title: Surface functionalized gold nanorods: tracking and observing live cell via three optical signals.
abstract: Surface functionalized gold nanorods (GNRs) with three optical signals and the ability to track and observe live cells was investigated. The Cetyltrimethyl Ammonium Bromide (CTAB) was used as the shape-directing agent to develop GNRs with uniform size and aspect ratio. The as-synthesized GNRs can strongly enhance the Raman signal of the attached MGITC on the GNRs. Polyethylene glycol (SH-PEG-COOH) and SH-PEG-FITC were attached with GNRs through ligand exchange with CTAB. They can stabilize the GNRs, reduce the cytotoxicity and provide fluorescence signal. A cell-penetrating peptide was covalently conjugated with SH-PEG-COOH to assist the GNRs to enter living cell. After cultured with the MCF-7 cells, the surfaced functionalized Au nanorods can enter the MCF-7 cells and be tracking with enhanced MGITC Raman signal, FITC fluorescence, and Rayleigh scatter signal.
authors.affiliation: Key Laboratory of Construction and Detection of Guangdong Province, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Xiaozhong
authors.initials: X
authors.lastname: Qiu
authors.forename: Yaling
authors.initials: Y
authors.lastname: Wang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Cui
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Sun
authors.forename: Ru
authors.initials: R
authors.lastname: Liu
authors.forename: Wenhua
authors.initials: W
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Yan
authors.initials: Y
authors.lastname: Zu
authors.forename: Hui
authors.initials: H
authors.lastname: Yuan
authors.forename: Xueling
authors.initials: X
authors.lastname: Chang
authors.forename: Xueyun
authors.initials: X
authors.lastname: Gao