title: A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge.
abstract: A propargyl containing guanosine phosphoramidite was synthesized and incorporated into siRNA, enabling click-ligation with an azido fluorophore onto the nucleobase sugar edge. Duplex stability was not affected by labeling at this new site, which allowed deconvolution of the effects of label, structure and attachment site on RNAi activity.
authors.affiliation: Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, 5 Staudinger Weg, Mainz, D-55128, Germany.
authors.forename: Salifu
authors.initials: S
authors.lastname: Seidu-Larry
authors.forename: Bettina
authors.initials: B
authors.lastname: Krieg
authors.forename: Markus
authors.initials: M
authors.lastname: Hirsch
authors.forename: Mark
authors.initials: M
authors.lastname: Helm
authors.forename: Olwen
authors.initials: O
authors.lastname: Domingo
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D057930:Click Chemistry; D006151:Guanosine / Q000138:chemical synthesis / Q000737:chemistry*; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000737:chemistry*
chemical_list: D002241:Carbohydrates; D009943:Organophosphorus Compounds; D034741:RNA, Small Interfering; C434331:phosphoramidite; D006151:Guanosine; D012313:RNA