title: Automated online pretreatment and cleanup recycle coupled with high-performance liquid chromatography-mass spectrometry for determination of deca-bromodiphenyl ether in human serum.
abstract: Automated online SPE-HPLC-MS was established for the determination of deca-bromodiphenyl ether in human serum. The online SPE with large volume injection was utilized to enhance the sensitivity. Online SPE with dilution line greatly decreased matrices effect, which enabled serum samples to be injected directly into pre-column. Washing line was designed for the system to solve the serious residual phenomenon and reduce the risk of sample wastage and contamination. Under the optimized conditions, the linear of the method was in the range 0.1-10 ng/mL with the LOD of 0.026 ng/mL. The recoveries of serum samples spiked with deca-bromodiphenyl ether at 0.5 ng/mL was in the range from 83.30 to 102.7% with RSD in interday less than 8.67%. The satisfactory results demonstrated that the method of online sample pretreatment and cleanup recycle were reliable for human serum analysis.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Lin
authors.forename: Hai-Fang
authors.initials: HF
authors.lastname: Li
authors.forename: Xiangwei
authors.initials: X
authors.lastname: He
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hashi
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Lin
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D055768:Halogenated Diphenyl Ethers / Q000097:blood*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D055768:Halogenated Diphenyl Ethers; D014874:Water Pollutants, Chemical