title: Study of L-arginine in intestinal lesions caused by ischemia-reperfusion in rats.
abstract: To examine whether treatment with L-arginine (ARG), a substrate of nitric oxide biosynthesis, attenuated intestinal dysfunction caused by ischemia (I) and reperfusion (R), we treated rats with ARG (100 mg/kg intravenously) or saline solution (SS) before 60 minutes of I produced by occlusion of the superior mesenteric artery and/or during 120 minutes of R. After I or I/R, we isolated 2-cm jejunal segments for mounting in an organ bath to study neurogenic contractions stimulated by electrical pulses or KCl with the use of a digital recording system. Thin jejunal slices were stained with hematoxylin and eosin for optical microscopy. Jejunal contractions were similar in the sham and I+ARG, but reduced in I+SS, I/R+SS, and I/R+ARG groups. Jejunal enteric nerves were damaged in I+SS, IR+SS, and IR+ARG, but not in the I+ARG group, suggesting that ARG attenuate intestinal dysfunctions due to I but not to R.
authors.affiliation: Department of Pharmacology, Federal University of São Paulo, São Paulo, Brazil.
authors.forename: J S M
authors.initials: JS
authors.lastname: Gomez
authors.forename: R
authors.initials: R
authors.lastname: Miranda-Ferreira
authors.forename: N S A
authors.initials: NS
authors.lastname: Taha
authors.forename: L W
authors.initials: LW
authors.lastname: Rodrigues
authors.forename: D R G M
authors.initials: DR
authors.lastname: Bento
authors.forename: D T
authors.initials: DT
authors.lastname: Fernandes
authors.forename: R T
authors.initials: RT
authors.lastname: Chihara
authors.forename: M J
authors.initials: MJ
authors.lastname: Simões
authors.forename: I S
authors.initials: IS
authors.lastname: Oliveira-Junior
authors.forename: H P
authors.initials: HP
authors.lastname: Monteiro
authors.forename: D J
authors.initials: DJ
authors.lastname: Fagundes
authors.forename: A
authors.initials: A
authors.lastname: Caricati-Neto
authors.forename: M O
authors.initials: MO
authors.lastname: Taha
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D019610:Cytoprotection; D004195:Disease Models, Animal; D004558:Electric Stimulation; D017615:Enteric Nervous System / Q000187:drug effects / Q000503:physiopathology; D005765:Gastrointestinal Agents / Q000494:pharmacology*; D005769:Gastrointestinal Motility / Q000187:drug effects; D007583:Jejunum / Q000098:blood supply* / Q000187:drug effects* / Q000294:innervation / Q000473:pathology / Q000503:physiopathology; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology
chemical_list: D005765:Gastrointestinal Agents; D011189:Potassium Chloride; D001120:Arginine