title: Purification of baicalin and wogonoside from Scutellaria baicalensis extracts by macroporous resin adsorption chromatography.
abstract: In this study, resin adsorption as a means to separate and purify baicalin and wogonoside from extracts of Scutellaria baicalensis was investigated. Among the ten tested resins, the non-polar resin HPD-100 offered the best adsorption and desorption properties. Langmuir and Freundlich isotherms were used to describe the interactions between solutes and resin at different temperatures, and the equilibrium experimental data were well fitted to the two isotherms. Column packed with HPD-100 resin was used to perform dynamic adsorption and desorption tests to optimize the separation process. After one round treatment with HPD-100 resin, the contents of baicalin and wogonoside were 3.6-fold and 12.0-fold increased with recovery yields of 85.7% and 65.6%, respectively. In addition, a laboratory preparative-scale separation was carried out under the final conditions. The results showed that the preparative separation of baicalin and wogonoside can be easily and efficiently achieved via adsorption and desorption on HPD-100 resin. The developed method is a promising basis for large-scale preparation of baicalin and wogonoside from S. baicalensis extracts.
authors.affiliation: Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Zhanquan
authors.initials: Z
authors.lastname: Du
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tao
authors.forename: Lixia
authors.initials: L
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D044950:Flavanones / Q000737:chemistry / Q000302:isolation & purification*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry*; D013696:Temperature
chemical_list: D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract; C038044:baicalin; D000431:Ethanol; C473995:wogonoside