title: Technical improvement of the TBP (tubulin-based polymorphism) method for plant species detection, based on capillary electrophoresis.
abstract: Nowadays, feed and food safety and traceability are of primary importance. Hence, a correct labeling of the different products is highly desirable in general, but mandatory for those people who are suffering from eating disorders and food allergies. Among the technologies that have been developed for feed and food analysis, the patented tubulin-based polymorphism (TBP) method emerges as an easy, versatile, and inexpensive diagnostic tool. Initially used to fingerprint different plant species and varieties, TBP was then successfully applied to trace species in mixtures of plant origin such as commercial feeds. TBP is a DNA-based molecular marker, that makes use of PCR for the selective amplification of plant β-tubulin introns. Amplified fragments are then separated by PAGE and visualized by silver staining. We have now developed an improved version of TBP. Based on capillary electrophoresis and fluorescence detection, it makes the method automatic, more sensible, reproducible, and faster. Compared to the classic TBP, this new version allows to obtain a better data resolution and an easier interpretation of the results, clearing the way to large-scale feed/food diagnostics.
authors.affiliation: Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Milan, Italy.
authors.forename: Floriana
authors.initials: F
authors.lastname: Gavazzi
authors.forename: Anna Paola
authors.initials: AP
authors.lastname: Casazza
authors.forename: Claudia
authors.initials: C
authors.lastname: Depedro
authors.forename: Francesco
authors.initials: F
authors.lastname: Mastromauro
authors.forename: Diego
authors.initials: D
authors.lastname: Breviario
mesh_terms: D000821:Animal Feed / Q000032:analysis; D018744:DNA, Plant / Q000032:analysis / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D017343:Genes, Plant; D005819:Genetic Markers / Q000235:genetics*; D007438:Introns; D000455:Medicago sativa / Q000145:classification / Q000235:genetics; D010944:Plants / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D014404:Tubulin / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D014404:Tubulin