title: [Study on absorption ingredients of yuanhuzhitong pill by everted intestinal sac method].
abstract: OBJECTIVE
  To establish an everted intestinal sac method for determining absorption ingredients of yuanhuzhitong pill and study absorption characteristics of major chemical constituents of yuanhuzhitong pill.
  
  
  METHODS
  The everted intestinal sac model was adopted. Intestinal sac fluid samples at different time points after administration of three concentrations of Yuanhuzhitong pill were collected and determined by HPLC. The accumulative absorbed doses of active constituents were calculated, while the proportion between samples of yuanhuzhitong pill and absorption ingredients was compared.
  
  
  RESULTS
  Eight ingredients of Yuanhuzhitong pill can be detected in intestinal sac, they are protopine, palmatine, coptisine, imperatorin, berberine, byakangelicin, alpha-allocryptopine and tetrahydropalmatine. The absorption rate constants (Ka) of eight constituents increased in jejunum and ileum with the increase in concentration of Yuanhuzhitong pill extracts (P < 0. 05), indicating a passive absorption.
  
  
  CONCLUSIONS
  As ingredients are selectively absorbed in intestinal sac, the everted intestinal sac method is selected to assess the intestinal absorption characteristics of ingredients of Yuanhuzhitong prescription.
authors.affiliation: College of life science and engineering, Southwest Jiaotong University, Chengdu 610031, China. juying169@yahoo.com.cn
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Chen
authors.forename: Yingchun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shuo
authors.initials: S
authors.lastname: Lin
authors.forename: Hongjun
authors.initials: H
authors.lastname: Yang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wu
authors.forename: Shaojing
authors.initials: S
authors.lastname: Li
authors.forename: Defeng
authors.initials: D
authors.lastname: Li
authors.forename: Haiyu
authors.initials: H
authors.lastname: Xu
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031311:Corydalis / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000493:pharmacokinetics*; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008516:Medicine, Chinese Traditional; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal