Anthelmintic and antimicrobial activity of methanolic and aqueous extracts of Euphorbia helioscopia L.
The aim of this study was to evaluate the anthelmintic and antimicrobial efficacy of Euphorbia helioscopia crude extracts. A worm motility inhibition assay and egg hatch assay were used for in vitro study, and a faecal egg count reduction assay was used for in vivo study. The in vitro study revealed anthelmintic effects of crude methanolic extracts of E. helioscopia on live Haemonchus contortus worms as evident from their paralysis and/or death at 8 h after exposure. Different concentrations (12.5 mg ml(-1), 25 mg ml(-1) and 50 mg ml(-1)) of aqueous and methanolic extracts were used against H. contortus which exhibited dose-dependent anthelmintic effects on H. contortus. Different extracts of E. helioscopia on percent inhibitory egg hatching was very low as compared to levamisole. The antimicrobial activity of extracts ranging from 100 to 500 mg ml(-1) screened by disc diffusion method against four selected bacterial (Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas multocida and Escherichia coli) and two fungal strains (Aspergillus flavus and Candida albicans) was also dose dependent with the extract showing more inhibitory effects against S. aureus and E. coli and a minimum inhibitory effect against A. flavus. It is concluded that the entire plant of E. helioscopia possesses significant anthelmintic and antimicrobial activity and could be a potential alternative for treating cases of helminth infections in ruminants.
Centre of Research for Development, University of Kashmir, Srinagar, 190006, India. bashir.lone@gmail.com
Bashir A
BA
Lone
Suhaib A
SA
Bandh
Mohammad Zahoor
MZ
Chishti
Fayaz Ahmad
FA
Bhat
Hidayatullah
H
Tak
Humeera
H
Nisa
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000969:Antinematodal Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D028481:Euphorbia / Q000737:chemistry*; D005243:Feces / Q000469:parasitology; D005260:Female; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006189:Haemonchus / Q000187:drug effects; D007194:India; D007978:Levamisole / Q000494:pharmacology; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D012756:Sheep; D012757:Sheep Diseases / Q000188:drug therapy* / Q000469:parasitology; D013211:Staphylococcus aureus / Q000187:drug effects
D000890:Anti-Infective Agents; D000969:Antinematodal Agents; D010936:Plant Extracts; D007978:Levamisole