Detection of transgenerational barium dual-isotope marks in salmon otoliths by means of LA-ICP-MS.
The present study evaluates the use of an individual-specific transgenerational barium dual-isotope procedure and its application to salmon specimens from the Sella River (Asturias, Spain). For such a purpose, the use of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in combination with multiple linear regression for the determination of the isotopic mark in the otoliths of the specimens is presented. In this sense, a solution in which two barium-enriched isotopes ((137)Ba and (135)Ba) were mixed at a molar ratio of ca. 1:3 (N Ba137/N Ba135) was administered to eight returning females caught during the spawning period. After injection, these females, as well as their offspring, were reared in a governmental hatchery located in the council of Cangas de Onís (Asturias, Spain). For comparison purposes, as well as for a time-monitoring control, egg and larva data obtained by solution analysis ICP-MS are also given. Otoliths (9-month-old juveniles) of marked offspring were analysed by LA-ICP-MS demonstrating a 100 % marking efficacy of this methodology. The capabilities of the molar fraction approach for 2D imaging of fish otoliths are also addressed.
Department of Physical and Analytical Chemistry, University of Oviedo, Oviedo, Spain.
Gonzalo
G
Huelga-Suarez
Beatriz
B
Fernández
Mariella
M
Moldovan
J Ignacio
JI
García Alonso
D000818:Animals; D001464:Barium / Q000008:administration & dosage / Q000032:analysis*; D005260:Female; D007554:Isotopes / Q000008:administration & dosage / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D010037:Otolithic Membrane / Q000737:chemistry*; D012474:Salmon / Q000033:anatomy & histology / Q000254:growth & development* / Q000378:metabolism
D007554:Isotopes; D001464:Barium