title: Aeromonas hydrophila subsp. dhakensis--a causative agent of gastroenteritis imported into the Czech Republic.
abstract: Out of the twenty-one A. hydrophila complex isolates obtained during a routine examination of human diarrhoeal faeces, two A. hydrophila subsp. dhakensis isolates (P1097 = CCM 7329 and P1165) were successfully identified by ribotyping. The correct taxonomic position of the A. hydrophila subsp. dhakensis CCM 7329 was verified by cpn60 sequencing (GeneBank accession number HM536193). The remaining A. hydrophila complex isolates were identified as A. hydrophila subsp. hydrophila. The ability of biochemical tests and fatty acid methyl ester analysis to reliably discern both A. hydrophila subsp. dhakensis and A. hydrophila subsp. hydrophila was limited. In contrast to the A. hydrophila subsp. hydrophila, the faecal isolates of A. hydrophila subsp. dhakensis did not produce acid from arbutin. When compared in a two-dimensional plot, the A. hydrophila subsp. dhakensis faecal isolates contained higher amounts of the two minor fatty acids C(13:0) and C(17:1) ω8c than the A. hydrophila subsp. hydrophila reference strain. This is the first detected occurrence of the less frequent A. hydrophila subsp. dhakensis in our region and ribotyping was proved as a suitable method for the identification of A. hydrophila subsp. dhakensis.
authors.affiliation: Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Brno, Czech Republic. ivo@sci.muni.cz
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedláček
authors.forename: Eva
authors.initials: E
authors.lastname: Krejčí
authors.forename: Anna
authors.initials: A
authors.lastname: Andělová
authors.forename: Michaela
authors.initials: M
authors.lastname: Sedláčková
authors.forename: Iva
authors.initials: I
authors.lastname: Porazilová
authors.forename: Pavla
authors.initials: P
authors.lastname: Holochová
mesh_terms: D016980:Aeromonas hydrophila / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics*; D018835:Chaperonin 10 / Q000235:genetics*; D018153:Czech Republic / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D021521:Ribotyping; D017422:Sequence Analysis, DNA
chemical_list: D001426:Bacterial Proteins; D018835:Chaperonin 10; D005227:Fatty Acids