title: Catuaba (Trichilia catigua) prevents against oxidative damage induced by in vitro ischemia-reperfusion in rat hippocampal slices.
abstract: Oxidative stress is implicated in brain damage associated with ischemia-reperfusion. Natural antioxidants found in some plants used in folk medicine have been indicated as potential neuroprotective agents. Here we investigated whether Trichilia catigua, a traditional Brazilian herbal medicine alleged to exhibit a variety of neuropharmacological properties (antidepressant, anti-neurasthenic, anti-inflammatory etc.), could have neuroprotective properties in rat hippocampal slices subjected to 2 h oxygen and glucose deprivation (OGD) followed by 1 h reperfusion. Ischemia-reperfusion (I/R) significantly decreased mitochondrial viability, increased dichlorofluorescein oxidation above control both in the incubation medium and slices homogenates, increased lactate dehydrogenase into the incubation medium and decreased non-protein thiols. T. catigua (40-100 μg/mL) protected slices from the deleterious effects of OGD when present before OGD and during the reperfusion periods. Oxidative stress in the medium was also determined under different conditions and the results demonstrated that T. catigua could not protect slices from I/R when it was added to the medium after ischemic insult. Although the translation to a real in vivo situation of I/R is difficult to be done, the results indicated that T. catigua should be used as preventive and not as a curative agent against brain damage.
authors.affiliation: Departamento de Química, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS CEP 97105-900, Brazil.
authors.forename: Jean Paul
authors.initials: JP
authors.lastname: Kamdem
authors.forename: Emily Pansera
authors.initials: EP
authors.lastname: Waczuk
authors.forename: Ige Joseph
authors.initials: IJ
authors.lastname: Kade
authors.forename: Caroline
authors.initials: C
authors.lastname: Wagner
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
authors.forename: Diogo Onofre
authors.initials: DO
authors.lastname: Souza
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: Rocha
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D029582:Meliaceae / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications* / Q000378:metabolism
chemical_list: D010936:Plant Extracts