title: Characterization of rotavirus causing acute diarrhoea in children in Kathmandu, Nepal, showing the dominance of serotype G12.
abstract: Diarrhoeal diseases are a major problem in developing countries. Though precise data on childhood mortality associated with diarrhoeal diseases in Nepal are not available, it has been estimated that approximately 25 % of child deaths are associated with diarrhoeal disease, particularly acute diarrhoea. The purpose of this study was to assess the incidence of rotavirus causing acute diarrhoea in children less than 5 years of age. A total of 525 children with acute diarrhoea in a children's hospital of Kathmandu, Nepal, were enrolled between April and September 2011. The incidence of acute diarrhoea due to rotavirus was 25.9 % (136/525) as determined by ELISA. The percentage of rotavirus-infected males was higher (64.5 %) than females (35.5 %). The frequency of rotavirus cases was higher in children less than 2 years of age, among which the majority of cases (80.2 %) were in children between 6 and 24 months old (P<0.01). Genotypic characterization by RT-PCR revealed that the serotype G12 represented 55.9 % of cases in this study associated with P-types of either P[6], P[4] or P[8]. Further to this, a total of eight G/P combinations were identified, G12P[6] being the most common strain type of rotavirus in Nepal, with a prevalence rate of 46.4 %. The aim of this study was to find out the major genotypes of rotavirus causing acute diarrhoea in children.
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Shamshul
authors.initials: S
authors.lastname: Ansari
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Jeevan Bahadur
authors.initials: JB
authors.lastname: Sherchand
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Basista Prasad
authors.initials: BP
authors.lastname: Rijal
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Keshab
authors.initials: K
authors.lastname: Parajuli
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Shyam Kumar
authors.initials: SK
authors.lastname: Mishra
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Rajan Kumar
authors.initials: RK
authors.lastname: Dahal
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Shovita
authors.initials: S
authors.lastname: Shrestha
authors.affiliation: Public Health Research Laboratory, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Sarmila
authors.initials: S
authors.lastname: Tandukar
authors.affiliation: Nepalese Army Institute of Health Sciences, Kathmandu, Nepal.
authors.forename: Raina
authors.initials: R
authors.lastname: Chaudhary
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Hari Prasad
authors.initials: HP
authors.lastname: Kattel
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Amul
authors.initials: A
authors.lastname: Basnet
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Bharat Mani
authors.initials: BM
authors.lastname: Pokhrel
mesh_terms: D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology*; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D009390:Nepal / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012401:Rotavirus / Q000145:classification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*; D012703:Serotyping