title: Immunomodulatory potential of partially hydrolyzed β-lactoglobulin and large synthetic peptides.
abstract: The immunomodulatory potential of fragments derived from the cow's milk allergen bovine β-lactoglobulin (BLG) was assessed in a mouse model of oral tolerance (OT) [Adel-Patient, K.; Wavrin, S.; Bernard, H.; Meziti, N.; Ah-Leung, S.; Wal, J. M. Oral tolerance and Treg cells are induced in BALB/c mice after gavage with bovine β-lactoglobulin. Allergy 2011, 66 (10), 1312-1321]. Native BLG (nBLG) and chemically denatured BLG (lacking S-S bridges, dBLG), products resulting from their hydrolysis using cyanogen bromide (CNBr) and some synthetic peptides, were produced and precisely characterized. CNBr hydrolysates correspond to pools of peptides of various sizes that are still associated by S-S bridges when derived from nBLG. nBLG, dBLG, and CNBr hydrolysate of nBLG efficiently prevented further sensitization. CNBr hydrolysate of dBLG was less efficient, suggesting that the association by S-S bridges of peptides increased their immunomodulatory potential. Conversely, synthetic peptides were inefficient even if covering 50% of the BLG sequence, demonstrating that the immunomodulatory potential requires the presence of all derived fragments of BLG and further supporting the use of partially hydrolyzed milk proteins to favor OT induction in infants with a risk of atopy.
authors.affiliation: INRA, UR496 Immuno-Allergie Alimentaire, CEA/IBiTeC-S/SPI, CEA de Saclay, F-91191 Gif sur Yvette cedex, France. karine.adel-patient@cea.fr
authors.forename: Karine
authors.initials: K
authors.lastname: Adel-Patient
authors.forename: Sophie
authors.initials: S
authors.lastname: Nutten
authors.forename: Hervé
authors.initials: H
authors.lastname: Bernard
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Fritsché
authors.forename: Sandrine
authors.initials: S
authors.lastname: Ah-Leung
authors.forename: Narimane
authors.initials: N
authors.lastname: Meziti
authors.forename: Guénolée
authors.initials: G
authors.lastname: Prioult
authors.forename: Annick
authors.initials: A
authors.lastname: Mercenier
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Wal
mesh_terms: D000818:Animals; D002417:Cattle; D003488:Cyanogen Bromide / Q000737:chemistry; D005260:Female; D005512:Food Hypersensitivity / Q000178:diet therapy / Q000276:immunology*; D006801:Humans; D006868:Hydrolysis; D007108:Immune Tolerance; D007782:Lactoglobulins / Q000737:chemistry* / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology* / Q000378:metabolism; D011492:Protein Hydrolysates / Q000276:immunology / Q000378:metabolism
chemical_list: D007782:Lactoglobulins; D010455:Peptides; D011492:Protein Hydrolysates; D003488:Cyanogen Bromide