title: Comparison of commercial enzyme-linked immunosorbent assay kits with agar gel precipitation and hemagglutination-inhibition tests for detecting antibodies to avian influenza viruses.
abstract: We evaluated the utility of 5 commercial enzyme-linked immunosorbent assay (ELISA) kits for detecting antibodies to avian influenza viruses. The sensitivities and specificities of the ELISA kits were compared with those of the agar gel precipitation (AGP) and hemagglutination-inhibition (HI) tests. The results suggest that some ELISA kits might not be suitable for monitoring during the early stages of avian influenza virus infections. Therefore, ELISA kits should only be used in conjunction with a profound knowledge about monitoring of avian influenza.
authors.affiliation: Research Institute for Animal Science in Biochemistry & Toxicology, 3-7-11 Hashimotodai, Midori-ku, Sagamihara, Kanagawa 252-0132, Japan. r_shiraishi@riasbt.or.jp
authors.forename: Rikiya
authors.initials: R
authors.lastname: Shiraishi
authors.forename: Akiko
authors.initials: A
authors.lastname: Nishiguchi
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsukamoto
authors.forename: Masatake
authors.initials: M
authors.lastname: Muramatsu
mesh_terms: D000362:Agar; D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D001717:Birds; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D005782:Gels; D006385:Hemagglutination Inhibition Tests / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D011933:Reagent Kits, Diagnostic / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D005782:Gels; D011933:Reagent Kits, Diagnostic; D000362:Agar