title: Effects of buyang huanwu decoction on ventricular remodeling and differential protein profile in a rat model of myocardial infarction.
abstract: Buyang Huanwu decoction (BYHWD) is a well-known and canonical Chinese medicine formula from "Correction on Errors in Medical Classics" in Qing dynasty. Here, we show that BYHWD could alleviate the ventricular remodeling induced by left anterior descending (LAD) artery ligation in rats. BYHWD treatment (18 g/kg/day) decreased heart weight/body weight (HW/BW), left ventricle (LV) dimension at end diastole (LVDd) and increased LV ejection fraction (LVEF) and LV fractional shortening (LVFS) significantly compared to model group at the end of 12 weeks. The collagen volume of BYHWD group was more significantly decreased than that of model group. Proteomic analysis showed that atrial natriuretic factor (ANF) was downregulated; heat shock protein beta-6 (HSPB6) and peroxiredoxin-6 (PRDX6) were upregulated in BYHWD-treated group among successfully identified proteins. The apoptotic index (AI) was reduced by BYHWD accompanied by decreased expression of Bax and caspase 3 activity, increased Bcl-2/Bax ratio, and phosphorylation of HSPB6 compared to that of model group. Taken together, these results suggest that BYHWD can alleviate ventricular remodeling induced by LAD artery ligation. The antiremodeling effects of BYHWD are conferred by decreasing AI through affecting multiple targets including increased Bcl-2/Bax ratio and decreased caspase 3 activity that might be via upregulated PRDX6, phosphorylation of HSPB6 and subsequently reduction of ANF.
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangdong, Guangzhou 510515, China ; The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Ying Chun
authors.initials: YC
authors.lastname: Zhou
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Ying Jia
authors.initials: YJ
authors.lastname: Li
authors.forename: Lin Lin
authors.initials: LL
authors.lastname: Jing
authors.forename: Ge
authors.initials: G
authors.lastname: Wen
authors.forename: Jing
authors.initials: J
authors.lastname: Tang
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Zhi Ping
authors.initials: ZP
authors.lastname: Lv
authors.forename: Xue Gang
authors.initials: XG
authors.lastname: Sun