title: Fast synchronized dual-wavelength laser speckle imaging system for monitoring hemodynamic changes in a stroke mouse model.
abstract: In this Letter, we describe a newly developed synchronized dual-wavelength laser speckle contrast imaging system, which contains two cameras that are synchronously triggered to acquire data. The system can acquire data at a high spatiotemporal resolution (up to 500 Hz for ~1000×1000 pixels). A mouse model of stroke is used to demonstrate the capability for imaging the fast changes (within tens of milliseconds) in oxygenated and deoxygenated hemoglobin concentration, and the relative changes in blood flow in the mouse brain, through an intact cranium. This novel imaging technology will enable the study of fast hemodynamics and metabolic changes in vascular diseases.
authors.affiliation: Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Qin
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.forename: Suzan
authors.initials: S
authors.lastname: Dziennis
authors.forename: Roberto
authors.initials: R
authors.lastname: Reif
authors.forename: Ruikang K
authors.initials: RK
authors.lastname: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000378:metabolism; D004195:Disease Models, Animal; D006439:Hemodynamics; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism / Q000503:physiopathology*; D007834:Lasers; D008670:Metals / Q000737:chemistry; D051379:Mice; D057054:Molecular Imaging / Q000295:instrumentation*; D010087:Oxides / Q000737:chemistry; D010100:Oxygen / Q000378:metabolism; D012542:Scattering, Radiation; D012666:Semiconductors
chemical_list: D008670:Metals; D010087:Oxides; D010100:Oxygen