Hybrid scaffolds built from PET and collagen as a model for vascular graft architecture.
A hybrid material with excellent mechanical and biological properties is produced by electrospinning a co-solution of PET and collagen. The fibers are mapped using SEM, confocal Raman microscopy and collagenase digestion assays. Fibers of different compositions and morphologies are intermingled within the same membrane, resulting in a heterogeneous scaffold. The collagen distribution and exposure are found to depend on the PET/collagen ratio. The materials are chemically and mechanically characterized and biologically tested with fibroblasts (3T3-L1) and a HUVEC culture in vitro. All of the hybrid scaffolds show better cell attachment and proliferation than PET. These materials are potential candidates to be used as vascular grafts.
Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, 05508-900 São Paulo, Brazil.
Mariana C
MC
Burrows
Vitor M
VM
Zamarion
Fabíola B
FB
Filippin-Monteiro
Desirèe C
DC
Schuck
Henrique E
HE
Toma
Ana
A
Campa
Célia R S
CR
Garcia
Luiz H
LH
Catalani
D016475:3T3 Cells; D000818:Animals; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D003094:Collagen / Q000737:chemistry*; D017364:Collagenases / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007211:Indoles; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011092:Polyethylene Glycols / Q000737:chemistry*; D011093:Polyethylene Terephthalates; D013718:Tensile Strength; D054457:Tissue Scaffolds / Q000737:chemistry*
D007211:Indoles; D011093:Polyethylene Terephthalates; D011092:Polyethylene Glycols; C007293:DAPI; D003094:Collagen; D017364:Collagenases