Bioluminescent enzyme immunoassay for the detection of norovirus capsid antigen.
An ultrasensitive and fully automated bioluminescent enzyme immunoassay (BLEIA) was developed for the detection of norovirus (NV) capsid antigen. In the evaluation tests with recombinant virus-like particles, the BLEIA demonstrated broad reactivity against several NV genotypes (genotypes 1, 3, 4, 7, 8, and 12 in genogroup I [GI] and genotypes 1, 2, 3, 4, 5, 6, 12, and 13 in GII), a wide dose-response range from 0.25 pg/ml to 10,000 pg/ml, and good reproducibility with low coefficients of variation (CVs) (within-run CVs of <2.8%, between-day CVs of <3.7%). In the evaluation tests with NV-positive fecal samples, a good correlation (y = 0.66x - 3.21, r = 0.84) between the BLEIA and real-time quantitative reverse transcription-PCR was obtained. Furthermore, in the dilution test with NV specimens, the analytical sensitivity of NV was estimated to be 10(5) to 10(6) copies/g of fecal sample, indicating that the analytical sensitivity of the BLEIA is comparable to that of commercially available molecular methods. All assay steps are fully automated, the turnaround time is 46 min, and the throughput of the assay is 120 tests/h. These results indicate that the BLEIA is potentially useful for the rapid diagnosis of NV in epidemic and sporadic gastroenteritis.
Biochemical Research Laboratory I, Eiken Chemical Co., Ltd., Nogi-Machi, Shimotsuga-Gun, Tochigi Prefecture, Japan. Nozomi_Sakamaki@eiken.co.jp
Nozomi
N
Sakamaki
Yoshiyuki
Y
Ohiro
Mitsuki
M
Ito
Mitsuru
M
Makinodan
Tsubasa
T
Ohta
Wataru
W
Suzuki
Susumu
S
Takayasu
Harufumi
H
Tsuge
D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D057205:Automation, Laboratory / Q000379:methods; D017250:Caliciviridae Infections / Q000175:diagnosis* / Q000821:virology; D036022:Capsid Proteins / Q000032:analysis* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007223:Infant; D007231:Infant, Newborn; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D029322:Norovirus / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods
D000956:Antigens, Viral; D036022:Capsid Proteins