title: No evidence of association between MBL2A/O polymorphisms and Mycobacterium tuberculosis infection in populations from the Brazilian Amazon region.
abstract: The present study investigated the prevalence of the polymorphisms in the exon 1 of the MBL2 gene in patients with tuberculosis at a hospital in northern Brazil, which is a regional reference for the treatment of the disease. The study group was composed of 167 patients with tuberculosis, 34 of which had the extra-pulmonary form of the disease, while the other 133 had the pulmonary type. The control group consists of 159 healthy individuals. Samples of DNA extracted from leucocytes were submitted to Polymerase Chain Reaction for the amplification of a 120-bp segment of exon 1 of the MBL2 gene. The distribution of allele and genotype frequencies varied little among the different groups, and it was not possible to establish any clear association between the variants of the MBL2 gene and the susceptibility to or clinical profile of tuberculosis infections in the population analyzed.
authors.affiliation: University Hospital João de Barros Barreto - HUJBB, Federal University of Para, Brazil.
authors.forename: Mauro S
authors.initials: MS
authors.lastname: Araújo
authors.forename: Ednelza S
authors.initials: ES
authors.lastname: Graça
authors.forename: Vânia N
authors.initials: VN
authors.lastname: Azevedo
authors.forename: Izaura
authors.initials: I
authors.lastname: Cayres-Vallinoto
authors.forename: Luiz Fernando A
authors.initials: LF
authors.lastname: Machado
authors.forename: Marluisa O G
authors.initials: MO
authors.lastname: Ishak
authors.forename: Ricardo
authors.initials: R
authors.lastname: Ishak
authors.forename: Antonio C R
authors.initials: AC
authors.lastname: Vallinoto
mesh_terms: D000483:Alleles; D001938:Brazil / Q000453:epidemiology; D016022:Case-Control Studies; D005091:Exons; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000145:classification / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000254:growth & development*; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics; D014376:Tuberculosis / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin; D020033:Protein Isoforms