title: Complete nucleotide sequence of plasmid pND6-2 from Pseudomonas putida ND6 and characterization of conjugative genes.
abstract: Pseudomonas putida ND6 was shown to be capable of growth using naphthalene as sole carbon and energy sources. One plasmid from this strain, pND6-1, which was associated with the metabolism of naphthalene, was characterized; and the complete nucleotide sequence (101,858bp) and annotation of the plasmid were reported previously. In this paper, another plasmid (117,003bp) from P. putida ND6, pND6-2, was sequenced and 136 putative coding sequences (CDSs) were annotated. Among them, 9 CDSs were predicted to be involved in replication and partition of the plasmid, 32 CDSs encoded proteins associated with plasmid conjugative transfer, 2 CDSs encoded transcriptional regulators, 9 CDSs were necessary for DNA-associated function (including metabolism, recombination and repair), and 5 CDSs encoded proteins associated with other functions. The filter matting experiment indicated that pND6-2 is a helper plasmid, which could assist the naphthalene catabolic plasmid pND6-1 without any conjugative genes being transferred from P. putida ND6 to Escherichia coli AD256.
authors.affiliation: Tianjin Key Laboratory of Biomarkers for Occupational and Environmental Hazard, Logistics University of Chinese People's Armed Police Forces, Tianjin 300162, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.forename: Huabing
authors.initials: H
authors.lastname: Zhao
authors.forename: Yaxiao
authors.initials: Y
authors.lastname: Li
authors.forename: Shumin
authors.initials: S
authors.lastname: Niu
authors.forename: Baoli
authors.initials: B
authors.lastname: Cai
mesh_terms: D003227:Conjugation, Genetic; D004260:DNA Repair / Q000235:genetics; D004261:DNA Replication; D023061:Gene Order; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D016958:Pseudomonas putida / Q000235:genetics*; D011995:Recombination, Genetic