title: In situ hybridization histochemistry.
abstract: This unit describes two methods of in situ hybridization: one uses an ³⁵S-labeled oligonucleotide probe and the other uses a digoxigenin-labeled oligonucleotide probe on frozen, cryostat-sectioned samples. These methods allow detection of the physical distribution and expression levels of target mRNA. Protocols are also included for labeling the probes and preparing the sample material.
authors.affiliation: University Hospital, Lund, Sweden.
authors.forename: Z
authors.initials: Z
authors.lastname: Kokaia
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002477:Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D017403:In Situ Hybridization; D015336:Molecular Probe Techniques; D009928:Organ Specificity; D012333:RNA, Messenger / Q000378:metabolism*; D015262:Xenobiotics / Q000633:toxicity
chemical_list: D012333:RNA, Messenger; D015262:Xenobiotics