title: Comparison of neuroprotective effects of five major lipophilic diterpenoids from Danshen extract against experimentally induced transient cerebral ischemic damage.
abstract: We observed neuroprotective effects of five major lipophilic diterpenes derived from Danshen (Radix Salvia miltiorrhiza) extract, such as cryptotanshinone (CTs), dihydrotanshinone I (DTsI), tanshinone I (TsI), tanshinone IIA (TsIIA) and tanshinone IIB (TsIIB), in the hippocampal CA1 region (CA1) against transient ischemic damage in gerbils. These diterpenes were administered 30min before ischemia-reperfusion and the animals were sacrificed 4days after ischemia-reperfusion. In the vehicle-treated-group, cresyl violet positive (CV(+)) cells and neuronal nuclei (NeuN)(+) neurons were significantly decreased in the CA1. However, in the TsI- and CTs-treated-ischemia-groups, CV(+) and NeuN(+) neurons were abundant in the CA1. In the other groups, the number of CV(+) and NeuN(+) neurons was less than the TsI- and CTs-treated-ischemia-groups. In addition, gliosis induced by ischemic damage was apparently blocked in the TsI- and CTs-treated-ischemia-groups. These results suggest that TsI and CTs among five major lipophilic diterpenes have strong potentials for neuroprotection against ischemic damage.
authors.affiliation: Division of Analytical Bio-imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon, 200-701, South Korea.
authors.forename: Ok Kyu
authors.initials: OK
authors.lastname: Park
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Joon Ha
authors.initials: JH
authors.lastname: Park
authors.forename: In Hye
authors.initials: IH
authors.lastname: Kim
authors.forename: Bing Chun
authors.initials: BC
authors.lastname: Yan
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ahn
authors.forename: Seung-Hae
authors.initials: SH
authors.lastname: Kwon
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Lee
authors.forename: Young Sup
authors.initials: YS
authors.lastname: Kim
authors.forename: Misook
authors.initials: M
authors.lastname: Kim
authors.forename: Il-Jun
authors.initials: IJ
authors.lastname: Kang
authors.forename: Jong-Dai
authors.initials: JD
authors.lastname: Kim
authors.forename: Yun Lyul
authors.initials: YL
authors.lastname: Lee
authors.forename: Moo-Ho
authors.initials: MH
authors.lastname: Won
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy*; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005786:Gene Expression Regulation; D005849:Gerbillinae; D006624:Hippocampus / Q000166:cytology; D008297:Male; D015394:Molecular Structure; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000517:prevention & control; D027561:Salvia miltiorrhiza
chemical_list: D004224:Diterpenes; D004365:Drugs, Chinese Herbal; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; C028972:dan-shen root extract