title: Impacts of the N-terminal fragment analog of human parathyroid hormone on structure, composition and biomechanics of bone.
abstract: Osteoporosis is a skeletal disease characterized by low bone mass and microarchitectural deterioration of bone tissue, and it is a serious threat to human lives. We previously showed that the N-terminal peptide analog of human parathyroid hormone (Pro-Pro-PTH(1-34)) enhanced plasma calcium concentration. In this paper, we study the impact of PTH N-terminal fragment analog on the structure, component, and mechanical properties of the rat bones. Daily subcutaneous injections of Pro-Pro-hPTH (1-34) induces 26.5-32.8% increase in femur bone mineral density (BMD), 23.0-34.2% decrease the marrow cavity or increase in trabecular bone area. The peptide also increases 16.0-59.5%, 28.8-48.2% and 14.0-17.8% of bone components of calcium, phosphorus and collagen, respectively. In terms of mechanic properties, administration of the peptide elevates the bone rigidity by 45.4-76.6%, decreases the flexibility by 23.0-31.6%, and improves modulus of elasticity by 32.8-63.4%. The results suggest that Pro-Pro-hPTH (1-34) has a positive effect on bone growth and strength, and possesses anti-fracture capability, thus a potential candidate for the application for the treatment of osteoporosis.
authors.affiliation: Branch of Marine Biopharmaceutical, College of Food Science and Technology, Shanghai Ocean University, China. cxwang@shou.edu.cn
authors.forename: Wang
authors.initials: W
authors.lastname: Chunxiao
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yu
authors.forename: Liu
authors.initials: L
authors.lastname: Wentao
authors.forename: Liu
authors.initials: L
authors.lastname: Jingjing
authors.forename: Ye
authors.initials: Y
authors.lastname: Jiahui
authors.forename: Chen
authors.initials: C
authors.lastname: Qingmei
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D002118:Calcium / Q000097:blood; D003094:Collagen / Q000378:metabolism; D055119:Elastic Modulus; D005260:Female; D005269:Femur / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D010052:Ovariectomy; D010281:Parathyroid Hormone / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D010758:Phosphorus / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D050071:Bone Density Conservation Agents; D010281:Parathyroid Hormone; D010446:Peptide Fragments; D011994:Recombinant Proteins; C040288:amino-terminal parathyroid hormone; D010758:Phosphorus; D003094:Collagen; D000469:Alkaline Phosphatase; D002118:Calcium