title: Modeling antimicrobial activity of lipopeptides from Bacillus amyloliquefaciens ES-2 against Shewanella putrefaciens in shrimp meat using a response surface method.
abstract: Bacillus amyloliquefaciens ES-2 can produce antimicrobial lipopeptides, including surfactin and fengycin. In this study, the model of antimicrobial activity against Shewanella putrefaciens in shrimp meat by antimicrobial lipopeptides from B. amyloliquefaciens ES-2  was researched by response surface methodology. The results showed that S. putrefaciens had high sensitivity to antimicrobial lipopeptides, which had a MIC of 0.6 mg/ml. A quadratic mathematical model representative of the action of antimicrobial lipopeptides on S. putrefaciens  in shrimp meat was developed as a function of concentration, time, and temperature. A reduction of S. putrefaciens cells of over 2 log cycles could be realized when the temperature was below 5.4°C, the time was over 6 h, and the concentration of the lipopeptides was over 0.3 mg/g.
authors.affiliation: School of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China.
authors.forename: Huanming
authors.initials: H
authors.lastname: Liu
authors.forename: Lijun
authors.initials: L
authors.lastname: Sun
authors.forename: Yaling
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Lei
authors.forename: Defeng
authors.initials: D
authors.lastname: Xu
mesh_terms: D000818:Animals; D000898:Antibiosis; D001407:Bacillus / Q000502:physiology*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D055666:Lipopeptides / Q000378:metabolism / Q000494:pharmacology; D008074:Lipoproteins / Q000378:metabolism / Q000494:pharmacology*; D008433:Mathematics; D008826:Microbial Sensitivity Tests; D008954:Models, Biological; D033561:Penaeidae / Q000382:microbiology*; D010456:Peptides, Cyclic / Q000378:metabolism / Q000494:pharmacology; D012758:Shellfish / Q000382:microbiology*; D020614:Shewanella putrefaciens / Q000254:growth & development*; D013696:Temperature; D013997:Time Factors
chemical_list: D055666:Lipopeptides; D008074:Lipoproteins; D010456:Peptides, Cyclic; C049972:fengycin; C009365:surfactin peptide