title: [A new immunoassay for estradiol on the Advia-Centaur (Siemens) system: analytical and clinical performances].
abstract: The objective of this study is to present results for measurement of estradiol (E(2)) in serum and control materials with a new automated assay (eE(2)) that could be used in random continuous access mode on the Siemens laboratories Advia-Centaur immunoassay analyzer. We evaluated the imprecision (within and between run), the linearity, the limit of detection, and the functional sensitivity. We have tested the assay for monitoring ovulation stimulation for in vitro fertilization and embryo transfer (IVF-ET) throughout the most frequent protocol utilized in our IVF centre (n = 247). Results are compared to those obtained by the E(2)6 assay, one other assay on the Advia-Centaur immunoassay analyzer (Siemens), routinely used in our hormonology department. The obtained results show that the new assay presents better analytical performances (precision, linearity, limits of detection and sensibility) than the previous technique.
authors.affiliation: Service de biochimie et d'hormonologie, Hôpital Antoine Béclère, Clamart, France.
authors.forename: Mounia
authors.initials: M
authors.lastname: Lachgar
authors.forename: Guillaume
authors.initials: G
authors.lastname: Moulin
authors.forename: Joëlle
authors.initials: J
authors.lastname: Taieb
mesh_terms: D001322:Autoanalysis; D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods*; D003940:Diagnostic Techniques, Endocrine / Q000295:instrumentation; D004526:Efficiency; D004958:Estradiol / Q000032:analysis* / Q000097:blood; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D057230:Limit of Detection; D008593:Menopause / Q000097:blood; D008954:Models, Biological; D010060:Ovulation / Q000097:blood; D010062:Ovulation Induction; D015203:Reproducibility of Results; D027724:Reproductive Techniques, Assisted / Q000295:instrumentation; D012680:Sensitivity and Specificity
chemical_list: D004958:Estradiol