title: Elevated level of human RPA interacting protein α (hRIPα) in cervical tumor cells is involved in cell proliferation through regulating RPA transport.
abstract: Replication protein A (RPA) is a eukaryotic single-stranded DNA binding protein that is essential for DNA replication, repair, and recombination, and human RPA interacting protein α (hRIPα) is the nuclear transporter of RPA. Here, we report the regulatory role of hRIPα protein in cell proliferation. Western blot analysis revealed that the level of hRIPα was frequently elevated in cervical tumors tissues and hRIPα knockdown by siRNA inhibited cellular proliferation through deregulation of the cell cycle. In addition, overexpression of hRIPα resulted in increased clonogenicity. These results indicate that hRIPα is involved in cell proliferation through regulation of RPA transport.
authors.affiliation: Division of Biological Science and Technology, Yonsei University, Wonju 220-100, Republic of Korea.
authors.forename: Sim
authors.initials: S
authors.lastname: Namkoong
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Lee
authors.forename: Ik-Soon
authors.initials: IS
authors.lastname: Jang
authors.forename: Junsoo
authors.initials: J
authors.lastname: Park
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D004260:DNA Repair / Q000235:genetics; D005260:Female; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D021381:Protein Transport / Q000235:genetics; D051716:Replication Protein A / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
chemical_list: D002352:Carrier Proteins; C504742:RPA-interacting protein alpha, human; D051716:Replication Protein A