title: Energy metabolism of rat cerebral cortex, hypothalamus and hypophysis during ageing.
abstract: Ageing is one of the main risk factors for brain disorders. According to the neuroendocrine theory, ageing modifies the sensitivity of hypothalamus-pituitary-adrenal axis to homoeostatic signals coming from the cerebral cortex. The relationships between the energy metabolism of these areas have not been considered yet, in particular with respect to ageing. For these reasons, this study was undertaken to systematically investigate in female Sprague-Dawley rats aged 4, 6, 12, 18, 24, 28 months and in 4-month-old male ones, the catalytic properties of energy-linked enzymes of the Krebs' cycle, electron transport chain, glutamate and related amino acids on different mitochondrial subpopulations, i.e. non-synaptic perikaryal and intra-synaptic (two types) mitochondria. The biochemical enzymatic pattern of these mitochondria shows different expression of the above-mentioned enzymatic activities in the investigated brain areas, including frontal cerebral cortex, hippocampus, striatum, hypothalamus and hypophysis. The study shows that: (i) the energy metabolism of the frontal cerebral cortex is poorly affected by physiological ageing; (ii) the biochemical machinery of non-synaptic perikaryal mitochondria is differently expressed in the considered brain areas; (iii) at 4-6 months, hypothalamus and hypophysis possess lower oxidative metabolism with respect to the frontal cerebral cortex while (iv), during ageing, the opposite situation occurs. We hypothesised that these metabolic modifications likely try to grant HPA functionality in response to the incoming external stress stimuli increased during ageing. It is particularly notable that age-related changes in brain bioenergetics and in mitochondrial functionality may be considered as remarkable factors during physiological ageing and should play important roles in predisposing the brain to physiopathological events, tightly related to molecular mechanisms evoked for pharmacological treatments.
authors.affiliation: University of Pavia, Department of Biology and Biotechnology, Laboratory of Pharmacology and Molecular Medicine of Central Nervous System, Via Ferrata 9, 27100 Pavia, Italy. robertofederico.villa@unipv.it
authors.forename: R F
authors.initials: RF
authors.lastname: Villa
authors.forename: F
authors.initials: F
authors.lastname: Ferrari
authors.forename: A
authors.initials: A
authors.lastname: Gorini
mesh_terms: D000367:Age Factors; D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000502:physiology* / Q000648:ultrastructure; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D007031:Hypothalamus / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D010902:Pituitary Gland / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013574:Synaptosomes / Q000378:metabolism