title: [Comparative study on pseudoanaphylactoid reactions induced by medicinal tween 80 and injectable tween 80].
abstract: OBJECTIVE
  To investigate the safety of different level of tween 80 by comparing the degree of pseudoanaphylactoid reactions (PR) induced by medicinal tween 80 and injectable tween 80.
  
  
  METHODS
  The analysis of vascular permeability of the mice ears: ICR mouse were divided into different test groups, the mice were intravenously injected with solutions of medicinal tween 80 and injectable tween 80 with 0.2%, 1% and 5% concentration, positive control Compound 48/80 and 5% glucose injection. All test substances were mixed with 0.4% Evans blue. The reaction and vascular permeability of the ears were observed and measured 30 min after injection. The analysis of vascular permeability of the rat's skin: the rats were intravenous injected with 0. 6% Evans blue normal saline solution first, 10 minutes later, the same substances were intradermal administrated into the back of rats. The rats were sacrificed and the diameter of locus ceruleus and the content of Evans blue leak out were measured 20 min after injection.
  
  
  RESULTS
  Medicinal tween 80 and injectable tween 80 with 5% concentration caused obvious vascular hyper permeability in ICR mice, but the degree of vascular hyperpermeability caused by injectable tween 80 was lighter than by medicinal tween 80. Other tween 80 didn't cause obvious vascular hyper permeability in the ears of mouse. The solution of different concentration of tween 80 caused obvious locus ceruleus reaction in rat's back. As for the content Evans blue leak out, there was no statistical significance between each group except positive control Compound 48/80 group.
  
  
  CONCLUSIONS
  Tween 80 can cause obvious vascular hyper permeability and the effect is dose dependent, which indicated that tween 80 can cause PR. On the other hand, injectable tween 80 is more security than medicinal tween 80, the dosage of tween 80 should be still controlled strictly so that to decrease the incidence of PR.
authors.affiliation: Capital Medical University, School of Traditional Chinese Medicine, Beijing 100069, China. wangyunting0@sina.com
authors.forename: Yunting
authors.initials: Y
authors.lastname: Wang
authors.forename: Chunying
authors.initials: C
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Yi
authors.forename: Lin
authors.initials: L
authors.lastname: Qin
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Guiqing
authors.initials: G
authors.lastname: Li
authors.forename: Xudong
authors.initials: X
authors.lastname: Cong
authors.forename: Sumin
authors.initials: S
authors.lastname: Cao
authors.forename: Aihua
authors.initials: A
authors.lastname: Liang
mesh_terms: D000707:Anaphylaxis / Q000139:chemically induced*; D000818:Animals; D007267:Injections / Q000379:methods; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011136:Polysorbates / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011136:Polysorbates