title: Detecting epigenetic changes: DNA methylation.
abstract: This unit provides protocols for assessing the state of DNA methylation, including assays for determining the overall DNA methylation levels, measuring DNA methyltransferase (MTase) activity, determining gene-specific methylation and overall chromatin condensation patterns.
authors.affiliation: New York University School of Medicine, New York, New York, USA.
authors.forename: L
authors.initials: L
authors.lastname: Broday
authors.forename: M
authors.initials: M
authors.lastname: Costa
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000187:drug effects*; D019175:DNA Methylation / Q000187:drug effects*; D044127:Epigenesis, Genetic / Q000187:drug effects*; D015870:Gene Expression / Q000187:drug effects; D020868:Gene Silencing / Q000187:drug effects*; D006801:Humans; D008780:Methyltransferases / Q000378:metabolism; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D002843:Chromatin; D015262:Xenobiotics; D004247:DNA; D008780:Methyltransferases