title: Induction of antifouling diterpene production by Streptomyces cinnabarinus PK209 in co-culture with marine-derived Alteromonas sp. KNS-16.
abstract: An active antifouling diterpene was isolated from marine actinomycete strain PK209 and productivity was induced in a co-culture experiment. The active constituent was identified as the diterpene lobocompactol by interpretion of nuclear magnetic resonance and mass spectroscopy data. A PK209 co-culture was designed and a lobocompactol-resistant bacterium, KNS-16, was selected as co-culture competitor to induce lobocompactol production. Adding a small volume of 16-h-old KNS-16 culture to the 96-h-old PK209 culture caused rapid induction of lobocompactol production. The final yield was 2.7 mg/L, 10.4-fold higher than that collected from a single PK209 culture. The two bacteria, strains PK209 and KNS-16, were identified as Streptomyces cinnabarinus and Alteromonas sp. based on 16S rDNA sequencing. Lobocompactol showed significant antifouling activity, of 0.18 and 0.43 µg/mL, for EC₅₀ against the macroalga Ulva pertusa and the diatom Navicula annexa respectively. It showed activity with MIC of 61-112 µg/mL against fouling bacteria.
authors.affiliation: Department of Marine Biotechnology, SoonChunHyang University, Asan, Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Cho
authors.forename: Myoung Sug
authors.initials: MS
authors.lastname: Kim
mesh_terms: D020591:Alteromonas / Q000254:growth & development* / Q000378:metabolism; D059001:Aquatic Organisms / Q000254:growth & development* / Q000378:metabolism; D057219:Biofouling / Q000517:prevention & control*; D018920:Coculture Techniques; D004224:Diterpenes / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D013302:Streptomyces / Q000254:growth & development* / Q000378:metabolism*; D044445:Ulva / Q000187:drug effects
chemical_list: D004224:Diterpenes; C579333:lobocompactol