title: ICESp1116, the genetic element responsible for erm(B)-mediated, inducible resistance to erythromycin in Streptococcus pyogenes.
abstract: ICESp1116, responsible for erm(B)-mediated, inducible erythromycin resistance in Streptococcus pyogenes, was comprehensively characterized, and its chromosomal integration site was determined. It displayed a unique mosaic organization consisting of a scaffold, related to TnGallo1 from Streptococcus gallolyticus, with two inserted fragments separated by IS1216. One fragment, containing erm(B), displayed high-level identity to a portion of the S. pyogenes plasmid pSM19035; the other, containing a truncated tet(M) gene, displayed high-level identity to the right-hand portion of Clostridium difficile Tn5397.
authors.affiliation: Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Brenciani
authors.forename: Erika
authors.initials: E
authors.lastname: Tiberi
authors.forename: Eleonora
authors.initials: E
authors.lastname: Morici
authors.forename: Erman
authors.initials: E
authors.lastname: Oryasin
authors.forename: Eleonora
authors.initials: E
authors.lastname: Giovanetti
authors.forename: Pietro E
authors.initials: PE
authors.lastname: Varaldo
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D016360:Clostridioides difficile / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D013291:Streptococcus / Q000187:drug effects / Q000235:genetics; D013297:Streptococcus pyogenes / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C551474:ermB protein, Streptococcus pyogenes; D004917:Erythromycin