title: Site-selective chemistry and the attachment of peptides to the surface of a microelectrode array.
abstract: Peptides have been site-selectively placed on microelectrode arrays with the use of both thiol-based conjugate additions and Cu(I)-coupling reactions between thiols and aryl halides. The conjugate addition reactions used both acrylate and maleimide Michael acceptors. Of the two methods, the Cu(I)-coupling reactions proved far superior because of their irreversibility. Surfaces constructed with the conjugate addition chemistry were not stable at neutral pHs, especially the surface using the maleimide acceptor. Once a peptide was placed onto the array, it could be monitored in "real-time" for its interactions with a biological receptor.
authors.affiliation: Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.
authors.forename: Melissae Stuart
authors.initials: MS
authors.lastname: Fellet
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Bartels
authors.forename: Bo
authors.initials: B
authors.lastname: Bi
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Moeller
mesh_terms: D000179:Acrylates / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004867:Equipment Design; D008301:Maleimides / Q000737:chemistry; D008839:Microelectrodes; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D040081:Protein Array Analysis / Q000295:instrumentation*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000179:Acrylates; D008301:Maleimides; D009842:Oligopeptides; D010455:Peptides; D013438:Sulfhydryl Compounds; C043592:maleimide; D003300:Copper; C047981:arginyl-glycyl-aspartic acid; C036658:acrylic acid