Proteomic analysis of the effect of triterpenes from Patrinia heterophylla on leukemia K562 cells.
For centuries, Patrinia heterophylla had been used in China to treat many diseases including tumor. Triterpenes has been identified as the major active constituents in Patrinia heterophylla. To elucidate the antitumor mechanism of triterpenes from Patrinia heterophylla1 (TPH), a proteomic analysis is carried out with TPH treatment in K562 cells. The total proteins extracted from TPH treated K562 cells are analyzed by two dimensional gel electrophoresis (2-DE) and compared with those untreated K562 cells. Mass spectrometry is applied to identify the differentially expressed proteins. Twenty-three differentially expressed significant proteins are discovered. Eight proteins are later identified by mass spectrometry (MALDI-TOF-MS) and Mascot software. Among them, four proteins are up-regulated (Aldolase A, Glyceraldehyde-3-phosphate dehydrogenase, Flavin reductase and Hemoglobin subunit) and four proteins were down-regulated (Heat-shock protein 90 〈Alpha〉 (HSP90-〈Alpha〉), Eukaryotic translation initiation factor 5A, Moesin, tublin) by TPH treatment in K562 cells. The identified proteins are associated with energy metabolism, oxidative stress, apoptosis, signal transduction, differential induction, and protein biosynthesis. These findings might provide valuable insights into the antitumor mechanism of TPH in K562 cells.
School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
Dong-Feng
DF
Wei
Yan-Xia
YX
Wei
Wei-Dong
WD
Cheng
Ming-Fei
MF
Yan
Gang
G
Su
Yan
Y
Hu
Ya-Qiong
YQ
Ma
Chao
C
Han
Yan
Y
Lu
Cao
C
Hui-Ming
Ying-Cun
YC
Bao
D000970:Antineoplastic Agents / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D055554:Hemoglobin Subunits / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000188:drug therapy / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D008840:Microfilament Proteins / Q000378:metabolism; D032405:Patrinia; D010448:Peptide Initiation Factors / Q000378:metabolism; D040901:Proteomics; D016601:RNA-Binding Proteins / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014315:Triterpenes / Q000494:pharmacology*; D014404:Tubulin / Q000378:metabolism; D000097574:Eukaryotic Translation Initiation Factor 5A
D000970:Antineoplastic Agents; D018841:HSP90 Heat-Shock Proteins; D055554:Hemoglobin Subunits; D008840:Microfilament Proteins; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D014315:Triterpenes; D014404:Tubulin; D000097574:Eukaryotic Translation Initiation Factor 5A; C071058:moesin