title: Quantifying and imaging engineered nanomaterials in vivo: challenges and techniques.
abstract: Quantifying and imaging the engineered nanomaterials (ENMs) in vivo can provide information on the bio-distribution and fate of ENMs in living systems. A necessary amount of in vivo quantitative data is indispensable to verify the extrapolation from in vitro tests, to modify the predictive models of ENM exposure, and to underpin the risk management strategy for ENMs. However, it remains a challenge to quantitatively assess the bio-distribution of ENMs under realistic exposure, their long-term deposition (especially in non-targeted tissues), their passage across the natural barriers, and the impacts of nano-bio interactions on their in vivo behaviors. Some commonly used techniques for in vivo ENM quantification, such as electron microscopy, fluorescence-based detection, atomic spectroscopy, radiotracing, and techniques basing on synchrotron radiation are reviewed, and their technical characteristics, the state of the art, limitations, and future prospects are addressed.
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences (CAS), Beijing 100049, China. hx421@ihep.ac.cn
authors.forename: Xiao
authors.initials: X
authors.lastname: He
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Ma
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D057230:Limit of Detection; D013058:Mass Spectrometry; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008962:Models, Theoretical; D049329:Nanostructures; D017356:Synchrotrons; D014018:Tissue Distribution