Nonstructural protein-1 of respiratory syncytial virus regulates HOX gene expression through interacting with histone.
Human respiratory syncytial virus (RSV), a major cause of severe respiratory diseases, constitutes an important risk factor for the development of subsequent asthma. In searching for its mechanism, the present study was designed to screen the interacting proteins of two important nonstructural (NS) proteins in human BECs. The subcellular localization and the effects of NS on HOX gene expression were also examined. The results showed that NS1 was distributed throughout the nucleus and cytoplasm, while NS2 was mainly distributed in cytoplasm of BECs. NS1 interacted specifically with host histone H2BD, inducing histone ubiquitination and subsequent HOX gene expression. In conclusion, the results of the present study indicated that RSV NS-1 induces HOX gene expression, through histone ubiquitination in a BEC cell line, which may provide a novel conception for understanding the relationship between severe RSV bronchiolitis during early life and the development of subsequent asthma.
Department of Basic Medical, Xiangya School of Medicine, Central South University, Changsha 410078, Hunan, China.
Yu-Rong
YR
Tan
Dan
D
Peng
Cui-Mei
CM
Chen
Xiao-Qun
XQ
Qin
D002352:Carrier Proteins; D002460:Cell Line; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping; D021381:Protein Transport; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
D002352:Carrier Proteins; D006657:Histones; D018398:Homeodomain Proteins; D017361:Viral Nonstructural Proteins