title: The effect of temperature on L-lactic acid production and metabolite distribution of Lactobacillus casei.
abstract: The effect of temperature on the growth and L-lactic acid production of Lactobacillus casei G-03 was investigated in a 7-L bioreactor. It was found that the maximum specific growth rate (0.27 hr⁻¹) and L-lactic acid concentration (160.2 g L⁻¹) were obtained at a temperature of 41°C. Meanwhile, the maximum L-lactic acid yield, productivity, and dry cell weight were up to 94.1%, 4.44 g L⁻¹ hr⁻¹, and 4.30 g L⁻¹, respectively. At lower or higher temperature, the Lactobacillus casei G-03 showed lower acid production and biomass. Moreover, the main metabolite distribution of strain G-03 response to variations in temperatures was studied. The results suggested that temperature has a remarkable effect on metabolite distribution, and the maximum carbon flux toward lactic acid at the pyruvate node was obtained at 41°C, which had the minimum carbon flux toward acetic acid.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, JiangSu Province, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Qin
authors.forename: Sai-Sai
authors.initials: SS
authors.lastname: Gong
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Ge
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Zhang
mesh_terms: D019342:Acetic Acid; D019149:Bioreactors; D003470:Culture Media / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000096:biosynthesis*; D007780:Lacticaseibacillus casei / Q000254:growth & development / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism; D013696:Temperature
chemical_list: D003470:Culture Media; D019344:Lactic Acid; D019289:Pyruvic Acid; D005947:Glucose; D019342:Acetic Acid