title: Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil.
abstract: To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the non-structural viral protein 1 (NS1) gene of HBoV-1 and HBoV-3 were used. Twelve HBoV-positive samples were further characterised via genomic sequencing and phylogenetic analysis. Of the samples tested, 5.8% (n = 44) were positive for HBoV-1 or HBoV-3 and co-infection was observed in 14 (31.8%) of the 44 HBoV-positive samples. Nine of the 14 samples were also positive for Rotavirus A and five were positive for Aichi virus. The genomic sequencing of the NS1 partial sequence of 12 HBoV-samples showed that 11 samples were characterised as HBoV-1 and that one was characterised as HBoV-3. The phylogenetic analysis showed that the HBoV-1 samples had a high sequence homology to others previously identified in China, Sweden and Brazil. This is the first study conducted in the Central-West Region of Brazil to detect HBoV-1 and HBoV-3 in faecal samples from children with acute gastroenteritis. Further studies are required to define the role of HBoVs as aetiological agents of gastroenteritis.
authors.affiliation: Laboratório de Virologia Humana, Instituto de Patologia Tropical e Saúde Pública, Goiânia, GO, Brasil.
authors.forename: Teresinha Teixeira de
authors.initials: TT
authors.lastname: Sousa
authors.forename: Menira
authors.initials: M
authors.lastname: Souza
authors.forename: Fabíola Souza
authors.initials: FS
authors.lastname: Fiaccadori
authors.forename: Ana Maria Tavares
authors.initials: AM
authors.lastname: Borges
authors.forename: Paulo Sucasas da
authors.initials: PS
authors.lastname: Costa
authors.forename: Divina das Dôres de Paula
authors.initials: Dd
authors.lastname: Cardoso
mesh_terms: D000208:Acute Disease; D001938:Brazil / Q000453:epidemiology; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology / Q000821:virology*; D056661:Human bocavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012621:Seasons
chemical_list: D004279:DNA, Viral