title: Quantification of diadenosine polyphosphates in blood plasma using a tandem boronate affinity-ion exchange chromatography system.
abstract: Endogenous diadenosine polyphosphates (Ap(n)As) have been associated with a variety of biological effects but quantifying their concentration in blood is difficult. We report on the development of a tandem affinity-ion exchange high-performance liquid chromatography (HPLC) system that employs boronate affinity upstream of ion exchange chromatography for automated rapid (45-min) resolution and extraction of Ap(n)As from human plasma. This system obviates previous requirements for multiple column separations and handling steps, so it is ideally set up for time- and cost-efficient screening of blood samples for Ap(n)A pharmacokinetic and biodistribution studies.
authors.affiliation: Institute of Pharmaceutical Science, King's College London, London SE1 9NH, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Wright
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Miller
mesh_terms: D001897:Boronic Acids / Q000737:chemistry*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D015226:Dinucleoside Phosphates / Q000097:blood*; D006801:Humans
chemical_list: D001897:Boronic Acids; D015226:Dinucleoside Phosphates