title: An RNA-deaminase conjugate selectively repairs point mutations.
abstract: Checking for mistakes: By conjugating a catalytic domain with a guide RNA, deamination activity can be harnessed to repair a specific codon on mRNA. This method can be used for the highly selective repair of point mutations in mRNA by site-selective editing.
authors.affiliation: Interfaculty Institute of Biochemistry, University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany. thorsten.stafforst@uni-tuebingen.de
authors.forename: Thorsten
authors.initials: T
authors.lastname: Stafforst
authors.forename: Marius F
authors.initials: MF
authors.lastname: Schneider
mesh_terms: D000243:Adenosine Deaminase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008958:Models, Molecular; D015394:Molecular Structure; D009700:Nucleoside Deaminases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017354:Point Mutation; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D012313:RNA; D009700:Nucleoside Deaminases; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase