title: A method for short-term culture of human gastric epithelial cells to study the effects of Helicobacter pylori.
abstract: In vitro studies of Helicobacter pylori pathogenesis mostly rely on the use of tumor-derived cell lines. Although invaluable, tumor cell lines are not representative of the normal cell physiology. Thus, the use of primary gastric epithelial cell cultures provides an important tool for investigating the mechanisms underlying H. pylori infection, as well as for validating the in vitro findings obtained with tumor-derived cell line models. Here we describe a method for isolation and short-term culture of human primary gastric epithelial cells obtained from gastric biopsy specimens, and the use of these cells to evaluate the effect of H. pylori on the junctional adhesion molecule-A protein.
authors.affiliation: IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal.
authors.forename: Marina
authors.initials: M
authors.lastname: Leite
authors.forename: Ceu
authors.initials: C
authors.lastname: Figueiredo
mesh_terms: D015815:Cell Adhesion Molecules / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D005773:Gastroscopy; D016480:Helicobacter pylori / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D007633:Keratins / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D013270:Stomach / Q000166:cytology*; D013997:Time Factors
chemical_list: D015815:Cell Adhesion Molecules; C407243:F11R protein, human; D011956:Receptors, Cell Surface; D007633:Keratins