title: Development and characterization of 110 novel EST-SSR markers for Dendrobium officinale (Orchidaceae).
abstract: OBJECTIVE
  Expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers were developed in Dendrobium officinale by screening a cDNA library. The loci were verified by sequencing and explored for polymorphism among 19 genotypes and transferability among 30 other distantly related Dendrobium species. •
  
  
  RESULTS
  One hundred ten EST-SSRs were developed, and a set of 20 amplified two to six nucleotide repeats with a mean number of 2.5 alleles per locus and with an observed heterozygosity and polymorphism information content per locus ranging from 0.3463 to 0.9003 and 0.0997 to 0.6537 in 19 D. officinale genotypes, respectively. Furthermore, 92 of these markers have cross-taxa transferability, ranging from 6.45% to 100% among 30 other distantly related Dendrobium species. •
  
  
  CONCLUSIONS
  The developed markers have potential for application in germplasm appraisal, genetic diversity study, genetic mapping, and molecular breeding in D. officinale and other congeneric species.
authors.affiliation: Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 310018, People's Republic of China.
authors.forename: Jiang-Jie
authors.initials: JJ
authors.lastname: Lu
authors.forename: Na-Na
authors.initials: NN
authors.lastname: Suo
authors.forename: Xu
authors.initials: X
authors.lastname: Hu
authors.forename: Shang
authors.initials: S
authors.lastname: Wang
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Liu
authors.forename: Hui-Zhong
authors.initials: HZ
authors.lastname: Wang
mesh_terms: D031667:Dendrobium / Q000235:genetics*; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data
chemical_list: D005819:Genetic Markers