title: Detection of covalent binding.
abstract: Immunochemical detection of xenobiotics covalently bound to cellular proteins can provide information about toxic mechanism and is more specific than the alternative radiochemical studies. Both immunoblotting and immunohistochemical methods are used to pinpoint the target protein(s) and to identify the tissue targets. Also included in this unit are protocols for synthesizing artificial antigens, immunizing suitable host species, and using noncompetitive and competitive ELISA assays to characterize the antibodies produced.
authors.affiliation: University of Connecticut, Storrs, Connecticut, USA.
authors.forename: M K
authors.initials: MK
authors.lastname: Bruno
authors.forename: S D
authors.initials: SD
authors.lastname: Cohen
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007106:Immune Sera / Q000032:analysis / Q000302:isolation & purification; D015151:Immunoblotting; D007150:Immunohistochemistry; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D015262:Xenobiotics / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity
chemical_list: D006241:Haptens; D007106:Immune Sera; D011506:Proteins; D015262:Xenobiotics