title: Piperine, an LXRα antagonist, protects against hepatic steatosis and improves insulin signaling in mice fed a high-fat diet.
abstract: This study investigated the role of piperine in the transcriptional regulation of liver X receptor α (LXRα) and the effects of dietary piperine on high-fat diet (HFD)-induced hepatic steatosis and insulin resistance in mice. Furthermore, we explored the potential molecular mechanisms through which the protective effects of piperine may work. In the present study, piperine significantly reduced ligand-induced LXRα activity in a dose-dependent manner and gradually disrupted the interaction between ligand-bound LXRα and GST-CBP. In mice, an HFD supplemented with 0.05% piperine (PSD) significantly decreased body and liver weight as well as plasma and hepatic lipid levels. In agreement with our in vitro study, in mice fed an HFD, dietary piperine markedly decreased LXRα mRNA expression and its lipogenic target genes (i.e., SREBP1c, ChREBPα, FAS, and CD36). Piperine also significantly decreased plasma insulin and glucose concentrations, while increasing insulin sensitivity in mice fed an HFD. In addition, piperine downregulated the expression of genes involved in ER stress, including GRP78, activating transcription factor 6, and eukaryotic translation initiation factor 2α, and upregulated GLUT2 translocation from the cytosol to the plasma membrane in the livers of PSD mice. Piperine antagonized LXRα transcriptional activity by abolishing the interaction of ligand-bound LXRα with the co-activator CBP. The effects of piperine on hepatic lipid accumulation were likely regulated via alterations in LXRα-mediated lipogenesis in mice fed an HFD. Dietary piperine also led to reduced ER stress and increased insulin sensitivity and prevented hepatic insulin resistance in mice fed the HFD.
authors.affiliation: Department of Food and Nutrition, Yonsei University, Seoul, Republic of Korea.
authors.forename: Hyejeong
authors.initials: H
authors.lastname: Jwa
authors.forename: Youngshim
authors.initials: Y
authors.lastname: Choi
authors.forename: Ui-Hyun
authors.initials: UH
authors.lastname: Park
authors.forename: Soo-Jong
authors.initials: SJ
authors.lastname: Um
authors.forename: Seung Kew
authors.initials: SK
authors.lastname: Yoon
authors.forename: Taesun
authors.initials: T
authors.lastname: Park
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D052117:Benzodioxoles / Q000008:administration & dosage / Q000494:pharmacology*; D015153:Blotting, Western; D017931:DNA Primers; D004041:Dietary Fats / Q000008:administration & dosage*; D000091342:Endoplasmic Reticulum Chaperone BiP; D005234:Fatty Liver / Q000517:prevention & control*; D057809:HEK293 Cells; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D051379:Mice; D010880:Piperidines / Q000008:administration & dosage / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D053284:Polyunsaturated Alkamides / Q000008:administration & dosage / Q000494:pharmacology*; D015398:Signal Transduction
chemical_list: D000470:Alkaloids; D052117:Benzodioxoles; D017931:DNA Primers; D004041:Dietary Fats; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; C000717248:Hspa5 protein, mouse; D007328:Insulin; D010880:Piperidines; D053284:Polyunsaturated Alkamides; C008922:piperine