title: Influence of DNA-repair gene variants on the micronucleus frequency in thyroid cancer patients.
abstract: The role of different DNA-repair genes (OGG1, XRCC1, XRCC2 and XRCC3) on both the spontaneous and the induced frequency of micronuclei (MN) has been studied in the lymphocytes of a group of 114 patients with differentiated thyroid cancer (DTC). Induction of MN was achieved by treatment of the lymphocytes with 0.5Gy of gamma-radiation. The selected genes are involved in base-excision repair (BER) (OGG1, Ser326Cys; XRCC1, Arg280His and Arg399Gln), and in homologous recombination repair (HRR) (XRCC2, Arg188His and XRCC3, IVS5-14G). Genotyping was carried out by use of the iPLEX (Sequenom) technique. Results indicate that only the OGG1-Ser326Cys polymorphism was able to modulate the MN frequency. This effect was only observed in the spontaneous MN frequency (P=0.016), but not in the MN frequency induced after irradiation. In addition, a strong correlation was observed between spontaneous and induced MN frequency, which would suggest an underlying genetic background.
authors.affiliation: Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: W A
authors.initials: WA
authors.lastname: García-Quispes
authors.forename: S
authors.initials: S
authors.lastname: Pastor
authors.forename: P
authors.initials: P
authors.lastname: Galofré
authors.forename: F
authors.initials: F
authors.lastname: Biarnés
authors.forename: J
authors.initials: J
authors.lastname: Castell
authors.forename: A
authors.initials: A
authors.lastname: Velázquez
authors.forename: R
authors.initials: R
authors.lastname: Marcos
mesh_terms: D000328:Adult; D004249:DNA Damage; D045643:DNA Repair Enzymes / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D048629:Micronuclei, Chromosome-Defective; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013964:Thyroid Neoplasms / Q000235:genetics*
chemical_list: D045643:DNA Repair Enzymes