title: Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling.
abstract: Sequencing-based approaches have led to new insights about DNA methylation. While many different techniques for genome-scale mapping of DNA methylation have been employed, throughput has been a key limitation for most. To further facilitate the mapping of DNA methylation, we describe a protocol for gel-free multiplexed reduced representation bisulfite sequencing (mRRBS) that reduces the workload dramatically and enables processing of 96 or more samples per week. mRRBS achieves similar CpG coverage to the original RRBS protocol, while the higher throughput and lower cost make it better suited for large-scale DNA methylation mapping studies, including cohorts of cancer samples.
authors.forename: Patrick
authors.initials: P
authors.lastname: Boyle
authors.forename: Kendell
authors.initials: K
authors.lastname: Clement
authors.forename: Hongcang
authors.initials: H
authors.lastname: Gu
authors.forename: Zachary D
authors.initials: ZD
authors.lastname: Smith
authors.forename: Michael
authors.initials: M
authors.lastname: Ziller
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Fostel
authors.forename: Laurie
authors.initials: L
authors.lastname: Holmes
authors.forename: Jim
authors.initials: J
authors.lastname: Meldrim
authors.forename: Fontina
authors.initials: F
authors.lastname: Kelley
authors.forename: Andreas
authors.initials: A
authors.lastname: Gnirke
authors.forename: Alexander
authors.initials: A
authors.lastname: Meissner
mesh_terms: D000818:Animals; D018899:CpG Islands; D019175:DNA Methylation; D016678:Genome; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D017422:Sequence Analysis, DNA / Q000191:economics / Q000379:methods*; D013447:Sulfites / Q000494:pharmacology
chemical_list: D013447:Sulfites; C042345:hydrogen sulfite