title: Azoreductase in Staphylococcus aureus.
abstract: Azoreductase(s) catalyze a NAD(P)H-dependent reaction in bacteria to metabolize azo dyes to colorless aromatic amines. Azoreductases from bacteria represent a novel family of enzymes with little similarity to other reductases. This unit will describe the current methods for measuring azoreductase from Staphylococcus aureus, which has been suggested to serve as a model strain to study the azo dye degradation by human skin microflora.
authors.affiliation: National Center for Toxicological Research/FDA, Jefferson, Arkansas, USA.
authors.forename: Wen
authors.initials: W
authors.lastname: Zou
authors.forename: Carl E
authors.initials: CE
authors.lastname: Cerniglia
authors.forename: Huizhong
authors.initials: H
authors.lastname: Chen
mesh_terms: D001391:Azo Compounds / Q000378:metabolism / Q000493:pharmacokinetics; D001711:Biotransformation; D004396:Coloring Agents / Q000378:metabolism / Q000493:pharmacokinetics; D006801:Humans; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009601:Nitroreductases; D012867:Skin / Q000382:microbiology; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D009247:NADH, NADPH Oxidoreductases; D009601:Nitroreductases; C021106:azoreductase