title: Structural and immunological studies of hetero polysaccharide isolated from the alkaline extract of Tricholoma crassum (Berk.) Sacc.
abstract: An immunoenhancing water-soluble hetero polysaccharide was isolated from an alkaline extract of the fruit bodies of an ectomycorrhizal edible mushroom, Tricholoma crassum (Berk.) Sacc. The structure of the molecule was investigated using acid hydrolysis, methylation analysis; periodate oxidation study, and NMR spectroscopy techniques ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). On the basis of the above-mentioned experiments the structure of the repeating unit of the polysaccharide was established as: This polysaccharide exhibited splenocyte, thymocyte as well as macrophage activations.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India.
authors.forename: Pradip
authors.initials: P
authors.lastname: Patra
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Bhanja
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.forename: Surajit
authors.initials: S
authors.lastname: Samanta
authors.forename: Debsankar
authors.initials: D
authors.lastname: Das
authors.forename: K Sanjana P
authors.initials: KS
authors.lastname: Devi
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000468:Alkalies; D000818:Animals; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D045424:Complex Mixtures / Q000737:chemistry*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006868:Hydrolysis; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D051379:Mice; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000096:biosynthesis; D010084:Oxidation-Reduction; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D060168:Thymocytes / Q000187:drug effects / Q000378:metabolism; D055439:Tricholoma / Q000737:chemistry*
chemical_list: D000468:Alkalies; D045424:Complex Mixtures; D062610:Fungal Polysaccharides; D007155:Immunologic Factors; D009569:Nitric Oxide