title: Protective effect of extract from Rumex aquaticus herba on ethanol-induced gastric damage in rats.
abstract: OBJECTIVE
  In this study, we investigated the gastroprotective effect of extract including quercetin-3-O-β-D-glucuronopyranoside (EIQ) from Rumex aquaticus herba against the ethanol-induced gastric damage in rats.
  
  
  METHODS
  The rats were divided into eight groups composed of a non-ethanol group, only EIQ (10 mg/kg) group, groups with absolute ethanol after pretreatment with various doses of EIQ (1, 3 and 10 mg/kg), rebamipide (10 mg/kg), stillen (40 mg/kg) and a control receiving only absolute ethanol. Ethanol-induced gastric lesions, lipid peroxidation, neutrophil infiltration and glutathione level were measured. Superoxide dismutase (SOD) and catalase activity were assessed by an assay kit. Protein expression of SOD, catalase or hemoxygenase-1 (HO-1) was assessed by western blotting analysis.
  
  
  CONCLUSIONS
  In the absolute ethanol treated group, gastric lesion and malondialdehyde levels were significantly increased with enhanced myeloperoxidase activity. Administration of EIQ 1 h prior to ethanol treatment significantly inhibited the formation of gastric lesions and the elevation of the malondialdehyde levels with myeloperoxidase activity. In addition, pretreatment with EIQ significantly increased the level of glutathione, and elevated the activity and protein expression of radical scavenging enzymes, such as SOD, catalase and HO-1. EIQ may exert anti-inflammatory and anti-oxidative effects against ethanol-induced gastric injury through the reduction of lipid peroxidation, myeloperoxidase activity and free radicals.
authors.affiliation: Department of Pharmacology, College of Pharmacy, Chung-Ang University, Seoul, South Korea.
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kwak
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Park
authors.forename: Thao Thanh
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Chung Hyo
authors.initials: CH
authors.lastname: Kim
authors.forename: Jong Mi
authors.initials: JM
authors.lastname: Lee
authors.forename: Jung Sook
authors.initials: JS
authors.lastname: Suh
authors.forename: Wan Kyunn
authors.initials: WK
authors.lastname: Whang
authors.forename: Uy Dong
authors.initials: UD
authors.lastname: Sohn
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D000431:Ethanol; D005978:Glutathione / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D031804:Rumex; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D017392:Thiobarbituric Acid Reactive Substances; C443401:quercetin 3-O-glucuronide; D000431:Ethanol; D011794:Quercetin; D002374:Catalase; D009195:Peroxidase; D006419:Heme Oxygenase (Decyclizing); D013482:Superoxide Dismutase; D005978:Glutathione