title: Development of a novel enzyme-linked immunosorbent assay (ELISA) for measurement of serum CTRP1: a pilot study: measurement of serum CTRP1 in healthy donors and patients with metabolic syndrome.
abstract: BACKGROUND
  Complement C1q tumor necrosis factor-related protein 1 (CTRP1), a recently identified adipokine, was found to stimulate aldosterone production. Obesity and metabolic syndrome are frequently associated with elevated levels of aldosterone. Therefore, it would be interesting to investigate whether the secretion of CTRP1 in human serum is associated with obesity as well as with hypertension.
  
  
  OBJECTIVE
  This study evaluated serum CTRP1 concentrations in healthy individuals and patients with metabolic syndrome.
  
  
  METHODS
  Serum samples from 61 healthy individuals and 46 patients with metabolic syndrome were measured for CTRP1 by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Correlation analyses showed that serum CTRP1 in healthy individuals did not correlate with BMI, leptin, TG, HDL-CH, and LDL-CH; however, in patients with metabolic syndrome, CTRP1 correlated with glucose, HbA1c and BMI. CTRP1 level was significantly higher in subjects with metabolic syndrome compared to healthy subjects.
  
  
  CONCLUSIONS
  Our results support the hypothesis that adipokine CTRP1 is associated with metabolic syndrome and obesity compared to healthy individuals.
authors.affiliation: Department of Animal Physiology and Immunology, Faculty of Science, Masaryk University, Brno, Czech Republic. 106314@is.muni.cz
authors.forename: L
authors.initials: L
authors.lastname: Chalupova
authors.forename: A
authors.initials: A
authors.lastname: Zakovska
authors.forename: K
authors.initials: K
authors.lastname: Adamcova
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D015992:Body Mass Index; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D020738:Leptin / Q000097:blood; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood*; D008875:Middle Aged; D009765:Obesity / Q000097:blood; D010865:Pilot Projects; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D020738:Leptin; D011506:Proteins; D014280:Triglycerides; C512356:C1QTNF1 protein, human; C517652:hemoglobin A1c protein, human