title: Mass-producible nano-featured polystyrene surfaces for regulating the differentiation of human adipose-derived stem cells.
abstract: In this study, we report an efficient and cost-effective method of fabricating polystyrene (PS) nano-featured substrates containing nanopore (NPo) and nanopillar (NPi) arrays based on hot embossing using nickel nano-stamps. We investigate the behavior of adipose-derived stem cells (ASCs), including adhesion, morphology, proliferation and differentiation, on the replicated PS surfaces. Compared to a flat substrate, NPo- and NPi-featured substrates do not alter the morphology of stem cells. However, both NPo- and NPi-featured substrates induce different integrin expression and lower formation of focal adhesion complexes. In addition, ASCs on the NPo-featured substrate exhibit greater adipogenic differentiation, while the NPi-featured substrate induces higher osteogenic differentiation.
authors.affiliation: Department of Biomedical Science, CHA University, 502 Yatop-dong, Bundang-gu, Seongnam-si, Gyeonggi-do 463-840, Korea.
authors.forename: Kwang-Sook
authors.initials: KS
authors.lastname: Park
authors.forename: Kyoung Je
authors.initials: KJ
authors.lastname: Cha
authors.forename: In-Bo
authors.initials: IB
authors.lastname: Han
authors.forename: Dong-Ah
authors.initials: DA
authors.lastname: Shin
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Cho
authors.forename: Soo-Hong
authors.initials: SH
authors.lastname: Lee
authors.forename: Dong Sung
authors.initials: DS
authors.lastname: Kim
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects*; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D016023:Integrins / Q000235:genetics / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009532:Nickel / Q000737:chemistry; D010012:Osteogenesis / Q000187:drug effects; D011137:Polystyrenes / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D016023:Integrins; D011137:Polystyrenes; D009532:Nickel