title: Isolation and characterization of canine amniotic membrane-derived multipotent stem cells.
abstract: Recent studies have shown that amniotic membrane tissue is a rich source of stem cells in humans. In clinical applications, the amniotic membrane tissue had therapeutic effects on wound healing and corneal surface reconstruction. Here, we successfully isolated and identified multipotent stem cells (MSCs) from canine amniotic membrane tissue. We cultured the canine amniotic membrane-derived multipotent stem cells (cAM-MSCs) in low glucose DMEM medium. cAM-MSCs have a fibroblast-like shape and adhere to tissue culture plastic. We characterized the immunophenotype of cAM-MSCs by flow cytometry and measured cell proliferation by the cumulative population doubling level (CPDL). We performed differentiation studies for the detection of trilineage multipotent ability, under the appropriate culture conditions. Taken together, our results show that cAM-MSCs could be a rich source of stem cells in dogs. Furthermore, cAM-MSCs may be useful as a cell therapy application for veterinary regenerative medicine.
authors.affiliation: Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sang-Bum
authors.initials: SB
authors.lastname: Park
authors.forename: Min-Soo
authors.initials: MS
authors.lastname: Seo
authors.forename: Hyung-Sik
authors.initials: HS
authors.lastname: Kim
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Kang
mesh_terms: D050156:Adipogenesis; D000650:Amnion / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D020219:Chondrogenesis; D004285:Dogs; D005434:Flow Cytometry / Q000379:methods; D020869:Gene Expression Profiling; D016130:Immunophenotyping; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D055495:Neurogenesis; D061251:Primary Cell Culture / Q000379:methods
chemical_list: D015415:Biomarkers