title: Reverting antibiotic tolerance of Pseudomonas aeruginosa PAO1 persister cells by (Z)-4-bromo-5-(bromomethylene)-3-methylfuran-2(5H)-one.
abstract: BACKGROUND
  Bacteria are well known to form dormant persister cells that are tolerant to most antibiotics. Such intrinsic tolerance also facilitates the development of multidrug resistance through acquired mechanisms. Thus persister cells are a promising target for developing more effective methods to control chronic infections and help prevent the development of multidrug-resistant bacteria. However, control of persister cells is still an unmet challenge.
  
  
  RESULTS
  We show in this report that (Z)-4-bromo-5-(bromomethylene)-3-methylfuran-2(5H)-one (BF8) can restore the antibiotic susceptibility of Pseudomonas aeruginosa PAO1 persister cells at growth non-inhibitory concentrations. Persister control by BF8 was found to be effective against both planktonic and biofilm cells of P. aeruginosa PAO1. Interestingly, although BF8 is an inhibitor of quorum sensing (QS) in Gram-negative bacteria, the data in this study suggest that the activities of BF8 to revert antibiotic tolerance of P. aeruginosa PAO1 persister cells is not through QS inhibition and may involve other targets.
  
  
  CONCLUSIONS
  BF8 can sensitize P. aeruginosa persister cells to antibiotics.
authors.affiliation: Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA.
authors.forename: Jiachuan
authors.initials: J
authors.lastname: Pan
authors.forename: Ali Adem
authors.initials: AA
authors.lastname: Bahar
authors.forename: Haseeba
authors.initials: H
authors.lastname: Syed
authors.forename: Dacheng
authors.initials: D
authors.lastname: Ren
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000704:Analysis of Variance; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D002228:Carbenicillin / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004357:Drug Synergism; D005798:Genes, Bacterial; D005839:Gentamicins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D020411:Oligonucleotide Array Sequence Analysis; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D053038:Quorum Sensing / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology; D014031:Tobramycin / Q000494:pharmacology; D059467:Transcriptome / Q000187:drug effects
chemical_list: C578511:4-bromo-5-(bromomethylene)-3-methylfuran-2(5H)-one; D000900:Anti-Bacterial Agents; D005839:Gentamicins; D002939:Ciprofloxacin; D013752:Tetracycline; D002228:Carbenicillin; D015107:4-Butyrolactone; D014031:Tobramycin