title: Enhanced rates of herbicide metabolism in low herbicide-dose selected resistant Lolium rigidum.
abstract: Lolium rigidum is an obligately cross-pollinated, genetically diverse species and an economically important herbicide resistance-prone weed. Our previous work has demonstrated that recurrent selection of initially susceptible L. rigidum populations with low herbicide rates results in rapid herbicide resistance evolution. Here we report on the mechanisms endowing low-dose-selected diclofop-methyl resistance in L. rigidum. Results showed that resistance was not due to target-site ACCase mutations or overproduction, or differential herbicide leaf uptake and translocation. The in vivo de-esterification of diclofop-methyl into phytotoxic diclofop acid was rapid and similar in resistant versus susceptible populations. However, further metabolism of diclofop acid into non-toxic metabolites was always faster in resistant plants than susceptible plants, resulting in up to 2.6-fold lower level of diclofop acid in resistant plants. This corresponded well with up to twofold higher level of diclofop acid metabolites in resistant plants. The major polar metabolites of diclofop acid chromatographically resembled those of wheat, a naturally tolerant species. Clearly, recurrent selection at reduced herbicide rates selected for non-target-site-based enhanced rates of herbicide metabolism, likely involving cytochrome P450 monooxygenases.
authors.affiliation: Australian Herbicide Resistance Initiative (M086) and School of Plant Biology, University of Western Australia, Crawley, WA 6009, Australia. qin.yu@uwa.edu.au
authors.forename: Q
authors.initials: Q
authors.lastname: Yu
authors.forename: H
authors.initials: H
authors.lastname: Han
authors.forename: G R
authors.initials: GR
authors.lastname: Cawthray
authors.forename: S F
authors.initials: SF
authors.lastname: Wang
authors.forename: S B
authors.initials: SB
authors.lastname: Powles
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D005075:Biological Evolution; D002250:Carbon Radioisotopes / Q000032:analysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D055768:Halogenated Diphenyl Ethers / Q000378:metabolism / Q000494:pharmacology*; D053209:Herbicide Resistance / Q000235:genetics*; D006540:Herbicides / Q000378:metabolism / Q000494:pharmacology*; D008129:Lolium / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D009154:Mutation; D010641:Phenotype; D010647:Phenyl Ethers / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*
chemical_list: C529887:2-(4-(2,4-dichlorophenoxy)phenoxy)propionic acid; D002250:Carbon Radioisotopes; D055768:Halogenated Diphenyl Ethers; D006540:Herbicides; D010647:Phenyl Ethers; D010940:Plant Proteins; D011422:Propionates; D003577:Cytochrome P-450 Enzyme System; C012891:dichlorfop-methyl; D000103:Acetyl-CoA Carboxylase