title: Development of a refined tenocyte expansion culture technique for tendon tissue engineering.
abstract: The aim of this study was to efficiently expand less differentiated tenocytes with minimum use of fetal bovine serum (FBS) for tenocyte-based tendon tissue engineering. To achieve this goal, human tenocytes were cultured in different concentrations of FBS and combinations of growth factors PDGF(BB), IGF-1 and bFGF. A number of growth factors were selected that could support tenocyte expansion at reduced differentiated state with minimum FBS usage. Results showed that the expansion of the tenocytes cultured for 14 days with 1% FBS, 50 ng/ml PDGF(BB) and 50 ng/ml bFGF was similar to that cultured in the 10% FBS control group. The tenocytes cultured in the treatment group showed significantly lower collagen synthesis and down-regulation of mRNA expression of tendon differentiation markers. Cell morphology confirmed that tenocytes cultured in the growth factors had reduced collagen fibril formation compared to tenocytes cultured in 10% FBS. Our findings confirm the feasibility of inducing human tenocyte expansion in vitro with the least amount of FBS usage, while controlling their differentiation until required.
authors.affiliation: General Surgery Department, Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin, China; Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Qiu
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Yaonan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Carr
authors.forename: Liwei
authors.initials: L
authors.lastname: Zhu
authors.forename: Zhidao
authors.initials: Z
authors.lastname: Xia
authors.forename: Afsie
authors.initials: A
authors.lastname: Sabokbar
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013710:Tendons / Q000166:cytology*; D023822:Tissue Engineering