title: Effects of caffeic acid phenethyl ester on anastomotic healing in secondary peritonitis.
abstract: OBJECTIVE
  We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE) on wound healing in left colonic anastomoses in the presence of intraperitoneal sepsis induced by cecal ligation and puncture (CLP) in a rodent model.
  
  
  METHODS
  This experimental study was conducted on 48 male Wistar albino rats. The animals were randomly allocated into four groups and a left colonic anastomosis was performed on the day following sham operation or CLP in all rats: (i) sham-operated control group, laparatomy plus cecal mobilization (n = 12) (Group 1), (ii) sham + CAPE group, identical to Group 1 except for CAPE treatment (10 μmol/kg, intraperitoneally, 30 min before construction of the colonic anastomosis) (n = 12) (Group 2), (iii) CLP group, cecal ligation and puncture (n = 12) (Group 3), and (iv) CLP + CAPE-treated group, 10 μmol/kg, intraperitoneally, 30 min before the construction of colonic anastomosis (n = 12) (Group 4). On the postoperative day 7, the animals were subjected to relaparotomy for in-vivo measurement of the colonic anastomotic bursting pressure. A colonic segment including the anastomotic site was resected for histopathological evaluation and biochemical analyses of hydroxyproline (Hyp) contents, myeloperoxidase (MPO) acivity, malondialdehyde (MDA) levels, reduced glutathione (GSH) levels, and superoxide dismutase (SOD) activity. Body weight changes were examined.
  
  
  RESULTS
  CAPE treatment significantly increased colonic anastomotic bursting pressures (p < .05), colonic anastomotic tissue Hyp contents, and enzymatic and nonenzymatic antioxidant markers (p < .05), and significantly decreased oxidative stress parameters in colonic anastomotic tissues (p < .05). Histopathological scores were significantly better by CAPE administration (p < .05).
  
  
  CONCLUSIONS
  This study clearly showed that CAPE treatment prevented the detrimental effects of intraperitoneal sepsis on colonic anastomotic wound healing. Further clinical studies are required to determine whether CAPE has a useful role in the enhancement of gastrointestinal anastomotic wound healing during particular surgeries in which sepsis-induced organ injury occurs.
authors.affiliation: Department of Gastroenterological Surgery, Turkey Yuksek Ihtisas Teaching and Research Hospital, Ankara, Turkey. zteke md@hotmail.com
authors.forename: Zafer
authors.initials: Z
authors.lastname: Teke
authors.forename: Erdal Birol
authors.initials: EB
authors.lastname: Bostanci
authors.forename: Cigdem
authors.initials: C
authors.lastname: Yenisey
authors.forename: Esra Canan
authors.initials: EC
authors.lastname: Kelten
authors.forename: Suzan
authors.initials: S
authors.lastname: Sacar
authors.forename: Nilufer Genc
authors.initials: NG
authors.lastname: Simsek
authors.forename: Suleyman Ender
authors.initials: SE
authors.lastname: Duzcan
authors.forename: Musa
authors.initials: M
authors.lastname: Akoglu
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D003106:Colon / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D004195:Disease Models, Animal; D005978:Glutathione / Q000378:metabolism; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010538:Peritonitis / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D009195:Peroxidase / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D013482:Superoxide Dismutase / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D002109:Caffeic Acids; D008315:Malondialdehyde; D009195:Peroxidase; D013482:Superoxide Dismutase; C055494:caffeic acid phenethyl ester; D005978:Glutathione; D010626:Phenylethyl Alcohol; D006909:Hydroxyproline