title: Genetic deletion of HRP2 and HRP3 in Indian Plasmodium falciparum population and false negative malaria rapid diagnostic test.
abstract: Genetic polymorphisms in diagnostic antigens are important factors responsible for variable performance of rapid diagnostic tests. Additionally, the failure of antigen expression due to gene deletion may also contribute to variable performance. We report Indian Plasmodium falciparum field isolates lacking both Pfhrp2 and Pfhrp3 genes leading to false negative results of rapid diagnostic tests. The study highlights need to determine the prevalence of P. falciparum isolates lacking these genes in larger field populations in India.
authors.affiliation: National Institute of Malaria Research (ICMR), Sector 8, Dwarka, New Delhi, India. navinjh@gmail.com
authors.forename: Navin
authors.initials: N
authors.lastname: Kumar
authors.forename: Veena
authors.initials: V
authors.lastname: Pande
authors.forename: R M
authors.initials: RM
authors.lastname: Bhatt
authors.forename: Naman K
authors.initials: NK
authors.lastname: Shah
authors.forename: Neelima
authors.initials: N
authors.lastname: Mishra
authors.forename: Bina
authors.initials: B
authors.lastname: Srivastava
authors.forename: Neena
authors.initials: N
authors.lastname: Valecha
authors.forename: Anupkumar R
authors.initials: AR
authors.lastname: Anvikar
mesh_terms: D000953:Antigens, Protozoan / Q000032:analysis* / Q000235:genetics; D003955:Diagnostic Tests, Routine; D005188:False Negative Reactions; D017353:Gene Deletion; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007194:India; D016778:Malaria, Falciparum / Q000175:diagnosis* / Q000469:parasitology; D010274:Parasitology / Q000379:methods*; D010963:Plasmodium falciparum / Q000276:immunology / Q000302:isolation & purification*; D015800:Protozoan Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: D000953:Antigens, Protozoan; C514457:HRP-2 antigen, Plasmodium falciparum; C578339:HRP3 protein, Plasmodium falciparum; D015800:Protozoan Proteins