title: Cochlin expression in the rat perilymph during postnatal development.
abstract: CONCLUSIONS
  The changes in the cochlin isoforms in the perilymph may provide important insights to the understanding of cochlin function and the pathogenesis of related inner ear diseases.
  
  
  OBJECTIVE
  Cochlin is involved in various pathologies of the inner ear. Altered levels of cochlin isoforms in developing inner ear tissue were reported previously. The purpose of this study was to elucidate the cochlin isoform expression in the perilymph of rats during postnatal development in relation to Coch gene mRNA expression.
  
  
  METHODS
  We studied the cochlin isoforms in the rat perilymph during postnatal development by Western blot analysis. Real-time PCR was also performed to elucidate the expression level of Coch mRNA in the developing inner ear of rats.
  
  
  RESULTS
  Western blot analysis showed that the expression of p63s in the perilymph was highest on the 12th day after birth (DAB12), the earliest age at which we could identify the perilymphatic space microscopically, and it decreased gradually as the cochlea developed. On the other hand, the expression of Cochlin-tomoprotein (CTP)was lowest on DAB12 and increased gradually up to DAB24. COCH mRNA was detected from DAB3 and gradually increased to DAB15, and then gradually decreased to DAB70.
authors.affiliation: Department of Otorhinolaryngology, Nippon Medical School, Tokyo, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Shiiba
authors.forename: Susumu
authors.initials: S
authors.lastname: Shindo
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Ikezono
authors.forename: Kuwon
authors.initials: K
authors.lastname: Sekine
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Matsumura
authors.forename: Satomi
authors.initials: S
authors.lastname: Sekiguchi
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Yagi
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Okubo
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004423:Ear / Q000254:growth & development*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D010498:Perilymph / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C529734:Coch protein, rat; D016326:Extracellular Matrix Proteins; D020033:Protein Isoforms