title: Crystallization and preliminary crystallographic studies of CbsA, a secretory exoglucanase from Xanthomonas oryzae pv. oryzae.
abstract: The bacterial pathogen Xanthomonas oryzae pv. oryzae causes bacterial leaf blight, a serious disease of rice. The secreted exoglucanase CbsA is an important virulence factor of this pathogen. It belongs to the glycosyl hydrolase 6 family of proteins based on the carbohydrate-active enzyme (CAZY) classification. In this study, CbsA has been overexpressed, purified and crystallized. The crystal diffracted to a resolution of 1.86 Å and belonged to space group P2(1)2(1)2(1). It contained one monomer per asymmetric unit, with a solvent content of 45.8%.
authors.affiliation: Structural Biology Laboratory, CSIR - Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
authors.forename: Sushil
authors.initials: S
authors.lastname: Kumar
authors.forename: Asfarul S
authors.initials: AS
authors.lastname: Haque
authors.forename: Gopaljee
authors.initials: G
authors.lastname: Jha
authors.forename: Ramesh V
authors.initials: RV
authors.lastname: Sonti
authors.forename: Rajan
authors.initials: R
authors.lastname: Sankaranarayanan
mesh_terms: D003460:Crystallization; D018360:Crystallography, X-Ray; D005959:Glucosidases / Q000737:chemistry*; D014974:Xanthomonas / Q000201:enzymology*
chemical_list: D005959:Glucosidases