title: Dynamic microwave-assisted extraction coupled with salting-out liquid-liquid extraction for determination of steroid hormones in fish tissues.
abstract: In this work, a simple and fast sample pretreatment method was proposed for determination of steroid hormones in fish tissues by coupling dynamic microwave-assisted extraction with salting-out liquid-liquid extraction. The steroid hormones were successively extracted with acetonitrile and water under the action of microwave energy. Subsequently, the extract was separated into an acetonitrile phase and an aqueous phase with ammonium acetate. The acetonitrile phase containing the target analytes was concentrated and determined by LC-MS/MS. The limits of detection for the steroid hormones were in the range of 0.03-0.15 ng g(-1). This method was successfully applied to analyze seven kinds of fish tissues, and the recoveries of the steroid hormones for the spiked samples were in the range of 75.3 ± 4.9% to 95.4 ± 6.2%. Compared with the traditional method, the proposed method could reduce the consumption of the organic solvent, shorten the sample preparation time, and increase the sample throughput.
authors.affiliation: College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Zhou
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Chen
authors.forename: Guijie
authors.initials: G
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.forename: Weitao
authors.initials: W
authors.lastname: Song
authors.forename: Haiyan
authors.initials: H
authors.lastname: Jin
authors.forename: Lan
authors.initials: L
authors.lastname: Ding
mesh_terms: D000818:Animals; D017756:Aquaculture / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D005399:Fishes; D005506:Food Contamination / Q000032:analysis*; D012739:Gonadal Steroid Hormones / Q000032:analysis*; D059625:Liquid-Liquid Extraction; D008872:Microwaves; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis; D053719:Tandem Mass Spectrometry
chemical_list: D012739:Gonadal Steroid Hormones