title: Photo-killing mechanism of 2-demethoxy-2,3-ethylenediamino hypocrellin B (EDAHB) to HeLa cells.
abstract: 2-Demethoxy-2,3-ethylenediamino hypocrellin B (EDAHB) is a diamino-substituted hypocrellin B (HB) with high absorption of red light and high quantum yield of both singlet oxygen ((1)O(2)) and superoxide anions (O(2)(-)). Here we reported the cellular uptake, subcellular location, and cytotoxicity of EDAHB, as well as EDAHB-mediated photodynamic therapy (PDT) efficiency, and cell apoptosis. Results showed that EDAHB accumulated in HeLa cells rapidly up to 1h, with a subsequent decrease in the rate of uptake. EDAHB distributed with well-defined spots throughout the cytoplasm of the cells. EDAHB showed a much higher photopotentiation factor than HB. The phototoxicity of EDAHB to HeLa cells occurred via a mitochondria/caspase apoptosis pathway. This study showed EDAHB to be a promising candidate of photosensitizer for anti-tumor PDT.
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100124, China. zhouzhixiang@bjut.edu.cn
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zhou
authors.forename: QianQian
authors.initials: Q
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.forename: Hongtao
authors.initials: H
authors.lastname: Ma
authors.forename: Wang
authors.initials: W
authors.lastname: Sheng
authors.forename: Zelin
authors.initials: Z
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
authors.forename: Rugang
authors.initials: R
authors.lastname: Zhong
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001692:Biological Transport; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045304:Cytochromes c / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007928:Lethal Dose 50; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D010569:Perylene / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D017319:Photosensitizing Agents / Q000378:metabolism / Q000494:pharmacology*; D011809:Quinones / Q000378:metabolism / Q000494:pharmacology*; D011810:Quinoxalines / Q000378:metabolism / Q000494:pharmacology*
chemical_list: C581200:2-demethoxy-2,3-ethylenediamino hypocrellin B; D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D011809:Quinones; D011810:Quinoxalines; D010569:Perylene; D045304:Cytochromes c; D053148:Caspase 3; D053453:Caspase 9; C064522:hypocrellin B