title: Ethanolic extract of roots from Arctium lappa L. accelerates the healing of acetic acid-induced gastric ulcer in rats: Involvement of the antioxidant system.
abstract: We evaluate the curative efficacy of the ethanolic extract (EET) of roots from Arctium lappa (bardana) in healing of chronic gastric ulcers induced by 80% acetic acid in rats and additionally studies the possible mechanisms underlying this action. Oral administration of EET (1, 3, 10 and 30mg/kg) reduced the gastric lesion area in 29.2%, 41.4%, 59.3% and 38.5%, respectively, and at 10mg/kg promoted significant regeneration of the gastric mucosa, which was confirmed by proliferating cell nuclear antigen immunohistochemistry. EET (10mg/kg) treatment did not increase the gastric mucus content but restored the superoxide dismutase activity, prevented the reduction of glutathione levels, reduced lipid hydroperoxides levels, inhibited the myeloperoxidase activity and reduced the microvascular permeability. In addition, EET reduced the free radical generation and increased scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals in vitro. Furthermore, intraduodenal EET (10 and 30mg/kg) decreased volume and acidity of gastric secretion. Total phenolic compounds were high in EET (Folin-Ciocalteau assay) and the analysis by liquid chromatography-mass spectrometry revealed that the main compounds present in EET were a serie of hydroxycinnamoylquinic acid isomers. In conclusion, these data reveal that EET promotes regeneration of damaged gastric mucosa, probably through its antisecretory and antioxidative mechanisms.
authors.affiliation: Department of Pharmacology, Federal University of Parana, Curitiba, PR, Brazil.
authors.forename: Luisa Mota
authors.initials: LM
authors.lastname: da Silva
authors.forename: Alexandra
authors.initials: A
authors.lastname: Allemand
authors.forename: Daniel Augusto G B
authors.initials: DA
authors.lastname: Mendes
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Dos Santos
authors.forename: Eunice
authors.initials: E
authors.lastname: André
authors.forename: Lauro Mera
authors.initials: LM
authors.lastname: de Souza
authors.forename: Thales Ricardo
authors.initials: TR
authors.lastname: Cipriani
authors.forename: Nessana
authors.initials: N
authors.lastname: Dartora
authors.forename: Maria Consuelo Andrade
authors.initials: MC
authors.lastname: Marques
authors.forename: Cristiane Hatsuko
authors.initials: CH
authors.lastname: Baggio
authors.forename: Maria Fernanda
authors.initials: MF
authors.lastname: Werner
mesh_terms: D019342:Acetic Acid / Q000633:toxicity; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology; D031170:Arctium / Q000737:chemistry*; D002199:Capillary Permeability / Q000187:drug effects; D000431:Ethanol / Q000737:chemistry; D005260:Female; D005609:Free Radicals / Q000378:metabolism; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D005609:Free Radicals; D010636:Phenols; D010936:Plant Extracts; D000431:Ethanol; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D019342:Acetic Acid