Effects of depleted uranium on oxidative stress, detoxification, and defence parameters of zebrafish Danio rerio.
In this study, we investigated the effects of depleted uranium (DU), the by-product of nuclear enrichment of uranium, on several parameters related to oxidative stress, detoxification, and the defence system in the zebrafish Danio rerio. Several parameters were recorded: phenoloxidase-like (PO) activity, reactive oxygen species (ROS) production, and 7-ethoxyresrufin-O-deethylase (EROD) activity. Experiments were performed on adult and larvae D. rerio. Adult fish were exposed for 28 days at 20 μg U/L followed by a 27-day depuration period. Eggs of D. rerio were exposed for 4 days at 0, 20, 100, 250, 500, and 1,000 μg U/L. Results showed that DU increased ROS production both in adult and in larvae even at the low concentrations tested and even during the depuration period for adult D. rerio. DU also modified PO-like activity, both in the D. rerio adult and larvae experiments, but in a more transient manner. EROD activity was not modified by DU, but sex effects were shown. Results are discussed by way of comparison with other known effects of uranium in fish. Overall, these results show that the mechanisms of action of DU in fish tend to be similar to the ones existing for mammals. These results encourage the development and use of innate immune biomarkers to understand the effects of uranium and, more generally, radionuclides on the fish immune system.
Institut de Radioprotection et Sûreté Nucléaire, PRP-ENV/SERIS/LECO, Laboratoire d'Ecotoxicologie des Radionucléides, 13115, St-Paul-lez-Durance Cedex, France. beatrice.gagnaire@irsn.fr
Beatrice
B
Gagnaire
Isabelle
I
Cavalie
Virginie
V
Camilleri
Christelle
C
Adam-Guillermin
D000818:Animals; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D005260:Female; D008658:Inactivation, Metabolic; D008297:Male; D014442:Monophenol Monooxygenase / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D014501:Uranium / Q000633:toxicity*; D014875:Water Pollutants, Radioactive / Q000633:toxicity*; D015027:Zebrafish / Q000502:physiology
D014875:Water Pollutants, Radioactive; D014501:Uranium; D019363:Cytochrome P-450 CYP1A1; D014442:Monophenol Monooxygenase