title: Direct immobilization of oxyamine-modified proteins from cell lysates.
abstract: Oxyamine-modified proteins can be efficiently and selectively immobilized on ketone-coated glass slides at neutral pH in short reaction times by direct treatment and spotting of protein expression lysates without prior purification.
authors.affiliation: Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, D-44227 Dortmund, Germany.
authors.forename: Long
authors.initials: L
authors.lastname: Yi
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Chen
authors.forename: Po-Chiao
authors.initials: PC
authors.lastname: Lin
authors.forename: Hendrik
authors.initials: H
authors.lastname: Schröder
authors.forename: Christof M
authors.initials: CM
authors.lastname: Niemeyer
authors.forename: Yao-Wen
authors.initials: YW
authors.lastname: Wu
authors.forename: Roger S
authors.initials: RS
authors.lastname: Goody
authors.forename: Gemma
authors.initials: G
authors.lastname: Triola
authors.forename: Herbert
authors.initials: H
authors.lastname: Waldmann
mesh_terms: D000588:Amines / Q000737:chemistry*; D004926:Escherichia coli; D005898:Glass; D007659:Ketones / Q000737:chemistry; D040081:Protein Array Analysis; D011506:Proteins / Q000737:chemistry*
chemical_list: D000588:Amines; D007659:Ketones; D011506:Proteins