Differential morphological effects in rat corpora lutea among ethylene glycol monomethyl ether, atrazine, and bromocriptine.
Ethylene glycol monomethyl ether (EGME) or atrazine induces luteal cell hypertrophy in rats. Our previous study suggested that EGME stimulates both new and old corpora lutea (CL), while atrazine stimulates new CL. Bromocriptine (BRC) is known to suppress the luteolysis in rats. This study investigated the light- and electron-microscopic luteal changes induced by EGME, atrazine, or BRC. Female rats were treated with EGME (300 mg/kg/day), BRC (2 mg/kg/day), EGME and BRC (EGME + BRC), or atrazine (300 mg/kg/day) for 7 days. Luteal cell hypertrophy induced by EGME, EGME + BRC, and atrazine was subclassified into the following two types: CL hypertrophy, vacuolated type (CL-V) characterized by intracytoplasmic fine vacuoles, and CL hypertrophy, eosinophilic type (CL-E) characterized by eosinophilic and abundant cytoplasm. The proportions of CL-V and CL-E were different among the treatments. BRC-treated old CL showed lower proportion of endothelial cells and fibroblasts than normal old CL. Ultrastructural observation revealed that the luteal cells of CL-V contained abundant lipid droplets, whereas those of CL-E in EGME and EGME + BRC groups showed uniformly well-developed smooth endoplasmic reticulum. No clear ultrastructural difference was observed between the control CL and atrazine-treated CL-E. These results indicate that EGME, atrazine, and BRC have differential luteal morphological effects.
Division of Pathology, National Institute of Health Sciences, Tokyo 156-8501, Japan. y-taketa@hhc.eisai.co.jp
Yoshikazu
Y
Taketa
Kaoru
K
Inoue
Miwa
M
Takahashi
Jyoji
J
Yamate
Midori
M
Yoshida
D000818:Animals; D001280:Atrazine / Q000494:pharmacology*; D001971:Bromocriptine / Q000494:pharmacology*; D003338:Corpus Luteum / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D005026:Ethylene Glycols / Q000494:pharmacology*; D005260:Female; D006984:Hypertrophy; D008854:Microscopy, Electron; D051381:Rats; D017207:Rats, Sprague-Dawley
D005026:Ethylene Glycols; D001971:Bromocriptine; C005219:methyl cellosolve; D001280:Atrazine