title: Characterization of in vitro cultured bone marrow and adipose tissue-derived mesenchymal stem cells and their ability to express neurotrophic factors.
abstract: MSCs (mesenchymal stem cells) have attracted attention as a promising tool for regenerative medicine and transplantation therapy. MSCs exert neuroprotective effects by secreting a number of factors in vitro and in vivo. Similar characteristics are found in ADSCs (adipose-derived stem cells) and BMSCs (bone marrow stromal cells). Multipotent capability, easy accessibility and rapid proliferation of ADSCs have been established. Our main objective was to compare cell viability, growth rate, expression of neurotrophic factors and nestin genes in ADSCs and BMSCs. Cell doubling time and proliferation rate indicate that ADSCs has a higher proliferation rate than BMSCs. ADSCs and BMSCs express a similar pattern of CD71 and CD90 markers. Nestin immunostaining showed that ADSCs and BMSCs are immunopositive. The expression of neurotrophic factors genes in ADSCs proved similar to that of BMSCs genes. Thus adipose tissue stem cells with a high proliferation rate can express nestin and neurotrophic factor genes. Therefore ADSCs may be useful in future cell replacement therapies and help improve neurodegenerative diseases.
authors.affiliation: School of Biology, Damghan University, Damghan, Iran. ghorbanian@du.ac.ir
authors.forename: Ghorbanian Mohammad
authors.initials: GM
authors.lastname: Taghi
authors.forename: Haji
authors.initials: H
authors.lastname: Ghasem Kashani Maryam
authors.forename: Lashkarbolouki
authors.initials: L
authors.lastname: Taghi
authors.forename: Hosseinpour
authors.initials: H
authors.lastname: Leili
authors.forename: Mirzaeiyan
authors.initials: M
authors.lastname: Leyla
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D009414:Nerve Growth Factors / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D009414:Nerve Growth Factors