title: Bidirectional frequency-dependent effect of extremely low-frequency electromagnetic field on E. coli K-12.
abstract: In the present work, the frequency-dependent effects of extremely low-frequency electromagnetic field (ELF EMF) on Escherichia coli K-12 growth have been studied. The frequency-dependent effects of ELF EMF have shown that it can either stimulate or inhibit the growth of microbes. However, the mechanism by which the ELF EMF affects the bacterial cells is not clear yet. It was suggested that the aqua medium can serve as a target through which the biological effect of ELF EMF on microbes may be realized. To check this hypothesis, the frequency-dependent effects (2, 4, 6, 8, 10 Hz, B = 0.4 mT, 30 min) of ELF EMF on the bacterial growth were studied in both cases where the microbes were in the culture media during the exposure and where culture media was preliminarily exposed to the ELF EMF before the addition of bacteria. For investigating the cell proliferation, the radioactive [(3)H]-thymidine assay was carried out. It has been shown that EMF at 4 Hz exposure has pronounced stimulation while at 8 Hz it has inhibited cell proliferation.
authors.affiliation: Department of Biotechnology, Life Sciences International Postgraduate Educational Center, Yerevan, Armenia. varsikmartirosyan@gmail.com
authors.forename: Varsik
authors.initials: V
authors.lastname: Martirosyan
authors.forename: Naira
authors.initials: N
authors.lastname: Baghdasaryan
authors.forename: Sinerik
authors.initials: S
authors.lastname: Ayrapetyan
mesh_terms: D049109:Cell Proliferation / Q000528:radiation effects; D004574:Electromagnetic Fields / Q000009:adverse effects*; D048168:Escherichia coli K12 / Q000166:cytology / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D013936:Thymidine / Q000378:metabolism
chemical_list: D013936:Thymidine