title: Characterization and analytical application of Morin - bovine serum albumin system by spectroscopic approaches.
abstract: It is found that the fluorescence intensity of Morin can be strongly quenched by proteins. Based on this, a new fluorimetric method for the determination of protein was developed. Under optimum conditions, the quenchment of Morin fluorescence was in proportion to the concentration of proteins in the range 0.0001-0.1000 g·L(-1) for bovine serum albumin (BSA) and 0.0005-0.1000 g·L(-1) for human serum albumin (HSA). The reaction mechanism indicates that proteins can bind with Morin at the 3-hydroxyl and the 4-carbonyl and form a non-fluorescence complex 4:1 molar ratio of Morin/BSA, which results in the fluorescence of Morin and BSA are all quenched.
authors.affiliation: Department of Chemistry and Chemical Engineering, Zaozhuang University, Zaozhuang 277160, PR China. wf3786621@163.com
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Miao
authors.forename: Bo
authors.initials: B
authors.lastname: Tang
mesh_terms: D000818:Animals; D002417:Cattle; D005419:Flavonoids / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods*; D047309:Flavones
chemical_list: D005419:Flavonoids; D007202:Indicators and Reagents; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; C008548:morin; D047309:Flavones