title: Extension of chronological lifespan by ScEcl1 depends on mitochondria in Saccharomyces cerevisiae.
abstract: Ecl1, a product of the YGR146C gene in Saccharomyces cerevisiae, was identified as a factor involved in chronological lifespan. In this study we found evidence that the function of Ecl1 in the extension of chronological lifespan is dependent on mitochondrial function. The respiratory activity of cells increased when Ecl1 was overexpressed or cells were grown under calorie restriction, but there was no additive effect of calorie restriction and Ecl1 overexpression on increases in respiratory activity or on the extension of chronological lifespan. Based on these results, we propose that overexpression of Ecl1 has same effect as caloric restriction and that its function also depends on mitochondria, just like caloric restriction.
authors.affiliation: Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.
authors.forename: Kenko
authors.initials: K
authors.lastname: Azuma
authors.forename: Hokuto
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murakami
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Aiba
mesh_terms: D000818:Animals; D019069:Cell Respiration; D008928:Mitochondria / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: C547504:Ecl1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D010100:Oxygen