title: Catalytic solid substrate-room temperature phosphorimetry for the determination of residual perphenazine based on the electronic effect of rhodamine 6G.
abstract: The rhodamine 6G(+) -perphenazine (Rhod 6G(+) -PPH) compound is formed in the ester-exchange reaction between -OH of PPH and -COOC2 H5 of Rhod 6G(+) . PPH was oxidized to a red compound (PPH') in the presence of K2 S2 O8 . Interestingly, the room temperature phosphorescence (RTP) of Rhod 6G(+) was quenched because the -OH of PPH' reacted with -COOC2 H5 of Rhod 6G(+) -PPH to form Rhod 6G(+) -PPH' and PPH, which decreased the π-electron density (δ) of the carbon atom in the Rhod 6G(+) -PPH' conjugated system and enhanced the nonradiation energy loss of the excited Rhod 6G(+) of the triplet state. The PPH content was directly proportional to the ΔIp of the system. Thus, a new catalytic solid-substrate room temperature phosphorimetry (SSRTP) method was established for the determination of PPH. The method had high sensitivity (the limit of detection was 0.019 fg/spot, corresponding to a concentration of 4.8 × 10(-14)  g/mL; the sampling quantity was 0.40 μL/spot), good selectivity, convenience and speed. The analytical results were in accordance with those of high-performance liquid chromatography (HPLC). The structures of Rhod 6G(+) , PPH and Rhod 6G(+) -PPH were characterized by infrared spectra. The reaction mechanism by which PPH was determined is discussed.
authors.affiliation: Department of Chemistry and Environmental Science, Zhangzhou Normal College, Zhangzhou, 363000, People's Republic of China; Department of Food and Biological Engineering, Zhangzhou Institute of Technology, Zhangzhou, 363000, People's Republic of China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zheng
authors.forename: Ma-Lin
authors.initials: ML
authors.lastname: Cui
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Zhang
authors.forename: Shu-Lian
authors.initials: SL
authors.lastname: Jiang
authors.forename: Li
authors.initials: L
authors.lastname: Jiao
authors.forename: Xuan
authors.initials: X
authors.lastname: Lin
authors.forename: Shao-Qin
authors.initials: SQ
authors.lastname: Lin
authors.forename: Jia-Ming
authors.initials: JM
authors.lastname: Liu
mesh_terms: D002384:Catalysis; D004583:Electrons; D008163:Luminescent Measurements / Q000379:methods*; D015394:Molecular Structure; D010546:Perphenazine / Q000032:analysis*; D012235:Rhodamines / Q000737:chemistry*; D013696:Temperature
chemical_list: D012235:Rhodamines; C026188:rhodamine 6G; D010546:Perphenazine