title: Efficient production of L-asparaginase from Bacillus licheniformis with low-glutaminase activity: optimization, scale up and acrylamide degradation studies.
abstract: L-Asparaginase has potential as an anti-cancer drug and for prevention of acrylamide formation in fried and baked foods. Production of the enzyme by Bacillus licheniformis (RAM-8) was optimized by process engineering using a statistical modeling approach and a maximum yield of 32.26 IU/ml was achieved. The L-asparaginase exhibited glutaminase activity of only 0.8 IU/ml and would therefore be less prone to cause the side effects associated with asparaginase therapy compared to enzyme preparations with higher glutaminase activities. When production was carried out in a 30-L bioreactor, enzyme production reached 29.94 IU/ml in 15 h. The enzyme inhibited poly-acrylamide formation in 10% acrylamide solution and reduced acrylamide formation in fried potatoes by 80%.
authors.affiliation: Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
authors.forename: Richi V
authors.initials: RV
authors.lastname: Mahajan
authors.forename: Saurabh
authors.initials: S
authors.lastname: Saran
authors.forename: Karthikeya
authors.initials: K
authors.lastname: Kameswaran
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.forename: R K
authors.initials: RK
authors.lastname: Saxena
mesh_terms: D001215:Asparaginase / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification*; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D003198:Computer Simulation; D004789:Enzyme Activation; D005972:Glutaminase / Q000096:biosynthesis* / Q000737:chemistry; D008954:Models, Biological
chemical_list: D001215:Asparaginase; D005972:Glutaminase