title: Functional studies of soybean (Glycine max L.) seed LEA proteins GmPM6, GmPM11, and GmPM30 by CD and FTIR spectroscopy.
abstract: The protein and mRNA levels of late embryogenesis abundant (LEA) genes may be linked to osmotic stresses. Here, we characterized three soybean hydrophilic LEA proteins--GmPM11 (LEA I), GmPM6 (LEA II), and GmPM30 (LEA III)--by circular dichroism and Fourier transform infrared spectroscopy. Structural analysis revealed that the LEA proteins adopted high amounts of disordered conformations in solution and underwent conformational changes with hydrophobicity and desiccation induction. Macromolecular interaction studies revealed that the GmPM proteins interact with non-reducing sugars and phospholipids. GmPM6 and GmPM30 but not GmPM11 could prevent beta-aggregation of poly-L-lysine after slow drying. We discuss the possible functions of hydrophilic LEA proteins in maturing seeds.
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
authors.forename: Ming-Der
authors.initials: MD
authors.lastname: Shih
authors.forename: Tzung-Yang
authors.initials: TY
authors.lastname: Hsieh
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Jian
authors.forename: Ming-Tsung
authors.initials: MT
authors.lastname: Wu
authors.forename: Shin-Jin
authors.initials: SJ
authors.lastname: Yang
authors.forename: Folkert A
authors.initials: FA
authors.lastname: Hoekstra
authors.forename: Yue-Ie C
authors.initials: YI
authors.lastname: Hsing
mesh_terms: D002942:Circular Dichroism / Q000379:methods*; D003890:Desiccation; D004591:Electrophoresis, Polyacrylamide Gel; D057927:Hydrophobic and Hydrophilic Interactions; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D011107:Polylysine / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D030262:Soybean Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max / Q000235:genetics / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D013395:Sucrose / Q000737:chemistry / Q000378:metabolism
chemical_list: D018832:Molecular Chaperones; D010743:Phospholipids; D011994:Recombinant Proteins; D030262:Soybean Proteins; D011107:Polylysine; D013395:Sucrose