title: Cell transformation assays.
abstract: Morphological transformation of mammalian cells following exposure to a chemical agent is frequently used as a preliminary screen for the carcinogenic potential of the agent. This unit describes a protocol using Syrian hamster embryo (SHE) cells to assay transformation.
authors.affiliation: New York University School of Medicine, New York, New York, USA.
authors.forename: M
authors.initials: M
authors.lastname: Costa
authors.forename: J E
authors.initials: JE
authors.lastname: Sutherland
mesh_terms: D000818:Animals; D015197:Carcinogenicity Tests / Q000379:methods*; D002273:Carcinogens / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002478:Cells, Cultured; D006224:Cricetinae; D004622:Embryo, Mammalian / Q000166:cytology / Q000187:drug effects*; D005260:Female; D008647:Mesocricetus; D011247:Pregnancy; D015262:Xenobiotics / Q000633:toxicity*
chemical_list: D002273:Carcinogens; D015262:Xenobiotics