title: CD133(+)/CD44(+)/Oct4(+)/Nestin(+) stem-like cells isolated from Panc-1 cell line may contribute to multi-resistance and metastasis of pancreatic cancer.
abstract: Pancreatic cancer is an aggressive malignant disease. Owing to the lack of early symptoms, accompanied by extensive metastasis and high resistance to chemotherapy, pancreatic adenocarcinoma becomes the fourth leading cause of cancer-related deaths. In this study, we identified a subpopulation of cells isolated from the Panc-1 cell line and named pancreatic cancer stem-like cells. These Panc-1 stem-like cells expressed high levels of CD133/CD44/Oct4/Nestin. Compared to Panc-1 cells, Panc-1 stem-like cells were resistant to gemcitabine and expressed high levels of MDR1; furthermore, Panc-1 stem-like cells have high anti-apoptotic, but weak proliferative potential. These results indicated that Panc-1 stem-like cells, as a novel group, may be a potential major cause of pancreatic cancer multidrug resistance and extensive metastasis.
authors.affiliation: The Affiliated Hospital of Jiangsu University, Zhenjiang 212001, China.
authors.forename: Dongqing
authors.initials: D
authors.lastname: Wang
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
authors.forename: Huiling
authors.initials: H
authors.lastname: Shen
authors.forename: Ruigen
authors.initials: R
authors.lastname: Yin
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Su
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D018432:Drug Resistance, Multiple / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D018960:Hyaluronan Receptors / Q000737:chemistry; D007381:Intermediate Filament Proteins / Q000737:chemistry; D051379:Mice; D016513:Mice, SCID; D009362:Neoplasm Metastasis; D009419:Nerve Tissue Proteins / Q000737:chemistry; D064231:Nestin; D050814:Octamer Transcription Factor-3 / Q000737:chemistry; D010190:Pancreatic Neoplasms / Q000473:pathology* / Q000503:physiopathology; D016133:Polymerase Chain Reaction; D013234:Stem Cells / Q000187:drug effects / Q000473:pathology; D000093542:Gemcitabine
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000964:Antimetabolites, Antineoplastic; D014408:Biomarkers, Tumor; D018960:Hyaluronan Receptors; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D050814:Octamer Transcription Factor-3; D003841:Deoxycytidine; D000093542:Gemcitabine