title: Nuclear transferred embryonic stem cells for analysis of B1 B-lymphocyte development.
abstract: The transfer of nuclei of fully differentiated cells into enucleated oocytes is a well-recognized method for the generation of embryonic stem (ES) cells. Here, we demonstrate that nuclear transferred ES (NT-ES) cells can be established with high efficiency using innate-like B lymphocytes as donor cells. We established two mouse lines carrying rearranged immunoglobulin heavy and light chains using NT-ES cells containing nuclei from peritoneal cavity B1 cells. Analysis of B1 clone lines revealed that the B1-cell generation critically depends on the interaction between antigen (possibly self-antigen) and surface immunoglobulin, while the B1-cell maintenance requires the peritoneal environment. The B1-cell expansion takes place in spleen, and is held in check by competitor B2 cells. The results indicate that the NT-ES method could replace the transgenic or knock-in mouse approaches currently used to study the biology of cells that undergo somatic rearrangements of their antigen receptor genes.
authors.affiliation: Laboratory for Autoimmune Regulation, RIKEN, Research Center for Allergy and Immunology, 1-7-22 Suehiro-cho, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Takase
authors.forename: Ryuji
authors.initials: R
authors.lastname: Iida
authors.forename: Mikako
authors.initials: M
authors.lastname: Maruya
authors.forename: Asako
authors.initials: A
authors.lastname: Sakaue-Sawano
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyawaki
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Wakayama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nishigami
authors.forename: Sidonia
authors.initials: S
authors.lastname: Fagarasan
authors.forename: Osami
authors.initials: O
authors.lastname: Kanagawa
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D019070:Cell Lineage / Q000235:genetics; D053595:Embryonic Stem Cells / Q000276:immunology*; D015322:Gene Rearrangement, B-Lymphocyte; D007113:Immunity, Innate / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D053652:Nuclear Transfer Techniques; D010529:Peritoneal Cavity / Q000166:cytology*; D011947:Receptors, Antigen, B-Cell / Q000235:genetics
chemical_list: D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D011947:Receptors, Antigen, B-Cell