title: [Regulation effect of scorpio and scolopendra on CD4 + CD25 + FoxP3 + Treg cell in peripheral blood from rats with collagen-induced arthritis (CIA)].
abstract: OBJECTIVE
  To study the regulation effect of Scorpio and Scolopendra on CD4 + CD25 + FoxP3 + Treg Cell in peripheral blood from rats with collagen-induced arthritis.
  
  
  METHODS
  Sixty female Wistar rats were randomly divided into 6 groups, normal control group, model control group, low-dose, middle-dose and high-dose Scorpio and Scolopendra group,and the type II collagen group. Rats' arthritis was induced by collagen. The number of CD4 + CD25 + FoxP3 + Treg cell in peripheral blood was tested by flow cytometry, and the level of IL-2 in serum was detected by enzyme linked immunosorbent assay.
  
  
  RESULTS
  Compared with the model groups,the levels of CD4 + CD25 + Treg cell and CD4 + CD25 + FoxP3 + Treg cell were increased obviously in the high and low dose of Scorpio and Scolopendra groups (P < 0.05 or P < 0.01), while the level of IL-2 in serum was decreased remarkably in the middle and low dose of Scorpio and Scolopendra groups (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  It is realized that Scorpio and Scolopendra effectively treat RA by regulating the level of CD4 + CD25 + FoxP3 + Treg cell to restore immunological tolerance.
authors.affiliation: Technology College, Jiangxi University of Traditional Chinese Medicine Science, Nanchang 330025, China.
authors.forename: Duan-Yong
authors.initials: DY
authors.lastname: Liu
authors.forename: Hai-Mei
authors.initials: HM
authors.lastname: Zhao
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Huang
authors.forename: Zhi-Qin
authors.initials: ZQ
authors.lastname: Zuo
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Shao-Min
authors.initials: SM
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000097:blood / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology; D018791:CD4 Lymphocyte Count; D024043:Collagen Type II / Q000276:immunology; D000077743:Diterpene Alkaloids; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D051858:Forkhead Transcription Factors / Q000276:immunology; D007376:Interleukin-2 / Q000097:blood / Q000276:immunology; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*
chemical_list: D000893:Anti-Inflammatory Agents; D024043:Collagen Type II; D000077743:Diterpene Alkaloids; D004365:Drugs, Chinese Herbal; D051858:Forkhead Transcription Factors; C500522:Foxp3 protein, rat; D007376:Interleukin-2; D053645:Interleukin-2 Receptor alpha Subunit; C574918:scorpio; C574917:scolopendra alkaloids