RNA polymerase III repression by the retinoblastoma tumor suppressor protein.
The retinoblastoma (RB) tumor suppressor protein regulates multiple pathways that influence cell growth, and as a key regulatory node, its function is inactivated in most cancer cells. In addition to its canonical roles in cell cycle control, RB functions as a global repressor of RNA polymerase (Pol) III transcription. Indeed, Pol III transcripts accumulate in cancer cells and their heightened levels are implicated in accelerated growth associated with RB dysfunction. Herein we review the mechanisms of RB repression for the different types of Pol III genes. For type 1 and type 2 genes, RB represses transcription through direct contacts with the core transcription machinery, notably Brf1-TFIIIB, and inhibits preinitiation complex formation and Pol III recruitment. A contrasting model for type 3 gene repression indicates that RB regulation involves stable and simultaneous promoter association by RB, the general transcription machinery including SNAPc, and Pol III, suggesting that RB may impede Pol III promoter escape or elongation. Interestingly, analysis of published genomic association data for RB and Pol III revealed added regulatory complexity for Pol III genes both during active growth and during arrested growth associated with quiescence and senescence. This article is part of a Special Issue entitled: Transcription by Odd Pols.
Department of Biochemistry & Molecular Biology, Michigan State University, 603 Wilson Road, East Lansing, MI 48824, USA.
Alison
A
Gjidoda
R William
RW
Henry
D000818:Animals; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012320:RNA Polymerase III / Q000235:genetics / Q000378:metabolism*; D019572:Retinal Neoplasms / Q000378:metabolism; D012175:Retinoblastoma / Q000378:metabolism; D016160:Retinoblastoma Protein / Q000378:metabolism*; D014158:Transcription, Genetic
D016160:Retinoblastoma Protein; D012320:RNA Polymerase III