title: Molecular epidemiology of PRRSV from China's Guangxi Province between 2007 and 2009.
abstract: Porcine reproductive and respiratory syndrome (PRRS) is considered to be one of the most important infectious diseases affecting livestock. This study used gene sequence analysis of ORF5 and Nsp2 to determine the molecular epidemiology of PRRSV in different parts of the Guangxi province of China. These genes were selected due to their extensive variation within the genome. Out of 189 samples from animals suspected to have PRRS, 145 were PRRSV RNA positive. ORF5 and Nsp2 gene sequence analysis of 31 of these samples showed that all of the Guangxi isolates were of type 2. A phylogenetic tree analysis based on ORF5 showed that the Guangxi isolates were divided into two groups. Most of these were closely related to highly pathogenic strains, showing a 30 amino acid deletion at positions 481 and 533-561 of Nsp2, but an additional unique isolate (GXNN06) possessed a further four amino acid deletion at positions 485-488 of Nsp2.
authors.affiliation: College of Animal Sciences and Veterinary Medicine, Guangxi University, 100 Daxue Road, Nanning, Guangxi, China. wwzjlq123@163.com
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Zhang
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Liang
authors.forename: Xian-Ming
authors.initials: XM
authors.lastname: Meng
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Su
authors.forename: Hong-Pu
authors.initials: HP
authors.lastname: Zhang
authors.forename: Lin-Juan
authors.initials: LJ
authors.lastname: Xie
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: He
authors.forename: Yan-Sheng
authors.initials: YS
authors.lastname: Li
authors.forename: Shan
authors.initials: S
authors.lastname: Yin
authors.forename: Xiao-Quan
authors.initials: XQ
authors.lastname: Li
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Li
authors.forename: Ting Rong
authors.initials: TR
authors.lastname: Luo
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D005838:Genotype; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D013552:Swine; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins