title: Identification of the transcript isoforms and expression characteristics for chicken Lpin1.
abstract: Lpin1 was a gene with important effects on controlling lipid/energy metabolism in humans and mice. However, little was known about chicken Lpin1 gene. In the present study, two transcript isoforms of chicken Lpin1 were identified. Lpin1-α was predicted encoding one 902 amino acid protein, whereas Lpin1-δ was predicted encoding one 918 amino acid protein with an insertion of 48-bp fragment from intron 12 of chicken Lpin1-α, and a conservative element was found to be located in intron 12 of chicken Lpin1-α genomic sequence. Ten variants were identified from chicken Lpin1-α coding sequence, and two missense mutations were predicted to affect the protein function of Lpin1. Reverse transcription PCR (RT-PCR) analysis revealed that chicken total Lpin1, Lpin1-α and Lpin1-δ were expressed in all analyzed tissues, and presented clear tissue expression differences. Real-time quantitative RT-PCR revealed that 30% energy restriction significantly elevated the total Lpin1 mRNA expression level in hepatic (P < 0.01) and adipose (P < 0.01) tissues of birds. Chicken total Lpin1 gene mRNA expression level presented a significantly inverse correlation with some traits including abdominal fat rate (P < 0.01), serum high-density lipoprotein (P < 0.05) and total cholesterol (P < 0.05), which would make a foundation for the further study on chicken Lpin1 gene function.
authors.affiliation: College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University, No. 95 Wenhua Road, Zhengzhou, Henan 450002, PR China.
authors.forename: X K
authors.initials: XK
authors.lastname: Wang
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Huang
authors.forename: X T
authors.initials: XT
authors.lastname: Kang
authors.forename: J P
authors.initials: JP
authors.lastname: Wang
authors.forename: G X
authors.initials: GX
authors.lastname: Li
authors.forename: R R
authors.initials: RR
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D002149:Energy Intake; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames; D009928:Organ Specificity; D010711:Phosphatidate Phosphatase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D009687:Nuclear Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D010711:Phosphatidate Phosphatase