title: Angiopoietin 1 enhances the proliferation and differentiation of skeletal myoblasts.
abstract: Angiopoietin 1 (Ang1) plays an important role in various endothelial functions, such as vascular integrity and angiogenesis; however, less is known about its function outside of the endothelium. In this study, we examined whether Ang1 has direct effects on skeletal muscle cells. We found that Ang1 exhibited myogenic potential, as it promoted the proliferation, migration, and differentiation of mouse primary skeletal myoblasts. The positive effect of Ang1 on myoblast proliferation could have been mediated by the α7 and β1 integrins. We also found that Ang1 potentiated cellular Ca(2+) movements in differentiated myotubes in response to stimuli, possibly through the increased expression of two Ca(2+) -related proteins, namely, Orai1 and calmodulin. Ang1 also increased Orai1 and calmodulin expression in mouse hearts in vivo. These results provide an insight into the molecular mechanisms by which Ang1 directly affects the myogenesis of striated muscle.
authors.affiliation: Department of Physiology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Eun Hui
authors.initials: EH
authors.lastname: Lee
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Woo
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Hwang
authors.forename: Jae-Hyeong
authors.initials: JH
authors.lastname: Park
authors.forename: Chung-Hyun
authors.initials: CH
authors.lastname: Cho
mesh_terms: D042683:Angiopoietin-1 / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D039001:Integrin alpha Chains / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D051379:Mice; D024510:Muscle Development; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000378:metabolism; D018919:Neovascularization, Physiologic; D000071740:ORAI1 Protein
chemical_list: D042683:Angiopoietin-1; D015703:Antigens, CD; D015220:Calcium Channels; D002147:Calmodulin; D039001:Integrin alpha Chains; D019012:Integrin beta1; D000071740:ORAI1 Protein; C520430:Orai1 protein, mouse; C085725:integrin alpha7; D002118:Calcium