title: Sensitive and simple determination of bromate in foods disinfected with hypochlorite reagents using high performance liquid chromatography with post-column derivatization.
abstract: A novel analytical method for the quantification of bromate in fresh foods using high performance liquid chromatography (HPLC) with a post-column reaction has been developed. The fresh food sample solutions were pretreated with homogenization, centrifugal ultrafiltration and subsequent solid phase extraction using a strong anion-exchange resin. After separation on a strong anion-exchange chromatography column using a highly concentrated NaCl solution (0.3M) as the eluent, the bromate was quantified by detection using a post-column reaction with a non-carcinogenic reagent (tetramethylbenzidine). The developed HPLC technique made it possible to quantify bromate in salt-rich fresh foods. The recoveries from fresh foods spiked with bromate at low levels (2 or 10 ng/g) satisfactorily ranged from 75.3 to 90.7%. The lowest quantification limit in fresh foods was estimated to be 0.6 ng/g as bromic acid. The method should be helpful for the quantification of bromate in fresh foods disinfected with hypochlorite solutions.
authors.affiliation: Graduate School of Health Sciences, Gunma University, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
authors.forename: Azusa
authors.initials: A
authors.lastname: Yokota
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kubota
authors.forename: Satomi
authors.initials: S
authors.lastname: Komiya
authors.forename: Kyoko
authors.initials: K
authors.lastname: Sato
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.forename: Ichiro
authors.initials: I
authors.lastname: Koshiishi
mesh_terms: D000818:Animals; D001959:Bromates / Q000032:analysis*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004202:Disinfectants / Q000737:chemistry; D004203:Disinfection / Q000379:methods; D060766:Drinking Water / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D059022:Food Safety / Q000379:methods*; D006997:Hypochlorous Acid / Q000737:chemistry*; D008460:Meat / Q000032:analysis; D010473:Perciformes; D012965:Sodium Chloride / Q000737:chemistry; D052616:Solid Phase Extraction; D014462:Ultrafiltration; D014675:Vegetables / Q000737:chemistry
chemical_list: D001959:Bromates; D004202:Disinfectants; D060766:Drinking Water; D012965:Sodium Chloride; D006997:Hypochlorous Acid