Zinc induced phytotoxicity mechanism involved in root growth of Triticum aestivum L.
This study investigated the inhibition mechanism of root growth in wheat seedlings when exposed to different zinc (Zn) concentrations. All applied Zn concentration did not affect seed germination, but reduced root length; in contrast, only Zn at 3mM inhibited significantly the growth of shoot. The loss of cell viability and the significant increases of lignification as well as the increases of hydrogen peroxide (H(2)O(2)), superoxide radical (O(2)(-)) and malondialdehyde levels were observed in the root tissue exposed to Zn treatment. And also, Zn stress led to the inhibition of cell-wall bound peroxidase. Moreover, NADPH oxidase inhibitor diphenylene iodonium could block greatly the elevation of O(2)(-) generation in Zn-treated roots. Therefore, the increased H(2)O(2) generation was dependent on the extracellular O(2)(-) production derived from plasma membrane NADPH oxidase. In addition, the loss of cell viability and the significant increases of lignification in response to the highest Zn concentration may be associated with the remarkable reduction of root growth in wheat seedlings.
School of Life Science, Northwest Normal University, Lanzhou 730070, PR China.
Xiaoning
X
Li
Yingli
Y
Yang
Jin
J
Zhang
Linyun
L
Jia
Qiaoxia
Q
Li
Tengguo
T
Zhang
Kexiong
K
Qiao
Shengchun
S
Ma
D002470:Cell Survival / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D009861:Onium Compounds / Q000494:pharmacology; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000254:growth & development; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D013481:Superoxides / Q000378:metabolism; D014908:Triticum / Q000187:drug effects* / Q000254:growth & development; D015032:Zinc / Q000633:toxicity*
D004791:Enzyme Inhibitors; D009861:Onium Compounds; D013481:Superoxides; D008315:Malondialdehyde; C007517:diphenyleneiodonium; D006861:Hydrogen Peroxide; D010544:Peroxidases; D019255:NADPH Oxidases; D015032:Zinc