title: Transcriptional organization and regulatory elements of a Pseudomonas sp. strain ADP operon encoding a LysR-type regulator and a putative solute transport system.
abstract: The atzS-atzT-atzU-atzV-atzW gene cluster of the Pseudomonas sp. strain ADP atrazine-degradative plasmid pADP-1, which carries genes for an outer membrane protein and the components of a putative ABC-type solute transporter, is located downstream from atzR, which encodes the LysR-type transcriptional regulator of the cyanuric acid-degradative operon atzDEF. Here we describe the transcriptional organization of these genes. Our results show that all six genes are cotranscribed from the PatzR promoter to form the atzRSTUVW operon. A second, stronger promoter, PatzT, is found within atzS and directs transcription of the four distal genes. PatzT is σ(N) dependent, activated by NtrC in response to nitrogen limitation with the aid of IHF, and repressed by AtzR. A combination of in vivo mutational analysis and primer extension allowed us to locate the PatzT promoter and map the transcriptional start site. Similarly, we used deletion and point mutation analyses, along with in vivo expression studies and in vitro binding assays, to locate the NtrC, IHF, and AtzR binding sites and address their functionality. Our results suggest a regulatory model in which NtrC activates PatzT transcription via DNA looping, while AtzR acts as an antiactivator that diminishes expression by interfering with the activation process.
authors.affiliation: Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, Consejo Superior de Investigaciones Científicas, Junta de Andalucía, Seville, Spain.
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Platero
authors.forename: Manuel
authors.initials: M
authors.lastname: García-Jaramillo
authors.forename: Eduardo
authors.initials: E
authors.lastname: Santero
authors.forename: Fernando
authors.initials: F
authors.lastname: Govantes
mesh_terms: D004252:DNA Mutational Analysis; D015964:Gene Expression Regulation, Bacterial; D026901:Membrane Transport Proteins / Q000235:genetics*; D005810:Multigene Family; D009876:Operon; D010957:Plasmids; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D011549:Pseudomonas / Q000235:genetics*; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D026901:Membrane Transport Proteins; D014157:Transcription Factors