title: Pharmacokinetics and modeling of immune cell trafficking: quantifying differential influences of target tissues versus lymphocytes in SJL and lipopolysaccharide-treated mice.
abstract: BACKGROUND
  Immune cell trafficking into the CNS and other tissues plays important roles in health and disease. Rapid quantitative methods are not available that could be used to study many of the dynamic aspects of immune cell-tissue interactions.
  
  
  METHODS
  We used pharmacokinetics and modeling to quantify and characterize the trafficking of radioactively labeled lymphocytes into brain and peripheral tissues. We used variance from two-way ANOVAs with 2 × 2 experimental designs to model the relative influences of lymphocytes and target tissues in trafficking.
  
  
  RESULTS
  We found that in male CD-1 mice, about 1 in 5,000 intravenously injected lymphocytes entered each gram of brain. Uptake by brain was 2 to 3 times higher in naïve SJL females, but uptake by spleen and clearance from blood was lower, demonstrating a dichotomy in immune cell distribution. Treatment of CD-1 mice with lipopolysaccharide (LPS) increased immune cell uptake into brain but decreased uptake by spleen and axillary nodes.
  
  
  CONCLUSIONS
  Differences in brain uptake and in uptake by spleen between SJL and CD-1 mice were primarily determined by lymphocytes, whereas differences in uptake with LPS were primarily determined by lymphocytes for the brain but by the tissues for the spleen and the axillary lymph node. These results show that immune cells normally enter the CNS and that tissues and immune cells interact in ways that can be quantified by pharmacokinetic models.
authors.affiliation: GRECC, Veterans Affairs Puget Sound Health Care System, Seattle, WA, USA. wabanks1@uw.edu
authors.forename: William A
authors.initials: WA
authors.lastname: Banks
authors.forename: Michael L
authors.initials: ML
authors.lastname: Niehoff
authors.forename: Nicholas M
authors.initials: NM
authors.lastname: Ponzio
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Erickson
authors.forename: Steven S
authors.initials: SS
authors.lastname: Zalcman
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D005260:Female; D054879:Halogenation / Q000502:physiology; D007456:Iodine Isotopes / Q000493:pharmacokinetics; D017493:Leukocyte Common Antigens; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D013997:Time Factors
chemical_list: D007456:Iodine Isotopes; D008070:Lipopolysaccharides; D017493:Leukocyte Common Antigens