title: Study of heparin in intestinal ischemia and reperfusion in rats: morphologic and functional evaluation.
abstract: To study whether treatment with heparin (HEP) attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), rats were treated with HEP (100 U/kg intravenously) or saline solution (SS) before I (60 min), which was produced by occlusion of the superior mesenteric artery, and R (120 min). After I or I/R, we mounted 2-cm jejunal segment in an organ bath to study neurogenic contractions stimulated by electrical pulses or KCl, using a digital recording system. Thin jejunal slices were stained with hematoxylin and eosin for optical microscopy. Compared with the sham group, jejunal contractions were similar in the I + HEP and the I/R + HEP groups, but reduced in the I + SS and the I/R + SS groups. The jejunal enteric nerves were damaged in the I + SS and the I/R + SS, but not in the I + HEP and the I/R + HEP cohorts. These results suggested that HEP attenuated intestinal dysfunction caused by I and I/R.
authors.affiliation: Department of Pharmacology, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: M M
authors.initials: MM
authors.lastname: Ghadie
authors.forename: R
authors.initials: R
authors.lastname: Miranda-Ferreira
authors.forename: N S A
authors.initials: NS
authors.lastname: Taha
authors.forename: A S
authors.initials: AS
authors.lastname: Maroso
authors.forename: R J Z
authors.initials: RJ
authors.lastname: Moreti
authors.forename: M P
authors.initials: MP
authors.lastname: Andraus
authors.forename: P
authors.initials: P
authors.lastname: Zempulski
authors.forename: H P
authors.initials: HP
authors.lastname: Monteiro
authors.forename: M J
authors.initials: MJ
authors.lastname: Simões
authors.forename: D J
authors.initials: DJ
authors.lastname: Fagundes
authors.forename: A
authors.initials: A
authors.lastname: Caricati-Neto
authors.forename: M O
authors.initials: MO
authors.lastname: Taha
mesh_terms: D000818:Animals; D019610:Cytoprotection; D004195:Disease Models, Animal; D004558:Electric Stimulation; D017615:Enteric Nervous System / Q000187:drug effects / Q000503:physiopathology; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D005769:Gastrointestinal Motility / Q000187:drug effects; D006493:Heparin / Q000494:pharmacology*; D007583:Jejunum / Q000098:blood supply* / Q000187:drug effects* / Q000294:innervation / Q000473:pathology / Q000503:physiopathology; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*
chemical_list: D005765:Gastrointestinal Agents; D011189:Potassium Chloride; D006493:Heparin