title: [Extracellular beta-D-glucosidase of the Penicillium canescens marine fungus].
abstract: Extracellular beta-D-glucosidase was isolated in a homogeneous state from the Penicillium canescens marine fungus. According to SDS-electrophoresis, the molecular weight of the enzyme was 64 kDa and the maximal activity was observed at pH 5.2 and 70 degrees C. Glucosidase catalyzed the hydrolysis of beta-glycosidic bonds both in glycosides and in glucose disaccharides and had transglycosylation activity. The enzyme can be used for the deglycosylation of natural glycosides and in enzymatic synthesis of new carbon-containing compounds.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Dubrovskaia
authors.forename: V V
authors.initials: VV
authors.lastname: Sova
authors.forename: N N
authors.initials: NN
authors.lastname: Slinkina
authors.forename: S D
authors.initials: SD
authors.lastname: Anastiuk
authors.forename: M V
authors.initials: MV
authors.lastname: Pivkin
authors.forename: T N
authors.initials: TN
authors.lastname: Zviagintseva
mesh_terms: D059001:Aquatic Organisms; D004187:Disaccharides / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000378:metabolism; D006027:Glycosides / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008970:Molecular Weight; D010407:Penicillium / Q000737:chemistry / Q000201:enzymology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D004187:Disaccharides; D005656:Fungal Proteins; D006027:Glycosides; D001617:beta-Glucosidase