title: Identification of peptides from foot-and-mouth disease virus structural proteins bound by class I swine leukocyte antigen (SLA) alleles, SLA-1*0401 and SLA-2*0401.
abstract: Characterization of the peptide-binding specificity of swine leukocyte antigen (SLA) class I and II molecules is critical to the understanding of adaptive immune responses of swine toward infectious pathogens. Here, we describe the complete binding motif of the SLA-2*0401 molecule based on a positional scanning combinatorial peptide library approach. By combining this binding motif with data achieved by applying the NetMHCpan peptide prediction algorithm to both SLA-1*0401 and SLA-2*0401, we identified high-affinity binding peptides. A total of 727 different 9mer and 726 different 10mer peptides within the structural proteins of foot-and-mouth disease virus (FMDV), strain A24 were analyzed as candidate T-cell epitopes. Peptides predicted by the NetMHCpan were tested in ELISA for binding to the SLA-1*0401 and SLA-2*0401 major histocompatibility complex class I proteins. Four of the 10 predicted FMDV peptides bound to SLA-2*0401, whereas five of the nine predicted FMDV peptides bound to SLA-1*0401. These methods provide the characterization of T-cell epitopes in response to pathogens in more detail. The development of such approaches to analyze vaccine performance will contribute to a more accelerated improvement of livestock vaccines by virtue of identifying and focusing analysis on bona fide T-cell epitopes.
authors.affiliation: Foreign Animal Disease Unit, Plum Island Animal Disease Center, Agricultural Research Service, USDA, Greenport, NY 11944, USA.
authors.forename: L E
authors.initials: LE
authors.lastname: Pedersen
authors.forename: M
authors.initials: M
authors.lastname: Harndahl
authors.forename: M
authors.initials: M
authors.lastname: Nielsen
authors.forename: J R
authors.initials: JR
authors.lastname: Patch
authors.forename: G
authors.initials: G
authors.lastname: Jungersen
authors.forename: S
authors.initials: S
authors.lastname: Buus
authors.forename: W T
authors.initials: WT
authors.lastname: Golde
mesh_terms: D000483:Alleles; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D018984:Epitopes, T-Lymphocyte / Q000235:genetics; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics*; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006801:Humans; D019151:Peptide Library; D011485:Protein Binding; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013552:Swine; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*; D001613:beta 2-Microglobulin / Q000378:metabolism
chemical_list: D018984:Epitopes, T-Lymphocyte; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D019151:Peptide Library; D011994:Recombinant Proteins; D015678:Viral Structural Proteins; D001613:beta 2-Microglobulin; C438668:swine leukocyte antigen