title: Proteomic identification of plasma protein tyrosine phosphatase alpha and fibronectin associated with liver fluke, Opisthorchis viverrini, infection.
abstract: Opisthorchiasis caused by Opisthorchis viverrini induces periductal fibrosis via host immune/inflammatory responses. Plasma protein alteration during host-parasite interaction-mediated inflammation may provide potential diagnostic and/or prognostic biomarkers. To search for target protein changes in O. viverrini-infected hamsters, a 1-D PAGE gel band was trypsin-digested and analyzed by a LC-MS/MS-based proteomics approach in the plasma profile of infected hamsters, and applied to humans. Sixty seven proteins were selected for further analysis based on at least two unique tryptic peptides with protein ID score >10 and increased expression at least two times across time points. These proteins have not been previously identified in O. viverrini-associated infection. Among those, proteins involved in structural (19%), immune response (13%), cell cycle (10%) and transcription (10%) were highly expressed. Western blots revealed an expression level of protein tyrosine phosphatase alpha (PTPα) which reached a peak at 1 month and subsequently tended to decrease. Fibronectin significantly increased at 1 month and tended to increase with time, supporting proteomic analysis. PTPα was expressed in the cytoplasm of inflammatory cells, while fibronectin was observed mainly in the cytoplasm of fibroblasts and the extracellular matrix at periductal fibrosis areas. In addition, these protein levels significantly increased in the plasma of O. viverrini-infected patients compared to healthy individuals, and significantly decreased at 2-months post-treatment, indicating their potential as disease markers. In conclusion, our results suggest that plasma PTPα and fibronectin may be associated with opisthorchiasis and the hamster model provides the basis for development of novel diagnostic markers in the future.
authors.affiliation: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Jarinya
authors.initials: J
authors.lastname: Khoontawad
authors.forename: Umawadee
authors.initials: U
authors.lastname: Laothong
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.forename: Porntip
authors.initials: P
authors.lastname: Pinlaor
authors.forename: Jason
authors.initials: J
authors.lastname: Mulvenna
authors.forename: Chaisiri
authors.initials: C
authors.lastname: Wongkham
authors.forename: Puangrat
authors.initials: P
authors.lastname: Yongvanit
authors.forename: Chawalit
authors.initials: C
authors.lastname: Pairojkul
authors.forename: Eimorn
authors.initials: E
authors.lastname: Mairiang
authors.forename: Paiboon
authors.initials: P
authors.lastname: Sithithaworn
authors.forename: Somchai
authors.initials: S
authors.lastname: Pinlaor
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins; D016000:Cluster Analysis; D006224:Cricetinae; D004195:Disease Models, Animal; D005260:Female; D005353:Fibronectins / Q000097:blood*; D006801:Humans; D008099:Liver / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D008297:Male; D009889:Opisthorchiasis / Q000097:blood* / Q000276:immunology; D009891:Opisthorchis / Q000276:immunology; D020543:Proteome; D040901:Proteomics; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4 / Q000097:blood*; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D005353:Fibronectins; D020543:Proteome; D054630:Receptor-Like Protein Tyrosine Phosphatases, Class 4