Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV).
A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome.
Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Camino 60 cuadras Km 5,5, X5020ICA, Córdoba, Argentina. nicobejerman@gmail.com
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N
Bejerman
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Giolitti
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de Breuil
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Lenardon
D015153:Blotting, Western; D003001:Cloning, Molecular; D016679:Genome, Viral; D006368:Helianthus / Q000821:virology; D008854:Microscopy, Electron; D010935:Plant Diseases / Q000821:virology; D017800:Potyvirus / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D012367:RNA, Viral / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D036226:Seedlings / Q000821:virology; D014026:Nicotiana / Q000821:virology
D012367:RNA, Viral