title: Expression of AEG-1 in human T-cell lymphoma enhances the risk of progression.
abstract: The aim of this study was to examine the expression and role of astrocyte             elevated gene-1 (AEG-1) in biological processes of T-cell non-Hodgkin's lymphoma             (T-NHL). AEG-1 expression in T-NHL patients was characterized with immunohistochemistry.             The expression of AEG-1, survivin, Bcl-2 and Bax in Jurkat and Hut-78 cells was             detected by real-time PCR and western blotting. Cell proliferation, cell cycle             and apoptosis were measured by MTT and flow cytometry. MMP-2/-9 activity was detected             by gelatin zymography. Of the studied tumors, 104 (80.62%) exhibited cytoplasmic             AEG-1 immunostaining. AEG-1-siRNA in Jurkat and Hut-78 cells suppressed cell proliferation             and induced cell apoptosis, inhibited survivin and Bcl-2/Bax protein expression             as well as MMP-2/-9 activity. Downregulation of AEG-1 using siRNA could provide             a potential approach for gene therapy against T-NHL, and the antitumor effects             may be associated with inhibition of survivin and Bcl-2/Bax protein expression             and MMP-2/-9 activity.
authors.affiliation: Department of Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, PR China.
authors.forename: Jiaqin
authors.initials: J
authors.lastname: Yan
authors.forename: Mingzhi
authors.initials: M
authors.lastname: Zhang
authors.forename: Qingjiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism; D019169:Jurkat Cells; D016399:Lymphoma, T-Cell / Q000235:genetics* / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008565:Membrane Proteins; D034622:RNA Interference; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D000077022:Survivin; D050998:bcl-2 Homologous Antagonist-Killer Protein / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C107489:BIRC5 protein, human; D015815:Cell Adhesion Molecules; D051034:Inhibitor of Apoptosis Proteins; C483039:MTDH protein, human; D008565:Membrane Proteins; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; D000077022:Survivin; D050998:bcl-2 Homologous Antagonist-Killer Protein; D051028:bcl-2-Associated X Protein; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9