A survey of 6,300 genomic fragments for cis-regulatory activity in the imaginal discs of Drosophila melanogaster.
Over 6,000 fragments from the genome of Drosophila melanogaster were analyzed for their ability to drive expression of GAL4 reporter genes in the third-instar larval imaginal discs. About 1,200 reporter genes drove expression in the eye, antenna, leg, wing, haltere, or genital imaginal discs. The patterns ranged from large regions to individual cells. About 75% of the active fragments drove expression in multiple discs; 20% were expressed in ventral, but not dorsal, discs (legs, genital, and antenna), whereas ∼23% were expressed in dorsal but not ventral discs (wing, haltere, and eye). Several patterns, for example, within the leg chordotonal organ, appeared a surprisingly large number of times. Unbiased searches for DNA sequence motifs suggest candidate transcription factors that may regulate enhancers with shared activities. Together, these expression patterns provide a valuable resource to the community and offer a broad overview of how transcriptional regulatory information is distributed in the Drosophila genome.
Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, 701 W. 168th Street, HHSC 1104, New York, NY 10032, USA.
Aurélie
A
Jory
Carlos
C
Estella
Matt W
MW
Giorgianni
Matthew
M
Slattery
Todd R
TR
Laverty
Gerald M
GM
Rubin
Richard S
RS
Mann
D000818:Animals; D030801:Animals, Genetically Modified; D057506:Arthropod Antennae / Q000378:metabolism; D054239:Blastodisc / Q000378:metabolism; D016208:Databases, Factual; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D005123:Eye / Q000378:metabolism; D017930:Genes, Reporter; D016678:Genome; D060227:Imaginal Discs / Q000378:metabolism*; D050436:Regulatory Elements, Transcriptional; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014921:Wings, Animal / Q000378:metabolism
D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D014157:Transcription Factors