High-resolution homonuclear 2D NMR of carbon-13 enriched metabolites and their mixtures.
High-resolution 2D (13)C-(13)C NMR correlation spectra of uniformly (13)C-labeled molecules in solution are obtained by homonuclear (13)C-decoupling along both dimensions by the application of indirect covariance NMR to constant-time NMR spectra. The spectra are optimally suited for chemical structure elucidation and molecular identification of the components of complex mixtures, such as ones from uniformly (13)C-labeled cell cultures.
National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL 32310, USA.
Fengli
F
Zhang
Lei
L
Bruschweiler-Li
Rafael
R
Brüschweiler
D000465:Algorithms; D000596:Amino Acids / Q000032:analysis; D002247:Carbon Isotopes; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D007553:Isotope Labeling; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*
D000596:Amino Acids; D002247:Carbon Isotopes; D005947:Glucose