title: Contrast and stability of the axon diameter index from microstructure imaging with diffusion MRI.
abstract: The ActiveAx technique fits the minimal model of white matter diffusion to diffusion MRI data acquired using optimized protocols that provide orientationally invariant indices of axon diameter and density. We investigated how limitations of the available maximal gradient strength (Gmax) on a scanner influence the sensitivity to a range of axon diameters. Multishell high-angular-diffusion-imaging (HARDI) protocols for Gmax of 60, 140, 200, and 300 mT/m were optimized for the pulsed-gradient-spin-echo (PGSE) sequence. Data were acquired on a fixed monkey brain and Monte-Carlo simulations supported the results. Increasing Gmax reduces within-voxel variation of the axon diameter index and improves contrast beyond what is achievable with higher signal-to-noise ratio. Simulations reveal an upper bound on the axon diameter (∼10 μm) that pulsed-gradient-spin-echo measurements are sensitive to, due to a trade-off between short T2 and the long diffusion time needed to probe larger axon diameters. A lower bound (∼2.5 μm) slightly dependent on Gmax was evident, below which axon diameters are identifiable as small, but impossible to differentiate. These results emphasize the key-role of Gmax for enhancing contrast between axon diameter distributions and are, therefore, relevant in general for microstructure imaging methods and highlight the need for increased Gmax on future commercial systems.
authors.affiliation: Danish Research Centre for Magnetic Resonance, Copenhagen University Hospital Hvidovre, Hvidovre, Denmark.
authors.forename: Tim B
authors.initials: TB
authors.lastname: Dyrby
authors.forename: Lise V
authors.initials: LV
authors.lastname: Sogaard
authors.forename: Matt G
authors.initials: MG
authors.lastname: Hall
authors.forename: Maurice
authors.initials: M
authors.lastname: Ptito
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Alexander
mesh_terms: D000818:Animals; D001369:Axons; D038524:Diffusion Magnetic Resonance Imaging / Q000379:methods*; D000882:Haplorhini; D009010:Monte Carlo Method; D012680:Sensitivity and Specificity