title: BD PuraMatrix peptide hydrogel as a culture system for human fetal Schwann cells in spinal cord regeneration.
abstract: BD PuraMatrix peptide hydrogel, a three-dimensional cell culture model of nanofiber scaffold derived from the self-assembling peptide RADA16, has been applied to regenerative tissue repair in order to develop novel nanomedicine systems. In this study with PuraMatrix, self-assembling nanofiber scaffold (SAPNS) and Schwann cells (SCs) were isolated from human fetal sciatic nerves, cultured within SAPNS, and then transplanted into the spinal cord after injury (SCI) in rats. First, the peptide nanofiber scaffold was evaluated via scanning electron microscopy and atomic force microscopy. With phase-contrast microscopy, the appearance of representative human fetal SCs encapsulated in PuraMatrix on days 3, 5, and 7 in 12-well plates was revealed. The Schwann cells in PuraMatrix were cultured for 2 days, and the SCs had active proliferative potential. Spinal cord injury was induced by placing a 35-g weight on the dura of T9-T10 segments for 15 min, followed by in vivo treatment with SAPNS and human fetal SCs (100,000 cells/10 μl/injection) grafted into spinal cord 7 days after SCI. After treatment, the recovery of motor function was assessed periodically using the Basso, Beattie, and Bresnahan scoring system. Eight weeks after grafting, animals were perfusion fixed, and the survival of implanted cells was analyzed with antibody recognizing SCs. Immunohistochemical analysis of grafted lumber segments at 8 weeks after grafting revealed reduced asterogliosis and considerably increased infiltration of endogenous S100(+) cells into the injury site, suggesting that PuraMatrix may play an important role in the repair observed after SAPNS and human fetal SC transplantation.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fateme
authors.initials: F
authors.lastname: Moradi
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Bahktiari
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Joghataei
authors.forename: Maliheh
authors.initials: M
authors.lastname: Nobakht
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Hasanzadeh
authors.forename: Ali
authors.initials: A
authors.lastname: Fallah
authors.forename: Sam
authors.initials: S
authors.lastname: Zarbakhsh
authors.forename: Leila Beigom
authors.initials: LB
authors.lastname: Hejazian
authors.forename: Maryam
authors.initials: M
authors.lastname: Shirmohammadi
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Maleki
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured / Q000166:cytology / Q000637:transplantation; D005911:Gliosis / Q000209:etiology / Q000473:pathology; D006801:Humans; D020100:Hydrogels; D008124:Locomotion; D008297:Male; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D010455:Peptides; D061251:Primary Cell Culture / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D012038:Regeneration; D012583:Schwann Cells / Q000166:cytology / Q000637:transplantation*; D012584:Sciatic Nerve / Q000166:cytology* / Q000196:embryology; D013116:Spinal Cord / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000601:surgery*; D054457:Tissue Scaffolds; D014183:Transplantation, Heterologous
chemical_list: D020100:Hydrogels; D010455:Peptides; C546681:RADA16-I