title: Immunospecific targeting of CD45 expressing lymphoid cells: towards improved detection agents of the sentinel lymph node.
abstract: This study was designed to demonstrate the potential of small nanoparticulate lymphotropic contrast agents designed to bind with high affinity to lymphoid cells overexpressing the CD45 antigen. To this end, small gold nanoparticles used as model were conjugated to anti-CD45 antibodies and injected in mice in the dorsal toe of the fore/hind paw. Chemical analysis demonstrated rapid uptake and transport of the nanoparticles in the lymphatic as well as significant retention of the nanoparticles with high binding affinity to lymphoid cells in the popliteal and axillary lymph nodes in comparison to non-targeted nanoparticles.
authors.affiliation: Ian Wark Research Institute, University of South Australia, Mawson Lakes Campus, Mawson Lakes, Adelaide, SA 5095, Australia.
authors.forename: Tianqing
authors.initials: T
authors.lastname: Liu
authors.forename: Aidan
authors.initials: A
authors.lastname: Cousins
authors.forename: Chia-Chi
authors.initials: CC
authors.lastname: Chien
authors.forename: Ivan
authors.initials: I
authors.lastname: Kempson
authors.forename: Sarah
authors.initials: S
authors.lastname: Thompson
authors.forename: Yeukuang
authors.initials: Y
authors.lastname: Hwu
authors.forename: Benjamin
authors.initials: B
authors.lastname: Thierry
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry / Q000378:metabolism; D018796:Immunoconjugates / Q000737:chemistry; D017493:Leukocyte Common Antigens / Q000737:chemistry / Q000378:metabolism*; D008198:Lymph Nodes / Q000378:metabolism* / Q000473:pathology; D008214:Lymphocytes / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding / Q000276:immunology
chemical_list: D003287:Contrast Media; D018796:Immunoconjugates; D017493:Leukocyte Common Antigens