title: Cloning of cDNAs and molecular characterisation of C-type lectin-like proteins from snake venoms.
abstract: C-type lectin-like proteins (CTLPs) isolated from snake venoms are the largest and most complex non-mammalian vertebrate C-type lectin-like domain family. In the present study, we simultaneously amplified four cDNAs encoding different types of CTLP subunits from the venoms of two different species of snakes by RT-PCR with a single sense primer and a nested universal primer - two CTLP subunit-encoding cDNAs were cloned from Deinagkistrodon acutus venom and two from Agkistrodon halys Pallas venom. All four cloned CTLP subunits exhibited typical motifs in their corresponding domain regions but with relatively-low sequence similarities to each other. Compared with previously-published CTLPs, the four cloned CTLPs subunits showed slight variations in the calcium-binding sites and the disulphide bonding patterns. To our knowledge, these data constitute the first example of co-expression of CTLP platelet glycoprotein Ib-binding subunits and coagulation factors in Agkistrodon halys Pallas venom.
authors.affiliation: Molecular Therapeutics Research, School of Pharmacy, Medical Biology Centre, McClay Research Centre, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK. jfeng05@qub.ac.uk
authors.forename: Jian
authors.initials: J
authors.lastname: Feng
authors.forename: Tianbao
authors.initials: T
authors.lastname: Chen
authors.forename: Mei
authors.initials: M
authors.lastname: Zhou
authors.forename: Chris
authors.initials: C
authors.lastname: Shaw
mesh_terms: D017836:Agkistrodon; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003435:Crotalid Venoms / Q000737:chemistry*; D017931:DNA Primers; D037181:Lectins, C-Type / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: C031442:Agkistrodon venoms; D003435:Crotalid Venoms; D017931:DNA Primers; D037181:Lectins, C-Type