title: Multiple pollution biomarker application on tissues of Eobania vermiculata during two periods characterized by augmented and reduced snail activity.
abstract: In the present study a package of biomarkers was applied on land snails E. vermiculata collected from polluted areas, as well as from an unpolluted reference one. Snail collection was performed during two different sampling periods characterized by reduced and augmented organism activity, October and May, respectively. The biomarkers applied were lysosomal membrane stability on digestive cells (LMS), neutral red lysosomal retention assay on haemocytes (NRR), morphometric changes of the lysosomal system (VDL, NDL), morphometric alterations of the neutral lipids (VDLP, NDLP), acetylcholinesterase activity on digestive gland and hemolymph (AChE), metallothionein content on digestive gland (MTs) and cyclic AMP content on digestive gland (cAMP). The results revealed significant differences in biomarker values between the two sampling periods. Significant differences were also detected among the sampling groups. The fluctuation of the parameters applied indicated that spring is a more suitable period for sampling conduction compared to autumn and that biomonitoring studies should be performed with special attention during the last mentioned period.
authors.affiliation: Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Itziou
authors.forename: V K
authors.initials: VK
authors.lastname: Dimitriadis
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D049851:Gastropoda / Q000187:drug effects*; D006434:Hemocytes / Q000187:drug effects / Q000201:enzymology; D007425:Intracellular Membranes / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D008247:Lysosomes / Q000187:drug effects; D008668:Metallothionein / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D009499:Neutral Red / Q000378:metabolism; D012621:Seasons; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D008055:Lipids; D008670:Metals; D014874:Water Pollutants, Chemical; D009499:Neutral Red; D008668:Metallothionein; D000110:Acetylcholinesterase