title: Heterologous expression and manipulation of three tetracycline biosynthetic pathways.
abstract: A very accommodating host: Three tetracycline biosynthetic pathways were overexpressed and manipulated in the heterologous host Streptomyces lividans K4-114. Through the inactivation of various genes and characterization of the resulting biosynthetic intermediates, new tetracycline-modifying enzymes were identified (see scheme).
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Woncheol
authors.initials: W
authors.lastname: Kim
authors.forename: Lauren B
authors.initials: LB
authors.lastname: Pickens
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
mesh_terms: D002751:Chlortetracycline / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D010118:Oxytetracycline / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism; D013754:Tetracyclines / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D000095702:Polyether Toxins
chemical_list: D002751:Chlortetracycline; D010118:Oxytetracycline; D013754:Tetracyclines; C079467:dactylocycline A; D000095702:Polyether Toxins; C074360:SF 2575