title: CD44+/CD105+ human amniotic fluid mesenchymal stem cells survive and proliferate in the ovary long-term in a mouse model of chemotherapy-induced premature ovarian failure.
abstract: OBJECTIVE
  Stem cell transplantation has been reported to rescue ovarian function in a preclinical mouse model of chemotherapy-induced premature ovarian failure (POF); however, maintaining the survival and self-renewal of transplanted seed cells in ovarian tissues over the long-term remains a troublesome issue. In this study we aimed to determine whether the CD44+/CD105+ human amniotic fluid cell (HuAFCs) subpopulation represent potential seed cells for stem cell transplantation treatments in POF.
  
  
  METHODS
  The CD44+/CD105+ subpopulation were isolated from HuAFCs, cultured in vitro, and injected into a cyclophosphamide-induced mouse model of POF.
  
  
  RESULTS
  Under continuous subculture in vitro, CD44+/CD105+ cells proliferated rapidly and expressed high levels of the proliferative markers Ki67 and survivin, as well as high levels of a number of mesenchymal stem cell biomarkers. Moreover, when red fluorescence protein (RFP)-transduced CD44+/CD105+ HuAFCs were transplanted into the ovaries of POF mice, the cells could be detected by fluorescence microscopy up to three weeks after injection. Furthermore, the BrdUrd incorporation assay and immunofluorescent staining demonstrated that CD44+/CD105+ HuAFCs underwent normal cycles of cell proliferation and self-renewal in the ovarian tissues of POF mice over the long-term.
  
  
  CONCLUSIONS
  The mesenchymal stem cell properties and long-term in vivo survival of CD44+/CD105+ HuAFCs make them ideal seed cells for stem cell transplantation to treat POF.
authors.affiliation: Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200031, China. liute79@yahoo.com
authors.forename: Te
authors.initials: T
authors.lastname: Liu
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Huang
authors.forename: Lihe
authors.initials: L
authors.lastname: Guo
authors.forename: Weiwei
authors.initials: W
authors.lastname: Cheng
authors.forename: Gang
authors.initials: G
authors.lastname: Zou
mesh_terms: D000653:Amniotic Fluid / Q000276:immunology*; D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D000970:Antineoplastic Agents / Q000009:adverse effects*; D001483:Base Sequence; D002469:Cell Separation; D003520:Cyclophosphamide / Q000009:adverse effects*; D017931:DNA Primers; D004195:Disease Models, Animal; D000071063:Endoglin; D005260:Female; D018960:Hyaluronan Receptors / Q000276:immunology*; D059630:Mesenchymal Stem Cells / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016649:Primary Ovarian Insufficiency / Q000139:chemically induced* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D000970:Antineoplastic Agents; D017931:DNA Primers; C064206:ENG protein, human; D000071063:Endoglin; D018960:Hyaluronan Receptors; D011956:Receptors, Cell Surface; D003520:Cyclophosphamide