title: Characterization of physiological response and identification of associated genes under heat stress in rice seedlings.
abstract: Global warming, which is caused by greenhouse gas emissions, makes food crops more vulnerable to heat stress. Understanding the heat stress-related mechanisms in crops and classifying heat stress-related genes can increase our knowledge in heat-resistant molecular biology and propel developments in molecular design breeding, which can help rice cope with unfavorable temperatures. In this study, we carried out a physiological analysis of rice plants after heat stress. The results show a dramatic increase in malondialdehyde contents and SOD activities. We successfully isolated 11 heat-related rice genes with known function annotation through DNSH, which is an improved SSH method for screening long cDNA fragments. The reanalysis of microarray data from public database revealed that all these genes displayed various expression patterns after heat stress, drought, cold and salt. Quantitative real-time reverse transcription PCR was also performed to validate the expression of these genes after heat stress. The expressions in 10 genes were all significantly changed except for contig 77, which is a CBL-interacting protein kinase. Several reports have been published about the members of the same gene family.
authors.affiliation: College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Xue
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Long-Biao
authors.initials: LB
authors.lastname: Guo
authors.forename: Da-Li
authors.initials: DL
authors.lastname: Zeng
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Dong
authors.forename: Guo-Chang
authors.initials: GC
authors.lastname: Sun
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D003080:Cold Temperature; D055864:Droughts; D015870:Gene Expression; D017343:Genes, Plant; D057232:Global Warming; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006358:Hot Temperature; D008315:Malondialdehyde / Q000378:metabolism; D046228:Microarray Analysis / Q000379:methods; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D018384:Oxidative Stress / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D055049:Salt Tolerance; D036226:Seedlings / Q000235:genetics / Q000502:physiology; D012965:Sodium Chloride; D013312:Stress, Physiological / Q000235:genetics*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012965:Sodium Chloride; D008315:Malondialdehyde; D013482:Superoxide Dismutase