title: Offspring from oocytes derived from in vitro primordial germ cell-like cells in mice.
abstract: Reconstitution of female germ cell development in vitro is a key challenge in reproductive biology and medicine. We show here that female (XX) embryonic stem cells and induced pluripotent stem cells in mice are induced into primordial germ cell-like cells (PGCLCs), which, when aggregated with female gonadal somatic cells as reconstituted ovaries, undergo X-reactivation, imprint erasure, and cyst formation, and exhibit meiotic potential. Upon transplantation under mouse ovarian bursa, PGCLCs in the reconstituted ovaries mature into germinal vesicle-stage oocytes, which then contribute to fertile offspring after in vitro maturation and fertilization. Our culture system serves as a robust foundation for the investigation of key properties of female germ cells, including the acquisition of totipotency, and for the reconstitution of whole female germ cell development in vitro.
authors.affiliation: Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan. khayashi@anat2.med.kyoto-u.ac.jp
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Hayashi
authors.forename: Sugako
authors.initials: S
authors.lastname: Ogushi
authors.forename: Kazuki
authors.initials: K
authors.lastname: Kurimoto
authors.forename: So
authors.initials: S
authors.lastname: Shimamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohta
authors.forename: Mitinori
authors.initials: M
authors.lastname: Saitou
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002868:Chromosomal Proteins, Non-Histone; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D005307:Fertilization in Vitro; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D008297:Male; D051379:Mice; D009865:Oocytes / Q000166:cytology* / Q000637:transplantation; D009866:Oogenesis; D006080:Ovarian Follicle / Q000166:cytology*; D000074462:Positive Regulatory Domain I-Binding Factor 1; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D019076:Transgenes
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C462180:Dppa3 protein, mouse; C513258:Prdm1 protein, mouse; D012097:Repressor Proteins; D014157:Transcription Factors; D000074462:Positive Regulatory Domain I-Binding Factor 1