title: Partial Purification and Characterization of an Elastase-inhibitory Lipid Derivative from a Cyanobacterium, Microcystis Ku2.
abstract: A unicyanobacterial isolate of cyanobacterium, identified as Microcystis Ku2, produced a mammalian elastase-inhibitory lipid derivative. Protease inhibitors in cyanobacteria are unequivocally peptides. Since this metabolite appeared in lipid phase, we worked on a hypothesis that whether metabolite other than peptides could be responsible for the characteristic inhibition. It was purified by saponification and reverse phase column chromatography. The resulting compound was tentatively characterized as a glycolipid with structure of sulfated di-pentose derivative of ceramide (MW = 956 Da). The apparent IC(50) for elastase was 1.3 μM.
authors.affiliation: Department of Biological Sciences, Rani Durgavati University, Jabalpur, 482001 India.
authors.forename: Suvendra Nath
authors.initials: SN
authors.lastname: Bagchi
authors.forename: Shubhro Kamal
authors.initials: SK
authors.lastname: Ghosh