title: Preparation and characterization of monodisperse core-shell Fe3O4@SiO2 microspheres and its application for magnetic separation of nucleic acids from E. coli BL21.
abstract: In this article, we present an easy route to prepare monodisperse core-shell Fe3O4@SiO2 microspheres with uniform size and shape. Their structures and properties were studied by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FT-IR), and vibrating sample magnetometer (VSM), respectively. The results showed that spherical Fe3O4 microspheres with well dispersion have a rough surface and an average diameter (about 500 nm). After the modification with silica, the particles have a well-defined core-shell structure and a much smoother surface and larger particle diameter (about 600 nm). Furthermore, VSM measurements indicated that the as-prepared Fe3O4 and Fe3O4@SiO2 microspheres were superparamagnetic at room temperature and the saturation magnetization (M(s)) were 58.110 emu/g and 33.479 emu/g, respectively. And then, the prepared monodisperse core-shell Fe3O4@SiO2 microspheres were subsequently applied to separate nucleic acids from the bacteria (E. coli BL21) and verified the great application prospects for bioseparation technology of the biomoleculars.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Nanjing 210096, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Chuanyan
authors.initials: C
authors.lastname: Li
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Ningning
authors.initials: N
authors.lastname: Ma
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhuoxuan
authors.initials: Z
authors.lastname: Lu
authors.forename: Zeeshan
authors.initials: Z
authors.lastname: Ali
authors.forename: Zhijiang
authors.initials: Z
authors.lastname: Xi
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Li
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Liang
authors.forename: Hongna
authors.initials: H
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.forename: Lijian
authors.initials: L
authors.lastname: Xu
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Wang
mesh_terms: D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification*; D010316:Particle Size; D016133:Polymerase Chain Reaction / Q000379:methods; D012822:Silicon Dioxide / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D009696:Nucleic Acids; D012822:Silicon Dioxide; D052203:Ferrosoferric Oxide