Evaluation of the anti-angiogenic activity of saponins from Maesa lanceolata by different assays.
Angiogenesis, in which a vascular network is established from pre-existing vessels, is a complex multistep process. Mechanisms underlying angiogenesis can be investigated using a variety of in vitro, ex vivo and in vivo approaches. Evaluation of several promising plants and plant metabolites, including terpenoids, revealed promising anti-angiogenic activity. Since the maesasaponins displayed anti-angiogenic activity in the chick chorioallantoic membrane (CAM) assay, their activity was further investigated in several test systems. The rat aorta ring assay was compared with the placental vein assay and then selected for the ex vivo investigation of the saponins. Besides their effect on the viability of HUVEC, the anti-angiogenic capacity of the compounds was also investigated in an in vivo zebrafish assay. The activity of the saponins in the viability assay was more pronounced than in the rat aorta ring assay and similar to the effect observed in the CAM assay. The use of different test systems, however, implies different results in the case of saponins.
Laboratory of Pharmacognosy and Pharmaceutical Analysis, Department of Pharmaceutical Sciences; University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium. Kenn.Foubert@ua.ac.be
Kenn
K
Foubert
Annelies
A
Breynaert
Mart
M
Theunis
Rita
R
Van Den Bossche
Guido R Y
GR
De Meyer
André
A
Van Daele
Ahmad
A
Faizal
Alain
A
Goossens
Danny
D
Geelen
Edward M
EM
Conway
Arnold
A
Vlietinck
Luc
L
Pieters
Sandra
S
Apers
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000502:physiology; D002642:Chick Embryo; D005260:Female; D006801:Humans; D010920:Placenta / Q000098:blood supply; D011247:Pregnancy; D029623:Primulaceae / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology*; D015027:Zebrafish
D020533:Angiogenesis Inhibitors; D012503:Saponins