title: Iterative plug-and-play methodology for constructing and modifying synthetic gene networks.
abstract: We present a methodology for the design, construction and modification of synthetic gene networks. This method emphasizes post-assembly modification of constructs based on network behavior, thus facilitating iterative design strategies and rapid tuning and repurposing of gene networks. The ease of post-construction modification afforded by this approach and the ever-increasing repository of components within the framework will help accelerate the development of functional genetic circuits for synthetic biology.
authors.affiliation: Howard Hughes Medical Institute, Boston University, Boston, Massachusetts, USA.
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Litcofsky
authors.forename: Raffi B
authors.initials: RB
authors.lastname: Afeyan
authors.forename: Russell J
authors.initials: RJ
authors.lastname: Krom
authors.forename: Ahmad S
authors.initials: AS
authors.lastname: Khalil
authors.forename: James J
authors.initials: JJ
authors.lastname: Collins
mesh_terms: D019295:Computational Biology / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D053263:Gene Regulatory Networks; D005813:Genes, Synthetic; D058615:Synthetic Biology