title: A novel glass ionomer cement containing MgCO(3 )apatite induced the increased proliferation and differentiation of human pulp cells in vitro.
abstract: This study aimed to investigate the in vitro biological response of human dental pulp cells to glass ionomer cement (GIC, Fuji IX GP(®)) containing 2.5% magnesium carbonate apatite (MgCO(3)Ap). MgCO(3)Ap was synthesized by wet method and characterized using FT-IR, XPS, and SEM. Fuji IX GP(®) served as a control. Test and control cements were prepared by encapsulated mixing the powder with Fuji IX-liquid (P/L=3.6:1). Eluates from cements extracted by 1 mL culture medium were collected at day 1, 7 and 14, and used for WST-1 proliferation assay. For ALPase activity, cells were maintained with cements in transwells, harvested and enzyme activity was measured at day 1, 4, 7, 14, and 21. We found a higher cell proliferation and increased ALPase activity by pulp cells in the test group compared to the control. This suggests the potential of GIC containing this novel biological apatite as a restorative material for pulp-dentin regeneration.
authors.affiliation: Dental Biomaterials Science, Graduate School-Interdisciplinary Program, Chulalongkorn University, 34 Henri-Dunant Rd., Pathumwan, Bangkok, Thailand.
authors.forename: Arunee
authors.initials: A
authors.lastname: Laiteerapong
authors.forename: Yossakit
authors.initials: Y
authors.lastname: Lochaiwatana
authors.forename: Isao
authors.initials: I
authors.lastname: Hirata
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okazaki
authors.forename: Kenta
authors.initials: K
authors.lastname: Mori
authors.forename: Shinya
authors.initials: S
authors.lastname: Murakami
authors.forename: Suchit
authors.initials: S
authors.lastname: Poolthong
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D001031:Apatites / Q000737:chemistry / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004396:Coloring Agents; D017077:Culture Media, Conditioned; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D005899:Glass Ionomer Cements / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008274:Magnesium / Q000737:chemistry / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013778:Tetrazolium Salts
chemical_list: C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D001031:Apatites; D004396:Coloring Agents; D017077:Culture Media, Conditioned; C439808:Fuji IX GP Fast; D005899:Glass Ionomer Cements; D013778:Tetrazolium Salts; C005479:magnesium carbonate; C030782:carboapatite; D000469:Alkaline Phosphatase; D008274:Magnesium