title: Rapid and selective screening of melamine in bovine milk using molecularly imprinted matrix solid-phase dispersion coupled with liquid chromatography-ultraviolet detection.
abstract: A simple, convenient and high selective molecularly imprinted matrix solid-phase dispersion (MI-MSPD) using water-compatible cyromazine-imprinted polymer as adsorbent was proposed for the rapid screening of melamine from bovine milk coupled with liquid chromatography-ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized by cyromazine as dummy template and reformative methanol-water system as reaction medium showed higher affinity and selectivity to melamine, and so they were applied as the specific dispersant of MSPD to extraction of melamine and simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.24-60.0μgg(-1) with the correlation coefficient of 0.9994. The recoveries of melamine at three spiked levels were ranged from 86.0 to 96.2% with the relative standard deviation (RSD)≤4.0%. This proposed MI-MSPD method combined the advantages of MSPD and MIPs, and could be used as an alternative tool for analyzing the residues of melamine in complex milk samples.
authors.affiliation: Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmacy, Hebei University, Baoding 071002, China. yanhongyuan@126.com
authors.forename: Hongyuan
authors.initials: H
authors.lastname: Yan
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Cheng
authors.forename: Ning
authors.initials: N
authors.lastname: Sun
authors.forename: Tianyu
authors.initials: T
authors.lastname: Cai
authors.forename: Ruijun
authors.initials: R
authors.lastname: Wu
authors.forename: Kun
authors.initials: K
authors.lastname: Han
mesh_terms: D000042:Absorption; D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016014:Linear Models; D000432:Methanol / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000032:analysis* / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D012997:Solvents; D014227:Triazines; D014867:Water; C028704:cyromazine; C011907:melamine; D000432:Methanol; C032159:acetonitrile