title: Preparation and evaluation of nanoparticles for directed tissue engineering.
abstract: Herein we describe the preparation of a nanoparticulate system formed from an RGD-functionalized chitosan derivative by complexation with chondroitin sulfate. These bioactive complexes were developed to promote wound healing by inducing adhesion and subsequently migration of skin cells. The particles were characterized for their size, surface charge, stability and shape. Briefly, the nanoparticles were found to be stable up to 7 days in water at a diameter of 150-200 nm and a positive charge of 20 mV. In physiological media the particles swell significantly but remain intact. Tested in an in vitro cell model of human dermal fibroblasts, the particles were shown to promote cell adhesion and induce spreading in human dermal fibroblasts. The mean surface area per cell was found to be increased by three-fold (n=3 assays, p<0.01), for the cells plated on particles exposing RGD-peptides when compared to cells on control particles. This indicates a stimulation of the cells due to the exposure of the bioactive RGD-moieties and an enhanced cell-biomaterial interaction. Using nanoparticles is a novel approach to direct cellular behavior with numerous possible applications in tissue engineering such as substrate for dermal and epithelial cells, injectable suspensions or as building blocks to form scaffolds.
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai Ernest Ansermet 30, 1211 Geneva, Switzerland.
authors.forename: Annasara
authors.initials: A
authors.lastname: Hansson
authors.forename: Tiziana
authors.initials: T
authors.lastname: Di Francesco
authors.forename: Françoise
authors.initials: F
authors.lastname: Falson
authors.forename: Patricia
authors.initials: P
authors.lastname: Rousselle
authors.forename: Olivier
authors.initials: O
authors.lastname: Jordan
authors.forename: Gerrit
authors.initials: G
authors.lastname: Borchard
mesh_terms: D002448:Cell Adhesion; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry* / Q000633:toxicity; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D009842:Oligopeptides / Q000737:chemistry* / Q000633:toxicity; D023822:Tissue Engineering; D014945:Wound Healing
chemical_list: D009842:Oligopeptides; D002809:Chondroitin Sulfates; D048271:Chitosan; C049554:glycyl-arginyl-glycyl-aspartyl-serine