title: Fabrication of GO/PANi/CdSe nanocomposites for sensitive electrochemiluminescence biosensor.
abstract: A novel graphene oxide sheets/polyaniline/CdSe quantum dots (GO/PANi/CdSe) nanocomposites were successfully synthesized and used for the sensitive electrochemiluminescence (ECL) biosensing. The GO/PANi/CdSe nanocomposites were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), ultraviolet-visible (UV-vis) absorption spectroscopy, photoluminescence (PL) spectroscopy and Fourier transform infrared (FTIR) spectroscopy. Finally, the nanocomposites were employed to construct the biosensor via layer-by-layer assembly for the ECL detection of Cytochrome C (Cyt C). The whole process was characterized by cyclic voltammogram (CV) and electrochemical impedance spectroscopy (EIS). Experimental parameters such as the ratio of GO/PANi, the K(2)S(4)O(8) concentration and the pH value of electrolyte solution were studied to investigate the effect on the ECL intensity. Under the optimized conditions, the ECL intensity decreased linearly with the Cyt C concentrations in the range from 5.0×10(-8) to 1.0×10(-4) M with detection limit of 2.0×10(-8) M. Besides, the as-proposed biosensor exhibits high specificity, good reproducibility, and stability, and may be applied in more bioanalytical systems.
authors.affiliation: School of Chemistry and Chemical Engineering, Anhui University, Hefei 230039, PR China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Hu
authors.forename: Chang-Jie
authors.initials: CJ
authors.lastname: Mao
authors.forename: Ji-Ming
authors.initials: JM
authors.lastname: Song
authors.forename: He-Lin
authors.initials: HL
authors.lastname: Niu
authors.forename: Sheng-Yi
authors.initials: SY
authors.lastname: Zhang
authors.forename: Hai-ping
authors.initials: HP
authors.lastname: Huang
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D019187:Cadmium Compounds / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D045304:Cytochromes c / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006108:Graphite / Q000737:chemistry*; D008163:Luminescent Measurements / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results; D018036:Selenium Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D000814:Aniline Compounds; D019187:Cadmium Compounds; D010087:Oxides; D018036:Selenium Compounds; C416807:polyaniline; D006108:Graphite; D045304:Cytochromes c; C058667:cadmium selenide