title: Methods of sperm vitality assessment.
abstract: Sperm vitality is a reflection of the proportion of live, membrane-intact spermatozoa determined by either dye exclusion or osmoregulatory capacity under hypo-osmotic conditions. In this chapter we address the two most common methods of sperm vitality assessment: eosin-nigrosin staining and the hypo-osmotic swelling test, both utilized in clinical Andrology laboratories.
authors.affiliation: CReATe Fertility Center, Toronto, ON, Canada. sergey@createivf.com
authors.forename: Sergey I
authors.initials: SI
authors.lastname: Moskovtsev
authors.forename: Clifford L
authors.initials: CL
authors.lastname: Librach
mesh_terms: D002470:Cell Survival; D006801:Humans; D008297:Male; D009995:Osmosis; D055101:Semen Analysis / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods