title: FTIR nanobiosensors for Escherichia coli detection.
abstract: Infections due to enterohaemorrhagic E. coli (Escherichia coli) have a low incidence but can have severe and sometimes fatal health consequences, and thus represent some of the most serious diseases due to the contamination of water and food. New, fast and simple devices that monitor these pathogens are necessary to improve the safety of our food supply chain. In this work we report on mesoporous titania thin-film substrates as sensors to detect E. coli O157:H7. Titania films treated with APTES ((3-aminopropyl)triethoxysilane) and GA (glutaraldehyde) were functionalized with specific antibodies and the absorption properties monitored. The film-based biosensors showed a detection limit for E. coli of 1 × 10(2) CFU/mL, constituting a simple and selective method for the effective screening of water samples.
authors.affiliation: Nucleo Ricerca Desertificazione, Università degli Studi di Sassari, Viale Italia 57, 07100 Sassari, Italy ; Department of Agricultural and Biological Engineering and Bindley Bioscience Center, Purdue University, 225 S. University Street, West Lafayette, 47907, Indiana ; Lea Nanotech s.r.l. S.P. 55 Porto Conte/Capo Caccia, km 8.400 località Tramariglio, 07041 Alghero (SS), Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Mura
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Greppi
authors.forename: Maria Laura
authors.initials: ML
authors.lastname: Marongiu
authors.forename: Pier Paolo
authors.initials: PP
authors.lastname: Roggero
authors.forename: Sandeep P
authors.initials: SP
authors.lastname: Ravindranath
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Mauer
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Schibeci
authors.forename: Francesco
authors.initials: F
authors.lastname: Perria
authors.forename: Massimo
authors.initials: M
authors.lastname: Piccinini
authors.forename: Plinio
authors.initials: P
authors.lastname: Innocenzi
authors.forename: Joseph
authors.initials: J
authors.lastname: Irudayaraj