title: Measurement of aminoacylases.
abstract: The unit describes the assays for aminoacylase activity, with specific emphasis on aminoacylase III. The methods are based on the determination of deacetylated products of the aminoacylase-catalyzed reactions in fluorescence and absorbance assays. The unit also provides methods for isolation of the aminoacylase III from tissues and bacterial or mammalian cells and subsequent purification and analysis.
authors.affiliation: UCLA David Geffen School of Medicine, Los Angeles, California, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Pushkin
authors.forename: Ira
authors.initials: I
authors.lastname: Kurtz
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D001419:Bacteria / Q000201:enzymology; D005591:Chemical Fractionation; D015971:Gene Expression Regulation, Enzymologic; D008322:Mammals; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013054:Spectrophotometry, Atomic / Q000379:methods; D014020:Tissue Extracts
chemical_list: D014020:Tissue Extracts; D000581:Amidohydrolases; C019672:aminoacylase I