title: The juxtamembrane domain of the E-cadherin cytoplasmic tail contributes to its interaction with Myosin VI.
abstract: We recently identified the atypical myosin, Myosin VI, as a component of epithelial cell-cell junctions that interacts with E-cadherin. Recombinant proteins bearing the cargo-binding domain of Myosin VI (Myo VI-CBD) or the cytoplasmic tail of E-cadherin can interact directly with one another. In this report we further investigate the molecular requirements of the interaction between Myo VI-CBD and E-cadherin combining truncation mutation analysis with in vitro binding assays. We report that a short (28 amino acid) juxtamembrane region of the cadherin cytoplasmic tail is sufficient to bind Myo VI-CBD. However, central regions of the cadherin tail adjacent to the juxtamembrane sequence also display binding activity for Myo VI-CBD. It is therefore possible that the cadherin tail bears two binding sites for Myosin VI, or an extended binding site that includes the juxtamembrane region. Nevertheless, our biochemical data highlight the capacity for the juxtamembrane region to interact with functionally-significant cytoplasmic proteins.
authors.affiliation: Division of Molecular Cell Biology; Institute for Molecular Bioscience; The University of Queensland, St. Lucia; Brisbane, QLD Australia.
authors.forename: Sabine
authors.initials: S
authors.lastname: Mangold
authors.affiliation: Division of Molecular Cell Biology; Institute for Molecular Bioscience; The University of Queensland, St. Lucia; Brisbane, QLD Australia.
authors.forename: Suzanne J
authors.initials: SJ
authors.lastname: Norwood
authors.affiliation: Division of Molecular Cell Biology; Institute for Molecular Bioscience; The University of Queensland, St. Lucia; Brisbane, QLD Australia.
authors.forename: Alpha S
authors.initials: AS
authors.lastname: Yap
authors.affiliation: Division of Molecular Cell Biology; Institute for Molecular Bioscience; The University of Queensland, St. Lucia; Brisbane, QLD Australia.
authors.forename: Brett M
authors.initials: BM
authors.lastname: Collins
mesh_terms: D015820:Cadherins / Q000737:chemistry* / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D006801:Humans; D018995:Myosin Heavy Chains / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D015820:Cadherins; C089785:myosin VI; D018995:Myosin Heavy Chains