title: Influence of metal ions on bioremediation activity of protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2.
abstract: The aim of this paper was to describe the effect of various metal ions on the activity of protocatechuate 3,4-dioxygenase from Stenotrophomonas maltophilia KB2. We also compared activity of different dioxygenases isolated from this strain, in the presence of metal ions, after induction by various aromatic compounds. S. maltophilia KB2 degraded 13 mM 3,4-dihydroxybenzoate, 10 mM benzoic acid and 12 mM phenol within 24 h of incubation. In the presence of dihydroxybenzoate and benzoate, the activity of protocatechuate 3,4-dioxygenase and catechol 1,2-dioxygenase was observed. Although Fe(3+), Cu(2+), Zn(2+), Co(2+), Al(3+), Cd(2+), Ni(2+) and Mn(2+) ions caused 20-80 % inhibition of protocatechuate 3,4-dioxygenase activity, the above-mentioned metal ions (with the exception of Ni(2+)) inhibited catechol 1,2-dioxygenase to a lesser extent or even activate the enzyme. Retaining activity of at least one of three dioxygenases from strain KB2 in the presence of metal ions makes it an ideal bacterium for bioremediation of contaminated areas.
authors.affiliation: Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, Poland. urszula.guzik@us.edu.pl
authors.forename: Urszula
authors.initials: U
authors.lastname: Guzik
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Hupert-Kocurek
authors.forename: Karina
authors.initials: K
authors.lastname: Sałek
authors.forename: Danuta
authors.initials: D
authors.lastname: Wojcieszyńska
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019817:Benzoic Acid / Q000378:metabolism; D001673:Biodegradation, Environmental; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism; D007700:Kinetics; D008670:Metals / Q000378:metabolism*; D011520:Protocatechuate-3,4-Dioxygenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D020615:Stenotrophomonas maltophilia / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: C520290:3,4-dihydroxybenzoate; D001426:Bacterial Proteins; D062385:Hydroxybenzoates; D008670:Metals; D012722:Sewage; D019817:Benzoic Acid; D049308:Dioxygenases; D011520:Protocatechuate-3,4-Dioxygenase