title: microRNA-181a is associated with poor prognosis of colorectal cancer.
abstract: miRNAs regulate gene expression at the post-transcriptional level by degradation             of mRNA and translational repression. Recent studies have shown that miR-181a             is dysregulated in several types of cancer; however, the clinical significance             of miR‑181a in colorectal cancer (CRC) remains unclear. We addressed this question             by using quantitative real-time PCR (qRT-PCR) to analyze miR-181a expression in             162 CRC patients. There was no significant difference in miR-181a expression in             normal colon vs. colorectal cancer tissue. The cancer tissue samples were categorized             into a low and high expression group based on miR-181a expression. Comparison             of the clinicopathological factors and prognosis in these two groups showed that             the high expression group had a significantly poorer prognosis than the low expression             group (P=0.011). Multivariate analysis indicated that high miR-181a expression             was an independent significant prognostic factor for CRC. However, there no correlation             was observed between miR-181a expression and clinicopathological parameters. In vitro             analysis revealed that the overexpression of miR-181a repressed the expression             of the tumor suppressor, phosphatase and tensin homolog (PTEN) located on chromosome 10,             at the mRNA level. These data suggest that miR-181a may be a new independent prognostic             factor for CRC patients.
authors.affiliation: Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Nishimura
authors.forename: Rio
authors.initials: R
authors.lastname: Handa
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Tanaka
authors.forename: Kohei
authors.initials: K
authors.lastname: Shibata
authors.forename: Koshi
authors.initials: K
authors.lastname: Mimori
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takemasa
authors.forename: Tsunekazu
authors.initials: T
authors.lastname: Mizushima
authors.forename: Masataka
authors.initials: M
authors.lastname: Ikeda
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Sekimoto
authors.forename: Hideshi
authors.initials: H
authors.lastname: Ishii
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Doki
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
mesh_terms: D000368:Aged; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality; D018572:Disease-Free Survival; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics*; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis / Q000235:genetics*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013534:Survival
chemical_list: C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human