title: From quantitative protein complex analysis to disease mechanism.
abstract: Interest in the field of cilia biology and cilia-associated diseases - ciliopathies - has strongly increased over the last few years. Proteomic technologies, especially protein complex analysis by affinity purification-based methods, have been used to decipher various basic but also disease-associated mechanisms. This review focusses on some selected recent studies using affinity purification-based protein complex analysis, thereby exemplifying the great possibilities this technology offers.
authors.affiliation: Division of Experimental Ophthalmology and Medical Proteome Center, Centre for Ophthalmology, Eberhard Karls University Tübingen, Nägelestr. 5, D-72074 Tübingen, Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Texier
authors.forename: N
authors.initials: N
authors.lastname: Kinkl
authors.forename: K
authors.initials: K
authors.lastname: Boldt
authors.forename: M
authors.initials: M
authors.lastname: Ueffing
mesh_terms: D000818:Animals; D002923:Cilia / Q000737:chemistry / Q000235:genetics; D002925:Ciliary Motility Disorders / Q000378:metabolism*; D005407:Flagella / Q000737:chemistry; D006801:Humans; D057130:Leber Congenital Amaurosis / Q000209:etiology; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis* / Q000502:physiology; D011506:Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D008565:Membrane Proteins; D011506:Proteins