title: The Nup153-Nup50 protein interface and its role in nuclear import.
abstract: Interactions between Nup50 and soluble transport factors underlie the efficiency of certain nucleocytoplasmic transport pathways. The platform on which these interactions take place is important to building a complete understanding of nucleocytoplasmic trafficking. Nup153 is the nucleoporin that provides this scaffold for Nup50. Here, we have delineated requirements for the interaction between Nup153 and Nup50, revealing a dual interface. An interaction between Nup50 and a region in the unique N-terminal region of Nup153 is critical for the nuclear pore localization of Nup50. A second site of interaction is at the distal tail of Nup153 and is dependent on importin α. Both of these interactions involve the N-terminal domain of Nup50. The configuration of the Nup153-Nup50 partnership suggests that the Nup153 scaffold provides not just a means of pore targeting for Nup50 but also serves to provide a local environment that facilitates bringing Nup50 and importin α together, as well as other soluble factors involved in transport. Consistent with this, disruption of the Nup153-Nup50 interface decreases efficiency of nuclear import.
authors.affiliation: Department of Oncological Sciences, University of Utah, Salt Lake City, Utah 84112, USA.
authors.forename: Masaki
authors.initials: M
authors.lastname: Makise
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Mackay
authors.forename: Suzanne
authors.initials: S
authors.lastname: Elgort
authors.forename: Sunita S
authors.initials: SS
authors.lastname: Shankaran
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Adam
authors.forename: Katharine S
authors.initials: KS
authors.lastname: Ullman
mesh_terms: D021581:Active Transport, Cell Nucleus; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D022022:Nuclear Pore / Q000737:chemistry; D028861:Nuclear Pore Complex Proteins / Q000737:chemistry / Q000502:physiology*; D009687:Nuclear Proteins / Q000737:chemistry / Q000502:physiology*; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D028901:alpha Karyopherins / Q000737:chemistry
chemical_list: C079377:NUP153 protein, human; C121702:NUP50 protein, human; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D028901:alpha Karyopherins; D049452:Green Fluorescent Proteins; D005982:Glutathione Transferase