title: Measurement of glutathione reductase activity.
abstract: Reduced glutathione, a thiol, is essential to the survival of the cells of most aerobic organisms. It is present intracellularly and provides protection from hydroperoxides and free radicals. This unit describes a continuous spectrophotometric assay for reductase activity: it follows the reduction of glutathione disulfide to reduced glutathione by monitoring the oxidation of NADPH as visualized by a decrease in absorbance at 340 nm.
authors.affiliation: Uppsala University, Uppsala, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Mannervik
mesh_terms: D000818:Animals; D003600:Cytosol / Q000201:enzymology; D057075:Enzyme Assays / Q000379:methods*; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000032:analysis*; D006801:Humans
chemical_list: D005980:Glutathione Reductase; D005978:Glutathione