title: Efficient ethanol production from separated parenchyma and vascular bundle of oil palm trunk.
abstract: For efficient utilization of both starchy and cellulosic materials, oil palm trunk was separated into parenchyma (PA) and vascular bundle (VB). High solid-state simultaneous saccharification and fermentation (HSS-SSF) using 30% (w/v) PA, containing 46.7% (w/w) starch, supplemented with amylases and Saccharomyces cerevisiae K3, produced 6.1% (w/v) ethanol. Subsequent alkali-pretreatment using sodium hydroxide was carried out with starch-free PA (sfPA) and VB. Enzymatic digestibility of 5% (w/v) pretreated sfPA and VB was 92% and 97%, respectively, using 18 FPU of commercial cellulase supplemented with 10 U of Novozyme-188 per gram of substrate. Likewise, HSS-SSF using 30% (w/v) alkali-pretreated sfPA and VB, with cellulases and yeast, resulted in high ethanol production (8.2% and 8.5% (w/v), respectively). These results show that HSS-SSF using separated PA and VB is a useful fermentation strategy, without loss of starchy and cellulosic materials, for oil palm trunk.
authors.affiliation: Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 303-8686, Japan.
authors.forename: Panida
authors.initials: P
authors.lastname: Prawitwong
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kosugi
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Arai
authors.forename: Lan
authors.initials: L
authors.lastname: Deng
authors.forename: Kok Chang
authors.initials: KC
authors.lastname: Lee
authors.forename: Darah
authors.initials: D
authors.lastname: Ibrahim
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Murata
authors.forename: Othman
authors.initials: O
authors.lastname: Sulaiman
authors.forename: Rokiah
authors.initials: R
authors.lastname: Hashim
authors.forename: Kumar
authors.initials: K
authors.lastname: Sudesh
authors.forename: Wan Asma Bt
authors.initials: WA
authors.lastname: Ibrahim
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Saito
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Mori
mesh_terms: D003247:Conservation of Natural Resources / Q000379:methods*; D000431:Ethanol / Q000302:isolation & purification* / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000382:microbiology*; D010936:Plant Extracts / Q000378:metabolism*; D012278:Ricinus / Q000382:microbiology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D000431:Ethanol