title: Purification and characterisation of antibacterial peptide-containing compound derived from palm kernel cake.
abstract: Palm kernel cake (PKC), the most useful by-product resulted from palm kernel oil production. In this study, PKC-derived protein product was found suitable for use as an antimicrobial agent with potent antibacterial activity, particularly against Bacillus species, after enzymatic hydrolysis with alcalase. The hydrolysate was further purified by gel filtration chromatography. The purified fraction was found to have 14.63±0.70% (w/w) protein, a molecular mass of 2.4kDa and low hemolytic activity (<50% hemolysis of human erythrocytes at concentration of 1000μg/ml). The presence of lysine and the major component lauric acid derivative, as indicated by electrospray ionisation-mass spectrometry (ESI-MS) direct infusion and nuclear magnetic resonance (NMR) spectroscopy, may have contributed to the antibacterial effect of purified PKC fraction. This study suggests that the antibacterial PKC compound may be not a pure peptide but instead a peptide-containing compound high in lauric acid derivative.
authors.affiliation: Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
authors.forename: Yen Nee
authors.initials: YN
authors.lastname: Tan
authors.forename: Mohd Khan
authors.initials: MK
authors.lastname: Ayob
authors.forename: Wan Ahmad
authors.initials: WA
authors.lastname: Wan Yaacob
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D001407:Bacillus / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects; D006801:Humans; D007220:Industrial Waste / Q000032:analysis*; D008970:Molecular Weight; D000073878:Palm Oil; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry*; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D007220:Industrial Waste; D010455:Peptides; D010938:Plant Oils; D011492:Protein Hydrolysates; D000073878:Palm Oil