title: Modulation of Golgi-associated microtubule nucleation throughout the cell cycle.
abstract: A microtubule (MT) subpopulation that emanates from Golgi membrane has been recently shown to comprise a significant part of MT network in interphase cells. In this study, we address whether Golgi membrane, which is being extensively remodeled throughout the cell cycle, retains its ability to nucleate MTs at diverse cell cycle stages. Live cell imaging and immunofluorescence microscopy reveals that Golgi-derived MTs form at multiple stages of the cell cycle, including G(1), G(2), and distinct phases of mitosis. However, the capacity of Golgi to nucleate MTs in mitosis is strongly down-regulated as compared with interphase, indicating that this property is cell cycle regulated. We demonstrate that Golgi-derived MTs are indispensable for efficient Golgi assembly in telophase, and speculate that these noncentrosomal MTs may hold specific functions at other cell cycle stages.
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, 465 21st Avenue South, Nashville, TN 37232, USA.
authors.forename: Ana Rita Ramada
authors.initials: AR
authors.lastname: Maia
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhu
authors.forename: Paul
authors.initials: P
authors.lastname: Miller
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Gu
authors.forename: Helder
authors.initials: H
authors.lastname: Maiato
authors.forename: Irina
authors.initials: I
authors.lastname: Kaverina
mesh_terms: D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D005434:Flow Cytometry; D016193:G1 Phase / Q000235:genetics / Q000502:physiology; D016195:G2 Phase / Q000235:genetics / Q000502:physiology; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis / Q000235:genetics / Q000502:physiology