title: [Experimental evaluation of the Sysmex UF-1000i for ruling out non-gonococcal urethritis].
abstract: Acute nongonococcal urethritis (NGU) is one of the commonest sexually transmitted infections affecting men and women. The diagnosis of NGU has traditionally required microscopic evidence of urethritis. However, a significant proportion of patients with urethral symptoms do not have microscopic evidence of urethritis. The purpose of the present study was to evaluate the analytical performance of the UF1000i, a recently introduced fluorescence flow cytometer intended for urinalysis purposes which provides new analytical features that seem particularly suitable for microbiological diagnostics, for ruling out NGU or predicting the presence of infection. The Sysmex UF1000i is a flow cytometry analyzer capable of quantifying a lot of particles, including bacteria (BACT) and white blood cells (WBCs). To evaluate the analytical performance of the UF1000i as a method for ruling out NGU, we examined 200 urethral smear samples, collected in a new liquid transport medium (Copan), and compared the UF1000i results with standard culture/molecular and microscopic Gram stain results. With instrument cut-off values of 200 BACT x 10^6/L and 500 WBCs x 10^6/L, we obtained a sensitivity of 84%, a specificity of 82%, and a high negative predictive value (96%). Culture/molecular detection of pathogens remains the gold standard technique for the diagnosis of NGU. However, the Sysmex UF1000i is capable of improving the efficiency of NGU presumptive diagnosis, providing results in a few minutes, with a high negative predictive value and high values of sensitivity.
authors.affiliation: SOC di Microbiologia e Virologia, Azienda Ospedaliera S. Maria degli Angeli, Pordenone, Italy.
authors.forename: Shamanta
authors.initials: S
authors.lastname: Grosso
authors.forename: Graziano
authors.initials: G
authors.lastname: Bruschetta
authors.forename: Alessandro
authors.initials: A
authors.lastname: Camporese
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation*; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006069:Gonorrhea / Q000175:diagnosis; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D015231:Sexually Transmitted Diseases, Bacterial / Q000175:diagnosis* / Q000382:microbiology* / Q000652:urine; D013048:Specimen Handling; D014521:Urethra / Q000382:microbiology*; D014526:Urethritis / Q000175:diagnosis* / Q000382:microbiology* / Q000652:urine