title: [In vivo study of antifertility and anti-inflammatory effects of compound Kucen gel].
abstract: OBJECTIVE
  To study the antifertility and anti-inflammatory effects of compound Kucen gel in vivo.
  
  
  METHODS
  As antifertility experiment, we randomly divided 60 female SD rats into six groups of equal number: normal saline, blank gel, low-, medium- and high-dose compound Kucen gel (0.05, 0.10 and 0.15 g/g), and positive control (4% nonoxynol gel) to receive intravaginal administration of 200 microl of respective agent, followed by copulation with male rats in a 1:1 ratio. At 12 days after successful mating, the female rats were dissected for calculation of the embryos and the rate of contraception. As an anti-inflammatory trial, we established a mouse model of inflammation by applying xylene to the pinna, and equally randomized 60 Kunming mice to six groups as in the former experiment. We determined the degrees and average rates of swelling inhibition in the left ear.
  
  
  RESULTS
  High-dose compound Kucen gel achieved a fertility-inhibition rate of 100% in the female rats, the number of embryos significantly lower than in the normal saline group (0.00 +/- 0.00 vs 11.00 +/- 2. 00, P < 0.05), but with no statistically insignificant difference from that of the positive control (0.00 +/- 0.00, P > 0.05). High-dose compound Kucen gel also markedly suppressed swelling in the left ear of the mice, with an inhibition rate of 52.3%, the average swelling degree significantly lower than in the normal saline group (10.17 +/- 2.56 vs 21.32 +/- 3.17, P < 0.01), but not remarkably different from that of the positive control (8.53 +/- 1.89, P > 0.05).
  
  
  CONCLUSIONS
  Compound Kucen gel, with its strong antifertility and anti-inflammatory effects, deserves further study and clinical application.
authors.affiliation: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Jan-Ping
authors.initials: JP
authors.lastname: Miu
authors.forename: Can-Feng
authors.initials: CF
authors.lastname: Lu
authors.forename: Xiang-Hu
authors.initials: XH
authors.lastname: Meng
authors.forename: Jing
authors.initials: J
authors.lastname: Ding
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Zang
authors.forename: Qian
authors.initials: Q
authors.lastname: Su
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003270:Contraceptive Agents / Q000494:pharmacology*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005782:Gels; D007249:Inflammation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D003270:Contraceptive Agents; D004365:Drugs, Chinese Herbal; D005782:Gels