title: [Differential expression of miR-21, miR-126, miR-143, miR-373 in normal cervical tissue, cervical cancer tissue and Hela cell].
abstract: OBJECTIVE
  To investigate the differential expression of miR-21, miR-126, miR-143 and miR-373 in normal cervical tissue, cervical cancer tissue and Hela cell.
  
  
  METHODS
  The expressions of miR-21, miR-126, miR-143 and miR-373 were detected by real-time PCR in cervical cancer tissue, cervical tissue of benign uterine tumor and Hela cell.
  
  
  RESULTS
  High expression of miR-21 was observed in cervical cancer and Hela cell, while low expression was observed in normal cervical tissue. The relative quantification of miR-21 in cerveical cancer was 11.3196 times that of miR-21 in normal cervical tissue (P < 0.05). The expression levels of miR-143 and miR-373 in cervical cancer and Hela cell were lower than those of normal cervical tissue. The relative quantification of miR-143 in cerveical cancer was 0.1553 times that of normal cervical tissue (P < 0.05), and the relative quantification of miR-373 in cerveical cancer was 0.4907 times that of normal cervical tissue (P < 0.05). The expression of miR-126 had no significant difference among cervical cancer tissue, Hela cell and normal cervical tissue (P > 0.05).
  
  
  CONCLUSIONS
  miRNAs are closely related to the occurrence and regulation of cervical cancer. The high expression of miR-21 in cervical cancer and Hela cell indicate that it may play a possible role of oncogenes, while miR-143 and miR-373 with low expression may play the role of tumor suppressor genes.
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Yue-Ling
authors.initials: YL
authors.lastname: Wang
authors.forename: Jiang-Fen
authors.initials: JF
authors.lastname: Wang
mesh_terms: D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C527111:MIRN126 microRNA, human; C542359:MIRN143 microRNA, human; C524940:MIRN21 microRNA, human; C527114:MIRN373 microRNA, human; D035683:MicroRNAs