title: Development and characterization of dexamethasone mesylate anchored on multi walled carbon nanotubes.
abstract: Dexamethasone conjugated multi wall carbon nanotubes (DEX-MWCNTs) were developed for controlled delivery of a doxorubicin HCl (DOX) with reduced toxicity. The DEX-MWCNTs were prepared by sequential functionalization of MWCNTs. The DOX was physically loaded onto the purified raw MWCNTs and DEX-MWCNTs at pH 7.4 phosphate buffer solutions (PBS) and evaluated for entrapment efficiency, in vitro release, hemolytic toxicity and ex vivo studies on "A-549" lung epithelial cancer cell line. DOX was efficiently loaded into purified raw and DEX-MWCNTs formulation and the highest entrapment of DOX was found to be 92.6 ± 0.5% in the case of DEX-MWCNTS with good dispersion. In-vitro release of DOX was studied from the DOX/DEX-MWCNTs at pH 5.5 and 7.4 (PBS), which displayed an initial faster followed by sustained release up to 200 h. Further, DOX/DEX-MWCNTs were found to be less hemolytic and more cytotoxic as compared to free DOX on "A-549" lung epithelial cancer cell line.
authors.affiliation: Pharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, Madhya Pradesh, India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Lodhi
authors.forename: Neelesh Kumar
authors.initials: NK
authors.lastname: Mehra
authors.forename: Narendra Kumar
authors.initials: NK
authors.lastname: Jain
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage* / Q000494:pharmacology / Q000633:toxicity; D045744:Cell Line, Tumor; D003692:Delayed-Action Preparations; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000737:chemistry; D004317:Doxorubicin / Q000008:administration & dosage* / Q000494:pharmacology / Q000633:toxicity; D016503:Drug Delivery Systems; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008175:Lung Neoplasms / Q000188:drug therapy / Q000473:pathology; D037742:Nanotubes, Carbon; D013997:Time Factors
chemical_list: D000903:Antibiotics, Antineoplastic; D003692:Delayed-Action Preparations; D037742:Nanotubes, Carbon; D003907:Dexamethasone; D004317:Doxorubicin; C031710:dexamethasone 21-methanesulfonate