title: Studies related to Norway spruce galactoglucomannans: chemical synthesis, conformation analysis, NMR spectroscopic characterization, and molecular recognition of model compounds.
abstract: Galactoglucomannan (GGM) is a polysaccharide mainly consisting of mannose, glucose, and galactose. GGM is the most abundant hemicellulose in the Norway spruce (Picea abies), but is also found in the cell wall of flax seeds, tobacco plants, and kiwifruit. Although several applications for GGM polysaccharides have been developed in pulp and paper manufacturing and the food and medical industries, attempts to synthesize and study distinct fragments of this polysaccharide have not been reported previously. Herein, the synthesis of one of the core trisaccharide units of GGM together with a less-abundant tetrasaccharide fragment is described. In addition, detailed NMR spectroscopic characterization of the model compounds, comparison of the spectral data with natural GGM, investigation of the acetyl-group migration phenomena that takes place in the polysaccharide by using small model compounds, and a binding study between the tetrasaccharide model fragment and a galactose-binding protein (the toxin viscumin) are reported.
authors.affiliation: Laboratory of Organic Chemistry, Åbo Akademi University, Piispankatu 8, 20500 Åbo, Finland.
authors.forename: Filip S
authors.initials: FS
authors.lastname: Ekholm
authors.forename: Ana
authors.initials: A
authors.lastname: Ardá
authors.forename: Patrik
authors.initials: P
authors.lastname: Eklund
authors.forename: Sabine
authors.initials: S
authors.lastname: André
authors.forename: Hans-Joachim
authors.initials: HJ
authors.lastname: Gabius
authors.forename: Jesús
authors.initials: J
authors.lastname: Jiménez-Barbero
authors.forename: Reko
authors.initials: R
authors.lastname: Leino
mesh_terms: D000373:Agglutinins / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D008968:Molecular Conformation; D009664:Norway; D028222:Picea / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D000373:Agglutinins; D008351:Mannans; C443804:galactoglucomannan