title: Development of oligonucleotide microarrays onto Si-based surfaces via thioether linkage mediated by UV irradiation.
abstract: Selective covalent immobilization of thiolated oligonucleotides onto an epoxy-functionalized silicon-substrate can be achieved via light radiation (365 nm). Following this approach, thiol-modified oligonucleotide probes were covalently attached as microarrays, reaching an immobilization density of 2.5 pmol·cm(-2), with a yield of 53%. The developed method presents the advantages of spatially controlled probe anchoring (by means of using a photomask), direct attachment without using cross-linkers, and short irradiation times (10 min). Hybridization efficiencies up to 65%, with full complementary strands, were reached. The approach was evaluated by scoring single nucleotide polymorphisms with a discrimination ratio around 15. Moreover, sensitive and selective detection of bacterial  Escherichia coli  was demonstrated.
authors.affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Escorihuela
authors.forename: María-José
authors.initials: MJ
authors.lastname: Bañuls
authors.forename: Rosa
authors.initials: R
authors.lastname: Puchades
authors.forename: Ángel
authors.initials: Á
authors.lastname: Maquieira
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D004852:Epoxy Compounds / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012825:Silicon / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry*; D013499:Surface Properties; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D004852:Epoxy Compounds; D013438:Sulfhydryl Compounds; D013440:Sulfides; D012825:Silicon