title: Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle.
abstract: Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon-guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early-differentiated satellite cells up-regulate a secreted neural chemorepellent Sema3A upon in vivo muscle-crush injury. The study also revealed that Sema3A expression is up-regulated in primary satellite-cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)-β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time-course of extracellular HGF, FGF2 and TGF-β3 concentrations after crush-injury of Gastrocnemius muscle in the rat lower hind-limb, using a combination of a non-homogenization/non-spin extraction of extracellular wound fluids and enhanced chemiluminescence-Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2-8 days post-crush, whereas active TGF-β3 increases after 12 days, providing a better understanding of the time-coordinated levels of HGF, FGF2 and TGF-β3 that drive regulation of Sema3A expression during regenerative intramuscular moto-neuritogenesis.
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School of Agriculture.
authors.forename: Mai-Khoi Q
authors.initials: MK
authors.lastname: DO
authors.forename: Takahiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Borjigin
authors.initials: B
authors.lastname: Gerelt
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sato
authors.forename: Wataru
authors.initials: W
authors.lastname: Mizunoya
authors.forename: Mako
authors.initials: M
authors.lastname: Nakamura
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Ikeuchi
authors.forename: Judy E
authors.initials: JE
authors.lastname: Anderson
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Tatsumi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D005346:Fibroblast Growth Factors / Q000032:analysis*; D017228:Hepatocyte Growth Factor / Q000032:analysis*; D018482:Muscle, Skeletal / Q000293:injuries*; D051381:Rats; D040121:Semaphorin-3A / Q000378:metabolism; D013997:Time Factors; D053782:Transforming Growth Factor beta3 / Q000032:analysis*
chemical_list: D040121:Semaphorin-3A; D053782:Transforming Growth Factor beta3; D005346:Fibroblast Growth Factors; D017228:Hepatocyte Growth Factor