title: Progress and prospects of pyrrole-imidazole polyamide-fluorophore conjugates as sequence-selective DNA probes.
abstract: Recently, the versatility of N-methylpyrrole (Py)-N-methylimidazole (Im) polyamide conjugates, which have been developed from the DNA-binding antibiotics distamycin A and netropsin, has been shown. These synthetic small molecules can permeate cells to bind with duplex DNA in a sequence-specific manner, and hence can influence gene expression in vivo. Accordingly, several reports demonstrating the sequence specificity and biological activity of Py-Im polyamides have accumulated. However, the benefits of Py-Im polyamides, in particular those conjugated with fluorophores, has been overlooked. Moreover, clear directions for the employment of these attractive artificial small molecules have not yet been shown. Here, we present a detailed overview of the current and prospective applications of Py-Im polyamide-fluorophore conjugates, including sequence-specific recognition with fluorescence emission properties, and their potential roles in biological imaging.
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa oiwakecho, Kyoto 606-8502, Japan.
authors.forename: Thangavel
authors.initials: T
authors.lastname: Vaijayanthi
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Bando
authors.forename: Ganesh N
authors.initials: GN
authors.lastname: Pandian
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis*; D015342:DNA Probes / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D008958:Models, Molecular; D009757:Nylons / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes; D007093:Imidazoles; D009757:Nylons; D011758:Pyrroles; D004247:DNA