title: Turn-on fluorescence sensor based on single-walled-carbon-nanohorn-peptide complex for the detection of thrombin.
abstract: Proteases play a central role in several widespread diseases. Thus, there is a great need for the fast and sensitive detection of various proteolytic enzymes. Herein, we have developed a carbon nanotube (CNT)-based protease biosensing platform that uses peptides as a fluorescence probe for the first time. Single-walled carbon nanohorns (SWCNHs) and thrombin were used to demonstrate this detection strategy. SWCNHs can adsorb a fluorescein-based dye (FAM)-labeled peptide (FAM-pep) and quench the fluorescence of FAM. In contrast, thrombin can cleave FAM-pep on SWCNHs and recover the fluorescence of FAM, which allows the sensitive detection of thrombin. This biosensor has a high sensitivity and selectivity toward thrombin, with a detection limit of 100 pM.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences Changchun, Jilin 130022, PR China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhu
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Liu
authors.forename: Lianzhe
authors.initials: L
authors.lastname: Hu
authors.forename: Yali
authors.initials: Y
authors.lastname: Yuan
authors.forename: Guobao
authors.initials: G
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques; D005453:Fluorescence; D057230:Limit of Detection; D036103:Nanotechnology; D037742:Nanotubes, Carbon; D010455:Peptides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry*
chemical_list: D037742:Nanotubes, Carbon; D010455:Peptides; D013917:Thrombin