title: Preparation of small RNA libraries for high-throughput sequencing.
abstract: This protocol details the process of small RNA cloning for sequencing on the Illumina/Solexa sequencing platform, but it can be easily modified for use on other next-generation platforms (e.g., SOLiD, 454). This procedure is designed to clone canonical small RNA molecules with 5'-monophosphate and 3'-hydroxyl termini. Modifications, such as the presence of a 2'-O-methyl group, can reduce efficiency, although not sufficiently to negate the utility of the approach. Other termini modifications, such as a 5' triphosphate or a 3' phosphate, can be altered by enzymatic treatment before cloning.
authors.forename: Colin
authors.initials: C
authors.lastname: Malone
authors.forename: Julius
authors.initials: J
authors.lastname: Brennecke
authors.forename: Ben
authors.initials: B
authors.lastname: Czech
authors.forename: Alexei
authors.initials: A
authors.lastname: Aravin
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Hannon
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012313:RNA