title: Embryonic-derived olfactory ensheathing cells remyelinate focal areas of spinal cord demyelination more efficiently than neonatal or adult-derived cells.
abstract: Transplanted olfactory ensheathing cells (OECs) contribute to functional recovery in a range of CNS injuries by several mechanisms, one of which is potentially their ability to form myelin sheaths. OECs sourced from donors of different ages have been shown to remyelinate in several in vitro and in vivo models. However, the optimal donor age for OEC associated remyelination is unclear. This project directly compared the remyelinating potential of p75 purified OEC transplants from three donor ages. OECs were sourced from the olfactory bulbs of embryonic, neonatal, and adult rats and purified by immunopanning, and their remyelinating potential was directly compared by transplantation into the same adult rat toxin-induced model of spinal cord demyelination. Remyelination efficiency 3 weeks after transplantation was assessed morphologically and by immunostaining. Our results indicate that all donor ages remyelinate; however, this process is most efficiently achieved by embryonic-derived OECs.
authors.affiliation: Wellcome Trust-MRC Cambridge Stem Cell Institute and Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
authors.forename: David J C
authors.initials: DJ
authors.lastname: Coutts
authors.forename: Charlotte E
authors.initials: CE
authors.lastname: Humphries
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.forename: Giles W
authors.initials: GW
authors.lastname: Plant
authors.forename: Robin J M
authors.initials: RJ
authors.lastname: Franklin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003711:Demyelinating Diseases / Q000378:metabolism / Q000628:therapy*; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D009186:Myelin Sheath / Q000502:physiology; D009416:Nerve Regeneration; D009830:Olfactory Bulb / Q000166:cytology / Q000637:transplantation*; D051381:Rats; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000601:surgery*; D014184:Transplantation, Homologous
chemical_list: D049452:Green Fluorescent Proteins