title: Synergistic enhancement of cancer therapy using a combination of fusion protein MG7-scFv/SEB and tumor necrosis factor alpha.
abstract: The fusion protein MG7-scFv/SEB has shown anti-tumor activity on gastric cancer in vitro and in vivo. Tumor necrosis factor-alpha (TNF-α) is a cytokine exerting anti-tumor effectiveness in various models and modes of applications. In this study, we explored the combination effects of MG7-scFv/SEB and TNF-α in experimental gastric cancer. Both MG7-scFv/SEB and TNF-α could effectively result in a significant inhibition of tumor growth in our experimental models when administered alone. What's more, MG7-scFv/SEB synergized with TNF-α in further reducing the growth of gastric tumors in gastric-tumor-bearing rats as compared to mono therapy. Additionally, the survival rate of gastric-tumorbearing rats administrated with combined therapy was significantly higher than that of rats treated with MG7-scFv/SEB or TNF-α. These results indicate that combined therapy with MG7-scFv/SEB and TNF-α is a promising strategy for human cancer therapy.
authors.affiliation: Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Lu
authors.forename: Chao
authors.initials: C
authors.lastname: Cheng
authors.forename: Guobin
authors.initials: G
authors.lastname: Wang
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Shu
authors.forename: Jingwei
authors.initials: J
authors.lastname: Ma
authors.forename: Qiang
authors.initials: Q
authors.lastname: Tong
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D004768:Enterotoxins / Q000235:genetics*; D005260:Female; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics; D009336:Necrosis / Q000139:chemically induced; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000235:genetics / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000401:mortality; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D007128:Immunoglobulin Fragments; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; C031818:enterotoxin B, staphylococcal