title: Quantification of collagen fiber organization using three-dimensional Fourier transform-second-harmonic generation imaging.
abstract: We present three-dimensional Fourier transform-second-harmonic generation (3D FT-SHG) imaging, a generalization of the previously reported two-dimensional FT-SHG, to quantify collagen fiber organization from 3D image stacks of biological tissues. The current implementation calculates 3D preferred orientation of a region of interest, and classifies regions of interest based on orientation anisotropy and average voxel intensity. Presented are some example applications of the technique which reveal the layered structure of collagen fibers in porcine sclera, and estimates the cut angle of porcine tendon tissues. This technique shows promising potential for studying biological tissues that contain fibrillar structures in 3D.
authors.affiliation: Photonics Research of Bio/nano Environments (PROBE), Department of Mechanical Science and Engineering, University of Illinois Urbana-Champaign, 1206 W Green St, Urbana, Illinois 61801, USA.
authors.forename: Tung Yuen
authors.initials: TY
authors.lastname: Lau
authors.forename: Raghu
authors.initials: R
authors.lastname: Ambekar
authors.forename: Kimani C
authors.initials: KC
authors.lastname: Toussaint
mesh_terms: D000818:Animals; D024022:Fibrillar Collagens / Q000737:chemistry*; D005583:Fourier Analysis; D021621:Imaging, Three-Dimensional / Q000379:methods*; D012590:Sclera / Q000033:anatomy & histology; D034421:Sus scrofa; D013710:Tendons / Q000033:anatomy & histology
chemical_list: D024022:Fibrillar Collagens