title: Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection.
abstract: Recent research suggests that today's condoms are only 85% effective in preventing human immunodeficiency virus (HIV) and other sexually transmitted diseases. In response, there has been a push to develop more effective ways of decreasing the spread of the disease. The new nanotechnology-based condom holds the promise of being more potent than the first-generation products. The preliminary goal of this study was to develop a silver nanoparticles (Ag-NPs)-coated polyurethane condom (PUC) and to investigate its antimicrobial potential including the inactivation of HIV and herpes simplex virus (HSV) infectiousness. The Ag-NPs-coated PUC was characterized by using ultraviolet-visible spectrophotometry, Fourier transform-infrared spectroscopy, high-resolution scanning electron microscopy, and energy-dispersive analysis of X-ray spectroscopy. Nanoparticles were stable on the PUC and not washed away by water. Morphology of the PUC was retained after coating. The NP binding is due to its interaction with the nitrogen atom of the PUC. No significant toxic effects was observed when human HeLa cells, 293T and C8166 T cells were contacted to Ag-NPs-coated PUC for three hours. Interestingly, our results demonstrated that the contact of the Ag-NPs-coated PUC with HIV-1 and HSV-1/2 was able to efficiently inactivate their infectiousness. In an attempt to elucidate the antiviral action of the Ag-NPs, we have demonstrated that the anti-HIV activity was primarily mediated by the Ag-NPs, which are associated with the PUC. In addition, the data showed that both macrophage (M)-tropic and T lymphocyte (T)-tropic strains of HIV-1 were highly sensitive to the Ag-NPs-coated PUC. Furthermore, we also showed that the Ag-NPs-coated PUC was able to inhibit the growth of bacteria and fungi. These results demonstrated that the Ag-NPs-coated PUC is able to directly inactivate the microbe's infectious ability and provides another defense line against these sexually transmitted microbial infections.
authors.affiliation: Laboratory of Molecular Human Retrovirology, Department of Medical Microbiology, University of Manitoba, Winnipeg, MB, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Mohammed Fayaz
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Ao
authors.forename: Morkattu
authors.initials: M
authors.lastname: Girilal
authors.forename: Liyu
authors.initials: L
authors.lastname: Chen
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Xiao
authors.forename: Pt
authors.initials: P
authors.lastname: Kalaichelvan
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Yao
mesh_terms: D000998:Antiviral Agents / Q000008:administration & dosage / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000737:chemistry; D017280:Condoms / Q000382:microbiology*; D015658:HIV Infections / Q000517:prevention & control* / Q000635:transmission; D006367:HeLa Cells; D006558:Herpes Genitalis / Q000517:prevention & control* / Q000635:transmission; D006801:Humans; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D012834:Silver / Q000008:administration & dosage* / Q000737:chemistry; D038301:Virus Inactivation
chemical_list: D000998:Antiviral Agents; D020099:Coated Materials, Biocompatible; D012834:Silver