title: Effect of ischemic preconditioning on injuries caused by ischemia and reperfusion in rat intestine.
abstract: To study whether ischemic preconditioning (IPC) attenuated intestinal dysfunction caused by ischemia (I) and reperfusion (R), rats were underwent 60 minutes of I which was produced by occlusion of the superior mesenteric artery, and/or 120 minutes R. The IPC group had the I procedure previously stimulated for 5 minutes and the R for 10 minutes. IPC and sham groups were injected with saline solution (SS) via the femoral vein 5 minutes before the I and R, and for R. After I or I/R, 2-cm jejunal segments were mounted in an organ bath to study neurogenic contractions stimulated by electrical pulses or KCl using a digital recording system. Thin jejunal slices were stained with hematoxylin and eosin for optical microscopy. Compared with the sham group, jejunal contractions were similar in the IPC + I and the IPC + I/R groups, but reduced in the I + SS and the I/R + SS groups. The jejunal enteric nerves were damaged in the I + SS and the I/R + SS groups, but not in the IPC groups. These results suggested that ischemic preconditioning attenuated intestinal dysfunction caused by I and I/R.
authors.affiliation: Department of Pharmacology, Federal University of São Paulo (UNIFESP), São Paulo, Brazil. taha@uol.com.br
authors.forename: M O
authors.initials: MO
authors.lastname: Taha
authors.forename: R
authors.initials: R
authors.lastname: Miranda-Ferreira
authors.forename: A C R
authors.initials: AC
authors.lastname: Chang
authors.forename: A M
authors.initials: AM
authors.lastname: Rodrigues
authors.forename: I S
authors.initials: IS
authors.lastname: Fonseca
authors.forename: L B
authors.initials: LB
authors.lastname: Toral
authors.forename: M R
authors.initials: MR
authors.lastname: Cardoso
authors.forename: M J
authors.initials: MJ
authors.lastname: Simões
authors.forename: I S
authors.initials: IS
authors.lastname: Oliveira-Junior
authors.forename: H P
authors.initials: HP
authors.lastname: Monteiro
authors.forename: D J
authors.initials: DJ
authors.lastname: Fagundes
authors.forename: N S A
authors.initials: NS
authors.lastname: Taha
authors.forename: A
authors.initials: A
authors.lastname: Caricati-Neto
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004558:Electric Stimulation; D017615:Enteric Nervous System / Q000503:physiopathology; D005769:Gastrointestinal Motility; D019194:Ischemic Preconditioning; D007583:Jejunum / Q000098:blood supply* / Q000187:drug effects / Q000294:innervation / Q000473:pathology / Q000503:physiopathology; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*
chemical_list: D011189:Potassium Chloride