title: A new bacterial disease on Mandevilla sanderi, caused by Pseudomonas savastanoi: lessons learned for bacterial diversity studies.
abstract: Leaf lesions of Mandevilla sanderi were shown to be caused by Pseudomonas savastanoi. While BOX fingerprints were similar for P. savastanoi isolates from different host plants, plasmid restriction patterns and sequencing of plasmid-located pathogenicity determinants revealed that Mandevilla isolates contained similar plasmids distinct from those of other isolates. A repA-based detection method was established.
authors.affiliation: Julius Kühn-Institut, Federal Research Centre for Cultivated Plants (JKI), Institute for Epidemiology and Pathogen Diagnostics, Braunschweig, Germany.
authors.forename: Namis
authors.initials: N
authors.lastname: Eltlbany
authors.forename: Zsa-Zsa
authors.initials: ZZ
authors.lastname: Prokscha
authors.forename: M Pilar
authors.initials: MP
authors.lastname: Castañeda-Ojeda
authors.forename: Ellen
authors.initials: E
authors.lastname: Krögerrecklenfort
authors.forename: Holger
authors.initials: H
authors.lastname: Heuer
authors.forename: Walter
authors.initials: W
authors.lastname: Wohanka
authors.forename: Cayo
authors.initials: C
authors.lastname: Ramos
authors.forename: Kornelia
authors.initials: K
authors.lastname: Smalla
mesh_terms: D029062:Apocynaceae / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000379:methods; D004265:DNA Helicases / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D010957:Plasmids / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D011549:Pseudomonas / Q000302:isolation & purification* / Q000472:pathogenicity*; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D015534:Trans-Activators; D037521:Virulence Factors; C045264:replication initiator protein; D004265:DNA Helicases