title: In vitro and in vivo treatments of Echinococcus granulosus with Huaier aqueous extract and albendazole liposome.
abstract: The aim of this study was to investigate the in vitro and in vivo efficacies of chemotherapy employing albendazole liposome (L-ABZ), Huaier aqueous extract, and a Huaier aqueous extract/L-ABZ combination against Echinococcus granulosus. Protoscolices of E. granulosus were incubated in vitro with the two drugs, either separately or in combination, at the following final concentrations: 2 mg/mL Huaier aqueous extract, 10 μg/mL L-ABZ, and 2 mg/mL Huaier aqueous extract + 10 μg/mL L-ABZ. Huaier aqueous extract and L-ABZ displayed slower protoscolicidal activity when applied separately than when used in combination. The maximum protoscolicidal effect was found with the combination Huaier aqueous extract + L-ABZ. Despite the low Huaier aqueous extract + L-ABZ concentrations used, protoscolex viability dropped rapidly. In vivo studies were performed on mice injected with protoscolices of E. granulosus. Huaier aqueous extract and L-ABZ were administered three times a week for a period of 4 months by the oral route. Huaier aqueous extract in E. granulosus-infected mice was effective. Combined application of both drugs did increase the treatment efficacy. In conclusion, the outcomes obtained clearly demonstrated that in vitro and in vivo treatment with Huaier aqueous extract and L-ABZ is effective against E. granulosus.
authors.affiliation: The Hepatic Surgery Center, Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Lv
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Jiang
authors.forename: Min
authors.initials: M
authors.lastname: Liao
authors.forename: Hongli
authors.initials: H
authors.lastname: Sun
authors.forename: Shijie
authors.initials: S
authors.lastname: Zhang
authors.forename: Xinyu
authors.initials: X
authors.lastname: Peng
mesh_terms: D015766:Albendazole / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000871:Anthelmintics / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage; D004359:Drug Therapy, Combination; D004443:Echinococcosis / Q000188:drug therapy* / Q000469:parasitology; D048209:Echinococcus granulosus / Q000187:drug effects*; D005260:Female; D008081:Liposomes / Q000008:administration & dosage; D051379:Mice; D016019:Survival Analysis; D055454:Trametes / Q000737:chemistry*; D016896:Treatment Outcome
chemical_list: D000871:Anthelmintics; D004337:Drug Carriers; D008081:Liposomes; D015766:Albendazole