Salivary type I collagen degradation end-products and related matrix metalloproteinases in periodontitis.
OBJECTIVE
Type I collagen degradation end-products and related matrix metalloproteinases (MMPs) were examined aiming to detect potential markers of periodontitis in saliva, with high sensitivity and specificity.


METHODS
The salivary concentrations of MMP-8, MMP-9 and MMP-13, tartrate-resistant acid phosphatase serum type 5b, C-terminal cross-linked telopeptide of type I collagen (CTx), N-terminal cross-linked telopeptide of type I collagen (NTx) and cross-linked carboxyterminal telopeptide of type I collagen were analysed in 230 subjects. Oral health examination included panoramic radiography.


RESULTS
The concentrations of MMP-8, MMP-9 and MMP-13 in saliva were higher in subjects with generalized periodontitis than in controls. Of the tested salivary markers, MMP-8 was the only marker capable of differentiating subjects with severe alveolar bone loss from those with slight bone loss (p < 0.001). The association between the salivary MMP-8 levels and periodontitis remained significant after the adjustment with age, gender and smoking. In addition, significant correlations were found between the tested markers and periodontal parameters.


CONCLUSIONS
Enzymes and end-products of type I collagen degradation have different associations with each other and with periodontal status that may reflect their roles in the cascade leading to alveolar bone loss. MMP-8 is a strong biomarker candidate for detecting alveolar bone destruction.
Institute of Dentistry, University of Turku, Turku, Finland. ulvi.gursoy@utu.fi
Ulvi K
UK
Gursoy
Eija
E
Könönen
Sisko
S
Huumonen
Taina
T
Tervahartiala
Pirkko J
PJ
Pussinen
Anna L
AL
Suominen
Timo
T
Sorsa
D000135:Acid Phosphatase / Q000378:metabolism; D010520:Aggressive Periodontitis / Q000175:diagnosis / Q000378:metabolism*; D016301:Alveolar Bone Loss / Q000378:metabolism*; D000704:Analysis of Variance; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000175:diagnosis / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D016015:Logistic Models; D008297:Male; D053509:Matrix Metalloproteinase 13 / Q000378:metabolism; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008875:Middle Aged; D010455:Peptides / Q000378:metabolism; D062106:Protein Degradation End Products / Q000378:metabolism*; D012463:Saliva / Q000737:chemistry; D012680:Sensitivity and Specificity; D018709:Statistics, Nonparametric; D000071681:Tartrate-Resistant Acid Phosphatase
D015415:Biomarkers; D024042:Collagen Type I; D007527:Isoenzymes; D010455:Peptides; D062106:Protein Degradation End Products; C052929:collagen type I trimeric cross-linked peptide; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D053509:Matrix Metalloproteinase 13; D020784:Matrix Metalloproteinase 8; D020780:Matrix Metalloproteinase 9