title: Stimulation of Escherichia coli DNA damage inducible DNA helicase DinG by the single-stranded DNA binding protein SSB.
abstract: Escherichia coli DNA damage inducible protein DinG is a superfamily II DNA helicase and is closely related to human DNA helicase XPD. Here, we report that E. coli single-stranded DNA binding protein (SSB) is able to form a stable protein complex with DinG and to stimulate the DinG DNA helicase activity. An SSB mutant that retains the single-stranded DNA binding activity but fails to form a protein complex with DinG becomes a potent inhibitor for the DinG DNA helicase, suggesting that E. coli wild-type SSB stimulates the DinG DNA helicase via specific protein-protein interaction.
authors.affiliation: Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
authors.forename: Zishuo
authors.initials: Z
authors.lastname: Cheng
authors.forename: Aimee
authors.initials: A
authors.lastname: Caillet
authors.forename: Binbin
authors.initials: B
authors.lastname: Ren
authors.forename: Huangen
authors.initials: H
authors.lastname: Ding
mesh_terms: D002850:Chromatography, Gel; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009154:Mutation; D010957:Plasmids; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C476430:SSB protein, E coli; C074432:dinG protein, E coli; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human