title: Spatially resolved investigation of the oil composition in single intact hyphae of Mortierella spp. with micro-Raman spectroscopy.
abstract: Zygomycetes are well known for their ability to produce various secondary metabolites. Fungi of the genus Mortierella can accumulate highly unsaturated lipids in large amounts as lipid droplets. However, no information about the spatial distribution or homogeneity of the oil inside the fungi is obtainable to date due to the invasive and destructive analytical techniques applied so far. Raman spectroscopy has been demonstrated to be well suited to investigate biological samples on a micrometre scale. It also has been shown that the degree of unsaturation of lipids can be determined from Raman spectra. We applied micro-Raman spectroscopy to investigate the spatial distribution and composition of lipid vesicles inside intact hyphae. For Mortierella alpina and Mortierella elongata distinct differences in the degree of unsaturation and even the impact of growth conditions are determined from the Raman spectra. In both species we found that the fatty acid saturation in the vesicles is highly variable in the first 600 μm of the growing hyphal tip and fluctuates towards a constant composition and saturation ratio in all of the remaining mycelium. Our approach facilitates in vivo monitoring of the lipid production and allows us to investigate the impact of cultivation parameters on the oil composition directly in the growing hyphae without the need for extensive extraction procedures.
authors.affiliation: Institute of Physical Chemistry, Friedrich Schiller University Jena, Helmholtzweg 4, 07743 Jena, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Münchberg
authors.forename: Lysett
authors.initials: L
authors.lastname: Wagner
authors.forename: Eike T
authors.initials: ET
authors.lastname: Spielberg
authors.forename: Kerstin
authors.initials: K
authors.lastname: Voigt
authors.forename: Petra
authors.initials: P
authors.lastname: Rösch
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
mesh_terms: D025301:Hyphae / Q000378:metabolism*; D020094:Mortierella / Q000378:metabolism*; D009821:Oils / Q000378:metabolism*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D009821:Oils