title: 5-aza-CdR induces the demethylation of Syk promoter in nasopharyngeal carcinoma cell.
abstract: The present study employed 5-aza-2'-deoxycytidine (5-aza-CdR) to treat nasopharyngeal carcinoma cell line CNE-1, CNE-2 and non-cancerous human nasopharyngeal epithelial cell line NP-69 to understand the effects on spleen tyrosine kinase (Syk) gene promoter methylation. The results showed that the methylation level of Syk gene is negatively associated with the differentiation level of the cell lines, and the 5-aza-CdR treatment decreased the methylation level in nasopharyngeal carcinoma cell lines. Additionally, the drug sensitivity of low-differentiated cell line was significantly higher than the high-differentiated cell line. In conclusion, the Syk gene promoter methylation reflects the cell differentiation status, and 5-aza-CdR treatment could induce the demethylation of Syk gene promoter.
authors.affiliation: Department of Otolaryngology, Taizhou Municipal Hospital, Taizhou 318000, China.
authors.forename: Qiaozhi
authors.initials: Q
authors.lastname: Jin
authors.forename: Chibo
authors.initials: C
authors.lastname: Liu
authors.forename: Chong
authors.initials: C
authors.lastname: Yan
authors.forename: Baohong
authors.initials: B
authors.lastname: Tao
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Li
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Cai
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D001483:Base Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D019175:DNA Methylation; D017931:DNA Primers; D000077209:Decitabine; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000473:pathology*; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D000072377:Syk Kinase
chemical_list: D000964:Antimetabolites, Antineoplastic; D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D000077209:Decitabine; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase; D001374:Azacitidine