The serotype-independent but concentration-dependent enhancing antibodies among Thai dengue patients.
Antibody-dependent enhancement of infection (ADE) is central to explaining the development of severe disease at the end of post-dengue virus infection. Non-neutralizing anti-dengue antibodies bound to the dengue virion enhances the virus entrance into the target cells via the Fc receptor. The titer of enhancing antibodies in dengue patients is not determined during dengue virus infection. Sensitive flow cytometry detecting dengue virus-infected K562 cells was used to quantitate enhancing activity among Thai DF and DHF patients against four serotypes and the patient's dengue isolate. The titer was defined as the reciprocal of the final dilution that loses enhancing activity. The serum of Thai patients confirmed to have dengue infection were found to have high titers of enhancing antibodies and increased gradually through the convalescent phase of infection. The enhancing antibody titers were not different among the four serotypes or from the infecting isolate. The anti-dengue antibodies from dengue patients can enhance dengue virus infections in a concentration-dependent, serotype-independent manner.
Si Sa Ket Provincial Hospital, Office of Permanent Secretary, Ministry of Public Health, Si Sa Ket, Thailand.
Chai
C
Teerasut
Huan-Yao
HY
Lei
Khuendee
K
Natthakorn
Akanitt
A
Jittmittraphap
Suwich
S
Thammapalo
Prasong
P
Witayathawornwong
Ya-Tin
YT
Lin
Richard G
RG
Jarman
Pathom
P
Sawanpanyalert
Wipawee
W
Jampangern
Kriengsak
K
Limkittikul
D019138:Antibodies, Blocking / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D019067:Antibody-Dependent Enhancement / Q000276:immunology*; D003715:Dengue / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology* / Q000821:virology; D005434:Flow Cytometry; D006801:Humans; D020014:K562 Cells; D011961:Receptors, Fc / Q000276:immunology; D012703:Serotyping; D019595:Severe Dengue / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology / Q000821:virology; D013785:Thailand / Q000453:epidemiology
D019138:Antibodies, Blocking; D000914:Antibodies, Viral; D011961:Receptors, Fc