title: Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases.
abstract: Enzymatic recognition of SeLNA nucleotides was investigated. KOD XL DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of SeLNA-modified DNA templates was also efficiently achieved by Phusion and KOD XL DNA polymerases.
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane 4072, Australia.
authors.forename: Megan
authors.initials: M
authors.lastname: Wheeler
authors.forename: Antoine
authors.initials: A
authors.lastname: Chardon
authors.forename: Astrid
authors.initials: A
authors.lastname: Goubet
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Morihiro
authors.forename: Sze Yee
authors.initials: SY
authors.lastname: Tsan
authors.forename: Stacey L
authors.initials: SL
authors.lastname: Edwards
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kodama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Obika
authors.forename: Rakesh N
authors.initials: RN
authors.lastname: Veedu
mesh_terms: D001483:Base Sequence; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D016133:Polymerase Chain Reaction; D012643:Selenium / Q000737:chemistry*
chemical_list: D009841:Oligonucleotides; C477371:locked nucleic acid; D004259:DNA-Directed DNA Polymerase; D012643:Selenium