title: Analysis of glutathione-related enzymes.
abstract: Glutathione peroxidase activity can be measured using a variety of substrates to define total glutathione peroxidase activity or the activity of subsets of the family of enzymes. The assays for these activities are spectrophotometric assays, and the specificity depends on the choice of substrate and/or prior treatment of the samples by chromatography or immunoprecipitation. This unit provides the assays and support protocols for synthesizing substrates and calculating specific activity.
authors.affiliation: City of Hope National Medical Center, Duarte, California, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Esworthy
authors.forename: F F
authors.initials: FF
authors.lastname: Chu
authors.forename: J H
authors.initials: JH
authors.lastname: Doroshow
mesh_terms: D000818:Animals; D057075:Enzyme Assays / Q000379:methods*; D005978:Glutathione / Q000032:analysis*; D005979:Glutathione Peroxidase / Q000032:analysis*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D013379:Substrate Specificity
chemical_list: D006861:Hydrogen Peroxide; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase; D005978:Glutathione