title: Development of FPV140 antigen-specific ELISA differentiating fowlpox virus isolates from all other viral pathogens of avian origin.
abstract: The FPV140 gene encodes an envelope protein of fowlpox virus (FPV). In this study, the FPV140 gene of FPV Chinese isolate HH2008 was cloned and the comparison of its sequence with other FPV isolates showed it to be highly conserved across all FPV isolates. A recombinant plasmid pET-FPV140 carrying FPV140 gene was constructed and transformed into Escherichia coli. The optimal expression condition for the FPV140 gene was developed and purified FPV140 recombinant protein was used to produce rabbit polyclonal antibody. An indirect ELISA using this anti-FPV140 polyclonal antibody was capable of distinguishing avian FPV isolates from other common avian pathogens such as mycoplasma gallisepticum, infectious laryngotracheitis virus, avian influenza virus, infectious bursal disease virus, and avian infectious bronchitis virus. This ELISA will serve as a useful diagnostic tool for the detection of FPV in clinical samples.
authors.affiliation: Northeast Agricultural University, Xiangfang District, Harbin, China
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Hong
authors.forename: Y
authors.initials: Y
authors.lastname: Ren
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: C
authors.initials: C
authors.lastname: He
authors.forename: X
authors.initials: X
authors.lastname: Ren
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D001715:Bird Diseases / Q000175:diagnosis / Q000821:virology*; D001717:Birds; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005586:Fowlpox / Q000235:genetics / Q000378:metabolism*; D015967:Gene Expression Regulation, Viral; D010802:Phylogeny; D011817:Rabbits; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000914:Antibodies, Viral; D014759:Viral Envelope Proteins