High-throughput quantitative imaging of cell spreading dynamics by multi-step microscopy projection photolithography based on a cell-friendly photoresist.
A new method for the high-throughput study of cell spreading dynamics is devised by multi-step microscopy projection photolithography based on a cell-friendly photoresist. By releasing a large number of rounded cells in single cell arrays and monitoring their spreading dynamics by interference reflection microscopy, a large number of cell spreading data can be acquired by a single experiment.
Department of Mechanical Engineering, POSTECH, San31, Hyoja-dong, Nam-Gu, Pohang, 790-784, Gyeongbuk, Korea. muric@postech.ac.kr
Jong-Cheol
JC
Choi
Junsang
J
Doh
D048430:Cell Shape / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D046888:Tissue Array Analysis / Q000379:methods*