title: Detection of dengue virus 4 from central India.
abstract: OBJECTIVE
  Dengue is an important arboviral disease. All four dengue virus serotypes are reported to be circulating in India. It is also known that different serotypes, genotypes and clades of genotype determine outbreak severity. Dengue affected children are known to have serious disease outcome. We carried out this study to give reliable diagnosis of dengue infection in children and to detect circulating serotype in central India.
  
  
  METHODS
  Samples collected from paediatric patients suspected to have dengue fever were subjected to IgM and IgG ELISA to determine dengue virus infection. Samples collected within 0-5 days of onset of illness and positive by IgM ELISA were tested by nested reverse transcription polymerase chain reaction (nRT-PCR). The PCR products were sequenced and analyzed.
  
  
  RESULTS
  Of the 89 samples tested, 18 and 7 were positive for dengue IgM and IgG, respectively. Dengue activity was observed in both Jabalpur city and adjoining rural settings. One sample found positive by nRT-PCR was further sequenced to confirm dengue virus 4 as aetiological agent.
  
  
  CONCLUSIONS
  Our findings demonstrated dengue virus infection in children and adolescent in central India. Because of continuous changing epidemiology, it is important to monitor dengue virus activity at both serological and molecular level in this part of the country for better patient care and management.
authors.affiliation: Regional Medical Research Centre for Tribals (ICMR), Nagpur Road, Garha, Jabalpur, India.
authors.forename: P V
authors.initials: PV
authors.lastname: Barde
authors.forename: S
authors.initials: S
authors.lastname: Godbole
authors.forename: P K
authors.initials: PK
authors.lastname: Bharti
authors.forename: Gyan
authors.initials: G
authors.lastname: Chand
authors.forename: M
authors.initials: M
authors.lastname: Agarwal
authors.forename: Neeru
authors.initials: N
authors.lastname: Singh
mesh_terms: D000293:Adolescent; D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002675:Child, Preschool; D003716:Dengue Virus / Q000302:isolation & purification*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D007194:India; D007223:Infant; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M