title: [Inhibitory effect of the recombinant adenovirus pre-miR-10a on K562 cell proliferation].
abstract: OBJECTIVE
  To investigate the changes of proliferation and apoptosis in K562 cells over-expressing miR-10a.
  
  
  METHODS
  K562 cells were infected  with pAd-pre-miR-10a virus,  and then  the levels of miR-10a and bcr/abl in transfected K562 cells was detected  using RT-PCR and the level of BCR/ABL using Western blotting. The methylthiazolyl tetrazolium (MTT) assay and flow cytometry (FCM) were used to monitor the changes of proliferation and apoptosis of K562 cells after transfection.
  
  
  RESULTS
  Compared with the control groups,  K562 cells transfected with pAd-pre-miR-10a presented the significantly increased level of miR-10a,  the significantly decreased expressions of bcr/abl fusion gene and BCR/ABL fusion protein,  the inhibited proliferation ability and the promoted apoptosis (P<0.05).
  
  
  CONCLUSIONS
  The recombinant adenovirus of pAd-pre-miR-10a can inhibit the proliferation of K562 cells and promote cell apoptosis significantly.
authors.affiliation: Department of Immunology, Center for Molecular Medicine and Cancer Research, Chongqing, China.
authors.forename: Jie-yu
authors.initials: JY
authors.lastname: Qi
authors.forename: Kun
authors.initials: K
authors.lastname: Tao
authors.forename: Can-wei
authors.initials: CW
authors.lastname: Wang
authors.forename: Qiu-hong
authors.initials: QH
authors.lastname: Li
authors.forename: Yi-ping
authors.initials: YP
authors.lastname: Deng
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics; D049109:Cell Proliferation; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D020014:K562 Cells; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism
chemical_list: C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D016044:Fusion Proteins, bcr-abl