Mannose7 glycan isomer characterization by IMS-MS/MS analysis.
The isomers of the Man(7)GlcNAc(2) glycan obtained from bovine ribonuclease B have been characterized by ion mobility spectrometry-tandem mass spectrometry (IMS-MS/MS). In these experiments, [Man7 + 2Na](2+) precursors having different mobilities are selected by ion mobility spectrometry and analyzed by MS/MS techniques in an ion trap. The fragmentation spectra obtained for various precursor ions are specific, suggesting the isolation or enrichment of different glycan isomers. One fragment ion with a mass-to-charge ratio (m/z) of 903.8 is found to correspond to the loss of an internal mannose residue of a specific isomer. Extracted fragment ion drift time distributions (XFIDTDs) yield distinctive precursor ion drift time profiles indicating the existence of four separate isomers as proposed previously.
Department of Chemistry, Indiana University, 800 Kirkwood Ave., Bloomington, IN 47405, USA.
Feifei
F
Zhu
Sunyoung
S
Lee
Stephen J
SJ
Valentine
James P
JP
Reilly
David E
DE
Clemmer
D000818:Animals; D002236:Carbohydrate Conformation; D002417:Cattle; D004867:Equipment Design; D007477:Ions / Q000737:chemistry; D007536:Isomerism; D008358:Mannose / Q000737:chemistry*; D008958:Models, Molecular; D011134:Polysaccharides / Q000737:chemistry*; D012260:Ribonucleases / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
D007477:Ions; D011134:Polysaccharides; D012260:Ribonucleases; C029600:ribonuclease B; D008358:Mannose