title: Development of PCR internal controls for DNA profiling with the AmpFℓSTR® SGM Plus® amplification kit.
abstract: Forensic DNA profiling uses a series of commercial kits that co-amplify several loci in one reaction; the products of the PCR are fluorescently labelled and analysed using CE. Before CE, an aliquot of the PCR is mixed with formamide and an internal lane size standard. Using the SGM Plus amplification kit, we have developed two internal non-amplified controls of 80 bp and 380 bp that are labelled with ROX fluorescent dye and added to the PCR. Combined with two internal amplification controls of 90 bp and 410 bp, they provide additional controls for the PCR, electrokinetic injection, and CE and also function as an internal size standard.
authors.affiliation: School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, UK.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Nathalie
authors.forename: Sibte
authors.initials: S
authors.lastname: Hadi
authors.forename: William
authors.initials: W
authors.lastname: Goodwin
mesh_terms: D004247:DNA / Q000032:analysis / Q000737:chemistry; D016172:DNA Fingerprinting / Q000379:methods / Q000592:standards*; D060005:Genotyping Techniques / Q000379:methods / Q000592:standards; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012015:Reference Standards
chemical_list: D011933:Reagent Kits, Diagnostic; D004247:DNA