title: Transcriptional differences between rhesus embryonic stem cells generated from in vitro and in vivo derived embryos.
abstract: Numerous studies have focused on the transcriptional signatures that underlie the maintenance of embryonic stem cell (ESC) pluripotency. However, it remains unclear whether ESC retain transcriptional aberrations seen in in vitro cultured embryos. Here we report the first global transcriptional profile comparison between ESC generated from either in vitro cultured or in vivo derived primate embryos by microarray analysis. Genes involved in pluripotency, oxygen regulation and the cell cycle were downregulated in rhesus ESC generated from in vitro cultured embryos (in vitro ESC). Significantly, several gene differences are similarly downregulated in preimplantation embryos cultured in vitro, which have been associated with long term developmental consequences and disease predisposition. This data indicates that prior to derivation, embryo quality may influence the molecular signature of ESC lines, and may differentially impact the physiology of cells prior to or following differentiation.
authors.affiliation: Department of Physiology, Wayne State University, Detroit, Michigan, United States of America. ajharvey@unimelb.edu.au
authors.forename: Alexandra J
authors.initials: AJ
authors.lastname: Harvey
authors.forename: Shihong
authors.initials: S
authors.lastname: Mao
authors.forename: Claudia
authors.initials: C
authors.lastname: Lalancette
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Krawetz
authors.forename: Carol A
authors.initials: CA
authors.lastname: Brenner
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D016000:Cluster Analysis; D004622:Embryo, Mammalian / Q000196:embryology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D008253:Macaca mulatta; D010101:Oxygen Consumption / Q000235:genetics; D015203:Reproducibility of Results; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D014157:Transcription Factors