title: [Chromosomal polymorphism of Acremonium chrysogenum strains producing cephalosporin C].
abstract: Using pulse electrophoresis in controlled homogenous electric field we conducted molecular karyotyping of highly-productive and laboratory strains of Acremonium chrysogenum generating antibiotic cephalosporin C (cefC). Differences in size of several chromosomes of highly active strain CB26/8 compared to the wild-type strain ATCC 11550 were revealed. It was shown that chromosomal polymorphism in the highly active strain was not associated with alteration of localization and copy number ofcephalosporin C biosynthesis and transport genes. A cluster of "early" cefC biosynthesis genes is located on chromosome VI (4.4 Mb); a cluster of the "late genes", on chromosome II (2.3 Mb). Both clusters are presented as a single copy perA. chrysogenum genome in the wild-type and in CB26/8 producer strains. Based on comparative analysis of laboratory and industrial cefC producers, a karyotype scheme for A. chrysogenum strains of various origins was designed.
authors.forename: M V
authors.initials: MV
authors.lastname: Dumina
authors.forename: A A
authors.initials: AA
authors.lastname: Zhgun
authors.forename: A G
authors.initials: AG
authors.lastname: Domracheva
authors.forename: M I
authors.initials: MI
authors.lastname: Novak
authors.forename: M A
authors.initials: MA
authors.lastname: El'darov
mesh_terms: D000164:Acremonium / Q000166:cytology / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D002511:Cephalosporins / Q000096:biosynthesis*; D015825:Chromosomes, Fungal / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D059785:Karyotype; D011110:Polymorphism, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; C025163:cephalosporin C