title: Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica released by Nonidet P40.
abstract: Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Ceredigion, Wales SY23 3FG, UK. rom@aber.ac.uk
authors.forename: Russell M
authors.initials: RM
authors.lastname: Morphew
authors.forename: Clare M
authors.initials: CM
authors.lastname: Hamilton
authors.forename: Hazel A
authors.initials: HA
authors.lastname: Wright
authors.forename: David J
authors.initials: DJ
authors.lastname: Dowling
authors.forename: Sandra M
authors.initials: SM
authors.lastname: O'Neill
authors.forename: Peter M
authors.initials: PM
authors.lastname: Brophy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005210:Fasciola hepatica / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015801:Helminth Proteins / Q000737:chemistry / Q000378:metabolism; D007155:Immunologic Factors / Q000737:chemistry* / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000737:chemistry*; D016415:Sequence Alignment
chemical_list: D015801:Helminth Proteins; D007155:Immunologic Factors; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40