title: Methods for measuring DNA adducts and abasic sites II: methods for measurement of DNA adducts.
abstract: This unit contains protocols for analyzing DNA adducts separated from the DNA backbone. HPLC is used to quantify total guanine or ribo- or deoxynucleotides as well as methods for analyzing specific adducts. These methods include HPLC with electrochemical detection, immunoaffininty chromatography to enrich for specific adducts, and gas and liquid chromatography in combination with HPLC and mass spectrometry.
authors.affiliation: University of North Carolina, Chapel Hill, North Carolina.
authors.forename: James A
authors.initials: JA
authors.lastname: Swenberg
authors.forename: Amy-Joan L
authors.initials: AJ
authors.lastname: Ham
authors.forename: Hasan
authors.initials: H
authors.lastname: Koc
authors.forename: David K
authors.initials: DK
authors.lastname: La
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Morinello
authors.forename: Brian F
authors.initials: BF
authors.lastname: Pachkowski
authors.forename: Asoka
authors.initials: A
authors.lastname: Ranasinghe
authors.forename: Patricia B
authors.initials: PB
authors.lastname: Upton
mesh_terms: D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000737:chemistry*; D018736:DNA Adducts / Q000032:analysis*; D055664:Electrochemical Techniques; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D018736:DNA Adducts; D004247:DNA