title: Evaluation of lipid damage related to pathological and physiological conditions.
abstract: Several diseases and xenobiotics are known to generate reactive species that may trigger oxidative stress when not properly scavenged by the antioxidant defenses and result in tissue damage. We investigated lipid peroxidation (LPO) as a possible mechanism for tissue damage in some pathologies, in the normal aging process, and in subjects exposed to organic solvents. Plasmatic malondialdehyde (MDA) was quantified by high-performance liquid chromatography with visible wavelength detection in 239 subjects and divided into the following: acute myocardium infarction (AMI), diabetes without complications (D) and hemodialysis (HD) patients; into healthy children, adults, and elderly, all nonexposed to xenobiotics; and into painters occupationally exposed to organic solvents (P). Troponin, glycated hemoglobin, and transminases [aspartate aminotransferase (AST) and alanine aminotransferase] were analyzed. An increase in LPO was observed in AMI, D, HD, and P groups, when compared to healthy adults. No correlation between MDA and age was found. Further, we found positive correlations between MDA versus troponin (r = 0.47), MDA versus HbA1c (r = 0.56), and MDA versus AST (r = 0.41) in AMI, diabetics, and painters, respectively. This work has demonstrated increased lipid and protein damages in myocardium and blood, along with an alteration of hepatic transaminase activities and induction of LPO, suggesting that MDA levels are important to evaluate the extent of tissue alterations and development of acute and chronic conditions.
authors.affiliation: Toxicology Laboratory, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Solange Cristina
authors.initials: SC
authors.lastname: Garcia
authors.forename: Denise
authors.initials: D
authors.lastname: Grotto
authors.forename: Rachel Picada
authors.initials: RP
authors.lastname: Bulcão
authors.forename: Angela Maria
authors.initials: AM
authors.lastname: Moro
authors.forename: Miguel
authors.initials: M
authors.lastname: Roehrs
authors.forename: Juliana
authors.initials: J
authors.lastname: Valentini
authors.forename: Fernando Araújo
authors.initials: FA
authors.lastname: de Freitas
authors.forename: Clovis
authors.initials: C
authors.lastname: Paniz
authors.forename: Guilherme Borges
authors.initials: GB
authors.lastname: Bubols
authors.forename: Mariele Feiffer
authors.initials: MF
authors.lastname: Charão
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000378:metabolism* / Q000473:pathology; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008111:Liver Function Tests; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D016273:Occupational Exposure / Q000009:adverse effects; D018384:Oxidative Stress / Q000502:physiology*; D010150:Paint / Q000009:adverse effects; D006435:Renal Dialysis / Q000009:adverse effects*; D012997:Solvents / Q000009:adverse effects; D000637:Transaminases / Q000097:blood; D014336:Troponin / Q000097:blood; D055815:Young Adult
chemical_list: D006442:Glycated Hemoglobin; D008315:Malondialdehyde; D012997:Solvents; D000637:Transaminases; D014336:Troponin