title: Tethering factor P115: a new model for tether-SNARE interactions.
abstract: The membrane tethering factor p115 has been shown to have important functions in ER to Golgi traffic and Golgi biogenesis. The multidomain structure of p115 allows for interactions with a diverse array of proteins that govern cargo movement at the ER-Golgi interface. Within its C-terminal region p115 contains four coiled-coil domains (CC1-CC4). Of the four coiled-coils, only CC1 has been shown to be required for p115 function, presumably by its ability to bind numerous SNARE proteins as well as the small GTPase Rab1. Recently, we showed that CC4 also interacts with SNARE proteins and that CC4 is required for p115 function in Golgi homeostasis and the trafficking of transmembrane but not soluble cargo. Here, we propose a novel model wherein p115 facilitates membrane tethering and fusion by simultaneously engaging its CC1 and CC4 domains with distinct SNARE proteins to promote formation of SNARE complexes.
authors.affiliation: Department of Cell Biology, Developmental and Integrative; University of Alabama at Birmingham; Birmingham, AL USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Grabski
authors.affiliation: Division of Biological Sciences and Center for Structural and Functional Neuroscience; University of Montana; Missoula, MT USA.
authors.forename: Jesse
authors.initials: J
authors.lastname: Hay
authors.affiliation: Department of Cell Biology, Developmental and Integrative; University of Alabama at Birmingham; Birmingham, AL USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Sztul
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D033921:Vesicular Transport Proteins