title: DNA detection based on fluorogenic nanospheres.
abstract: High and dry fluorescence: Fluorogenic nanospheres (green, see scheme) are conjugated to DNA (pink) and used to detect target DNA (aqua). Upon addition of N-butylmorpholine (droplet), the nanospheres dissolve, releasing fluorophores, and intense blue fluorescence is emitted at the site of DNA hybridization. The separation of DNA hybridization and signal amplification gives high sensitivity (100 zmol) and selectivity.
authors.affiliation: Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing, 210093, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Shu
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
mesh_terms: D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D008856:Microscopy, Fluorescence; D054118:Nanospheres / Q000032:analysis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D004247:DNA