title: Coaxially electrospun scaffolds based on hydroxyl-functionalized poly(ε-caprolactone) and loaded with VEGF for tissue engineering applications.
abstract: The aim of this study was to fabricate nanofibrous scaffolds based on blends of a hydroxyl functionalized polyester (poly(hydroxymethylglycolide-co-ε-caprolactone), pHMGCL) and poly(ε-caprolactone) (PCL), loaded with bovine serum albumin (BSA) as a protein stabilizer and vascular endothelial growth factor (VEGF) as a potent angiogenic factor by means of a coaxial electrospinning technique. The scaffolds were characterized by scanning electron microscopy (SEM), fluorescence microscopy (FM), and differential scanning calorimetry (DSC). The scaffolds displayed a uniform fibrous structure with a fiber diameter around 700 nm. The release of BSA from the core of the fibers was studied by high performance liquid chromatography (HPLC), and it was shown that the coaxial scaffolds composed of blends of pHMGCL and PCL exhibited faster release than the comparative PCL scaffolds. VEGF was also incorporated in the core of the scaffolds, and the effect of the released protein on the attachment and proliferation of endothelial cells was investigated. It was shown that the incorporated protein preserved its biological activity and resulted in initial higher numbers of adhered cells. Thus, these bioactive scaffolds based on blends of pHMGCL/PCL loaded with VEGF can be considered as a promising candidate for tissue engineering applications.
authors.affiliation: Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Faculty of Science, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands.
authors.forename: Hajar
authors.initials: H
authors.lastname: Seyednejad
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.forename: Cornelus F
authors.initials: CF
authors.lastname: van Nostrum
authors.forename: Tina
authors.initials: T
authors.lastname: Vermonden
authors.forename: Jeroen J J P
authors.initials: JJ
authors.lastname: van den Beucken
authors.forename: Wouter J A
authors.initials: WJ
authors.lastname: Dhert
authors.forename: Wim E
authors.initials: WE
authors.lastname: Hennink
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
mesh_terms: D002460:Cell Line; D006801:Humans; D011091:Polyesters / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D011091:Polyesters; D042461:Vascular Endothelial Growth Factor A; C016240:polycaprolactone; D012710:Serum Albumin, Bovine