title: Enantioselective analysis and degradation studies of isocarbophos in soils by chiral liquid chromatography-tandem mass spectrometry.
abstract: An enantioselective method is presented for the determination of isocarbophos in soil by liquid chromatography coupled with tandem mass spectrometry. The pesticide residues in soil samples were extracted with acetonitrile, and complete enantioseparation was obtained on an amylose tris(3,5-dimethylphenylcarbamate) chiral column using acetonitrile/2 mM ammonium acetate solution containing 0.1% formic acid (60:40, v/v) as the mobile phase. The absolute configuration of isocarbophos enantiomers was determined by the combination of experimental and calculated electronic circular dichroism spectra. The method was utilized to investigate the degradation of isocarbophos in soils (Changchun, Hangzhou, and Zhengzhou) under sterilized or native conditions. Isocarbophos enantiomers were configurationally stable in the selected soils, and the pesticide degradation was not enantioselective in the sterilized condition. The degradation behavior of rac-isocarbophos was different under native conditions, with no enantioselectivity in the Changchun soil and with the S-(+)-isocarbophos enriched in the Hangzhou and Zhengzhou soils.
authors.affiliation: Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhang
authors.forename: Xinquan
authors.initials: X
authors.lastname: Wang
authors.forename: Shulin
authors.initials: S
authors.lastname: Zhuang
authors.forename: Nuo
authors.initials: N
authors.lastname: Jin
authors.forename: Xiangyun
authors.initials: X
authors.lastname: Wang
authors.forename: Mingrong
authors.initials: M
authors.lastname: Qian
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Peipei
authors.initials: P
authors.lastname: Qi
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D006207:Half-Life; D007306:Insecticides; D008294:Malathion / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D010573:Pesticide Residues / Q000032:analysis* / Q000737:chemistry; D012987:Soil / Q000032:analysis*; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry
chemical_list: D007306:Insecticides; D010573:Pesticide Residues; D012987:Soil; C549713:isocarbophos; D008294:Malathion