title: Measurement of glutathione and glutathione disulfide.
abstract: Measurements of glutathione should include quantification of both the reduced and oxidized forms. HPLC-based assays for glutathione and other cellular thiols and disulfides utilize a variety of detection methods, including ultraviolet, fluorescence, and electrochemical methods. This unit provides rapid, sensitive, highly versatile and reproducible HPLC methods for separating and quantifying sulfur-containing amino acids and related derivatives from a variety of biological samples.
authors.affiliation: Arizona Cancer Center, Tucson, Arizona, USA.
authors.forename: D
authors.initials: D
authors.lastname: Mustacich
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D055664:Electrochemical Techniques; D005978:Glutathione / Q000378:metabolism*; D019803:Glutathione Disulfide / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005978:Glutathione; D019803:Glutathione Disulfide