title: Comparison of test methods to screen for residual chemical contamination on medical device surfaces.
abstract: Reprocessing medical devices involves several steps including cleaning and disinfection or sterilization. Chemical residuals can occur at various stages of reprocessing. These residues could interfere with device function and potentially harm patients. These solutions are composed of a combination of various chemicals and their residues are highly diluted post rinsing, therefore, it is difficult to find a sensitive and rapid method to detect toxicity due to chemical residues. This study focused on (1) finding the levels of residues that are cytotoxic using two mammalian cell lines and Daphnia magna, and (2) evaluating two test methods, Total Organic Carbon (TOC) and the Luminescent Bacteria Test (LBT), to measure residual chemicals levels. The two mammalian cell lines were equal in their cytotoxicity responses. However, Daphnia were more sensitive to some chemical residue than the two mammalian cell lines. TOC and LBT were able to detect the presence of residue well below the levels that were determined to cause mammalian cytotoxicity. LBT was more sensitive for some chemicals and TOC for others, both in solution and in simulated cleaning and rinsing for the limited number of solutions tested in this study.
authors.affiliation: U.S Food and Drug Administration, Center for Devices and Radiological Health, SIlver Spring, MD, USA.
authors.forename: Prachi
authors.initials: P
authors.lastname: Kulkarni
authors.forename: Megan
authors.initials: M
authors.lastname: Shoff
authors.forename: Anne
authors.initials: A
authors.lastname: Lucas
mesh_terms: D001681:Biological Assay / Q000295:instrumentation* / Q000379:methods*; D002244:Carbon / Q000032:analysis*; D004866:Equipment Contamination / Q000517:prevention & control*; D019544:Equipment Failure Analysis / Q000379:methods*; D004864:Equipment and Supplies / Q000382:microbiology*; D009930:Organic Chemicals / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties
chemical_list: D009930:Organic Chemicals; D002244:Carbon