Identification of type II toxin-antitoxin modules in Burkholderia pseudomallei.
Type II toxin-antitoxin (TA) systems are believed to be widely distributed amongst bacteria although their biological functions are not clear. We have identified eight candidate TA systems in the genome of the human pathogen Burkholderia pseudomallei. Five of these were located in genome islands. Of the candidate toxins, BPSL0175 (RelE1) or BPSS1060 (RelE2) caused growth to cease when expressed in Escherichia coli, whereas expression of BPSS0390 (HicA) or BPSS1584 (HipA) (in an E. coli ΔhipBA background) caused a reduction in the number of culturable bacteria. The cognate antitoxins could restore growth and culturability of cells.
Department of Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, UK.
Aaron
A
Butt
Claudia
C
Müller
Nicholas
N
Harmer
Richard W
RW
Titball
D000992:Antitoxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016957:Burkholderia pseudomallei / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D019295:Computational Biology; D003470:Culture Media; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D016680:Genome, Bacterial / Q000235:genetics; D006801:Humans
D000992:Antitoxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media