Water soluble fluorescent-magnetic perylenediimide-containing maghemite-nanoparticles for bimodal MRI/OI imaging.
The protein shell of apoferritin-encapsulated maghemite nanoparticles was functionalized with two different red-emitting perylenediimide fluorophores (PDI). One glycosacharide-PDI complex has been synthesized for the first time to be labeled to apoferritin-encapsulated maghemite nanoparticles. Bifunctionality of maghemite@perylenediimide was demonstrated by both magnetic-core and fluorescent-labeled shell properties. SQUID measurements confirmed superparamagnetic behavior above 35K. Fluorescence of perylenediimides is retained once attached to the magnetic nanoparticle. Moreover, fluorescence microscopy showed that one of these fluorescent-magnetic nanoparticles was specifically internalized in bifidobacteria without affecting cell viability. These results revealed that the dual-modal imaging probes of maghemite@perylenediimide nanoparticles have the potential to be used as optical/MR dual imaging agents.
Departamento de Química Inorgánica, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain.
Natividad
N
Gálvez
Ewelina J
EJ
Kedracka
Fernando
F
Carmona
F Javier
FJ
Céspedes-Guirao
Enrique
E
Font-Sanchis
Fernando
F
Fernández-Lázaro
Ángela
Á
Sastre-Santos
José M
JM
Domínguez-Vera
D001052:Apoferritins / Q000737:chemistry; D001644:Bifidobacterium / Q000254:growth & development; D003287:Contrast Media / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007094:Imides / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D053768:Metal Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging; D010316:Particle Size; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D014867:Water / Q000737:chemistry
D003287:Contrast Media; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D007094:Imides; C521332:perylenediimide; D014867:Water; C000499:ferric oxide; D010569:Perylene; D001052:Apoferritins