title: Expression of DNA methyltransferase genes in four-cell bovine embryos cultured in the presence of oviductal fluid.
abstract: The possible epigenomic effect of oviductal fluid on expression of DNA methyltransferase (DNMT) genes was examined in early bovine embryos (4-cell stage). Real-time quantitative PCR was performed to determine the relative expression of DNMT1, DNMT3a and DNMT3b transcripts in embryos cultured in vitro in the presence or absence of oviductal fluid. Expression of DNMT1 significantly increased when cultured with oviductal fluid, whereas DNMT3a and DNMT3b transcripts were unaffected by the addition of oviductal fluid. These results may help reveal the role of oviductal factors in the regulation of DNMT expression.
authors.affiliation: Instituto Superior de Investigaciones Biológicas (INSIBIO), Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina.
authors.forename: D
authors.initials: D
authors.lastname: Barrera
authors.forename: E V
authors.initials: EV
authors.lastname: García
authors.forename: F
authors.initials: F
authors.lastname: Sinowatz
authors.forename: G A
authors.initials: GA
authors.lastname: Palma
authors.forename: M A
authors.initials: MA
authors.lastname: Jiménez-Díaz
authors.forename: D C
authors.initials: DC
authors.lastname: Miceli
mesh_terms: D000818:Animals; D002417:Cattle / Q000196:embryology*; D003470:Culture Media / Q000737:chemistry*; D015254:DNA Modification Methylases / Q000235:genetics / Q000378:metabolism*; D046149:Embryo Culture Techniques / Q000662:veterinary*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D012313:RNA / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; D012313:RNA; D015254:DNA Modification Methylases