title: Regulation of mean and noise of the in vivo kinetics of transcription under the control of the lac/ara-1 promoter.
abstract: The kinetics of transcription initiation in Escherichia coli depend on the duration of two rate-limiting steps, the closed and the open complex formation. In a lac promoter variant, P(lac/ara-1), the kinetics of these steps is controlled by IPTG and arabinose. From in vivo single-RNA measurements, we find that induction affects the mean and normalized variance of the intervals between consecutive RNA productions. Transcript production is sub-Poissonian in all conditions tested. The kinetics of each step is independently controlled by a different inducer. We conclude that the regulatory mechanism of P(lac/ara-1) allows the stochasticity of gene expression to be environment-dependent.
authors.affiliation: Laboratory of Biosystem Dynamics, Department of Signal Processing, Tampere University of Technology, 33101 Tampere, Finland.
authors.forename: Meenakshisundaram
authors.initials: M
authors.lastname: Kandhavelu
authors.forename: Jason
authors.initials: J
authors.lastname: Lloyd-Price
authors.forename: Abhishekh
authors.initials: A
authors.lastname: Gupta
authors.forename: Anantha-Barathi
authors.initials: AB
authors.lastname: Muthukrishnan
authors.forename: Olli
authors.initials: O
authors.lastname: Yli-Harja
authors.forename: Andre S
authors.initials: AS
authors.lastname: Ribeiro
mesh_terms: D001089:Arabinose / Q000494:pharmacology; D001483:Base Sequence; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D007700:Kinetics; D007763:Lac Operon; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012313:RNA / Q000096:biosynthesis / Q000235:genetics*; D061785:Transcription Initiation, Genetic / Q000187:drug effects; D015533:Transcriptional Activation
chemical_list: D007544:Isopropyl Thiogalactoside; D012313:RNA; D001089:Arabinose