title: Hippocampal heat shock protein 25 expression in streptozotocin-induced diabetic mice.
abstract: Hippocampal abnormalities are believed to increase the risk of cognitive decline in diabetic patients. The underlying mechanism is unknown, but both hyperglycemia and oxidative stress have been implicated. Cellular stresses induce the expression of heat shock protein 25 (HSP25) and this results in cytoprotection. Our aim was to assess hippocampal expression of HSP25 in experimental diabetes. Mice were rendered diabetic by streptozotocin injection. Ten weeks after diabetes onset hippocampal HSP25 expression was studied by immunoblotting and immunohistochemistry (IHC). Expression of glial fibrillary acidic protein, nitrotyrosine, iNOS, HSP72, HSP90, and Cu/Zn superoxide dismutase (SOD) was assessed by either IHC or immunoblotting, Cu/Zn-SOD activity by enzymatic assay, and malondialdehyde (MDA) content by colorimetric assay. Hippocampal HSP25 was significantly increased in diabetic as compared to non-diabetic animals and localized predominantly within the pyramidal neurons layer of the CA1 area. This was paralleled by overexpression of nitrotyrosine, iNOS, SOD expression/activity, and enhanced MDA content. In experimental diabetes, HSP25 is overexpressed in the CA1 pyramidal neurons in parallel with markers of oxidative stress.
authors.affiliation: Diabetic Nephropathy Laboratory, Department of Internal Medicine, University of Turin, Italy. raffaella.mastrocola@unito.it
authors.forename: R
authors.initials: R
authors.lastname: Mastrocola
authors.forename: F
authors.initials: F
authors.lastname: Barutta
authors.forename: S
authors.initials: S
authors.lastname: Pinach
authors.forename: G
authors.initials: G
authors.lastname: Bruno
authors.forename: P Cavallo
authors.initials: PC
authors.lastname: Perin
authors.forename: G
authors.initials: G
authors.lastname: Gruden
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D013311:Streptozocin / Q000633:toxicity; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D005904:Glial Fibrillary Acidic Protein; D055551:HSP27 Heat-Shock Proteins; C486114:Hspb1 protein, rat; D008315:Malondialdehyde; D013311:Streptozocin; D052247:Nitric Oxide Synthase Type II; D013482:Superoxide Dismutase