title: Effects of fullerene derivatives on bioluminescence and application for protease detection.
abstract: The effects of two C(60) derivatives: C(60)[C(COOH)(2)](3) and C(60)(NH(2))(x)(OH)(y), have been investigated on the bioluminescence of humanized Gaussia luciferase (hGluc) for the first time. Utilizing the dual properties of carboxyl C(60) with luminescence quenching and free radicals scavenging, a novel BRET system was constructed for protease detection with high sensitivity.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, P. R. China.
authors.forename: Junping
authors.initials: J
authors.lastname: Yu
authors.forename: Mirong
authors.initials: M
authors.lastname: Guan
authors.forename: Fengyun
authors.initials: F
authors.lastname: Li
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Chunru
authors.initials: C
authors.lastname: Wang
authors.forename: Chunying
authors.initials: C
authors.lastname: Shu
authors.forename: Hongping
authors.initials: H
authors.lastname: Wei
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhang
mesh_terms: D037741:Fullerenes / Q000737:chemistry* / Q000494:pharmacology; D057230:Limit of Detection; D008156:Luciferases / Q000032:analysis* / Q000187:drug effects; D049449:Luminescence; D015394:Molecular Structure; D010447:Peptide Hydrolases / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D037741:Fullerenes; D008156:Luciferases; D010447:Peptide Hydrolases