title: [Effect of curcumin on synapse-related protein expression of APP/PS1 double transgenic mice].
abstract: OBJECTIVE
  To observe the effect of curcumin on the expression of synapse-related proteins PSD-95 and Shank1 in APP/PS1 double transgenic mice.
  
  
  METHODS
  Three-month-old APP/PS1 dtg mice were randomly divided into the model group, the positive Rosiglitazone control group and curcumin high (400 mg x kg(-1) x d(-1)), medium (200 mg x kg(-1) x d(-1)) and low (100 mg x kg(-1) x d(-1)) dose groups, with non-genetically modified mice with the same background as the normal group. After the oral administration for three months, immunohistochemistry and Western blot were adopted for detection.
  
  
  RESULTS
  According to the behavioral detection, the treatment group and the model group showed differences in the place navigation test and the spatial probe test to varying degrees (P < 0.01 or P < 0.05). The expression of PSD-95 and Shank1-positive cells of hippocampus CA1 region significantly decreased in model mice compared with normal control group (P < 0.01); while the curcumin intervention group showed recovery to some extend. Western blot results showed that the strap of PSD-95 protein expression became significantly thinner and lighter in the model group compared with the normal control group (P < 0.01); while the curcumin intervention group showed notably thicker and darker straps of PSD-95 protein expression (P < 0.05).
  
  
  CONCLUSIONS
  Curcumin can increase the expression of synapse-related proteins PSD95 and Shank1 in APP/PS1 double transgenic mice, improve structure and plasticity of synapse in APP/PS1 double transgenic mice and enhance their learning and memory abilities.
authors.affiliation: Key Laboratory of Pharmacology, Dongzhinmen Hospital, Beijing University of Chinese Medicine, Class III Biosafety Lab of State Administration of Traditional Chinese Medicine, Beijing 100700, China. 472792896@qq.com
authors.forename: Peng
authors.initials: P
authors.lastname: Wei
authors.forename: Ruisheng
authors.initials: R
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Ren
authors.forename: Haiyun
authors.initials: H
authors.lastname: Sun
authors.forename: Jinduo
authors.initials: J
authors.lastname: Yang
authors.forename: Pengwen
authors.initials: P
authors.lastname: Wang
mesh_terms: D016564:Amyloid beta-Protein Precursor / Q000235:genetics*; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000378:metabolism; D003474:Curcumin / Q000494:pharmacology*; D000076122:Disks Large Homolog 4 Protein; D005786:Gene Expression Regulation / Q000187:drug effects*; D051528:Guanylate Kinases / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D053764:Presenilin-1 / Q000235:genetics*; D013569:Synapses / Q000187:drug effects / Q000378:metabolism*
chemical_list: D016564:Amyloid beta-Protein Precursor; D000076122:Disks Large Homolog 4 Protein; C501447:Dlg4 protein, mouse; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D053764:Presenilin-1; C512412:SHANK1 protein, mouse; D051528:Guanylate Kinases; D003474:Curcumin