title: A mouse model for HIV-1 entry.
abstract: Passive transfer of neutralizing antibodies against HIV-1 can prevent infection in macaques and seems to delay HIV-1 rebound in humans. Anti-HIV antibodies are therefore of great interest for vaccine design. However, the basis for their in vivo activity has been difficult to evaluate systematically because of a paucity of small animal models for HIV infection. Here we report a genetically humanized mouse model that incorporates a luciferase reporter for rapid quantitation of HIV entry. An antibody's ability to block viral entry in this in vivo model is a function of its bioavailability, direct neutralizing activity, and effector functions.
authors.affiliation: Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
authors.forename: John
authors.initials: J
authors.lastname: Pietzsch
authors.forename: Henning
authors.initials: H
authors.lastname: Gruell
authors.forename: Stylianos
authors.initials: S
authors.lastname: Bournazos
authors.forename: Bridget M
authors.initials: BM
authors.lastname: Donovan
authors.forename: Florian
authors.initials: F
authors.lastname: Klein
authors.forename: Ron
authors.initials: R
authors.lastname: Diskin
authors.forename: Michael S
authors.initials: MS
authors.lastname: Seaman
authors.forename: Pamela J
authors.initials: PJ
authors.lastname: Bjorkman
authors.forename: Jeffrey V
authors.initials: JV
authors.lastname: Ravetch
authors.forename: Alexander
authors.initials: A
authors.lastname: Ploss
authors.forename: Michel C
authors.initials: MC
authors.lastname: Nussenzweig
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D000914:Antibodies, Viral / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D004195:Disease Models, Animal; D015658:HIV Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008251:Macaca; D051379:Mice; D008822:Mice, Transgenic; D053586:Virus Internalization
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral