Protective effect of curcumin against formaldehyde-induced genotoxicity in A549 Cell Lines.
Formaldehyde is ubiquitous in the environment. It is known to be a genotoxic substance. We hypothesized that reactive oxygen species (ROS) and lipid peroxidation are involved in formaldehyde-induced genotoxicity in human lung cancer cell lines A549. To test this hypothesis, we investigated the effects of antioxidant on formaldehyde-induced genotoxicity in A549 Cell Lines. Formaldehyde exposure caused induction of DNA-protein cross-links (DPCs). Curcumin is an important antioxidant. Formaldehyde significantly increased malondialdehyde (MDA) levels, and decreased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity. In addition, the activation of NF-κB and AP-1 were induced by formaldehyde treatment. Pretreatment with curcumin counteracted formaldehyde-induced oxidative stress, ameliorated DPCs and attenuated activation of NF-κB and AP-1 in A549 Cell Lines. These results, taken together, suggest that formaldehyde induced genotoxicity through its ROS and lipid peroxidase activity and caused DPCs effects in A549 cells.
School of Public Health, Medical College, Wuhan University of Science and Technology, Wuhan, 430081, Hubei, China.
Ben-Yan
BY
Zhang
Yu-Qin
YQ
Shi
Xin
X
Chen
Juan
J
Dai
Zhong-Fa
ZF
Jiang
Ning
N
Li
Zhi-Bing
ZB
Zhang
D000975:Antioxidants / Q000494:pharmacology*; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D004249:DNA Damage; D005557:Formaldehyde / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects / Q000235:genetics; D008315:Malondialdehyde / Q000378:metabolism; D009153:Mutagens / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D009153:Mutagens; D017382:Reactive Oxygen Species; D005557:Formaldehyde; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D003474:Curcumin