Downregulation of L1 perturbs neuronal migration and alters the expression of transcription factors in murine neocortex.
L1 is a cell adhesion molecule associated with a spectrum of human neurological diseases, the most well-known being X-linked hydrocephalus. L1 knockout (L1-KO) mice have revealed a variety of functions of L1 that were crucial in brain development in different brain regions. However; the function of L1 in neuronal migration during cortical histogenesis remains to be clarified. We therefore investigated the corticogenesis of mouse embryos in which L1 molecules were knocked down in selected neurons, by employing in utero electroporation with shRNAs targeting L1 (L1 shRNA). Although more than 50% of the cells transfected with no small hairpin RNA (shRNA; monster green fluorescent protein: MGFP only) vector at embryonic day 13 (E13) reached the cortical plate at E16, significantly fewer (27%) cells transfected with L1 shRNA migrated to the same extent. At E17, 22% of cells transfected with the MGFP-only vector were found in the intermediate zone, and significantly more (34%) cells transfected with L1 shRNA remained in the same zone. Furthermore, the directions of the leading process of neurons transfected with L1 shRNA became more dispersed compared with cells with the MGFP-only vector. In addition, two transcription factors expressed in the neurons, Satb2 and Tbr1, were shown to be reduced or aberrantly expressed in neurons transfected with L1 shRNA. These observations suggest that L1 plays an important role in regulating the locomotion and orientation of migrating neurons and the expression of transcription factors during neocortical development that might partially be responsible for the abnormal tract formation seen in L1-KO mice.
Department of Pathology and Applied Neurobiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto, Japan.
Tomokazu
T
Kishimoto
Kyoko
K
Itoh
Masafumi
M
Umekage
Madoka
M
Tonosaki
Takeshi
T
Yaoi
Kenji
K
Fukui
Vance P
VP
Lemmon
Shinji
S
Fushiki
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D015536:Down-Regulation; D018274:Electroporation; D007150:Immunohistochemistry; D036961:Matrix Attachment Region Binding Proteins / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019579:Neocortex / Q000378:metabolism*; D039842:Neural Cell Adhesion Molecule L1 / Q000235:genetics / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D034741:RNA, Small Interfering; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020825:T-Box Domain Proteins; D014157:Transcription Factors / Q000096:biosynthesis*
D004268:DNA-Binding Proteins; D036961:Matrix Attachment Region Binding Proteins; D039842:Neural Cell Adhesion Molecule L1; D034741:RNA, Small Interfering; C501473:SATB2 protein, mouse; D020825:T-Box Domain Proteins; C492271:Tbr1 protein, mouse; D014157:Transcription Factors