title: Vba5p, a novel plasma membrane protein involved in amino acid uptake and drug sensitivity in Saccharomyces cerevisiae.
abstract: Vba5p is closest to Vba3p in the vacuolar transporter for basic amino acids (VBA) family of Saccharomyces cerevisiae. We found that green fluorescence protein (GFP)-tagged Vba5p localized exclusively to the plasma membrane. The uptake of lysine and arginine by whole cells was little affected by deletion of the VBA5 gene, but was stimulated by overexpression of the VBA5 gene. The inhibitory effect of 4-nitroquinoline N-oxide on cell growth was accelerated by expression of the VBA5 gene, and was lessened by the addition of arginine. These results suggest that Vba5p is a plasma membrane protein involved in amino acid uptake and drug sensitivity.
authors.affiliation: Division of Applied Sciences, Graduate School of Engineering, Muroran Institute of Technology, Muroran, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Shimazu
authors.forename: Teruhiro
authors.initials: T
authors.lastname: Itaya
authors.forename: Pongsanat
authors.initials: P
authors.lastname: Pongcharoen
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sekito
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kawano-Kawada
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Kakinuma
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000595:Amino Acid Sequence; D026923:Amino Acid Transport Systems, Basic / Q000737:chemistry / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D026923:Amino Acid Transport Systems, Basic; D000596:Amino Acids; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C579334:Vba5 protein, S cerevisiae; D015112:4-Nitroquinoline-1-oxide