Transcriptome analysis of garlic-induced hepatoprotection against alcoholic fatty liver.
Fatty liver induced by alcohol abuse is a major worldwide health hazard leading to morbidity and mortality. Previous studies indicate antifatty liver properties of garlic. This study investigated the molecular mechanisms of garlic oil (GO) or diallyl disulfide (DADS) imparted hepatoprotection against alcohol induced fatty liver in C57BL/6 mice using microarray-based global gene expression analysis. Alcohol liquid diet resulted in severe fatty liver with increased levels of serum aspartate aminotransferease and alanine aminotransferease as well as triglycerides and decreased levels of liver glutathione and antioxidant enzymes. The major canonical pathways implicated by alcohol treatment are the metabolisms of xenobiotics by cytochrome P450, glutathione, and arachidonic acid. Treatment with DADS or GO normalized the serum aminotransferease levels and liver antioxidant enzymes and reduced the contents of triglycerides and cholesterol. The canonical pathways involved in the amelioration of liver include arachidonic acid metabolism, altered T cell and B cell signaling, tryptophan metabolism, antigen presentation pathway for DADS, metabolism of xenobiotics, mitotic roles of Polo-like kinase, fatty acid metabolism, LPS/IL-1 mediated inhibition of RXR function, and C21-steroid hormone metabolism for GO.
Institute of Food Science and Technology, National Taiwan University, Taipei 106, Taiwan.
Rajasekaran
R
Raghu
Chun-Ting
CT
Liu
Mong-Hsun
MH
Tsai
Xiaojia
X
Tang
Krishna R
KR
Kalari
Subbaya
S
Subramanian
Lee-Yan
LY
Sheen
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005235:Fatty Liver, Alcoholic / Q000235:genetics / Q000517:prevention & control*; D005737:Garlic; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
D017931:DNA Primers