Preparation of immunogen-reduced and biocompatible extracellular matrices from porcine liver.
Decellularized biologic matrices are plausible biomedical materials for the bioengineering in liver transplantation. However, one of the concerns for safe medical application is the lack of objective assessment of the immunogen within the materials and the in vivo immune responses to the matrices. The purpose of this study was the production of immunogen-reduced and biocompatible matrices from porcine liver. In the present study, 0.1% SDS solution was effective for removing DNA fragments and sequences encoding possible immunogenic and viral antigens within the matrices. The PCR analysis showed that galactose-α-1,3 galactose β-1,4-N-acetylglucosamine (1,3 gal), swine leukocyte antigen (SLA), and porcine endogenous retrovirus (PERV) were completely removed in the matrices. Collagen and glycosaminoglycans (GAGs) were preserved over 63%-71%, respectively, compared to those of native liver. The implanted decellularized tissues showed minimal host responses and naturally degraded within 10 weeks. In this study, we produced immunogen-reduced and biocompatible extracellular matrices from porcine liver. Although future investigations would be required to determine the mechanism of the host reaction, this study could provide useful information of porcine liver-derived biologic matrices for liver researches.
School of Veterinary Medicine, Stem Cell Institute-KNU, Kangwon National University, Chuncheon, Gangwon 200-701, Republic of Korea. vethouse2000@gmail.com
Kyung-Mee
KM
Park
Sung-Min
SM
Park
Se-Ran
SR
Yang
Seok-Ho
SH
Hong
Heung-Myong
HM
Woo
D000818:Animals; D000941:Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001672:Biocompatible Materials; D002469:Cell Separation; D003094:Collagen / Q000032:analysis; D004247:DNA / Q000032:analysis / Q000235:genetics; D020077:Endogenous Retroviruses / Q000276:immunology / Q000302:isolation & purification; D005109:Extracellular Matrix / Q000276:immunology* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000032:analysis; D006084:Graft Rejection / Q000276:immunology; D048091:Guided Tissue Regeneration / Q000379:methods; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D008099:Liver / Q000166:cytology* / Q000276:immunology*; D016031:Liver Transplantation / Q000276:immunology / Q000379:methods*; D034421:Sus scrofa / Q000276:immunology; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014183:Transplantation, Heterologous / Q000276:immunology / Q000379:methods*
D000941:Antigens; D001672:Biocompatible Materials; D006025:Glycosaminoglycans; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; C438668:swine leukocyte antigen; D003094:Collagen; D004247:DNA