title: Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum.
abstract: Protein tyrosine phosphatases (PTPs) play an essential role in the regulation of cell differentiation in pathogenic trypanosomatids. In this study, we describe a PTP expressed by the non-pathogenic protozoan Trypanosoma rangeli (TrPTP2). The gene for this PTP is orthologous to the T. brucei TbPTP1 and Trypanosoma cruzi (TcPTP2) genes. Cloning and expression of the TrPTP2 and TcPTP2 proteins allowed anti-PTP2 monoclonal antibodies to be generated in BALB/c mice. When expressed by T. rangeli epimastigotes and trypomastigotes, native TrPTP2 is detected as a ~65 kDa protein associated with the parasite's flagellum. Given that the flagellum is an important structure for cell differentiation in trypanosomatids, the presence of a protein responsible for tyrosine dephosphorylation in the T. rangeli flagellum could represent an interesting mechanism of regulation in this structure.
authors.affiliation: Laboratórios de Protozoologia e de Bioinformática, Departamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.
authors.forename: Elisa Beatriz
authors.initials: EB
authors.lastname: Prestes
authors.forename: Ethel
authors.initials: E
authors.lastname: Bayer-Santos
authors.forename: Patrícia
authors.initials: P
authors.lastname: Hermes Stoco
authors.forename: Thaís Cristine Marques
authors.initials: TC
authors.lastname: Sincero
authors.forename: Glauber
authors.initials: G
authors.lastname: Wagner
authors.forename: Adriana
authors.initials: A
authors.lastname: Umaki
authors.forename: Stenio Perdigão
authors.initials: SP
authors.lastname: Fragoso
authors.forename: Juliano
authors.initials: J
authors.lastname: Bordignon
authors.forename: Mário
authors.initials: M
authors.lastname: Steindel
authors.forename: Edmundo Carlos
authors.initials: EC
authors.lastname: Grisard
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D005407:Flagella / Q000201:enzymology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010802:Phylogeny; D017027:Protein Tyrosine Phosphatases / Q000235:genetics / Q000378:metabolism*; D057509:Trypanosoma rangeli / Q000201:enzymology* / Q000235:genetics / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D017027:Protein Tyrosine Phosphatases