Helium-neon laser improves skin repair in rabbits.
The purpose of this study was to evaluate the influence of helium-neon laser on skin injury in rabbits. For this purpose, 15 New Zealand rabbits underwent bilateral skin damage in leg. Helium-neon laser light, at a fluence of 6 J∕cm2 and wavelength of 632.8 nm, was applied on the left legs (laser group). The right leg lesions (control group) served as negative control. All sections were histopathologically analyzed using HE sections. The results showed little infiltration of inflammatory cells, with proliferation of fibroblasts forming a few fibrous connective tissue after 1 week post-injury. The lesion on the 3rd week was characterized by granulation tissue, which formed from proliferated fibrous connective tissue, congested blood vessels and mild mononuclear cell infiltration. On the 5th week, it was observed that debris material surrounded by a thick layer of connective tissue and dense collage, fibroblasts cells present in the dermis covered by a thick epidermal layer represented by keratinized epithelium. Taken together, our results suggest that helium-neon laser is able to improve skin repair in rabbits at early phases of recovery.
Department of Human Movement Sciences, Federal University of São Paulo, UNIFESP, SP, Brazil.
Maria Stella
MS
Peccin
Ana Claudia Muniz
AC
Renno
Flavia
F
de Oliveira
Paulo Ricardo
PR
Giusti
Daniel Araki
DA
Ribeiro
D000818:Animals; D049109:Cell Proliferation; D005347:Fibroblasts; D006371:Helium; D007249:Inflammation / Q000473:pathology; D054020:Lasers, Gas / Q000627:therapeutic use*; D028022:Low-Level Light Therapy; D008297:Male; D009356:Neon; D009504:Neutrophils; D011817:Rabbits; D012867:Skin / Q000473:pathology* / Q000528:radiation effects*; D014945:Wound Healing / Q000528:radiation effects*
D006371:Helium; D009356:Neon