title: Actin filament motility induced variation of resonance frequency and rigidity of polymer surfaces studied by quartz crystal microbalance.
abstract: This contribution reports on the quantification of the parameters of the motility assays for actomyosin system using a quartz crystal microbalance (QCM). In particular, we report on the difference in the observed resonance frequency and dissipation of a quartz crystal when actin filaments are stationary as opposed to when they are motile. The changes in QCM measurements were studied for various polymer-coated surfaces functionalized with heavy meromyosin (HMM). The results of the QCM experiments show that the HMM-induced sliding velocity of actin filaments is modulated by a combination of the viscoelastic properties of the polymer layer including the HMM motors.
authors.affiliation: Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, United Kingdom.
authors.forename: Harm
authors.initials: H
authors.lastname: van Zalinge
authors.forename: Jenny
authors.initials: J
authors.lastname: Aveyard
authors.forename: Joanna
authors.initials: J
authors.lastname: Hajne
authors.forename: Malin
authors.initials: M
authors.lastname: Persson
authors.forename: Alf
authors.initials: A
authors.lastname: Mansson
authors.forename: Dan V
authors.initials: DV
authors.lastname: Nicolau
mesh_terms: D000199:Actins / Q000737:chemistry*; D015879:Myosin Subfragments / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D013499:Surface Properties
chemical_list: D000199:Actins; D015879:Myosin Subfragments; D011108:Polymers