title: An assay for carbohydrate-binding activity of lectins using polyamidoamine dendrimer conjugated with carbohydrates.
abstract: The carbohydrate-binding activity of lectins was examined using polyamidoamine dendrimer conjugated with carbohydrates (sugar-PD). When a C-type lectin, CEL-IV, was mixed with melibiose-PD, large complexes with a diameter of about 1 μm were formed. Changes in the amount of CEL-IV/melibiose-PD complex as an indication of lectin activity were measured sensitively by Rayleigh scattering. The carbohydrate specificity of the lectin was determined on the basis of inhibition of complex-formation by individual carbohydrates. It is suggested that various lectins can also be measured using sugar-PDs to which different carbohydrates are attached.
authors.affiliation: Biomolecular Chemistry Laboratory, Graduate School of Engineering, Nagasaki University, Nagasaki, Japan. hata@nagasaki-u.ac.jp
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Hatakeyama
authors.forename: Ryota
authors.initials: R
authors.lastname: Karino
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Terai
authors.forename: Masanari
authors.initials: M
authors.lastname: Kimura
authors.forename: Hideaki
authors.initials: H
authors.lastname: Unno
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Goda
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D050091:Dendrimers / Q000378:metabolism*; D037181:Lectins, C-Type / Q000378:metabolism*; D011485:Protein Binding; D012616:Sea Cucumbers / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D050091:Dendrimers; D037181:Lectins, C-Type; C104700:PAMAM Starburst