title: OEM--a new medium for rapid isolation of onion-pathogenic and onion-associated bacteria.
abstract: Onions (Allium cepa L.) are plagued by a number of bacterial pathogens including Pantoea ananatis, P. agglomerans, Burkholderia cepacia, Enterobacter cloacae, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas axonopodis pv. axonopodis and several Pseudomonas spp. We developed a semi-selective medium, termed onion extract medium (OEM), to selectively and rapidly isolate bacteria pathogenic to and associated with onions and onion-related samples including bulbs, seeds, sets, transplant seedlings, soil and water. Most strains of interest grow sufficiently on OEM in 24h at 28°C for tentative identification based on colony morphology, facilitating further characterization by microbiological and/or molecular means.
authors.affiliation: Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY 14853, USA. amz44@cornell.edu
authors.forename: Ali M
authors.initials: AM
authors.lastname: Zaid
authors.forename: Jean M
authors.initials: JM
authors.lastname: Bonasera
authors.forename: Steven V
authors.initials: SV
authors.lastname: Beer
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods*; D019697:Onions / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*
chemical_list: D003470:Culture Media