title: Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration.
abstract: Surface modification of biomaterials has been shown to improve the biological response to dental implants. The ability to create a controlled micro-texture on the implant via additive surface modification techniques with bioactive nanohydroxyapatite (nanoHA) may positively influence guided tissue regeneration.
  
  
  OBJECTIVE
  The main goal of this study was to produce micro-fabricated SiO(2) surfaces modified with nanohydroxyapatite particles and to characterize their influence on the biological response of Human Dental-Pulp Mesenchymal Stem Cells (hDP-MSCs) and Streptococcus mutans.
  
  
  METHODS
  A combined methodology of sol-gel and soft-lithography was used to produce micropatterned SiO(2) thin films with different percentages of nanoHA micro-aggregates. The surfaces were characterized by SEM/EDS, FT-IR/ATR, AFM, XPS quantitative elemental percentage and contact angle measurements. Biological characterization was performed using hDP-MSCs cultures, while Streptococcus mutans was the selected microorganism to evaluate the bacterial adhesion on the thin films.
  
  
  RESULTS
  Micropatterned SiO(2) surfaces with 0%, 1% and 5% of nanoHA micro-aggregates were successfully produced using a combination of sol-gel and soft-lithography. These surfaces controlled the biological response, triggering alignment and oriented proliferation of hDP-MSCs and significant differences in the adhesion of S. mutans to the different surfaces.
  
  
  CONCLUSIONS
  The micropatterned surfaces exhibited biocompatible behavior that induced an oriented adhesion and proliferation of hDP-MSCs while SiO(2) presented low bacterial adhesion. These results show that the combination of sol-gel with soft-lithography is a good approach to create micropatterned surfaces with bioactive nanoparticles for guided tissue regeneration.
authors.affiliation: INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Rua Campo Alegre, 823, 4150-180 Porto, Portugal. angela.carvalho@ineb.up.pt
authors.forename: A
authors.initials: A
authors.lastname: Carvalho
authors.forename: A
authors.initials: A
authors.lastname: Pelaez-Vargas
authors.forename: D
authors.initials: D
authors.lastname: Gallego-Perez
authors.forename: L
authors.initials: L
authors.lastname: Grenho
authors.forename: M H
authors.initials: MH
authors.lastname: Fernandes
authors.forename: A H
authors.initials: AH
authors.lastname: De Aza
authors.forename: M P
authors.initials: MP
authors.lastname: Ferraz
authors.forename: D J
authors.initials: DJ
authors.lastname: Hansford
authors.forename: F J
authors.initials: FJ
authors.lastname: Monteiro
mesh_terms: D016880:Anisotropy; D001422:Bacterial Adhesion / Q000502:physiology; D001672:Biocompatible Materials / Q000737:chemistry*; D018441:Biofilms; D002448:Cell Adhesion / Q000502:physiology; D002452:Cell Count; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017076:Computer-Aided Design; D003764:Dental Materials / Q000737:chemistry*; D003782:Dental Pulp / Q000166:cytology; D017886:Durapatite / Q000737:chemistry*; D048091:Guided Tissue Regeneration / Q000379:methods; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000502:physiology; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry*; D044367:Phase Transition; D056951:Photoelectron Spectroscopy; D012822:Silicon Dioxide / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013295:Streptococcus mutans / Q000502:physiology; D013499:Surface Properties; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D003764:Dental Materials; D012822:Silicon Dioxide; D017886:Durapatite