title: [Increase in resistance of A431 cancer cells to TRAIL-induced apoptosis in confluent cultures].
abstract: It was shown that cancer cells acquired resistance to TRAIL-induced apoptosis in confluent cultures. Recombinant protein izTRAIL induced apoptosis of human carcinoma A431 cells in the first hours after cell plating at a concentration of 3-10 ng/ml, while in confluent cultures these cells acquire resistance to protein izTRAIL even at the concentration of 2 mkg/ml. Detachment and suspending of the cells of confluent cultures immediately suppressed the resistance to izTRAIL. The cells of confluent cultures, being resistant to TRAIL-induced apoptosis continue progression through the cell cycle, as evidenced by the DNA cytograms and the Ki67p-GFP reporter system. Thus, the results showed that tumor A431 cells can acquire resistance to TRAIL-induced apoptosis in confluent cultures, while continue progression through the cell cycle, keeping the proliferative potential.
authors.forename: R S
authors.initials: RS
authors.lastname: Fadeev
authors.forename: A V
authors.initials: AV
authors.lastname: Chekanov
authors.forename: N V
authors.initials: NV
authors.lastname: Dolgikh
authors.forename: V S
authors.initials: VS
authors.lastname: Akatov
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D019394:Ki-67 Antigen / Q000032:analysis; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000138:chemical synthesis / Q000235:genetics; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000008:administration & dosage / Q000138:chemical synthesis / Q000235:genetics
chemical_list: D019394:Ki-67 Antigen; D011994:Recombinant Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human