title: Detection of heme oxygenase activity by measurement of CO.
abstract: Heme oxygenase (HO) is the first and rate-limiting step in degradation of heme, and in the presence of NADPH-cytochrome P-450 reductase it produces equimolar amounts of biliverdin and CO. CO produced in a closed system can be quantified as described in this unit by gas chromatography as a measure of HO activity in tissue slices, tissue homogenates, and tissue fractions.
authors.affiliation: Stanford University Medical Center, Stanford, California, USA.
authors.forename: H J
authors.initials: HJ
authors.lastname: Vreman
authors.forename: D K
authors.initials: DK
authors.lastname: Stevenson
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D002248:Carbon Monoxide / Q000302:isolation & purification* / Q000378:metabolism; D002849:Chromatography, Gas; D004867:Equipment Design; D006418:Heme / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results
chemical_list: D006418:Heme; D002248:Carbon Monoxide; D006419:Heme Oxygenase (Decyclizing)