title: Conjunctival inflammation in patients under topical glaucoma treatment with indication to surgery.
abstract: OBJECTIVE
  To compare the frequency of conjunctival HLA-DR expression (a surrogate marker for inflammation) in eyes treated with topical prostaglandin analogues versus eyes treated with other topical antiglaucomatous drugs.
  
  
  METHODS
  Patients diagnosed with primary open-angle glaucoma presenting indication for trabeculectomy were divided in groups according to the use or not of prostaglandin analogues. All subjects were treated with the maximum tolerated dose of antiglaucomatous drugs until the date of the surgery. At the beginning of the surgical procedure, a 5 x 5 mm biopsy of the bulbar conjunctiva was collected, incubated with monoclonal anti-HLA-DR antibody and processed for histological analysis.
  
  
  RESULTS
  Of the 31 eyes included (31 patients), 25 were under topical prostaglandin analogues (Group 1) and six under other topical pharmacological agents (Group 2). Fourteen eyes of Group 1 (56%) and three of Group 2 (50 %) were positive for the inflammatory marker HLA-DR (P=1.0). The percentage of stained cells ranged from 15.49 to 48.09% (median: 27.61) in Group 1, and from 18.35 to 28 (median: 20.71) in Group 2, with no differences statistically significant (p=0.33).
  
  
  CONCLUSIONS
  The use of prostaglandin analogues did not increase conjunctival expression of HLA-DR compared to other topical antiglaucomatous agents.
authors.affiliation: FMRP-USP, Brazil.
authors.forename: João Marcello
authors.initials: JM
authors.lastname: Furtado
authors.forename: Jayter Silva
authors.initials: JS
authors.lastname: Paula
authors.forename: Edson Garcia
authors.initials: EG
authors.lastname: Soares
authors.forename: Neifi Hassam Saloum
authors.initials: NH
authors.lastname: Dhegaide
authors.forename: Eduardo Melani
authors.initials: EM
authors.lastname: Rocha
authors.forename: Eduardo
authors.initials: E
authors.lastname: Donadi
authors.forename: Maria de Lourdes Veronese
authors.initials: Mde L
authors.lastname: Rodrigues
mesh_terms: D060433:Administration, Ophthalmic; D000368:Aged; D000704:Analysis of Variance; D015415:Biomarkers / Q000032:analysis; D001706:Biopsy; D003228:Conjunctiva / Q000187:drug effects* / Q000473:pathology; D003231:Conjunctivitis / Q000139:chemically induced* / Q000473:pathology; D005260:Female; D005901:Glaucoma / Q000188:drug therapy* / Q000601:surgery; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011465:Prostaglandins, Synthetic / Q000009:adverse effects* / Q000627:therapeutic use
chemical_list: D015415:Biomarkers; D006684:HLA-DR Antigens; D011465:Prostaglandins, Synthetic