title: Genotypic diversity of clinical Coxiella burnetii isolates from Portugal based on MST and MLVA typing.
abstract: The temporal and spatial diversity of Coxiella burnetii genotypes associated with human and animal disease in Portugal was analysed using a 6-locus multiple-locus variable-number tandem repeat analysis (MLVA) and a 10-locus multi-spacer sequence typing (MST) panel. Fifteen cultured C. burnetii isolates from 13 Q fever patients and a stillborn goat and 6 additional PCR-positive ruminant tissue samples obtained during 2006-2011 were included in this study. Seven MLVA genotypes (types S-Y) were obtained, including 4 new MLVA types (U, V, W, and X), all corresponding to 3 MST profiles (types 4, 8, and 13) previously reported from France and Spain. MLVA types U-Y, all belonging to MST type 4, were found in acute Q fever patients from the districts of Évora, Faro, Lisbon, and Setúbal. Different MLVA types were associated with goats from Castelo Branco district (S) and chronic Q fever patients from both Castelo Branco and Lisboa districts (S and T), matching with MST types 13 and 8, respectively. In conclusion, a genotypic diversity of C. burnetii consistent with a non-outbreak situation was identified. The involvement of different genotypes in acute and chronic Q fever was found, linking one of the chronic genotypes to goats from the eastern region of the country.
authors.affiliation: Centre for Vectors and Infectious Diseases Research Doutor Francisco Cambournac, National Institute of Health Dr. Ricardo Jorge-CEVDI/INSA, Águas de Moura, Portugal. ana.santos@insa.min-saude.pt
authors.forename: Ana Sofia
authors.initials: AS
authors.lastname: Santos
authors.forename: Jeroen J H C
authors.initials: JJ
authors.lastname: Tilburg
authors.forename: Ana
authors.initials: A
authors.lastname: Botelho
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Barahona
authors.forename: Maria Sofia
authors.initials: MS
authors.lastname: Núncio
authors.forename: Marrigje H
authors.initials: MH
authors.lastname: Nabuurs-Franssen
authors.forename: Corné H W
authors.initials: CH
authors.lastname: Klaassen
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods; D016997:Coxiella burnetii / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D060005:Genotyping Techniques; D006041:Goats / Q000382:microbiology; D006801:Humans; D018598:Minisatellite Repeats; D058885:Multilocus Sequence Typing / Q000379:methods*; D011174:Portugal; D011778:Q Fever / Q000097:blood / Q000382:microbiology; D050497:Stillbirth / Q000662:veterinary
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer