title: Crystallization and preliminary diffraction studies of GIM-1, a class B carbapenem-hydrolyzing β-lactamase.
abstract: GIM-1 is a member of the class B carbapenemases (metallo-β-lactamases; MBLs) and has a wide spectrum of activity against carbapenems, penicillins and extended-spectrum cephalosporins, but not aztreonam. GIM-1 presents an enormous challenge to infection control, particularly in the eradication of Gram-negative pathogens including Enterobacteriaceae, Pseudomonas aeruginosa, Acinetobacter baumannii and nonfermenters. There are presently few or no drugs in late-stage development for these pathogens and GIM-1 is a potential target for the development of antimicrobial agents against pathogens producing MBLs. In this study, GIM-1 was cloned, overexpressed and crystallized. The GIM-1 crystals diffracted to 1.4 Å resolution and belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 38.5, b = 67.6, c = 72.8 Å. One molecule is present in the asymmetric unit, with a corresponding V(M) of 1.69 Å(3) Da(-1) and a solvent content of 27.1%.
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Myoung-Ki
authors.initials: MK
authors.lastname: Hong
authors.forename: Jung Hun
authors.initials: JH
authors.lastname: Lee
authors.forename: Dae Beom
authors.initials: DB
authors.lastname: Kwon
authors.forename: Jin-Kwang
authors.initials: JK
authors.lastname: Kim
authors.forename: Thi-Huyen
authors.initials: TH
authors.lastname: Tran
authors.forename: Dinh-Duc
authors.initials: DD
authors.lastname: Nguyen
authors.forename: Byeong Chul
authors.initials: BC
authors.lastname: Jeong
authors.forename: Sang Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D003460:Crystallization; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D001618:beta-Lactamases / Q000737:chemistry*
chemical_list: D001618:beta-Lactamases; C495217:beta-lactamase GIM-1, Pseudomonas aeruginosa