title: Impact of SO(2)  on Arabidopsis thaliana transcriptome in wildtype and sulfite oxidase knockout plants analyzed by RNA deep sequencing.
abstract: High concentrations of sulfur dioxide (SO(2) ) as an air pollutant, and its derivative sulfite, cause abiotic stress that can lead to cell death. It is currently unknown to what extent plant fumigation triggers specific transcriptional responses. To address this question, and to test the hypothesis that sulfite oxidase (SO) is acting in SO(2)  detoxification, we compared Arabidopsis wildtype (WT) and SO knockout lines (SO-KO) facing the impact of 600 nl l(-1)  SO(2) , using RNAseq to quantify absolute transcript abundances. These transcriptome data were correlated to sulfur metabolism-related enzyme activities and metabolites obtained from identical samples in a previous study. SO-KO plants exhibited remarkable and broad regulative responses at the mRNA level, especially in transcripts related to sulfur metabolism enzymes, but also in those related to stress response and senescence. Focusing on SO regulation, no alterations were detectable in the WT, whereas in SO-KO plants we found up-regulation of two splice variants of the SO gene, although this gene is not functional in this line. Our data provide evidence for the highly specific coregulation between SO and sulfur-related enzymes like APS reductase, and suggest two novel candidates for involvement in SO(2)  detoxification: an apoplastic peroxidase, and defensins as putative cysteine mass storages.
authors.affiliation: Institut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstraße 1, D-38106, Braunschweig, Germany.
authors.forename: Domenica
authors.initials: D
authors.lastname: Hamisch
authors.affiliation: Institut für Forstbotanik und Baumphysiologie, Professur für Baumphysiologie, Albert-Ludwigs-Universität Freiburg, Georges-Köhler Allee 53/54, D-79085, Freiburg, Germany.
authors.forename: Dörte
authors.initials: D
authors.lastname: Randewig
authors.affiliation: Institut für Biochemie der Pflanzen, Heinrich-Heine-Universität, Universitätsstraße 1, D-40225, Düsseldorf, Germany.
authors.forename: Simon
authors.initials: S
authors.lastname: Schliesky
authors.affiliation: Institut für Biochemie der Pflanzen, Heinrich-Heine-Universität, Universitätsstraße 1, D-40225, Düsseldorf, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Bräutigam
authors.affiliation: Institut für Biochemie der Pflanzen, Heinrich-Heine-Universität, Universitätsstraße 1, D-40225, Düsseldorf, Germany.
authors.forename: Andreas P M
authors.initials: APM
authors.lastname: Weber
authors.affiliation: Genome Analytics, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, D-38124, Braunschweig, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Geffers
authors.affiliation: Institut für Forstbotanik und Baumphysiologie, Professur für Baumphysiologie, Albert-Ludwigs-Universität Freiburg, Georges-Köhler Allee 53/54, D-79085, Freiburg, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Herschbach
authors.affiliation: Institut für Forstbotanik und Baumphysiologie, Professur für Baumphysiologie, Albert-Ludwigs-Universität Freiburg, Georges-Köhler Allee 53/54, D-79085, Freiburg, Germany.|King Saud University, PO Box 2454, Riyadh, 11451, Saudi Arabia.
authors.forename: Heinz
authors.initials: H
authors.lastname: Rennenberg
authors.affiliation: Institut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstraße 1, D-38106, Braunschweig, Germany.
authors.forename: Ralf R
authors.initials: RR
authors.lastname: Mendel
authors.affiliation: Institut für Pflanzenbiologie, Technische Universität Braunschweig, Humboldtstraße 1, D-38106, Braunschweig, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Hänsch
mesh_terms: D000393:Air Pollutants / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D023082:Defensins / Q000235:genetics; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D055786:Gene Knockout Techniques; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000235:genetics; D030821:Plants, Genetically Modified; D017423:Sequence Analysis, RNA / Q000379:methods*; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D050876:Sulfite Oxidase / Q000235:genetics* / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism; D013458:Sulfur Dioxide / Q000494:pharmacology*; D059467:Transcriptome
chemical_list: D000393:Air Pollutants; D029681:Arabidopsis Proteins; D023082:Defensins; D004798:Enzymes; D013458:Sulfur Dioxide; D013455:Sulfur; D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors; D050876:Sulfite Oxidase; C019618:adenylylsulfate reductase