title: Efficacy of all-trans retinoid acid in preventing nickel induced cardiotoxicity in myocardial cells of rats.
abstract: Nickel, a metal commonly found in battery plants and welding factories, has potential cardiotoxicity, while all-trans retinoid acid (atRA) can promote cardiovascular repair and myocardial recovery. The purpose of this study was to investigate whether atRA could prevent cardiotoxicity induced by nickel both in vitro and in vivo. In the study, a rat myocardial cell line (H9c2) exposed to different concentrations of nickel chloride (NiCl(2)) displayed apoptotic features accompanied by reactive oxygen species generation. In addition, NiCl(2) also caused obvious apoptosis and systolic dysfunction in primary myocardial cells. Treatment with atRA efficiently attenuated the cytotoxicities triggered by NiCl(2) as it significantly mitigated ROS generation and decreased MAP kinases activity in NiCl(2)-treated cardiomyocytes. Additionally, NiCl(2) exposure caused obvious arrhythmia in Sprague-Dawley rats with the maximum tolerance dose of NiCl(2) between 2 and 3mg/kg. A combinational intragastric administration of 40mg/kg atRA can partially reverse NiCl(2)-induced arrhythmia in rats. Our results suggested that atRA might have therapeutic potential in alleviating the adverse effects of nickel on the cardiovascular system.
authors.affiliation: Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Siyue
authors.initials: S
authors.lastname: Lou
authors.forename: Like
authors.initials: L
authors.lastname: Zhong
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Yang
authors.forename: Tao
authors.initials: T
authors.lastname: Xue
authors.forename: Renhua
authors.initials: R
authors.lastname: Gai
authors.forename: Difeng
authors.initials: D
authors.lastname: Zhu
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Meidan
authors.initials: M
authors.lastname: Ying
authors.forename: Qiaojun
authors.initials: Q
authors.lastname: He
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001145:Arrhythmias, Cardiac / Q000139:chemically induced / Q000517:prevention & control; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D020935:MAP Kinase Signaling System / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D009532:Nickel / Q000633:toxicity*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D020011:Protective Agents; D017382:Reactive Oxygen Species; D014212:Tretinoin; C022838:nickel chloride; D009532:Nickel