title: Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides.
abstract: BACKGROUND
  Sesamia nonagrioides is a noctuid that feeds on maize, sugar cane and sorghum in North Africa and Southern Europe. Larvae reared under long day conditions pupate after 5 or 6 larval instars, whereas larvae reared under short day conditions enter diapause and undergo up to 12 molts before dying or pupating. To better understand the mechanism of larval development and diapause, we identified proteins with different expressions in the sixth instar of diapausing and non-diapausing larvae.
  
  
  RESULTS
  A total of 52 differentially regulated proteins were detected in the hemolymph of the diapausing or non-diapausing larvae at the beginning or end of the sixth instar. From these proteins, 11 were identified by mass spectrometry (MALDI-TOF MS or MALDI-TOF/TOF MS/MS): 5 were upregulated in the hemolymph of non-diapausing larvae and 6 in the hemolymph of the diapausing larvae. Interestingly, some proteins were expressed only in non-diapausing larvae but none was expressed only in the hemolymph of diapausing larvae. The possible functions of some of these proteins related to diapause maintenance or to larval-pupal metamorphosis are discussed.
  
  
  CONCLUSIONS
  The 2-DE proteomic map of S. nonagrioides hemolymph shows differential protein expression in diapausing and non-diapausing larvae. Some proteins that showed higher expression in the diapausing larvae at the end of the sixth instar could be involved in JH level maintenance thus in the diapause status maintenance. On the contrary, other proteins that showed the highest expression or that were expressed only in the non-diapausing larvae could be involved in larval-pupal metamorphosis.
authors.affiliation: Department of Crop and Forest Sciences, University of Lleida, AGROTECNIO Center, Rovira Roure 191, Lleida, 25198, Spain. Eizaguirre@pvcf.udl.cat.
authors.forename: Meritxell
authors.initials: M
authors.lastname: Pérez-Hedo
authors.forename: Isabel
authors.initials: I
authors.lastname: Sánchez-López
authors.forename: Matilde
authors.initials: M
authors.lastname: Eizaguirre