title: A multiplex RT-PCR for rapid and simultaneous detection of viruses and viroids in chrysanthemum.
abstract: Chrysanthemum plants are subject to serious virus diseases, so detection and identification of virus pathogens is important to prevent the virus spread. A reliable one-step multiplex RT-PCR was developed to simultaneously detect two viruses and two viriods: chrysanthemum virus B, tomato Aspermy virus, chrysanthemum stunt viroid and chrysanthemum chlorotic mottle viroid. In addition, we investigated the detection limit and the efficiency of single and multiplex RT-PCR assays. The results showed that the multiplex RT-PCR assay proved to be as sensitive as the single one. In conclusion, this technique is potentially useful in routine diagnosis of chrysanthemum viruses and viroids.
  
  
  CONCLUSIONS
  The multiplex RT-PCR assay described in this study is the first report of simultaneous detection of virus and viroid in chrysanthemum, which provides a fast, convenient, cost-saving way to detect the virus and viroid mixed infections in plants.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing, China.
authors.forename: A
authors.initials: A
authors.lastname: Song
authors.forename: Y
authors.initials: Y
authors.lastname: You
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.forename: P
authors.initials: P
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Jiang
authors.forename: S
authors.initials: S
authors.lastname: Chen
mesh_terms: D031202:Chrysanthemum / Q000821:virology*; D057230:Limit of Detection; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D010942:Plant Viruses / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014772:Viroids / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral