title: Genome-wide mRNA profiling and multiplex quantitative RT-PCR for forensic body fluid identification.
abstract: In forensic science, identifying a tissue where a forensic specimen was originated is one of the principal challenges. Messenger RNA (mRNA) profile clearly reveals tissue-specific gene expression patterns that many attempts have been made to use RNA for forensic tissue identification. To systematically investigate the body-fluid-specific expression of mRNAs and find novel mRNA markers for forensic body fluid identification, we performed DNA microarray experiment with 24 Korean body fluid samples. Shannon entropy and Q-values were calculated for each gene, and 137 body-fluid-specific candidate genes were selected. By applying more stringent criteria, we further selected 28 candidate genes and validated them by RT-PCR and qRT-PCR. As a result, we suggest a novel combination of four body-fluid-specific mRNA makers: PPBP for blood, FDCSP for saliva, MSMB for semen and MSLN for vaginal secretion. Multiplex qRT-PCR assay was designed using the four mRNA markers and DNA/RNA co-extraction method was tested for forensic use. This study will provide a thorough examination of body-fluid-specifically expressed mRNAs, which will enlarge the possibility of practical use of RNA for forensic purpose.
authors.affiliation: Medical Genomics Research Center, KRIBB, Department of Functional Genomics, Republic of Korea.
authors.forename: Seong-Min
authors.initials: SM
authors.lastname: Park
authors.forename: Seong-Yeon
authors.initials: SY
authors.lastname: Park
authors.forename: Jeong-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Tae-Wook
authors.initials: TW
authors.lastname: Kang
authors.forename: Jong-Lyul
authors.initials: JL
authors.lastname: Park
authors.forename: Kwang-Man
authors.initials: KM
authors.lastname: Woo
authors.forename: Jong-Sik
authors.initials: JS
authors.lastname: Kim
authors.forename: Han-Chul
authors.initials: HC
authors.lastname: Lee
authors.forename: Seon-Young
authors.initials: SY
authors.lastname: Kim
authors.forename: Seung-Hwan
authors.initials: SH
authors.lastname: Lee
mesh_terms: D001826:Body Fluids; D053803:Forensic Genetics; D020869:Gene Expression Profiling; D015894:Genome, Human; D006801:Humans; D000090204:Mesothelin; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics*; D056910:Republic of Korea
chemical_list: C000715527:MSLN protein, human; D012333:RNA, Messenger; D000090204:Mesothelin