title: Bryostatin-1 promotes long-term potentiation via activation of PKCα and PKCε in the hippocampus.
abstract: Activation of protein kinase C (PKC) by bryostatin-1 affects various functions of the central nervous system. We explored whether bryostatin-1 influenced synaptic plasticity via a process involving PKC. Our purpose was to examine whether bryostatin-1 affected the induction of hippocampal long-term potentiation (LTP) in Schaffer-collateral fibers (CA1 fibers) of the hippocampus, and/or influenced the intracellular Ca(2+) level of hippocampal neurons. We also determined the PKC isoforms involved in these processes. We found that bryostatin-1 strongly facilitated LTP induction, in a dose-dependent manner, upon single-theta burst stimulation (TBS). Further, intracellular Ca(2+) levels also increased with increasing concentration of bryostatin-1. The facilitative effects of bryostatin-1 in terms of LTP induction and enhancement of intracellular Ca(2+) levels were blocked by specific inhibitors of PKCα and PKCε, but not of PKCδ. Our results suggest that bryostatin-1 is involved in neuronal functioning and facilitates induction of LTP via activation of PKCα and/or PKCε.
authors.affiliation: Department of Physiology and Biophysics, School of Medicine, Eulji University, Daejeon 301-746, Republic of Korea.
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: S H
authors.initials: SH
authors.lastname: Han
authors.forename: H Y
authors.initials: HY
authors.lastname: Quan
authors.forename: Y-J
authors.initials: YJ
authors.lastname: Jung
authors.forename: J
authors.initials: J
authors.lastname: An
authors.forename: P
authors.initials: P
authors.lastname: Kang
authors.forename: J-B
authors.initials: JB
authors.lastname: Park
authors.forename: B-J
authors.initials: BJ
authors.lastname: Yoon
authors.forename: G H
authors.initials: GH
authors.lastname: Seol
authors.forename: S S
authors.initials: SS
authors.lastname: Min
mesh_terms: D000818:Animals; D054713:Bryostatins / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003627:Data Interpretation, Statistical; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D055724:Electrophysiological Phenomena; D004789:Enzyme Activation / Q000187:drug effects; D020536:Enzyme Activators / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology*; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism; D017774:Long-Term Potentiation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D051744:Protein Kinase C-epsilon / Q000378:metabolism*
chemical_list: D054713:Bryostatins; D020536:Enzyme Activators; D007527:Isoenzymes; C046785:bryostatin 1; D051571:Protein Kinase C-alpha; D051744:Protein Kinase C-epsilon; D002118:Calcium