title: Isotope-based analysis of modified tRNA nucleosides correlates modification density with translational efficiency.
abstract: Useful diversity: Quantification of modified tRNA nucleobases in different murine and porcine tissues reveals a tissue-specific overall modification content. The modification content correlates with rates of protein synthesis in vitro, suggesting a direct link between tRNA modification levels and tissue-specific translational efficiency.
authors.affiliation: Center for Integrated Protein Science at the Department of Chemistry, Ludwig-Maximilians-Universität München, Butenandtstrasse 5-13, 81377 Munich, Germany.
authors.forename: Caterina
authors.initials: C
authors.lastname: Brandmayr
authors.forename: Mirko
authors.initials: M
authors.lastname: Wagner
authors.forename: Tobias
authors.initials: T
authors.lastname: Brückl
authors.forename: Daniel
authors.initials: D
authors.lastname: Globisch
authors.forename: David
authors.initials: D
authors.lastname: Pearson
authors.forename: Andrea Christa
authors.initials: AC
authors.lastname: Kneuttinger
authors.forename: Veronika
authors.initials: V
authors.lastname: Reiter
authors.forename: Antje
authors.initials: A
authors.lastname: Hienzsch
authors.forename: Susanne
authors.initials: S
authors.lastname: Koch
authors.forename: Ines
authors.initials: I
authors.lastname: Thoma
authors.forename: Peter
authors.initials: P
authors.lastname: Thumbs
authors.forename: Stylianos
authors.initials: S
authors.lastname: Michalakis
authors.forename: Markus
authors.initials: M
authors.lastname: Müller
authors.forename: Martin
authors.initials: M
authors.lastname: Biel
authors.forename: Thomas
authors.initials: T
authors.lastname: Carell
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D002853:Chromatography, Liquid; D003903:Deuterium / Q000737:chemistry*; D007553:Isotope Labeling; D013058:Mass Spectrometry; D051379:Mice; D009587:Nitrogen Isotopes; D009705:Nucleosides / Q000032:analysis* / Q000378:metabolism; D012343:RNA, Transfer / Q000032:analysis* / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D013552:Swine
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D009705:Nucleosides; D012343:RNA, Transfer; D003903:Deuterium; D012259:Ribonuclease, Pancreatic