title: ADSA Foundation Scholar Award: Trends in culture-independent methods for assessing dairy food quality and safety: emerging metagenomic tools.
abstract: Enhancing the quality and safety of dairy food is critical to maintaining the competitiveness of dairy products in the food and beverage market and in reinforcing consumer confidence in the dairy industry. Raw milk quality has a significant effect on finished product quality. Several microbial groups found in raw milk have been shown to adversely affect the shelf life of pasteurized milk. Current microbiological criteria used to define milk quality are based primarily on culture-dependent methods, some of which are perceived to lack the desired sensitivity and specificity. To supplement traditional methods, culture-independent methods are increasingly being used to identify specific species or microbial groups, and to detect indicator genes or proteins in raw milk or dairy products. Some molecular subtyping techniques have been developed to track the transmission of microbes in dairy environments. The burgeoning "-omics" technologies offer new and exciting opportunities to enhance our understanding of food quality and safety in relation to microbes. Metagenomics has the potential to characterize microbial diversity, detect nonculturable microbes, and identify unique sequences or other factors associated with dairy product quality and safety. In this review, fluid milk will be used as the primary example to examine the adequacy and validity of conventional methods, the current trend of culture-independent methods, and the potential applications of metagenomics in dairy food research.
authors.affiliation: California Polytechnic State University, San Luis Obispo, CA 93407, USA. pmyeung@calpoly.edu
authors.forename: Marie
authors.initials: M
authors.lastname: Yeung
mesh_terms: D000818:Animals; D002417:Cattle; D016172:DNA Fingerprinting; D003611:Dairy Products / Q000592:standards*; D005516:Food Microbiology / Q000379:methods / Q000592:standards; D005519:Food Preservation / Q000379:methods; D059022:Food Safety / Q000379:methods*; D056186:Metagenomics / Q000379:methods*; D008892:Milk / Q000382:microbiology / Q000592:standards; D061352:Pasteurization / Q000379:methods