title: Genome-wide assessment in Escherichia coli reveals time-dependent nanotoxicity paradigms.
abstract: The use of engineered nanomaterials (eNM) in consumer and industrial products is increasing exponentially. Our ability to rapidly assess their potential effects on human and environmental health is limited by our understanding of nanomediated toxicity. High-throughput screening (HTS) enables the investigation of nanomediated toxicity on a genome-wide level, thus uncovering their novel mechanisms and paradigms. Herein, we investigate the toxicity of zinc-containing nanomaterials (Zn-eNMs) using a time-resolved HTS methodology in an arrayed  Escherichia coli  genome-wide knockout (KO) library. The library was screened against nanoscale zerovalent zinc (nZn), nanoscale zinc oxide (nZnO), and zinc chloride (ZnCl(2)) salt as reference. Through sequential screening over 24 h, our method identified 173 sensitive clones from diverse biological pathways, which fell into two general groups: early and late responders. The overlap between these groups was small. Our results suggest that bacterial toxicity mechanisms change from pathways related to general metabolic function, transport, signaling, and metal ion homeostasis to membrane synthesis pathways over time. While all zinc sources shared pathways relating to membrane damage and metal ion homeostasis, Zn-eNMs and ZnCl(2) displayed differences in their sensitivity profiles. For example, ZnCl(2) and nZnO elicited unique responses in pathways related to two-component signaling and monosaccharide biosynthesis, respectively. Single isolated measurements, such as MIC or IC(50), are inadequate, and time-resolved approaches utilizing genome-wide assays are therefore needed to capture this crucial dimension and illuminate the dynamic interplay at the nano-bio interface.
authors.affiliation: Department of Civil & Environmental Engineering, University of California, Los Angeles, California 90095, United States.
authors.forename: Vincent C
authors.initials: VC
authors.lastname: Reyes
authors.forename: Minghua
authors.initials: M
authors.lastname: Li
authors.forename: Eric M V
authors.initials: EM
authors.lastname: Hoek
authors.forename: Shaily
authors.initials: S
authors.lastname: Mahendra
authors.forename: Robert
authors.initials: R
authors.lastname: Damoiseaux
mesh_terms: D000465:Algorithms; D001681:Biological Assay / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D016680:Genome, Bacterial / Q000187:drug effects* / Q000235:genetics*; D009152:Mutagenicity Tests / Q000379:methods*; D053758:Nanoparticles / Q000633:toxicity*