title: Determination of limonin in dog plasma by liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study.
abstract: A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of limonin in beagle dog plasma using nimodipine as internal standard. The analyte and internal standard (IS) were extracted with ether followed by a rapid isocratic elution with 10 mm ammonium acetate buffer-methanol (26:74, v/v) on a C18 column (150 × 2.1 mm i.d.) and subsequent analysis by mass spectrometry in the multiple reaction monitoring mode. The precursor to product ion transitions of m/z 469.4 → 229.3 and m/z 417.2 → 122.0 were used to measure the analyte and the IS. The assay was linear over the concentration range of 0.625-100 ng/mL for limonin in dog plasma. The lower limit of quantification was 0.312 ng/mL and the extraction recovery was >90.4% for limonin. The inter- and intra-day precision of the method at three concentrations was less than 9.9%. The method was successfully applied to pharmacokinetic study of limonin in dogs.
authors.affiliation: Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, 155 Hanzhong Road, Nanjing, China.
authors.forename: Shi-jia
authors.initials: SJ
authors.lastname: Liu
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Bo-yang
authors.initials: BY
authors.lastname: Yu
authors.forename: Chang-yin
authors.initials: CY
authors.lastname: Li
authors.forename: Zi-xiu
authors.initials: ZX
authors.lastname: Liu
authors.forename: Wen-zheng
authors.initials: WZ
authors.lastname: Ju
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D005260:Female; D057230:Limit of Detection; D036701:Limonins / Q000097:blood*; D008297:Male; D009553:Nimodipine / Q000097:blood; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000085:Acetates; D002021:Buffers; D036701:Limonins; D009553:Nimodipine; C001546:limonin; C018824:ammonium acetate