title: TGF-β suppresses the expression of genes related to mitochondrial function in lung A549 cells.
abstract: TGF-β is a mediator of lung fibrosis and regulates the alveolar epithelial type II cell phenotype. TGF-β can induce epithelial mesenchymal transition of idiopathic pulmonary disease and cancer metastasis. Peroxisome proliferator-activated receptor gamma co-activator 1-alpha (PGC-1 α) is a key metabolic regulator that stimulates mitochondrial biogenesis and promotes remodeling of muscle tissue to oxidative fiber-type composition. Here, we report that the induction of TGF-β decreased mRNA expression of PGC-1α, and PGC-1 target genes, such as the transcription factors NRF-2, ERR-α, and PPAR-γ in lung epithelial A549 cells. In addition, TGF-β led to the reduction of super oxide dismutase 2 (anti-oxidant enzyme), cytochrome C (electron transport chain in mitochondria), and MCAD (a mitochondrial β-oxidant enzyme) in A549 cells. Together, our results suggest that TGF-β may suppress the transcriptional activity of the genes related to mitochondrial biogenesis or function. This mechanism may provide a novel insight into the understanding of fibrosis disease.
authors.affiliation: Asbestos Environmental Health Center, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University, Yangsan Hospital, Yangsan, Korea. eunjungs93@gmail.com
authors.forename: E J
authors.initials: EJ
authors.lastname: Sohn
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Hwang
authors.forename: S
authors.initials: S
authors.lastname: Im
authors.forename: Y
authors.initials: Y
authors.lastname: Moon
authors.forename: D M
authors.initials: DM
authors.lastname: Kang
mesh_terms: D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D000097983:ERRalpha Estrogen-Related Receptor; D000099156:Superoxide Dismutase 2
chemical_list: D045304:Cytochromes c; D006360:Heat-Shock Proteins; D051267:NF-E2-Related Factor 2; D047495:PPAR gamma; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D011960:Receptors, Estrogen; D013482:Superoxide Dismutase; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D000097983:ERRalpha Estrogen-Related Receptor; D000099156:Superoxide Dismutase 2; C495635:NFE2L2 protein, human; C486497:PPARGC1A protein, human