title: Gene delivery of PEI incorporating with functional block copolymer via non-covalent assembly strategy.
abstract: A novel functional diblock polymer P(PEGMA-b-MAH) is prepared and incorporated to improve the gene delivery efficiency of poly(ethyleneimine) PEI via non-covalent assembly strategy. First, P(PEGMA-b-MAH) is prepared from l-methacrylamidohistidine methyl ester (MAH) by reversible addition fragmentation chain transfer polymerization, with poly[poly(ethylene glycol) methyl ether methacrylate] (P(PEGMA)) as the macroinitiator. Then P(PEGMA-b-MAH) is assembled with plasmid DNA (pDNA) and PEI (M(w)=10kDa) to form PEI/P(PEGMA-b-MAH)/pDNA ternary complexes. The agarose gel retardation assay shows that the presence of P(PEGMA-b-MAH) does not interfere with DNA condensation by the PEI. Dynamic light scattering tests show that PEI/P(PEGMA-b-MAH)/pDNA ternary complexes have excellent serum stability. In vitro transfection indicates that, compared to the P(PEGMA-b-MAH) free PEI-25k/pDNA binary complexes, PEI-10k/P(PEGMA-b-MAH)/pDNA ternary complexes have lower cytotoxicity and higher gene transfection efficiency, especially under serum conditions. The ternary complexes proposed here can inspire a new strategy for the development of gene and drug delivery vectors.
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Weijin Road No. 94, Tianjin 300071, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Hu
authors.forename: Dezhong
authors.initials: D
authors.lastname: Zhou
authors.forename: Congxin
authors.initials: C
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.forename: Jiatong
authors.initials: J
authors.lastname: Chen
authors.forename: Zhengpu
authors.initials: Z
authors.lastname: Zhang
authors.forename: Tianying
authors.initials: T
authors.lastname: Guo
mesh_terms: D002021:Buffers; D016923:Cell Death; D002470:Cell Survival; D004247:DNA / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D018014:Gene Transfer Techniques; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D055672:Static Electricity; D017186:Titrimetry; D014162:Transfection
chemical_list: D002021:Buffers; D008689:Methacrylates; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D004247:DNA