title: Complex I assay in mitochondrial preparations from CNS.
abstract: Mammalian NADH:ubiquinone oxidoreductase (complex I) is made up of at least 46 subunits and is one of the largest enzyme complexes known. It catalyzes the first step of the respiratory electron transport chain through the oxidation of NADH, providing two electrons for the reduction of ubiquinone to ubiquinol, thus propelling protons across the inner membrane of the mitochondria, which subsequently drive ATP synthesis. Dysfunction of complex I has been implicated in various neurodegenerative disorders, and it is probably the most vulnerable component of the electron transport chain to inhibition by reactive oxygen species. We describe a simple spectrophotometric method for estimating the activity of complex I from mitochondria isolated from regions of the central nervous system of mice.
authors.affiliation: National Brain Research Centre, Nainwal Mode, Manesar, India.
authors.forename: Latha
authors.initials: L
authors.lastname: Diwakar
authors.forename: Ajit
authors.initials: A
authors.lastname: Ray
authors.forename: Vijayalakshmi
authors.initials: V
authors.lastname: Ravindranath
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism; D002490:Central Nervous System / Q000201:enzymology* / Q000378:metabolism; D042967:Electron Transport Complex I / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000201:enzymology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology* / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009419:Nerve Tissue Proteins; D042967:Electron Transport Complex I