title: The Gastroprotective Role of Acanthus ilicifolius - A Study to Unravel the Underlying Mechanism of Anti-Ulcer Activity.
abstract: Acanthus ilicifolius (Acanthaceae), a mangrove medicinal plant, is widely used by the local inhabitants of the Sundarbans (India) to treat a variety of diseases. As a part of our continued search for novel bioactive products from mangrove medicinal plants, we were able to document the anti-inflammatory effects of this plant. In the present study, we have performed a detailed evaluation of the gastroprotective activity of the methanolic extract of Acanthus ilicifolius using different models of gastric ulceration. Unlike the conventional non-steroidal anti-inflammatory drugs, a methanolic extract of Acanthus ilicifolius leaves (MEAL) possessing significant anti-inflammatory properties, as revealed from our previous studies displayed in rats in dosages of 200 mg and 400 mg/kg BW after intraperitoneal administration, showed significant protective activity (anti-ulcer activity) against the gastric lesions induced by aspirin, indomethacin, stress, ethanol, and pylorus ligation. In pylorus-ligated rats, administration of Methanolic extract of Acanthus ilicifolius leaves (MEAL) significantly decreased gastric volume, acidity, and peptic activity. Moreover, pre-treatment with MEAL significantly restored the levels of reduced glutathione (GSH) and the antioxidant enzyme superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX), along with significant inhibition of both lipid peroxidation and myeloperoxidase (MPO) activity in pylorus-ligated animals. Ulceration induced with ethanol was significantly inhibited with MEAL, and the extract also resulted in the reduction of both lipid peroxidation and myeloperoxidase activity. Furthermore, in this experimental model, administration of MEAL improved the activities of SOD, CAT, GSH, and GPX. A similar pattern of action was also noticed in cold-restraint stress-induced (CRS) ulceration, where MEAL pre-treatment inhibited CRS-induced ulceration, improved the status of antioxidant enzymes, and also reduced the level of lipid peroxides. These results suggest that extracts of the leaves of Acanthus ilicifolius may exhibit anti-ulcer activities additional to the anti-inflammatory properties.
authors.affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
authors.forename: K T
authors.initials: KT
authors.lastname: Mani Senthil Kumar
authors.forename: Zothan
authors.initials: Z
authors.lastname: Puia
authors.forename: Samir K
authors.initials: SK
authors.lastname: Samanta
authors.forename: Rajiv
authors.initials: R
authors.lastname: Barik
authors.forename: Arnab
authors.initials: A
authors.lastname: Dutta
authors.forename: Bapi
authors.initials: B
authors.lastname: Gorain
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Roy
authors.forename: Dipan
authors.initials: D
authors.lastname: Adhikari
authors.forename: Sanmoy
authors.initials: S
authors.lastname: Karmakar
authors.forename: Tuhinadri
authors.initials: T
authors.lastname: Sen