title: Gene-expression profiles and transcriptional regulatory pathways that underlie the identity and diversity of mouse tissue macrophages.
abstract: We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were not present in all macrophages. Nonetheless, well-characterized surface markers, including MerTK and FcγR1 (CD64), along with a cluster of previously unidentified transcripts, were distinctly and universally associated with mature tissue macrophages. TCEF3, C/EBP-α, Bach1 and CREG-1 were among the transcriptional regulators predicted to regulate these core macrophage-associated genes. The mRNA encoding other transcription factors, such as Gata6, was associated with single macrophage populations. We further identified how these transcripts and the proteins they encode facilitated distinguishing macrophages from dendritic cells.
authors.affiliation: Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine, New York, New York, USA.
authors.forename: Emmanuel L
authors.initials: EL
authors.lastname: Gautier
authors.forename: Tal
authors.initials: T
authors.lastname: Shay
authors.forename: Jennifer
authors.initials: J
authors.lastname: Miller
authors.forename: Melanie
authors.initials: M
authors.lastname: Greter
authors.forename: Claudia
authors.initials: C
authors.lastname: Jakubzick
authors.forename: Stoyan
authors.initials: S
authors.lastname: Ivanov
authors.forename: Julie
authors.initials: J
authors.lastname: Helft
authors.forename: Andrew
authors.initials: A
authors.lastname: Chow
authors.forename: Kutlu G
authors.initials: KG
authors.lastname: Elpek
authors.forename: Simon
authors.initials: S
authors.lastname: Gordonov
authors.forename: Amin R
authors.initials: AR
authors.lastname: Mazloom
authors.forename: Avi
authors.initials: A
authors.lastname: Ma'ayan
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Chua
authors.forename: Ted H
authors.initials: TH
authors.lastname: Hansen
authors.forename: Shannon J
authors.initials: SJ
authors.lastname: Turley
authors.forename: Miriam
authors.initials: M
authors.lastname: Merad
authors.forename: Gwendalyn J
authors.initials: GJ
authors.lastname: Randolph
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D002454:Cell Differentiation; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D014644:Genetic Variation; D008099:Liver / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics* / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: D015703:Antigens, CD; D012333:RNA, Messenger; D014157:Transcription Factors