title: Analysis of biomarkers in a Czech population exposed to heavy air pollution. Part I: bulky DNA adducts.
abstract: The health of human populations living in industrial regions is negatively affected by exposure to environmental air pollutants. In this study, we investigated the impact of air pollution on a cohort of subjects living in Ostrava, a heavily polluted industrial region and compared it with a cohort of individuals from the relatively clean capital city of Prague. This study consisted of three sampling periods differing in the concentrations of major air pollutants (winter 2009, summer 2009 and winter 2010). During all sampling periods, the study subjects from Ostrava region were exposed to significantly higher concentrations of benzo[a]pyrene (B[a]P) and benzene than the subjects in Prague as measured by personal monitors. Pollution by B[a]P, particulate matter of aerodynamic diameter <2.5 µm (PM2.5) and benzene in the Ostrava region measured by stationary monitors was also higher than in Prague, with the exception of PM2.5 in summer 2009 when concentration of the pollutant was significantly elevated in Prague. To evaluate DNA damage in subjects from both locations we determined the levels of bulky DNA adducts in peripheral blood lymphocytes using the (32)P-postlabeling method. Despite higher B[a]P air pollution in the Ostrava region during all sampling periods, the levels of B[a]P-like DNA adducts per 10(8) nucleotides were significantly higher in the Ostrava subjects only in winter 2009 (mean ± SD: 0.21 ± 0.06 versus 0.28 ± 0.08 adducts/10(8) nucleotides, P < 0.001 for Prague and Ostrava subjects, respectively; P < 0.001). During the other two sampling periods, the levels of B[a]P-like DNA adducts were significantly higher in the Prague subjects (P < 0.001). Multivariate analyses conducted among subjects from Ostrava and Prague separately during all sampling periods revealed that exposure to B[a]P and PM2.5 significantly increased levels of B[a]P-like DNA adducts in the Ostrava subjects, but not in subjects from Prague.
authors.affiliation: Institute of Experimental Medicine AS CR, Videnska 1083, 142 20 Prague 4, Czech Republic. prossner@biomed.cas.cz
authors.forename: Pavel
authors.initials: P
authors.lastname: Rossner
authors.forename: Vlasta
authors.initials: V
authors.lastname: Svecova
authors.forename: Jana
authors.initials: J
authors.lastname: Schmuczerova
authors.forename: Alena
authors.initials: A
authors.lastname: Milcova
authors.forename: Nana
authors.initials: N
authors.lastname: Tabashidze
authors.forename: Jan
authors.initials: J
authors.lastname: Topinka
authors.forename: Anna
authors.initials: A
authors.lastname: Pastorkova
authors.forename: Radim J
authors.initials: RJ
authors.lastname: Sram
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000032:analysis* / Q000633:toxicity; D000397:Air Pollution / Q000009:adverse effects*; D001564:Benzo(a)pyrene / Q000633:toxicity; D015415:Biomarkers / Q000032:analysis / Q000097:blood*; D002947:Cities; D018153:Czech Republic; D018736:DNA Adducts / Q000097:blood*; D004784:Environmental Monitoring / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D052638:Particulate Matter / Q000009:adverse effects / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D012621:Seasons; D014815:Vitamins / Q000097:blood
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D018736:DNA Adducts; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; D014815:Vitamins; D001564:Benzo(a)pyrene