title: Quantification of sporozoite invasion, migration, and development by microscopy and flow cytometry.
abstract: There is an important role for in vitro assays to better understand the initial steps of malaria infection. In this section, we describe both microscopy-based and flow cytometry-based sporozoite invasion, migration and development assays with the rodent malaria parasites, Plasmodium berghei and Plasmodium yoelii, and the human malaria parasite, Plasmodium falciparum.
authors.affiliation: Department of Molecular Microbiology and Immunology, John Hopkins Bloomberg School of Public Health, Baltimore, MD, USA. psinnis@jhsph.edu
authors.forename: Photini
authors.initials: P
authors.lastname: Sinnis
authors.forename: Patricia
authors.initials: P
authors.lastname: De La Vega
authors.forename: Alida
authors.initials: A
authors.lastname: Coppi
authors.forename: Urszula
authors.initials: U
authors.lastname: Krzych
authors.forename: Maria M
authors.initials: MM
authors.lastname: Mota
mesh_terms: D000818:Animals; D000852:Anopheles / Q000469:parasitology; D018929:Cell Culture Techniques; D002460:Cell Line; D002465:Cell Movement; D005434:Flow Cytometry / Q000379:methods*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000469:parasitology* / Q000473:pathology; D006801:Humans; D008288:Malaria / Q000469:parasitology; D051379:Mice; D008853:Microscopy / Q000379:methods*; D010961:Plasmodium / Q000254:growth & development*; D034101:Sporozoites / Q000378:metabolism*