title: OsMIOX, a myo-inositol oxygenase gene, improves drought tolerance through scavenging of reactive oxygen species in rice (Oryza sativa L.).
abstract: Myo-inositol oxygenase (MIOX), a unique monooxygenase, catalyzes the oxidation of myo-inositol to d-glucuronic acid. However, the protective role of MIOX in plants against oxidative stress or drought stress remains unknown. In this study, the functional characterization of MIOX obtained from the cDNA library of upland rice (Oryza sativa L. cv. IRAT109), was performed. OsMIOX was expressed predominantly in the roots and induced by drought, H₂O₂, salt, cold and abscisic acid. The transgenic rice lines overexpressing OsMIOX showed obviously improved growth performance in the medium containing 200 mM mannitol. Further, the survival rate of leaves from the transgenic rice lines was significantly higher than that of the wild type plants under polyethylene glycol treatment. It was discovered that the activity of ROS-scavenging enzymes and proline content, as well as the transcript levels of many ROS scavenging genes were significantly increased in transgenic plants compared to the wild type plants under drought stress conditions. Together, these data suggest that OsMIOX has a specific function in drought stress tolerance by decreasing oxidative damage.
authors.affiliation: Key Lab of Crop Heterosis and Utilization of Ministry of Education, Beijing Key Lab of Crop Genetic Improvement, China Agricultural University, Beijing 100193, PR China.
authors.forename: Junzhi
authors.initials: J
authors.lastname: Duan
authors.forename: Minghui
authors.initials: M
authors.lastname: Zhang
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zhang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xiong
authors.forename: Pengli
authors.initials: P
authors.lastname: Liu
authors.forename: Jauhar
authors.initials: J
authors.lastname: Ali
authors.forename: Jinjie
authors.initials: J
authors.lastname: Li
authors.forename: Zichao
authors.initials: Z
authors.lastname: Li
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D002374:Catalase / Q000378:metabolism; D003080:Cold Temperature; D055864:Droughts; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D050562:Inositol Oxygenase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D016877:Oxidants / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011392:Proline / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors; D014867:Water / Q000378:metabolism / Q000494:pharmacology
chemical_list: D016877:Oxidants; D010937:Plant Growth Regulators; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D014867:Water; D012965:Sodium Chloride; D000040:Abscisic Acid; D011392:Proline; D006861:Hydrogen Peroxide; D002374:Catalase; D009195:Peroxidase; D050562:Inositol Oxygenase