title: Identification and distribution of high-abundance proteins in the octopus spring microbial mat community.
abstract: A shotgun metaproteomics approach was employed to identify proteins in a hot spring microbial mat community. We identified 202 proteins encompassing 19 known functions from 12 known phyla. Importantly, we identified two key enzymes involved in the 3-hydroxypropionate CO(2) fixation pathway in uncultivated Roseiflexus spp., which are known photoheterotrophs.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
authors.forename: Courtney S
authors.initials: CS
authors.lastname: Schaffert
authors.forename: Christian G
authors.initials: CG
authors.lastname: Klatt
authors.forename: David M
authors.initials: DM
authors.lastname: Ward
authors.forename: Mark
authors.initials: M
authors.lastname: Pauley
authors.forename: Laurey
authors.initials: L
authors.lastname: Steinke
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D057486:Carbon Cycle; D002245:Carbon Dioxide / Q000378:metabolism; D041862:Chloroflexi / Q000201:enzymology; D002853:Chromatography, Liquid; D019015:Geologic Sediments / Q000382:microbiology*; D045482:Hot Springs / Q000382:microbiology*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D002245:Carbon Dioxide