title: Sealing ability of mineral trioxide aggregate (MTA) combined with distilled water, chlorhexidine, and doxycycline.
abstract: The aim of this study was to evaluate the sealing ability of mineral trioxide aggregate (MTA Bio) combined with different mixing agents (distilled water, chlorhexidine, doxycycline), used as an apical root-end filling material. Forty-two extracted human teeth were divided into three groups (n = 12); six teeth were used as controls. Root-ends were resected at 90 degrees, 3 mm from the apex. Root-end cavities were prepared using ultrasonic tips and filled with MTA Bio plus distilled water, 2% chlorhexidine solution, or 10% doxycycline solution. Apical sealing was assessed by microleakage of 50% silver nitrate solution. Roots were longitudinally sectioned in a buccolingual plane and analyzed using an operating microscope (20× magnification). Depth of dye leakage into the dentinal walls was measured in millimeters. Results were analyzed using ANOVA and Tukey's test (P = 0.05). MTA Bio plus distilled water showed significantly higher mean leakage results (1.06 mm) when compared with MTA Bio plus doxycycline (0.61 mm), and higher, although not significant, results when compared with MTA Bio plus chlorhexidine (0.79 mm). In conclusion, replacing distilled water with two biologically active mixing agents (doxycycline and chlorhexidine) did not alter the sealing properties of MTABio. The antimicrobial properties of these combinations should be further investigated.
authors.affiliation: Department of Endodontics, São Leopoldo Mandic School of Dentistry and Dental Research Center, Campinas, SP, Brazil. roberta.aranha@terra.com.br
authors.forename: Roberta A A
authors.initials: RA
authors.lastname: Arruda
authors.forename: Rodrigo S
authors.initials: RS
authors.lastname: Cunha
authors.forename: Kenner B
authors.initials: KB
authors.lastname: Miguita
authors.forename: Cláudia F M
authors.initials: CF
authors.lastname: Silveira
authors.forename: Alexandre S
authors.initials: AS
authors.lastname: De Martin
authors.forename: Sérgio L
authors.initials: SL
authors.lastname: Pinheiro
authors.forename: Daniel G P
authors.initials: DG
authors.lastname: Rocha
authors.forename: Carlos E S
authors.initials: CE
authors.lastname: Bueno
mesh_terms: D017607:Aluminum Compounds / Q000737:chemistry*; D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local; D017610:Calcium Compounds / Q000737:chemistry*; D002710:Chlorhexidine; D004396:Coloring Agents; D003763:Dental Leakage / Q000517:prevention & control*; D004318:Doxycycline; D004338:Drug Combinations; D006801:Humans; D010087:Oxides / Q000737:chemistry*; D012184:Retrograde Obturation / Q000379:methods*; D012387:Root Canal Filling Materials / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry*; D012835:Silver Nitrate; D018709:Statistics, Nonparametric; D014867:Water
chemical_list: D017607:Aluminum Compounds; D000891:Anti-Infective Agents, Local; D017610:Calcium Compounds; D004396:Coloring Agents; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D014867:Water; D012835:Silver Nitrate; D004318:Doxycycline; D002710:Chlorhexidine