title: Changes in protein profiles of multiple myeloma cells in response to bortezomib.
abstract: The objective of this study was to determine the changes in protein profiles of U-266 multiple myeloma cells in response to bortezomib. Bortezomib inhibited cell proliferation and increased the loss of mitochondrial membrane potential and caspase-3 activity in a dose-dependent manner. DECODON Delta2D Version 4.3 software demonstrated 37 differentially expressed protein spots: five proteins were newly formed, 10 proteins were lost, 12 proteins were up-regulated and 10 proteins were down-regulated in bortezomib-treated cells as compared to untreated cells. Some of the identified proteins after mass spectrometric analysis were as follows: apoptosis regulatory protein Siva (newly formed), caspase recruitment domain-containing protein 14 (lost), Ras-related protein Rab-25 (up-regulated), nuclear factor κB (NF-κB) p105 subunit (down-regulated). In summary, differentially expressed proteins of MM U-266 cells in response to bortezomib were analyzed and identified. The data obtained from this study may indicate the use of bortezomib for the treatment of various diseases.
authors.affiliation: Department of Chemistry, Faculty of Science, İzmir Institute of Technology, Urla, İzmir, Turkey.
authors.forename: Taylan
authors.initials: T
authors.lastname: Turan
authors.forename: Gülşah
authors.initials: G
authors.lastname: Sanlı-Mohamed
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Baran
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001897:Boronic Acids / Q000494:pharmacology*; D000069286:Bortezomib; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009101:Multiple Myeloma / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics; D011719:Pyrazines / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D001897:Boronic Acids; D020543:Proteome; D011719:Pyrazines; D000069286:Bortezomib; D053148:Caspase 3; D020782:Matrix Metalloproteinases