Systemic metabolism of tryptophan and its catabolites, kynurenine and 3-HAA, in mice with inflammatory arthritis.
Tryptophan is an essential amino acid. The liver is primary organ involved the oxidative catabolism of tryptophan. However, in the immune system, tryptophan and its catabolites, kynurenine and 3-hydroxyanthranilic acid (3-HAA), play an anti-inflammatory role. Rheumatoid arthritis (RA) is an autoimmune disease. Collagen induced arthritis (CIA) is an animal model of RA. Therefore, it was of interest to measure concentration of tryptophan, kynurenine and 3-HAA in mice with CIA. Concentration of tryptophan and 3-HAA was measured with HPLC methods. Concentration of kynurenine was measured with colorimetric test. mRNA expression for the kynurenine pathway genes was assessed using qRT-PCR. It has been found that in sera from diseased mice concentration of tryptophan was not changed. Concentration of kynurenine and 3-HAA was decreased. Moreover, in the livers from mice with CIA, concentration of tryptophan and kynurenine was decreased. These observations coincided with decreased mRNA expression for Ido2 and Afm and increased mRNA expression for Kynureninase in the liver. It has been also shown that in CIA the concentration of 3-HAA was increased in the kidneys.
The Kennedy Institute of Rheumatology Division, Imperial College London, Arthritis Research UK Building, Charing Cross Hospital Campus, 65 Aspenlea Road, London W6 8LH, UK. lukasz.kl@interia.pl
Lukasz
L
Kolodziej
D015095:3-Hydroxyanthranilic Acid / Q000378:metabolism*; D000818:Animals; D001169:Arthritis, Experimental / Q000235:genetics / Q000378:metabolism*; D002384:Catalysis; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000235:genetics; D007737:Kynurenine / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D014364:Tryptophan / Q000378:metabolism*
D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D015095:3-Hydroxyanthranilic Acid; D007737:Kynurenine; D014364:Tryptophan