title: Analysis of the WISP3 gene in Indian families with progressive pseudorheumatoid dysplasia.
abstract: Progressive pseudorheumatoid dysplasia (PPD) is a progressive skeletal syndrome characterized by stiffness, swelling and pain in multiple joints with associated osteoporosis in affected patients. Radiographically, the predominant features resemble a spondyloepiphyseal dysplasia. Mutations in the WISP3 gene are known to cause this autosomal recessive condition. To date, only a limited number of studies have looked into the spectrum of mutations causing PPD. We report on clinical features and WISP3 mutations in a large series of Indian patients with this rare skeletal dysplasia. Families with at least one member showing clinical and radiologic features of PPD were recruited for the study. Symptoms, signs and radiographic findings were documented in 35 patients from 25 unrelated families. Swelling of small joints of hands and contractures are the most common presenting features. Mutation analysis was carried out by bidirectional sequencing of the WISP3 gene in all 35 patients. We summarize the clinical features of 35 patients with PPD and report on 11 different homozygous mutations and one instance of compound heterozygosity. Eight (c.233G>A, c.340T>C, c.348C>A, c.433T>C, c.682T>C, c.802T>G, c.947_951delAATTT, and c.1010G>A) are novel mutations and three (c.156C>A, c.248G>A, and c.739_740delTG) have been reported previously. One missense mutation (c.1010G>A; p.Cys337Tyr) appears to be the most common in our population being seen in 10 unrelated families. This is the largest cohort of patients with PPD in the literature and the first report from India on mutation analysis of WISP3. We also review all the mutations reported in WISP3 till date.
authors.affiliation: Diagnostics Division, Centre for DNA Fingerprinting and Diagnostics, Hyderabad, Andhra Pradesh, India.
authors.forename: Ashwin
authors.initials: A
authors.lastname: Dalal
authors.forename: Sri Lakshmi
authors.initials: SL
authors.lastname: Bhavani G
authors.forename: Padma Priya
authors.initials: PP
authors.lastname: Togarrati
authors.forename: Tatjana
authors.initials: T
authors.lastname: Bierhals
authors.forename: Madhusudan R
authors.initials: MR
authors.lastname: Nandineni
authors.forename: Sumita
authors.initials: S
authors.lastname: Danda
authors.forename: Debashish
authors.initials: D
authors.lastname: Danda
authors.forename: Hitesh
authors.initials: H
authors.lastname: Shah
authors.forename: Sandeep
authors.initials: S
authors.lastname: Vijayan
authors.forename: Kalpana
authors.initials: K
authors.lastname: Gowrishankar
authors.forename: Shubha R
authors.initials: SR
authors.lastname: Phadke
authors.forename: Abdul Mueed
authors.initials: AM
authors.lastname: Bidchol
authors.forename: Anand Prahalad
authors.initials: AP
authors.lastname: Rao
authors.forename: Sheela
authors.initials: S
authors.lastname: Nampoothiri
authors.forename: Kerstin
authors.initials: K
authors.lastname: Kutsche
authors.forename: K M
authors.initials: KM
authors.lastname: Girisha
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D001177:Arthropathy, Neurogenic / Q000000981:diagnostic imaging / Q000235:genetics*; D001483:Base Sequence; D055511:CCN Intercellular Signaling Proteins / Q000737:chemistry / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D003241:Consanguinity; D005190:Family; D005260:Female; D023061:Gene Order; D006801:Humans; D007194:India; D007223:Infant; D007592:Joint Diseases / Q000151:congenital; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D010375:Pedigree; D011859:Radiography; D016415:Sequence Alignment; D044465:White People / Q000235:genetics*; D055815:Young Adult
chemical_list: D055511:CCN Intercellular Signaling Proteins; C400081:CCN6 protein, human