title: Gold nanoparticles conjugated with monosaccharide-modified peptide for lectin detection.
abstract: Gold nanoparticles (GNPs) conjugated with monosaccharide-modified peptides have been developed as optical probes for lectin detection. Mannose-modified peptides were designed and conjugated with GNPs. The GNPs with mannose-modified peptide showed remarkable red shift of absorption maximum due to the aggregation with concanavalin A (ConA), a mannose-binding lectin. The aggregation activity of glycopeptide-modified GNPs with ConA depended on the amino acid sequence around the mannose unit of glycopeptides.
authors.affiliation: Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta-cho 4259 B-40, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsutsumi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohkusa
authors.forename: Hyejin
authors.initials: H
authors.lastname: Park
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Hideya
authors.initials: H
authors.lastname: Yuasa
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Mihara
mesh_terms: D003208:Concanavalin A / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D010455:Peptides; D003208:Concanavalin A; D006046:Gold; D008358:Mannose