title: A chiral probe for the acute phase proteins alpha-1-acid glycoprotein and alpha-1-antitrypsin based on europium luminescence.
abstract: Reversible and selective binding of a dynamically racemic europium(III) complex to α(1)-acid glycoprotein and α(1)-antitrypsin is characterised by a significant change in the europium total emission spectral fingerprint and the switching on of a large circularly polarised luminescence (CPL) signal from the metal centre. Observation of an induced CD into the ligand chromophore in the presence of α(1)-AGP allows a structure for the protein-bound complex to be postulated. A direct determination of elevated α(1)-AGP levels in human serum was achieved by monitoring changes in the intensity ratio of Eu emission bands.
authors.affiliation: Department of Chemistry, Durham University, Durham, UK.
authors.forename: Rachel
authors.initials: R
authors.lastname: Carr
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Di Bari
authors.forename: Samuele
authors.initials: S
authors.lastname: Lo Piano
authors.forename: David
authors.initials: D
authors.lastname: Parker
authors.forename: Robert D
authors.initials: RD
authors.lastname: Peacock
authors.forename: John M
authors.initials: JM
authors.lastname: Sanderson
mesh_terms: D005063:Europium / Q000737:chemistry*; D006801:Humans; D049449:Luminescence; D009961:Orosomucoid / Q000737:chemistry*; D044967:Serum / Q000737:chemistry; D013237:Stereoisomerism; D000515:alpha 1-Antitrypsin / Q000737:chemistry*
chemical_list: D009961:Orosomucoid; C043392:SERPINA1 protein, human; D000515:alpha 1-Antitrypsin; D005063:Europium