title: Expressional analysis of immunoglobulin D in cattle (Bos taurus), a large domesticated ungulate.
abstract: For decades, it has remained unknown whether artiodactyls, such as cattle, pigs, and sheep, express immunoglobulin D (IgD), although the δ gene was identified in these species nearly 10 years ago. By developing a mouse anti-bovine IgD heavy chain monoclonal antibody (13C2), we show that secreted bovine IgD was present mainly as a monomer in serum and was heavily glycosylated by N-linked saccharides. Nonetheless, IgD was detectable in some but not all of the Holstein cattle examined. Membrane-bound IgD was detected in the spleen by western blotting. Flow cytometric analysis demonstrated that IgD-positive B cells constituted a much lower percentage of B cells in the bovine spleen (∼6.8% of total B cells), jejunal Peyer's patches (∼0.8%), and peripheral blood leukocytes (∼1.2%) than in humans and mice. Furthermore, IgD-positive B cells were almost undetectable in bovine bone marrow and ileal Peyer's patches. We also demonstrated that the bovine δ gene can be expressed via class switch recombination. Accordingly, bovine δ germline transcription, which involves an Iδ exon and is highly homologous to Iμ, was confirmed. However, we could not identify an Iδ promoter, despite bovine Eμ demonstrating both enhancer and promoter activity. This study has answered a long-standing question in cattle B cell biology and significantly contributes to our understanding of B cell development in this species.
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Sciences, National Engineering Laboratory for Animal Breeding, China Agricultural University, Beijing, PR China.
authors.forename: Beilei
authors.initials: B
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Wei
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Qiqing
authors.initials: Q
authors.lastname: Tao
authors.forename: Liming
authors.initials: L
authors.lastname: Ren
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Hu
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: Jing
authors.initials: J
authors.lastname: Fei
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Yaofeng
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D015153:Blotting, Western; D002417:Cattle; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007072:Immunoglobulin D / Q000235:genetics*; D010581:Peyer's Patches / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013154:Spleen / Q000378:metabolism
chemical_list: D007072:Immunoglobulin D