title: Bioefficacy of EPA-DHA from lipids recovered from fish processing wastes through biotechnological approaches.
abstract: The effect of fish oil recovered from fish visceral waste (FVW-FO) on serum and liver lipids, activity of HMG-CoA reductase in liver microsomes and EPA+DHA incorporation in liver, heart and brain were evaluated. Rats were fed different concentrations of FVW-FO providing 1.25%, 2.50%, 5.0% EPA+DHA recovered by either fermentation or enzymatic hydrolysis for 8weeks. Feeding FVW-FO reduce triacylglycerols (5.96-20.3%), total cholesterol (7.9-21.5%) and LDL (7.39-21.7%) cholesterol levels in serum compared to group fed on a control diet (groundnut oil). The activity of HMG-CoA reductase was reduced (p<0.05) in the FVW-FO fed groups compared to the control. EPA+DHA level in serum, liver, brain and heart increased with increments in dietary EPA+DHA. Results show the hypolipidemic property of FVW-FO and reduced HMG-CoA reductase activity which is proportional to the incorporation of EPA+DHA. Recovery of FVW-FO will address the increasing demand for fish oil and reduce pollution problems.
authors.affiliation: Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Rai
authors.forename: N
authors.initials: N
authors.lastname: Bhaskar
authors.forename: V
authors.initials: V
authors.lastname: Baskaran
mesh_terms: D000818:Animals; D001709:Biotechnology; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000008:administration & dosage* / Q000302:isolation & purification; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage* / Q000302:isolation & purification; D005395:Fish Oils / Q000008:administration & dosage / Q000032:analysis*; D005399:Fishes; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014866:Waste Products / Q000032:analysis*
chemical_list: D005395:Fish Oils; D008055:Lipids; D014866:Waste Products; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid