title: Protein engineering of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase.
abstract: A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7(T384Y/Q450H/V454I), produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae.
authors.affiliation: Department of Biological Science and Technology, National Chiao Tung University, 300, Hsin-Chu, Taiwan, Republic of China.
authors.forename: Yuan-Ting
authors.initials: YT
authors.lastname: Liu
authors.forename: Tain-Chang
authors.initials: TC
authors.lastname: Hu
authors.forename: Cheng-Hsiang
authors.initials: CH
authors.lastname: Chang
authors.forename: Wen-Shiang
authors.initials: WS
authors.lastname: Shie
authors.forename: Tung-Kung
authors.initials: TK
authors.lastname: Wu
mesh_terms: D003500:Cyclization; D019751:Intramolecular Transferases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D008958:Models, Molecular; D015394:Molecular Structure; D009154:Mutation; D015202:Protein Engineering; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013185:Squalene / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism
chemical_list: C520393:oxidosqualene; D013185:Squalene; D019751:Intramolecular Transferases; C021924:lanosterol synthase