title: Endogenous cytokinin and auxin profiles during in vitro organogenesis from vegetative buds of Pinus radiata adult trees.
abstract: In Pinus radiata D. Don, the transition from the juvenile to the mature phase is characterized by a reduction in the tree's organogenic potential, which is usually reverted in breeding programs by reinvigoration procedures to enable vegetative propagation. In this work, we have determined the best culture conditions for in vitro reinvigoration of radiata pine buds, tested different cytokinin (CK) types [N⁶-benzyladenine (BA), meta-topolin (mT) and trans-zeatin] and concentrations (25 and 50 µM), and studied the effect of culture conditions on endogenous CK and indole-3-acetic acid (IAA) levels at different stages of the organogenic process. To this end, the levels of 43 CKs and IAA were determined in P. radiata buds before and during the reinvigoration process. When BA or mT was applied to the induction medium, we did not observe any significant increase or decrease in endogenous isoprenoid CK content. We also report for the first time the presence of O-glucosides in non-treated P. radiata explants from the field and remark the importance of O-glucosides as storage forms.
authors.affiliation: Neiker-Tecnalia, Departamento de Biotecnología, Centro de Arkaute, E-01080, Vitoria-Gazteiz, Spain.
authors.forename: Itziar Aurora
authors.initials: IA
authors.lastname: Montalbán
authors.forename: Ondrej
authors.initials: O
authors.lastname: Novák
authors.forename: Jakub
authors.initials: J
authors.lastname: Rolčik
authors.forename: Miroslav
authors.initials: M
authors.lastname: Strnad
authors.forename: Paloma
authors.initials: P
authors.lastname: Moncaleán
mesh_terms: D001593:Benzyl Compounds; D002853:Chromatography, Liquid; D003583:Cytokinins / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D005960:Glucosides / Q000032:analysis / Q000378:metabolism; D007210:Indoleacetic Acids / Q000032:analysis / Q000378:metabolism*; D007701:Kinetin / Q000494:pharmacology; D013058:Mass Spectrometry; D028223:Pinus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011687:Purines; D013729:Terpenes / Q000032:analysis / Q000378:metabolism; D014197:Trees; D015026:Zeatin / Q000494:pharmacology
chemical_list: D001593:Benzyl Compounds; D003583:Cytokinins; D005960:Glucosides; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D013729:Terpenes; C030737:indoleacetic acid; D015026:Zeatin; C480551:benzylaminopurine; D007701:Kinetin