Serum miR-210 as a novel biomarker for molecular diagnosis of clear cell renal cell carcinoma.
OBJECTIVE
Our objective was to evaluate the levels of miR-210 in tumor and serum samples of conventional renal cell cancer (cRCC) patients to explore whether circulating miR-210 in serum can be used as a biomarker for the detection of cRCC.


METHODS
The paired samples from primary cRCC tumors and adjacent non-tumoral renal parenchyma were collected from 32 patients with cRCC. Serum samples were obtained from 68 patients with a cRCC before surgery, 10 samples after one week of surgery, and 42 healthy individuals were included in this study. Real-time PCR was used to measure the microRNA level. The expression of miRNAs was normalized using the dCT method. Expression levels of miR-210 were compared using the Mann-Whitney U test or Wilcoxon test. Diagnostic performance of serum miR-210 level was calculated by using the receiver operating characteristic (ROC) curve.


RESULTS
The average miR-210 level was higher in primary cRCC tissues than in normal tissue (p=0.004). For serum samples, the average level of miR-210 was significantly higher in cRCC patients than in controls (p<0.001). The serum miR-210 level yielded an AUC (the areas under the ROC curve) of 0.874 with a sensitivity of 81.0% and a specificity of 79.4%. Furthermore, the average serum level of miR-210 was significantly decreased in the patients one week after the operation (p=0.001).


CONCLUSIONS
Serum mi-210 may have a potential as a novel noninvasive biomarker for the detection of cRCC.
Clinical Immunology Laboratory, North Hospital, CHU of Saint-Etienne, University of Jean-Monnet, Saint-Etienne, France.
An
A
Zhao
Guorong
G
Li
Michel
M
Péoc'h
Christian
C
Genin
Marc
M
Gigante
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult
D014408:Biomarkers, Tumor; C543800:MIRN210 microRNA, human; D035683:MicroRNAs