title: Evaluation of six commercial DNA extraction kits for recovery of Burkholderia pseudomallei DNA.
abstract: Six commercially available DNA extraction kits, as well as thermal lysis and proteinase K DNA extraction were evaluated regarding bacterial inactivation, DNA yield and purity, and their use in a Burkholderia pseudomallei real-time PCR. While all methods successfully inactivated the bacteria, by measuring DNA purity and the level of detection by real-time PCR, the proteinase K method was the most sensitive.
authors.affiliation: Bavarian Health and Food Safety Authority, Oberschleißheim, Germany. angela.marques@colostate.edu
authors.forename: Maria Angela de Mello
authors.initials: MA
authors.lastname: Marques
authors.forename: Pia
authors.initials: P
authors.lastname: Zimmermann
authors.forename: Ute
authors.initials: U
authors.lastname: Messelhäußer
authors.forename: Andreas
authors.initials: A
authors.lastname: Sing
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D016957:Burkholderia pseudomallei / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D019286:Endopeptidase K / Q000737:chemistry; D006358:Hot Temperature; D011933:Reagent Kits, Diagnostic / Q000191:economics; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; D019286:Endopeptidase K