title: Adenovirus-mediated bone morphogenetic protein-2 gene transfection of bone marrow mesenchymal stem cells combined with nano-hydroxyapatite to construct bone graft material in vitro.
abstract: To study the adhesion, proliferation and expression of bone marrow mesenchymal stem cells (BMSCs) on nano-hydroxyapatite (Nano-HA) bone graft material after transfection of adenovirus-mediated human bone morphogenetic protein-2 expression vector (Ad-BMP-2). BMSCs were transfected using Ad-BMP-2. Immunohistochemistry and Western blot were used to detect BMP-2 expression in transfected cells. After transfection, BMP-2 protein was highly expressed in BMSCs; MTT test assay showed that the Nano-HA bone graft material could not inhibit in vitro proliferation of BMSCs. Ad-BMP-2-transfected BMSCs are well biocompatible with Nano-HA bone graft material, the transfected cells in material can secrete BMP-2 stably for a long time.
authors.affiliation: Department of Trauma Orthopedics, Second People's Hospital of Shenzhen, Guangdong Province, P. R. China.
authors.forename: W C
authors.initials: WC
authors.lastname: Li
authors.forename: D P
authors.initials: DP
authors.lastname: Wang
authors.forename: L J
authors.initials: LJ
authors.lastname: Li
authors.forename: W M
authors.initials: WM
authors.lastname: Zhu
authors.forename: Y J
authors.initials: YJ
authors.lastname: Zeng
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D055396:Bone Morphogenetic Protein 2 / Q000235:genetics* / Q000378:metabolism; D018786:Bone Substitutes / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression; D005822:Genetic Vectors; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D049329:Nanostructures; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014162:Transfection
chemical_list: D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D017886:Durapatite