title: Quantitative mass spectrometry for colorectal cancer proteomics.
abstract: This review documents the uses of quantitative MS applied to colorectal cancer (CRC) proteomics for biomarker discovery and molecular pathway profiling. Investigators are adopting various labeling and label-free MS approaches to quantitate differential protein levels in cells, tumors, and plasma/serum. We comprehensively review recent uses of this technology to examine mouse models of CRC, CRC cell lines, their secretomes and subcellular fractions, CRC tumors, CRC patient plasma/serum, and stool samples. For biomarker discovery these approaches are uncovering proteins with potential diagnostic and prognostic utility, while in vitro cell culture experiments are characterizing proteomic and phosphoproteomic responses to disrupted signaling pathways due to mutations or to inhibition of drugable enzymes.
authors.affiliation: Australian Proteome Analysis Facility (APAF), Department of Chemistry & Biomolecular Sciences, Macquarie University, Sydney, Australia.
authors.forename: Juan
authors.initials: J
authors.lastname: Martínez-Aguilar
authors.forename: Jenny
authors.initials: J
authors.lastname: Chik
authors.forename: Judith
authors.initials: J
authors.lastname: Nicholson
authors.forename: Crystal
authors.initials: C
authors.lastname: Semaan
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: McKay
authors.forename: Mark P
authors.initials: MP
authors.lastname: Molloy
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000628:therapy; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor