title: Next-generation sequencing and de novo assembly, genome organization, and comparative genomic analyses of the genomes of two Helicobacter pylori isolates from duodenal ulcer patients in India.
abstract: The prevalence of different H. pylori genotypes in various geographical regions indicates region-specific adaptations during the course of evolution. Complete genomes of H. pylori from countries with high infection burdens, such as India, have not yet been described. Herein we present genome sequences of two H. pylori strains, NAB47 and NAD1, from India. In this report, we briefly mention the sequencing and finishing approaches, genome assembly with downstream statistics, and important features of the two draft genomes, including their phylogenetic status. We believe that these genome sequences and the comparative genomics emanating thereupon will help us to clearly understand the ancestry and biology of the Indian H. pylori genotypes, and this will be helpful in solving the so-called Indian enigma, by which high infection rates do not corroborate the minuscule number of serious outcomes observed, including gastric cancer.
authors.affiliation: Pathogen Biology Laboratory, Department of Biotechnology, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, India.
authors.forename: Narender
authors.initials: N
authors.lastname: Kumar
authors.forename: Asish K
authors.initials: AK
authors.lastname: Mukhopadhyay
authors.forename: Rajashree
authors.initials: R
authors.lastname: Patra
authors.forename: Ronita
authors.initials: R
authors.lastname: De
authors.forename: Ramani
authors.initials: R
authors.lastname: Baddam
authors.forename: Sabiha
authors.initials: S
authors.lastname: Shaik
authors.forename: Jawed
authors.initials: J
authors.lastname: Alam
authors.forename: Suma
authors.initials: S
authors.lastname: Tiruvayipati
authors.forename: Niyaz
authors.initials: N
authors.lastname: Ahmed
mesh_terms: D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D004381:Duodenal Ulcer / Q000150:complications / Q000382:microbiology; D023061:Gene Order; D016680:Genome, Bacterial; D016481:Helicobacter Infections / Q000150:complications / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D014481:United States
chemical_list: D004269:DNA, Bacterial