title: Structure and properties of maize starch processed with a combination of α-amylase and pullulanase.
abstract: The dissolution and digestion characteristics of maize starch processed with a combination of α-amylase and pullulanase (ERS) were investigated. The results were compared with those of high pressure-processed RS (HRS) and regular maize starch. The ERS exhibited a considerably lower dissolution and digestibility than the HRS under conditions that simulated the stomach and small intestine in vitro. Infrared spectroscopy showed two bands at 1047 and 1002 cm(-1) for ERS, which suggested an easily produced RS during the retrogradation process after enzymatic treatment. High-performance size-exclusion chromatography showed a hydrolyzed ERS forming a critical molecular weight fraction (MW 4833 Da; DP 29.8). X-ray diffraction analysis also revealed a significant difference between the types of HRS (B+V type) and ERS (in the transformation from B+V to V type) crystallinities, and the crystal structure change leads to improved anti-enzymatic properties of ERS. These results indicate that the enzyme-combined method is a more promising technique than high pressure to prepare RS from maize starch with greater resistance.
authors.affiliation: The State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. hxinzh@hotmail.com
authors.forename: Huanxin
authors.initials: H
authors.lastname: Zhang
authors.forename: Yaoqi
authors.initials: Y
authors.lastname: Tian
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Bai
authors.forename: Xueming
authors.initials: X
authors.lastname: Xu
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Jin
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D006026:Glycoside Hydrolases / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D013552:Swine; D003313:Zea mays / Q000737:chemistry*; D000516:alpha-Amylases / Q000737:chemistry*
chemical_list: D013213:Starch; D006026:Glycoside Hydrolases; D000516:alpha-Amylases; C100181:pullulanase