title: A genome-wide screen to identify transcription factors expressed in pelvic Ganglia of the lower urinary tract.
abstract: Relative positions of neurons within mature murine pelvic ganglia based on expression of neurotransmitters have been described. However the spatial organization of developing innervation in the murine urogenital tract (UGT) and the gene networks that regulate specification and maturation of neurons within the pelvic ganglia of the lower urinary tract (LUT) are unknown. We used whole-mount immunohistochemistry and histochemical stains to localize neural elements in 15.5 days post coitus (dpc) fetal mice. To identify potential regulatory factors expressed in pelvic ganglia, we surveyed expression patterns for known or probable transcription factors (TF) annotated in the mouse genome by screening a whole-mount in situ hybridization library of fetal UGTs. Of the 155 genes detected in pelvic ganglia, 88 encode TFs based on the presence of predicted DNA-binding domains. Neural crest (NC)-derived progenitors within the LUT were labeled by Sox10, a well-known regulator of NC development. Genes identified were categorized based on patterns of restricted expression in pelvic ganglia, pelvic ganglia and urethral epithelium, or pelvic ganglia and urethral mesenchyme. Gene expression patterns and the distribution of Sox10+, Phox2b+, Hu+, and PGP9.5+ cells within developing ganglia suggest previously unrecognized regional segregation of Sox10+ progenitors and differentiating neurons in early development of pelvic ganglia. Reverse transcription-PCR of pelvic ganglia RNA from fetal and post-natal stages demonstrated that multiple TFs maintain post-natal expression, although Pax3 is extinguished before weaning. Our analysis identifies multiple potential regulatory genes including TFs that may participate in segregation of discrete lineages within pelvic ganglia. The genes identified here are attractive candidate disease genes that may now be further investigated for their roles in malformation syndromes or in LUT dysfunction.
authors.affiliation: Division of Genetic Medicine, Department of Medicine, Vanderbilt University School of Medicine Nashville, TN, USA.
authors.forename: Carrie B
authors.initials: CB
authors.lastname: Wiese
authors.forename: Sara
authors.initials: S
authors.lastname: Ireland
authors.forename: Nicole L
authors.initials: NL
authors.lastname: Fleming
authors.forename: Jing
authors.initials: J
authors.lastname: Yu
authors.forename: M Todd
authors.initials: MT
authors.lastname: Valerius
authors.forename: Kylie
authors.initials: K
authors.lastname: Georgas
authors.forename: Han Sheng
authors.initials: HS
authors.lastname: Chiu
authors.forename: Jane
authors.initials: J
authors.lastname: Brennan
authors.forename: Jane
authors.initials: J
authors.lastname: Armstrong
authors.forename: Melissa H
authors.initials: MH
authors.lastname: Little
authors.forename: Andrew P
authors.initials: AP
authors.lastname: McMahon
authors.forename: E Michelle
authors.initials: EM
authors.lastname: Southard-Smith