title: Short-coherence off-axis holographic phase microscopy of live cell dynamics.
abstract: We demonstrate a single-shot holographic phase microscope that combines short-coherence laser pulses with an off-axis geometry. By introducing a controlled pulse front tilt, ultrashort pulses are made to interfere over a large field-of-view without loss of fringe contrast. With this microscope, quantitative phase images of live cells can be recorded in a full-field geometry without moving parts. We perform phase imaging of HEK293 cells, to study the dynamics of cell volume regulation in response to an osmotic shock.
authors.affiliation: These authors contributed equally to this work ; LaserLaB, VU University Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam,  The Netherlands.
authors.forename: Stefan
authors.initials: S
authors.lastname: Witte
authors.forename: Andrius
authors.initials: A
authors.lastname: Plauşka
authors.forename: Margreet C
authors.initials: MC
authors.lastname: Ridder
authors.forename: Laura
authors.initials: L
authors.lastname: van Berge
authors.forename: Huibert D
authors.initials: HD
authors.lastname: Mansvelder
authors.forename: Marie Louise
authors.initials: ML
authors.lastname: Groot