title: A common Asn680Ser polymorphism in the follicle-stimulating hormone receptor gene is not associated with ovarian response to gonadotropin stimulation in patients undergoing in vitro fertilization.
abstract: OBJECTIVE
  To assess the role of the variant p.Asn680Ser in the follicle-stimulating hormone receptor (FSHR) gene in determining ovarian response in patients undergoing in vitro fertilization (IVF) treatment.
  
  
  METHODS
  Prospective observational study.
  
  
  METHODS
  Tertiary referral center for reproductive medicine.
  
  
  METHODS
  Women (n = 421) undergoing their first cycle of controlled ovarian stimulation for IVF and 83 healthy, ethnically matched controls.
  
  
  METHODS
  Baseline pelvic ultrasound and blood tests taken on days 2 to 3 of the cycle for assessment of baseline hormones and for DNA extraction.
  
  
  METHODS
  Genotypes for FSHR p.Asn680Ser determined using TaqMan allelic discrimination assay, and ovarian response to gonadotropin treatment classified as normal, poor, or overresponse based on the number of oocytes retrieved.
  
  
  RESULTS
  The FSHR p.Asn680Ser genotype frequencies were similar in IVF patients and controls. The number of oocytes retrieved was comparable between patients with different FSHR receptor genotypes. The total amount of gonadotropin used was also similar in all the genotype groups. A logistic regression analysis showed nonstatistically significant twofold difference in the distribution of genotypes between the groups with poor and normal ovarian response.
  
  
  CONCLUSIONS
  The variant FSHR p.Asn680Ser was not shown to be predictive of ovarian response, but clinically relevant differences cannot be ruled out.
authors.affiliation: Department of Reproductive Medicine, St. Mary's Hospital, University of Manchester, Manchester, United Kingdom.
authors.forename: Lamiya
authors.initials: L
authors.lastname: Mohiyiddeen
authors.affiliation: Genetic Medicine, Manchester Academic Health Sciences Centre (MAHSC), St. Mary's Hospital, University of Manchester, Manchester, United Kingdom.
authors.forename: William G
authors.initials: WG
authors.lastname: Newman
authors.affiliation: Genetic Medicine, Manchester Academic Health Sciences Centre (MAHSC), St. Mary's Hospital, University of Manchester, Manchester, United Kingdom.
authors.forename: Christian
authors.initials: C
authors.lastname: Cerra
authors.affiliation: Genetic Medicine, Manchester Academic Health Sciences Centre (MAHSC), St. Mary's Hospital, University of Manchester, Manchester, United Kingdom.
authors.forename: Helen
authors.initials: H
authors.lastname: McBurney
authors.affiliation: Department of Reproductive Medicine, St. Mary's Hospital, University of Manchester, Manchester, United Kingdom.
authors.forename: Betselot
authors.initials: B
authors.lastname: Mulugeta
authors.affiliation: Health Sciences-Methodology, University of Manchester, Manchester, United Kingdom.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Roberts
authors.affiliation: Maternal and Fetal Health Research Group, Manchester Academic Health Sciences Centre (MAHSC), St. Mary's Hospital, University of Manchester, Manchester, United Kingdom; GyneHealth, Manchester, United Kingdom. Electronic address: lnardo@gyne-health.co.uk.
authors.forename: Luciano G
authors.initials: LG
authors.lastname: Nardo
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005838:Genotype; D006062:Gonadotropins / Q000494:pharmacology*; D006801:Humans; D016015:Logistic Models; D054315:Oocyte Retrieval; D017063:Outcome Assessment, Health Care; D010053:Ovary / Q000166:cytology* / Q000187:drug effects*; D011110:Polymorphism, Genetic / Q000235:genetics*; D011446:Prospective Studies; D011962:Receptors, FSH / Q000235:genetics*; D062606:Tertiary Care Centers
chemical_list: D006062:Gonadotropins; D011962:Receptors, FSH