title: Immunocytochemical analysis of cyclic nucleotides.
abstract: Visualization of second messenger molecules is a powerful approach to study the role of second messengers in relation to the (sub)cellular localization and/or kinetics of the second messenger response. This unit describes the appropriate fixation procedures for the cyclic nucleotides cAMP and cGMP and the use of specific antibodies to localize these molecules.
authors.affiliation: Maastricht University, Maastricht, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: de Vente
authors.forename: H W
authors.initials: HW
authors.lastname: Steinbusch
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001921:Brain / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007106:Immune Sera / Q000032:analysis / Q000302:isolation & purification; D007150:Immunohistochemistry; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D016707:Tissue Fixation
chemical_list: D007106:Immune Sera; D009712:Nucleotides, Cyclic