title: [The mechanisms of inhibitory effect of adenovirus-mediated wild-type PTEN gene on the proliferation in activated hepatic stellate cells in vitro].
abstract: OBJECTIVE
  Using an adenoviral vector, the wild-type PTEN gene was transduced into activated hepatic stellate cell (HSC) cultured in vitro and cell cycle markers and were detect. Thereby, the potential mechanisms of inhibitory effect of the wild-type PTEN overexpression on the proliferation in activated HSC was investigated.
  
  
  METHODS
  The wild type PTEN gene was transduced into activated HSC (HSC-T6 ) cultured in vitro mediated by adenoviral vector. PTEN expression in HSC was measured by Western blot and Real-time fluorescent quantitation PCR. Flow cytometry (FCM) was then used to detect cell cycle phase of activated HSC. And the expressions of cyclinD1 and cyclin dependent kinase 4 (CDK4) in HSC were determined by Western blot.
  
  
  RESULTS
  The data showed that exogenous wild type PTEN gene was successfully transduced and expressed in activated HSC cultured in vitro. The over-expression of wild type PTEN resulted in the increased number of HSC at G0/G1 phase ( P less than 0.01), and the number of HSC at S phase and G2/M phase were decreased significantly, P less than 0.01. Furthermore, there were decreased cyclinD1 and CDK4 expression in HSC infected with Ad-PTEN, P less than 0.01.
  
  
  CONCLUSIONS
  The over-expression of wild type PTEN inhibit transition of activated HSC in vitro from G1 to S phase and arrest cell cycle of them at G0/G1 phase via the down-regulated expressions of cyclinD1 and CDK4, and then inhibit HSC proliferation.
authors.affiliation: Department of Gastroenterology, the Affiliated Hospital of Hebei United University, Tangshan, China.
authors.forename: Li-sen
authors.initials: LS
authors.lastname: Hao
authors.forename: Xiao-lan
authors.initials: XL
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Li-wen
authors.initials: LW
authors.lastname: Li
authors.forename: Yan-bo
authors.initials: YB
authors.lastname: Mo
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Yao
authors.forename: Hui-qing
authors.initials: HQ
authors.lastname: Jiang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1 / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism*; D005822:Genetic Vectors; D055166:Hepatic Stellate Cells / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000494:pharmacology*; D051381:Rats; D014162:Transfection
chemical_list: C530106:Ccnd1 protein, rat; D019938:Cyclin D1; C495903:Cdk4 protein, rat; D051358:Cyclin-Dependent Kinase 4; D051059:PTEN Phosphohydrolase