Glycan array analysis of the antigen repertoire targeted by tumor-binding antibodies.
Immunization with whole cells has been used extensively to generate monoclonal antibodies, produce protective immune responses, and discover new disease antigens. While glycans are abundant on cell surfaces, anti-glycan immune responses have not been well-characterized. We used glycan microarrays to profile 49 tumor-binding monoclonal antibodies generated by immunizing mice with whole cancer cells. A substantial proportion (41%) of the tumor binding antibodies bound carbohydrate antigens. The antibodies primarily recognize a group of 5 glycan antigens: Sialyl Lewis A (SLeA), Lewis A (LeA), Lewis X (LeX), blood group A (BG-A), and blood group H on a type 2 chain (BG-H2). The results have important implications for monoclonal antibody production and cancer vaccine development.
Chemical Biology Laboratory, National Cancer Institute, Frederick, MD 21702, United States. gildersj@mail.nih.gov
Jeffrey C
JC
Gildersleeve
Baomei
B
Wang
Samuel
S
Achilefu
Zhude
Z
Tu
Mai
M
Xu
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000941:Antigens / Q000737:chemistry* / Q000276:immunology; D019496:Cancer Vaccines / Q000097:blood; D007983:Lewis Blood Group Antigens / Q000276:immunology; D051379:Mice; D046228:Microarray Analysis; D009369:Neoplasms / Q000276:immunology / Q000517:prevention & control; D011134:Polysaccharides / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000941:Antigens; D019496:Cancer Vaccines; D007983:Lewis Blood Group Antigens; D011134:Polysaccharides