title: Quantitative determination of paclitaxel and its metabolites, 6α-hydroxypaclitaxel and p-3'-hydroxypaclitaxel, in human plasma using column-switching liquid chromatography/tandem mass spectrometry.
abstract: A column-switching liquid chromatography/electrospray ionization tandem mass spectrometry to determine paclitaxel and its metabolites, 6α-hydroxypaclitaxel and p-3'-hydroxypaclitaxel, in human plasma was developed. The analytical system had a Shim-Pack MAYI-ODS (10 × 4.6 mm i.d.) trapping column with deproteinization ability that concentrates analytes and removes water-soluble components. This method covered a linearity range of 5-5000 ng/mL of concentrations in plasma for paclitaxel, a range of 0.87-870 ng/mL for 6α-hydroxypaclitaxel and a range of 0.87-435 ng/mL for p-3'-hydroxypaclitaxel. The intra-day precision and inter-day precision of analysis were less than 11.1%, and the accuracy was within ±14.4% at concentrations of 5, 50, 500 and 5000 ng/mL for paclitaxel, 0.87, 8.7, 87 and 870 ng/mL for 6α-hydroxypaclitaxel, and 0.87, 8.7, 87 and 435 ng/mL for p-3'-hydroxypaclitaxel. The total run time was 30 min. Our method was successfully applied to clinical pharmacokinetic investigation.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Asuka
authors.initials: A
authors.lastname: Fujikawa
authors.forename: Hajime
authors.initials: H
authors.lastname: Ito
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Tanaka
authors.forename: Ayako
authors.initials: A
authors.lastname: Furugen
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Miyamori
authors.forename: Natsuko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Jiro
authors.initials: J
authors.lastname: Ogura
authors.forename: Masaki
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Takehiro
authors.initials: T
authors.lastname: Yamada
authors.forename: Nariyasu
authors.initials: N
authors.lastname: Mano
authors.forename: Ken
authors.initials: K
authors.lastname: Iseki
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000097:blood*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D016903:Drug Monitoring / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D008175:Lung Neoplasms / Q000188:drug therapy; D017239:Paclitaxel / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D043823:Taxoids / Q000097:blood*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D043823:Taxoids; C102050:3'-p-hydroxypaclitaxel; C086219:6-hydroxytaxol; D017239:Paclitaxel