title: Patients with activated B-cell like diffuse large B-cell lymphoma in high and low infectious disease areas have different inflammatory gene signatures.
abstract: Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous disease with an association with inflammation and viral infections. We hypothesize that environmental factors may be involved in the pathogenesis of DLBCL. In this study, we compared gene expression profiles of lymph node tissues from patients with DLBCL from two different geographical areas with diverse environmental exposures. Specimens from Egyptian and Swedish patients with DLBCL as well as controls were studied. Gene expression analysis using microarray and quantitative polymerase chain reaction demonstrated significantly higher expression of signal transducer and activator of transcription 3 (STAT3) in Swedish as compared to Egyptian patients and control materials from both countries. This was confirmed at protein level using confocal microscopy. The receptor tyrosine kinase ROR1, a "survival factor" for malignant cells, was overexpressed and significantly related to the STAT3 expression pattern. The difference in the expression of genes involved in inflammatory responses and in the tumorigenic process of DLBCL might relate to infectious agents and/or other environmental exposures.
authors.affiliation: Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Therese
authors.initials: T
authors.lastname: Högfeldt
authors.forename: Abeer A
authors.initials: AA
authors.lastname: Bahnassy
authors.forename: Anna
authors.initials: A
authors.lastname: Kwiecinska
authors.forename: Anders
authors.initials: A
authors.lastname: Osterborg
authors.forename: Katja Pokrovskaja
authors.initials: KP
authors.lastname: Tamm
authors.forename: Anna
authors.initials: A
authors.lastname: Porwit
authors.forename: Abdel-Rahman N
authors.initials: AR
authors.lastname: Zekri
authors.forename: Joachim
authors.initials: J
authors.lastname: Lundahl
authors.forename: Hussein M
authors.initials: HM
authors.lastname: Khaled
authors.forename: Håkan
authors.initials: H
authors.lastname: Mellstedt
authors.forename: Ali
authors.initials: A
authors.lastname: Moshfegh
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D004534:Egypt; D004777:Environment; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D059647:Gene-Environment Interaction; D006801:Humans; D007249:Inflammation / Q000150:complications* / Q000235:genetics; D008213:Lymphocyte Activation / Q000235:genetics; D016403:Lymphoma, Large B-Cell, Diffuse / Q000150:complications* / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D057050:Receptor Tyrosine Kinase-like Orphan Receptors / Q000235:genetics; D050791:STAT Transcription Factors / Q000235:genetics; D015398:Signal Transduction; D013548:Sweden; D014777:Virus Diseases / Q000150:complications* / Q000235:genetics
chemical_list: D050791:STAT Transcription Factors; C078716:ROR1 protein, human; D057050:Receptor Tyrosine Kinase-like Orphan Receptors