title: Immune-enhancing activity comparison of sulfated ophiopogonpolysaccharide and sulfated jujube polysaccharide.
abstract: The immune-enhancing activities of four sulfated polysaccharides, sOPS(t), sOPS(80), sJPS(t) and sJPS(50) picked out in our previous researches, were compared taking four corresponding unmodified polysaccharides as control. In vitro experiment, the effects of eight polysaccharides on chicken peripheral lymphocyte proliferation were determined by MTT assay. The result displayed that four sulfated polysaccharides could significantly stimulate lymphocyte proliferation, their actions were significantly or numerically stronger than those of corresponding unmodified polysaccharides, sOPS(80) presented better efficacy. In vivo experiment, 300 14-day-old chickens were averagely divided into ten groups. The chickens except blank control (BC) group were vaccinated with Newcastle disease vaccine, repeated vaccination at 28 days old. At the same time of the first vaccination, the chickens in eight polysaccharides groups were injected respectively with 0.5 ml (1mg) of eight polysaccharides, in vaccination control (VC) and BC group, with 0.5 mL of physiological saline, once a day for three successive days. On days 7, 14, 21 and 28 after the first vaccination, the peripheral lymphocyte proliferation and serum ND antibody titer were determined. The result showed that four sulfated polysaccharides could significantly promote lymphocyte proliferation and enhance serum antibody titer. Their actions were significantly or numerically stronger than those of corresponding unmodified polysaccharides, sOPS(80) possessed the best efficacy. These results indicated that sulfation modification could enhance the immune-enhancing activity of OPS and JPS, sOPS(80) possessed the best efficacy and would be expected as a component drug of new-type immunopotentiator.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.forename: Cunshuai
authors.initials: C
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Qin
authors.forename: Cui
authors.initials: C
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Sheng
authors.forename: The Luong
authors.initials: TL
authors.lastname: Nguyen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens / Q000276:immunology*; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology*; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control; D009522:Newcastle disease virus / Q000276:immunology; D031441:Ophiopogon / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D013463:Sulfuric Acid Esters / Q000737:chemistry / Q000494:pharmacology; D014611:Vaccination; D014765:Viral Vaccines / Q000276:immunology / Q000494:pharmacology; D031957:Ziziphus / Q000737:chemistry*
chemical_list: D000914:Antibodies, Viral; D007155:Immunologic Factors; D011134:Polysaccharides; D013463:Sulfuric Acid Esters; D014765:Viral Vaccines