title: Antibody-linked spherical nucleic acids for cellular targeting.
abstract: Spherical nucleic acid (SNA) constructs are promising new single entity gene regulation materials capable of both cellular transfection and gene knockdown, but thus far are promiscuous structures, exhibiting excellent genetic but little cellular selectivity. In this communication, we describe a strategy to impart targeting capabilities to these constructs through noncovalent functionalization with a complementary antibody-DNA conjugate. As a proof-of-concept, we designed HER2-targeting SNAs and demonstrated that such structures exhibit cell type selectivity in terms of their uptake, and significantly greater gene knockdown in cells overexpressing the target antigen as compared to the analogous antibody-free and off-target materials.
authors.affiliation: Department of Chemistry and the International Institute for Nanotechnology, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhang
authors.forename: Liangliang
authors.initials: L
authors.lastname: Hao
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Hurst
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D045744:Cell Line, Tumor; D016373:DNA, Antisense / Q000008:administration & dosage* / Q000737:chemistry / Q000235:genetics / Q000493:pharmacokinetics; D055785:Gene Knockdown Techniques; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry / Q000276:immunology*; D008958:Models, Molecular; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D016373:DNA, Antisense; D018796:Immunoconjugates; D018719:Erb-b2 Receptor Tyrosine Kinases