title: Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers.
abstract: Fatty acid translocase (FAT/CD36) is a membrane receptor that facilitates long-chain fatty acid uptake. To investigate its role in the regulation of long-chain fatty acid composition in muscle tissue, we studied and compared FAT/CD36 gene expression in muscle tissues of commercial broiler chickens and Chinese local Silky fowls. The results from gas chromatography-mass spectrometry analysis of muscle samples demonstrated that Chinese local Silky fowls had significantly higher (P < 0.05) proportions of linoleic acid (LA) and palmitic acid, lower proportions (P < 0.05) of arachidonic acid (AA) and oleic acid than the commercial broiler chickens. The mRNA expression levels of fatty acid (FA) transporters (FA transport protein-1, membrane FA-binding protein, FAT/CD36 and caveolin-1) in the m. ipsilateral pectoralis and biceps femoris were analyzed by Q-PCR, and FAT/CD36 expression levels showed significant differences between these types of chickens (P < 0.01). Interestingly, the levels of FAT/CD36 expression are positively correlated with LA content (r = 0.567, P < 0.01) but negatively correlated with palmitic acid content (r = -0.568, P < 0.01). Further experiments in the stably transfected Chinese hamster oocytes cells with chicken FAT/CD36 cDNA demonstrated that overexpression of FAT/CD36 improves total FA uptake with a significant increase in the proportion of LA and AA, and a decreased proportion of palmitic acid. These results suggest that chicken FAT/CD36 may selectively transport LA and AA, which may lead to the higher LA deposition in muscle tissue.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou, PR China.
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: G
authors.initials: G
authors.lastname: Shu
authors.forename: L
authors.initials: L
authors.lastname: Zhou
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: W
authors.initials: W
authors.lastname: Liao
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: G
authors.initials: G
authors.lastname: Zhou
authors.forename: Q
authors.initials: Q
authors.lastname: Xi
authors.forename: P
authors.initials: P
authors.lastname: Gao
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: L
authors.initials: L
authors.lastname: Yuan
authors.forename: Q
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001692:Biological Transport; D018955:CD36 Antigens / Q000378:metabolism*; D016466:CHO Cells; D002645:Chickens / Q000378:metabolism*; D002681:China; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005786:Gene Expression Regulation / Q000502:physiology*; D019787:Linoleic Acid / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D019301:Oleic Acid / Q000378:metabolism; D019308:Palmitic Acid / Q000378:metabolism; D016133:Polymerase Chain Reaction; D013045:Species Specificity
chemical_list: D018955:CD36 Antigens; D017931:DNA Primers; D005227:Fatty Acids; D016718:Arachidonic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; D019787:Linoleic Acid