Spectroscopic investigation on the intermolecular interaction between N-confused porphyrins-(3-methylisoxazole) diad and bovine serum albumin.
The intermolecular interaction between N-confused porphyrins-(3-methylisoxazole) diad (NCP-(3-methylisoxazole)) and bovine serum albumin (BSA) has been investigated through fluorescence and ultraviolet spectroscopy at different temperatures under imitated physiological conditions. The results showed that the fluorescence of BSA was quenched by NCP-(3-methylisoxazole) through a combined quenching procedure. The characteristics of NCP-(3-methylisoxazole)⋯BSA interaction (including interaction nature, interaction conformation, binding constants, binding sites, binding distance, thermodynamic parameters, etc.) and the effect of metal ions (Cu(2+), Mg(2+), Ca(2+), and Ni(2+)) upon the NCP-(3-methylisoxazole)...BSA interaction have been detailed studied.
Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, Hunan Province College Key Laboratory of QSAR/QSPR, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.
Shiyu
S
Lu
Xianyong
X
Yu
Ying
Y
Yang
Xiaofang
X
Li
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D007555:Isoxazoles / Q000737:chemistry / Q000378:metabolism*; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics
D007555:Isoxazoles; D011166:Porphyrins; D012710:Serum Albumin, Bovine