title: Doppler optical coherence tomography of retinal circulation.
abstract: Noncontact retinal blood flow measurements are performed with a Fourier domain optical coherence tomography (OCT) system using a circumpapillary double circular scan (CDCS) that scans around the optic nerve head at 3.40 mm and 3.75 mm diameters. The double concentric circles are performed 6 times consecutively over 2 sec. The CDCS scan is saved with Doppler shift information from which flow can be calculated. The standard clinical protocol calls for 3 CDCS scans made with the OCT beam passing through the superonasal edge of the pupil and 3 CDCS scan through the inferonal pupil. This double-angle protocol ensures that acceptable Doppler angle is obtained on each retinal branch vessel in at least 1 scan. The CDCS scan data, a 3-dimensional volumetric OCT scan of the optic disc scan, and a color photograph of the optic disc are used together to obtain retinal blood flow measurement on an eye. We have developed a blood flow measurement software called "Doppler optical coherence tomography of retinal circulation" (DOCTORC). This semi-automated software is used to measure total retinal blood flow, vessel cross section area, and average blood velocity. The flow of each vessel is calculated from the Doppler shift in the vessel cross-sectional area and the Doppler angle between the vessel and the OCT beam. Total retinal blood flow measurement is summed from the veins around the optic disc. The results obtained at our Doppler OCT reading center showed good reproducibility between graders and methods (<10%). Total retinal blood flow could be useful in the management of glaucoma, other retinal diseases, and retinal diseases. In glaucoma patients, OCT retinal blood flow measurement was highly correlated with visual field loss (R(2)>0.57 with visual field pattern deviation). Doppler OCT is a new method to perform rapid, noncontact, and repeatable measurement of total retinal blood flow using widely available Fourier-domain OCT instrumentation. This new technology may improve the practicality of making these measurements in clinical studies and routine clinical practice.
authors.affiliation: Department of Ophthalmology, Oregon Health and Science University, OR, USA.
authors.forename: Ou
authors.initials: O
authors.lastname: Tan
authors.forename: Yimin
authors.initials: Y
authors.lastname: Wang
authors.forename: Ranjith K
authors.initials: RK
authors.lastname: Konduru
authors.forename: Xinbo
authors.initials: X
authors.lastname: Zhang
authors.forename: SriniVas R
authors.initials: SR
authors.lastname: Sadda
authors.forename: David
authors.initials: D
authors.lastname: Huang
mesh_terms: D001783:Blood Flow Velocity / Q000502:physiology; D005583:Fourier Analysis; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D012161:Retinal Artery / Q000033:anatomy & histology*; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods*