title: Triptolide inhibits amyloid-β production and protects neural cells by inhibiting CXCR2 activity.
abstract: Triptolide, a biologically active natural product from Tripterygium wilfordii, protects neurons from inflammation-mediated damage. Our results showed for the first time that triptolide inhibited the expression of CXCR2 and presenilin in a neuroblastoma cell line SHSY5Ysw. Moreover, triptolide potently inhibited amyloid-β1-42 production with IC50 value of 30 pM in HEK293sw cells or 2 nM in SHSY5Ysw cells, respectively. We also demonstrated that triptolide prevented primary cortical neurons from chemokine CXCL1-induced cytotoxicity. Therefore, our study indicates that the neural protective effect of triptolide is largely mediated by inhibiting CXCR2 activity.
authors.affiliation: Department of Pharmacognosy, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Wang
authors.forename: Zi-Qi
authors.initials: ZQ
authors.lastname: Shi
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Xu
authors.forename: Gui-Zhong
authors.initials: GZ
authors.lastname: Xin
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Lian-Wen
authors.initials: LW
authors.lastname: Qi
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D000818:Animals; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004224:Diterpenes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004852:Epoxy Compounds / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010446:Peptide Fragments / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010616:Phenanthrenes / Q000494:pharmacology*; D051381:Rats; D023063:Receptors, Interleukin-8B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D029757:Tripterygium
chemical_list: D016229:Amyloid beta-Peptides; D004224:Diterpenes; D004852:Epoxy Compounds; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D010616:Phenanthrenes; D023063:Receptors, Interleukin-8B; C075222:amyloid beta-protein (1-42); C001899:triptolide