title: Methods for measuring DNA adducts and abasic sites I: isolation, purification, and analysis of DNA adducts in intact DNA.
abstract: The major event involved in the formation of mutations and the initiation and progression of cancer is the induction of DNA damage by reactive intermediates arising from exposure to endogenous and exogenous chemicals. Many electrophilic metabolites of chemicals covalently bind to the bases of DNA causing specific DNA adducts. This unit includes protocols for preparing samples of intact DNA and adduct analysis to quantify the number of adducts that can potentially cause mutagenic or carcinogenic damage.
authors.affiliation: University of North Carolina, Chapel Hill, NC, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Swenberg
authors.forename: Amy-Joan L
authors.initials: AJ
authors.lastname: Ham
authors.forename: Kevin S
authors.initials: KS
authors.lastname: McDorman
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Morinello
authors.forename: Jun
authors.initials: J
authors.lastname: Nakamura
authors.forename: Robert
authors.initials: R
authors.lastname: Schoonhoven
mesh_terms: D002460:Cell Line; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans
chemical_list: D018736:DNA Adducts; D004247:DNA