title: In-depth proteome mining of cultured Catharanthus roseus cells identifies candidate proteins involved in the synthesis and transport of secondary metabolites.
abstract: Madagascar periwinkle (Catharanthus roseus) is the major source of terpenoid indole alkaloids, such as vinblastine or vincristine, used as natural drugs against various cancers. In this study, we have extensively analyzed the proteome of cultured C. roseus cells. Comparison of the proteomes of two independent cell lines with different terpenoid indole alkaloid metabolism by 2D-DIGE revealed 358 proteins that differed quantitatively by at least a twofold average ratio. Of these, 172 were identified by MS; most corresponded to housekeeping proteins. Less abundant proteins were identified by LC separation of tryptic peptides of proteins from one of the lines. We identified 1663 proteins, most of which are housekeeping proteins or involved in primary metabolism. However, 63 enzymes potentially involved in secondary metabolism were also identified, of which 22 are involved in terpenoid indole alkaloid biosynthesis and 16 are predicted transporters putatively involved in secondary metabolite transport. About 30% of the proteins identified have an unclear or unknown function, indicating important gaps in knowledge of plant metabolism. This study is an important step toward elucidating the proteome of C. roseus, which is critical for a better understanding of how this plant synthesizes terpenoid indole alkaloids.
authors.affiliation: Institut des Sciences de la Vie, Université catholique de Louvain, Louvain-la-Neuve, Belgium.
authors.forename: Antoine
authors.initials: A
authors.lastname: Champagne
authors.forename: Heiko
authors.initials: H
authors.lastname: Rischer
authors.forename: Kirsi-Marja
authors.initials: KM
authors.lastname: Oksman-Caldentey
authors.forename: Marc
authors.initials: M
authors.lastname: Boutry
mesh_terms: D030008:Catharanthus / Q000201:enzymology / Q000378:metabolism*; D002460:Cell Line; D039821:Monoterpenes / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism; D046948:Secologanin Tryptamine Alkaloids / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D039821:Monoterpenes; D010940:Plant Proteins; D020543:Proteome; D046948:Secologanin Tryptamine Alkaloids