title: Early otic development depends on autophagy for apoptotic cell clearance and neural differentiation.
abstract: Autophagy is a highly regulated program of self-degradation of the cytosolic constituents that has key roles during early development and in adult cell growth and homeostasis. To investigate the role of autophagy in otic neurogenesis, we studied the expression of autophagy genes in early stages of chicken (Gallus gallus) inner ear development and the consequences of inhibiting the autophagic pathway in organotypic cultures of explanted chicken otic vesicles (OVs). Here we show the expression of autophagy-related genes (Atg) Beclin-1 (Atg6), Atg5 and LC3B (Atg8) in the otocyst and the presence of autophagic vesicles by using transmission electron microscopy in the otic neurogenic zone. The inhibition of the transcription of LC3B by using antisense morpholinos and of class III phosphatidylinositol 3-kinase with 3-methyladenine causes an aberrant morphology of the OV with accumulation of apoptotic cells. Moreover, inhibition of autophagy provokes the misregulation of the cell cycle in the otic epithelium, impaired neurogenesis and poor axonal outgrowth. Finally, our results indicate that autophagy provides the energy required for the clearing of neuroepithelial dying cells and suggest that it is required for the migration of otic neuronal precursors. Taken together, our results show for the first time that autophagy is an active and essential process during early inner ear development.
authors.affiliation: Instituto de Investigaciones Biomédicas 'Alberto Sols', CSIC-UAM, Madrid, Spain.
authors.forename: M R
authors.initials: MR
authors.lastname: Aburto
authors.forename: H
authors.initials: H
authors.lastname: Sánchez-Calderón
authors.forename: J M
authors.initials: JM
authors.lastname: Hurlé
authors.forename: I
authors.initials: I
authors.lastname: Varela-Nieto
authors.forename: M
authors.initials: M
authors.lastname: Magariños
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001343:Autophagy; D002478:Cells, Cultured; D002645:Chickens; D007758:Ear, Inner / Q000254:growth & development / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D060172:Morpholinos / Q000494:pharmacology; D046569:Neuroepithelial Cells / Q000166:cytology / Q000378:metabolism; D055495:Neurogenesis; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism; D000081082:Phosphoinositide-3 Kinase Inhibitors; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D008869:Microtubule-Associated Proteins; D060172:Morpholinos; D000081082:Phosphoinositide-3 Kinase Inhibitors; C025946:3-methyladenine; D058539:Phosphatidylinositol 3-Kinase; D000225:Adenine