Molecular mechanisms of fMLP-induced superoxide generation and degranulation in mouse neutrophils.
In this manuscript, involvement of PLD in fMLP-induced superoxide generation and degranulation were re-investigated using PLD(-/-) neutrophils, and the molecular mechanisms of these neutrophil functions were examined. Neither PLD1 nor PLD2 is involved in these fMLP-induced neutrophil functions. The results obtained in this study provide evidence that cPKC plays an important role in fMLP-induced superoxide generation. On the other hand, Ca(2+)-dependent signaling pathway and cPKC seem to be involved in degranulation.
Department of Physiological Chemistry, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tennodai, Ibaraki 305-8575, Japan. ykanaho@md.tsukuba.ac.jp
Yasunori
Y
Kanaho
Takanobu
T
Sato
Tsunaki
T
Hongu
Yuji
Y
Funakoshi
D000818:Animals; D002118:Calcium / Q000378:metabolism; D061207:Calcium Ionophores / Q000494:pharmacology; D015550:Cell Degranulation / Q000187:drug effects*; D004075:Diglycerides / Q000494:pharmacology; D051379:Mice; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D015398:Signal Transduction; D013481:Superoxides / Q000378:metabolism*
D061207:Calcium Ionophores; D004075:Diglycerides; D010713:Phosphatidylcholines; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; C038237:1-oleoyl-2-acetylglycerol; D011493:Protein Kinase C; C105606:phospholipase D2; D010739:Phospholipase D; C106801:phospholipase D1; D002118:Calcium