title: Implementation of a dose-response curve for γ-radiation in the Portuguese population by use of the chromosomal aberration assay.
abstract: An in vitro dose-response curve following exposure to γ-radiation was determined at the IST/ITN, by use of the chromosomal aberration assay. This is the first study of this kind carried out among the Portuguese population. Un-irradiated and γ-irradiated peripheral blood lymphocytes from 16 healthy donors were cultured. A total of 22,395 metaphases were analyzed for frequency and distribution of dicentrics and centric rings, as a function of the radiation dose. The dose-response data for dicentrics and dicentrics plus centric rings were fitted by use of a linear-quadratic model: Y(dic)=(0.0011±0.0006)+(0.0105±0.0035)D+(0.0480±0.0019)D(2) and Y(dic+rings)=(0.0011±0.0006)+(0.0095±0.0036)D+(0.0536±0.0020)D(2). Also, calibration curves related to age and gender were determined, but no significant differences were found. Following the establishment of the dose-response curves, a validation experiment was carried out with three individuals. Real and estimated doses, obtained with the dose-response curves, were in agreement. These results give us confidence to apply both dose-response calibration curves in future biological dosimetry requirements.
authors.affiliation: IST/ITN - Instituto Superior Técnico, Universidade Técnica de Lisboa, Estrada Nacional, Bobadela LRS, Portugal.
authors.forename: V
authors.initials: V
authors.lastname: Martins
authors.forename: A C
authors.initials: AC
authors.lastname: Antunes
authors.forename: O
authors.initials: O
authors.lastname: Monteiro Gil
mesh_terms: D000328:Adult; D002869:Chromosome Aberrations / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans; D008875:Middle Aged; D011174:Portugal; D011829:Radiation Dosage; D054928:Validation Studies as Topic; D055815:Young Adult