Synchronization using PGF(2α) and estradiol with or without GnRH for timed artificial insemination in dairy cows.
This study examined the use of PGF(2α) and estradiol benzoate (EB) either with or without GnRH to synchronize estrus in dairy cows for timed artificial insemination (TAI) under field conditions. First, Holstein dairy cows with a corpus luteum (CL) received 500 μg cloprostenol and were then randomly allocated to three groups: no further treatment (control, n=236); treatment with 1 mg EB 56 h after cloprostenol (EB group, n=339); or treatment with 1 mg EB 56 h after cloprostenol followed by treatment with 100 μg gonadorelin 24 h later (EB + GnRH group, n=216). All cows received TAI 80 h after the cloprostenol injection. In a second experiment, Holstein dairy cows with a CL received 500 μg cloprostenol and were then randomly allocated to two groups: treatment with 2 mg EB 36 h later (EB group, n=284) or treatment with 2 mg EB 36 h after cloprostenol followed by 100 μg gonadorelin 24 h later (EB + GnRH group, n=229). All cows received TAI 24 h after the EB injection. Logistic analyses revealed that the odds ratio for the probability of pregnancy when 1 mg EB was administered 56 h following cloprostenol was 1.9 and 2.0 times (P<0.001) higher in the EB (39.5%) and EB + GnRH groups (40.7%), respectively, compared with the control group (25.8%). However, pregnancy rates in cows receiving 2 mg EB 24 h following cloprostenol showed no difference compared with cows treated with EB only (32.4%) or with EB + GnRH (35.8%). These results indicate that a synchronization protocol comprising PGF(2α) and EB could be used for TAI in dairy herds under field conditions.
College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, South Korea.
Jae Kwan
JK
Jeong
Hyun Gu
HG
Kang
Tai Young
TY
Hur
Ill Hwa
IH
Kim
D000818:Animals; D002417:Cattle; D003008:Cloprostenol / Q000378:metabolism; D015237:Dinoprost / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D004973:Estrus Synchronization / Q000379:methods*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007315:Insemination, Artificial / Q000379:methods* / Q000662:veterinary; D007774:Lactation; D016017:Odds Ratio; D011247:Pregnancy; D018873:Pregnancy Rate; D011270:Pregnancy, Animal; D012044:Regression Analysis; D013997:Time Factors
D007987:Gonadotropin-Releasing Hormone; D003008:Cloprostenol; D004958:Estradiol; D015237:Dinoprost