title: Enhancement of neurite outgrowth in neuron cancer stem cells by growth on 3-D collagen scaffolds.
abstract: Collagen is one component of the extracellular matrix that has been widely used for constructive remodeling to facilitate cell growth and differentiation. The 3-D distribution and growth of cells within the porous scaffold suggest a clinical significance for nerve tissue engineering. In the current study, we investigated proliferation and differentiation of neuron cancer stem cells (NCSCs) on a 3-D porous collagen scaffold that mimics the natural extracellular matrix. We first generated green fluorescence protein (GFP) expressing NCSCs using a lentiviral system to instantly monitor the transitions of morphological changes during growth on the 3-D scaffold. We found that proliferation of GFP-NCSCs increased, and a single cell mass rapidly grew with unrestricted expansion between days 3 and 9 in culture. Moreover, immunostaining with neuronal nuclei (NeuN) revealed that NCSCs grown on the 3-D collagen scaffold significantly enhanced neurite outgrowth. Our findings confirmed that the 80 μm porous collagen scaffold could enhance attachment, viability and differentiation of the cancer neural stem cells. This result could provide a new application for nerve tissue engineering and nerve regeneration.
authors.affiliation: Department of Electrical Engineering, I-Shou University, Taiwan, ROC.
authors.forename: Chih-Hao
authors.initials: CH
authors.lastname: Chen
authors.forename: Shyh Ming
authors.initials: SM
authors.lastname: Kuo
authors.forename: Guei-Sheung
authors.initials: GS
authors.lastname: Liu
authors.forename: Wan-Nan U
authors.initials: WN
authors.lastname: Chen
authors.forename: Chin-Wen
authors.initials: CW
authors.lastname: Chuang
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Liu
mesh_terms: D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000378:metabolism; D006801:Humans; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000378:metabolism; D016501:Neurites / Q000378:metabolism / Q000502:physiology*; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D054457:Tissue Scaffolds
chemical_list: D049452:Green Fluorescent Proteins; D003094:Collagen