[Actin and mRNA export factors distribution in the nucleus of preovulatory mouse oocytes].
Nuclear actin is a part of the chromatin remodeling complex involved in transcriptional and associated with nascent mRNA, providing them an ability to export from the nucleus. It is currently proved that many factors involved in mRNA editing and mRNA export are localized in IGCs. Using confocal laser scanning microscopy and immunogols labeling technique, we identified actin and A1 hnRNP protein in the nuclei of oocytes from mouse preovulatory follicles. We have demonstrated that nuclear actin is detected in association with the chromatin and IGCs. Components of mRNA export complex including proteins A1 and NXF1/TAP are localized in IGCs together with actin. In accordance with the concept suggesting that IGCs participate in RNA retention in the nucleus we discuss that mRNA transcription-export complex including actin, A1 hnRNP protein and NXF1/TAP, along with mRNA and transcription factors is formed in IGCs.
G N
GN
Pochukalina
V N
VN
Parfenov
D000199:Actins / Q000378:metabolism*; D021581:Active Transport, Cell Nucleus; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D042002:Chromatin Assembly and Disassembly; D005260:Female; D005498:Follicular Phase; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000378:metabolism*; D008074:Lipoproteins / Q000378:metabolism; D051379:Mice; D018613:Microscopy, Confocal; D029543:Nucleocytoplasmic Transport Proteins / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism / Q000648:ultrastructure*; D006080:Ovarian Follicle / Q000378:metabolism / Q000648:ultrastructure; D034443:RNA Transport; D012333:RNA, Messenger / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism
D000199:Actins; D002352:Carrier Proteins; D000076245:Heterogeneous Nuclear Ribonucleoprotein A1; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D008074:Lipoproteins; C569386:NXF1 protein, mouse; D029543:Nucleocytoplasmic Transport Proteins; D012333:RNA, Messenger; D015534:Trans-Activators; C500212:tocopherol-associated protein, mouse