title: Genome-wide technology for determining RNA stability in mammalian cells: historical perspective and recent advantages based on modified nucleotide labeling.
abstract: Changing the abundance of transcripts by regulated RNA degradation is a critical step in the control of various biological pathways. Recently, genome-wide inhibitor-free technologies for determining RNA stabilities in mammalian cells have been developed. In these methods, endogenous RNAs are pulse labeled by uridine analogs [e.g., 4-thiouridine (4sU), 5-etyniluridine (EU) and 5'-bromo-uridine (BrU)], followed by purification of labeled de novo RNAs. These technologies have revealed that the specific half-life of each mRNA is closely related to its physiological function. Genes with short-lived mRNAs are significantly enriched among regulatory genes, while genes with long-lived mRNAs are enriched among housekeeping genes. This review describes the recent progress of experimental procedures for measuring RNA stability.
authors.affiliation: Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Tani
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Akimitsu
mesh_terms: D053959:Alpha-Amanitin / Q000494:pharmacology; D000818:Animals; D001976:Bromouracil / Q000031:analogs & derivatives; D003609:Dactinomycin / Q000494:pharmacology; D005057:Eukaryotic Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020043:Genes, Essential; D005809:Genes, Regulator; D006207:Half-Life; D006801:Humans; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D020871:RNA Stability; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D013891:Thiouridine / Q000032:analysis / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*; D014498:Uracil / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism
chemical_list: D053959:Alpha-Amanitin; D019384:Nucleic Acid Synthesis Inhibitors; D012333:RNA, Messenger; D013891:Thiouridine; D003609:Dactinomycin; C073482:eniluracil; D001976:Bromouracil; D014498:Uracil; C006824:5-bromouridine; D014529:Uridine