Allele-specific PCR for determination of IL28B genotype.
The IL28B genotype is a critical determinant of interferon response in patients infected with hepatitis C virus genotype 1. We describe an allele-specific PCR assay for the IL28B genotype. The assay is simple and robust, uses commonly available real-time PCR instrumentation, and is well suited for clinical laboratories offering IL28B genotyping.
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA.
Linda
L
Cook
Kurt
K
Diem
Woo
W
Kim
John D
JD
Scott
Keith R
KR
Jerome
D000483:Alleles; D005838:Genotype; D016174:Hepacivirus / Q000276:immunology; D006526:Hepatitis C / Q000188:drug therapy; D006801:Humans; D007372:Interferons; D007378:Interleukins / Q000235:genetics* / Q000627:therapeutic use; D016133:Polymerase Chain Reaction / Q000379:methods*; D000095022:Interferon Lambda
D007372:Interferons; D007378:Interleukins; C000624353:interferon-lambda, human; D000095022:Interferon Lambda