title: Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women.
abstract: The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. Probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected and the FISH technique identified the presence and numbers of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia and Prevotella nigrescens. The Mann-Whitney U-test was applied to compare the data between the two groups. The mean age, ethnicity, marital status, education, and economic level in both groups were similar. The clinical parameters showed no significant differences between pregnant and non-pregnant women. The numbers of subgingival periodontopathogens were not found to be significantly different between groups, despite the higher mean counts of P. intermedia in pregnant women. Colonization patterns of the different bacteria most commonly associated with periodontal disease were not different in the subgingival plaque of pregnant and non-pregnant women.
authors.affiliation: School of Medicine, Univ Federal de Juiz de Fora, MG, Brazil. fercampo@terra.com.br
authors.forename: Fernanda Campos
authors.initials: FC
authors.lastname: Machado
authors.forename: Dionéia Evangelista
authors.initials: DE
authors.lastname: Cesar
authors.forename: Amanda Vervloet Dutra Agostinho
authors.initials: AV
authors.lastname: Assis
authors.forename: Cláudio Galuppo
authors.initials: CG
authors.lastname: Diniz
authors.forename: Rosangela Almeida
authors.initials: RA
authors.lastname: Ribeiro
mesh_terms: D000328:Adult; D001419:Bacteria / Q000254:growth & development*; D058491:Bacterial Load / Q000379:methods*; D018441:Biofilms / Q000254:growth & development*; D003430:Cross-Sectional Studies; D003753:Dental Health Surveys; D005260:Female; D005881:Gingiva / Q000382:microbiology*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D010510:Periodontal Diseases / Q000382:microbiology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000382:microbiology; D018709:Statistics, Nonparametric; D055815:Young Adult