Saponins from soy bean and mung bean inhibit the antigen specific activation of helper T cells by blocking cell cycle progression.
Treatment of helper T (Th) cells with saponins from soy bean and mung bean prevented their activation by inhibiting cell proliferation and cytokine secretion. However, the saponins did not affect the expression of major histocompatibility complex class II (A(b)) and co-stimulatory molecule (CD86) on professional antigen-presenting cells. Instead, the saponins directly inhibited Th cell proliferation by blocking the G(1) to S phase cell cycle transition. Moreover, blocking of the cell cycle by the saponins was achieved by decreased expression of cyclin D1 and cyclin E, and constitutive expression of p27(KIP1). Saponins also increased stability of p27(KIP1) in Th cells after antigenic stimulation.
Division of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
Suk Jun
SJ
Lee
Joonbeom
J
Bae
Sunhee
S
Kim
Seonah
S
Jeong
Chang-Yong
CY
Choi
Sang-Pil
SP
Choi
Hyun-Sook
HS
Kim
Woon-Won
WW
Jung
Jee-Young
JY
Imm
Sae Hun
SH
Kim
Taehoon
T
Chun
D000941:Antigens / Q000276:immunology*; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D016207:Cytokines / Q000378:metabolism; D007887:Fabaceae / Q000737:chemistry; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D012503:Saponins / Q000302:isolation & purification / Q000378:metabolism*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000502:physiology*
D000941:Antigens; D016207:Cytokines; D006131:Growth Inhibitors; D012503:Saponins