title: Biotransformation studies using rat proximal tubule cells.
abstract: The proximal tubule is the main target for nephrotoxic substances due to its specific properties, including efficient drug transport and biotransformation potential. The availability of a pure population of proximal tubule cells (PT cells) as a model to study a range of biological, pharmacological, and toxicological parameters is, therefore, of great value. A two-step PT cell-isolation procedure, based on density-gradient centrifugation, is described; this procedure can easily be introduced into each laboratory setting. The procedure routinely yields a highly pure PT cell population, comprising 20-40 × 10(6) cells, which can be used for preparation of subcellular fractions or brought into primary culture.
authors.affiliation: Utrecht University, Utrecht, The Netherlands.
authors.forename: G J
authors.initials: GJ
authors.lastname: Schaaf
authors.forename: R F M
authors.initials: RF
authors.lastname: Maas
authors.forename: J
authors.initials: J
authors.lastname: Fink-Gremmels
mesh_terms: D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar