title: Dietary tools to modulate glycogen storage in gilthead seabream muscle: glycerol supplementation.
abstract: The quality and shelf life of fish meat products depend on the skeletal muscle's energetic state at slaughter, as meat decomposition processes can be exacerbated by energy depletion. In this study, we tested dietary glycerol as a way of replenishing muscle glycogen reserves of farmed gilthead seabream. Two diets were tested in duplicate (n = 42/tank). Results show 5% inclusion of crude glycerol in gilthead seabream diets induces increased muscle glycogen, ATP levels and firmness, with no deleterious effects in terms of growth, proximate composition, fatty acid profile, oxidative state, and organoleptic properties (aroma and color). Proteomic analysis showed a low impact of glycerol-supplementation on muscle metabolism, with most changes probably reflecting increased stress coping capacity in glycerol-fed fish. This suggests inclusion of crude glycerol in gilthead seabream diets (particularly in the finishing phase) seems like a viable strategy to increase glycogen deposition in muscle without negatively impacting fish welfare and quality.
authors.affiliation: CIMAR/CCMAR, Centre of Marine Sciences of Algarve, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal. tome@tomesilva.com
authors.forename: Tomé S
authors.initials: TS
authors.lastname: Silva
authors.forename: Elisabete
authors.initials: E
authors.lastname: Matos
authors.forename: Odete D
authors.initials: OD
authors.lastname: Cordeiro
authors.forename: Rita
authors.initials: R
authors.lastname: Colen
authors.forename: Tune
authors.initials: T
authors.lastname: Wulff
authors.forename: Eduardo
authors.initials: E
authors.lastname: Sampaio
authors.forename: Vera
authors.initials: V
authors.lastname: Sousa
authors.forename: Luisa M P
authors.initials: LM
authors.lastname: Valente
authors.forename: Amparo
authors.initials: A
authors.lastname: Gonçalves
authors.forename: Joana M G
authors.initials: JM
authors.lastname: Silva
authors.forename: Narcisa
authors.initials: N
authors.lastname: Bandarra
authors.forename: Maria Leonor
authors.initials: ML
authors.lastname: Nunes
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Dinis
authors.forename: Jorge
authors.initials: J
authors.lastname: Dias
authors.forename: Flemming
authors.initials: F
authors.lastname: Jessen
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D003124:Colorimetry; D004032:Diet; D019587:Dietary Supplements; D005990:Glycerol / Q000008:administration & dosage*; D006003:Glycogen / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D021541:Sea Bream / Q000378:metabolism*
chemical_list: D006003:Glycogen; D005990:Glycerol