title: Measurement of glial fibrillary acidic protein.
abstract: Toxicant-induced injury of the adult or developing central nervous system results in hypertrophy of astrocytes at the site of injury. The hallmark of this response is enhanced expression of the major intermediate filament protein of astrocytes, glial fibrillary acid protein (GFAP). A protocol is provided for assaying GFAP levels in detergent homogenates of brain tissue using a sandwich ELISA.
authors.affiliation: Center for Disease Control and Prevention NIOSH, Morgantown, WV, USA.
authors.forename: James P
authors.initials: JP
authors.lastname: O'Callaghan
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis* / Q000378:metabolism; D007202:Indicators and Reagents; D012015:Reference Standards
chemical_list: D005904:Glial Fibrillary Acidic Protein; D007202:Indicators and Reagents