title: Digital confocal microscope.
abstract: We demonstrate experimentally a scanning confocal microscopy technique based on digital holography. The method relies on digital holographic recording of the scanned spot. The data collected in this way contains all the necessary information to digitally produce three-dimensional images. Several methods to treat the data are presented. Examples of reflection and transmission images of epithelial cells and mouse brain tissue are shown.
authors.affiliation: Optics Laboratory, École Polytechnique Fédérale de Lausanne, Station 17, Lausanne 1015, Switzerland. alexandre.goy@epfl.ch
authors.forename: Alexandre S
authors.initials: AS
authors.lastname: Goy
authors.forename: Demetri
authors.initials: D
authors.lastname: Psaltis
mesh_terms: D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation*; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D018613:Microscopy, Confocal / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*