Identification of novel interleukin 1 beta family genes in Japanese flounder Paralichthys olivaceus.
Gene in the interleukin 1 family plays a central role in the regulation of immune and inflammatory responses. Here, we describe two novel IL-1-like gene in Japanese flounder Paralichthys olivaceus (jfIL1-L1 and jfIL1-L2). jfIL1β-L1 was homologous to Nile Tilapia IL-1β-like gene and Arctic char IL-1, and jfIL1β-L2 showed homology to hypothetical protein LOC100699119 of Nile Tilapia and rainbow trout Oncorhynchus mykiss IL-1 receptor agonist (RA). The deduced amino acids sequences of these IL-1β-like genes showed very low identities to the Japanese flounder IL-1 (jfIL-1). Phylogenetic analysis confirmed that jfIL1β-L1 and -L2 were distinct from jfIL-1. The gene encoding the predicted ORF of jfIL1β-L1 and -L2 is divided into 6 exons and 7 exons, respectively. Transcripts of jfIL1β-L1 were detected in gills, intestine, kidney and spleen, and those of jfIL1β-L2 were detected in gills, intestine and spleen. The mRNA levels of jfIL1β-L1 and -L2 were not effect or slightly decreased by treatment with LPS and the formalin-killed cells of Edwardsiella tarda whilst mRNA levels of jfIL1β were significantly increased in the kidney and spleen at 6 h by these treatments.
Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan.
Apichaya
A
Taechavasonyoo
Hidehiro
H
Kondo
Reiko
R
Nozaki
Yutaka
Y
Suzuki
Ikuo
I
Hirono
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D020609:Edwardsiella tarda / Q000276:immunology; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005412:Flatfishes / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation; D053583:Interleukin-1beta / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017385:Sequence Homology
D018076:DNA, Complementary; D029941:Fish Proteins; D053583:Interleukin-1beta; D012333:RNA, Messenger