title: A PCR based survey of Babesia ovata in cattle from various Asian, African and South American countries.
abstract: Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B. ovata infection in Japan, Mongolia, and Thailand.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yoshinari
authors.forename: Thillaiampalam
authors.initials: T
authors.lastname: Sivakumar
authors.forename: Masahito
authors.initials: M
authors.lastname: Asada
authors.forename: Badgar
authors.initials: B
authors.lastname: Battsetseg
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Huang
authors.forename: Dinh Thi Bich
authors.initials: DT
authors.lastname: Lan
authors.forename: Tawin
authors.initials: T
authors.lastname: Inpankaew
authors.forename: Adrian P
authors.initials: AP
authors.lastname: Ybañez
authors.forename: Andy
authors.initials: A
authors.lastname: Alhassan
authors.forename: Oriel M M
authors.initials: OM
authors.lastname: Thekisoe
authors.forename: Alan Caine Costa
authors.initials: AC
authors.lastname: De Macedo
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
authors.forename: Ikuo
authors.initials: I
authors.lastname: Igarashi
authors.forename: Naoaki
authors.initials: N
authors.lastname: Yokoyama
mesh_terms: D000349:Africa / Q000453:epidemiology; D000818:Animals; D001208:Asia / Q000453:epidemiology; D001403:Babesia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D001404:Babesiosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D002417:Cattle; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D016036:Seroepidemiologic Studies; D013020:South America / Q000453:epidemiology