title: Host suitability of house fly, Musca domestica (Diptera: Muscidae), pupae killed by high or low temperature treatment for a parastoid, Spalangia endius (Hymenoptera: Pteromalidae).
abstract: The objective of this study was to establish a high quality progeny production system for the house fly parasitoid, Spalangia endius (Hymenoptera: Pteromalidae), by stockpiling hosts. We performed two host killing methods before host storage: (i) heat-killed by 30 min exposure to 50°C or (ii) freeze-killed by 10 min exposure to -80°C. The average number of parasitoids that emerged from nonstored house fly pupae after heat- or freeze-killing was not significantly different from live pupae. When house fly pupae stored at -20°C after heat-killing were supplied to S. endius, progeny production was significantly less than live pupae. Moreover, productivity became very low when house fly pupae refrigerated at 3°C after heat- or freeze-killing were supplied to S. endius. On the other hand, when house fly pupae stored at -80°C for 1 year after heat-killing were supplied to S. endius, the average number of parasitoids that emerged was not significantly different from live pupae. The average number of parasitoids that emerged from freeze-killed hosts kept for more than 8 weeks at -80°C was significantly fewer than live pupae. Thus, this study clarified that a higher-quality host can be maintained not only by simply storing at -80°C but also by adding heat treatment before storage.
authors.affiliation: Kochi University, Monobe-otsu, Nankoku, Kochi, Japan. kohei.ogw@gmail.com
authors.forename: Kohei
authors.initials: K
authors.lastname: Ogawa
authors.forename: Katsura
authors.initials: K
authors.lastname: Ito
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fukuda
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Tebayashi
authors.forename: Ryo
authors.initials: R
authors.lastname: Arakawa
mesh_terms: D000818:Animals; D061046:Biological Control Agents; D005615:Freezing; D006358:Hot Temperature; D006793:Houseflies / Q000254:growth & development / Q000469:parasitology*; D006927:Hymenoptera / Q000254:growth & development / Q000472:pathogenicity*; D010572:Pest Control, Biological / Q000379:methods; D011679:Pupa / Q000469:parasitology; D013997:Time Factors
chemical_list: D061046:Biological Control Agents