title: Interactions of sulforaphane and dimethyl sulfoxide with methyl methanesulfonate, urethane, 4-nitroquinoline-1-oxide and hydrogen peroxide in the Drosophila melanogaster wing spot test.
abstract: Sulforaphane (SF) is an isothiocyanate present in Brassicaceae, vegetables that induce the detoxification of electrophiles and reactive oxygen species. SF has been correlated with chemoprevention mechanisms against degenerative diseases. We tested if the SF had an effect against methyl methanesulfonate (MMS), urethane (URE), 4-NQO and H(2)O(2). SF (>95% purity, 0.14, 0.28, 0.56 mM) was diluted in a DMSO/Tw80/EtOH mixture (DTE) corresponding to 25, 50, 100% of lyophilized broccoli. The SF treatment (0.14 mM) was positive for small spots in the ST cross and negative in the HB cross. In the HB cross, SF (0.28 mM) was genotoxic. In the ST cross, the SF treatments showed a tendency to reduce the genotoxic damage caused by MMS, which could be explained by the radical scavenging action of the DTE mixture. In the ST cross, the frequency of small spots in the SF 0.14 mM/URE treatment was similar to that of Water/URE, which can be explained by a DTE and SF scavenger action. In both crosses, the results for the direct oxidants, 4-NQO and H(2)O(2), were different and must be related to differential modulation of CYPs expression and the SF and DTE scavenger properties.
authors.affiliation: Genetic Toxicology, FES Iztacala, Universidad Nacional Autónoma de México, Los Reyes Iztacala, Tlalnepantla, Estado de México, Mexico.
authors.forename: I E
authors.initials: IE
authors.lastname: Dueñas-García
authors.forename: L F
authors.initials: LF
authors.lastname: Santos-Cruz
authors.forename: L
authors.initials: L
authors.lastname: Castañeda-Partida
authors.forename: A N
authors.initials: AN
authors.lastname: Castañeda-Sortibrán
authors.forename: M G
authors.initials: MG
authors.lastname: Ordaz-Téllez
authors.forename: A
authors.initials: A
authors.lastname: Sánchez-Santos
authors.forename: A
authors.initials: A
authors.lastname: Durán-Díaz
authors.forename: R
authors.initials: R
authors.lastname: Rodríguez-Arnaiz
authors.forename: M E
authors.initials: ME
authors.lastname: Heres-Pulido
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology*; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology; D019607:Brassicaceae / Q000737:chemistry; D004249:DNA Damage; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004331:Drosophila melanogaster / Q000187:drug effects*; D004347:Drug Interactions; D005260:Female; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D017879:Isothiocyanates / Q000494:pharmacology; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology; D013454:Sulfoxides; D013861:Thiocyanates / Q000494:pharmacology*; D014520:Urethane / Q000494:pharmacology*
chemical_list: D016588:Anticarcinogenic Agents; D017879:Isothiocyanates; D010936:Plant Extracts; D013454:Sulfoxides; D013861:Thiocyanates; C037152:isothiocyanic acid; D014520:Urethane; D015112:4-Nitroquinoline-1-oxide; D006861:Hydrogen Peroxide; C016766:sulforaphane; D004121:Dimethyl Sulfoxide