title: Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran.
abstract: Cystic echinococcosis is endemic in Iran, particularly in Ardabil Province, where it causes health and economic problems. The genetic pattern of Echinococcus granulosus has been determined in most parts of Iran, except in this area. In the present investigation, 55 larval isolates were collected from humans (11), sheep (19), goats (4) and cattle (21). For analysis of the genetic characteristics of E. granulosus isolates, DNA sequencing of mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes was applied. Fifty isolates were successfully analysed, with 92% (46) and 8% (4) identified as G1 and G3 genotypes, respectively. The sequence analyses of the isolates displayed nine characteristic profiles in cox1 sequences and eight characteristic profiles in nad1 sequences. Based on these results, the sheep strain (G1 genotype) was the most prevalent in humans, sheep, goats and cattle. The buffalo strain (G3 genotype) was not only demonstrated in sheep (1 isolate) and cattle (1 isolate), but also for the first time in two human isolates. These findings will provide information for local control of echinococcosis.
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Pezeshki
authors.forename: L
authors.initials: L
authors.lastname: Akhlaghi
authors.forename: M
authors.initials: M
authors.lastname: Sharbatkhori
authors.forename: E
authors.initials: E
authors.lastname: Razmjou
authors.forename: H
authors.initials: H
authors.lastname: Oormazdi
authors.forename: M
authors.initials: M
authors.lastname: Mohebali
authors.forename: A R
authors.initials: AR
authors.lastname: Meamar
mesh_terms: D000818:Animals; D000829:Animals, Domestic / Q000469:parasitology*; D002417:Cattle; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004443:Echinococcosis / Q000469:parasitology* / Q000662:veterinary*; D048209:Echinococcus granulosus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007492:Iran; D007814:Larva / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D018509:DNA, Helminth; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV