title: [Influence of anti-ICOS antibody on quantity and function of CD4(+);CD25(+);Foxp3(+);Treg from lymph and blood of rats with bronchial asthma].
abstract: OBJECTIVE
  To investigate the influences of anti-ICOS antibody (anti-ICOSAb) on quantity and function of CD4(+);CD25(+);Foxp3(+);Treg cells from lymph and peripheral blood of rats with bronchial asthma.
  
  
  METHODS
  The mononuclear cells (MNC) from lymph and blood were co-cultured with anti-ICOSAb,  and then the percentage of CD4(+);CD25(+);Foxp3(+);Treg cells were analyzed by flow cytometer (FCM) and the levels of IL-10 and TGF-β1 in supernatants were determined by ELISA.
  
  
  RESULTS
  The MNC were collected from lymph and blood at 0,  24 and 48 h after the last challenge,  respectively,  and the cells were cultured for 96 h in vitro. The percentage of CD4(+);CD25(+);Foxp3(+);Treg cells in the MNC from lymph was significantly higher than that from blood in each group (P<0.05);   The percentage of CD4(+);CD25(+);Foxp3(+);Treg cells in the MNC from lymph and blood in asthma group was significantly lower compare with the normal control group (P<0.05);   The percentage of CD4(+);CD25(+);Foxp3(+);Treg cells in the MNC from lymph and blood in the anti-ICOSAb group obviously decreased compare with the asthma group (P<0.05). At 0 h after the last challenge,  the level of IL-10 in the supernatant of MNC from lymph and blood in the anti-ICOSAb group were significantly lower than that of the control and asthma groups (P<0.05),  while there were no significant differences of TGF-β1 expression in the supernatant of MNC from lymph and blood in each group at different time points.
  
  
  CONCLUSIONS
  Blocking the ICOS/ICOSL signaling pathway by anti-ICOSAb could exacerbate the deficiency of CD4(+);CD25(+);Foxp3(+);Treg cells from lymph and blood in bronchial asthmatic rat,  meanwhile inhibit the CD4(+);CD25(+);Foxp3(+);Treg cells secreting IL-10 at 0 h after the last challenge,  but have no significant effect on the secretion of TGF-β1.
authors.affiliation: Department of Pediatrics, Binzhou Medical University, Binzhou, China. zhaoleinmg@163.com
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Xue-bin
authors.initials: XB
authors.lastname: Feng
authors.forename: Yue-si
authors.initials: YS
authors.lastname: Wang
authors.forename: Qing-qing
authors.initials: QQ
authors.lastname: Cui
authors.forename: Yin-yin
authors.initials: YY
authors.lastname: Cao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology*; D015703:Antigens, CD / Q000378:metabolism; D001249:Asthma / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D018791:CD4 Lymphocyte Count; D004195:Disease Models, Animal; D005260:Female; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology*; D016130:Immunophenotyping; D060889:Inducible T-Cell Co-Stimulator Protein / Q000276:immunology*; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D007155:Immunologic Factors; D060889:Inducible T-Cell Co-Stimulator Protein; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10