title: Low prevalence of Chagas parasite infection in a nonhuman primate colony in Louisiana.
abstract: Chagas disease, an important cause of heart disease in Latin America, is caused by the parasite Trypanosoma cruzi, which typically is transmitted to humans by triatomine insects. Although autochthonous transmission of the Chagas parasite to humans is rare in the United States, triatomines are common, and more than 20 species of mammals are infected with the Chagas parasite in the southern United States. Chagas disease has also been detected in colonies of nonhuman primates (NHP) in Georgia and Texas, and heart abnormalities consistent with Chagas disease have occurred at our NHP center in Louisiana. To determine the level of T. cruzi infection, we serologically tested 2157 of the approximately 4200 NHP at the center; 34 of 2157 primates (1.6%) tested positive. Presence of the T. cruzi parasite was confirmed by hemoculture in 4 NHP and PCR of the cultured parasites. These results strongly suggest local transmission of T. cruzi, because most of the infected NHP were born and raised at this site. All 3 species of NHP tested yielded infected animals, with significantly higher infection prevalence in pig-tailed macaques, suggesting possible exploration of this species as a model organism. The local T. cruzi strain isolated during this study would enhance such investigations. The NHP at this center are bred for use in scientific research, and the effects of the Chagas parasite on infected primates could confuse the interpretation of other studies.
authors.affiliation: Loyola University New Orleans, New Orleans, USA. dorn@loyno.edu
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Dorn
authors.forename: Megan E
authors.initials: ME
authors.lastname: Daigle
authors.forename: Crescent L
authors.initials: CL
authors.lastname: Combe
authors.forename: Ashley H
authors.initials: AH
authors.lastname: Tate
authors.forename: Lori
authors.initials: L
authors.lastname: Stevens
authors.forename: Kathrine M
authors.initials: KM
authors.lastname: Phillippi-Falkenstein
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D014355:Chagas Disease / Q000453:epidemiology / Q000635:transmission / Q000662:veterinary*; D016054:DNA, Protozoan / Q000097:blood; D008145:Louisiana / Q000453:epidemiology; D008254:Macaca nemestrina / Q000469:parasitology; D008297:Male; D018512:Parasitemia / Q000469:parasitology; D016133:Polymerase Chain Reaction; D018419:Primate Diseases / Q000453:epidemiology* / Q000469:parasitology*; D011323:Primates / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000913:Antibodies, Protozoan; D016054:DNA, Protozoan