title: A lysosome-targetable and two-photon fluorescent probe for monitoring endogenous and exogenous nitric oxide in living cells.
abstract: A lysosome-specific and two-photon fluorescent probe, Lyso-NINO, demonstrates high selectivity and sensitivity toward NO, lower cytotoxicity, and perfect lysosomal localization. With the aid of Lyso-NINO, the first capture of NO within lysosomes of macrophage cells has been achieved using both two-photon fluorescence microscopy and flow cytometry.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Yu
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.forename: Liji
authors.initials: L
authors.lastname: Jin
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008247:Lysosomes / Q000737:chemistry*; D061986:MCF-7 Cells; D008264:Macrophages / Q000737:chemistry* / Q000166:cytology; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D009025:Morpholines / Q000737:chemistry* / Q000494:pharmacology; D053644:Naphthalimides / Q000737:chemistry* / Q000494:pharmacology; D009569:Nitric Oxide / Q000032:analysis*; D017785:Photons; D013329:Structure-Activity Relationship
chemical_list: D005456:Fluorescent Dyes; C579248:Lyso-NINO; D009025:Morpholines; D053644:Naphthalimides; D009569:Nitric Oxide