title: Bioinformatic analysis of α/β-hydrolase fold enzymes reveals subfamily-specific positions responsible for discrimination of amidase and lipase activities.
abstract: Superfamily of alpha-beta hydrolases is one of the largest groups of structurally related enzymes with diverse catalytic functions. Bioinformatic analysis was used to study how lipase and amidase catalytic activities are implemented into the same structural framework. Subfamily-specific positions--conserved within lipases and peptidases but different between them--that were supposed to be responsible for functional discrimination have been identified. Mutations at subfamily-specific positions were used to introduce amidase activity into Candida antarctica lipase B (CALB). Molecular modeling was implemented to evaluate influence of selected residues on binding and catalytic conversion of amide substrate by corresponding library of mutants. In silico screening was applied to select reactive enzyme-substrate complexes that satisfy knowledge-based criteria of amidase catalytic activity. Selected CALB variants with substitutions at subfamily-specific positions Gly39, Thr103, Trp104, and Leu278 were produced and showed significant improvement of experimentally measured amidase activity. Based on these results, we suggest that value of subfamily-specific positions should be further explored in order to develop a systematic tool to study structure-function relationship in enzymes and to use this information for rational enzyme engineering.
authors.affiliation: Faculty of Bioengineering and Bioinformatics and Belozersky Institute of Physicochemical Biology, Lomonosov Moscow State University, Lenin Hills 1/73, Moscow 119991, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Suplatov
authors.forename: W
authors.initials: W
authors.lastname: Besenmatter
authors.forename: V K
authors.initials: VK
authors.lastname: Svedas
authors.forename: A
authors.initials: A
authors.lastname: Svendsen
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry / Q000378:metabolism*; D019943:Amino Acid Substitution; D002175:Candida / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D020134:Catalytic Domain; D019295:Computational Biology / Q000379:methods*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006867:Hydrolases / Q000737:chemistry / Q000378:metabolism*; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation; D015202:Protein Engineering
chemical_list: D005656:Fungal Proteins; D006867:Hydrolases; D008049:Lipase; C431349:lipase B, Candida antarctica; D000581:Amidohydrolases; C019665:amidase