title: A real-time PCR detection system for the bois noir and flavescence dorée phytoplasmas and quantification of the target DNA.
abstract: The real-time PCR detection system for grapevine yellows phytoplasmas described here is composed of two assays for group-specific detection of flavescence dorée (FD) and bois noir (BN) phytoplasmas and a universal phytoplasma assay. It uses hydrolysis minor groove binder probes (TaqMan-MGB). The addition of an assay for amplification of plant DNA co-extracted with phytoplasma DNA provides a further quality control for the DNA extraction and PCR amplification for each sample. The detection system described is reliable, specific, sensitive, and easily applicable to fast, high-throughput diagnosis of grapevine yellows phytoplasmas. In addition to the detection system, an approach for the quantification of phytoplasmas in the sample is described.
authors.affiliation: ational Institute of Biology, Ljubljana, Slovenia. natasa.mehle@nib.si
authors.forename: Nataša
authors.initials: N
authors.lastname: Mehle
authors.forename: Nina
authors.initials: N
authors.lastname: Prezelj
authors.forename: Matjaž
authors.initials: M
authors.lastname: Hren
authors.forename: Jana
authors.initials: J
authors.lastname: Boben
authors.forename: Kristina
authors.initials: K
authors.lastname: Gruden
authors.forename: Maja
authors.initials: M
authors.lastname: Ravnikar
authors.forename: Marina
authors.initials: M
authors.lastname: Dermastia
mesh_terms: D019295:Computational Biology / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics; D045582:Phytoplasma / Q000235:genetics / Q000302:isolation & purification*; D010935:Plant Diseases / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*
chemical_list: D004269:DNA, Bacterial