title: Gene expression analysis using a high-resolution DNA microarray of peripheral whole blood immediately before and after leukocytapheresis for rheumatoid arthritis.
abstract: Leukocytapheresis (LCAP) is a safe, unique therapy pertaining to intractable rheumatoid arthritis (RA) even in cases of drug allergy or infectious states. To investigate how to represent LCAP efficacy, we have conducted gene expression analyses from the peripheral blood of RA patients treated with non-woven polyethylene terephthalate filters. Peripheral blood samples were collected immediately before and after treatment from eight RA patients who received LCAP. Among these patients, all of them achieved 20% improvement in the core set of the American College of Rheumatology (ACR20), and thus, they were confirmed as LCAP responders. Gene expression analysis was done with a high-resolution DNA microarray. The results of each of the two groups' gene expression values (immediately before and after LCAP) were calculated using Welch's t-test. Calculations were performed with a statistical software R.basic package: if the P-value was less than 0.05, this was seen as a significant change. In a comparison of 25,370 gene expressions, the number of genes showing a P-value < 0.05 in the upregulating group was 2110, and in the downregulating group it was 1864. The results of pathway analysis using the MetaCore program indicate that gene groups work for cytoskeletal remodeling are upregulated, and genes related to immune responses, such as antigens presenting via major histocompatibility complex class I and II, are downregulated just after LCAP. These findings may relate to LCAP efficacy for RA patients, but this needs further investigation.
authors.affiliation: Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan. makio.k@juntendo.ac.jp
authors.forename: Makio
authors.initials: M
authors.lastname: Kusaoi
authors.forename: Ken
authors.initials: K
authors.lastname: Yamaji
authors.forename: Go
authors.initials: G
authors.lastname: Murayama
authors.forename: Misa
authors.initials: M
authors.lastname: Yasui
authors.forename: Risa
authors.initials: R
authors.lastname: Yamada
authors.forename: Ruka
authors.initials: R
authors.lastname: Hishinuma
authors.forename: Takuya
authors.initials: T
authors.lastname: Nemoto
authors.forename: Katsura
authors.initials: K
authors.lastname: Hohtatsu
authors.forename: Michiaki
authors.initials: M
authors.lastname: Kageyama
authors.forename: Toshio
authors.initials: T
authors.lastname: Kawamoto
authors.forename: Kaoru
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Fumio
authors.initials: F
authors.lastname: Sekiya
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kon
authors.forename: Michihiro
authors.initials: M
authors.lastname: Ogasawara
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kempe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsuda
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Takasaki
mesh_terms: D001172:Arthritis, Rheumatoid / Q000628:therapy*; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007937:Leukapheresis / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D016896:Treatment Outcome; D015854:Up-Regulation