title: Experimental model of tooth movement in mice: a standardized protocol for studying bone remodeling under compression and tensile strains.
abstract: During orthodontic tooth movement (OTM), alveolar bone is resorbed by osteoclasts in compression sites (CS) and is deposited by osteoblasts in tension sites (TS). The aim of this study was to develop a standardized OTM protocol in mice and to investigate the expression of bone resorption and deposition markers in CS and TS. An orthodontic appliance was placed in C57BL6/J mice. To define the ideal orthodontic force, the molars of the mice were subjected to forces of 0.1N, 0.25 N, 0.35 N and 0.5 N. The expression of mediators that are involved in bone remodeling at CS and TS was analyzed using a Real-Time PCR. The data revealed that a force of 0.35 N promoted optimal OTM and osteoclast recruitment without root resorption. The levels of TNF-α, RANKL, MMP13 and OPG were all altered in CS and TS. Whereas TNF-α and Cathepsin K exhibited elevated levels in CS, RUNX2 and OCN levels were higher in TS. Our results suggest that 0.35 N is the ideal force for OTM in mice and has no side effects. Moreover, the expression of bone remodeling markers differed between the compression and the tension areas, potentially explaining the distinct cellular migration and differentiation patterns in each of these sites.
authors.affiliation: Department of Oral Pathology, Faculty of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Silvana Rodrigues de Albuquerque
authors.initials: SR
authors.lastname: Taddei
authors.forename: Adriana Pedrosa
authors.initials: AP
authors.lastname: Moura
authors.forename: Ildeu
authors.initials: I
authors.lastname: Andrade
authors.forename: Gustavo Pompermaier
authors.initials: GP
authors.lastname: Garlet
authors.forename: Thiago Pompermaier
authors.initials: TP
authors.lastname: Garlet
authors.forename: Mauro Martins
authors.initials: MM
authors.lastname: Teixeira
authors.forename: Tarcília Aparecida
authors.initials: TA
authors.lastname: da Silva
mesh_terms: D000818:Animals; D016723:Bone Remodeling / Q000502:physiology*; D056657:Cathepsin K / Q000235:genetics; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D015675:Osteocalcin / Q000235:genetics; D053244:Osteoprotegerin / Q000235:genetics; D053245:RANK Ligand / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000235:genetics; D013314:Stress, Mechanical; D014087:Tooth Movement Techniques; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D053246:Receptor Activator of Nuclear Factor-kappa B; C493716:Runx2 protein, mouse; C505687:Tnfrsf11a protein, mouse; C506031:Tnfrsf11b protein, mouse; D014409:Tumor Necrosis Factor-alpha; D015675:Osteocalcin; D016753:Interleukin-10; D056657:Cathepsin K; D053509:Matrix Metalloproteinase 13; C507081:Mmp13 protein, mouse