title: Analysis of the rat hypothalamus proteome by data-independent label-free LC-MS/MS.
abstract: Studies of neuronal, endocrine, and metabolic disorders would be facilitated by characterization of the hypothalamus proteome. Protein extracts prepared from 16 whole rat hypothalami were measured by data-independent label-free nano LC-MS/MS. Peptide features were detected, aligned, and searched against a rat Swiss-Prot database using ProteinLynx Global Server v.2.5. The final combined dataset comprised 21 455 peptides, corresponding to 622 unique proteins, each identified by a minimum of two distinct peptides. The majority of the proteins (69%) were cytosolic, and 16% were membrane proteins. Important proteins involved in neurological and synaptic function were identified including several members of the Ras-related protein family and proteins involved in glutamate biosynthesis.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
authors.forename: Viktoria
authors.initials: V
authors.lastname: Stelzhammer
authors.forename: Bob
authors.initials: B
authors.lastname: Amess
authors.forename: Daniel
authors.initials: D
authors.lastname: Martins-de-Souza
authors.forename: Yishai
authors.initials: Y
authors.lastname: Levin
authors.forename: Susan E
authors.initials: SE
authors.lastname: Ozanne
authors.forename: Malgorzata S
authors.initials: MS
authors.lastname: Martin-Gronert
authors.forename: Sebastian
authors.initials: S
authors.lastname: Urday
authors.forename: Sabine
authors.initials: S
authors.lastname: Bahn
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D030562:Databases, Protein; D007031:Hypothalamus / Q000737:chemistry* / Q000378:metabolism; D007526:Isoelectric Point; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000737:chemistry; D010446:Peptide Fragments / Q000032:analysis; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D015415:Biomarkers; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D020543:Proteome