title: Differential expression of anthocyanin biosynthetic genes and transcription factor PcMYB10 in pears (Pyrus communis L.).
abstract: Anthocyanin biosynthesis in various plants is affected by environmental conditions and controlled by the transcription level of the corresponding genes. In pears (Pyrus communis cv. 'Wujiuxiang'), anthocyanin biosynthesis is significantly induced during low temperature storage compared with that at room temperature. We further examined the transcriptional levels of anthocyanin biosynthetic genes in 'Wujiuxiang' pears during developmental ripening and temperature-induced storage. The expression of genes that encode flavanone 3-hydroxylase, dihydroflavonol 4-reductase, anthocyanidin synthase, UDP-glucose: flavonoid 3-O-glucosyltransferase, and R2R3 MYB transcription factor (PcMYB10) was strongly positively correlated with anthocyanin accumulation in 'Wujiuxiang' pears in response to both developmental and cold-temperature induction. Hierarchical clustering analysis revealed the expression patterns of the set of target genes, of which PcMYB10 and most anthocyanin biosynthetic genes were related to the same cluster. The present work may help explore the molecular mechanism that regulates anthocyanin biosynthesis and its response to abiotic stress at the transcriptional level in plants.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Ban
authors.forename: Xi-Hong
authors.initials: XH
authors.lastname: Li
authors.forename: Mao-Yu
authors.initials: MY
authors.lastname: Wu
authors.forename: Ai-Li
authors.initials: AL
authors.lastname: Wang
authors.forename: Yu-Qian
authors.initials: YQ
authors.lastname: Jiang
authors.forename: Yun-Hong
authors.initials: YH
authors.lastname: Jiang
mesh_terms: D000872:Anthocyanins / Q000235:genetics*; D016000:Cluster Analysis; D003080:Cold Temperature; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D010940:Plant Proteins / Q000235:genetics*; D031989:Pyrus / Q000235:genetics* / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins; D014157:Transcription Factors