title: Identification by Q-PCR of Trypanosoma cruzi lineage and determination of blood meal sources in triatomine gut samples in México.
abstract: Triatomine vectors were collected on human dwellings in Michoacán México. Blood meal sources were identified by real time polymerase chain reaction (Q-PCR) using DNA extracted from triatomine guts. The assay was performed with one only specific primer set to amplify a fragment of the mitochondrial 12S ribosomal gene from vertebrate species. Also Trypanosoma cruzi parasites were detected in triatomine gut samples by microscopy and the positive infection was tested in mice. In addition T. cruzi discrete taxonomic units (DTUs) were identified by Q-PCR with two sets of primers that amplify the mini-circle region (miniexon) and 18S ribosomal mitochondrial gene. The sequences obtained from 18S ribosomal gene amplifications confirmed the presence of T. cruzi I and II lineages, and provide evidence of the presence of lineage TcIII and TcIV.
authors.affiliation: Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. Carpio y Plan de Ayala s/n, Col. Santo Tomás, CP 11340, México, DF, México.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Ibáñez-Cervantes
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martínez-Ibarra
authors.forename: Benjamín
authors.initials: B
authors.lastname: Nogueda-Torres
authors.forename: Eduardo
authors.initials: E
authors.lastname: López-Orduña
authors.forename: Ana L
authors.initials: AL
authors.lastname: Alonso
authors.forename: Cynthia
authors.initials: C
authors.lastname: Perea
authors.forename: Teresa
authors.initials: T
authors.lastname: Maldonado
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Hernández
authors.forename: Gloria
authors.initials: G
authors.lastname: León-Avila
mesh_terms: D000818:Animals; D001483:Base Sequence; D014355:Chagas Disease / Q000469:parasitology / Q000635:transmission; D005766:Gastrointestinal Contents / Q000737:chemistry*; D005838:Genotype; D006801:Humans; D007303:Insect Vectors / Q000469:parasitology*; D008800:Mexico; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D016415:Sequence Alignment; D013045:Species Specificity; D014224:Triatoma / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000235:genetics*; D014714:Vertebrates / Q000235:genetics
chemical_list: D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; C075526:RNA, ribosomal, 12S