title: Sentinel lymph nodes fluorescence detection and imaging using Patent Blue V bound to human serum albumin.
abstract: Patent Blue V (PBV), a dye used clinically for sentinel lymph node detection, was mixed with human serum albumin (HSA). After binding to HSA, the fluorescence quantum yield increased from 5 × 10(-4) to 1.7 × 10(-2), which was enough to allow fluorescence detection and imaging of its distribution. A detection threshold, evaluated in scattering test objects, lower than 2.5 nmol × L(-1) was obtained, using a single-probe setup with a 5-mW incident light power. The detection sensitivity using a fluorescence imaging device was in the µmol × L(-1) range, with a noncooled CCD camera. Preclinical evaluation was performed on a rat model and permitted to observe inflamed nodes on all animals.
authors.affiliation: Laboratoire d'Imagerie et de Neurosciences Cognitives, UMR 7237, Université de Strasbourg/CNRS, Faculté de Médecine, 4 rue Kirschleger, 67085 Strasbourg,  France ; EURORAD, 2 rue Ettore Bugatti 67201 Eckbolsheim,  France.
authors.forename: Franklin
authors.initials: F
authors.lastname: Tellier
authors.forename: Jérôme
authors.initials: J
authors.lastname: Steibel
authors.forename: Renée
authors.initials: R
authors.lastname: Chabrier
authors.forename: François Xavier
authors.initials: FX
authors.lastname: Blé
authors.forename: Hervé
authors.initials: H
authors.lastname: Tubaldo
authors.forename: Ravelo
authors.initials: R
authors.lastname: Rasata
authors.forename: Jacques
authors.initials: J
authors.lastname: Chambron
authors.forename: Guy
authors.initials: G
authors.lastname: Duportail
authors.forename: Hervé
authors.initials: H
authors.lastname: Simon
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Rodier
authors.forename: Patrick
authors.initials: P
authors.lastname: Poulet