title: Recombinant envelope protein-based enzyme immunoassay for IgG antibodies is comparable to neutralization tests for epidemiological studies of dengue infection.
abstract: Dengue virus (DENV) is the most prevalent arbovirus in the world, found mainly in tropical regions. As clinical manifestations present frequently as nonspecific febrile illness, laboratory diagnosis is essential to confirm DENV infections and for epidemiological studies. Recombinant envelope (E) antigens of four serotypes of DENV were used to develop an immunoglobulin G enzyme-linked immunosorbent assay (IgG-ELISA). To evaluate the IgG-ELISA, a panel of serum samples that had been tested previously by a plaque reduction neutralization test (PRNT) was investigated for the presence of anti-E antibodies against the four DENV serotypes. IgG-ELISA was found to have a sensitivity (91%) and specificity (98%) at a receiver-operating characteristic (ROC) optimized cutoff and demonstrated high performance as well as good indexes. A concordance of 97% was achieved between both assays, and only 21/704 (3%) samples were not concordant. The results of the present study demonstrate a moderate correlation between neutralizing antibody titers and IgG-ELISA values. These findings indicate that the recombinant protein-based IgG-ELISA is a suitable method for routine serodiagnosis, monitoring and seroepidemiological studies of DENV infections.
authors.affiliation: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Laboratório de Vírus, Belo Horizonte, MG, Brazil.
authors.forename: Eliseu Soares de Oliveira
authors.initials: ES
authors.lastname: Rocha
authors.forename: Jaquelline Germano de
authors.initials: JG
authors.lastname: Oliveira
authors.forename: João Rodrigues dos
authors.initials: JR
authors.lastname: Santos
authors.forename: Gisele Olinto Libânio
authors.initials: GO
authors.lastname: Rodrigues
authors.forename: Leandra Barcelos
authors.initials: LB
authors.lastname: Figueiredo
authors.forename: José Eduardo Marques
authors.initials: JE
authors.lastname: Pessanha
authors.forename: Fernando Augusto
authors.initials: FA
authors.lastname: Proietti
authors.forename: Flávio Guimarães da
authors.initials: FG
authors.lastname: Fonseca
authors.forename: Claudio Antonio
authors.initials: CA
authors.lastname: Bonjardim
authors.forename: Paulo Cesar Peregrino
authors.initials: PC
authors.lastname: Ferreira
authors.forename: Erna Geessien
authors.initials: EG
authors.lastname: Kroon
mesh_terms: D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D012698:Serologic Tests; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins