title: [Effect of artemisinin on the expressions of GRalpha mRNA, GRbeta mRNA and P300/CBP protein in lupus nephritis mice].
abstract: OBJECTIVE
  To investigate the effects of Artemisinin on the expressions of GRalpha mRNA, GRbeta mRNA and P300/CBP protein in lupus nephritis mice.
  
  
  METHODS
  Forty hybrid female mice were randomly and equally divided into four groups with the method of random number table: control group, model group, prednisone group administrated with 6.45 mg/kg Artemisinin (Art) suspension. A mice model of LN was established by injection with living lymph cell suspension. The expressions of GC receptor alpha (GRalpha) mRNA, GC receptor beta (GRbeta) mRNA in peripheral blood mononuclear cells (PBMCs), and transcriptional coactivator P300/CBP protein in renal tissue were respectively measured by the technique of RT - PCR and immunohistochemical assay.
  
  
  RESULTS
  Compared with the model group. The expression of transcriptional coactivator P300/CBP protein in renal tissue and GRa mRNA in PBMCs of treatment groups was increased significantly, GRbeta mRNA expression was significantly decreased (P < 0.05 or P < 0.01). And the Art group had a better effect on the expression of GRalpha mRNA and transcriptional coactivator P300/CBP protein than that of the prednisone group (P < 0.01).
  
  
  CONCLUSIONS
  The underlying therapeutic mechanism may be correlated with the regulation of Art on the expressions of GRalpha mRNA, GRbeta mRNA in PBMC and transcriptional coactivator P300/CBP protein in renal tissue.
authors.affiliation: Department of Integrated Chinese Traditional and Western Medicine, Shaanxi Province Key TCM Department of Nephrology, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710004, China. wuxili1984@163.com
authors.forename: Xi-Li
authors.initials: XL
authors.lastname: Wu
authors.forename: Wan-Sen
authors.initials: WS
authors.lastname: Sun
authors.forename: Xing-Min
authors.initials: XM
authors.lastname: Shi
authors.forename: Zhu
authors.initials: Z
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: An
authors.forename: Cheng-Lin
authors.initials: CL
authors.lastname: Qiao
mesh_terms: D000818:Animals; D031172:Artemisia annua / Q000737:chemistry; D037621:Artemisinins / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008181:Lupus Nephritis / Q000188:drug therapy / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D011241:Prednisone / Q000008:administration & dosage / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050880:p300-CBP Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D037621:Artemisinins; D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; C411470:glucocorticoid receptor alpha; C121943:glucocorticoid receptor beta; C031327:artemisinin; D050880:p300-CBP Transcription Factors; D011241:Prednisone