title: Spatial structure of heptapeptide Aβ(16-22)  (beta-amyloid Aβ(1-40)  active fragment) in solution and in complex with a biological membrane model.
abstract: The spatial structure of an active fragment of beta-amyloid Aβ(1-40) heptapeptide Aβ(16-22) (Lys-Leu-Val-Phe-Phe-Ala-Glu) in aqueous buffer solution and in complex with sodium dodecyl sulfate micelles as a model membrane system was investigated by (1)H NMR spectroscopy and two-dimensional NMR (TOCSY, HSQC-HECADE (Heteronuclear Couplings from ASSCI-domain experiments with E.COSY-type crosspeaks), NOESY) spectroscopy. Complex formation was confirmed by the chemical shift changes of the heptapeptide's (1)H NMR spectra, as well as by the signs and values of the NOE effects in different environments. We compared the spatial structure of the heptapeptide in borate buffer solution and in complex with a model of the cell surface membrane.
authors.affiliation: Kazan (Volga Region) Federal University, 18 Kremlevskaya St., 420008, Kazan, Russian Federation.
authors.forename: Konstantin S
authors.initials: KS
authors.lastname: Usachev
authors.forename: Sergej V
authors.initials: SV
authors.lastname: Efimov
authors.forename: Ajdar R
authors.initials: AR
authors.lastname: Yulmetov
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Filippov
authors.forename: Oleg N
authors.initials: ON
authors.lastname: Antzutkin
authors.forename: Sergii
authors.initials: S
authors.lastname: Afonin
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Klochkov
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D001888:Boric Acids; D002021:Buffers; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D008823:Micelles; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011522:Protons; D012967:Sodium Dodecyl Sulfate; D012996:Solutions; D014867:Water
chemical_list: D016229:Amyloid beta-Peptides; D001888:Boric Acids; D002021:Buffers; D008567:Membranes, Artificial; D008823:Micelles; D010446:Peptide Fragments; D011522:Protons; D012996:Solutions; D014867:Water; D012967:Sodium Dodecyl Sulfate; C032688:boric acid