title: Coilin levels modulate cell cycle progression and γH2AX levels in etoposide treated U2OS cells.
abstract: Coilin is considered the Cajal body (CB) marker protein. In this report, we investigated the role of coilin in the DNA damage response and found that coilin reduction correlated with significantly increased levels of soluble γH2AX in etoposide treated U2OS cells. Additionally, coilin levels influenced the proliferation rate and cell cycle distribution of cells exposed to etoposide. Moreover, coilin overexpression inhibited nucleolar localization of endogenous coilin in etoposide treated U2OS cells. Collectively, these data provide additional evidence for coilin and CBs in the DNA damage response.
authors.affiliation: Department of Biochemistry, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA.
authors.forename: Venkatramreddy
authors.initials: V
authors.lastname: Velma
authors.forename: Zunamys I
authors.initials: ZI
authors.lastname: Carrero
authors.forename: Carmen B
authors.initials: CB
authors.lastname: Allen
authors.forename: Michael D
authors.initials: MD
authors.lastname: Hebert
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000502:physiology*; D045744:Cell Line, Tumor; D002466:Cell Nucleolus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D020541:Coiled Bodies / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D005047:Etoposide / Q000494:pharmacology*; D055785:Gene Knockdown Techniques; D006657:Histones / Q000378:metabolism*; D006801:Humans; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D012995:Solubility
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C468783:H2AX protein, human; D006657:Histones; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; C068921:p80-coilin; D005047:Etoposide