title: The levels of hepatocyte growth factor in serum and follicular fluid and the expression of c-Met in granulosa cells in patients with polycystic ovary syndrome.
abstract: OBJECTIVE
  To evaluate the levels of hepatocyte growth factor (HGF) in follicular fluid (FF) and the expression of c-Met in granulosa cells (GCs) with respect to the quality of the oocyte and embryo both in patients with polycystic ovary syndrome (PCOS) and in the normal ovary during controlled ovarian hyperstimulation cycles.
  
  
  METHODS
  Prospective controlled study.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Fifty-nine women undergoing IVF treatment (of whom 21 had PCOS and 38 were in the control group).
  
  
  METHODS
  A total of 168 FF samples were collected at the time of oocyte retrieval. The HGF levels were measured by ELISA, and the mRNA expression of c-Met in GCs was detected by real-time polymerase chain reaction.
  
  
  METHODS
  The predictive values of HGF levels in serum and FF and the mRNA expression of c-Met in GCs for successful fertilization and oocyte-embryo quality.
  
  
  RESULTS
  The levels of HGF in serum and FF and the c-Met expression in GCs were similar between the PCOS and control groups. Granulosa cells of fertilized oocytes (2PN) had a significantly higher level of c-Met expression than that in oocytes that failed to fertilize. The mean HGF level in FF was significantly higher in the grade 1 embryos than in the grades 2-4 embryos.
  
  
  CONCLUSIONS
  This study suggests that HGF/c-Met signaling may be a crucial determinant of fertilization success.
authors.affiliation: Department of Obstetrics and Gynecology, Dokuz Eylül University Medical School Hospital, İzmir, Turkey. Electronic address: nurbuldanli@yahoo.com.
authors.forename: Nur
authors.initials: N
authors.lastname: Şahin
authors.affiliation: Department of Medical Biology and Genetics, Dokuz Eylül University Medical School Hospital, İzmir, Turkey.
authors.forename: Aslı
authors.initials: A
authors.lastname: Toylu
authors.affiliation: Department of Obstetrics and Gynecology, Dokuz Eylül University Medical School Hospital, İzmir, Turkey.
authors.forename: Bülent
authors.initials: B
authors.lastname: Gülekli
authors.affiliation: Department of Obstetrics and Gynecology, Dokuz Eylül University Medical School Hospital, İzmir, Turkey.
authors.forename: Erbil
authors.initials: E
authors.lastname: Doğan
authors.affiliation: Department of Obstetrics and Gynecology, Dokuz Eylül University Medical School Hospital, İzmir, Turkey.
authors.forename: Müge
authors.initials: M
authors.lastname: Kovali
authors.affiliation: Department of Medical Biology and Genetics, Dokuz Eylül University Medical School Hospital, İzmir, Turkey.
authors.forename: Neşe
authors.initials: N
authors.lastname: Atabey
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D006107:Granulosa Cells / Q000378:metabolism* / Q000473:pathology; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D054315:Oocyte Retrieval; D009865:Oocytes / Q000502:physiology; D010062:Ovulation Induction; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000473:pathology; D011446:Prospective Studies; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism*; D044967:Serum / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met