title: Time-resolved detection of fluorescent light during inflow of ICG to the brain-a methodological study.
abstract: It was reported that time-resolved reflectance measurements carried out during inflow and washout of an optical contrast agent may provide information on the blood supply to the brain cortex of human adults. It was also shown that a measurement of fluorescence excited in the dye circulating in the brain is feasible. Unfortunately, patterns of time-resolved fluorescence signals observed during in vivo measurements are difficult to interpret. The aim of this study was to analyze the influence of several factors on the fluorescence signals measured during in vivo experiments. A laboratory instrument for recording the distributions of arrival of fluorescence photons was constructed and optimized for measurements on humans. Monte Carlo simulations and laboratory measurements on liquid phantoms as well as in vivo measurements on healthy volunteers were carried out. An influence of source-detector separation, position of the source-detector pair on the head, as well as a dose of the injected indocyanine green (ICG) on the fluorescence signals were studied in detail. It was shown that even for a small dose of ICG (0.025 mg kg(-1)) the time-resolved signals can be successfully detected on the surface of the head. Strong influence of the studied factors on the fluorescence signals was observed. It was also noted that the changes in moments of distributions of arrival times of fluorescence photons depend on the anatomical structure of the tissues located between the source and the detector.
authors.affiliation: Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland. dmilej@ibib.waw.pl
authors.forename: Daniel
authors.initials: D
authors.lastname: Milej
authors.forename: Anna
authors.initials: A
authors.lastname: Gerega
authors.forename: Norbert
authors.initials: N
authors.lastname: Zołek
authors.forename: Wojciech
authors.initials: W
authors.lastname: Weigl
authors.forename: Michał
authors.initials: M
authors.lastname: Kacprzak
authors.forename: Piotr
authors.initials: P
authors.lastname: Sawosz
authors.forename: Joanna
authors.initials: J
authors.lastname: Mączewska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Fronczewska
authors.forename: Ewa
authors.initials: E
authors.lastname: Mayzner-Zawadzka
authors.forename: Leszek
authors.initials: L
authors.lastname: Królicki
authors.forename: Roman
authors.initials: R
authors.lastname: Maniewski
authors.forename: Adam
authors.initials: A
authors.lastname: Liebert
mesh_terms: D001692:Biological Transport; D001921:Brain / Q000378:metabolism*; D003287:Contrast Media / Q000378:metabolism*; D005453:Fluorescence; D006801:Humans; D007208:Indocyanine Green / Q000378:metabolism*; D007267:Injections; D009010:Monte Carlo Method; D019047:Phantoms, Imaging; D013997:Time Factors
chemical_list: D003287:Contrast Media; D007208:Indocyanine Green