title: Measurement of cytochrome P-450.
abstract: The family of cytochrome P-450 enzymes are involved in the metabolism of xenobiotics. This unit provides protocols for the three most common ways to measure cytochrome P-450. Spectrophotometric detection of the Soret band gives a quantitative measure of total cytochrome P-450 in microsomes, liver homogenate, or mitochondria, but lacks information about the specific P-450 forms. Immunochemical methods such as immunoblotting provide very sensitive semiquantitative identification of specific P-450 enzymes Finally, enzymatic assays depend on substrate specificity and also provide information about specific P-450 enzymes, although some members of this group of enzymes have broad and sometimes overlapping specificities.
authors.affiliation: University of Connecticut Health Center, Farmington, CT, USA.
authors.forename: John B
authors.initials: JB
authors.lastname: Schenkman
authors.forename: Ingela
authors.initials: I
authors.lastname: Jansson
mesh_terms: D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System