title: Twins, tissue, and time: an assessment of SNPs and CNVs.
abstract: With the desire to assess genetic variation across the lifespan in large-scale collaborative projects, one question is whether inference of copy number (CN) is sensitive to the source of material for deoxyribonucleic acid (DNA) analysis (e.g., blood and buccal) and another question is whether CN is stable as individual sage. Here, we address these questions by applying Affymetrix 6.0 single nucleotide polymorphism (SNP)micro-arrays to 1,472 DNA samples from 710 individuals from the Netherlands Twin Register, including twin and non-twin individuals (372 with buccal and blood derived DNA and 388 with longitudinal data).Similar concordance for CN and genotype inference between samples from the same individual [or from the monozygotic (MZ) co-twins] was found for blood and buccal tissues. There was a small but statistically significant decrease in across-tissue concordance compared with concordance of samples from the same tissue type. No temporal effect was seen on CN variation from the 388 individuals sampled at two time points ranging from 1 to 12 years apart. The majority of our individuals were sampled at age younger than 20 years. Genotype concordance was very high (~ > 99%) between co-twins from 43 MZ pairs. For75 dizygotic (DZ) pairs, ~was ~65%. CN estimates were highly consistent between co-twins from MZ pairs for both deletions (f?2 ~ 90%) and duplications (~ ~ 86%). For DZ, these were similar for within-individual comparisons, but naturally lower between co-twins (~ ~ 50-60%). These results suggest that DNA from buccal samples perform as well as DNA from blood samples on the current generation of micro-array technologies.
authors.affiliation: Department of Epidemiology, University of Texas MD Anderson Cancer Center, Houston, TX, USA. pscheet@alum.wustl.edu
authors.forename: Paul
authors.initials: P
authors.lastname: Scheet
authors.forename: Erik A
authors.initials: EA
authors.lastname: Ehli
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Xiao
authors.forename: Catharina E M
authors.initials: CE
authors.lastname: van Beijsterveldt
authors.forename: Abdel
authors.initials: A
authors.lastname: Abdellaoui
authors.forename: Robert R
authors.initials: RR
authors.lastname: Althoff
authors.forename: Jouke Jan
authors.initials: JJ
authors.lastname: Hottenga
authors.forename: Gonneke
authors.initials: G
authors.lastname: Willemsen
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Nelson
authors.forename: Patricia E
authors.initials: PE
authors.lastname: Huizenga
authors.forename: Yueshan
authors.initials: Y
authors.lastname: Hu
authors.forename: Christopher I
authors.initials: CI
authors.lastname: Amos
authors.forename: Meike
authors.initials: M
authors.lastname: Bartels
authors.forename: Maria M
authors.initials: MM
authors.lastname: Groen-Blokhuis
authors.forename: Eco J C
authors.initials: EJ
authors.lastname: de Geus
authors.forename: James J
authors.initials: JJ
authors.lastname: Hudziak
authors.forename: Gareth E
authors.initials: GE
authors.lastname: Davies
authors.forename: Dorret I
authors.initials: DI
authors.lastname: Boomsma
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D056915:DNA Copy Number Variations; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D013048:Specimen Handling / Q000379:methods; D014430:Twins, Monozygotic / Q000235:genetics*
chemical_list: D004247:DNA