title: N-glycoproteomics: mass spectrometry-based glycosylation site annotation.
abstract: Glycosylations are ubiquitous and, in many cases, essential protein modifications. Yet comprehensive and detailed analysis of glycosylations on a proteome-wide scale is a daunting and still unsolved challenge. However, a common workflow has emerged over the last decade for large-scale N-glycosylation site annotation by application of proteomic methodology. Thereby, the qualitative and quantitative assessment of hundreds or thousands of modification sites is enabled. This review presents a short overview about common enrichment techniques and glycosylation site detection for N-glycopeptides, including benefits and challenges of analysis.
authors.affiliation: Department of Bioanalytics, Leibniz-Institut für Analytische Wissenschaften, ISAS eV, D-44227 Dortmund, Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Pasing
authors.forename: Albert
authors.initials: A
authors.lastname: Sickmann
authors.forename: Urs
authors.initials: U
authors.lastname: Lewandrowski
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D040901:Proteomics / Q000379:methods*