title: Efficient decolorization and deproteinization using uniform polymer microspheres in the succinic acid biorefinery from bio-waste cotton (Gossypium hirsutum L.) stalks.
abstract: Bio-waste cotton (Gossypium hirsutum L.) stalks were converted into succinic acid by simultaneous saccharification and fermentation (SSF) using Actinobacillus succinogenes 130Z. After 54 h SSF at 40 °C and pH 7.0, the production of succinic acid was 63 g/L, with 1.17 g/L/h productivity and 64% conversion yield. After SSF, a simple method for the decolorization and deproteinization of crude SSF broth was developed through adsorption tests of polystyrene (PSt) microspheres. Under optimized conditions (5% PSt loading (w/v), pH 4.0, 60 °C and adsorption time of 40 min), the ratios of decolorization, deproteinization and succinic acid loss ratios were 96.6, 84.5 and 4.1%, respectively. The method developed will provide a potential approach for large-scale production of succinic acid from the biomass waste.
authors.affiliation: National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Jiandu
authors.initials: J
authors.lastname: Lei
authors.forename: Rongyue
authors.initials: R
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xing
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Fangling
authors.initials: F
authors.lastname: Gong
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Yan
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Su
authors.forename: Guanghui
authors.initials: G
authors.lastname: Ma
mesh_terms: D000188:Actinobacillus / Q000378:metabolism; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism; D002606:Charcoal; D003116:Color; D005285:Fermentation; D003368:Gossypium / Q000737:chemistry*; D006868:Hydrolysis; D008863:Microspheres; D010940:Plant Proteins / Q000302:isolation & purification; D011137:Polystyrenes / Q000737:chemistry*; D019802:Succinic Acid / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis*
chemical_list: D010940:Plant Proteins; D011137:Polystyrenes; D014866:Waste Products; D002606:Charcoal; D019802:Succinic Acid; D002480:Cellulase