title: A switchable two-photon membrane tracer capable of imaging membrane-associated protein tyrosine phosphatase activities.
abstract: When I look into your cells: a two-photon dye (Flu7) was developed, which strongly fluoresces only upon selective binding to the plasma membrane of mammalian cells. Upon addition of a UV- and phosphatase-controlled quencher (Q12), the system exhibits ON/OFF/ON fluorescence switching and can be used to image membrane-associated receptor-like protein tyrosine phosphatase (RPTP) activity.
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Shen
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Xu
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D004330:Drosophila; D005260:Female; D008856:Microscopy, Fluorescence; D010766:Phosphorylation; D055668:Photochemical Processes; D017027:Protein Tyrosine Phosphatases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D017027:Protein Tyrosine Phosphatases