title: Crystallization and preliminary X-ray diffraction analysis of two peptides from Alzheimer PHF in complex with the MN423 antibody Fab fragment.
abstract: The major constituent of the Alzheimer's disease paired helical filaments (PHF) core is the intrinsically disordered protein (IDP) tau. Globular binding partners, e.g. monoclonal antibodies, can stabilize the fold of disordered tau in complexes. A previously published structure of a proteolytically generated tau fragment in a complex with the PHF-specific monoclonal antibody MN423 revealed a turn-like structure of the PHF core C-terminus [Sevcik et al. (2007). FEBS Lett. 581, 5872-5878]. To examine the structures of longer better-defined PHF segments, crystals of the MN423 Fab fragment were grown in the presence of two synthetic peptides derived from the PHF core C-terminus. For each, X-ray diffraction data were collected at 100 K at a synchrotron source and initial phases were obtained by molecular replacement.
authors.affiliation: Institute of Neuroimmunology, Slovak Academy of Sciences, Dubravska cesta 9, 84 510 Bratislava, Slovakia.
authors.forename: Rostislav
authors.initials: R
authors.lastname: Skrabana
authors.forename: Ondrej
authors.initials: O
authors.lastname: Cehlar
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Flachbartova
authors.forename: Andrej
authors.initials: A
authors.lastname: Kovac
authors.forename: Jozef
authors.initials: J
authors.lastname: Sevcik
authors.forename: Michal
authors.initials: M
authors.lastname: Novak
mesh_terms: D000544:Alzheimer Disease / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D003460:Crystallization; D018360:Crystallography, X-Ray; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology; D017433:Protein Structure, Secondary; D016875:tau Proteins / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D010446:Peptide Fragments; D016875:tau Proteins