title: Arcanobacterium phocisimile sp. nov., isolated from harbour seals.
abstract: A polyphasic taxonomic study was performed on two previously unidentified Arcanobacterium-like Gram-positive strains isolated from harbour seals. Comparative 16S rRNA gene sequencing showed that both bacteria belonged to the genus Arcanobacterium and were most closely related to Arcanobacterium haemolyticum CIP 103370(T) (98.4% 16S rRNA gene sequence similarity), A. canis P6775(T) (97.4%), A. phocae DSM 10002(T) (97.4%), A. pluranimalium M430/94/2(T) (95.7%) and A. hippocoleae CCUG 44697(T) (95.5%). The presence of the major menaquinone MK-9(H4) supported the affiliation of the isolates with the genus Arcanobacterium. The polar lipid profile consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and two unidentified glycolipids. The major fatty acids were C16:0, C18:0, C18:1ω9c and summed feature 5 (comprising C18:2ω6,9c and/or anteiso-C18:0). Physiological and biochemical tests clearly distinguished the isolates from other members of the genus Arcanobacterium. Based on the common origin and various physiological properties comparable to those of A. phocae, it is proposed that the isolates are classified as members of a novel species with the name Arcanobacterium phocisimile sp. nov. The type strain is 2698(T) (=LMG 27073(T) =CCM 8430(T)).
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.
authors.forename: Muaz
authors.initials: M
authors.lastname: Hijazin
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.
authors.forename: Osama
authors.initials: O
authors.lastname: Sammra
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.
authors.forename: Hivda
authors.initials: H
authors.lastname: Ülbegi-Mohyla
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.
authors.forename: Samy
authors.initials: S
authors.lastname: Nagib
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Alber
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Lämmler
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Kassmannhuber
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.
authors.forename: Ellen
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Giessen, 35392 Giessen, Germany.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Weiss
authors.affiliation: Forschungs- und Technologiezentrum Westküste, Zentrale Einrichtung der Christian-Albrechts-Universität zu Kiel, 25761 Büsum, Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Siebert
authors.affiliation: Department of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
authors.forename: Abdulwahed Ahmed
authors.initials: AA
authors.lastname: Hassan
authors.affiliation: Institut für Lebensmittelqualität und -sicherheit, Stiftung Tierärztliche Hochschule Hannover, 30173 Hannover, Germany.
authors.forename: Amir
authors.initials: A
authors.lastname: Abdulmawjood
authors.affiliation: Landesbetrieb Hessisches Landeslabor, 35392 Giessen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Zschöck
mesh_terms: D000818:Animals; D055667:Arcanobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D046049:Phoca / Q000382:microbiology*; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S