title: Cellular assessment of the extract of bambangan (Mangifera pajang) as a potential cytoprotective agent for the human hepatocellular HepG2 cell line.
abstract: This study was conducted to investigate the potential of bambangan (Mangifera pajang) fruit extracts in the protection against oxidative damage caused by tert-butyl hydroperoxide in the human hepatocellular HepG2 cell line. Proteins which might be involved in the cytoprotective mechanism were investigated using western blotting technique. Quercetin was used as a positive control. The results showed that only the kernel extract of M. pajang and quercetin displayed cytoprotective activity in HepG2 cells, with EC(50) values of 1.2 and 5.3μg/ml, respectively. Expression of quinone reductase, glutathione reductase and methionine sulfoxide reductase A proteins were significantly up-regulated by quercetin, suggesting their involvement in the cytoprotective activity of quercetin. However, expressions of only glutathione reductase and methionine sulfoxide reductase A proteins were significantly up-regulated by the kernel extract, again suggesting their involvement in the cytoprotective activity of bambangan kernel extract. Future study is needed to investigate the involvement of other cytoprotective proteins in the cytoprotection mechanism.
authors.affiliation: Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Sabah, Malaysia. mofadz@ums.edu.my
authors.forename: Mohd Fadzelly
authors.initials: MF
authors.lastname: Abu Bakar
authors.forename: Maryati
authors.initials: M
authors.lastname: Mohamed
authors.forename: Asmah
authors.initials: A
authors.lastname: Rahmat
authors.forename: Steven A
authors.initials: SA
authors.lastname: Burr
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Fry
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D005980:Glutathione Reductase / Q000235:genetics / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D031022:Mangifera / Q000737:chemistry*; D051150:Methionine Sulfoxide Reductases / Q000235:genetics / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D011794:Quercetin; D016660:NAD(P)H Dehydrogenase (Quinone); D005980:Glutathione Reductase; D051150:Methionine Sulfoxide Reductases