Stereo-array isotope labeling method for studying protein structure and dynamics.
The stereo-array isotope labeling (SAIL) method utilizes proteins with isotope labeling patterns optimized with regard to an intended NMR study. The SAIL proteins are prepared by incorporating chemically synthesized amino acids into target proteins, using a cell-free protein synthesis system or a cellular expression system. Over the past decade, the SAIL method has been facilitating a wide variety of new investigations, including high-resolution structure determinations of large proteins and investigations of protein dynamics. In this chapter, the applications of SAIL-related approaches are introduced.
Graduate School of Science, Nagoya University, Nagoya, Japan.
Yohei
Y
Miyanoiri
Mitsuhiro
M
Takeda
Masatsune
M
Kainosho
D002474:Cell-Free System; D004220:Disulfides / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
D004220:Disulfides; D011506:Proteins