title: [Research on cationic vector-mediated RNAi].
abstract: In order to study the efficiency of small interfering RNA (siRNA) transfer mediated by cationic liposome, we used luciferase siRNA to evaluate the gene silencing activity in the Hep-2 cells, which were stably transduced with a luciferase gene. The pDNA transfection was studied, and siRNA arrearage assay was conducted to determine the capability of cationic liposome with siRNA. Different concentrations of siRNA was used to silence luciferase gene' activity, and then the result was examined by microplate reader. Cell viability was analyzed after transfection by MTT assay. The results suggested that Lipofectamine 2000 could transfer the pDNA efficiently, and have strong binding capacity with siRNA. The silencing efficiency of luciferase was obtained with low concentration of siRNA. The cell viability was influenced by RNA interference (RNAi) very slightly, but the cell survival rate decreased with the increase of siRNA concentrations. It was well concluded that by optimizing the experimental conditions, cationic liposome can transfer low concentration siRNA to silence target gene's activity efficiently.
authors.affiliation: College of Life Science, Liaoning Normal University, Dalian 116029, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.forename: Huiqin
authors.initials: H
authors.lastname: Ding
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Shaohui
authors.initials: S
authors.lastname: Cui
authors.forename: Yinan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Wenshi
authors.initials: W
authors.lastname: Jin
authors.forename: Shubiao
authors.initials: S
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Jin
mesh_terms: D002412:Cations / Q000737:chemistry*; D004337:Drug Carriers; D005822:Genetic Vectors; D056945:Hep G2 Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008156:Luciferases / Q000235:genetics; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D002412:Cations; D004337:Drug Carriers; D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; D034741:RNA, Small Interfering; D008156:Luciferases