title: Therapeutic effect of transplanting magnetically labeled bone marrow stromal stem cells in a liver injury rat model with 70%-hepatectomy.
abstract: BACKGROUND
  There are only few reports about the use of bone marrow stromal stem cells (BMSCs) for the treatment of traumatic liver injury. This study aimed to study the therapeutic effect of fluorescence-labeled BMSCs administered to rats subject to traumatic liver injury.
  
  
  METHODS
  Male SD rats with a 70% resection of the liver were injected with feridex-labeled BMSCs which could be induced to functional hepatocytes in vitro. Liver function was assayed and the liver scanned by 1.5-T MRI at 12 hrs and on days 1, 3, 5, 7, and 14 post-operation. The pathological changes of liver sections were monitored.
  
  
  RESULTS
  The serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, direct bilirubin, and total bilirubin in the transplantation group were significantly lower than the control group. The MRI showed rats of the transplantation group had an oval low signal area at 12 hr after operation; the low signal range gradually expanded and the signal intensity gradually decreased over 14 days after operation. The low signal range in the control group disappeared 12 hr after the operation. After Prussian blue staining, rats of the transplantation group contained blue granules with no significant hypertrophy or edema in hepatocytes, while the control group showed no blue granules with significant hypertrophy and edema.
  
  
  CONCLUSIONS
  The BMSCs transplanted into the injured rat liver gradually migrate to the surrounding liver tissue and partially repair the liver surgical injury in rats. BMSCs may represent an effective therapeutic approach for acute liver injury.
authors.affiliation: First People's Hospital of Shunde, Southern Medical University, Shunde, Guangdong, China.
authors.forename: Xiao-Wu
authors.initials: XW
authors.lastname: Chen
authors.forename: Da-Jian
authors.initials: DJ
authors.lastname: Zhu
authors.forename: Yong-Le
authors.initials: YL
authors.lastname: Ju
authors.forename: Shu-Feng
authors.initials: SF
authors.lastname: Zhou
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D048430:Cell Shape; D003911:Dextrans / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D006498:Hepatectomy; D022781:Hepatocytes / Q000473:pathology; D008099:Liver / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D008107:Liver Diseases / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D008111:Liver Function Tests; D060328:Magnetic Phenomena; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D013194:Staining and Labeling
chemical_list: D015415:Biomarkers; D003911:Dextrans; D058185:Magnetite Nanoparticles; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; C065507:ferumoxides; D001663:Bilirubin