title: Inherently electroactive graphene oxide nanoplatelets as labels for single nucleotide polymorphism detection.
abstract: Graphene materials are being widely used in electrochemistry due to their versatility and excellent properties as platforms for biosensing. However, no records show the use of inherent redox properties of graphene oxide as a label for detection. Here for the first time we used graphene oxide nanoplatelets (GONPs) as electroactive labels for DNA analysis. The working signal comes from the reduction of the oxygen-containing groups present on the surface of GONPs. The different ability of the graphene oxide nanoplatelets to conjugate to DNA hybrids obtained with complementary, noncomplementary, and one-mismatch sequences allows the discrimination of single-nucleotide polymorphism correlated with Alzheimer's disease. We believe that our findings are very important to open a new route in the use of graphene oxide in electrochemistry.
authors.affiliation: Division of Chemistry & Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bonanni
authors.forename: Chun Kiang
authors.initials: CK
authors.lastname: Chua
authors.forename: Guanjia
authors.initials: G
authors.lastname: Zhao
authors.forename: Zdeněk
authors.initials: Z
authors.lastname: Sofer
authors.forename: Martin
authors.initials: M
authors.lastname: Pumera
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004252:DNA Mutational Analysis / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000648:ultrastructure; D010087:Oxides / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D010087:Oxides; D006108:Graphite