title: A heat-inducible CRE/LOXP gene induction system in medaka.
abstract: We established three lines of transgenic medaka, a heat-shock element (HSE) monitor line (hse-GFP line), heat-inducible driver lines (hse-cre lines), and effector lines (gapdh-loxP[DsRed]-GFP lines). We employed these to comprehensively analyze gene induction at different time points in various tissues. These analyses demonstrate a good response of synthetic HSEs by heat treatment during embryogenesis and the mosaic gene induction by cre/loxP-mediated recombination, thus providing practical information regarding the feasibility of a heat-inducible cre/loxP-mediated system in medaka. We also activated recombination by local heat-treatment using a metal probe and an infrared laser. Our results collectively indicate that these lines allow us to perform lineage tracing and mosaic analysis and provide the platform to investigate gene functions at later developmental stage and adult.
authors.affiliation: Laboratory of Molecular Genetics for Reproduction, National Institute for Basic Biology, Okazaki 444-8787, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Kobayashi
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kamei
authors.forename: Masato
authors.initials: M
authors.lastname: Kinoshita
authors.forename: Thomas
authors.initials: T
authors.lastname: Czerny
authors.forename: Minoru
authors.initials: M
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D018390:Gene Targeting; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics; D006358:Hot Temperature; D019426:Integrases / Q000235:genetics*; D009990:Oryzias / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: D006360:Heat-Shock Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C045073:Cre recombinase; D019426:Integrases