The thyroid hormone receptor alpha locus and white matter lesions: a role for the clock gene REV-ERBα.
BACKGROUND
Thyroid disorders are associated with an increased risk of cognitive impairment and Alzheimer's disease. Both small vessel disease and neurodegeneration have a role in the pathogenesis of cognitive impairment and Alzheimer's disease. Thyroid hormone receptor alpha (TRα) is the predominant TR in brain. The circadian clock gene REV-ERBα overlaps with the TRα gene and interferes with TRα expression. Limited data are available on the role of the TRα/REV-ERBα locus in small vessel disease and neurodegeneration. We therefore studied genetic variation in the TRα/REV-ERBα locus in relation to brain imaging data, as early markers for small vessel disease and neurodegeneration.


METHODS
Fifteen polymorphisms, covering the TRα/REV-ERBα locus, were studied in relation to white matter lesion (WML), total brain, and hippocampal volumes in the Rotterdam Study I (RS-I, n=454). Associations that remained significant after multiple testing correction were subsequently studied in an independent population for replication (RS-II, n=607).


RESULTS
No associations with total brain or hippocampal volumes were detected. A haplotype block in REV-ERBα was associated with WML volumes in RS-I. Absence of this haplotype was associated with larger WML volumes in women (0.38%±0.18% [β±SE], p=0.007), but not in men (0.04%±0.11%, p=0.24), which was replicated in RS-II (women: 0.15%±0.05%, p=0.04; men: 0.05%±0.07%, p=0.80). Meta-analysis of the two populations showed that women lacking this haplotype have a 1.9 times larger WML volume (p=0.001).


CONCLUSIONS
Our results suggest a role for REV-ERBα in the pathogenesis of WMLs.
Department of Internal Medicine, Erasmus Medical Center, Rotterdam, The Netherlands.
Marco
M
Medici
M Arfan
MA
Ikram
Fedde
F
van der Lijn
Tom
T
den Heijer
Meike W
MW
Vernooij
Albert
A
Hofman
Wiro J
WJ
Niessen
Theo J
TJ
Visser
Monique M B
MM
Breteler
Robin P
RP
Peeters
D001921:Brain / Q000033:anatomy & histology / Q000473:pathology; D005260:Female; D006239:Haplotypes; D006801:Humans; D056784:Leukoencephalopathies / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1 / Q000235:genetics* / Q000502:physiology; D011110:Polymorphism, Genetic; D037021:Thyroid Hormone Receptors alpha / Q000235:genetics
D056953:Nuclear Receptor Subfamily 1, Group D, Member 1; D037021:Thyroid Hormone Receptors alpha