title: The impact of UVB exposure and differentiation state of primary keratinocytes on their interaction with quantum dots.
abstract: In this study we utilised an in vitro model system to gain insight into the potential cellular interactions that quantum dot (QD) nanoparticles may experience while transiting the viable skin epidermis, and we consider the effects of UVB exposure. UVB skin exposure is known to induce a skin barrier defect that facilitates QD stratum corneum penetration. Primary human keratinocytes were cultured in low and high calcium to induce basal and differentiated phenotypes, respectively. Results suggest that differentiation state plays a role in keratinocyte response to UVB exposure and exposure to negatively charged CdSe/ZnS core/shell QD. QD cell uptake increased with QD dose but association with differentiated cells was significantly lower than the basal keratinocyte phenotype. Differentiated keratinocytes were also less sensitive to the cytotoxic effects of UVB exposure. We did not observe an effect of UVB preexposure on QD cytotoxicity level despite the fact that fluorescent microscopy and flow cytometry data suggest that UVB may slightly increase QD uptake in the basal cell phenotype. The implications of these findings for assessing potential risk of human skin exposure are discussed.
authors.affiliation: Department of Biomedical Engineering, University of Rochester , Rochester, NY , USA.
authors.forename: Luke J
authors.initials: LJ
authors.lastname: Mortensen
authors.forename: Supriya
authors.initials: S
authors.lastname: Ravichandran
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Delouise
mesh_terms: D002118:Calcium / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000528:radiation effects; D008856:Microscopy, Fluorescence; D061251:Primary Cell Culture; D045663:Quantum Dots / Q000633:toxicity*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D003470:Culture Media; D002118:Calcium