Endocrine disruptive actions of inhaled benzo(a)pyrene on ovarian function and fetal survival in fisher F-344 adult rats.
This study evaluated the effect of inhaled BaP on female reproductive function. Rats were exposed to 50, or 75 or 100 μg BaP/m(3), 4 h a day for 14 days via inhalation. Plasma E(2), P(4), LH and FSH concentrations were determined. Ovarian BaP metabolism and aryl hydrocarbon hydrolase (AHH) activity at proestrus were determined and fertility evaluations were conducted. Ovulation rate and number of pups/litter were reduced in rats exposed to 100 μg BaP/m(3) compared with other treatment and control groups. Plasma concentrations of E(2), and LH were significantly reduced at proestrus in BaP-exposed versus those of controls whereas those of P(4) were significantly reduced at diestrus I. The activity of AHH in ovarian and liver tissues and concentrations of BaP 7,8-diol and BaP 3,6-dione metabolites increased in an exposure concentration-dependent manner. These data suggest that exposure of rats to BaP prior to mating contributes to reduced ovarian function and fetal survival.
Department of Physiology, Meharry Medical College, Nashville, TN 37208, United States. aarchibong@mmc.edu
Anthony E
AE
Archibong
Aramandla
A
Ramesh
Frank
F
Inyang
Mohammad S
MS
Niaz
Darryl B
DB
Hood
Prapaporn
P
Kopsombut
D000280:Administration, Inhalation; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D052244:Endocrine Disruptors / Q000633:toxicity*; D004958:Estradiol; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D005298:Fertility / Q000187:drug effects; D005333:Fetus / Q000187:drug effects*; D005640:Follicle Stimulating Hormone / Q000097:blood; D008098:Litter Size / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D010053:Ovary / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D010060:Ovulation / Q000187:drug effects; D011374:Progesterone / Q000097:blood; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D052244:Endocrine Disruptors; D001564:Benzo(a)pyrene; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D001189:Aryl Hydrocarbon Hydroxylases