title: [Construction of RNAi targeting TRAF1 gene and effect of TRAF1 on gastric cancer cells].
abstract: OBJECTIVE
  To construct the RNAi targeting tumor necrosis factor receptor associated factor (TRAF1) gene, and to explore the effect of interference targeting TRAF1 on the biological behavior of gastric cancer cells.
  
  
  METHODS
  We detected the expression of TRAF1 in BGC823, SGC7901, and MGC803 gastric cancer cell lines through the real-time PCR and Western blot; then we constructed three pLVXshRNA- TRAF1-shRNAs expression vector targeting TRAF1. When TRAF1 was interfered successfully, we selected the strongest interference efficiency ShRNA by real-time PCR and Western blot. Based on interference targeting TRAF1 on gastric cancer, we tested the cell proliferation activity and apoptosis through MTT assay and flow cytometry, and the cell migration by transwell migration assay.
  
  
  RESULTS
  The expression of TRAF1 was increased in BGC823, SGC7901, and MGC803 gastric cancer cell lines compared with gastric epithelial cells (P<0.05), and the highest expression was in BGC823 gastric cell line. In the three TRAF1 shRNAs, the strongest interference efficiency shRNA was pLVX-shRNA-TRAF1-shRNA2. When the gene TRAF1 of BGC823 was interfered, the cell growing power was weakened and the apoptosis rate increased, and the cell migration had no difference.
  
  
  CONCLUSIONS
  The expression of TRAF1 is up-regulated in gastric cancer cell lines BGC823, SGC7901, and MGC803, and the most obvious one is BGC823. The interference targeting TRAF1 can successfully inhibit the expression of TRAF1 in gastric cancer cell line BGC823. TRAF1 can inhibit the apoptosis of BGC823 cells.
authors.affiliation: Department of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Fen
authors.initials: F
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Qian
authors.initials: Q
authors.lastname: Feng
authors.forename: Guangkui
authors.initials: G
authors.lastname: Bu
authors.forename: Lihua
authors.initials: L
authors.lastname: Huang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Lu
authors.forename: Qin
authors.initials: Q
authors.lastname: Guo
authors.forename: Canxia
authors.initials: C
authors.lastname: Xu
authors.forename: Shourong
authors.initials: S
authors.lastname: Shen
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D045744:Cell Line, Tumor; D006801:Humans; D008969:Molecular Sequence Data; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D047990:TNF Receptor-Associated Factor 1 / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D015854:Up-Regulation / Q000235:genetics
chemical_list: D034741:RNA, Small Interfering; D047990:TNF Receptor-Associated Factor 1