title: Transcriptional analysis of prebiotic uptake and catabolism by Lactobacillus acidophilus NCFM.
abstract: The human gastrointestinal tract can be positively modulated by dietary supplementation of probiotic bacteria in combination with prebiotic carbohydrates. Here differential transcriptomics and functional genomics were used to identify genes in Lactobacillus acidophilus NCFM involved in the uptake and catabolism of 11 potential prebiotic compounds consisting of α- and β-linked galactosides and glucosides. These oligosaccharides induced genes encoding phosphoenolpyruvate-dependent sugar phosphotransferase systems (PTS), galactoside pentose hexuronide (GPH) permease, and ATP-binding cassette (ABC) transporters. PTS systems were upregulated primarily by di- and tri-saccharides such as cellobiose, isomaltose, isomaltulose, panose and gentiobiose, while ABC transporters were upregulated by raffinose, Polydextrose, and stachyose. A single GPH transporter was induced by lactitol and galactooligosaccharides (GOS). The various transporters were associated with a number of glycoside hydrolases from families 1, 2, 4, 13, 32, 36, 42, and 65, involved in the catabolism of various α- and β-linked glucosides and galactosides. Further subfamily specialization was also observed for different PTS-associated GH1 6-phospho-β-glucosidases implicated in the catabolism of gentiobiose and cellobiose. These findings highlight the broad oligosaccharide metabolic repertoire of L. acidophilus NCFM and establish a platform for selection and screening of both probiotic bacteria and prebiotic compounds that may positively influence the gastrointestinal microbiota.
authors.affiliation: Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
authors.forename: Joakim Mark
authors.initials: JM
authors.lastname: Andersen
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Barrangou
authors.forename: Maher Abou
authors.initials: MA
authors.lastname: Hachem
authors.forename: Sampo J
authors.initials: SJ
authors.lastname: Lahtinen
authors.forename: Yong-Jun
authors.initials: YJ
authors.lastname: Goh
authors.forename: Birte
authors.initials: B
authors.lastname: Svensson
authors.forename: Todd R
authors.initials: TR
authors.lastname: Klaenhammer
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D002475:Cellobiose / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005936:Glucans / Q000494:pharmacology; D007534:Isomaltose / Q000031:analogs & derivatives / Q000494:pharmacology; D007779:Lactobacillus acidophilus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D009844:Oligosaccharides / Q000494:pharmacology; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D056692:Prebiotics; D011887:Raffinose / Q000494:pharmacology; D013402:Sugar Alcohols / Q000494:pharmacology
chemical_list: D018528:ATP-Binding Cassette Transporters; D005936:Glucans; D009844:Oligosaccharides; D056692:Prebiotics; D013402:Sugar Alcohols; D002475:Cellobiose; C005695:stachyose; C008763:panose; D007534:Isomaltose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C014635:lactitol; D011887:Raffinose; C008189:isomaltulose; C033375:polydextrose