title: Suppressed increase in blood endothelial progenitor cell content as result of single exhaustive exercise bout in male revascularised coronary artery disease patients.
abstract: Endothelial progenitor cells (EPCs) significantly affect endothelial repair capacity and, hence, cardiovascular disease incidence. In healthy subjects, blood EPC content increases significantly as result of a single maximal exercise test, hereby stimulating endothelial repair capacity. It remains to be shown whether a single exercise positively affects blood EPCs in revascularised coronary artery disease (CAD) patients. From male revascularised CAD patients (n = 60) and healthy volunteers (n = 25) blood samples were collected before and immediately after a maximal cardiopulmonary exercise test. Blood samples were analyzed by optimised flow cytometry methodology for EPC content (CD34+, CD34+ CD133+, CD34+VEGFR2+, CD34+CD133+VEGFR2+, and CD34+CD133-VEGFR2+ cells) and compared between groups. CFU-Hill colonies were additionally assessed. As a result of a maximal exercise test, blood CD34+, CD34+VEGFR2+ (all EPCs), CD34+CD133+, and CD34+ CD133-VEGFR2+ (mature EPCs) cells increased significantly in CAD patients (p < 0.05), but less than in healthy subjects (p < 0.05, and p = 0.06 for CD34+VEGFR2+). CD34+CD133+VEGFR2+ cells (immature EPCs) did not change as result of exercise (p > 0.05). No changes in CFU-Hill colonies as result of exercise were observed. This study shows that blood mature EPCs (CD34+CD133-VEGFR2+) increase significantly as result of a single exercise bout in revascularised CAD patients, but with smaller magnitude compared to healthy subjects. Blood immature EPCs (CD34+CD133+VEGFR2+) did not change significantly as result of exercise.
authors.affiliation: Hasselt University, Faculty of Medicine, Hasselt, Belgium.
authors.forename: J L
authors.initials: JL
authors.lastname: Rummens
authors.forename: A
authors.initials: A
authors.lastname: Daniëls
authors.forename: P
authors.initials: P
authors.lastname: Dendale
authors.forename: K
authors.initials: K
authors.lastname: Hensen
authors.forename: M
authors.initials: M
authors.lastname: Hendrikx
authors.forename: J
authors.initials: J
authors.lastname: Berger
authors.forename: R
authors.initials: R
authors.lastname: Koninckx
authors.forename: D
authors.initials: D
authors.lastname: Hansen
mesh_terms: D015906:Angioplasty, Balloon, Coronary; D015703:Antigens, CD / Q000097:blood; D001026:Coronary Artery Bypass; D003324:Coronary Artery Disease / Q000097:blood / Q000628:therapy*; D042783:Endothelial Cells / Q000166:cytology*; D015444:Exercise; D006801:Humans; D008297:Male; D008875:Middle Aged; D013234:Stem Cells / Q000166:cytology*
chemical_list: D015703:Antigens, CD