title: Improved biocompatibility of poly (styrene-b-(ethylene-co-butylene)-b-styrene) elastomer by a surface graft polymerization of hyaluronic acid.
abstract: Hyaluronic acid (HA) is an important component of extracellular matrix (ECM) in many tissues, providing a hemocompatible and supportive environment for cell growth. In this study, glycidyl methacrylate-hyaluronic acid (GMHA) was first synthesized and verified by proton nuclear magnetic resonance ((1)H NMR) spectroscopy. GMHA was then grafted to the surface of biomedical elastomer poly (styrene-b-(ethylene-co-butylene)-b-styrene) (SEBS) via an UV-initiated polymerization, monitored by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The further improvement of biocompatibility of the GMHA-modified SEBS films was assessed by platelet adhesion experiments and in vitro response of murine osteoblastic cell line MC-3T3-E1 with the virgin SEBS surface as the reference. It showed that the surface modification with HA strongly resisted platelet adhesion whereas improved cell-substrate interactions.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Li
authors.forename: Shifang
authors.initials: S
authors.lastname: Luan
authors.forename: Hengchong
authors.initials: H
authors.lastname: Shi
authors.forename: Huawei
authors.initials: H
authors.lastname: Yang
authors.forename: Lingjie
authors.initials: L
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Jin
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yin
authors.forename: Paola
authors.initials: P
authors.lastname: Stagnaro
mesh_terms: D000475:Alkenes / Q000737:chemistry*; D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D002460:Cell Line; D020724:Elastomers / Q000737:chemistry* / Q000633:toxicity; D004852:Epoxy Compounds / Q000737:chemistry; D005030:Ethylenes / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D056951:Photoelectron Spectroscopy; D058105:Polymerization; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity; D017550:Spectroscopy, Fourier Transform Infrared; D020058:Styrene / Q000737:chemistry*
chemical_list: D000475:Alkenes; D020724:Elastomers; D004852:Epoxy Compounds; D005030:Ethylenes; D008689:Methacrylates; D011108:Polymers; C558934:butylene; D020058:Styrene; D006820:Hyaluronic Acid; C036216:ethylene; C007870:glycidyl methacrylate