title: Optimization of solid state fermentation conditions for the production of cellulase by Trichoderma reesei.
abstract: Cellulase production studies have been carried out using the fungal strain Trichoderma reesei NCIM 992 by using three different lignocellulosic materials by solid state fermentation (SSF). The effect of basic fermentation parameters (pH, temperature, moisture content, particle size of substrate and moistening agent) on enzyme production was studied. Maximum cellulase production was 2.63 U ml(-1) using wheat bran as substrate. The optimal conditions forcellulase production forwheat bran were found to be: initial moisture content - 70%, initial medium pH-5.0, temperature-30 degrees C, moistening agents (MSS) and particle size of substrate (500 microm).The optimal incubation time for production was six days. Results indicate the scope for further optimization of the production conditions to obtain higher cellulase titres using the strain under SSF.
authors.affiliation: Department of Biotechnology, Institute of Engineering and Technology, Lucknow - 226 021, India. devendrabiochem@gmail.com
authors.forename: Devendra P
authors.initials: DP
authors.lastname: Maurya
authors.forename: Dhananjay
authors.initials: D
authors.lastname: Singh
authors.forename: Durgesh
authors.initials: D
authors.lastname: Pratap
authors.forename: Jitendra P
authors.initials: JP
authors.lastname: Maurya
mesh_terms: D002480:Cellulase / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D013997:Time Factors; D014242:Trichoderma / Q000201:enzymology* / Q000378:metabolism*
chemical_list: D002480:Cellulase