A resource for manipulating gene expression and analyzing cis-regulatory modules in the Drosophila CNS.
Here, we describe the embryonic central nervous system expression of 5,000 GAL4 lines made using molecularly defined cis-regulatory DNA inserted into a single attP genomic location. We document and annotate the patterns in early embryos when neurogenesis is at its peak, and in older embryos where there is maximal neuronal diversity and the first neural circuits are established. We note expression in other tissues, such as the lateral body wall (muscle, sensory neurons, and trachea) and viscera. Companion papers report on the adult brain and larval imaginal discs, and the integrated data sets are available online (http://www.janelia.org/gal4-gen1). This collection of embryonically expressed GAL4 lines will be valuable for determining neuronal morphology and function. The 1,862 lines expressed in small subsets of neurons (<20/segment) will be especially valuable for characterizing interneuronal diversity and function, because although interneurons comprise the majority of all central nervous system neurons, their gene expression profile and function remain virtually unexplored.
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA.
Laurina
L
Manning
Ellie S
ES
Heckscher
Maria D
MD
Purice
Jourdain
J
Roberts
Alysha L
AL
Bennett
Jason R
JR
Kroll
Jill L
JL
Pollard
Marie E
ME
Strader
Josh R
JR
Lupton
Anna V
AV
Dyukareva
Phuong Nam
PN
Doan
David M
DM
Bauer
Allison N
AN
Wilbur
Stephanie
S
Tanner
Jimmy J
JJ
Kelly
Sen-Lin
SL
Lai
Khoa D
KD
Tran
Minoree
M
Kohwi
Todd R
TR
Laverty
Joseph C
JC
Pearson
Stephen T
ST
Crews
Gerald M
GM
Rubin
Chris Q
CQ
Doe
D000818:Animals; D030801:Animals, Genetically Modified; D002490:Central Nervous System / Q000254:growth & development / Q000378:metabolism*; D016208:Databases, Factual; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D020407:Internet; D008297:Male; D050436:Regulatory Elements, Transcriptional; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D014157:Transcription Factors