title: Association of three polymorphisms of scavenger receptor class BI gene (exon8, exon1, intron5) with coronary stenosis in a coronary Tunisian population.
abstract: BACKGROUND
  The potential role of scavenger receptor class BI (gene name SCARB1) in the regulation of lipoproteins metabolism and atherosclerosis has attracted considerable interest. We tested the relationship of SCARB1 polymorphisms with significant coronary stenosis (SCS) and lipid profile in a coronary Tunisian population.
  
  
  METHODS
  Three SCARB1 polymorphisms (exon8 (C/T), exon1 (G/A), intron5 (C/T)) were studied in 316 Tunisian patients undergoing coronary angiography. SCS was defined as a luminal narrowing of ≥ 50% in at least one major coronary artery. Lipid profile was measured. Genotyping was performed using PCR-RFLP.
  
  
  RESULTS
  Individuals with TT genotypes of exon8 were associated with higher concentrations of plasma HDL-C and ApoAI in the group without SCS. Carriers of T allele of exon8 were associated with 41% lower risk of SCS. This protective effect seemed to be particularly significant in women, nondiabetics and nonsmokers. Subjects homozygous for the variant allele of intron5 were significantly associated with an increased risk of SCS, particularly in smokers. AA genotype of exon1 was associated with an increased risk of SCS in diabetics and in patients with metabolic syndrome. The (CAT) haplotype was associated with increase in the risk of SCS compared to the wild haplotype and had a 4-fold greater risk of SCS than patients with haplotype (TGC) which seems to be the most protective against SCS.
  
  
  CONCLUSIONS
  Carriers of T allele of exon8 in SCARB1 seemed to increase HDL-C and ApoAI concentrations and reduce the risk of SCS. The intron5, exon1 and (CAT) haplotype seemed to have an atherogenic effect.
authors.affiliation: Biochemistry Department, UR MSP 28/04, Sahloul University Hospital, Sousse, Tunisia.
authors.forename: Jihène
authors.initials: J
authors.lastname: Rejeb
authors.forename: Asma
authors.initials: A
authors.lastname: Omezzine
authors.forename: Imen
authors.initials: I
authors.lastname: Boumaiza
authors.forename: Lamia
authors.initials: L
authors.lastname: Rebhi
authors.forename: Slim
authors.initials: S
authors.lastname: Kacem
authors.forename: Nabila Ben
authors.initials: NB
authors.lastname: Rejeb
authors.forename: Naoufel
authors.initials: N
authors.lastname: Nabli
authors.forename: Ahmed Ben
authors.initials: AB
authors.lastname: Abdelaziz
authors.forename: Essia
authors.initials: E
authors.lastname: Boughzala
authors.forename: Ali
authors.initials: A
authors.lastname: Bouslama
mesh_terms: D000368:Aged; D023921:Coronary Stenosis / Q000235:genetics*; D005091:Exons; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006579:Heterozygote; D006801:Humans; D007438:Introns; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D051122:Scavenger Receptors, Class B / Q000235:genetics*; D014416:Tunisia
chemical_list: C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B