title: Large-scale forward genetic screening analysis of development of hematopoiesis in zebrafish.
abstract: Zebrafish is a powerful model for the investigation of hematopoiesis. In order to isolate novel mutants with hematopoietic defects, large-scale mutagenesis screening of zebrafish was performed. By scoring specific hematopoietic markers, 52 mutants were identified and then classified into four types based on specific phenotypic traits. Each mutant represented a putative mutation of a gene regulating the relevant aspect of hematopoiesis, including early macrophage development, early granulopoiesis, embryonic myelopoiesis, and definitive erythropoiesis/lymphopoiesis. Our method should be applicable for other types of genetic screening in zebrafish. In addition, further study of the mutants we identified may help to unveil the molecular basis of hematopoiesis.
authors.affiliation: Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Guangdong Higher Education Institutes, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Huang
authors.forename: Lingfeng
authors.initials: L
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Meng
authors.forename: Qing
authors.initials: Q
authors.lastname: Lin
authors.forename: Yali
authors.initials: Y
authors.lastname: Chi
authors.forename: Mengchang
authors.initials: M
authors.lastname: Xu
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Yiyue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Wenqing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000502:physiology; D005038:Ethylnitrosourea / Q000633:toxicity; D018507:Gene Expression Regulation, Developmental; D005820:Genetic Testing; D006410:Hematopoiesis / Q000235:genetics / Q000502:physiology; D016296:Mutagenesis; D009154:Mutation / Q000187:drug effects* / Q000502:physiology; D038042:Myelopoiesis; D010641:Phenotype; D015027:Zebrafish / Q000196:embryology / Q000235:genetics
chemical_list: D005038:Ethylnitrosourea