Serum microRNA expression profile as a biomarker for the diagnosis of pertussis.
Pertussis is a highly contagious, respiratory disease associated with substantial morbidity and mortality. A rapid and reliable diagnostic method is essential for appropriate treatment and prevention. Expression profiles of circulating microRNAs (miRNAs) have been proven as new non-invasive biomarkers for infectious diseases. We aimed to investigated the serum miRNA profile in pertussis patients and explored its potential as a novel diagnostic biomarker for pertussis. Among 664 different miRNAs analyzed using a miRNA array, 50 were overexpressed and 81 were underexpressed in the serum of pertussis patients. Expression levels of seven candidate miRNAs were further evaluated by real-time qRT-PCR. A panel of five miRNAs (miR-202, miR-342-5p, miR-206, miR-487b, miR-576-5p) was confirmed overexpressed in pertussis patients (p < 0.05). Risk score and receiver-operating characteristic (ROC) analysis showed that the area under the curve of the five-member miRNA profile was 0.980. At an optimal cutoff value (0.707), this panel of miRNAs yielded a sensitivity of 97.4 % and a specificity of 94.3 %. These data suggest that the five-member serum miRNA profile may serve as a new biomarker for pertussis diagnosis with high specificity and sensitivity.
Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health, Nanjing, 210009, China.
Yiyue
Y
Ge
Kangchen
K
Zhao
Yuhua
Y
Qi
Xiaoyan
X
Min
Zhiyang
Z
Shi
Xian
X
Qi
Yunfeng
Y
Shan
Lan
L
Cui
Minghao
M
Zhou
Yong
Y
Wang
Hua
H
Wang
Lunbiao
L
Cui
D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007223:Infant; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012306:Risk; D059467:Transcriptome; D014917:Whooping Cough / Q000097:blood* / Q000175:diagnosis*
D015415:Biomarkers; C000590114:MIRN576 microRNA, human; D035683:MicroRNAs