title: Synthesis of polyethylene glycol- and sulfobetaine-conjugated zwitterionic poly(L-lactide) and assay of its antifouling properties.
abstract: A new antifouling polyester monomethoxy-poly(ethylene glycol)-b-poly(L-lactide)-b-poly(sulfobetaine methacrylate) (MPEG-PLA-PSBMA) was obtained by ring-opening polymerization of L-lactide, and subsequent click chemistry to graft the azide end-functionalized poly(sulfobetaine methacrylate) (polySBMA) moieties onto the alkyne end-functionalized MPEG-PLA (MPEG-PLA-alkyne). The chemical structure of the polymer was characterized using (1)H nuclear magnetic resonance and Fourier-transform infrared spectroscopy, and its physical properties (including molecular weight, glass transition temperature, and melting point) were determined using gel permeation chromatography and differential scanning calorimetry. To investigate its hydrophilicity and stability, as well as its antifouling properties, the polymer was also prepared as a surface coating on glass substrates. The wettability and stability of this polyester was examined by contact angle measurements. Furthermore, its antifouling properties were investigated via protein adsorption, cell adhesion studies, and bacterial attachment assays. The results suggest that the prepared zwitterionic polyester exhibits durable wettability and stability, as well as significant antifouling properties. The new zwitterionic polyester MPEG-PLA-PSBMA could be developed as a promising antifouling material with extensive biomedical applications.
authors.affiliation: College of Science, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Tu
authors.forename: Jian-Chun
authors.initials: JC
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Liu
authors.forename: Mao-Sen
authors.initials: MS
authors.lastname: Yuan
authors.forename: Wenming
authors.initials: W
authors.lastname: Liu
authors.forename: Jinyi
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D057930:Click Chemistry; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000009:adverse effects / Q000138:chemical synthesis* / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D011091:Polyesters; D011108:Polymers; D001622:Betaine; D011092:Polyethylene Glycols; C033616:poly(lactide); C483727:sulfobetaine