Molecular cloning, sequencing, and expression analysis of cDNA encoding metalloprotein II (MP II) induced by single and combined metals (Cu(II), Cd(II)) in polychaeta Perinereis aibuhitensis.
We amplified and analyzed the complete cDNA of metalloprotein II (MP II) from the somatic muscle of the polychaete Perinereis aibuhitensis, the full length cDNA is 904 bp encoding 119 amino acids. The MP II cDNA sequence was subjected to BLAST searching in NCBI and was found to share high homology with hemerythrin of other worms. MP II expression of P. aibuhitensis exposed to single and combined metals (Cu(II), Cd(II)) was analyzed using real time-PCR. MP II mRNA expression increased at the start of Cu(II) exposure, then decreased and finally return to the normal level. Expression pattern of MP II under Cd(II) exposure was time- and dose-dependent. MP II expression induced by a combination of Cd(II) and Cu(II) was similar to that induced by Cd(II) alone.
Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Key Laboratory of Mariculture and Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, PR China.
Dazuo
D
Yang
Yibing
Y
Zhou
Huan
H
Zhao
Xiaoxiao
X
Zhou
Na
N
Sun
Bin
B
Wang
Xiutang
X
Yuan
D000595:Amino Acid Sequence; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D003001:Cloning, Molecular; D003300:Copper / Q000633:toxicity*; D018076:DNA, Complementary / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression; D008667:Metalloproteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011077:Polychaeta; D012333:RNA, Messenger / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D018076:DNA, Complementary; D008667:Metalloproteins; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D002104:Cadmium; D003300:Copper