Localisation of a family of complex-forming β-barrels in the T. vaginalis hydrogenosomal membrane.
Crucial to organellogenesis was the development of membrane translocases responsible for delivering proteins to new cellular compartments. This investigation examines the Trichomonas vaginalis hydrogenosome, a mitochondrially derived organelle. We identify an expanded family of putative β-barrel proteins (THOM A-I) comprising nine related sequences. Sub-cellular localisation by immunofluorescence and biochemical fractionation is consistent with THOMs being localised to the hydrogenosomal membrane. Native gel electrophoresis and chemical cross-linking support the ability of THOM proteins to be components of membrane-bound oligomeric protein complexes, consistent with a role in protein translocation.
School of Biomedical Sciences, University of Nottingham Medical School, Queen's Medical Centre, Nottingham, United Kingdom.
Christopher
C
Kay
Karen
K
Lawler
Tim J
TJ
Self
Sabrina D
SD
Dyall
Ian D
ID
Kerr
D015153:Blotting, Western; D047468:Immunoprecipitation; D007425:Intracellular Membranes / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D046912:Multiprotein Complexes / Q000378:metabolism; D015388:Organelles / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014246:Trichomonas vaginalis / Q000235:genetics / Q000378:metabolism*
D026901:Membrane Transport Proteins; D046912:Multiprotein Complexes; D015800:Protozoan Proteins