title: Peptide-surface adsorption free energy comparing solution conditions ranging from low to medium salt concentrations.
abstract: Multi-technique methods involving surface plasmon resonance spectroscopy and atomic force microscopy provide experimental data for the characterization of peptide adsorption on self-assembled monolayers. A comparative study is carried out in phosphate-buffered saline (PBS) and potassium phosphate-buffered (PPB) water to determine the influence of the salt concentration on the adsorption behavior (see figure; ΔG(0)(ads) : free energy of peptide adsorption, F(des) : force required for peptide desorption).
authors.affiliation: Bioengineering Department, Clemson University, 501 Rhodes Engineering Research Center, Clemson, SC 29634, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wei
authors.forename: Aby A
authors.initials: AA
authors.lastname: Thyparambil
authors.forename: Robert A
authors.initials: RA
authors.lastname: Latour
mesh_terms: D000327:Adsorption; D002021:Buffers; D019277:Entropy; D018625:Microscopy, Atomic Force / Q000379:methods; D010455:Peptides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*; D012492:Salts / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D002021:Buffers; D010455:Peptides; D010710:Phosphates; D017680:Potassium Compounds; D012492:Salts; D012996:Solutions; D014867:Water; C013216:potassium phosphate