title: Impact of different dimethyl sulphoxide concentrations on cell recovery, viability and clonogenic potential of cryopreserved peripheral blood hematopoietic stem and progenitor cells.
abstract: OBJECTIVE
  The procedure of autologous hematopoietic stem/progenitor cell transplantation requires cryopreservation. Addition of DMSO is necessary to secure the viability of such cells, but this solvent is potentially toxic to stem cells' recipient. 10% DMSO solution is used by the majority of transplant centres. The aim of our study was to test if DMSO concentration might be reduced without negative impact on cell recovery and clonogenicity.
  
  
  METHODS
  Samples were prospectively collected from 20 patients. Small volumes of leukapheresis products were frozen with different cryoprotective mixtures, containing 10%, 7·5%, 5% and 2·5% DMSO, respectively. The quality of cryoprotective mixtures was evaluated based on recovery, viability and clonogenic potential of hematopoietic stem cells after defreezing.
  
  
  RESULTS
  Reduction in DMSO concentration to 7·5% or lower was associated with decreased recovery of nucleated cells. In contrast, the number of colonies was highest for 7·5% DMSO with significant differences when compared to 10% DMSO solution.
  
  
  CONCLUSIONS
  Reduction in DMSO concentration from 10% to 7·5% may have favourable impact on hematopoietic recovery after autologous transplantation. The findings require confirmation in a clinical setting.
authors.affiliation: Department of Bone Marrow Transplantation, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland. asmagur@io.gliwice.pl
authors.forename: A
authors.initials: A
authors.lastname: Smagur
authors.forename: I
authors.initials: I
authors.lastname: Mitrus
authors.forename: S
authors.initials: S
authors.lastname: Giebel
authors.forename: M
authors.initials: M
authors.lastname: Sadus-Wojciechowska
authors.forename: J
authors.initials: J
authors.lastname: Najda
authors.forename: T
authors.initials: T
authors.lastname: Kruzel
authors.forename: T
authors.initials: T
authors.lastname: Czerw
authors.forename: J
authors.initials: J
authors.lastname: Gliwinska
authors.forename: M
authors.initials: M
authors.lastname: Prokop
authors.forename: M
authors.initials: M
authors.lastname: Glowala-Kosinska
authors.forename: A
authors.initials: A
authors.lastname: Chwieduk
authors.forename: J
authors.initials: J
authors.lastname: Holowiecki
mesh_terms: D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000008:administration & dosage; D004121:Dimethyl Sulfoxide / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007937:Leukapheresis / Q000379:methods; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D013234:Stem Cells / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology
chemical_list: D018952:Antigens, CD34; D003451:Cryoprotective Agents; D016179:Granulocyte Colony-Stimulating Factor; D004121:Dimethyl Sulfoxide