title: Antiherpetic activity of an Agaricus brasiliensis polysaccharide, its sulfated derivative and fractions.
abstract: Agaricus brasiliensis is an edible mushroom, traditionally used for the treatment of several diseases. In this paper, a polysaccharide (PLS) from A. brasiliensis, its carboxymethylated (CPLS) and sulfated (SPLS) derivatives, as well as, fractions (F1-F3) obtained from the PLS were investigated for their effect in the replication of herpes simplex virus and bovine herpes virus in HEp-2 cell cultures. The PLS, SPLS and F3 inhibited both virus strains similarly, in a dose-dependent curve. F1, F2 and CPLS did not show significant effect even at higher concentrations. All the compounds showed neither virucidal or viral adsorption inhibition activities nor effect when cells were treated prior to infection. Our study demonstrated that the extracts of A. brasiliensis, can be promising for future antiviral drug design and its biotechnological production is economically feasible.
authors.affiliation: Departamento de Microbiologia, CCB, Universidade Estadual de Londrina, Londrina, Paraná, Brazil.
authors.forename: Kristie Aimi
authors.initials: KA
authors.lastname: Yamamoto
authors.forename: Lígia Carla Faccin
authors.initials: LC
authors.lastname: Galhardi
authors.forename: Vinícius Pires
authors.initials: VP
authors.lastname: Rincão
authors.forename: Sandra de Aguiar
authors.initials: Sde A
authors.lastname: Soares
authors.forename: Icaro Gusmão Pinto
authors.initials: IG
authors.lastname: Vieira
authors.forename: Nágila Maria Pontes Silva
authors.initials: NM
authors.lastname: Ricardo
authors.forename: Carlos
authors.initials: C
authors.lastname: Nozawa
authors.forename: Rosa Elisa Carvalho
authors.initials: RE
authors.lastname: Linhares
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology*; D002417:Cattle; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D006561:Herpes Simplex / Q000188:drug therapy* / Q000378:metabolism; D007242:Herpesvirus 1, Bovine / Q000378:metabolism*; D006801:Humans; D018139:Simplexvirus / Q000378:metabolism*
chemical_list: D000998:Antiviral Agents; D062610:Fungal Polysaccharides