title: Presence and stability of B complex vitamins in bee pollen using different storage conditions.
abstract: This study has the objective of evaluating the stability of B complex vitamins and its vitamers, for a period of 1year of storage. The pollen samples were stored under room temperature (with and without light) and frozen. The vitamins were quantified by HPLC with fluorescence detection. All proposed vitamins were found in the samples and the dehydration process did not interfere in vitamin content. The variations were (dry basis): 0.59-1.09mg/100g (B(1)); 1.73-2.56 (B(2)); 6.43-15.34 (PP) and 0.33-0.68 (B(6)). After 1year of storage, it can be stated that vitamin B(1) concentration remained constant, while for the others, the concentration loss was dependent on time rather than on storage conditions. All samples were considered vitamin B(2) sources. The influence of the storage time in the concentrations of vitamin B(6) and PP was explained mathematically, through linear regression equations of multivariate analysis.
authors.affiliation: Laboratory of Food Analysis, Department of Food and Experimental Nutrition, Pharmaceutical Science School, University of São Paulo, Av. Prof. Lineu Prestes, 580, Bloco 14, Cidade Universitária, CEP 05508-900, São Paulo, SP, Brazil.
authors.forename: Vanilda Aparecida Soares
authors.initials: VA
authors.lastname: de Arruda
authors.forename: Aline Aparecida
authors.initials: AA
authors.lastname: Santos Pereira
authors.forename: Leticia M
authors.initials: LM
authors.lastname: Estevinho
authors.forename: Ligia Bicudo
authors.initials: LB
authors.lastname: de Almeida-Muradian
mesh_terms: D000818:Animals; D001516:Bees; D002851:Chromatography, High Pressure Liquid; D061353:Food Storage / Q000379:methods*; D005615:Freezing; D015999:Multivariate Analysis; D011058:Pollen / Q000737:chemistry*; D012256:Riboflavin / Q000032:analysis; D013831:Thiamine / Q000032:analysis; D013997:Time Factors; D025101:Vitamin B 6 / Q000032:analysis; D014803:Vitamin B Complex / Q000032:analysis* / Q000737:chemistry*
chemical_list: D014803:Vitamin B Complex; D025101:Vitamin B 6; D012256:Riboflavin; D013831:Thiamine