title: Suppression of BSEP and MRP2 in mouse liver by miroestrol and deoxymiroestrol isolated from Pueraria candollei.
abstract: Miroestrol and deoxymiroestrol are highly active phytoestrogens isolated from the tuberous root of Pueraria candollei var. mirifica (Leguminosae). Modulatory effects of miroestrol and deoxymiroestrol on the mRNAs of BSEP and MRP2 genes involved in bile salt transportation, in C57BL/6 mice were investigated. In contrast to estradiol, miroestrol and deoxymiroestrol suppressed the expression of BSEP and MRP2 mRNA in both male and female mice. The results suggest for the first time that the use of miroestrol and deoxymiroestrol-containing products as alternative medicines or health supplements should be concerned according to their effects on key genes that regulate the bile salt export pump, which could result in the risk of hepatotoxicity and intrahepatic cholestasis.
authors.affiliation: Academic Office for Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Latiporn
authors.initials: L
authors.lastname: Udomsuk
authors.forename: Thaweesak
authors.initials: T
authors.lastname: Juengwatanatrakul
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Jarukamjorn
mesh_terms: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002780:Cholestasis, Intrahepatic / Q000139:chemically induced / Q000235:genetics; D003374:Coumarins / Q000009:adverse effects / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008107:Liver Diseases / Q000209:etiology / Q000235:genetics; D008297:Male; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D048789:Phytoestrogens / Q000009:adverse effects / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000009:adverse effects* / Q000494:pharmacology; D018517:Plant Roots; D035281:Plant Tubers; D029908:Pueraria / Q000737:chemistry*; D012333:RNA, Messenger / Q000378:metabolism; D013256:Steroids / Q000009:adverse effects* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000074020:ATP Binding Cassette Transporter, Subfamily B, Member 11; D018528:ATP-Binding Cassette Transporters; C490549:Abcb11 protein, mouse; D003374:Coumarins; D026901:Membrane Transport Proteins; D048789:Phytoestrogens; D010936:Plant Extracts; D012333:RNA, Messenger; D013256:Steroids; C406602:deoxymiroestrol; C571572:miroestrol