title: Hairpin priming is better suited than in vitro polyadenylation to generate cDNA for plant miRNA qPCR.
authors.forename: Sajag
authors.initials: S
authors.lastname: Adhikari
authors.forename: Marie
authors.initials: M
authors.lastname: Turner
authors.forename: Senthil
authors.initials: S
authors.lastname: Subramanian
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D009690:Nucleic Acid Conformation; D030821:Plants, Genetically Modified; D026723:Polyadenylation / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs; C034477:RNA primers; D012313:RNA