title: Circulating microparticles in severe preeclampsia.
abstract: The present study aimed to evaluate microparticles (MPs) from different sources in women with severe preeclampsia (PE) compared with normotensive pregnant women and non-pregnant women. This case-control study evaluated 28 pregnant women with severe PE, 30 normotensive pregnant women, and 29 non-pregnant women. MPs from neutrophils, endothelial cells, monocytes, platelets, leukocytes, erythrocytes, and syncytiotrophoblast were evaluated using flow cytometry. A higher total number of MPs were observed in women with severe PE compared with normotensive pregnant women and non-pregnant women (P=0.004 and P=0.001, respectively). MPs derived from erythrocytes were increased in women with severe PE compared with normotensive pregnant women (P=0.002). A trend towards association was observed between platelet count and the number of MPs derived from platelets (P=0.09) in severe PE group. A positive correlation was also found between the number of endothelial cell-derived MPs and the number of platelet-derived MPs, leukocyte-derived MPs, neutrophil-derived MPs, and lymphocyte-derived MPs (P<0.05) in severe PE pregnant women. MP counts can be increased in severe PE, and erythrocyte and endothelial cell-derived MPs seem to be associated to severe PE.
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Fabiana K
authors.initials: FK
authors.lastname: Marques
authors.forename: Fernanda M F
authors.initials: FM
authors.lastname: Campos
authors.forename: Olindo A M
authors.initials: OA
authors.lastname: Filho
authors.forename: Andrea T
authors.initials: AT
authors.lastname: Carvalho
authors.forename: Luci M S
authors.initials: LM
authors.lastname: Dusse
authors.forename: Karina B
authors.initials: KB
authors.lastname: Gomes
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D055252:Cell-Derived Microparticles; D042783:Endothelial Cells / Q000166:cytology; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D010976:Platelet Count; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D012720:Severity of Illness Index; D012984:Software; D055815:Young Adult