title: Multifunctional nanoparticles of Fe(3)O(4)@SiO(2)(FITC)/PAH conjugated the recombinant plasmid of pIRSE2-EGFP/VEGF(165) with dual functions for gene delivery and cellular imaging.
abstract: OBJECTIVE
  Technologies to increase tissue vascularity are critically important to the fields of tissue engineering and cardiovascular medicine. Angiogenic factors, like VEGF, have been widely investigated to induce vascular endothelial cell proliferation and angiogenesis for establishing a vascular network. However, effective transport of VEGF gene to target cells with minimal side effects remains a challenge despite the use of unique viral and non-viral delivery approaches.
  
  
  METHODS
  This study presents a novel gene delivery system of fluorescein isothiocyanate (FITC) doped and poly(allylamine hydrochloride) (PAH) grafted Fe(3)O(4)@SiO(2) nanoparticles, which allows efficient loading of pVEGF to form Fe(3)O(4)@SiO(2)(FITC)/PAH/pVEGF nanocomplexes for VEGF gene delivery and cellular imaging.
  
  
  RESULTS
  The nanocomplexes maintain their superparamagnetic property in the silica composites at room temperature, reaching a saturation magnetization value of 5.19 emu/g of material, and no appreciable change in magnetism even after PAH modification. The quantitative analysis of cellular internalization into the living human umbilical vein endothelial cells (HUVECs) demonstrated that the Fe(3)O(4)@SiO(2)(FITC)/PAH/pVEGF nanocomplexes could be entirely internalized by HUVECs, and exhibit high VEGF gene expression and an innocuous toxic profile. The magnetic resonance (MR) images showed that the superparamagnetic iron oxide core of Fe(3)O(4)@SiO(2)(FITC)/PAH/pVEGF nanocomplexes could also act as a contrast agent for MR imaging. This property provides a benefit for monitoring gene delivery.
  
  
  CONCLUSIONS
  These data highlight multifunctional Fe(3)O(4)@SiO(2)(FITC)/PAH/pVEGF nanocomplexes as an attractive platform for gene delivery of angiogenesis, and also making it a potential candidate of nanoprobes for cellular fluorescent imaging or MR imaging.
authors.affiliation: University of Electronic Science and Technology of China, School of Life Science and Technology, Department of Biophysics, Chengdu 610054, Sichuan, P.R. China. liuyiyao@hotmail.com
authors.forename: Yiyao
authors.initials: Y
authors.lastname: Liu
authors.forename: Mengran
authors.initials: M
authors.lastname: Shi
authors.forename: Mingming
authors.initials: M
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Chunhui
authors.initials: C
authors.lastname: Wu
mesh_terms: D002478:Cells, Cultured; D003287:Contrast Media; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D008279:Magnetic Resonance Imaging; D057054:Molecular Imaging; D053758:Nanoparticles; D010957:Plasmids; D011073:Polyamines / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes; D011073:Polyamines; D042461:Vascular Endothelial Growth Factor A; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C063994:polyallylamine; D012822:Silicon Dioxide; D016650:Fluorescein-5-isothiocyanate; D052203:Ferrosoferric Oxide