title: Genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin.
abstract: Streptomyces davawensis JCM 4913 synthesizes the antibiotic roseoflavin, a structural riboflavin (vitamin B(2)) analog. Here, we report the 9,466,619-bp linear chromosome of S. davawensis JCM 4913 and a 89,331-bp linear plasmid. The sequence has an average G+C content of 70.58% and contains six rRNA operons (16S-23S-5S) and 69 tRNA genes. The 8,616 predicted protein-coding sequences include 32 clusters coding for secondary metabolites, several of which are unique to S. davawensis. The chromosome contains long terminal inverted repeats of 33,255 bp each and atypical telomeres. Sequence analysis with regard to riboflavin biosynthesis revealed three different patterns of gene organization in Streptomyces species. Heterologous expression of a set of genes present on a subgenomic fragment of S. davawensis resulted in the production of roseoflavin by the host Streptomyces coelicolor M1152. Phylogenetic analysis revealed that S. davawensis is a close relative of Streptomyces cinnabarinus, and much to our surprise, we found that the latter bacterium is a roseoflavin producer as well.
authors.affiliation: Department of Biotechnology, Institute for Technical Microbiology, Mannheim University of Applied Sciences, Mannheim, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Jankowitsch
authors.forename: Julia
authors.initials: J
authors.lastname: Schwarz
authors.forename: Christian
authors.initials: C
authors.lastname: Rückert
authors.forename: Bertolt
authors.initials: B
authors.lastname: Gust
authors.forename: Rafael
authors.initials: R
authors.lastname: Szczepanowski
authors.forename: Jochen
authors.initials: J
authors.lastname: Blom
authors.forename: Stefan
authors.initials: S
authors.lastname: Pelzer
authors.forename: Jörn
authors.initials: J
authors.lastname: Kalinowski
authors.forename: Matthias
authors.initials: M
authors.lastname: Mack
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001483:Base Sequence; D016680:Genome, Bacterial; D005810:Multigene Family; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D012256:Riboflavin / Q000031:analogs & derivatives / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000145:classification / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; C009191:roseoflavin; D012256:Riboflavin