title: Enzymatic activity of human carboxylesterases.
abstract: The carboxylesterases (CEs) are hydrolytic enzymes that metabolize xenobiotics that contain ester, thioester, or amide bonds. CEs are ubiquitously expressed but are found in highest concentration in membrane-enriched fractions of the liver. This unit describes assays used to measure the enzymatic activity and tissue distribution of human CEs. Both spectrophotometric and HPLC-based assays are described in detail. Several of these methods are rapid and easy to perform. These methods will be useful to investigators who are interested in studying the metabolism and disposition of esterified drugs and environmental chemicals.
authors.affiliation: Mississippi State University, Mississippi State, Mississippi, USA.
authors.forename: Matthew K
authors.initials: MK
authors.lastname: Ross
authors.forename: Abdolsamad
authors.initials: A
authors.lastname: Borazjani
mesh_terms: D015153:Blotting, Western; D043182:Carboxylesterase / Q000378:metabolism*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006868:Hydrolysis; D008658:Inactivation, Metabolic; D008099:Liver / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology; D010575:Pesticides / Q000378:metabolism / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013379:Substrate Specificity; D014018:Tissue Distribution; D015262:Xenobiotics / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D010575:Pesticides; D015262:Xenobiotics; D043182:Carboxylesterase