title: An HPLC method to detect heme oxygenase activity.
abstract: This unit presents a method to calculate heme oxygenase enzymatic activity from the formation of bilirubin equivalents [biliverdin-Ix alpha (BV) and bilirubin-IX alpha (BR)]. The BV and BR generated in the reaction are separated by reversed-phase HPLC and detected using visible absorbance spectroscopy. Since both metabolites of heme degradation are directly quantifiable, the assay eliminates the requirement for biliverdin reductase supplementation.
authors.affiliation: Southern Illinois University School of Medicine, Springfield, Illinois, USA.
authors.forename: S W
authors.initials: SW
authors.lastname: Ryter
authors.forename: R M
authors.initials: RM
authors.lastname: Tyrrell
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000032:analysis; D001664:Biliverdine / Q000032:analysis; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D002384:Catalysis; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006418:Heme / Q000378:metabolism*; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D006801:Humans; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism; D010084:Oxidation-Reduction
chemical_list: D006418:Heme; D006419:Heme Oxygenase (Decyclizing); D001664:Biliverdine; D001663:Bilirubin