title: The expression of leucine-rich repeat gene family members in colorectal cancer.
abstract: This study was conducted to evaluate the association of the leucine-rich repeat (LRR) gene family with colorectal cancer (CRC). The expression of members of the LRR gene family were analyzed in 17 CRC specimens and in 59 healthy colorectal tissues by using Human Exon1.0ST microarray, and in 25 CRC specimens and 32 healthy colorectal tissues by U133Plus2.0 microarray. An association was found for 25 genes belonging to the plant-specific (PS) class of LRR genes (P = 0.05 for Exon1.0 ST and P = 0.04 for U133Plus2.0). In both data-sets, in CRC, we found down-regulation of SHOC2 (P < 0.00003) and LRRC28 (P < 0.01) and up-regulation of LRSAM1 (P < 0.000001), while up-regulation of MFHAS1 (P = 0.0005) and down-regulation of WDFY3 (P = 0.026) were found only in the Exon1.0 ST data-set. The PS LLR gene class encodes proteins that activate immune cells and might play a key role in programmed cell death and autophagy. SHOC2 and LRRC28 genes involved in RAS-mediated signaling, which hinders nutrient deprivation-induced autophagy, might be a possible link between the negative control of autophagy and tumorigenesis.
authors.affiliation: Laboratory and Division of Gastroenterology, IRCCS Casa Sollievo della Sofferenza Hospital, Viale Cappuccini n.1, San Giovanni Rotondo (FG), Italy. a.piepoli@operapadrepio.it
authors.forename: Ada
authors.initials: A
authors.lastname: Piepoli
authors.forename: Orazio
authors.initials: O
authors.lastname: Palmieri
authors.forename: Rosalia
authors.initials: R
authors.lastname: Maglietta
authors.forename: Anna
authors.initials: A
authors.lastname: Panza
authors.forename: Elisa
authors.initials: E
authors.lastname: Cattaneo
authors.forename: Anna
authors.initials: A
authors.lastname: Latiano
authors.forename: Endre
authors.initials: E
authors.lastname: Laczko
authors.forename: Annamaria
authors.initials: A
authors.lastname: Gentile
authors.forename: Massimo
authors.initials: M
authors.lastname: Carella
authors.forename: Gianluigi
authors.initials: G
authors.lastname: Mazzoccoli
authors.forename: Nicola
authors.initials: N
authors.lastname: Ancona
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Marra
authors.forename: Angelo
authors.initials: A
authors.lastname: Andriulli
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D001343:Autophagy; D000071183:Autophagy-Related Proteins; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005091:Exons; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D007930:Leucine / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D040081:Protein Array Analysis; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000071183:Autophagy-Related Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; C117302:MFHAS1 protein, human; D008565:Membrane Proteins; D015513:Oncogene Proteins; C112913:SHOC2 protein, human; D014157:Transcription Factors; C499825:WDFY3 protein, human; C489657:LRSAM1 protein, human; D044767:Ubiquitin-Protein Ligases; D007930:Leucine