title: Fermentation product of soybean, black bean, and green bean mixture induces apoptosis in a wide variety of cancer cells.
abstract: Previous studies have shown that soybean fermentation products can act as cancer chemoprevention or therapeutic agents. In this study, the anticancer activities of a fermentation product of soybean, black bean, and green bean mixture (BN999) were investigated. We found that BN999 inhibited the growth of human breast cancer AU565 cells and prostate adenocarcinoma PC-3 cells but not that of normal human cells. BN999 induced apoptosis in various human cancer cells but not in normal human cells. BN999 treatment of AU565 cancer cells resulted in activation of calpain and caspase-8, -9, and -3, suggesting that BN999 induces apoptosis via receptor-, mitochondria-, and endoplasmic reticulum-mediated pathways. Finally, we showed that BN999 inhibited the growth of mouse CT-26 colon cancer xenografts in syngenic BALB/c mice without causing obvious side effects. Together, these data suggest that BN999 has potential to be used as a cancer chemoprevention or therapeutic agent.
authors.affiliation: National Taiwan University, Taipei, Taiwan.
authors.forename: Jean-San
authors.initials: JS
authors.lastname: Chia
authors.forename: Jia-Ling
authors.initials: JL
authors.lastname: Du
authors.forename: Ming-Shiou
authors.initials: MS
authors.lastname: Wu
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Hsu
authors.forename: Chun-Pin
authors.initials: CP
authors.lastname: Chiang
authors.forename: Andy
authors.initials: A
authors.lastname: Sun
authors.forename: John Jenn-Yenn
authors.initials: JJ
authors.lastname: Lu
authors.forename: Won-Bo
authors.initials: WB
authors.lastname: Wang
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism; D005285:Fermentation; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D020169:Caspases