title: Isolation and characterization of 13 novel polymorphic microsatellite markers for Pinus koraiensis (Pinaceae).
abstract: OBJECTIVE
  Thirteen polymorphic microsatellite markers were developed to investigate the genetic diversity and population structure of Pinus koraiensis. •
  
  
  RESULTS
  Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method with three specific PCR primers for screening the positive clones, 13 loci were found to be polymorphic in 78 individuals of P. koraiensis. Across all of the P. koraiensis samples, the number of alleles per locus ranged from two to 11. •
  
  
  CONCLUSIONS
  These polymorphic markers will be useful for conservation genetics studies of this species and to inform the development of effective P. koraiensis conservation programs.
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, People's Republic of China.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Yu
authors.forename: Chang-Mei
authors.initials: CM
authors.lastname: Chen
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Tang
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Yuan
authors.forename: Chun-Jing
authors.initials: CJ
authors.lastname: Wang
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
mesh_terms: D017931:DNA Primers / Q000378:metabolism; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D028223:Pinus / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D017931:DNA Primers; D018744:DNA, Plant