title: Microbial cocktail for bioconversion of green waste to reducing sugars.
abstract: Green waste has been identified as a sustainable resource to convert into reducing sugars and subsequently for production of ethanol. In this study, enhancement of reducing sugar production from green waste by the different combination of pure strains was investigated. The best-defined microbial cocktail for high reducing sugars production, consisting of one fungus (Pseudallescheria sp. D42) and three bacteria (Microbacterium sp. F28, Tsukamurella sp. C35, and Bacillus sp. F4), was successfully constructed. The maximum reducing sugars yield by this fungal-bacterial cocktail was 165.2 mg/g-green waste within 24 h, which is approximate 10 times higher than the selected individual microbial strains. Without extraction and purification of specific enzymes, whole-cell-bioconversion by a defined microbial cocktail is proven as a potential alternative process for lignocellulose hydrolysis and reducing sugars production.
authors.affiliation: Residues and Resource Reclamation Centre, Nanyang Environment and Water Research Institute, #06-08 CleanTech One, 1 Cleantech Loop, Singapore 637141, Singapore. clchen@ntu.edu.sg
authors.forename: Chia-Lung
authors.initials: CL
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Wang
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001711:Biotransformation; D050260:Carbohydrate Metabolism; D002241:Carbohydrates; D000431:Ethanol / Q000378:metabolism / Q000600:supply & distribution; D055772:Green Chemistry Technology; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D010084:Oxidation-Reduction; D011541:Pseudallescheria / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002241:Carbohydrates; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin