title: Resveratrol preconditioning increases methionine sulfoxide reductases A expression and enhances resistance of human neuroblastoma cells to neurotoxins.
abstract: Methionine sulfoxide reductases A (MsrA) has been postulated to act as a catalytic antioxidant system involved in the protection of oxidative stress-induced cell injury. Recently, attention has turned to MsrA in coupling with the pathology of Parkinson's disease, which is closely related to neurotoxins that cause dopaminergic neuron degeneration. Here, we firstly provided evidence that pretreatment with a natural polyphenol resveratrol (RSV) up-regulated the expression of MsrA in human neuroblastoma SH-SY5Y cells. It was also observed that the expression and nuclear translocation of forkhead box group O 3a (FOXO3a), a transcription factor that activates the human MsrA promoter, increased after RSV pretreatment. Nicotinamide , an inhibitor of silent information regulator 1 (SIRT1), prevented RSV-induced elevation of FOXO3a and MsrA expression, indicating that the effect of RSV was mediated by a SIRT1-dependent pathway. RSV preconditioning increased methionine sulfoxide(MetO)-reducing activity in SH-SY5Y cells and enhanced their resistance to neurotoxins, including chloramine-T and 1-methyl-4-phenyl-pyridinium. In addition, the enhancement of cell resistance to neurotoxins caused by RSV preconditioning can be largely prevented by MsrA inhibitor dimethyl sulfoxide. Our findings suggest that treatment with polyphenols such as RSV can be used as a potential regulatory strategy for MsrA expression and function.
authors.affiliation: Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Wu
authors.forename: Na
authors.initials: N
authors.lastname: Xie
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Zhang
authors.forename: Xin-Lei
authors.initials: XL
authors.lastname: Guan
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Long
authors.forename: Yuan-Long
authors.initials: YL
authors.lastname: Li
authors.forename: Qiu-Ju
authors.initials: QJ
authors.lastname: Xiong
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zeng
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Chen
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002700:Chloramines / Q000633:toxicity*; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008715:Methionine / Q000031:analogs & derivatives / Q000494:pharmacology; D051150:Methionine Sulfoxide Reductases / Q000235:genetics* / Q000378:metabolism; D008954:Models, Biological; D009447:Neuroblastoma; D009498:Neurotoxins / Q000494:pharmacology; D018384:Oxidative Stress; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*; D014105:Tosyl Compounds / Q000633:toxicity*; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D002700:Chloramines; C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D009498:Neurotoxins; D013267:Stilbenes; D014105:Tosyl Compounds; C016300:chloramine-T; D008715:Methionine; D051150:Methionine Sulfoxide Reductases; C027219:methionine sulfoxide reductase; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D000077185:Resveratrol; D015655:1-Methyl-4-phenylpyridinium; C013111:methionine sulfoxide