title: Localization-based super-resolution microscopy with an sCMOS camera part II: experimental methodology for comparing sCMOS with EMCCD cameras.
abstract: Nowadays, there is a hot debate among industry and academic researchers that whether the newly developed scientific-grade Complementary Metal Oxide Semiconductor (sCMOS) cameras could become the image sensors of choice in localization-based super-resolution microscopy. To help researchers find answers to this question, here we reported an experimental methodology for quantitatively comparing the performance of low-light cameras in single molecule detection (characterized via image SNR) and localization (via localization accuracy). We found that a newly launched sCMOS camera can present superior imaging performance than a popular Electron Multiplying Charge Coupled Device (EMCCD) camera in a signal range (15-12000 photon/pixel) more than enough for typical localization-based super-resolution microscopy.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Long
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
authors.forename: Zhen-Li
authors.initials: ZL
authors.lastname: Huang
mesh_terms: D004583:Electrons; D005453:Fluorescence; D007091:Image Processing, Computer-Assisted; D008670:Metals / Q000737:chemistry*; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D010087:Oxides / Q000737:chemistry*; D010781:Photography / Q000295:instrumentation*; D017785:Photons; D012666:Semiconductors; D059629:Signal-To-Noise Ratio
chemical_list: D008670:Metals; D010087:Oxides