title: Increased recovery and improved purity of PHA from recombinant Cupriavidus necator.
abstract: A simple procedure for recovering biodegradable polymer from bacterial cells has been developed using economical and environmentally friendly solvent or chemicals. Recombinant bacterium, Cupriavidus necator harboring pBBR1MCS-C2 plasmid polyhydroxyalkanoate (PHA) synthase gene was used for the production of copolymer P(3HB-co-3HHx) from crude palm kernel oil (CPKO). NaOH was chosen in this study as it could give high purity and recovery yield. Increase of NaOH concentration had resulted in an increase of the PHA purity, but the recovery yield had decreased. The greater improvement of PHA purity and recovery were achieved by incubating the freeze-dried cells (10-30 g/L) in NaOH (0.1 M) for 1-3 h at 30°C and polishing using 20% (v/v) of ethanol. The treatment caused negligible degradation of the molecular weight of PHA recovered from the bacterial cells. The present review also highlights other extraction methods to provide greater insights into economical and sustainable recovery of PHA from bacterial cells.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia.
authors.forename: Siti Nor Syairah
authors.initials: SN
authors.lastname: Anis
authors.forename: Nurhezreen Md
authors.initials: NM
authors.lastname: Iqbal
authors.forename: Sudesh
authors.initials: S
authors.lastname: Kumar
authors.forename: Amirul
authors.initials: A
authors.lastname: Al-Ashraf
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D054813:Polyhydroxyalkanoates / Q000378:metabolism*
chemical_list: D054813:Polyhydroxyalkanoates; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase