title: Carbohydrate immobilized on a dendrimer-coated colloidal gold surface for fabrication of a lectin-sensing device based on localized surface plasmon resonance spectroscopy.
abstract: Carbohydrate-mediated functions in biological systems have generated considerable interest in recent years. We have developed a device bearing immobilized carbohydrates on a colloidal gold surface and applied this device to the detection of carbohydrate-binding molecules by using localized surface plasmon resonance (LSPR) spectroscopy. The sensing device was constructed by using cyanuric chloride as an amine-linker between an amino residue of a polyamidoamine (PAMAM) dendrimer-coated colloidal gold surface and the amino residue of a 12-aminododecyl glycoside. After optimizing the construction of the device, we characterized its LSPR-based sensing capability. Binding specificity with lectins and linear range responses were obtained with the device. Our LSPR-based sensing device thus provides a label-free, low-cost detection method for use as a laboratory research tool or in medical glycan arrays.
authors.affiliation: Japan Chemical Innovation Institute, 1-3-5 Kanda-jimbocho, Tokyo 101-0051, Japan.
authors.forename: Masayo
authors.initials: M
authors.lastname: Ogiso
authors.forename: Junko
authors.initials: J
authors.lastname: Kobayashi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Imai
authors.forename: Koji
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Miki
authors.initials: M
authors.lastname: Itoh
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imamura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Okada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miura
authors.forename: Toshinori
authors.initials: T
authors.lastname: Nishiyama
authors.forename: Kenichi
authors.initials: K
authors.lastname: Hatanaka
authors.forename: Norihiko
authors.initials: N
authors.lastname: Minoura
mesh_terms: D001665:Binding Sites; D015374:Biosensing Techniques / Q000295:instrumentation*; D002241:Carbohydrates / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry; D050091:Dendrimers / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry*; D037102:Lectins / Q000032:analysis* / Q000737:chemistry; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000295:instrumentation*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*
chemical_list: D002241:Carbohydrates; D003102:Colloids; D050091:Dendrimers; D037102:Lectins; C104700:PAMAM Starburst; D006046:Gold