title: Microsatellite markers for Amborella (Amborellaceae), a monotypic genus endemic to New Caledonia.
abstract: OBJECTIVE
  Informative markers are required for assessing the diversity of Amborella trichopoda, the only species of its order, endemic to New Caledonia and considered to be the sister species to all flowering plants. Therefore, expressed sequence tag (EST)-based microsatellite markers were developed. •
  
  
  RESULTS
  Fifty-five microsatellite loci were characterized in 14896 putative unigenes, which were generated by assembling A. trichopoda ESTs from the public sequence database. Seventeen markers revealed polymorphism in 80 adult shrubs from three populations. The number of alleles per locus ranged from two to 12, with a total of 132 alleles scored. The mean expected heterozygosity per population ranged from 0.336 to 0.567. •
  
  
  CONCLUSIONS
  These markers offer an appropriate amount of variation to investigate genetic diversity structure, gene flow, and other conservation issues.
authors.affiliation: Institut de Recherche pour le Développement (IRD), Unité Mixte de Recherche-Diversité Adaptation et Développement des plantes (UMR DIADE), BP 64501, 34394 Montpellier Cedex 5, France. alerie.poncet@ird.fr
authors.forename: Valérie
authors.initials: V
authors.lastname: Poncet
authors.forename: Marie
authors.initials: M
authors.lastname: Couderc
authors.forename: Christine
authors.initials: C
authors.lastname: Tranchant-Dubreuil
authors.forename: Céline
authors.initials: C
authors.lastname: Gomez
authors.forename: Perla
authors.initials: P
authors.lastname: Hamon
authors.forename: Serge
authors.initials: S
authors.lastname: Hamon
authors.forename: Yohan
authors.initials: Y
authors.lastname: Pillon
authors.forename: Jérôme
authors.initials: J
authors.lastname: Munzinger
authors.forename: Alexandre
authors.initials: A
authors.lastname: de Kochko
mesh_terms: D018744:DNA, Plant / Q000235:genetics / Q000302:isolation & purification; D056426:Genetic Loci / Q000235:genetics; D019684:Magnoliopsida / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D009510:New Caledonia; D011110:Polymorphism, Genetic
chemical_list: D018744:DNA, Plant