title: Sungkyunkwania multivorans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from seawater from a seaweed farm.
abstract: A Gram-staining-negative, non-flagellated, non-gliding and rod-shaped bacterial strain, designated PDB-16(T), was isolated from seawater from a seaweed farm on the South Sea in Korea, and its taxonomic position was investigated using a polyphasic approach. Strain PDB-16(T) grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain PDB-16(T) formed an independent lineage within the evolutionary radiation encompassed by the family Flavobacteriaceae. Strain PDB-16(T) contained MK-6 as the predominant menaquinone and iso-C15:0, iso-C15:1 G and iso-C17:0 3-OH as the major fatty acids. The major polar lipids detected in strain PDB-16(T) were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain PDB-16(T) was 42.1 mol%. Strain PDB-16(T) exhibited very low 16S rRNA gene sequence similarities of less than 89.7% to the type strains of any bacterial species with validly published names and less than 90.1% to uncultured bacteria clones. The 16S rRNA gene sequence similarity values and the differences in phenotypic properties between strain PDB-16(T) and some phylogenetically related genera were sufficient to support the proposal that strain PDB-16(T) should be distinguished from previously known genera of the family Flavobacteriaceae. On the basis of the data presented, strain PDB-16(T) is considered to represent a new genus and novel species, for which the name Sungkyunkwania multivorans gen. nov., sp. nov. is proposed. The type strain of Sungkyunkwania multivorans is PDB-16(T) (=KCTC 32138(T)=CCUG 62952(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Hye Ryeon
authors.initials: HR
authors.lastname: Gyeong
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
authors.forename: Soo-In
authors.initials: SI
authors.lastname: Kim
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*; D012624:Seaweed; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C042455:menaquinone 6