title: Melghirimyces thermohalophilus sp. nov., a thermoactinomycete isolated from an Algerian salt lake.
abstract: A novel filamentous bacterium, designated Nari11A(T), was isolated from soil collected from a salt lake named Chott Melghir, located in north-eastern Algeria. The strain is an aerobic, halophilic, thermotolerant, Gram-stain-positive bacterium, growing at NaCl concentrations between 5 and 20 % (w/v) and at 43-60 °C and pH 5.0-10.0. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0. The DNA G+C content was 53.4 mol%. ll-Diaminopimelic acid was the diamino acid of the peptidoglycan. The major menaquinone was MK-7, but MK-6 and MK-8 were also present in trace amounts. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and three unidentified phospholipids. Results of molecular and phenotypic analyses led to the description of the strain as a novel member to the genus Melghirimyces, family Thermoactinomycetaceae. Strain Nari11A(T) shows 96.7 % 16S rRNA gene sequence similarity to the type strain of Melghirimyces algeriensis. On the basis of phenotypic, physiological and phylogenetic data, strain Nari11A(T) ( = DSM 45514(T)  = CCUG 60050(T)) represents the type strain of a novel species, for which the name Melghirimyces thermohalophilus sp. nov. is proposed.
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire (équipe de Microbiologie), Université des sciences et de la technologie, Houari Boumédiènne, Bab Ezzouar, Algiers, Algeria.|Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288 Marseille Cedex 09, France.
authors.forename: Ammara Nariman
authors.initials: AN
authors.lastname: Addou
authors.affiliation: Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire (équipe de Microbiologie), Université des sciences et de la technologie, Houari Boumédiènne, Bab Ezzouar, Algiers, Algeria.
authors.forename: Amel
authors.initials: A
authors.lastname: Bouanane-Darenfed
authors.affiliation: Centre de Recherche Nucléaire d'Alger (CRNA), Algeria.
authors.forename: Samia
authors.initials: S
authors.lastname: Amarouche-Yala
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire (équipe de Microbiologie), Université des sciences et de la technologie, Houari Boumédiènne, Bab Ezzouar, Algiers, Algeria.
authors.forename: Hocine
authors.initials: H
authors.lastname: Hacene
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288 Marseille Cedex 09, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.affiliation: Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288 Marseille Cedex 09, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
mesh_terms: D000462:Algeria; D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000032:analysis; D024482:Vitamin K 2 / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D024482:Vitamin K 2