title: Histone modification-mediated Lhx2 gene expression.
abstract: Lhx2, a member of LIM homeobox transcription factors, plays a key role in central nervous system (CNS) and embryonic tissue development. However, molecular mechanism of Lhx2 gene regulation remains largely unknown. Here, we identified and characterized a regulatory region of Lhx2 gene which mediates responses to two different signals such as inhibition of HDAC3 and stimulation by E2F1. In particular, the promoter region of -229 to -126 was responsible not only for basal expression but also for a inhibitor of histone deacetylase, trichostatin A (TSA)-mediated activation of Lhx2 gene. Intriguingly, transcription factor E2F1 also activates Lhx2 gene via direct binding to the same -229 to -126 region. Based on these observations, we could have demonstrated that E2F1 is necessary for TSA-mediated activation of Lhx2 gene and acetylation of histone 3 is involved in this event. This study provides evidence that the histone modification and E2F1 binding are integral parts of the mechanism for Lhx2 gene expression.
authors.affiliation: Department of Biochemistry, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Key Sun
authors.initials: KS
authors.lastname: Park
authors.forename: Kee Kwang
authors.initials: KK
authors.lastname: Kim
authors.forename: Kyoon Eon
authors.initials: KE
authors.lastname: Kim
mesh_terms: D000107:Acetylation; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D050687:E2F1 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D056572:Histone Deacetylase Inhibitors / Q000378:metabolism / Q000494:pharmacology; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D047468:Immunoprecipitation; D060850:LIM-Homeodomain Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation; D000099186:Histone Deacetylase 3
chemical_list: D050687:E2F1 Transcription Factor; D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases; D006657:Histones; D006877:Hydroxamic Acids; D060850:LIM-Homeodomain Proteins; D014157:Transcription Factors; D000099186:Histone Deacetylase 3; C493754:E2f1 protein, mouse; C489784:Lhx2 protein, mouse; C012589:trichostatin A