title: LC-MS N-alkylamide profiling of an ethanolic Anacyclus pyrethrum root extract.
abstract: An N-alkylamide profiling from an ethanolic Anacyclus pyrethrum DC. root extract was performed using a gradient reversed phase high performance liquid chromatography/UV/electrospray-ionization ion-trap mass spectrometry (HPLC/UV/ESI-MS) method on an embedded polar column. MS1 and MS2 fragmentation data were used for identification purposes while UV was used for quantification. Thirteen N-alkylamides (five N-isobutylamides, three N-methyl isobutylamides, four tyramides and one 2-phenylethylamide) were detected. Six of them, identified as undeca-2E,4E-diene-8,10-diynoic acid isobutylamide, undeca-2E,4E-diene-8,10-diynoic acid N-methyl isobutylamide, tetradeca-2E,4E-diene-8,10-diynoic acid tyramide, deca-2E,4E-dienoic acid N-methyl isobutylamide, tetradeca-2E,4E,XE/Z-trienoic acid tyramide and tetradeca-2E,4E,XE/Z,YE/Z-tetraenoic isobutylamide, are novel compounds which have never been reported before from Anacyclus pyrethrum.
authors.affiliation: Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
authors.forename: Jente
authors.initials: J
authors.lastname: Boonen
authors.forename: Vikas
authors.initials: V
authors.lastname: Sharma
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Dixit
authors.forename: Christian
authors.initials: C
authors.lastname: Burvenich
authors.forename: Bart
authors.initials: B
authors.lastname: De Spiegeleer
mesh_terms: D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000302:isolation & purification; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D053284:Polyunsaturated Alkamides / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: C575818:2,4-undeca-2,4-diene-8,10-diynoic acid isobutylamide; D005231:Fatty Acids, Unsaturated; D010936:Plant Extracts; D053284:Polyunsaturated Alkamides; D000431:Ethanol; C008778:pellitorine