title: Delayed detection of cytomegalovirus-specific T-helper cells in a preterm infant following intrauterine exposure to tacrolimus.
abstract: After exposure to tacrolimus and CMV reactivation in utero, a preterm infant had a positive CMV polymerase chain reaction (PCR) in urine on day 2 of life. Naive T-cells were reduced, but stimulation with CMV antigen and a polyclonal T-cell activator in vitro yielded no measurable CD4+ T-cell response. When repeated on day 30, polyclonal activation was in the range of healthy adults, while the immune response to CMV was incomplete with a lack of IFNgamma+ CD4+ T-cells and no anti-CMV IgM. Thus, exposure to tacrolimus in utero caused a transitional T-cell activation defect which did not compromise viral clearance.
authors.affiliation: Department of Infectious Diseases, Virology, University of Heidelberg, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Von Au
authors.forename: Paul
authors.initials: P
authors.lastname: Schnitzler
authors.forename: Johannes
authors.initials: J
authors.lastname: Pöschl
authors.forename: Lutz
authors.initials: L
authors.lastname: Koch
authors.forename: Hans-Georg
authors.initials: HG
authors.lastname: Kräusslich
authors.forename: Thomas
authors.initials: T
authors.lastname: Böhler
mesh_terms: D001483:Base Sequence; D003587:Cytomegalovirus / Q000276:immunology*; D017931:DNA Primers; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D007231:Infant, Newborn; D007234:Infant, Premature; D018811:Maternal Exposure; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D016559:Tacrolimus / Q000627:therapeutic use*
chemical_list: D017931:DNA Primers; D007166:Immunosuppressive Agents; D016559:Tacrolimus