title: Production of sophorolipids with enhanced volumetric productivity by means of high cell density fermentation.
abstract: To achieve high time-space efficiency for sophorolipid production with yeast Candida bombicola, a strategy of high cell density fermentation was employed. The approach consisted of two sequential stages: (1) the optimization of the carbon source and the nutrient concentration to achieve the maximal cell density and (2) the computer-aided adjustment of physical parameters and the controlled feeding of substrates for enhanced volumetric productivity. Both stages have been successfully implemented in a 10-L fermenter, where up to 80 g dry cell weight/L was obtained and a remarkably high volumetric productivity (> 200 g isolated sophorolipids/L/day) was achieved. Both the biomass and volumetric productivity were markedly higher than previously reported. Specifically, the high productivity of sophorolipids could be attained on a very short time scale (24 h), highlighting the industrial potential of the platform developed in this work.
authors.affiliation: Department of Engineering, Aarhus University, 8000, Aarhus, Denmark.
authors.forename: Renjun
authors.initials: R
authors.lastname: Gao
authors.forename: Mia
authors.initials: M
authors.lastname: Falkeborg
authors.forename: Xuebing
authors.initials: X
authors.lastname: Xu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Guo
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002175:Candida / Q000254:growth & development / Q000378:metabolism*; D002452:Cell Count; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D050356:Lipid Metabolism; D013997:Time Factors
chemical_list: D003470:Culture Media