title: Environmental control of the calicheamicin polyketide synthase leads to detection of a programmed octaketide and a proposal for enediyne biosynthesis.
abstract: A light in the dark: the fermentation products of the polyketide synthase CalE8 (without its cognate thioesterase) were identified and gave some insight into the elusive early steps of calicheamicin biosynthesis. Fermentation in either the light or dark resulted in different proportions of a new octaketide and led to an updated mechanistic proposal.
authors.affiliation: Department of Chemistry, The Johns Hopkins University, Baltimore, MD 21218, USA.
authors.forename: Katherine
authors.initials: K
authors.lastname: Belecki
authors.forename: Craig A
authors.initials: CA
authors.lastname: Townsend
mesh_terms: D053281:Enediynes / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D048630:Polyketide Synthases / Q000096:biosynthesis / Q000378:metabolism*; D061065:Polyketides / Q000032:analysis / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: D053281:Enediynes; D061065:Polyketides; D048630:Polyketide Synthases