title: [Expressions of NF-κBp65 and IκBα in gestational trophoblastic disease and clinical significance].
abstract: OBJECTIVE
  To investigate the roles of nuclear factor κB p65 (NF-κBp65) and inhibitor of nuclear factor κB α (IκBα) in the development and metastasis of gestational trophoblastic neoplasia (GTN) by analyzing the expressions of NF-κBp65 and IκBα in normal early pregnancy villi and gestational trophoblastic diseases,  and to reveal the relationship of NF-κBp65 and IκBα with age and clinical stage of GTN patients.
  
  
  METHODS
  The expressions of NF-κBp65 and IκBα were detected by immunohistochemistry in normal pregnancy villi (20 cases),  hydatidiform moles (HM,  30 cases),  invasive hydatidiform moles (IHM,  13 cases) and chorionic carcinomas (CCA,  15 cases).
  
  
  RESULTS
  NF-κBp65 expression was statistically different (P<0.05) between normal pregnancy villi and IHM (P=0.013),  normal pregnancy villi and CCA(P=0.018),  HM and IHM(P=0.026),  HM and CCA (P=0.035). Differences in IκBα expression were statistically significant between normal pregnancy villi and IHM,  normal pregnancy villi and CCA,  HM and IHM,  HM and CCA (P<0.01). The expressions of NF-κBp65 and IκBα were correlated to clinical stage (P=0.043,  0.042,  P<0.05),  but not to patients' ages. Spearman correlation analysis revealed that there was a negative association between the protein expressions of NF-κBp65 and IκBα in GTN (r=-0.403,  P=0.034,  P<0.05).
  
  
  CONCLUSIONS
  Up-regulated expression of NF-κBp65 and down-regulated expression of IκBα may be related to the development,  invasion and metastasis of GTN. The expressions of NF-κBp65 and IκBα are negatively correlated in gestational trophoblastic tumor tissues.
authors.affiliation: Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha, China.
authors.forename: Ling-li
authors.initials: LL
authors.lastname: Ju
authors.forename: Hui-ning
authors.initials: HN
authors.lastname: Liu
authors.forename: Jing-ting
authors.initials: JT
authors.lastname: Cai
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
mesh_terms: D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D015972:Gene Expression Regulation, Neoplastic; D031901:Gestational Trophoblastic Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020672:I-kappa B Proteins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008875:Middle Aged; D000072000:NF-KappaB Inhibitor alpha; D009367:Neoplasm Staging; D011247:Pregnancy; D051996:Transcription Factor RelA / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D020672:I-kappa B Proteins; C000606204:NFKBIA protein, human; D051996:Transcription Factor RelA; D000072000:NF-KappaB Inhibitor alpha