title: MIC1-MAG1-SAG1 chimeric protein, a most effective antigen for detection of human toxoplasmosis.
abstract: This study describes a Toxoplasma gondii IgG enzyme-linked immunosorbent assay based on a new chimeric antigen containing three immunodominant regions from the MIC1, MAG1, and SAG1 proteins of the parasite and shows that this test is useful for diagnostic purposes and may replace the lysed and whole-cell antigens.
authors.affiliation: Gdańsk University of Technology, Chemical Faculty, Department of Microbiology, Gdańsk, Poland. holec@pg.gda.pl
authors.forename: Lucyna
authors.initials: L
authors.lastname: Holec-Gasior
authors.forename: Bartlomiej
authors.initials: B
authors.lastname: Ferra
authors.forename: Dorota
authors.initials: D
authors.lastname: Drapala
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D010274:Parasitology / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D015815:Cell Adhesion Molecules; D007074:Immunoglobulin G; C105877:MIC1 protein, Toxoplasma gondii; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; C047168:SAG1 antigen, Toxoplasma