title: Label-free DNA hybridization detection by various spectroscopy methods using triphenylmethane dyes as a probe.
abstract: A new assay is developed for direct detection of DNA hybridization using triphenylmethane dye as a probe. It is based on various spectroscopic methods including resonance light scattering (RLS), circular dichroism (CD), ultraviolet spectra and fluorescence spectra, as well as atomic force microscopy (AFM), six triphenylmethane dyes interact with double strand DNA (dsDNA) and single strand DNA (ssDNA) were investigated, respectively. The interaction results in amplified resonance light scattering signals and enables the detection of hybridization without the need for labeling DNA. Mechanism investigations have shown that groove binding occurs between dsDNA and these triphenylmethane dyes, which depends on G-C sequences of dsDNA and the molecular volumes of triphenylmethane dyes. Our present approaches display the advantages of simple and fast, accurate and reliable, and the artificial samples were determined with satisfactory results.
authors.affiliation: Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Tu
authors.forename: Changqun
authors.initials: C
authors.lastname: Cai
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.forename: Lin
authors.initials: L
authors.lastname: Luo
authors.forename: Chao
authors.initials: C
authors.lastname: Weng
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002942:Circular Dichroism; D004396:Coloring Agents / Q000032:analysis*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011110:Polymorphism, Genetic; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D014320:Triphenylmethyl Compounds / Q000032:analysis*; D014783:Viscosity; D015009:Yersinia Infections / Q000382:microbiology; D015008:Yersinia enterocolitica / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004396:Coloring Agents; D004269:DNA, Bacterial; D014320:Triphenylmethyl Compounds; C046945:triphenylmethane; D004247:DNA