title: Antioxidant properties and cytotoxic effects on human cancer cell lines of aqueous fermented and lipophilic quince (Cydonia oblonga Mill.) preparations.
abstract: In the course of a screening program on quince phytochemicals, two complex preparations were in the focus of the present study, i.e., a lipophilic quince wax extract (QWE) and an aqueous fermented one (QAFE). While the phytochemical composition has been described earlier, the intention of the current investigation was to complement these data with an extensive antioxidant screening of these preparations including their radical scavenging and reductive power as well as their antilipoperoxidative properties. The Quince Aqueous Fermented Extract (QAFE) effectively scavenged the radical target species exhibiting ID(50) values equal to 68.8 μg/mL towards DPPH· and 73.7 μg/mL towards the anion superoxide radical. Quince wax extract (QWE) was more effective at preventing the formation of thiobarbituric reactive species than QAFE exhibiting an ID(50) value equal to 48.9 μg/mL. Moreover the cytotoxic effects towards human HepG2, A549, and HeLa cell lines were evaluated. The two preparations exerted a different effect on the proliferation of the three tested cell lines. Noteworthy, QAFE was almost always more active than QWE but, sometimes, its effects seemed to be strongly dependent on exposure time. Data obtained demonstrate clearly that both hydrophilic and lipophilic quince preparations are non-toxic and exert health-promoting properties.
authors.affiliation: Laboratorio di Fitochimica, Dipartimento di Scienze della Vita, Seconda Università degli Studi di Napoli, Caserta, Italy. severina.pacifico@unina2.it
authors.forename: Severina
authors.initials: S
authors.lastname: Pacifico
authors.forename: Marialuisa
authors.initials: M
authors.lastname: Gallicchio
authors.forename: Antonio
authors.initials: A
authors.lastname: Fiorentino
authors.forename: Anna
authors.initials: A
authors.lastname: Fischer
authors.forename: Ulrich
authors.initials: U
authors.lastname: Meyer
authors.forename: Florian Conrad
authors.initials: FC
authors.lastname: Stintzing
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005285:Fermentation; D006367:HeLa Cells; D006801:Humans; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D027824:Rosaceae / Q000737:chemistry*; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl