title: [Optimization on ultrasonic extraction technology of total coumarines in cortex fraxini].
abstract: OBJECTIVE
  To optimize the ultrasonic extraction technology of total coumarins in Cortex Fraxini.
  
  
  METHODS
  The effects of ethanol concentration, ratio of solid to liquid, extraction temperature, ultrasonic frequency and extraction time in extraction procedure on the extraction rate of total coumarins in Cortex Fraxini was researched by single factor experiment, the optimal extraction technology was determined by orthogonal experiment.
  
  
  RESULTS
  The optimal extraction technology of total coumarins in Cortex Fraxini was:mixing the material with 60% ethanol according to ratio of solid to liquid 1:10 (W/V), ultrasonic frequency 175 W and extracting 40 min at 50 degrees C. The extraction rate of total coumarins reached 6.283% with the optimal extraction technology.
  
  
  CONCLUSIONS
  Compares with the classic Soxhlet extraction, ultrasound is a less time-comsuming, low energy and great efficiency tool for the fast extraction of coumarins from Cortex Fraxini.
authors.affiliation: Key Laboratory of Plant Resources Conservation and Utilization, Jishou University, Jishou 416000, China.
authors.forename: Li-Zhi
authors.initials: LZ
authors.lastname: Xiong
authors.forename: Qing
authors.initials: Q
authors.lastname: Ma
authors.forename: Hua
authors.initials: H
authors.lastname: Long
mesh_terms: D031319:Aesculus; D003374:Coumarins / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D029592:Oleaceae / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; D012997:Solvents; C517354:horse chestnut seed; D000431:Ethanol