title: Anti-metastatic and anti-angiogenic activities of sulfated polysaccharide of Sepiella maindroni ink.
abstract: A previous study demonstrated that SIP-SII, a sulfated Sepiella maindroni ink polysaccharide, suppressed the invasion and migration of cancer cells via the inhibition of the proteolytic activity of matrix metalloproteinase-2 (MMP-2). Therefore, this study investigated the anti-metastatic effect of SIP-SII in vivo. SIP-SII (15 and 30 mg/kg d) markedly decreased B16F10 pulmonary metastasis in mice models by 85.9% and 88.0%, respectively. Immunohistochemistry showed that SIP-SII decreased the expression of the intercellular adhesion molecule 1 (ICAM-1) and basic fibroblast growth factor (bFGF) in lung metastasis nodules. In addition, SIP-SII inhibited neovascularization in chick chorioallantoic membrane assay at 0.08-2 mg/mL. In the in vitro experiments, SIP-SII (0.8-500 μg/mL) significantly decreased the protein and mRNA expression of ICAM-1 and bFGF in SKOV3 and EA.hy926 cells, respectively. These results suggested that SIP-SII might suppress melanoma metastasis via the inhibition of the tumor adhesion mediated by ICAM-1 and the angiogenesis mediated by bFGF, as well as resulting in depression of the invasion and migration of carcinoma cells.
authors.affiliation: Institute of Biochemical and Biotechnological Drugs, Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, PR China.
authors.forename: Aizhen
authors.initials: A
authors.lastname: Zong
authors.forename: Ting
authors.initials: T
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xinlei
authors.initials: X
authors.lastname: Song
authors.forename: Yikang
authors.initials: Y
authors.lastname: Shi
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Cao
authors.forename: Chunhui
authors.initials: C
authors.lastname: Liu
authors.forename: Yanna
authors.initials: Y
authors.lastname: Cheng
authors.forename: Xianjun
authors.initials: X
authors.lastname: Qu
authors.forename: Jichao
authors.initials: J
authors.lastname: Cao
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D045744:Cell Line, Tumor; D049832:Decapodiformes / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism; D008546:Melanoma, Experimental / Q000098:blood supply / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009362:Neoplasm Metastasis; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D010858:Pigmentation; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013431:Sulfates / Q000737:chemistry*
chemical_list: D020533:Angiogenesis Inhibitors; D000970:Antineoplastic Agents; D011134:Polysaccharides; D012333:RNA, Messenger; D013431:Sulfates; D016222:Fibroblast Growth Factor 2; D018799:Intercellular Adhesion Molecule-1