title: Genotyping embryonic stem (ES) cells.
abstract: This unit describes a means for isolating DNA from clones cultured in 96-well plates. The extracted DNA can be used for Southern blotting or PCR to verify the genotype of the ES clone.
authors.affiliation: University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: J V
authors.initials: JV
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D053595:Embryonic Stem Cells / Q000166:cytology*; D005838:Genotype; D051379:Mice; D016133:Polymerase Chain Reaction