title: Hard X-ray imaging of bacterial cells: nano-diffraction and ptychographic reconstruction.
abstract: Ptychographic coherent X-ray diffractive imaging (PCDI) has been combined with nano-focus X-ray diffraction to study the structure and density distribution of unstained and unsliced bacterial cells, using a hard X-ray beam of 6.2keV photon energy, focused to about 90nm by a Fresnel zone plate lens. While PCDI provides images of the bacteria with quantitative contrast in real space with a resolution well below the beam size at the sample, spatially resolved small angle X-ray scattering using the same Fresnel zone plate (cellular nano-diffraction) provides structural information at highest resolution in reciprocal space up to 2nm(-1). We show how the real and reciprocal space approach can be used synergistically on the same sample and with the same setup. In addition, we present 3D hard X-ray imaging of unstained bacterial cells by a combination of ptychography and tomography.
authors.affiliation: Institut für Röntgenphysik, Georg-August-Universität, Göttingen, Germany. rwilke@gwdg.de
authors.forename: R N
authors.initials: RN
authors.lastname: Wilke
authors.forename: M
authors.initials: M
authors.lastname: Priebe
authors.forename: M
authors.initials: M
authors.lastname: Bartels
authors.forename: K
authors.initials: K
authors.lastname: Giewekemeyer
authors.forename: A
authors.initials: A
authors.lastname: Diaz
authors.forename: P
authors.initials: P
authors.lastname: Karvinen
authors.forename: T
authors.initials: T
authors.lastname: Salditt
mesh_terms: D017076:Computer-Aided Design; D034301:Deinococcus / Q000502:physiology* / Q000648:ultrastructure*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008859:Microscopy, Polarization / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation*; D010363:Pattern Recognition, Automated / Q000379:methods*; D014057:Tomography, X-Ray Computed / Q000295:instrumentation*