Biodegradable photo-crosslinked alginate nanofibre scaffolds with tuneable physical properties, cell adhesivity and growth factor release.
Nanofibrous scaffolds are of interest in tissue engineering due to their high surface area to volume ratio, interconnected pores, and architectural similarity to the native extracellular matrix. Our laboratory recently developed a biodegradable, photo-crosslinkable alginate biopolymer. Here, we show the capacity of the material to be electrospun into a nanofibrous matrix, and the ability to enhance cell adhesion and proliferation on these matrices by covalent modification with cell adhesion peptides. Additionally, the potential of covalently incorporating heparin into the hydrogels during the photopolymerisation process to sustain the release of a heparin binding growth factor via affinity interactions was demonstrated. Electrospun photo-crosslinkable alginate nanofibrous scaffolds endowed with cell adhesion ligands and controlled delivery of growth factors may allow for improved regulation of cell behaviour for regenerative medicine.
Department of Biomedical Engineering and Orthopaedic Surgery, Case Western Reserve University, Cleveland, OH 44106, USA.
Sung In
SI
Jeong
Oju
O
Jeon
Melissa D
MD
Krebs
Michael C
MC
Hill
Eben
E
Alsberg
D020341:Absorbable Implants; D000464:Alginates / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000737:chemistry; D005347:Fibroblasts / Q000502:physiology; D020723:Glucuronic Acid / Q000737:chemistry; D006493:Heparin / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D058105:Polymerization; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014466:Ultraviolet Rays
D000464:Alginates; D006603:Hexuronic Acids; D016222:Fibroblast Growth Factor 2; D020723:Glucuronic Acid; D006493:Heparin