title: Interleukin-29 suppresses hepatitis A and C viral internal ribosomal entry site-mediated translation.
abstract: Our aim was to investigate the effects of interferons (IFNs)-λ (interleukin-29 [IL-29], IL-28A, and IL-28B) on hepatitis C virus (HCV) and hepatitis A virus (HAV) internal ribosomal entry site (IRES)-mediated translation. The effects of these IFNs on HCV/HAV translation from HAV/HCV IRES were investigated using bicistronic reporter constructs. We transfected HCV/HAV IRES constructs into these IFN-expressing cell lines. IL-29 showed stronger inhibition of their IRES-mediated translation. Combining IL-29 with IFN-α or amantadine resulted in stronger inhibition of HAV IRES activity. Our findings demonstrated a novel antiviral effect of IFNs-λ against HAV and HCV through the suppression of IRES-mediated translation.
authors.affiliation: Department of Medicine and Clinical Oncology, Chiba University Graduate School of Medicine, Chiba, Japan. kandat-cib@umin.ac.jp
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Kanda
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kiyohara
authors.forename: Shingo
authors.initials: S
authors.lastname: Nakamoto
authors.forename: Xia
authors.initials: X
authors.lastname: Jiang
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Miyamura
authors.forename: Fumio
authors.initials: F
authors.lastname: Imazeki
authors.forename: Koji
authors.initials: K
authors.lastname: Ishii
authors.forename: Takaji
authors.initials: T
authors.lastname: Wakita
authors.forename: Osamu
authors.initials: O
authors.lastname: Yokosuka
mesh_terms: D020121:5' Untranslated Regions; D000547:Amantadine / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D016174:Hepacivirus / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D030041:Hepatitis A virus / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007372:Interferons; D007378:Interleukins / Q000378:metabolism / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012270:Ribosomes / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects; D000095022:Interferon Lambda
chemical_list: D020121:5' Untranslated Regions; D000547:Amantadine; D000998:Antiviral Agents; D007371:Interferon-gamma; D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda