title: Tissue distribution of sialic acid-linked influenza virus receptors in beagle dogs.
abstract: Reports of influenza A virus infections in dogs has received considerable attention from veterinarians, virologists, and epidemiologists. Interaction between influenza viral hemagglutinin and cell oligosaccharides containing sialic acid residues results in infection. Sialic acids have an α-2,3-linkage to the penultimate galactose in the avian influenza virus receptor and an α-2,6-linkage in the human receptor. To date, there are no detailed data on the tissue distribution or histological features of either type of sialic acid-linked influenza virus receptors in beagle dogs, which are common laboratory animals and pets. We conducted the current study to visualize the in situ tissue distribution of both sialic acid-linked influenza virus receptors in various organs of beagle dogs using Maackia amurensis lectin II and Sambucus nigra agglutinin. Both α-2,3- and α-2,6-sialic acid-linked receptors were detected in the endothelial cells of the respiratory tract and other organs. Endothelial cells of most gastrointestinal organs were negative for α-2,3-sialic acid-linked receptors in the dogs. Our results suggested that these canine organs may be affected by influenza virus infection. The findings from our study will also help evaluate the occurrence and development of influenza virus infections in dogs.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Zhang-Yong
authors.initials: ZY
authors.lastname: Ning
authors.forename: Xin-Tao
authors.initials: XT
authors.lastname: Wu
authors.forename: Yan-Fen
authors.initials: YF
authors.lastname: Cheng
authors.forename: Wen-Bao
authors.initials: WB
authors.lastname: Qi
authors.forename: Yu-Fu
authors.initials: YF
authors.lastname: An
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.forename: Gui-Hong
authors.initials: GH
authors.lastname: Zhang
authors.forename: Shou-Jun
authors.initials: SJ
authors.lastname: Li
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000378:metabolism; D004285:Dogs / Q000378:metabolism / Q000821:virology*; D005260:Female; D053124:Influenza A Virus, H5N1 Subtype / Q000378:metabolism*; D029901:Maackia / Q000737:chemistry; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009928:Organ Specificity; D009976:Orthomyxoviridae Infections / Q000378:metabolism / Q000635:transmission / Q000662:veterinary; D037121:Plant Lectins / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D011991:Receptors, Virus / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D027924:Sambucus nigra / Q000737:chemistry
chemical_list: D037121:Plant Lectins; D011956:Receptors, Cell Surface; D011991:Receptors, Virus; C092488:sialic acid receptor; D019158:N-Acetylneuraminic Acid