title: In vitro evaluation of the effects of different treatment procedures on dentine tubules.
abstract: OBJECTIVE
  The objective of this preliminary study was to evaluate the occluding effect of topical gaseous ozone application and Er:YAG laser on human dentine tubules by scanning electron microscopic (SEM) analysis.
  
  
  METHODS
  Twenty-four dentine specimens were divided into three groups. Group I, including specimens treated only with citric acid, served as the control. Group II was treated with Er:YAG (30 Hz 60 mJ, 10 sec), and Group III received only topical gaseous ozone application (10 sec at level 2). Diameters and the number of open dentinal tubules per 100 μm(2) were counted from SEM photomigrophraphs at ×2000 magnification.
  
  
  RESULTS
  In terms of diameters and numbers of open dentinal tubules per 100 μm(2), both treatment modalities presented significant occlusion. The mean values of the diameters and the number of open dentinal tubules per 100 μm(2) were observed in Group I, Group II, and Group III, in decreasing order. Significant differences were found among the groups in favor of the topical gaseous ozone applied group.
  
  
  CONCLUSIONS
  The dentine tubules in both treatment groups were occluded, however more marked occlusion were seen in ozone treated group.
authors.affiliation: Department of Periodontology, Yeditepe University, Istanbul, Turkey. haregursoy@hotmail.com
authors.forename: Hare
authors.initials: H
authors.lastname: Gürsoy
authors.forename: Gökser
authors.initials: G
authors.lastname: Cakar
authors.forename: Sebnem Dirikan
authors.initials: SD
authors.lastname: Ipçi
authors.forename: Bahar
authors.initials: B
authors.lastname: Kuru
authors.forename: Selçuk
authors.initials: S
authors.lastname: Yilmaz
mesh_terms: D000287:Administration, Topical; D000328:Adult; D003804:Dentin / Q000187:drug effects / Q000528:radiation effects* / Q000648:ultrastructure*; D006801:Humans; D066298:In Vitro Techniques; D053844:Lasers, Solid-State; D028022:Low-Level Light Therapy / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008963:Molar / Q000187:drug effects / Q000528:radiation effects; D010126:Ozone / Q000494:pharmacology; D012016:Reference Values; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D055815:Young Adult
chemical_list: D010126:Ozone