title: Angiogenic effect of endothelial progenitor cells transfected with telomerase reverse transcriptase on peritubular microvessel in five out of six subtotal nephrectomy rats.
abstract: Renal disease is caused by tubular interstitial injury and renal interstitial fibrosis. Previous studies have shown that transplantation of endothelial progenitor cells (EPCs) may provide an appropriate treatment for repair and reversing renal pathology. However, EPCs are typically low in abundance and have poor replication ability. Therefore, the this study investigated the use of EPCs transfected with the telomerase reverse transcriptase (TERT) in rats that had undergone five out of six subtotal nephrectomy. This study determined the effects of EPC transplantation on renal function, renal interstitial fibrosis, and peritubular capillary angiogenesis. Five groups of rats were investigated: sham group, model group (five out of six subtotal nephrectomy), EPCs-N group (transplantation with EPCs), pZ-TERT-EPCs-N group (transplantation with EPCs transfected with TERT), and pZ-EPCs-N group (transplantation with EPCs transfected with empty plasmid). At weeks 4, 8, and 12 after transplantation, renal function, renal interstitial fibrosis, and peritubular microvessel density (MVD) were investigated. EPCs transfected with TERT gene showed decreased in vitro senescence, apoptosis, and proliferative ability was significantly enhanced (p < 0.05). Furthermore, rat transplanted with EPCs transfected with TERT showed significantly reduced renal interstitial fibrosis and increased endogenous creatinine clearance rate and peritubular MVD (p < 0.05). The transplantation of EPCs expressing TERT into five out of six subtotal nephrectomy rats was shown to improve renal function, reduce loss of peritubular microvessel, and inhibit progression of renal interstitial fibrosis. These studies provide the basis for a potential treatment of renal disease using genetically modified EPCs.
authors.affiliation: Laboratory of Pediatric Nephrology, The Second Xiangya Hospital, Central South University & Hunan Province Clinical Center of Pediatric Nephrology, Changsha, PR China.
authors.forename: Lanjun
authors.initials: L
authors.lastname: Shuai
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.forename: Qingnan
authors.initials: Q
authors.lastname: He
authors.forename: Xiqiang
authors.initials: X
authors.lastname: Dang
authors.forename: Haixia
authors.initials: H
authors.lastname: Chen
authors.forename: Pin
authors.initials: P
authors.lastname: Zhou
authors.forename: Zhuwen
authors.initials: Z
authors.lastname: Yi
authors.forename: Xiaojie
authors.initials: X
authors.lastname: He
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology*; D005260:Female; D007684:Kidney Tubules / Q000098:blood supply*; D055806:Microvessels; D018919:Neovascularization, Physiologic; D009392:Nephrectomy / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000502:physiology*; D019098:Telomerase; D014162:Transfection / Q000379:methods*
chemical_list: D019098:Telomerase