Development and in vitro characterization of insulin loaded whey protein and alginate microparticles.
Insulin was encapsulated into microparticles (MP) made of denaturized whey proteins (WP) and alginate (ALG) using an extrusion/cold gelation process with calcium ions. High encapsulation efficiency of 85% was obtained. Influence of insulin on polymeric viscosity and on microparticle behavior was evaluated. Insulin seemed to interact with WP chains by non covalent binding and steric hindrance. This influence was balanced by ALG addition. Nevertheless, insulin was released rapidly by diffusion at both acidic and intestinal dissolution media. Despite this fast in vitro release, WP/ALG MP showed an important enzymatic inhibition effect on trypsin and alpha-chymotrypsin. Thus, WP/ALG MP contributed to an effective insulin protection towards enzymatic degradation. The aforementioned results suggested that WP based microparticles are a promising carrier for improving oral delivery of insulin.
Université d'Auvergne, UFR Pharmacie, Equipe de Recherche Technologique Conception, Ingénierie et Développement de l'Aliment et du Médicament (ERT CIDAM), Clermont-Ferrand F-63001, France.
Emmanuelle
E
Déat-Lainé
Valérie
V
Hoffart
Jean-Michel
JM
Cardot
Muriel
M
Subirade
Eric
E
Beyssac
D000464:Alginates / Q000737:chemistry*; D002118:Calcium / Q000032:analysis; D002918:Chymotrypsin / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry*; D010316:Particle Size; D014357:Trypsin / Q000737:chemistry; D000067816:Whey Proteins
D000464:Alginates; D006603:Hexuronic Acids; D007328:Insulin; D008894:Milk Proteins; D000067816:Whey Proteins; D020723:Glucuronic Acid; D002918:Chymotrypsin; D014357:Trypsin; D002118:Calcium