title: Compact zwitterion-coated iron oxide nanoparticles for in vitro and in vivo imaging.
abstract: We have recently developed compact and water-soluble zwitterionic dopamine sulfonate (ZDS) ligand coated superparamagnetic iron oxide nanoparticles (SPIONs) for use in various biomedical applications. The defining characteristics of ZDS-coated SPIONs are small hydrodynamic diameters, low non-specific interactions with fetal bovine serum, the opportunity for specific labeling, and stability with respect to time, pH, and salinity. We report here on the magnetic characterization of ZDS-coated SPIONs and their in vitro and in vivo performance relative to non-specific interactions with HeLa cells and in mice, respectively. ZDS-coated SPIONs retained the superparamagnetism and saturation magnetization (M(s)) of as-synthesized hydrophobic SPIONs, with M(s) = 74 emu g(-1) [Fe]. Moreover, ZDS-coated SPIONs showed only small non-specific uptake into HeLa cancer cells in vitro and low non-specific binding to serum proteins in vivo in mice.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
authors.forename: He
authors.initials: H
authors.lastname: Wei
authors.forename: Oliver T
authors.initials: OT
authors.lastname: Bruns
authors.forename: Ou
authors.initials: O
authors.lastname: Chen
authors.forename: Moungi G
authors.initials: MG
authors.lastname: Bawendi
mesh_terms: D000818:Animals; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis; D003287:Contrast Media / Q000138:chemical synthesis / Q000493:pharmacokinetics; D003911:Dextrans / Q000737:chemistry; D004298:Dopamine / Q000737:chemistry / Q000493:pharmacokinetics*; D004355:Drug Stability; D006367:HeLa Cells; D006801:Humans; D007477:Ions; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008968:Molecular Conformation; D057054:Molecular Imaging / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D020099:Coated Materials, Biocompatible; D003287:Contrast Media; D003911:Dextrans; D007477:Ions; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10; D004298:Dopamine