title: Laser speckle imaging using a consumer-grade color camera.
abstract: Laser speckle imaging (LSI) is a noninvasive optical imaging technique able to provide wide-field two-dimensional maps of moving particles. Raw laser speckle images are typically taken with a scientific-grade monochrome camera. We demonstrate that a digital single-lens reflex (dSLR) camera with a Bayer filter is able to provide similar sensitivity despite taking information only from a specific pixel color. Here we demonstrate the effect of changing three primary dSLR exposure settings (i.e., aperture, exposure time/shutter speed, and gain/sensitivity (ISO)) on speckle contrast. In addition, we present data from an in vivo reactive hyperemia experiment that demonstrates the qualitative similarity in blood-flow dynamics visualized with a color dSLR and a scientific-grade monochrome camera.
authors.affiliation: Department of Biomedical Engineering, University of California, Irvine, 3120 Natural Sciences II, Irvine, California 92697, USA. yango@uci.edu
authors.forename: Owen
authors.initials: O
authors.lastname: Yang
authors.forename: Bernard
authors.initials: B
authors.lastname: Choi
mesh_terms: D003116:Color; D006225:Hand / Q000098:blood supply; D006801:Humans; D006940:Hyperemia / Q000503:physiopathology; D007834:Lasers; D057054:Molecular Imaging; D055096:Optical Devices