T-cell receptor repertoires of tumor-infiltrating lymphocytes after hyperthermia using functionalized magnetite nanoparticles.
OBJECTIVE
Accumulating evidence has indicated that hyperthermia using magnetite nanoparticles induces antitumor immunity. This study investigated the diversity of T-cell receptors (TCRs) in tumor-infiltrating lymphocytes after hyperthermia using magnetite nanoparticles.


METHODS
Functionalized magnetite nanoparticles, N-propionyl-4-S-cysteaminylphenol (NPrCAP)/magnetite, were synthesized by conjugating the melanogenesis substrate NPrCAP with magnetite nanoparticles. NPrCAP/magnetite nanoparticles were injected into B16 melanomas in C57BL/6 mice, which were subjected to an alternating magnetic field for hyperthermia treatment.


RESULTS
Enlargement of the tumor-draining lymph nodes was observed after hyperthermia. The TCR repertoire was restricted in tumor-infiltrating lymphocytes, and expansion of Vβ11(+) T cells was preferentially found. DNA sequences of the third complementaritydetermining regions revealed the presence of clonally expanded T cells.


CONCLUSIONS
These results indicate that the T-cell response in B16 melanomas after hyperthermia is dominated by T cells directed toward a limited number of epitopes and that epitope-specific T cells frequently use a restricted TCR repertoire.
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. akira@chem-eng.kyushu-u.ac.jp
Akira
A
Ito
Masaki
M
Yamaguchi
Noriaki
N
Okamoto
Yuji
Y
Sanematsu
Yoshinori
Y
Kawabe
Kazumasa
K
Wakamatsu
Shosuke
S
Ito
Hiroyuki
H
Honda
Takeshi
T
Kobayashi
Eiichi
E
Nakayama
Yasuaki
Y
Tamura
Masae
M
Okura
Toshiharu
T
Yamashita
Kowichi
K
Jimbow
Masamichi
M
Kamihira
D000818:Animals; D005260:Female; D006979:Hyperthermia, Induced / Q000379:methods*; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000473:pathology; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D008546:Melanoma, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
D058185:Magnetite Nanoparticles; D011948:Receptors, Antigen, T-Cell