title: Revisiting the housekeeping genes of human placental development and insufficiency syndromes.
abstract: Gene expression analysis using semi-quantitative RT-PCR is a common tool in placental research. However the comparison of steady-state gene transcription between different clinical groups is dependent upon comparison of target mRNA data with mRNA obtained from so-called housekeeping (HK) genes whose steady-state transcription does not differ significantly between the groups. In this communication, we evaluated the performance of candidate HK genes across nine clinical groups commonly used in placental research. We used the GeNorm method to evaluate qRT-PCR data to determine the performance of candidate HKs.
authors.affiliation: Centre for Research in Women's and Infant's health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 25 Orde Street #6-1020, Toronto, ON, Canada M5T 3H7. Drewlo@lunenfeld.ca
authors.forename: S
authors.initials: S
authors.lastname: Drewlo
authors.forename: K
authors.initials: K
authors.lastname: Levytska
authors.forename: J
authors.initials: J
authors.lastname: Kingdom
mesh_terms: D000328:Adult; D005260:Female; D005317:Fetal Growth Retardation / Q000378:metabolism / Q000503:physiopathology; D018507:Gene Expression Regulation, Developmental; D020043:Genes, Essential; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D010929:Placentation; D011225:Pre-Eclampsia / Q000378:metabolism / Q000503:physiopathology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism*; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012720:Severity of Illness Index; D047929:Term Birth; D014015:Tissue Banks; D055815:Young Adult
chemical_list: D011257:Pregnancy Proteins; D012333:RNA, Messenger