title: PACSIN1, a Tau-interacting protein, regulates axonal elongation and branching by facilitating microtubule instability.
abstract: Tau is a major member of the neuronal microtubule-associated proteins. It promotes tubulin assembly and stabilizes axonal microtubules. Previous studies have demonstrated that Tau forms cross-bridges between microtubules, with some particles located on cross-bridges, suggesting that some proteins interact with Tau and might be involved in regulating Tau-related microtubule dynamics. This study reports that PACSIN1 interacts with Tau in axon. PACSIN1 blockade results in impaired axonal elongation and a higher number of primary axonal branches in mouse dorsal root ganglia neurons, which is induced by increasing the binding ability of Tau to microtubules. In PACSIN1-blocked dorsal root ganglia neurons, a greater amount of Tau is inclined to accumulate in the central domain of growth cones, and it promotes the stability of the microtubule network. Taken together, these results suggest that PACSIN1 is an important Tau binding partner in regulating microtubule dynamics and forming axonal plasticity.
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100191, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Liu
authors.forename: Kaosheng
authors.initials: K
authors.lastname: Lv
authors.forename: Zenglong
authors.initials: Z
authors.lastname: Li
authors.forename: Albert C H
authors.initials: AC
authors.lastname: Yu
authors.forename: Jianguo
authors.initials: J
authors.lastname: Chen
authors.forename: Junlin
authors.initials: J
authors.lastname: Teng
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D020439:Growth Cones / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008870:Microtubules / Q000235:genetics / Q000378:metabolism*; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D016875:tau Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; C114726:Pacsin1 protein, mouse; D010750:Phosphoproteins; D016875:tau Proteins