title: The JAK2/STAT3 signal pathway regulates the expression of genes related to skeletal muscle development and energy metabolism in mice and mouse skeletal muscle cells.
abstract: To investigate the effects of the JAK2/STAT3 pathway on skeletal muscle development and energy metabolism, AG490 and IL-11 were used as agonist and inhibitor in treating mice and the mouse skeletal muscle cells. Average body weight (ABW) was reduced significantly in the mice treated with AG490 (p<0.05), while IL-11 had the opposite effect (p<0.05), as compared with the controls, average body temperature (ABT) remained at normal levels in both groups. Western blotting was used to determine the protein levels of JAK2, STAT3, p-JAK2, and p-STAT3. AG490 caused significant decreases in p-JAK2 and p-STAT3 (p<0.05), while IL-11 did the opposite (p<0.05, p<0.01). Quantitative RT-PCR also showed significantly decreased expression levels of Myf5, MyoD, LXRa, and UCP3 in the AG490 group (p<0.01), but in the IL-11 group, the expression of Myf5, MyoD, and UCP3 was increased (p<0.05), except that LXRa whose expression did not change. In cultured skeletal muscle cells, the expression of MyoD, Myf5, LXRa, and UCP3 (p<0.05) exhibited the same trend as that in the skeletal muscles of both treated groups (p<0.05). These results implicate the JAK2/STAT3 in skeletal muscle development and energy metabolism.
authors.affiliation: College of Animal Science and Technology, Northwest Agriculture and Forestry University, Yangling, Shaanxi, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Xubin
authors.initials: X
authors.lastname: Jing
authors.forename: Lu
authors.initials: L
authors.lastname: Gan
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
mesh_terms: D000818:Animals; D001823:Body Composition / Q000187:drug effects; D001831:Body Temperature / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D017370:Interleukin-11 / Q000494:pharmacology; D053614:Janus Kinase 2 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D020032:Tyrphostins / Q000494:pharmacology
chemical_list: D017370:Interleukin-11; D050796:STAT3 Transcription Factor; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D053614:Janus Kinase 2