title: Effect of adenosine on injury caused by ischemia and reperfusion in rats: functional and morphologic study.
abstract: To study whether treatment with adenosine (ADO), an agonist of adenosine receptors, attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), we treated rats with ADO (15 mg/kg or saline solution (SS) intravenously before 60 minutes occlusion of the superior mesenteric artery (I) and/or 120 minutes after its release (R). After I or I/R, isolated jejunal segments (2 cm) were mounted in an organ bath to study nerve-mediated contractions stimulated by electrical pulses or KCI with the use of a digital recording system. Thin jejunal slices were stained with hematoxylin and eosin for optical microscopy. Compared with the sham group, jejunal contractions were reduced in I+SS and IR+SS but similar after treatment with ADO (I+ADO and IR+ADO groups). We concluded that rat jejunal enteric nerves were damaged in I+SS and IR+SS but not in the I+ADO and IR+ADO groups. These results suggested that ADO attenuated intestinal dysfunction due to I and R.
authors.affiliation: Department of Pharmacology, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Haddad
authors.forename: R
authors.initials: R
authors.lastname: Miranda-Ferreira
authors.forename: N S A
authors.initials: NS
authors.lastname: Taha
authors.forename: V C
authors.initials: VC
authors.lastname: Maldonado
authors.forename: R R
authors.initials: RR
authors.lastname: Daroz
authors.forename: M O
authors.initials: MO
authors.lastname: Daud
authors.forename: J Lima
authors.initials: JL
authors.lastname: Neto
authors.forename: D A
authors.initials: DA
authors.lastname: Muniz
authors.forename: P C S
authors.initials: PC
authors.lastname: Silva
authors.forename: H P
authors.initials: HP
authors.lastname: Monteiro
authors.forename: D J
authors.initials: DJ
authors.lastname: Fagundes
authors.forename: A
authors.initials: A
authors.lastname: Caricati-Neto
authors.forename: M O
authors.initials: MO
authors.lastname: Taha
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D019610:Cytoprotection; D004195:Disease Models, Animal; D004558:Electric Stimulation; D017615:Enteric Nervous System / Q000187:drug effects / Q000503:physiopathology; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D005769:Gastrointestinal Motility / Q000187:drug effects; D007583:Jejunum / Q000098:blood supply* / Q000187:drug effects* / Q000294:innervation / Q000473:pathology / Q000503:physiopathology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*
chemical_list: D005765:Gastrointestinal Agents; D011189:Potassium Chloride; D000241:Adenosine