title: Genome-wide association studies-derived susceptibility loci in type 2 diabetes: confirmation in a Chinese population.
abstract: OBJECTIVE
  Several novel genetic variants for type 2 diabetes mellitus (T2DM) have been identified through genome-wide association studies (GWAS). A case-controll study was performed to investigate the association of five new European or South Asian GWAS-derived susceptibility loci with T2DM in a Chinese population.
  
  
  METHODS
  Five single nucleotide polymorphisms (SNPs) were genotyped: rs3923113 near GRB14, rs16861329 in ST6GAL1, rs1802295 in VPS26A, rs7178572 in HMG20A, and rs231362 near KCNQ1, by high-resolution melting (HRM) of small amplicons. The association between T2DM and related quantitative traits in a total of 900 Chinese individuals, including 498 type 2 diabetic patients and 402 ethnically matched control subjects, were examined.
  
  
  RESULTS
  After adjusting for age and gender, rs1802295 (OR 5.724, P=0.03) and rs231362 (OR=5.683, P=0.016) were found to be associated with T2DM. Triglyceride levels were higher in TT and CT carriers for rs16861329 (1.05 (0.8-1.34) mmol/l) than in CC carriers (0.91 (0.73-1.23) mmol/l) with P=0.008 and that high-density lipoprotein cholesterol (HDL-C) was lower in TT and CT carriers (1.17 (1.02-1.33) mmol/l) than in CC carriers (1.21 (1.05-1.41) mmol/l), with P=0.034. For rs3923113, the HDL-C levels were lower in the GG carriers (1.08 (0.90-1.18) mmol/l) than in the GT+TT carriers (1.21 (1.04-1.38) mmol/l), with P=0.018. Ours is the first report of this association.
  
  
  CONCLUSIONS
  rs231362-KCNQ1 and rs1802295-VPS26A are associated with T2DM in the Chinese population. The remaining three SNPs are associated with other aspects of lipid metabolism.
authors.affiliation: Center for Gene Diagnosis Zhongnan Hospital of Wuhan University Donghu Road 169, Wuhan 430071, PR China.
authors.forename: Shuangyan
authors.initials: S
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Xie
authors.forename: Kun
authors.initials: K
authors.lastname: Lin
authors.forename: Siwei
authors.initials: S
authors.lastname: Li
authors.forename: Yidan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Pei
authors.initials: P
authors.lastname: Ma
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Lv
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D008076:Cholesterol, HDL / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D056426:Genetic Loci / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D055106:Genome-Wide Association Study; D006579:Heterozygote; D006801:Humans; D051657:KCNQ1 Potassium Channel / Q000235:genetics; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D014280:Triglycerides / Q000097:blood; D033921:Vesicular Transport Proteins / Q000235:genetics
chemical_list: D008076:Cholesterol, HDL; D051657:KCNQ1 Potassium Channel; C496909:KCNQ1 protein, human; D014280:Triglycerides; C498296:VPS26A protein, human; D033921:Vesicular Transport Proteins