title: Leptospira interrogans shotgun phage display identified LigB as a heparin-binding protein.
abstract: LigB is an adhesin from pathogenic Leptospira that is able to bind to extracellular matrix and is considered a virulence factor. A shotgun phage display genomic library was constructed and used for panning against Heparan Sulfate Proteoglycan (HSPG). A phage clone encoding part of LigB protein was selected in panning experiments and showed specific binding to heparin. To validate the selected clone, fragments of LigB were produced as recombinant proteins and showed affinity to heparin and to mammalian cells. Heparin was also able to reduce the binding of rLB-Ct to mammalian cells. Our data suggests that the glycosaminoglycan moiety of the HSPG is responsible for its binding and could mediate the attachment of the recombinant protein rLB-Ct. Thus, heparin may act as a receptor for Leptospira to colonize and to invade the host tissue.
authors.affiliation: Centro de Biotecnologia, Instituto Butantan, CEP 05503-900 São Paulo, SP, Brazil.
authors.forename: Ana Tung Ching
authors.initials: AT
authors.lastname: Ching
authors.forename: Regiane Degan
authors.initials: RD
authors.lastname: Fávaro
authors.forename: Swiany Silveira
authors.initials: SS
authors.lastname: Lima
authors.forename: Agtha de Alencar Muniz
authors.initials: Ade A
authors.lastname: Chaves
authors.forename: Marcelo Andrade
authors.initials: MA
authors.lastname: de Lima
authors.forename: Helena Bonciani
authors.initials: HB
authors.lastname: Nader
authors.forename: Patrícia A Estima
authors.initials: PA
authors.lastname: Abreu
authors.forename: Paulo Lee
authors.initials: PL
authors.lastname: Ho
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001798:Blood Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation; D006224:Cricetinae; D016207:Cytokines / Q000276:immunology; D006493:Heparin / Q000378:metabolism; D007921:Leptospira interrogans / Q000378:metabolism*; D007922:Leptospirosis / Q000473:pathology / Q000517:prevention & control*; D008214:Lymphocytes / Q000276:immunology; D008647:Mesocricetus; D019151:Peptide Library; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: C062991:AZU1 protein, human; D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D023181:Antimicrobial Cationic Peptides; D001428:Bacterial Vaccines; D001798:Blood Proteins; D002352:Carrier Proteins; D016207:Cytokines; D019151:Peptide Library; D011994:Recombinant Proteins; D006493:Heparin