title: Unexpected dissemination patterns in lymphoma progression revealed by serial imaging within a murine lymph node.
abstract: Non-Hodgkin lymphoma (NHL) is a heterogeneous and highly disseminated disease, but the mechanisms of its growth and dissemination are not well understood. Using a mouse model of this disease, we used multimodal imaging, including intravital microscopy (IVM) combined with bioluminescence, as a powerful tool to better elucidate NHL progression. We injected enhanced green fluorescent protein and luciferase-expressing Eμ-Myc/Arf(-/-) (Cdkn2a(-/-)) mouse lymphoma cells (EL-Arf(-/-)) into C57BL/6NCrl mice intravenously. Long-term observation inside a peripheral lymph node was enabled by a novel lymph node internal window chamber technique that allows chronic, sequential lymph node imaging under in vivo physiologic conditions. Interestingly, during early stages of tumor progression we found that few if any lymphoma cells homed initially to the inguinal lymph node (ILN), despite clear evidence of lymphoma cells in the bone marrow and spleen. Unexpectedly, we detected a reproducible efflux of lymphoma cells from spleen and bone marrow, concomitant with a massive and synchronous influx of lymphoma cells into the ILN, several days after injection. We confirmed a coordinated efflux/influx of tumor cells by injecting EL-Arf(-/-) lymphoma cells directly into the spleen and observing a burst of lymphoma cells, validating that the burst originated in organs remote from the lymph nodes. Our findings argue that in NHL an efflux of tumor cells from one disease site to another, distant site in which they become established occurs in discrete bursts.
authors.affiliation: Molecular Imaging Program, Department of Radiology, Stanford University, Stanford, California, USA.
authors.forename: Ken
authors.initials: K
authors.lastname: Ito
authors.forename: Bryan Ronain
authors.initials: BR
authors.lastname: Smith
authors.forename: Natesh
authors.initials: N
authors.lastname: Parashurama
authors.forename: Joon-Kee
authors.initials: JK
authors.lastname: Yoon
authors.forename: Si Yeol
authors.initials: SY
authors.lastname: Song
authors.forename: Cornelius
authors.initials: C
authors.lastname: Miething
authors.forename: Parag
authors.initials: P
authors.lastname: Mallick
authors.forename: Scott
authors.initials: S
authors.lastname: Lowe
authors.forename: Sanjiv Sam
authors.initials: SS
authors.lastname: Gambhir
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D018450:Disease Progression; D008163:Luminescent Measurements; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology*; D008207:Lymphatic Metastasis; D008228:Lymphoma, Non-Hodgkin / Q000000981:diagnostic imaging / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011859:Radiography; D013154:Spleen / Q000378:metabolism / Q000473:pathology