title: Low-copy nuclear primers and ycf1 primers in Cactaceae.
abstract: OBJECTIVE
  To increase the number of variable regions available for phylogenetic study in the Cactaceae, primers were developed for a portion of the plastid ycf1 gene and intron-spanning regions of two low-copy nuclear genes (isi1, nhx1). •
  
  
  RESULTS
  Primers were tested on several families within Caryophyllales, focusing on the Cactaceae. Gel electrophoresis indicated positive amplification in most samples. Sequences of these three regions (isi1, nhx1, ycf1) from Harrisia exhibited variation similar to or greater than two plastid regions (atpB-rbcL intergenic spacer and rpl16 intron). •
  
  
  CONCLUSIONS
  The isi, nhx, and ycf1 primers amplify phylogenetically useful information applicable to the Cactaceae and other families in the Caryophyllales.
authors.affiliation: Department of Cell Biology, Microbiology, and Molecular Biology, ISA 2015, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida 33620, USA. afranck@mail.usf.edu
authors.forename: Alan R
authors.initials: AR
authors.lastname: Franck
authors.forename: Bruce J
authors.initials: BJ
authors.lastname: Cochrane
authors.forename: James R
authors.initials: JR
authors.lastname: Garey
mesh_terms: D001483:Base Sequence; D029421:Cactaceae / Q000235:genetics*; D002467:Cell Nucleus / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism*; D018628:Gene Dosage / Q000235:genetics*; D017343:Genes, Plant / Q000235:genetics*
chemical_list: D017931:DNA Primers