Detection and purification of non-ribosomal peptide synthetase products in Neosartorya fischeri.
Non-ribosomal peptide synthetases (NRPSs) are important enzymes of production machinery for natural products including clinically used antibiotics, antifungal, and anticancer agents. NRPS products are usually further modified by tailoring enzymes, resulting in the formation of diverse structures. We demonstrate here the production and isolation of metabolites produced by two bi-modular NRPSs, i.e., acetylaszonalenin and fumitremorgin-type alkaloids in Neosartorya fischeri NRRL181.
Philipps-Universität Marburg, Marburg, Germany.
Beate
B
Wollinsky
Shu-Ming
SM
Li
D005591:Chemical Fractionation / Q000379:methods*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques; D007211:Indoles / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D055326:Neosartorya / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D010453:Peptide Synthases / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry; D013172:Spores, Fungal / Q000254:growth & development / Q000378:metabolism
D007211:Indoles; D012822:Silicon Dioxide; C010522:tryptoquivaline; D010453:Peptide Synthases; C039286:fumitremorgin B