title: Protection by a recombinant Mycobacterium bovis Bacillus Calmette-Guerin vaccine expressing Shiga toxin 2 B subunit against Shiga toxin-producing Escherichia coli in mice.
abstract: We have developed a novel vaccine against Shiga toxin (Stx)-producing Escherichia coli (STEC) infection using a recombinant Mycobacterium bovis BCG (rBCG) system. Two intraperitoneal vaccinations with rBCG expressing the Stx2 B subunit (Stx2B) resulted in an increase of protective serum IgG and mucosal IgA responses to Stx2B in BALB/c mice. When orally challenged with 10(3) CFU of STEC strain B2F1 (O91: H21), the immunized mice survived statistically significantly longer than the nonvaccinated mice. We suggest that intraperitoneal immunization with rBCG expressing Stx2B would be a potential vaccine strategy for STEC.
authors.affiliation: Department of Bacteriology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. junfujii@bact.med.kyushu-u.ac.jp
authors.forename: Jun
authors.initials: J
authors.lastname: Fujii
authors.forename: Mariko
authors.initials: M
authors.lastname: Naito
authors.forename: Takashi
authors.initials: T
authors.lastname: Yutsudo
authors.forename: Sohkichi
authors.initials: S
authors.lastname: Matsumoto
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Heatherly
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Tom
authors.initials: T
authors.lastname: Obrig
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000235:genetics* / Q000276:immunology; D004195:Disease Models, Animal; D004337:Drug Carriers; D004927:Escherichia coli Infections / Q000517:prevention & control; D022361:Escherichia coli Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D005260:Female; D005822:Genetic Vectors; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C; D022641:Shiga Toxin 2 / Q000235:genetics / Q000276:immunology*; D054323:Shiga-Toxigenic Escherichia coli / Q000235:genetics / Q000276:immunology*; D016019:Survival Analysis; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D001500:BCG Vaccine; D004337:Drug Carriers; D022361:Escherichia coli Vaccines; D022641:Shiga Toxin 2; D014614:Vaccines, Synthetic; C450841:shiga toxin 2 B subunit