title: In vivo measurement of CBF using ¹⁷O NMR signal of metabolically produced H₂¹⁷O as a perfusion tracer.
abstract: The cerebral metabolic rate of oxygen of small animals can be reliably imaged using the in vivo (17) O magnetic resonance approach at high field. However, a separate measurement is required for imaging the cerebral blood flow in the same animal. In this study, we demonstrate that the (17) O NMR signal of metabolically produced H2 (17) O in the rat brain following an (17) O2 inhalation can serve as a perfusion tracer and its decay rate can be used to determine the absolute values of cerebral blood flow across a wide range of animal conditions. This finding suggests that the in vivo (17) O magnetic resonance approach is capable of imaging both cerebral metabolic rate of oxygen and cerebral blood flow simultaneously and noninvasively; and it provides new utilities for studying the cerebral oxygen metabolism and perfusion commonly associated with brain function and diseases.
authors.affiliation: Department of Radiology, Center for Magnetic Resonance Research, University of Minnesota Medical School, Minneapolis, Minnesota, USA. zhu@cmrr.umn.edu
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Zhu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hannes M
authors.initials: HM
authors.lastname: Wiesner
authors.forename: Kamil
authors.initials: K
authors.lastname: Ugurbil
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000502:physiology; D001921:Brain / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D010092:Oximetry / Q000379:methods*; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption / Q000502:physiology*; D010103:Oxygen Isotopes / Q000493:pharmacokinetics; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010103:Oxygen Isotopes; D019275:Radiopharmaceuticals; D010100:Oxygen