title: Identification of an O-linked repetitive glycan chain of the polar flagellum flagellin of Azospirillum brasilense Sp7.
abstract: This is the first report to have identified an O-linked repetitive glycan in bacterial flagellin, a structural protein of the flagellum. Studies by sugar analysis, Smith degradation, (1)H and (13)C NMR spectroscopy, and mass spectrometry showed that the glycan chains of the polar flagellum flagellin of the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp7 are represented by a polysaccharide with a molecular mass of 7.7 kDa, which has a branched tetrasaccharide repeating unit of the following structure:
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia.
authors.forename: Alexei Ye
authors.initials: AY
authors.lastname: Belyakov
authors.forename: Gennady L
authors.initials: GL
authors.lastname: Burygin
authors.forename: Nikolai P
authors.initials: NP
authors.lastname: Arbatsky
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Nikolai Yu
authors.initials: NY
authors.lastname: Selivanov
authors.forename: Larisa Yu
authors.initials: LY
authors.lastname: Matora
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
authors.forename: Sergei Yu
authors.initials: SY
authors.lastname: Shchyogolev
mesh_terms: D015806:Azospirillum brasilense / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D005407:Flagella / Q000737:chemistry* / Q000378:metabolism; D005408:Flagellin / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D005408:Flagellin