title: Protein encapsulation within synthetic molecular hosts.
abstract: Protein encapsulation has long attracted many chemists and biologists because of its potential to control the structure and functions of proteins, but has been a daunting challenge because of their incommensurably larger size compared with common synthetic hosts. Here we report the encapsulation of a small protein, ubiquitin, within giant coordination cages. The protein was attached to one bidentate ligand and, upon addition of Pd(II) ions (M) and additional ligands (L), M(12)L(24) coordination nanocages self-assembled around the protein. Because of the well-defined host framework, the protein-encapsulated structure could be analysed by NMR spectroscopy, ultracentrifugation and X-ray crystallography.
authors.affiliation: Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Daishi
authors.initials: D
authors.lastname: Fujita
authors.forename: Kosuke
authors.initials: K
authors.lastname: Suzuki
authors.forename: Sota
authors.initials: S
authors.lastname: Sato
authors.forename: Maho
authors.initials: M
authors.lastname: Yagi-Utsumi
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Mizuno
authors.forename: Takashi
authors.initials: T
authors.lastname: Kumasaka
authors.forename: Masaki
authors.initials: M
authors.lastname: Takata
authors.forename: Masanori
authors.initials: M
authors.lastname: Noda
authors.forename: Susumu
authors.initials: S
authors.lastname: Uchiyama
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujita
mesh_terms: D018360:Crystallography, X-Ray; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010165:Palladium / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D014461:Ultracentrifugation
chemical_list: D008024:Ligands; D011506:Proteins; D010165:Palladium