Altered testicular development as a consequence of increase number of sertoli cell in male lambs exposed prenatally to excess testosterone.
The reprograming effects of prenatal testosterone (T) treatment on postnatal reproductive parameters have been studied extensively in females of several species but similar studies in males are limited. We recently found that prenatal T treatment increases Sertoli cell number and reduced spermatogenesis in adult rams. If such disruptions are manifested early in life and involve changes in testicular paracrine environment remain to be explored. This study addresses the impact of prenatal T excess on testicular parameters in infant males, including Sertoli cell number and expression of critical genes [FSH receptor (FSHR), androgen receptor (AR), transforming growth factor beta 1 (TGFB1), 3 (TGFB3), transforming growth factor beta type 1 receptor, (TGFBR1), and anti-Müllerian hormone (AMH)] modulating testicular function. At 4 week of age, male lambs born to dams treated with 30 mg of T propionate twice weekly from day 30 to 90, followed by 40 mg of T propionate from day 90 to 120 of pregnancy (T-males), had a higher number of Sertoli cells/testis (P = 0.035) than control males (C-males) born to dams treated with the vehicle. While no differences were observed in the expression of FSHR and TGFB3, testicular TGFBR1 expression was found to be lower in T-males (P = 0.03) compared to C-males. Expression level of AMH, TGFB1, and AR also tended to be lower in T-males. These findings provide evidence that impact of fetal exposure to T excess is evident early in postnatal life, mainly characterized by an increase in Sertoli cell number. This could explain the testicular dysfunction observed in adult rams.
Laboratory of Animal Physiology and Endocrinology, Faculty of Veterinary Sciences, University of Concepción, Av. Vicente Mendez 595, Chillán, Chile. pedro.rojas@udec.cl
Pedro P
PP
Rojas-García
Mónica P
MP
Recabarren
Teresa
T
Sir-Petermann
Rodolfo
R
Rey
Sergio
S
Palma
Albert
A
Carrasco
Carlos C
CC
Perez-Marin
Vasantha
V
Padmanabhan
Sergio E
SE
Recabarren
D000818:Animals; D002452:Cell Count; D005260:Female; D047109:Fetal Development / Q000187:drug effects; D005640:Follicle Stimulating Hormone / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012756:Sheep; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development; D013739:Testosterone / Q000097:blood / Q000494:pharmacology*; D053782:Transforming Growth Factor beta3 / Q000235:genetics / Q000378:metabolism
D011944:Receptors, Androgen; D011962:Receptors, FSH; D053782:Transforming Growth Factor beta3; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone