Multi-copy genetic screen in Aspergillus nidulans.
With the completion of genomes of various Aspergillus species, large-scale genome-wide expression studies can be carried out. Genomics, however, is more powerful and efficient when combined with genetics. A multi-copy-based gain-of-function screen is a complementary method to loss-of-function genetic screen and can identify novel genes that may not be easily identifiable through loss-of-function-type screens. Particularly, gain-of-function genetic screens would identify novel activators or repressors of fungal development and secondary metabolism. Here, we describe a working protocol for the identification of novel regulators in Aspergillus nidulans.
Molecular and Environmental Toxicology Center, University of Wisconsin-Madison, Madison, WI, USA.
Hee Soo
HS
Park
Jae-Hyuk
JH
Yu
D001233:Aspergillus nidulans / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D005821:Genetic Techniques; D016681:Genome, Fungal / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA
D004271:DNA, Fungal