title: Flow cytometric analysis of kappa and lambda light chain expression in endoscopic biopsy specimens before the diagnosis of B-cell lymphoma.
abstract: Forty-eight patients with gastrointestinal (GI) tract B-cell lymphoma (BCL) were analyzed retrospectively. The diagnosis was based on the histological examination of specimens obtained by endoscopic biopsy. Before the diagnosis was made, single-color flow cytometry was performed to analyze the expression of light chains and B-cell antigens including CD10 in the specimens. Restricted light chain (RLC) expression, a marker of B-cell clonality, was defined as κ and λ ratios of either more than 3.0 or less than 0.5. The specimens from 30 patients (62.5%) showed RLC expression. No RLC expression or RLC expression not examined was divided into two groups : those showing CD10 positivity in more than 20% of cells (4 patients, 8.3%) and those showing no positivity (14 patients, 29.2%). The cell number analyzed in the latter group was significantly smaller than that in the other two groups. Abnormal karyotypes were found in the specimens from 8 patients (16.7%). These results indicate that the flow cytometric analysis of endoscopic biopsy specimens is useful when BCL is suspected if an adequate number of cells are obtained.
authors.affiliation: Department of Internal Medicine (I), Osaka Medical College, Takatsuki, Osaka, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Oka
authors.forename: Kazuo
authors.initials: K
authors.lastname: Muroi
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sato
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Fujiwara
authors.forename: Iekuni
authors.initials: I
authors.lastname: Oh
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Matsuyama
authors.forename: Ken
authors.initials: K
authors.lastname: Ohmine
authors.forename: Takahiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Ozaki
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nagai
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Fukushima
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Fukushima
authors.forename: Akira
authors.initials: A
authors.lastname: Tanaka
authors.forename: Keiya
authors.initials: K
authors.lastname: Ozawa
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D001706:Biopsy; D002452:Cell Count; D004724:Endoscopy; D005260:Female; D041981:Gastrointestinal Tract / Q000378:metabolism* / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007145:Immunoglobulin kappa-Chains / Q000235:genetics* / Q000378:metabolism; D007146:Immunoglobulin lambda-Chains / Q000235:genetics* / Q000378:metabolism; D007621:Karyotyping; D016393:Lymphoma, B-Cell / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015260:Neprilysin / Q000235:genetics; D012189:Retrospective Studies
chemical_list: D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D015260:Neprilysin