title: [Primary establishment of an alphaLISA assay for detection of HAV IgM].
abstract: OBJECTIVE
  To develop an AlphaLISA method for detection of antibody of Hepatitis A virus.
  
  
  METHODS
  After hepatitis A virus antigen was concentrated and biotinylated, optimal biotinylated HAV antigen, donor bead, acceptor bead concentration have been explored and determined by both dot-blotting and AlphaLISA methods. 97 samples including 23 serums from patients HAV infected and 70 serums from blood donors have been detected by new AlphaLISA method.
  
  
  RESULTS
  The sensitivity and specificity for anti-HAV IgM were 78% and 98.5%, the positive and negative predictive value were 95% and 93%.
  
  
  CONCLUSIONS
  A homogeneous and fast AlphaLISA assay for detection of HAV IgM has been established by preliminary verification on samples.
authors.affiliation: Wannan Medical College, Anhui 241000, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Gan
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.forename: Yu
authors.initials: Y
authors.lastname: Cui
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Chen
authors.initials: C
authors.lastname: Gao
authors.forename: Chao-Ping
authors.initials: CP
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Han
mesh_terms: D019921:Biotinylation; D006506:Hepatitis A / Q000175:diagnosis; D035922:Hepatitis A Antibodies / Q000097:blood*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D035922:Hepatitis A Antibodies; D007075:Immunoglobulin M