title: Tissue specific delivery of estrone-conjugated siRNAs.
abstract: An estrone phosphoramidite, synthesized in a single step, was directly incorporated at the 5' ends of small interfering RNAs (siRNAs). The resulting estrone-conjugated siRNAs readily pass through cellular membranes and down-regulate the target protein, and show specific distributions in vivo.
authors.affiliation: Department of Chemistry, BK School of Molecular Science, Pohang University of Science and Technology, Pohang 790-784, Korea.
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Bang
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Jeon
authors.forename: Wanil
authors.initials: W
authors.lastname: Kim
authors.forename: Kyung-Ha
authors.initials: KH
authors.lastname: Lee
authors.forename: Kyong-Tai
authors.initials: KT
authors.lastname: Kim
authors.forename: Byeang Hyean
authors.initials: BH
authors.lastname: Kim
mesh_terms: D000818:Animals; D004970:Estrone / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques / Q000379:methods; D018014:Gene Transfer Techniques; D006801:Humans; D034904:Lamin Type A / Q000235:genetics / Q000378:metabolism; D061986:MCF-7 Cells; D051379:Mice; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence; D008956:Models, Chemical; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000493:pharmacokinetics; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014018:Tissue Distribution; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D034904:Lamin Type A; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; D034741:RNA, Small Interfering; D042461:Vascular Endothelial Growth Factor A; C434331:phosphoramidite; D004970:Estrone; D016650:Fluorescein-5-isothiocyanate