title: Overexpression of interleukin 2 receptor, thymidine kinase and immunoglobulin-associated alpha-1 messenger RNA in a clinical case of enzootic bovine leukosis.
abstract: A 49-month-old Holstein cow with anorexia, tachypnea, enlarged peripheral lymph nodes, and difficulty standing up was suspected of bovine leukosis. Hematological examination revealed lymphocytosis with the presence of neoplastic cells. Increased total lactate dehydrogenase (LDH) activity, isozymes of LDH-2 and LDH-3 activities and thymidine kinase activity were observed. Cytological findings of fine needle aspiration of subiliac lymph nodes indicated lymphosarcoma. Histopathology and antibody analysis confirmed the diagnosis of enzootic bovine leukosis, a B-cell bovine lymphoma caused by bovine leukemia virus. Gene expressions known as biomarkers of hematopoietic neoplasia in human were also examined in the present case. Increased messenger RNA expression of interleukin 2 receptor, thymidine kinase, and immunoglobulin-associated alpha-1 was observed in the case animal.
authors.affiliation: Department of Clinical Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Mohammad Monir
authors.initials: MM
authors.lastname: Tawfeeq
authors.forename: Michihito
authors.initials: M
authors.lastname: Tagawa
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Itoh
authors.forename: Kazuya
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Yoshiyasu
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000276:immunology* / Q000378:metabolism; D044963:Biopsy, Fine-Needle / Q000662:veterinary; D051925:CD79 Antigens / Q000276:immunology* / Q000378:metabolism; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D016583:Enzootic Bovine Leukosis / Q000276:immunology* / Q000473:pathology; D017809:Fatal Outcome; D005260:Female; D045022:Lactate Dehydrogenases / Q000378:metabolism; D008198:Lymph Nodes / Q000473:pathology*; D012333:RNA, Messenger / Q000276:immunology* / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000276:immunology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013937:Thymidine Kinase / Q000276:immunology* / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D051925:CD79 Antigens; D017931:DNA Primers; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; D045022:Lactate Dehydrogenases; D013937:Thymidine Kinase