title: [The protective effects of inhibition of c-Jun N-terminal kinase signal pathway on the intestinal barrier in rats with endotoxemia].
abstract: OBJECTIVE
  To examine the protective effects of inhibition of c-jun N-terminal kinase (JNK) stress signal pathway on the injured barrier in endotoxemic rats.
  
  
  METHODS
  Twenty-four male Sprague Dwaley (SD) rats were randomly divided into control group, endotoxemia model group and JNK inhibitor group (n=8 each) to receive administration of: 1 normal saline 2 ml/kg + PPCES 2.5 ml/kg [vehicle of JNK inhibitor (SP600125), control group]; 2 lipopolysaccharide (LPS) 10 mg/kg + PPCES 2.5 ml/kg (endotoxemia model group); 3 LPS 10 mg/kg + JNK inhibitor (SP600125) 10 mg/kg (JNK inhibitor group). The activity and survival rate of the rats were recorded. Ileum tissue samples were collected 12 hours after drug administration for pathological examination. Blood samples were collected at the same time for determination of concentration of D-lactate by enzyme linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Rats in control group were active normally, and there was no death. Pathological examination showed there was edema of ileal mucosa, and shortening of villus and inflammatory cell infiltration in model group as compared with control group. JNK inhibitor greatly ameliorated the lesions compared with model group. The concentration of D-lactate (µg/L) in model group was significantly higher than that in control group. (943.8 ± 439.6 vs 227.9 ± 130.0, P<.05). JNK inhibitor could decrease the plasma D-lactate concentration (637.4 ± 114.4 vs 943.8 ± 439.6, P<.05).
  
  
  CONCLUSIONS
  Inhibition of the JNK stress signal pathway could attenuate the intestinal barrier injury in endotoxemic rats.
authors.affiliation: Department of Critical Care Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong, China.
authors.forename: Chuan-xi
authors.initials: CX
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Ouyang
authors.forename: Jian-bo
authors.initials: JB
authors.lastname: Lai
authors.forename: Chun-fang
authors.initials: CF
authors.lastname: Qiu
authors.forename: Xiang-dong
authors.initials: XD
authors.lastname: Guan
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D019446:Endotoxemia / Q000378:metabolism* / Q000503:physiopathology; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D007422:Intestines / Q000473:pathology; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D019344:Lactic Acid / Q000097:blood; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D000873:Anthracenes; C432165:pyrazolanthrone; D019344:Lactic Acid; D048031:JNK Mitogen-Activated Protein Kinases