title: HPLC methods for analysis of porphyrins in biological media.
abstract: Changes in porphyrin concentrations in biological media may serve as biological indicators of exposure and toxicity from a wide variety of drugs and chemical agents. This unit describes procedures for quantitative extraction of porphyrins from urine, feces, blood, and biological tissues as well as their separation and analysis by HAPLY spectrofluorometrc techniques.
authors.affiliation: University of Washington, Seattle, Washington, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Woods
authors.forename: P L
authors.initials: PL
authors.lastname: Simmonds
mesh_terms: D000818:Animals; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry; D006801:Humans; D011166:Porphyrins / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D013050:Spectrometry, Fluorescence
chemical_list: D011166:Porphyrins