title: Use of different levels of ground endophyte-infected tall fescue seed during heat stress to separate characteristics of fescue toxicosis.
abstract: Studies of fescue toxicosis using whole seed diets show reduced feed intake and thermoregulatory ability, but much of the seed passes undigested through the animal. Cattle were fed ground tall fescue seed at different levels to potentially facilitate digestion and absorption of toxins and identify toxin sensitivity for major characteristics of the condition [i.e., hyperthermia, reduced feed intake (FI), reduced blood prolactin]. Steers (n = 18; 350 kg BW) were housed in the Brody Climatology Laboratory at thermoneutrality (TN; 19°C) and randomly assigned to daily diet treatments with either ground endophyte-infected [E+; low and high doses at 20 and 40 μg ergovaline/(kg BW/d), respectively] or endophyte-free [E-; control at 0 μg ergovaline/(kg BW/d)] tall fescue seed. After 12 d at TN, animals received 2 d of transition to heat stress (HS; 36°C daytime, 25°C nighttime) and maintained for 14 more days. Cattle were fed twice daily at 0800 and 1600 h, with water ad libitum. Feed intake was measured at 0700 h, with skin and rectal temperatures, and respiration rate at 0600, 1100, 1600, and 2100 h. Blood was sampled on selected days for prolactin and leptin determinations. Steers fed ground E+ diet decreased (P ≤ 0.0001) FI below controls at TN, with no dose effect. Maximum FI reduction with E+ treatment was 25% at TN, with an additional 46% decrease during HS (P ≤ 0.05). By the end of HS, E+ FI increased (P > 0.05) to that of E-, suggesting recovery. Prolactin was reduced (P ≤ 0.05) in high E+ cattle below controls at study end. Leptin blood concentrations were unaffected by E+ treatment (P > 0.05) but was reduced (P ≤ 0.05) by the end of HS. Pattern of rectal temperature response to HS showed a more rapid initial increase and decline for both E+ groups compared with controls (P ≤ 0.05). Skin temperature was the only variable that identified E+ dose differences. Although there were no treatment differences at TN, skin temperature was lower (P ≤ 0.05) for high E+ steers compared with controls during HS when air temperature was reduced each day. In general, FI was more responsive to E+ toxins than body temperature or blood prolactin, declining even at TN and exhibiting dynamic activity during HS. Although body temperature response to E+ toxins appears to stabilize during HS, this is misleading as rapid change in air temperature exposes effects on skin temperature.
authors.affiliation: Division of Animal Sciences, University of Missouri-Columbia, Columbia 65211, USA. spiersd@missouri.edu
authors.forename: D E
authors.initials: DE
authors.lastname: Spiers
authors.forename: L E
authors.initials: LE
authors.lastname: Wax
authors.forename: P A
authors.initials: PA
authors.lastname: Eichen
authors.forename: G E
authors.initials: GE
authors.lastname: Rottinghaus
authors.forename: T J
authors.initials: TJ
authors.lastname: Evans
authors.forename: D H
authors.initials: DH
authors.lastname: Keisler
authors.forename: M R
authors.initials: MR
authors.lastname: Ellersieck
mesh_terms: D000821:Animal Feed / Q000382:microbiology; D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000139:chemically induced* / Q000382:microbiology / Q000503:physiopathology*; D004032:Diet; D004305:Dose-Response Relationship, Drug; D060026:Endophytes / Q000737:chemistry*; D004879:Ergotamines / Q000008:administration & dosage / Q000494:pharmacology*; D005247:Feeding Behavior / Q000187:drug effects; D031751:Festuca / Q000737:chemistry / Q000382:microbiology*; D006358:Hot Temperature; D020738:Leptin / Q000097:blood; D008297:Male; D011388:Prolactin / Q000097:blood; D011897:Random Allocation; D012639:Seeds / Q000737:chemistry / Q000382:microbiology
chemical_list: D004879:Ergotamines; D020738:Leptin; C076760:ergovaline; D011388:Prolactin