title: Multiparametric analyses of human PBMCs loaded ex vivo with a candidate idiotype vaccine for HCV-related lymphoproliferative disorders.
abstract: Hepatitis C virus (HCV) has been identified as one of the major risk factors for type II mixed cryoglobulinemia (MC), during the clinical evolution of chronic hepatitis, which may lead to development of B cell non-Hodgkin's lymphoma (NHL). We have previously shown that the candidate idiotype vaccine, based on the IGKV3-20 light chain protein, is able to induce activation and maturation of circulating antigen presenting cells (APCs) in both HCV-positive and HCV-negative healthy control subjects, with production of Th2-type cytokines. Here, the effect of the recombinant IGKV3-20 protein on human peripheral blood mononuclear cells (PBMCs) from HCV-positive subjects, with known blood levels of cryoglobulins, is shown via gene expression profiling analysis combined to multiparameter flow cytometry and multiplex analyses of cytokines.
authors.affiliation: Laboratory of Molecular Biology and Viral Oncogenesis, National Cancer Institute "Fond. G. Pascale", Naples, Italy.
authors.forename: Annacarmen
authors.initials: A
authors.lastname: Petrizzo
authors.forename: Maria Lina
authors.initials: ML
authors.lastname: Tornesello
authors.forename: Maria
authors.initials: M
authors.lastname: Napolitano
authors.forename: Giovanna
authors.initials: G
authors.lastname: D'Alessio
authors.forename: Angelo
authors.initials: A
authors.lastname: Salomone Megna
authors.forename: Riccardo
authors.initials: R
authors.lastname: Dolcetti
authors.forename: Valli
authors.initials: V
authors.lastname: De Re
authors.forename: Ena
authors.initials: E
authors.lastname: Wang
authors.forename: Franco M
authors.initials: FM
authors.lastname: Marincola
authors.forename: Franco M
authors.initials: FM
authors.lastname: Buonaguro
authors.forename: Luigi
authors.initials: L
authors.lastname: Buonaguro
mesh_terms: D000938:Antigen-Presenting Cells / Q000276:immunology; D003450:Cryoglobulins / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D016174:Hepacivirus / Q000502:physiology*; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D008232:Lymphoproliferative Disorders / Q000097:blood / Q000235:genetics / Q000276:immunology*; D008297:Male; D011994:Recombinant Proteins / Q000276:immunology; D015533:Transcriptional Activation / Q000276:immunology; D014612:Vaccines / Q000276:immunology*
chemical_list: D003450:Cryoglobulins; D016207:Cytokines; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D011994:Recombinant Proteins; D014612:Vaccines