Fast analysis of synthetic antioxidants in edible vegetable oil using trilinear component modeling of liquid chromatography-diode array detection data.
A new chromatographic methodology is presented for fast quantitative analysis of ten synthetic phenolic antioxidants in five kinds of oil samples: propyl gallate (PG), 2,4,5-trihydroxybutyrophenone (THBP), tert-butylhydroquinone (TBHQ), nordihydroguaiaretic acid (NDGA), ethoxyquin (EQ), 3-tert-butyl-4- hydroxyanisole (BHA), octyl gallate (OG), 2,6-di-tert-butyl-4-hydroxymethyphenol (Ionox-100), dodecyl gallate (DG), 3,5-di-tert-butyl-4-hydroxytoluene (BHT). The second-order calibration, with second-order advantage, based on the alternating penalty trilinear decomposition (APTLD) algorithm has shown to be an excellent tool for modeling the three-way data, where overlapping peaks, uncalibrated inteferences, and baseline drift existed, making the fast determination and resolution of the phenolic antioxidants in oils possible. Such extraction procedure in which the antioxidants of interest would be seperated is unnecessary and the ten antioxidants can be eluted within 6 mins. For the validation of the method, linearity, root-mean-square error of prediction (RMSEP) and limit of detection (LOD) have been performed. The average recovery of antioxidants ranges from 94.9 to 106.1% and the ten analytes can be adequately determined with limits of detection of 0.18-5.72 μgm l(-1).
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Jian-Yao
JY
Wang
Hai-Long
HL
Wu
Yao
Y
Chen
Yan-Mei
YM
Sun
Yong-Jie
YJ
Yu
Xiao-Hua
XH
Zhang
Ru-Qin
RQ
Yu
D000975:Antioxidants / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D008962:Models, Theoretical; D010938:Plant Oils / Q000737:chemistry*
D000975:Antioxidants; D010938:Plant Oils