title: Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata).
abstract: BACKGROUND
  Brassica oleracea encompass a family of vegetables and cabbage that are among the most widely cultivated crops. In 2009, the B. oleracea Genome Sequencing Project was launched using next generation sequencing technology. None of the available maps were detailed enough to anchor the sequence scaffolds for the Genome Sequencing Project. This report describes the development of a large number of SSR and SNP markers from the whole genome shotgun sequence data of B. oleracea, and the construction of a high-density genetic linkage map using a double haploid mapping population.
  
  
  RESULTS
  The B. oleracea high-density genetic linkage map that was constructed includes 1,227 markers in nine linkage groups spanning a total of 1197.9 cM with an average of 0.98 cM between adjacent loci. There were 602 SSR markers and 625 SNP markers on the map. The chromosome with the highest number of markers (186) was C03, and the chromosome with smallest number of markers (99) was C09.
  
  
  CONCLUSIONS
  This first high-density map allowed the assembled scaffolds to be anchored to pseudochromosomes. The map also provides useful information for positional cloning, molecular breeding, and integration of information of genes and traits in B. oleracea. All the markers on the map will be transferable and could be used for the construction of other genetic maps.
authors.affiliation: Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, PR China, Beijing, 100081, China.
authors.forename: Wanxing
authors.initials: W
authors.lastname: Wang
authors.forename: Shunmou
authors.initials: S
authors.lastname: Huang
authors.forename: Yumei
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fang
authors.forename: Limei
authors.initials: L
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
authors.forename: Suxia
authors.initials: S
authors.lastname: Yuan
authors.forename: Shengyi
authors.initials: S
authors.lastname: Liu
authors.forename: Jifeng
authors.initials: J
authors.lastname: Sun
authors.forename: Mu
authors.initials: M
authors.lastname: Zhuang
authors.forename: Yangyong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Aisong
authors.initials: A
authors.lastname: Zeng
mesh_terms: D001937:Brassica / Q000235:genetics*; D002874:Chromosome Mapping; D005819:Genetic Markers / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D005819:Genetic Markers