title: Effects of FSH on the expression of receptors for oocyte-secreted factors and members of the EGF-like family during in vitro maturation in cattle.
abstract: FSH induces expansion of bovine cumulus-oocyte complexes (COCs) in cattle, which can be enhanced by oocyte-secreted factors (OSFs). In this study it was hypothesised that FSH stimulates COC expansion in part from direct stimulation of the epidermal growth factor (EGF)-like ligands amphiregulin (AREG), epiregulin (EREG) and betacellulin (BTC), but also in part through regulation of OSFs or their receptors in cumulus cells. Bovine COCs were cultured in defined medium with graded doses of FSH. In the absence of FSH, COCs did not expand. FSH caused cumulus expansion, and increased the abundance of AREG and EREG mRNA in a time- and dose-dependent manner, but decreased BTC mRNA levels. FSH had modest stimulatory effects on the levels of mRNA encoding the bone morphogenetic protein 15 (BMP15) receptor, BMPR1B, in cumulus cells, but did not alter mRNA expression of the growth and differentiation factor 9 (GDF9) receptor, TGFBR1. More interestingly, FSH dramatically stimulated levels of mRNA encoding two receptors for fibroblast growth factors (FGF), FGFR2C and FGFR3C, in cumulus cells. FSH also stimulated mRNA expression of FGFR1B, but not of FGFR2B in cumulus cells. Based on dose-response studies, FGFR3C was the receptor most sensitive to the influence of FSH. This study demonstrates that FSH stimulates the expression of EGF-like factors in bovine cumulus cells, and provides evidence that FSH differently regulates the expression of distinct receptors for OSFs in cumulus cells.
authors.affiliation: Departamento de Farmacologia, Instituto de Biociências, Universidade Estadual Paulista Julio de Mesquita Filho, Rubião Junior, Botucatu, São Paulo 18618-970, Brazil.
authors.forename: Ester Siqueira
authors.initials: ES
authors.lastname: Caixeta
authors.forename: Mariana Fernandes
authors.initials: MF
authors.lastname: Machado
authors.forename: Paula
authors.initials: P
authors.lastname: Ripamonte
authors.forename: Christopher
authors.initials: C
authors.lastname: Price
authors.forename: José
authors.initials: J
authors.lastname: Buratini
mesh_terms: D000003:Abattoirs; D066258:Amphiregulin; D000818:Animals; D066259:Betacellulin; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002417:Cattle / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000662:veterinary; D054885:Cumulus Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D066260:Epiregulin; D005260:Female; D005300:Fertility Agents, Female / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D008024:Ligands; D009865:Oocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D034421:Sus scrofa
chemical_list: D066258:Amphiregulin; D066259:Betacellulin; D066260:Epiregulin; D005300:Fertility Agents, Female; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D008024:Ligands; D020033:Protein Isoforms; D004815:Epidermal Growth Factor; D005640:Follicle Stimulating Hormone; D051497:Receptor, Fibroblast Growth Factor, Type 2; D052005:Bone Morphogenetic Protein Receptors, Type I