title: Der p 2 recombinant bacille Calmette-Guérin targets dendritic cells to inhibit allergic airway inflammation in a mouse model of asthma.
abstract: BACKGROUND
  Previous studies showed that a recombinant bacille Calmette-Guérin (rBCG) which expressed the Der p 2 of house dust mites (Der p 2 rBCG) could suppress asthmatic airway inﬂammation. There are two possible mechanisms: (1) Der p 2 rBCG elicits immune deviation from Th2 to Th1, and (2) Der p 2 rBCG induces antigen-specific regulatory T cells. However, the role of dendritic cell (DC) Der p 2 rBCG in this protective effect and in reprogramming T-cell commitment still needs to be studied.
  
  
  OBJECTIVE
  The aim of this study was to determine whether DCs play a central role in the Der p 2 rBCG-mediated inhibition of allergic airway inflammation.
  
  
  METHODS
  DCs were collected from Der p 2 rBCG-immunized mice (Der p 2 rBCG-DCs) and adoptively transferred to Der p 2-sensitized mice. The effects of DCs on airway inflammation and immune regulation were analyzed.
  
  
  RESULTS
  Adoptive transfer of DCs from Der p 2 rBCG-immunized mice suppressed asthmatic responses, including airway inﬂammation, mucin secretion and airway responsiveness. Der p 2 rBCG-DCs could effectively inhibit excessive Th2 immune responses and induced a subtype of CD4+CD25+Foxp3+ anti-specific regulatory T cells in this asthma model. Furthermore, Der p 2 rBCG immunization recruited more plasmacytoid DCs in abdominal draining lymph nodes.
  
  
  CONCLUSIONS
  These findings suggest that DCs played a key role in Der p 2 rBCG-induced immunoregulation. Compared with BCG, Der p 2 rBCG displayed a more potent inhibitory effect on asthma responses, which may be related to the increase in plasmacytoid DC recruitment. These results improve our understanding of the cellular basis of Der p 2 BCG-mediated inhibition of asthma.
authors.affiliation: Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Shuo-Yao
authors.initials: SY
authors.lastname: Qu
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Ou-Yang
authors.forename: Ya-Long
authors.initials: YL
authors.lastname: He
authors.forename: Qi
authors.initials: Q
authors.lastname: Wan
authors.forename: Jie-Ran
authors.initials: JR
authors.lastname: Shi
authors.forename: Chang-Gui
authors.initials: CG
authors.lastname: Wu
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology / Q000627:therapeutic use*; D060829:Arthropod Proteins / Q000276:immunology / Q000627:therapeutic use*; D001249:Asthma / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D001500:BCG Vaccine / Q000276:immunology / Q000627:therapeutic use*; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007249:Inflammation / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000627:therapeutic use; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D001500:BCG Vaccine; C451163:Dermatophagoides pteronyssinus antigen p 2; D011993:Recombinant Fusion Proteins