Cloning and functional verification of the Candida milleri HIS3 gene encoding imidazoleglycerol phosphate dehydratase.
The entire nucleotide sequence of the HIS3 gene encoding imidazoleglycerol phosphate dehydratase (IGPD) in yeast Candida milleri CBS 8195 was determined. Sequence analysis revealed an open-reading frame of C. milleri HIS3 that spans 678 bp, encodes 226 amino acids, and shares 80.5% amino acid identity to Torulaspora delbrueckii IGPD, followed by that to Saccharomyces cerevisiae (79.6%). The cloned HIS3 gene complemented a his3 mutation in S. cerevisiae, suggesting that it encodes a functional IGPD in C. milleri CBS 8195. A new auxotrophic marker is now available for acid-tolerant yeast C. milleri.
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea.
Eun-Hee
EH
Park
Se-Young
SY
Kwun
Seung-Ah
SA
Han
Jong-Sub
JS
Lee
Myoung-Dong
MD
Kim
D001483:Base Sequence; D002175:Candida / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004789:Enzyme Activation; D057075:Enzyme Assays; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006836:Hydro-Lyases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D017386:Sequence Homology, Amino Acid
D004271:DNA, Fungal; D005656:Fungal Proteins; D006836:Hydro-Lyases; C021434:imidazoleglycerolphosphate dehydratase