title: Synthesis of post-translationally modified proteins.
abstract: Post-translational modifications of proteins can have dramatic effect on the function of proteins. Significant research effort has gone into understanding the effect of particular modifications on protein parameters. In the present paper, I review some of the recently developed tools for the synthesis of proteins modified with single post-translational modifications at specific sites in the protein, such as amber codon suppression technologies, tag and modify, and native chemical ligation.
authors.affiliation: Division of Cell Biology II, Chemical Biology Laboratory, B6, Netherlands Cancer Institute (NKI), Plesmanlaan 121, Amsterdam, 1066 CX, The Netherlands. s.v.kasteren@nki.nl
authors.forename: Sander
authors.initials: S
authors.lastname: van Kasteren
mesh_terms: D000818:Animals; D006801:Humans; D015394:Molecular Structure; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*
chemical_list: D011506:Proteins