title: Metabolite profiling of phenolic and carotenoid contents in tomatoes after moderate-intensity pulsed electric field treatments.
abstract: A metabolite profiling approach was used to study the effect of moderate-intensity pulsed electric field (MIPEF) treatments on the individual polyphenol and carotenoid contents of tomato fruit after refrigeration at 4°C for 24h. The MIPEF processing variables studied were electric field strength (from 0.4 to 2.0kV/cm) and number of pulses (from 5 to 30). Twenty four hours after MIPEF treatments, an increase was observed in hydroxycinnamic acids and flavanones, whereas flavonols, coumaric and ferulic acid-O-glucoside were not affected. Major changes were also observed for carotenoids, except for the 5-cis-lycopene isomer, which remain unchanged after 24h of MIPEF treatments. MIPEF treatments, conducted at 1.2kV/cm and 30 pulses, led to the greatest increases in chlorogenic (152%), caffeic acid-O-glucoside (170%) and caffeic (140%) acids. On the other hand, treatments at 1.2kV/cm and 5 pulses led to maximum increases of α-carotene, 9- and 13-cis-lycopene, which increased by 93%, 94% and 140%, respectively. Therefore, MIPEF could stimulate synthesis of secondary metabolites and contribute to production of tomatoes with high individual polyphenol and carotenoid contents.
authors.affiliation: Nutrition and Food Science Department, University of Barcelona, Barcelona, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Vallverdú-Queralt
authors.forename: Gemma
authors.initials: G
authors.lastname: Oms-Oliu
authors.forename: Isabel
authors.initials: I
authors.lastname: Odriozola-Serrano
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Lamuela-Raventós
authors.forename: Olga
authors.initials: O
authors.lastname: Martín-Belloso
authors.forename: Pedro
authors.initials: P
authors.lastname: Elez-Martínez
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D002338:Carotenoids / Q000032:analysis / Q000378:metabolism*; D004560:Electricity; D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000378:metabolism*; D055442:Metabolome; D059808:Polyphenols / Q000032:analysis / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D059808:Polyphenols; D002338:Carotenoids