title: Structures of a γ-aminobutyrate (GABA) transaminase from the s-triazine-degrading organism Arthrobacter aurescens TC1 in complex with PLP and with its external aldimine PLP-GABA adduct.
abstract: Two complex structures of the γ-aminobutyrate (GABA) transaminase A1R958 from Arthrobacter aurescens TC1 are presented. The first, determined to a resolution of 2.80 Å, features the internal aldimine formed by reaction between the ℇ-amino group of Lys295 and the cofactor pyridoxal phosphate (PLP); the second, determined to a resolution of 2.75 Å, features the external aldimine adduct formed between PLP and GABA in the first half-reaction. This is the first structure of a microbial GABA transaminase in complex with its natural external aldimine and reveals the molecular determinants of GABA binding in this enzyme.
authors.affiliation: York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, England.
authors.forename: Heather
authors.initials: H
authors.lastname: Bruce
authors.forename: Anh
authors.initials: A
authors.lastname: Nguyen Tuan
authors.forename: Juan
authors.initials: J
authors.lastname: Mangas Sánchez
authors.forename: Charlotte
authors.initials: C
authors.lastname: Leese
authors.forename: Jennifer
authors.initials: J
authors.lastname: Hopwood
authors.forename: Ralph
authors.initials: R
authors.lastname: Hyde
authors.forename: Sam
authors.initials: S
authors.lastname: Hart
authors.forename: Johan P
authors.initials: JP
authors.lastname: Turkenburg
authors.forename: Gideon
authors.initials: G
authors.lastname: Grogan
mesh_terms: D000612:4-Aminobutyrate Transaminase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics; D008958:Models, Molecular; D010802:Phylogeny; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D011732:Pyridoxal Phosphate / Q000737:chemistry* / Q000378:metabolism; D040681:Structural Homology, Protein
chemical_list: D011732:Pyridoxal Phosphate; D000612:4-Aminobutyrate Transaminase