title: AMT (3-(2-aminopropyl)indole) and 5-IT (5-(2-aminopropyl)indole): an analytical challenge and implications for forensic analysis.
abstract: 5-(2-Aminopropyl)indole (5-IT) and 3-(2-aminopropyl)indole (α-methyltryptamine, AMT) are isomeric substances and their differentiation can be a challenge under routine analytical conditions, especially when reference material is unavailable. 5-IT represents a very recent addition to the battery of new psychoactive substances that are commercially available from online retailers. This report illustrates how subtle differences observed under mass spectral and UV conditions can help to facilitate the differentiation between the two isomers. Analyses included (1)  H and (13) C NMR, GC-EI/CI ion trap MS, applications of several U/HPLC-DAD and HPLC-MS methods. Investigations currently underway also highlight the confirmation that AMT was detected in a number of fatal intoxications. These findings also demonstrate that there is a potential risk of misidentification when dealing with both substances.
authors.affiliation: ROAR Forensics, Malvern Hills Science Park, Geraldine Road, Malvern, WR14 3SZ, UK. simontox@yahoo.co.uk
authors.forename: Simon P
authors.initials: SP
authors.lastname: Elliott
authors.forename: Simon D
authors.initials: SD
authors.lastname: Brandt
authors.forename: Sally
authors.initials: S
authors.lastname: Freeman
authors.forename: Roland P
authors.initials: RP
authors.lastname: Archer
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005554:Forensic Medicine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007211:Indoles / Q000032:analysis / Q000097:blood*; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D011619:Psychotropic Drugs / Q000032:analysis / Q000097:blood*
chemical_list: C583010:3-(2-aminopropyl)indole; D007211:Indoles; D011619:Psychotropic Drugs; C583009:5-(2-aminopropyl)indole