title: A RubisCO-like protein links SAM metabolism with isoprenoid biosynthesis.
abstract: Functional assignment of uncharacterized proteins is a challenge in the era of large-scale genome sequencing. Here, we combine in extracto NMR, proteomics and transcriptomics with a newly developed (knock-out) metabolomics platform to determine a potential physiological role for a ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO)-like protein from Rhodospirillum rubrum. Our studies unraveled an unexpected link in bacterial central carbon metabolism between S-adenosylmethionine-dependent polyamine metabolism and isoprenoid biosynthesis and also provide an alternative approach to assign enzyme function at the organismic level.
authors.affiliation: Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Champaign, IL, USA.
authors.forename: Tobias J
authors.initials: TJ
authors.lastname: Erb
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Evans
authors.forename: Kyuil
authors.initials: K
authors.lastname: Cho
authors.forename: Benjamin P
authors.initials: BP
authors.lastname: Warlick
authors.forename: Jaya
authors.initials: J
authors.lastname: Sriram
authors.forename: B McKay
authors.initials: BM
authors.lastname: Wood
authors.forename: Heidi J
authors.initials: HJ
authors.lastname: Imker
authors.forename: Jonathan V
authors.initials: JV
authors.lastname: Sweedler
authors.forename: F Robert
authors.initials: FR
authors.lastname: Tabita
authors.forename: John A
authors.initials: JA
authors.lastname: Gerlt
mesh_terms: D003839:Deoxyadenosines / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics; D012247:Rhodospirillum rubrum / Q000201:enzymology*; D012273:Ribulose-Bisphosphate Carboxylase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000737:chemistry / Q000378:metabolism*; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism*; D013872:Thionucleosides / Q000737:chemistry / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics
chemical_list: D003839:Deoxyadenosines; D011073:Polyamines; D013729:Terpenes; D013872:Thionucleosides; C008500:5'-methylthioadenosine; D012436:S-Adenosylmethionine; D012273:Ribulose-Bisphosphate Carboxylase