title: A novel fluorescent sensor for mutational p53 DNA sequence detection based on click chemistry.
abstract: A novel fluorescent sensor for DNA sequence has been designed by taking advantages of copper nanoparticles (CuNPs) selectively formed on double stranded (ds) DNA template and Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. Copper(II) is derived from CuNPs which previously formed on the dsDNA template, and then copper(II) is reduced to copper(I) by ascorbate, which in turn induced CuAAC reaction between the weak-fluorescent compound (3-azido-7-hydroxycoumarin) and propargyl alcohol to form strong fluorescence compounds (1,2,3-triazole compounds). Since CuNPs are accumulated efficiently in the major groove of dsDNA and ssDNA has no groove, it indicates that the proposed sensor owns the merits of low detection limit, high sensitivity and selectivity for mutational p53 sequence detection. Additionally, the method has been successfully applied to recognize the sequence which contains a single-base mismatch in the short human p53 gene fragment. Furthermore, it has also been applied to detect DNA sequence in complex medium (hela cellular homogenate) with satisfactory results.
authors.affiliation: MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, 523 Gongye Road, Fuzhou, Fujian 350002, China.
authors.forename: Suyan
authors.initials: S
authors.lastname: Qiu
authors.forename: Xianghui
authors.initials: X
authors.lastname: Li
authors.forename: Wenming
authors.initials: W
authors.lastname: Xiong
authors.forename: Lidan
authors.initials: L
authors.lastname: Xie
authors.forename: Longhua
authors.initials: L
authors.lastname: Guo
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation*; D057930:Click Chemistry / Q000295:instrumentation*; D004247:DNA / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53; D004247:DNA