Recovery of diverse microbes in high turbidity surface water samples using dead-end ultrafiltration.
Dead-end ultrafiltration (DEUF) has been reported to be a simple, field-deployable technique for recovering bacteria, viruses, and parasites from large-volume water samples for water quality testing and waterborne disease investigations. While DEUF has been reported for application to water samples having relatively low turbidity, little information is available regarding recovery efficiencies for this technique when applied to sampling turbid water samples such as those commonly found in lakes and rivers. This study evaluated the effectiveness of a DEUF technique for recovering MS2 bacteriophage, enterococci, Escherichia coli, Clostridium perfringens, and Cryptosporidium parvum oocysts in surface water samples having elevated turbidity. Average recovery efficiencies for each study microbe across all turbidity ranges were: MS2 (66%), C. parvum (49%), enterococci (85%), E. coli (81%), and C. perfringens (63%). The recovery efficiencies for MS2 and C. perfringens exhibited an inversely proportional relationship with turbidity, however no significant differences in recovery were observed for C. parvum, enterococci, or E. coli. Although ultrafilter clogging was observed, the DEUF method was able to process 100-L surface water samples at each turbidity level within 60 min. This study supports the use of the DEUF method for recovering a wide array of microbes in large-volume surface water samples having medium to high turbidity.
Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases, Division of Foodborne, Waterborne and Environmental Diseases, Atlanta, GA 30329-4018, USA. BMull@cdc.gov
Bonnie
B
Mull
Vincent R
VR
Hill
D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D044822:Biodiversity; D005618:Fresh Water / Q000032:analysis / Q000382:microbiology* / Q000469:parasitology*; D010271:Parasites / Q000737:chemistry / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000379:methods*; D014780:Viruses / Q000145:classification / Q000302:isolation & purification*; D014871:Water Microbiology