title: High production of cellulose degrading endo-1,4-β-D-glucanase using bagasse as a substrate from Bacillus subtilis KIBGE HAS.
abstract: Sugarcane bagasse is a cheap carbon source for endo-1,4-β-D-glucanase production as it is easily available as by-product from sugar industries. Fermentation conditions for endo-1,4-β-D-glucanase production by Bacillus subtilis KIBGE HAS were optimized by using un-treated sugarcane bagasse for induction of endo-1,4-β-D-glucanase and it was found that 2.0 g% bagasse in fermentation medium induced maximum endo-1,4-β-D-glucanase production. It was also found that when sugarcane bagasse was supplemented with different carbon sources, the results showed that lactose, xylose, maltose and sucrose favored endo-1,4-β-D-glucanase production, whereas cellobiose and fructose inhibit enzyme production. Maximum endo-1,4-β-D-glucanase production was obtained at 40 °C keeping the initial pH of the medium at 7.0 before sterilization. Maximum endo-1,4-β-D-glucanase production was obtained after 48 h incubation. Among different nitrogen sources, ammonium nitrate enhanced endo-1,4-β-D-glucanase production. The optimal temperature and pH for enzyme activity were 60 °C and 7.0, respectively.
authors.affiliation: Pharmaceutical Research Centre, PCSIR Laboratories complex, Karachi, Pakistan.
authors.forename: Saeeda
authors.initials: S
authors.lastname: Bano
authors.forename: Shah Ali Ul
authors.initials: SA
authors.lastname: Qader
authors.forename: Afsheen
authors.initials: A
authors.lastname: Aman
authors.forename: Mohammad Noman
authors.initials: MN
authors.lastname: Syed
authors.forename: Kamran
authors.initials: K
authors.lastname: Durrani
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D002480:Cellulase / Q000096:biosynthesis* / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000378:metabolism; D031786:Saccharum / Q000737:chemistry; D013696:Temperature
chemical_list: D002244:Carbon; D002482:Cellulose; C027433:bagasse; D002480:Cellulase; D009584:Nitrogen