title: Antibacterial activity of various honey types of Algeria against Staphylococcus aureus and Streptococcus pyogenes.
abstract: OBJECTIVE
  To assess the in vitro antibacterial activity of honey from different geographical location on Gram negative organisms.
  
  
  METHODS
  Different concentrations (Undiluted honey, 10 %, 30%, 50% and 70% wt/vol) of honey were studied in vitro using Staphylococcus aureus (S. aureus) and Streptococcus pyogenes (S. pyogenes), briefly, two-fold dilutions of honey solutions were tested to determine the minimum inhibitory concentration (MIC) against each type of microorganism, followed by more assays within a narrower dilution range to obtain more precise MIC values. MICs were determined by both visual inspection and spectrophotometric assay at 620 nm. These honey samples were compared with standard antibiotics like ampicillin, penicillin G, amoxicillin, gentamycin, tobramycin, erythromycin and chloramphenicol was determined by the disc diffusion method.
  
  
  RESULTS
  The diameter of zone of the inhibition (ZDI) of honey has various concentrations tested for the isolates ranged 0-46 mm for S. aureus, 0-44 mm for S. pyogenes. While the MIC (%) ranged 12%-95%, 25%-73% respectively.
  
  
  CONCLUSIONS
  Algeria honey, in-vitro, possess antibacterial activity.
authors.affiliation: Institute of Veterinary Sciences University Ibn-khadoun Tiaret, Algeria. moussa7014@yahoo.fr
authors.forename: Ahmed
authors.initials: A
authors.lastname: Moussa
authors.forename: Djebli
authors.initials: D
authors.lastname: Noureddine
authors.forename: Hammoudi Si
authors.initials: HS
authors.lastname: Mohamed
authors.forename: Meslem
authors.initials: M
authors.lastname: Abdelmelek
authors.forename: Aissat
authors.initials: A
authors.lastname: Saad
mesh_terms: D000462:Algeria / Q000453:epidemiology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D006722:Honey / Q000032:analysis; D006801:Humans; D008826:Microbial Sensitivity Tests; D013053:Spectrophotometry; D013203:Staphylococcal Infections / Q000188:drug therapy*; D013211:Staphylococcus aureus / Q000187:drug effects*; D013297:Streptococcus pyogenes / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents