title: Molecular characterization of the viaB locus encoding the biosynthetic machinery for Vi capsule formation in Salmonella Typhi.
abstract: The Vi capsular polysaccharide (CPS) of Salmonella enterica serovar Typhi, the cause of human typhoid, is important for infectivity and virulence. The Vi biosynthetic machinery is encoded within the viaB locus composed of 10 genes involved in regulation of expression (tviA), polymer synthesis (tviB-tviE), and cell surface localization of the CPS (vexA-vexE). We cloned the viaB locus from S. Typhi and transposon insertion mutants of individual viaB genes were characterized in Escherichia coli DH5α. Phenotype analysis of viaB mutants revealed that tviB, tviC, tviD and tviE are involved in Vi polymer synthesis. Furthermore, expression of tviB-tviE in E. coli DH5α directed the synthesis of cytoplasmic Vi antigen. Mutants of the ABC transporter genes vexBC and the polysaccharide copolymerase gene vexD accumulated the Vi polymer within the cytoplasm and productivity in these mutants was greatly reduced. In contrast, de novo synthesis of Vi polymer in the export deficient vexA mutant was comparable to wild-type cells, with drastic effects on cell stability. VexE mutant cells exported the Vi, but the CPS was not retained at the cell surface. The secreted polymer of a vexE mutant had different physical characteristics compared to the wild-type Vi.
authors.affiliation: GlycoVaxyn AG, Schlieren, Switzerland.
authors.forename: Michael
authors.initials: M
authors.lastname: Wetter
authors.forename: David
authors.initials: D
authors.lastname: Goulding
authors.forename: Derek
authors.initials: D
authors.lastname: Pickard
authors.forename: Michael
authors.initials: M
authors.lastname: Kowarik
authors.forename: Charles J
authors.initials: CJ
authors.lastname: Waechter
authors.forename: Gordon
authors.initials: G
authors.lastname: Dougan
authors.forename: Michael
authors.initials: M
authors.lastname: Wacker
mesh_terms: D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012485:Salmonella typhi / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D014774:Virulence
chemical_list: D011135:Polysaccharides, Bacterial