title: Significant bacterial contamination risk reduction with the use of diversion pouch.
abstract: BACKGROUND
  Significant efforts have been made towards bacterial risk minimization by limiting the chance of bacteria entering into collected blood, detecting its presence and eliminating them by pathogen reduction technology. Improved skin disinfection and the use of diversion pouch are effective upstream measures to reduce the risk of bacterial sepsis. Here we report on our experience with the use of blood bags with diversion pouch.
  
  
  METHODS
  An observational study was performed to compare the bacterial contamination rate for two periods of time, i.e. before and after introduction of diversion pouch into blood bags. The incidence of bacterial contamination was monitored by the existing bacterial surveillance programme using pooled samples from 5 units of random donor platelets underwent aerobic culture in the BacT/ALERT 3D system.
  
  
  RESULTS
  Between 1 June 2004 and 31 May 2006 (blood bag without diversion pouch), 50 (0·0213%) out of 234,252 units of random donor platelets were found to have bacteria on culture whereas 15 (0·0057%) isolates out of 262,156 units were found from 1 June 2007 to 31 May 2009 (after diversion pouch was introduced). Overall, there was an 85% reduction in bacterial contaminated risk due to skin flora (P < 0·0001) but an increasing trend of detection of non skin flora such as Streptococcus bovis was noted.
  
  
  CONCLUSIONS
  Integration of diversion pouch into blood bags to divert the first 30 mL blood during blood collection on top of the current skin disinfection protocol can significantly reduce the risk of bacterial contamination.
authors.affiliation: Hong Kong Red Cross Blood Transfusion Service, Hong Kong SAR, China. ckleea@ha.org.hk
authors.forename: C K
authors.initials: CK
authors.lastname: Lee
authors.forename: H K
authors.initials: HK
authors.lastname: Wong
authors.forename: P L
authors.initials: PL
authors.lastname: Ho
authors.forename: W C
authors.initials: WC
authors.lastname: Tsoi
authors.forename: K Y
authors.initials: KY
authors.lastname: Lee
authors.forename: G T F
authors.initials: GT
authors.lastname: Tsui
authors.forename: E
authors.initials: E
authors.lastname: Chua
authors.forename: J N S
authors.initials: JN
authors.lastname: Leung
authors.forename: C K
authors.initials: CK
authors.lastname: Lin
mesh_terms: D001782:Blood Donors; D058731:Blood Safety; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination; D005260:Female; D006801:Humans; D008297:Male; D017410:Practice Guidelines as Topic; D012867:Skin / Q000382:microbiology*; D016985:Streptococcus bovis