title: Cell death in HeLa cells upon imperatorin and cisplatin treatment.
abstract: There is growing evidence that commonly applied chemotherapy regimens can be improved by introducing new, specific, active and low side-effect drugs, or by combining substances to obtain the required clinical effect. The aim of the present study was to investigate the effects of imperatorin and cisplatin, applied separately or in combination, on apoptosis, necrosis and autophagy induction in the human cervical carcinoma cell line(HeLa). Imperatorin appeared to be a potent autophagy inducer, rather than a necrotic or apoptotic one. In contrast, cisplatin induced mainly apoptosis and necrosis after 6 h and 24 h, while longer incubation resulted only in necrosis induction. When HeLa cells were incubated with both drugs, autophagy appeared most frequently,although to a smaller extent than that observed after imperatorin administered alone. At the molecular level, autophagy was correlated with the presence of the cleaved form of microtubule-associated protein 1 light chain LC3 - LC3II. It was also accompanied by the inhibition of heat shock proteins Hsp27 and Hsp72 expression.Our results indicate that imperatorin alone, or in combination with cisplatin, is mainly an autopahgy inducer in HeLa cells.
authors.affiliation: Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Lublin, Poland. jjgil@poczta.umcs.lublin.pl
authors.forename: Joanna
authors.initials: J
authors.lastname: Jakubowicz-Gil
authors.forename: Roman
authors.initials: R
authors.lastname: Paduch
authors.forename: Zofia
authors.initials: Z
authors.lastname: Ulz
authors.forename: Dorota
authors.initials: D
authors.lastname: Badziul
authors.forename: Kazimierz
authors.initials: K
authors.lastname: Głowniak
authors.forename: Antoni
authors.initials: A
authors.lastname: Gawron
mesh_terms: D001343:Autophagy / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D011564:Furocoumarins / Q000494:pharmacology*; D055551:HSP27 Heat-Shock Proteins / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009336:Necrosis
chemical_list: D011564:Furocoumarins; D055551:HSP27 Heat-Shock Proteins; D050884:HSP72 Heat-Shock Proteins; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D053148:Caspase 3; C031534:imperatorin; D002945:Cisplatin