title: Study of carbohydrate-protein interactions using glyco-QDs with different fluorescence emission wavelengths.
abstract: QDs with different fluorescence emission wavelengths were coated with galactose, glucose, and lactose respectively. The formulas of glyco-QDs were determined by NMR and ICP-OES, and the interactions between glyco-QDs and PNA lectin were investigated by SPR. The results showed that multivalent presentation achieved by using QDs as the scaffold is an effective way to enhance the carbohydrate-protein interactions. The K(D) for the interaction of PNA with multivalent glyco-QDs is over 3 × 10(6)-fold lower than those with the same free sugars. The specific recognition for the sugar coated on the QDs by lectin is maintained. These sugar-coated QDs could be used as a fluorescent probe to label and identify glycoproteins.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing 100191, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Xi-Kai
authors.initials: XK
authors.lastname: Cui
authors.forename: Ming
authors.initials: M
authors.lastname: Zhong
authors.forename: Zhong-Jun
authors.initials: ZJ
authors.lastname: Li
mesh_terms: D002236:Carbohydrate Conformation; D005453:Fluorescence; D005690:Galactose / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D045663:Quantum Dots
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D005947:Glucose; D005690:Galactose