Clinical and microbiological changes after minimally invasive therapeutic approaches in intrabony defects: a 12-month follow-up.
OBJECTIVE
This 12-month randomized, controlled trial evaluated the clinical effects and microbiological changes of minimally invasive nonsurgical and surgical approaches for the therapy of intrabony defects.


METHODS
Twenty-nine subjects with intrabony defects in single-rooted tooth were randomly assigned to; (1) minimally invasive nonsurgical technique (MINST) or (2) minimally invasive surgical technique (MIST). Quantities of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, and Porphyromonas gingivalis, determined by using real-time PCR, were evaluated at baseline, 3, 6, and 12 months after the treatments. Clinical recordings--probing depth (PD), position of the gingival margin (PGM), and relative clinical attachment level (RCAL)--were obtained at baseline and 12 months post-therapy. The primary outcome variable of the study was RCAL.


RESULTS
Both treatment modalities resulted in an improvement in all clinical recordings, with significant PD reductions (p < 0.05), RCAL gains (p < 0.05), and no change in the PGM (p > 0.05) after 12 months in both MINST and MIST groups. No clinical differences were observed between groups (p > 0.05). Regarding the microbiological outcomes, at the re-examinations, a significant decrease was observed for T. forsythia and P. gingivalis when compared with baseline (p < 0.05) for both treatments. The amount of A. actinomycetemcomitans did not reduced decrease throughout the study (p > 0.05). Intergroup differences in the microbiological assay were not found at any time point (p > 0.05).


CONCLUSIONS
Both MINST and MIST provided comparable clinical results and microbiological changes in the treatment of intrabony defects over 12 months follow-up.


CONCLUSIONS
This randomized, controlled, parallel trial revealed that both therapeutic modalities may promote clinical and microbiological benefits at 12 months post-therapy.
Dental Research Division, School of Dentistry, Paulista University, São Paulo, São Paulo, Brazil.
Fernanda V
FV
Ribeiro
Renato C V
RC
Casarin
Maria A G
MA
Palma
Francisco H N
FH
Júnior
Enilson A
EA
Sallum
Márcio Z
MZ
Casati
D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D016301:Alveolar Bone Loss / Q000382:microbiology / Q000628:therapy*; D001439:Bacteroides / Q000302:isolation & purification; D018441:Biofilms; D055113:Chronic Periodontitis / Q000382:microbiology / Q000628:therapy*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019060:Minimally Invasive Surgical Procedures; D017622:Periodontal Attachment Loss / Q000382:microbiology / Q000628:therapy; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D016896:Treatment Outcome