[Effect of acrylonitrile on the expression of Bcl-2 and Bax in spermatogenic cell of mice].
OBJECTIVE
To explore the effect of acrylonitrile exposure on the expression of Bcl-2 and Bax in mice spermatogenic cells.


METHODS
Based on body weight, 250 SPF Kunming adult male mice were randomly divided into five groups: negative control group (normal saline 0.01ml/g), three AN exposure groups (intraperitoneal injection of 1.25, 2.50 or 5.00 mg/kg of AN daily for 5 days, respectively) and positive control group (intraperitoneal injection of cyclophosphamide 40mg/kg). Mice were killed in the 7th, 14th, 21st, 28th and 35th day after the first exposure by cervical dislocation. Immunohistochemical method (SABC) was used to detect the expression of Bcl-2 and Bax protein in spermatogenic cells.


RESULTS
The average optical density values of Bcl-2 at five time points of the AN 2.50 mg/kg group and the 21th day point of the AN 1.25mg/kg group were significantly lower than the negative control group (P < 0.05). Except the 21st day point of the AN 1.25 mg/kg group, the mean optical density values of Bax in all time points of AN exposure groups were significantly higher than the negative control group (P < 0.05). The decreased expression of Bcl-2 protein was most distinct in AN 2.50mg/kg group and the positive control group at all time points. The expression of Bax protein was significantly increased in all groups at the 14th day point.


CONCLUSIONS
The expression of Bcl-2 protein could be weakened in spermatogenic cells induced by AN, especially in the AN 2.50 mg/kg group; while the expression of Bax was enhanced, and the amplitude of change in the 14th day point was more obvious.
peily09@lzu.edu.cn
Lingyun
L
Pei
Guoyan
G
Ma
Na
N
Jin
Ya
Y
Chen
Yujuan
Y
Feng
Yuhui
Y
Dang
Hongli
H
Xue
Zhilan
Z
Li
D000181:Acrylonitrile / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis; D008297:Male; D051379:Mice; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000187:drug effects*
C494827:Bax protein, mouse; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D000181:Acrylonitrile