title: Extinction of aversive memories associated with morphine withdrawal requires ERK-mediated epigenetic regulation of brain-derived neurotrophic factor transcription in the rat ventromedial prefrontal cortex.
abstract: Recent evidence suggests that histone deacetylase (HDAC) inhibitors facilitate extinction of rewarding memory of drug taking. However, little is known about the role of chromatin modification in the extinction of aversive memory of drug withdrawal. In this study, we used conditioned place aversion (CPA), a highly sensitive model for measuring aversive memory of drug withdrawal, to investigate the role of epigenetic regulation of brain-derived neurotrophic factor (BDNF) gene expression in extinction of aversive memory. We found that CPA extinction training induced an increase in recruiting cAMP response element-binding protein (CREB) to and acetylation of histone H3 at the promoters of BDNF exon I transcript and increased BDNF mRNA and protein expression in the ventromedial prefrontal cortex (vmPFC) of acute morphine-dependent rats and that such epigenetic regulation of BDNF gene transcription could be facilitated or diminished by intra-vmPFC infusion of HDAC inhibitor trichostatin A or extracellular signal-regulated kinase (ERK) inhibitor U0126 (1,4-diamino-2,3-dicyano-1,4-bis(methylthio)butadiene) before extinction training. Correspondingly, disruption of the epigenetic regulation of BDNF gene transcription with U0126 or suppression of BDNF signaling with Trk receptor antagonist K252a or BDNF scavenger tyrosine kinase receptor B (TrkB)-Fc blocked extinction of CPA behavior. We also found that extinction training-induced activation of ERK and CREB and extinction of CPA behavior could be potentiated or suppressed by intra-vmPFC infusion of d-cycloserine, a NMDA receptor partial agonist or aminophosphonopentanoic acid, a NMDA receptor antagonist. We conclude that extinction of aversive memory of morphine withdrawal requires epigenetic regulation of BDNF gene transcription in the vmPFC through activation of the ERK-CREB signaling pathway perhaps in a NMDA receptor-dependent manner.
authors.affiliation: State Key Laboratory of Drug Research, Shanghai Institute of Materia Media, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Wei-Sheng
authors.initials: WS
authors.lastname: Wang
authors.forename: Shuo
authors.initials: S
authors.lastname: Kang
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Liu
authors.forename: Mu
authors.initials: M
authors.lastname: Li
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Chuan
authors.initials: C
authors.lastname: Yu
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Chi
authors.forename: Ling
authors.initials: L
authors.lastname: He
authors.forename: Jing-Gen
authors.initials: JG
authors.lastname: Liu
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D001245:Association Learning / Q000187:drug effects / Q000502:physiology*; D001362:Avoidance Learning / Q000502:physiology*; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis / Q000235:genetics*; D002070:Butadienes / Q000494:pharmacology; D042002:Chromatin Assembly and Disassembly / Q000187:drug effects / Q000502:physiology; D003214:Conditioning, Classical / Q000187:drug effects / Q000502:physiology; D017362:Cyclic AMP Response Element-Binding Protein / Q000502:physiology; D063185:Epigenetic Repression / Q000187:drug effects / Q000502:physiology*; D005108:Extinction, Psychological / Q000502:physiology*; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006657:Histones / Q000502:physiology; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009020:Morphine / Q000633:toxicity*; D009021:Morphine Dependence / Q000503:physiopathology*; D009570:Nitriles / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000502:physiology*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D020813:Receptor, trkB / Q000037:antagonists & inhibitors; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology; D012201:Reward; D013375:Substance Withdrawal Syndrome / Q000503:physiopathology*; D014158:Transcription, Genetic
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D002070:Butadienes; C508137:Creb1 protein, rat; D017362:Cyclic AMP Response Element-Binding Protein; D056572:Histone Deacetylase Inhibitors; D006657:Histones; D009570:Nitriles; D047428:Protein Kinase Inhibitors; D016194:Receptors, N-Methyl-D-Aspartate; C113580:U 0126; D009020:Morphine; D020813:Receptor, trkB