title: [Effect of AKR7A1 overexpression on crotonaldehyde-induced mutagenicity in V79-4 cells].
abstract: OBJECTIVE
  To investigate the effect of overexpressing rat aldo-keto reductases (AKR7A1) in V79-4 cells on crotonaldehyde-induced mutagenicity.
  
  
  METHODS
  The expression level and enzyme activity of AKR7A1 protein expressed in V79-4 cells were measured by Western blotting and AKR enzyme assay,  respectively. HGPRT assay was applied to evaluate the effect of AKR7A1 overexpression on crotonaldehyde-induced mutagenicity in V79-4 cells.
  
  
  RESULTS
  Western blotting   confirmed a high expression level of AKR7A1 protein in V79-4 cells. AKR enzyme assay showed that the overexpressed AKR7A1 protein was functionally active. HGPRT assay demonstrated that cells overexpressing AKR7A1 exhibited a significant increase in resistance to crotonaldehyde-induced mutagenicity compared to control cells.
  
  
  CONCLUSIONS
  Overexpression of AKR7A1 can protect V79-4 cells against crotonaldehyde-induced mutagenicity.
authors.affiliation: School of Pharmaceutical Sciences, China Medical University, Shenyang, China. danlicmu@hotmail.com
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Qi-shan
authors.initials: QS
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Chu
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics* / Q000378:metabolism; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation / Q000187:drug effects* / Q000235:genetics*; D051381:Rats
chemical_list: D000447:Aldehydes; D009153:Mutagens; C012796:2-butenal; C442302:AKR7A1; D000449:Aldehyde Reductase; D007041:Hypoxanthine Phosphoribosyltransferase