[Changes of microRNA expression profiles in Vero cells induced by HSV-2 LAT overexpression].
OBJECTIVE
To investigate the changes in the microRNA expression profile of Vero cells induced by HSV-2 LAT overexpression.


METHODS
The full-length open reading frame of HSV-2 LAT was synthesized and cloned into pRetroQ- AcGFP1-C1 vector, and the recombinant retrovirus expressing HSV-2 LAT was packaged. Using a microRNA microarray, the microRNA expression profile changes in Vero cells were analyzed after infection with the recombinant retrovirus.


RESULTS
In Vero cells infected with the recombinant retrovirus for stable HSV-2 LAT overexpression, 5 microRNAs (hsa-miR-23a*, kshv-miR-K12-3, hsa-miR-943, hsa-miR-634, and hsa-miR-1270) were up-regulated and 5 (hsa-miR-181a-2*, hsa-miR-450b-5p, hsa-miR-31, hsa-miR-24, and kshv-miR-K12-12*) were down-regulated.


CONCLUSIONS
The expression of HSV-2 LAT can induce changes in microRNA expression profile in Vero cells.
Department of Dermatology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, China. wangying301@yahoo.com.cn
Ying
Y
Wang
Jianyong
J
Fan
Huilan
H
Yang
Jianyun
J
Chen
D000818:Animals; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs; D020411:Oligonucleotide Array Sequence Analysis; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
C477254:LAT protein, Human herpesvirus 2; D035683:MicroRNAs; D014764:Viral Proteins