title: Ecological roles and release patterns of acylated homoserine lactones in Pseudomonas sp. HF-1 and their implications in bacterial bioaugmentation.
abstract: To enable development of a better bacterial bioaugmentation system for tobacco wastewater treatment, the roles and release patterns of acylated homoserine lactones (AHLs) in Pseudomonas sp. HF-1 were evaluated. Swarming was found to be induced by N-hexanoyl-homoserine lactone (C(6)-HSL) and N-3-oxo-hexanoyl-homoserine lactone (3-oxo-C(6)-HSL); the formation of extracellular polymeric substances (EPS) was induced by 3-oxo-C(6)-HSL, C(6)-HSL and N-3-oxo-octanoyl-homoserine lactone (3-oxo-C(8)-HSL); and biofilm formation was induced by C(6)-HSL and 3-oxo-C(8)-HSL. When the culture conditions were 25°C, pH 5-6, 3% inoculum, 1.5 g L(-1) nicotine and 1% NaCl, the amount of AHLs released was sufficient for quorum sensing of swarming and EPS formation for strain HF-1, which was beneficial to the startup stage during bioaugmentation. When strain HF-1 was cultured at pH 8 in the presence of 1.2-1.8 g L(-1) of nicotine and 1% NaCl, the threshold for quorum sensing of biofilm formation was reached and the bioaugmentation system showed an efficient performance.
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
authors.forename: Mei-zhen
authors.initials: MZ
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.forename: Hong-zhen
authors.initials: HZ
authors.lastname: He
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Shen
authors.forename: Hua-jun
authors.initials: HJ
authors.lastname: Feng
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D049108:Cell Enlargement; D004463:Ecology; D009538:Nicotine / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification* / Q000378:metabolism*; D053038:Quorum Sensing / Q000502:physiology*; D013045:Species Specificity
chemical_list: D054742:Acyl-Butyrolactones; D009538:Nicotine