title: CRP detection from serum for chip-based point-of-care testing system.
abstract: Most of point-of-care testing (POCT) to improve facilitates in diagnosis, treatment, and monitoring of patients. POCT technique has still remained a quantitatively and accurately detective effect. In this article, we demonstrated that real human C-reactive protein (CRP) in serum was detected for a chip-based point-of-care testing application based on a nanogap-embedded field effect transistor (FET), and the results were compared with those obtained via the enzyme-linked immunosorbent assay (ELISA) method. The limit of detection (LOD), determined from the standard curve, was 0.1 ng/ml, which is comparable to that of commercialized ELISAs. We evaluated that an improved detection range (0.1 ng/ml to 100 ng/ml) was achieved by comparing with commercialized ELISA. Control experiments to determine selectivity and to discern false-positive/false-negative rates were also performed. This report is the first description of the detection of CRP in human serum using a silicon-based biosensor.
authors.affiliation: Department of Electrical Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Chang-Hoon
authors.initials: CH
authors.lastname: Kim
authors.forename: Jae-Hyuk
authors.initials: JH
authors.lastname: Ahn
authors.forename: Jee-Yeon
authors.initials: JY
authors.lastname: Kim
authors.forename: Ji-Min
authors.initials: JM
authors.lastname: Choi
authors.forename: Kyung-Choon
authors.initials: KC
authors.lastname: Lim
authors.forename: Tae Jung
authors.initials: TJ
authors.lastname: Park
authors.forename: Nam Su
authors.initials: NS
authors.lastname: Heo
authors.forename: Hee Gu
authors.initials: HG
authors.lastname: Lee
authors.forename: Jong-Wan
authors.initials: JW
authors.lastname: Kim
authors.forename: Yang-Kyu
authors.initials: YK
authors.lastname: Choi
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D002097:C-Reactive Protein / Q000032:analysis*; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014173:Transistors, Electronic
chemical_list: D002097:C-Reactive Protein