Treatment of agro-industrial wastewater using microalgae-bacteria consortium combined with anaerobic digestion of the produced biomass.
Two combined processes were studied in order to produce second generation biofuels: microalgae biomass production and its further use to produce biogas. Two 5 L photobioreactors for treating wastewater from a potato processing industry (from now on RPP) and from a treated liquid fraction of pig manure (from now on RTE) were inoculated with Chlorella sorokiniana and aerobic bacteria at 24±2.7 °C and 6000 lux for 12 h per day of light supply. The maximum biomass growth was obtained for RTE wastewater, with 26.30 mg dry weight L(-1) d(-1). Regarding macromolecular composition of collected biomass, lipid concentration reached 30.20% in RPP and 4.30% in RTE. Anaerobic digestion results showed that methane yield was highly influenced by substrate/inoculum ratio and by lipids concentration of the biomass, with a maximum methane yield of 518 mL CH4 g COD(-1)added using biomass with a lipid content of 30% and a substrate/inoculum ratio of 0.5.
Agricultural Technological Institute of Castilla y León, Ctra. Burgos, km. 119, 47071 Valladolid, Spain. hergonda@itacyl.es
D
D
Hernández
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B
Riaño
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M
Coca
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MC
García-González
D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D058440:Denitrification; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000032:analysis*; D008697:Methane / Q000378:metabolism; D058086:Microalgae / Q000378:metabolism*; D058465:Nitrification; D010100:Oxygen / Q000032:analysis; D010758:Phosphorus / Q000302:isolation & purification; D058433:Photobioreactors / Q000382:microbiology; D000644:Quaternary Ammonium Compounds / Q000302:isolation & purification; D013552:Swine; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
D007220:Industrial Waste; D008697:Methane; D010100:Oxygen; D010758:Phosphorus; D000644:Quaternary Ammonium Compounds; D062065:Wastewater