title: Preliminary insights into the phylogeography of six aquatic hyphomycete species.
abstract: Aquatic hyphomycetes occur worldwide on a wide range of plant substrates decomposing in freshwaters, and are known to play a key role in organic matter turnover. The presumed worldwide distribution of many aquatic hyphomycete species has been based on morphology-based taxonomy and identification, which may overlook cryptic species, and mask global-scale biogeographical patterns. This might be circumvented by using DNA sequence data. The internal transcribed spacer (ITS) region from rDNA was recently designated as the most suitable barcode for fungal identification. In this study, we generated ITS barcodes of 130 isolates belonging to 6 aquatic hyphomycete species (Anguillospora filiformis, Flagellospora penicillioides, Geniculospora grandis, Lunulospora curvula, Tetrachaetum elegans and Tricladium chaetocladium), and collected from streams of Southwest Europe (86 isolates) and East Australia (44 isolates). European and Australian populations of 4 species (A. filiformis, F. penicillioides, G. grandis and T. elegans) grouped into different clades, and molecular diversity indices supported significant differentiation. Continents did not share haplotypes, except for T. chaetocladium. Overall results show substantial population diversity for all tested species and suggests that the biogeography of aquatic hyphomycetes may be species-specific.
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar, Braga, Portugal.
authors.forename: Sofia
authors.initials: S
authors.lastname: Duarte
authors.forename: Sahadevan
authors.initials: S
authors.lastname: Seena
authors.forename: Felix
authors.initials: F
authors.lastname: Bärlocher
authors.forename: Fernanda
authors.initials: F
authors.lastname: Cássio
authors.forename: Cláudia
authors.initials: C
authors.lastname: Pascoal
mesh_terms: D001315:Australia; D058893:DNA Barcoding, Taxonomic; D004271:DNA, Fungal / Q000145:classification / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000145:classification / Q000235:genetics*; D017753:Ecosystem; D005060:Europe; D005618:Fresh Water / Q000382:microbiology; D014644:Genetic Variation; D006239:Haplotypes; D003904:Mitosporic Fungi / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D058974:Phylogeography; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer