Efficient non-sterilized fermentation of biomass-derived xylose to lactic acid by a thermotolerant Bacillus coagulans NL01.
Xylose is the major pentose and the second most abundant sugar in lignocellulosic feedstock. Its efficient utilization is regarded as a technical barrier to the commercial production of bulk chemicals from lignocellulosic biomass. This work aimed at evaluating the lactic acid production from the biomass-derived xylose using non-sterilized fermentation by Bacillus coagulans NL01. A maximum lactic acid concentration of about 75 g/L was achieved from xylose of 100 g/L after 72 h batch fermentation. Acetic acid and levulinic acid were identified as important inhibitors in xylose fermentation, which markedly reduced lactic acid productivity at 15 and 1.0 g/L, respectively. But low concentrations of formic acid (<2 g/L) exerted a stimulating effect on the lactic acid production. When prehydrolysate containing total 25.45 g/L monosaccharide was fermented with B. coagulans NL01, the same preference for glucose, xylose, and arabinose was observed and18.2 g/L lactic acid was obtained after 48 h fermentation. These results proved that B. coagulans NL01 was potentially well-suited for producing lactic acid from underutilized xylose-rich prehydrolysates.
College of Chemical Engineering, Nanjing Forestry University, No. 159 Long Pan Road, Nanjing 210037, China. hgouyj@njfu.edu.cn
Jia
J
Ouyang
Cong
C
Cai
Hai
H
Chen
Ting
T
Jiang
Zhaojuan
Z
Zheng
D019342:Acetic Acid / Q000378:metabolism; D000222:Adaptation, Physiological; D001407:Bacillus / Q000378:metabolism* / Q000502:physiology; D018533:Biomass; D005285:Fermentation; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D013696:Temperature; D014994:Xylose / Q000096:biosynthesis*; D003313:Zea mays / Q000737:chemistry
C036909:lignocellulose; D019344:Lactic Acid; C008046:5-hydroxymethylfurfural; D008031:Lignin; D014994:Xylose; D005662:Furaldehyde; D005947:Glucose; D019342:Acetic Acid