title: [Tail-suspended model simulating mouse oocytes maturation inhibited with microgravity].
abstract: We studied the effects of simulated microgravity on mouse oocytes maturation, and analyzed whether the tail-suspended model can be applied to investigate simulated microgravity effects on reproductive processes in female mice. Mouse oocytes were cultured in vitro with microgravity simulated by a rotating wall vessel bioreactor and by tail-suspended model, and the maturation rate of the mouse oocytes in the two models were examined in vivo. The maturation rate of mouse oocytes cultured in simulated microgravity was 8.93%, and that was 72.33% in 1g gravity. In ratio, oocyte maturation rate had no significant difference between the rotational group and control group. Microgravity simulated by the tail-suspended model inhibited mouse oocytes maturation and increased the rate of oocytes abnormity. The maturation rate of tail-suspended mouse oocytes was 14.54%, which was significantly lower than that of control group. Tail-suspended model should be an ideal model to investigate simulated microgravity effects on reproductive processes of female mice.
authors.affiliation: College of Animal Science, South China Agriculture University, Guangzhou 510642, China.
authors.forename: Changli
authors.initials: C
authors.lastname: Wu
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Hengxi
authors.initials: H
authors.lastname: Wei
authors.forename: Zhenfang
authors.initials: Z
authors.lastname: Wu
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Jiang
authors.forename: Shouquan
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D019417:Hindlimb Suspension; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D009866:Oogenesis / Q000502:physiology*; D018474:Weightlessness Simulation