title: Position-dependent correlations between DNA methylation and the evolutionary rates of mammalian coding exons.
abstract: DNA cytosine methylation is a central epigenetic marker that is usually mutagenic and may increase the level of sequence divergence. However, methylated genes have been reported to evolve more slowly than unmethylated genes. Hence, there is a controversy on whether DNA methylation is correlated with increased or decreased protein evolutionary rates. We hypothesize that this controversy has resulted from the differential correlations between DNA methylation and the evolutionary rates of coding exons in different genic positions. To test this hypothesis, we compare human-mouse and human-macaque exonic evolutionary rates against experimentally determined single-base resolution DNA methylation data derived from multiple human cell types. We show that DNA methylation is significantly related to within-gene variations in evolutionary rates. First, DNA methylation level is more strongly correlated with C-to-T mutations at CpG dinucleotides in the first coding exons than in the internal and last exons, although it is positively correlated with the synonymous substitution rate in all exon positions. Second, for the first exons, DNA methylation level is negatively correlated with exonic expression level, but positively correlated with both nonsynonymous substitution rate and the sample specificity of DNA methylation level. For the internal and last exons, however, we observe the opposite correlations. Our results imply that DNA methylation level is differentially correlated with the biological (and evolutionary) features of coding exons in different genic positions. The first exons appear more prone to the mutagenic effects, whereas the other exons are more influenced by the regulatory effects of DNA methylation.
authors.affiliation: Physical and Computational Genomics Division, Genomics Research Center, Academia Sinica, Taipei 115, Taiwan. trees@gate.sinica.edu.tw
authors.forename: Trees-Juen
authors.initials: TJ
authors.lastname: Chuang
authors.forename: Feng-Chi
authors.initials: FC
authors.lastname: Chen
authors.forename: Yen-Zho
authors.initials: YZ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D019175:DNA Methylation / Q000502:physiology*; D019143:Evolution, Molecular; D005091:Exons / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D008251:Macaca; D051379:Mice; D020125:Mutation, Missense; D016366:Open Reading Frames / Q000502:physiology*; D013045:Species Specificity