title: Hepatocurative activity of biosynthesized silver nanoparticles fabricated using Andrographis paniculata.
abstract: The current investigation was aimed to determine the hepatocurative role of silver nanoparticles (AgNPs) synthesized rapidly using Andrographis paniculata. The nanoparticles fabricated at varying temperatures were characterized by UV-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), fourier transform infra-red spectroscopy (FTIR), energy dispersive X-ray (EDX) and inductively coupled plasma optical emission spectroscopy (ICP-OES) alongside zeta potential measurement. UV-vis spectroscopic readings indicated a prominent peak at 423 nm. TEM analysis indicated that the biosynthesized nanospheres were in the size range of 13-27 nm. EDX spectrum indicated strong signal for AgNPs with 90.1% purity. The total concentration of AgNps was 216.7 mg/L after synthesis as by ICP-OES. Zeta potential was -34.3 mV indicating stable AgNPs. In vitro radical scavenging assay proved strong antioxidant effect of the AgNPs compared to 5% aqueous leaf extract. CCl(4) was used to induce hepatic injury in mice model. The biosynthesized AgNPs at three different doses (25, 50, 100mg/kg BW of the animal) were used for treatment. Silymarin was used as a standard. Low dose (25mg/kg BW) was effective in revival of all biological parameters to near normal in all intoxicated groups indicating the curing effects on CCl(4) induced liver injury.
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 24, Tamil Nadu, India.
authors.forename: Udhayaraj
authors.initials: U
authors.lastname: Suriyakalaa
authors.forename: Jacob Joe
authors.initials: JJ
authors.lastname: Antony
authors.forename: Subramanian
authors.initials: S
authors.lastname: Suganya
authors.forename: Durairaj
authors.initials: D
authors.lastname: Siva
authors.forename: Raman
authors.initials: R
authors.lastname: Sukirtha
authors.forename: Soundarrajan
authors.initials: S
authors.lastname: Kamalakkannan
authors.forename: P B Tirupathi
authors.initials: PB
authors.lastname: Pichiah
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Achiraman
mesh_terms: D035685:Andrographis / Q000737:chemistry*; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use / Q000648:ultrastructure; D051379:Mice; D046529:Microscopy, Electron, Transmission; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D012834:Silver