title: Phospholipase D participates in H(2)O(2)-induced A549 alveolar epithelial cell migration.
abstract: To investigate the effects of phospholipase D (PLD) on low-concentration hydrogen peroxide (H(2)O(2))-induced growth and migration in alveolar epithelial A549 cells, the cells were exposed to H(2)O(2) (3-100 μM) for 12-48 hours, cell proliferation was determined by MTT assay and cell migration was tested by a modified epithelial wound healing assay. We found that one bolus of H(2)O(2) (10-100 μM) did not affect proliferation, but significantly stimulated migration (143-161% of control) after a 12-hour exposure. Pretreatment with the antioxidants catalase (1000 U/ml), N-acetyl-cysteine (2 mM), or edaravone (10 μM) abolished the migration induced by 30 μM H(2)O(2); the PLD inhibitor 1-butanol (0.5%) also attenuated H(2)O(2)-induced migration to the control level; while exogenous phosphatidic acid (PA) (10(-7)-10(-4) M) mimicked the effects of PLD activation and induced migration in a dose-dependent manner. We suggest that the alveolar epithelial cell migration induced by exposure to low concentrations of H(2)O(2) benefits tissue repair during acute lung injury (ALI) and PLD is involved in the underlying mechanism.
authors.affiliation: Department of Cardiothoracic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: Zhe-Ren
authors.initials: ZR
authors.lastname: Shao
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Xu
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yun-Bi
authors.initials: YB
authors.lastname: Lu
authors.forename: Gang
authors.initials: G
authors.lastname: Shen
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
mesh_terms: D020001:1-Butanol / Q000494:pharmacology; D056809:Alveolar Epithelial Cells / Q000187:drug effects* / Q000201:enzymology; D000975:Antioxidants / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004336:Drug Antagonism; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000201:enzymology; D016877:Oxidants / Q000037:antagonists & inhibitors / Q000633:toxicity*; D010712:Phosphatidic Acids / Q000494:pharmacology; D010739:Phospholipase D / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004396:Coloring Agents; D016877:Oxidants; D010712:Phosphatidic Acids; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; D020001:1-Butanol; D006861:Hydrogen Peroxide; D010739:Phospholipase D; C022616:thiazolyl blue