title: Characterization and biological activity of Solidago canadensis complex.
abstract: Polyphenolic-polysaccharide-protein complex has been isolated from flowers of Solidago canadensis L. by hot alkaline extraction procedure. Compositional analyses of S canadensis complex revealed the presence of carbohydrates (43 wt%), protein (27 wt%), phenolics (12 wt%), uronic acids (10 wt%) and inorganic material (8 wt%). The carbohydrate part was rich in neutral sugars (81 wt%) while uronids were determined in lower amount (19 wt%). Monosaccharide analysis of carbohydrate part revealed the presence of five main sugar components, i.e. rhamnose (~23 wt%), arabinose (~20 wt%), uronic acids (~19 wt%), galactose (~17 wt%) and glucose (~14 wt%), and indicated thus the presence of rhamnogalacturonan and arabinogalactan in S. canadensis complex. HPLC analysis of complex showed one single peak of molecule mass at 11.2 kDa. Antitussive activity tests, performed in three doses of Solidago complex, showed the reduction of the number of cough efforts in the dose-dependent manner. Higher doses (50 and 75 mg/kg b.w.) were shown to be by 15 and 20% more effective than that of lower one (25mg/kg b.w.). However, the antitussive effect of the highest dose (75 mg/kg b.w.) was by 10% lower in comparison with that of codeine, the strongest antitussive agent. Besides, the highest dose of the complex (75 mg/kg b.w.) significantly decreased values of specific airways resistance and their effect remained longer as that of salbutamol, a representative of classic antiasthmatic drugs.
authors.affiliation: Department of Pharmacology, Jessenius Faculty of Medicine, Comenius University, SK-03753, Martin, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Šutovská
authors.forename: P
authors.initials: P
authors.lastname: Capek
authors.forename: M
authors.initials: M
authors.lastname: Kocmálová
authors.forename: S
authors.initials: S
authors.lastname: Fraňová
authors.forename: I
authors.initials: I
authors.lastname: Pawlaczyk
authors.forename: R
authors.initials: R
authors.lastname: Gancarz
mesh_terms: D000403:Airway Resistance / Q000187:drug effects*; D000420:Albuterol / Q000737:chemistry; D000818:Animals; D000996:Antitussive Agents / Q000737:chemistry / Q000494:pharmacology; D001993:Bronchodilator Agents / Q000737:chemistry / Q000494:pharmacology*; D003061:Codeine / Q000737:chemistry / Q000494:pharmacology; D003371:Cough / Q000188:drug therapy*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D036624:Solidago / Q000737:chemistry*
chemical_list: D000996:Antitussive Agents; D001993:Bronchodilator Agents; D010936:Plant Extracts; D000420:Albuterol; D003061:Codeine