title: Measurement of expression of the HSP70 protein family.
abstract: The heat shock protein family of proteins include a number of proteins of similar sizes that are inducible by a wide variety of "stress" stimuli including heat shock, ischemia, toxic metals, amino acid analogs, and some chemotherapeutic and anesthetic chemicals. The functions of these proteins are diverse but are generally associated with molecular chaperoning of proteins. This unit contains a wide variety of protocols for analyzing expression of this family at the transcriptional and translational level: northern blot hybridization, in situ hybridization, metabolic labeling and SDS-PAGE, and immunoblotting.
authors.affiliation: Lilly Research Laboratories, Indianapolis, Indiana, USA.
authors.forename: M
authors.initials: M
authors.lastname: Bergeron
authors.forename: F R
authors.initials: FR
authors.lastname: Sharp
mesh_terms: D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002477:Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D015262:Xenobiotics / Q000633:toxicity
chemical_list: D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D015262:Xenobiotics