title: [Effect of different solvents on extraction of effective components from Ligusticum chuanxiong].
abstract: OBJECTIVE
  To compare the effect of different solvents such as water, ethanol, methanol, ethyl acetate and petroleum ether on extraction of 10 effective components from Ligusticum chuanxiong and component characteristics of corresponding extracts.
  
  
  METHODS
  Ultrasonic assisted solvent extraction and high performance liquid chromatography quantitative analysis were adopted to determine effective components. CAPCELL PAK C18 column (4.6 mm x 150 mm, 5 microm) was adopted. The mobile phase was methanol-0. 5% HAc for gradient elute. The detection wavelength was 280 nm. The column temperature was 30 degrees C. The flow rate was 0. 7 mL x min(-1). The sample size was 10 microL.
  
  
  RESULTS
  Methanol or ethanol showed no significant difference in extraction of ferulic acid, hydroxyphthalide, alkylphthalide and diligustilide. Ethyl acetate displayed relatively low extraction ratios in hydroxyphthalide and ferulic acid. Water and petroleum ether showed relatively low extraction ratios in all four effective components, and water extracts showed different component characteristics.
  
  
  CONCLUSIONS
  Ethanol and methanol are the most suitable solvents to extract four effective components from L. chuanxiong.
authors.affiliation: School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China. yangxd@tju.edu.cn
authors.forename: Xuedong
authors.initials: X
authors.lastname: Yang
authors.forename: Xun
authors.initials: X
authors.lastname: Wu
authors.forename: Licui
authors.initials: L
authors.lastname: Hu
authors.forename: Henan
authors.initials: H
authors.lastname: Guo
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D030004:Ligusticum; D012997:Solvents / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D012997:Solvents; C101574:chuangxiong extract