title: Detection of biliverdin reductase activity.
abstract: The conversion of biliverdin to the bile pigment bilirubin is catalyzed by biliverdin reductase, a cytosolic enzyme with two pH optima with different cofactors. The enzyme is assayed at pH 8.7 with NADPH as a cofactor and at 6.75 with NADH. The production of bilirubin is detected as described in this unit with a spectrophotometric assay. The enzyme can be qualitatively assayed by immunoblotting and changes in reductase isoform expression can be detected by two-dimensional electrophoresis.
authors.affiliation: New York Blood Center, New York, NY, USA.
authors.forename: Tian-Jun
authors.initials: TJ
authors.lastname: Huang
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D008567:Membranes, Artificial; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000378:metabolism*; D051381:Rats
chemical_list: D008567:Membranes, Artificial; D044925:Oxidoreductases Acting on CH-CH Group Donors; C019789:biliverdin reductase