title: Effects of umbilical cord serum eye drops in a mouse model of ocular chemical burn.
abstract: OBJECTIVE
  To evaluate the effect of umbilical cord serum (UCS) eye drops on corneal wound healing and haze in a mouse model of ocular chemical burn and compare with that of peripheral blood serum (PBS) eye drops or artificial tears (AT).
  
  
  METHODS
  Chemical burn of the ocular surface was induced by 1N NaOH in C57BL/6 mice. Injured mice were topically treated with 20% UCS, 20% PBS, or AT four times daily. The changes of corneal epithelial defects and degree of haze were analyzed at 6 h, 1, 2, 3, 5, and 7 days, and histological examination was performed at 3 and 7 days. The concentration of IL-1β in the cornea was measured by enzyme-linked immunosorbent assay at 7 days after treatment.
  
  
  RESULTS
  The UCS group showed lower epithelial defect parameters compared with the PBS group at 1 and 2 days (p < 0.05), and with the AT group from 1-5 days (p < 0.05). The haze scores were significantly lower in the UCS group than in the PBS group at 2 and 3 days (p < 0.05), and in the AT group from 2-7 days (p < 0.05). Histological examination showed better epithelial integrity and lower stromal inflammation and edema in the UCS group than the other groups. IL-1β levels were 99.71 ± 85.22 and 230.76 ± 102.67 pg/ml in the UCS and PBS groups, respectively (p = 0.03).
  
  
  CONCLUSIONS
  UCS eye drops are more effective in improving corneal wound healing and reducing corneal haze compared with PBS eye drops and AT in experimental chemical burns.
authors.affiliation: Department of Ophthalmology, Research Institute of Medical Sciences, Chonnam National University Medical School and Hospital, Gwangju, Korea.
authors.forename: Han-Jin
authors.initials: HJ
authors.lastname: Oh
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Jang
authors.forename: Zhengri
authors.initials: Z
authors.lastname: Li
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Yoon
mesh_terms: D000818:Animals; D002057:Burns, Chemical / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D003316:Corneal Diseases / Q000139:chemically induced / Q000378:metabolism / Q000628:therapy*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D019573:Epithelium, Corneal / Q000378:metabolism; D005126:Eye Burns / Q000139:chemically induced*; D005312:Fetal Blood / Q000502:physiology*; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009883:Ophthalmic Solutions; D012972:Sodium Hydroxide / Q000633:toxicity; D014945:Wound Healing / Q000502:physiology*
chemical_list: D053583:Interleukin-1beta; D009883:Ophthalmic Solutions; D012972:Sodium Hydroxide