title: Histochemical analysis of heme degradation enzymes.
abstract: Histochemical analysis can be used to study the cellular localization, expression, and regulation of enzymes responsible for catabolism of the heme molecule. This unit describes protocols for assessing the expression and regulation of specific HO-1 and HO-2 mRNAs by in situ hybridization using digoxigenin-tagged probes. Immunohistochemistry is used to visualize specific cellular sites of HO-1 and HO-2 protein expression.
authors.affiliation: ArQule, Inc., Waltham, Massachusetts, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Ewing
mesh_terms: D000818:Animals; D015971:Gene Expression Regulation, Enzymologic; D006418:Heme / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D017403:In Situ Hybridization / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D006418:Heme; D006419:Heme Oxygenase (Decyclizing); D051547:Heme Oxygenase-1; C105245:heme oxygenase-2