title: Extraction conditions of Antheraea mylitta sericin with high yields and minimum molecular weight degradation.
abstract: Although the technique for extracting the Bombyx mori sericin has been extensively known, the extraction of sericin from wild-silkworm cocoons is not yet standardized. The aim of this study was to find the optimal conditions for the extraction of sericin from Antheraea mylitta cocoons, with high yields and minimum degradation. We attempted to apply various protocols for the extraction of the A. mylitta sericin (AmS). Among these, we found that the extraction of AmS with a sodium carbonate solution exhibited the highest yield except the conventional soap-alkali extraction. To find the optimal conditions for the AmS extraction with the sodium carbonate, we changed the concentration of sodium carbonate and the treatment time. With an increase in the sodium carbonate concentration and the extraction time, the yield of AmS increased, but the molecular weight (MW) of AmS decreased. Considering the yield, molecular weight distribution (MWD) and amino acid composition of AmS, we suggest that the optimal conditions for the AmS extraction require treatment with 0.02 M sodium carbonate and boiling for 60 min.
authors.affiliation: Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Haesung
authors.initials: H
authors.lastname: Yun
authors.forename: Hanjin
authors.initials: H
authors.lastname: Oh
authors.forename: Moo Kon
authors.initials: MK
authors.lastname: Kim
authors.forename: Hyo Won
authors.initials: HW
authors.lastname: Kwak
authors.forename: Jeong Yun
authors.initials: JY
authors.lastname: Lee
authors.forename: In Chul
authors.initials: IC
authors.lastname: Um
authors.forename: Shyam Kumar
authors.initials: SK
authors.lastname: Vootla
authors.forename: Ki Hoon
authors.initials: KH
authors.lastname: Lee
mesh_terms: D000818:Animals; D009036:Moths / Q000737:chemistry*; D059748:Proteolysis; D047030:Sericins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D047030:Sericins