title: Analysis of human mesenchymal stem cells on a cross-linked collagen-based surgical implant material.
abstract: Biomimetic scaffolds, delivered to the patient with or without cell loading, can offer improved treatments for a range of damaged or diseased tissues. In this work we evaluated the capacity of a cross-linked collagen-based implant material (Permacol™) to support the survival and growth of multipotent stromal cells or mesenchymal stem cells (MSCs) for potential applications in soft-tissue reconstruction and compared it to uncross-linked collagen. We showed, using confocal imaging, histology, quantitative analysis of seeding efficiencies by the MTT assay and Live/Dead analysis, that Permacol™ supported the adherence, growth and survival of MSCs preserving a healthy cell phenotype. MSCs infiltrated both matrices, although migration rates were significantly higher on uncross-linked matrices for some donors. Our work demonstrates that Permacol™ could maintain human MSCs in long-term culture supporting its use as a cell-seeded biomaterial for durable and strong repair in tissue reconstruction.
authors.affiliation: Department of Biology, University of York, York, UK.
authors.forename: Turkan
authors.initials: T
authors.lastname: Arca
authors.forename: Joanne
authors.initials: J
authors.lastname: Proffitt
authors.forename: Paul
authors.initials: P
authors.lastname: Genever
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D005109:Extracellular Matrix / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D019736:Prostheses and Implants; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D003094:Collagen