title: Detection of cytomegalovirus and human herpesvirus-6 DNA in liver biopsy specimens and their correlation with rejection after liver transplantation.
abstract: Cytomegalovirus (CMV) and human herpesvirus-6 (HHV-6) reactivation after transplantation put patients at an increased risk of graft rejection mainly among those who receive organs that are positive in their donor biopsies. The aim of this study was to investigate the presence of CMV and HHV-6 DNA in liver biopsy specimens from the donors and from their grafts for correlation with rejection after transplantation. We followed 41 liver transplantation patients whose samples were evaluated using nested-polymerase chain reactions (N-PCR). Twenty-one (51%) of the 41 studied patients experienced rejection; 4/21 (19%) were CMV positive in the donor biopsy specimens and remained positive; another 5 subjects became positive. The patients who received organs from donors with biopsies positive for CMV demonstrated a trend to develop graft rejection after transplantation (Fisher's exact test [P = .0591] with significant results on univariate and multivariate analysis [P = .042]). Eight of the 21 who experienced rejection episodes were HHV-6 positive in the donor biopsy but there was no statistical significance CMV DNA diagnosed in liver donor biopsies remained positive posttransplantation in liver biopsy recipients; it was considered a tendency to develop acute cellular rejection after transplantation.
authors.affiliation: Unit of Liver Transplantation, State University of Campinas, SP, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Guardia-Silva
authors.forename: R S B
authors.initials: RS
authors.lastname: Stucchi
authors.forename: A M
authors.initials: AM
authors.lastname: Sampaio
authors.forename: A
authors.initials: A
authors.lastname: Milan
authors.forename: S C B
authors.initials: SC
authors.lastname: Costa
authors.forename: I F S F
authors.initials: IF
authors.lastname: Boin
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D001706:Biopsy; D016009:Chi-Square Distribution; D003587:Cytomegalovirus / Q000235:genetics*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006084:Graft Rejection / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D015654:Herpesvirus 6, Human / Q000235:genetics*; D006801:Humans; D016031:Liver Transplantation / Q000009:adverse effects* / Q000276:immunology; D016015:Logistic Models; D008137:Longitudinal Studies; D008297:Male; D015999:Multivariate Analysis; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011446:Prospective Studies; D018570:Risk Assessment; D012307:Risk Factors; D019349:Roseolovirus Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D014019:Tissue Donors; D016896:Treatment Outcome; D014775:Virus Activation; D055815:Young Adult
chemical_list: D004279:DNA, Viral