title: Near-surface oxidized sulfur modifications and self-assembly of thiol-modified aptamer on Au thin film substrates influenced by piranha treatment.
abstract: Self-assembly of thiol-modified oligonucleotides on Au films has great importance for biosensor applications. Prior to the self-assembly, a piranha treatment (PT) is commonly used to clean the Au surface. Here we report that near-surface oxidized sulfur modifications on Au thin films by PT for longer than 60 s have serious effects on the self-assembled monolayer (SAM) formation of thiol-modified single-stranded thrombin binding aptamer (s-TBA), and a PT time of 10-30 s is optimal for s-TBA SAM formation. These results have important implication to SAM formation of biomolecules, especially for the thiol-modified ones where a careful consideration of this key step could significantly enhance the SAM formation and biosensor performance.
authors.affiliation: WATLab and Department of Chemistry, University of Waterloo , Waterloo, Ontario N2L3G1, Canada.
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Thomas
authors.forename: Liyan
authors.initials: L
authors.lastname: Zhao
authors.forename: Kejian
authors.initials: K
authors.lastname: Ding
authors.forename: Nina F
authors.initials: NF
authors.lastname: Heinig
authors.forename: Kam Tong
authors.initials: KT
authors.lastname: Leung
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006358:Hot Temperature; D008422:Materials Testing; D008567:Membranes, Artificial; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000737:chemistry*; D013455:Sulfur / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial; D013438:Sulfhydryl Compounds; D013455:Sulfur; D006046:Gold