title: Kinetic studies of L-asparaginase from Penicillium digitatum.
abstract: L-Asparaginase is an enzyme used in the treatment of acute lymphoblastic leukemia and other related malignancies. Its further use includes reduction of asparagine concentration in food products, which may lead to formation of acrylamide. Currently bacterial asparaginase is produced at industrial scale, but the enzyme isolated from bacterial origin is often associated with adverse reactions. These side effects require development of asparaginase from alternative sources. In the present study, Penicillium digitatum was explored for the production of extracellular L-asparaginase using modified Czapek-Dox media. The enzyme was purified about 60.95-fold and then kinetic study showed that the Km value of the enzyme was 1 × 10⁻⁵ M. The optimum pH and temperature for the enzyme were 7.0 and 30°C, respectively. The optimum incubation period for L-asparaginase was 15 min. This work concludes that this enzyme can be a suitable candidate due to its strong kinetic properties, and further research can usher into development of asparaginase formulation from fungal origin with less adverse effects.
authors.affiliation: Department of Biotechnology, College of Life Sciences, Cancer Hospital & Research Institute, Gwalior-MP, 474009 India. abhi.shri76@gmail.com
authors.forename: Abhinav
authors.initials: A
authors.lastname: Shrivastava
authors.forename: Abdul Arif
authors.initials: AA
authors.lastname: Khan
authors.forename: Archana
authors.initials: A
authors.lastname: Shrivastav
authors.forename: Sudhir K
authors.initials: SK
authors.lastname: Jain
authors.forename: Pradeep K
authors.initials: PK
authors.lastname: Singhal
mesh_terms: D001215:Asparaginase / Q000737:chemistry / Q000302:isolation & purification*; D001216:Asparagine / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D004789:Enzyme Activation; D057075:Enzyme Assays; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology*; D013696:Temperature; D013997:Time Factors
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D001216:Asparagine; D001215:Asparaginase