title: Confinement to organelle-associated inclusion structures mediates asymmetric inheritance of aggregated protein in budding yeast.
abstract: The division of the S. cerevisiae budding yeast, which produces one mother cell and one daughter cell, is asymmetric with respect to aging. Remarkably, the asymmetry of yeast aging coincides with asymmetric inheritance of damaged and aggregated proteins by the mother cell. Here, we show that misfolded proteins are retained in the mother cell by being sequestered in juxtanuclear quality control compartment (JUNQ) and insoluble protein deposit (IPOD) inclusions, which are attached to organelles. Upon exposure to stress, misfolded proteins accumulate in stress foci that must be disaggregated by Hsp104 in order to be degraded or processed to JUNQ and IPOD. Cells that fail to deliver aggregates to an inclusion pass on aggregates to subsequent generations.
authors.affiliation: Department of Cell and Developmental Biology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors.forename: Rachel
authors.initials: R
authors.lastname: Spokoini
authors.forename: Ofer
authors.initials: O
authors.lastname: Moldavski
authors.forename: Yaakov
authors.initials: Y
authors.lastname: Nahmias
authors.forename: Jeremy L
authors.initials: JL
authors.lastname: England
authors.forename: Maya
authors.initials: M
authors.lastname: Schuldiner
authors.forename: Daniel
authors.initials: D
authors.lastname: Kaganovich
mesh_terms: D002455:Cell Division; D006360:Heat-Shock Proteins / Q000378:metabolism; D015388:Organelles / Q000378:metabolism*; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D006360:Heat-Shock Proteins; D029701:Saccharomyces cerevisiae Proteins; C070972:HsP104 protein, S cerevisiae