title: An important role of prostanoid receptor EP2 in host resistance to Mycobacterium tuberculosis infection in mice.
abstract: Mycobacterium tuberculosis, the causative agent of tuberculosis, resides and replicates within susceptible hosts by inhibiting host antimicrobial mechanisms. Prostaglandin E(2) (PGE(2)), produced by M. tuberculosis-infected macrophages, exerts a variety of immunomodulatory functions via 4 receptors (EP1-EP4), each mediating distinct PGE(2) functions. Here, we show that M. tuberculosis infection selectively upregulates EP2 messenger RNA expression in CD4(+) T cells. We found that EP2 deficiency in mice increases susceptibility to M. tuberculosis infection, which correlated with reduced antigen-specific T-cell responses and increased levels of CD4(+)CD25(+)Foxp3(+) T-regulatory cells. These findings have revealed an important role for EP2 in host immune defense against tuberculosis. As a G protein-coupled receptor, EP2 could serve as a target for immunotherapy of tuberculosis.
authors.affiliation: Immunology Group,  International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi,  India.
authors.forename: Vandana
authors.initials: V
authors.lastname: Kaul
authors.forename: Debapriya
authors.initials: D
authors.lastname: Bhattacharya
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Singh
authors.forename: Luc
authors.initials: L
authors.lastname: Van Kaer
authors.forename: Marc
authors.initials: M
authors.lastname: Peters-Golden
authors.forename: William R
authors.initials: WR
authors.lastname: Bishai
authors.forename: Gobardhan
authors.initials: G
authors.lastname: Das
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000032:analysis; D015870:Gene Expression; D020869:Gene Expression Profiling; D016130:Immunophenotyping; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D058307:Receptors, Prostaglandin E, EP2 Subtype / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000737:chemistry / Q000276:immunology; D014376:Tuberculosis / Q000235:genetics* / Q000276:immunology*
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; C508018:Il2ra protein, mouse; D053645:Interleukin-2 Receptor alpha Subunit; D058307:Receptors, Prostaglandin E, EP2 Subtype