title: Differentiated hamster sebocytes exhibit apoptosis-resistant phenotype by the augmentation of intracellular calcium level in vitro.
abstract: Sebaceous glands play important roles in the maintenance of the skin barrier function by secreting sebum onto the skin surface. In our study, we demonstrated that differentiated hamster sebocytes (DHS) exhibited apoptosis resistance and the loss of Ca(2+)  influx against a calcium ionophore, A23187 treatment, which induced both apoptosis and Ca(2+)  influx in undifferentiated hamster sebocytes (unDHS). The Fluo-3-related signal of intracellular Ca(2+)  in the DHS was higher than that in unDHS and was sustained even though there was a depletion of Ca(2+)  from the culture medium. Furthermore, the intracellular Ca(2+)  chelator, BAPTA-AM, was found to decrease the Ca(2+)  signal in the DHS, which induced apoptosis. Thus, these results provide novel evidence that the cell differentiation-dependent increase in store-operated Ca(2+)  release is associated with apoptosis resistance in the DHS. Moreover, these findings should accelerate the understanding of the mechanisms of sebogenesis and/or sebum production and secretion under physiological conditions.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kurihara
authors.forename: Takashi
authors.initials: T
authors.lastname: Sato
authors.forename: Noriko
authors.initials: N
authors.lastname: Akimoto
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D061207:Calcium Ionophores / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D006224:Cricetinae; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D012627:Sebaceous Glands / Q000187:drug effects / Q000378:metabolism*; D012629:Sebum / Q000378:metabolism
chemical_list: D061207:Calcium Ionophores; D002614:Chelating Agents; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D000001:Calcimycin; D004533:Egtazic Acid; D002118:Calcium