title: Induction of motor neuron differentiation by transduction of Olig2 protein.
abstract: Olig2 protein, a member of the basic helix-loop-helix transcription factor family, was introduced into the mouse embryonic carcinoma cell line P19 for induction of motor neuron differentiation. We show that Olig2 protein has the ability to permeate the cell membrane without the addition of a protein transduction domain (PTD), similar to other basic helix-loop-helix transcription factors such as MyoD and NeuroD2. Motor neuron differentiation was evaluated for the elongation of neurites and the expression of choline acetyltransferase (ChAT) mRNA, a differentiation marker of motor neurons. By addition of Olig2 protein, motor neuron differentiation was induced in P19 cells.
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Mie
authors.forename: Mami
authors.initials: M
authors.lastname: Kaneko
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Henmi
authors.forename: Eiry
authors.initials: E
authors.lastname: Kobatake
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002795:Choline O-Acetyltransferase / Q000096:biosynthesis; D051379:Mice; D009046:Motor Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D016501:Neurites / Q000378:metabolism / Q000502:physiology; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D000076104:Oligodendrocyte Transcription Factor 2; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002795:Choline O-Acetyltransferase; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D012333:RNA, Messenger; C512773:Olig2 protein, mouse