title: Purification, crystallization and preliminary crystallographic analysis of the CBS-domain pair of cyclin M2 (CNNM2).
abstract: This work describes the purification and preliminary crystallographic analysis of the CBS-domain pair of the murine CNNM2 magnesium transporter (formerly known as ancient domain protein 2; ACDP2), which consists of a pair of cystathionine β-synthase (CBS) motifs and has 100% sequence identity to its human homologue. CNNM proteins represent the least-studied members of the eight different types of magnesium transporters identified to date in mammals. In humans, the CNNM family is encoded by four genes: CNNM1-4. CNNM1 acts as a cytosolic copper chaperone, whereas CNNM2 and CNNM4 have been associated with magnesium handling. Interestingly, mutations in the CNNM2 gene cause familial dominant hypomagnesaemia (MIM:607803), a rare human disorder characterized by renal and intestinal magnesium (Mg(2+)) wasting, which may lead to symptoms of Mg(2+) depletion such as tetany, seizures and cardiac arrhythmias. This manuscript describes the preliminary crystallographic analysis of two different crystal habits of a truncated form of the protein containing its regulatory CBS-domain pair, which has been reported to host the pathological mutation T568I in humans. The crystals belonged to space groups P2(1)2(1)2 and I222 (or I2(1)2(1)2(1)) and diffracted X-rays to 2.0 and 3.6 Å resolution, respectively, using synchrotron radiation.
authors.affiliation: Structural Biology Unit, CIC bioGUNE, Parque Tecnológico de Bizkaia, Edificio 800, 48160 Derio, Bizkaia, Spain.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Gómez-García
authors.forename: Marchel
authors.initials: M
authors.lastname: Stuiver
authors.forename: June
authors.initials: J
authors.lastname: Ereño
authors.forename: Iker
authors.initials: I
authors.lastname: Oyenarte
authors.forename: María Angeles
authors.initials: MA
authors.lastname: Corral-Rodríguez
authors.forename: Dominik
authors.initials: D
authors.lastname: Müller
authors.forename: Luis Alfonso
authors.initials: LA
authors.lastname: Martínez-Cruz
mesh_terms: D000818:Animals; D027682:Cation Transport Proteins; D003460:Crystallization; D018360:Crystallography, X-Ray; D016213:Cyclins / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D051379:Mice
chemical_list: C558175:CNNM2 protein, human; D027682:Cation Transport Proteins; D016213:Cyclins