title: Exploratory study of Torque teno sus viruses in pulmonary inflammatory lesions in pigs.
abstract: The pathogenic role of Torque teno sus viruses 1 (TTSuV1) and 2 (TTSuV2), and their capacity to induce lesions are controversial. TTSuVs have been linked to porcine circovirus diseases (PCVDs) and described as cause of mild respiratory lesions in gnotobiotic pigs; moreover, an increased TTSuV prevalence has been found in porcine respiratory disease complex (PRDC) affected pigs. In the present study, TTSuV1 and TTSuV2 loads and their prevalence were evaluated in formalin-fixed, paraffin-embedded (FFPE) lung samples displaying different types of inflammatory lesions. Such measurements were made by means of a real time quantitative PCR technique to detect these viruses. The technique was optimised for its use on FFPE tissues comparing results with frozen lung tissues. Selection criteria included negativity against porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), Aujeszky's disease virus (ADV) and swine influenza virus (SIV). Results from the present study demonstrated that both TTSuVs were present in lung. However, TTSuV2 had higher viral load and prevalence in all the studied groups when compared to TTSuV1. TTSuV2 mean load was also higher in lungs with viral background (interstitial pneumonia and broncho-interstitial pneumonia) when compared to normal lungs or to those with bacterial background (catarrhal-purulent bronchopneumonia, fibrinous pleuritis and fibrinous-necrotizing pleuropneumonia). This result suggests a possible role of TTSuV2 in the pathogenic mechanism of inflammatory lesions of lungs compatible with viral infection.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: mario.aramouni@cresa.uab.cat.
authors.forename: M
authors.initials: M
authors.lastname: Aramouni
authors.affiliation: Departament de Sanitat i Anatomia Animals, Facultat Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Martínez
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Nieto
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Kekarainen
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain; Departament de Sanitat i Anatomia Animals, Facultat Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: joaquim.segales@cresa.uab.es.
authors.forename: J
authors.initials: J
authors.lastname: Segalés
mesh_terms: D000818:Animals; D004266:DNA Virus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D008168:Lung / Q000821:virology; D016612:Paraffin Embedding; D011024:Pneumonia, Viral / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D013030:Spain / Q000453:epidemiology; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D022783:Torque teno virus / Q000235:genetics / Q000302:isolation & purification*; D019562:Viral Load