title: Use of abundance ratios of somatic coliphages and bacteriophages of Bacteroides thetaiotaomicron GA17 for microbial source identification.
abstract: Water contaminated with human faeces is a risk to human health and management of water bodies can be improved by determining the sources of faecal pollution. Field studies show that existing methods are insufficient and that different markers are required. This study proposes the combined use of two microbial indicators, the concentrations of which are presented as ratios. This provides a more reliable approach to identifying faecal sources as it avoids variation due to treatment or ageing of the contamination. Among other indicators, bacteriophages have been proposed as rapid and cheap indicators of faecal pollution. Samples analysed in this study were derived from wastewater treatment plants (raw sewage, secondary and tertiary effluents and raw sewage sludge) river water, seawater and animal related wastewater. The abundance ratios of faecal coliforms and Bacteroides phages, either strain RYC2056 (non-specific for faecal origin) or strain GA17 (specific for human pollution), and among somatic coliphages and phages infecting both Bacteroides strains, were evaluated. The results indicate that the ratio of somatic coliphages and phages infecting Bacteroides strain GA17, which is specific to human faecal sources, provides a robust method for discriminating samples, even those presenting different levels and ages of pollution, and allows samples polluted with human faeces to be distinguished from those containing animal faecal pollution. This method allows the generation of numerical data that can be further applied to numerical methods for faecal pollution discrimination.
authors.affiliation: Department of Microbiology, Faculty of Biology, University of Barcelona, Barcelona, Spain.
authors.forename: Maite
authors.initials: M
authors.lastname: Muniesa
authors.forename: Francisco
authors.initials: F
authors.lastname: Lucena
authors.forename: Anicet R
authors.initials: AR
authors.lastname: Blanch
authors.forename: Andrey
authors.initials: A
authors.lastname: Payán
authors.forename: Juan
authors.initials: J
authors.lastname: Jofre
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000302:isolation & purification*; D001439:Bacteroides / Q000821:virology*; D003090:Coliphages / Q000302:isolation & purification*; D004784:Environmental Monitoring / Q000379:methods; D005243:Feces / Q000382:microbiology*; D006801:Humans; D045483:Rivers / Q000382:microbiology; D012722:Sewage; D013030:Spain; D062065:Wastewater / Q000382:microbiology; D014871:Water Microbiology; D014876:Water Pollution / Q000032:analysis*
chemical_list: D012722:Sewage; D062065:Wastewater