The N-acetylcysteine-insensitive acetic acid-induced yeast programmed cell death occurs without macroautophagy.
Programmed cell death can occur through two separate pathways caused by treatment of Saccharomyces cerevisiae with acetic acid (AA-PCD), which differ from one another essentially with respect to their sensitivity to N-acetylcysteine (NAC) and to the role played by cytochrome c and metacaspase YCA1. Moreover, yeast can also undergo macroautophagy which occurs in NAC-insensitive manner. In order to gain some insight into the relationship between AA-PCD and macroautophagy use was made of WT and knock-out cells lacking YCA1 and/or cytochrome c. We show that i. macroautophagy is modulated by YCA1 and by cytochrome c in a negative and positive manner, respectively, ii. the NAC-insensitive AA-PCD and macroautophagy differ from one another and iii. NAC-insensitive AA-PCD pathway takes place essentially without macroautophagy, even if the shift of extracellular pH to acidic values required for AA-PCD to occur leads itself to increased or decreased macroautophagy in YCA1 or cytochrome c-lacking cells.
CNR - Istituto di Biomembrane e Bioenergetica, I-70126 Bari, Italy.
Lucia
L
Antonacci
Nicoletta
N
Guaragnella
Maša
M
Ždralevic
Salvatore
S
Passarella
Ersilia
E
Marra
Sergio
S
Giannattasio
D019342:Acetic Acid / Q000494:pharmacology*; D000111:Acetylcysteine / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology; D020169:Caspases / Q000235:genetics / Q000378:metabolism*; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism*; D020287:In Situ Nick-End Labeling; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D000975:Antioxidants; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D000469:Alkaline Phosphatase; D020169:Caspases; C456272:MCA1 protein, S cerevisiae; D019342:Acetic Acid; D000111:Acetylcysteine