Extensimonas vulgaris gen. nov., sp. nov., a member of the family Comamonadaceae.
A novel strain, named S4(T), was obtained from industrial wastewater in Xiaoshan, Zhejiang Province, China. Cells were Gram-negative, neutrophilic and non-spore-forming and moved by means of a polar flagellum. Normal cells were 0.8-0.9 × 1.3-1.9 µm and the cells elongated to 10-25 µm when cultivated at high temperatures. Strain S4(T) grew at 15-50 °C (optimum at 48 °C), pH 5.5-8.5 (optimum 7.0-7.5) and 0-2% (optimum 0.5%) (w/v) NaCl. Ubiquinone-8 was the predominant respiratory quinone. C16:0, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH) and C17:0 cyclo were the major cellular fatty acids. The major 3-OH fatty acid was C10:0 3-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown aminoglycolipid. The genomic DNA G+C content was 68.8 mol%. Based on 16S rRNA gene sequences alignment, the most closely related strains were members of the genera Comamonas (94.6-95.6% similarities), Giesbergeria (94.9-95.6%), Acidovorax (94.8-95.4%), Brachymonas (94.1-95.2%) and Macromonas (95.1%). Phylogenetic analysis showed the closest relatives of strain S4(T) were members of the genus Macromonas. Based on phenotypic and phylogenetic characteristics, we suggest that strain S4(T) represents a novel species of a new genus of the family Comamonadaceae, for which the name Extensimonas vulgaris gen. nov., sp. nov. is proposed. The type strain of Extensimonas vulgaris is S4(T) (=CGMCC 1.10977(T)=JCM 17803(T)).
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Wei-Yan
WY
Zhang
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Ming-Xu
MX
Fang
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Wen-Wu
WW
Zhang
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Chuan
C
Xiao
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Xin-Qi
XQ
Zhang
Environmental Science Research & Design Institute of Zhejiang Province, Hangzhou 310007, PR China.
Zhi-Ping
ZP
Yu
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Xu-Fen
XF
Zhu
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Min
M
Wu
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis; D062065:Wastewater / Q000382:microbiology*; D014871:Water Microbiology
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; D062065:Wastewater; C030778:ubiquinone 8