title: Coenzyme A and coenzyme A-glutathione mixed disulfide measurements by HPLC.
abstract: This unit describes a method for measuring coenzyme A and coenzyme A-glutathione mixed disulfide in tissue homogenates obtained from tissues frozen in liquid nitrogen, homogenized in the presence of N-ethylmaleimide, and then acidified. to avoid the oxidation, reduction, and thiol exchange reactions that can occur during tissue processing and isolation of subcellular fractions. This method may provide a way to assess changes in the oxidative state of mitochondria in intact tissues.
authors.affiliation: Children's Research Institute, The Ohio State University, Columbus, OH, USA.
authors.forename: Lynette K
authors.initials: LK
authors.lastname: Rogers
authors.forename: Charles V
authors.initials: CV
authors.lastname: Smith
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003065:Coenzyme A / Q000378:metabolism*; D004220:Disulfides / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*
chemical_list: D004220:Disulfides; D005978:Glutathione; D003065:Coenzyme A