title: [Determination of six metal elements in animal samples by flame atomic absorption spectrometry with wet digestion].
abstract: The contents of 6 metal elements (Ca, Mg, K, Na, Cu and Fe ) were determined in serum, urine, feces and different tissue and organs (heart, kidney, stomach, liver, spleen, intestine and lung) of Wistar rats and mice by flame atomic absorption spectrometry (FAAS) with wet digestion. The samples were digested by the mixture of HNO3 and HClO4 (4 : 1, V/V) at 120 degrees C, the correlation coefficient for the standard curves was 0.999 4-0.999 8, the relative standard deviation (RSD) was from 0.33% to 4.52%, and the recovery for the samples was from 97.7% to 104.2%, meeting the demand of elements content determination in biological samples. The assay method for the determination of the 6 metal elements in animal samples established in this study has the advantages of easy operation, high sensitivity, less sample requiring and accurate results, and the method can be widely used in the determination of various metal elements in biological samples.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. lmj1985@163.com
authors.forename: Ming-Jia
authors.initials: MJ
authors.lastname: Liu
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Zhou
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D008670:Metals / Q000097:blood / Q000652:urine; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D013054:Spectrophotometry, Atomic; D014018:Tissue Distribution
chemical_list: D008670:Metals