title: A simplified purification method for AAV variant by polyethylene glycol aqueous two-phase partitioning.
abstract: Adeno-Associated Virus (AAV) has been widely used for in vivo study and preclinical therapy due to its ability to mediate long-term transgene expression, its lack of pathogenicity and low immunogenicity. It has been found that AAV has more than ten serotypes, which each transfect certain types of cells in the viral infected organ. Current methods for purification of different AAV serotypes utilize CsCl or Iodixanol ultrahigh speed density gradient centrifugation, which is expensive and time consuming. We recently developed a simplified method, PEG/(NH(4))(2)SO(4) aqueous two phase partitioning, for purification of AAV serotype 2 and 8. The method does not require ultrahigh speed gradient centrifugation or chromatography. Here we further explore the simplified method for purification of other serotypes of AAV, serotype 6 and 9. This simplified method not only can be used to purify serotype 2 and 8, but also serotype 6 and 9, indicating that a variety of AAV serotypes can be purified by this method.
authors.affiliation: Department of Surgery, Division of Pediatric Surgery, Children's Hospital of Pittsburgh, University of Pittsburgh, Pittsburgh, PA, USA. ping.guo@chp.edu
authors.forename: Ping
authors.initials: P
authors.lastname: Guo
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Xiao
authors.forename: Yousef
authors.initials: Y
authors.lastname: El-Gohary
authors.forename: Jose
authors.initials: J
authors.lastname: Paredes
authors.forename: Krishna
authors.initials: K
authors.lastname: Prasadan
authors.forename: Chiyo
authors.initials: C
authors.lastname: Shiota
authors.forename: John
authors.initials: J
authors.lastname: Wiersch
authors.forename: Carey
authors.initials: C
authors.lastname: Welsh
authors.forename: George K
authors.initials: GK
authors.lastname: Gittes
mesh_terms: D000645:Ammonium Sulfate / Q000737:chemistry; D002460:Cell Line; D002586:Cesium / Q000737:chemistry; D002712:Chlorides / Q000737:chemistry; D000229:Dependovirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry*
chemical_list: D002712:Chlorides; D002586:Cesium; D011092:Polyethylene Glycols; C028019:cesium chloride; D000645:Ammonium Sulfate