title: EGF-induced expansion of migratory cells in the rostral migratory stream.
abstract: The presence of neural stem cells in the adult brain is currently widely accepted and efforts are made to harness the regenerative potential of these cells. The dentate gyrus of the hippocampal formation, and the subventricular zone (SVZ) of the anterior lateral ventricles, are considered the main loci of adult neurogenesis. The rostral migratory stream (RMS) is the structure funneling SVZ progenitor cells through the forebrain to their final destination in the olfactory bulb. Moreover, extensive proliferation occurs in the RMS. Some evidence suggest the presence of stem cells in the RMS, but these cells are few and possibly of limited differentiation potential. We have recently demonstrated the specific expression of the cytoskeleton linker protein radixin in neuroblasts in the RMS and in oligodendrocyte progenitors throughout the brain. These cell populations are greatly altered after intracerebroventricular infusion of epidermal growth factor (EGF). In the current study we investigate the effect of EGF infusion on the rat RMS. We describe a specific increase of radixin(+)/Olig2(+) cells in the RMS. Negative for NG2 and CNPase, these radixin(+)/Olig2(+) cells are distinct from typical oligodendrocyte progenitors. The expanded Olig2(+) population responds rapidly to EGF and proliferates after only 24 hours along the entire RMS, suggesting local activation by EGF throughout the RMS rather than migration from the SVZ. In addition, the radixin(+)/Olig2(+) progenitors assemble in chains in vivo and migrate in chains in explant cultures, suggesting that they possess migratory properties within the RMS. In summary, these results provide insight into the adaptive capacity of the RMS and point to an additional stem cell source for future brain repair strategies.
authors.affiliation: Center for Brain Repair and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Olle R
authors.initials: OR
authors.lastname: Lindberg
authors.forename: Asa
authors.initials: A
authors.lastname: Persson
authors.forename: Anke
authors.initials: A
authors.lastname: Brederlau
authors.forename: Aidin
authors.initials: A
authors.lastname: Shabro
authors.forename: Hans Georg
authors.initials: HG
authors.lastname: Kuhn
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000008:administration & dosage*; D015870:Gene Expression / Q000187:drug effects; D007276:Injections, Intraventricular; D020646:Intralaminar Thalamic Nuclei / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology*; D009830:Olfactory Bulb / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D000076104:Oligodendrocyte Transcription Factor 2; D051381:Rats; D017208:Rats, Wistar
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D003598:Cytoskeletal Proteins; D004815:Epidermal Growth Factor; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; C522729:Olig2 protein, rat; C071776:radixin