Molecularly imprinted nanoparticles with nontailing peaks in capillary electrochromatography.
The combination of microparticles of molecularly imprinted polymers (MIPs) with partial filling capillary electrochromatography (CEC) has previously been demonstrated for the enantiomer separation. In this paper, precipitation polymerization was used to prepare d-zopiclone imprinted nanoparticles (50-80 nm) by a strategy of the dilution of pre-polymerization mixtures. The influence of some important parameters on the preparation of MIPs nanoparticles, including template to monomer ratio, type and amount of cross-linking monomer, and functional monomer composition ratio were investigated. In addition, the effect of separation condition, e.g., organic modifier content, pH value and salt concentration of buffer, on the electrochromatographic behavior of the MIP nanoparticles were studied. In spite of lower selectivity factor (1.11), high column performance (theoretical plates 41,400) of template was obtained and the resolution of enantiomers separation was 4.75 under the optimized conditions. Compared to the previously reported MIP microparticles, the MIP nanoparticles showed good peak symmetry and an ability of high speed separation (<15 min) in CEC mode.
Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
Xiao
X
Liu
Ze-Hui
ZH
Wei
Yan-Ping
YP
Huang
Jin-Rong
JR
Yang
Zhao-Sheng
ZS
Liu
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D046529:Microscopy, Electron, Transmission; D054802:Molecular Imprinting; D053758:Nanoparticles