Modular assembly of protein building blocks to create precisely defined megamolecules.
Enzyme-promoted assembly: The construction of a hetero-bifunctional protein building block, HaloTag-cutinase, that reacts rapidly and selectively with a small-molecule linker is described. The step-wise combination of these building blocks generates a 300 kDa "megamolecule" with precisely defined domain orientation, connectivity, and composition.
Departments of Chemistry and Biomedical Engineering, Howard Hughes Medical Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA.
Justin A
JA
Modica
Stratos
S
Skarpathiotis
Milan
M
Mrksich
D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D015394:Molecular Structure; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D002265:Carboxylic Ester Hydrolases; C019952:cutinase