title: Isolation of neonatal rat cortical astrocytes for primary cultures.
abstract: There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.
authors.affiliation: Wake Forest University School of Medicine, Winston-Salem, North Carolina, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Allen
authors.forename: L A
authors.initials: LA
authors.lastname: Mutkus
authors.forename: M
authors.initials: M
authors.lastname: Aschner
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000166:cytology*; D018929:Cell Culture Techniques; D002540:Cerebral Cortex / Q000166:cytology*; D003470:Culture Media; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003470:Culture Media