title: Moderate traumatic brain injury promotes neural precursor proliferation without increasing neurogenesis in the adult hippocampus.
abstract: Traumatic brain injury (TBI) promotes neural stem/progenitor cell (NSC) proliferation in the adult hippocampus; however, it remains inconclusive whether proliferation of these cells results in newly generated mature neurons, leading to increased neurogenesis. When we traced the fates of proliferating cells labeled with bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) we found that the number of BrdU-positive cells increased in the hippocampus of TBI mice compared to the sham control. However, double immunostaining to distinguish their cell types showed that most of these cells were glia, and that only a small subpopulation is newborn granular neurons. There was no significant difference with respect to neurogenesis in the adult hippocampus between the injured and the control mice. These results indicate that TBI promotes cell proliferation including astrocyte activation and NSC proliferation. Nevertheless, the majority of the BrdU-positive cells are glia. The neurogenesis is not increased by TBI. These data suggest that TBI activates through promotion of NSC proliferation an innate repair and/or plasticity mechanism in the brain. However, additional intervention is required to increase neurogenesis for successfully repairing the damaged brain following TBI.
authors.affiliation: Spinal Cord and Brain Injury Research Group, Stark Neuroscience Research Institute, and Department of Neurological Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
authors.forename: Xiang
authors.initials: X
authors.lastname: Gao
authors.forename: Jinhui
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D000963:Antimetabolites; D001930:Brain Injuries / Q000473:pathology*; D001973:Bromodeoxyuridine; D002452:Cell Count; D049109:Cell Proliferation; D018891:Dentate Gyrus / Q000166:cytology / Q000473:pathology; D006624:Hippocampus / Q000166:cytology / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000502:physiology*; D055495:Neurogenesis / Q000502:physiology*; D009457:Neuroglia / Q000502:physiology
chemical_list: D000963:Antimetabolites; D001973:Bromodeoxyuridine