title: Antimicrobial and anti-inflammatory activities of leaf extract of Valeriana wallichii DC.
abstract: Valeriana wallichii DC (Valerianaceae) is one of the most widely used traditional remedies for various complications associated with nervous system and digestion. No antimicrobial and anti-inflammatory studies have so far been carried out on the aerial parts of the plant. The present work was focused to evaluate the antimicrobial (antifungal and antibacterial) and anti-inflammatory properties of V. wallichii using reported methods. Chloroform fraction (VW-2) and hexane fraction (VW-3) exhibited significant activity against S. aureus and B. subtilus, respectively. The chloroform fraction (VW-2) showed significant activity against S. aureus with 0.27 mg/ml MIC, where 0.31 mg/ml MIC was deduced for VW-3 fraction against B. subtilus. VW-3 fraction was also found to be the most potent inhibitor of M. canis, showing 70% inhibition with an MIC value of 0.19 mg/ml. Considerable inhibitory activity was also observed for VW-2 and water fraction (VW-6) against M. canis and A. flavus. A remarkable anti-inflammatory like activity was observed for the crude extract at a dose of 200 mg/kg at all observed durations. Other doses of the sample also showed excellent activity. Looking to these results it may be concluded that V. wallichii may be a potential source for activity guided isolation of natural products with antimicrobial and anti-inflammatory-like properties.
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar, Pakistan. fazli_khuda2008@yahoo.com
authors.forename: Fazli
authors.initials: F
authors.lastname: Khuda
authors.forename: Zafar
authors.initials: Z
authors.lastname: Iqbal
authors.lastname: Zakiullah
authors.forename: Ayub
authors.initials: A
authors.lastname: Khan
authors.forename: Fazli
authors.initials: F
authors.lastname: Nasir
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001231:Aspergillus flavus / Q000187:drug effects / Q000254:growth & development; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D002351:Carrageenan; D002725:Chloroform / Q000737:chemistry; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control*; D016936:Gram-Positive Endospore-Forming Bacteria / Q000187:drug effects* / Q000254:growth & development; D006586:Hexanes / Q000737:chemistry; D008297:Male; D008826:Microbial Sensitivity Tests; D008864:Microsporum / Q000187:drug effects / Q000254:growth & development; D003904:Mitosporic Fungi / Q000187:drug effects* / Q000254:growth & development; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D014632:Valerian / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D000935:Antifungal Agents; D006586:Hexanes; D012997:Solvents; D002725:Chloroform; D002351:Carrageenan