Application of thermophilic enzymes and water jet system to cassava pulp.
Co-production of fermentable sugars and nanofibrillated cellulose from cassava pulp was achieved by the combination of thermophilic enzymes (endoglucanase, β-glucosidase, and α-amylase) and a new atomization system (Star Burst System; SBS), which employs opposing water jets. The SBS represents a key technology for providing cellulose nanofibers and improving the enzymatic saccharification of cassava pulp. Depending on the enzymes used, the production of glucose from cassava pulp treated with the SBS was 1.2- to 2.5-fold higher than that from pulp not treated with the SBS. Nanofibrillated cellulose with the gel-like property in suspension was produced (yield was over 90%) by α-amylase treatment, which completely released trapped starch granules from the fibrous cell wall structure of cassava pulp pretreated with the SBS. The SBS provides an environmentally low-impact pretreatment system for processing biomass material into value-added products.
National Institute of Advanced Industrial Science and Technology, Biomass Refinery Research Center, 3-11-32 Kagamiyama, Higashi-hiroshima, Hiroshima 739-0046, Japan.
Siriporn
S
Chaikaew
Yuka
Y
Maeno
Wonnop
W
Visessanguan
Kota
K
Ogura
Gaku
G
Sugino
Seung-Hwan
SH
Lee
Kazuhiko
K
Ishikawa
D050260:Carbohydrate Metabolism; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D002365:Manihot / Q000378:metabolism*; D057139:Nanofibers / Q000648:ultrastructure; D013696:Temperature; D014867:Water / Q000737:chemistry*; D000516:alpha-Amylases / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D014867:Water; D002482:Cellulose; D000516:alpha-Amylases; D001617:beta-Glucosidase; D002480:Cellulase