title: [Study on detoxication of kansui radix on normal liver cells LO2 after stir-baking with vinegar].
abstract: OBJECTIVE
  To compare the toxicity on normal liver cells LO2 before and after Kansui Radix stir-baked with vinegar, and make a preliminary study on the mechanism of detoxication of Kansui Radix stir-baked with vinegar.
  
  
  METHODS
  The MTT method was adopted to detect the cell activity, with normal liver cells LO2 as the study object. The morphology of cells were observed, and the level or content of AST, ALT, LDH, SOD, Na+-K+-ATPase, Ca2+-Mg2+ -ATPase, GSH and MDA were determined in cell culture supernatant and splitting supernatant.
  
  
  RESULTS
  Compared with the control group, Kansui can obviously inhibit the cell activity (P < 0.01) and morphology, and increase the levels of ALT, AST, and LDH (P < 0.01) in the supernatant fluid of cell incubation, and decrease the level of SOD and the content of GSH (P < 0.01). Besides, it significantly increased the content of MDA (P < 0.01) and significantly decreased the level of Na+-K+-ATPase and Ca2+-Mg2+ -ATPase (P < 0.01) in the supernatant fluid of cell dissociation. Compared with Kansui group of various doses, Kansui Radix stir-baked with vinegar can significantly decrease the cell proliferation inhibition and the trend of morphological variation, and obviously decrease the levels of ALT, AST, and LDH (P < 0.01) in the supernatant fluid of cell incubation, and significantly increase the level of SOD and the content of GSH (P < 0.01), and significantly decrease the content of MDA (P < 0.01). Additionally, it significantly increased the level of Na+-K+-ATPase and Ca2+-Mg2+ ATPase (P < 0.01) in the supernatant fluid of cell dissociation, and showed a certain dose-effect relationship.
  
  
  CONCLUSIONS
  Stir-baking with rice vinegar can release the hepatotoxicity of Kansui Radix. Its possible mechanism was that Kansui Radix stir-baked with vinegar can decrease the influence of Kansui Radix on the permeability of liver cells LO2 membrane and oxidative damage, in order to provide basis for further exploration of the detoxication mechanism of Kansui Radix stir-baked with vinegar.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China. yanxiaojing963@163.com
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yan
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Yudan
authors.initials: Y
authors.lastname: Cao
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Li
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Anwei
authors.initials: A
authors.lastname: Ding
mesh_terms: D019342:Acetic Acid / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000633:toxicity*; D028481:Euphorbia / Q000737:chemistry*; D006801:Humans; D008099:Liver / Q000166:cytology*; D018384:Oxidative Stress / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D000254:Sodium-Potassium-Exchanging ATPase; D019342:Acetic Acid