title: [Changes of left ventricular myocardial collagen fibers and osteopontin expression in hypertrophic cardiomyopathy].
abstract: OBJECTIVE
  To investigate the changes of collagen fibers and the expression of osteopontin in the left ventricle in cases of hypertrophic cardiomyopathy (HCM), along with the significance of their potential forensic application.
  
  
  METHODS
  Fifteen cases of HCM, 15 cases of coronary heart disease with cardiac hypertrophy and 20 cases of traffic accidents were selected as HCM group, coronary heart disease group and control group, respectively. Collagen volume fraction and osteopontin expression were observed and compared by HE staining, Masson trichrome staining and immunohistochemistry methods. Imaging and statistical methods were used for quantitative analysis.
  
  
  RESULTS
  Collagen volume fraction in left ventricle of HCM and coronary heart disease were significantly higher than that in the control group (P < 0.05), which was not significantly different between the HCM group and the coronary heart disease group. The integral light density value of osteopontin in left ventricular cardiomyocytes of the HCM group and the coronary heart disease group were significantly higher than that of the control group (P< 0.05), and the value of the HCM group was also significantly higher than that of coronary heart disease group (P < 0.05).
  
  
  CONCLUSIONS
  The increased contents of collagen fibers and the overexpression of osteopontin may play an important role in myocardial fibrosis, and they can be used as markers in aid of diagnosing sudden death due to HCM.
authors.affiliation: Department of Forensic Medicine, Zhongshan Medical College, SunYat-sen University, Guangzhou 510080, China. 41493256@qq.com
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Tang
authors.forename: Guo-Sheng
authors.initials: GS
authors.lastname: Lin
authors.forename: Jing-Lu
authors.initials: JL
authors.lastname: Huang
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Bo
authors.initials: B
authors.lastname: Hao
authors.forename: Yan-Geng
authors.initials: YG
authors.lastname: Yu
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Kong
authors.forename: Li
authors.initials: L
authors.lastname: Quan
authors.forename: Xin-Biao
authors.initials: XB
authors.lastname: Liao
authors.forename: Bin
authors.initials: B
authors.lastname: Luo
mesh_terms: D002312:Cardiomyopathy, Hypertrophic / Q000175:diagnosis* / Q000378:metabolism / Q000503:physiopathology; D016022:Case-Control Studies; D003094:Collagen / Q000378:metabolism; D003327:Coronary Disease / Q000175:diagnosis* / Q000378:metabolism / Q000503:physiopathology; D016757:Death, Sudden, Cardiac / Q000209:etiology; D005260:Female; D005355:Fibrosis; D049429:Forensic Pathology; D006352:Heart Ventricles / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D053495:Osteopontin / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: C506895:SPP1 protein, human; D053495:Osteopontin; D003094:Collagen