title: Ratiometric Ca²⁺ measurements using the FlexStation(®)Scanning Fluorometer.
abstract: The FlexStation(                   ®                 ) Scanning Fluorometer is a fluorescence plate reader that can measure intracellular Ca(2+) concentration using both single-wavelength and dual-wavelength fluorescent probes. The FlexStation uses a Xenon flashlamp and monochromators for both excitation and emission light to allow the use of a wide range of fluorescent indicators. The system incorporates a fluid transfer system for addition of test compounds from a source plate to the cell plate during data acquisition. Both plates are contained within a temperature-controlled unit that can be controlled accurately between room temperature and 45°C. The FlexStation can be configured to read a range of plate sizes. In this chapter generic methods for assessing intracellular Ca(2+) on the FlexStation using ratiometric dyes are described.
authors.affiliation: Neurology & GI Centre of Excellence for Drug Discovery, GlaxoSmithKline Research and Development Limited, Harlow, Essex, UK.
authors.forename: Ian C B
authors.initials: IC
authors.lastname: Marshall
authors.forename: Izzy
authors.initials: I
authors.lastname: Boyfield
authors.forename: Shaun
authors.initials: S
authors.lastname: McNulty
mesh_terms: D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D005456:Fluorescent Dyes / Q000378:metabolism; D005470:Fluorometry / Q000295:instrumentation* / Q000379:methods*; D016257:Fura-2 / Q000378:metabolism; D006801:Humans
chemical_list: D005456:Fluorescent Dyes; D002118:Calcium; D016257:Fura-2