title: Scaling proprioceptor gene transcription by retrograde NT3 signaling.
abstract: Cell-type specific intrinsic programs instruct neuronal subpopulations before target-derived factors influence later neuronal maturation. Retrograde neurotrophin signaling controls neuronal survival and maturation of dorsal root ganglion (DRG) sensory neurons, but how these potent signaling pathways intersect with transcriptional programs established at earlier developmental stages remains poorly understood. Here we determine the consequences of genetic alternation of NT3 signaling on genome-wide transcription programs in proprioceptors, an important sensory neuron subpopulation involved in motor reflex behavior. We find that the expression of many proprioceptor-enriched genes is dramatically altered by genetic NT3 elimination, independent of survival-related activities. Combinatorial analysis of gene expression profiles with proprioceptors isolated from mice expressing surplus muscular NT3 identifies an anticorrelated gene set with transcriptional levels scaled in opposite directions. Voluntary running experiments in adult mice further demonstrate the maintenance of transcriptional adjustability of genes expressed by DRG neurons, pointing to life-long gene expression plasticity in sensory neurons.
authors.affiliation: Biozentrum, Department of Cell Biology, University of Basel, Basel, Switzerland.
authors.forename: Jun
authors.initials: J
authors.lastname: Lee
authors.forename: Andreas
authors.initials: A
authors.lastname: Friese
authors.forename: Monika
authors.initials: M
authors.lastname: Mielich
authors.forename: Markus
authors.initials: M
authors.lastname: Sigrist
authors.forename: Silvia
authors.initials: S
authors.lastname: Arber
mesh_terms: D000818:Animals; D002469:Cell Separation; D016000:Cluster Analysis; D005727:Ganglia, Spinal / Q000378:metabolism; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000378:metabolism; D020933:Neurotrophin 3 / Q000235:genetics* / Q000378:metabolism*; D005082:Physical Exertion / Q000235:genetics; D011963:Receptors, GABA-A / Q000235:genetics; D011984:Sensory Receptor Cells / Q000166:cytology / Q000378:metabolism*; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D020933:Neurotrophin 3; D011963:Receptors, GABA-A