title: p53-Pathway activity and apoptosis in hydrogen sulfide-exposed stem cells separated from human gingival epithelium.
abstract: OBJECTIVE
  Hydrogen sulfide ( H₂S ) is a volatile sulfur compound responsible for physiological halitosis. H₂S was also reported as having periodontal pathologic activities. Gingival crevicular epithelium is the first barrier against periodontal pathogens and their products; oral keratinocyte stem cells OKSCs play key roles in maintaining this barrier. The p53 pathway is responsible for regulating key biological events. Increased apoptosis and cell-cycle arrest of DNA repair can affect keratinocyte stem cells, having a direct impact on the architecture of the oral epithelial tissue. However, the link between H₂S , p53 activity and OKSCs has not yet been fully explored. The main objective of the present study was to explore the implications of the p53 pathway in OKSCs following exposure to H₂S.
  
  
  METHODS
  OKSCs were isolated from human gingival epithelium and incubated with physiological levels of H₂S for 24 and 48 h. Apoptosis and the mitochondrial membrane potential were detected using flow cytometry. Cytochrome c, total p53, phosphorylated p53 and caspase activity were assessed using specific ELISAs. p53 Pathway gene activity was assayed using quantitative RT-PCR.
  
  
  RESULTS
  The levels of apoptosis were significantly increased following incubation in the presence of H₂S, especially after 48 h (36.95 ± 1.91% vs. 4.77 ± 0.74%). Caspases 9 and 3 were activated, whereas caspase-8 activity remained low. Total p53 activity and particularly phosphorylated p53 at serine 46, were significantly enhanced compared with controls (47.11 ± 9.84 units/mL vs. 1.5 ± 0 units/mL and 32.22 ± 10.23 units/mL vs. 0.15 ± 0 units/mL, respectively, at 48 h). Among p53 pathway genes, apoptosis-related genes [i.e. phosphatase and tensin homolog ( PTEN ), B-cell CLL/lymphoma 2 ( BCL2), sirtuin 3 ( SIRT3) and caspases]) were dramatically increased when compared with controls. Moreover, cell-cycle progression genes [i.e. E2F transcription factor (E2F) family and histone deacetylase ( HDAC )] and DNA-repair genes [i.e. growth arrest and DNA-damage-inducible, gamma ( GADD45G ) family and serine/threonine-protein kinase Chk2 ( CHEK2)] were also increased.
  
  
  CONCLUSIONS
  Following incubation with H2 S , OKSCs express multiple p53-associated genes, including programmed cell death, cell-cycle control and DNA-repair genes.
authors.affiliation: Department of Oral Health, Nippon Dental University, Tokyo, Japan.
authors.forename: B
authors.initials: B
authors.lastname: Calenic
authors.forename: K
authors.initials: K
authors.lastname: Yaegaki
authors.forename: N
authors.initials: N
authors.lastname: Ishkitiev
authors.forename: Y
authors.initials: Y
authors.lastname: Kumazawa
authors.forename: T
authors.initials: T
authors.lastname: Imai
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000187:drug effects* / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D059447:Cell Cycle Checkpoints; D004260:DNA Repair / Q000187:drug effects / Q000235:genetics; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006862:Hydrogen Sulfide / Q000633:toxicity*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000187:drug effects*; D016159:Tumor Suppressor Protein p53 / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; D020169:Caspases; D006862:Hydrogen Sulfide