Human carotid body HIF and NGB expression during human development and aging.
Hypoxia inducible factor 1(HIF-1α) is the regulator of oxygen homeostasis in tissue correlated with neuroglobin (NGB) a member of the family of globins in vertebrates. The present study investigates, the expression and the location of NGB, HIF-1α in human carotid bodies, sampled at autopsy from children (mean age: 2 year ±), young (mean age: 27.5) and 4 old subjects (mean age: 73.5). The percentage of NGB positive area was higher in the old subjects (4.4 ±2.8%), as compared with the young ones (2.4 ±1.8%) and children (1.0 ±1.8%). Positive HIF-1α nuclei were detected in young and old subjects (1.0 ±0.14% vs 3.0 ±0.28%, respectively), whereas CB tissues from children did not show any HIF-1α reaction. The increase of NGB and HIF-1α expression suggests a possible role of the two oxygen sensors in the aging processes. Even though the physiological role of NGB is not well understood, it could be suggested that is act as a respiratory protein connected with HIF.
Department of Neurosciences and Imaging, University of Chieti, Chieti, Italy. digiulio@unich.it
Camillo
C
Di Giulio
S
S
Zara
A
A
Cataldi
Andrea
A
Porzionato
Mieczyslaw
M
Pokorski
Raffaele
R
De Caro
D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D002344:Carotid Body / Q000502:physiology*; D002675:Child, Preschool; D005914:Globins / Q000032:analysis / Q000502:physiology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis / Q000502:physiology*; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000502:physiology*; D000078787:Neuroglobin
C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D005914:Globins