Design and fabrication of a COP-based microfluidic chip: chronoamperometric detection of Troponin T.
This work demonstrates the design and fabrication of an all cyclo-olefin polymer based microfluidic device capable of capturing magnetic beads and performing electrochemical detection in a series of gold electrodes. The size of chip is of a microscope slide and features six independent measuring cells for multianalyte detection purposes. The aim of this work is to show that rapid prototyping techniques can be instrumental in the development of novel bioassays, particularly in clinical diagnosis applications. We show the successful determination of troponin-T, a cardiac disease marker, in the clinically relevant range of 0.05-1.0 ng/mL. This methodology achieves a detection limit of 0.017 ng/mL in PBS solutions, and is capable of detecting less than 1 ng/mL in a 1:50 human serum dilution.
Instituto de Microelectrónica de Barcelona, IMB-CNM-CSIC, Universitat Autónoma de Barcelona, Barcelona, Spain.
Llibertat
L
Abad
Francisco
F
Javier del Campo
Francesc Xavier
FX
Muñoz
Luis J
LJ
Fernández
Daniel
D
Calavia
Gloria
G
Colom
Juan P
JP
Salvador
María Pilar
MP
Marco
Vanessa
V
Escamilla-Gómez
Berta
B
Esteban-Fernández de Ávila
Susana
S
Campuzano
María
M
Pedrero
José M
JM
Pingarrón
Neus
N
Godino
Robert
R
Gorkin
Jens
J
Ducrée
D055910:Antibodies, Immobilized / Q000737:chemistry / Q000378:metabolism; D002138:Calibration; D003516:Cycloparaffins / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation / Q000379:methods; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D011108:Polymers / Q000737:chemistry; D020107:Troponin T / Q000032:analysis* / Q000097:blood*
D055910:Antibodies, Immobilized; D003516:Cycloparaffins; D011108:Polymers; D020107:Troponin T