title: Measurement of δ-aminolevulinate dehydratase activity.
abstract: Vertebrate ALAD is a cytosolic enzyme that catalyzes the second step of porphyrin formation, and it is expressed abundantly in liver and erythroid tissues as well as in other tissues. This unit describes a colorimetric assay for measuring ALAD activity in mammalian blood or tissues and the extent of reactivation of the enzyme in lead-inhibited samples.
authors.affiliation: Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Fujita
mesh_terms: D000622:Aminolevulinic Acid / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D057075:Enzyme Assays; D004793:Enzyme Reactivators / Q000494:pharmacology; D006801:Humans; D007855:Lead Poisoning / Q000097:blood / Q000175:diagnosis / Q000201:enzymology; D011162:Porphobilinogen / Q000737:chemistry / Q000378:metabolism; D000623:Porphobilinogen Synthase / Q000097:blood / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D014578:Uroporphyrins / Q000032:analysis / Q000737:chemistry
chemical_list: D004793:Enzyme Reactivators; D014578:Uroporphyrins; D011162:Porphobilinogen; D000622:Aminolevulinic Acid; D000623:Porphobilinogen Synthase