Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta).
BACKGROUND
Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior.


METHODS
We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data.


RESULTS
Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective.


CONCLUSIONS
Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.
Department of Population Health and Reproduction, University of California-Davis, Davis, CA, USA. gmkarere@txbiomedgenetics.org
Genesio M
GM
Karere
Erin
E
Sullivan
Erin L
EL
Kinnally
John P
JP
Capitanio
Leslie A
LA
Lyons
D000818:Animals; D001522:Behavior, Animal; D004247:DNA / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000662:veterinary; D005260:Female; D060005:Genotyping Techniques / Q000379:methods / Q000662:veterinary*; D008253:Macaca mulatta / Q000235:genetics*; D008297:Male; D008995:Monoamine Oxidase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011110:Polymorphism, Genetic / Q000235:genetics*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000235:genetics*
D050486:Serotonin Plasma Membrane Transport Proteins; D004247:DNA; D008995:Monoamine Oxidase