Mass spectrometric analysis of EPO IEF-PAGE interfering substances in nitrile examination gloves.
Direct detection of doping with recombinant erythropoietins (rhEPO) is accomplished by isoelectric focusing (IEF) or sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis (PAGE). In a recent publication, Lasne et al. (Electrophoresis 2011, 32, 1444) showed that improper use of nitrile examination gloves during sample collection, sample preparation, and IEF-PAGE may lead to distorted or absent EPO IEF-profiles. In order to clarify which substances are responsible for this observation, a mass spectrometric study on water extractable compounds found in nitrile gloves was performed. Several substance classes were shown to be present, among them polyethylene glycols (PEG), anionic and nonionic surfactants, as well as alcohol ethoxylates and plasticizers. It could be demonstrated that alkylbenzenesulfonates, the main category of detectable anionic detergents, and among them sodium dodecylbenzenesulfonate (SDBS) and its homologs, are the prime reason for the interference of nitrile gloves with EPO IEF-PAGE.
Doping Control Laboratory, AIT Seibersdorf Laboratories, A-2444, Seibersdorf, Austria.
Christian
C
Reichel
D001557:Benzenesulfonates / Q000032:analysis; D003902:Detergents / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004921:Erythropoietin / Q000032:analysis*; D017575:Gloves, Protective; D007525:Isoelectric Focusing / Q000379:methods; D013058:Mass Spectrometry; D009570:Nitriles / Q000737:chemistry; D011994:Recombinant Proteins / Q000032:analysis; D013048:Specimen Handling / Q000379:methods*
D001557:Benzenesulfonates; D003902:Detergents; D009570:Nitriles; D011994:Recombinant Proteins; D004921:Erythropoietin; C001114:dodecylbenzenesulfonic acid