title: The usefulness of the buffy coat smear and panbacterial polymerase chain reaction in early diagnosis of neonatal sepsis.
abstract: OBJECTIVE
  In this study are evaluated the usefulness of the buffy coat smear and panbacterial polymerase chain reaction (PCR) as diagnostic tests in the early detection of neonatal sepsis.
  
  
  METHODS
  It was studied 49 patients aged up to 28 days who were hospitalized in the Intensive Care Unit (ICUs) of the Neonatology, with a clinical diagnosis of neonatal sepsis and 49 umbilical cord samples of healthy newborns. Blood cultures and 50 microL of plasma were taken for the DNA and performance of the broad-range PCR primer system (panbacterial PCR). Simultaneously, were taken three capillaries with blood for the leukocyte layer (buffy coat) smear, we performed three stains: Gram; Löeffler blue methylene (LBM), and acridine orange (AO). Statistical analysis included sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) against the clinical diagnosis.
  
  
  RESULTS
  With respect to stains of buffy coat smear, they resulted very specific, from 90-97%, with 64-75% sensitivity, 87-94% PPV, and 77-82% NPV. In inverse fashion, PCR resulted very sensitive at 96%, with 91% specificity, 92% PPV, and 96% NPV.
  
  
  CONCLUSIONS
  Buffy coat smear stains are easy, fast, and specific, while that of PCR was highly sensitive. Thus, both can be utilized as diagnostic tests.
authors.affiliation: Unidad de Investigaci6n en Inmunología e Infectología, Centro Medico Nacional La Raza, Instituto Mexicano del Seguro Social, México, D.F. gelorriaga@webtelmex.net.mx
authors.forename: Guadalupe
authors.initials: G
authors.lastname: García-Elorriaga
authors.forename: Nancy
authors.initials: N
authors.lastname: Cortés-Torres
authors.forename: Julio César
authors.initials: JC
authors.lastname: Ballesteros-Del-Olmo
authors.forename: Guillermo
authors.initials: G
authors.lastname: Del Rey-Pineda
authors.forename: César
authors.initials: C
authors.lastname: Gonzáez-Bonilla
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D018805:Sepsis / Q000097:blood* / Q000175:diagnosis*; D013194:Staining and Labeling