title: Genotyping of Giardia lamblia isolates from human in southern Iran.
abstract: Giardia lamblia cysts isolated from human faeces in South of Iran were analyzed   with PCR-restriction fragment length polymorphism (RFLP) assay, based on the detection of   glutamate dehydrogenase (gdh) genes. Among 205 faecal samples from microscopically   diagnosed giardiasis patients, the gdh gene was amplified from 172 cases with a semi nested   PCR assay and typed by RFLP analysis. Of the 172 positive samples, 128 (74.41%) were typed   as assemblage AII, 30 (17.44%) assemblage BIII, 6 (3.49%) assemblage BIV and in 8 (4.66%)   isolates, mixed assemblages AII and BIV were detected. Clinical features were available for   52 successfully typed cases and the possible correlation of Giardia assemblages and clinical   symptoms was evaluated. Both assemblages caused similar illness, but assemblage AII was   significantly more frequently associated with abdominal pain, nausea and vomiting. Since   these isolates, A and B, are of human origin, anthroponotic transmission of Giardia can be   suggested for the route of infection in this region.
authors.affiliation: Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. sarkarib@sums.ac.ir
authors.forename: B
authors.initials: B
authors.lastname: Sarkari
authors.forename: A
authors.initials: A
authors.lastname: Ashrafmansori
authors.forename: G R
authors.initials: GR
authors.lastname: Hatam
authors.forename: M H
authors.initials: MH
authors.lastname: Motazedian
authors.forename: Q
authors.initials: Q
authors.lastname: Asgari
authors.forename: I
authors.initials: I
authors.lastname: Mohammadpour
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005260:Female; D005838:Genotype; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005873:Giardiasis / Q000469:parasitology* / Q000635:transmission; D005969:Glutamate Dehydrogenase / Q000235:genetics; D006801:Humans; D007223:Infant; D007492:Iran; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics; D055815:Young Adult
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D005969:Glutamate Dehydrogenase