title: Cold stratification and exogenous nitrates entail similar functional proteome adjustments during Arabidopsis seed dormancy release.
abstract: Despite having very similar initial pools of stored mRNAs and proteins in the dry state, mature Arabidopsis seeds can either proceed toward radicle protrusion or stay in a dormant state upon imbibition. Dormancy breaking, a prerequisite to germination completion, can be induced by different treatments though the underlying mechanisms remain elusive. Thus, we investigated the consequence of such treatments on the seed proteome. Two unrelated dormancy-releasing treatments were applied to dormant seeds, namely, cold stratification and exogenous nitrates, in combination with differential proteomic tools to highlight the specificities of the imbibed dormant state. The results reveal that both treatments lead to highly similar proteome adjustments. In the imbibed dormant state, enzymes involved in reserve mobilization are less accumulated and it appears that several energetically costly processes associated to seed germination and preparation for subsequent seedling establishment are repressed. Our data suggest that dormancy maintenance is associated to an abscisic-acid-dependent recapitulation of the late maturation program resulting in a higher potential to cope with environmental stresses. The comparison of the present results with previously published -omic data sets reinforces and extends the assumption that post-transcriptional, translational, and post-translational regulations are determinant for seed germination.
authors.affiliation: INRA, Jean-Pierre Bourgin Institute (IJPB, UMR1318 INRA-AgroParisTech), Laboratory of Excellence Saclay Plant Sciences, RD10, F-78002 Versailles Cedex, France. erwann.arc@versailles.inra.fr
authors.forename: Erwann
authors.initials: E
authors.lastname: Arc
authors.forename: Kamel
authors.initials: K
authors.lastname: Chibani
authors.forename: Philippe
authors.initials: P
authors.lastname: Grappin
authors.forename: Marc
authors.initials: M
authors.lastname: Jullien
authors.forename: Béatrice
authors.initials: B
authors.lastname: Godin
authors.forename: Gwendal
authors.initials: G
authors.lastname: Cueff
authors.forename: Benoit
authors.initials: B
authors.lastname: Valot
authors.forename: Thierry
authors.initials: T
authors.lastname: Balliau
authors.forename: Dominique
authors.initials: D
authors.lastname: Job
authors.forename: Loïc
authors.initials: L
authors.lastname: Rajjou
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism*; D003080:Cold Temperature; D015180:Electrophoresis, Gel, Two-Dimensional; D018525:Germination; D009566:Nitrates / Q000378:metabolism*; D020543:Proteome; D012639:Seeds / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D059467:Transcriptome
chemical_list: D029681:Arabidopsis Proteins; D009566:Nitrates; D020543:Proteome