A novel fibrous scaffold composed of electrospun porous poly (epsilon-caprolactone) fibers for bone tissue engineering.
In this study, porous poly(ε-caprolactone) (PCL) fiber-based fibrous scaffolds are created using a suitable ratio of dimethyl chloride and acetone by using electrospinning. With the porous structure, it induced CaP particles to easily coat on the fibers after immersion in simulated body fluid solution. The morphology of the electrospun membranes was observed using scanning electron microscopic observation. The results showed that the CaP coated successfully, as examined by scanning electron microscopy, energy-dispersive spectroscopy, X-ray diffraction, transmission electron microscopic techniques and mass comparative analysis. The wettability of the coated fibrous scaffolds was tested using contact angle analysis. The in vitro cellular proliferation and cell interaction with fibrous scaffolds were investigated. In addition, the in vivo bone formation capacity of fibrous scaffolds including the non-porous (PCL-DCM), porous (PCL-DCM/Ace) and CaP coating on PCL/DCM-Ace for 2, 4, 8 and 12 h immersed in SBF solution were also investigated. By measuring the in vitro results, we verified that porous PCL fiber-based fibrous scaffold after 12 h of immersion in simulated body fluid (PCL-DCM/Ace-12) was excellent for cell interaction, growth and proliferation. The in vivo analyses showed that the PCL-DCM/Ace-12 fibrous scaffold enabled greater acceleration of bone formation than PCL/DCM and PCL/DCM-Ace fibrous scaffolds.
1Department of Biomedical Engineering and Materials, College of Medicine, Soonchunhyang University, Cheonan, Chungnam, Republic of Korea.
Thi-Hiep
TH
Nguyen
Trinh Quang
TQ
Bao
Ihho
I
Park
Byong-Taek
BT
Lee
D001846:Bone Development; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D011091:Polyesters / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
D011091:Polyesters; C016240:polycaprolactone