title: Hepatocyte growth factor plays a key role in insulin resistance-associated compensatory mechanisms.
abstract: Insulin resistance is present in obesity and in type 2 diabetes and is associated with islet cell hyperplasia and hyperinsulinemia, but the driving forces behind this compensatory mechanism are incompletely understood. Previous data have suggested the involvement of an unknown circulating insulin resistance-related β-cell growth factor. In this context, looking for candidates to be a circulating factor, we realized that hepatocyte growth factor (HGF) is a strong candidate as a link between insulin resistance and increased mass of islets/hyperinsulinemia. Our approach aimed to show a possible cause-effect relationship between increase in circulating HGF levels and compensatory islet hyperplasia/hyperinsulinemia by showing the strength of the association, whether or not is a dose-dependent response, the temporality, consistency, plausibility, and reversibility of the association. In this regard, our data showed: 1) a strong and consistent correlation between HGF and the compensatory mechanism in three animal models of insulin resistance; 2) HGF increases β-cell mass in a dose-dependent manner; 3) blocking HGF shuts down the compensatory mechanisms; and 4) an increase in HGF levels seems to precede the compensatory response associated with insulin resistance, indicating that these events occur in a sequential mode. Additionally, blockages of HGF receptor (Met) worsen the impaired insulin-induced insulin signaling in liver of diet-induced obesity rats. Overall, our data indicate that HGF is a growth factor playing a key role in islet mass increase and hyperinsulinemia in diet-induced obesity rats and suggest that the HGF-Met axis may have a role on insulin signaling in the liver.
authors.affiliation: Department of Internal Medicine, State University of Campinas, Campinas, 13081-970 São Paulo, Brazil.
authors.forename: Tiago G
authors.initials: TG
authors.lastname: Araújo
authors.forename: Alexandre G
authors.initials: AG
authors.lastname: Oliveira
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Carvalho
authors.forename: Dioze
authors.initials: D
authors.lastname: Guadagnini
authors.forename: André O P
authors.initials: AO
authors.lastname: Protzek
authors.forename: Jose B C
authors.initials: JB
authors.lastname: Carvalheira
authors.forename: Antonio C
authors.initials: AC
authors.lastname: Boschero
authors.forename: Mario J A
authors.initials: MJ
authors.lastname: Saad
mesh_terms: D000821:Animal Feed; D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D004032:Diet; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D050417:Insulin-Secreting Cells / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D009765:Obesity / Q000378:metabolism; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D007328:Insulin; D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met