title: European ring trial to evaluate ELISAs for the diagnosis of infection with Rift Valley fever virus.
abstract: A ring trial was organized to evaluate Rift Valley fever virus (RVFV) ELISAs by European laboratories. A total of five ELISAs, two of which specific for IgM antibodies, were evaluated by six participants. Sera were derived from cattle or sheep and originated from either a RVFV endemic area, a RVFV-free area or from experimental infection studies. Cohen's kappa analysis showed higher than 90% agreement of two commercially available ELISAs with the virus neutralization test, suggesting that primary screening as well as serological confirmation using these ELISAs is feasible. More extensive validations with sera of known IgM status are, however, required to determine agreement between IgM ELISAs.
authors.affiliation: Department of Virology, Central Veterinary Institute of Wageningen University and Research Centre, Lelystad, The Netherlands. jeroen.kortekaas@wur.nl
authors.forename: Jeroen
authors.initials: J
authors.lastname: Kortekaas
authors.forename: Jet
authors.initials: J
authors.lastname: Kant
authors.forename: Rianka
authors.initials: R
authors.lastname: Vloet
authors.forename: Catherine
authors.initials: C
authors.lastname: Cêtre-Sossah
authors.forename: Philippe
authors.initials: P
authors.lastname: Marianneau
authors.forename: Sandra
authors.initials: S
authors.lastname: Lacote
authors.forename: Ashley C
authors.initials: AC
authors.lastname: Banyard
authors.forename: Claire
authors.initials: C
authors.lastname: Jeffries
authors.forename: Martin
authors.initials: M
authors.lastname: Eiden
authors.forename: Martin
authors.initials: M
authors.lastname: Groschup
authors.forename: Susanne
authors.initials: S
authors.lastname: Jäckel
authors.forename: Esther
authors.initials: E
authors.lastname: Hevia
authors.forename: Alejandro
authors.initials: A
authors.lastname: Brun
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005060:Europe; D007075:Immunoglobulin M / Q000097:blood*; D009500:Neutralization Tests; D012295:Rift Valley Fever / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D012296:Rift Valley fever virus / Q000276:immunology*; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M