title: Expression of the Chlamydomonas reinhardtii sedoheptulose-1,7-bisphosphatase in Dunaliella bardawil leads to enhanced photosynthesis and increased glycerol production.
abstract: Bioengineering of photoautotrophic microalgae into CO(2) scrubbers and producers of value-added metabolites is an appealing approach in low-carbon economy. A strategy for microalgal bioengineering is to enhance the photosynthetic carbon assimilation through genetically modifying the photosynthetic pathways. The halotolerant microalgae Dunaliella possess a unique osmoregulatory mechanism, which accumulates intracellular glycerol in response to extracellular hyperosmotic stresses. In our study, the Calvin cycle enzyme sedoheptulose 1,7-bisphosphatase from Chlamydomonas reinhardtii (CrSBPase) was transformed into Dunaliella bardawil, and the transformant CrSBP showed improved photosynthetic performance along with increased total organic carbon content and the osmoticum glycerol production. The results demonstrate that the potential of photosynthetic microalgae as CO(2) removers could be enhanced through modifying the photosynthetic carbon reduction cycle, with glycerol as the carbon sink.
authors.affiliation: Department of Microbiology, National University of Singapore, Singapore, Singapore.
authors.forename: Lei
authors.initials: L
authors.lastname: Fang
authors.forename: Hui Xin
authors.initials: HX
authors.lastname: Lin
authors.forename: Chin Seng
authors.initials: CS
authors.lastname: Low
authors.forename: Mei Hui
authors.initials: MH
authors.lastname: Wu
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Chow
authors.forename: Yuan Kun
authors.initials: YK
authors.lastname: Lee
mesh_terms: D002244:Carbon / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000201:enzymology*; D005818:Genetic Engineering; D005990:Glycerol / Q000378:metabolism*; D058086:Microalgae / Q000201:enzymology* / Q000254:growth & development; D030781:Organisms, Genetically Modified / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010788:Photosynthesis; D055049:Salt Tolerance; D013213:Starch / Q000378:metabolism
chemical_list: D002244:Carbon; D013213:Starch; D010744:Phosphoric Monoester Hydrolases; C022627:sedoheptulose-bisphosphatase; D005990:Glycerol