title: Luteolibacter yonseiensis sp. nov., isolated from activated sludge using algal metabolites.
abstract: A Gram-negative, rod-shaped, aerobic bacterial strain, designated EBTL01(T), was isolated from activated sludge by using metabolites of microalgae Ankistrodesmus gracilis SAG278-2. Phylogenetic analyses based on 16S rRNA gene sequence showed that strain EBTL01(T) belongs to the family Verrucomicrobiaceae, class Verrucomicrobiae, and is related most closely to Luteolibacter pohnpeiensis A4T-83(T) (95.5 % sequence similarity) and Luteolibacter algae A5J-41-2(T) (95.2 %). The G+C content of the genomic DNA of strain EBTL01(T) was 56.3 mol% and the menaquinone MK-9 was detected as the predominant quinone. Major fatty acid components were iso-C14 : 0, C16 : 1ω7c and C16 : 0. The amino acids of the cell-wall peptidoglycan contained muramic acid and meso-diaminopimelic acid. These profile results supported the affiliation of strain EBTL01(T) to the genus Luteolibacter. On the other hand, based on chemotaxonomic properties and phenotypic characteristics, strain EBTL01(T) could be clearly differentiated from its phylogenetic neighbours. Therefore, strain EBTL01(T) represents a novel species of the genus Luteolibacter, for which the name Luteolibacter yonseiensis sp. nov. is proposed. The type strain is EBTL01(T) ( = KCTC 23678(T) = JCM 18052(T)).
authors.affiliation: WCU Center for Green Metagenomics, Yonsei University, Seoul, Republic of Korea.|School of Civil and Environmental Engineering, Yonsei University, Seoul, Republic of Korea.
authors.forename: Joonhong
authors.initials: J
authors.lastname: Park
authors.affiliation: School of Civil and Environmental Engineering, Yonsei University, Seoul, Republic of Korea.
authors.forename: Gyu Seok
authors.initials: GS
authors.lastname: Baek
authors.affiliation: WCU Center for Green Metagenomics, Yonsei University, Seoul, Republic of Korea.|School of Civil and Environmental Engineering, Yonsei University, Seoul, Republic of Korea.
authors.forename: Sung-Geun
authors.initials: SG
authors.lastname: Woo
authors.affiliation: WCU Center for Green Metagenomics, Yonsei University, Seoul, Republic of Korea.|School of Civil and Environmental Engineering, Yonsei University, Seoul, Republic of Korea.
authors.forename: Jangho
authors.initials: J
authors.lastname: Lee
authors.affiliation: WCU Center for Green Metagenomics, Yonsei University, Seoul, Republic of Korea.|School of Civil and Environmental Engineering, Yonsei University, Seoul, Republic of Korea.
authors.forename: Jihoon
authors.initials: J
authors.lastname: Yang
authors.affiliation: WCU Center for Green Metagenomics, Yonsei University, Seoul, Republic of Korea.|School of Civil and Environmental Engineering, Yonsei University, Seoul, Republic of Korea.
authors.forename: Juyoun
authors.initials: J
authors.lastname: Lee
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009112:Muramic Acids / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D061327:Verrucomicrobia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009112:Muramic Acids; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D024482:Vitamin K 2; C073355:menaquinone 9