title: The impact of peptidase activity on clear cell renal cell carcinoma survival.
abstract: Several studies have proposed that protease expression and activity may have a predictive value in the survival of clear cell renal cell carcinoma (CCRCC). Most efforts on this issue have been focused on the analysis of matrix metalloproteinases (MMP) and very little on the role of other proteases, such as peptidases. The catalytic activity of 9 peptidases (APN, APB, ASP, CAP, DPP-IV, NEP/CD10, PEP, PGI, and PSA) was quantified by fluorometric methods in a series of 79 CCRCC patients, and the results obtained were analyzed for survival (Kaplan-Meier curves, log-rank test, and Cox multivariate analysis). CCRCC patients with higher activity levels of membrane-bound APN and soluble APN, DPP-IV, and CAP had significantly shorter 5-yr survival rates than those with lower levels. By contrast, higher soluble APB activity significantly correlated with longer survival. Our data suggest the involvement of peptidases in the biological aggressiveness of CCRCC and support the usefulness of measuring these proteases to assess the prognosis of patients with CCRCC.
authors.affiliation: Dept. of Pathology, Hospital Universitario Cruces, Plaza de Cruces s/n, Bizkaia, Spain.
authors.forename: Gorka
authors.initials: G
authors.lastname: Larrinaga
authors.forename: Lorena
authors.initials: L
authors.lastname: Blanco
authors.forename: Begoña
authors.initials: B
authors.lastname: Sanz
authors.forename: Itxaro
authors.initials: I
authors.lastname: Perez
authors.forename: Javier
authors.initials: J
authors.lastname: Gil
authors.forename: Miguel
authors.initials: M
authors.lastname: Unda
authors.forename: Leire
authors.initials: L
authors.lastname: Andrés
authors.forename: Luis
authors.initials: L
authors.lastname: Casis
authors.forename: José I
authors.initials: JI
authors.lastname: López
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D018826:CD13 Antigens / Q000378:metabolism; D002292:Carcinoma, Renal Cell / Q000201:enzymology* / Q000401:mortality* / Q000473:pathology; D010122:Cystinyl Aminopeptidase / Q000378:metabolism; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D053208:Kaplan-Meier Estimate; D007668:Kidney / Q000201:enzymology* / Q000473:pathology; D007680:Kidney Neoplasms / Q000201:enzymology* / Q000401:mortality* / Q000473:pathology; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D010447:Peptide Hydrolases / Q000378:metabolism*; D011379:Prognosis; D012189:Retrospective Studies; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D010447:Peptide Hydrolases; D018826:CD13 Antigens; D010122:Cystinyl Aminopeptidase; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4