In vitro biological control of infective larvae of Ancylostoma ceylanicum.
The aim of this study was to evaluate the predatory activity of the fungus Duddingtonia flagrans (AC001) on infective larvae of Ancylostoma ceylanicum after gastrointestinal transit in hamsters. Twenty animals were used in the experiment, divided into two groups: a treated group (10 animals) and a control group (10 animals). In the group treated with D. flagrans, each animal received mycelium from the AC001 isolate, at an oral dose of 5 mg/25 g of live weight. To evaluate the predatory activity of the fungus, fecal samples were collected from the animals in both groups, at the times of 6, 8, 12, 24 and 36 hours after the treatment. Then, subsamples of 2 g of feces were placed in Petri dishes containing 2% water-agar (2% WA) culture medium and 1000 L3 of A. ceylanicum. Over the study period, the following percentage reductions were observed: 43.2% (6 hours), 30.8% (8 hours), 25.8% (12 hours), 30% (24 hours) and 11% (36 hours). The fungus D. flagrans presented predatory activity on the L3 of A. ceylanicum, after passing through the hamsters' gastrointestinal tract. It was therefore concluded that the fungus D. flagrans may be an alternative for biological control of the L3 of A. ceylanicum.
Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brasil. fernandauss@hotmail.com
Fernanda Mara
FM
Fernandes
Jackson Victor
JV
Araújo
Fabio Ribeiro
FR
Braga
Pedro Henrique
PH
Gazzinelli-Guimarães
Juliana Milani
JM
Araujo
Sebastião Rodrigo
SR
Ferreira
Rogério Oliva
RO
Carvalho
Ingrid Ney Kramer
IN
de Mello
Ricardo Toshio
RT
Fujiwara
D000722:Ancylostoma; D000818:Animals; D061046:Biological Control Agents; D006224:Cricetinae; D059586:Duddingtonia; D007814:Larva
D061046:Biological Control Agents