title: Further replication studies of the EVE Consortium meta-analysis identifies 2 asthma risk loci in European Americans.
abstract: BACKGROUND
  Genome-wide association studies of asthma have implicated many genetic risk factors, with well-replicated associations at approximately 10 loci that account for only a small proportion of the genetic risk.
  
  
  OBJECTIVE
  We aimed to identify additional asthma risk loci by performing an extensive replication study of the results from the EVE Consortium meta-analysis.
  
  
  METHODS
  We selected 3186 single nucleotide polymorphisms for replication based on the P values from the EVE Consortium meta-analysis. These single nucleotide polymorphisms were genotyped in ethnically diverse replication samples from 9 different studies, totaling 7202 cases, 6426 controls, and 507 case-parent trios. Association analyses were conducted within each participating study, and the resulting test statistics were combined in a meta-analysis.
  
  
  RESULTS
  Two novel associations were replicated in European Americans: rs1061477 in the KLK3 gene on chromosome 19 (combined odds ratio = 1.18; 95% CI, 1.10-1.25) and rs9570077 (combined odds ratio =1.20; 95% CI, 1.12-1.29) on chromosome 13q21. We could not replicate any additional associations in the African Americans or Latinos.
  
  
  CONCLUSIONS
  This extended replication study identified 2 additional asthma risk loci in populations of European descent. The absence of additional loci for African Americans and Latinos highlights the difficulty in replicating associations in admixed populations.
authors.affiliation: Department of Human Genetics, University of Chicago, Chicago, IL, USA.
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Myers
authors.forename: Blanca E
authors.initials: BE
authors.lastname: Himes
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Gignoux
authors.forename: James J
authors.initials: JJ
authors.lastname: Yang
authors.forename: W James
authors.initials: WJ
authors.lastname: Gauderman
authors.forename: Cristina
authors.initials: C
authors.lastname: Rebordosa
authors.forename: Jianming
authors.initials: J
authors.lastname: Xie
authors.forename: Dara G
authors.initials: DG
authors.lastname: Torgerson
authors.forename: Albert M
authors.initials: AM
authors.lastname: Levin
authors.forename: James
authors.initials: J
authors.lastname: Baurley
authors.forename: Penelope E
authors.initials: PE
authors.lastname: Graves
authors.forename: Rasika A
authors.initials: RA
authors.lastname: Mathias
authors.forename: Isabelle
authors.initials: I
authors.lastname: Romieu
authors.forename: Lindsey A
authors.initials: LA
authors.lastname: Roth
authors.forename: David
authors.initials: D
authors.lastname: Conti
authors.forename: Lydiana
authors.initials: L
authors.lastname: Avila
authors.forename: Celeste
authors.initials: C
authors.lastname: Eng
authors.forename: Hita
authors.initials: H
authors.lastname: Vora
authors.forename: Michael A
authors.initials: MA
authors.lastname: LeNoir
authors.forename: Manuel
authors.initials: M
authors.lastname: Soto-Quiros
authors.forename: Jinghua
authors.initials: J
authors.lastname: Liu
authors.forename: Juan C
authors.initials: JC
authors.lastname: Celedón
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Galanter
authors.forename: Harold J
authors.initials: HJ
authors.lastname: Farber
authors.forename: Rajesh
authors.initials: R
authors.lastname: Kumar
authors.forename: Pedro C
authors.initials: PC
authors.lastname: Avila
authors.forename: Kelley
authors.initials: K
authors.lastname: Meade
authors.forename: Denise
authors.initials: D
authors.lastname: Serebrisky
authors.forename: Shannon
authors.initials: S
authors.lastname: Thyne
authors.forename: William
authors.initials: W
authors.lastname: Rodriguez-Cintron
authors.forename: Jose R
authors.initials: JR
authors.lastname: Rodriguez-Santana
authors.forename: Luisa N
authors.initials: LN
authors.lastname: Borrell
authors.forename: Robert F
authors.initials: RF
authors.lastname: Lemanske
authors.forename: Eugene R
authors.initials: ER
authors.lastname: Bleecker
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Meyers
authors.forename: Stephanie J
authors.initials: SJ
authors.lastname: London
authors.forename: Kathleen C
authors.initials: KC
authors.lastname: Barnes
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Raby
authors.forename: Fernando D
authors.initials: FD
authors.lastname: Martinez
authors.forename: Frank D
authors.initials: FD
authors.lastname: Gilliland
authors.forename: L Keoki
authors.initials: LK
authors.lastname: Williams
authors.forename: Esteban G
authors.initials: EG
authors.lastname: Burchard
authors.forename: Scott T
authors.initials: ST
authors.lastname: Weiss
authors.forename: Dan L
authors.initials: DL
authors.lastname: Nicolae
authors.forename: Carole
authors.initials: C
authors.lastname: Ober
mesh_terms: D001741:Black or African American; D001249:Asthma / Q000453:epidemiology* / Q000235:genetics* / Q000276:immunology; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D004252:DNA Mutational Analysis; D056426:Genetic Loci / Q000276:immunology; D020022:Genetic Predisposition to Disease; D055106:Genome-Wide Association Study; D006630:Hispanic or Latino; D006801:Humans; D007610:Kallikreins / Q000235:genetics*; D015201:Meta-Analysis as Topic; D020641:Polymorphism, Single Nucleotide; D017430:Prostate-Specific Antigen / Q000235:genetics*; D012307:Risk Factors; D014481:United States; D044465:White People
chemical_list: C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen