title: A comparative analysis of glomerulus development in the pronephros of medaka and zebrafish.
abstract: The glomerulus of the vertebrate kidney links the vasculature to the excretory system and produces the primary urine. It is a component of every single nephron in the complex mammalian metanephros and also in the primitive pronephros of fish and amphibian larvae. This systematic work highlights the benefits of using teleost models to understand the pronephric glomerulus development. The morphological processes forming the pronephric glomerulus are astoundingly different between medaka and zebrafish. (1) The glomerular primordium of medaka - unlike the one of zebrafish - exhibits a C-shaped epithelial layer. (2) The C-shaped primordium contains a characteristic balloon-like capillary, which is subsequently divided into several smaller capillaries. (3) In zebrafish, the bilateral pair of pronephric glomeruli is fused at the midline to form a glomerulus, while in medaka the two parts remain unmerged due to the interposition of the interglomerular mesangium. (4) Throughout pronephric development the interglomerular mesangial cells exhibit numerous cytoplasmic granules, which are reminiscent of renin-producing (juxtaglomerular) cells in the mammalian afferent arterioles. Our systematic analysis of medaka and zebrafish demonstrates that in fish, the morphogenesis of the pronephric glomerulus is not stereotypical. These differences need be taken into account in future analyses of medaka mutants with glomerulus defects.
authors.affiliation: Department of Cell Biology, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma, USA.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Ichimura
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Bubenshchikova
authors.forename: Rebecca
authors.initials: R
authors.lastname: Powell
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Fukuyo
authors.forename: Tomomi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Uyen
authors.initials: U
authors.lastname: Tran
authors.forename: Shoji
authors.initials: S
authors.lastname: Oda
authors.forename: Minoru
authors.initials: M
authors.lastname: Tanaka
authors.forename: Oliver
authors.initials: O
authors.lastname: Wessely
authors.forename: Hidetake
authors.initials: H
authors.lastname: Kurihara
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Sakai
authors.forename: Tomoko
authors.initials: T
authors.lastname: Obara
mesh_terms: D000818:Animals; D003594:Cytoplasmic Granules / Q000502:physiology / Q000648:ultrastructure; D004625:Embryo, Nonmammalian; D029941:Fish Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D007678:Kidney Glomerulus / Q000033:anatomy & histology* / Q000196:embryology / Q000378:metabolism; D051379:Mice; D009024:Morphogenesis / Q000502:physiology; D009990:Oryzias / Q000033:anatomy & histology* / Q000196:embryology / Q000235:genetics; D060910:Pronephros / Q000033:anatomy & histology* / Q000196:embryology / Q000378:metabolism; D051381:Rats; D012083:Renin / Q000235:genetics*; D013045:Species Specificity; D015027:Zebrafish / Q000033:anatomy & histology* / Q000196:embryology / Q000235:genetics
chemical_list: D029941:Fish Proteins; D012083:Renin