Structure of the mediator head module bound to the carboxy-terminal domain of RNA polymerase II.
The X-ray crystal structure of the Head module, one-third of the Mediator of transcriptional regulation, has been determined as a complex with the C-terminal domain (CTD) of RNA polymerase II. The structure reveals multiple points of interaction with an extended conformation of the CTD; it suggests a basis for regulation by phosphorylation of the CTD. Biochemical studies show a requirement for Mediator-CTD interaction for transcription.
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Philip J J
PJ
Robinson
David A
DA
Bushnell
Michael J
MJ
Trnka
Alma L
AL
Burlingame
Roger D
RD
Kornberg
D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012319:RNA Polymerase II / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D053719:Tandem Mass Spectrometry
D012319:RNA Polymerase II