title: miR-125b regulates the proliferation of glioblastoma stem cells by targeting E2F2.
abstract: microRNAs (miRNAs) play important role in regulating cancer stem cell self-renewal and differentiation, but the expression prolife of miRNAs in glioma stem cells (GSCs) has not been addressed. Here, we found that CD133 positive GSCs possess a unique miRNAs profile compared to CD133 negative glioblastoma cells. miR-125b, as one of neuronal miRNAs, is the most significantly down-regulated miRNAs and overexpression of miR-125b inhibits the proliferation of CD133 positive GSCs and reduces the expression of "stem" marker. Furthermore, two binding sites for miR-125b are identified in the 3'UTR of E2F2 and overexpression of miR-125b in CD133 positive GSCs represses the endogenous level of E2F2 protein. This study demonstrated that miR-125b plays important roles in regulating the proliferation of GSCs by directly targeting E2F2.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wu
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.forename: Xiangzhong
authors.initials: X
authors.lastname: Zhao
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Jianpeng
authors.initials: J
authors.lastname: Wang
authors.forename: Fengxia
authors.initials: F
authors.lastname: Wang
authors.forename: Shousong
authors.initials: S
authors.lastname: Cao
authors.forename: XiuKun
authors.initials: X
authors.lastname: Lin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000071916:AC133 Antigen; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D050703:E2F2 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism*; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D020413:3' Untranslated Regions; D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D050703:E2F2 Transcription Factor; C493761:E2F2 protein, human; D006023:Glycoproteins; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; C000606023:PROM1 protein, human; D010455:Peptides