title: Alterations of mitochondrial protein assembly and jasmonic acid biosynthesis pathway in Honglian (HL)-type cytoplasmic male sterility rice.
abstract: It has been suggested that the mitochondrial chimeric gene orfH79 is the cause for abortion of microspores in Honglian cytoplasmic male sterile rice, yet little is known regarding its mechanism of action. In this study, we used a mass spectrometry-based quantitative proteomics strategy to compare the mitochondrial proteome between the sterile line Yuetai A and its fertile near-isogenic line Yuetai B. We discovered a reduced quantity of specific proteins in mitochondrial complexes in Yuetai A compared with Yuetai B, indicating a defect in mitochondrial complex assembly in the sterile line. Western blotting showed that ORFH79 protein and ATP1 protein, an F(1) sector component of complex V, are both associated with large protein complexes of similar size. Respiratory complex activity assays and transmission electron microscopy revealed functional and morphological defects in the mitochondria of Yuetai A when compared with Yuetai B. In addition, we identified one sex determination TASSELSEED2-like protein increased in Yuetai A, leading to the discovery of an aberrant variation of the jasmonic acid pathway during the development of microspores.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Gai
authors.initials: G
authors.lastname: Liu
authors.forename: Han
authors.initials: H
authors.lastname: Tian
authors.forename: Yun-Qing
authors.initials: YQ
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.forename: Yan-Xiao
authors.initials: YX
authors.lastname: Ji
authors.forename: Shao-Qing
authors.initials: SQ
authors.lastname: Li
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
authors.forename: Ying-Guo
authors.initials: YG
authors.lastname: Zhu
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism*; D051479:Plant Infertility; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011058:Pollen / Q000235:genetics / Q000378:metabolism*; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism
chemical_list: D003517:Cyclopentanes; D024101:Mitochondrial Proteins; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; D006180:Proton-Translocating ATPases