title: [Effect of total flavonoids of tartary buckwheat on palmitic acid-induced NO synthesis in EA.hy926 cells].
abstract: OBJECTIVE
  To observe the effects of total flavonoids of tartary buckwheat on NO synthesis in EA.hy926 cells induced by palmitic acid.
  
  
  METHODS
  EA.hy926 cells were cultured in vitro and randomly divided into control group, palmitic acid-induced insulin resistance group, total flavonoids of tartary buckwheat group and metformin group. The content of NO in supernatant was detected by nitrate reductase. The eNOS mRNA and protein expression levels were determined by RT-PCR and Western blotting, respectively.
  
  
  RESULTS
  Compared with control group, the NO content in supernatant and the expression levels of eNOS mRNA and protein were significantly lower in insulin resistance group (P<0.05). Compared with insulin resistance group, the NO content in supernatant, as well as the eNOS mRNA and protein expression markedly increased in both total flavonoids of tartary buckwheat group and metformin group (P<0.05), but there was no significant difference between the latter two groups (P>0.05).
  
  
  CONCLUSIONS
  Total flavonoids of tartary buckwheat effectively promotes the expression of eNOS mRNA and protein in endothelial cells under palmitic acid stimulation, thereby contributing to the NO synthesis.
authors.affiliation: Department of Combined Chinese and Western Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang, China. yangxuefeng583@sina.com
authors.forename: Xue-feng
authors.initials: XF
authors.lastname: Yang
authors.forename: Rui-peng
authors.initials: RP
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Dan-yu
authors.initials: DY
authors.lastname: Zhao
authors.forename: Yan-jie
authors.initials: YJ
authors.lastname: Wang
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
mesh_terms: D002460:Cell Line; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism*; D019612:Fagopyrum / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D009569:Nitric Oxide / Q000096:biosynthesis*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D019308:Palmitic Acid / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D005419:Flavonoids; D012333:RNA, Messenger; D019308:Palmitic Acid; D009569:Nitric Oxide; D052250:Nitric Oxide Synthase Type III