Discriminating experimental Listeria monocytogenes infections in mice using serum profiling.
Serum profiling was used to distinguish mice infected with wild-type or mutant Listeria monocytogenes from noninfected control mice. Identifications of significant electrospray ionization mass spectrometry (ESI-MS) sera peak areas between Listeria-infected- and control mice were performed using t tests. ESI-MS cohort peak distributions differed from mice infected with wild-type or ∆actA Listeria versus control mice with p values of 0.00012 and 0.015, respectively. A "% wild-type Listeria peaks identified" assessment tool yielded values of 64 % for wild-type infection, 51 % for ∆actA infection, and 47 % for no infection. Receiver operator characteristic area discriminatory values were 0.97 (wild-type) and 0.82 (∆actA) versus controls. Predictive value measurements revealed overall test sensitivities of 88 % for wild-type infection and 63 % for ∆actA infection. These studies indicate that ESI-MS serum profiling holds promise for diagnosis of infection with intracellular pathogens such as Listeria and indicate that the technology could be useful in understanding the L. monocytogenes infection process.
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, 940 Stanton Young Blvd, BSMB 939, Oklahoma City, OK 73014, USA.
James R
JR
Hocker
Douglas A
DA
Drevets
Marilyn J
MJ
Dillon
Jay S
JS
Hanas
D000818:Animals; D005260:Female; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics / Q000502:physiology*; D008088:Listeriosis / Q000097:blood* / Q000235:genetics / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D044967:Serum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization