title: Top-down platform for deciphering the human salivary proteome.
abstract: Proteomic platforms can be classified in bottom-up strategies, which analyze the sample after proteolytic digestion, and top-down strategies, which analyze the intact naturally occurring proteome. Bottom-up platforms are high-throughput because they can investigate a large number of proteins, regardless of their dimension. Nonetheless, information on post-translational modifications (PTMs) can be lost, especially those regarding naturally occurring cleavages and alternative splicing. Top-down platforms cannot cover vast proteomes, however, they can disclose subtle structural variations occurring during protein maturation and allow label-free relative quantifications in an unlimited number of samples. A repertoire of 256 masses belonging to naturally occurring proteins and peptides consistently detected by RP-HPLC-ESI-MS analysis of the acidic soluble fraction of human whole saliva is presented in this study. Of them, 233 have been identified, while 23 are still pending for the definitive characterization. The present review reports average and mono-isotopic masses of the peptides and proteins detected, RP-HPLC elution times, PTMs, origin and quali-quantitative variations observed in several physiological and pathological conditions. The information reported can be a reference for users of top-down RP-HPLC-ESI-MS proteomic platforms applied to the study of the human salivary proteome as well as of other human bodily fluids.
authors.affiliation: Istituto di Biochimica e Biochimica Clinica, Facoltà di Medicina, Università Cattolica, Largo Francesco Vito 1, Rome, Italy. massimo.castagnola@icrm.cnr.it
authors.forename: Massimo
authors.initials: M
authors.lastname: Castagnola
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cabras
authors.forename: Federica
authors.initials: F
authors.lastname: Iavarone
authors.forename: Federica
authors.initials: F
authors.lastname: Vincenzoni
authors.forename: Alberto
authors.initials: A
authors.lastname: Vitali
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Pisano
authors.forename: Sonia
authors.initials: S
authors.lastname: Nemolato
authors.forename: Emanuele
authors.initials: E
authors.lastname: Scarano
authors.forename: Antonella
authors.initials: A
authors.lastname: Fiorita
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vento
authors.forename: Chiara
authors.initials: C
authors.lastname: Tirone
authors.forename: Costantino
authors.initials: C
authors.lastname: Romagnoli
authors.forename: Massimo
authors.initials: M
authors.lastname: Cordaro
authors.forename: Gaetano
authors.initials: G
authors.lastname: Paludetti
authors.forename: Gavino
authors.initials: G
authors.lastname: Faa
authors.forename: Irene
authors.initials: I
authors.lastname: Messana
mesh_terms: D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D002648:Child; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism; D054808:Histatins / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012463:Saliva / Q000737:chemistry*; D055273:Salivary Proline-Rich Proteins / Q000032:analysis; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D054808:Histatins; D010446:Peptide Fragments; D020543:Proteome; D055273:Salivary Proline-Rich Proteins; D012471:Salivary Proteins and Peptides