title: Electrochemical detection of E. coli O157:H7 using porous pseudo-carbon paste electrode modified with carboxylic multi-walled carbon nanotubes, glutaraldehyde and 3-aminopropyltriethoxysilane.
abstract: Fabrication of three different electrodes based on functional porous pseudo-carbon paste electrodes (PPCPEs) was described. PPCPEs were modified with carboxylic multi-walled carbon nanotubes (PPCPE-COOH), glutaraldehyde (PPCPE-CHO) and 3-aminopropyltriethoxysilane (PPCPE-NH2). The modified electrodes were applied in detection of E. coli O157:H7, it was showed that the electrochemical signal of PPCPE-CHO was the strongest among those three kinds of electrodes. A linear relationship between the anodic stripping peak current and the concentration of E. coli O157:H7 from 1.0 x 10(3) to 1.0 x 10(7) cells/mL and a limit of detection as low as 8.0 x 10(2) cells/mL were obtained when PPCPE-CHO was used.
authors.affiliation: Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, PR China.
authors.forename: Lijian
authors.initials: L
authors.lastname: Xu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Du
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
mesh_terms: D015374:Biosensing Techniques; D004305:Dose-Response Relationship, Drug; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D019453:Escherichia coli O157 / Q000235:genetics*; D005976:Glutaral / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D008826:Microbial Sensitivity Tests / Q000379:methods; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D016062:Porosity; D011437:Propylamines; D012821:Silanes / Q000737:chemistry*; D013997:Time Factors
chemical_list: D007074:Immunoglobulin G; D037742:Nanotubes, Carbon; D011437:Propylamines; D012821:Silanes; C477625:amino-propyl-triethoxysilane; D005976:Glutaral