title: Interleukin-15 activated bovine natural killer cells express CD69 and produce interferon-γ.
abstract: Interleukin (IL)-15 is an essential cytokine in natural killer (NK) cell development and survival. In humans, IL-15 shows overlapping properties with IL-2 due to partly shared receptors and signal transduction and both cytokines synergize equally well with IL-12 in the induction of interferon (IFN)-γ production from NK cells. Bovine NK cells however, have been reported to produce less IFN-γ after in vitro IL-12 stimulation when exposed to human IL-15 in comparison to bovine IL-2. We therefore wanted to determine if homologous IL-15 is needed for adequate stimulation of bovine NK cells. Biologically active recombinant bovine IL-15 (rbIL-15) produced in mammalian cells by the use of a modified expression vector stimulated NK cells to a dose-dependent IFN-γ production in the presence of IL-12. In contrast to earlier findings, we also detected potent IFN-γ production from bovine NK cells stimulated by human IL-15 and IL-12. Finally, we describe a monoclonal antibody recognizing bovine CD69 and show the expression of this early activation marker on bovine NK cells ex vivo and following rbIL-15 stimulation.
authors.affiliation: Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146 Dep., N-0033, Oslo, Norway. hege.lund@nvh.no
authors.forename: Hege
authors.initials: H
authors.lastname: Lund
authors.forename: Preben
authors.initials: P
authors.lastname: Boysen
authors.forename: Gregg A
authors.initials: GA
authors.lastname: Dean
authors.forename: William C
authors.initials: WC
authors.lastname: Davis
authors.forename: Kun Taek
authors.initials: KT
authors.lastname: Park
authors.forename: Anne K
authors.initials: AK
authors.lastname: Storset
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D002417:Cattle / Q000097:blood / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D019409:Interleukin-15 / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D037181:Lectins, C-Type / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008297:Male; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D000099192:CD69 Antigens
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D007371:Interferon-gamma; D019409:Interleukin-15; D037181:Lectins, C-Type; D011994:Recombinant Proteins; D000099192:CD69 Antigens