title: [Study on water-soluble constituents of Fritillaria unibracteata by HPLC fingerprints].
abstract: OBJECTIVE
  To establish a HPLC fingerprint of water-soluble constituents of Fritillaria unibracteata.
  
  
  METHODS
  Zorbax SB Aq C18 chromatographic column (4. 6 mm x 250 mm, 5 microm) was adopted for gradient elute with the mobile phase consisting of methanol and water. The flow rate was 1.0 mL x min(-1); the detection wavelength was 260 nm, and the temperature of sample manager was set at 25 degrees C. Similarity Evaluation System for Chromatographic Fingerprint of traditional Chinese medicine (version 2.0) published by the State Pharmacopeia Committee of China was adopted for the fingerprint analysis on the 11 batches of F. unibracteata herbs.
  
  
  RESULTS
  The 11 batches of F. unibracteata herbs had 14 common peaks, nine of which were identified with good separating degrees. The similarities of the 11 batches were more than 0. 970, with good quality homogeneity.
  
  
  CONCLUSIONS
  The method is so accurate, highly reproducible and stable that it is suitable for the comprehensive quality evaluation of F. unibracteata herbs.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Duan
authors.forename: Shilin
authors.initials: S
authors.lastname: Chen
authors.forename: Dahua
authors.initials: D
authors.lastname: Wei
authors.forename: Linfang
authors.initials: L
authors.lastname: Huang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003878:Dermatoglyphics; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D031410:Fritillaria / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D014867:Water