title: Elucidating role of salivary proteins in denture stomatitis using a proteomic approach.
abstract: Denture stomatitis (DS) is the most common oral pathology among denture wearers, affecting over one-third of this group. DS is usually associated with C. albicans. However, unlike other oral candidiasis, most DS patients have intact host immunity. The presence of a denture alone is usually sufficient for DS. Saliva and its protein contents can theoretically predispose some denture wearers to DS and others resistant toward DS. Here we proposed for the first time to define salivary proteomic profiles of denture wearers with and without DS. SELDI-TOF/MS analysis suggests that there is a proteomic differentiation among control, localized and generalized DS. Based on initial SELDI-TOF/MS profiling, we further used reversed phase liquid chromatography, MALDI-TOF/MS, and LC-MS/MS to characterize the salivary proteins associated with DS. Nineteen proteins based on SELDI-TOF/MS profiling were found including cystatin-SN, statherin, kininogen-1, desmocollin-2, carbonic anhydrase-6, peptidyl-prolyl cis-trans isomerase A like peptides, cystatin C, and several immunoglobulin fragments. The proteomic content gives evidence of the interaction between host tissue, saliva, and candida. Further examination in larger populations of these proteins may help to gain a better understanding of DS pathological processes and improve DS treatments.
authors.affiliation: Department of Prosthodontics, School of Dentistry, University of North Carolina, CB#7450, Chapel Hill, NC 27599, USA. Sompop_Bencharit@dentistry.unc.edu
authors.forename: Sompop
authors.initials: S
authors.lastname: Bencharit
authors.forename: Sandra K
authors.initials: SK
authors.lastname: Altarawneh
authors.forename: Sarah Schwartz
authors.initials: SS
authors.lastname: Baxter
authors.forename: Jim
authors.initials: J
authors.lastname: Carlson
authors.forename: Gary F
authors.initials: GF
authors.lastname: Ross
authors.forename: Michael B
authors.initials: MB
authors.lastname: Border
authors.forename: C Russell
authors.initials: CR
authors.lastname: Mack
authors.forename: Warren C
authors.initials: WC
authors.lastname: Byrd
authors.forename: Christopher F
authors.initials: CF
authors.lastname: Dibble
authors.forename: Silvana
authors.initials: S
authors.lastname: Barros
authors.forename: Zvi
authors.initials: Z
authors.lastname: Loewy
authors.forename: Steven
authors.initials: S
authors.lastname: Offenbacher
mesh_terms: D000368:Aged; D002176:Candida albicans / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity; D002180:Candidiasis, Oral / Q000276:immunology / Q000378:metabolism; D003778:Dentures / Q000009:adverse effects*; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D040901:Proteomics; D012463:Saliva / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013282:Stomatitis, Denture / Q000209:etiology* / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology
chemical_list: D007136:Immunoglobulins; D012471:Salivary Proteins and Peptides