title: Use of dexmedetomidine to prevent pulmonary injury after pneumoperitoneum in ventilated rats.
abstract: OBJECTIVE
  This study examined the effectiveness of dexmedetomidine in preventing lung injury resulting from pneumoperitoneum in a ventilated rat model.
  
  
  METHODS
  Animals (n=18) were allocated randomly into 3 groups: control group, no pneumoperitoneum; sham group, pneumoperitoneum with intra-abdominal pressure of 12 mm Hg for 60 minutes; and dexmedetomidine group, dexmedetomidine administration 30 minutes before pneumoperitoneum. The rats were rested for 30 minutes after abdominal deflation. Then, blood samples were obtained for plasma malondialdehyde and ischemia-modified albumin (IMA) analyses. Tissue samples were taken for histopathologic examination and malondialdehyde analysis.
  
  
  RESULTS
  Compared with the control group, the sham group had a significantly higher level of plasma IMA. Pretreatment with dexmedetomidine significantly reduced the IMA level. Histopathologically, tissues from sham rats exhibited moderate or severe tissue damage, compared with control tissues. Dexmedetomidine-treated rats showed significantly less tissue damage than sham rats.
  
  
  CONCLUSIONS
  Dexmedetomidine prophylaxis resulted in significantly less IMA production and significantly less neutrophil infiltration, thereby helping to protect the lungs from injury after pneumoperitoneum.
authors.affiliation: Departments of Anesthesiology and Reanimation, Faculty of Medicine, Karadeniz Technical University, Farabi Hospital, Trabzon, Turkey. gezes@hotmail.com
authors.forename: Sukran
authors.initials: S
authors.lastname: Geze
authors.forename: Bahanur
authors.initials: B
authors.lastname: Cekic
authors.forename: Mustafa
authors.initials: M
authors.lastname: Imamoğlu
authors.forename: Mehmet Fatih
authors.initials: MF
authors.lastname: Yörük
authors.forename: Esin
authors.initials: E
authors.lastname: Yuluğ
authors.forename: Ahmet
authors.initials: A
authors.lastname: Alver
authors.forename: Ahmet
authors.initials: A
authors.lastname: Mentese
authors.forename: Engin
authors.initials: E
authors.lastname: Ertürk
authors.forename: Mustafa
authors.initials: M
authors.lastname: Tusat
mesh_terms: D058647:Adrenergic alpha-2 Receptor Agonists / Q000627:therapeutic use; D000818:Animals; D020927:Dexmedetomidine / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D055370:Lung Injury / Q000209:etiology / Q000517:prevention & control*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012121:Respiration, Artificial
chemical_list: D058647:Adrenergic alpha-2 Receptor Agonists; D020927:Dexmedetomidine