Increased sensitivity to chloramphenicol by inactivation of manB in Streptomyces coelicolor.
Phosphomannomutase (ManB) is involved in the biosynthesis of GDP-mannose, which is vital for numerous processes such as synthesis of carbohydrates, production of alginates and ascorbic acid, and post-translational modification of proteins. Here, we discovered that a deletion mutant of manB (BG101) in Streptomyces coelicolor (S. coelicolor) showed higher sensitivity to bacteriostatic chloramphenicol (CM) than the wild-type strain (M145), along with decreased production of CM metabolites. Deletion of manB also decreased the mRNA expression level of drug efflux pumps (i.e., cmlR1 and cmlR2) in S. coelicolor, resulting in increased sensitivity to CM. This is the first report on changes in antibiotic sensitivity to CM by deletion of one glycolysis-related enzyme in S. coelicolor, and the results suggest different approaches for studying the antibiotic-resistant mechanism and its regulation.
Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Korea.
Thangamani
T
Rajesh
Eunjung
E
Song
Bo-Rahm
BR
Lee
Sung-Hee
SH
Park
Jong-Min
JM
Jeon
Eunjung
E
Kim
Changmin
C
Sung
Jae-Hun
JH
Lee
Dongwon
D
Yoo
Hyung-Yeon
HY
Park
Yun-Gon
YG
Kim
Byung-Gee
BG
Kim
Yung-Hun
YH
Yang
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008359:Mannose-6-Phosphate Isomerase / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests / Q000379:methods; D050296:Microbial Viability; D009097:Multienzyme Complexes / Q000235:genetics / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D010733:Phosphoglucomutase / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D017875:Phosphotransferases (Phosphomutases) / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D002701:Chloramphenicol; C093300:ManB protein, bacteria; D009713:Nucleotidyltransferases; D008359:Mannose-6-Phosphate Isomerase; D017875:Phosphotransferases (Phosphomutases); D010733:Phosphoglucomutase; C052799:phosphomannomutase