title: Short- and long-term changes in blood miRNA levels after nanogold injection in rats--potential biomarkers of nanoparticle exposure.
abstract: BACKGROUND
  Increased use of engineered nanoparticles may result in exposure of workers and consumers, making them a health concern.
  
  
  OBJECTIVE
  To identify potential blood miRNA biomarkers after intravenous gold nanoparticle (AuNP) exposure.
  
  
  METHODS
  miRNA microarray analysis was carried out on blood of rats at 1 week and 2 months after injection.
  
  
  RESULTS
  Many up- and downregulated miRNAs were detected. Of these, rno-miR-298 was confirmed to be increased at 1 week postinjection by reverse transcription-PCR (RT-PCR).
  
  
  CONCLUSIONS
  Blood miRNAs could be useful as biomarkers for exposure to nanoparticles. miR-298 regulates β-amyloid (Aβ) precursor protein-converting enzyme-1 (BACE1) in Alzheimer's disease.
authors.affiliation: Neurobiology and Ageing Research Programme, National University of Singapore, Singapore 119260.
authors.forename: Wee-Siong
authors.initials: WS
authors.lastname: Chew
authors.forename: Kay-Wee
authors.initials: KW
authors.lastname: Poh
authors.forename: Nikhat J
authors.initials: NJ
authors.lastname: Siddiqi
authors.forename: Abdullah S
authors.initials: AS
authors.lastname: Alhomida
authors.forename: Liya E
authors.initials: LE
authors.lastname: Yu
authors.forename: Wei-Yi
authors.initials: WY
authors.lastname: Ong
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D006046:Gold / Q000737:chemistry*; D007275:Injections, Intravenous; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D015415:Biomarkers; C581176:MIRN298 microRNA, rat; D035683:MicroRNAs; D006046:Gold