title: Effect of 3α-anderostanediol and indomethacin on acquisition, consolidation and retrieval stage of spatial memory in adult male rats.
abstract: Testosterone and its metabolites have important roles in learning and memory. The current study has conducted to assess the effect of pre-training, post-training and pre-probe trial intrahippocampal CA1 administration of 3α-anderostanediol (one of the metabolites of testosterone) and indomethacin (as 3α-hydroxysteroid dehydrogenase enzyme blocker) on acquisition, consolidation and retrieval in Morris water maze (MWM) task.
  
  
  
  Adult male rats were bilaterally cannulated into CA1 region of hippocampus and then received 3α-diol (0.2, 1, 3 and 6 mug/0.5 mul/side), indomethacin (1.5, 3 and 6 mug/0.5 mul/side), indomethacin (3 mug/0.5 mul/side) + 3α-diol (1 mug/0.5 mul/side), 25-35 min before training, immediately after training and 25-35 min before probe trial in MWM task.
  
  
  
  Our results showed that injection of 3α-diol and indomethacin significantly increased the escape latency and traveled distance to find hidden platform in acquisition and consolidation stage, but did not have any effect on retrieval of spatial learning as compared with the control group.
  
  
  
  It is concluded that intra-CA1 administration of 3α-diol and indomethacin could impair spatial learning and memory in acquisition and consolidation stage. Also, intrahippocampal injection of indomethacin + 3α-diol could not change spatial learning and memory impairment effect of indomethacin or 3α-diol in MWM task.
authors.affiliation: Dept. of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran. naghdi@pasteur.ac.ir
authors.forename: Somayeh
authors.initials: S
authors.lastname: Assadian Narenji
authors.affiliation: Dept. of Physiology 
  and Pharmacology, Pasteur Institute of Iran, Tehran 13164, Iran.
authors.forename: Nasser
authors.initials: N
authors.lastname: Naghdi
authors.affiliation: Dept. of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran. naghdi@pasteur.ac.ir
authors.forename: Shahrbano
authors.initials: S
authors.lastname: Oryan
authors.affiliation: Dept. of Virology, 
  Pasteur Institute of Iran, Tehran 13164, Iran.
authors.forename: Kayhan
authors.initials: K
authors.lastname: Azadmanesh
mesh_terms: D000375:Aging / Q000502:physiology*; D000731:Androstanes / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000502:physiology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D007213:Indomethacin / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000731:Androstanes; D007213:Indomethacin; D004121:Dimethyl Sulfoxide