title: Isolation and Typing of Canine Parvovirus in CRFK Cell Line in Puducherry, South India.
abstract: A total of 128 faecal samples/rectal swabs were collected from dogs showing signs of diarrhea/enteritis in and around Puducherry, South India. Eighteen clinical samples, showing high HA titre of 1:512 and above and positivity by polymerase chain reaction (PCR) with CPV-2ab primers, were subjected to virus isolation in CRFK cell line. Of the 18 samples processed, 3 samples (16.6%) were positive for CPV and were confirmed by haemagglutination, dot-ELISA, and IFAT. The three cell culture isolates were characterized as CPV-2b types by multiplex PCR as well as by monoclonal antibody typing.
authors.affiliation: Department of Veterinary Microbiology, Rajiv Gandhi College of Veterinary and Animal Sciences, Puducherry, 605009 India  ; 10/442, 1st Cross, Thulukathamman Nagar, Murungapakkam Post, Pondicherry, 605004 India.
authors.forename: S
authors.initials: S
authors.lastname: Parthiban
authors.forename: Hirak Kumar
authors.initials: HK
authors.lastname: Mukhopadhyay
authors.forename: D
authors.initials: D
authors.lastname: Panneer
authors.forename: P X
authors.initials: PX
authors.lastname: Antony
authors.forename: R M
authors.initials: RM
authors.lastname: Pillai