title: Role of physiological levels of 4-hydroxynonenal on adipocyte biology: implications for obesity and metabolic syndrome.
abstract: Lipid peroxidation products such as 4-hydroxynonenal (HNE) are known to be increased in response to oxidative stress, and are known to cause dysfunction and pathology in a variety of tissues during periods of oxidative stress. The aim of the current study was to determine the chronic (repeated HNE exposure) and acute effects of physiological concentrations of HNE toward multiple aspects of adipocyte biology using differentiated 3T3-L1 adipocytes. Our studies demonstrate that acute and repeated exposure of adipocytes to physiological concentrations of HNE is sufficient to promote subsequent oxidative stress, impaired adipogenesis, alter the expression of adipokines, and increase lipolytic gene expression and subsequent increase in free fatty acid (FFA) release. These results provide an insight in to the role of HNE-induced oxidative stress in regulation of adipocyte differentiation and adipose dysfunction. Taken together, these data indicate a potential role for HNE promoting diverse effects toward adipocyte homeostasis and adipocyte differentiation, which may be important to the pathogenesis observed in obesity and metabolic syndrome.
authors.affiliation: Pennington Biomedical Research Center , Louisiana State University System, Baton Rouge, LA 70808, USA.
authors.forename: Kalavathi
authors.initials: K
authors.lastname: Dasuri
authors.forename: Philip
authors.initials: P
authors.lastname: Ebenezer
authors.forename: Sun Ok
authors.initials: SO
authors.lastname: Fernandez-Kim
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhanguo
authors.initials: Z
authors.lastname: Gao
authors.forename: Annadora J
authors.initials: AJ
authors.lastname: Bruce-Keller
authors.forename: Linnea R
authors.initials: LR
authors.lastname: Freeman
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Keller
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D050156:Adipogenesis; D054392:Adipokines / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000502:physiology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D024821:Metabolic Syndrome / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D054392:Adipokines; D000447:Aldehydes; D005230:Fatty Acids, Nonesterified; D017382:Reactive Oxygen Species; C027576:4-hydroxy-2-nonenal