title: Effects of SASH1 on melanoma cell proliferation and apoptosis in vitro.
abstract: The SAM and SH3 domain containing 1 (SASH1) gene was originally identified             as a potential tumor suppressor gene in breast cancer, mapped on chromosome 6q24.3.             The expression of SASH1 plays a prognostic role in human colon cancer. Its expression             is frequently downregulated in several human malignancies. However, the biological             function of SASH1 in melanoma cells is yet to be determined. In this study, in             order to investigate the tumor suppressive effects of the SASH1 gene, an A-375             stable melanoma cell line was established, overexpressing the SASH1 gene. The             stable cell line was examined using proliferation assay, apoptosis assay, cell             cycle analysis and real-time PCR. The results indicated that the tumor suppressive             activity of SASH1 derived from G2/M arrest in A-375 cells, and that the phosphorylation             of Cdc2 or the disruption of cyclin B-Cdc2 binding may be responsible for the             G2/M arrest.
authors.affiliation: Children's Hospital, Institute of Biomedical Sciences, Fudan University, Shanghai 200032, P.R. China.
authors.forename: Sheyu
authors.initials: S
authors.lastname: Lin
authors.forename: Junyu
authors.initials: J
authors.lastname: Zhang
authors.forename: Jiawei
authors.initials: J
authors.lastname: Xu
authors.forename: Honglian
authors.initials: H
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Sang
authors.forename: Qinghe
authors.initials: Q
authors.lastname: Xing
authors.forename: Lin
authors.initials: L
authors.lastname: He
mesh_terms: D017209:Apoptosis; D016203:CDC2 Protein Kinase; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019926:Cyclin B / Q000378:metabolism; D018844:Cyclin-Dependent Kinases; D059565:G2 Phase Cell Cycle Checkpoints; D006801:Humans; D059566:M Phase Cell Cycle Checkpoints; D008545:Melanoma / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation; D011485:Protein Binding; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D019926:Cyclin B; C474743:SASH1 protein, human; D025521:Tumor Suppressor Proteins; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases