title: Efficacy of an antiviral compound to inhibit replication of multiple pestivirus species.
abstract: Pestiviruses are economically important pathogens of livestock. An aromatic cationic compound (DB772) has previously been shown to inhibit bovine viral diarrhea virus (BVDV) type 1 in vitro at concentrations lacking cytotoxic side effects. The aim of this study was to determine the scope of antiviral activity of DB772 among diverse pestiviruses. Isolates of BVDV 2, border disease virus (BDV), HoBi virus, pronghorn virus and Bungowannah virus were tested for in vitro susceptibility to DB772 by incubating infected cells in medium containing 0, 0.006, 0.01, 0.02, 0.05, 0.1, 0.2, 0.39, 0.78, 1.56, 3.125, 6.25, 12.5 or 25μM DB772. The samples were assayed for the presence of virus by virus isolation and titration (BDV and BVDV 2) or PCR (HoBi, pronghorn and Bungowannah viruses). Cytotoxicity of the compound was assayed for each cell type. Complete inhibition of BVDV 2, BDV, and Pronghorn virus was detected when DB772 was included in the culture media at concentrations of 0.20μM and higher. In two of three tests, a concentration of 0.05μM DB772 was sufficient to completely inhibit HoBi virus replication. Bungowannah virus was completely inhibited at a concentration of 0.01μM DB772. Thus, DB772 effectively inhibits all pestiviruses studied at concentrations >0.20μM. As cytotoxicity is not evident at these concentrations, this antiviral compound potentially represents an effective preventative or therapeutic for diverse pestiviruses.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, 1500 Wire Rd., Auburn University, AL 36849-5522, USA. bwn0001@auburn.edu
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Newcomer
authors.forename: M Shonda
authors.initials: MS
authors.lastname: Marley
authors.forename: Julia F
authors.initials: JF
authors.lastname: Ridpath
authors.forename: John D
authors.initials: JD
authors.lastname: Neill
authors.forename: David W
authors.initials: DW
authors.lastname: Boykin
authors.forename: Arvind
authors.initials: A
authors.lastname: Kumar
authors.forename: M Daniel
authors.initials: MD
authors.lastname: Givens
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology* / Q000633:toxicity; D001562:Benzimidazoles / Q000494:pharmacology* / Q000633:toxicity; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005663:Furans / Q000494:pharmacology* / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D010576:Pestivirus / Q000187:drug effects* / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects*
chemical_list: C571880:2-(2-benzimidazolyl)-5-(4-(2-imidazolino)phenyl)furan; D000998:Antiviral Agents; D001562:Benzimidazoles; D005663:Furans