title: Effect of Lecithin on d-Galactosamine Induced Hepatotoxicity Through Mitochondrial Pathway Involving Bcl-2 and Bax.
abstract: Twenty four Wistar strain albino rats were used for the investigations. Lecithin 50 and 100 mg/kg b wt was administered for 1 week by oral route. Liver damage was induced by intra peritoneal administration of 400 mg/kg b wt d-galactosamine on the last day. At the end of the study animals were sacrificed and liver enzyme levels, histopathology, mitochondrial integrity, expression of p53, Bax and Bcl-2 mRNA levels were studied. Increases in the liver enzyme levels by d-GalN were significantly inhibited by pretreatment with lecithin. Histopathological observation further confirmed the hepatoprotective effect of lecithin. In addition, the disruption of mitochondrial membrane, up regulation of Bax and down regulation of Bcl-2 mRNA levels in the liver of d-GalN intoxicated rats were effectively prevented by pretreatment with lecithin. The results of the present study validate our conviction that d-GalN causes hepatic damage via mitochondrial pathway involving Bax and Bcl-2.
authors.affiliation: Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, 576104 Karnataka India.
authors.forename: P Vasanth
authors.initials: PV
authors.lastname: Raj
authors.forename: K
authors.initials: K
authors.lastname: Nitesh
authors.forename: Jain
authors.initials: J
authors.lastname: Prateek
authors.forename: M Neena
authors.initials: MN
authors.lastname: Sankhe
authors.forename: J Venkata
authors.initials: JV
authors.lastname: Rao
authors.forename: C Mallikarjuna
authors.initials: CM
authors.lastname: Rao
authors.forename: N
authors.initials: N
authors.lastname: Udupa