title: Intratumoral T but not B lymphocytes are related to clinical outcome in prostate cancer.
abstract: The number of tumor-infiltrating lymphocytes is functionally important and correlates with clinical outcome in several tumor entities. Herein we explore the impact of the density of T and B lymphocytes in prostate cancer tissue on prostate-specific antigen (PSA) recurrence after prostatectomy in 3261 prostate cancer tissue samples. The number of prostate cancer-infiltrating CD3-positive T cells and CD20-positive B cells per tissue spot in a tissue microarray format was determined by immunohistochemistry and was correlated with clinical and pathological data from the same patient cohort. Patients with very low and very high numbers of CD3-positive T cells per tissue spot had a significantly shorter PSA recurrence-free survival compared to patients with intermediate numbers of T cells (p = 0.0188). Furthermore, a high number of CD3-positive T cells per tissue spot was associated with fusion type prostate cancer identified by ERG expression analysis. The number of CD20-positive B cells per tissue spot was not associated with other clinical and histopathological parameters. This study indicates that the density of T but not B cells plays a functional role in the biology of prostate cancer and may have an impact on clinical outcome in this frequent neoplasia.
authors.affiliation: Departments of Oncology and Hematology, University Medical Center Hamburg-Eppendorf, Hubertus Wald Tumorzentrum (University Cancer Center Hamburg), Hamburg, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Flammiger
authors.forename: Florian
authors.initials: F
authors.lastname: Bayer
authors.forename: Ana
authors.initials: A
authors.lastname: Cirugeda-Kühnert
authors.forename: Hartwig
authors.initials: H
authors.lastname: Huland
authors.forename: Pierre
authors.initials: P
authors.lastname: Tennstedt
authors.forename: Ronald
authors.initials: R
authors.lastname: Simon
authors.forename: Sarah
authors.initials: S
authors.lastname: Minner
authors.forename: Carsten
authors.initials: C
authors.lastname: Bokemeyer
authors.forename: Guido
authors.initials: G
authors.lastname: Sauter
authors.forename: Thorsten
authors.initials: T
authors.lastname: Schlomm
authors.forename: Martin
authors.initials: M
authors.lastname: Trepel
mesh_terms: D000368:Aged; D018951:Antigens, CD20 / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D017252:CD3 Complex / Q000378:metabolism; D002470:Cell Survival; D006801:Humans; D007150:Immunohistochemistry; D018655:Lymphocyte Count; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D016016:Proportional Hazards Models; D011467:Prostate / Q000473:pathology / Q000601:surgery; D017430:Prostate-Specific Antigen / Q000097:blood; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000276:immunology* / Q000473:pathology / Q000601:surgery; D012044:Regression Analysis; D013601:T-Lymphocytes / Q000276:immunology*; D016896:Treatment Outcome
chemical_list: D018951:Antigens, CD20; D017252:CD3 Complex; D017430:Prostate-Specific Antigen