Microbial metabolic engineering for L-threonine production.
L-threonine, one of the three major amino acids produced throughout the world, has a wide application in industry, as an additive or as a precursor for the biosynthesis of other chemicals. It is predominantly produced through microbial fermentation the efficiency of which largely depends on the quality of strains. Metabolic engineering based on a cogent understanding of the metabolic pathways of L-threonine biosynthesis and regulation provides an effective alternative to the traditional breeding for strain development. Continuing efforts have been made in revealing the mechanisms and regulation of L-threonine producing strains, as well as in metabolic engineering of suitable organisms whereby genetically-defined, industrially competitive L-threonine producing strains have been successfully constructed. This review focuses on the global metabolic and regulatory networks responsible for L-threonine biosynthesis, the molecular mechanisms of regulation, and the strategies employed in strain engineering.
Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, JiangnanUniversity, Wuxi, 214122, China.
Xunyan
X
Dong
Peter J
PJ
Quinn
Xiaoyuan
X
Wang
D001222:Aspartate Kinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008958:Models, Molecular; D013912:Threonine / Q000096:biosynthesis*
D013912:Threonine; D002244:Carbon; D001222:Aspartate Kinase