Inflammatory response assessment of a hybrid tissue-engineered heart valve leaflet.
Despite substantial research in the past few decades, only slight progress has been made toward developing biocompatible, tissue-engineered scaffolds for heart valve leaflets that can withstand the dynamic pressure inside the heart. Recent progress on the development of hybrid scaffolds, which are composed of a thin metal mesh enclosed by multi-layered tissue, appear to be promising for heart valve engineering. This approach retains all the advantages of biological scaffolds while developing a strong extracellular matrix backbone to withstand dynamic loading. This study aims to test the inflammatory response of hybrid tissue-engineered leaflets based on characterizing the activation of macrophage cells cultured on the surfaces of the tissue construct. The results indicate that integration of biological layers around a metal mesh core-regardless of its type-may reduce the evoked inflammatory responses by THP-1 monocyte-like cells. This observation implies that masking a metal implant within a tissue construct prior to implantation can hide it from the immune system and may improve the implant's biocompatibility.
The Edwards Lifesciences Center for Advanced Cardiovascular Technology, Department Biomedical Engineering, University of California-Irvine, CA 92697, USA.
S Hamed
SH
Alavi
Wendy F
WF
Liu
Arash
A
Kheradvar
D000497:Alloys; D001011:Aorta / Q000166:cytology; D001672:Biocompatible Materials; D002460:Cell Line; D024042:Collagen Type I; D042783:Endothelial Cells; D005347:Fibroblasts; D006351:Heart Valves / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D032389:Myocytes, Smooth Muscle; D013193:Stainless Steel; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D014470:Umbilical Cord / Q000166:cytology
D000497:Alloys; D001672:Biocompatible Materials; D024042:Collagen Type I; D014409:Tumor Necrosis Factor-alpha; D013193:Stainless Steel; C013616:nitinol