title: Repeated anterior chamber fluorophotometric evaluation of the eyes of ophthalmologically normal dogs.
abstract: OBJECTIVE
  To perform repeated anterior chamber fluorophotometry on both eyes of ophthalmologically normal dogs to measure fluorescein concentrations over a 5-day period and identify any change in the degree of anterior chamber fluorescence over time or difference between eyes.
  
  
  METHODS
  9 healthy adult dogs (18 eyes).
  
  
  METHODS
  Each dog received an IV injection of 10% fluorescein solution, and anterior chamber fluorophotometry was performed 1 hour later on both eyes. This procedure was repeated at the same time each day for 5 consecutive days.
  
  
  RESULTS
  A significant increase in fluorescein concentration was evident in the anterior chamber on day 5 in the right eye and days 2, 3, 4, and 5 in the left eye. There was no significant difference in concentration between the left and the right eyes on any day.
  
  
  CONCLUSIONS
  The increase in ocular fluorescein concentration in the study dogs was unlikely to be of clinical importance and is only pertinent for subsequent research studies. This is a limitation that should be considered when reporting fluorophotometry data as fluorescein concentration or as change in fluorescein concentration from baseline.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: Victoria S
authors.initials: VS
authors.lastname: Miller
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Allbaugh
authors.forename: James K
authors.initials: JK
authors.lastname: Roush
authors.forename: Amy J
authors.initials: AJ
authors.lastname: Rankin
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000378:metabolism*; D018916:Blood-Aqueous Barrier / Q000378:metabolism*; D004285:Dogs / Q000378:metabolism*; D005260:Female; D019793:Fluorescein / Q000493:pharmacokinetics*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics*; D015590:Fluorophotometry / Q000379:methods* / Q000662:veterinary; D008297:Male; D008954:Models, Biological; D012016:Reference Values; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D019793:Fluorescein