title: The effect of G-CSF on F-actin reorganization in HL-60 and K562 cell             lines.
abstract: The aim of this investigation was to show the influence of G-CSF (G-CSF)             on the F-actin cytoskeleton and the morphology of G-CSFR-proficient HL-60 and             G-CSFR-deficient K562 cell lines. In the present study, we show changes in F-actin             distribution in HL-60 cells after treatment with 5 and 10 ng/ml concentration             of G-CSF but also changes in the organization and fluorescence intensity of F-actin             in the K562 cell line. After treatment of HL-60 cells with 5 ng/ml concentration             of G-CSF we observed an increase in F-actin levels. Additionally, a higher labeling             of nuclear F-actin under TEM was observed. Moreover, changes in the cell cycle             indicate cell differentiation. On the other hand, in the K562 cell line we observed             an increase in the percentage sub-G1 cells following treatment with both concentration             of G-CSF. Furthermore, an increase in the percentage of late apoptotic cells after             G-CSF treatment was observed. A statistically significant difference in the cytoplasmic             F-actin levels was not detected, but nuclear levels were decreased. In conclusion,             we suggest that the G-CSF-based reorganization of actin filaments in HL-60 cells             is involved in the differentiation process. Moreover, we suggest that the G-CSF-induced             changes observed in K562 cells are associated with a G-CSF receptor-independent             pathway or its binding to other similar receptors.
authors.affiliation: Department of Histology and Embryology, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-092 Bydgoszcz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Izdebska
authors.forename: Dariusz
authors.initials: D
authors.lastname: Grzanka
authors.forename: Maciej
authors.initials: M
authors.lastname: Gagat
authors.forename: Lidia
authors.initials: L
authors.lastname: Gackowska
authors.forename: Alina
authors.initials: A
authors.lastname: Grzanka
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism* / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000378:metabolism
chemical_list: D000199:Actins; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D016179:Granulocyte Colony-Stimulating Factor