title: Synthesis of 6-amino-2-vinylpurine derivatives for cross-linking and evaluation of the reactivity.
abstract: Oligodeoxynucleotides (ODNs) have been widely used for inhibiting the gene expression in antisense or antigene methods, and the interstrand cross-linking (ICL) forming ODNs have been expected to ensure the inhibition by these methods. Previously, we reported a highly efficient and selective ICL reaction toward cytosine using the 2-amino-6-vinylpurine derivative under acidic conditions. In this Letter, we report the synthesis of ODN containing 6-amino-2-vinylpurine derivatives and evaluation of the cross-linking reactivity.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-shi, Miyagi 980-8577, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Kusano
authors.forename: Tomoya
authors.initials: T
authors.lastname: Sakuraba
authors.forename: Shinya
authors.initials: S
authors.lastname: Hagihara
authors.forename: Fumi
authors.initials: F
authors.lastname: Nagatsugi
mesh_terms: D003432:Cross-Linking Reagents / Q000138:chemical synthesis* / Q000737:chemistry; D003596:Cytosine / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D011687:Purines / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D009838:Oligodeoxyribonucleotides; D011687:Purines; D012313:RNA; D003596:Cytosine; D004247:DNA; C030985:purine