title: Chromosomal aberrations in peripheral blood lymphocytes in patients with newly diagnosed celiac and Crohn's disease.
abstract: OBJECTIVE
  The aims of this research were to determine the number of chromosomal aberrations in peripheral blood lymphocytes and to evaluate the number of circulating lymphocytes with CD103, integrin expressed on intraepithelial lymphocytes and preserved in enteropathy-associated T-cell lymphoma, in patients with newly diagnosed Crohn's disease, celiac disease, and healthy controls.
  
  
  METHODS
  During the period of 30 months, we included 44 patients. Chromosome aberrations were analyzed in peripheral blood lymphocytes by a single cytogeneticist. Multicolor flow cytometric was used for immunophenotyping of peripheral blood lymphocytes.
  
  
  RESULTS
  We found a significantly higher number of chromosomal aberrations/100 metaphases in the celiac and Crohn's disease group compared with the controls (P=0.01) and they also had a significantly higher number of aberrant cells compared with the controls (P<0.001). There was no statistically significant difference between the groups with respect to the percentage of CD103+ and CD8+CD103+ cells between groups (P=0.16 and 0.41, respectively) and no correlation between the total number of chromosomal aberrations and the percentage of CD103+ and CD8+CD103+ cells (P=0.06 and 0.06, respectively).
  
  
  CONCLUSIONS
  Patients with active celiac and newly diagnosed Crohn's disease, before treatment initiation, have a significantly increased number of chromosomal aberrations in peripheral blood lymphocytes. No dissemination of intraepithelial cells in the blood and correlation to the chromosomal aberration was found.
authors.affiliation: Referral Center for Pediatric Gastroenterology and Nutrition, Children's Hospital Zagreb, School of Medicine, University of Zagreb, Zagreb, Croatia. ivahojsak@gmail.com
authors.forename: Iva
authors.initials: I
authors.lastname: Hojsak
authors.forename: Alenka
authors.initials: A
authors.lastname: Gagro
authors.forename: Iskra
authors.initials: I
authors.lastname: Petković
authors.forename: Zrinjka
authors.initials: Z
authors.lastname: Mišak
authors.forename: Sanja
authors.initials: S
authors.lastname: Kolaček
mesh_terms: D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology*; D016022:Case-Control Studies; D002446:Celiac Disease / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D002648:Child; D002869:Chromosome Aberrations; D003424:Crohn Disease / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D020732:Cytogenetic Analysis; D058527:Enteropathy-Associated T-Cell Lymphoma / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D039001:Integrin alpha Chains / Q000378:metabolism; D008297:Male; D011446:Prospective Studies
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D017252:CD3 Complex; D039001:Integrin alpha Chains; C091367:alpha E integrins