title: A two-photon fluorescent probe for intracellular detection of tyrosinase activity.
abstract: AAN effective sensor: The two-photon turn-on fluorescent probe NHU was synthesized to optically detect tyrosinase activity in vitro and in melanoma cells. NHU is composed of a 4-aminophenol moiety and a naphthylamine unit, both of which are connected through a urea linkage. Upon exposure to tyrosinase, the 4-aminophenol site is gradually oxidized to the corresponding orthoquinone, ultimately releasing the highly fluorescent product 6-acyl-N-methyl-2-naphthylamine (AAN).
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan, 430072, PR China.
authors.forename: Shengyong
authors.initials: S
authors.lastname: Yan
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.forename: Changcheng
authors.initials: C
authors.lastname: Wang
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
authors.forename: Boshi
authors.initials: B
authors.lastname: Fu
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Weng
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D015081:2-Naphthylamine / Q000737:chemistry / Q000378:metabolism; D000627:Aminophenols / Q000737:chemistry / Q000378:metabolism*; D045744:Cell Line, Tumor; D004789:Enzyme Activation; D057075:Enzyme Assays / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008545:Melanoma / Q000201:enzymology; D018613:Microscopy, Confocal / Q000379:methods; D014442:Monophenol Monooxygenase / Q000032:analysis / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D000627:Aminophenols; D005456:Fluorescent Dyes; D015081:2-Naphthylamine; D014442:Monophenol Monooxygenase; C026729:4-aminophenol