title: Lens-less surface second harmonic imaging.
abstract: Lens-less surface second harmonic generation imaging (SSHGI) is used to image an SHG active molecule, (S)-(+)-1,1'-bi-2-naphthol (SBN), incorporated into a lipid bilayer patterned with the 1951 United States Air Force resolution test target. Data show the coherent plane-wave nature of SHG allows direct imaging without the aid of a lens system. Lens-less SSHGI readily resolves line-widths as small as 223 μm at an object-image distance of 7.6 cm and line-widths of 397 μm at distances as far as 30 cm. Lens-less SSHGI simplifies the detection method, raises photon collection efficiency, and expands the field-of-view. These advantages allow greater throughput and make lens-less SSHGI a potentially valuable detection method for biosensors and medical diagnostics.
authors.affiliation: Department of Chemistry, University of Utah, 315 South 1400 East, Rm 2020, Salt Lake City, Ut 84112, USA.
authors.forename: Krystal L
authors.initials: KL
authors.lastname: Sly
authors.forename: Trang T
authors.initials: TT
authors.lastname: Nguyen
authors.forename: John C
authors.initials: JC
authors.lastname: Conboy
mesh_terms: D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D008027:Light; D008029:Lighting / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D012542:Scattering, Radiation