title: [Fluid suspension co-culture of Dendrobium officinale protocorm and living fungus].
abstract: OBJECTIVE
  To set up a system for fluid suspension co-culture of Dendrobium officinale protocorm and living fungus MF24, so as to provide certain scientific evidence for industrial production of protocorm.
  
  
  METHODS
  Whether the protocorm culture system was suitable for the normal growth of MF24 fungus were studied, the growth of protocorm cultured alone and co-cultured with the fungus were researched under light and dark culture conditions, the biomass and proliferation times were determined, and HPLC method was used to analyze and compare the changes of 11 characteristic peak areas in D. officinale protocorm.
  
  
  RESULTS
  The MF24 fungus could grow normally in the 6,7-V liquid medium used to culture the protocorm, and when it was cultured by 8-10 hours per day under 1 500 lx, the growth rate of the fungus was slowed. Protocorm could grow normally in light and dark culture conditions, and add the MF24 fungus in the early cultivation stages of protocorm, both inhibit the growth of each other. In the protocorm for the growth stability to add 5 diameter 9 mm fungi block, the protocorm growth and chemical composition type had no significant effect. However, under illumination, co-cultured for 5 days protocorm of which 10 compounds content decreased 13.64% to 138.47%, in dark conditions, co-cultured for 5 days protocorm of which 7 compounds increased by 0.71% to 12.82%, and 4 compounds slightly reduced by 3.03% to 14. 14% compared with the control.
  
  
  CONCLUSIONS
  Under the appropriate condition, living fungus MF24 could co-culture with the D. officinale protocorm, and affected the latter's secondary metabolite levels.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
authors.forename: Zhixia
authors.initials: Z
authors.lastname: Meng
authors.forename: Ying
authors.initials: Y
authors.lastname: Shu
authors.forename: Chunlan
authors.initials: C
authors.lastname: Wang
authors.forename: Shunxing
authors.initials: S
authors.lastname: Guo
mesh_terms: D018920:Coculture Techniques / Q000379:methods*; D003624:Darkness; D031667:Dendrobium / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects; D005658:Fungi / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects; D013535:Suspensions
chemical_list: D013535:Suspensions