title: Correlation between polymorphisms of nicotine acetylcholine acceptor             subunit CHRNA3 and lung cancer susceptibility.
abstract: Both environmental and genetic factors participate in the pathogenesis of             lung cancer. The aim of this study was to explore the association between CHRNA3             polymorphisms of the nicotinic acetylcholine receptor gene and lung cancer risk             in a hospital-based, case-controlled study. Single nucleotide polymorphisms (SNPs)             in CHRNA3 rs3743073 (A>G) were determined using the TaqMan-MGB probe technique             in 600 lung cancer cases and 600 normal controls. The differences in genotype             and allele frequency were compared between groups and their association with lung             cancer. The genotype frequency of rs3743073 (A>G) demonstrated Hardy-Weinberg             equilibrium (P<0.05). The genotype and allele frequencies were significantly             different between the cancer and control groups (P<0.05). Compared with patients             with the TT genotype, lung cancer incidence was increased in patients with the             TG and GG genotypes (OR=1.68; 95% CI, 1.30-2.19; P<0.05; OR=1.30; 95% CI, 1.05-1.61;             P<0.05, respectively). Patients with rs3743073G variant alleles (TG and GG)             were at greater risk (OR=0.65; 95% CI, 0.50-0.84; P<0.05) of developing lung             cancer. Increased risk associated with rs3743073G variant alleles was observed             in male smokers over the age of 60 (P<0.05). In this cohort, the CHRNA3 gene             rs3743073G variant genotype significantly increased lung cancer risk, especially             in male smokers over the age of 60.
authors.affiliation: Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu                     210009, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Shen
authors.forename: Mei-Qi
authors.initials: MQ
authors.lastname: Shi
authors.forename: Ma-Qing
authors.initials: MQ
authors.lastname: Zheng
authors.forename: Sai-Nan
authors.initials: SN
authors.lastname: Hu
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Feng
mesh_terms: D000367:Age Factors; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D015331:Cohort Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015994:Incidence; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011978:Receptors, Nicotinic / Q000235:genetics*; D012307:Risk Factors; D012737:Sex Factors; D012907:Smoking
chemical_list: D011978:Receptors, Nicotinic