title: Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy.
abstract: We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed inherent to OCM. We report on the detection of single 40 nm gold particles with a 0.5 μm lateral and 2 μm axial resolution over a 50 μm depth of field and the three-dimensional localization of gold colloids within living cells. In combination with intrinsic sample contrast measured with dark-field OCM, poli-OCM offers a versatile platform for functional cell imaging.
authors.affiliation: Laboratoire d’Optique Biom´edicale, Ecole Polytechnique F´ed´erale de Lausanne, Lausanne, Switzerland. christophe.pache@epfl.ch
authors.forename: Christophe
authors.initials: C
authors.lastname: Pache
authors.forename: Noelia L
authors.initials: NL
authors.lastname: Bocchio
authors.forename: Arno
authors.initials: A
authors.lastname: Bouwens
authors.forename: Martin
authors.initials: M
authors.lastname: Villiger
authors.forename: Corinne
authors.initials: C
authors.lastname: Berclaz
authors.forename: Joan
authors.initials: J
authors.lastname: Goulley
authors.forename: Matthew I
authors.initials: MI
authors.lastname: Gibson
authors.forename: Christian
authors.initials: C
authors.lastname: Santschi
authors.forename: Theo
authors.initials: T
authors.lastname: Lasser
mesh_terms: D002470:Cell Survival; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008853:Microscopy / Q000379:methods*; D055620:Optical Phenomena; D059629:Signal-To-Noise Ratio; D013696:Temperature
chemical_list: D004129:Dimethylpolysiloxanes; D006046:Gold