title: Validation of a method for simultaneous determination of nitroimidazoles, benzimidazoles and chloramphenicols in swine tissues by ultra-high performance liquid chromatography-tandem mass spectrometry.
abstract: This paper presents a sensitive and confirmatory multi-residue method for the analysis of 23 veterinary drugs and metabolites belonging to three classes (nitroimidazoles, benzimidazoles, and chloramphenicols) in porcine muscle, liver, and kidney. After extracted with ethyl acetate and basic ethyl acetate sequentially, the crude extracts were defatted with hexane and further purified using Oasis MCX solid-phase extraction cartridges. Rapid determination was carried out by ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry. Data acquisition was performed under positive and negative mode simultaneously. Recoveries based on matrix-matched calibrations for meat, liver, and kidney ranged from 50.6 to 108.1%. The method quantification limits were in the range of 3-100ng/kg.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Xia
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Zhong
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Li
authors.forename: Yaoling
authors.initials: Y
authors.lastname: Huang
authors.forename: Shuangyang
authors.initials: S
authors.lastname: Ding
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000378:metabolism; D001562:Benzimidazoles / Q000032:analysis* / Q000378:metabolism; D002701:Chloramphenicol / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007668:Kidney / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D009132:Muscles / Q000737:chemistry / Q000378:metabolism; D009593:Nitroimidazoles / Q000032:analysis* / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods; D019155:Veterinary Drugs / Q000032:analysis* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001562:Benzimidazoles; D009593:Nitroimidazoles; D019155:Veterinary Drugs; D002701:Chloramphenicol