Differential expression of phospholipase C epsilon 1 is associated with chronic atrophic gastritis and gastric cancer.
BACKGROUND
Chronic inflammation plays a causal role in gastric tumor initiation. The identification of predictive biomarkers from gastric inflammation to tumorigenesis will help us to distinguish gastric cancer from atrophic gastritis and establish the diagnosis of early-stage gastric cancer. Phospholipase C epsilon 1 (PLCε1) is reported to play a vital role in inflammation and tumorigenesis. This study was aimed to investigate the clinical significance of PLCε1 in the initiation and progression of gastric cancer.


RESULTS
Firstly, the mRNA and protein expression of PLCε1 were analyzed by reverse transcription-PCR and Western blotting in normal gastric mucous epithelial cell line GES-1 and gastric cancer cell lines AGS, SGC7901, and MGC803. The results showed both mRNA and protein levels of PLCε1 were up-regulated in gastric cancer cells compared with normal gastric mucous epithelial cells. Secondly, this result was confirmed by immunohistochemical detection in a tissue microarray including 74 paired gastric cancer and adjacent normal tissues. Thirdly, an independence immunohistochemical analysis of 799 chronic atrophic gastritis tissue specimens demonstrated that PLCε1 expression in atrophic gastritis tissues were down-regulated since PLCε1 expression was negative in 524 (65.6%) atrophic gastritis. In addition, matched clinical tissues from atrophic severe gastritis and gastric cancer patients were used to further confirm the previous results by analyzing mRNA and protein levels expression of PLCε1 in clinical samples.


CONCLUSIONS
Our results suggested that PLCε1 protein may be a potential biomarker to distinguish gastric cancer from inflammation lesion, and could have great potential in applications such as diagnosis and pre-warning of early-stage gastric cancer.
Department of Bio-Nano Science and Engineering, Key Lab of Thin Film and Microfabrication Technology of Department of Education, Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Jun
J
Chen
Wei
W
Wang
Tao
T
Zhang
Jiajia
J
Ji
Qirong
Q
Qian
Lungeng
L
Lu
Hualin
H
Fu
Weilin
W
Jin
Daxiang
D
Cui
D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D003937:Diagnosis, Differential; D055088:Early Detection of Cancer; D005757:Gastritis, Atrophic / Q000175:diagnosis / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007249:Inflammation; D054801:Phosphoinositide Phospholipase C / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics
D014408:Biomarkers, Tumor; D054801:Phosphoinositide Phospholipase C; C423320:phospholipase C epsilon