title: Measurement of erythrocyte protoporphyrin concentration by double extraction and spectrofluorometry.
abstract: Quantification of the level of erythrocyte protoporphyrin can be used as a screening assay for lead exposure. In this method, porphyrins and heme compounds are extracted from whole blood using ethyl acetate, and porphyrins are then separated from heme by back-extraction with HCl solution. The extracted porphyrins are then quantified using a spectrofluorometer calibrated with protoporphyrin IX standard solutions.
authors.affiliation: Wadsworth Center, New York State Department of Health, Albany, New York, USA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Parsons
mesh_terms: D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011524:Protoporphyrins / Q000097:blood* / Q000302:isolation & purification; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
chemical_list: D011524:Protoporphyrins; D012997:Solvents; C017803:zinc protoporphyrin