title: Overexpression of soybean GmCBL1 enhances abiotic stress tolerance and promotes hypocotyl elongation in Arabidopsis.
abstract: Although extensive studies and remarkable progress have been made with Arabidopsis calcineurin B-like proteins (CBLs), knowledge of their functions in other plant species is still limited. Here we isolated gene GmCBL1 from soybean, a homolog of AtCBL1 in Arabidopsis. GmCBL1 was differentially induced by multiple abiotic stress and plant hormones, and its transcripts were abundant in seedlings and mature roots. We over-expressed GmCBL1 in Arabidopsis and found that it enhanced tolerances to both high salt and drought stresses in the transgenic plants. Overexpression of GmCBL1 also promoted hypocotyl elongation under light conditions. GmCBL1 may regulate stress tolerance through activation of stress-related genes, and may control hypocotyl development by altering the expression of gibberellin biosynthesis-related genes. This study identifies a putative soybean CBL gene that functions in both stress tolerance and light-dependent hypocotyl development.
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, College of Life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan 430074, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Li
authors.forename: Zhao-Shi
authors.initials: ZS
authors.lastname: Xu
authors.forename: Guang-Yuan
authors.initials: GY
authors.lastname: He
authors.forename: Guang-Xiao
authors.initials: GX
authors.lastname: Yang
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Lian-Cheng
authors.initials: LC
authors.lastname: Li
authors.forename: You-Zhi
authors.initials: YZ
authors.lastname: Ma
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000096:biosynthesis / Q000235:genetics; D018546:Hypocotyl / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D008027:Light; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development*; D055049:Salt Tolerance / Q000235:genetics / Q000502:physiology; D013025:Glycine max / Q000235:genetics / Q000378:metabolism*; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*
chemical_list: D005875:Gibberellins