title: Quantitative phase imaging using a partitioned detection aperture.
abstract: We present a technique to quantitatively image the phase of thin quasi-transparent samples using extended source incoherent illumination and off-axis detection apertures. Our technique is achromatic and polarization independent, requires no active elements, and can be readily adapted to standard bright-field microscopes. We demonstrate our technique by quantitatively reconstructing the phase of cheek cells and a microlens. The light efficient, single-shot nature of our technique enables phase imaging at frame rates that are camera limited.
authors.affiliation: Boston University, Department of Biomedical Engineering, 44 Cummington Street, Boston, Massachusetts 02215, USA.
authors.forename: Ashwin B
authors.initials: AB
authors.lastname: Parthasarathy
authors.forename: Kengyeh K
authors.initials: KK
authors.lastname: Chu
authors.forename: Tim N
authors.initials: TN
authors.lastname: Ford
authors.forename: Jerome
authors.initials: J
authors.lastname: Mertz
mesh_terms: D002138:Calibration; D002610:Cheek; D004867:Equipment Design; D061848:Optical Imaging / Q000295:instrumentation*