title: The catabolite repression control protein Crc plays a role in the development of antimicrobial-tolerant subpopulations in Pseudomonas aeruginosa biofilms.
abstract: Bacteria form complex surface-attached biofilm communities in nature. Biofilm cells differentiate into subpopulations which display tolerance towards antimicrobial agents. However, the signal transduction pathways regulating subpopulation differentiation in biofilms are largely unelucidated. In the present study, we show that the catabolite repression control protein Crc regulates the metabolic state of Pseudomonas aeruginosa cells in biofilms, and plays an important role in the development of antimicrobial-tolerant subpopulations in P. aeruginosa biofilms.
authors.affiliation: China-Japan Union Hospital, Jilin University, Changchun 130033, PR China.
authors.forename: Lianbo
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.
authors.forename: Wen-Chi
authors.initials: WC
authors.lastname: Chiang
authors.affiliation: China-Japan Union Hospital, Jilin University, Changchun 130033, PR China.
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 637551, Singapore.|Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.
authors.forename: Michael
authors.initials: M
authors.lastname: Givskov
authors.affiliation: Department of International Health, Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.
authors.forename: Tim
authors.initials: T
authors.lastname: Tolker-Nielsen
authors.affiliation: School of Biological Sciences (SBS), Nanyang Technological University, 637551, Singapore.|Singapore Centre on Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, 637551, Singapore.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: China-Japan Union Hospital, Jilin University, Changchun 130033, PR China.
authors.forename: Guang
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms / Q000187:drug effects*; D057465:Catabolite Repression; D004361:Drug Tolerance; D015964:Gene Expression Regulation, Bacterial; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D012097:Repressor Proteins; C101896:crc protein, Pseudomonas