title: Heat treatment eliminates 'Candidatus Liberibacter asiaticus' from infected citrus trees under controlled conditions.
abstract: Huanglongbing (HLB) is one of the most destructive diseases of citrus worldwide. The three known causal agents of HLB are species of α-proteobacteria: 'Candidatus Liberibacter asiaticus', 'Ca. L. africanus', and 'Ca. L. americanus'. Previous studies have found distinct variations in temperature sensitivity and tolerance among these species. Here, we describe the use of controlled heat treatments to cure HLB caused by 'Ca. L. asiaticus', the most prevalent and heat-tolerant species. Using temperature-controlled growth chambers, we evaluated the time duration and temperature required to suppress or eliminate the 'Ca. L. asiaticus' bacterium in citrus, using various temperature treatments for time periods ranging from 2 days to 4 months. Results of quantitative polymerase chain reaction (qPCR) after treatment illustrate significant decreases in the 'Ca. L. asiaticus' bacterial titer, combined with healthy vigorous growth by all surviving trees. Repeated qPCR testing confirmed that previously infected, heat-treated plants showed no detectable levels of 'Ca. L. asiaticus', while untreated control plants remained highly infected. Continuous thermal exposure to 40 to 42°C for a minimum of 48 h was sufficient to significantly reduce titer or eliminate 'Ca. L. asiaticus' bacteria entirely in HLB-affected citrus seedlings. This method may be useful for the control of 'Ca. Liberibacter'-infected plants in nursery and greenhouse settings.
authors.affiliation: United States Department of Agriculture, Agriculture Reserch Service, United States Horticultural Research Laboratory, Fort Pierce, FL 34945, USA.
authors.forename: Michele T
authors.initials: MT
authors.lastname: Hoffman
authors.forename: Melissa S
authors.initials: MS
authors.lastname: Doud
authors.forename: Lisa
authors.initials: L
authors.lastname: Williams
authors.forename: Mu-Qing
authors.initials: MQ
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Ding
authors.forename: Ed
authors.initials: E
authors.lastname: Stover
authors.forename: David
authors.initials: D
authors.lastname: Hall
authors.forename: Shouan
authors.initials: S
authors.lastname: Zhang
authors.forename: Lisa
authors.initials: L
authors.lastname: Jones
authors.forename: Mark
authors.initials: M
authors.lastname: Gooch
authors.forename: Laura
authors.initials: L
authors.lastname: Fleites
authors.forename: Wayne
authors.initials: W
authors.lastname: Dixon
authors.forename: Dean
authors.initials: D
authors.lastname: Gabriel
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Duan
mesh_terms: D030008:Catharanthus / Q000254:growth & development / Q000382:microbiology*; D002957:Citrus / Q000254:growth & development / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004780:Environment, Controlled; D005240:Feasibility Studies; D006358:Hot Temperature; D010641:Phenotype; D010935:Plant Diseases / Q000382:microbiology / Q000628:therapy*; D018515:Plant Leaves / Q000254:growth & development / Q000382:microbiology; D060888:Real-Time Polymerase Chain Reaction; D012230:Rhizobiaceae / Q000235:genetics / Q000502:physiology*; D014197:Trees; D014934:Wood
chemical_list: D004269:DNA, Bacterial