title: Derivatives of the cationic plant alkaloids berberine and palmatine amplify protonophorous activity of fatty acids in model membranes and mitochondria.
abstract: Previously it has been shown by our group that berberine and palmatine, penetrating cations of plant origin, when conjugated with plastoquinone (SkQBerb and SkQPalm), can accumulate in isolated mitochondria or in mitochondria of living cells and effectively protect them from oxidative damage. In the present work, we demonstrate that SkQBerb, SkQPalm, and their analogs lacking the plastoquinone moiety (C10Berb and C10Palm) operate as mitochondria-targeted compounds facilitating protonophorous effect of free fatty acids. These compounds induce proton transport mediated by small concentrations of added fatty acids both in planar and liposomal model lipid membranes. In mitochondria, such an effect can be carried out by endogenous fatty acids and the adenine nucleotide translocase.
authors.affiliation: Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, Vorobyevy Gory 1, 119991 Moscow, Russia.
authors.forename: Antonina V
authors.initials: AV
authors.lastname: Pustovidko
authors.forename: Tatiana I
authors.initials: TI
authors.lastname: Rokitskaya
authors.forename: Inna I
authors.initials: II
authors.lastname: Severina
authors.forename: Ruben A
authors.initials: RA
authors.lastname: Simonyan
authors.forename: Tatiana A
authors.initials: TA
authors.lastname: Trendeleva
authors.forename: Konstantin G
authors.initials: KG
authors.lastname: Lyamzaev
authors.forename: Yuri N
authors.initials: YN
authors.lastname: Antonenko
authors.forename: Anton G
authors.initials: AG
authors.lastname: Rogov
authors.forename: Renata A
authors.initials: RA
authors.lastname: Zvyagilskaya
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Skulachev
authors.forename: Boris V
authors.initials: BV
authors.lastname: Chernyak
mesh_terms: D000470:Alkaloids / Q000378:metabolism; D001599:Berberine / Q000378:metabolism*; D001600:Berberine Alkaloids / Q000378:metabolism*; D002412:Cations / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D008566:Membranes / Q000187:drug effects / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D010971:Plastoquinone / Q000378:metabolism
chemical_list: D000470:Alkaloids; D001600:Berberine Alkaloids; D002412:Cations; D005227:Fatty Acids; D001599:Berberine; D006859:Hydrogen; C005413:palmatine; D010971:Plastoquinone