title: On-cell MAS NMR: physiological clues from living cells.
abstract: While structural information on biomolecules is mainly obtained from purified in vitro samples, NMR can also be applied in the context of entire cells or organisms. The present study describes maturation processes in living Salmonella enterica serovar Typhimurium, a prevalent cause for human gastroenteritis. In our physiological study, we follow the composition of the O-antigen on the outer bacterial membrane with high-resolution MAS NMR spectroscopy. We detect and characterize an evolution of the O-antigen composition, in particular of the O-acetylation state of the O-antigen, a factor that can play an important role in vaccine development.
authors.affiliation: Physical Chemistry, ETH Zurich, Wolfgang-Pauli Str. 10, 8093 Zurich, Switzerland.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Zandomeneghi
authors.forename: Karin
authors.initials: K
authors.lastname: Ilg
authors.forename: Markus
authors.initials: M
authors.lastname: Aebi
authors.forename: Beat H
authors.initials: BH
authors.lastname: Meier
mesh_terms: D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000737:chemistry; D002627:Chemistry, Physical; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens / Q000737:chemistry*; D012486:Salmonella typhimurium / Q000737:chemistry* / Q000166:cytology
chemical_list: D019081:O Antigens