title: Manipulation of gene expression in zebrafish using caged circular morpholino oligomers.
abstract: Morpholino oligomers (MOs) have been widely used to knock down specific genes in zebrafish, but their constitutive activities limit their experimental applications for studying a gene with multiple functions or within a gene network. We report herein a new design and synthesis of caged circular MOs (caged cMOs) with two ends linked by a photocleavable moiety. These caged cMOs were successfully used to photomodulate β-catenin-2 and no tail expression in zebrafish embryos.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38, Xueyuan Road, Beijing 100191, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Cong
authors.initials: C
authors.lastname: Lv
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Yang
authors.forename: Xinjing
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005326:Fetal Proteins; D005786:Gene Expression Regulation / Q000528:radiation effects; D060172:Morpholinos / Q000138:chemical synthesis / Q000737:chemistry* / Q000528:radiation effects; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010782:Photolysis; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism; D000098908:Brachyury Protein
chemical_list: D005326:Fetal Proteins; D060172:Morpholinos; D009838:Oligodeoxyribonucleotides; D020825:T-Box Domain Proteins; D029961:Zebrafish Proteins; D051176:beta Catenin; D000098908:Brachyury Protein