title: [Construction and application of a multiplex PCR system for genotyping of human red blood cell antigens].
abstract: OBJECTIVE
  A reliable method for genotyping blood group antigens Dib, k, Jsb1910 and Jsb2019 was developed. Through screening for rare blood types, the National Rare Blood Bank of China may be enriched.
  
  
  METHODS
  The controls for allele detection of blood groups Dib, k, Jsb1910 and Jsb2019 were prepared via polymerase chain reaction (PCR)-mediated gene site-directed mutagenesis (SDM) technique. Sequence-specific primers were designed according to known single nucleotide polymorphism (SNP) sites of alleles of blood groups antigens Dib, k, Jsb1910 and Jsb2019, a multiplex PCR system was developed by optimizing PCR reaction system. And 4190 random healthy donors samples were screened for the blood group antigens.
  
  
  RESULTS
  Using SDM technique, controls for alleles in blood group Dib, k, Jsb1910 and Jsb2019 were successfully generated. And a multiplex PCR system for genotyping above blood groups was developed. After verification, the system has performed with good stability and reproducibility. Two Di (b-) samples have been discovered from 4190 samples, no k- and Js(b-) sample was found.
  
  
  CONCLUSIONS
  Multiplex PCR features rapid detection, high throughput and low cost, and can be used for screening for donors of rare blood types. Information of donors may be registered in a database, which in turn can help those with rare blood types or require long-term blood transfusion to obtain matched blood, thereby reduce the adverse reactions of blood transfusion.
authors.affiliation: Blood Group Reference Laboratory, Shanghai Institute of Blood Transfusion, Shanghai Blood Center, Shanghai, People's Republic of China.
authors.forename: Huan-huan
authors.initials: HH
authors.lastname: Gao
authors.forename: Yun-lei
authors.initials: YL
authors.lastname: He
authors.forename: Lu-yi
authors.initials: LY
authors.lastname: Ye
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.forename: Zhong-hui
authors.initials: ZH
authors.lastname: Guo
authors.forename: Zi-yan
authors.initials: ZY
authors.lastname: Zhu
authors.forename: Yong-ming
authors.initials: YM
authors.lastname: Zhu
mesh_terms: D001789:Blood Group Antigens / Q000235:genetics*; D004912:Erythrocytes / Q000276:immunology*; D060005:Genotyping Techniques; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D016297:Mutagenesis, Site-Directed; D020641:Polymorphism, Single Nucleotide
chemical_list: D001789:Blood Group Antigens