title: Pathogenicity and molecular analysis of an encephalomyocarditis virus isolate from mideastern China.
abstract: Encephalomyocarditis virus (EMCV) can infect many host species and cause acute myocarditis and sudden death in preweaned piglets. In this study, an EMCV strain (NJ08) was isolated from newborn pigs with clinical signs on a pig farm in mideastern China. It was identified by indirect immunofluorescence assay and reverse-transcription polymerase chain reaction. Experiments showed that the isolate could cause severe clinical symptoms and pathological changes in mice but no obvious clinical and pathological changes in commercial piglets. Complete genomic sequencing showed that the NJ08 strain was 78.3% to 100% identical with other isolates in regions coding for various proteins. Phylogenetic analysis showed that the NJ08 isolate belonged to subgroup Ia. This study confirmed that an EMCV isolate from pigs could be fatal to mice and provided new epidemiologic data on EMCV in China.
authors.affiliation: Key Laboratory of Animal Disease Diagnosis and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Bai
authors.forename: Kangfu
authors.initials: K
authors.lastname: Jiang
authors.forename: Guolong
authors.initials: G
authors.lastname: Zhang
authors.forename: Xianwei
authors.initials: X
authors.lastname: Wang
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D001681:Biological Assay / Q000662:veterinary; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics; D018188:Cardiovirus Infections / Q000662:veterinary* / Q000821:virology; D002681:China; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
chemical_list: D036022:Capsid Proteins; D012367:RNA, Viral; C096256:VP1 protein, encephalomyocarditis virus