title: An efficient expression and purification strategy for the production of S100 proteins in Escherichia coli.
abstract: S100 proteins belong to a family of small, acidic, EF-hand Ca ( 2+) -binding proteins and have been found to exert both intracellular and extracellular functions in regulation of Ca ( 2+)  homeostasis, cytoskeletal dynamics, cell cycle, motility and differentiation. As a result, they have been widely investigated for their association with diseases, such as, neurological diseases, cardiomyopathy, neoplasias and inflammatory diseases. To facilitate further studies of S100 proteins, we reported a simple and efficient method for the expression and purification of human S100A4 and S100A11 proteins in Escherichia coli. Since S100 proteins share many common physical and chemical characteristics, we expect that this approach can be extended to the production of most S100 proteins.
authors.affiliation: Shanghai Municipality Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology; Shanghai Jiao Tong University, Shanghai, P.R. China.
authors.forename: Honglin
authors.initials: H
authors.lastname: He
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Wen
authors.initials: W
authors.lastname: Guan
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
mesh_terms: D002852:Chromatography, Ion Exchange / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; C077591:S100A4 protein, human; C072337:S100A11 protein, human