title: Inhibition of glioma cell lysosome exocytosis inhibits glioma invasion.
abstract: Cancer cells invade by secreting enzymes that degrade the extracellular matrix and these are sequestered in lysosomal vesicles. In this study, the effects of the selective lysosome lysing drug GPN and the lysosome exocytosis inhibitor vacuolin-1 on lysosome exocytosis were studied to determine their effect on glioma cell migration and invasion. Both GPN and vacuolin-1 evidently inhibited migration and invasion in transwell experiments and scratch experiments. There are more lysosomes located on the cell membrane of glioma cells than of astrocytes. GPN decreased the lysosome number on the cell membrane. We found that rab27A was expressed in glioma cells, and colocalized with cathepsin D in lysosome. RNAi-Rab27A inhibited lysosome cathepsin D exocytosis and glioma cell invasion in an in vitro assay. Inhibition of cathepsin D inhibited glioma cell migration. The data suggest that the inhibition of lysosome exocytosis from glioma cells plays an important modulatory role in their migration and invasion.
authors.affiliation: Department of Pathology, Department of Neurobiology, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Yijiang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Keqing
authors.initials: K
authors.lastname: Zhu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001253:Astrocytes / Q000378:metabolism; D002402:Cathepsin D / Q000037:antagonists & inhibitors / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D004151:Dipeptides / Q000494:pharmacology*; D005089:Exocytosis / Q000187:drug effects*; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D006801:Humans; D051907:Lysosomal Membrane Proteins / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects* / Q000502:physiology; D009361:Neoplasm Invasiveness; D010436:Pepstatins / Q000494:pharmacology; D021381:Protein Transport; D034622:RNA Interference; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D000075745:rab27 GTP-Binding Proteins; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D000970:Antineoplastic Agents; D002402:Cathepsin D; D004151:Dipeptides; D006576:Heterocyclic Compounds, 4 or More Rings; D051907:Lysosomal Membrane Proteins; D010436:Pepstatins; D020691:rab GTP-Binding Proteins; D000075745:rab27 GTP-Binding Proteins; C489116:LAMP1 protein, human; D052118:Lysosomal-Associated Membrane Protein 1; C503870:vacuolin-1; C041865:glycylphenylalanine 2-naphthylamide; C066694:RAB27A protein, human; C031375:pepstatin