title: Determination of estrogens in pork and chicken samples by stir bar sorptive extraction combined with high-performance liquid chromatography-ultraviolet detection.
abstract: A poly(dimethylsiloxane) (PDMS)/β-cyclodextrin (β-CD)/divinylbenzene (DVB)-coated stir bar was prepared by the sol-gel technique for the stir bar sorptive extraction (SBSE) of four estrogens from animal-derived foods, followed by liquid desorption (LD) and high-performance liquid chromatography-ultraviolet (HPLC-UV) detection. The influence of the coating composition on SBSE of target estrogens was investigated by an orthogonal experiment design, and the prepared PDMS/β-CD/DVB-coated stir bars show good reproducibility. Under the optimal experimental conditions, the limits of detection (S/N = 3) of the developed PDMS/β-CD/DVB SBSE-LD-HPLC-UV method were 0.21-1.6 μg/L for the target estrogens with enrichment factors of 19-51-fold, the dynamic linear range was 2-2000 μg/L, and the relative standard deviations of the method ranged from 6.0% to 9.7% (n = 8, c = 100 μg/L) and from 8.4% to 11.7% (n = 8, c = 10 μg/L). The developed method was simple, sensitive, and selective and was successfully applied to the analysis of estrogens in pork and chicken samples.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, People's Republic of China.
authors.forename: Cong
authors.initials: C
authors.lastname: Hu
authors.forename: Man
authors.initials: M
authors.lastname: He
authors.forename: Beibei
authors.initials: B
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Hu
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004967:Estrogens / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D011202:Poultry Products / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013552:Swine
chemical_list: D004967:Estrogens