title: [Optimum extraction technology of total triterpenoids from Hypodematium sinense].
abstract: OBJECTIVE
  To optimize the extraction technology of total triterpenoids from Hypodematium sinense.
  
  
  METHODS
  With 5% vanillin-glacial acetic acid solution and 72% sulfuric acid as chromogenic agent and the content of total tripenoids as index,using single factor experiment and orthogonal test,the optimal extraction condition was determined.
  
  
  RESULTS
  The optimal conditions were solid-liquid ratio 1:12, 60% ethanol concentration, and ultrasonic extraction time of 60 min at 60 degrees C.
  
  
  CONCLUSIONS
  The extraction technology is feasible and can be used as extraction process of total triterpenoids from Hypodematium sinense.
authors.affiliation: School of Pharmacy, Jining Medical University, Rizhao 276826, China. hualizhang@163.com
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Gui-Hua
authors.initials: GH
authors.lastname: Gao
authors.forename: Min-Na
authors.initials: MN
authors.lastname: Zhang
authors.forename: Yan-Lian
authors.initials: YL
authors.lastname: Li
mesh_terms: D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D029624:Ferns / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014315:Triterpenes / Q000032:analysis / Q000302:isolation & purification*; D014465:Ultrasonics
chemical_list: D004365:Drugs, Chinese Herbal; D012997:Solvents; D014315:Triterpenes; D000431:Ethanol