title: Labeling of hematopoietic stem cells by Tat peptide conjugated quantum dots for cell tracking in mouse body.
abstract: Fluorescent quantum dots have great potential to act as labeling tools in biological research, especially cellular tracking and imaging. Tat peptide conjugated quantum dots were introduced into living human hematopoietic stem cells (HSCs). The internalized quantum dots were assessed by laser confocal microscope and flow cytometer. The quantum dots labeled HSCs were injected intravenously into the tail veins of NOD/SCID beta2M null mice. The tissue collections in the major organs were examined with fluorescence microscope to assess the distribution of transplanted stem cells. HSCs with internalized quantum dots offer a promising approach for stem cell transplantation, which will hold a significant impact on stem cell based therapy for several disorders, especially to cure leukemia in current China.
authors.affiliation: Micro Nano Technology Center, Beijing Institute of Technology, Beijing 100081, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Lei
authors.forename: Haiyang
authors.initials: H
authors.lastname: Tang
authors.forename: Haizheng
authors.initials: H
authors.lastname: Zhong
authors.forename: Haiyan
authors.initials: H
authors.lastname: Xie
authors.forename: Ruibin
authors.initials: R
authors.lastname: Liu
authors.forename: Meifu
authors.initials: M
authors.lastname: Feng
authors.forename: Bingsuo
authors.initials: B
authors.lastname: Zou
mesh_terms: D000818:Animals; D001692:Biological Transport; D015696:Gene Products, tat / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D018613:Microscopy, Confocal; D045663:Quantum Dots
chemical_list: D015696:Gene Products, tat