title: An antioxidant exopolysaccharide devoid of pro-oxidant activity produced by the soil bacterium Bordetella sp. B4.
abstract: An exopolysaccharide (EPS) with a molecular weight of 230 kDa, was isolated from Bordetella sp. B4. The EPS was identified as linear alpha-1,6-(6-methyl)-glucan with N-acetyl-D-glucosamine branches at alpha-1, 4-linkages by IR and NMR spectroscopy. The free radical scavenging capacities of EPS on 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(+)), H(2)O(2), -OH and lipid peroxidation were 2-, 86-, 134- and 18-fold higher than that of ascorbic acid, respectively. Compared with ascorbic acid, the EPS was more effective in preventing DNA and protein from free radical damage induced by 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH). More significantly, the EPS did not degrade DNA and protein by the pro-oxidant effect in the presence of copper ions and H(2)O(2). Furthermore, EPS could protect human umbilical vein endothelium cells (HUVECs) from high glucose-mediated damage. The production of EPS reached 10.2 g/L in the fermentation medium containing 3.0 g/L cholesterol, suggesting that Bordetella sp. B4 was a potential producer of antioxidant EPS.
authors.affiliation: Department of Center Laboratory, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Lin
authors.forename: Jinglei
authors.initials: J
authors.lastname: Liu
authors.forename: Yibo
authors.initials: Y
authors.lastname: Hu
authors.forename: Xin
authors.initials: X
authors.lastname: Song
authors.forename: Yueran
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001884:Bordetella / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000494:pharmacology*; D012988:Soil Microbiology; D013055:Spectrophotometry, Infrared
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D004247:DNA