title: DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules.
abstract: The immunogold technique with anti-diacylglycerol acyltransferase 2 (DGAT2) antibody revealed in A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well as in lipid bodies. This being so, lipid synthesis could take place both in ER and in the lipid bodies. The presence of microtubules around the lipid bodies was evidenced under transmission EM. Detection of tubulin around the lipid bodies using the immunogold technique with anti-a-tubulin is in agreement with the above observations. Connection of lipid bodies with microtubules was also detected by us in other plants where they probably participated in lipid synthesis. A similar phenomenon may take place in A. thaliana.
authors.affiliation: Department of Cytophysiology, University of Lodz, Poland. kwiat@biol.uni.lodz.pl
authors.forename: Maria
authors.initials: M
authors.lastname: Kwiatkowska
authors.forename: Dariusz
authors.initials: D
authors.lastname: Stępiński
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Popłońska
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wojtczak
authors.forename: Justyna T
authors.initials: JT
authors.lastname: Polit
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000196:embryology / Q000201:enzymology* / Q000648:ultrastructure*; D029681:Arabidopsis Proteins / Q000378:metabolism* / Q000648:ultrastructure*; D051048:Diacylglycerol O-Acyltransferase / Q000378:metabolism* / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008055:Lipids / Q000737:chemistry*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D017239:Paclitaxel / Q000494:pharmacology; D018517:Plant Roots / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D012639:Seeds / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure
chemical_list: D029681:Arabidopsis Proteins; D008055:Lipids; C578613:DGAT2 protein, Arabidopsis; D051048:Diacylglycerol O-Acyltransferase; D017239:Paclitaxel