title: Characterization and comparison of methacrylic acid with 2-acrylamido-2-methyl-1-propanesulfonic acid in the preparation of monolithic column for capillary electrochromatography.
abstract: Butyl methacrylate (BMA)-ethylene dimethacrylate (EDMA)-methacrylic acid (MAA) and BMA-EDMA-2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS) monolithic columns were prepared by varying the percentage of ionic monomers for capillary electrochromatography. Monolithic columns with a higher content of ionic monomers provided better column efficiency, and the performance of BMA-EDMA-MAA monoliths was better than BMA-EDMA-AMPS. To characterize and optimize BMA-EDMA-MAA monoliths, the effects of the content of cross-linker and the total monomer in the polymerization mixture on column performance were also studied. Plate heights of 8.2 µm for the unretained solute (thiourea) and 12.6 µm for the retained solute (naphthalene) were achieved with a monolithic column using 2.5% MAA (Column I).
authors.affiliation: Department of Environmental and Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Horiguchi
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.forename: Tomoko
authors.initials: T
authors.lastname: Masunaga
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Fujita
authors.forename: Marwa Fathy Bakr
authors.initials: MF
authors.lastname: Ali
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000476:Alkanesulfonates / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000295:instrumentation*; D008689:Methacrylates / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D000476:Alkanesulfonates; D008689:Methacrylates; D011108:Polymers; C008384:methacrylic acid; C056288:2-acrylamido-2-methylpropanesulfonate