title: Retroviral integrase proteins and HIV-1 DNA integration.
abstract: Retroviral integrases catalyze two reactions, 3'-processing of viral DNA ends, followed by integration of the processed ends into chromosomal DNA. X-ray crystal structures of integrase-DNA complexes from prototype foamy virus, a member of the Spumavirus genus of Retroviridae, have revealed the structural basis of integration and how clinically relevant integrase strand transfer inhibitors work. Underscoring the translational potential of targeting virus-host interactions, small molecules that bind at the host factor lens epithelium-derived growth factor/p75-binding site on HIV-1 integrase promote dimerization and inhibit integrase-viral DNA assembly and catalysis. Here, we review recent advances in our knowledge of HIV-1 DNA integration, as well as future research directions.
authors.affiliation: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, Massachusetts 02215, USA.
authors.forename: Lavanya
authors.initials: L
authors.lastname: Krishnan
authors.forename: Alan
authors.initials: A
authors.lastname: Engelman
mesh_terms: D002384:Catalysis; D020134:Catalytic Domain; D018360:Crystallography, X-Ray / Q000379:methods; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D019281:Dimerization; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D019426:Integrases / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D014176:Protein Biosynthesis; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D016092:Spumavirus / Q000235:genetics; D016662:Virus Integration
chemical_list: D004279:DNA, Viral; D036341:Intercellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; C119380:lens epithelium-derived growth factor; D004247:DNA; D019426:Integrases