title: [Study on the spatial and temporal expression of beta-AS gene of Glycyrriza uralensis].
abstract: OBJECTIVE
  To reveal the temporal and spatial specificity of the expression of beta-AS gene of Glycyrriza uralensis.
  
  
  METHODS
  Used PCR to obtain the cDNA of beta-AS gene of Glycyrriza uralensis at different time and from different part and Gel-Pro to carry out the densitometric analysis of the electrophoretic band, then calculate the relative expression.
  
  
  RESULTS
  The spatial specificity experiment showed that beta-AS gene didn't express in the overground part of Glycyrriza uralensis,while in the underground part,the expression of beta-AS in root tip was higher than that of rootstock. And the temporal specificity experiment showed that the expression of beta-AS gene of Glycyrriza uralensis could be divided into 4 stages. From December to February, the expression of beta-AS gene was under the detection limit. From March to May, beta-AS gene began to express. From May to September,the expression of beta-AS gene kept at a high level. And in October and November the expression of beta-AS gene began to decrease.
  
  
  CONCLUSIONS
  When the beta-AS gene of Glycyrriza uralensis is researched, the root tip is the suitable plant material and May, June, August and September are the right acquisition time.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China. liuyliwd@vip.sina.com.cn
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Liu
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D029882:Glycyrrhiza uralensis / Q000201:enzymology* / Q000235:genetics; D019695:Glycyrrhizic Acid / Q000378:metabolism; D019751:Intramolecular Transferases / Q000235:genetics / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000201:enzymology / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D010946:Plants, Medicinal / Q000201:enzymology* / Q000235:genetics; D012621:Seasons; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D019695:Glycyrrhizic Acid; D019751:Intramolecular Transferases; C039924:2,3-oxidosqualene-beta-amyrin-cyclase