title: Pseudomonas fluorescens N21.4 metabolites enhance secondary metabolism isoflavones in soybean (Glycine max) calli cultures.
abstract: Phytopharmaceuticals are plant secondary metabolites that are strongly inducible and especially sensitive to biotic changes. Plant cell cultures are a good alternative to obtain secondary metabolites, in case effective stimulation can be achieved. In this study, metabolic elicitors from two rhizobacteria able to enhance isoflavone content in soybean seedlings were tested on three different soybean calli cell lines. Results show that metabolic elicitors from  Chryseobacterium balustinum  Aur9 were not effective. However, there are at least two different metabolic elicitors from  Pseudomonas fluorescens  N21.4, one under 10 kDa and another over 10 kDa, that trigger isoflavone metabolism in the three cell lines with different isoflavone content. Elicitors from N21.4 achieved total isoflavone increases up to 29.7% (0.205 mg/g), 64.5% (0.487 mg/g), and 23.4% (0.726 mg/g) in the low-, intermediate-, and high-yield lines, respectively. Therefore, these elicitors have a great potential to enhance isoflavone production in cell cultures for development of functional ingredients.
authors.affiliation: Facultad de Farmacia, Universidad San Pablo CEU, Ctra. Boadilla del Monte km 5.3, 28668 Madrid, Spain. elalgar@ceu.es
authors.forename: Elena
authors.initials: E
authors.lastname: Algar
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Gutierrez-Mañero
authors.forename: Alfonso
authors.initials: A
authors.lastname: Bonilla
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Lucas
authors.forename: Wojtek
authors.initials: W
authors.lastname: Radzki
authors.forename: Beatriz
authors.initials: B
authors.lastname: Ramos-Solano
mesh_terms: D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D007529:Isoflavones / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D013025:Glycine max / Q000166:cytology / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D007529:Isoflavones