Characterization of yew tree (Taxus) varieties by fingerprint and principal component analyses.
The composition of varieties of Taxus growing in Estonia was analyzed by capillary electrophoresis with diode array detection (CE-DAD) for the separation of phenolic compounds, and by high performance liquid chromatography-mass spectrometry (HPLC-MS) for the determination of taxoids. The main purpose of this study was the chemotaxonomic differentiation of varieties of Taxus by using data from these analyses. Fingerprints scanned at 214 nm on the basis of CE separation at pH 9.3 were used to characterize seven varieties of yew. The contents of four key taxoids (10-deacetylbaccatin, baccatin III, cephalomannine and paclitaxel) in six Taxus varieties were comparatively determined by HPLC-MS. The set of electropherograms/chromatograms of the various Taxus extracts were subjected to principal component analysis (PCA), using the peak areas of 16 phenolic compounds and 14 taxoids as characteristics. The formation of distinct clusters in accordance with botanical classification proves the suitability of PCA for differentiating varieties of Taxus.
Department of Natural Sciences, Institute of Mathematics and Natural Sciences, Tallinn University, Narva mnt 25, 10120 Tallinn, Estonia.
Kalle
K
Truus
Merike
M
Vaher
Maria
M
Borissova
Marju
M
Robal
Tuuli
T
Levandi
Rando
R
Tuvikene
Peeter
P
Toomike
Mihkel
M
Kaljurand
D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D013058:Mass Spectrometry; D025341:Principal Component Analysis; D020946:Taxus / Q000737:chemistry*