title: Measurement of orientation and susceptibility ratios using a polarization-resolved second-harmonic generation holographic microscope.
abstract: Three-dimensional second-harmonic fields, sample orientation, and susceptibility ratios of biological samples are measured using polarization-resolved second-harmonic generation (SHG) microscopy. The three-dimensional (3D) polarization is gathered by measurement of a series of holograms for which excitation and analyzer polarizations are systematically varied, and the 3D SHG field is recovered through numerical back propagation. Harmonophore orientation is resolved in 3D from a sub-set of polarization-resolved SHG holograms. We further expand on previous approaches for the determination of susceptibility ratios, adding the calculation of multiple ratio values to allow intrinsic verification.
authors.affiliation: Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, Colorado,  USA.
authors.forename: David G
authors.initials: DG
authors.lastname: Winters
authors.forename: David R
authors.initials: DR
authors.lastname: Smith
authors.forename: Philip
authors.initials: P
authors.lastname: Schlup
authors.forename: Randy A
authors.initials: RA
authors.lastname: Bartels