title: BDNF, produced by a TPO-stimulated megakaryocytic cell line, regulates autocrine proliferation.
abstract: While human platelets release endogenous brain-derived neurotrophic factor (BDNF) upon activation, a previous report on MEG-01, a megakaryocytic cell line, found no trace of BDNF production, and the pathophysiological function of platelet BDNF has remained elusive. In the present study, we demonstrate that MEG-01 produces BDNF in the presence of TPO and that this serves to potentiate cell proliferation. Our in vitro findings suggest that BDNF regulates MEG-01 proliferation in an autocrine manner, and we suggest that BDNF may be a physiological autocrine regulator of megakaryocyte progenitors.
authors.affiliation: Graduate School of Health Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Tamura
authors.forename: Ayumi
authors.initials: A
authors.lastname: Nagasawa
authors.forename: Yuya
authors.initials: Y
authors.lastname: Masuda
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tsunematsu
authors.forename: Koji
authors.initials: K
authors.lastname: Hayasaka
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Matsuno
authors.forename: Chikara
authors.initials: C
authors.lastname: Shimizu
authors.forename: Yukio
authors.initials: Y
authors.lastname: Ozaki
authors.forename: Takanori
authors.initials: T
authors.lastname: Moriyama
mesh_terms: D019898:Autocrine Communication / Q000187:drug effects / Q000502:physiology*; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013926:Thrombopoietin / Q000494:pharmacology
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D013926:Thrombopoietin