title: Alterations in antioxidant enzyme activities and proline content in pea leaves under long-term drought stress.
abstract: The effects of long-term drought stress on chlorophyll, proline, protein and hydrogen peroxide (H2O2) contents, malondialdehyde (MDA) in terms of lipid peroxidation and on the changes in the activities of antioxidant enzymes such as superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), catalase (CAT, EC 1.11.1.6) and peroxidase (POX; EC 1.11.1.7) in the leaves of pea (Pisum sativum L.) were studied in field conditions. Chlorophyll and protein contents in leaves decreased significantly with increased drought stress. The proline content increased markedly under water deficit. MDA amounts were elevated as a result of water shortage, whereas H(2)O(2) content changed slightly in pea leaves exposed to drought stress. Drought stress markedly enhanced the activities of SOD, CAT and POX but slightly changed the activity of APX. We conclude that in field conditions, long-term water shortage increased the susceptibility to drought in peas.
authors.affiliation: Faculty of Science and Arts, Department of Biology, Gaziosmanpaşa University, Tokat, Turkey.
authors.forename: Ilhami
authors.initials: I
authors.lastname: Karataş
authors.affiliation: Faculty of Science and Arts, Department of Biology, Gaziosmanpaşa University, Tokat, Turkey.
authors.forename: Lokman
authors.initials: L
authors.lastname: Öztürk
authors.affiliation: Faculty of Education, Department of Biology, Ataturk University, Erzurum, Turkey ydemir_409@yahoo.com.
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Demir
authors.affiliation: Faculty of Agriculture, Department of Agricultural Structures and Irrigation, Erciyes University, Kayseri, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Unlükara
authors.affiliation: Faculty of Agriculture, Department of Agricultural Structures and Irrigation, Akdeniz University, Antalya, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Kurunç
authors.affiliation: Faculty of Science, Department of Biology, Karatekin University, Çankırı, Turkey.
authors.forename: Oral
authors.initials: O
authors.lastname: Düzdemir
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D055864:Droughts; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008315:Malondialdehyde / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D018532:Pisum sativum / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D011392:Proline / Q000032:analysis / Q000378:metabolism*; D012987:Soil / Q000737:chemistry; D013312:Stress, Physiological / Q000502:physiology*; D014867:Water / Q000378:metabolism
chemical_list: D000975:Antioxidants; D012987:Soil; D014867:Water; D008315:Malondialdehyde; D011392:Proline; D006861:Hydrogen Peroxide; D010088:Oxidoreductases