MicroRNAs and zinc metabolism-related gene expression in prostate cancer cell lines treated with zinc(II) ions.
MicroRNAs (miRNAs) are a large class of single-stranded RNA molecules involved             in post-transcriptional gene silencing. miRNAs not only regulate various developmental             and physiological processes but also are involved in cancer development. Additionally,             they can be considered as biomarkers of some pathological processes. The aim of             this study was to determine the expression levels of selected miRNA and zinc(II)-related             genes (ZIP-1, BAX, MT2A and MT1A) in the non-tumor PNT1A prostate cell line in             comparison with the prostate cancer cell lines 22Rv1, PC-3 and LNCaP after zinc(II)             treatment. Using bioinformatic approaches we selected miRNAs with putative binding             sites in the 3'UTR regions in Metallothionein 1A and 2A as miRNA 23a, 141, 224,             296-3p, 320, 375 and 376. We observed significantly higher expression of miRNA 23a             in all tumor lines compared to non-tumor PNT1A (13.6-fold in 22Rv1, 7.3-fold in             PC-3, 8.3-fold in LNCaP, p<0.01). We also observed that the 22Rv1 cell line             has significantly higher expression of miRNA 224 in comparison to other cell lines.             In addition, all tumor cell lines expressed significantly higher levels of miRNA 375             in comparison to non-tumor PNT1A (87.1‑fold in 22Rv1, nearly 2,000-fold in PC-3,             56.3‑fold in LNCaP, p<0.01). Nevertheless, miRNA 375 and 23a expression levels             strongly suggest their potential to contribute to the diagnosis of prostate cancer             and miRNA 224 eventually may be suitable for classification of primary tumors.             The expression of miRNA 224 in 22Rv1 cell line was negatively correlated with             increasing zinc(II) concentration only. Our experiments revealed significant negative             correlation of miRNA 376 and MT2A in 22Rv1 and a negative correlation between             miRNA 224 and MT1A in PC-3 cells which may denote possible direct regulation of             MT genes by specific miRNAs in prostate cancer.
Department of Pathological Physiology, Masaryk University, CZ-625 00 Brno, Czech Republic.
Marian
M
Hlavna
Martina
M
Raudenska
Kristyna
K
Hudcova
Jaromir
J
Gumulec
Marketa
M
Sztalmachova
Veronika
V
Tanhäuserova
Petr
P
Babula
Vojtech
V
Adam
Tomas
T
Eckschlager
Rene
R
Kizek
Michal
M
Masarik
D000328:Adult; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D015032:Zinc / Q000378:metabolism*; D019287:Zinc Sulfate / Q000494:pharmacology*
D035683:MicroRNAs; D012333:RNA, Messenger; D019287:Zinc Sulfate; D015032:Zinc