Antibacterial efficacy of conventional and single-use Ni-Ti endodontic instruments: an in vitro microbiological evaluation.
OBJECTIVE
The purpose of this in vitro study was to investigate the capability of different Ni-Ti instruments in disinfecting root canals previously infected with E. faecalis.


METHODS
60 single-rooted, freshly extracted teeth were selected, sterilized and inoculated with cells of E. faecalis strain. All root canals were shaped with different Ni-Ti instruments (MTwo, Revo-S, Reciproc, and OneShape) under irrigation with NaOCl and 17% EDTA solutions by the same trained operator. The number of Colony Forming Units (CFU)/ml of E. faecalis was evaluated for each group. Numeric data were submitted to statistical analysis.


RESULTS
MTwo, REVO-S, Reciproc and OneShape reported significantly lower CFU/ml values and showed no significant differences among them (p>0.05). All groups showed significantly higher CFU/ml values than not treated samples (negative control).


CONCLUSIONS
All the Ni-Ti instrumentation efficiently reduced E. faecalis counts and no significant differences were found among them. Single-use files can offer antibacterial results comparable with traditional instruments.
Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, School of Dentistry, University of Pavia, Pavia, Italy.
Alberto
A
Dagna
Carla Renata
CR
Arciola
Livia
L
Visai
Laura
L
Selan
Marco
M
Colombo
Stefano
S
Bianchi
Claudio
C
Poggio
D000890:Anti-Infective Agents / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D003762:Dental Instruments; D003786:Dental Pulp Cavity / Q000382:microbiology*; D004209:Disposable Equipment; D004492:Edetic Acid / Q000494:pharmacology; D004708:Endodontics / Q000295:instrumentation*; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D004867:Equipment Design; D017743:Equipment Reuse; D009532:Nickel; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000295:instrumentation*; D012973:Sodium Hypochlorite / Q000494:pharmacology; D014025:Titanium; D014081:Tooth Extraction
D000890:Anti-Infective Agents; D002614:Chelating Agents; D012388:Root Canal Irrigants; C040654:titanium nickelide; D009532:Nickel; D004492:Edetic Acid; D014025:Titanium; D012973:Sodium Hypochlorite