Characterization of a butanol-acetone-producing Clostridium strain and identification of its solventogenic genes.
A unique Clostridium species strain G117 was obtained in this study to be capable of producing dominant butanol from glucose. Butanol of 13.50 g/L was produced when culture G117 was fed with 60 g/L glucose, which is ~20% higher than previously reported butanol production by wild-type Clostridium acetobutylicum ATCC 824 under similar conditions. Strain G117 also distinguishes itself by generating negligible amount of ethanol, but producing butanol and acetone as biosolvent end-products. A butanol dehydrogenase gene (bdh gene) was identified in strain G117, which demonstrated a ~200-fold increase in transcription level measured by quantitative real-time PCR after 10h of culture growth. The high transcription suggests that this bdh gene could be a putative gene involved in butanol production. In all, Clostridium sp. strain G117 serves as a potential candidate for industrial biobutanol production while the absence of ethanol ensures an economic-efficient separation and purification of butanol.
Department of Civil and Environmental Engineering, National University of Singapore, Singapore.
Teck Khiang
TK
Chua
Da-Wei
DW
Liang
Chao
C
Qi
Kun-Lin
KL
Yang
Jianzhong
J
He
D000096:Acetone / Q000378:metabolism*; D000426:Alcohol Dehydrogenase / Q000737:chemistry; D020816:Amino Acid Motifs; D001483:Base Sequence; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D005947:Glucose / Q000494:pharmacology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012997:Solvents / Q000378:metabolism*
D000440:Butanols; D005232:Fatty Acids, Volatile; D012336:RNA, Ribosomal, 16S; D012997:Solvents; D000096:Acetone; D000426:Alcohol Dehydrogenase; D005947:Glucose