title: Anti-arthritic effect of eugenol on collagen-induced arthritis experimental model.
abstract: This study was designed to test the efficacy of eugenol, a compound obtained from the essential oil of cloves (Syzygium aromaticum) in collagen-induced arthritis (CIA), a well characterized murine model of rheumatoid arthritis. Macroscopic clinical evidence of CIA manifests first as periarticular erythema and edema in the hind paws. Treatment with eugenol starting at the onset of arthritis (day 25) ameliorated these clinical signs of CIA. Furthermore, eugenol inhibited mononuclear cell infiltration into the knee joints of arthritic mice and also lowered the levels of cytokines (tumor necrosis factor (TNF)-α, interferon (IFN)-γ and tumor growth factor (TGF)-β) within the ankle joints. Eugenol treatment did not affect the in vitro cell viability as assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Therefore, eugenol ameliorates experimental arthritis and could be useful as a beneficial supplement in treating human arthritis.
authors.affiliation: Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, Pr 87020-900, Brazil. r_grespan@yahoo.com.br
authors.forename: Renata
authors.initials: R
authors.lastname: Grespan
authors.forename: Marcia
authors.initials: M
authors.lastname: Paludo
authors.forename: Henrique de Paula
authors.initials: Hde P
authors.lastname: Lemos
authors.forename: Carmem Patrícia
authors.initials: CP
authors.lastname: Barbosa
authors.forename: Ciomar Aparecida
authors.initials: CA
authors.lastname: Bersani-Amado
authors.forename: Marcia Machado de Oliveira
authors.initials: MM
authors.lastname: Dalalio
authors.forename: Roberto Kenji Nakamura
authors.initials: RK
authors.lastname: Cuman
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D005054:Eugenol / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009504:Neutrophils / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D005054:Eugenol