title: The combination of EPA+DHA and low-dose aspirin ingestion reduces platelet function acutely whereas each alone may not in healthy humans.
abstract: BACKGROUND
  Aspirin's effectiveness in reducing cardiovascular disease events is inadequate in some individuals, a phenomenon termed aspirin "resistance". The hypothesis that combining low dose aspirin with eicosapentaenoic acid and docosahexaenoic acid (EPA+DHA) reduces platelet function in the acute setting has not been investigated.
  
  
  METHODS
  We conducted a clinical trial of EPA+DHA and aspirin ingestion in healthy adults. Fasting blood samples were drawn at baseline and 4 h after supplementation with EPA/DHA (3.4 g/d), aspirin (81 mg), and both. Platelet function was measured using the Platelet Function Analyzer-100 (PFA-100). Plasma lysophosphatidylcholine (LPC), lysophosphatidic acid (LPA), autotaxin, angiogenesis activators, and cytokines were measured.
  
  
  RESULTS
  Platelet function decreased with the combination of aspirin+EPA/DHA (p=0.03) but not with either alone (p>0.05). EPA-LPC increased (p=0.002).
  
  
  CONCLUSIONS
  Our results demonstrate that a potentially beneficial effect on platelet function occurred within 4h after ingestion of low-dose aspirin and EPA+DHA in healthy adults.
authors.affiliation: Department of Community and Preventive Medicine, The University of Rochester School of Medicine and Dentistry, Box 644, Rochester, NY 14642, USA. robert_block@urmc.rochester.edu
authors.forename: Robert C
authors.initials: RC
authors.lastname: Block
authors.forename: Lisa
authors.initials: L
authors.lastname: Kakinami
authors.forename: Matthew
authors.initials: M
authors.lastname: Jonovich
authors.forename: Illena
authors.initials: I
authors.lastname: Antonetti
authors.forename: Peter
authors.initials: P
authors.lastname: Lawrence
authors.forename: Nida
authors.initials: N
authors.lastname: Meednu
authors.forename: Pedro
authors.initials: P
authors.lastname: CalderonArtero
authors.forename: Shaker A
authors.initials: SA
authors.lastname: Mousa
authors.forename: J Thomas
authors.initials: JT
authors.lastname: Brenna
authors.forename: Steve
authors.initials: S
authors.lastname: Georas
mesh_terms: D001241:Aspirin / Q000008:administration & dosage / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D004338:Drug Combinations; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D010979:Platelet Function Tests
chemical_list: D004338:Drug Combinations; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; D001241:Aspirin