High-sensitivity Orbitrap mass analysis of intact macromolecular assemblies.
The analysis of intact protein assemblies in native-like states by mass spectrometry offers a wealth of information on their biochemical and biophysical properties. Here we show that the Orbitrap mass analyzer can be used to measure protein assemblies of molecular weights approaching one megadalton with sensitivity down to the detection of single ions. Minor instrumental modifications enabled the measurement of various protein assemblies with outstanding mass-spectral resolution.
Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute of Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Rebecca J
RJ
Rose
Eugen
E
Damoc
Eduard
E
Denisov
Alexander
A
Makarov
Albert J R
AJ
Heck
D018834:Chaperonin 60 / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008970:Molecular Weight; D040901:Proteomics / Q000379:methods; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation / Q000379:methods; D053719:Tandem Mass Spectrometry
D018834:Chaperonin 60; D007074:Immunoglobulin G; D046911:Macromolecular Substances