Preserved functional and biochemical characteristics of platelet components prepared with amotosalen and ultraviolet A for pathogen inactivation.
BACKGROUND
Platelet concentrate (PC) functionality decreases during storage. This is referred to as the storage lesion. Pathogen inactivation may accelerate or induce lesions, potentially accounting for reduced viability. Our aim was to characterize functional and biochemical properties of platelets (PLTs) from photochemically treated buffy-coat PCs (PCT-PCs) compared to those from conventional PCs.


METHODS
Four PCT-PCs and four conventional PCs were stored for 6.5 days and PLT function and proteomic profiles were examined at various time points during storage. To evaluate their intrinsic properties, samples of stored PLTs were taken, washed, and suspended in Tyrode's buffer before testing.


RESULTS
PLT counts and morphology were conserved although a slight increase in the PLT volume was observed after PCT. Glycoprotein (GP) IIbIIIa, IaIIa, and VI expression remained stable while GPIbα declined similarly in both types of PCs. A steep decrease (50%) in GPV occurred on Day 1.5 in PCT-PCs and Day 2.5 in control PCs. For both PCT- and control PCs, P-selectin expression and activated GPIIbIIIa remained low during storage. PCT- and control PCs were fully responsive to aggregation agonists up to Day 4.5 and exhibited similar perfusion functionality. Mitochondrial membrane potential and annexin A5 binding of PCT-PCs and control PCs were comparable. Two-dimensional differential in-gel electrophoresis and mass spectrometry profiles for 1882 protein spots revealed only three proteins selectively changed in PCT-PCs compared to control-PCs.


CONCLUSIONS
Washed treated and untreated PCs have similar functional, morphologic, and proteomic characteristics provided that PLTs are suspended in an appropriate medium during testing.
INSERM, UMR_S949, Université de Strasbourg, Etablissement Français du Sang-Alsace (EFS-Alsace), Strasbourg, France.
Béatrice
B
Hechler
Philippe
P
Ohlmann
Philippe
P
Chafey
Catherine
C
Ravanat
Anita
A
Eckly
Eric
E
Maurer
Pierre
P
Mangin
Hervé
H
Isola
Jean-Pierre
JP
Cazenave
Christian
C
Gachet
D017304:Annexin A5 / Q000378:metabolism; D000094902:Blood Banking / Q000379:methods; D058621:Blood Buffy Coat / Q000166:cytology / Q000382:microbiology; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism / Q000382:microbiology; D001793:Blood Preservation / Q000379:methods*; D058731:Blood Safety / Q000379:methods*; D017848:Blood-Borne Pathogens / Q000528:radiation effects*; D015925:Cryopreservation / Q000379:methods; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D053078:Membrane Potential, Mitochondrial; D019007:P-Selectin / Q000378:metabolism; D017319:Photosensitizing Agents / Q000494:pharmacology; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism; D017713:Platelet Transfusion; D040901:Proteomics; D014466:Ultraviolet Rays
D017304:Annexin A5; D011564:Furocoumarins; D019007:P-Selectin; D017319:Photosensitizing Agents; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; C118577:amotosalen