title: α-Fucosidases with different substrate specificities from two species of Fusarium.
abstract: Two fungal-secreted α-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on L-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-α-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan. A putative α-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was ~1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share <25 % overall amino acid identity. Alignment of all available fungal orthologs of FoFCO1 and FgFCO1 indicated that these two proteins belong to two subfamilies of fungal GH29 α-fucosidases. Fungal orthologs of subfamily 1 (to which FoFCO1 belongs) are taxonomically more widely distributed than subfamily 2 (FgFCO1), but neither was universally present in the sequenced fungal genomes. Trichoderma reesei and most species of Aspergillus lack genes for either GH29 subfamily.
authors.affiliation: Department of Energy (DOE) Plant Research Lab and DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Janet M
authors.initials: JM
authors.lastname: Paper
authors.forename: John S
authors.initials: JS
authors.lastname: Scott-Craig
authors.forename: David
authors.initials: D
authors.lastname: Cavalier
authors.forename: Ahmed
authors.initials: A
authors.lastname: Faik
authors.forename: Richard E
authors.initials: RE
authors.lastname: Wiemels
authors.forename: Melissa S
authors.initials: MS
authors.lastname: Borrusch
authors.forename: Mareike
authors.initials: M
authors.lastname: Bongers
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Walton
mesh_terms: D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics; D006027:Glycosides / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D005644:alpha-L-Fucosidase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D004271:DNA, Fungal; D006027:Glycosides; C014125:4-nitrophenylfucoside; D005644:alpha-L-Fucosidase