title: Nogo-B promotes the epithelial-mesenchymal transition in HeLa cervical             cancer cells via Fibulin-5.
abstract: Cervical cancer is a common malignancy in women worldwide, and the occurrence             of invasion and metastasis is the major cause for most cancer-related deaths.             Epithelial-mesenchymal transition (EMT) has been implicated in the metastasis             of primary tumors and provides molecular mechanisms for cervical cancer metastasis.             We previously reported that Nogo-B mediates cell motility by binding Fibulin-5.             Herein, we show that the increased expression of Nogo-B is correlated with the             degree of cervical cancer metastasis. In HeLa cervical cancer cells, overexpression             of Nogo-B induces the EMT and promotes cell migration and invasion, while inhibiting             cell adhesion. Furthermore, we found that Nogo-B accumulates and co-localizes             with Fibulin-5 in pseudopods, and the downstream effects of overexpression of             Nogo-B on cell motility could be partially abolished by RNA interference against             Fibulin-5. These results suggest that Nogo-B functions as an inducer of cervical             cancer metastasis and that this effect is mediated, at least in part, through             Fibulin-5.
authors.affiliation: State Key Laboratory of Virology, College of Life Sciences, Wuhan University, and Department of Urology, Tongji Hospital, Hubei, Wuhan, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhou
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.forename: Heng
authors.initials: H
authors.lastname: Li
authors.forename: Guoqing
authors.initials: G
authors.lastname: He
authors.forename: Yingle
authors.initials: Y
authors.lastname: Liu
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Qi
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D016326:Extracellular Matrix Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D009185:Myelin Proteins / Q000235:genetics / Q000378:metabolism*; D000070798:Nogo Proteins; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000401:mortality / Q000473:pathology* / Q000628:therapy
chemical_list: D016326:Extracellular Matrix Proteins; C492373:FBLN5 protein, human; D009185:Myelin Proteins; D000070798:Nogo Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C000603199:RTN4 protein, human