title: Fructose rich diet-induced high plasminogen activator inhibitor-1 (PAI-1) production in the adult female rat: protective effect of progesterone.
abstract: The effect of progesterone (P4) on fructose rich diet (FRD) intake-induced metabolic, endocrine and parametrial adipose tissue (PMAT) dysfunctions was studied in the adult female rat. Sixty day-old rats were i.m. treated with oil alone (control, CT) or containing P4 (12 mg/kg). Rats ate Purina chow-diet ad libitum throughout the entire experiment and, between 100 and 120 days of age drank ad libitum tap water alone (normal diet; CT-ND and P4-ND) or containing fructose (10% w/v; CT-FRD and P4-FRD). At age 120 days, animals were subjected to a glucose tolerance test or decapitated. Plasma concentrations of various biomarkers and PMAT gene abundance were monitored. P4-ND (vs. CT-ND) rats showed elevated circulating levels of lipids. CT-FRD rats displayed high (vs. CT-ND) plasma concentrations of lipids, leptin, adiponectin and plasminogen activator inhibitor-1 (PAI-1). Lipidemia and adiponectinemia were high (vs. P4-ND) in P4-FRD rats. Although P4 failed to prevent FRD-induced hyperleptinemia, it was fully protective on FRD-enhanced plasma PAI-1 levels. PMAT leptin and adiponectin mRNAs were high in CT-FRD and P4-FRD rats. While FRD enhanced PMAT PAI-1 mRNA abundance in CT rats, this effect was absent in P4 rats. Our study supports that a preceding P4-enriched milieu prevented the enhanced prothrombotic risk induced by FRD-elicited high PAI-1 production.
authors.affiliation: Neuroendocrine Unit, IMBICE (CONICET-CICPBA), 1900 La Plata, Argentina. dcastrogiovanni@imbice.org.ar
authors.forename: Daniel
authors.initials: D
authors.lastname: Castrogiovanni
authors.forename: Ana
authors.initials: A
authors.lastname: Alzamendi
authors.forename: Luisina
authors.initials: L
authors.lastname: Ongaro
authors.forename: Andrés
authors.initials: A
authors.lastname: Giovambattista
authors.forename: Rolf C
authors.initials: RC
authors.lastname: Gaillard
authors.forename: Eduardo
authors.initials: E
authors.lastname: Spinedi
mesh_terms: D000818:Animals; D001786:Blood Glucose; D002784:Cholesterol / Q000097:blood; D003345:Corticosterone / Q000097:blood; D004032:Diet / Q000009:adverse effects*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005632:Fructose / Q000737:chemistry / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000097:blood; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D020738:Leptin; D017395:Plasminogen Activator Inhibitor 1; D014280:Triglycerides; D005632:Fructose; D013739:Testosterone; D011374:Progesterone; D002784:Cholesterol; D003345:Corticosterone