title: The effects of α-tocopherol on oxidative damage and serum levels of Clara cell protein 16 in aspiration pneumonitis induced by bile acids.
abstract: Our aim in this study is to examine the effects of α-tocopherol (AT) on rats with aspiration pneumonitis induced with bile acids (BAs). The animals were divided in to four groups, namely saline group (n = 7), saline + AT group (n = 7), BA group (n = 7), and BA + AT group (n = 7). Saline and BA groups aspirated intratracheally with 1 ml/kg saline and 1 ml/kg bile acids, respectively. AT was given at 20 mg/kg/day dosage for 7 days to the groups. AT group was given 20 mg/kg/day AT for 7 days. Malondialdehyde (MDA), Clara cell protein 16 (CC-16), catalase (CAT), superoxide dismutase (SOD), as well as peribronchial inflammatory cell infiltration, alveolar septal infiltration, alveolar edema, alveolar exudate, alveolar histiocytes, and necrosis were evaluated. The CAT activity of the BA group was significantly lower than the saline group. In the BA + AT group, there was a significant increase in SOD and CAT activities when compared with that of the BA group. The CC-16 and MDA contents in the BA group were significantly higher than in the saline group. The CC-16 and MDA levels of the BA + AT group were significantly lower than BA group. Histopathologic changes were seen in BA group, and there was a significant decrease in the BA + AT group. In conclusion, AT might be beneficial in the treatment of aspiration pneumonitis induced by BAs because AT decreased oxidative damage and resulted in a decrease in CC-16 levels.
authors.affiliation: Department of Medical Biochemistry, Ondokuz Mayis University, Samsun, Turkey. hasanalacam@hotmail.com
authors.forename: H
authors.initials: H
authors.lastname: Alaçam
authors.forename: R
authors.initials: R
authors.lastname: Karli
authors.forename: O
authors.initials: O
authors.lastname: Alici
authors.forename: B
authors.initials: B
authors.lastname: Avci
authors.forename: A
authors.initials: A
authors.lastname: Güzel
authors.forename: A
authors.initials: A
authors.lastname: Kozan
authors.forename: C
authors.initials: C
authors.lastname: Mertoglu
authors.forename: N
authors.initials: N
authors.lastname: Murat
authors.forename: O
authors.initials: O
authors.lastname: Salis
authors.forename: A
authors.initials: A
authors.lastname: Güzel
authors.forename: M
authors.initials: M
authors.lastname: Sahin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001647:Bile Acids and Salts; D005260:Female; D008315:Malondialdehyde / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D011015:Pneumonia, Aspiration / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood; D014598:Uteroglobin / Q000097:blood; D024502:alpha-Tocopherol / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D001647:Bile Acids and Salts; C556391:Scgb1a1 protein, rat; D008315:Malondialdehyde; D014598:Uteroglobin; D013482:Superoxide Dismutase; D024502:alpha-Tocopherol