title: [Protein kinase C mediates thrombin-induced monocyte chemoattractant protein-1 release from human lung fibroblasts].
abstract: OBJECTIVE
  To investigate the role of protein kinase C (PKC) in thrombin-induced monocyte chemoattractant protein-1(MCP-1) release by human lung fibroblasts (HLF-1).
  
  
  METHODS
  Cultured human lung fibroblasts HLF-1 were incubated with different concentrations of PKC inhibitors before by thrombin stimulation. MCP-l protein levels in the supernatants were assessed using ELISA, and MCP-1 mRNA levels in the cell lysate were measured by quantitative real-time PCR.
  
  
  RESULTS
  The broad spectrum PKC inhibitors bisindolylmaleimide I and RO-31-8220 obviously inhibited thrombin-induced MCP-l mRNA and protein expressions in HLF-1 cells, whereas Ca(2+)-dependent PKC inhibitor Go 6976 had no such effects.
  
  
  CONCLUSIONS
  Ca(2+)-independent PKC mediates thrombin-induced MCP-1 release in cultured HLF-1 cells.
authors.affiliation: Department of Cell Biology and Genetics, Shantou University Medical College, Shantou University Medical College, Shantou 515041, China. yuanchao.10086@163.com
authors.forename: Chao
authors.initials: C
authors.lastname: Yuan
authors.forename: Mingyan
authors.initials: M
authors.lastname: Xu
authors.forename: Gengzhen
authors.initials: G
authors.lastname: Chen
authors.forename: Yucai
authors.initials: Y
authors.lastname: Fu
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Deng
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D008168:Lung / Q000166:cytology / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D013917:Thrombin / Q000494:pharmacology
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D007211:Indoles; D011493:Protein Kinase C; D013917:Thrombin; C064758:Ro 31-8220