title: Measuring electrophile stress.
abstract: Polyunsaturated fatty acids are primary targets during oxidative stress. Diffusible electrophilic α,β-unsaturated aldehydes, such as 4-hydroxynonenal (HNE), have been shown to modify proteins that mediate cell signaling and modify gene expression pathways. We describe a global strategy for identifying the protein targets of HNE modification. A similar approach can be used for any electrophiles derived from an oxidized lipid.
authors.affiliation: Vanderbilt University Medical Center, Nashville, Tennessee, USA.
authors.forename: Keri A
authors.initials: KA
authors.lastname: Tallman
authors.forename: Andrew
authors.initials: A
authors.lastname: Vila
authors.forename: Ned A
authors.initials: NA
authors.lastname: Porter
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: Marnett
mesh_terms: D000447:Aldehydes / Q000737:chemistry* / Q000633:toxicity; D057930:Click Chemistry / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000633:toxicity; D050356:Lipid Metabolism / Q000187:drug effects / Q000502:physiology; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D011506:Proteins / Q000737:chemistry*
chemical_list: D000447:Aldehydes; D003432:Cross-Linking Reagents; D011506:Proteins; C027576:4-hydroxy-2-nonenal