title: Altered regional blood flow distribution in Mas-deficient mice.
abstract: BACKGROUND
  We have recently shown that the acute infusion of angiotensin-(1-7) [Ang-(1-7)] or chronic increase in plasma Ang-(1-7) produces important changes in regional blood flow in rats.
  
  
  METHODS
  To further assess whether these changes are related to Mas, in this study hemodynamic measurements were performed in Ang-(1-7) receptor Mas knockout C57BL/6 (Mas-KO) mice and age-matched wild type (WT) control mice, using fluorescent microspheres.
  
  
  RESULTS
  Mean arterial pressure in urethane-anesthetized Mas-KO mice (12-16 weeks old) did not differ from that in WT mice (79 ± 2 and 80 ± 2 mmHg respectively). However, pronounced differences were observed in other hemodynamic measurements. Mas-KO mice exhibited a significant decrease in stroke volume (0.03 ± 0.01 versus 0.05 ± 0.01 ml/beat in WT) and decreased cardiac index (0.81 ± 0.08 versus 1.24 ± 0.24 ml/min/g in WT). Strikingly, Mas-KO mice exhibited a marked increase in vascular resistance and a decrease in blood flow in the kidney, lung, adrenal gland, mesentery, spleen and brown fat tissue. The decrease in blood flow ranged from 34% (spleen) to 55% (brown fat tissue).
  
  
  CONCLUSIONS
  These results suggest that the Ang-(1-7)/Mas axis plays an important role in regional and systemic hemodynamic adjustments in mice.
authors.affiliation: National Institute of Science and Technology in Nanobiopharmaceutics, Department of Physiology and Biophysics, ICBUFMG, Minas Gerais, Brazil.
authors.forename: Giancarla Aparecida
authors.initials: GA
authors.lastname: Botelho-Santos
authors.forename: Michael
authors.initials: M
authors.lastname: Bader
authors.forename: Nathalia
authors.initials: N
authors.lastname: Alenina
authors.forename: Robson Augusto Souza
authors.initials: RA
authors.lastname: Santos
mesh_terms: D000818:Animals; D062186:Arterial Pressure / Q000502:physiology*; D005453:Fluorescence; D006439:Hemodynamics / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008863:Microspheres; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D012039:Regional Blood Flow / Q000502:physiology*; D013318:Stroke Volume / Q000502:physiology*; D014520:Urethane / Q000008:administration & dosage; D014655:Vascular Resistance / Q000502:physiology
chemical_list: D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D043562:Receptors, G-Protein-Coupled; D014520:Urethane