title: [1-butanol synthesis by Escherichia coli cells through butyryl-CoA formation by heterologous enzymes of clostridia and native enzymes of fatty acid beta-oxidation].
abstract: Anaerobic biosynthesis of 1-butanol from glucose is investigated in recombinant Escherichia coli strains which form butyryl-CoA using the heterologous enzyme complex of clostridia or as a result of a reversal in the action of native enzymes of the fatty acid beta-oxidation pathway. It was revealed that when the basic pathways of acetic and lactic acid formation are inactivated due to deletions in the ackA, pta, poxB, and ldhA genes, the efficiency of butyryl-CoA biosynthesis and its reduced product, i.e., 1-butanol, by two types of recombinant stains is comparable. The limiting factor for 1-butanol production by the obtained strains is the low substrate specificity of the basic CoA-dependent alcohol/aldehyde AdhE dehydrogenase from E. coli to butyryl-CoA. It was concluded that, in order to construct an efficient 1-butanol producer based on a model strain synthesizing butyryl-CoA as a result of a reversal in fatty acid beta-oxidation enzymes, it is necessary to provide intensive formation of acetyl-CoA and enhanced activity of alternative alcohol and aldehyde dehydrogenases in the cells of a strain.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Gulevich
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Skorokhodova
authors.forename: A A
authors.initials: AA
authors.lastname: Morzhakova
authors.forename: S V
authors.initials: SV
authors.lastname: Antonova
authors.forename: A V
authors.initials: AV
authors.lastname: Sukhozhenko
authors.forename: R S
authors.initials: RS
authors.lastname: Shakulov
authors.forename: V G
authors.initials: VG
authors.lastname: Debabov
mesh_terms: D020001:1-Butanol / Q000378:metabolism*; D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D017353:Gene Deletion; D005818:Genetic Engineering; D010084:Oxidation-Reduction; D016133:Polymerase Chain Reaction; D013379:Substrate Specificity
chemical_list: D000214:Acyl Coenzyme A; D001426:Bacterial Proteins; D017931:DNA Primers; D005227:Fatty Acids; C024343:butyryl-coenzyme A; D020001:1-Butanol; D015094:3-Hydroxyacyl CoA Dehydrogenases