title: Distribution of human papillomavirus genotype and cervical neoplasia among women with abnormal cytology in Beijing, China.
abstract: OBJECTIVE
  To understand the relationship between human papillomavirus (HPV) genotype distribution and cervical neoplasia among women with abnormal cytology in Beijing.
  
  
  METHODS
  In a study in Beijing, China, participants underwent a gynecologic examination between September 2006 and December 2009. Those with abnormal cytology underwent colposcopy, biopsy, and HPV testing. HPV was typed by flow-through hybridization on formalin-fixed paraffin-embedded tissue.
  
  
  RESULTS
  Among 6339 women examined, 533 with abnormal cytology underwent HPV testing. The prevalence of high-risk HPV (HR-HPV) subtypes was 29.8%. HPV-16 was the most common genotype (12.8%), followed by HPV-33, HPV-58, HPV-66, HPV-18, and HPV-52. The cytologic and histopathologic grade clearly increased with a higher prevalence of HR-HPV infection. Both HPV-16 and HPV-33 were significantly associated with grade CIN2+ lesions (P<0.05). By contrast, HPV-18 and HPV-66 were not associated with cervical lesion severity. HR-HPV infection was most common among women aged 30-34 years (35.0%).
  
  
  CONCLUSIONS
  HPV-16, HPV-58, HPV-33, HPV-66, HPV-18, and HPV-52 were the most common genotypes in Beijing. HPV-16 and HPV-33 might be associated with CIN2+ lesions. HPV-18 and HPV-66 were not associated with high-grade cervical lesions. The present results might help to improve HPV vaccination and cervical cancer prevention programs in Beijing.
authors.affiliation: Department of Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
authors.forename: Ren
authors.initials: R
authors.lastname: Hou
authors.forename: Caiyan
authors.initials: C
authors.lastname: Xu
authors.forename: Songwen
authors.initials: S
authors.lastname: Zhang
authors.forename: Minghui
authors.initials: M
authors.lastname: Wu
authors.forename: Weiyuan
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000328:Adult; D017677:Age Distribution; D002681:China / Q000453:epidemiology; D003127:Colposcopy; D005260:Female; D051456:Gene Flow; D005838:Genotype; D006801:Humans; D006824:Hybridization, Genetic; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence; D011446:Prospective Studies; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology*