title: Isolation of yellow fever virus from mosquitoes in Misiones province, Argentina.
abstract: Yellow fever (YF) is a viral hemorrhagic fever endemic to tropical regions of South America and Africa. From 2007 to 2009 an important epidemic/epizootic of YF was detected in different populations of howler monkeys (Alouatta species) in Misiones, a northeastern Argentinian province. Yellow fever virus (YFV) infection was researched and documented by laboratory tests in humans and in dead Alouatta carayá. The objective of that research was to investigate the circulation of YFV in mosquitoes, which could be implicated in the sylvatic transmission of YF in Argentina. The above-mentioned mosquitoes were captured in the same geographical region where the epizootic took place. A YFV strain was isolated in cell culture from pools of Sabethes albiprivus. This study is not only the first isolation of YFV from mosquitoes in Argentina, but it is also the first YFV isolation reported in the species Sabethes albiprivus, suggesting that this species might be playing a key role in sylvatic YF in Argentina.
authors.affiliation: Instituto Nacional de Enfermedades Virales Humanas, Pergamino, Buenos Aires, Argentina. silvinagoenaga@hotmail.com
authors.forename: Silvina
authors.initials: S
authors.lastname: Goenaga
authors.forename: Cintia
authors.initials: C
authors.lastname: Fabbri
authors.forename: Juan Climaco Rondan
authors.initials: JC
authors.lastname: Dueñas
authors.forename: Cristina Noemí
authors.initials: CN
authors.lastname: Gardenal
authors.forename: Gustavo Carlos
authors.initials: GC
authors.lastname: Rossi
authors.forename: Gladys
authors.initials: G
authors.lastname: Calderon
authors.forename: Maria Alejandra
authors.initials: MA
authors.lastname: Morales
authors.forename: Jorge Braulio
authors.initials: JB
authors.lastname: Garcia
authors.forename: Delia Alcira
authors.initials: DA
authors.lastname: Enria
authors.forename: Silvana
authors.initials: S
authors.lastname: Levis
mesh_terms: D000508:Alouatta; D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001483:Base Sequence; D001499:Bayes Theorem; D018929:Cell Culture Techniques; D009033:Culicidae / Q000821:virology*; D004196:Disease Outbreaks; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D008969:Molecular Sequence Data; D008992:Monkey Diseases / Q000453:epidemiology / Q000821:virology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D015004:Yellow Fever / Q000453:epidemiology / Q000635:transmission* / Q000821:virology; D015005:Yellow fever virus / Q000302:isolation & purification*; D015047:Zoonoses