title: Determination of capsaicinoids in Capsicum species using ultra performance liquid chromatography-mass spectrometry.
abstract: In the present work, a rapid and sensitive ultra performance liquid chromatography-mass spectrometry method has been proposed for the analysis of capsaicinoids (nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin) present in different Capsicum samples. Extraction of capsaicinoids was carried out by liquid-liquid extraction using ethanol as an extracting solvent, while the chromatographic separation was achieved by reversed phase C(18)  column with gradient mobile phase (solvent A: acetonitrile and solvent B: water with 0.1% formic acid). Under the optimum experimental conditions, the linear ranges were 0.5-50 μg/g with correlation coefficient (r(2) ) >0.999 for each capsaicinoids and detection limits were 0.15, 0.05, 0.06, 0.2, and 0.1 μg/g for nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin, respectively. Run-to-run and day-to-day precisions of the method with relative standard deviations <1.5% were achieved for all analyzed capsaicinoids. The robustness of the method was determined by utilizing different injection volumes of the extracts. Furthermore, to validate the system robustness, a run of high number of capsaicinoids present in different varieties of Capsicum samples was performed in this study. All the capsaicinoids were separated in a time of less than 9 min by employing the proposed method.
authors.affiliation: Advanced Materials Research Chair, Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Zeid Abdullah
authors.initials: ZA
authors.lastname: Alothman
authors.forename: Saikh Mohammad
authors.initials: SM
authors.lastname: Wabaidur
authors.forename: Mohammad Rizwan
authors.initials: MR
authors.lastname: Khan
authors.forename: Ayman
authors.initials: A
authors.lastname: Abdel Ghafar
authors.forename: Mohamed Abdelaty
authors.initials: MA
authors.lastname: Habila
authors.forename: Yacine Badjah Hadj
authors.initials: YB
authors.lastname: Ahmed
mesh_terms: D002211:Capsaicin / Q000031:analogs & derivatives / Q000032:analysis*; D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D002211:Capsaicin