title: Cortical neurovascular coupling driven by stimulation of channelrhodopsin-2.
abstract: While functional imaging is widely used in studies of the brain, how well the hemodynamic signal represents the underlying neural activity is still unclear. And there is a debate on whether hemodynamic signal is more tightly related to synaptic activity or action potentials. This study intends to address these questions by examining neurovascular coupling driven by pyramidal cells in the motor cortex of rats. Pyramidal cells in the motor cortex of rats were selectively transduced with the light sensitive cation channel channelrhodopsin-2 (ChR2). Electrophysiological recordings and optical intrinsic signal imaging were performed simultaneously and synchronously to capture the neural activity and hemodynamics induced by optical stimulation of ChR2-expressing pyramidal cells. Our results indicate that both synaptic activity (local field potential, LFP) and action potentials (multi-unit activity, MUA) are tightly related to hemodynamic signals. While LFPs in γ band are better in predicting hemodynamic signals elicited by short stimuli, MUA has better predictions to hemodynamic signals elicited by long stimuli. Our results also indicate that strong nonlinearity exists in neurovascular coupling.
authors.affiliation: The J Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, United States of America.
authors.forename: Lijun
authors.initials: L
authors.lastname: Ji
authors.forename: Junli
authors.initials: J
authors.lastname: Zhou
authors.forename: Rabia
authors.initials: R
authors.lastname: Zafar
authors.forename: Svetlana
authors.initials: S
authors.lastname: Kantorovich
authors.forename: Ruixin
authors.initials: R
authors.lastname: Jiang
authors.forename: Paul R
authors.initials: PR
authors.lastname: Carney
authors.forename: Huabei
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000200:Action Potentials; D000818:Animals; D000075402:Channelrhodopsins; D015870:Gene Expression; D006439:Hemodynamics; D008297:Male; D009044:Motor Cortex / Q000098:blood supply*; D010108:Oxyhemoglobins / Q000378:metabolism; D010775:Photic Stimulation; D017966:Pyramidal Cells / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D055366:Synaptic Potentials; D009435:Synaptic Transmission
chemical_list: D000075402:Channelrhodopsins; D010108:Oxyhemoglobins