title: A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins.
abstract: Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. Here we show using a fully reconstituted T7 bacteriophage system that eukaryotic proteins are not required to tether the Polycomb complex PRC1 to templates during DNA replication. Instead, DNA binding by PRC1 can withstand passage of a simple replication fork.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
authors.forename: Bettina M
authors.initials: BM
authors.lastname: Lengsfeld
authors.forename: Kayla N
authors.initials: KN
authors.lastname: Berry
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Ghosh
authors.forename: Masateru
authors.initials: M
authors.lastname: Takahashi
authors.forename: Nicole J
authors.initials: NJ
authors.lastname: Francis
mesh_terms: D017123:Bacteriophage T7 / Q000235:genetics; D001667:Binding, Competitive; D004265:DNA Helicases / Q000737:chemistry; D004261:DNA Replication; D004279:DNA, Viral / Q000737:chemistry*; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry; D063146:Polycomb-Group Proteins / Q000737:chemistry*; D011485:Protein Binding; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D004279:DNA, Viral; D063146:Polycomb-Group Proteins; D014764:Viral Proteins; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases