title: Ginger extract and zingerone ameliorated trinitrobenzene sulphonic acid-induced colitis in mice via modulation of nuclear factor-κB activity and interleukin-1β signalling pathway.
abstract: Ginger is a commonly used spice with anti-inflammatory potential. Colitis is the common pathological lesion of inflammatory bowel diseases. In this study, we investigated the therapeutic effects of ginger and its component zingerone in mice with 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Ginger and zingerone ameliorated TNBS-induced colonic injury in a dose-dependent manner. Pathway analysis of ginger- and zingerone-regulated gene expression profiles showed that ginger and zingerone significantly regulated cytokine-related pathways. Network analysis showed that nuclear factor-κB (NF-κB) and interleukin-1β (IL-1β) were key molecules involved in the expression of ginger- and zingerone-affected genes. Ex vivo imaging and immunohistochemical staining further verified that ginger and zingerone suppressed TNBS-induced NF-κB activation and IL-1β protein level in the colon. In conclusion, ginger improved TNBS-induced colitis via modulation of NF-κB activity and IL-1β signalling pathway. Moreover, zingerone might be the active component of ginger responsible for the amelioration of colitis induced by TNBS.
authors.affiliation: Department of Microbiology, China Medical University, Taichung, Taiwan. cyhsiang@mail.cmu.edu.tw
authors.forename: Chien-Yun
authors.initials: CY
authors.lastname: Hsiang
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Lo
authors.forename: Hui-Chi
authors.initials: HC
authors.lastname: Huang
authors.forename: Chia-Cheng
authors.initials: CC
authors.lastname: Li
authors.forename: Shih-Lu
authors.initials: SL
authors.lastname: Wu
authors.forename: Tin-Yun
authors.initials: TY
authors.lastname: Ho
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D005260:Female; D020939:Zingiber officinale / Q000737:chemistry*; D006139:Guaiacol / Q000008:administration & dosage / Q000031:analogs & derivatives*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage*; D015398:Signal Transduction / Q000187:drug effects; D014302:Trinitrobenzenesulfonic Acid / Q000009:adverse effects
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D010936:Plant Extracts; C013738:zingerone; D006139:Guaiacol; D014302:Trinitrobenzenesulfonic Acid