title: Synaptoneurosome micromethod for fractionation of mouse and human brain, and primary neuronal cultures.
abstract: Brain and primary neuron fractions enriched in synaptic terminals are important tools for neuroscientists in biochemical, neuroanatomical and physiological studies. We describe an annotated updated micro-method for preparing synaptoneurosomes (SNs) enriched in presynaptic and postsynaptic elements. An easy to follow, step-by-step, protocol is provided for making SNs from small amounts of mammalian brain tissue. This includes novel applications for material obtained from human neurosurgical procedures and primary rat neuronal cultures. Our updated method for preparing SNs using smaller amounts of tissue provides a valuable new tool and expands the capabilities of neuroscientists.
authors.affiliation: Department of Neurosurgery, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
authors.forename: Julia W
authors.initials: JW
authors.lastname: Chang
authors.forename: Monica M
authors.initials: MM
authors.lastname: Arnold
authors.forename: Anna
authors.initials: A
authors.lastname: Rozenbaum
authors.forename: Anna
authors.initials: A
authors.lastname: Caputo
authors.forename: Felix E
authors.initials: FE
authors.lastname: Schweizer
authors.forename: My
authors.initials: M
authors.lastname: Huynh
authors.forename: Gary W
authors.initials: GW
authors.lastname: Mathern
authors.forename: Theodore A
authors.initials: TA
authors.lastname: Sarafian
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Watson
mesh_terms: D000293:Adolescent; D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009474:Neurons / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013574:Synaptosomes