title: Quantification of the radio-metabolites of the serotonin-1A receptor radioligand [carbonyl-11C]WAY-100635 in human plasma: an HPLC-assay which enables measurement of two patients in parallel.
abstract: [Carbonyl-(11)C]WAY-100635 is a potent and effective antagonist for the 5-HT(1A) receptor subtype. We aimed to assess the status of [carbonyl-(11)C]WAY-100635 and its main radio-metabolites, [carbonyl-(11)C]desmethyl-WAY-100635 and [carbonyl-(11)C]cyclohexanecarboxylic acid, on the basis of an improved radio-HPLC method. Common methods were characterized by preparative HPLC columns with long runtimes and/or high flow rates. Considering the short half-life of C-11, we developed a more rapid and solvent saving HPLC assay, allowing a fast, efficient and reliable quantification of these major metabolites.
authors.affiliation: Department of Nuclear Medicine, Medical University of Vienna, Austria.
authors.forename: L
authors.initials: L
authors.lastname: Nics
authors.forename: A
authors.initials: A
authors.lastname: Hahn
authors.forename: M
authors.initials: M
authors.lastname: Zeilinger
authors.forename: C
authors.initials: C
authors.lastname: Vraka
authors.forename: J
authors.initials: J
authors.lastname: Ungersboeck
authors.forename: D
authors.initials: D
authors.lastname: Haeusler
authors.forename: S
authors.initials: S
authors.lastname: Hartmann
authors.forename: K-H
authors.initials: KH
authors.lastname: Wagner
authors.forename: R
authors.initials: R
authors.lastname: Lanzenberger
authors.forename: W
authors.initials: W
authors.lastname: Wadsak
authors.forename: M
authors.initials: M
authors.lastname: Mitterhauser
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D010879:Piperazines / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D011725:Pyridines / Q000097:blood / Q000302:isolation & purification / Q000378:metabolism*; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism*; D058829:Serotonin 5-HT1 Receptor Antagonists / Q000378:metabolism
chemical_list: D010879:Piperazines; D011725:Pyridines; D058829:Serotonin 5-HT1 Receptor Antagonists; C112544:desmethyl-WAY 100635; D044282:Receptor, Serotonin, 5-HT1A; C090413:N-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridinyl)cyclohexanecarboxamide