Prevalence of genomic island PAPI-1 in clinical isolates of Pseudomonas aeruginosa in Iran.
Pseudomonas aeruginosa, a gram-negative rod-shaped bacterium, is an opportunistic pathogen, which causes various serious diseases in humans and animals. The aims of this study were to evaluate of the presence of genomic island PAPI-1 in Pseudomonas aeruginosa isolated from Reference Laboratory of Ilam, Milad Hospital and Emam Khomeini Hospital, Iran and to study the frequency of extended spectrum beta-lactamases (ESBLs) among isolates. Forty-eight clinical isolates of P. aeruginosa were obtained during April to September 2010, and were evaluated for ESBLs by screening and confirmatory disk diffusion methods and PAPI-1 by PCR. Fifteen of 48 P. aeruginosa isolates were positive for ESBLs and 17 isolates positive for PAPI-1. This was first study of the prevalence of PAPI-1 in clinical isolates of P. aeruginosa in Iran, showing that most of PAPI-1 positive strains had high levels of antibiotic resistance and produced ESBLs.
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Nourkhoda
N
Sadeghifard
Seyedeh Zahra
SZ
Rasaei
Sobhan
S
Ghafourian
Mohammad Reza
MR
Zolfaghary
Reza
R
Ranjbar
Mohammad
M
Raftari
Reza
R
Mohebi
Abbas
A
Maleki
Mohammad
M
Rahbar
D002876:Chromosomes, Bacterial / Q000235:genetics; D044404:Genomic Islands / Q000235:genetics*; D006801:Humans; D007492:Iran; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011552:Pseudomonas Infections / Q000235:genetics / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics*