High prevalence of Toxoplasma gondii infection in Microtus fortis by seminested PCR from Jilin Province, Northeastern China.
Toxoplasma gondii is an important protozoan parasite that can infect virtually all warm-blooded animals, including humans. Oocysts in the environment are an important infection source. Microtus fortis can be used as a sentinel reflecting the distribution of T. gondii oocysts in the environment indirectly. In the present study, we developed a seminested polymerase chain reaction (PCR) targeting T. gondii 529-base-pair repeat sequence, with high sensitivity and specificity. Two hundred seventy-two free-living M. fortis were collected from Jilin Province, Northeastern China, and the lung samples were examined for T. gondii DNA by seminested PCR. Toxoplasma gondii DNA was found in 137 (50.4%) of these rodents. The high prevalence of T. gondii infection in free-living M. fortis suggests a wide distribution of T. gondii oocysts and a high infection pressure for humans and other animals.
Institute of Military Veterinary, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, 666 Liuying Xi Lu, Changchun 130122, Jilin Province, China.
Yingguang
Y
Zhang
Dan
D
Xu
Lili
L
Cao
Yuwei
Y
Gao
Xianzhu
X
Xia
Zhaowei
Z
Zhang
Tiecheng
T
Wang
Feng
F
Wei
Guilian
G
Yang
Quan
Q
Liu
D000818:Animals; D003411:Arvicolinae / Q000469:parasitology*; D002681:China / Q000453:epidemiology; D016054:DNA, Protozoan / Q000032:analysis; D008168:Lung / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012376:Rodent Diseases / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000175:diagnosis / Q000453:epidemiology*
D016054:DNA, Protozoan