title: Light sheet microscopy in cell biology.
abstract: Light sheet-based fluorescence microscopy (LSFM) is emerging as a powerful imaging technique for the life sciences. LSFM provides an exceptionally high imaging speed, high signal-to-noise ratio, low level of photo-bleaching, and good optical penetration depth. This unique combination of capabilities makes light sheet-based microscopes highly suitable for live imaging applications. Here, we provide an overview of light sheet-based microscopy assays for in vitro and in vivo imaging of biological samples, including cell extracts, soft gels, and large multicellular organisms. We furthermore describe computational tools for basic image processing and data inspection.
authors.affiliation: Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
authors.forename: Raju
authors.initials: R
authors.lastname: Tomer
authors.forename: Khaled
authors.initials: K
authors.lastname: Khairy
authors.forename: Philipp J
authors.initials: PJ
authors.lastname: Keller
mesh_terms: D000818:Animals; D002457:Cell Extracts / Q000302:isolation & purification; D058948:Cell Tracking / Q000379:methods; D004625:Embryo, Nonmammalian / Q000166:cytology; D005456:Fluorescent Dyes / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D008870:Microtubules / Q000648:ultrastructure*; D012984:Software; D013194:Staining and Labeling; D016610:Tissue Embedding / Q000379:methods
chemical_list: D002457:Cell Extracts; D005456:Fluorescent Dyes