title: Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.
abstract: We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
authors.affiliation: Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.
authors.forename: V M
authors.initials: VM
authors.lastname: Corman
authors.forename: I
authors.initials: I
authors.lastname: Eckerle
authors.forename: T
authors.initials: T
authors.lastname: Bleicker
authors.forename: A
authors.initials: A
authors.lastname: Zaki
authors.forename: O
authors.initials: O
authors.lastname: Landt
authors.forename: M
authors.initials: M
authors.lastname: Eschbach-Bludau
authors.forename: S
authors.initials: S
authors.lastname: van Boheemen
authors.forename: R
authors.initials: R
authors.lastname: Gopal
authors.forename: M
authors.initials: M
authors.lastname: Ballhause
authors.forename: T M
authors.initials: TM
authors.lastname: Bestebroer
authors.forename: D
authors.initials: D
authors.lastname: Muth
authors.forename: M A
authors.initials: MA
authors.lastname: Müller
authors.forename: J F
authors.initials: JF
authors.lastname: Drexler
authors.forename: M
authors.initials: M
authors.lastname: Zambon
authors.forename: A D
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: R M
authors.initials: RM
authors.lastname: Fouchier
authors.forename: C
authors.initials: C
authors.lastname: Drosten
mesh_terms: D028941:Coronavirus 229E, Human / Q000235:genetics / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000175:diagnosis / Q000235:genetics / Q000821:virology*; D058957:Coronavirus NL63, Human / Q000235:genetics / Q000302:isolation & purification; D028962:Coronavirus OC43, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016366:Open Reading Frames; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics* / Q000302:isolation & purification*; D012529:Saudi Arabia; D012680:Sensitivity and Specificity; D014195:Travel; D014759:Viral Envelope Proteins; D000086082:Viroporin Proteins
chemical_list: C501689:E protein, SARS coronavirus; D014759:Viral Envelope Proteins; D000086082:Viroporin Proteins