title: Novel insight into qualitative standardization of Polygoni avicularis herba (Ph. Eur.).
abstract: Polygonum aviculare L. (Common Knotrgrass) (Polygonaceae Juss.) is an annual from which pharmacopoeial (Ph. Eur.) plant material Polygoni avicularis herba is obtained. Although its main active constituents are flavonoids and its standardization is based on their total content, no profound qualitative analysis has been performed, yet. The use of hyphenated analytical methods: UHPLC-DAD coupled with ion trap or time of flight mass detectors together with acidic hydrolysis products analysis allowed for a comprehensive determination of flavonoid composition. Among dominating compounds, myricetin, kaempferol, isorhamnetin and kaempferide glucuronides, which were not previously reported in P. avicularis herba, were discovered. The developed method can be used as a suitable tool for a more insightful, metabolome-based standardization of flavonoid rich pharmacopoeial plant material -P. avicularis herba.
authors.affiliation: Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Faculty of Pharmacy, Banacha St. 1, 02-097 Warsaw, Poland.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Granica
authors.forename: Jakub P
authors.initials: JP
authors.lastname: Piwowarski
authors.forename: Magdalena
authors.initials: M
authors.lastname: Popławska
authors.forename: Marta
authors.initials: M
authors.lastname: Jakubowska
authors.forename: Joanna
authors.initials: J
authors.lastname: Borzym
authors.forename: Anna K
authors.initials: AK
authors.lastname: Kiss
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000737:chemistry*; D020719:Glucuronides / Q000737:chemistry; D044949:Kaempferols / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry*; D028502:Polygonum / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D005419:Flavonoids; D020719:Glucuronides; D044949:Kaempferols; D010936:Plant Extracts; C047368:3-methylquercetin; C449720:kaempferide; C040015:myricetin; D011794:Quercetin