title: A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method.
abstract: A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridization efficiency of each section of sandwich structure. The results showed that both multistep and temperature-controlling hybridization techniques were both especially made to fabricate the sensor for the tendency of internal hybridization of target gene sequences. This strategy provides significantly enhanced hybridization efficiency and sequence specificity of electrochemical detection.
authors.affiliation: Department of Laboratory Medicine, The Fuzhou Second Affiliated Hospital of Xiamen University, Fuzhou, China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Hong
authors.forename: Yinhuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Shaohuang
authors.initials: S
authors.lastname: Weng
authors.forename: Qicai
authors.initials: Q
authors.lastname: Liu
authors.forename: Ailin
authors.initials: A
authors.lastname: Liu
authors.forename: Daoxin
authors.initials: D
authors.lastname: Zheng
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lin
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D055029:Inverted Repeat Sequences / Q000235:genetics*; D008839:Microelectrodes; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000295:instrumentation*
chemical_list: D004247:DNA