title: Soluble CD14 subtype (sCD14-ST) presepsin in critically ill preterm newborns: preliminary reference ranges.
abstract: Soluble CD14 subtype (sCD14-ST), also named presepsin, is a 13 kDa truncated form of soluble CD14 (sCD14), consisting of 64 amino acid residues. Systemic inflammation and sepsis are characterized by an early, significant increase in sCD14-ST presepsin blood concentration and thus, this small polypeptide has been proposed as a novel, reliable biomarker for the management of sepsis. We enrolled twenty-six consecutive non-septic preterm newborns with gestational age (GA) between 26 and 36 weeks) admitted to NICU after the first day of life for various severe diseases. sCD14-ST presepsin was measure on whole blood samples by a rapid commercial available chemiluminescent enzyme immunoassay (CLEIA) based on a non-competitive CLEIA. The mean sCD14-ST presepsin blood level in 26 preterm newborns was 643.1 ng/L, with a standard deviation (SD) of 303.8 ng/L; the median value was 578 ng/L. Our results clearly suggest no correlation between GA and sCD14-ST presepsin blood level between 26 and 36 weeks and thus it is reasonable to adopt a unique reference range for preterm newborns.
authors.affiliation: Department of Laboratory Medicine, IRCCS University-Hospital San Martino-IST, National Institute for Cancer Research, Largo Rosanna Benzi 11, Genoa, Italy. michele.mussap@hsanmartino.it
authors.forename: Michele
authors.initials: M
authors.lastname: Mussap
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Puxeddu
authors.forename: Patrizia
authors.initials: P
authors.lastname: Burrai
authors.forename: Antonio
authors.initials: A
authors.lastname: Noto
authors.forename: Francesco
authors.initials: F
authors.lastname: Cibecchini
authors.forename: Marcella
authors.initials: M
authors.lastname: Testa
authors.forename: Melania
authors.initials: M
authors.lastname: Puddu
authors.forename: Giovanni
authors.initials: G
authors.lastname: Ottonello
authors.forename: Angelica
authors.initials: A
authors.lastname: Dessì
authors.forename: Roberta
authors.initials: R
authors.lastname: Irmesi
authors.forename: Elisabetta Dalla
authors.initials: ED
authors.lastname: Gassa
authors.forename: Claudia
authors.initials: C
authors.lastname: Fanni
authors.forename: Vassilios
authors.initials: V
authors.lastname: Fanos
mesh_terms: D005260:Female; D005865:Gestational Age; D006801:Humans; D007124:Immunoenzyme Techniques; D007231:Infant, Newborn; D007234:Infant, Premature; D007235:Infant, Premature, Diseases / Q000097:blood*; D007249:Inflammation / Q000097:blood; D015931:Intensive Care, Neonatal; D018950:Lipopolysaccharide Receptors / Q000097:blood*; D008163:Luminescent Measurements; D008297:Male; D011379:Prognosis; D012016:Reference Values; D018805:Sepsis / Q000097:blood
chemical_list: D018950:Lipopolysaccharide Receptors