title: Evolutionary screening and adsorption behavior of engineered M13 bacteriophage and derived dodecapeptide for selective decoration of gold interfaces.
abstract: There is a growing interest in identifying biomacromolecules such as proteins and peptides to functionalize metallic surfaces through noncovalent binding. One method for functionalizing materials without fundamentally changing their inherent structure is using biorecognition moieties. Here, we proved a general route to select a biomolecule adhesive motif for surface functionalization by comprehensively screening phage displayed peptides. In particular, we selected a genetically engineered M13 bacteriophage and a linear dodecapeptide derived from its pIII domain for recognizing gold surfaces in a specific and selective manner. In the phage context, we demonstrated the adhesive motif was capable to adsorb on gold in a preferential way with a morphological and viscoelastic signature of the adsorbed layer as evidenced by QCM-D and AFM investigations. Out of the phage context, the linear dodecapeptide is reproducibly found to adhere to the gold surface, and by quantitative SPR measurements, high affinity constants (K(eq)~10(6)M(-1), binding energy ~-8 kcal/mol) were determined. We proved that the interactions occurring at gold interface were mainly hydrophobic as a consequence of high frequency of hydrophobic residues in the peptide sequence. Moreover, by CD, molecular dynamics and steered molecular dynamics, we demonstrated that the molecular flexibility only played a minor role in the peptide adsorption. Such noncovalent but specific modification of inorganic surfaces through high affinity biomolecule adsorption represents a general strategy to modulate the functionality of multipurpose metallic surfaces.
authors.affiliation: Interdisciplinary Research Centre on Biomaterials (CRIB), University Federico II, Naples, Italy. causa@unina.it
authors.forename: F
authors.initials: F
authors.lastname: Causa
authors.forename: R
authors.initials: R
authors.lastname: Della Moglie
authors.forename: E
authors.initials: E
authors.lastname: Iaccino
authors.forename: S
authors.initials: S
authors.lastname: Mimmi
authors.forename: D
authors.initials: D
authors.lastname: Marasco
authors.forename: P L
authors.initials: PL
authors.lastname: Scognamiglio
authors.forename: E
authors.initials: E
authors.lastname: Battista
authors.forename: C
authors.initials: C
authors.lastname: Palmieri
authors.forename: C
authors.initials: C
authors.lastname: Cosenza
authors.forename: L
authors.initials: L
authors.lastname: Sanguigno
authors.forename: I
authors.initials: I
authors.lastname: Quinto
authors.forename: G
authors.initials: G
authors.lastname: Scala
authors.forename: P A
authors.initials: PA
authors.lastname: Netti
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D017104:Bacteriophage M13 / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D005818:Genetic Engineering; D006046:Gold / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D019151:Peptide Library; D010455:Peptides; D006046:Gold