title: [Protective effect of epigallocatechin-3-gallate on apoptosis of rat cerebellar granule neurons induced by acrylamide].
abstract: OBJECTIVE
  To investigate the protective effect of epigallocatechin-3 -gallate (EGCG) on apoptosis of cerebellar granule neurons (CGNs) induced by acrylamide (ACR).
  
  
  METHODS
  CGNs were cultured with the addition of 5 mmol/L ACR for 24 hours to set up a cell injury model. Prior to ACR treatment, CGNs were treated with different concentrations of EGCG (0, 5, 10, 25, 50, 100 μmol/L) for 48 hours. Neuronal viability was measured with metylthiazdyltetrazolium (MTT). The activity of SOD and the content of MDA were assayed. Hoechst33342 staining was employed to observe morphological changes of the cell nucleus. Reverse transcription-polymerase chain reaction (RT-PCR) was used to measure expression of bcl- 2 mRNA and bax mRNA.
  
  
  RESULTS
  At the concentrations of 10, 25 or 50 μmol/L, EGCG played a protective role against ACRinduced CGN injury. Compared with ACR injured group (no EGCG), EGCG improved the cell viability, enhanced SOD activity, decreased the level of MDA as well as the cell apoptosis ratio (P<0.05). Bcl-2 mRNA expression was increased and bax mRNA expression was reduced (P<0.05). 25 μmol/L EGCG had the largest effect. However, 100 μmol/L EGCG did not have a significantly protective effect.
  
  
  CONCLUSIONS
  EGCG at appropriate concentration has protective effect against the CGNs on apoptosis induced by ACR.
authors.affiliation: First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Liu
authors.forename: Chengmei
authors.initials: C
authors.lastname: Jiang
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhou
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects; D003594:Cytoplasmic Granules; D005260:Female; D008297:Male; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D018696:Neuroprotective Agents / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018696:Neuroprotective Agents; D020106:Acrylamide; D002392:Catechin; C045651:epigallocatechin gallate