title: Differential expression of G-protein-coupled estrogen receptor-30 in human myometrial and uterine leiomyoma smooth muscle.
abstract: OBJECTIVE
  To determine differential expression of G-protein-coupled receptor 30 (GPR30) in uterine leiomyoma and its matched myometrium.
  
  
  METHODS
  GPR30 expression examined in both tissues and cultured cells.
  
  
  METHODS
  Research laboratories.
  
  
  METHODS
  Women 35 to 50 years old with uterine leiomyomas.
  
  
  METHODS
  Hysterectomy.
  
  
  METHODS
  GPR30 expression profile.
  
  
  RESULTS
  Using Western blot and real-time quantitative polymerase chain reaction analyses, we found that GPR30 was highly expressed in uterine leiomyomas compared with their matched myometrium. In only three out of nine patients examined was GPR30 protein detectable by Western blot analysis in myometrial tissues, but at statistically significantly lower levels than in their leiomyomas. Confocal microscopy revealed the nuclear localization of GPR30 in leiomyoma tissues and cultured leiomyoma smooth muscle cells (SMCs). Treatment with 0.1 μM 17β-estradiol increased mRNA expression of GPR30 in leiomyoma SMCs but decreased expression in myometrial SMCs. Treatment with G-1, a GPR30 agonist, stimulated phosphorylation of p44/42 mitogen-activated protein kinase (MAPK) in both SMC types. PD98059, the MEK inhibitor, completely inhibited G-1-induced phosphorylation of p44/42 in myometrium SMCs, but not in SMCs from leiomyoma.
  
  
  CONCLUSIONS
  GPR30 is abundantly expressed in uterine leiomyomas, likely resulting from estrogen stimulation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Nankai University School of Medicine, Tianjin, People's Republic of China.
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Tian
authors.affiliation: College of Pharmacy, Nankai University, Tianjin, People's Republic of China.
authors.forename: Zengyong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Biochemistry and Molecular Biology, Nankai University School of Medicine, Tianjin, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Nankai University School of Medicine, Tianjin, People's Republic of China.
authors.forename: Yuebing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Gynecology, Tianjin Central Hospital for Obstetrics and Gynecology, Tianjin, People's Republic of China.
authors.forename: Yingjun
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Gynecology, Tianjin Central Hospital for Obstetrics and Gynecology, Tianjin, People's Republic of China.
authors.forename: Wanjun
authors.initials: W
authors.lastname: Lin
authors.affiliation: College of Pharmacy, Nankai University, Tianjin, People's Republic of China; Smooth Muscle Research Group, Department of Physiology and Pharmacology, Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, Alberta, Canada.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gui
authors.affiliation: Department of Biochemistry and Molecular Biology, Nankai University School of Medicine, Tianjin, People's Republic of China; Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, Alberta, Canada. Electronic address: xlzheng@ucalgary.ca.
authors.forename: Xi-Long
authors.initials: XL
authors.lastname: Zheng
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D007889:Leiomyoma / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D009215:Myometrium / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D011804:Quinolines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C517943:1-(4-(6-bromobenzo(1,3)dioxol-5-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta(c)quinolin-8-yl)ethanone; D003517:Cyclopentanes; D005419:Flavonoids; C109584:GPER1 protein, human; D011804:Quinolines; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D043562:Receptors, G-Protein-Coupled; D004958:Estradiol; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one