title: 1-methylthio-D-xylulose 5-phosphate methylsulfurylase: a novel route to 1-deoxy-D-xylulose 5-phosphate in Rhodospirillum rubrum.
abstract: Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosphate (DXP), an intermediate in the nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., in press]. Dethiomethylation, a novel route to DXP, is catalyzed by MTXu 5-P methylsulfurylase. An active site Cys displaces the enolate of DXP from MTXu 5-P, generating a methyl disulfide intermediate.
authors.affiliation: Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Warlick
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Evans
authors.forename: Tobias J
authors.initials: TJ
authors.lastname: Erb
authors.forename: Udipi A
authors.initials: UA
authors.lastname: Ramagopal
authors.forename: Jaya
authors.initials: J
authors.lastname: Sriram
authors.forename: Heidi J
authors.initials: HJ
authors.lastname: Imker
authors.forename: J Michael
authors.initials: JM
authors.lastname: Sauder
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Bonanno
authors.forename: Stephen K
authors.initials: SK
authors.lastname: Burley
authors.forename: F Robert
authors.initials: FR
authors.lastname: Tabita
authors.forename: Steven C
authors.initials: SC
authors.lastname: Almo
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Sweedler
authors.forename: John A
authors.initials: JA
authors.lastname: Gerlt
mesh_terms: D053858:Metabolic Networks and Pathways; D019906:Nuclear Magnetic Resonance, Biomolecular; D010428:Pentosephosphates / Q000096:biosynthesis* / Q000378:metabolism; D012247:Rhodospirillum rubrum / Q000378:metabolism*; D012267:Ribosemonophosphates / Q000378:metabolism; D013466:Sulfurtransferases / Q000378:metabolism*; D013865:Thioglycosides / Q000378:metabolism
chemical_list: C109460:1-deoxylulose 5-phosphate; C023029:5'-methylthioribose 1-phosphate; D010428:Pentosephosphates; D012267:Ribosemonophosphates; D013865:Thioglycosides; C031625:xylulose-5-phosphate; C577158:1-methylthio-D-xylulose 5-phosphate methylsulfurylase, Rhodospirillum rubrum; D013466:Sulfurtransferases