title: Production of ligninolytic enzymes by solid-state fermentation using Pleurotus eryngii.
abstract: Pleurotus eryngii (DC.) Gillet (MCC58) was investigated for its ability to produce various ligninolytic enzymes such as laccase (Lac), manganese peroxidase (MnP), aryl alcohol oxidase (AAO), and lignin peroxidase (LiP) by solid-state fermentation (SSF), which was carried out using a support substrate from the fruit juice industry. The chemical content of grape waste from this industry was studied. Also, the production patterns of these extracellular enzymes were researched during the growth of the organism for a period of 20 days and the protein, reducing sugar, and nitrogen levels were monitored during the stationary cultivation. The highest Lac activity was obtained as 2247.62 ± 75 U/L on day 10 in the presence of 750 µM Mn²⁺, while the highest MnP activity was attained as 2198.44 ± 65 U/L on day 15 in the presence of 500 µM Mn²⁺. Decolorization of methyl orange and reactive red 2 azo dyes was also achieved with ligninolytic enzymes, produced in SSF of P. eryngii.
authors.affiliation: Chemistry Department, Graduate School of Natural and Applied Sciences, Dokuz Eylul University, Izmir, Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Akpinar
authors.forename: Raziye Ozturk
authors.initials: RO
authors.lastname: Urek
mesh_terms: D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000737:chemistry; D004789:Enzyme Activation; D005285:Fermentation; D005638:Fruit / Q000737:chemistry; D005656:Fungal Proteins / Q000096:biosynthesis* / Q000737:chemistry; D007218:Industrial Microbiology / Q000379:methods; D007220:Industrial Waste / Q000517:prevention & control; D042845:Laccase / Q000096:biosynthesis* / Q000737:chemistry; D008031:Lignin / Q000737:chemistry; D009282:Naphthalenesulfonates / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D010544:Peroxidases / Q000096:biosynthesis* / Q000737:chemistry; D020076:Pleurotus / Q000737:chemistry / Q000201:enzymology*; D014227:Triazines / Q000737:chemistry; D027843:Vitis / Q000737:chemistry
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D005656:Fungal Proteins; D007220:Industrial Waste; D009282:Naphthalenesulfonates; D014227:Triazines; C098610:reactive red 2; C100258:methyl orange; D008031:Lignin; D000429:Alcohol Oxidoreductases; C077759:aryl-alcohol oxidase; D042845:Laccase; D010544:Peroxidases; C042858:lignin peroxidase; C051129:manganese peroxidase; D009584:Nitrogen