title: Biochemical and bioaccumulation approaches for investigating marine pollution using Mediterranean rainbow wrasse, Coris julis (Linneaus 1798).
abstract: A multibiomarkers approach was used in order to estimate and monitor marine pollution. Coris julis (Linneaus, 1758) was chosen as a sentinel organism, and the specimens were collected from three well-known sites along the Ionic coast of Sicily: the protected marine area (P.M.A) "Cyclop's Islands" of Acitrezza (CT), used as a control site, Riposto (CT), and the industrial site of Augusta (SR). Abiotic levels of contaminants were also detected. High levels of biotic and abiotic accumulation were found at the industrial site in which the presence of genotoxic and oxidative damage were also evidenced, measured by Micronuclei, Alkaline and Fpg-modified Comet assays. The protein expression analysis showed metallothioneins (MTs) as good tissue-specific markers of metal accumulation. Their levels were significantly higher in muscle than in liver tissue for all the sampling sites, with a positive correlation among tissue levels and the degree of pollution at the sites. Conversely, heat shock proteins 70 (HSP70) expression was higher in Augusta and Riposto than in the control site, but no significant difference was found between the examined tissues among all sites.
authors.affiliation: Department of Drug's Sciences, Section of Biochemistry, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Tomasello
authors.forename: Chiara
authors.initials: C
authors.lastname: Copat
authors.forename: Valentina
authors.initials: V
authors.lastname: Pulvirenti
authors.forename: Venera
authors.initials: V
authors.lastname: Ferrito
authors.forename: Margherita
authors.initials: M
authors.lastname: Ferrante
authors.forename: Marcella
authors.initials: M
authors.lastname: Renis
authors.forename: Salvatore
authors.initials: S
authors.lastname: Sciacca
authors.forename: Concetta
authors.initials: C
authors.lastname: Tigano
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D001773:Blood Cells / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004784:Environmental Monitoring / Q000379:methods*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects; D006843:Hydrocarbons, Chlorinated / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D008668:Metallothionein / Q000378:metabolism; D008670:Metals / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D010473:Perciformes / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D012802:Sicily; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism* / Q000633:toxicity
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D006843:Hydrocarbons, Chlorinated; D008670:Metals; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D008668:Metallothionein; D011078:Polychlorinated Biphenyls