title: Hydrophilic interaction/weak cation-exchange mixed-mode chromatography for chitooligosaccharides separation.
abstract: A hydrophilic interaction/weak cation-exchange (HILIC/WCX) mixed-mode chromatography was developed for separation of chitooligosaccharides (COS) using a weak cation-exchange column. For better understanding of the retention characteristics of the column, organic solvent content effect was investigated. WCX mode, HILIC mode, and mixed-mode could be performed on this column according to investigating results. COS were separated on the column in mixed-mode. The usage of volatile buffer salt made it favorable for evaporative light-scattering detector (ELSD). Mild elution conditions were economical and environment-friendly relative to other conditions used in early reports. This method may offer a possibility for large-scale COS preparation.
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, 457 Zhongshan Road, Dalian 116023, China.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Dong
authors.forename: Aijin
authors.initials: A
authors.lastname: Shen
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Gou
authors.forename: Deliang
authors.initials: D
authors.lastname: Chen
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D002412:Cations / Q000737:chemistry; D002852:Chromatography, Ion Exchange; D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D009844:Oligosaccharides / Q000302:isolation & purification*
chemical_list: D002412:Cations; D009844:Oligosaccharides