title: Adaptive optics enables 3D STED microscopy in aberrating specimens.
abstract: Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue.
authors.affiliation: Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA.
authors.forename: Travis J
authors.initials: TJ
authors.lastname: Gould
authors.forename: Daniel
authors.initials: D
authors.lastname: Burke
authors.forename: Joerg
authors.initials: J
authors.lastname: Bewersdorf
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Booth
mesh_terms: D000818:Animals; D008856:Microscopy, Fluorescence / Q000379:methods*; D008863:Microspheres; D055620:Optical Phenomena; D055095:Optics and Photonics / Q000379:methods*; D012160:Retina / Q000033:anatomy & histology; D015027:Zebrafish