title: Isolation of potential probiotic Lactobacillus rhamnosus strains from traditional fermented mare milk produced in Sumbawa Island of Indonesia.
abstract: To explore potential probiotics in the traditional foods of Indonesia, fermented mare milk produced in Sumbawa Island was investigated in this study. Gram stain, catalase activity, gas production, cell morphology, carbohydrate utilization pattern, and 16S rDNA sequencing were performed to identify isolated lactic acid bacteria. To assess their probiotic ability, tolerance of low pH, bile salts, artificial gastrointestinal fluids, and adhesion properties to extracellular matrices, were examined. In total 27 strains, 25 Lactobacillus rhamnosus and two Lactobacillus fermentum, were obtained. Among the isolated lactobacilli, three Lb. rhamnosus strains, FSMM15, FSMM22, and FSMM26, were selected as candidates for probiotics, using Lb. rhamnosus GG as index. In vitro binding assay of the three strains against several extracellular matrix proteins revealed that FSMM15 and FSMM26 gave greater binding ratios of mucin/bovine serum albumin (BSA) and significantly higher adhesive abilities to fibronectin than Lb. rhamnosus GG. FSMM22 showed significantly higher adhesion to laminin than Lb. rhamnosus GG.
authors.affiliation: Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
authors.forename: Tala
authors.initials: T
authors.lastname: Shi
authors.forename: Keita
authors.initials: K
authors.lastname: Nishiyama
authors.forename: Koichi
authors.initials: K
authors.lastname: Nakamata
authors.forename: Ni Putu Desy
authors.initials: NP
authors.lastname: Aryantini
authors.forename: Dai
authors.initials: D
authors.lastname: Mikumo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Oda
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Takao
authors.initials: T
authors.lastname: Mukai
authors.forename: I Nengah
authors.initials: IN
authors.lastname: Sujaya
authors.forename: Tadasu
authors.initials: T
authors.lastname: Urashima
authors.forename: Kenji
authors.initials: K
authors.lastname: Fukuda
mesh_terms: D000818:Animals; D005260:Female; D005285:Fermentation; D006736:Horses / Q000378:metabolism*; D007214:Indonesia; D062312:Islands; D052201:Lacticaseibacillus rhamnosus / Q000302:isolation & purification*; D008892:Milk / Q000378:metabolism*; D019936:Probiotics / Q000302:isolation & purification*