title: miRNA expression profile of colon cancer stem cells compared to non-stem             cells using the SW1116 cell line.
abstract: Colorectal cancer (CRC) is one of the major causes of cancer-related mortality             worldwide. Recent studies revealed that there is a relationship between CRC occurrence             and microRNA (miRNA) function. Stem cells are a type of cells that have the ability             to self-renew and to proliferate extensively while maintaining the undifferentiated             state. Cancer stem cells (CSCs) are closely linked to tumor recurrence and metastasis.             To this end, we evaluated the miRNA expression differences between colon CSCs             and non-stem cells using the SW1116 cell line, to determine the relationship between             tumor stem cells and tumor biological behavior. We isolated populations of colon             CSCs with the CD133+/CD44+ and CD133-/CD44- surface phenotype from a human SW1116             colon adenocarcinoma cell line using flow cytometry. The expression of miRNA and             mRNA of both sets of cells was examined with miRNA and mRNA arrays. Bioinformatic             methods were used to analyze microarray results. We completed gene ontology analysis,             pathway analysis, miRNA target gene prediction with databases. We identified a             colon stem cell miRNA expression profile comprising 31 upregulated and 31 downregulated             miRNAs, such as miR29a, miR29b, miR449b and miR4524. Some of these differentially             expressed miRNAs may be involved in the regulation of stem cell differentiation.             Gene ontology and pathway analyses showed that the differences are closely related             to the function of the cell cycle, cell differentiation, signaling pathway, cytoskeletal             proteins and cell-matrix adhesion in colon cancer stem cells. We found that miRNAs             play an important role in regulating the expression of colon CSC characteristics.             By regulating the expression of CSC signaling pathways, cytoskeleton and membrane             proteins, miRNAs give tumor stem cells the macrobiological behavior of recurrence             and metastasis. This study provides a new perspective on CRC metastasis and recurrence.
authors.affiliation: Department of General Surgery, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, PR China.
authors.forename: Yantian
authors.initials: Y
authors.lastname: Fang
authors.forename: Jianbin
authors.initials: J
authors.lastname: Xiang
authors.forename: Zongyou
authors.initials: Z
authors.lastname: Chen
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Gu
authors.forename: Zhengyang
authors.initials: Z
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Tang
authors.forename: Zhongwen
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000071916:AC133 Antigen; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000235:genetics* / Q000378:metabolism; D003599:Cytoskeleton / Q000235:genetics; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D018960:Hyaluronan Receptors; D035683:MicroRNAs; C000606023:PROM1 protein, human; D010455:Peptides; D012333:RNA, Messenger