title: Enzyme-regulated unmodified gold nanoparticle aggregation: a label free colorimetric assay for rapid and sensitive detection of adenosine deaminase activity and inhibition.
abstract: Based on enzyme-regulated unmodified gold nanoparticle aggregation, a visual and homogeneous assay of adenosine deaminase (ADA) activity without any other coupling enzymes, aptamers or additional modifications has been developed. The present strategy is simple, cost-effective, high throughput, selective and sensitive for ADA with a detection limit of 0.8227 U L(-1).
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Zhang
authors.forename: Jingjin
authors.initials: J
authors.lastname: Zhao
authors.forename: Jianhui
authors.initials: J
authors.lastname: Jiang
authors.forename: Ruqin
authors.initials: R
authors.lastname: Yu
mesh_terms: D000243:Adenosine Deaminase / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry; D057075:Enzyme Assays / Q000191:economics; D004791:Enzyme Inhibitors / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D006046:Gold; D000243:Adenosine Deaminase