title: Activation of calcineurin is mainly responsible for the calcium sensitivity of gene deletion mutations in the genome of budding yeast.
abstract: Here we have identified 120 gene deletion mutants that are sensitive to 0.4M calcium in Saccharomyces cerevisiae. Twenty-seven of these mutants are of genes involved in the vacuolar protein sorting pathway, including those encoding the seven components of the ESCRT complexes, and ten of them encode the components and assembly factors of the vacuolar H(+)-ATPase. Both Mediator and Paf1 complexes modulating the activity of the general transcription machinery are involved in the calcium sensitivity of yeast cells. Most of these mutants show elevated intracellular calcium contents in response to calcium stress. The calcium sensitivity of 106 mutants can be completely suppressed by 10mM Mg(2+), 56 of which can also be suppressed by the inhibitor of calcineurin, cyclosporine A. Therefore, the calcium sensitivity of nearly a half of these 120 mutations is at least partially due to the activation of calcineurin and can be modulated by magnesium ion.
authors.affiliation: The State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
authors.forename: Yunying
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jingcai
authors.initials: J
authors.lastname: Du
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Linghuo
authors.initials: L
authors.lastname: Jiang
mesh_terms: D019703:Calcineurin / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D016572:Cyclosporine / Q000494:pharmacology; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D017353:Gene Deletion; D016681:Genome, Fungal; D042541:Intracellular Space / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D056892:Mediator Complex / Q000235:genetics / Q000378:metabolism; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D004791:Enzyme Inhibitors; D056892:Mediator Complex; D029701:Saccharomyces cerevisiae Proteins; D016572:Cyclosporine; D019703:Calcineurin; D006180:Proton-Translocating ATPases; D008274:Magnesium; D002118:Calcium