title: Flagella from F18+Escherichia coli play a role in adhesion to pig epithelial cell lines.
abstract: F18 fimbriae and toxins produced by F18 fimbriae-carrying Escherichia coli (E. coli) strains are known virulence factors responsible for post-weaning diarrhea (PWD) and edema disease (ED). In this study, we showed that fliC isogenic mutants constructed in two reference wild-type F18 fimbriae (F18+) E. coli were markedly impaired in adherence in vitro cell models (p < 0.05). Flagella purified from F18+E. coli could directly bind to cultured piglet epithelial cells and block adherence of F18+E. coli to cells when pre-incubated. In addition, the F18+E. coli fliC deletion mutants up-regulated the expression of type I fimbriae produced by F18+E. coli strains. These results demonstrated that expression of flagella is essential for the adherence of F18+E. coli in vitro.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Ministry of Education Key Lab for Avian Preventive Medicine, China.
authors.forename: Qiangde
authors.initials: Q
authors.lastname: Duan
authors.forename: Mingxu
authors.initials: M
authors.lastname: Zhou
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Zhu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Wenbin
authors.initials: W
authors.lastname: Bao
authors.forename: Shenglong
authors.initials: S
authors.lastname: Wu
authors.forename: Xiaosai
authors.initials: X
authors.lastname: Ruan
authors.forename: Weiping
authors.initials: W
authors.lastname: Zhang
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism; D005407:Flagella / Q000235:genetics / Q000502:physiology*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D029968:Escherichia coli Proteins; C113053:FedA protein, E coli; D036781:Fimbriae Proteins