title: Identification of isoquercitrin metabolites produced by human intestinal bacteria using UPLC-Q-TOF/MS.
abstract: In this paper, ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and the MetaboLynx™ software combined with mass defect filtering were applied to identity the metabolites of isoquercitrin using an intestinal mixture of bacteria and 96 isolated strains from human feces. The human incubated samples collected for 72 h in the anaerobic incubator and extracted with ethyl acetate were analyzed by UPLC-Q-TOF/MS within 10 min. The parent compound and five metabolites were identified by eight isolated strains, including Bacillus sp. 17, Veillonella sp. 23 and 32 and Bacteroides sp. 40, 41, 56, 75 and 88 in vitro. The results indicate that quercetin, acetylated isoquercitrin, dehydroxylated isoquercitrin, hydroxylated quercetin and hydroxymethylated quercetin are the major metabolites of isoquercitrin. Furthermore, a possible metabolic pathway for the biotransformation of isoquercitrin was established in intestinal flora. This study will be helpful for understanding the metabolic route of isoquercitrin and the role of different intestinal bacteria in the metabolism of natural compounds.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, People's Republic of China.
authors.forename: Linling
authors.initials: L
authors.lastname: Lu
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Shu
authors.initials: S
authors.lastname: Jiang
authors.forename: Jianming
authors.initials: J
authors.lastname: Guo
authors.forename: Er-xin
authors.initials: EX
authors.lastname: Shang
authors.forename: Jin-ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D013058:Mass Spectrometry / Q000379:methods; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism
chemical_list: C016527:isoquercitrin; D011794:Quercetin