title: Second harmonic generation microscopy as a powerful diagnostic imaging modality for human ovarian cancer.
abstract: In this study we showed that second-harmonic generation (SHG) microscopy combined with precise methods for images evaluation can be used to detect structural changes in the human ovarian stroma. Using a set of scoring methods (alignment of collagen fibers, anisotropy, and correlation), we found significant differences in the distribution and organization of collagen fibers in the stroma component of serous, mucinous, endometrioid and mixed ovarian tumors as compared with normal ovary tissue. This methodology was capable to differentiate between cancerous and healthy tissue, with clear cut distinction between normal, benign, borderline, and malignant tumors of serous type. Our results indicated that the combination of different image-analysis approaches presented here represent a powerful tool to investigate collagen organization and extracellular matrix remodeling in ovarian tumors.
authors.affiliation: Biomedical Lasers Application Laboratory, Optics and Photonics Research Center, "Gleb Wataghin" Institute of Physics, State University of Campinas UNICAMP, Brazil; Microscopy Laboratory Applied to Molecular and Cellular Studies, School of Bioengineering, National University of Entre Ríos UNER, Ruta 11 Km10, Oro Verde 3101, Entre Ríos, Argentina. jadur@bioingenieria.edu.ar.
authors.forename: Javier
authors.initials: J
authors.lastname: Adur
authors.forename: Vitor B
authors.initials: VB
authors.lastname: Pelegati
authors.forename: Andre A
authors.initials: AA
authors.lastname: de Thomaz
authors.forename: Mariana O
authors.initials: MO
authors.lastname: Baratti
authors.forename: Liliana A L A
authors.initials: LA
authors.lastname: Andrade
authors.forename: Hernandes F
authors.initials: HF
authors.lastname: Carvalho
authors.forename: Fátima
authors.initials: F
authors.lastname: Bottcher-Luiz
authors.forename: Carlos Lenz
authors.initials: CL
authors.lastname: Cesar
mesh_terms: D003094:Collagen / Q000378:metabolism; D003952:Diagnostic Imaging / Q000379:methods*; D005260:Female; D006801:Humans; D008853:Microscopy / Q000379:methods*; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology
chemical_list: D003094:Collagen