title: [Nuclear matrix proteins differentially expressed in human prostate cancer cell lines and benign prostatic hyperplasia epithelial cell line].
abstract: OBJECTIVE
  To compare the expression of nuclear matrix proteins (NMPs) in benign prostatic hyperplasia (BPH) epithelial cell line BPH-1 versus those in androgen-dependent human prostate cancer cell line LNCap and androgen-independent prostate cancer cell line PC-3.
  
  
  METHODS
  We isolated NMPs from the BPH-1, LNCap and PC-3 cell lines by 2-dimensional electrophoresis (2-DE), analyzed the differentially expressed proteins by matrix-assisted laser desorption / ionization time of flight mass spectrometry (MALDI-TOF-MS), and identified them by peptide mass fingerprint and database searching.
  
  
  RESULTS
  We successfully obtained well-resolved reproducible 2-DE patterns of NMPs in human prostate cancer cell lines, identified 12 differentially expressed NMPs including enzymes, regulatory proteins, RNA-binding protein and various other factors, 3 up-regulated and 9 down-regulated in prostate cancer cell lines.
  
  
  CONCLUSIONS
  There are obvious differences in the expressions of NMPs between human prostate cancer cell lines and benign prostatic hyperplasia epithelial cell line.
authors.affiliation: Graduate School, Peking Union Medical College, Beijing 100730, China.
authors.forename: Tie-Cheng
authors.initials: TC
authors.lastname: Sun
authors.forename: Ling
authors.initials: L
authors.lastname: Xin
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Song
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Ning
authors.forename: Li-Yuan
authors.initials: LY
authors.lastname: Han
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: He-Ming
authors.initials: HM
authors.lastname: Yu
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Wang
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D034521:Nuclear Matrix-Associated Proteins; D020543:Proteome