title: Fluorometric techniques for the detection of nitric oxide and metabolites.
abstract: Recently fluorometric techniques have been developed to determine NO and S-nitrosothiols with improved sensitivity over spectrophotometric techniques based on similar chemistry. This unit contains protocols for detection and quantification of NO from chemical reactions and from cellular systems, and of S-nitrosothiols in chemical, biochemical, and cellular experiments.
authors.affiliation: National Cancer Institute, Bethesda, Maryland, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Wink
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: A
authors.initials: A
authors.lastname: Miles
authors.forename: D
authors.initials: D
authors.lastname: Jourd'heuil
authors.forename: M B
authors.initials: MB
authors.lastname: Grisham
mesh_terms: D000818:Animals; D005470:Fluorometry; D006801:Humans; D007700:Kinetics; D009566:Nitrates / Q000032:analysis / Q000378:metabolism; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism*; D015538:Nitrosation; D026403:S-Nitrosothiols / Q000032:analysis / Q000378:metabolism
chemical_list: D009566:Nitrates; D026403:S-Nitrosothiols; D009569:Nitric Oxide