title: A comparative study of iron uptake mechanisms in marine microalgae: iron binding at the cell surface is a critical step.
abstract: We investigated iron uptake mechanisms in five marine microalgae from different ecologically important phyla: the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana, the prasinophyceae Ostreococcus tauri and Micromonas pusilla, and the coccolithophore Emiliania huxleyi. Among these species, only the two diatoms were clearly able to reduce iron, via an inducible (P. tricornutum) or constitutive (T. pseudonana) ferrireductase system displaying characteristics similar to the yeast (Saccharomyces cerevisiae) flavohemoproteins proteins. Iron uptake mechanisms probably involve very different components according to the species, but the species we studied shared common features. Regardless of the presence and/or induction of a ferrireductase system, all the species were able to take up both ferric and ferrous iron, and iron reduction was not a prerequisite for uptake. Iron uptake decreased with increasing the affinity constants of iron-ligand complexes and with increasing ligand-iron ratios. Therefore, at least one step of the iron uptake mechanism involves a thermodynamically controlled process. Another step escapes to simple thermodynamic rules and involves specific and strong binding of ferric as well as ferrous iron at the cell surface before uptake of iron. Binding was paradoxically increased in iron-rich conditions, whereas uptake per se was induced in all species only after prolonged iron deprivation. We sought cell proteins loaded with iron following iron uptake. One such protein in O. tauri may be ferritin, and in P. tricornutum, Isip1 may be involved. We conclude that the species we studied have uptake systems for both ferric and ferrous iron, both involving specific iron binding at the cell surface.
authors.affiliation: Department of Parasitology, Faculty of Science, Charles University, 12844 Prague, Czech Republic.
authors.forename: Robert
authors.initials: R
authors.lastname: Sutak
authors.forename: Hugo
authors.initials: H
authors.lastname: Botebol
authors.forename: Pierre-Louis
authors.initials: PL
authors.lastname: Blaiseau
authors.forename: Thibaut
authors.initials: T
authors.lastname: Léger
authors.forename: François-Yves
authors.initials: FY
authors.lastname: Bouget
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Camadro
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Lesuisse
mesh_terms: D059001:Aquatic Organisms / Q000254:growth & development / Q000378:metabolism*; D001345:Autoradiography; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D004579:Electron Transport / Q000187:drug effects; D038181:FMN Reductase / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000494:pharmacology; D007700:Kinetics; D008024:Ligands; D058086:Microalgae / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D008954:Models, Biological; D010084:Oxidation-Reduction / Q000187:drug effects; D010802:Phylogeny; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism
chemical_list: D007502:Iron Chelating Agents; D008024:Ligands; D007501:Iron; D038181:FMN Reductase; C023903:ferric citrate iron reductase