title: CD22 antigen is broadly expressed on lung cancer cells and is a target for antibody-based therapy.
abstract: Most patients with lung cancer still die from their disease, necessitating additional options to improve treatment. Here, we provide evidence for targeting CD22, a cell adhesion protein known to influence B-cell survival that we found is also widely expressed in lung cancer cells. In characterizing the antitumor activity of an established anti-CD22 monoclonal antibody (mAb), HB22.7, we showed CD22 expression by multiple approaches in various lung cancer subtypes, including 7 of 8 cell lines and a panel of primary patient specimens. HB22.7 displayed in vitro and in vivo cytotoxicity against CD22-positive human lung cancer cells and tumor xenografts. In a model of metastatic lung cancer, HB22.7 inhibited the development of pulmonary metastasis and extended overall survival. The finding that CD22 is expressed on lung cancer cells is significant in revealing a heretofore unknown mechanism of tumorigenesis and metastasis. Our work suggests that anti-CD22 mAbs may be useful for targeted therapy of lung cancer, a malignancy that has few tumor-specific targets.
authors.affiliation: Division of Hematology and Oncology, Department of Internal Medicine, University of California Davis Comprehensive Cancer Center, Sacramento, CA 95817, USA. joseph.tuscano@ucdmc.ucdavis.edu
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Tuscano
authors.forename: Jason
authors.initials: J
authors.lastname: Kato
authors.forename: David
authors.initials: D
authors.lastname: Pearson
authors.forename: Chengyi
authors.initials: C
authors.lastname: Xiong
authors.forename: Laura
authors.initials: L
authors.lastname: Newell
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Ma
authors.forename: David R
authors.initials: DR
authors.lastname: Gandara
authors.forename: Robert T
authors.initials: RT
authors.lastname: O'Donnell
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015152:Blotting, Northern; D015153:Blotting, Western; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D051918:Sialic Acid Binding Ig-like Lectin 2 / Q000096:biosynthesis*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: D000911:Antibodies, Monoclonal; C497867:CD22 protein, human; D034741:RNA, Small Interfering; D051918:Sialic Acid Binding Ig-like Lectin 2