title: Cytokine secretion from human monocytes potentiated by P-selectin-mediated cell adhesion.
abstract: OBJECTIVE
  P-selectin is a carbohydrate-recognizing cell adhesion molecule expressed on activated platelets and endothelial cells. It plays a crucial role in the recruitment of leukocytes to inflammatory and hemorrhagic sites. Cell adhesion mediated by P-selectin induces leukocyte activation, such as the generation of reactive oxygen species and the expression of blood coagulation factors. We assessed how P-selectin-mediated cell adhesion affects cytokine secretion from monocytes.
  
  
  METHODS
  Human peripheral blood monocytes were cultured in a plate that had been coated with P-selectin purified from human platelets, and cytokines released in the culture supernatant from monocytes were determined by ELISA.
  
  
  RESULTS
  The secretion of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, IL-12 and macrophage inflammatory protein-1β increased 3- to 10-fold in response to P-selectin compared with unstimulated monocytes. We next examined the effects of cytokine treatment of monocytes on their susceptibility to P-selectin. The secretion of TNF-α from monocytes in response to P-selectin was increased when monocytes were preincubated with granulocyte/macrophage colony-stimulating factor, monocyte chemotactic protein-1 or interferon-γ (IFN-γ); IFN-γ was the most effective in potentiating TNF-α secretion from monocytes.
  
  
  CONCLUSIONS
  These results suggest that the interaction of monocytes with P-selectin plays an important role not only in their trafficking but also in the regulation of cytokine production by these cells.
authors.affiliation: Department of Microbiology, Hoshi University School of Pharmacy and Pharmaceutical Sciences, University of Tokyo, Tokyo, Japan.
authors.forename: Junsuke
authors.initials: J
authors.lastname: Suzuki
authors.forename: Eri
authors.initials: E
authors.lastname: Hamada
authors.forename: Tomonori
authors.initials: T
authors.lastname: Shodai
authors.forename: Go
authors.initials: G
authors.lastname: Kamoshida
authors.forename: Sanae
authors.initials: S
authors.lastname: Kudo
authors.forename: Saotomo
authors.initials: S
authors.lastname: Itoh
authors.forename: Junzo
authors.initials: J
authors.lastname: Koike
authors.forename: Kisaburo
authors.initials: K
authors.lastname: Nagata
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Irimura
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D002448:Cell Adhesion; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D019007:P-Selectin / Q000378:metabolism*
chemical_list: D016207:Cytokines; D019007:P-Selectin