title: Field evaluation of repellency of a polyherbal essential oil against blackflies and its dermal toxicity using rat model.
abstract: In the present study we have evaluated the repellent activity of mixture of Curcuma longa, Zanthoxylum limonella and Pogostemon heyneanus essential oils in 1:1:2 ratio at 5%, 10% and 20% concentration against blackflies in northeastern India. Initially the essential oil mixture tested here has been found effective against Aedes albopictus mosquitoes. The average protection recorded in 20% concentration (170.56 ± 4.0; 95% CI = 162.09-179.02) was higher as compared to other two concentrations (F = 90.2; p<0.0001; df = 53). Percentage repellency and repellency index was found to be higher in 20% concentration (p ≤ 0.017). No appreciable clinical and behavioral signs were observed in the acute dermal toxicity using rat model. No changes were observed in biochemical profiles of treatment group animals. Similarly, no prominent lesions were observed in vital organs of treatment in both the sexes. The study concludes that tested repellent is safe for use and has multi-insects repellent property.
authors.affiliation: Defence Research Laboratory Tezpur, Assam 784001. sunildhiman81@gmail.com
authors.forename: D
authors.initials: D
authors.lastname: Sunil
authors.forename: R
authors.initials: R
authors.lastname: Bipul
authors.forename: C
authors.initials: C
authors.lastname: Pronobesh
authors.forename: N G
authors.initials: NG
authors.lastname: Das
authors.forename: S
authors.initials: S
authors.lastname: Hazarika
authors.forename: R K
authors.initials: RK
authors.lastname: Bhola
authors.forename: V
authors.initials: V
authors.lastname: Vijay
authors.forename: S
authors.initials: S
authors.lastname: Lokendra
mesh_terms: D000330:Aedes / Q000187:drug effects; D000818:Animals; D030024:Curcuma / Q000737:chemistry*; D005260:Female; D007194:India; D007302:Insect Repellents / Q000494:pharmacology*; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D009822:Oils, Volatile / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012843:Simuliidae / Q000187:drug effects*; D023382:Toxicity Tests, Acute; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D007302:Insect Repellents; D009822:Oils, Volatile; D010938:Plant Oils