title: Identification of VanN-type vancomycin resistance in an Enterococcus faecium isolate from chicken meat in Japan.
abstract: Five VanN-type vancomycin-resistant Enterococcus faecium strains were isolated from a sample of domestic chicken meat in Japan. All isolates showed low-level resistance to vancomycin (MIC, 12 mg/liter) and had the same pulsed-field gel electrophoresis profile. The vancomycin resistance was encoded on a large plasmid (160 kbp) and was expressed constitutively. The VanN-type resistance operon was identical to the first resistance operon to be reported, with the exception of a 1-bp deletion in vanT(N) and a 1-bp substitution in vanS(N).
authors.affiliation: Department of Bacteriology, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nomura
authors.forename: Koichi
authors.initials: K
authors.lastname: Tanimoto
authors.forename: Keigo
authors.initials: K
authors.lastname: Shibayama
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Arakawa
authors.forename: Shuhei
authors.initials: S
authors.lastname: Fujimoto
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Ike
authors.forename: Haruyoshi
authors.initials: H
authors.lastname: Tomita
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D017931:DNA Primers; D030541:Databases, Genetic; D016521:Electrophoresis, Gel, Pulsed-Field; D016984:Enterococcus faecium / Q000187:drug effects*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics; D007564:Japan; D008460:Meat / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009876:Operon; D010957:Plasmids / Q000235:genetics; D014640:Vancomycin; D020713:Vancomycin Resistance / Q000502:physiology*
chemical_list: D017931:DNA Primers; D014640:Vancomycin