title: The I405V and Taq1B polymorphisms of the CETP gene differentially affect sub-clinical carotid atherosclerosis.
abstract: BACKGROUND
  Cholesteryl ester transfer protein (CETP) plays a major role in lipid metabolism, but studies on the association of CETP polymorphisms with risks of cardiovascular disease are inconsistent. This study investigated whether the CETP gene I405V and Taq1B polymorphisms modified subclinical atherosclerosis in an asymptomatic Brazilian population sample.
  
  
  METHODS
  The polymorphisms were analyzed using polymerase chain reaction in 207 adult volunteers. Serum lipid profiles, oxLDL Ab titers, C-reactive protein and tumor necrosis factor-α concentrations and CETP and phospholipid transfer protein (PLTP) activities were determined, and common carotid artery intima-media thickness (cIMT) was measured using ultrasonography.
  
  
  RESULTS
  No differences in cIMT were observed between the presence or absence of the minor B2 and V alleles in either polymorphism. However, inverse correlations between mean cIMT and CETP activity in the presence of these polymorphisms were observed, and positive correlations of these polymorphisms with PLTP activity and oxLDL Ab titers were identified. Moreover, logistic multivariate analysis revealed that the presence of the B2 allele was associated with a 5.1-fold (CI 95%, OR: 1.26 - 21.06) increased risk for cIMT, which was equal and above the 66th percentile and positively interacted with age. However, no associations with the V allele or CETP and PLTP activities were observed.
  
  
  CONCLUSIONS
  None of the studied parameters, including CETP activity, explained the different relationships between these polymorphisms and cIMT, suggesting that other non-determined factors were affected by the genotypes and related to carotid atherosclerotic disease.
authors.affiliation: Lipids Laboratory, Faculty of Medical Sciences, State University of Campinas, Rua 5 de Junho, 350, Campinas, SP 13083-877, Brazil.
authors.forename: Eliane Soler
authors.initials: ES
authors.lastname: Parra
authors.forename: Natália Baratella
authors.initials: NB
authors.lastname: Panzoldo
authors.forename: Denise
authors.initials: D
authors.lastname: Kaplan
authors.forename: Helena Coutinho Franco
authors.initials: HC
authors.lastname: de Oliveira
authors.forename: José Ernesto
authors.initials: JE
authors.lastname: dos Santos
authors.forename: Luiz Sérgio Fernandes
authors.initials: LS
authors.lastname: de Carvalho
authors.forename: Andrei Carvalho
authors.initials: AC
authors.lastname: Sposito
authors.forename: Magnus
authors.initials: M
authors.lastname: Gidlund
authors.forename: Ruy Tsutomu
authors.initials: RT
authors.lastname: Nakamura
authors.forename: Vanessa Helena
authors.initials: VH
authors.lastname: de Souza Zago
authors.forename: Edna Regina
authors.initials: ER
authors.lastname: Nakandakare
authors.forename: Eder Carlos Rocha
authors.initials: EC
authors.lastname: Quintão
authors.forename: Eliana Cotta
authors.initials: EC
authors.lastname: de Faria
mesh_terms: D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood; D001938:Brazil; D002097:C-Reactive Protein / Q000378:metabolism; D002340:Carotid Artery Diseases / Q000097:blood / Q000000981:diagnostic imaging / Q000235:genetics* / Q000276:immunology; D059168:Carotid Intima-Media Thickness; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008055:Lipids / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood / Q000276:immunology; D008297:Male; D008875:Middle Aged; D049294:Phospholipid Transfer Proteins / Q000097:blood; D011110:Polymorphism, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
chemical_list: D001323:Autoantibodies; C506642:CETP protein, human; D053480:Cholesterol Ester Transfer Proteins; D008055:Lipids; D008077:Lipoproteins, LDL; C493430:PLTP protein, human; D049294:Phospholipid Transfer Proteins; D014409:Tumor Necrosis Factor-alpha; C099252:oxidized low density lipoprotein; D002097:C-Reactive Protein