title: Isolation and characterization of multipotential mesenchymal cells from the mouse synovium.
abstract: The human synovium contains mesenchymal stem cells (MSCs), which are multipotential non-hematopoietic progenitor cells that can differentiate into a variety of mesenchymal lineages and they may therefore be a candidate cell source for tissue repair. However, the molecular mechanisms by which this can occur are still largely unknown. Mouse primary cell culture enables us to investigate the molecular mechanisms underlying various phenomena because it allows for relatively easy gene manipulation, which is indispensable for the molecular analysis. However, mouse synovial mesenchymal cells (SMCs) have not been established, although rabbit, cow, and rat SMCs are available, in addition to human MSCs. The aim of this study was to establish methods to harvest the synovium and to isolate and culture primary SMCs from mice. As the mouse SMCs were not able to be harvested and isolated using the same protocol for human, rat and rabbit SMCs, the protocol for humans was modified for SMCs from the Balb/c mouse knee joint. The mouse SMCs obtained showed superior proliferative potential, growth kinetics and colony formation compared to cells derived from muscle and bone marrow. They expressed PDGFRá and Sca-1 detected by flow cytometry, and showed an osteogenic, adipogenic and chondrogenic potential similar or superior to the cells derived from muscle and bone marrow by demonstrating in vitro osteogenesis, adipogenesis and chondrogenesis. In conclusion, we established a primary mouse synovial cell culture method. The cells derived from the mouse synovium demonstrated both the ability to proliferate and multipotentiality similar or superior to the cells derived from muscle and bone marrow.
authors.affiliation: Department of Medicine for Motor Organ, Juntendo University Graduate School of Medicine, Tokyo, Japan.
authors.forename: Ippei
authors.initials: I
authors.lastname: Futami
authors.forename: Muneaki
authors.initials: M
authors.lastname: Ishijima
authors.forename: Haruka
authors.initials: H
authors.lastname: Kaneko
authors.forename: Kunikazu
authors.initials: K
authors.lastname: Tsuji
authors.forename: Naoki
authors.initials: N
authors.lastname: Ichikawa-Tomikawa
authors.forename: Ryo
authors.initials: R
authors.lastname: Sadatsuki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Muneta
authors.forename: Eri
authors.initials: E
authors.lastname: Arikawa-Hirasawa
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sekiya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kaneko
mesh_terms: D050156:Adipogenesis / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation / Q000379:methods; D020219:Chondrogenesis / Q000502:physiology; D005260:Female; D016130:Immunophenotyping; D007719:Knee Joint / Q000033:anatomy & histology / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis / Q000502:physiology; D061251:Primary Cell Culture; D013583:Synovial Membrane / Q000166:cytology*