title: Effect of a pre-freezing treatment with cholesterol-loaded cyclodextrins on boar sperm longevity, capacitation dynamics, ability to adhere to porcine oviductal epithelial cells in vitro and DNA fragmentation dynamics.
abstract: The aim of this work was to examine how a pre-freezing treatment with cholesterol-loaded cyclodextrins (CLC) affects boar sperm longevity, capacitation dynamics, ability to bind to a porcine telomerase-immortalised oviductal epithelial cell line (TERT-OPEC) in vitro and DNA integrity dynamics after freeze-thawing. Although the samples treated with CLC exhibited lower sperm quality than the control samples (P<0.05) immediately after thawing, these differences disappeared (P>0.05) after long-term incubation (26h at 37 or 16°C). Additionally, the CLC-treated spermatozoa underwent similar capacitation and DNA fragmentation dynamics as the control spermatozoa (P>0.05). However, CLC-treated spermatozoa were better able to bind to TERT-OPEC in vitro (P<0.0001). In conclusion, the pre-freezing treatment of boar spermatozoa with CLC enhanced the ability of the spermatozoa to bind to TERT-OPEC in vitro, which could have an effect on the establishment of the sperm reservoir in the ampullary--isthmic junction in vivo. Additionally, frozen-thawed spermatozoa can be stored at 16°C for at least 6h without a significant observable decline in sperm quality, which could be beneficial for the transport of thawed diluted doses of spermatozoa from the laboratory to the farm.
authors.affiliation: Centro de Tecnología Animal - Instituto Valenciano de Investigaciones Agrarias CITA-IVIA. Polígono 'La Esperanza', 100. Apdo. 187. 12400 - Segorbe Castellón, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Tomás
authors.forename: E
authors.initials: E
authors.lastname: Blanch
authors.forename: A
authors.initials: A
authors.lastname: Fazeli
authors.forename: E
authors.initials: E
authors.lastname: Mocé
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism; D003433:Crosses, Genetic; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000009:adverse effects / Q000494:pharmacology*; D003505:Cyclodextrins / Q000009:adverse effects / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D004847:Epithelial Cells / Q000502:physiology; D005187:Fallopian Tubes / Q000502:physiology*; D005260:Female; D008297:Male; D012662:Semen Preservation / Q000009:adverse effects / Q000662:veterinary*; D013030:Spain; D013075:Sperm Capacitation / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D034421:Sus scrofa / Q000502:physiology*
chemical_list: D003451:Cryoprotective Agents; D003505:Cyclodextrins; D002784:Cholesterol