title: In vitro methods for detecting cytotoxicity.
abstract: Toxicant-induced damage to cells (cytotoxicity) can depress cell growth, compromise intracellular metabolic processes, and/or cause loss of cell viability. Methods that indicate these cytotoxic changes following toxicant exposures are provided, including [³H]thymidine uptake to measure cell growth, MTT dye conversion to detect changes in metabolic activity, and trypan blue uptake to indicate loss of cell viability.
authors.affiliation: Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, Virginia, USA.
authors.forename: M
authors.initials: M
authors.lastname: Ehrich
authors.forename: L
authors.initials: L
authors.lastname: Sharova
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002477:Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D004396:Coloring Agents / Q000737:chemistry / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects; D006801:Humans; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D004396:Coloring Agents; D015262:Xenobiotics