title: One-step purification method for pyridylamino glycans.
abstract: Pyridylamino derivatization is suitable for the microanalysis of glycans but there is a problem in that by-products of the labeling reaction and fluorescent substances from samples occasionally interfere with the detection of pyridylamino glycans. We have reported a three-step purification method (S. Natsuka et al., FEBS J., 278, 452-460 (2011)). That method gives high purity and high yield for various glycans, but it is rather complicated. In this study I checked the efficiency of a one-step method with a spin column for the purification of pyridylamino glycans and found that it was excellent in respect of throughput. High-throughput analysis of N-glycans is desirable in glycomics.
authors.affiliation: Department of Biology, Faculty of Science, Niigata University, Niigata, Japan. natsuka@bio.sc.niigata-u.ac.jp
authors.forename: Shunji
authors.initials: S
authors.lastname: Natsuka
mesh_terms: D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D060748:Fetuins / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D011725:Pyridines / Q000737:chemistry*
chemical_list: D060748:Fetuins; D011134:Polysaccharides; D011725:Pyridines; C023666:pyridine