title: A novel method of diffraction imaging flow cytometry for sizing microspheres.
abstract: We report a novel method of diffraction imaging flow cytometry to measure and analyze size distribution of microspheres. An automated and robust image processing software based on the short-time-Fourier-transform algorithm has been developed to analyze the characteristic and spatially varying oscillations of side scatters recorded as a diffraction image. Our results demonstrate that the new method allows accurate and rapid determination of single microspheres' diameters ranging from 1 to 100 μm. The capacity for analysis of light scattering by two-sphere aggregates has been demonstrated but analytical tools for characterization of aggregates by multiple microspheres remain to be developed.
authors.affiliation: Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Sa
authors.initials: S
authors.lastname: Yu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Marina S
authors.initials: MS
authors.lastname: Moran
authors.forename: Jun Q
authors.initials: JQ
authors.lastname: Lu
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Feng
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Hu
mesh_terms: D000465:Algorithms; D001185:Artificial Intelligence; D005434:Flow Cytometry / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008863:Microspheres; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D010316:Particle Size; D010363:Pattern Recognition, Automated / Q000379:methods*