title: Altered cellular membrane fluidity levels and lipid peroxidation during experimental pancreas transplantation.
abstract: Although the pathogenesis of ischemia reperfusion (IR) injury is based on complex mechanisms, free radicals play a central role. We evaluated membrane fluidity and lipid peroxidation during pancreas transplantation (PT) performed in 12 pigs (six donors and six recipients). Fluidity was measured by fluorescence spectroscopy, and malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA) concentrations were used as an index of lipid oxidation. Pancreatic tissues were collected as follows: (A) donor, immediately before vascular clamping; (B) graft, following perfusion lavage with University of Wisconsin preservation fluid; (C) graft, after 16 h of cold ischemia; and (D) recipient, 30 min vascular postreperfusion. Fluidity and MDA and 4-HDA concentrations were similar in cases A, B, and C. However, there was significant membrane rigidity and increased lipid peroxidation after reperfusion (D). These findings suggest that reperfusion exaggerates oxidative damage and may account for the rigidity in the membranes of allografts during PT.
authors.affiliation: Department of Surgery, Gynaecology and Obstetrics, University of Zaragoza, Zaragoza, Spain.
authors.forename: F A
authors.initials: FA
authors.lastname: García-Gil
authors.forename: C D
authors.initials: CD
authors.lastname: Albendea
authors.forename: L
authors.initials: L
authors.lastname: López-Pingarrón
authors.forename: P
authors.initials: P
authors.lastname: Royo-Dachary
authors.forename: J
authors.initials: J
authors.lastname: Martínez-Guillén
authors.forename: E
authors.initials: E
authors.lastname: Piedrafita
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Díez
authors.forename: J
authors.initials: J
authors.lastname: Soria
authors.forename: J J
authors.initials: JJ
authors.lastname: García
mesh_terms: D000818:Animals; D005260:Female; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D008560:Membrane Fluidity; D008856:Microscopy, Fluorescence / Q000379:methods; D010179:Pancreas / Q000378:metabolism* / Q000473:pathology; D016035:Pancreas Transplantation; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D013552:Swine; D014184:Transplantation, Homologous
chemical_list: D008315:Malondialdehyde