title: A photochromic bacterial photoreceptor with potential for super-resolution microscopy.
abstract: We introduce a novel fluorescent reporter with potential for super-resolution microscopy, based on the bacterial photoreceptor YtvA. YtvA (from Bacillus subtilis) comprises a photosensitive flavin-based LOV domain, efficiently photo-switchable between fluorescent and non-fluorescent states. We demonstrate Fluorescence PhotoActivation Localization Microscopy (FPALM) studies of live Escherichia coli cells, expressing YtvA molecules.
authors.affiliation: Dipartimento di Fisica, Università di Parma, viale delle Scienze 7A, 43124 Parma, Italy.
authors.forename: Aba
authors.initials: A
authors.lastname: Losi
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Gärtner
authors.forename: Sarah
authors.initials: S
authors.lastname: Raffelberg
authors.forename: Francesca
authors.initials: F
authors.lastname: Cella Zanacchi
authors.forename: Paolo
authors.initials: P
authors.lastname: Bianchini
authors.forename: Alberto
authors.initials: A
authors.lastname: Diaspro
authors.forename: Carmen
authors.initials: C
authors.lastname: Mandalari
authors.forename: Stefania
authors.initials: S
authors.lastname: Abbruzzetti
authors.forename: Cristiano
authors.initials: C
authors.lastname: Viappiani
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D008164:Luminescent Proteins / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D020283:Photoreceptors, Microbial / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D020283:Photoreceptors, Microbial