title: Development of monoclonal antibodies to West Nile virus and their application in immunohistochemistry.
abstract: West Nile virus (WNV) is endemic throughout Africa, Eurasia, America, and Australia and has important implications for avian, horse, and human health. In these regions, dead birds are monitored for the presence of WNV through immunohistochemistry (IHC) and PCR. However, a number of the tools for IHC are inadequate owing to their cross-reactivity to other Japanese encephalitis serogroup viruses. Here we have established eight monoclonal antibodies (MAbs) to WNV. Four of them bound to the envelope protein, three of them bound to nonstructural protein 1 (NS1), and one bound to precursor membrane protein (prM), as shown by Western blot analysis. The anti-NS1 MAbs and the anti-prM MAb did not cross-react with Japanese encephalitis virus (JEV), Murray valley encephalitis virus, or St. Louis encephalitis virus in an indirect enzyme-linked immunosorbent assay. One NS1-specific MAb, SHW-32B1, and the previously reported NS1-specific MAb, SHW-7A11, were shown by IHC to specifically detect the cytoplasm of degenerated cells in the heart and brain of a WNV-infected goose. Neither of these MAbs were shown by IHC to cross-react with degenerated cells in the brain of a JEV-infected pig. These MAbs are the first reported anti-NS1 MAbs that can be used for WNV-specific IHC using formalin-fixed, paraffin-embedded sections. They may be useful for WNV research and surveillance.
authors.affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization, Kannondai, Tsukuba, Ibaraki, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Hirota
authors.forename: Shinya
authors.initials: S
authors.lastname: Shimizu
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shibahara
authors.forename: Takashi
authors.initials: T
authors.lastname: Isobe
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamada
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Tanimura
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D001715:Bird Diseases / Q000473:pathology; D015153:Blotting, Western; D001921:Brain / Q000473:pathology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005777:Geese; D007150:Immunohistochemistry / Q000379:methods*; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000276:immunology*; D014901:West Nile Fever / Q000473:pathology / Q000662:veterinary; D014902:West Nile virus / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014764:Viral Proteins