title: Anti-melanogenesis effect of Glechoma hederacea L. extract on B16 murine melanoma cells.
abstract: Glechoma hederacea L. (Labiatae) has been used in folk medicine to treat various ailments for centuries. We investigated the effects of G. hederacea extract on melanogenesis in B16 melanoma cells. It significantly reduced both the cellular melanin content and tyrosinase activity in a concentration-dependent manner. An MTT assay did not reveal any obvious cytotoxicity. Furthermore, we found that G. hederacea extract decreased tyrosinase and microphthalmia-associated transcription factor protein expression, but did not inhibit tyrosinase-related protein-1 and tyrosinase-related protein-2 expression. RT-PCR analysis indicated that the antimelanogenic effect of G. hederacea extract might be due to inhibition of tyrosinase gene transcription. Moreover, this effect is regulated via suppression of microphthalmia-associated transcription factor protein expression. Our data indicate that G. hederacea extract inhibits melanin synthesis in B16 melanoma cells but is not cytotoxic. Hence it might prove a useful therapeutic agent for treating hyperpigmentation and an effective component of whitening cosmetics.
authors.affiliation: Department of Biochemistry, Akita University Graduate School of Medicine, Akita, Japan.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Qiao
authors.forename: Yukio
authors.initials: Y
authors.lastname: Koizumi
authors.forename: Muxin
authors.initials: M
authors.lastname: Zhang
authors.forename: Miyuki
authors.initials: M
authors.lastname: Natsui
authors.forename: Maria Jolina
authors.initials: MJ
authors.lastname: Flores
authors.forename: Lina
authors.initials: L
authors.lastname: Gao
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Yusa
authors.forename: Souichi
authors.initials: S
authors.lastname: Koyota
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sugiyama
mesh_terms: D000363:Agaricales / Q000201:enzymology; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019686:Lamiaceae / Q000737:chemistry*; D008543:Melanins / Q000096:biosynthesis*; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D010936:Plant Extracts; D014442:Monophenol Monooxygenase