title: A protein from Pleurotus eryngii var. tuoliensis C.J. Mou with strong removal activity against the natural steroid hormone, estriol: purification, characterization, and identification as a laccase.
abstract: A protein with strong removal activity against the natural estrogen estriol was purified from a culture supernatant of Pleurotus eryngii var. tuoliensis C.J. Mou. The protein was characterized as a laccase and had a molecular mass of 60kDa on SDS-PAGE. The enzyme was most active at pH 7.0 and 50°C. The partial N-terminal amino acid sequence of the enzyme showed homology with laccases from mushrooms, such as Pleurotus ostreatus, Coriolus versicolor (current name: Trametes versicolor), Pycnoporus cinnabarinus, and P. eryngii. A recombinant yeast assay confirmed that laccase treatment was very efficient for removing the estrogenic activity of steroid estrogens. Our results suggest that the enzyme may be applicable as a potential factor for removing natural steroid hormones.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. mueda@biochem.osakafu-u.ac.jp
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Ueda
authors.forename: Kayo
authors.initials: K
authors.lastname: Shintani
authors.forename: Akiko
authors.initials: A
authors.lastname: Nakanishi-Anjyuin
authors.forename: Masami
authors.initials: M
authors.lastname: Nakazawa
authors.forename: Mizuho
authors.initials: M
authors.lastname: Kusuda
authors.forename: Fumiki
authors.initials: F
authors.lastname: Nakatani
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawaguchi
authors.forename: Sho-Ichi
authors.initials: S
authors.lastname: Tsujiyama
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kawanishi
authors.forename: Takashi
authors.initials: T
authors.lastname: Yagi
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Miyatake
mesh_terms: D000595:Amino Acid Sequence; D001709:Biotechnology; D004795:Enzyme Stability; D004964:Estriol / Q000302:isolation & purification* / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D020076:Pleurotus / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D014874:Water Pollutants, Chemical; D042845:Laccase; D004964:Estriol