title: Antibacterial, Antifungal, Cytotoxic, Phytotoxic, Insecticidal, and Enzyme Inhibitory Activities of Geranium wallichianum.
abstract: The present study describes the phytochemical investigations of the crude extracts of rhizomes and leaves of Geranium wallichianum. The crude extracts were fractionated to obtain n-hexane, ethyl acetate, and n-butanol fractions, which were subjected to different biological activities and enzyme inhibition assays to explore the therapeutic potential of this medicinally important herb. The results indicated that the crude extracts and different fractions of rhizomes and leaves showed varied degree of antimicrobial activities and enzyme inhibitions in different assays. Overall, the rhizome extract and its different fractions showed comparatively better activities in various assays. Furthermore, the purified constituents from the repeated chromatographic separations were also subjected to enzyme inhibition studies against three different enzymes. The results of these studies showed that lipoxygenase enzyme was significantly inhibited as compared to urease. In case of chemical constituents, the sterols (2-4) showed no inhibition, while ursolic acid (1) and benzoic ester (6) showed significant inhibition of urease enzymes.
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar 25000, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ismail
authors.forename: Javid
authors.initials: J
authors.lastname: Hussain
authors.forename: Arif-Ullah
authors.initials: AU
authors.lastname: Khan
authors.forename: Abdul Latif
authors.initials: AL
authors.lastname: Khan
authors.forename: Liaqat
authors.initials: L
authors.lastname: Ali
authors.forename: Farman-Ullah
authors.initials: FU
authors.lastname: Khan
authors.forename: Amir Zada
authors.initials: AZ
authors.lastname: Khan
authors.forename: Uzma
authors.initials: U
authors.lastname: Niaz
authors.forename: In-Jung
authors.initials: IJ
authors.lastname: Lee