title: Methylation dependent enhancement of trehalose production in Candida utilis.
abstract: Trehalose metabolism plays a central role in various stress responses in yeasts. Methylation dependant enhancement of trehalose synthesis has been reported from yeast Saccharomyces cerevisiae. In order to establish the role of methylation on trehalose metabolism in yeast, it was further investigated in Candida utilis. Universal methyl group donor, S-adenosyl-l-methionine (AdoMet) and its inhibitor, oxidized adenosine (Adox) were used to study the effect of methylation on trehalose metabolism in C. utilis. Treatment of early stationary phase cells of C. utilis with AdoMet and Adox exhibited increase in both intracellular metabolite levels and activities of the trehalose synthesizing enzymes, trehalose-6-phosphate synthase (TPS) and trehalose phosphate phosphatase (TPP). Among the intracellular metabolites studied, trehalose levels were enhanced in presence of AdoMet which correlated with the increasing levels of trehalose synthesizing enzymes. TPS was purified in presence of AdoMet and Adox, following an established protocol reported from this laboratory. Differences in the mobility of control TPS, methylated TPS, and methylation-inhibited TPS during acidic native gel electrophoresis confirmed the occurrence of induced methylation. MALDI-TOF analysis of trypsin-digested samples of the same further strengthened the presence of methylation in TPS. The data presented in this paper strongly indicate a positive role of methylation on trehalose synthesis which finally leads to enhanced trehalose production during the stationary growth phase of C. utilis.
authors.affiliation: Biotechnology Division, Indian Institute of Chemical Biology, 4 Raja S C Mullick Road, Kolkata 700 032, India.
authors.forename: Shinjinee
authors.initials: S
authors.lastname: Sengupta
authors.forename: Sagar
authors.initials: S
authors.lastname: Lahiri
authors.forename: Shakri
authors.initials: S
authors.lastname: Banerjee
authors.forename: Trina
authors.initials: T
authors.lastname: Dutta
authors.forename: Tarun K
authors.initials: TK
authors.lastname: Dhar
authors.forename: Anil K
authors.initials: AK
authors.lastname: Ghosh
mesh_terms: D002175:Candida / Q000166:cytology / Q000378:metabolism*; D005964:Glucosyltransferases / Q000302:isolation & purification / Q000378:metabolism; D008745:Methylation; D013997:Time Factors; D014199:Trehalose / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D014199:Trehalose; D005964:Glucosyltransferases; C023180:trehalose-6-phosphate synthase