title: The cellular uptake and cytotoxic effect of curcuminoids on breast cancer cells.
abstract: OBJECTIVE
  Curcuminoids (including curcumin) are natural antioxidants demonstrating potent chemopreventive properties against several forms of cancer. This study investigated the antiproliferative and induced apoptotic effects of curcuminoids on three cell lines isolated from human breast adenocarcinoma and ductal carcinoma (MDA-MB-231, MDA-MB-435S, and MCF-7).
  
  
  METHODS
  This study developed a highly sensitive, reproducible assay method using high-pressure liquid chromatography to quantify the cellular uptake of curcuminoids by breast cancer cells and quantitate its effect on inhibition of proliferation and activation of apoptosis in breast cancer cells.
  
  
  RESULTS
  Results indicate that curcuminoids inhibited cell proliferation and activation of apoptosis in the cell lines in this study. Both effects were observed to increase in proportion to the cellular uptake of curcuminoids; cellular uptake increased following an increase in the dosage of curcuminoids.
  
  
  CONCLUSIONS
  The inhibition of proliferation and increased apoptosis of breast cancer cells appears to be associated with the uptake of curcuminoids by cancer cells.
authors.affiliation: Department of Obstetrics and Gynecology, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Chang
authors.forename: Chi-Feng
authors.initials: CF
authors.lastname: Fu
authors.forename: Wei-Te
authors.initials: WT
authors.lastname: Yang
authors.forename: Tzu-Yu
authors.initials: TY
authors.lastname: Chen
authors.forename: Yi-Chiang
authors.initials: YC
authors.lastname: Hsu
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001682:Biological Availability; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D018270:Carcinoma, Ductal, Breast / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D003474:Curcumin / Q000031:analogs & derivatives / Q000493:pharmacokinetics / Q000494:pharmacology*; D036381:Diarylheptanoids; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans
chemical_list: D000970:Antineoplastic Agents; D036381:Diarylheptanoids; C034786:bisdemethoxycurcumin; D003474:Curcumin; C050229:demethoxycurcumin