title: A sulfated polysaccharide, fucans, isolated from brown algae Sargassum vulgare with anticoagulant, antithrombotic, antioxidant and anti-inflammatory effects.
abstract: Fucan (SV1) sulfated polysaccharides from the brown algae Sargassum vulgare were extracted, fractionated in acetone and examined with respect to chemical composition, anticoagulant, anti-inflammatory, antithrombotic effects and cellular proliferation. These polysaccharides contain low levels of protein, high level of carbohydrate and sulfate. Monosaccharides analysis revealed that SV1 was composed of fucose, galactose, xylose, glucuronic acid and mannose. SV1 polysaccharide prolonged activated partial thromboplastin time (aPTT) and exhibited high antithrombotic action in vivo, with a concentration ten times higher than heparin activity. PSV1, a purified form in gel filtration showed very low biological activities. SV1 stimulated the enzymatic activity of FXa. Its action on DPPH radical scavenging activity was 22%. This polymer has no cytotoxic action (hemolytic) on ABO and Rh blood types in different erythrocyte groups. It displays strong anti-inflammatory action at all concentrations tested in the carrageenan-induced paw edema model, demonstrated by reduced edema and cellular infiltration.
authors.affiliation: Laboratory of Glycobiology, Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Av. Sen Salgado Filho, No. 3000, Natal, RN, Brazil.
authors.forename: Celina Maria P Guerra
authors.initials: CM
authors.lastname: Dore
authors.forename: Monique Gabriela
authors.initials: MG
authors.lastname: das C Faustino Alves
authors.forename: Luiza Sheyla E Pofírio
authors.initials: LS
authors.lastname: Will
authors.forename: Thiago G
authors.initials: TG
authors.lastname: Costa
authors.forename: Diego A
authors.initials: DA
authors.lastname: Sabry
authors.forename: Leonardo Augusto R
authors.initials: LA
authors.lastname: de Souza Rêgo
authors.forename: Camila M
authors.initials: CM
authors.lastname: Accardo
authors.forename: Hugo Alexandre O
authors.initials: HA
authors.lastname: Rocha
authors.forename: Luciana Guimarães A
authors.initials: LG
authors.lastname: Filgueira
authors.forename: Edda Lisboa
authors.initials: EL
authors.lastname: Leite
mesh_terms: D000017:ABO Blood-Group System / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D002351:Carrageenan / Q000009:adverse effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology; D015951:Factor Xa / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D006461:Hemolysis / Q000187:drug effects; D008297:Male; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012204:Rh-Hr Blood-Group System / Q000378:metabolism; D044647:Sargassum / Q000737:chemistry*; D013917:Thrombin / Q000378:metabolism
chemical_list: D000017:ABO Blood-Group System; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000925:Anticoagulants; D005343:Fibrinolytic Agents; D016166:Free Radical Scavengers; D011134:Polysaccharides; D012204:Rh-Hr Blood-Group System; D002351:Carrageenan; C007789:fucoidan; D013917:Thrombin; D015951:Factor Xa