title: Production and immunogenicity of chimeric virus-like particles containing porcine reproductive and respiratory syndrome virus GP5 protein.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) poses a severe threat in swine industry and causes heavy economic losses worldwide. Currently, the available vaccines are the inactivated and attenuated virus vaccines, but the use of PRRSV in their production raises the issue of safety. We developed a chimeric virus-like particles (VLPs) vaccine candidate for PRRSV protection. The chimeric VLPs was composed of M1 protein from H1N1 influenza virus and a fusion protein, denoted as NA/GP5, containing the cytoplasmic and transmembrane domains of H1N1 virus NA protein and PRRSV GP5 protein. Vaccination of BALB/c mice with 10 μg of chimeirc VLPs by intramuscular immunization stimulated antibody responses to GP5 protein, and induced cellular immune response. The data suggested that the chimeric VLP vaccine candidate may provide a new strategy for further development of vaccines against PRRSV infection.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Chen
authors.forename: Chunyi
authors.initials: C
authors.lastname: Xue
authors.forename: Yichao
authors.initials: Y
authors.lastname: Du
authors.forename: Lishan
authors.initials: L
authors.lastname: Lv
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Liu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Ma
authors.forename: Huifang
authors.initials: H
authors.lastname: Shen
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D007273:Injections, Intramuscular; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000235:genetics; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; C488936:M1 protein, Influenza A virus; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D058425:Vaccines, Virus-Like Particle; D014759:Viral Envelope Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins; C114789:glycoprotein 5, PRRSV; C487630:NA protein, influenza A virus; D009439:Neuraminidase