Studies on reactivation of regressing bonnet monkey corpus luteum on day 1 of menses: a pilot study.
Studies on functional characteristics of the regressing primate corpus luteum (CL) to luteotrophic stimulus on day 1 of the non-fertile menstrual cycle are scarce. Recombinant human luteinizing hormone (rhLH) (20 IU/Kg BW; n = 10) or human chorionic gonadotropin (hCG) (180 IU; n = 6) were administered intravenously to female bonnet monkeys on day 1 of menses. Exogenous treatment of rhLH or hCG caused a significant increase in circulating progesterone (P(4)) levels 2-4 hours post treatment (P < 0.05). Lutectomy prior to onset of menses confirmed that CL is the site of the increased P(4) concentrations. Increased levels of phosphorylated P44/42 MAPK, MKK3/6 activation and concomitant histological changes were observed within 4 hours in CL of monkeys receiving hCG treatment. The results from this study demonstrate the acute progesterone synthesizing capacity of regressing monkey CL after LH or hCG challenge. This has potential implications for interpreting the steroidogenic response after gonadotropin stimulation tests in the early follicular phase of the normal ovulatory and anovulatory women undergoing controlled ovarian stimulation protocols as part of assisted reproductive technology (ART) and in women with polycystic ovarian syndrome.
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India.
Padmanaban S
PS
Suresh
Rudraiah
R
Medhamurthy
D000818:Animals; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D003338:Corpus Luteum / Q000502:physiology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D006801:Humans; D007986:Luteinizing Hormone / Q000494:pharmacology; D003341:Luteolysis / Q000187:drug effects / Q000502:physiology*; D008255:Macaca radiata / Q000502:physiology*; D008598:Menstruation; D010865:Pilot Projects; D011374:Progesterone / Q000378:metabolism
D006063:Chorionic Gonadotropin; D011374:Progesterone; D007986:Luteinizing Hormone; D048049:Extracellular Signal-Regulated MAP Kinases