Human skin transcriptome during superficial cutaneous wound healing.
Healing of the epidermis is a crucial process for maintaining the skin's defense integrity and its resistance to environmental threats. Compromised wound healing renders the individual readily vulnerable to infections and loss of body homeostasis. To clarify the human response of reepithelialization, we biopsied split-thickness skin graft donor site wounds immediately before and after harvesting, as well as during the healing process 3 and 7 days thereafter. In all, 25 biopsies from eight patients qualified for the study. All samples were analyzed by genome-wide microarrays. Here, we identified the genes associated with normal skin reepithelialization over time and organized them by similarities according to their induction or suppression patterns during wound healing. Our results provide the first elaborate insight into the transcriptome during normal human epidermal wound healing. The data not only reveal novel genes associated with epidermal wound healing but also provide a fundamental basis for the translational interpretation of data acquired from experimental models.
Institute of Biomedicine, Pharmacology, Biomedicum, University of Helsinki, Helsinki, Finland.
Kristo
K
Nuutila
Antti
A
Siltanen
Matti
M
Peura
Jozef
J
Bizik
Ilkka
I
Kaartinen
Hannu
H
Kuokkanen
Tapio
T
Nieminen
Ari
A
Harjula
Pertti
P
Aarnio
Jyrki
J
Vuola
Esko
E
Kankuri
D000328:Adult; D000368:Aged; D001706:Biopsy; D049109:Cell Proliferation; D004817:Epidermis / Q000503:physiopathology; D005387:Finland; D006801:Humans; D008297:Male; D008875:Middle Aged; D012313:RNA; D062326:Re-Epithelialization / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D009418:S100 Proteins / Q000235:genetics; D020858:Tissue and Organ Harvesting / Q000379:methods; D059467:Transcriptome; D014945:Wound Healing / Q000235:genetics; D014947:Wounds and Injuries / Q000235:genetics* / Q000503:physiopathology
D009418:S100 Proteins; C110294:S100A1 protein; D012313:RNA