title: An economical biorefinery process for propionic acid production from glycerol and potato juice using high cell density fermentation.
abstract: An economically sustainable process was developed for propionic acid production by fermentation of glycerol using Propionibacterium acidipropionici and potato juice, a by-product of starch processing, as a nitrogen/vitamin source. The fermentation was done as high-cell-density sequential batches with cell recycle. Propionic acid production and glycerol consumption rates were dependent on initial biomass concentration, and reached a maximum of 1.42 and 2.30 g L(-1) h(-1), respectively, from 50 g L(-1) glycerol at initial cell density of 23.7 gCDW L(-1). Halving the concentration of nitrogen/vitamin source resulted in reduction of acetic and succinic acids yields by ~39% each. At glycerol concentrations of 85 and 120 g L(-1), respectively, 43.8 and 50.8 g L(-1) propionic acid were obtained at a rate of 0.88 and 0.29 g L(-1) h(-1) and yield of 84 and 78 mol%. Succinic acid was 13 g% of propionic acid and could represent a potential co-product covering the cost of nitrogen/vitamin source.
authors.affiliation: Department of Biotechnology, Center of Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden. Tarek.Dishisha@biotek.lu.se
authors.forename: Tarek
authors.initials: T
authors.lastname: Dishisha
authors.forename: Åke
authors.initials: Å
authors.lastname: Ståhl
authors.forename: Stefan
authors.initials: S
authors.lastname: Lundmark
authors.forename: Rajni
authors.initials: R
authors.lastname: Hatti-Kaul
mesh_terms: D000433:1-Propanol / Q000378:metabolism; D019342:Acetic Acid / Q000378:metabolism; D000468:Alkalies / Q000494:pharmacology; D061249:Batch Cell Culture Techniques; D018533:Biomass; D001709:Biotechnology / Q000191:economics* / Q000379:methods*; D015169:Colony Count, Microbial; D005285:Fermentation / Q000187:drug effects; D005990:Glycerol / Q000378:metabolism*; D007700:Kinetics; D009584:Nitrogen / Q000494:pharmacology; D010936:Plant Extracts / Q000378:metabolism; D011422:Propionates / Q000191:economics / Q000378:metabolism*; D011424:Propionibacterium / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011198:Solanum tuberosum / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D000468:Alkalies; D010936:Plant Extracts; D011422:Propionates; D000433:1-Propanol; D019802:Succinic Acid; C029658:propionic acid; D009584:Nitrogen; D005990:Glycerol; D019342:Acetic Acid