title: [Research on the expression of hemolysin genes of Leptospira in vivo by genechip].
abstract: OBJECTIVE
  To explore the expression of hemolysin genes of Leptospira in infected host.
  
  
  METHODS
  Amplified the gene segment of hemolysin genes from the genome of Leptospira by PCR for gene probe. Manufacture genechip by the VersArray Chipwriter systerm. The total RNAs of Leptospira before and after infection host were extracted, reversely transcribed to cDNA, after the random PCR, the products were marked with HEX and CY5 respectively, and hybridized to genechip to demonstrate the expression of hemolysin genes of Leptospira.
  
  
  RESULTS
  The hemolysin genes LA1029 (Ratio = 0.65), LA1027 (Ratio = 0.53) were up-regulated after infection of host; LA3540 (Ratio = 1.88), LA3937 (Ratio = 5.58), LA1029 (Ratio = 3.00) were up-regulated and LA4004 (Ratio = 0.67) was down-regulated in live than in blood; LA3937 (Ratio = 2.28), LA1029 (Ratio = 2.20) were up-regulated in kidney than in blood.
  
  
  CONCLUSIONS
  The expression level of hemolysin genes exist observable differences with inducement in vivo and in different organs. These suggested that these genes are probably involved in the pathogenesis and and disease progression.
authors.affiliation: Research Unit of Infection and Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Lang
authors.initials: L
authors.lastname: Bao
mesh_terms: D000818:Animals; D005260:Female; D006168:Guinea Pigs; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics*; D007919:Leptospira / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D007922:Leptospirosis / Q000382:microbiology*; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D006460:Hemolysin Proteins