title: Attenuation of KBrO3-induced renal and hepatic toxicity by cloudy apple juice in rat.
abstract: The aim of the study was to evaluate a protective effect of apple juice on KBrO3-induced oxidative stress in rats. Male Wistar rats were administered apple juice per os, 10 ml/kg b.w. for 28 days. On 27 day of the experiment, some rats were given i.p. a single 125 mg/kg b.w. dose of KBrO3 . Markers of oxidative damage and clinical chemistry parameters were determined. Treatment with apple juice prior to KBrO3 challenge prevented an increase in hepatic and renal microsomal lipid peroxidation by 25 and 44%, respectively, increased the activity of antioxidant enzymes in the liver by 29 - 59% and decreased the plasma content of carbonyl groups by 19%. Aminotransferases activity in plasma was reduced by 19% and 36%, concentrations of plasma bilirubin, cholesterol and creatinine were suppressed by 21%, 16% and 26%, respectively, in rats supplemented with juice before KBrO3 injection. No protective effect of apple juice on nuclear DNA was observed. Supplementation with cloudy apple juice to some extent attenuated oxidative damage induced by KBrO3 in the liver and kidney of rats as evidenced by alterations of certain oxidative stress markers and clinical chemistry parameters.
authors.affiliation: Department of Toxicology, Poznań University of Medical Sciences, Poznań, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kujawska
authors.forename: Ewa
authors.initials: E
authors.lastname: Ignatowicz
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Ewertowska
authors.forename: Teresa
authors.initials: T
authors.lastname: Adamska
authors.forename: Jarosław
authors.initials: J
authors.lastname: Markowski
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Jodynis-Liebert
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001628:Beverages; D015415:Biomarkers / Q000097:blood; D001959:Bromates / Q000009:adverse effects; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D027845:Malus / Q000737:chemistry*; D008862:Microsomes, Liver / Q000187:drug effects; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D001959:Bromates; C019536:potassium bromate