title: Collagen-based layer-by-layer coating on electrospun polymer scaffolds.
abstract: Preparation of microfibre constructs of collagen by electrospinning has been problematic due to the instability of collagen in volatile solvents, such as 1,1,1,3,3,3-hexafluoro-2-propanol, so that electrospinning leads to a substantial amount of gelatin fibres. In the present study we have demonstrated the production of collagen-based microfibre constructs by use of a layer-by-layer coating process onto a preformed synthetic polymer microfibre base. Soluble native collagen, which has a basic isoelectric point, has been used with modified triple-helical collagens that have acidic isoelectric points. These modified collagens have been prepared as deamidated, succinylated, maleylated and citraconylated derivatives. Together, the acidic and basic collagens have successfully coated polyacrylonitrile and poly(DL-lactide-co-glycolide) fibres, as shown by spectroscopy and microscopy. These coatings allow good cell attachment and spreading on the fibres. The native, triple helical form of the collagen has been confirmed through use of a conformation dependent monoclonal antibody.
authors.affiliation: CSIRO Materials Science and Engineering, Bayview Avenue, Clayton, VIC 3169, Australia.
authors.forename: Yen B
authors.initials: YB
authors.lastname: Truong
authors.forename: Veronica
authors.initials: V
authors.lastname: Glattauer
authors.forename: Kelsey L
authors.initials: KL
authors.lastname: Briggs
authors.forename: Stefan
authors.initials: S
authors.lastname: Zappe
authors.forename: John A M
authors.initials: JA
authors.lastname: Ramshaw
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D003094:Collagen / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D011108:Polymers; C010504:polyacrylonitrile; D011100:Polyglycolic Acid; D003094:Collagen