title: Primer design for identifying economically important Liriomyza species (Diptera: Agromyzidae) by multiplex PCR.
abstract: Leafminer flies, especially, Liriomyza huidobrensis, Liriomyza sativae and Liriomyza trifolii, are quarantine species in many countries. Their morphological similarity makes identification difficult. To develop a rapid, reliable, sensitive and simple molecular identification method using multiplex PCR, we newly sequenced the mitochondrial cytochrome oxidase I (COI) genes of Liriomyza bryoniae, Liriomyza chinensis, L. huidobrensis, L. sativae, L. trifolii, Chromatomyia horticola and four parasitoid species. We aligned them with all the COI sequences of the leafminer flies found in the international DNA nucleotide sequence databases (DDBJ/EMBL/GenBank). We then designed species-specific primers to allow us to differentiate between L. bryoniae, L. chinensis, L. huidobrensis, L. sativae, and L. trifolii.
authors.affiliation: Miyagi Prefectural Institute of Agriculture and Horticulture, Takadate-kawakami, Natori, Miyagi 981-1243, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Toshio
authors.initials: T
authors.lastname: Masuda
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mochizuki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Konishi
authors.forename: Susumu
authors.initials: S
authors.lastname: Tokumaru
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Ueno
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics*; D004175:Diptera / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D003576:Electron Transport Complex IV