[Determination of 16 kinds of organophosphorus pesticides in serum by using GC/MS/MS].
OBJECTIVE
To establish a method of determining the presence and concentration of 16 kinds of organophosphorus pesticides in serum by GC/MS/MS.


METHODS
The parent ions of each pesticide was detected by running GC/MS in a full scan mode, and the peak areas and the ratio of signal to noise of each product ion were compared by setting different CID voltages. The CID voltage for each parent ion was optimized and determined. Serum samples were solid-phase extracted by Oasis HLB and eluted with ethyl ether after being concentrated and made to constant volume with ethyl ether, and then were tested by GC/MS/MS.


RESULTS
The correlation coefficient for each compound in the range of 0 - 1000ng/ml was greater than 0. 990. Recoveries of standard addition were 75% - 129% ,RSD < 10%. The intra-day and inter-day RSD was less than 15% (n = 6). The limits of quantification were less than 2ng/ml except for dichlorovos (55ng/ml).


CONCLUSIONS
GC/MS/MS assay is sensitive and accurate for the determination of pesticides in serum.
Institute for Environmental Health and Related Product Safety, Chinese CDC, Beijing 100021, China. meijunzhou77@hotmail.com
Zhirong
Z
Zhou
Hui
H
Fu
Tian
T
Luo
Shaobin
S
Lin
D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010575:Pesticides / Q000097:blood*; D053719:Tandem Mass Spectrometry
D010575:Pesticides