title: Comparison of different alkali-based pretreatments of corn stover for improving enzymatic saccharification.
abstract: Corn stover was treated with NaOH, NaOH+anthraquinone (AQ), NaOH+Na(2)SO(3) (alkaline), NaOH+Na(2)SO(3) (neutral), and NaOH+Na(2)S, respectively. The treated corn stover was subjected to hydrolysis with cellulase (20 FPU/g dry biomass) and β-glucosidase (10I U/g dry biomass). Compared with other pretreatment methods, alkaline sodium sulfite pretreatment (ASSP) at a relatively low temperature of 140°C provided for the best lignin removal of about 92%. After ASSP with 10 wt.% of the total alkali charge (Na(2)SO(3):NaOH=1:1) at 140°C for 30 min and subsequent enzymatic hydrolysis, a total sugar yield of 78.2% was obtained on the basis of the amount of glucose and xylose released from raw corn stover. This yield was 24.0% higher than that achieved with NaOH only under the same conditions. Therefore, the supplement of Na(2)SO(3) in alkali pretreatment can facilitate delignification and significantly improve the enzymatic saccharification.
authors.affiliation: Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.forename: Haisong
authors.initials: H
authors.lastname: Wang
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Guang
authors.initials: G
authors.lastname: Yu
authors.forename: Xindong
authors.initials: X
authors.lastname: Mu
mesh_terms: D000468:Alkalies / Q000737:chemistry*; D002241:Carbohydrates / Q000138:chemical synthesis*; D002480:Cellulase / Q000737:chemistry*; D004789:Enzyme Activation; D006868:Hydrolysis; D007220:Industrial Waste / Q000517:prevention & control*; D035261:Plant Components, Aerial / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry*
chemical_list: D000468:Alkalies; D002241:Carbohydrates; D007220:Industrial Waste; D001617:beta-Glucosidase; D002480:Cellulase