title: Enhanced top-down characterization of histone post-translational modifications.
abstract: Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) platform for high-throughput and sensitive characterization of histone PTMs at the intact protein level. The platform enables unambiguous identification of 708 histone isoforms from a single 2D LC-MS/MS analysis of 7.5 µg purified core histones. The throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Tian
authors.forename: Nikola
authors.initials: N
authors.lastname: Tolić
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Moore
authors.forename: Shawna M
authors.initials: SM
authors.lastname: Hengel
authors.forename: Errol W
authors.initials: EW
authors.lastname: Robinson
authors.forename: David L
authors.initials: DL
authors.lastname: Stenoien
authors.forename: Si
authors.initials: S
authors.lastname: Wu
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Paša-Tolić
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D006367:HeLa Cells; D006657:Histones / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D020033:Protein Isoforms / Q000032:analysis; D011499:Protein Processing, Post-Translational; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D006657:Histones; D020033:Protein Isoforms