title: In-situ enrichment of phosphopeptides on MALDI plates modified by ambient ion landing.
abstract: We report substantial in-situ enrichment of phosphopeptides in peptide mixtures using titanium and zirconium dioxide-coated matrix assisted laser desorption-ionization (MALDI) plates prepared by recently reported ambient ion landing deposition technique. The technique was able to modify four common materials currently used for MALDI targets (stainless steel, aluminum, indium-tin oxide glass and polymeric anchor chip). The structure of the deposited dioxide was investigated by electron microscopy, and different surfaces were compared and discussed in this study. Two standard proteins were used to test the enrichment capabilities of modified MALDI plates: casein and in-vitro phosphorylated trehalase. The enrichment of casein tryptic digest resulted in identification of 20 phosphopeptides (including miscleavages). Trehalase was used as a suitable model of larger protein that provided more complex peptide mixture after the trypsin digestion. All four possible phosphorylation sites in trehalase were identified and up to seven phosphopetides were found (including methionine oxidations and miscleavages). Two different mass spectrometers, MALDI-Fourier transform ion cyclotron resonance (FTICR) and MALDI-time of flight, were used to detect the phosphopeptides from modified MALDI plates after the enrichment procedure. It was observed that the desorption-ionization phenomena on the modified surfaces are not critically influenced by the parameters of the different MALDI ion sources (e.g. different pressure, different extraction voltages), and thus the presence of dioxide layer on the standard MALDI plate does not significantly interfere with the main MALDI processes. The detection of phosphopeptides after the enrichment could be done by both instruments. Desorption electrospray ionization coupled to the FTICR was also tested, but, unlike MALDI, it did not provide satisfactory results.
authors.affiliation: Institute of Microbiology of the ASCR, vvi, Prague, Czech Republic.
authors.forename: Lukáš
authors.initials: L
authors.lastname: Krásný
authors.forename: Petr
authors.initials: P
authors.lastname: Pompach
authors.forename: Martin
authors.initials: M
authors.lastname: Strohalm
authors.forename: Veronika
authors.initials: V
authors.lastname: Obsilova
authors.forename: Marcela
authors.initials: M
authors.lastname: Strnadová
authors.forename: Petr
authors.initials: P
authors.lastname: Novák
authors.forename: Michael
authors.initials: M
authors.lastname: Volný
mesh_terms: D000595:Amino Acid Sequence; D002364:Caseins / Q000737:chemistry; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010748:Phosphopeptides / Q000737:chemistry* / Q000302:isolation & purification; D010766:Phosphorylation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*; D014198:Trehalase / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry
chemical_list: D002364:Caseins; D010446:Peptide Fragments; D010748:Phosphopeptides; D015040:Zirconium; D014198:Trehalase; D014357:Trypsin; C028541:zirconium oxide