title: Mimotope peptides selected from phage display combinatorial library by serum antibodies of pigs experimentally infected with Taenia solium as leads to developing diagnostic antigens for human neurocysticercosis.
abstract: Neurocysticercosis is caused by penetration of the tapeworm Taenia solium larvae into the central nervous system resulting in a diverse range of neurologic complications including epilepsy in endemic areas that globalization spreads worldwide. Sensitive and specific immunodiagnosis is needed for the early detection and elimination of the parasite, but the lack of standardized, readily obtainable antigens is a challenge. Here, we used the phage display for resolving the problem. The rationale of the strategy rests on the concept that the screening of combinatorial libraries with polyclonal serum to pathogens reveals families of peptides mimicking the pathogen most immunodominant epitopes indispensable for the successful diagnosis. The screening of a 7mer library with serum IgG of four pigs experimentally infected with parasite followed by computer aided segregation of the selected sequences resulted in the discovery of four clusters of homologous sequences of which one presented a family of ten mimotopes selected by three infected pig serum IgGs; the common motif sequence LSPF carried by the family was considered to be the core of an immunodominant epitope of the parasite critical for the binding with the antibody that selected the mimotopes. The immunoassay testing permitted to select a mimotope whose synthetic peptide free of the phage with the amino acid sequence Leu-Ser-Fen-Pro-Ser-Val-Val that distinguished well a panel of 21 cerebrospinal fluids of neurocysticercosis patients from the fluids of individuals with neurological complications of other etiology. This peptide is proposed as a lead for developing a novel molecularly defined diagnostic antigen(s) for the neurocysticercosis.
authors.affiliation: Department of Medicinal Genomics and Environmental Toxicology, Institute of Biomedical Research of the Mexican National Autonomous University, Mexico City, University Campus, CP 04510, Mexico. karlen@unam.mx
authors.forename: Karlen
authors.initials: K
authors.lastname: Gazarian
authors.forename: Merril
authors.initials: M
authors.lastname: Rowlay
authors.forename: Tatiana
authors.initials: T
authors.lastname: Gazarian
authors.forename: Jorge Enrique
authors.initials: JE
authors.lastname: Vazquez Buchelli
authors.forename: Marisela
authors.initials: M
authors.lastname: Hernández Gonzáles
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D000937:Antigen-Antibody Reactions; D000947:Antigens, Helminth / Q000097:blood / Q000276:immunology*; D001435:Bacteriophages / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D020019:Neurocysticercosis / Q000175:diagnosis* / Q000276:immunology; D009842:Oligopeptides / Q000097:blood / Q000737:chemistry* / Q000276:immunology; D019151:Peptide Library; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000469:parasitology; D041201:Taenia solium / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G; D009842:Oligopeptides; D019151:Peptide Library