title: Covalent bonding of GYIGSR to EVAL membrane surface to improve migration and adhesion of cultured neural stem/precursor cells.
abstract: In the present study, we modified poly (ethylene-co-vinyl alcohol) (EVAL) membranes with the covalent bonding of the laminin-derived peptides, GYIGSR by using carbodiimidazole (CDI) to activate the hydroxyl groups on the membrane surface. The resulting GYIGSR-immobilized EVAL (EVAL-GYIGSR) membrane was analyzed in terms of the effect of immobilized peptide sequence on the behaviors of neural stem/precursor cells (NSPCs), isolated from embryonic rat cerebral cortex, in the serum-free medium. Compared to the unmodified EVAL, GYIGSR immobilized on the EVAL membrane was shown to significantly increase NSPCs migrating out of neurospheres (p<0.05). In addition, NSPCs on the EVAL-GYIGSR membrane were able to differentiate into neural lineage cells and differentiated neurons expressed functional synaptic activity. Basically, there was no significant difference between GYIGSR-immobilized and laminin-coated substrates for their ability to enhance migration and differentiation of NSPCs, suggesting that the immobilization of GYIGSR on the EVAL membrane was successful and the laminin-derived peptide YIGSR and laminin had similar effect on NSPC behaviors. However, it is non-permanent modification for coating laminin on the substrates to support cell survival after a long-term culture. In this study, differentiated neurons could still adhere to the EVAL-GYIGSR surface with functional synaptic activity after incubation for 20 days. Therefore, the bioactive EVAL-GYIGSR provided an alternative approach to improve migration and survival of NSPCs for neural tissue engineering applications.
authors.affiliation: Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan, ROC.
authors.forename: Yi-Chen
authors.initials: YC
authors.lastname: Li
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Liao
authors.forename: Hsu-Hsien
authors.initials: HH
authors.lastname: Chang
authors.forename: Tai-Horng
authors.initials: TH
authors.lastname: Young
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D007797:Laminin / Q000737:chemistry; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects*; D009842:Oligopeptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D011145:Polyvinyls / Q000737:chemistry*; D011247:Pregnancy; D051381:Rats
chemical_list: D007797:Laminin; D009842:Oligopeptides; D011108:Polymers; D011145:Polyvinyls; C061514:glycyl-tyrosyl-isoleucyl-glycyl-seryl-arginine; C020320:ethylene-vinyl alcohol copolymer