title: Sensitive spectrofluorimetric determination of tizanidine in pharmaceutical preparations, human plasma and urine through derivatization with dansyl chloride.
abstract: A sensitive spectrofluorimetric method was developed for the determination of tizanidine in human plasma, urine and pharmaceutical preparations. The method is based on reaction of tizanidine with 1-dimethylaminonaphthalene-5-sulphonyl chloride (dansyl chloride) in an alkaline medium to form a highly fluorescent derivative that was measured at 511 nm after excitation at 383 nm. The different experimental parameters affecting the fluorescence intensity of tizanidine was carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the ranges 50-500 and 20-300 ng/mL for plasma and urine, respectively, detection limits of 1.81 and 0.54 ng/mL and quantification limits of 5.43 and 1.62 ng/mL for plasma and urine, respectively. The method presents good performance in terms of linearity, detection and quantification limits, precision, accuracy and specificity. The proposed method was successfully applied for the determination of tizanidine in pharmaceutical preparations. The results obtained were compared with a reference method, using t- and F-tests.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, Turkey. sevgitatar@yahoo.com
authors.forename: Sevgi Tatar
authors.initials: ST
authors.lastname: Ulu
mesh_terms: D003000:Clonidine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D003619:Dansyl Compounds / Q000737:chemistry; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D003619:Dansyl Compounds; D004364:Pharmaceutical Preparations; C023754:tizanidine; D003000:Clonidine; C029038:dansyl chloride