title: Establishing causality for dopamine in neural function and behavior with optogenetics.
abstract: Dopamine (DA) is known to play essential roles in neural function and behavior. Accordingly, DA neurons have been the focus of intense experimental investigation that has led to many important advances in our understanding of how DA influences these processes. However, it is becoming increasingly appreciated that delineating the precise contributions of DA neurons to cellular, circuit, and systems-level phenomena will require more sophisticated control over their patterns of activity than conventional techniques can provide. Specifically, the roles played by DA neurons are likely to depend on their afferent and efferent connectivity, the timing and length of their neural activation, and the nature of the behavior under investigation. Recently developed optogenetic tools hold great promise for disentangling these complex issues. Here we discuss the use of light-sensitive microbial opsins in the context of outstanding questions in DA research. A major technical advance offered by these proteins is the ability to bidirectionally modulate DA neuron activity in in vitro and in vivo preparations on a time scale that more closely approximates those of neural, perceptual and behavioral events. In addition, continued advances in rodent genetics and viral-mediated gene delivery have contributed to the ability to selectively target DA neurons or their individual afferent and efferent connections. Further, these tools are suitable for use in experimental subjects engaged in complex behaviors. After reviewing the strengths and limitations of optogenetic methodologies, we conclude by describing early efforts in the application of this valuable new approach that demonstrate its potential to improve our understanding of the neural and behavioral functions of DA. This article is part of a Special Issue entitled Optogenetics (7th BRES).
authors.affiliation: Ernest Gallo Clinic and Research Center, University of California, San Francisco, Emeryville, CA 94608, USA.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Steinberg
authors.forename: Patricia H
authors.initials: PH
authors.lastname: Janak
mesh_terms: D000200:Action Potentials / Q000235:genetics / Q000502:physiology; D000818:Animals; D001519:Behavior; D001921:Brain / Q000166:cytology; D015984:Causality; D004298:Dopamine / Q000235:genetics / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000502:physiology*; D062308:Optogenetics; D010775:Photic Stimulation
chemical_list: D004298:Dopamine