title: [Mechanism of phenotype difference in biofilm formation by Candida albicans isolated from the respiratory tract].
abstract: OBJECTIVE
  To explore the molecular mechanism of the differences in biofilm formation abilities of Candida albicans isolated from the respiratory tract.
  
  
  METHODS
  The biofilms formed by Candida albicans isolates from the respiratory tract and the standard strain ATCC90028 were examined for bacterial proliferation using XTT reduction assay. The mRNA expression of CPH1, EFG1, ALS3 and HWP1 in the isolates were measured with fluorescent quantitative RT-PCR.
  
  
  RESULTS
  XTT reduction assay demonstrated a strong ability of biofilm formation in 8 clinical isolates, and a relatively low biofilm formation ability in 7 clinical isolates and ATCC90028 strain. The strong and weak biofilm formers showed significant differences in ALS3 and HWP1 mRNA expressions (P<0.05) but not in EFG1 or CPH1 mRNA expressions (P>0.05).
  
  
  CONCLUSIONS
  The clinical isolates from the respiratory tract have different biofilm formation abilities under regulation by genes other than the transcription factors CPH1 and EFG1.
authors.affiliation: Department of Respiratory Medicine, Second Affiliated Hospital, Guangzhou Medical College, Guangzhou 510260, China. yangjun9909@126.com
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Tiantuo
authors.initials: T
authors.lastname: Zhang
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Zhu
mesh_terms: D018441:Biofilms; D002176:Candida albicans / Q000145:classification / Q000235:genetics* / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005091:Exons; D005656:Fungal Proteins / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000378:metabolism; D010641:Phenotype; D012137:Respiratory System / Q000382:microbiology*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: C113620:ALS3 protein, Candida albicans; C090837:CPH1 protein, Candida albicans; D004268:DNA-Binding Proteins; C121438:EFG1 protein, Candida albicans; D005656:Fungal Proteins; C118102:HWP1 protein, Candida albicans; D008562:Membrane Glycoproteins; D014157:Transcription Factors