title: The effect of recombinant stromal cell-derived factor-1 treatment on hypoxic-ischemic brain injury in neonatal mice.
abstract: BACKGROUND
  Stromal cell-derived factor-1 (SDF-1) plays a critical role in adult brain injury repair including neurogenesis and vasculogenesis. However, the effects of recombinant SDF-1(rSDF-1) treatment on hypoxic-ischemic (HI) brain injury in neonatal mice are not clear.
  
  
  METHODS
  Seven-day-old (P7) C57BL/6J mice were divided into sham group, control group, and rSDF-1 group. Mice brains were collected to determine histopathological damage and the expression level of SDF-1, caspase-3, and Ki67 on P8, P10, P14, and P21. Passive avoidance and elevated plus-maze tests were also assessed on P20 and P21.
  
  
  RESULTS
  Compared with control group, rSDF-1 treatment increased the weight ratio of left and right brain hemisphere (p < 0.05), reduced the number of electric foot shocks (p < 0.05) and the percentage of time spent in the open arms (p < 0.05), meanwhile, increased the retention latency (p < 0.05) and the percentage of time spent in the enclosed arms (p < 0.05) on P20 and P21. High expression of Ki67 and low expression of caspase-3 were also observed.
  
  
  CONCLUSIONS
  This study showed that rSDF-1 treatment effectively alleviated brain injury shown by reducing in caspase-3 expression and increasing in Ki67 expression. Moreover, rSDF-1 treatment significantly improved behavioral performances in juvenile mice after HI.
authors.affiliation: Department of Pediatrics, Jinan Central Hospital affiliated to Shandong University, Jinan, China.
authors.forename: WeiWei
authors.initials: W
authors.lastname: Zhu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ma
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Lichun
authors.initials: L
authors.lastname: Yu
authors.forename: Mi
authors.initials: M
authors.lastname: Xu
authors.forename: Aihua
authors.initials: A
authors.lastname: Ma
authors.forename: Aili
authors.initials: A
authors.lastname: Guo
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001362:Avoidance Learning / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D054377:Chemokine CXCL12 / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D020925:Hypoxia-Ischemia, Brain / Q000188:drug therapy* / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; D019394:Ki-67 Antigen; D011994:Recombinant Proteins; D053148:Caspase 3