Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats.
Male Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order to provide the characterization of the tannins present in the bark of R. mangle. Results showed that EtOAc fraction increased the levels of IL-10 (∗∗P < 0.01) and diminished the levels of TNF-α (∗∗∗P < 0.001) and IL-6 (∗∗P < 0.01). BuOH fraction reduced the MPO activity (∗∗P < 0.01) and levels of TBARS (∗∗∗P < 0.001); it also increased COX-1 expression, diminished the levels of TNF-α (∗∗∗P < 0.001), and increased the levels of IL-12 (∗∗∗P < 0.001). Besides, both treatments augmented the levels of GSH (∗P < 0.05), the activity of GSH-Px (∗∗P < 0.01 for BuOH fraction and ∗∗∗P < 0.001 for EtOAc fraction), and CAT (∗∗P < 0.01). In conclusion, both treatments ameliorated the injury induced by TNBS through different mechanisms, probably by their chemical composition which directed its activity into an antioxidant or anti-inflammatory response, leading to an immune modulation.
Departamento de Farmacologia, Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil.
Felipe Meira
FM
de Faria
Anderson
A
Luiz-Ferreira
Eduardo Augusto Rabelo
EA
Socca
Ana Cristina Alves
AC
de Almeida
Ricardo José
RJ
Dunder
Luis Paulo
LP
Manzo
Marcelo Aparecido
MA
da Silva
Wagner
W
Vilegas
Ariane Leite
AL
Rozza
Cláudia Helena
CH
Pellizzon
Lourdes Campaner
LC
Dos Santos
Alba Regina Monteiro
AR
Souza Brito