title: [Effects of rhBMP-7 on osteoblast differentiation of murine bone marrow mesenchymal stem cells in vitro].
abstract: OBJECTIVE
  To study the effects of recombinant human bone morphogenetic protein-7 (rhBMP-7) on osteoblast differentiation of murine bone marrow mesenchymal stem cells (BMSCs).
  
  
  METHODS
  The BMSCs were collected from murine bone marrow by density gradient centrifugation. Cells were adherent cultured and expanded in vitro. RhBMP-7 was added into the culture medium of the 3rd passage BMSCs. Five days later,  we performed alkaline phosphatase (ALP) staining and detected ALP activity and osteocalcin (OC) content. Osteoblast differentiation marker gene including OC and collage I (Col-I) were assayed by RT-PCR. Total protein was isolated and the secretion of Col-I in the cells was measured by Western blotting.
  
  
  RESULTS
  The staining intensity  of ALP in the group with rhBMP-7 was stronger than that in the negative control group. ALP activity and OC content of rhBMP-7 group were obviously higher than that of the negative control group. The mRNA of OC and Col-I and the protein of Col-I were highly expressed in the group induced by rhBMP-7.
  
  
  CONCLUSIONS
  RhBMP-7 can induce murine BMSCs to differentiate into osteoblast in vitro.
authors.affiliation: Department of Pharmacy, Wannan Medical University, Wuhu, China.
authors.forename: Guo-dong
authors.initials: GD
authors.lastname: Wang
authors.forename: Shao-yun
authors.initials: SY
authors.lastname: Wu
authors.forename: Jin-xiang
authors.initials: JX
authors.lastname: Han
authors.forename: Shi-li
authors.initials: SL
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism
chemical_list: D055419:Bone Morphogenetic Protein 7; D024042:Collagen Type I; D015675:Osteocalcin; D000469:Alkaline Phosphatase