title: [Screening and verification on characteristic differentially expressed genes of Schistosoma japonicum from three reservoir hosts].
abstract: OBJECTIVE
  To get the characteristic differentially expressed genes of Schistosoma japonicum from three important reservoir hosts: yellow cattle, water buffalo and goat, so as to find the genetic markers to identify the various sources of the parasite reservoir hosts.
  
  
  METHODS
  The 49 d worms were collected from artificially infected animals, and the total RNA(s) of worms were extracted and reverse-transcripted to cDNA, and then hybridized with custom-built microarray to screen characteristic differentially expressed genes of every host, and the microarray results were validated by the real-time PCR method.
  
  
  RESULTS
  From results of microarray, we got 3 characteristic differentially expressed genes of S. japonicum from yellow cattle, 4 from water buffalo and 7 from goat. We verified schistosome samples from three reservoir hosts in another experiment, the results showed that 2 in yellow cattle, 3 in water buffalo, and 5 in goat were verified to be consistent with microarray results.
  
  
  CONCLUSIONS
  The ten characteristic differentially expressed genes of S. japonicum from three reservoir hosts screened by microarray might be used as genetic markers to identify the various sources of reservoir hosts for S. japonicum.
authors.affiliation: Shanghai Veterinary Research Institute, Key Laboratory for Animal Parasitology, Ministry of Agriculture, Chinese Academy of Agricultureal Sciences, Shanghai 200241, China.
authors.forename: Jian-Mei
authors.initials: JM
authors.lastname: Yang
authors.forename: Yao-Jun
authors.initials: YJ
authors.lastname: Shi
authors.forename: Xin-Gang
authors.initials: XG
authors.lastname: Feng
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Fu
authors.forename: Chun-Xiu
authors.initials: CX
authors.lastname: Yuan
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Hong
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Lu
authors.forename: Jiao-Jiao
authors.initials: JJ
authors.lastname: Lin
mesh_terms: D000818:Animals; D002417:Cattle; D004197:Disease Reservoirs / Q000469:parasitology*; D005260:Female; D020869:Gene Expression Profiling; D006041:Goats / Q000469:parasitology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012549:Schistosoma japonicum / Q000235:genetics* / Q000302:isolation & purification; D012554:Schistosomiasis japonica / Q000469:parasitology*