title: The role of the structural domains of human BST-2 in inhibiting the release of xenotropic murine leukemia virus-related virus.
abstract: BST-2 (bone marrow stromal cell antigen 2) is an interferon-inducible protein that inhibits the release of a variety of enveloped viruses by tethering viral particles to the cell surface. Xenotropic murine leukemia virus-related virus (XMRV) is a gamma-retrovirus that was derived from the recombination of two endogenous murine leukemia viruses during the production of a prostate cell line in mice. In this study, we observed that XMRV was highly sensitive to the inhibition by human BST-2. We were able to determine the structural domains of BST-2 that are essential to restrict XMRV, including the transmembrane domain, the coiled-coil ectodomain, the C-terminal glycosylphosphatidylinositol (GPI) anchor, the two putative N-linked glycosylation sites, and the three extracellular cysteine residues. Protease treatment effectively released XMRV particles into the supernatant, supporting the notion that BST-2 tethered nascent particles to the cell surface. These data suggest that BST-2 poses a strong restriction toward XMRV production.
authors.affiliation: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Hu
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Pang
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Shan
authors.initials: S
authors.lastname: Cen
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D002522:Chlorocebus aethiops; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D017434:Protein Structure, Tertiary; D012192:Retroviridae Infections / Q000378:metabolism* / Q000821:virology*; D014709:Vero Cells; D014779:Virus Replication; D058008:Xenotropic murine leukemia virus-related virus / Q000502:physiology*; D000074010:Bone Marrow Stromal Antigen 2
chemical_list: D015703:Antigens, CD; D058851:GPI-Linked Proteins; C094572:BST2 protein, human; D000074010:Bone Marrow Stromal Antigen 2