title: Bidirectional Notch activation represses fusion competence in swarming adult Drosophila myoblasts.
abstract: A major aspect of indirect flight muscle formation during adult Drosophila myogenesis involves transition of a semi-differentiated and proliferating pool of myoblasts to a mature myoblast population, capable of fusing with nascent myotubes and generating mature muscle fibers. Here we examine the molecular genetic programs underlying these two phases of myoblast differentiation. We show that the cell adhesion proteins Dumbfounded (Duf) and Sticks and stones (Sns), together with their paralogs Roughest (Rst) and Hibris (Hbs), respectively, are required for adhesion of migrating myoblasts to myotubes and initiation of myoblast-myotube fusion. As myoblasts approach their myotube targets, they are maintained in a semi-differentiated state by continuous Notch activation, where each myoblast provides the ligand Delta to its neighbors. This unique form of bidirectional Notch activation is achieved by finely tuning the levels of the ligand and receptor. Activation of Notch signaling in myoblasts represses expression of key fusion elements such as Sns. Only upon reaching the vicinity of the myotubes does Notch signaling decay, leading to terminal differentiation of the myoblasts. The ensuing induction of proteins required for fusion enables myoblasts to fuse with the myotubes and give rise to subsequent muscle fiber growth.
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100,  Israel.
authors.forename: Boaz
authors.initials: B
authors.lastname: Gildor
authors.forename: Eyal D
authors.initials: ED
authors.lastname: Schejter
authors.forename: Ben-Zion
authors.initials: BZ
authors.lastname: Shilo
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism; D004330:Drosophila / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism*; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; D005136:Eye Proteins; D007136:Immunoglobulins; D008565:Membrane Proteins; D009124:Muscle Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; C410904:SNS protein, Drosophila; C442230:hbs protein, Drosophila; C413082:kirre protein, Drosophila; C085209:rst protein, Drosophila