title: Synthesis and anticancer activity of di(3-thienyl)methanol and di(3-thienyl)methane.
abstract: Di(3-thienyl)methanol (2) and di(3-thienyl)methane (3) have been synthesized and screened against the T98G (brain cancer) cell line. Treatment induced cell death (MTT and macro-colony assay), growth inhibition, cytogenetic damage (micronuclei formation), were studied as cellular response parameters. Treatment with the compounds enhanced growth inhibition and cell death in a concentration dependent manner in both T98G and HEK (normal) cell lines. At higher concentrations (>20 µg/mL) the cytotoxic effects of the compounds were highly significant. The effect on clonogenic capacity and micronuclei formation observed after treatment of cells. Amongst the compounds, compound 2 exhibited potent activity against T98G brain cancer cells. Despite potent in vitro activity, both compounds exhibited less cytotoxicity against normal human HEK cells at all effective concentrations.
authors.affiliation: Plasma Bioscience Research Center, Kwangwoon University, Seoul 139-701, Korea.
authors.forename: Nagendra Kumar
authors.initials: NK
authors.lastname: Kaushik
authors.forename: Hong Seon
authors.initials: HS
authors.lastname: Kim
authors.forename: Young June
authors.initials: YJ
authors.lastname: Chae
authors.forename: Young Nam
authors.initials: YN
authors.lastname: Lee
authors.forename: Gi-Chung
authors.initials: GC
authors.lastname: Kwon
authors.forename: Eun Ha
authors.initials: EH
authors.lastname: Choi
authors.forename: In Tae
authors.initials: IT
authors.lastname: Kim
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D057809:HEK293 Cells; D006801:Humans; D007698:Kinesis; D015162:Micronucleus Tests; D013876:Thiophenes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D014410:Tumor Stem Cell Assay
chemical_list: D000970:Antineoplastic Agents; D013876:Thiophenes; C578999:di(3-thienyl)methane; C578998:di(3-thienyl)methanol