title: A multi-omic map of the lipid-producing yeast Rhodosporidium toruloides.
abstract: Triacylglycerols are among the most attractive alternative raw materials for biofuel development. Current oil plant-based technologies are limited in terms of triacylglycerol production capacity and rate. These limitations may be circumvented by biotransformation of carbohydrates into lipids; however, our understanding of microbial oleaginicity remains limited. Here we present the results of a multi-omic analysis of Rhodosporidium toruloides, a robust triacylglycerol-producing fungus. The assembly of genome and transcriptome sequencing data reveals a genome of 20.2 Mb containing 8,171 protein-coding genes, the majority of which have multiple introns. Genes including a novel fatty acid synthase are predicted to participate in metabolic pathways absent in non-oleaginous yeasts. Transcriptomic and proteomic data suggest that lipid accumulation under nitrogen-limited conditions correlates with the induction of lipogenesis, nitrogenous compound recycling, macromolecule metabolism and autophagy. The multi-omic map of R. toruloides therefore provides a valuable resource for efforts to rationally engineer lipid-production pathways.
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian 116023, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhu
authors.forename: Sufang
authors.initials: S
authors.lastname: Zhang
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Shen
authors.forename: Xinping
authors.initials: X
authors.lastname: Lin
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Yongjin J
authors.initials: YJ
authors.lastname: Zhou
authors.forename: Guojie
authors.initials: G
authors.lastname: Jin
authors.forename: Mingliang
authors.initials: M
authors.lastname: Ye
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
authors.forename: Hanfan
authors.initials: H
authors.lastname: Zou
authors.forename: Zongbao K
authors.initials: ZK
authors.lastname: Zhao
mesh_terms: D001487:Basidiomycota / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D023281:Genomics / Q000379:methods*; D008055:Lipids / Q000096:biosynthesis*; D040901:Proteomics / Q000379:methods*
chemical_list: D008055:Lipids