title: [Multi-channel analysis and comparison on absorption difference between paeoniflorin and Paeonia lactiflora extracts].
abstract: OBJECTIVE
  To compare the difference of the absorption difference between paeoniflorin monomer and Paeonia lactiflora extracts in rat intestinal canals by multi-channels.
  
  
  METHODS
  The rat intestinal perfusion model was established. The intestinal perfusate, bile and plasma samples were collected, in combination of the intestinal enzymes incubation test and the partition coefficient determination, to conduct a multi-channel analysis and comparison on absorption difference between paeoniflorin and P. lactiflora extracts.
  
  
  RESULTS
  In the same concentration, permeability coefficient P(eff)* of paeoniflorin in the different intestinal segments of P. lactiflora extract higher than the monomer of paeoniflorin, especially in the jejunum and ileum intestinal segments (P < 0. 05). However, both paeoniflorin monomer and P. lactiflora extracts showed less P(eff)* in four intestinal segments, with the former ranging between 0. 209-0.290 and the latter 0.252-0.333. No paeoniflorin and its metabolin was determined in bile samples, plasma samples and intestinal enzymes incubation samples of paeoniflorin monomer and P. lactiflora extracts.
  
  
  CONCLUSIONS
  Compared with the paeoniflorin monomer, P. lactiflora extract showed significantly increase in P(eff)*, which indicated that other ingredients in the extract can improve the absorption of paeoniflorin. However, due to the poor absorption of paeoniflorin, this effect fails to increase the concentration of paeoniflorin in bile and plasma within short period of time.
authors.affiliation: Key Laboratory of Chinese Medicine New Drug Delivery Systems, Jiangsu Institute of Traditional Chinese Medicine, Key Laboratory of Chinese Medicine Oral Formulations Drug Delivery Systems, State Administration of Traditional Chinese Medicine, Nanjing 210028, China. ychen202@yahoo.com.cn
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Ran
authors.initials: R
authors.lastname: Xin
authors.forename: Jinyan
authors.initials: J
authors.lastname: Wang
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Jia
mesh_terms: D000818:Animals; D001565:Benzoates / Q000493:pharmacokinetics*; D001952:Bridged-Ring Compounds / Q000493:pharmacokinetics*; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005960:Glucosides / Q000493:pharmacokinetics*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D039821:Monoterpenes; D029596:Paeonia / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides