title: Quantification and identification of bacteria in acrylic resin dentures and dento-maxillary obturator-prostheses.
abstract: OBJECTIVE
  To quantify and identify bacteria detected in acrylic resin dentures and dento-maxillary obturator-prostheses after long-term use.
  
  
  METHODS
  The internal layer of denture bases from 13 daily-use removable acrylic resin dentures was sampled, while the inner fluid samples/no-fluid samples of obturators were collected from 11 in-use acrylic resin dento-maxillary obturator-prostheses. Samples were cultured, and isolated bacteria were counted and identified by molecular biological methods.
  
  
  RESULTS
  Bacteria were detected in five (38.5%) acrylic resin dentures and six (54.5%) acrylic resin obturators. Four Lactobacillus species and one Propionibacterium species were isolated from three repaired denture bases, and from two non-repaired dentures, two Actinomyces species and Streptococcus mutans were isolated. On the other hand, 17 bacterial species, belonging to the family and genera of Olsenella, Bacillus, Citrobacter, Enterobacteriaceae, Lactobacillus, Pantoea, Peptoniphilus, Klebsiella and Pseudomonas, were isolated from obturators. Several species of viable bacteria were detected in acrylic resin denture bases and obturators.
authors.affiliation: Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan. tak@m.tohoku.ac.jp
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: Kazuko
authors.initials: K
authors.lastname: Nakajo
authors.forename: Takuichi
authors.initials: T
authors.lastname: Sato
authors.forename: Shigeto
authors.initials: S
authors.lastname: Koyama
authors.forename: Keiichi
authors.initials: K
authors.lastname: Sasaki
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Takahashi
mesh_terms: D000180:Acrylic Resins; D001420:Bacteria, Aerobic / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003819:Denture Bases / Q000382:microbiology*; D003780:Denture Repair; D003824:Denture, Complete / Q000382:microbiology*; D006801:Humans; D058889:Molecular Typing; D010158:Palatal Obturators / Q000382:microbiology*
chemical_list: D000180:Acrylic Resins