Clear-cut observation of PNA invasion using nanomechanical DNA origami devices.
Invasive binding event of PNA into DNA duplex was clearly observed both by atomic force microscope (AFM) imaging and electrophoretic mobility shift assay (EMSA) with the aid of nanomechanical DNA origami devices as 'single-molecule' visual probes, showing their potential as universal platform for the analysis of PNA invasion.
RCAST, The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8904, Japan.
Takahiro
T
Yamazaki
Yuichiro
Y
Aiba
Kohei
K
Yasuda
Yusuke
Y
Sakai
Yusei
Y
Yamanaka
Akinori
A
Kuzuya
Yuichi
Y
Ohya
Makoto
M
Komiyama
D004247:DNA / Q000737:chemistry*; D024202:Electrophoretic Mobility Shift Assay; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000737:chemistry*
D020135:Peptide Nucleic Acids; D004247:DNA