title: Application of faecal metabonomics on an experimental model of tubulointerstitial fibrosis by ultra performance liquid chromatography/high-sensitivity mass spectrometry with MS(E) data collection technique.
abstract: Chronic renal failure (CRF) is a major challenge for the public healthcare problem. A novel UPLC Q-TOF/MS method with MS(E) data collection mode was developed as a very effective biochemical analytical tool for precise identification of important biomarkers in the adenine-induced CRF rats. Nine endogenous metabolites were identified by using metabonomic method combined with multivariate data analysis, the accurate mass, isotopic pattern, MS(E) fragments information and MassLynx i-FIT algorithm. The identified metabolites indicated the perturbations of bile acid and phospholipid metabolism are related to CRF rats. This work shows that metabonomics method is a valuable tool in CRF mechanism study.
authors.affiliation: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, the College of Life Sciences, Northwest University, Xi'an, Shaanxi, PR China.
authors.forename: Ying-Yong
authors.initials: YY
authors.lastname: Zhao
authors.forename: Xian-Long
authors.initials: XL
authors.lastname: Cheng
authors.forename: Feng
authors.initials: F
authors.lastname: Wei
authors.forename: Xu
authors.initials: X
authors.lastname: Bai
authors.forename: Rui-Chao
authors.initials: RC
authors.lastname: Lin
mesh_terms: D000225:Adenine; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004195:Disease Models, Animal; D005243:Feces / Q000737:chemistry*; D005355:Fibrosis / Q000139:chemically induced / Q000175:diagnosis; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000139:chemically induced / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods* / Q000706:statistics & numerical data; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D000225:Adenine