16S rRNA gene-targeted TTGE in determining diversity of gut microbiota during acute diarrhoea and convalescence.
The human gut microbiota play a vital role in health and nutrition but are greatly modified during severe diarrhoea due to purging and pathogenic colonization. To understand the extent of loss during and after diarrhoea, faecal samples collected from children (n=21) suffering from acute diarrhoea and from their healthy siblings (n=9) were analyzed by 16S rRNA gene-targeted universal primer polymerase chain reaction (PCR), followed by temporal temperature gradient gel electrophoresis (TTGE). The gut microbiota decreased significantly as indicated by the number of TTGE bands at day 0 of acute diarrhoea [patients vs healthy siblings: 11±0.9 vs 21.8±1.1 (mean ± standard error), p<0.01]. The number of bands showed a steady increase from day 1 to day 7; however, it remained significantly less than that in healthy siblings (15±0.9, p<0.01). These results suggest that appropriate therapeutic and post-diarrhoeal nutritional intervention might be beneficial for the early microbial restoration and recovery.
icddr,b, GPO Box 128, Dhaka 1000, Bangladesh.
Shirajum
S
Monira
Syeda Antara
SA
Shabnam
Nur Haque
NH
Alam
Hubert Ph
HP
Endtz
Alejandro
A
Cravioto
Munirul
M
Alam
D001459:Bangladesh; D003289:Convalescence; D058645:Denaturing Gradient Gel Electrophoresis; D003968:Diarrhea, Infantile / Q000208:ethnology / Q000382:microbiology* / Q000534:rehabilitation*; D004403:Dysentery / Q000208:ethnology / Q000382:microbiology / Q000534:rehabilitation; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology; D014644:Genetic Variation; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S