title: Development of 161 novel EST-SSR markers from Lathyrus sativus (Fabaceae).
abstract: OBJECTIVE
  Expressed sequence tag (ESTs)-derived microsatellite markers were developed in Lathyrus sativus by screening the National Center for Biotechnology Information (NCBI) database. The usefulness of these novel markers was validated for size polymorphism among grasspea accessions. •
  
  
  RESULTS
  Three hundred EST-simple sequence repeat (SSR) primer pairs were identified and loci characterized for size polymorphism among 24 grasspea accessions from worldwide sources. Among them 139 SSR loci produced no PCR product, 117 SSR loci were monomorphic, and 44 SSR loci were polymorphic. The mean number of alleles per locus ranged from two to 11. The observed heterozygosity and expected heterozygosity ranged from 0.000 to 1.000 and 0.042 to 0.836, respectively. •
  
  
  CONCLUSIONS
  These novel markers will be useful and convenient to study genetic mapping and molecular breeding in grasspea.
authors.affiliation: Institute of Crop Science, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, People's Republic of China.
authors.forename: Xue-Lian
authors.initials: XL
authors.lastname: Sun
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Guan
authors.forename: Yu
authors.initials: Y
authors.lastname: Ma
authors.forename: Jun-Ye
authors.initials: JY
authors.lastname: Jiang
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.forename: Marina
authors.initials: M
authors.lastname: Burlyaeva
authors.forename: Margarita
authors.initials: M
authors.lastname: Vishnyakova
authors.forename: Elena
authors.initials: E
authors.lastname: Semenova
authors.forename: Sergey
authors.initials: S
authors.lastname: Bulyntsev
authors.forename: Xu-Xiao
authors.initials: XX
authors.lastname: Zong
mesh_terms: D060146:Ecotype; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D027807:Lathyrus / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers