title: GPR54-dependent stimulation of luteinizing hormone secretion by neurokinin B in prepubertal rats.
abstract: Kisspeptin, neurokinin B (NKB) and dynorphin A (Dyn) are coexpressed within KNDy neurons that project from the hypothalamic arcuate nucleus (ARC) to GnRH neurons and numerous other hypothalamic targets. Each of the KNDy neuropeptides has been implicated in regulating pulsatile GnRH/LH secretion. In isolation, kisspeptin is generally known to stimulate, and Dyn to inhibit LH secretion. However, the NKB analog, senktide, has variously been reported to inhibit, stimulate or have no effect on LH secretion. In prepubertal mice, rats and monkeys, senktide stimulates LH secretion. Furthermore, in the monkey this effect is dependent on kisspeptin signaling through its receptor, GPR54. The present study tested the hypotheses that the stimulatory effects of NKB on LH secretion in intact rats are mediated by kisspeptin/GPR54 signaling and are independent of a Dyn tone. To test this, ovarian-intact prepubertal rats were subjected to frequent automated blood sampling before and after intracerebroventricular injections of KNDy neuropeptide analogs. Senktide robustly induced single LH pulses, while neither the GPR54 antagonist, Kp-234, nor the Dyn agonist and antagonist (U50488 and nor-BNI, respectively) had an effect on basal LH levels. However, Kp-234 potently blocked the senktide-induced LH pulses. Modulation of the Dyn tone by U50488 or nor-BNI did not affect the senktide-induced LH pulses. These data demonstrate that the stimulatory effect of NKB on LH secretion in intact female rats is dependent upon kisspeptin/GPR54 signaling, but not on Dyn signaling.
authors.affiliation: Division of Women's Health, School of Medicine, King's College London, London, United Kingdom.
authors.forename: Pasha
authors.initials: P
authors.lastname: Grachev
authors.forename: Xiao Feng
authors.initials: XF
authors.lastname: Li
authors.forename: Yuan Shao
authors.initials: YS
authors.lastname: Lin
authors.forename: Ming Han
authors.initials: MH
authors.lastname: Hu
authors.forename: Leena
authors.initials: L
authors.lastname: Elsamani
authors.forename: Stewart J
authors.initials: SJ
authors.lastname: Paterson
authors.forename: Robert P
authors.initials: RP
authors.lastname: Millar
authors.forename: Stafford L
authors.initials: SL
authors.lastname: Lightman
authors.forename: Kevin T
authors.initials: KT
authors.lastname: O'Byrne
mesh_terms: D019900:3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer / Q000494:pharmacology; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology; D012634:Bodily Secretions / Q000187:drug effects; D005260:Female; D059648:Kisspeptins / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D009271:Naltrexone / Q000031:analogs & derivatives / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D015287:Neurokinin B / Q000031:analogs & derivatives / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D043562:Receptors, G-Protein-Coupled / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000074502:Receptors, Kisspeptin-1; D018039:Receptors, Tachykinin / Q000819:agonists / Q000378:metabolism; D013373:Substance P / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000959:Antihypertensive Agents; C492247:Kiss1r protein, rat; D059648:Kisspeptins; D009292:Narcotic Antagonists; D010446:Peptide Fragments; D043562:Receptors, G-Protein-Coupled; D000074502:Receptors, Kisspeptin-1; D018039:Receptors, Tachykinin; C054724:senktide; D013373:Substance P; C051844:norbinaltorphimine; D009271:Naltrexone; D019900:3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer; D015287:Neurokinin B; D007986:Luteinizing Hormone