title: Influence of apolipoprotein A-V on hepatocyte lipid droplet formation.
abstract: Apolipoprotein A-V (apoA-V) is postulated to modulate intra-hepatic triglyceride (TG) trafficking. Stably transfected McA-RH7777 hepatocarcinoma cells expressing human apoA-V displayed enhanced neutral lipid staining while conditioned media from these cells had 40±8% less TG than cells transfected with a control vector. To obtain homogeneous cell lines expressing different amounts of apoA-V, a strategy of clonal selection was pursued. Immunoblot analysis of two distinct apoA-V stable cell lines yielded one that expresses low amounts of apoA-V and another that expresses higher amounts. Confocal fluorescence microscopy of control cells and cells expressing low levels of apoA-V had similar numbers of lipid droplets while cells expressing higher amounts of apoA-V had twice as many lipid droplets, on average. Thus, apoA-V expression promotes lipid droplet accumulation in these cells.
authors.affiliation: Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609, USA.
authors.forename: Xuan
authors.initials: X
authors.lastname: Gao
authors.forename: Trudy M
authors.initials: TM
authors.lastname: Forte
authors.forename: Robert O
authors.initials: RO
authors.lastname: Ryan
mesh_terms: D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D003600:Cytosol / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D014162:Transfection; D014280:Triglycerides / Q000378:metabolism*
chemical_list: C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A; D014280:Triglycerides