title: Human parathyroid hormone increases the mRNA expression of the IGF system and hematopoietic growth factors in osteoblasts, but does not influence expression in mesenchymal stem cells.
abstract: Osteoblasts, which are derived from pluripotent mesenchymal stem cells (MSCs), play an important role in hematopoiesis. Human parathyroid hormone (hPTH) induces osteoblasts to produce many factors that are essential to hematopoietic stem cells. However, little is known about the impact of hPTH on MSCs to enhance hematopoiesis. We determined the optimal dose of hPTH that was necessary in vitro for increased osteoblast function. In addition, we compared MSC and osteoblast function to explore the role of hPTH in hematopoiesis. The mRNA expression levels of granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin 6, stromal cell-derived factor 1, insulin-like growth factor 1 (IGF-1), IGF-2, insulin-like growth factor-binding protein 1 (IGFBP-1), IGFBP-2, and IGFBP-3 were comparable in osteoblasts and human cord blood-derived MSCs. However, G-CSF, GM-CSF, IGF-2, IGFBP-1, IGFBP-2, and IGFBP-3 expression levels in osteoblasts were markedly increased after treatment with 50 or 100 nM of hPTH. In conclusion, hPTH does not affect the ability of MSCs to differentiate into osteoblasts. In addition, hPTH may enhance hematopoiesis by activating the IGF system (IGF-2, IGFBP-1, IGFBP-2, and IGFBP-3) and hematopoietic growth factors (G-CSF and GM-CSF) in osteoblasts, but not in MSCs.
authors.affiliation: Department of Pediatrics, Wonkwang University Sanbon Medical Center, Gunpo, Korea.
authors.forename: Jong-Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Kyou-Jung
authors.initials: KJ
authors.lastname: Hwang
authors.forename: Mi-Yeon
authors.initials: MY
authors.lastname: Kim
authors.forename: Yeon-Jung
authors.initials: YJ
authors.lastname: Lim
authors.forename: In-Joon
authors.initials: IJ
authors.lastname: Seol
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Jin
authors.forename: Yun-Kyung
authors.initials: YK
authors.lastname: Jang
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Choi
authors.forename: Wonil
authors.initials: W
authors.lastname: Oh
authors.forename: Youl-Hee
authors.initials: YH
authors.lastname: Cho
authors.forename: Young-Ho
authors.initials: YH
authors.lastname: Lee
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010281:Parathyroid Hormone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis*; D013002:Somatomedins / Q000235:genetics*
chemical_list: C470125:PTH protein, human; D010281:Parathyroid Hormone; D012333:RNA, Messenger; D013002:Somatomedins; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D000469:Alkaline Phosphatase