title: Bacterial degradation and reduction in the estrogen activity of 4-nonylphenol.
abstract: Bacteria capable of degrading 4-nonylphenol  (NP)  were isolated and identified, and their ability to degrade NP was determined. The screening of microorganisms in river water and soil led to a collection of 23 strains of bacteria and five strains of fungi. Two strains of bacteria, identified as Pseudomonas sp. and Acidovorax sp., possessed great ability for degrading NP. The NP degradation rate of Pseudomonas sp. did not change with the NP concentration  (50-100mg/L) . In contrast, the NP degradation rate of Acidovorax sp. increased with increasing NP concentration. Acidovorax sp. possessed the greatest NP degradation activity at 35°C. No NP degradation activity was observed for Pseudomonas sp. at temperatures higher than 30°C. Even when non-NP carbon sources such as glucose or sucrose were added, the NP degradation rates for both bacteria did not decrease. In addition, the estrogenic activity of NP decreased depending on the amount of NP residues determined by the yeast two-hybrid system.
authors.affiliation: Faculty of Applied Bioscience, Kanagawa Institute of Technology, 1030 Shimo-Ogino, Atsugi, Kanagawa 243-0292, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Watanabe
authors.forename: Yumie
authors.initials: Y
authors.lastname: Hori
authors.forename: Shintaro
authors.initials: S
authors.lastname: Nishimura
authors.forename: Akane
authors.initials: A
authors.lastname: Takagi
authors.forename: Mikio
authors.initials: M
authors.lastname: Kikuchi
authors.forename: Jun
authors.initials: J
authors.lastname: Sawai
mesh_terms: D001673:Biodegradation, Environmental; D042621:Comamonadaceae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D045483:Rivers / Q000382:microbiology*; D012988:Soil Microbiology
chemical_list: D004967:Estrogens; D010636:Phenols; C025256:nonylphenol