New immunoenzymatic strategy for rapid and selective growth of Salmonella.
We demonstrated that an exogenous conjugate consisting of a β-lactamase and a monoclonal antibody against a surface antigen of Salmonella is capable of conferring selective penicillin resistance on bacteria. This immunoenzymatic approach allows the rapid growth and detection of specific bacteria and may find other applications when temporary and nongenetic antibiotic resistance is required.
Biochimie et Génétique Moléculaire Bactérienne, Institut des Sciences de la Vie, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.
Isabelle
I
Ponsard
Patrice
P
Soumillion
D000907:Antibodies, Bacterial / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001431:Bacteriological Techniques / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D010403:Penicillin Resistance; D010406:Penicillins / Q000494:pharmacology*; D012475:Salmonella / Q000302:isolation & purification*; D013194:Staining and Labeling / Q000379:methods*; D001618:beta-Lactamases / Q000378:metabolism*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D010406:Penicillins; D001618:beta-Lactamases