title: Evaluation of bone regeneration using the rat critical size calvarial defect.
abstract: Animal models that are reliably reproducible, appropriate analogs to the clinical condition they are used to investigate, and that offer minimal morbidity and periprocedural mortality to the subject, are the keystone to the preclinical development of translational technologies. For bone tissue engineering, a number of small animal models exist. Here we describe the protocol for one such model, the rat calvarial defect. This versatile model allows for evaluation of biomaterials and bone tissue engineering approaches within a reproducible, non-load-bearing orthotopic site. Crucial steps for ensuring appropriate experimental control and troubleshooting tips learned through extensive experience with this model are provided. The surgical procedure itself takes ∼30 min to complete, with ∼2 h of perioperative care, and tissue collection is generally performed 4-12 weeks postoperatively. Several analytical techniques are presented, which evaluate the cellular and extracellular matrix components, functionality and mineralization, including histological, mechanical and radiographic methods.
authors.affiliation: Department of Bioengineering, Rice University, Houston, Texas, USA.
authors.forename: Patrick P
authors.initials: PP
authors.lastname: Spicer
authors.forename: James D
authors.initials: JD
authors.lastname: Kretlow
authors.forename: Simon
authors.initials: S
authors.lastname: Young
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.forename: F Kurtis
authors.initials: FK
authors.lastname: Kasper
authors.forename: Antonios G
authors.initials: AG
authors.lastname: Mikos
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D023421:Models, Animal; D051381:Rats; D011916:Rats, Inbred F344; D015203:Reproducibility of Results; D012886:Skull / Q000502:physiology* / Q000601:surgery; D023822:Tissue Engineering / Q000379:methods*