title: A highly sensitive and temporal visualization system for gaseous ethanol with chemiluminescence enhancer.
abstract: A two-dimensional gaseous ethanol visualization system has been developed and demonstrated using a horseradish peroxidase-luminol-hydrogen peroxide system with high-purity luminol solution and a chemiluminescence (CL) enhancer. This system measures ethanol concentrations as intensities of CL via the luminol reaction. CL was emitted when the gaseous ethanol was injected onto an enzyme-immobilized membrane, which was employed as a screen for two-dimensional gas visualization. The average intensity of CL on the substrate was linearly related to the concentration of standard ethanol gas. These results were compared with the CL intensity of the CCD camera recording image in the visualization system. This system is available for gas components not only for spatial but also for temporal analysis in real time. A high-purity sodium salt HG solution (L-HG) instead of standard luminol solution and an enhancer, eosin Y (EY) solution, were adapted for improvement of CL intensity of the system. The visualization of gaseous ethanol was achieved at a detection limit of 3 ppm at optimized concentrations of L-HG solution and EY.
authors.affiliation: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Arakawa
authors.forename: Eri
authors.initials: E
authors.lastname: Ando
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Miyajima
authors.initials: M
authors.lastname: Kumiko
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kudo
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Saito
authors.forename: Tomoyo
authors.initials: T
authors.lastname: Mitani
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Takahashi
authors.forename: Kohji
authors.initials: K
authors.lastname: Mitsubayashi
mesh_terms: D000431:Ethanol / Q000737:chemistry*; D005740:Gases / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry; D049449:Luminescence; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry*
chemical_list: D005740:Gases; D049408:Luminescent Agents; D000431:Ethanol; D008165:Luminol; D006735:Horseradish Peroxidase