title: [Ri plasmid transformation and metabolism of ginseng products on HepG2 cells].
abstract: OBJECTIVE
  To study the Ri plasmid transformation and metabolism of ginseng products on HepG2 cells.
  
  
  METHODS
  The inhibitory effect of ginseng saponin on hepatoma HepG2 cells was studied. The hairy root-induced conditions were screened by orthogonal experimental design. The culture conditions were determined through hairy root biomass accumulation and saponin content. The effect of ginsenoside on HepG2 cells was determined by MTT assay.
  
  
  CONCLUSIONS
  The optimal ginseng hairy root inducing conditions: A = 0.6, infection time of 10 min, pre-incubation time for the 3 d. The best culture conditions: MS medium, pH 6.1, 24 degrees C. At those conditions the hairy root bio-accumulation and saponin content were higher. The results of ginseng saponins on the inhibitory effect of HepG2 cells showed that inhibition of ginseng saponins on HepG2 was the concentration positively related.
authors.affiliation: Jilin Agricultural University, Bioreactor Engineering and Drug Development Research Center of Ministry of Education, Changchun 130118, China. xiziyang512@163.com
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Tianhang
authors.initials: T
authors.lastname: Li
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Guo
authors.forename: Lili
authors.initials: L
authors.lastname: Guan
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D005818:Genetic Engineering / Q000379:methods*; D056945:Hep G2 Cells; D006801:Humans; D005894:Panax / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics*; D012503:Saponins / Q000032:analysis / Q000494:pharmacology; D014170:Transformation, Genetic
chemical_list: D000970:Antineoplastic Agents; D004365:Drugs, Chinese Herbal; D012503:Saponins