title: Response of P. aeruginosa E(1) gene expression to cadmium stress.
abstract: Heavy metal resistance microorganism plays an important role on polluted soil bioremediation. To obtain further knowledge of the resistant mechanism employed by cadmium-resistant bacteria, some gene expression profiles at transcription level were investigated in P. aeruginosa E(1) subjected to cadmium stress using real-time PCR. Exposure to cadmium for 1 h, the expression of czcA, czcB, and czcC all reached the peak of up-regulation 8.82-, 4.83-, and 7.43-fold, respectively. The response of czcD was earlier and stronger than czcABC. CysM contributed to cysteine synthesis kept up-regulation within the beginning 2 h. The expression of mgtAE genes related to Mg(2+) influx was up-regulated all the while, znuB responsible for Zn(2+) transportation kept up-regulation from 30 min to 4 h. The result support the two cadmium-resistance mechanisms including effluxing and inactive the heavy metal ions. The mechanism was brought that increase of Mg(2+) and Zn(2+) in cytoplasm would prevent Cd(2+)-binding enzymes to decrease the harm to cell.
authors.affiliation: Hunan Key Lab of Green Packaging and Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, People's Republic of China. zengxiaoxi2003@163.com
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Zeng
authors.forename: Jianxin
authors.initials: J
authors.lastname: Tang
authors.forename: Xueduan
authors.initials: X
authors.lastname: Liu
authors.forename: Pei
authors.initials: P
authors.lastname: Jiang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002104:Cadmium / Q000378:metabolism / Q000494:pharmacology*; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D019216:Metals, Heavy / Q000378:metabolism / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D019216:Metals, Heavy; D002104:Cadmium