A ligation-triggered highly sensitive fluorescent assay of adenosine triphosphate based on graphene oxide.
A simple, amplification-free and sensitive fluorescent biosensor for ATP detection was developed based on the ATP-dependent enzymatic reaction (ATP-DER) and the different adsorption affinity between graphene oxide (GO) and DNA structures. The proposed method was simple and convenient and also showed high sensitivity and selectivity to ATP.
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Wenping
W
Zhu
Ziwei
Z
Zhao
Zhen
Z
Li
Jianhui
J
Jiang
Guoli
G
Shen
Ruqin
R
Yu
D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry; D011088:DNA Ligases / Q000378:metabolism; D005452:Fluoresceins; D006108:Graphite / Q000737:chemistry; D012680:Sensitivity and Specificity
D052157:Aptamers, Nucleotide; D005452:Fluoresceins; C024098:6-carboxyfluorescein; D006108:Graphite; D000255:Adenosine Triphosphate; D004247:DNA; D011088:DNA Ligases