title: Sevoflurane combined with ATP activates caspase-1 and triggers caspase-1-dependent pyroptosis in murine J774 macrophages.
abstract: Sevoflurane is one of the most commonly used volatile anesthetics. Recent studies have shown that sevoflurane plays an important role in modulation of inflammation and immunity. However, little is known about the related molecular mechanisms. This study was designed to investigate the effects and mechanisms of sevoflurane on inflammatory cell death pyroptosis in the murine macrophage cell line J774 cells. Sevoflurane combined with ATP could increase the level of activated caspase-1, pyroptosis, and reactive oxygen species (ROS). Furthermore, treatment of cells with the caspase-1 inhibitor Ac-YVAD-CMK dramatically decreased the percentage of pyroptosis. In addition, inhibition of ROS with N-acetyl-L-cysteine or diphenyleneiodonium significantly reduced the activated levels of caspase-1. These results demonstrated that sevoflurane combined with ATP could activate caspase-1 and trigger caspase-1-dependent pyroptosis through the modulation of ROS production.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, QingChun Road 79, 310003, Hangzhou, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Jin
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Guohao
authors.initials: G
authors.lastname: Xie
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Chen
authors.forename: Shuijin
authors.initials: S
authors.lastname: Wu
authors.forename: Xiangming
authors.initials: X
authors.lastname: Fang
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000818:Animals; D020170:Caspase 1 / Q000378:metabolism*; D061945:Caspase Inhibitors / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects* / Q000502:physiology; D002460:Cell Line; D002470:Cell Survival; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008738:Methyl Ethers / Q000494:pharmacology*; D051379:Mice; D009861:Onium Compounds / Q000494:pharmacology; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism; D000077149:Sevoflurane
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D061945:Caspase Inhibitors; D015853:Cysteine Proteinase Inhibitors; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D008738:Methyl Ethers; C098738:N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone; D009861:Onium Compounds; D010975:Platelet Aggregation Inhibitors; D017382:Reactive Oxygen Species; D000077149:Sevoflurane; C007517:diphenyleneiodonium; D000255:Adenosine Triphosphate; D020170:Caspase 1; D000111:Acetylcysteine