title: Quantitation of human heme oxygenase (HO-1) copies by competitive RT-PCR.
abstract: The authors have developed a system for quantifying human HO-1 mRNA in samples limited in cell number and/or mRNA copies. Total RNA from human liver was used to develop the system. The RNA is reverse transcribed and amplified by PCR in a tube also containing an internal standard obtained by deleting 50 base pairs from the original human gene. After amplification the two templates are resolved and quantified. When the internal standard is present in the reaction mixture, the ratio of amplified sample to internal standard is proportional to the amount of sample RNA making it possible to calculate the number of specific mRNA molecules.
authors.affiliation: New York Medical College, Valhalla, NY, USA.
authors.forename: N G
authors.initials: NG
authors.lastname: Abraham
authors.forename: S K
authors.initials: SK
authors.lastname: Shenouda
authors.forename: A
authors.initials: A
authors.lastname: Goodman
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D051547:Heme Oxygenase-1 / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D051547:Heme Oxygenase-1