title: Amniotic fluid myeloperoxidase in pregnancies complicated by preterm prelabor rupture of membranes.
abstract: OBJECTIVE
  To determine amniotic fluid myeloperoxidase concentration in women with preterm prelabor rupture of the membranes with microbial invasion of the amniotic cavity and histological chorioamnionitis.
  
  
  METHODS
  One hundred eighty-one women with singleton pregnancies with a gestational age between 24+0 and 36+6 weeks were included in this study. Amniocenteses were performed, and myeloperoxidase concentration in the amniotic fluid was determined using ELISA.
  
  
  RESULTS
  Women with microbial invasion of the amniotic cavity had higher median myeloperoxidase concentration than women without this condition (149.2 ng/mL vs. 54.6 ng/mL; p = 0.0006). Women with the presence of histological chorioamnionitis had higher median myeloperoxidase concentration than women without histological chorioamnionitis (103.7 ng/mL vs. 50.0 ng/mL; p = 0.0001). The presence of both microbial invasion of the amniotic cavity and histological chorioamnionitis was associated with higher median myeloperoxidase concentration (456.0 ng/mL vs. 52.9 ng/mL; p < 0.0001). The results remained significant after adjusting for gestational age.
  
  
  CONCLUSIONS
  Increased amniotic fluid myeloperoxidase in microbial invasion of the amniotic cavity and histological chorioamnionitis confirm a role of myeloperoxidase in preterm prelabor rupture of the membranes pathophysiology.
authors.affiliation: Biomedical Research Center, University Hospital Hradec Kralove, Czech Republic. kacermar@fnhk.cz
authors.forename: Marian
authors.initials: M
authors.lastname: Kacerovsky
authors.forename: Vojtech
authors.initials: V
authors.lastname: Tambor
authors.forename: Marie
authors.initials: M
authors.lastname: Vajrychová
authors.forename: Juraj
authors.initials: J
authors.lastname: Lenco
authors.forename: Helena
authors.initials: H
authors.lastname: Hornychova
authors.forename: Ivana
authors.initials: I
authors.lastname: Musilova
authors.forename: Ramkumar
authors.initials: R
authors.lastname: Menon
mesh_terms: D000328:Adult; D000649:Amniocentesis; D000650:Amnion / Q000382:microbiology; D000653:Amniotic Fluid / Q000201:enzymology*; D002821:Chorioamnionitis / Q000382:microbiology / Q000473:pathology; D015331:Cohort Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005322:Premature Rupture of Fetal Membranes / Q000201:enzymology*; D005865:Gestational Age; D006801:Humans; D007231:Infant, Newborn; D009195:Peroxidase / Q000032:analysis*; D011247:Pregnancy; D011256:Pregnancy Outcome; D047928:Premature Birth / Q000201:enzymology; D012189:Retrospective Studies; D018805:Sepsis / Q000201:enzymology
chemical_list: D009195:Peroxidase