title: Construction of a 4 zeptoliters switchable 3D DNA box origami.
abstract: The DNA origami technique is a recently developed self-assembly method that allows construction of 3D objects at the nanoscale for various applications. In the current study we report the production of a 18 × 18 × 24 nm(3) hollow DNA box origami structure with a switchable lid. The structure was efficiently produced and characterized by atomic force microscopy, transmission electron microscopy, and Förster resonance energy transfer spectroscopy. The DNA box has a unique reclosing mechanism, which enables it to repeatedly open and close in response to a unique set of DNA keys. This DNA device can potentially be used for a broad range of applications such as controlling the function of single molecules, controlled drug delivery, and molecular computing.
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, Denmark.
authors.forename: Reza M
authors.initials: RM
authors.lastname: Zadegan
authors.forename: Mette D E
authors.initials: MD
authors.lastname: Jepsen
authors.forename: Karen E
authors.initials: KE
authors.lastname: Thomsen
authors.forename: Anders H
authors.initials: AH
authors.lastname: Okholm
authors.forename: David H
authors.initials: DH
authors.lastname: Schaffert
authors.forename: Ebbe S
authors.initials: ES
authors.lastname: Andersen
authors.forename: Victoria
authors.initials: V
authors.lastname: Birkedal
authors.forename: Jørgen
authors.initials: J
authors.lastname: Kjems
mesh_terms: D003460:Crystallization / Q000379:methods*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure*; D046911:Macromolecular Substances / Q000737:chemistry; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D009690:Nucleic Acid Conformation; D010316:Particle Size; D013499:Surface Properties
chemical_list: D046911:Macromolecular Substances; D004247:DNA