title: Induced G1 phase arrest of fast-dividing cells improves the quality of genomic profiles generated by array-CGH.
abstract: Genome-wide profiling of copy number alterations by array-based high resolution comparative genomic hybridization (array-CGH) is an important method to ensure the genomic integrity of cells in diverse conditions. We observed that the analysis of genomic profiles, in particular of fast-dividing murine leukemia cell lines, is challenging due to characteristic patterns oscillating around the array-CGH baseline. Here we show array-CGH data can be drastically improved by reducing proliferation rates of cultured cells using deprivation protocols or cell cycle inhibitors. Arresting cell cycle in the G1 phase leads to smoother genomic profiles, and hence to a more reliable detection of copy number alterations.
authors.affiliation: Institute of Cell and Molecular Pathology, Hannover Medical School, Hannover, Germany.
authors.forename: Georgi
authors.initials: G
authors.lastname: Manukjan
authors.forename: Marcel
authors.initials: M
authors.lastname: Tauscher
authors.forename: Tim
authors.initials: T
authors.lastname: Ripperger
authors.forename: Adrian
authors.initials: A
authors.lastname: Schwarzer
authors.forename: Brigitte
authors.initials: B
authors.lastname: Schlegelberger
authors.forename: Doris
authors.initials: D
authors.lastname: Steinemann
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D055028:Comparative Genomic Hybridization; D056915:DNA Copy Number Variations; D059585:G1 Phase Cell Cycle Checkpoints; D020869:Gene Expression Profiling; D005821:Genetic Techniques; D023281:Genomics / Q000592:standards*; D018922:HL-60 Cells; D006801:Humans; D051379:Mice