title: [Molecular authentication of Fallopia multiflora by PCR-RFLP based on the trnL-trnF analysis].
abstract: OBJECTIVE
  To establish a method for the molecular authentication of Fallopia multiflora.
  
  
  METHODS
  The trnL-trnF regions of Fallopia multiflora and its closely related species and/or adulterants were sequenced and analyzed.
  
  
  RESULTS
  It was found that the trnL-trnF sequence divergences between Fallopia multiflora and its closely related species and/or adulterants were 2.1%-22%. While the intra-species trnL-trnF divergences of Fallopia multiflora were 0%-1.5%. Based on the trnL-trnF regional variations, an endonuclease Xba I (T CTAGA) restriction site specific to Fallopia multiflora was detected. The Fallopia multiflora trnL-F polymerase chain reaction product could be cleaved by Xba I into two pieces, 804-819 bp and 256 bp each, whereas the restriction endonuclease could not digest the trnL-trnF polymerase chain reaction product of its closely related species or adulterants. The restriction patterns analyzed for restriction enzyme Xba I were found to be identical in all Fallopia multiflora individuals from different geographical regions in China.
  
  
  CONCLUSIONS
  The assay based on polymerase chain reaction amplification of the trnL-trnF fragment of chloroplast DNA and subsequent restriction fragment length polymorphism can be used as a general test to identify Fallopia multiflora.
authors.affiliation: School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, China. z_c_jin@163.com
authors.forename: Chuan-Jin
authors.initials: CJ
authors.lastname: Zheng
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Sheng
authors.forename: Shu-Jin
authors.initials: SJ
authors.lastname: Zhao
mesh_terms: D001483:Base Sequence; D018742:DNA, Chloroplast / Q000235:genetics*; D021901:DNA, Intergenic / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D004340:Drug Contamination; D017343:Genes, Plant; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D019664:Polygonaceae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D018742:DNA, Chloroplast; D021901:DNA, Intergenic; D018744:DNA, Plant