title: Effects of elevated CO₂ and temperature on photosynthesis and leaf traits of an understory dwarf bamboo in subalpine forest zone, China.
abstract: The dwarf bamboo (Fargesia rufa Yi), growing understory in subalpine dark coniferous forest, is one of the main foods for giant panda, and it influences the regeneration of subalpine coniferous forests in southwestern China. To investigate the effects of elevated CO₂, temperature and their combination, the dwarf bamboo plantlets were exposed to two CO₂ regimes (ambient and double ambient CO₂ concentration) and two temperatures (ambient and +2.2°C) in growth chambers. Gas exchange, leaf traits and carbohydrates concentration were measured after the 150-day experiment. Elevated CO₂ significantly increased the net photosynthetic rate (Anet ), intrinsic water-use efficiency (WUEi ) and carbon isotope composition (δ¹³C) and decreased stomatal conductance (g(s)) and total chlorophyll concentration based on mass (Chl(m)) and area (Chl(a)). On the other hand, elevated CO₂ decreased specific leaf area (SLA), which was increased by elevated temperature. Elevated CO₂ also increased foliar carbon concentration based on mass (C(m)) and area (C(a)), nitrogen concentration based on area (N(a)), carbohydrates concentration (i.e. sucrose, sugar, starch and non-structural carbohydrates) and the slope of the A(net)-N(a) relationship. However, elevated temperature decreased C(m), C(a) and N(a). The combination of elevated CO₂ and temperature hardly affected SLA, C(m), C(a), N(m), N(a), Chl(m) and Chl(a). Variables Anet and Na had positive linear relationships in all treatments. Our results showed that photosynthetic acclimation did not occur in dwarf bamboo at elevated CO₂ and it could adjust physiology and morphology to enable the capture of more light, to increase WUE and improve nutritional conditions.
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Li
authors.forename: Yuanbin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Zhang
authors.forename: Helena
authors.initials: H
authors.lastname: Korpelainen
authors.forename: Frank
authors.initials: F
authors.lastname: Berninger
authors.forename: Chunyang
authors.initials: C
authors.lastname: Li
mesh_terms: D031723:Bambusa / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002244:Carbon / Q000032:analysis / Q000378:metabolism; D002245:Carbon Dioxide / Q000494:pharmacology* / Q000502:physiology; D002247:Carbon Isotopes / Q000032:analysis; D002681:China; D002734:Chlorophyll / Q000378:metabolism; D008027:Light; D058503:Mesophyll Cells; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D010641:Phenotype; D010788:Photosynthesis / Q000187:drug effects*; D018515:Plant Leaves / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure; D018526:Plant Transpiration / Q000187:drug effects; D036226:Seedlings / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure; D013696:Temperature; D014197:Trees; D014867:Water / Q000502:physiology
chemical_list: D002247:Carbon Isotopes; D014867:Water; D002734:Chlorophyll; D002245:Carbon Dioxide; D002244:Carbon; D009584:Nitrogen