title: Selective determination of cholesterol based on cholesterol oxidase-alkaline phosphatase bienzyme electrode.
abstract: A bienzyme electrode for the determination of cholesterol was prepared by the co-immobilization of cholesterol oxidase and alkaline phosphatase (ALP) on the carbon nanotubes modified electrode surface. The parameters influenced the performance of the bienzyme electrode such as the pH of the base solution; the concentration of DPP and the incubation time, were optimized. A linear calibration graph in the 0.05-2.0 mM concentration range with the sensitivity of 4.65 μA mM(-1) was obtained. In addition, this work provides a new interferent-depleted method and universal protocol for the design of multi-enzyme biosensors.
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecular Chemistry, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, P. R. China.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Zhang
authors.forename: Ruixiao
authors.initials: R
authors.lastname: Liu
authors.forename: Jianbin
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism*; D015374:Biosensing Techniques; D002784:Cholesterol / Q000032:analysis*; D002789:Cholesterol Oxidase / Q000737:chemistry / Q000378:metabolism*; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009943:Organophosphorus Compounds / Q000737:chemistry; D019800:Phenol / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D009943:Organophosphorus Compounds; D019800:Phenol; D002784:Cholesterol; D002789:Cholesterol Oxidase; D000469:Alkaline Phosphatase