title: Mitochondrial DNA as effective molecular markers for the genetic variation and phylogeny of the family Osteoglossidae.
abstract: The present study examined the genetic variation of the family Osteoglossidae from different geographical locations based on the mitochondrial NADH dehydrogenase subunit 2 (ND2) and ATPase subunit 6 (ATPase6) genes; we then re-constructed the phylogenetic relationships using the two sequences in combination. The results showed that the partial sequences of mitochondrial ND2 and ATPase6 of the family Osteoglossidae were 813 bp and 669 bp, respectively. A total of 42 species-specific nucleotide positions of the family Osteoglossidae were found to be useful for molecular identification. The sequence variation showed greater differences (8.3%~28.1% for the combined sequences, 8.3%~26.7% for the ND2 gene, and 9.3%~28.7% for the ATPase6 gene) among the different species of Osteoglossidae, and there was a significant association between the genetic difference and geographical location. Phylogenetic analyses using neighbor-joining, Bayesian inference, and maximum parsimony (MP) methods based on the combined sequences of the two genes were able to distinguish the different species and were in agreement with the existing taxonomy based on morphological characters and in association with the geographical distribution among seven species of the family Osteoglossidae.
authors.affiliation: Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture, Guangzhou 510380, China.
authors.forename: Xi-dong
authors.initials: XD
authors.lastname: Mu
authors.forename: Xue-jie
authors.initials: XJ
authors.lastname: Wang
authors.forename: Hong-mei
authors.initials: HM
authors.lastname: Song
authors.forename: Ye-xin
authors.initials: YX
authors.lastname: Yang
authors.forename: Du
authors.initials: D
authors.lastname: Luo
authors.forename: Dang-en
authors.initials: DE
authors.lastname: Gu
authors.forename: Meng
authors.initials: M
authors.lastname: Xu
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Jian-ren
authors.initials: JR
authors.lastname: Luo
authors.forename: Yin-chang
authors.initials: YC
authors.lastname: Hu
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D005399:Fishes / Q000235:genetics*; D014644:Genetic Variation; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D009245:NADH Dehydrogenase; D000251:Adenosine Triphosphatases