title: Adiposoft: automated software for the analysis of white adipose tissue cellularity in histological sections.
abstract: The accurate estimation of the number and size of cells provides relevant information on the kinetics of growth and the physiological status of a given tissue or organ. Here, we present Adiposoft, a fully automated open-source software for the analysis of white adipose tissue cellularity in histological sections. First, we describe the sequence of image analysis routines implemented by the program. Then, we evaluate our software by comparing it with other adipose tissue quantification methods, namely, with the manual analysis of cells in histological sections (used as gold standard) and with the automated analysis of cells in suspension, the most commonly used method. Our results show significant concordance between Adiposoft and the other two methods. We also demonstrate the ability of the proposed method to distinguish the cellular composition of three different rat fat depots. Moreover, we found high correlation and low disagreement between Adiposoft and the manual delineation of cells. We conclude that Adiposoft provides accurate results while considerably reducing the amount of time and effort required for the analysis.
authors.affiliation: Imaging Unit and Cancer Imaging Laboratory, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Galarraga
authors.forename: Javier
authors.initials: J
authors.lastname: Campión
authors.forename: Arrate
authors.initials: A
authors.lastname: Muñoz-Barrutia
authors.forename: Noemí
authors.initials: N
authors.lastname: Boqué
authors.forename: Haritz
authors.initials: H
authors.lastname: Moreno
authors.forename: José Alfredo
authors.initials: JA
authors.lastname: Martínez
authors.forename: Fermín
authors.initials: F
authors.lastname: Milagro
authors.forename: Carlos
authors.initials: C
authors.lastname: Ortiz-de-Solórzano
mesh_terms: D052436:Adipose Tissue, White / Q000166:cytology*; D000818:Animals; D001331:Automation; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012984:Software