title: Monitoring bioremediation of atrazine in soil microcosms using molecular tools.
abstract: Molecular tools in microbial community analysis give access to information on catabolic potential and diversity of microbes. Applied in bioremediation, they could provide a new dimension to improve pollution control. This concept has been demonstrated in the study using atrazine as model pollutant. Bioremediation of the herbicide, atrazine, was analyzed in microcosm studies by bioaugmentation, biostimulation and natural attenuation. Genes from the atrazine degrading pathway atzA/B/C/D/E/F, trzN, and trzD were monitored during the course of treatment and results demonstrated variation in atzC, trzD and trzN genes with time. Change in copy number of trzN gene under different treatment processes was demonstrated by real-time PCR. The amplified trzN gene was cloned and sequence data showed homology to genes reported in Arthrobacter and Nocardioides. Results demonstrate that specific target genes can be monitored, quantified and correlated to degradation analysis which would help in predicting the outcome of any bioremediation strategy.
authors.affiliation: Environmental Genomics Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nehru Marg, Nagpur 440 020, India.
authors.forename: Sneha
authors.initials: S
authors.lastname: Sagarkar
authors.forename: Shinjini
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Aura
authors.initials: A
authors.lastname: Nousiainen
authors.forename: Katarina
authors.initials: K
authors.lastname: Björklöf
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
authors.forename: Kirsten S
authors.initials: KS
authors.lastname: Jørgensen
authors.forename: Atya
authors.initials: A
authors.lastname: Kapley
mesh_terms: D001280:Atrazine / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D004784:Environmental Monitoring / Q000379:methods; D005798:Genes, Bacterial; D006540:Herbicides / Q000378:metabolism*; D008969:Molecular Sequence Data; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D006540:Herbicides; D012987:Soil; D012989:Soil Pollutants; D001280:Atrazine