Single nucleotide polymorphisms of NR3C1 gene and recurrent depressive disorder in population of Poland.
Depressive disorder is a disease characterized by disturbances in the hypothalamo-pituitary-adrenal axis. Abnormalities include the increased level of glucocorticoids (GC) and changes in sensitivity to these hormones. The changes are related to glucocorticoid receptors gene (NR3C1) variants. The NR3C1 gene is suggested to be a candidate gene affecting depressive disorder risk and management. The aim of this study was to investigate polymorphisms within the NR3C1 gene and their role in the susceptibility to recurrent depressive disorder (rDD). 181 depressive patients and 149 healthy ethnically matched controls were included in the study. Single nucleotide polymorphisms were assessed using polymerase chain reaction/restriction fragment length polymorphism method. Statistical significance between rDD patients and controls was observed for the allele and genotype frequencies at three loci: BclI, N363S, and ER22/23EK. The presence of C allele, CC, and GC genotype of BclI polymorphism, G allele and GA genotype for N363S and ER22/23EK variants respectively were associated with increased rDD risk. Two haplotypes indicated higher susceptibility for rDD, while haplotype GAG played a protective role with OR(dis) 0.29 [95 % confidence interval (CI) = 0.13-0.64]. Data generated from this study support the earlier results that genetic variants of the NR3C1 gene are associated with rDD and suggest further consideration on the possible involvement of these variants in etiology of the disease.
Department of Endocrinology and Metabolic Diseases, Medical University of Łódź, 281/289, Rzgowska St, 93-338, Lodz, Poland.
Elżbieta
E
Gałecka
Janusz
J
Szemraj
Małgorzata
M
Bieńkiewicz
Ireneusz
I
Majsterek
Karolina
K
Przybyłowska-Sygut
Piotr
P
Gałecki
Andrzej
A
Lewiński
D000328:Adult; D054458:Amplified Fragment Length Polymorphism Analysis; D016022:Case-Control Studies; D003866:Depressive Disorder / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D011044:Poland; D020641:Polymorphism, Single Nucleotide; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D012008:Recurrence; D012306:Risk
C497713:NR3C1 protein, human; D011965:Receptors, Glucocorticoid