title: Glulathione-S-transferases gene polymorphism in prediction of gastric cancer risk by smoking and Helicobacter pylori infection status.
abstract: OBJECTIVE
  To evaluate the association of glutathione S-transferases gene polymorphisms with the risk of gastric cancer, with reference to smoking and Helicobacter pylori infection.
  
  
  METHODS
  We conducted a 1:1 matched case-control study with 410 gastric cancer cases and 410 cancer-free controls. Polymorphisms of GSTM1, GSTT1 and GSTP1 were determined using PCR-CTPP.
  
  
  RESULTS
  The GSTM1 and GSTT1 null genotypes were significantly associated with the risk of gastric cancer after adjusting for potential confounding factors (OR=1.68, 95% CI=1.32-2.23 for null GSTM1, OR=1.73; 95% CI=1.24-2.13 for null GSTT1). The combination of null GSTM1 and null GSTT1 conferred an elevated risk (OR=2.54, 95% CI=1.55-3.39). However, no association was found for GSTP1 polymorphism The smoking modified the association of GSTM1 and GSTT1 null genotypes with the risk of gastric cancer.
  
  
  CONCLUSIONS
  GSTM1 and GSTT1 null genotypes are associated with increased risk of gastric cancer, and smoking modifies the association.
authors.affiliation: Department of Cadre Ward, the General Hospital of Chengdu Military Area, Chengdu, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Jing
authors.forename: Zhi-Jie
authors.initials: ZJ
authors.lastname: Huang
authors.forename: Yu-Qin
authors.initials: YQ
authors.lastname: Duan
authors.forename: Pei-Hong
authors.initials: PH
authors.lastname: Wang
authors.forename: Ru
authors.initials: R
authors.lastname: Zhang
authors.forename: Ke-Shu
authors.initials: KS
authors.lastname: Luo
authors.forename: Xin-Rong
authors.initials: XR
authors.lastname: Xiao
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics*; D016481:Helicobacter Infections / Q000150:complications / Q000201:enzymology / Q000235:genetics*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D012306:Risk; D012907:Smoking / Q000009:adverse effects / Q000235:genetics*; D013274:Stomach Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology*
chemical_list: D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human