title: Microsatellites grant more stable flanking genes.
abstract: BACKGROUND
  Microsatellites, or simple sequence repeats (SSRs), are DNA sequences that include tandem copies of specific sequences no longer than six bases. SSRs are ubiquitous in all genomes and highly mutable.
  
  
  OBJECTIVE
  Results from previous studies suggest that flanking regions of SSR are exhibit high stability in a wide range of organisms. We hypothesized that the SSRs ability to discard weak DNA polymerases could be responsible for this unusual stability. . When the weak polymerases are being decayed over SSRs, the flanking sequences would have higher opportunity to be replicated by more stable DNA polymerases. We present evidence of the molecular basis of our hypothesis.
  
  
  METHODS
  The hypothesis could be tested by examining the activity of DNA polymerase during and after a number of PCRs. The PCR reactions should be run with the same SSR locus possessing differences in the SSR length. The hypothesis could also be tested by comparing the mutational rate of a transferred gene between two transformations. The first one has a naked T-DNA (transferred DNA), while the second one has the same T-DNA flanked with two SSRs.
  
  
  CONCLUSIONS
  In any transformation experiment, flanking the T-DNA fragment with SSR sequences would result in more stably transferred genes. This process would decrease the unpredictable risks that may occur because of the mutational pressure on this foreign segment.
authors.affiliation: University of Aleppo, Aleppo, Syria.
authors.forename: Reem
authors.initials: R
authors.lastname: Joukhadar
authors.forename: Abdulqader
authors.initials: A
authors.lastname: Jighly
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism*; D005796:Genes / Q000235:genetics*; D016678:Genome / Q000235:genetics; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D008957:Models, Genetic; D009154:Mutation; D010838:Phytophthora / Q000235:genetics; D010944:Plants / Q000235:genetics; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary
chemical_list: D004269:DNA, Bacterial; C036483:T-DNA; D004247:DNA; D004259:DNA-Directed DNA Polymerase