title: Development of an enzyme-linked immunosorbent assay for determination of the furaltadone etabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in animal tissues.
abstract: OBJECTIVE
  To determine 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) residues released from protein bound AMOZ in animal tissues.
  
  
  METHODS
  Polyclonal and monoclonal antibodies were produced in this study. A rapid, sensitive, and specific competitive direct enzyme-linked immunosorbent assay (cdELISA) was developed.
  
  
  RESULTS
  Rabbit polyclonal antibodies were used in the optimized cdELISA method, and exhibited negligible cross-reactivity with other compounds structurally related to AMOZ. The IC(50) of the polyclonal antibody was 0.16 ng/mL. The method limit of detection in four different types of animal and fish tissues was less than 0.06 μg/kg. Recoveries ranged from 80% to 120% for fortified samples with the coefficient of variation values less than 15%. The results of the cdELISA method were in good agreement with the results from an established liquid chromatography-tandem mass spectrometry confirmatory method used for AMOZ residues.
  
  
  CONCLUSIONS
  The cdELISA method developed in the present study is a convenient practical tool for screening large numbers of animal and fish tissue samples for the the detection of released protein bound AMOZ residues.
authors.affiliation: Ministry of Health Key Laboratory, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Peng Jie
authors.initials: PJ
authors.lastname: Luo
authors.forename: Wen Xiao
authors.initials: WX
authors.lastname: Jiang
authors.forename: Ross C
authors.initials: RC
authors.lastname: Beier
authors.forename: Jian Zhong
authors.initials: JZ
authors.lastname: Shen
authors.forename: Hai Yang
authors.initials: HY
authors.lastname: Jiang
authors.forename: Hong
authors.initials: H
authors.lastname: Miao
authors.forename: Yun Feng
authors.initials: YF
authors.lastname: Zhao
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Yong Ning
authors.initials: YN
authors.lastname: Wu
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015394:Molecular Structure; D009025:Morpholines / Q000032:analysis* / Q000737:chemistry; D009581:Nitrofurans / Q000032:analysis* / Q000737:chemistry; D023303:Oxazolidinones / Q000032:analysis* / Q000737:chemistry
chemical_list: C504349:3-amino-5-morpholinomethyl-2-oxazolidinone; D009025:Morpholines; D009581:Nitrofurans; D023303:Oxazolidinones; C007795:furaltadon