title: Development of foot-and-mouth disease virus (FMDV) serotype O virus-like-particles (VLPs) vaccine and evaluation of its potency.
abstract: Foot-and-mouth disease (FMD) is an economically significant viral disease that rampage dairy and other livestock industries in many countries. The disease is being controlled by the use of an inactivated vaccine. However, a recombinant marker vaccine, which avoids the use of live virus, may be an option for the unambiguous differentiation of infected animals from vaccinated animals. A recombinant baculovirus clone containing P1-2A-3C coding sequences of foot-and-mouth disease virus (FMDV) serotype O(1) Manisa was generated. The FMDV structural proteins along with the 3C protease were expressed in Sf9 cells and the generation of virus like particles (VLP) was studied. The recombinant protein was formulated as vaccine using an oil adjuvant, ISA 206 and potency of the vaccine was tested in cattle. The vaccine had a potency value (PD(50)) of 5.01 and most of the vaccinated animals exhibited neutralizing antibody titers after two immunizations.
authors.affiliation: Research and Development Centre, Indian Immunologicals Limited, Gachibowli, Hyderabad 500032, India.
authors.forename: B
authors.initials: B
authors.lastname: Mohana Subramanian
authors.forename: M
authors.initials: M
authors.lastname: Madhanmohan
authors.forename: Rajan
authors.initials: R
authors.lastname: Sriraman
authors.forename: R V
authors.initials: RV
authors.lastname: Chandrasekhar Reddy
authors.forename: S
authors.initials: S
authors.lastname: Yuvaraj
authors.forename: Kankipati
authors.initials: K
authors.lastname: Manikumar
authors.forename: S
authors.initials: S
authors.lastname: Rajalakshmi
authors.forename: S B
authors.initials: SB
authors.lastname: Nagendrakumar
authors.forename: Samir Kumar
authors.initials: SK
authors.lastname: Rana
authors.forename: V A
authors.initials: VA
authors.lastname: Srinivasan
mesh_terms: D000086042:3C Viral Proteases; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology; D016367:Baculoviridae / Q000235:genetics; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D008297:Male; D009500:Neutralization Tests; D012367:RNA, Viral / Q000032:analysis; D061987:Sf9 Cells; D014611:Vaccination / Q000379:methods; D058425:Vaccines, Virus-Like Particle / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral; D012367:RNA, Viral; D058425:Vaccines, Virus-Like Particle; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases