title: Detection of five rash-associated viruses using multiplex real-time PCR during 2006-2011.
abstract: Many viruses have been reported to be associated with rash development. Multiplex real-time  PCR was used to investigate the presence of 5 viruses associated with rashes: measles virus (MV), rubella  virus (RV), human parvovirus B19 (PVB19), human herpes virus 6 (HHV-6), and HHV-7. A total of 187 clinical  specimens from 169 patients with erythema were collected between January 2006 and December 2011.  Virus-positive specimens were as follows: MV (n = 23), PVB19 (n = 8), RV (n = 2), HHV-6 (n = 5), HHV-7  (n = 1), MV and PVB19 (n = 1), and HHV-6 and HHV-7 (n = 1). All of the MV-positive specimens were collected  in 2007 and the strains whose sequence were available (21/24, 87.5%) were of genotype D5. The results  indicate that multiplex real-time PCR might be a useful screening method for detecting and differentiating  rash-associated viruses in clinical specimens.
authors.affiliation: Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Osaka, Japan. a-kaida＠city.osaka.lg.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kaida
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kubo
authors.forename: Jun-Ichiro
authors.initials: J
authors.lastname: Sekiguchi
authors.forename: Minori
authors.initials: M
authors.lastname: Ohyama
authors.forename: Kaoru
authors.initials: K
authors.lastname: Goto
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hase
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Iritani
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005076:Exanthema / Q000453:epidemiology / Q000821:virology*; D005260:Female; D006801:Humans; D007223:Infant; D007564:Japan / Q000453:epidemiology; D008297:Male; D008459:Measles virus / Q000235:genetics / Q000302:isolation & purification*; D016732:Parvovirus B19, Human / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D019211:Roseolovirus / Q000235:genetics / Q000302:isolation & purification*; D012412:Rubella virus / Q000235:genetics / Q000302:isolation & purification*