Sunitinib-associated pseudothrombocytopenia induced by IgM antibody.
Thrombocytopenia is a well-documented adverse reaction of sunitinib. Thrombocytopenia was observed in a patient with metastatic renal clear-cell carcinoma undergoing sunitinib treatment. Platelet count in an ethylenediaminetetraacetic acid (EDTA) sample was 19 × 10(9)/l. To exclude pseudothrombocytopenia (PTCP), a platelet count in citrate-anticoagulated blood was performed, showing a platelet count of 6 × 10(9)/l. Due to the apparent thrombocytopenia, the patient received platelet concentrates. Subsequent analyses revealed PTCP whereby platelet clumping was most abundant in citrate - followed by EDTA- and heparin-anticoagulated blood samples. This effect was partially reversed after placing blood samples at 37°C. The IgM antiplatelet autoantibodies responsible for in vitro agglutination are temperature and multianticoagulant dependent and did not react to amikacin pre-supplementation. Remarkably, the antibody revealed specificity to platelet antigens other than GPIIb/IIIa, GPIb/IX, GPIa/IIa, GPIV, and GPV. After 16 days of discontinuing sunitinib, no PTCP and no platelet reactive antibodies could be detected. We report a case of PTCP with clear time-relation with sunitinib, strongly suggesting the mechanism to be sunitinib dependent. Since this finding has not been described before, non-recognition of PTCP during sunitinib treatment might lead to dose reduction or unwarranted therapy.
Department of Clinical Chemistry and Hematology, Sint Franciscus Gasthuis , Rotterdam , The Netherlands.
Arjan
A
Albersen
Leendert
L
Porcelijn
Joyce
J
Schilders
Hanneke
H
Zuetenhorst
Tjin
T
Njo
Paul
P
Hamberg
D006801:Humans; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology*; D007211:Indoles / Q000008:administration & dosage / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation; D011758:Pyrroles / Q000008:administration & dosage / Q000009:adverse effects*; D000077210:Sunitinib; D013921:Thrombocytopenia / Q000097:blood / Q000139:chemically induced* / Q000276:immunology*
D007075:Immunoglobulin M; D007211:Indoles; D011758:Pyrroles; D000077210:Sunitinib