title: Detection of nitrosated proteins.
abstract: Three methods are provided in this unit to detect nitrosated proteins. The Saville assay is the most commonly used and has the advantages that it is less sensitive to interference than the quinine sulfate-based fluorescent assay, uses simple chemical reagents, and requires a spectrophotometer. The diaminonaphthalene assay (a fluorescence assay) and chemiluminescence assay are used when the experiment requires high sensitivity. Mass spectrometry is employed for precise structural determinations.
authors.affiliation: Boston University School of Medicine, Boston, Massachusetts, USA.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Loscalzo
mesh_terms: D003545:Cysteine / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D057230:Limit of Detection; D015538:Nitrosation; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D026403:S-Nitrosothiols / Q000032:analysis / Q000737:chemistry; D014364:Tryptophan / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry
chemical_list: C500105:4-nitro-tryptophan; D011506:Proteins; D026403:S-Nitrosothiols; D014364:Tryptophan; C021315:S-nitrosocysteine; D003545:Cysteine