title: Preliminary studies on the development of IgA-loaded chitosan-dextran sulphate nanoparticles as a potential nasal delivery system for protein antigens.
abstract: This study describes the development of a biodegradable nanoparticulate system for the intranasal delivery of multiple proteins. Chitosan (CS)-dextran sulphate (DS) nanoparticles were developed and optimised for the incorporation of pertussis toxin (PTX) and a potential targeting ligand (immunoglobulin-A, IgA). In vitro characterization and in vivo uptake studies were performed for the evaluation of developed nanoparticles. The ratio of CS to DS, the order of mixing and pH of nanoparticle suspension were identified as important formulation factors governing the size and zeta potential of nanoparticles. An optimised CS-DS nanoparticle formulation prepared with the CS to DS weight ratio of 3 : 1 was used to load PTX and/or IgA. Entrapment efficiency of >90% was obtained for both. The in vivo uptake of IgA-loaded CS-DS nanoparticles in mice showed a preferential uptake of nanoparticles probably by nasal membranous or microfold cells following intranasal administration. The results of this study indicate the potential application of IgA-loaded CS-DS nanoparticles as a nasal vaccine delivery system.
authors.affiliation: School of Pharmacy, CHIRI, Western Australia Biomedical Research Institute, Curtin University, GPO Box U1987, Perth, Western Australia 6845, Australia.
authors.forename: Sameer
authors.initials: S
authors.lastname: Sharma
authors.forename: Heather A E
authors.initials: HA
authors.lastname: Benson
authors.forename: Trilochan K S
authors.initials: TK
authors.lastname: Mukkur
authors.forename: Paul
authors.initials: P
authors.lastname: Rigby
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D016264:Dextran Sulfate / Q000008:administration & dosage*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006863:Hydrogen-Ion Concentration; D007070:Immunoglobulin A / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles
chemical_list: D000941:Antigens; D007070:Immunoglobulin A; D016264:Dextran Sulfate