title: Methods for sperm-mediated gene transfer.
abstract: The transgenic technologies represent potent biotechnological tools that allow the generation of genetically modified animals useful for basic research and for biomedical, veterinary, and agricultural applications. Among transgenic techniques, we describe here the sperm-mediated gene transfer methods that is gene transfer based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to oocyte during fertilization, producing genetically modified animals with high efficiency.
authors.affiliation: Department of Surgical Sciences, University of Milano-Bicocca, Monza, Italy. marialuisa.lavitrano@unimib.it
authors.forename: Marialuisa
authors.initials: M
authors.lastname: Lavitrano
authors.forename: Roberto
authors.initials: R
authors.lastname: Giovannoni
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Cerrito
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004624:Embryo Transfer / Q000379:methods; D005307:Fertilization in Vitro / Q000379:methods; D018014:Gene Transfer Techniques; D008297:Male; D051379:Mice; D053625:Sperm Retrieval; D013094:Spermatozoa / Q000378:metabolism*; D013552:Swine