title: Methylparaben concentration in commercial Brazilian local anesthetics solutions.
abstract: OBJECTIVE
  To detect the presence and concentration of methylparaben in cartridges of commercial Brazilian local anesthetics.
  
  
  METHODS
  Twelve commercial brands (4 in glass and 8 in plastic cartridges) of local anesthetic solutions for use in dentistry were purchased from the Brazilian market and analyzed. Different lots of the commercial brands were obtained in different Brazilian cities (Piracicaba, Campinas and São Paulo). Separation was performed using high performance liquid chromatography (HPLC) with UV-Vis detector. The mobile phase used was acetonitrile:water (75:25 - v/v), pH 4.5, adjusted with acetic acid at a flow rate of 1.0 ml.min-1.
  
  
  RESULTS
  When detected in the solutions, the methylparaben concentration ranged from 0.01% (m/v) to 0.16% (m/v). One glass and all plastic cartridges presented methylparaben.
  
  
  CONCLUSIONS
  1. Methylparaben concentration varied among solutions from different manufacturers, and it was not indicated in the drug package inserts; 2. Since the presence of methylparaben in dental anesthetics is not regulated by the Brazilian National Health Surveillance Agency (ANVISA) and this substance could cause allergic reactions, it is important to alert dentists about its possible presence.
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, University of Campinas, Campinas, SP, Brazil.
authors.forename: Gustavo Henrique Rodriguez da
authors.initials: GH
authors.lastname: Silva
authors.forename: Carla Beatriz Grespan
authors.initials: CB
authors.lastname: Bottoli
authors.forename: Francisco Carlos
authors.initials: FC
authors.lastname: Groppo
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Volpato
authors.forename: José
authors.initials: J
authors.lastname: Ranali
authors.forename: Juliana Cama
authors.initials: JC
authors.lastname: Ramacciato
authors.forename: Rogério Heládio Lopes
authors.initials: RH
authors.lastname: Motta
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000779:Anesthetics, Local / Q000737:chemistry*; D001938:Brazil; D004342:Drug Hypersensitivity / Q000209:etiology; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D010226:Parabens / Q000032:analysis*; D011310:Preservatives, Pharmaceutical / Q000032:analysis*; D012996:Solutions / Q000737:chemistry; D013997:Time Factors
chemical_list: D000097:Acetonitriles; D000779:Anesthetics, Local; D007202:Indicators and Reagents; D010226:Parabens; D011310:Preservatives, Pharmaceutical; D012996:Solutions; C015358:methylparaben; D019342:Acetic Acid; C032159:acetonitrile