title: Production and characterization of carboxymethyl cellulase from Paenibacillus polymyxa using mango peel as substrate.
abstract: Mango peel, a solid mango processing waste, comprises 15-20% of total fruit weight. This, being a rich source of lignocelluloses, was used as substrate for carboxymethyl cellulase (CMCase) production using Paenibacillus polymyxa. Maximum CMCase production (7.814 U mg(-1)) was observed in a medium containing 7% mango peel (w/v) with 1.5% ammonium sulphate (w/v) at 37 degrees C and pH 5.5. Purification to an extent of 28.24 fold was achieved by affinity column chromatography. Bands corresponding to 26.5 and 34.0 kDa molecular sizes were observed on 12% denaturing Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) while of 72 kDa on 10% non-denaturing Native-PAGE, proving its heteromeric multienzyme nature. The enzyme was stable over a range of 20-60 degrees C and pH of 4.0-7.5. Michaelis-Menten equation constant (Km and Vmax) values of purified CMCase were 8.73 mg ml(-1) and 17.805 mM ml(-1) min(-1), respectively.
authors.affiliation: Central Institute for Subtropical Horticulture, Rehmankhera, Lucknow - 227 107, India.
authors.forename: Devendra
authors.initials: D
authors.lastname: Kumar
authors.forename: Mohd
authors.initials: M
authors.lastname: Ashfaque
authors.forename: M
authors.initials: M
authors.lastname: Muthukumar
authors.forename: Munna
authors.initials: M
authors.lastname: Singh
authors.forename: Neelima
authors.initials: N
authors.lastname: Garg
mesh_terms: D002480:Cellulase / Q000096:biosynthesis* / Q000378:metabolism; D005591:Chemical Fractionation; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D031022:Mangifera; D056507:Paenibacillus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods
chemical_list: D002480:Cellulase; C019826:carboxymethylcellulase