title: Purification of cytochrome P-450 enzymes.
abstract: Among the liver P-450 xenobiotic-metabolizing enzymes, P450-2E1 is of interest because of its activation of potent carcinogens, and P-450 1A2 is of interest because of its role in oxidation of drugs and carcinogens. This unit describes column chromatography protocols for purification of recombinant forms of these enzymes expressed in a bacterial expression system.
authors.affiliation: Vanderbilt University School of Medicine, Nashville, TN, USA.
authors.forename: L C
authors.initials: LC
authors.lastname: Bell-Parikh
authors.forename: N A
authors.initials: NA
authors.lastname: Hosea
authors.forename: M V
authors.initials: MV
authors.lastname: Martin
authors.forename: F P
authors.initials: FP
authors.lastname: Guengerich
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification*
chemical_list: D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System