title: [Effect of CCH1 or MID1 gene disruption on drug tolerance and pathogenesis of Candida albicans].
abstract: The calcium gate encoded by CCH1 and MID1 genes is the main channel for external calcium absorption. As one of the important secondary messengers, the elevation of calcium concentration could activate some pathways to take part in various cell processes. In this study, we used CCH1 and MID1 mutant strains and also constructed their complementary strains to study the effect of drug tolerance and virulence of Candida albicans after CCH1 or MID1 deletion. By drug plate sensitivity assay and the broth microdilution method, we compared the changes between different strains. Moreover, we added calcium channel blocker and inhibitors to analyze the effect of calcium concentration on drug action. After the deletion of CCH1 or MID1 gene, the strain exhibited an obvious sensitivity to FLUC and ITRA, and the drug action was regulated by the calcium concentration. In a mouse model of intravenous infection, we found that attenuated virulence of cch1delta/delta or mid1delta/delta strain is specifically due to a loss of CCH1 or MID1 gene.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Guangqing
authors.initials: G
authors.lastname: Lu
authors.forename: Baopeng
authors.initials: B
authors.lastname: Yang
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.forename: Qilin
authors.initials: Q
authors.lastname: Yu
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.forename: Xinxin
authors.initials: X
authors.lastname: Cheng
authors.forename: Laijun
authors.initials: L
authors.lastname: Xing
authors.forename: Mingchun
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D019703:Calcineurin / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism*; D002176:Candida albicans / Q000187:drug effects / Q000235:genetics / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology; D025141:Drug Resistance, Fungal / Q000235:genetics*; D005260:Female; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D051379:Mice; D008813:Mice, Inbred ICR; D014774:Virulence
chemical_list: D015220:Calcium Channels; D005656:Fungal Proteins; D019703:Calcineurin; D002118:Calcium