title: Recombinant portal protein from Staphylococcus epidermidis bacteriophage CNPH82 is a 13-subunit oligomer.
abstract: The portal protein cn3 of bacteriophage CNPH82 is predicted to serve as a gateway for translocation of viral genome into preformed pro-capsid, like portal proteins from other double-stranded DNA tailed bacteriophages. The host of bacteriophage CNPH82 is the opportunistic human pathogenic bacterium Staphylococcus epidermidis, a major cause of nosocomial infections. The portal protein of this phage has been cloned, overexpressed and purified. Size-exclusion chromatography-multi-angle laser light scattering analysis has indicated that the portal protein contains ∼13 subunits. Crystals of the portal protein, diffracting to 4.2 Å, have been obtained. These crystals belong to the space group C222(1) with the unit-cell parameters of a = 252.4, b = 367.0, c = 175.5 Å. The self-rotation function revealed the presence of a single 13-subunit oligomer in the asymmetric unit.
authors.affiliation: York Structural Biology Laboratory, Department of Chemistry, University of York, York, YO10 5DD, England.
authors.forename: Weisha
authors.initials: W
authors.lastname: Luan
authors.forename: Jochen
authors.initials: J
authors.lastname: Fesseler
authors.forename: Maria
authors.initials: M
authors.lastname: Chechik
authors.forename: Carina R
authors.initials: CR
authors.lastname: Buttner
authors.forename: Alfred A
authors.initials: AA
authors.lastname: Antson
authors.forename: Callum
authors.initials: C
authors.lastname: Smits
mesh_terms: D021122:Protein Subunits / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D013204:Staphylococcus Phages / Q000737:chemistry*; D013212:Staphylococcus epidermidis / Q000821:virology; D014764:Viral Proteins / Q000737:chemistry*
chemical_list: D021122:Protein Subunits; D011994:Recombinant Proteins; D014764:Viral Proteins