Fourier transform infrared imaging analysis in discrimination studies of St. John's wort (Hypericum perforatum).
In the present study, Fourier transform infrared (FTIR) imaging and data analysis methods were combined to study morphological and molecular patterns of St. John's wort (Hypericum perforatum) in detail. For interpretation, FTIR imaging results were correlated with histological information gained from light microscopy (LM). Additionally, we tested several evaluation processes and optimized the methodology for use of complex FTIR microscopic images to monitor molecular patterns. It is demonstrated that the combination of the used spectroscopic method with LM enables a more distinct picture, concerning morphology and distribution of active ingredients, to be gained. We were able to obtain high-quality FTIR microscopic imaging results and to distinguish different tissue types with their chemical ingredients.
Institute of Analytical Chemistry and Radiochemistry, CCB-Center for Chemistry and Biomedicine, Leopold-Franzens University, Innsbruck, Austria.
V A
VA
Huck-Pezzei
J D
JD
Pallua
C
C
Pezzei
L K
LK
Bittner
S A
SA
Schönbichler
G
G
Abel
M
M
Popp
G K
GK
Bonn
C W
CW
Huck
D016002:Discriminant Analysis; D020902:Hypericum / Q000033:anatomy & histology / Q000737:chemistry*; D011786:Quality Control; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*