename: Elisabeth H C
authors.initials: EH
authors.lastname: van Manen
authors.forename: Weibo
authors.initials: W
authors.lastname: Zhang
authors.forename: X Frank
authors.initials: XF
authors.lastname: Walboomers
authors.forename: Betsy
authors.initials: B
authors.lastname: Vazquez
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Na
authors.initials: N
authors.lastname: Yu
authors.forename: Daisy J
authors.initials: DJ
authors.lastname: Spear
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.forename: Pamela C
authors.initials: PC
authors.lastname: Yelick
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials; D017886:Durapatite / Q000032:analysis*; D066298:In Vitro Techniques; D019344:Lactic Acid; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology; D054457:Tissue Scaffolds; D014070:Tooth / Q000166:cytology* / Q000201:enzymology
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D017886:Durapatite; D000469:Alkaline Phosphatase