title: Tinospinosides D, E, and tinospin E, further clerodane diterpenoids from Tinospora sagittata.
abstract: Chemical investigation on the roots of Tinospora sagittata resulted in the isolation of three novel cis-clerodane diterpenoids, tinospinosides D, E, and tinospin E, together with two known compounds, columbin and columbin glucoside, and their structures were determined by extensive spectroscopic analyses, chemical reactions and computer-assisted calculations. The inhibitory activity of the isolated compounds and their chemical derivatives on nitric oxide production in lipopolysaccharide and interferon-γ activited J774.1 macrophage-like cells was also evaluated.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Fenghua
authors.initials: F
authors.lastname: Ma
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Asada
authors.forename: Kazuo
authors.initials: K
authors.lastname: Koike
mesh_terms: D000818:Animals; D002460:Cell Line; D045785:Diterpenes, Clerodane / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D007371:Interferon-gamma / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008958:Models, Molecular; D009569:Nitric Oxide / Q000276:immunology; D018517:Plant Roots / Q000737:chemistry*; D031610:Tinospora / Q000737:chemistry*
chemical_list: D045785:Diterpenes, Clerodane; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D007371:Interferon-gamma