title: Leukocyte, red blood cell and morphological adaptation to moderate physical training in rats undernourished in the neonatal period.
abstract: OBJECTIVE
  To analyze the impact of moderate physical exercise on the total and differential leukocyte counts and red blood cell count of 36 sixty-day-old adult male Wistar rats subjected to early malnourishment.
  
  
  METHODS
  The rats were divided in nourished (N - casein 17%) and malnourished groups (M - casein 8%) and thesegroups were then subdivided in trained (T) untrained (U) creating four groups NT, NU, MT and MU. The NT and MTgroups were submitted to moderate physical exercise using a treadmill (60 min/day, 5 days/week for 8 weeks). Onthe 1st day, before the training started T0 and 24 hours after the last training day of the week (T1 until T8), a 1 mLaliquot of blood was collected from the animals' tails for analysis. The total leukocyte count was evaluated in a cellcounter with an electronic microscope. The cyanmethemoglobin technique was used to measure the hemoglobin level. The hematocrit values were determined as a percentage using the micro-hematocrit technique with a microcapillaryreader and a cell counter was used to determine the red blood cell count. The t-test was used for statistical analysis and a p-value < 0.05 was considered significant. Data are expressed as means ± standard deviation.
  
  
  RESULTS
  There was a significant difference in the total leukocyte count between the NT (9.1 ± 0.1) and MT groups (8.0 ± 0.1) from T1 and in neutrophils between the NT (22.1 ± 0.6) and MT groups (24.6 ± 1.8) from T7 (p < 0.05). There was no statistical significance in the hemoglobin, hematocrit and red blood cell count from T1.
  
  
  CONCLUSIONS
  According to the results of this study, moderate physical exercise seems to have induced physiologic adaptation in adult rats from T1.
authors.affiliation: Universidade Federal de Pernambuco - UFPE, Recife, PE, Brazil.
authors.forename: Marcelo Tavares
authors.initials: MT
authors.lastname: Viana
authors.forename: Manuella Cavalcanti
authors.initials: MC
authors.lastname: Perez
authors.forename: Valdenilson Ribeiro
authors.initials: VR
authors.lastname: Ribas
authors.forename: Gilberto de Freire
authors.initials: Gde F
authors.lastname: Martins
authors.forename: Célia Maria Machado Barbosa
authors.initials: CM
authors.lastname: de Castro