title: Simultaneous analysis of 11 main active components in Cirsium setosum based on HPLC-ESI-MS/MS and combined with statistical methods.
abstract: A novel method based on high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry was developed for simultaneous determination of the 11 major active components including ten flavonoids and one phenolic acid in Cirsium setosum. Separation was performed on a reversed-phase C(18)  column with gradient elution of methanol and 0.1‰ acetic acid (v/v). The identification and quantification of the analytes were achieved on a hybrid quadrupole linear ion trap mass spectrometer. Multiple-reaction monitoring scanning was employed for quantification with switching electrospray ion source polarity between positive and negative modes in a single run. Full validation of the assay was carried out including linearity, precision, accuracy, stability, limits of detection and quantification. The results demonstrated that the method developed was reliable, rapid, and specific. The 25 batches of C. setosum samples from different sources were first determined using the developed method and the total contents of 11 analytes ranged from 1717.460 to 23028.258 μg/g. Among them, the content of linarin was highest, and its mean value was 7340.967 μg/g. Principal component analysis and hierarchical clustering analysis were performed to differentiate and classify the samples, which is helpful for comprehensive evaluation of the quality of C. setosum.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.forename: Lu
authors.initials: L
authors.lastname: Chang
authors.forename: Yanping
authors.initials: Y
authors.lastname: Ren
authors.forename: Liang
authors.initials: L
authors.lastname: Cao
authors.forename: Yingguang
authors.initials: Y
authors.lastname: Sun
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Du
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Shi
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
authors.forename: Lantong
authors.initials: L
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031180:Cirsium / Q000737:chemistry*; D003627:Data Interpretation, Statistical; D005419:Flavonoids / Q000737:chemistry; D015394:Molecular Structure; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts