title: Surface coatings shape the protein corona of SPIONs with relevance to their application in vivo.
abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) have proved their use in many biomedical applications, such as drug delivery, hyperthermia, and MRI (magnetic resonance imaging) contrast agents. Due to their instability in fluids, several surface coatings have been used to both stabilize and tune the properties of these nanoparticles (NPs) according to their applications. These coatings will strongly modify their surface properties and influence their interaction with the environment proteins in a relevant biological medium with a clear impact on their function. It is well-accepted that a protein corona is immediately formed when nanoparticles come in contact with a biological milieu, and the emergent bionano interface represents the biological identity of the particles. Here, we investigate how a different coating on the same magnetic core can influence the protein corona composition and structure with clear relevance to application of these NPs in medicine. In particular, we have studied the structure and composition of the protein corona-SPION complexes of magnetite nanoparticles stabilized with citric acid, poly(acrylic acid), or double layer oleic acid by a range of approaches, including dynamic light scattering, nanoparticle tracking analysis, differential centrifugal sedimentation, infrared spectroscopy, 1-D SDS gel electrophoresis, and mass spectroscopy.
authors.affiliation: Laboratory of Nanochemistry, Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary. angela.hajdu@net.sote.hu
authors.forename: Angéla
authors.initials: A
authors.lastname: Jedlovszky-Hajdú
authors.forename: Francesca Baldelli
authors.initials: FB
authors.lastname: Bombelli
authors.forename: Marco P
authors.initials: MP
authors.lastname: Monopoli
authors.forename: Etelka
authors.initials: E
authors.lastname: Tombácz
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Dawson
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D019343:Citric Acid / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D019301:Oleic Acid / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D011506:Proteins; C000499:ferric oxide; D019343:Citric Acid; D019301:Oleic Acid; C006903:carbopol 940