title: Titers of ABO antibodies in group O blood donors.
abstract: BACKGROUND
  Plasma components of group O blood donations are rarely submitted to ABO antibody titrations even though it is well known that passively acquired antibodies may destroy the recipient's own red cells and tissue grafts.
  
  
  OBJECTIVE
  Thus, group O donations stratified by gender and age were randomly titrated to identify the best source of products for apheresis and exsanguinous transfusion.
  
  
  METHODS
  Samples from 603 blood donors were tested by ABO antibody titration using the conventional tube technique at room temperature. ABO antibody levels higher than 64 were considered high. After correction for gender, statistical analyses were performed using the Fisher exact and Kruskal-Wallis tests.
  
  
  RESULTS
  Most donors in the blood bank were male (65.7%). ABO antibody titers ranged from 1 to 2048. The estimations of prevalence for the titers were: anti-A,B < 128 = 86.9% and = 128 = 2.16%; Anti-A = 128 = 9.29% and anti-B = 128 = 4.81%. Low mean titers for both anti-A and anti-B antibodies were found in over 50-year-old men (p-value = 0.040). High anti-B antibody levels were found in young women (p-value = 0.002).
  
  
  CONCLUSIONS
  This study confirms that over 50-year-old O group men should be selected as blood donors in non-identical ABO transfusion situations. Also, titration of ABO antibodies in blood banks will increase safety in non-identical ABO transfusions.
authors.affiliation: Banco de Sangue de São Paulo, São Paulo, SP, Brazil.
authors.forename: Natalia Dallaval Galvão
authors.initials: ND
authors.lastname: de França
authors.forename: Mônica Caamaño Cristovão
authors.initials: MC
authors.lastname: Poli
authors.forename: Patrícia Guilhem de Almeida
authors.initials: PG
authors.lastname: Ramos
authors.forename: Cláudia Strang da Rocha
authors.initials: CS
authors.lastname: Borsoi
authors.forename: Rafael
authors.initials: R
authors.lastname: Colella