DNA extraction from museum specimens of parasitic Hymenoptera.
At the same time that molecular researchers are improving techniques to extract DNA from museum specimens, this increased demand for access to museum specimens has created tension between the need to preserve specimens for maintaining collections and morphological research and the desire to conduct molecular analyses. To address these concerns, we examined the suitability of non-invasive DNA extraction techniques on three species of parasitic Hymenoptera (Braconidae), and test the effects of body size (parasitoid species), age (time since collection), and DNA concentration from each extract on the probability of amplifying meaningful fragments of two commonly used genetic loci. We found that age was a significant factor for determining the probability of success for sequencing both 28S and COI fragments. While the size of the braconid parasitoids significantly affected the total amount of extracted DNA, neither size nor DNA concentration were significant factors for the amplification of either gene region. We also tested several primer combinations of various lengths, but were unable to amplify fragments longer than ~150 base pairs. These short fragments of 28S and COI were however sufficient for species identification, and for the discovery of within species genetic variation.
Department of Environmental Science Policy and Management, University of California, Berkeley, California, USA. jandersen@berkeley.edu
Jeremy C
JC
Andersen
Nicholas J
NJ
Mills
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D006927:Hymenoptera / Q000145:classification / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny
D017931:DNA Primers; D004247:DNA