title: Elaboration of a probiotic oblea from whey fermented using Lactobacillus acidophilus or Bifidobacterium infantis.
abstract: A novel probiotic product was developed, which was formulated as an oblea (wafer-type dehydrated traditional Mexican dessert) using goat sweet whey fermented with Bifidobacterium infantis or Lactobacillus acidophilus. To obtain the probiotic oblea, the fermented whey was formulated with prebiotic carbohydrates (inulin and resistant starch) and gelatin, and the preparation was poured onto a polytetrafluoroethylene-coated nonstick baking pan, dried in a convection oven, and finally dehydrated at a low relative humidity and room temperature (23±2°C). The amounts of prebiotic carbohydrates and gelatin to be used in the formulation were determined by a factorial experimental design. An untrained sensory panel evaluated 3 quality characteristics (film formation, homogeneity, and smoothness) in the final product. Three different drying temperatures were tested, namely, 40, 55, and 70°C. Bacterial survival at each temperature was determined by viable plate-counting. The best formulation, based on the quality characteristics tested, consisted of 58.33% (vol/vol) of fermented whey, 8.33% (vol/vol) of 6% (wt/vol) resistant starch dispersion, 16.66% (vol/vol) of 15% (wt/vol) inulin solution, and 16.66% (vol/vol) of a 10% (wt/vol) gelatin solution. Drying at 55±2°C for 2.66±0.22 h allowed for concentrations of probiotic bacteria above 9 log(10) cfu/g, which is above the minimum concentration required in a probiotic product.
authors.affiliation: Tecnológico de Monterrey, Campus Monterrey, Centro de Biotecnología FEMSA, Escuela de Biotecnología y Alimentos, Monterrey, Nuevo León, México.
authors.forename: G
authors.initials: G
authors.lastname: Trujillo-de Santiago
authors.forename: C P
authors.initials: CP
authors.lastname: Sáenz-Collins
authors.forename: C
authors.initials: C
authors.lastname: Rojas-de Gante
mesh_terms: D058491:Bacterial Load; D001644:Bifidobacterium / Q000378:metabolism*; D003611:Dairy Products / Q000382:microbiology* / Q000592:standards; D005285:Fermentation; D005516:Food Microbiology / Q000379:methods; D005524:Food Technology / Q000379:methods; D005780:Gelatin / Q000378:metabolism; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D008894:Milk Proteins / Q000378:metabolism*; D019936:Probiotics / Q000378:metabolism*; D013696:Temperature; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D000067816:Whey Proteins; D005780:Gelatin