title: Sphingobium fontiphilum sp. nov., isolated from a freshwater spring.
abstract: To investigate the biodiversity of bacteria in the spring water of the Chengcing Lake Park in Taiwan, a Gram-stain-negative, rod-shaped, non-motile, non-spore-forming and aerobic bacterial strain, designated strain Chen16-4(T), was isolated and characterized in a taxonomic study using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the closest relatives of strain Chen16-4(T) were Sphingobium amiense YT(T), Sphingobium yanoikuyae GIFU 9882(T) and Sphingobium scionense WP01(T), with sequence similarities of 97.6, 97.1 and 97.0 %, respectively. A phylogenetic tree obtained with 16S rRNA gene sequences indicated that strain Chen16-4(T) and these three closest relatives formed an independent phylogenetic clade within the genus Sphingobium. The polar lipid pattern, the presence of spermidine and ubiquinone Q-10, the predominance of C18 : 1ω7c in the cellular fatty acid profile and the DNA G+C content also supported affiliation of the isolate to the genus Sphingobium. The DNA-DNA relatedness of strain Chen16-4(T) with respect to recognized species of the genus Sphingobium was less than 70 %. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain Chen16-4(T) represents a novel species in the genus Sphingobium, for which the name Sphingobium fontiphilum sp. nov. is proposed. The type strain is Chen16-4(T) ( = BCRC 80308(T) = LMG 26342(T) = KCTC 23559(T)).
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Shih-Yi
authors.initials: SY
authors.lastname: Sheu
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Yu-Wen
authors.initials: YW
authors.lastname: Shiau
authors.affiliation: Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Yan-Ting
authors.initials: YT
authors.lastname: Wei
authors.affiliation: Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
authors.forename: Wen-Ming
authors.initials: WM
authors.lastname: Chen
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000032:analysis; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013624:Taiwan; D014451:Ubiquinone / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine