title: Excipient effects on humanized monoclonal antibody interactions with silicone oil emulsions.
abstract: The interfacial adsorption of three humanized monoclonal antibodies to emulsions of microdroplets of silicone oil was examined using indirect measurement via integrated peak areas in size-exclusion high-performance liquid chromatograms. The level of silicone oil far exceeded the typical levels used in prefillable syringes. The three antibodies rapidly adsorbed to silicone oil-water interfaces in various buffer formulations. Addition of 140 mM NaCl to solutions buffered with 10 mM l-histidine, pH 6.0, increased the amount of protein adsorbed. Conversely, the extent of adsorption was significantly decreased by the addition of 0.03% (w/v) Tween® 20. Stern-Volmer constants determined from intrinsic tryptophan fluorescence quenching by acrylamide suggested that the tertiary structure of the adsorbed antibodies was significantly perturbed. However, no aggregation or precipitation of the antibodies was detected. Flow cytometric analysis of emulsions of fluorescently stained silicone oil in solutions containing fluorescently labeled antibodies and light microscopy experiments suggested that agglomeration of silicone oil droplets in the emulsions occurred. Zeta potentials measured for silicone oil microdroplets with adsorbed antibodies suggested that droplet agglomeration was probably the result of reduced electrostatic energy barriers to droplet collisions.
authors.affiliation: Department of Chemical and Biological Engineering, University of Colorado at Boulder, Boulder, Colorado 80309, USA.
authors.forename: Keith A
authors.initials: KA
authors.lastname: Britt
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Schwartz
authors.forename: Christine
authors.initials: C
authors.lastname: Wurth
authors.forename: Hanns-Christian
authors.initials: HC
authors.lastname: Mahler
authors.forename: John F
authors.initials: JF
authors.lastname: Carpenter
authors.forename: Theodore W
authors.initials: TW
authors.lastname: Randolph
mesh_terms: D000327:Adsorption; D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry*; D006224:Cricetinae; D004655:Emulsions / Q000737:chemistry*; D005079:Excipients / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D051379:Mice; D011487:Protein Conformation; D012827:Silicone Oils / Q000737:chemistry*
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D004655:Emulsions; D005079:Excipients; D007074:Immunoglobulin G; D012827:Silicone Oils