title: Effects of irradiation on osteoblast-like cells on different titanium surfaces in vitro.
abstract: The aim of this study was to investigate the effects of irradiation on adhesion ability, proliferation, and differentiation of MC3T3-E1 cells on microarc oxidation (MAO) titanium surfaces and polished titanium (PT) surfaces. MC3T3-E1 cells were exposed to a single dose at 2, 4, 6, 8, or 10 Gy using a (60) Co source, with tissue culture polystyrene plates chosen as controls. On all surfaces, irradiation resulted in a dose-dependent decrease in cellular proliferation. At 4 Gy dose, the cell proliferation of cells decreased by 17.8% on MAO and 18.6% on PT surfaces, respectively, compared with nonirradiated controls. Cells exposed to 8 Gy dose showed significant inhibition in collagen secretion and osteogenesis-related genes expression (OSX, COL-Iα1, and OCN). In contrast, irradiation increased cell adhesion to three surfaces dose dependently. It was also demonstrated that cells on MAO surface showed higher adhesion and collagen secretion than on PT surface at different radiation doses. This study revealed the effects of irradiation on osteoblasts in vitro on two titanium surfaces. MAO surface could be used in dental implants in irradiated bone due to enhanced adhesion ability and collagen secretion in osteoblasts.
authors.affiliation: Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Feng
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
authors.forename: Nianbao
authors.initials: N
authors.lastname: Zha
authors.forename: Banglian
authors.initials: B
authors.lastname: Deng
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D049109:Cell Proliferation; D017931:DNA Primers; D015870:Gene Expression; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D010012:Osteogenesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium
chemical_list: D017931:DNA Primers; D014025:Titanium