Simple and rapid yeast reporter bioassay for dioxin screening: evaluation of the dioxin-like compounds in industrial and municipal waste incineration plants.
The CROMIS AhR kit, a simple and rapid yeast bioassay kit, was developed and used to detect dioxins and dioxin-like compounds in 20 gas and solid samples collected from refuse incineration plants in Japan. The World Health Organization toxic equivalent (WHO-TEQ) values of the samples were also calculated using high-resolution gas chromatography/high--resolution mass spectrometry. The WHO-TEQ values of the samples varied greatly, ranging from 0.0021-6.3 ng/g to 0.00013-16 ng/m(3)N (normal cubic meter) in the solid and gas samples, respectively. 2,3,4,7,8-Pentachlorodibenzofuran (23478-PeCDF) and 1,2,3,7,8-pentachlorodibenzo-p-dioxin (12378-PeCDD) were the major contributors to the samples' WHO-TEQ values. The yeast in the bioassay responded to these congeners, and the EC50 values of 2,3,7,8-tetrachlorodibenzo-p-dioxin (2378-TeCDD), 12378-PeCDD, and 2,3,4,7,8-PeCDF were 490, 560, and 590 nM, respectively. The incinerator samples were subjected to the bioassay to obtain 2378-TeCDD equivalent values (CROMIS-TEQ values). The CROMIS-TEQ values of the solid and gas samples ranged from 0.0019 to 5.64 ng/g and from 0.14 to 20 ng/m(3)N, respectively. The CROMIS-TEQ and WHO-TEQ values displayed good correlations (r (2) = 0.94 and 0.95 in the solid and gas samples, respectively), except for those of the samples with low dioxin concentrations (below the Japanese emission standards). Therefore, the CROMIS AhR kit is a useful tool for the initial screening of samples containing dioxin-like compounds.
Graduate School of Science and Radiation Research Center, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-Ku, Sakai, Osaka 599-8570, Japan.
Masanobu
M
Kawanishi
Kana
K
Ohnisi
Hidetaka
H
Takigami
Takashi
T
Yagi
D000393:Air Pollutants / Q000032:analysis / Q000378:metabolism*; D001572:Benzofurans / Q000032:analysis / Q000378:metabolism*; D004147:Dioxins / Q000032:analysis / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D017745:Incineration; D007564:Japan; D030781:Organisms, Genetically Modified / Q000378:metabolism; D018336:Receptors, Aryl Hydrocarbon / Q000032:analysis / Q000378:metabolism*; D062611:Solid Waste / Q000032:analysis*; D015003:Yeasts / Q000378:metabolism*
D000393:Air Pollutants; D001572:Benzofurans; D004147:Dioxins; D018336:Receptors, Aryl Hydrocarbon; D062611:Solid Waste