title: MicroRNA-466l inhibits antiviral innate immune response by targeting interferon-alpha.
abstract: Effective recognition of viral infections and subsequent triggering of antiviral innate immune responses are essential for the host antiviral defense, which is tightly regulated by multiple regulators, including microRNAs (miRNAs). A previous study showed that miR-466l upregulates IL-10 expression in macrophages by antagonizing RNA-binding protein tristetraprolin-mediated IL-10 mRNA degradation. However, the ability of miR-466l to regulate antiviral immune responses remains unknown. Here, we found that interferon-alpha (IFN-α) expression was repressed in Sendai virus (SeV)- and vesicular stomatitis virus (VSV)-infected macrophages and in dendritic cells transfected with miR-466l expression. Moreover, multiple IFN-α species can be directly targeted by miR-466l through their 3' untranslated region (3'UTR). This study has demonstrated that miR-466l could directly target IFN-α expression to inhibit host antiviral innate immune response.
authors.affiliation: Department of Anesthesiology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
authors.forename: Yingke
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Fan
authors.forename: Xingying
authors.initials: X
authors.lastname: He
authors.forename: Haijing
authors.initials: H
authors.lastname: Sun
authors.forename: Zui
authors.initials: Z
authors.lastname: Zou
authors.forename: Hongbin
authors.initials: H
authors.lastname: Yuan
authors.forename: Haitao
authors.initials: H
authors.lastname: Xu
authors.forename: Chengcai
authors.initials: C
authors.lastname: Wang
authors.forename: Xueyin
authors.initials: X
authors.lastname: Shi
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology*; D016898:Interferon-alpha / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D029082:Sendai virus / Q000276:immunology*; D014158:Transcription, Genetic; D018116:Vesiculovirus / Q000276:immunology*; D014779:Virus Replication / Q000276:immunology
chemical_list: D020413:3' Untranslated Regions; D016898:Interferon-alpha; D035683:MicroRNAs; C576133:Mirn466 microRNA, mouse; D012333:RNA, Messenger