title: Noble polymeric surface conjugated with zwitterionic moieties and antibodies for the isolation of exosomes from human serum.
abstract: New zwitterionic polymer-coated immunoaffinity beads were developed to resist nonspecific protein adsorption from undiluted human serum for diagnostic applications of exosomes. A zwitterionic sulfobetaine monomer with an amine functional group was employed for simple surface chemistry and antifouling properties. An exosomal biomarker protein, epithelial cell adhesion molecule (EpCAM), was selected as a target molecule in this work. The beads were coated with polyacrylic acids (PAA) for increasing biorecognition sites, and protein G was then conjugated with carboxylic acid groups on the surfaces for controlling EpCAM antibody orientation. The remaining free carboxylic acid groups were modified with sulfobetaine moieties, and anti-EpCAM antibody was finally introduced. The amount of anti-EpCAM on the beads was increased by 40% when compared with PAA-uncoated beads. The surfaces of the beads exhibited near-net-zero charge, and nonspecific protein adsorption was effectively suppressed by sulfobetaine moieties. EpCAM was captured from undiluted human serum with almost the same degree of efficiency as from PBS buffer solution using the newly developed immunoaffinity beads.
authors.affiliation: Bio Research Center, Samsung Advanced Institute of Technology (SAIT), Mt. 14-1, Nongseo-dong, Yongin-si, 446-712, Gyeonggi-do, South Korea.
authors.forename: Gahee
authors.initials: G
authors.lastname: Kim
authors.forename: Chang Eun
authors.initials: CE
authors.lastname: Yoo
authors.forename: Myoungsoon
authors.initials: M
authors.lastname: Kim
authors.forename: Hyun Ju
authors.initials: HJ
authors.lastname: Kang
authors.forename: Donghyun
authors.initials: D
authors.lastname: Park
authors.forename: Myoyong
authors.initials: M
authors.lastname: Lee
authors.forename: Nam
authors.initials: N
authors.lastname: Huh
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology; D002458:Cell Fractionation / Q000379:methods*; D000071858:Epithelial Cell Adhesion Molecule; D055354:Exosomes / Q000378:metabolism*; D006801:Humans; D059346:Magnets / Q000737:chemistry; D008863:Microspheres; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D000906:Antibodies; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; C006903:carbopol 940