title: Conjugation of transferrin to azide-modified CdSe/ZnS core-shell quantum dots using cyclooctyne click chemistry.
abstract: Twinkle twinkle quantum dot: Conjugation of biomolecules to azide-modified quantum dots (QDs) through a bifunctional linker, using strain-promoted azide-alkyne cycloaddition with the QD and a squaramide linkage to the biomolecule (see scheme). Transferrin-conjugated QDs were internalized by transferrin-receptor expressing HeLa cells.
authors.affiliation: School of Chemistry and Bio21 Institute, University of Melbourne, Parkville, Vic 3010, Australia. cschi@unimelb.edu.au
authors.forename: Christine
authors.initials: C
authors.lastname: Schieber
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bestetti
authors.forename: Jet Phey
authors.initials: JP
authors.lastname: Lim
authors.forename: Anneke D
authors.initials: AD
authors.lastname: Ryan
authors.forename: Tich-Lam
authors.initials: TL
authors.lastname: Nguyen
authors.forename: Robert
authors.initials: R
authors.lastname: Eldridge
authors.forename: Anthony R
authors.initials: AR
authors.lastname: White
authors.forename: Paul A
authors.initials: PA
authors.lastname: Gleeson
authors.forename: Paul S
authors.initials: PS
authors.lastname: Donnelly
authors.forename: Spencer J
authors.initials: SJ
authors.lastname: Williams
authors.forename: Paul
authors.initials: P
authors.lastname: Mulvaney
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D019187:Cadmium Compounds / Q000737:chemistry*; D057930:Click Chemistry / Q000379:methods; D034242:Cyclooctanes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D014168:Transferrin / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D000480:Alkynes; D001386:Azides; D019187:Cadmium Compounds; D034242:Cyclooctanes; D018036:Selenium Compounds; D013440:Sulfides; D014168:Transferrin; D017967:Zinc Compounds; C058667:cadmium selenide; C031238:zinc sulfide