title: Uptake of fluorescent nano beads into BY2-cells involves clathrin-dependent and clathrin-independent endocytosis.
abstract: To follow endocytosis in BY-2 cells we made use of fluorescent nano beads. Beads with 20nm in diameter were internalised rapidly and accumulated partially in compartments also labelled by the endocytic marker FM4-64. Studies in BY-2 cells and protoplasts revealed that larger beads (100nm) were excluded from uptake into turgescent and plasmolysed cells while protoplasts were able to internalise beads with a diameter of up to 1000nm. Endocytosis of beads was only partially inhibited by the clathrin-specific inhibitor Ikarugamycin and strongly blocked by wortmannin. These results imply that uptake of beads involves clathrin-dependent and clathrin-independent endocytic mechanisms and supports the hypothesis that clathrin-independent endocytosis plays a general role in plants.
authors.affiliation: Plant Cell Biology, Technische Universität Darmstadt, Schnittspahnstr. 3-5, 64287 Darmstadt, Germany.
authors.forename: Vera
authors.initials: V
authors.lastname: Bandmann
authors.forename: Jasmin Daniela
authors.initials: JD
authors.lastname: Müller
authors.forename: Tim
authors.initials: T
authors.lastname: Köhler
authors.forename: Ulrike
authors.initials: U
authors.lastname: Homann
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002966:Clathrin / Q000378:metabolism*; D004705:Endocytosis; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D010940:Plant Proteins / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology*
chemical_list: D002966:Clathrin; D005456:Fluorescent Dyes; D010940:Plant Proteins