title: Aggregation chimeras (ES cell-embryo).
abstract: One approach for generating transgenic mice from ES cell lines is to aggregate ES cells with morula-stage embryos to generate chimeric mice. The chimeras are then bred to generate transgenic offspring. This method offers a simpler and less expensive alternative to the method of ES cell injection into blastocyst embryos.
authors.affiliation: Sunnybrook and Women's College Health, Sciences Centre and the University of Toronto, Toronto, Canada.
authors.forename: Corrinne G
authors.initials: CG
authors.lastname: Lobe
authors.forename: Caiying
authors.initials: C
authors.lastname: Guo
mesh_terms: D000818:Animals; D002678:Chimera; D004624:Embryo Transfer; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D008297:Male; D051379:Mice; D011247:Pregnancy