title: Transcription of the pain-related TRPV1 gene requires Runx1 and C/EBPβ factors.
abstract: Transient Receptor Potential Vanilloid type 1 channel (TRPV1) is an important endogenous transducer of noxious heat and chemical stimuli and is required during development of inflammatory hypersensitivity. The transcription factor Runx1 is known to play a relevant role in sensory neuron differentiation as it controls the expression of several sensory nociceptive receptors, including TRPV1. Here, we show that Runx1 up-regulates TRPV1 transcription activity by interacting directly with the proximal TRPV1 gene promoter sequence. Importantly, C/EBPβ a well-established heterodimer partner of Runx1 also binds to the TRPV1 promoter and cooperates with Runx1 to further stimulate TRPV1 transcription. Our results support a mechanism where Runx1-C/EBPβ-containing transcription regulatory complexes are recruited to the TRPV1 gene promoter to modulate TRPV1 expression in dorsal root ganglia neurons.
authors.affiliation: Center for Biomedical Research, Universidad Andres Bello, Santiago, Chile.
authors.forename: Giorgia D
authors.initials: GD
authors.lastname: Ugarte
authors.forename: Emilio
authors.initials: E
authors.lastname: Diaz
authors.forename: Miguel
authors.initials: M
authors.lastname: Biscaia
authors.forename: Jimmy
authors.initials: J
authors.lastname: Stehberg
authors.forename: Martin
authors.initials: M
authors.lastname: Montecino
authors.forename: Brigitte
authors.initials: B
authors.lastname: van Zundert
mesh_terms: D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics* / Q000378:metabolism; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics* / Q000378:metabolism; D005727:Ganglia, Spinal / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D016716:PC12 Cells; D010146:Pain / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D051381:Rats; D050916:TRPV Cation Channels / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D015854:Up-Regulation
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D050676:Core Binding Factor Alpha 2 Subunit; C493727:Runx1 protein, rat; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat