title: Heterologous production of the lantibiotic Ala(0)actagardine in Escherichia coli.
abstract: We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro.
authors.affiliation: Department of Chemistry and Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, 600 S Mathews Ave, Urbana, IL 61801, USA.
authors.forename: Yanxiang
authors.initials: Y
authors.lastname: Shi
authors.forename: Alejandro
authors.initials: A
authors.lastname: Bueno
authors.forename: Wilfred A
authors.initials: WA
authors.lastname: van der Donk
mesh_terms: D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism; D008969:Molecular Sequence Data; D010455:Peptides; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001430:Bacteriocins; D010455:Peptides; C012993:gardimycin