title: In vitro generation of platelets through direct conversion: first report in My Knowledge (iMK).
abstract: Blood transfusion medicine requires a constant supply of platelets, which is now totally donor dependent. Recent advances in the generation of platelets in vitro through megakaryocytes (MKs) may provide protocols not only via pluripotent stem cells, including induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), but also via induced MKs (iMKs). For the first time, mouse and human fibroblasts are successfully transdifferentiated into iMKs by the introduction of three factors, p45NF-E2, Maf G, and Maf K.
authors.affiliation: Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Masuda
authors.forename: Mo
authors.initials: M
authors.lastname: Li
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Izpisua Belmonte
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001792:Blood Platelets / Q000166:cytology; D002460:Cell Line; D054338:Cell Transdifferentiation; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D050984:NF-E2 Transcription Factor, p45 Subunit / Q000235:genetics / Q000378:metabolism; D051257:Proto-Oncogene Proteins c-maf / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism
chemical_list: D050984:NF-E2 Transcription Factor, p45 Subunit; D051257:Proto-Oncogene Proteins c-maf; D016271:Proto-Oncogene Proteins c-myc