title: Complexation of HIV derived peptides with carbosilane dendrimers.
abstract: Dendrimers have been proposed as new carriers for selected HIV-1 peptides. This paper reports on the complexation behaviour of the three HIV-derived-peptides: Gp160, NH-EIDNYTNTIYTLLEE-COOH; P24, NH-DTINEEAAEW-COOH and Nef, NHGMDDPEREVLEWRFDSRLAF-COOH with second generation cationic carbosilane dendrimers (CBD) branched with carbonsilicon bonds (CBD-CS) or oxygensilicon bonds (CBD-OS). Studies on the formation of complexes between HIV peptides and CBDs by fluorescence polarization, zeta-potential, electrophoresis and transmission electron microscopy have shown that both studied dendrimers form complexes with HIV peptides. At a molar ratio of (2.5-3):1 (dendrimer:peptide), the complexes formed were in the size range of 180-275 nm and with significant positive surface charge. The results suggest that interactions between dendrimers and HIV peptides have electrostatic nature due to the negative charge of peptides backbone and positive charge of dendrimer functional groups. Dendriplex stability depended on the type of studied dendrimers. Time of peptides release from the complexes ranged from 1 (CBD-OS) to ~36 (CBD-CS)h. Basing on the obtained results, we propose that the water-soluble cationic carbosilane dendrimers can be considered for delivery of HIV peptides to dendritic cells.
authors.affiliation: Department of General Biophysics, University of Lodz, Lodz, Poland. maksion@biol.uni.lodz.pl
authors.forename: Maksim
authors.initials: M
authors.lastname: Ionov
authors.forename: Karol
authors.initials: K
authors.lastname: Ciepluch
authors.forename: Barbara
authors.initials: B
authors.lastname: Klajnert
authors.forename: Sława
authors.initials: S
authors.lastname: Glińska
authors.forename: Rafael
authors.initials: R
authors.lastname: Gomez-Ramirez
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: de la Mata
authors.forename: Maria Angeles
authors.initials: MA
authors.lastname: Munoz-Fernandez
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
mesh_terms: D000595:Amino Acid Sequence; D050091:Dendrimers / Q000737:chemistry*; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005454:Fluorescence Polarization; D015497:HIV-1 / Q000737:chemistry*; D007202:Indicators and Reagents; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D010455:Peptides / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry*
chemical_list: D050091:Dendrimers; D007202:Indicators and Reagents; D010455:Peptides; D012821:Silanes; C504072:carbosilane