title: [Analysis of expression pattern of zebrafish leg1 homologus gene mu-leg1 in mouse].
abstract: Gene leg1 (liver-enriched gene 1) was first identified as a novel gene whose expression was enriched in the liver of zebrafish. Further studies revealed that Leg1 protein was a novel secretory protein, which played a role in the liver development in zebrafish. Here we reported the analysis of expression pattern of zb-leg1 homologus gene mu-leg1. The cDNA of mu-leg1 was isolated from adult mouse liver by nested PCR. This gene encodes a putative protein, mu-Leg1, which shares 31% similarity with zb-Leg1 of zebrafish. Both Northern blotting and semi-quantitative RT-PCR demonstrated that the expression of mu-leg1 was enriched in the small intestine rather than in the liver in adult mouse. We also produced a recombined mu-Leg1 protein and a mu-Leg1 specific antibody. Western blottingdemonstrated that mu-Leg1 was a secretory protein. In addition, we have established a mu-leg1 conditional knock-out heterozygous mouse. Our work builds a basis for further studies of mu-leg1.
authors.affiliation: College of Animal Sciences, ZhejiangUniversity, Hangzhou 310058, China. zhuzhihui528@126.com
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Zhu
authors.forename: Min-Jie
authors.initials: MJ
authors.lastname: Hu
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Chang
authors.forename: Jin-Rong
authors.initials: JR
authors.lastname: Peng
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D023061:Gene Order; D018390:Gene Targeting; D005822:Genetic Vectors; D006579:Heterozygote; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D018076:DNA, Complementary; C576822:Liver enriched gene 1 protein, mouse; D011506:Proteins; D011993:Recombinant Fusion Proteins