title: Identification and isolation of lantibiotics from culture: a bioorthogonal chemistry approach.
abstract: A distinguishing feature of the lantibiotic family of cyclic peptides is the presence of thioethers. Treatment of a lantibiotic with an alkaline solution at high pH gives rise to a β-elimination reaction yielding the corresponding ring opened precursor, containing a dehydro-amino acid residue. We here reveal in a proof-of-concept study that a ring opened lantibiotic (mersacidin) can be captured for pull-down from a culture broth, subsequently released and identified by mass spectrometry.
authors.affiliation: Leiden Institute of Chemistry and Netherlands Proteomics Centre, Gorlaeus Laboratories, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Genevieve
authors.initials: G
authors.lastname: Girard
authors.forename: Bogdan I
authors.initials: BI
authors.lastname: Florea
authors.forename: Paul P
authors.initials: PP
authors.lastname: Geurink
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Gijsbert A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: Mark
authors.initials: M
authors.lastname: Overhand
authors.forename: Herman S
authors.initials: HS
authors.lastname: Overkleeft
authors.forename: Gilles P
authors.initials: GP
authors.lastname: van Wezel
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry / Q000378:metabolism*; D001430:Bacteriocins / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D005285:Fermentation; D015394:Molecular Structure
chemical_list: D001430:Bacteriocins