title: Overexpression of glycosylated proteins in cervical cancer recognized by the Machaerocereus eruca agglutinin.
abstract: In cervical cancer, glycosylation has been suggested as being involved in both its carcinogenesis and invasive capacity. In this work, we analyzed mucin type O-glycosylation in biopsies of invasive cervical cancer in FIGO stage II B through histochemistry using lectins specific for O-glycosidically linked glycans. Our results reveal that the lectin Machaerocereus eruca (MeA, specific for Gal in a Fucα1,2 (GalNAcα1,3) Galβ1,4) showed increased recognition of tumoral cells and tumoral stroma tissue compared to other lectins with similar specificity; healthy cervical tissue was negative for MeA. Trypsin treatment of recognized tissues abolished MeA's recognition;moreover, interaction of MeA was inhibited with oligosaccharides from mucin. As demonstrated by Western blot of 2-D electrophoresis, MeA recognized ten glycoproteins in the range from 122 to 42 kDa in cervical cancer lysates. The LC-ESI-MS/MS analysis of the MeAs' recognized peptides revealed that the latter matched mainly with the amino acid sequences of lamin A/C, vimentin, elongation factor 2, keratin 1, and beta actin. Our results suggest that MeA recognizes a complex of over-expressed O-glycosidically-linked proteins that play a relevant role in cervical cancer's invasive capacity. O-glycosylation participates in the disassembly of intercellular junctions favoring cancer progression.
authors.affiliation: Laboratorio de Inmunologia, Departamento de Bioquimica, Facultad de Medicina, UNAM, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Solórzano
authors.forename: Miguel
authors.initials: M
authors.lastname: Angel Mayoral
authors.forename: María
authors.initials: M
authors.lastname: de los Angeles Carlos
authors.forename: Jaime
authors.initials: J
authors.lastname: Berumen
authors.forename: Jorge
authors.initials: J
authors.lastname: Guevara
authors.forename: Francisco
authors.initials: F
authors.lastname: Raúl Chávez
authors.forename: Guillermo
authors.initials: G
authors.lastname: Mendoza-Hernández
authors.forename: Concepción
authors.initials: C
authors.lastname: Agundis
authors.forename: Edgar
authors.initials: E
authors.lastname: Zenteno
mesh_terms: D000373:Agglutinins / Q000378:metabolism*; D000818:Animals; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D006651:Histocytochemistry; D006801:Humans; D015151:Immunoblotting; D013058:Mass Spectrometry; D037121:Plant Lectins / Q000378:metabolism*; D034421:Sus scrofa; D014357:Trypsin / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000473:pathology*
chemical_list: D000373:Agglutinins; D006023:Glycoproteins; C057322:Machaerocereus eruca lectin; D037121:Plant Lectins; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate