title: Pandemic influenza A/H1N1 virus in a swine farm house in Sicily, Italy.
abstract: This report describes a pandemic A/H1N1 (H1N1 pdm) virus outbreak occurred in December, 2009 in a swine farm used as research facility (Istituto Mediterraneo Trapianti e Terapie ad Alta Specializzazione) for preclinical studies, located in Sicily, Italy. All the 13 pigs of the farm, showed cough, fever, inappetence and weakness. At the same time, an unvaccinated worker of the stabling showed influenza-like symptoms. RNAv extracted from two swabs collected from infected pigs resulted positive by Real Time RT-PCR for Influenza A virus. Furthermore, after growth on embryonated eggs, viral isolates were identified by Real Time RT-PCR specific for H1N1 pdm virus and characterized antigenically. Sequencing of the whole genome was also performed. All sera taken from animals and from the worker were tested by a competitive influenza A ELISA and by the haemoagglutination inhibition test. Serological findings confirmed the circulation of influenza virus H1N1 pdm in pigs and the presence of specific antibodies against H1N1 pdm in human serum. The results of this study seem to support a H1N1 pdm transmission from man to animals showing the importance of serological and virological investigation to control the pig farms and the importance of close cooperation between the different authorities like veterinarian and human public.
authors.affiliation: Zooprofilattico Sperimentale d ella Sicilia, 90129 Palermo, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Guercio
authors.forename: G
authors.initials: G
authors.lastname: Purpari
authors.forename: P G
authors.initials: PG
authors.lastname: Conaldi
authors.forename: V
authors.initials: V
authors.lastname: Pagano
authors.forename: A
authors.initials: A
authors.lastname: Moreno
authors.forename: P
authors.initials: P
authors.lastname: Giambruno
authors.forename: L
authors.initials: L
authors.lastname: Di Trani
authors.forename: G
authors.initials: G
authors.lastname: Vaccari
authors.forename: E
authors.initials: E
authors.lastname: Falcone
authors.forename: A Boni
authors.initials: AB
authors.lastname: Istituto
authors.forename: P
authors.initials: P
authors.lastname: Cordioli
mesh_terms: D000818:Animals; D004196:Disease Outbreaks / Q000662:veterinary; D005260:Female; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification*; D007251:Influenza, Human / Q000453:epidemiology / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D058873:Pandemics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012802:Sicily / Q000453:epidemiology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*