title: Intravital microscopy to image membrane trafficking in live rats.
abstract: Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. Here, we describe a series of procedures designed to image subcellular structures, such as endosomes and secretory vesicles in the salivary glands (SGs) of live rats. To this aim, we used fluorescently labeled molecules and/or fluorescently tagged proteins that were transiently transfected in the live animal.
authors.affiliation: Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, USA.
authors.forename: Andrius
authors.initials: A
authors.lastname: Masedunskas
authors.forename: Monika
authors.initials: M
authors.lastname: Sramkova
authors.forename: Laura
authors.initials: L
authors.lastname: Parente
authors.forename: Roberto
authors.initials: R
authors.lastname: Weigert
mesh_terms: D000818:Animals; D004705:Endocytosis; D005089:Exocytosis; D005456:Fluorescent Dyes / Q000737:chemistry; D018014:Gene Transfer Techniques; D018613:Microscopy, Confocal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical / Q000295:instrumentation; D013194:Staining and Labeling; D013363:Submandibular Gland / Q000166:cytology*; D059008:Time-Lapse Imaging
chemical_list: D005456:Fluorescent Dyes