title: Assays for DNA damage.
abstract: This unit describes several assays for detecting several kinds of DNA damage (strand breaks, internal crosslinking, DNA/protein crosslinks) and repair activity following exposure to genotoxic agents. The methods include single-cell electrophoresis (comet assay), filter eluting, K-SDS precipitation, and measurement of unscheduled DNA synthesis.
authors.affiliation: New York University School of Medicine, New York, New York, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Sutherland
authors.forename: M
authors.initials: M
authors.lastname: Costa
mesh_terms: D002478:Cells, Cultured; D020552:Comet Assay / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009152:Mutagenicity Tests; D009153:Mutagens / Q000145:classification / Q000633:toxicity*; D015262:Xenobiotics / Q000145:classification / Q000633:toxicity*
chemical_list: D009153:Mutagens; D015262:Xenobiotics; D004247:DNA