title: [The application of method of flow cytometry in diagnostics of hereditary spherocytosis (eosin-5 maleimid binding test)].
abstract: The technique of binding eosin-5 maleimid fluorescent dye with lysine-430 of first extracellular protein bulge of band 3 of erythrocytes' membranes makes it possible to detect the defects of cytoskeleton of erythrocytes as a biological foundation of pathogenesis of hereditary spherocytosis. The samples of peripheral blood from 125 adult persons and 18 children with established absence of hematologic disorders were analyzed The samples of peripheral blood from 19 patients with verified hereditary spherocytosis were analyzed too. The method of flow cytometry was applied to register the average intensity of fluorescence of eosin-5 maleimid. The decrease of average intensity of fluorescence of eosin-5 maleimid of erythrocytes of patients with hereditary spherocytosis as compared with data from comparison groups was established in all cases.
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Prokhorova
authors.forename: E E
authors.initials: EE
authors.lastname: Zueva
authors.forename: N E
authors.initials: NE
authors.lastname: Sokolova
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004801:Eosine Yellowish-(YS) / Q000031:analogs & derivatives*; D004910:Erythrocyte Membrane / Q000473:pathology; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D008565:Membrane Proteins / Q000097:blood*; D008875:Middle Aged; D013103:Spherocytosis, Hereditary / Q000097:blood*
chemical_list: D005456:Fluorescent Dyes; D008565:Membrane Proteins; C033531:eosin maleimide; D004801:Eosine Yellowish-(YS)