title: Pigment epithelium derived factor inhibits the growth of human endometrial implants in nude mice and of ovarian endometriotic stromal cells in vitro.
abstract: Angiogenesis is a prerequisite for the formation and development of endometriosis. Pigment epithelium derived factor (PEDF) is a natural inhibitor of angiogenesis. We previously demonstrated a reduction of PEDF in the peritoneal fluid, serum and endometriotic lesions from women with endometriosis compared with women without endometriosis. Here, we aim to investigate the inhibitory effect of PEDF on human endometriotic cells in vivo and in vitro. We found that PEDF markedly inhibited the growth of human endometrial implants in nude mice and of ovarian endometriotic stromal cells in vitro by up-regulating PEDF expression and down-regulating vascular endothelial growth factor (VEGF) expression. Moreover, apoptotic index was significantly increased in endometriotic lesions in vivo and endometriotic stromal cells in vitro when treated with PEDF. In mice treated with PEDF, decreased microvessel density labeled by Von Willebrand factor but not by α-Smooth Muscle Actin was observed in endometriotic lesions. And it showed no increase in PEDF expression of the ovary and uterus tissues. These findings suggest that PEDF gene therapy may be a new treatment for endometriosis.
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Sun
authors.forename: Xuan
authors.initials: X
authors.lastname: Che
authors.forename: Libo
authors.initials: L
authors.lastname: Zhu
authors.forename: Mengdan
authors.initials: M
authors.lastname: Zhao
authors.forename: Guofang
authors.initials: G
authors.lastname: Fu
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Huang
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Hu
authors.forename: Xinmei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004715:Endometriosis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D004717:Endometrium / Q000098:blood supply* / Q000187:drug effects* / Q000473:pathology; D005136:Eye Proteins / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D055806:Microvessels / Q000187:drug effects; D009389:Neovascularization, Pathologic; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015843:Serpins / Q000494:pharmacology*; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014841:von Willebrand Factor / Q000235:genetics / Q000378:metabolism; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D000199:Actins; D005136:Eye Proteins; D009414:Nerve Growth Factors; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D014841:von Willebrand Factor; D000099174:Pigment Epithelium-Derived Factor; C544013:alpha-smooth muscle actin, mouse; C467480:vascular endothelial growth factor A, mouse