title: Sperm DNA fragmentation analysis using the TUNEL assay.
abstract: Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick end labeling or the TUNEL assay is an important technique in the assessment of DNA damage. Semen samples are routinely assessed microscopically to assess their fertilization ability. In addition to routine semen analysis, the use of the TUNEL assay can provide information on the level of DNA damage present within a sample. This chapter provides a practical walk-through guide aimed at directing a researcher or a clinical facility interested in setting up and using TUNEL and flow cytometry or fluorescence microscopy for sperm DNA analysis.
authors.affiliation: Center for Reproductive Medicine, Glickman Urological & Kidney Institute, Cleveland Clinic, Cleveland, OH, USA. sharmar@ccf.org
authors.forename: Rakesh
authors.initials: R
authors.lastname: Sharma
authors.forename: Jayson
authors.initials: J
authors.lastname: Masaki
authors.forename: Ashok
authors.initials: A
authors.lastname: Agarwal
mesh_terms: D053938:DNA Fragmentation; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling / Q000379:methods*; D007248:Infertility, Male / Q000175:diagnosis / Q000235:genetics; D008297:Male; D008856:Microscopy, Fluorescence; D055101:Semen Analysis / Q000379:methods; D013094:Spermatozoa / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods