Investigation of tumour supressor protein p53 in renal cell carcinoma patients.
BACKGROUND
Investigation of p53 immunoreactivity in formalin-fixed paraffin-embedded tissues of normal renal tissue and renal cell carcinoma with respect to histopathologic subtype and nuclear grade of RCC.


METHODS
42 tissue sections of RCC and 5 samples of normal renal tissue were stained for p53 expression using immunohistochemical assay. The results were analyzed in relation to nuclear grade and histopathologic subtype.


RESULTS
In total, p53 expression was found to be 4 to 5 times higher (30.8%) in other types of RCC than in the clear-cell type of RCC (6.9%). Further, there was no statistically significant difference in p53 overexpression among the histopathologic subtypes (P>0.05, P=0.063). No association was found between the expression of p53 and nuclear grade (P>0.05, P=0.17). Interestingly, our study also showed weak cytoplasmic positivity in renal tubular epithelium.


CONCLUSIONS
Our findings suggest that p53 might play an important role in tumour development or progression and it might be used as a new predictor and therapeutic target for RCC.
Department of Anatomy, P. J. Safarik University, Faculty of Medicine, Kosice, Slovak Republic.
Ingrid
I
Hodorova
Peter
P
Solar
Jozef
J
Mihalik
Janka
J
Vecanova
Marian
M
Adamkov
Silvia
S
Rybarova
D002292:Carcinoma, Renal Cell / Q000737:chemistry* / Q000473:pathology*; D005260:Female; D006801:Humans; D007668:Kidney / Q000737:chemistry; D007680:Kidney Neoplasms / Q000737:chemistry* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D016159:Tumor Suppressor Protein p53