title: Effects of Cervi cornus Colla (deer antler glue) in the reconstruction of a skin equivalent model.
abstract: The aim of this study was to investigate the effects of Cervi cornus Colla (CCC) in the reconstruction of skin equivalent (SE). H&E staining showed that SE containing hyaluronic acid (HA) or HA and CCC had a thicker epidermis than the control SE. Immunohistochemical staining showed that p63 was mainly present at the basal layer of the epidermis in the HA and CCC model. Involucrin was obviously expressed in the upper layer of the epidermis in the HA and CCC model. Moreover, we observed that integrins α6 and β1 were strongly expressed along the basement membrane zone in the HA and CCC model, in which the dermis expressing type I collagen was more compact. In conclusion, our data indicate that CCC contributed to the formation of epidermis, basement membrane, and extracellular matrix in the reconstruction of SE and suggest that CCC may be a useful adjuvant in the reconstruction of SE.
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, 221 Heukseok-dong Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Jandi
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, 221 Heukseok-dong Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Hyo-Soon
authors.initials: HS
authors.lastname: Jeong
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, 221 Heukseok-dong Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Hailan
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, 221 Heukseok-dong Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Kwang Jin
authors.initials: KJ
authors.lastname: Baek
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, 221 Heukseok-dong Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Nyoun Soo
authors.initials: NS
authors.lastname: Kwon
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, 221 Heukseok-dong Dongjak-gu, Seoul, 156-756, Republic of Korea.
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Yun
authors.affiliation: Department of Dermatology, Seoul National University Bundang Hospital, 300 Gumi-dong, Bundang-gu, Seongnam-si, Kyoungki-do, 463-707, Republic of Korea.
authors.forename: Hye-Ryung
authors.initials: HR
authors.lastname: Choi
authors.affiliation: Department of Dermatology, Seoul National University Bundang Hospital, 300 Gumi-dong, Bundang-gu, Seongnam-si, Kyoungki-do, 463-707, Republic of Korea.
authors.forename: Kyoung-Chan
authors.initials: KC
authors.lastname: Park
authors.affiliation: Department of Biochemistry, Chung-Ang University College of Medicine, 221 Heukseok-dong Dongjak-gu, Seoul, 156-756, Republic of Korea. ds_kim@cau.ac.kr.
authors.forename: Dong-Seok
authors.initials: DS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000999:Antlers / Q000737:chemistry*; D001485:Basement Membrane / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D018920:Coculture Techniques; D024042:Collagen Type I / Q000378:metabolism; D003670:Deer; D020405:Dermis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology; D007150:Immunohistochemistry; D039503:Integrin alpha6 / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D014157:Transcription Factors / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D039503:Integrin alpha6; D019012:Integrin beta1; C115051:TP63 protein, human; D014020:Tissue Extracts; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; D006820:Hyaluronic Acid