title: Expression of pancreatic endocrine markers by mesenchymal stem cells from human adipose tissue.
abstract: Mesenchymal stem cells (MSCs) from human adipose tissue have a great potential for use in cell therapy due to their ease of isolation, expansion, and differentiation, besides the relative acceptance from the ethical point of view. Our intention was to isolate and promote in vitro expansion and differentiation of MSCs from human adipose tissue into cells with a pancreatic endocrine phenotype. Human adipose tissue obtained from patients undergoing abdominal dermolipectomy was digested with type I collagenase. MSCs isolated by plastic adherence and characterized by cytochemistry and FACS were expanded in vitro. MSC differentiation into an endocrine phenotype was induced over 2 to 4 months with high glucose (25 mmol/L) media containing nicotinamide, exendin-4, and 2-mercaptoethanol. Insulin and glucagon expressions were analyzed by immunofluorescence. Cells isolated from human adipose tissue and expanded in vitro expressed MSC markers as confirmed by FACS and cytochemistry. Insulin but not glucagon production by differentiated cells was demonstrated by immunofluorescence. MSCs isolated from human adipose tissue were induced to differentiate in vitro into an endocrine phenotype that expressed insulin.
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Silva
authors.forename: L S
authors.initials: LS
authors.lastname: Percegona
authors.forename: A L
authors.initials: AL
authors.lastname: França
authors.forename: T M
authors.initials: TM
authors.lastname: Dos Santos
authors.forename: C C
authors.initials: CC
authors.lastname: Perini
authors.forename: P
authors.initials: P
authors.lastname: González
authors.forename: C L K
authors.initials: CL
authors.lastname: Rebelatto
authors.forename: N O S
authors.initials: NO
authors.lastname: Câmara
authors.forename: C A M
authors.initials: CA
authors.lastname: Aita
mesh_terms: D050153:Abdominal Fat / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000235:genetics / Q000378:metabolism*; D007515:Islets of Langerhans / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010641:Phenotype; D016133:Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D007328:Insulin; D005947:Glucose