title: HIV-1 antisense transcription is preferentially activated in primary monocyte-derived cells.
abstract: In this study, an antisense luciferase-expressing human immunodeficiency virus type 1 (HIV-1) molecular clone was used to infect primary cells. We found that antisense transcription activity from the 3' long terminal repeat (LTR) was significantly more abundant in monocyte-derived cells than in activated T lymphocytes. Moreover, by analyzing antisense transcription in infected monocyte-derived dendritic cells (MDDCs), we observed that the majority of HIV-1-infected MDDCs with significant antisense transcription activity did not produce Gag. We also confirmed that the negative-strand-encoded antisense protein (ASP) was expressed in monocyte-derived cells.
authors.affiliation: Université Montpellier 1, Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé (CPBS), Montpellier, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Laverdure
authors.forename: Antoine
authors.initials: A
authors.lastname: Gross
authors.forename: Charlotte
authors.initials: C
authors.lastname: Arpin-André
authors.forename: Isabelle
authors.initials: I
authors.lastname: Clerc
authors.forename: Bruno
authors.initials: B
authors.lastname: Beaumelle
authors.forename: Benoit
authors.initials: B
authors.lastname: Barbeau
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mesnard
mesh_terms: D002478:Cells, Cultured; D005814:Genes, Viral; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D009000:Monocytes / Q000821:virology*; D016372:RNA, Antisense / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D016372:RNA, Antisense