[Potential endocrine disrupting effects of bifenthrin in rats].
OBJECTIVE
To study the potential endocrine disrupting effects of bifenthrin (BIF) by using uterotrophic assay and Hershberger assay.


METHODS
In uterotrophic assay, 60 female SD rats were randomly divided into 6 groups, 10 rats per group. Rats in bifenthrin-treated groups were given different doses of bifenthrin (1.47, 4.41 and 13.23 mg/kg BW by gavage for 3 consecutive days). Rats in negative control groups were given corn oil by gavage. Rats in ethinyl estradiol (EE) oral positive control groups were given EE 1.0 microg/kg BW by gavage. Rats in EE injected positive groups were given 0.6 microg/kg BW EE by subcutaneously injection while given corn oil by gavage. At necropsy, the wet and blotted uteri were weighed. The relative uteri weights were calculated, and the histology of uteri was observed. In Hershberger assay, 60 castrated male SD rats were randomly divided into 6 groups, with 10 rats in each group. Rats in BIF-treated groups were given different doses of BIF (1.4, 4.2 and 12.6 mg/kg BW) by gavage. Flutamide (3.0 mg/kg BW) were given to animals in the positive control group by gavage. Rats in the negative control group and testosterone propionate group were given corn oil by gavage for 10 consecutive days. Rats in all groups except the negative control group were also treated with testosterone propionate (TP, 0.2 mg/kg BW) by subcutaneous injection. At necropsy, ventral prostate (VP), seminal vesicle plus fluids and coagulating glands (SVCG), levator ani-bulbocavernosus muscle (LABC), paired Cowper's glands (COW) and the glands penis (GP), liver, kidneys, adrenals were weighed. Serum triiodothyronine (T3) and thyroxine (T4) were determined.


RESULTS
In uterotrophic assay, compared with the negative control group, the mean relative wet weight and relative blotted weight of uterine were increased significantly in the female rats given by BIF at 13.23 mg/kg BW for 3 days (P < 0.05). BIF resulted in a significant increase of epithelial cell heights of uteri at 4.41 and 13.23 mg/kg BW, as compared with control group. In Hershberger assay, compared with the TP group, mean absolute weight and relative weight of VP and LABC in BIF high dose group were significant decreased. Moreover, total T3 and T4 level were decreased also (P < 0.05).


CONCLUSIONS
Uterotrophic assay and Hershberger assay used in this study showed that BIF may have endocrine disrupting properties.
National Institute for Nutrition and Food Safety, China CDC, Beijing 100021, China. tanyanjun716@163.com
Yanjun
Y
Tan
Hengjuan
H
Wang
Yan
Y
Song
Hui
H
Yang
Xudong
X
Jia
Ning
N
Li
D000311:Adrenal Glands; D000726:Androgen Antagonists; D000818:Animals; D001835:Body Weight; D052244:Endocrine Disruptors / Q000633:toxicity*; D005260:Female; D006801:Humans; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects*; D008297:Male; D009929:Organ Size; D011722:Pyrethrins / Q000633:toxicity*; D051381:Rats; D043343:Testosterone Propionate; D013974:Thyroxine; D014284:Triiodothyronine; D014599:Uterus / Q000033:anatomy & histology / Q000187:drug effects*
D000726:Androgen Antagonists; D052244:Endocrine Disruptors; D011722:Pyrethrins; D014284:Triiodothyronine; C099952:bifenthrin; D013974:Thyroxine; D043343:Testosterone Propionate