title: Micropatterned thermoresponsive surfaces by polymerization of monomer crystals: modulating cellular morphology and cell-substrate interactions.
abstract: A novel and facile approach has been developed to create thermoresponsive surfaces with macroscale patterns together with microscale features. The surface patterns were formed by applying macroscale nucleation agent patterns onto saturated N-isopropylacrylamide monomer solution membranes to induce the divergent growth of needlelike monomer crystals; the patterned monomer crystals were then photopolymerized to form patterned thermoresponsive films. A series of analytical tools (i.e., scanning electron microscopy, profilometry, and contact angle measurement) were used to characterize the properties of the patterned films. Cell coculture on this patterned thermoresponsive films enables cell separation and sorting by modulating temperature- and topography-dependent cell-substrate interactions and cell morphology, respectively. This versatile technique allows the formation of various macroscale patterns with microscale features over large areas, and on most solid substrates, within minutes, all of this without the need for expensive equipment and facilities. Such patterned surfaces can act as both in vitro tumor models and separation platforms for cancer studies. This method can also be applied to other cell-based biological studies and clinical applications.
authors.affiliation: NSF Nanoscale Science and Engineering Center for Affordable Nanoengineering of Polymeric Biomedical Devices, The Ohio State University, Columbus, Ohio 43212, United States.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Hongyan
authors.initials: H
authors.lastname: He
authors.forename: Xinmei
authors.initials: X
authors.lastname: Wang
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Li
authors.forename: Daniel
authors.initials: D
authors.lastname: Gallego-Perez
authors.forename: Jianjun
authors.initials: J
authors.lastname: Guan
authors.forename: L James
authors.initials: LJ
authors.lastname: Lee
mesh_terms: D000178:Acrylamides / Q000737:chemistry* / Q000494:pharmacology*; D000180:Acrylic Resins; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D055616:Microtechnology / Q000379:methods*; D055668:Photochemical Processes; D058105:Polymerization; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D013499:Surface Properties; D013696:Temperature
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D011108:Polymers; C052970:poly-N-isopropylacrylamide