A bioinformatics approach for integrated transcriptomic and proteomic comparative analyses of model and non-sequenced anopheline vectors of human malaria parasites.
Malaria morbidity and mortality caused by both Plasmodium falciparum and Plasmodium vivax extend well beyond the African continent, and although P. vivax causes between 80 and 300 million severe cases each year, vivax transmission remains poorly understood. Plasmodium parasites are transmitted by Anopheles mosquitoes, and the critical site of interaction between parasite and host is at the mosquito's luminal midgut brush border. Although the genome of the "model" African P. falciparum vector, Anopheles gambiae, has been sequenced, evolutionary divergence limits its utility as a reference across anophelines, especially non-sequenced P. vivax vectors such as Anopheles albimanus. Clearly, technologies and platforms that bridge this substantial scientific gap are required in order to provide public health scientists with key transcriptomic and proteomic information that could spur the development of novel interventions to combat this disease. To our knowledge, no approaches have been published that address this issue. To bolster our understanding of P. vivax-An. albimanus midgut interactions, we developed an integrated bioinformatic-hybrid RNA-Seq-LC-MS/MS approach involving An. albimanus transcriptome (15,764 contigs) and luminal midgut subproteome (9,445 proteins) assembly, which, when used with our custom Diptera protein database (685,078 sequences), facilitated a comparative proteomic analysis of the midgut brush borders of two important malaria vectors, An. gambiae and An. albimanus.
W Harry Feinstone Department of Molecular Microbiology & Immunology, Johns Hopkins Bloomberg School of Public Health & Malaria Research Institute, Baltimore, Maryland 21205, USA.
Ceereena
C
Ubaida Mohien
David R
DR
Colquhoun
Derrick K
DK
Mathias
John G
JG
Gibbons
Jennifer S
JS
Armistead
Maria C
MC
Rodriguez
Mario Henry
MH
Rodriguez
Nathan J
NJ
Edwards
Jürgen
J
Hartler
Gerhard G
GG
Thallinger
David R
DR
Graham
Jesus
J
Martinez-Barnetche
Antonis
A
Rokas
Rhoel R
RR
Dinglasan
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000235:genetics* / Q000469:parasitology; D002853:Chromatography, Liquid; D019295:Computational Biology; D030562:Databases, Protein; D006790:Host-Parasite Interactions; D006801:Humans; D019476:Insect Proteins / Q000032:analysis* / Q000737:chemistry; D007303:Insect Vectors / Q000235:genetics* / Q000469:parasitology; D008288:Malaria / Q000469:parasitology; D008871:Microvilli; D010963:Plasmodium falciparum; D010966:Plasmodium vivax; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D012313:RNA / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D059467:Transcriptome
D019476:Insect Proteins; D020543:Proteome; D012313:RNA