title: Preparative separation of glycoalkaloids α-solanine and α-chaconine by centrifugal partition chromatography.
abstract: The main glycoalkaloids of a commercial potato cultivar, α-chaconine and α-solanine, were extracted from sprouts of Solanum tuberosum cv. Pompadour by a mixture of MeOH/H(2)O/CH(3)COOH (400/100/50, v/v/v). In these conditions, 2.8±0.62g of crude extract were obtained from 50g of fresh sprouts and the total glycoalkaloid content was determined by analytical HPLC at 216.5mg/100g. α-Chaconine and α-solanine were separated in a preparative scale using centrifugal partition chromatography (CPC). In a solvent system composed of a mixture of ethyl acetate/butanol/water (15/35/50, v/v/v), α-chaconine (54mg) and α-solanine (15mg) were successfully isolated from the crude extract in one step of purification. The purity of isolated compounds was determined to be higher than 92% by HPLC analysis.
authors.affiliation: S.I.P.R.E - Comité Nord, rue des champs Potez, 62217 Achicourt, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Attoumbré
authors.forename: David
authors.initials: D
authors.lastname: Lesur
authors.forename: Philippe
authors.initials: P
authors.lastname: Giordanengo
authors.forename: Sylvie
authors.initials: S
authors.lastname: Baltora-Rosset
mesh_terms: D002498:Centrifugation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009930:Organic Chemicals / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D012992:Solanine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D009930:Organic Chemicals; D010936:Plant Extracts; C514919:alpha-solanine; D014867:Water; D012992:Solanine; C011860:alpha-chaconine