title: Measurement of cGMP and soluble guanylyl cyclase activity.
abstract: Soluble guanylyl cyclase is responsible for synthesis of guanosine 3'5'-cyclic monophosphate. The enzyme is activated by NO, so measurement of tissue levels of cGMP and the activity of guanylyl cyclase is important in studies of NO-cGMP signal transduction. The method of choice for measuring tissue levels of cGMP is a radioimmunoassay, but it can also be measured using a scintillation proximity assay. Guanylyl cyclase activity is determined by measuring the conversion of GTP to cGMP using a radioimmunoassay or by following the conversion of [³²P]GTP to [³²P]cGMP, with separation of the product by precipitation and chromatography on an alumina column.
authors.affiliation: Glaxo Wellcome Medicines Research Center, Stevenage, United Kingdom.
authors.forename: E
authors.initials: E
authors.lastname: Southam
mesh_terms: D000818:Animals; D006152:Cyclic GMP / Q000032:analysis* / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism*; D006801:Humans; D010761:Phosphorus Radioisotopes; D011863:Radioimmunoassay; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D000071756:Soluble Guanylyl Cyclase
chemical_list: D010761:Phosphorus Radioisotopes; D018160:Receptors, Cytoplasmic and Nuclear; D006160:Guanosine Triphosphate; D006162:Guanylate Cyclase; D000071756:Soluble Guanylyl Cyclase; D006152:Cyclic GMP