Effect of carnosine on prooxidant-antioxidant balance in several tissues of rats exposed to chronic cold plus immobilization stress.
In this study, we investigated the effect of L-carnosine (CAR) on prooxidant-antioxidant balance in several tissues of rats exposed to chronic stress. Both cold and immobilization stresses were applied to rats at the same time. In the stress group, rats were placed in restraint cages and kept in a cold room (+4°C) for 1 h for 21 days (5 days a week). Rats were injected with CAR (250 mg/kg, i.p.) at 30 min before stress application. Malondialdehyde, diene conjugate, protein carbonyl and nitrotyrosine levels, nonenzymatic (glutathione, vitamin E, and vitamin C), and enzymatic (catalase, superoxide dismutase and glutathione peroxidase) antioxidants were determined in the liver, heart, and brain tissues. Chronic cold plus immobilization stress was observed to affect especially the prooxidant-antioxidant status in the brain tissue of rats. This is the first report showing the beneficial effects of CAR on oxidative stress in the brain in rats exposed to stress.
Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
Esra Betül
EB
Kalaz
Betül
B
Evran
Seval
S
Develi-İş
Pervin
P
Vural
Semra
S
Dogru-Abbasoglu
Müjdat
M
Uysal
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002336:Carnosine / Q000494:pharmacology*; D003080:Cold Temperature; D004797:Enzyme-Linked Immunosorbent Assay; D007103:Immobilization; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013312:Stress, Physiological
D000975:Antioxidants; D017382:Reactive Oxygen Species; D002336:Carnosine