title: Application of SSH and quantitative real time PCR to construction of gene expression profiles from scallop Chlamys farreri in response to exposure to tetrabromobisphenol A.
abstract: TBBPA-induced genes were identified using suppression subtractive hybridization (SSH) from Chlamys farreri. A total of 203 and 44 clones from SSH forward and reverse library were respectively obtained including cellular process, immune system process, response to stimulus, metabolic process and signaling etc. Differential gene expressions were compared between scallops from control and TBBPA treatment groups (400 μg/L, 15 days) using quantitative real time RT-PCR. For further research, eight significant genes expression from scallops exposed to TBBPA (0; 100; 200; 400 μg/L) sampling at 0, 1, 3, 6 and 15 days, were utilized for Q-RT-PCR. The results revealed that the expression level of most selected cDNAs was dominantly up-regulated or down-regulated in the TBBPA-induced scallops. These findings provide basic genomic information of the bivalve and the selected genes may be the potential molecular biomarkers for TBBPA pollution in aquatic environment.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Gong
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
authors.forename: Jingjing
authors.initials: J
authors.lastname: Miao
authors.forename: Na
authors.initials: N
authors.lastname: Liu
mesh_terms: D000818:Animals; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D049897:Pectinidae / Q000235:genetics; D011075:Polybrominated Biphenyls / Q000633:toxicity*; D060888:Real-Time Polymerase Chain Reaction; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D011075:Polybrominated Biphenyls; D014874:Water Pollutants, Chemical; C020806:tetrabromobisphenol A