title: Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry.
abstract: Toward new biotechnology by genetic alphabet expansion, we developed an efficient site-specific labeling method for large RNA molecules. The combination of unnatural base pair transcription and post-transcriptional modification by click chemistry enables simple RNA labeling with a wide variety of functional groups at desired positions, in a one-pot reaction.
authors.affiliation: RIKEN Systems and Structural Biology Center (SSBC), Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Ishizuka
authors.forename: Michiko
authors.initials: M
authors.lastname: Kimoto
authors.forename: Akira
authors.initials: A
authors.lastname: Sato
authors.forename: Ichiro
authors.initials: I
authors.lastname: Hirao
mesh_terms: D020029:Base Pairing; D057930:Click Chemistry; D007093:Imidazoles / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D012313:RNA / Q000737:chemistry*; D012323:RNA Processing, Post-Transcriptional; D014158:Transcription, Genetic
chemical_list: C528341:7-(2-thienyl)imidazo(4,5-b)pyridine; D007093:Imidazoles; D011725:Pyridines; D011758:Pyrroles; C051266:pyrrole-2-carboxaldehyde; D012313:RNA