Effect of Sevoflurane postconditioning on gene expression in brain tissue of the middle cerebral artery occlusion rat model.
Ischemic postconditioning has been described in both heart and brain. The first aim of this study was to evaluate the effects of Sevoflurane postconditioning (SP) on brain biochemical parameters, Bcl-2, Bax, c-Fos and Caspase-3 protein levels and Bcl-2, Bax, TNF-α and Caspase-3 mRNA expression in the middle cerebral artery occlusion model. Results showed that SP markedly decreased cerebral oxidative injury and improved immunity activity. In addition, SP significantly enhanced cerebral Bcl-2, c-Fos and decreased Bax, Caspase-3 proteins positive expression. Reverse transcription polymerase chain reaction analysis showed that SP markedly enhanced Bcl-2, and decreased Bax, TNF-α and Caspase-3 mRNA expression. Our results confirm that SP can play the protective action against cerebral ischemia reperfusion-induced brain injury by regulating cerebral antioxidant enzymes activities, Bcl-2, Bax, c-Fos and Caspase-3 protein positive expression levels and Bcl-2, Bax, TNF-α and Caspase-3 mRNA expression.
Department of Anesthesiology, Tianjin Huanhu Hospital, Tianjin, 300060, China.
Hai-Gen
HG
Liu
Zhen
Z
Hua
Yan
Y
Zhang
Ya-Xin
YX
Wang
Chun
C
Meng
Yu
Y
Liang
Shou-Yuan
SY
Tian
Yi-Ping
YP
Ma
Liang
L
Wang
Wen-Sheng
WS
Wang
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D002545:Brain Ischemia / Q000235:genetics / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects*; D020244:Infarction, Middle Cerebral Artery / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D053583:Interleukin-1beta / Q000378:metabolism; D057775:Ischemic Postconditioning; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008738:Methyl Ethers / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000077149:Sevoflurane; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
D053583:Interleukin-1beta; D008738:Methyl Ethers; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D051028:bcl-2-Associated X Protein; D009569:Nitric Oxide; D000077149:Sevoflurane; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D053148:Caspase 3