title: Two clinical isolates of Candida glabrata exhibiting reduced sensitivity to amphotericin B both harbor mutations in ERG2.
abstract: Two novel isolates of Candida glabrata exhibiting reduced sensitivity to amphotericin B (MIC, 8 μg ml(-1)) were found to be ERG2 mutants, wherein Δ(8)-sterol intermediates comprised >90% of the total cellular sterol fraction. Both harbored an alteration at Thr(121) in ERG2; the corresponding residue (Thr(119)) in Saccharomyces cerevisiae is essential for sterol Δ8-Δ7 isomerization. This constitutes the first report of C. glabrata harboring mutations in ERG2 and exhibiting reduced sensitivity to amphotericin B.
authors.affiliation: Institute of Life Science, Swansea University, Swansea, Wales, UK.
authors.forename: Claire M
authors.initials: CM
authors.lastname: Hull
authors.forename: Oliver
authors.initials: O
authors.lastname: Bader
authors.forename: Josie E
authors.initials: JE
authors.lastname: Parker
authors.forename: Michael
authors.initials: M
authors.lastname: Weig
authors.forename: Uwe
authors.initials: U
authors.lastname: Gross
authors.forename: Andrew G S
authors.initials: AG
authors.lastname: Warrilow
authors.forename: Diane E
authors.initials: DE
authors.lastname: Kelly
authors.forename: Steven L
authors.initials: SL
authors.lastname: Kelly
mesh_terms: D000595:Amino Acid Sequence; D000666:Amphotericin B / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology; D041221:Candida glabrata / Q000187:drug effects* / Q000235:genetics*; D002177:Candidiasis / Q000382:microbiology*; D025141:Drug Resistance, Fungal / Q000235:genetics*; D015725:Fluconazole / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009154:Mutation; D011743:Pyrimidines / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics; D013251:Steroid Isomerases / Q000235:genetics*; D013261:Sterols / Q000737:chemistry; D014230:Triazoles / Q000494:pharmacology; D065819:Voriconazole
chemical_list: D000935:Antifungal Agents; D001393:Azoles; D005656:Fungal Proteins; D011743:Pyrimidines; D013261:Sterols; D014230:Triazoles; D000666:Amphotericin B; D015725:Fluconazole; D013251:Steroid Isomerases; D065819:Voriconazole