title: Studying the effects of genistein on gene expression of fish embryos as an alternative testing approach for endocrine disruption.
abstract: Assessment of endocrine disruption currently relies on testing strategies involving adult vertebrates. In order to minimize the use of animal tests according to the 3Rs principle of replacement, reduction and refinement, we propose a transcriptomics and fish embryo based approach as an alternative to identify and analyze an estrogenic activity of environmental chemicals. For this purpose, the suitability of 48 h and 7 days post-fertilization zebrafish and medaka embryos to test for estrogenic disruption was evaluated. The embryos were exposed to the phytoestrogen genistein and subsequently analyzed by microarrays and quantitative real-time PCR. The functional analysis showed that the genes affected related to multiple metabolic and signaling pathways in the early fish embryo, which reflect the known components of genistein's mode of actions, like apoptosis, estrogenic response, hox gene expression and steroid hormone synthesis. Moreover, the transcriptomic data also suggested a thyroidal mode of action and disruption of the nervous system development. The parallel testing of two fish species provided complementary data on the effects of genistein at gene expression level and facilitated the separation of common from species-dependent effects. Overall, the study demonstrated that combining fish embryo testing with transcriptomics can deliver abundant information about the mechanistic effects of endocrine disrupting chemicals, rendering this strategy a promising alternative approach to test for endocrine disruption in a whole organism in-vitro scale system.
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology, Aachen, Germany. viktoria.schiller@ime.fraunhofer.de
authors.forename: Viktoria
authors.initials: V
authors.lastname: Schiller
authors.forename: Arne
authors.initials: A
authors.lastname: Wichmann
authors.forename: Ralf
authors.initials: R
authors.lastname: Kriehuber
authors.forename: Elke
authors.initials: E
authors.lastname: Muth-Köhne
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.forename: Markus
authors.initials: M
authors.lastname: Hecker
authors.forename: Martina
authors.initials: M
authors.lastname: Fenske
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000196:embryology / Q000378:metabolism*; D052244:Endocrine Disruptors / Q000494:pharmacology*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D019833:Genistein / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D009990:Oryzias / Q000196:embryology / Q000235:genetics*; D048789:Phytoestrogens / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
chemical_list: D052244:Endocrine Disruptors; D048789:Phytoestrogens; D019833:Genistein