Temperature-induced ultradense PEG polyelectrolyte surface grafting provides effective long-term bioresistance against mammalian cells, serum, and whole blood.
We report a facile method of generating ultradense poly(l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) surface by using high temperature alone, which in turn provides dramatic improvement in resisting nonspecific bioadsorption. X-ray photoelectron spectroscopy (XPS) revealed that the surface graft density increased ~4 times higher on the surface prepared at 80 °C compared to 20 °C. The studies from small-angle X-ray scattering (SAXS) and the effect of varying ionic strength during/post assemblies at 20 and 80 °C indicated that the "cloud point grafting effect" is not the cause for obtaining high density grafting. Stringent long-term bioresistance tests have been conducted and the temperature-induced PLL-g-PEG surfaces have achieved (1) zero mammalian cell adsorption/migration for up to 36 days and (2) extremely close-to-zero protein adsorptions have been observed even after 36 days in 10% serum media and 24 h in whole blood within the ultrasensitive detection limit of time-of-flight secondary ion mass spectrometry (ToF-SIMS).
Interdisciplinary Nanoscience Center (iNANO), Faculty of Science and Technology, School of Dentistry, Aarhus University, Aarhus, Denmark. ryo@inano.au.dk
Ryosuke
R
Ogaki
Ole
O
Zoffmann Andersen
Grethe Vestergaard
GV
Jensen
Kristian
K
Kolind
David Christian Evar
DC
Kraft
Jan Skov
JS
Pedersen
Morten
M
Foss
D000327:Adsorption; D000818:Animals; D001769:Blood; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D009994:Osmolar Concentration; D056951:Photoelectron Spectroscopy; D011092:Polyethylene Glycols / Q000737:chemistry*; D011107:Polylysine / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D013499:Surface Properties; D013696:Temperature; D019737:Transplants
D020099:Coated Materials, Biocompatible; C443714:polylysine-graft-(poly(ethylene glycol)); D011107:Polylysine; D011092:Polyethylene Glycols