title: Development and characterization of 11 novel microsatellite loci for the roundscale spearfish Tetrapturus georgii and their cross-species amplification among other istiophorid species.
abstract: Eleven novel polymorphic microsatellite loci were developed and characterized for the recently validated roundscale spearfish Tetrapturus georgii. Characterization of these markers, based on 35 roundscale spearfish from the western North Atlantic, revealed two to 21 alleles per locus with an average expected heterozygosity (H(E) ) of 0·09-0·94, and all loci conformed to Hardy-Weinberg expectations. Cross-amplification of these 11 loci against all other eight known istiophorid species indicates promising prospects for the utility of these markers for istiophorids in general.
authors.affiliation: Guy Harvey Research Institute, Oceanographic Center, Nova Southeastern University, Dania Beach, FL 33004, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Bernard
authors.forename: K A
authors.initials: KA
authors.lastname: Feldheim
authors.forename: M S
authors.initials: MS
authors.lastname: Shivji
mesh_terms: D000818:Animals; D017931:DNA Primers / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D010473:Perciformes / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D017931:DNA Primers