title: Embryonic stem (ES) cell culture basics.
abstract: This unit describes the procedures to grow and maintain embryonic stem (ES) cells in culture. Growth-inactivated mouse embryo fibroblasts (MEF) are required as a feeder layer for the ES cells; their isolation and culture is described as are the additional protocols required for generating a mouse with a particular targeted gene electroporation, cloning and selection for homologous recombinants, transfection with Cre, and preparation of cells for injection.
authors.affiliation: University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: J V
authors.initials: JV
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003470:Culture Media; D018274:Electroporation; D053595:Embryonic Stem Cells / Q000166:cytology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008845:Microinjections; D014162:Transfection
chemical_list: D003470:Culture Media