title: Identification and characterization of a human IL-10 receptor antagonist.
abstract: Interleukin-10 (IL-10) is an anti-inflammatory cytokine that works through IL-10 receptor alpha subunit- and suppresses immune responses in many infectious diseases such as leishmaniasis as well as in cancer. Therefore, in order to restore the host-protective immune responses in such diseases, an antagonist to this cytokine is a pressing need. Herein, using phage peptide library display, we have identified a dodecameric peptide that functions as an antagonist to human IL-10 receptor in an IL-10-induced STAT3 phosphorylation assay. The peptide antagonist's ability to restore anti-leishmanial function in CD40-activated macrophages was also tested. We observed that the peptide reduced IL-10-induced STAT-3 phosphorylation and enhanced CD40-activated leishmanial functions in macrophages.
authors.affiliation: National Centre for Cell Science, Ganeshkhind, Pune 411007, India.
authors.forename: Mohammed Mumtaz
authors.initials: MM
authors.lastname: Naiyer
authors.forename: Shipra
authors.initials: S
authors.lastname: Saha
authors.forename: Vijayshree
authors.initials: V
authors.lastname: Hemke
authors.forename: Somenath
authors.initials: S
authors.lastname: Roy
authors.forename: Shailza
authors.initials: S
authors.lastname: Singh
authors.forename: Krishnasastry V
authors.initials: KV
authors.lastname: Musti
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Saha
mesh_terms: D000981:Antiprotozoal Agents / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D001667:Binding, Competitive; D019013:CD40 Antigens / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects; D057166:High-Throughput Screening Assays; D006790:Host-Parasite Interactions / Q000187:drug effects / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D007893:Leishmania donovani / Q000187:drug effects* / Q000502:physiology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000469:parasitology; D008958:Models, Molecular; D009842:Oligopeptides / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D019151:Peptide Library; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding; D053701:Receptors, Interleukin-10 / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000981:Antiprotozoal Agents; D019013:CD40 Antigens; D009842:Oligopeptides; D019151:Peptide Library; D053701:Receptors, Interleukin-10; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D016753:Interleukin-10