title: Large-scale chromatin morpho-functional changes during mammalian oocyte growth and differentiation.
abstract: Mammalian oocyte development is characterized by impressive changes in chromatin structure and function within the germinal vesicle (GV). These changes are crucial to confer the oocyte with meiotic and developmental competencies. In cow, oocytes collected from early and middle antral follicles present four patterns of chromatin configuration, from GV0 to GV3, and its progressive condensation has been related to the achievement of developmental potential. During oogenesis, follicular cells are essential for the acquisition of meiotic and developmental competencies and communicate with the oocyte by paracrine and gap junction mediated mechanisms. We recently analyzed the role of gap junction communications (GJC) on chromatin remodeling process during the specific phase of folliculogenesis that coincides with the transcriptional silencing and sequential acquisition of meiotic and developmental capabilities. Our studies demonstrated that GJC between germinal and somatic compartments plays a fundamental role in the regulation of chromatin remodeling and transcription activities during the final oocyte differentiation, throughout cAMP dependent mechanism(s).
authors.affiliation: Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy. alberto.luciano@unimi.it
authors.forename: A M
authors.initials: AM
authors.lastname: Luciano
authors.forename: V
authors.initials: V
authors.lastname: Lodde
authors.forename: F
authors.initials: F
authors.lastname: Franciosi
authors.forename: I
authors.initials: I
authors.lastname: Tessaro
authors.forename: D
authors.initials: D
authors.lastname: Corbani
authors.forename: S
authors.initials: S
authors.lastname: Modina
mesh_terms: D000818:Animals; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D005260:Female; D006801:Humans; D009866:Oogenesis