title: Purification and characterization of heme oxygenase.
abstract: High-yield expression and purification of human heme oxygenase isozyme 1 provided the breakthrough in characterizing the protein from mechanistic and structural standpoints. This unit provides a protocol for high-level expression and subsequent purification of HO-1. The commentary includes a discussion of subsequent biochemical and biophysical characterizations.
authors.affiliation: University of Maryland, Baltimore, MD. USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Wilks
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D051547:Heme Oxygenase-1; D009251:NADPH-Ferrihemoprotein Reductase