title: Determining the ability of xenobiotic metals to bind a specific protein domain by electrophoresis.
abstract: Environmental metals are potentially toxic and can interfere with the metal-binding motifs of various critical proteins. This unit describes an electrophoretic method that can be used to measure the ability of a xenobiotic metal to bind a zinc-finger motif. Information gained using this protocol can lead to the identification of protein targets of metals and shed better light on their mechanisms of action.
authors.affiliation: University of Rhode Island, Kingston, RI, USA.
authors.forename: N H
authors.initials: NH
authors.lastname: Zawia
authors.forename: Md Riyaz
authors.initials: MR
authors.lastname: Basha
authors.forename: M
authors.initials: M
authors.lastname: Razmiafshari
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008670:Metals / Q000378:metabolism*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D008670:Metals; D011506:Proteins; D015262:Xenobiotics