title: No single way to explain cytoplasmic maturation of oocytes from prepubertal and cyclic gilts.
abstract: The objective of this study was to evaluate selected aspects of cytoplasmic maturation in oocytes from prepubertal and cyclic crossbred gilts before and after in vitro maturation. For this purpose, cortical granule redistribution, mitochondrial DNA content and mitochondria translocation were analyzed. Moreover, for the first time the fatty acid profiles in follicular fluid (FF) of both gilt categories was evaluated. The nuclear maturation (the percentage of metaphase II oocytes was 83% in prepubertal gilts compared with 87% in cyclic gilts), cortical granule relocation from the cortex to peripheral ooplasm (98.7% vs. 98.8% of oocytes, respectively) and mitochondrial DNA content (227 543 vs. 206 660, respectively) was not affected by sexual maturity of the donor gilt. However, the redistribution of active mitochondria during in vitro maturation was observed only in the oocytes of cyclic gilts. With regard to FF analysis, saturated, unsaturated, and monounsaturated fatty acids were significantly more abundant in the FF of prepubertal females. In particular, stearic (C18:0) and palmitic (C16:0) fatty acids had significantly higher concentrations in the FF of prepubertal gilts. In conclusion, although the oocytes of prepubertal gilts matured in vitro at a rate similar to those of cyclic gilts, they differed with respect to the selected factors attributed to cytoplasmic maturation. We suggest that the higher content of particular fatty acids, which is known to have a negative influence on oocyte maturation, as well as impaired mitochondria redistribution are factors limiting the maturation potential of oocytes from prepubertal gilts.
authors.affiliation: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, Poznan, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Pawlak
authors.forename: A
authors.initials: A
authors.lastname: Cieslak
authors.forename: E
authors.initials: E
authors.lastname: Warzych
authors.forename: Z
authors.initials: Z
authors.lastname: Zejden
authors.forename: M
authors.initials: M
authors.lastname: Szumacher-Strabel
authors.forename: M
authors.initials: M
authors.lastname: Molinska-Glura
authors.forename: D
authors.initials: D
authors.lastname: Lechniak
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000502:physiology; D004272:DNA, Mitochondrial; D030762:Estrous Cycle / Q000502:physiology*; D005227:Fatty Acids; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D009865:Oocytes / Q000502:physiology*; D012741:Sexual Maturation / Q000502:physiology*; D013552:Swine / Q000254:growth & development* / Q000502:physiology
chemical_list: D004272:DNA, Mitochondrial; D005227:Fatty Acids