title: The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis.
abstract: Arbuscular mycorrhiza (AM) represents an ancient endosymbiosis between plant roots and Glomeromycota fungi. Strigolactones (SLs), plant-derived terpenoid lactones, activate hyphal branching of AM fungi before physical contact. Lack of SL biosynthesis results in lower colonization of AM fungi. The F-box protein, DWARF3 (D3), and the hydrolase family protein DWARF14 (D14) are crucial for SL responses in rice. Here we conducted AM fungal colonization assays with the SL-insensitive d3 and d14 mutants. The d3 mutant exhibited strong defects in AM fungal colonization, whereas the d14 mutant showed higher AM fungal colonization. As D14 has a homologous protein, D14-LIKE, we generated D14-LIKE knockdown lines by RNA interference in the wildtype and d14 background. D14 and D14-LIKE double knockdown lines exhibited similar colonization rates as those of the d14-1 mutant. D3 is crucial for establishing AM symbiosis in rice, whereas D14 and D14-LIKE are not. Our results suggest distinct roles for these SL-related components in AM symbiosis.
authors.affiliation: Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Hiromu
authors.initials: H
authors.lastname: Kameoka
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Nakaku, Sakai, Osaka, 599-8531, Japan.
authors.forename: Misaki
authors.initials: M
authors.lastname: Tempo
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Nakaku, Sakai, Osaka, 599-8531, Japan.
authors.forename: Kohki
authors.initials: K
authors.lastname: Akiyama
authors.affiliation: Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan.|Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Ora-gun, Gunma, 374-0193, Japan.
authors.forename: Mikihisa
authors.initials: M
authors.lastname: Umehara
authors.affiliation: Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan.|Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi, 980-8577, Japan.
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Yamaguchi
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Nakaku, Sakai, Osaka, 599-8531, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Hayashi
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Kyozuka
authors.affiliation: Plant Science Center, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Shirasu
mesh_terms: D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D055137:Glomeromycota / Q000502:physiology*; D007783:Lactones / Q000378:metabolism*; D009154:Mutation; D038821:Mycorrhizae / Q000502:physiology*; D012275:Oryza / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D013559:Symbiosis / Q000502:physiology*; D013729:Terpenes / Q000378:metabolism*
chemical_list: D044783:F-Box Proteins; D007783:Lactones; D010940:Plant Proteins; D013729:Terpenes