title: Cell death detection by quantitative three-dimensional single-cell tomography.
abstract: Ultrahigh-resolution optical coherence tomography (UR-OCT) has been used for the first time to our knowledge to study single-cell basal cell carcinoma (BCC) in vitro. This noninvasive, in situ, label-free technique with deep imaging depth enables three-dimensional analysis of scattering properties of single cells with cellular spatial resolution. From three-dimensional UR-OCT imaging, live and dead BCC cells can be easily identified based on morphological observation. We developed a novel method to automatically extract characteristic parameters of a single cell from data volume, and quantitative comparison and parametric analysis were performed. The results demonstrate the capability of UR-OCT to detect cell death at the cellular level.
authors.affiliation: Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei,  Taiwan.
authors.forename: Nai-Chia
authors.initials: NC
authors.lastname: Cheng
authors.forename: Tsung-Hsun
authors.initials: TH
authors.lastname: Hsieh
authors.forename: Yu-Ta
authors.initials: YT
authors.lastname: Wang
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Lai
authors.forename: Chia-Kai
authors.initials: CK
authors.lastname: Chang
authors.forename: Ming-Yi
authors.initials: MY
authors.lastname: Lin
authors.forename: Ding-Wei
authors.initials: DW
authors.lastname: Huang
authors.forename: Jeng-Wei
authors.initials: JW
authors.lastname: Tjiu
authors.forename: Sheng-Lung
authors.initials: SL
authors.lastname: Huang