title: The use of sequential organ failure assessment parameters in an awake porcine model of severe Staphylococcus aureus sepsis.
abstract: The human sequential organ failure assessment (SOFA) scoring system is used worldwide in intensive care units for assessing the extent of organ dysfunction/failure in patients with severe sepsis. An increasing number of septic cases are caused by Gram-positive bacteria as Staphylococcus aureus. The aim of the current study was to apply the human SOFA parameters in an awake, porcine model of severe S. aureus sepsis. Five pigs were inoculated intravenously with S. aureus and two control animals were sham-inoculated. Extensive clinical monitoring and sequential blood sampling was obtained and analysed for SOFA parameters. Dysfunction/failure was observed in the respiratory, haemostatic and hepatic system of all infected animals, together with initial cardiovascular dysfunction. The pulmonary system was the first to fail clinically, which corresponds with similar human findings, whereas the liver was affected earlier in pigs compared to humans. The use of human SOFA parameters was valuable in identifying dysfunctional/failing organs and showed consistency between this porcine model and human severe sepsis. Applying SOFA parameters in this model increased the relevance for comparison to clinical methods of evaluating human severe sepsis. Changes in SOFA parameters may in future porcine studies serve as a target for monitoring the effect of therapeutic intervention.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark. elisabethsoerensen@gmail.com
authors.forename: Karen E
authors.initials: KE
authors.lastname: Soerensen
authors.forename: Ole L
authors.initials: OL
authors.lastname: Nielsen
authors.forename: Malene M
authors.initials: MM
authors.lastname: Birck
authors.forename: Dorte B
authors.initials: DB
authors.lastname: Soerensen
authors.forename: Páll S
authors.initials: PS
authors.lastname: Leifsson
authors.forename: Henrik E
authors.initials: HE
authors.lastname: Jensen
authors.forename: Bent
authors.initials: B
authors.lastname: Aalbaek
authors.forename: Annemarie T
authors.initials: AT
authors.lastname: Kristensen
authors.forename: Bo
authors.initials: B
authors.lastname: Wiinberg
authors.forename: Mads
authors.initials: M
authors.lastname: Kjelgaard-Hansen
authors.forename: Peter M H
authors.initials: PM
authors.lastname: Heegaard
authors.forename: Tine M
authors.initials: TM
authors.lastname: Iburg
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006487:Hemostasis; D009102:Multiple Organ Failure / Q000175:diagnosis / Q000503:physiopathology; D063305:Organ Dysfunction Scores; D018805:Sepsis / Q000175:diagnosis / Q000382:microbiology* / Q000503:physiopathology*; D013203:Staphylococcal Infections / Q000503:physiopathology*; D013552:Swine