title: Application of high-throughput IEF-PAGE for EPO-doping testing.
abstract: Since its first publication in 2000, the isoelectric focusing (IEF) method of erythropoietin (EPO) used in doping control has been considered a procedure with relatively small sample number capacity. To overcome this limitation, a variation of the current protocol was evaluated, which uses double-sized gels with 48-120 wells plus three electrodes and hence multiplies the capacity of the electrophoretic chamber. With this modification up to 120 samples and standards can be run on a single gel - thus, making IEF-PAGE of EPO a high-throughput method. The protocol is ideally suited for large-scale screening purposes.
authors.affiliation: Doping Control Laboratory, AIT Seibersdorf Laboratories, A-2444 Seibersdorf, Austria. christian.reichel@seibersdorf-laboratories.at
authors.forename: Christian
authors.initials: C
authors.lastname: Reichel
mesh_terms: D004300:Doping in Sports; D004867:Equipment Design; D004921:Erythropoietin / Q000032:analysis*; D057166:High-Throughput Screening Assays / Q000295:instrumentation*; D006801:Humans; D007525:Isoelectric Focusing / Q000295:instrumentation*; D015813:Substance Abuse Detection / Q000295:instrumentation*
chemical_list: D004921:Erythropoietin