title: Human elastin-based recombinant biopolymers improve mesenchymal stem cell differentiation.
abstract: Elastin-based polypeptides are a class of smart biopolymers representing an important model in the design of biomaterials. The combination of biomimetic materials with cells that have great plasticity provides a promising strategy for the realization of highly engineered cell-based constructs for regenerative medicine and tissue repair applications. Two recombinant biopolymers inspired by human elastin are assessed as coating agents to prepare biomimetic surfaces for cell culture. These substrates are assayed for hBM MSC culture. The coated surfaces are also characterized with AFM to evaluate the topographical features of the deposited biopolymers. The results suggest that the elastin-derived biomimetic surfaces play a stimulatory role on osteogenic differentiation of MSCs.
authors.affiliation: Laboratory for Biomaterials and Bioengineering, Laval University, Quebec City, G1V 0A6, PQ, Canada.
authors.forename: Betül
authors.initials: B
authors.lastname: Çelebi
authors.forename: Maxime
authors.initials: M
authors.lastname: Cloutier
authors.forename: Rodrigo B
authors.initials: RB
authors.lastname: Rabelo
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Balloni
authors.forename: Diego
authors.initials: D
authors.lastname: Mantovani
authors.forename: Antonella
authors.initials: A
authors.lastname: Bandiera
mesh_terms: D000595:Amino Acid Sequence; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology*; D001704:Biopolymers / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology*; D004549:Elastin / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D010011:Osteocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D023822:Tissue Engineering
chemical_list: D001704:Biopolymers; D020099:Coated Materials, Biocompatible; D011994:Recombinant Proteins; D004549:Elastin