title: Click and pick: identification of sialoside analogues for siglec-based cell targeting.
abstract: Click 'n' chips: Azide and alkyne-bearing sialic acids (purple diamond; see picture) were subjected to high-throughput click chemistry to generate a library of sialic acid analogues. Microarray printing of the library and screening with the siglec family of sialic-acid-binding proteins, led to the identification of high-affinity ligands for siglec-9 and siglec-10.
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Cory D
authors.initials: CD
authors.lastname: Rillahan
authors.forename: Erik
authors.initials: E
authors.lastname: Schwartz
authors.forename: Ryan
authors.initials: R
authors.lastname: McBride
authors.forename: Valery V
authors.initials: VV
authors.lastname: Fokin
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
mesh_terms: D000818:Animals; D016466:CHO Cells; D002236:Carbohydrate Conformation; D002460:Cell Line; D057930:Click Chemistry; D006224:Cricetinae; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D051379:Mice; D019158:N-Acetylneuraminic Acid / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry*; D040081:Protein Array Analysis; D063265:Sialic Acid Binding Immunoglobulin-like Lectins / Q000737:chemistry*
chemical_list: D063265:Sialic Acid Binding Immunoglobulin-like Lectins; D019158:N-Acetylneuraminic Acid