title: In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants.
abstract: Interaction between cells and implant surface is crucial for clinical success. This interaction and the associated surface treatment are essential for achieving a fast osseointegration process. Several studies of different topographical or chemical surface modifications have been proposed previously in literature. The Biomimetic Advanced Surface (BAS) topography is a combination of a shot blasting and anodizing procedure. Macroroughness, microporosity of titanium oxide and Calcium/Phosphate ion deposition is obtained. Human mesenchymal stem cells (hMCSs) response in vitro to this treatment has been evaluated. The results obtained show an improved adhesion capacity and a higher proliferation rate when hMSCs are cultured on treated surfaces. This biomimetic modification of the titanium surface induces the expression of osteblastic differentiation markers (RUNX2 and Osteopontin) in the absence of any externally provided differentiation factor. As a main conclusion, our biomimetic surface modification could lead to a substantial improvement in osteoinduction in titanium alloy implants.
authors.affiliation: Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, University of Zaragoza, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Santander
authors.forename: Clara
authors.initials: C
authors.lastname: Alcaine
authors.forename: Jaber
authors.initials: J
authors.lastname: Lyahyai
authors.forename: Maria Angeles
authors.initials: MA
authors.lastname: Pérez
authors.forename: Clementina
authors.initials: C
authors.lastname: Rodellar
authors.forename: Manuel
authors.initials: M
authors.lastname: Doblaré
authors.forename: Ignacio
authors.initials: I
authors.lastname: Ochoa
mesh_terms: D000497:Alloys; D000537:Aluminum Oxide / Q000737:chemistry; D040761:Biomimetic Materials / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D048430:Cell Shape; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D003722:Dental Alloys / Q000737:chemistry*; D015921:Dental Implants; D055664:Electrochemical Techniques; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000378:metabolism / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D053495:Osteopontin / Q000032:analysis; D056951:Photoelectron Spectroscopy; D016062:Porosity; D060888:Real-Time Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D050659:Core Binding Factor Alpha 1 Subunit; D003722:Dental Alloys; D015921:Dental Implants; C493709:RUNX2 protein, human; D053495:Osteopontin; C031462:titanium alloy (TiAl6V4); C009495:titanium dioxide; C020243:calcium phosphate; D014025:Titanium; D000537:Aluminum Oxide