title: Relationship between NADPH oxidase p22phox C242T, PARP-1 Val762Ala polymorphisms, angiographically verified coronary artery disease and myocardial infarction in South Indian patients with type 2 diabetes mellitus.
abstract: BACKGROUND
  There has been compelling evidence for the role of oxidative stress in the pathogenesis of cardiovascular complications in type 2 diabetes mellitus (T2DM). We analyzed the association of C242T and Val762Ala polymorphisms of NADPH oxidase p22phox and poly (ADP-ribose) polymerase-1 (PARP-1) genes respectively with coronary artery disease (CAD) and its severity, myocardial infarction (MI) and cardiovascular risk factors in T2DM patients.
  
  
  METHODS
  We screened 283 T2DM patients, inclusive of 160 with angiographically defined CAD, 73 with and 89 without MI and 121 T2DM individuals with no evidence of CAD for the two gene polymorphisms.
  
  
  RESULTS
  The 242T and 762Ala alleles were significantly more frequent in T2DM subjects without CAD than those with CAD, thereby associating them with a significant protective effect against development of CAD [p=0.002 (C242T); 0.02 (Val762Ala)]. The association was further characterized by a relatively lower frequency of 242T and 762Ala alleles in T2DM patients with multi (MVD)/triple vessel disease respectively [p=0.003 (C242T); 0.02 (Val762Ala)]. Conversely, the genotype and allele frequencies of these polymorphisms were not significantly different in T2DM+CAD patients with or without MI. Stratification of risk by putative risk factors for CAD revealed a significant interaction with these polymorphisms. Multiple logistic regression analysis revealed a significant and independent association of C242T and Val762Ala polymorphisms and other putative risk factors with CAD/MVD in T2DM individuals.
  
  
  CONCLUSIONS
  Our observations indicate a significant relationship between p22phox C242T and PARP-1 Val762Ala polymorphisms, CAD and its severity, but not with occurrence of MI in T2DM individuals with significant coronary stenoses.
authors.affiliation: Department of Genetics, Osmania University, Hyderabad, India. parimala_narne@yahoo.co.in
authors.forename: Parimala
authors.initials: P
authors.lastname: Narne
authors.forename: Kamakshi Chaithri
authors.initials: KC
authors.lastname: Ponnaluri
authors.forename: Shailender
authors.initials: S
authors.lastname: Singh
authors.forename: Mohammed
authors.initials: M
authors.lastname: Siraj
authors.forename: Mohammed
authors.initials: M
authors.lastname: Ishaq
mesh_terms: D000792:Angiography; D044466:Asian People; D003324:Coronary Artery Disease / Q000175:diagnosis / Q000201:enzymology* / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology* / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005788:Gene Pool; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D009203:Myocardial Infarction / Q000201:enzymology* / Q000235:genetics; D019255:NADPH Oxidases / Q000235:genetics*; D018384:Oxidative Stress / Q000502:physiology; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D019255:NADPH Oxidases; C086986:CYBA protein, human; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases