title: Chd1 chromatin remodelers maintain nucleosome organization and repress cryptic transcription.
abstract: Chromatin organization is essential for defining transcription units and maintaining genomic integrity in eukaryotes. In this study, we found that deletion of the Schizosaccharomyces pombe Chd1 chromatin remodelers, hrp1 and hrp3, causes strong, genome-wide accumulation of antisense transcripts. Nucleosome mapping revealed a specific role for Chd1 remodelers in the positioning of nucleosomes in gene coding regions. Other mutations associated with enhanced cryptic transcription activity, such as set2Δ, alp13Δ and FACT complex subunit pob3Δ, did not, or only mildly, affect nucleosome positioning. These data indicate several mechanisms in the repression of cryptic promoter activity in eukaryotic cells.
authors.affiliation: Biochemistry Center, BZH, Heidelberg University, Im Neuenheimer Feld 328, Heidelberg 69120, Germany.
authors.forename: Bianca P
authors.initials: BP
authors.lastname: Hennig
authors.forename: Katja
authors.initials: K
authors.lastname: Bendrin
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Tamás
authors.initials: T
authors.lastname: Fischer
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics; D042002:Chromatin Assembly and Disassembly; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D016681:Genome, Fungal; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics; D009707:Nucleosomes / Q000737:chemistry* / Q000378:metabolism; D016372:RNA, Antisense / Q000096:biosynthesis; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D056488:Histone Chaperones
chemical_list: D000251:Adenosine Triphosphatases; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004265:DNA Helicases; D004268:DNA-Binding Proteins; D011495:Histone-Lysine N-Methyltransferase; D009707:Nucleosomes; D016372:RNA, Antisense; D029702:Schizosaccharomyces pombe Proteins; D014157:Transcription Factors; C475479:Alp13 protein, S pombe; C581763:POB3 protein, S pombe; D056488:Histone Chaperones; C527202:Set2 protein, S pombe; C464653:Hrp3 protein, S pombe; C111321:hrp1 protein, S pombe; C581762:Chd1 protein, S pombe