title: Genistein and dicarboximide fungicides in infant formulae from the EU market.
abstract: A method based on ultrasonic extraction and purification by solid phase extraction followed by LC-MS/MS and GC-MS analysis was developed for the determination of genistein, genistin, iprodione, vinclozolin and procymidone in infant powdered formulas. The method was tested for different formulations: milk, soy and hypoallergenic, and was applied to European pooled samples. Spike recoveries ranged from 53.1% to 91.5% and the relative standard deviation values for repeatability ranged from 9.6% to 17.7%, except for iprodione in milk formula (22.3%). None of the fungicides were found in the European pooled formulae, while genistein was found at 9.7μg/g in soy formula and the concentration of genistin, its β-glycosylated form, was respectively 31.4ng/g and 476ng/g in milk and soy formula.
authors.affiliation: Mario Negri Institute for Pharmacological Research, Department of Environmental Health Sciences, Milan, Italy. silvia.maggioni@marionegri.it
authors.forename: Silvia
authors.initials: S
authors.lastname: Maggioni
authors.forename: Renzo
authors.initials: R
authors.lastname: Bagnati
authors.forename: Marchela
authors.initials: M
authors.lastname: Pandelova
authors.forename: Karl-Werner
authors.initials: KW
authors.lastname: Schramm
authors.forename: Emilio
authors.initials: E
authors.lastname: Benfenati
mesh_terms: D000818:Animals; D002417:Cattle; D005060:Europe; D005506:Food Contamination / Q000032:analysis* / Q000191:economics; D005659:Fungicides, Industrial / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D019833:Genistein / Q000032:analysis*; D041943:Infant Formula / Q000737:chemistry* / Q000191:economics; D008892:Milk / Q000737:chemistry; D052616:Solid Phase Extraction; D045730:Soy Foods / Q000032:analysis; D053719:Tandem Mass Spectrometry
chemical_list: D005659:Fungicides, Industrial; D019833:Genistein