title: Equilibrium self-association of tropomyosin.
abstract: It has recently been reported that tropomyosin exists exclusively as a dimer in physiological salt conditions. It is shown in the present work using analytical ultracentrifugation that, on the contrary, tropomyosin is in equilibrium between monomer, dimer and tetramer with a weak tendency to dimerize and tetramerize. Such a finding has consequences for the assembly of the tropomyosin-actin complex.
authors.affiliation: Boston Biomedical Research Institute, Watertown, MA 02472, United States.
authors.forename: Walter F
authors.initials: WF
authors.lastname: Stafford
authors.forename: Eunhee
authors.initials: E
authors.lastname: Lee
authors.forename: Philip
authors.initials: P
authors.lastname: Graceffa
mesh_terms: D000818:Animals; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D058105:Polymerization; D011485:Protein Binding; D011817:Rabbits; D012996:Solutions; D013696:Temperature; D013816:Thermodynamics; D014335:Tropomyosin / Q000737:chemistry*; D014461:Ultracentrifugation
chemical_list: D012996:Solutions; D014335:Tropomyosin