title: Lactobacillus plantarum NRIC1832 enhances IL-10 production from CD4⁺ T cells in vitro.
abstract: The anti-inflammatory effect of lactic acid bacteria (LAB) has been reported in several models for autoimmune diseases. It was considered in those studies that IL-10 induced by LAB might have been involved in such anti-inflammatory activity. We therefore examined the IL-10-inducing activity of LAB in detail by using an in vitro culture system of DO11.10 splenocytes. Most strains of LAB tested in this study increased IL-10 production. A further study using one of the tested strains with potent immune-regulatory activity, Lactobacillus plantarum NRIC1832, showed that the enhanced IL-10 was mainly produced by T cells. However, this enhancement required several types of cells other than T cells. NRIC1832 enhanced IL-10 production after short-term exposure to T cells, but this effect diminished after long-term exposure, indicating that the enhancement of IL-10 production by NRIC1832 was temporary, in contrast to the enhancement of IFN-γ production which was still apparent after long-term exposure.
authors.affiliation: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Sayo
authors.initials: S
authors.lastname: Noguchi
authors.forename: Makoto
authors.initials: M
authors.lastname: Hattori
authors.forename: Hisashi
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Akihiro
authors.initials: A
authors.lastname: Hanaoka
authors.forename: Sanae
authors.initials: S
authors.lastname: Okada
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D002454:Cell Differentiation; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis*; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D013154:Spleen / Q000166:cytology
chemical_list: D016753:Interleukin-10; D007371:Interferon-gamma