title: Direct visualization of cell division using high-resolution imaging of M-phase of the cell cycle.
abstract: Current approaches to monitor and quantify cell division in live cells, and reliably distinguish between acytokinesis and endoreduplication, are limited and complicate determination of stem cell pool identities. Here we overcome these limitations by generating an in vivo reporter system using the scaffolding protein anillin fused to enhanced green fluorescent protein, to provide high spatiotemporal resolution of mitotic phase. This approach visualizes cytokinesis and midbody formation as hallmarks of expansion of stem and somatic cells, and enables distinction from cell cycle variations. High-resolution microscopy in embryonic heart and brain tissues of enhanced green fluorescent protein-anillin transgenic mice allows live monitoring of cell division and quantitation of cell cycle kinetics. Analysis of cell division in hearts post injury shows that border zone cardiomyocytes in the infarct respond with increasing ploidy, but not cell division. Thus, the enhanced green fluorescent protein-anillin system enables monitoring and measurement of cell division in vivo and markedly simplifies in vitro analysis in fixed cells.
authors.affiliation: Institute of Physiology I, Life and Brain Center, University of Bonn, 53105 Bonn, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hesse
authors.forename: Alexandra
authors.initials: A
authors.lastname: Raulf
authors.forename: Gregor-Alexander
authors.initials: GA
authors.lastname: Pilz
authors.forename: Christian
authors.initials: C
authors.lastname: Haberlandt
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Klein
authors.forename: Ronald
authors.initials: R
authors.lastname: Jabs
authors.forename: Holm
authors.initials: H
authors.lastname: Zaehres
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Fügemann
authors.forename: Katrin
authors.initials: K
authors.lastname: Zimmermann
authors.forename: Jonel
authors.initials: J
authors.lastname: Trebicka
authors.forename: Armin
authors.initials: A
authors.lastname: Welz
authors.forename: Alexander
authors.initials: A
authors.lastname: Pfeifer
authors.forename: Wilhelm
authors.initials: W
authors.lastname: Röll
authors.forename: Michael I
authors.initials: MI
authors.lastname: Kotlikoff
authors.forename: Christian
authors.initials: C
authors.lastname: Steinhäuser
authors.forename: Magdalena
authors.initials: M
authors.lastname: Götz
authors.forename: Hans R
authors.initials: HR
authors.lastname: Schöler
authors.forename: Bernd K
authors.initials: BK
authors.lastname: Fleischmann
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D002455:Cell Division / Q000235:genetics / Q000502:physiology*; D003285:Contractile Proteins / Q000235:genetics / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006321:Heart / Q000196:embryology; D006801:Humans; D066298:In Vitro Techniques; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008938:Mitosis / Q000235:genetics / Q000502:physiology*; D009203:Myocardial Infarction / Q000235:genetics / Q000378:metabolism
chemical_list: D003285:Contractile Proteins; C095886:anillin; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins