title: Enhancing sensitivity of liquid chromatographic/ion-trap mass spectrometric determination of jasmonic acid by derivatization with N,N'-dicyclohexylcarbodiimide.
abstract: A simple and sensitive method for the detection of jasmonic acid (JA) by liquid chromatography-ion trap mass spectrometry (LC-IT-MS/MS) after derivatization is described. The highlight of this method was that it involved a rearrangement reaction which was often considered as a side reaction in the formation of amides and esters using N,N'-dicyclohexylcarbodiimide (DCC) as a condensation reagent. While in this study, DCC was used as a derivatization reagent directly. Internal standard dehydrojasmonic acid (DHJA) was used to ensure the accuracy. Derivatives provided good signals corresponding to the protonated molecular ions [M + H](+) and product ions. Sensitivity had been improved 100-fold with the LOD being 0.1 ng mL(-1) in comparison with the LC-IT-MS analysis without derivatization. This proposed method was reliable with good recoveries found in rice floret and wintersweet, allowing quantification in real samples to be more accurate and efficient, thus offering a good way to further study the physiological and biological activities of jasmonic acid in plants.
authors.affiliation: School of Pharmaceutical Sciences, Wuhan University, Wuhan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Qinhua
authors.initials: Q
authors.lastname: Chen
authors.forename: Zilin
authors.initials: Z
authors.lastname: Chen
mesh_terms: D031237:Calycanthaceae / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000032:analysis*; D004024:Dicyclohexylcarbodiimide / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry; D012275:Oryza / Q000737:chemistry; D054883:Oxylipins / Q000032:analysis*
chemical_list: D003517:Cyclopentanes; D007477:Ions; D054883:Oxylipins; D004024:Dicyclohexylcarbodiimide; C011006:jasmonic acid