title: Hyperbaric oxygen therapy alleviates oxidative stress and tissue injury in renal ischemia/reperfusion injury in rats.
abstract: Hyperbaric oxygen (HBO) therapy has been shown to attenuate renal ischemia/reperfusion (I/R) injury in rats, when applied in the early reperfusion period. The aim of this study was to elucidate possible beneficial effects of HBO therapy on renal I/R injury, when applied 24 h after ischemia. Rats were randomized into three groups: (1) control group (n = 20), (2) I/R group (n = 20), and (3) I/R + HBO group (n = 20). Renal I/R injury was created by interrupting renal blood flow for 30 min with a non-traumatic vascular clamp. HBO therapy was administered 24 h after I/R injury and continued for 5 days. At the end of the study, rats were sacrificed under anesthesia, blood was drawn, and right kidneys were harvested for analysis. Renal I/R injury increased serum and tissue malondialdehyde (MDA) levels and reduced superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels. HBO therapy attenuated MDA levels by increasing SOD and GPx activities. HBO therapy also prevented neutrophil infiltration and tissue injury in kidneys. Taken together, HBO therapy has been found to be effective in the delayed period of I/R injury.
authors.affiliation: Department of Emergency Medicine, Gulhane Military Medical Academy, Ankara, Turkey.
authors.forename: Haluk
authors.initials: H
authors.lastname: Ilhan
authors.forename: Murat
authors.initials: M
authors.lastname: Eroglu
authors.forename: Volkan
authors.initials: V
authors.lastname: Inal
authors.forename: Yusuf Emrah
authors.initials: YE
authors.lastname: Eyi
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Arziman
authors.forename: Ali Osman
authors.initials: AO
authors.lastname: Yildirim
authors.forename: Ali
authors.initials: A
authors.lastname: Tansel
authors.forename: Günalp
authors.initials: G
authors.lastname: Uzun
authors.forename: Levent
authors.initials: L
authors.lastname: Yamanel
mesh_terms: D000818:Animals; D006931:Hyperbaric Oxygenation; D007668:Kidney / Q000098:blood supply*; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000628:therapy*