title: MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation.
abstract: A functional relationship between chromatin structure and mRNA processing events has been suggested, however, so far only a few involved factors have been characterized. Here we show that rsc nhp6ΔΔ mutants, deficient for the function of the chromatin remodeling factor RSC and the chromatin architectural proteins Nhp6A/Nhp6B, accumulate intron-containing pre-mRNA at the restrictive temperature. In addition, we demonstrate that rsc8-ts16 nhp6ΔΔ cells contain low levels of U6 snRNA and U4/U6 di-snRNA that is further exacerbated after two hours growth at the restrictive temperature. This change in U6 snRNA and U4/U6 di-snRNA levels in rsc8-ts16 nhp6ΔΔ cells is indicative of splicing deficient conditions. We identify MRN1 (multi-copy suppressor of rsc nhp6ΔΔ) as a growth suppressor of rsc nhp6ΔΔ synthetic sickness. Mrn1 is an RNA binding protein that localizes both to the nucleus and cytoplasm. Genetic interactions are observed between 2 µm-MRN1 and the splicing deficient mutants snt309Δ, prp3, prp4, and prp22, and additional genetic analyses link MRN1, SNT309, NHP6A/B, SWI/SNF, and RSC supporting the notion of a role of chromatin structure in mRNA processing.
authors.affiliation: Department of Biology, Copenhagen BioCenter, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Louis
authors.initials: L
authors.lastname: Düring
authors.forename: Michael
authors.initials: M
authors.lastname: Thorsen
authors.forename: Darima Sophia Njama
authors.initials: DS
authors.lastname: Petersen
authors.forename: Brian
authors.initials: B
authors.lastname: Køster
authors.forename: Torben Heick
authors.initials: TH
authors.lastname: Jensen
authors.forename: Steen
authors.initials: S
authors.lastname: Holmberg
mesh_terms: D042002:Chromatin Assembly and Disassembly / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D024001:HMGN Proteins / Q000235:genetics / Q000378:metabolism; D015151:Immunoblotting; D008856:Microscopy, Fluorescence; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D017381:Spliceosomes / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D024001:HMGN Proteins; C086471:NHP6A protein, S cerevisiae; C086472:NHP6B protein, S cerevisiae; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; C103337:RSC complex, S cerevisiae; C112008:SNT309 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C405482:U4 small nuclear RNA; C405486:U6 small nuclear RNA