title: [Protection and mechanism of Fagopyrum cymosum on lung injury in rats with Klebsiella pneumonia].
abstract: OBJECTIVE
  To study the mechanism of protective effect of Fagopyrum cymosum on lung injury induced by Klebsiella pneumonia in rats.
  
  
  METHODS
  The model of rats with Klebsiella pneumonia was established. The male SD rats were randomly divided into control group, model group, Fagopyrum cymosum (6, 3, 1.5 g/kg) three groups, levofloxacin (25 mg/kg) group. The pathological change of lung was observed. The content of IL-1beta, IL-6, IL-8, TNF-alpha, ICAM-1, INF-gamma in serum were measured by radioimmunoassay and Elisa. TNF-alpha, ICAM-1, NF-kappaB p65 protein expressions were measured by immunohistochemistry. MIP-2mRNA expression was detected by in situ hybridization.
  
  
  RESULTS
  The rats of model group had obvious lung injury, but those of Fagopyrum cymosum and levofloxacin groups had less injury. The contents of IL-1beta, IL-6, IL-,8, TNF-alpha, ICAM-1 and INF-gamma in serum and the expressions of TNF-a, ICAM-1, NF-kappaB p65 and MIP--2mRNA of model group were significantly higher than those of the control group (P < 0.05 or P < 0.01), while the indexes of Fagopyrum cymosum and levofloxacin groups were significantly lower than those of model group (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  The lung injury induced by Klebsiella pneumonia is related to TNF-alpha, ICAM-1, NF-kappaB p65 and MIP-2mRNA. To decrease the excessive expression of TNF-alpha, ICAM-1, NF-kappaB p65 and MIP-2mRNA might be the main mechanism of protective effect of Fagopyrum cymosum on lung injury.
authors.affiliation: Department of Pharmacology, Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of Education, Anhui Medical University, Hefei 230032, China. dongly061@yahoo.com.cn
authors.forename: Liu-Yi
authors.initials: LY
authors.lastname: Dong
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Wang
authors.forename: Chang-Qing
authors.initials: CQ
authors.lastname: Wu
authors.forename: Qin
authors.initials: Q
authors.lastname: Jiang
authors.forename: Zhi-Fen
authors.initials: ZF
authors.lastname: Zhang
mesh_terms: D000818:Animals; D054426:Chemokine CXCL2 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000494:pharmacology; D019612:Fagopyrum / Q000737:chemistry; D007150:Immunohistochemistry; D007710:Klebsiella Infections / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects; D064704:Levofloxacin; D008168:Lung / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D008297:Male; D015242:Ofloxacin / Q000008:administration & dosage / Q000494:pharmacology; D018410:Pneumonia, Bacterial / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D054426:Chemokine CXCL2; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D064704:Levofloxacin; D015242:Ofloxacin