title: [Isolation and functional characterization of lipase from the thermophilic alkali-tolerant bacterium Thermosyntropha lipolytica].
abstract: As a result of sequencing the genome of the termophilic alkali-tolerant lipolytic bacterium Thermosyntropha lipolytica, the gene encoding a lipase secreted into the medium was identified. The recombinant enzyme was expressed in Escherichia coli. It was isolated, purified, and functionally characterized. The lipase exhibited hydrolytic activity toward para-nitrophenyl esters of various chain lengths, as well as triglycerides, including vegetable oils. The optimal reaction conditions were achieved at temperatures from 70 to 80 degrees C and pH 8.0. Enzyme saved more than 80% of its activity in the presence of 10% methanol. This new thermostable lipase may be a promising biocatalyst for organic synthesis; it may find application in the food and detergent industry and biodiesel production.
authors.forename: V M
authors.initials: VM
authors.lastname: Gumerov
authors.forename: A V
authors.initials: AV
authors.lastname: Mardanov
authors.forename: P M
authors.initials: PM
authors.lastname: Kolosov
authors.forename: N V
authors.initials: NV
authors.lastname: Ravin
mesh_terms: D000468:Alkalies; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli; D016680:Genome, Bacterial; D016936:Gram-Positive Endospore-Forming Bacteria / Q000201:enzymology* / Q000235:genetics; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008066:Lipolysis; D008969:Molecular Sequence Data; D009596:Nitrophenols; D010938:Plant Oils / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000468:Alkalies; D001426:Bacterial Proteins; D009596:Nitrophenols; D010938:Plant Oils; D011994:Recombinant Proteins; D014280:Triglycerides; C024719:4-nitrophenyl; D008049:Lipase