title: Retracted: Synergistic anticancer effect of RNAi and photothermal therapy mediated by functionalized single-walled carbon nanotubes.
abstract: Single-walled carbon nanotubes (SWNTs) are special nano-materials which exhibit interesting physical and chemical properties, presenting new opportunities for biomedical research and applications. In this study, we have successfully adopted a novel strategy to chemically functionalize SWNTs with polyethylenimine (PEI) through purification, oxidation, amination and polymerization, which were then bound by DSPE-PEG2000-Maleimide for further conjugation with the tumor targeting NGR (Cys-Asn-Gly-Arg-Cys-) peptide via the maleimide group and sulfhydryl group of cysteine, and finally hTERT siRNA was loaded to obtain a novel tumor targeting siRNA delivery system, designated as SWNT-PEI/siRNA/NGR. The results showed that SWNT-PEI/siRNA/NGR could efficiently cross cell membrane, induced more severe apoptosis and stronger suppression in proliferation of PC-3 cells in vitro. Furthermore, in tumor-bearing mice model the delivery system exhibited higher antitumor activity due to more accumulation in tumor without obvious toxicity in main organs. The combination of RNAi and near-infrared (NIR) photothermal therapy signiﬁcantly enhanced the therapeutic efﬁcacy. In conclusion, SWNT-PEI/siRNA/NGR is a novel and promising anticancer system by combining gene therapy and photothermal therapy.
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jinjin
authors.initials: J
authors.lastname: Shi
authors.forename: Hongling
authors.initials: H
authors.lastname: Zhang
authors.forename: Haixia
authors.initials: H
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Honghong
authors.initials: H
authors.lastname: Wang
authors.forename: Lulu
authors.initials: L
authors.lastname: Li
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Zhang
authors.forename: Chengqun
authors.initials: C
authors.lastname: Chen
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003131:Combined Modality Therapy; D016503:Drug Delivery Systems; D004705:Endocytosis / Q000187:drug effects / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D006979:Hyperthermia, Induced; D007834:Lasers; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D009369:Neoplasms / Q000473:pathology / Q000628:therapy*; D009842:Oligopeptides / Q000494:pharmacology; D009928:Organ Specificity / Q000187:drug effects / Q000528:radiation effects; D010789:Phototherapy; D011094:Polyethyleneimine / Q000138:chemical synthesis / Q000737:chemistry; D034622:RNA Interference / Q000187:drug effects / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C526428:NGR peptide; D037742:Nanotubes, Carbon; D009842:Oligopeptides; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011094:Polyethyleneimine; D019098:Telomerase