title: Protective vaccination against infectious bursal disease virus with whole recombinant Kluyveromyces lactis yeast expressing the viral VP2 subunit.
abstract: Here we report on vaccination approaches against infectious bursal disease (IBD) of poultry that were performed with complete yeast of the species Kluyveromyces lactis (K. lactis). Employing a genetic system that enables the rapid production of stably transfected recombinant K. lactis, we generated yeast strains that expressed defined quantities of the virus capsid forming protein VP2 of infectious bursal disease virus (IBDV). Both, subcutaneous as well as oral vaccination regiments with the heat-inactivated but otherwise untreated yeast induced IBDV-neutralizing antibodies in mice and chickens. A full protection against a subsequent IBDV infection was achieved by subcutaneous inoculation of only milligram amounts of yeast per chicken. Oral vaccination also generated protection: while mortality was observed in control animals after virus challenge, none of the vaccinees died and ca. one-tenth were protected as indicated by the absence of lesions in the bursa of Fabricius. Recombinant K. lactis was thus indicated as a potent tool for the induction of a protective immune response by different applications. Subcutaneously applied K. lactis that expresses the IBDV VP2 was shown to function as an efficacious anti-IBD subunit vaccine.
authors.affiliation: Institute of Biochemistry and Biotechnology, Faculty of Life Sciences (NFI), Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Marina
authors.initials: M
authors.lastname: Arnold
authors.forename: Vijay
authors.initials: V
authors.lastname: Durairaj
authors.forename: Egbert
authors.initials: E
authors.lastname: Mundt
authors.forename: Katja
authors.initials: K
authors.lastname: Schulze
authors.forename: Karin D
authors.initials: KD
authors.lastname: Breunig
authors.forename: Sven-Erik
authors.initials: SE
authors.lastname: Behrens
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D002645:Chickens / Q000276:immunology / Q000821:virology*; D005260:Female; D015870:Gene Expression; D023061:Gene Order; D018390:Gene Targeting; D007243:Infectious bursal disease virus / Q000235:genetics / Q000276:immunology*; D007279:Injections, Subcutaneous; D007716:Kluyveromyces / Q000235:genetics*; D051379:Mice; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: C082513:VP2 protein, infectious bursal disease virus; D015164:Vaccines, Inactivated; D015678:Viral Structural Proteins; D014765:Viral Vaccines