title: Chemotactic behavior of pathogenic and nonpathogenic Leptospira species.
abstract: We have developed a capillary tube assay in combination with real-time PCR to quantitate the number of chemoattracted Leptospira cells. We identified Tween 80, glucose, sucrose, and pyruvate as attractants for Leptospira cells; amino acids and vitamin B(12) were found to be nonchemotactic or weakly chemotactic. This assay has the general applicability to further our understanding of leptospiral chemotaxis.
authors.affiliation: Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France.
authors.forename: Ambroise
authors.initials: A
authors.lastname: Lambert
authors.forename: Naoko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Nyles W
authors.initials: NW
authors.lastname: Charon
authors.forename: Mathieu
authors.initials: M
authors.lastname: Picardeau
mesh_terms: D058491:Bacterial Load; D002630:Chemotactic Factors / Q000032:analysis; D002633:Chemotaxis; D007919:Leptospira / Q000472:pathogenicity / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D002630:Chemotactic Factors