title: [Progress in isolation and purification of porcine islets].
abstract: OBJECTIVE
  To review the common methods of isolation and purification of porcine islets and research progress.
  
  
  METHODS
  Domestic and abroad literature concerning the isolation and purification of porcine islets was reviewed and analyzed thoroughly.
  
  
  RESULTS
  The efficacy of the isolation and purification depends on the selection of donor, the procurement and cryopreservation of high-quality donor pancreas, and the selection and improvement of the operation.
  
  
  CONCLUSIONS
  The shortage of transplanted islets could be resolved by the establishment of standardized and optimal process, which may also promote the development of porcine islet xenograft.
authors.affiliation: Department of Hepatobiliary, First Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an Shaanxi 710061, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhu
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D017364:Collagenases / Q000008:administration & dosage; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods; D009926:Organ Preservation / Q000379:methods*; D010179:Pancreas / Q000601:surgery; D011786:Quality Control; D013552:Swine; D014183:Transplantation, Heterologous
chemical_list: D017364:Collagenases