title: MAIGO2 is involved in abscisic acid-mediated response to abiotic stresses and Golgi-to-ER retrograde transport.
abstract: The central role of multisubunit tethering complexes in intracellular trafficking has been established in yeast and mammalian systems. However, little is known about their roles in the stress responses and the early secretory pathway in Arabidopsis. In this study, Maigo2 (MAG2), which is equivalent to the yeast Tip20p and mammalian Rad50-interacting protein, is found to be required for the responses to salt stress, osmotic stress and abscisic acid in seed germination and vegetative growth, and MAG2-like (MAG2L) is partially redundant with MAG2 in response to environmental stresses. MAG2 strongly interacts with the central region of ZW10, and both proteins are important as plant endoplasmic reticulum (ER)-stress regulators. ER morphology and vacuolar protein trafficking are unaffected in the mag2, mag2l and zw10 mutants, and the secretory marker to the apoplast is correctly transported in mag2 plants, which indicate that MAG2 functions as a complex with ZW10, and is potentially involved in Golgi-to-ER retrograde trafficking. Therefore, a new role for ER-Golgi membrane trafficking in abiotic-stress and ER-stress responses is discovered.
authors.affiliation: Laboratory of Plant Stress Ecophysiology and Biotechnology, Shapotou Desert Research and Experiment Station, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China.
authors.forename: Pengshan
authors.initials: P
authors.lastname: Zhao
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Jiao
authors.initials: J
authors.lastname: Gong
authors.forename: Guanzhong
authors.initials: G
authors.lastname: Yu
authors.forename: Miao
authors.initials: M
authors.lastname: Ma
authors.forename: Yujian
authors.initials: Y
authors.lastname: Lu
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Jia
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000502:physiology*; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D018525:Germination; D006056:Golgi Apparatus / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins; D058503:Mesophyll Cells; D016254:Mutagenesis, Insertional; D009997:Osmotic Pressure; D021381:Protein Transport; D011523:Protoplasts; D012492:Salts; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D012639:Seeds / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015398:Signal Transduction; D013312:Stress, Physiological; D020798:Two-Hybrid System Techniques; D014617:Vacuoles / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C584748:MAG2L protein, Arabidopsis; C518500:Maigo2 protein, Arabidopsis; D012492:Salts; D000040:Abscisic Acid