title: SB365, Pulsatilla saponin D suppresses the proliferation of human colon cancer cells and induces apoptosis by modulating the AKT/mTOR signalling pathway.
abstract: Pulsatilla koreana has been used as a traditional medicine for the treatment of several diseases. The purpose of this study was to determine if SB365, Pulsatilla saponin D isolated from the root of P. koreana inhibits the progression of colon cancer. We found that SB365 strongly suppressed the growth and proliferation of colon cancer cells and induced their apoptosis. Also, SB365 showed anti-angiogenic activity by decreasing the expression of HIF-1α and VEGF. These results were confirmed by an in vivo study showing that SB365 significantly inhibited tumor growth by the induction of apoptosis and inhibition of angiogenesis with stronger anticancer activity than 5-FU. When further examined for its anticancer mechanism, SB365 effectively suppressed the AKT/mTOR pathway both in vitro and in vivo. Taken together, our study demonstrated that SB365 inhibits the AKT/mTOR pathway, leading to the suppression of tumor growth and angiogenesis together with induction of apoptosis. Therefore, SB365 is a good candidate as a natural product for use in the treatment of colon cancer.
authors.affiliation: Department of Biomedical Sciences, Inha University, Incheon, Republic of Korea.
authors.forename: Mi Kwon
authors.initials: MK
authors.lastname: Son
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Jung
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Hong
authors.forename: Hee-Seung
authors.initials: HS
authors.lastname: Lee
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Zheng
authors.forename: Myung-Joo
authors.initials: MJ
authors.lastname: Choi
authors.forename: Ju Hyeon
authors.initials: JH
authors.lastname: Seo
authors.forename: Jun-Kyu
authors.initials: JK
authors.lastname: Suh
authors.forename: Soon-Sun
authors.initials: SS
authors.lastname: Hong
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D015536:Down-Regulation / Q000187:drug effects; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D031946:Pulsatilla / Q000737:chemistry*; D012503:Saponins / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D012503:Saponins; C546842:MTOR protein, human; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases