title: Impact of recovery from desensitization on acid-sensing ion channel-1a (ASIC1a) current and response to high frequency stimulation.
abstract: BACKGROUND
  Consecutive proton stimulation reduces ASIC1a peak currents leading to silencing of channels.
  
  
  RESULTS
  Kinetic analysis using a fast perfusion system shows that human ASIC1a has two desensitized states with markedly different stabilities.
  
  
  CONCLUSIONS
  High frequency trains of short stimuli prevent desensitization.
  
  
  CONCLUSIONS
  The results predict steady ASIC1a responses to high frequency release of protons as in synaptic transmission. ASIC1a is a neuronal sodium channel activated by external H(+) ions. To date, all the characterization of ASIC1a has been conducted applying long H(+) stimuli lasting several seconds. Such experimental protocols weaken and even silence ASIC1a currents to repetitive stimulation. In this work, we examined ASIC1a currents by methods that use rapid application and removal of H(+). We found that brief H(+) stimuli, <100 ms, even if applied at high frequency, prevent desensitization thereby generate full and steady peak currents of human ASIC1a. Kinetic analysis of recovery from desensitization of hASIC1a revealed two desensitized states: short- and long-lasting with time constants of τ(Ds) ≤0.5 and τ(Dl) = 229 s, while in chicken ASIC1a the two desensitized states have similar values τ(D) 4.5 s. It is the large difference in stability of the two desensitized states that makes hASIC1a desensitization more pronounced and complex than in cASIC1a. Furthermore, recovery from desensitization was unrelated to cytosolic variations in pH, ATP, PIP(2), or redox state but was dependent on the hydrophobicity of key residues in the first transmembrane segment (TM1). In conclusion, brief H(+)-stimuli maintain steady the magnitude of peak currents thereby the ASIC1a channel is well poised to partake in high frequency signals in the brain.
authors.affiliation: Department of Cellular and Molecular Physiology, Yale University, New Haven, CT 06520-8026, USA.
authors.forename: Tianbo
authors.initials: T
authors.lastname: Li
authors.forename: Youshan
authors.initials: Y
authors.lastname: Yang
authors.forename: Cecilia M
authors.initials: CM
authors.lastname: Canessa
mesh_terms: D062565:Acid Sensing Ion Channels / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D051379:Mice; D009865:Oocytes / Q000737:chemistry / Q000378:metabolism; D014981:Xenopus
chemical_list: C568266:ASIC1 protein, human; C568267:ASIC1 protein, mouse; D062565:Acid Sensing Ion Channels