title: The anti-inflammatory effects of ethyl acetate on Lipopolysaccharide/D-galactosamine challenged mice and Lipopolysaccharide activated RAW264.7 cells.
abstract: Ethyl acetate (EA) is an ordinary organic compound in fruits, wine and cosmetics, and used as a solvent frequently. With the recent observation in our experiment, we suspected that EA could affect immune function, in particular macrophage activity. In this paper, we tested EA's protect effect against death in Lipopolysaccharide/D-galactosamine (LPS/D-GalN)-induced endotoxic shock model in mice. And also found EA decreased the LPS-induced mRNA expression of mediators of inflammation including cyclooxygenase 2 (COX2), inducible NOS (iNOS), and tumor necrosis factor α (TNF α) in RAW264.7 cells. Consequently, EA decreased the production of, TNF α and the inflammatory agent nitric oxide (NO) in RAW264.7 cells treated with LPS. Other pro-inflammatory cytokines such as IL-1h and IL-6 were similarly decreased by EA treatment of RAW264.7 cells. The potential mechanism may associate with NF-κB activity as we shown. Taken together, these results suggest that EA has anti-inflammatory properties.
authors.affiliation: Laboratory of Cell Pharmacology, College of Pharmaceutical Sciences, Hebei University, 180 East Wusi Road, Baoding 071002, China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Liu
authors.forename: Nan
authors.initials: N
authors.lastname: Xiao
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Liu
authors.forename: Belinda
authors.initials: B
authors.lastname: Wilson
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Cao
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000085:Acetates / Q000627:therapeutic use*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D002460:Cell Line; D004195:Disease Models, Animal; D005688:Galactosamine / Q000276:immunology; D015870:Gene Expression / Q000187:drug effects; D053583:Interleukin-1beta / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D021381:Protein Transport; D012772:Shock, Septic / Q000188:drug therapy* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000085:Acetates; D000894:Anti-Inflammatory Agents, Non-Steroidal; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D005688:Galactosamine; C007650:ethyl acetate; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse