title: Survivin, MMP-2, MT1-MMP, and TIMP-2: their impact on survival, implantation, and proliferation of endometriotic tissues.
abstract: In order to study survivin, matrix metalloproteinases (MMP-2), membranous type 1 matrix metalloproteinase (MT1-MMP), and tissue inhibitor metalloproteinase-2 (TIMP-2) expression immunohistochemically in endometriotic tissues and normal endometrium, our retrospective study considered 194 patients affected by endometriosis and 71 patients with normal endometrium. Tissue microarrays were created from paraffin-embedded blocks; immunohistochemistry was used to assess protein expression. In endometriotic tissues, survivin was expressed at a higher level than in normal endometrium; its glandular expression level was higher in non-ovarian than in ovarian endometriotic tissues and lower in stromal components. Endometrial tissues from women without endometriosis and endometriotic tissues had different matrix metalloproteinase expression profiles. MMP-2 and MT1-MMP correlated with TIMP-2 in endometriotic tissues. Furthermore, in endometriotic tissues, expression of survivin, aurora B kinase, and Ki-67 showed a significant positive correlation, which indicates a role in cellular proliferation that could be closely linked to its anti-apoptotic activity in endometriosis development. Our results imply a role for matrix metalloproteinases in endometriosis invasiveness; correlation of their expression with that of TIMP-2 underscores its possible key regulatory role.
authors.affiliation: Clinic of Obstetrics and Gynecology, University of Udine, Piazzale SM della Misericordia, 15-33100 Udine, Italy.
authors.forename: Ambrogio P
authors.initials: AP
authors.lastname: Londero
authors.forename: Angelo
authors.initials: A
authors.lastname: Calcagno
authors.forename: Tiziana
authors.initials: T
authors.lastname: Grassi
authors.forename: Stefania
authors.initials: S
authors.lastname: Marzinotto
authors.forename: Maria
authors.initials: M
authors.lastname: Orsaria
authors.forename: Carlo Alberto
authors.initials: CA
authors.lastname: Beltrami
authors.forename: Diego
authors.initials: D
authors.lastname: Marchesoni
authors.forename: Laura
authors.initials: L
authors.lastname: Mariuzzi
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D010049:Ovarian Diseases / Q000378:metabolism / Q000473:pathology*; D012189:Retrospective Studies; D000077022:Survivin; D046888:Tissue Array Analysis; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism*
chemical_list: C107489:BIRC5 protein, human; D015415:Biomarkers; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; C560823:TIMP2 protein, human; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2; D053511:Matrix Metalloproteinase 14