title: Telomere shortening is associated with reduced duodenal HCOFormula secretory but normal gastric acid secretory capacity in aging mice.
abstract: The incidence of duodenal ulcer, especially Helicobacter pylori-negative duodenal ulcer, strongly increases with age. In humans, telomere length shortening is considered to be one critical factor in cellular senescence and organ survival. In this study, we compared basal and stimulated gastric acid and duodenal HCO(3)(-) secretory rates in aged late-generation (G(3)) telomerase-deficient (mTERC(-/-)) mice, which are characterized by severe telomere dysfunction due to the inability to elongate telomeres during cell division. We found that basal and forskolin-stimulated HCO(3)(-) secretion and short-circuit current (I(sc)) in isolated duodenal mucosa of G(3) mTERC(-/-) mice were markedly reduced compared with age-matched wild-type mice. In contrast, basal and forskolin-stimulated acid secretory rates in isolated G(3) mTERC(-/-) gastric mucosa were not significantly altered. Correspondingly, duodenal mucosa of G(3) mTERC(-/-) mice showed slimming and shortening of villi, whereas gastric mucosal histology was not significantly altered. However, the ratios of cystic fibrosis transmembrane conductance regulator (CFTR) and solute-linked carrier 26 gene family (Slc26a6) mRNA expression in relation to cytokeratin-18 were not altered in duodenal mucosa. The further knockout of p21, which is a downstream effector of telomere shortening-induced senescence, rescued villus atrophy of duodenal mucosa, and basal and forskolin-stimulated duodenal HCO(3)(-) secretion and I(sc) in mTERC(-/-) p21(-/-) double-knockout mice were not different from wild-type controls. In conclusion, genetic ablation of telomerase resulted in p21-dependent duodenal mucosal atrophy and reduced duodenal HCO(3)(-) secretory capacity, whereas gastric morphology and acid secretory function were preserved. This suggests that telomere shortening during aging may result in an imbalance between aggressive and protective secretions against duodenal mucosa and thus predispose to ulcer formation.
authors.affiliation: Dept. of Gastroenterology, Affiliated Hospital, Zunyi Medical College, Dalian Rd. 149, Zunyi 563003, China. tuobiguang@yahoo.com.cn
authors.forename: Biguang
authors.initials: B
authors.lastname: Tuo
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Ju
authors.forename: Brigitte
authors.initials: B
authors.lastname: Riederer
authors.forename: Regina
authors.initials: R
authors.lastname: Engelhardt
authors.forename: Michael P
authors.initials: MP
authors.lastname: Manns
authors.forename: K Lenhard
authors.initials: KL
authors.lastname: Rudolph
authors.forename: Ursula
authors.initials: U
authors.lastname: Seidler
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism*; D004386:Duodenum / Q000503:physiopathology*; D005744:Gastric Acid / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D059506:Telomere Shortening / Q000502:physiology*
chemical_list: D001639:Bicarbonates