Comprehension of viscous morphology--evaluation of fractal and conventional parameters for rheological characterization of Aspergillus niger culture broth.
The filamentous fungus Aspergillus niger is a widely used host in industrial processes from food, chemical to pharmaceutical industry. The most prominent feature of this filamentous microorganism in submerged cultivation is its complex morphology which comprises dense spherical pellets as well as viscous elongated filaments. Depending on culture conditions, the exhibited morphology has tremendous effect on the overall process, making a precise understanding of fungal growth and morphology indispensable. Morphology, however, is only industrially relevant as long as it can be linked to important cultivation characteristics of filamentous microorganisms such as culture broth flow behavior. In the present study, different conventional and fractal morphological parameters gained from automatic image analysis were tested for their eligibility to predict culture broth rheology from morphologic appearance. The introduced biomass independent rheological parameters K(BDW) and n(BDW) obtained by power law relationship were successfully estimated from morphology related fractal and conventional parameters. For improved characterization of morphologic appearance of filamentous fungi newly introduced fractal quotient and lacunarity were compared to conventional particle shape parameters in form of the earlier established Morphology number (MN).
Institute of Biochemical Engineering, Technische Universität Braunschweig, Gaußstraße 17, 38106 Braunschweig, Germany.
Thomas
T
Wucherpfennig
Antonia
A
Lakowitz
Rainer
R
Krull
D001234:Aspergillus niger / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D017709:Fractals; D007091:Image Processing, Computer-Assisted; D008954:Models, Biological; D025282:Mycelium; D012212:Rheology / Q000379:methods*; D014783:Viscosity
D003470:Culture Media