title: TNF-alpha rs1800629 polymorphism is not associated with HPV infection or cervical cancer in the Chinese population.
abstract: BACKGROUND
  While HPV infection is the main cause of cervical cancer, genetic susceptibility to HPV infection is not well understood. Tumor necrosis factor alpha (TNF-alpha), involved in the defense against HPV infection, plays an important role in cervical cancer progression and regression. The aim of this study was to investigate the relationship between the TNF-alpha rs1800629 polymorphism and risk of HPV infection or cervical cancer.
  
  
  METHODS
  Three groups were involved in this study of Chinese women. Group 1 consisted of 285 high risk HPV positive cervical cancer patients, Group 2, 225 high risk HPV positive patients without cervical cancer, and Group 3, 318 HPV negative women with no cervical cancer. Blood samples were obtained from all patients and genotyped by PCR-RLFP. Fifty randomly selected samples were further sequenced.
  
  
  RESULTS
  The allele and genotype distributions of the TNF-alpha rs1800629 polymorphism were not significantly different between each of the groups (P>0.05). There are no significant relationship between rs1800629 polymorphism and high risk HPV infection (OR = 0.649, 95% CI: 0.253-1.670, P = 0.371), cervical cancer (OR = 0.993, 95% CI: 0.376-2.618, P = 0.988), or cervical cancer with HPV infection (OR = 0.663, 95% CI: 0.250-1.758, P = 0.409).
  
  
  CONCLUSIONS
  We demonstrated that there is no association between TNF rs1800629 polymorphism and the HPV infection, or cervical cancer with HPV infection.
authors.affiliation: Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Duo
authors.initials: D
authors.lastname: Yin
authors.forename: Shulan
authors.initials: S
authors.lastname: Zhang
authors.forename: Heng
authors.initials: H
authors.lastname: Wei
authors.forename: Shizhuo
authors.initials: S
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yanming
authors.initials: Y
authors.lastname: Lu
authors.forename: Shuyan
authors.initials: S
authors.lastname: Dai
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000483:Alleles; D002681:China / Q000453:epidemiology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000502:physiology*; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000821:virology
chemical_list: D014409:Tumor Necrosis Factor-alpha