title: Identification of three new N-demethylated and O-demethylated bisbenzylisoquinoline alkaloid metabolites of isoliensinine from dog hepatic microsomes.
abstract: Isoliensinine, a natural phenolic bisbenzyltetrahydroisoquinoline alkaloid, has received considerable attention for its potential biological effects such as antioxidant and anti-HIV activities. From the dog hepatic microsomes of isoliensinine, three new N-demethylated and O-demethylated metabolites, 2-N-desmethyl-isoliensinine (M1), 2'-N-desmethylisoliensinine (M2), and 2'-N-6-O-didesmethylisoliensinine (M3), were identified by high-performance liquid chromatography and data-dependent electrospray ionization tandem mass spectrometry. Possible metabolic pathways for isoliensinine have been proposed. The result should prove very helpful for evaluation of the drug-like properties of isoliensinine and other bisbenzylisoquinoline alkaloids.
authors.affiliation: Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Liping
authors.initials: L
authors.lastname: Li
authors.forename: Huidi
authors.initials: H
authors.lastname: Jiang
authors.forename: Su
authors.initials: S
authors.lastname: Zeng
mesh_terms: D000818:Animals; D044182:Benzylisoquinolines / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D007546:Isoquinolines / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D053858:Metabolic Networks and Pathways; D008862:Microsomes, Liver / Q000737:chemistry* / Q000378:metabolism
chemical_list: D044182:Benzylisoquinolines; D007546:Isoquinolines; C499779:isoliensinine