title: Cu(GlyGlyHis) effects on MCF7 cells: copper uptake, reactive oxygen species generation and membrane topography changes.
abstract: Elevated levels of copper have been detected in various types of human cancer cells, such as breast cancer cells, and a number of mechanisms have been proposed to explain the action and influence of copper on tumor progress. In this work, we found that stimulating the proliferation of mammary epithelial MCF7 cells with the high-redox-potential copper complex Cu (GlyGlyHis) is associated with the copper-induced intracellular generation of reactive oxygen species (ROS) that induces lipid peroxidation and causes increased roughness of external cell membranes, which leads to the formation of larger cell domes. The results presented herein provide new insights into the molecular link between copper and the proliferation of breast cancer cells and, consequently, into the mechanism by which changes in redox balance and ROS accumulation regulates cell membrane roughness.
authors.affiliation: Center for Natural Sciences and Humanities, Federal University of ABC - UFABC, Avenida dos Estados, 5001, Bloco B, 09210-170, Santo André, SP, Brazil.
authors.forename: Andreza C
authors.initials: AC
authors.lastname: Matias
authors.forename: Nathália
authors.initials: N
authors.lastname: Villa dos Santos
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Chelegão
authors.forename: Cassiana S
authors.initials: CS
authors.lastname: Nomura
authors.forename: Pablo A
authors.initials: PA
authors.lastname: Fiorito
authors.forename: Giselle
authors.initials: G
authors.lastname: Cerchiaro
mesh_terms: D015153:Blotting, Western; D003300:Copper / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D061986:MCF-7 Cells; D018625:Microscopy, Atomic Force; D009842:Oligopeptides / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013054:Spectrophotometry, Atomic
chemical_list: D009842:Oligopeptides; D017382:Reactive Oxygen Species; C037026:diglycyl-histidine; D003300:Copper