title: Identification of two paralogous caprine CD36 genes that display highly divergent mRNA expression profiles.
abstract: The CD36 molecule plays a pivotal role in a variety of immunological and cellular processes, including pathogen recognition, inflammation and apoptosis. Herein, we demonstrate that this gene is duplicated in goats, with two copies (CD36 and CD36-like) that display highly divergent mRNA expression profiles. In this way, CD36 mRNA is mostly expressed in the adipose tissue and heart whilst CD36-like mRNA shows a high expression in the liver. We have also found evidence of the presence of two paralogous CD36 and CD36-like genes in the bovine genome, suggesting that CD36 duplication took place before goat-cattle radiation, i.e. at least 20 MYR ago. Finally, we have characterized the polymorphism of the coding regions of the goat CD36 and CD36-like genes. In doing so, we have identified one synonymous polymorphism at the CD36-like gene (c.390A>C) that displays a significant association (P=0.04) with milk somatic cell count, a parameter often used to diagnose mastitis in domestic ruminants.
authors.affiliation: Department of Animal Genetics, Center for Research in Agricultural Genomics, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Zidi
authors.forename: A
authors.initials: A
authors.lastname: Castelló
authors.forename: J
authors.initials: J
authors.lastname: Jordana
authors.forename: J
authors.initials: J
authors.lastname: Carrizosa
authors.forename: B
authors.initials: B
authors.lastname: Urrutia
authors.forename: J M
authors.initials: JM
authors.lastname: Serradilla
authors.forename: M
authors.initials: M
authors.lastname: Amills
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D018955:CD36 Antigens / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D005787:Gene Frequency; D056426:Genetic Loci; D016678:Genome; D005838:Genotype; D006041:Goats / Q000235:genetics / Q000378:metabolism; D008892:Milk / Q000166:cytology; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D018955:CD36 Antigens; D012333:RNA, Messenger