title: Analysis of rutin in the extract and gel of Viola tricolor.
abstract: Heartsease, also known as wild pansy (Viola tricolor L.), contains considerable amounts of polyphenols: 109.32 ± 1.29 mg of Gallic acid equivalent/g of extract. This study investigates their phytoconstituents and antioxidant capacity and validates a method for the quantification of rutin in the crude extract of the flowers of V. tricolor and in the extract incorporated in gel. Much better antioxidant capacity was found for the extract [inhibition concentration (IC50) of 16.00 ± 0.78 µg/mL] than the standard ascorbic acid (IC50 of 16.57 ± 0.95 µg/mL); these excellent results may be attributable to the amounts of polyphenols, flavonoids and condensed tannins. The high-performance liquid chromatography method for the quantification of rutin in the extract and gel was linear, sensitive, precise, specific, accurate and robust. This validated method can be used to control the quality of the extract and the gel.
authors.affiliation: Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Piana
authors.forename: Marina
authors.initials: M
authors.lastname: Zadra
authors.forename: Thiele Faccim
authors.initials: TF
authors.lastname: de Brum
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.forename: Adiene Fernandes Kieling
authors.initials: AF
authors.lastname: Gonçalves
authors.forename: Ritiel Corrêa
authors.initials: RC
authors.lastname: da Cruz
authors.forename: Robson Borba
authors.initials: RB
authors.lastname: de Freitas
authors.forename: Gizele Scotti
authors.initials: GS
authors.lastname: do Canto
authors.forename: Margareth Linde
authors.initials: ML
authors.lastname: Athayde
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005782:Gels / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D012431:Rutin / Q000032:analysis* / Q000302:isolation & purification; D032413:Viola / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005782:Gels; D010936:Plant Extracts; D012431:Rutin