title: Amino acid analysis using core-shell particle column.
abstract: In this study, the separation efficiency of a core-shell particle column was compared with particle-packed and monolithic silica columns, which showed that the core-shell particle column had a smaller theoretical plate height and that its separation efficiency was not affected significantly by the increase in flow rate. A fast HPLC method using a core-shell particle column was developed for the determination of amino acids. 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was used as a fluorescence derivatization reagent for amino acids, followed by separation on a core-shell Kinetex C18 column. The analysis time for 21 NBD-amino acids was within 7min, which was faster than that in our previous studies with conventional particle-packed columns or monolithic silica columns. The linearities of the calibration curves for all the amino acids were found to be good over a range of injection amounts from 40fmol to 40pmol. The accuracies for the amino acid determinations were 90.9-107%. The method was proved to have potential for the fast determination of amino acids in biological samples.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Song
authors.forename: Takashi
authors.initials: T
authors.lastname: Funatsu
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsunoda
mesh_terms: D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives / Q000737:chemistry; D000596:Amino Acids / Q000097:blood* / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D016014:Linear Models; D051379:Mice; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000596:Amino Acids; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; D012822:Silicon Dioxide; D009327:4-Chloro-7-nitrobenzofurazan