Quantitative Glycomics of Cultured Cells Using Isotopic Detection of Aminosugars with Glutamine (IDAWG).
IDAWG (Isotopic Detection of Aminosugars With Glutamine) is a newly reported, in vivo, stable isotopic labeling strategy for quantitative glycomics of cultured cells. Detailed procedures are provided for glycan analysis using IDAWG including labeling, release of both N- and O-linked glycans, permethylation, and mass spectrometry analysis. The methods for data processing and calculations are also introduced here but have not yet been automated.
Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia.
Meng
M
Fang
Jae-Min
JM
Lim
Lance
L
Wells