title: Image analyzing method to evaluate in situ bioluminescence from an obligate anaerobe cultivated under various dissolved oxygen concentrations.
abstract: An image analyzing method was developed to evaluate in situ bioluminescence expression, without exposing the culture sample to the ambient oxygen atmosphere. Using this method, we investigated the effect of dissolved oxygen concentration on bioluminescence from an obligate anaerobe Bifidobacterium longum expressing bacterial luciferase which catalyzes an oxygen-requiring bioluminescent reaction.
authors.affiliation: Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Ninomiya
authors.forename: Ryuji
authors.initials: R
authors.lastname: Yamada
authors.forename: Masami
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Satoru
authors.initials: S
authors.lastname: Fukiya
authors.forename: Takane
authors.initials: T
authors.lastname: Katayama
authors.forename: Chiaki
authors.initials: C
authors.lastname: Ogino
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shimizu
mesh_terms: D001421:Bacteria, Anaerobic / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D001644:Bifidobacterium / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D008156:Luciferases; C071832:alkanol monooxygenase (FMN-linked); D010100:Oxygen