title: Structural investigation of water-soluble polysaccharides extracted from the fruit bodies of Coprinus comatus.
abstract: Water-soluble polysaccharide material, extracted from the stipes of the fruit bodies of Coprinus comatus by hot water, was fractionated by sequential weak anion-exchange and size-exclusion chromatography. The relevant fractions were subjected to structural analysis, including (d/l) monosaccharide/methylation analysis and 1D/2D NMR spectroscopy. Besides the disaccharide α,α-trehalose [α-D-Glcp-(1↔1)-α-D-Glcp], high-molecular-mass α-D-glucans (the most abundant component) consisting of [→4)-α-D-Glcp-(1→](n) backbones with ~10% branching at C-6 by terminal α-D-Glcp-(1→6)- or α-D-Glcp-(1→6)-α-D-Glcp-(1→6)- units, lower-molecular-mass linear β-D-glucans consisting of [→6)-β-D-Glcp-(1→](m) sequences, and a lower-molecular-mass pentasaccharide-repeating α-L-fuco-α-D-galactan, {→6)-α-D-Galp-(1→6)-[α-L-Fucp-(1→2)-]α-D-Galp-(1→6)-α-D-Galp-(1→6)-α-D-Galp-(1→}(p), were found to be present.
authors.affiliation: School of Food Science, Henan Institute of Science and Technology, Hualan Road, Xinxiang 453003, PR China. libowuxi@yahoo.com.cn
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Justyna M
authors.initials: JM
authors.lastname: Dobruchowska
authors.forename: Gerrit J
authors.initials: GJ
authors.lastname: Gerwig
authors.forename: Lubbert
authors.initials: L
authors.lastname: Dijkhuizen
authors.forename: Johannis P
authors.initials: JP
authors.lastname: Kamerling
mesh_terms: D002240:Carbohydrate Sequence; D003302:Coprinus / Q000737:chemistry*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D014867:Water