title: Change in surface properties of zirconia and initial attachment of osteoblastlike cells with hydrophilic treatment.
abstract: The objectives of this study were to characterize change in surface properties of tetragonal zirconia polycrystals (TZP) after hydrophilic treatment, and to determine the effect of such changes on initial attachment of osteoblast-like cells. Roughened surfaces were produced by alumina-blasting and acid-etching. Hydrophilic treatment comprised application of immediately after blasting and acid-etching (Blast/Etch), oxygen plasma (O2-Plasma), ultraviolet light (UV). Specimens stored in air were used as a control. The water contact angle was determined and surface analysis was performed using an X-ray photoelectron spectroscopy. Blast/Etch, O2-Plasma and UV specimens showed superhydrophilicity, and these hydrophilic treatments to TZP elicited a marked decrease in carbon content and an increase in hydroxyl groups. Hydrophilic treatments enhanced initial attachment of osteoblast-like cells and a change in cell morphologies. These results indicate that Blast/Etch, O2-Plasma, or UV treatment has potential in the creation and maintenance of superhydrophilic surfaces and enhancing initial attachment of osteoblast-like cells.
authors.affiliation: Division of Oral Implants Research, Oral Health Science Center, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Watanabe
authors.forename: Kensuke
authors.initials: K
authors.lastname: Saito
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Kokubun
authors.forename: Hodaka
authors.initials: H
authors.lastname: Sasaki
authors.forename: Masao
authors.initials: M
authors.lastname: Yoshinari
mesh_terms: D016475:3T3 Cells; D000134:Acid Etching, Dental / Q000379:methods; D000388:Air; D000537:Aluminum Oxide / Q000737:chemistry; D000818:Animals; D002244:Carbon / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D048430:Cell Shape; D045662:Dental Etching / Q000379:methods; D006858:Hydrofluoric Acid / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D006878:Hydroxides / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry; D010006:Osteoblasts / Q000502:physiology*; D010100:Oxygen / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases / Q000737:chemistry; D013499:Surface Properties; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry; D017155:Wettability; D015019:Yttrium / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*
chemical_list: D006878:Hydroxides; D058626:Plasma Gases; C499362:yttria stabilized tetragonal zirconia; D014867:Water; D015019:Yttrium; D002244:Carbon; C031356:hydroxide ion; D015040:Zirconium; D000537:Aluminum Oxide; D006858:Hydrofluoric Acid; D010100:Oxygen