title: A versatile multicomponent assembly via β-cyclodextrin host-guest chemistry on graphene for biomedical applications.
abstract: A multi-component nanosystem based on graphene and comprising individual cyclodextrins at its surface is assembled, creating hybrid structures enabling new and important functionalities: optical imaging, drug storage, and cell targeting for medical diagnosis and treatment. These nanohybrids are part of a universal system of interchangeable units, capable of mutilple functionalities. The surface components, made of individual β-cyclodextrin molecules, are the "hosts" for functional units, which may be used as imaging agents, for anti-cancer drug delivery, and as tumor-specific ligands. Specifically, individual β-cyclodextrin (β-CD), with a known capability to host various molecules, is considered a module unit that is assembled onto graphene nanosheet (GNS). The cyclodextrin-functionalized graphene nanosheet (GNS/β-CD) enables "host-guest" chemistry between the nanohybrid and functional "payloads". The structure, composition, and morphology of the graphene nanosheet hybrid have been investigated. The nanohybrid, GNS/β-CD, is highly dispersive in various physiological solutions, reflecting the high biostability of cyclodextrin. Regarding the host capability, the nanohybrid is fully capable of selectively accommodating various biological and functional agents in a controlled fashion, including the antivirus drug amantadine, fluorescent dye [5(6)-carboxyfluorescein], and Arg-Gly-Asp (RGD) peptide-targeting ligands assisted by an adamantine linker. The loading ratio of 5(6)-carboxyfluorescein is as high as 110% with a drug concentration of 0.45 mg mL(-1). The cyclic RGD-functionalized nanohybrid exhibits remarkable targeting for HeLa cells.
authors.affiliation: The Institute for Biomedical Engineering and Nano Science, Tongji University School of Medicine, Shanghai 200092, PR China.
authors.forename: Haiqing
authors.initials: H
authors.lastname: Dong
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Li
authors.forename: Jinhai
authors.initials: J
authors.lastname: Yu
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Song
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Cai
authors.forename: Jiaqiang
authors.initials: J
authors.lastname: Liu
authors.forename: Jiaming
authors.initials: J
authors.lastname: Zhang
authors.forename: Rodney C
authors.initials: RC
authors.lastname: Ewing
authors.forename: Donglu
authors.initials: D
authors.lastname: Shi
mesh_terms: D020811:Biomedical Technology / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; D006108:Graphite