title: Clinicopathological analysis of a composite lymphoma containing both T- and B-cell lymphomas.
abstract: Composite lymphomas (CLs) have been reported in 1-4.7% of all lymphomas, however, CLs containing both T- and B-cell lymphomas (CTBLs) are very rare. Here, we examined the clinical and pathological features of 29 CTBLs. These CTBLs included 21 patients with angioimmunoblastic T-cell lymphoma (AITL) and diffuse large B-cell lymphoma (DLBCL), two with adult T-cell leukemia/lymphoma and DLBCL, one with AITL and Follicular lymphoma, one with Lennert lymphoma and DLBCL, one with subcutaneous panniculitis-like T-cell lymphoma and DLBCL, one with peripheral T-cell lymphoma (PTCL) and DLBCL, one with cutaneous T-cell lymphoma and DLBCL, and one with PTCL and chronic lymphocytic leukemia. Eighteen of 27 patients (67%) were shown to be Epstein-Barr virus (EBV)-encoded RNA-positive in their B-cell lymphoma component. T-cell and B-cell clonality were confirmed by flow cytometry, Southern blot analysis, and/or polymerase chain reaction (PCR). Using Southern blot analysis, clonal immunoglobulin heavy chain (IgH) and T-cell receptor (TCR) rearrangements were detected in 11 of 21 and 15 of 24 cases, respectively. Using PCR analysis, clonal IgH and TCR rearrangements were detected in 7 of 8 and 7 of 7 Southern blot-negative cases, respectively. Our results suggested that PCR analysis was useful in diagnosing CTBL.
authors.affiliation: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Suefuji
authors.forename: Daisuke
authors.initials: D
authors.lastname: Niino
authors.forename: Fumiko
authors.initials: F
authors.lastname: Arakawa
authors.forename: Kennosuke
authors.initials: K
authors.lastname: Karube
authors.forename: Yoshizo
authors.initials: Y
authors.lastname: Kimura
authors.forename: Junichi
authors.initials: J
authors.lastname: Kiyasu
authors.forename: Masanori
authors.initials: M
authors.lastname: Takeuchi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Miyoshi
authors.forename: Maki
authors.initials: M
authors.lastname: Yoshida
authors.forename: Ayako
authors.initials: A
authors.lastname: Ichikawa
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Sugita
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohshima
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015139:Blotting, Southern; D058617:Composite Lymphoma / Q000235:genetics / Q000473:pathology*; D005260:Female; D005500:Follow-Up Studies; D015322:Gene Rearrangement, B-Lymphocyte; D015329:Gene Rearrangement, T-Lymphocyte; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007564:Japan; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000473:pathology*; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011379:Prognosis; D012367:RNA, Viral / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics
chemical_list: D007143:Immunoglobulin Heavy Chains; D012367:RNA, Viral; D011948:Receptors, Antigen, T-Cell