title: Enhancement of antigen presenting ability in the leukemic plasmacytoid dendritic cell line (PMDC05) by lentiviral vector-mediated transduction of CD80 gene.
abstract: PMDC05, a leukemic plasmacytoid dendritic cell (pDC) line which was established in our laboratory, showed a capacity of generating antigen-specific cytotoxic T lymphocytes (CTLs). In order to enhance an antigen presenting ability of PMDC05, PMDC05 was transduced with CD80 gene by lentiviral vector, which was named as PMDC11. PMDC11 displayed a strong antigen presenting ability even without any stimulation, and by culturing with stimulators such as calcium ionophore PMDC11 gained a more potent antigen presenting ability. Our data suggested PMDC11 could be applied as antigen presenting cells more efficiently in adoptive cellular immunotherapy for tumors and severe infections in comparison with PMDC05.
authors.affiliation: Laboratory of Hematology and Oncology, Graduate School of Health Sciences, Niigata University, Niigata, Japan.
authors.forename: Akie
authors.initials: A
authors.lastname: Yamahira
authors.forename: Miwako
authors.initials: M
authors.lastname: Narita
authors.forename: Kayoko
authors.initials: K
authors.lastname: Ishii
authors.forename: R M Chamila
authors.initials: RM
authors.lastname: Jayathilake
authors.forename: Minami
authors.initials: M
authors.lastname: Iwabuchi
authors.forename: Naoya
authors.initials: N
authors.lastname: Satoh
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Uchiyama
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Taniguchi
authors.forename: Shigeo
authors.initials: S
authors.lastname: Hashimoto
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Kasahara
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Faure
authors.forename: Brooke
authors.initials: B
authors.lastname: Bogan
authors.forename: Jun
authors.initials: J
authors.lastname: Takizawa
authors.forename: Hirohito
authors.initials: H
authors.lastname: Sone
authors.forename: Masuhiro
authors.initials: M
authors.lastname: Takahashi
mesh_terms: D017951:Antigen Presentation; D018122:B7-1 Antigen / Q000235:genetics*; D061207:Calcium Ionophores / Q000494:pharmacology; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D005434:Flow Cytometry; D005822:Genetic Vectors; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D016086:Lentivirus / Q000235:genetics*; D007938:Leukemia; D014161:Transduction, Genetic
chemical_list: D018122:B7-1 Antigen; D061207:Calcium Ionophores