title: Crosslinked redox polymer enzyme electrodes containing carbon nanotubes for high and stable glucose oxidation current.
abstract: Co-immobilisation approaches for preparation of glucose-oxidising films of [Os(2,2'-bipyridine)(2)(poly-vinylimidazole)(10)Cl] and glucose oxidase on glassy carbon electrodes are compared. Electrodes prepared by crosslinking using glutaraldehyde vapour, without and with a NaBH(4) reduction, provide higher glucose oxidation current than those prepared using a well-established diepoxide method. Addition of multi walled carbon nanotubes to the film deposition solutions produces an enhanced glucose oxidation current density of 5 mA cm(-2) at 0.35 V vs. Ag/AgCl, whilst improving the operational stability of the current signal. Carbon nanotube, glutaraldehyde vapour crosslinked, films on electrodes, reduced by NaBH(4), retain 77% of initial catalytic current over 24 hours of continuous amperometric testing in a 37 °C, 50 mM phosphate buffer solution containing 150 mM NaCl and 100 mM glucose. Potential application of this approach to implantable enzymatic biofuel cells is demonstrated by production of glucose oxidation currents, under pseudo-physiological conditions, using mediating films with lower redox potentials.
authors.affiliation: School of Chemistry & Ryan Institute, National University of Ireland Galway, Ireland.
authors.forename: Domhnall
authors.initials: D
authors.lastname: MacAodha
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Ferrer
authors.forename: Peter Ó
authors.initials: PÓ
authors.lastname: Conghaile
authors.forename: Paul
authors.initials: P
authors.lastname: Kavanagh
authors.forename: Dónal
authors.initials: D
authors.lastname: Leech
mesh_terms: D004566:Electrodes; D005947:Glucose / Q000737:chemistry* / Q000378:metabolism; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D005976:Glutaral / Q000737:chemistry / Q000378:metabolism; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry* / Q000378:metabolism; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism
chemical_list: D037742:Nanotubes, Carbon; D009942:Organometallic Compounds; D011108:Polymers; D005949:Glucose Oxidase; D005947:Glucose; D005976:Glutaral