title: [Relevance of the expression of CTNNAL1 and the resistance of respiratory tract in rat with airway hyperresponsiveness].
abstract: OBJECTIVE
  To observe the relation between the expression of CTNNAL1 and the airway resistance in rats with airway hyperresponsiveness.
  
  
  METHODS
  Thirty Wister rats were randomly divided into 5 groups: a normal control group, a 2 d ozone attack group, a 4 d ozone attack group, a 6 d ozone attack group, and a 6 d ozone attack+2 d dexamethasone treatment group (6 rats in each group). The distribution of CTNNAL1 was observed by in situ hybridization; the expression of CTNNAL1 was detected by fluorescence quantitative RT-PCR; the airway resistance was detected in by Buxco pulmonary function analysis system; and the relevance of the expression of CTNNAL1 and the resistance of respiratory tract in rat with airway hyperresponsiveness were analyzed.
  
  
  RESULTS
  CTNNAL1 was distributed in bronchial epithelial cells, goblet cells, endothelial cells, and the alveolar wall. With the increase of the ozone attack, the expression of CTNNAL1 mRNA gradually reduced, the airway hyperresponsiveness was aggravated, and the airway resistance was increased.
  
  
  CONCLUSIONS
  During airway hyperresponse, the reduction of CTNNAL1 mRNA can increase the airway resistance. There is a negative correlation between the reduction of CTNNAL1 mRNA and the airway hyperresponsiveness. CTNNAL1 is an adhesion molecule related to airway hyperresponsiveness susceptibility.
authors.affiliation: Research Department of Applied Pathophysiology, Hunan Normal University, Changsha, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Qiuxia
authors.initials: Q
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Shen
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Xie
authors.forename: Jiansong
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000403:Airway Resistance; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000503:physiopathology; D008297:Male; D010126:Ozone; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D051178:alpha Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: C000604298:CTNNAL1 protein, rat; D012333:RNA, Messenger; D051178:alpha Catenin; D010126:Ozone