title: Skin- and gut-homing molecules on human circulating γδ T cells and their dysregulation in inflammatory bowel disease.
abstract: Changes in phenotype and function of γδ T cells have been reported in inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC). Dysregulation of lymphocyte migration plays a key role in IBD pathogenesis; however, data on migratory properties of γδ T cells are scarce. Human circulating γδ T cells from healthy controls (n = 27), patients with active CD (n = 15), active UC (n = 14) or cutaneous manifestations of IBD (n = 2) were characterized by flow cytometry. Circulating γδ T cells in healthy controls were CD3(hi)  and expressed CD45RO. They expressed gut-homing molecule β7 but not gut-homing molecule corresponding chemokine receptors (CCR)9, or skin-homing molecules cutaneous lymphocyte-associated antigen (CLA) and CCR4, despite conventional T cells containing populations expressing these molecules. CCR9 expression was increased on γδ T cells in CD and UC, while skin-homing CLA was expressed aberrantly on γδ T cells in patients with cutaneous manifestations of IBD. Lower levels of CD3 expression were found on γδ T cells in CD but not in UC, and a lower proportion of γδ T cells expressed CD45RO in CD and UC. Enhanced expression of gut-homing molecules on circulating γδ T cells in IBD and skin-homing molecules in cutaneous manifestations of IBD may be of clinical relevance.
authors.affiliation: Antigen Presentation Research Group, Imperial College London, Northwick Park and St Mark’s Campus, Level 7W, St Mark’s Hospital,Watford Road, Harrow HA1 3UJ, UK.
authors.forename: E R
authors.initials: ER
authors.lastname: Mann
authors.forename: N E
authors.initials: NE
authors.lastname: McCarthy
authors.forename: S T C
authors.initials: ST
authors.lastname: Peake
authors.forename: A N
authors.initials: AN
authors.lastname: Milestone
authors.forename: H O
authors.initials: HO
authors.lastname: Al-Hassi
authors.forename: D
authors.initials: D
authors.lastname: Bernardo
authors.forename: C T
authors.initials: CT
authors.lastname: Tee
authors.forename: J
authors.initials: J
authors.lastname: Landy
authors.forename: M C
authors.initials: MC
authors.lastname: Pitcher
authors.forename: S A
authors.initials: SA
authors.lastname: Cochrane
authors.forename: A L
authors.initials: AL
authors.lastname: Hart
authors.forename: A J
authors.initials: AJ
authors.lastname: Stagg
authors.forename: S C
authors.initials: SC
authors.lastname: Knight
mesh_terms: D000328:Adult; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology / Q000378:metabolism; D017252:CD3 Complex / Q000276:immunology / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000276:immunology / Q000378:metabolism; D003424:Crohn Disease / Q000276:immunology / Q000378:metabolism*; D005260:Female; D041981:Gastrointestinal Tract / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000276:immunology* / Q000378:metabolism; D039641:Integrin beta Chains / Q000276:immunology / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000276:immunology / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism; D054388:Receptors, CCR / Q000276:immunology / Q000378:metabolism; D054398:Receptors, CCR4 / Q000276:immunology / Q000378:metabolism; D012867:Skin / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; C119309:CC chemokine receptor 9; C515354:CCR4 protein, human; D017252:CD3 Complex; C063419:CTAGE1 protein, human; D039641:Integrin beta Chains; D008562:Membrane Glycoproteins; D054388:Receptors, CCR; D054398:Receptors, CCR4; C091368:integrin beta7; D017493:Leukocyte Common Antigens