title: Protective effect of planarian DJ-1 against 6-hydroxydopamine-induced neurotoxicity.
abstract: DJ-1/PARK7 has multiple functions as an antioxidant, an oncogene, and a molecular chaperone in vertebrates, and loss-of-function mutations in DJ-1 cause early onset of Parkinson's disease. However, the function of invertebrate DJ-1 remains unknown. In order to investigate the function of planarian DJ-1, we isolated the planarian DJ-1 gene Dugesia japonica DJ-1 (DjDJ-1) and analyzed its expression and function. In situ hybridization analysis revealed that DjDJ-1 mRNA was expressed throughout the body, including the central nervous system, cells surrounding the pharynx, and stem cells. Planarian DjDJ-1 protein exhibited antioxidant function, similar to human DJ-1, as evidenced by the fact that recombinant DjDJ-1 protein reduced reactive oxygen species and protected human neuroblastoma SH-SY5Y cells from cell death. In addition, dopaminergic neurons in DjDJ-1(RNAi) planarians became susceptible to 6-hydroxydopamine, a dopaminergic neurotoxin. These results suggest that planarians have a DJ-1 ortholog, which has conserved antioxidant and neuroprotective functions.
authors.affiliation: Department of Clinical and Translational Physiology, Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Tsushima
authors.forename: Kaneyasu
authors.initials: K
authors.lastname: Nishimura
authors.forename: Natsuka
authors.initials: N
authors.lastname: Tashiro
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Takata
authors.forename: Eishi
authors.initials: E
authors.lastname: Ashihara
authors.forename: Kanji
authors.initials: K
authors.lastname: Yoshimoto
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ariga
authors.forename: Kiyokazu
authors.initials: K
authors.lastname: Agata
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Kitamura
mesh_terms: D018663:Adrenergic Agents / Q000633:toxicity; D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D059290:Dopaminergic Neurons / Q000187:drug effects / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D016627:Oxidopamine / Q000633:toxicity; D010932:Planarians / Q000235:genetics* / Q000378:metabolism*; D000071617:Protein Deglycase DJ-1; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
chemical_list: D018663:Adrenergic Agents; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D016627:Oxidopamine; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1