title: Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic study.
abstract: OBJECTIVE
  This study evaluated subcutaneous tissue response to Aroeira (Myracrodruon urundeuva) extract employing edemogenic and histological analyses.
  
  
  METHODS
  Test groups consisted of aqueous and ethanolic Aroeira extracts and saline (control). For edema quantification, 18 rats received an intravenous injection of Evan's Blue. After 30 min, the extracts and saline were injected on the dorsum of the rats, which were then sacrificed after 3 and 6 h. Readings were performed in a spectrophotometer. For subcutaneous implantation, 30 rats received a polyethylene tube containing the extracts on their dorsum and then they were killed after 7 and 28 days. The samples were processed for histological analysis and evaluated with a light microscope. The inflammatory infiltrate was quantified.
  
  
  RESULTS
  There were no statistically significant differences between aqueous extract and saline groups in relation to edema quantification in the different periods (p>0.05). Ethanolic solution resulted in more edema independently of the experimental period (p<0.05). Histological analysis showed similar results on the 7-day period for the 3 groups. There was a notable reduction on inflammatory cell number for saline and aqueous extract groups at 28 days.
  
  
  CONCLUSIONS
  The aqueous extract showed biocompatible properties similar to those of saline.
authors.affiliation: Department of Endodontics, Araçatuba School of Dentistry, Univ. Estadual Paulista, Araçatuba, SP, Brazil.
authors.forename: Alessandra Cury
authors.initials: AC
authors.lastname: Machado
authors.forename: Eloi
authors.initials: E
authors.lastname: Dezan Junior
authors.forename: João Eduardo
authors.initials: JE
authors.lastname: Gomes-Filho
authors.forename: Luciano Tavares Angelo
authors.initials: LT
authors.lastname: Cintra
authors.forename: Denise Belucio
authors.initials: DB
authors.lastname: Ruviére
authors.forename: Renata
authors.initials: R
authors.lastname: Zoccal
authors.forename: Carla Andreotti
authors.initials: CA
authors.lastname: Damante
authors.forename: Elerson Gaetti
authors.initials: EG
authors.lastname: Jardim Junior
mesh_terms: D027926:Anacardiaceae / Q000633:toxicity*; D000818:Animals; D004487:Edema / Q000188:drug therapy / Q000209:etiology; D007249:Inflammation / Q000188:drug therapy; D010936:Plant Extracts / Q000633:toxicity*; D010946:Plants, Medicinal / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
chemical_list: D010936:Plant Extracts; D012965:Sodium Chloride