title: Chronic effects of pulmonary artery stenosis on hemodynamic and structural development of the lungs.
abstract: Pulmonary artery (PA) stenosis is a difficult obstructive defect to manage since clinicians cannot know a priori which obstructions to treat and when. Prognosis of PA stenosis and its chronic effects on lung development are poorly understood. This study aimed to characterize the hemodynamic and structural effects of PA stenosis during development. Fourteen male Sprague-Dawley rats underwent left PA (LPA) banding at age 21 days, and 13 underwent sham operation. Hemodynamic and structural impacts were studied longitudinally at 20, 36, 52, 100, and 160 days. Chronic LPA banding resulted in a significant reduction in LPA flow (P < 0.0001) and size of both proximal LPA (P < 0.0001) and distal LPA (P < 0.01), as well as a significant increase in flow and size of the right PA (P < 0.05) throughout development. Flows and sizes adapted such that normal levels of wall shear were restored after banding. At 160 days, LPA banding resulted in a significant decrease in left lung volume and an increase in right lung volume but no significant differences in total lung volume. There was an elevation of proximal LPA pressure as well as right ventricular hypertrophy in the banded animals. The banded lung exhibited arterial disorganization, loss of vessels, and enlargement of its bronchial arteries, whereas the contralateral lung showed signs of vascular pathology. There are consequences on development of both lungs in the presence of an LPA stenosis at young age. These results suggest that early intervention may be necessary to optimize left lung growth and minimize right lung vascular pathology.
authors.affiliation: Department of Bioengineering, Stanford University, California 94305-5444, USA. razavih@alumni.stanford.edu
authors.forename: Hedi
authors.initials: H
authors.lastname: Razavi
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Stewart
authors.forename: Chengpei
authors.initials: C
authors.lastname: Xu
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sawada
authors.forename: Shahrzad Y
authors.initials: SY
authors.lastname: Zarafshar
authors.forename: Charles A
authors.initials: CA
authors.lastname: Taylor
authors.forename: Marlene
authors.initials: M
authors.lastname: Rabinovitch
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Feinstein
mesh_terms: D000818:Animals; D006439:Hemodynamics; D017380:Hypertrophy, Right Ventricular / Q000473:pathology; D008026:Ligation; D008168:Lung / Q000254:growth & development*; D008297:Male; D011651:Pulmonary Artery / Q000473:pathology* / Q000503:physiopathology; D011652:Pulmonary Circulation; D011666:Pulmonary Valve Stenosis / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley