title: Expression of Shewanella oneidensis MR-1 [FeFe]-hydrogenase genes in Anabaena sp. strain PCC 7120.
abstract: H(2) generated from renewable resources holds promise as an environmentally innocuous fuel that releases only energy and water when consumed. In biotechnology, photoautotrophic oxygenic diazotrophs could produce H(2) from water and sunlight using the cells' endogenous nitrogenases. However, nitrogenases have low turnover numbers and require large amounts of ATP. [FeFe]-hydrogenases found in other organisms can have 1,000-fold higher turnover numbers and no specific requirement for ATP but are very O(2) sensitive. Certain filamentous cyanobacteria protect nitrogenase from O(2) by sequestering the enzyme within internally micro-oxic, differentiated cells called heterocysts. We heterologously expressed the [FeFe]-hydrogenase operon from Shewanella oneidensis MR-1 in Anabaena sp. strain PCC 7120 using the heterocyst-specific promoter P(hetN). Active [FeFe]-hydrogenase was detected in and could be purified from aerobically grown Anabaena sp. strain PCC 7120, but only when the organism was grown under nitrate-depleted conditions that elicited heterocyst formation. These results suggest that the heterocysts protected the [FeFe]-hydrogenase against inactivation by O(2).
authors.affiliation: Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, Michigan, USA.
authors.forename: Katrin
authors.initials: K
authors.lastname: Gärtner
authors.forename: Sigal
authors.initials: S
authors.lastname: Lechno-Yossef
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Cornish
authors.forename: C Peter
authors.initials: CP
authors.lastname: Wolk
authors.forename: Eric L
authors.initials: EL
authors.lastname: Hegg
mesh_terms: D000332:Aerobiosis; D017033:Anabaena / Q000235:genetics*; D015870:Gene Expression; D006864:Hydrogenase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D007506:Iron-Sulfur Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D009876:Operon; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000302:isolation & purification; D020592:Shewanella / Q000201:enzymology* / Q000235:genetics
chemical_list: D007506:Iron-Sulfur Proteins; D011994:Recombinant Proteins; C118830:iron hydrogenase; D006864:Hydrogenase