title: Lactobacillus casei and Lactobacillus acidophilus regulate inflammatory pathway and improve antioxidant status in collagen-induced arthritic rats.
abstract: In view of well-established immunomodulatory properties of Lactobacillus, present investigation was carried out to evaluate antioxidant and anti-inflammatory potential of Lactobacillus casei and Lactobacillus acidophilus, against inflammatory pathway and oxidative stress developed in an experimental model of arthritis. Collagen-induced arthritis (CIA) model was used. Oral administration of L. casei, L. acidophilus, standard antiarthritic drug indomethacin, and vehicle were started after induced arthritis and continued up to day 28. Interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-1β, IL-17, IL-4, and IL-10 levels were estimated in serum. In parallel, oxidative stress parameters were also measured from synovial effsuate. All rats were graded for arthritis score at the end of each week. L. casei, L. acidophilus, and indomethacin treatment significantly downregulated proinflammatory and upregulated anti-inflammatory cytokines at P<0.0001. They have significantly decreased oxidative stress in synovial effsuate (P<0.0001) and also arthritis score (P<0.05). Protection provided by L. casei and L. acidophilus was more pronounced than that of indomethacin. These lines of evidence suggest that L. casei and L. acidophilus exert potent protective effect against CIA. It further establishes effective anti-inflammatory and antioxidant properties of Lactobacillus. However, additional clinical investigations are needed to prove the efficacy of Lactobacillus in treatment/management of rheumatoid arthritis.
authors.affiliation: Department of Microbiology, Barkatullah University, Bhopal, Madhya Pradesh, India.
authors.forename: Sarika
authors.initials: S
authors.lastname: Amdekar
authors.forename: Vinod
authors.initials: V
authors.lastname: Singh
authors.forename: Avnish
authors.initials: A
authors.lastname: Kumar
authors.forename: Poonam
authors.initials: P
authors.lastname: Sharma
authors.forename: Rambir
authors.initials: R
authors.lastname: Singh
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001169:Arthritis, Experimental / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D002374:Catalase / Q000276:immunology / Q000378:metabolism; D005978:Glutathione / Q000276:immunology / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000276:immunology / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000276:immunology; D007249:Inflammation / Q000276:immunology* / Q000382:microbiology; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology; D020381:Interleukin-17 / Q000097:blood / Q000276:immunology; D053583:Interleukin-1beta / Q000097:blood / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood / Q000276:immunology; D007779:Lactobacillus acidophilus / Q000276:immunology* / Q000502:physiology; D007780:Lacticaseibacillus casei / Q000276:immunology* / Q000502:physiology; D015227:Lipid Peroxidation / Q000187:drug effects / Q000276:immunology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000276:immunology; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D013482:Superoxide Dismutase / Q000276:immunology / Q000378:metabolism; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology
chemical_list: D000975:Antioxidants; D020381:Interleukin-17; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D015847:Interleukin-4; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione