title: Manual methods for sperm motility assessment.
abstract: Progressive motility is a vital functional characteristic of ejaculated human spermatozoa that governs their ability to penetrate into, and migrate through, both cervical mucus and the oocyte vestments, and ultimately fertilize the oocyte. A detailed protocol, based on traditional manual/visual methods, is provided for performing an accurate four-category differential count including the reliable identification of rapid progressive (grade "a") spermatozoa-the most biologically, and hence clinically, important subpopulation. Thorough prior training and the use of a microscope fitted with a heated stage are both essential requirements for achieving accuracy and an acceptable uncertainty of measurement of no more than ±10%.
authors.affiliation: Oozoa Biomedical Inc, West Vancouver, BC, Canada. david@oozoa.com
authors.forename: David
authors.initials: D
authors.lastname: Mortimer
authors.forename: Sharon T
authors.initials: ST
authors.lastname: Mortimer
mesh_terms: D006801:Humans; D008297:Male; D055101:Semen Analysis / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*