title: Potential of Piper guineense and Aframomum longiscapum to reduce radiation induced hepatic damage in male Wistar rats.
abstract: The ameliorative effect of aqueous extracts of Piper guineense and Aframomum longiscapum on radiation-induced hepatic damage was evaluated. Rats were treated with a single dose of 600 rads (6 Gy) y-radiation to induce hepatic damage. Aqueous extracts of Piper guineense and Aframomum longiscapum (200 and 400 mg/kg b. wt) were administered orally to rats for two weeks prior to radiation and four weeks after radiation. Hepatic malondialdehyde (MDA), glutathione (GSH) levels and glutathione peroxidase (GPx) and catalase (CAT) activities were determined for their antioxidant capacity. The activities of serum markers such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and the histological changes were examined to evaluate potential ameliorative effects. Results from this study confirmed that exposure of animals to radiation led to induction of lipid peroxidation (LPO), reduced the level of GSH as well as CAT and GPx activities while simultaneously a significant elevation in the activities of serum ALT and AST was observed. Administration of varying doses of P.G. and A.L. before and after irradiation inhibited the elevated levels of LPO, restored the GSH level and enhanced CAT and GPx activities as well as significantly decreased the elevated levels of serum ALT and AST activities. This findings demonstrated that aqueous extracts of Piper guineense and Aframomum longiscapum might mitigate the liver gamma-radiation-induced damage probably by increasing antioxidant activities.
authors.affiliation: Nutritional Research Laboratory, Department of Biochemistry, Faculty of Basic Medical Science, College of Medicine, University of Ibadan, Ibadan, Nigeria sonwozo@yahoo.com
authors.forename: S O
authors.initials: SO
authors.lastname: Nwozo
authors.forename: P E
authors.initials: PE
authors.lastname: Okameme
authors.forename: B E
authors.initials: BE
authors.lastname: Oyinloye
mesh_terms: D000818:Animals; D002374:Catalase / Q000032:analysis; D005720:Gamma Rays; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000187:drug effects / Q000293:injuries / Q000528:radiation effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D031701:Piper; D010936:Plant Extracts / Q000008:administration & dosage*; D011833:Radiation Injuries, Experimental; D011837:Radiation-Protective Agents / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000032:analysis; D030521:Zingiberaceae
chemical_list: D010936:Plant Extracts; D011837:Radiation-Protective Agents; D002374:Catalase; D013482:Superoxide Dismutase