title: Laboratory methods to study African swine fever virus.
abstract: We summarize findings of comparative studies in different cells cultures susceptible to ASFV infection, through the analysis of virus components and infectious virus particles production, as alternative means to grow field and laboratory ASFV strains. We also provide different methods to assay the infectivity of ASFV samples and to purify the infective virus particles. Finally we describe the general strategy to construct virus deletion mutants that can be engineered to obtain attenuated ASFV strains suitable for vaccine approaches.
authors.affiliation: Centro de Biologia Molecular Severo Ochoa (CSIC-UAM), Universidad Autonoma de Madrid, c/Nicolas Cabrera no 1, 28049 Madrid, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: de León
authors.forename: María J
authors.initials: MJ
authors.lastname: Bustos
authors.forename: Angel L
authors.initials: AL
authors.lastname: Carrascosa
mesh_terms: D000358:African Swine Fever Virus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D017353:Gene Deletion; D008967:Molecular Biology / Q000379:methods*; D014773:Virology / Q000379:methods*