title: Modulation of inflammation by mesenchymal stem cell transplantation in peritoneal dialysis in rats.
abstract: OBJECTIVE
  The purpose of this study was to determine the effect of mesenchymal stem cell (MSC) transplantation on the peritoneal morphology and inflammation markers in rat models of peritoneal dialysis (PD).
  
  
  METHODS
  Wistar albino rats were divided into two groups: control (C) (n = 8) and experimental groups (n = 50). PD solution was given to the experimental group during 6 weeks. Then, experimental group was divided into three groups as PD, MSC, and placebo (P) groups. MSC group was treated with MSC (1.5 × 10(6) cells/kg) and P group was treated with phosphate buffer solution via intraperitoneal injection. Evaluation was performed to C and PD groups at the end of 6 weeks and to MSC and P groups at second and third week of the treatment (MSC-2, P-2, MSC-3, and P-3 groups).
  
  
  RESULTS
  The submesothelial area was significantly thickened in PD and P groups compared to C and MSC groups. Peritoneal fibrosis was seen in P-3 group but not in MSC group. There were no significant differences between the MSC-3 and C groups according to morphological findings. Levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were significantly increased in MSC-2 group compared to the other groups (p-values ranged from 0.0001 to 0.04). TNF-α and IL-6 levels in MSC-3 and P-3 groups were lower than PD and C groups (p < 0.0001 for TNF-α and p = 0.0001-0.002 for IL-6).
  
  
  CONCLUSIONS
  Giving MSC may protect the peritoneal membrane from the deleterious effect of PD and extend the life of the peritoneal membrane. Our study is the first on this issue and more detailed studies are needed.
authors.affiliation: Department of Pediatric Nephrology, Erciyes University Faculty of Medicine, Kayseri, Turkey. stulpar76@yahoo.com
authors.forename: Sebahat
authors.initials: S
authors.lastname: Tülpar
authors.forename: M Hakan
authors.initials: MH
authors.lastname: Poyrazoğlu
authors.forename: Hatice
authors.initials: H
authors.lastname: Özbilge
authors.forename: Funda
authors.initials: F
authors.lastname: Baştuğ
authors.forename: Zübeyde
authors.initials: Z
authors.lastname: Gündüz
authors.forename: Yasemin A
authors.initials: YA
authors.lastname: Torun
authors.forename: Esma G
authors.initials: EG
authors.lastname: Kaya
authors.forename: Hülya
authors.initials: H
authors.lastname: Akgün
authors.forename: Ismail
authors.initials: I
authors.lastname: Dursun
authors.forename: Ruhan
authors.initials: R
authors.lastname: Düşünsel
mesh_terms: D000818:Animals; D007249:Inflammation / Q000209:etiology / Q000517:prevention & control*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D010530:Peritoneal Dialysis / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar