title: Phosphorylation of measles virus phosphoprotein at S86 and/or S151 downregulates viral transcriptional activity.
abstract: Measles virus phosphoprotein (P protein) is a cofactor of the viral RNA polymerase (L protein) that associates with the nucleoprotein-RNA complex to support viral transcription and replication. Here, we report a significant inverse correlation between the phosphorylation level of MV-P protein and viral transcriptional activity. Upregulation of P protein phosphorylation resulted in reduction of viral transcription. Additionally, we found that strong phosphorylation at S86 and S151 of P protein, which may be generally prevented by association with nucleoprotein, downregulates the viral transcriptional activity. These findings suggest that P protein is involved in regulation of viral transcription through changes in its phosphorylation status.
authors.affiliation: International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Sugai
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sato
authors.forename: Misako
authors.initials: M
authors.lastname: Yoneda
authors.forename: Chieko
authors.initials: C
authors.lastname: Kai
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003067:Coenzymes / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D057809:HEK293 Cells; D006801:Humans; D008459:Measles virus / Q000201:enzymology / Q000235:genetics*; D009698:Nucleoproteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics; D012694:Serine / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: D003067:Coenzymes; D009698:Nucleoproteins; C055859:P protein, Sendai virus; D010750:Phosphoproteins; D012367:RNA, Viral; D014764:Viral Proteins; D012694:Serine