title: Structural features of N-glycans of seaweed glycoproteins: predominant occurrence of high-mannose type N-glycans in marine plants.
abstract: We analyzed structural features of N-glycans linked to glycoproteins expressed in various seaweeds to identify new sources of biologically-important N-glycans or N-glycopeptides. Structural analysis of the N-glycans of glycopeptides prepared from pepsin digests of 15 species of seaweed revealed that only high-mannose type N-glycans occur in seaweed glycoproteins, and the Man₉GlcNAc₂ structure predominates in Sargassum fulvellum and Zostera marina, while no typical plant complex type N-glycans bearing β1-2 xylosyl and α1-3 fucosyl residues present in either algae or seagrass. These results indicate that seaweeds lack the activities of several of the glycosyltransferases required for the biosynthesis of the complex type N-glycans found in terrestrial plants, and that the context of N-glycan processing in seaweeds is different from that in terrestrial plant cells.
authors.affiliation: Department of Biofunctional Chemistry, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Yoshiie
authors.forename: Megumi
authors.initials: M
authors.lastname: Maeda
authors.forename: Mariko
authors.initials: M
authors.lastname: Kimura
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Hama
authors.forename: Motoharu
authors.initials: M
authors.lastname: Uchida
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D018506:Gene Expression Regulation, Plant; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D008358:Mannose; D011134:Polysaccharides / Q000737:chemistry*; D012624:Seaweed / Q000737:chemistry*
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins; D011134:Polysaccharides; D008358:Mannose