title: Quantitative analysis and characterization of self-assembled DNA on a silver surface.
abstract: Self-assembled monolayers of DNA on a silver surface were prepared and characterized by polarization modulation infrared reflection absorption spectroscopy (PM-IRRAS), fluorescence imaging, and (32)P radioactive labeling. The buffer concentration of the DNA solution and the surface roughness of the silver substrate were found to affect the surface coverage of DNA and its hybridization. At low buffer concentrations, surface coverage and hybridization were greatly reduced. Ethidium bromide intercalated into the adsorbed dsDNA clearly indicates the presence of dsDNA.
authors.affiliation: Department of Chemical Physics, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Karuppannan Senthil
authors.initials: KS
authors.lastname: Kumar
authors.forename: Ron
authors.initials: R
authors.lastname: Naaman
mesh_terms: D004247:DNA / Q000737:chemistry*; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013499:Surface Properties
chemical_list: D012834:Silver; D004247:DNA