title: Estrogen receptor alpha localization in the testes of men with normal spermatogenesis.
abstract: It is known that estrogens act on the male reproductive tract by binding to estrogen receptors (ER) α and β. However, studies on ER localization in the human testis are discordant. The aim of this study was to investigate the localization of ERα in the testes of adult men with normal spermatogenesis. Semen analysis of ten adult men revealed azoospermia. FSH, LH and testosterone serum concentrations were within normal values, and the volume of the testes was normal, hence obstructive azoospermia was suspected. The tissues from testicular surgical biopsies were fixed in Bouin's fluid and embedded in paraffin. Assessments of the seminiferous epithelium (scoring 10 to -1), the number of Leydig cells (scoring 1 to 5), the areal fraction of intertubular space (IS), measurements of seminiferous tubule diameter, and the thickness of the tubular wall, were performed on microscopic sections. Immunohistochemical staining was applied with monoclonal antibodies against ERα. The mean spermatogenesis score was 10 points; IS - 30.6 ± 8.1%; seminiferous tubule diameter - 193.9 ± 19.4 μm; thickness of tubular wall - 7.44 ± 1.1 μm; number of Leydig cells - 1.6 ± 1.1 points. Immunohistochemical staining showed the localization of ERα to be in the Sertoli and Leydig cell cytoplasm, while ERα was absent in germ cells. The results of testicular tissue analysis confirmed its normal structure and normal, full spermatogenesis. The presence of ERα in Sertoli and Leydig cells in normal human testis demonstrated in this study suggests that estrogens may affect testicular function.
authors.affiliation: Department of Reproductive Endocrinology, Medical University of Lodz, Poland.
authors.forename: Eliza
authors.initials: E
authors.lastname: Filipiak
authors.forename: Dagmara
authors.initials: D
authors.lastname: Suliborska
authors.forename: Maria
authors.initials: M
authors.lastname: Laszczynska
authors.forename: Renata
authors.initials: R
authors.lastname: Walczak-Jedrzejowska
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Oszukowska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Marchlewska
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kula
authors.forename: Jolanta
authors.initials: J
authors.lastname: Slowikowska-Hilczer
mesh_terms: D000328:Adult; D047628:Estrogen Receptor alpha / Q000378:metabolism*; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D009929:Organ Size; D021381:Protein Transport; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology / Q000378:metabolism*; D013739:Testosterone / Q000097:blood
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone