title: Short communication: Identification of coagulase-negative staphylococcus species from goat milk with the API Staph identification test and with transfer RNA-intergenic spacer PCR combined with capillary electrophoresis.
abstract: Coagulase-negative staphylococci (CNS) are the most commonly isolated bacteria from goat milk, but they have often been identified with phenotypic methods, which may have resulted in misclassification. The aims of this paper were to assess the amount of misclassification of a phenotypic test for identifying CNS species from goat milk compared with transfer RNA intergenic spacer PCR (tDNA-PCR) followed by capillary electrophoresis, and to apply the tDNA-PCR technique on different capillary electrophoresis equipment. Milk samples were collected from 416 does in 5 Californian dairy goat herds on 3 occasions during lactation. In total, 219 CNS isolates were identified at the species level with tDNA-PCR and subjected to the API 20 Staph identification test kit (API Staph; bioMérieux, Durham, NC). If the same species was isolated multiple times from the same udder gland, only the first isolate was used for further analyses, resulting in 115 unique CNS isolates. According to the tDNA-PCR test, the most prevalent CNS species were Staphylococcus epidermidis, Staphylococcus caprae, and Staphylococcus simulans. Typeability with API staph was low (72%). Although the API Staph test was capable of identifying the majority of Staph. epidermidis and Staph. caprae isolates, sensitivity for identification of Staph. simulans was low. The true positive fraction was high for the 3 most prevalent species. It was concluded that the overall performance of API Staph in differentiating CNS species from goat milk was moderate to low, mainly because of the low typeability, and that genotypic methods such as tDNA-PCR are preferred.
authors.affiliation: Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. g.koop@uu.nl
authors.forename: G
authors.initials: G
authors.lastname: Koop
authors.forename: A
authors.initials: A
authors.lastname: De Visscher
authors.forename: C A
authors.initials: CA
authors.lastname: Collar
authors.forename: D A C
authors.initials: DA
authors.lastname: Bacon
authors.forename: E A
authors.initials: EA
authors.lastname: Maga
authors.forename: J D
authors.initials: JD
authors.lastname: Murray
authors.forename: K
authors.initials: K
authors.lastname: Supré
authors.forename: S
authors.initials: S
authors.lastname: De Vliegher
authors.forename: F
authors.initials: F
authors.lastname: Haesebrouck
authors.forename: J D
authors.initials: JD
authors.lastname: Rowe
authors.forename: M
authors.initials: M
authors.lastname: Nielen
authors.forename: T
authors.initials: T
authors.lastname: van Werven
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods / Q000662:veterinary; D005260:Female; D006041:Goats / Q000382:microbiology; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012329:RNA, Bacterial / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D013210:Staphylococcus / Q000145:classification / Q000235:genetics*; D013212:Staphylococcus epidermidis / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012343:RNA, Transfer