title: Effects of autologous, cross-linked erythrocytes on isolated hypoperfused rabbit heart dynamics.
abstract: The present study was aimed at assessing the effects of either red blood cells (RBC) or RBC cross-linked with the bifunctional dimethyl suberimidate reagent (C-RBC) on contractile force (CFo), heart rate (HR) and coronary flow (CF) of the isolated rabbit heart hypoperfused with RBC suspensions under 30 mm Hg constant pressure. RBC or C-RBC caused a rapid and marked reduction of CF, CFo and HR. In RBC-treated hearts, however, reperfusion with Tyrode solution partially restored the initial myocardial parameters, while in C-RBC-treated hearts a rapid impairment of diastolic relaxation with a subsequent, steady and increasing heart contracture was observed. Histological analysis showed that in C-RBC-perfused hearts either capillaries or precapillary arterioles were occluded by C-RBC in spite of extensive washings with Tyrode solution. These findings indicate that C-RBC impair coronary circulation markedly and irreversibly.
authors.affiliation: Dipartimento di Neuroscienze, Sezione di Farmacologia, Università degli Studi di Siena, Siena, Italy. frosinim@unisi.it
authors.forename: Maria
authors.initials: M
authors.lastname: Frosini
authors.forename: Alessandra
authors.initials: A
authors.lastname: Larini
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Ricci
authors.forename: Luisa
authors.initials: L
authors.lastname: Lucas
authors.forename: Beatrice
authors.initials: B
authors.lastname: Gorelli
authors.forename: Giampietro
authors.initials: G
authors.lastname: Sgaragli
authors.forename: Piero
authors.initials: P
authors.lastname: Tanganelli
authors.forename: Massimo
authors.initials: M
authors.lastname: Valoti
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004120:Dimethyl Suberimidate / Q000494:pharmacology*; D004912:Erythrocytes; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D006339:Heart Rate / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D010477:Perfusion; D011817:Rabbits
chemical_list: D003432:Cross-Linking Reagents; D004120:Dimethyl Suberimidate; D000255:Adenosine Triphosphate