title: NIa-pro of Papaya ringspot virus interacts with papaya methionine sulfoxide reductase B1.
abstract: A chloroplast-localized papaya methionine sulfoxide reductase B1 (PaMsrB1) interacting with Papaya ringspot virus (PRSV) NIa-Pro was identified using a Sos recruitment two-hybrid system (SRS). SRS analysis of several deletion mutants of PRSV NIa-Pro and PaMsrB1 demonstrated that the C-terminal (residues 133-239) fragment of PRSV NIa-Pro and residues 112-175 of PaMsrB1 were necessary for this interaction between PRSV NIa-Pro and PaMsrB1. MsrB1 can repair Met-oxidized proteins damaged by reactive oxygen species (ROS). We confirmed that PRSV infection leads to ROS accumulation and a slight upregulation of level PaMsrB1 mRNA in papaya. This interaction between PaMsrB1 with PRSV NIa-Pro may disturb the import of PaMsrB1 into the chloroplasts. These results suggest that this specific interaction could interfere with PaMsrB1 into the chloroplasts to scavenge ROS caused by PRSV infection. This may be a novel mechanism of PRSV towards the host defense.
authors.affiliation: Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology & Analysis and Testing Center, Chinese Academy of Tropical Agricultural Science, Haikou 571101, People's Republic of China.
authors.forename: Le
authors.initials: L
authors.lastname: Gao
authors.forename: Wentao
authors.initials: W
authors.lastname: Shen
authors.forename: Pu
authors.initials: P
authors.lastname: Yan
authors.forename: Decai
authors.initials: D
authors.lastname: Tuo
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
mesh_terms: D000595:Amino Acid Sequence; D029441:Carica / Q000821:virology*; D010450:Endopeptidases / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D051150:Methionine Sulfoxide Reductases / Q000378:metabolism*; D008969:Molecular Sequence Data; D017800:Potyvirus / Q000201:enzymology* / Q000472:pathogenicity*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D051150:Methionine Sulfoxide Reductases; D010450:Endopeptidases; C059992:nuclear inclusion protein a, mosaic viruses