title: Molecular characterization of the rice protein RSS1 required for meristematic activity under stressful conditions.
abstract: Post embryonic growth of plants depends on cell division activity in the shoot and root meristems, in conjunction with subsequent cell differentiation. Under environmental stress conditions, where plant growth is moderately impaired, the meristematic activity is maintained by mechanisms as yet unknown. We previously showed that the rice protein RSS1, whose stability is regulated depending on the cell cycle phases, is a key factor for the maintenance of meristematic activity under stressful conditions. RSS1 interacts with a catalytic subunit of protein phosphatase 1 (PP1), but other molecular characteristics are largely unknown. Here we show that RSS1 interacts with all the PP1 expressed in the shoot apex of rice. This interaction requires one of the conserved regions of RSS1, which is important for RSS1 function. Interestingly, the recombinant RSS1 protein is highly resistant to heat with respect to its anti-coagulability and binding activity to PP1. The features of RSS1 are reminiscent of those of inhibitor-2 of animals, although it is likely that the mode of function of RSS1 is different from that of inhibitor-2. Noticeably, RSS1 binds to PP1 under extremely high ionic strength conditions in vitro. Therefore, RSS1 possibly functions by forming a stable complex with PP1.
authors.affiliation: Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ogawa
authors.forename: Haruka
authors.initials: H
authors.lastname: Morita
authors.forename: Tsukaho
authors.initials: T
authors.lastname: Hattori
authors.forename: Shin
authors.initials: S
authors.lastname: Takeda
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D006358:Hot Temperature; D018519:Meristem / Q000254:growth & development / Q000378:metabolism*; D012275:Oryza / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D018520:Plant Shoots / Q000254:growth & development / Q000378:metabolism; D011485:Protein Binding; D054645:Protein Phosphatase 1 / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D054645:Protein Phosphatase 1