title: 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone inhibits apoptosis of MIN6 cells via improving mitochondrial function.
abstract: Mitochondrial dysfunction due to oxidative stress and concomitant beta-cell apoptosis may play a key role in type 2 diabetes. Inhibiting beta-cell apoptosis through ameliorating oxidative mitochondrial dysfunction with specific natural products may have preventive or therapeutic potential. In this study, the anti-apoptotic effect of 2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC), a isolated chalcone from the buds of Cleistocalyx operculatus, on H2O2 induced MIN6 cells damage was investigated. Exposure to H2O2 at 250 microM for 3 h, the viability of MIN6 cells was significantly decreased and the apoptosis apparently occurred. A pre-treatment with DMC at the concentrations of 12.5-25 microM, before H2O2 addition, reduced nucleus fragmentation, decreased endogenous reactive oxygen species (ROS) production, and improved mitochondrial potential (MMP), and consequently, inhibited apoptosis. Furthermore, decreased activities of caspase-3 and caspase-9 were observed. These results clearly demonstrated DMC protected MIN6 cells against apoptosis due to its highly protective effect on mitochondria, and thus, it has great potential as a candidate drug for the diabetes care.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Yingdi
authors.initials: Y
authors.lastname: Luo
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Lu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001562:Benzimidazoles; D020169:Caspases / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D047188:Chalcones / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D008564:Membrane Potentials / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D051336:Mitochondrial Membranes / Q000187:drug effects; D016877:Oxidants / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: C501649:2',4'-dihydroxy-6'-methoxy-3',5'-dimethylchalcone; D001562:Benzimidazoles; D047188:Chalcones; D004396:Coloring Agents; D016877:Oxidants; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide; D020169:Caspases; C017807:bisbenzimide ethoxide trihydrochloride