title: Influence of dark phase restricted high fat feeding on myocardial adaptation in mice.
abstract: Prolonged high fat feeding is associated with myocardial contractile dysfunction in rodents. However, epidemiological data do not necessarily support the concept that fat-enriched diets adversely affect cardiac function in humans. When fed in an ad libitum manner, laboratory rodents consume chow throughout the day. In contrast, humans typically consume food only during the awake phase. Discrepancies between rodent and human feeding behaviors led us to hypothesize that the time of day at which dietary lipids are consumed significantly influences myocardial adaptation. In order to better mimic feeding behavior in humans, mice were fed (either a control or high fat diet) only during the 12-hour dark phase (i.e., no food was provided during the light phase). We report that compared to dark phase restricted control diet fed mice, mice fed a high fat diet during the dark phase exhibit: 1) essentially normal body weight gain and energy balance; 2) increased fatty acid oxidation at whole body, as well as skeletal and cardiac muscle (in the presence of insulin and/or at high workloads) levels; 3) induction of fatty acid responsive genes, including genes promoting triglyceride turnover in the heart; 4) no evidence of cardiac hypertrophy; and 5) persistence/improvement of myocardial contractile function, as assessed ex vivo. These data are consistent with the hypothesis that ingestion of dietary fat only during the more active/awake period allows adequate metabolic adaptation, thereby preserving myocardial contractile function. This article is part of a Special Issue entitled "Focus on cardiac metabolism".
authors.affiliation: USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, Department of Pediatrics, Houston, TX, USA.
authors.forename: Ju-Yun
authors.initials: JY
authors.lastname: Tsai
authors.forename: Carolina
authors.initials: C
authors.lastname: Villegas-Montoya
authors.forename: Brandon B
authors.initials: BB
authors.lastname: Boland
authors.forename: Zachary
authors.initials: Z
authors.lastname: Blasier
authors.forename: Oluwaseun
authors.initials: O
authors.lastname: Egbejimi
authors.forename: Raquel
authors.initials: R
authors.lastname: Gonzalez
authors.forename: Michael
authors.initials: M
authors.lastname: Kueht
authors.forename: Tracy A
authors.initials: TA
authors.lastname: McElfresh
authors.forename: Rachel A
authors.initials: RA
authors.lastname: Brewer
authors.forename: Margaret P
authors.initials: MP
authors.lastname: Chandler
authors.forename: Molly S
authors.initials: MS
authors.lastname: Bray
authors.forename: Martin E
authors.initials: ME
authors.lastname: Young
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D004435:Eating; D004734:Energy Metabolism; D005227:Fatty Acids / Q000378:metabolism; D006321:Heart / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D014158:Transcription, Genetic
chemical_list: D005227:Fatty Acids