title: Application of matrix-assisted laser desorption ionization-time of flight mass spectrometry for discrimination of laboratory-derived antibiotic-resistant bacteria.
abstract: The ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to discriminate laboratory-derived antibiotic-resistant bacterial strains of known genetic origin was examined. A computer-based cluster analysis of spectral data successfully discriminated the majority of single- as well as multiple-antibiotic-resistant Escherichia coli strains examined. Cluster analysis of Staphylococcus aureus strains with different levels of novobiocin resistance showed that as the degree of resistance increased similarity to the wild-type strain decreased. These results demonstrate that MALDI-TOF MS is capable of discriminating antibiotic-resistant bacterial strains and may have potential for differentiating bacterial strains with varying degrees of antibiotic-resistance.
authors.affiliation: Research Institute of Pharmaceutical Sciences, Musashino University, 1–1–20 Shinmachi, Nishitokyo, Tokyo 202–8585, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Muroi
authors.forename: Keisuke
authors.initials: K
authors.lastname: Shima
authors.forename: Masayuki
authors.initials: M
authors.lastname: Igarashi
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Nakagawa
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Tanamoto
mesh_terms: D000900:Anti-Bacterial Agents; D016000:Cluster Analysis; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013211:Staphylococcus aureus
chemical_list: D000900:Anti-Bacterial Agents