title: Enhancing the production of Rhodobacter sphaeroides-derived physiologically active substances using carbonic anhydrase-immobilized electrospun nanofibers.
abstract: In order to achieve continuous biocatalytic hydration of CO(2), carbonic anhydrase (CA) derived from Rhodobacter sphaeroides was immobilized on electrospun polystyrene/poly(styrene-co-maleic anhydride) (PS/PSMA) nanofibers as cross-linked enzyme aggregates (CLEA). The CA-CLEA maintained more than 94.7% of its initial activity during 60 days of storage period at 4 °C and also retained more than 45.0% activity after 60 reuses. The capability of CA-CLEA to hydrolyze CO(2) was verified in terms of CaCO(3) precipitation. The immobilized CA induced enhanced cell growth of R. sphaeroides , which then increased the production of R. sphaeroides -derived organic substances including carotenoid, bacteriochlorophyll, porphyrin, and coenzyme Q10. Further exploitation of such nanofiber-catalyst complexes that allows environment friendly use of CO(2) is expected in a variety of research fields.
authors.affiliation: Departmet of Microbiology, Chungbuk National University, 52 Naesudong-Ro, Heungduk-Gu, Cheongju 361-763, South Korea.
authors.forename: Jae-Min
authors.initials: JM
authors.lastname: Park
authors.forename: Mina
authors.initials: M
authors.lastname: Kim
authors.forename: Hyun Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Am
authors.initials: A
authors.lastname: Jang
authors.forename: Jiho
authors.initials: J
authors.lastname: Min
authors.forename: Yang-Hoon
authors.initials: YH
authors.lastname: Kim
mesh_terms: D001429:Bacteriochlorophylls / Q000096:biosynthesis; D055162:Biocatalysis; D002245:Carbon Dioxide / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000378:metabolism*; D002338:Carotenoids / Q000096:biosynthesis; D004800:Enzymes, Immobilized / Q000378:metabolism; D008298:Maleates; D057139:Nanofibers / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D011166:Porphyrins / Q000096:biosynthesis; D012242:Rhodobacter sphaeroides / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000096:biosynthesis
chemical_list: D001429:Bacteriochlorophylls; D004800:Enzymes, Immobilized; D008298:Maleates; D011137:Polystyrenes; D011166:Porphyrins; C521900:poly(styrene-co-maleic anhydride); D014451:Ubiquinone; D002245:Carbon Dioxide; D002338:Carotenoids; D002256:Carbonic Anhydrases; C024989:coenzyme Q10