title: Isolation and gene expression analysis of a papain-type cysteine protease in thermogenic skunk cabbage (Symplocarpus renifolius).
abstract: Skunk cabbage (Symplocarpus renifolius) spadices contain abundant transcripts for cysteine protease (CP). From thermogenic spadices, we isolated SrCPA, a highly expressed CP gene that encoded a papain-type CP. SrCPA is structurally similar to other plant CPs, including the senescence-associated CPs found in aroids. The expression of SrCPA increased during floral development, and was observed in all floral tissues except for the stamens.
authors.affiliation: Organization for Promotion of Tenure Track, University of Miyazaki, Miyazaki, Japan. ykoina@cc.miyazaki-u.ac.jp
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Ito-Inaba
authors.forename: Hiromi
authors.initials: H
authors.lastname: Masuko
authors.forename: Masao
authors.initials: M
authors.lastname: Watanabe
authors.forename: Takehito
authors.initials: T
authors.lastname: Inaba
mesh_terms: D000595:Amino Acid Sequence; D029064:Araceae / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D010206:Papain / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D010206:Papain