Bone tissue engineering of induced pluripotent stem cells cultured with macrochanneled polymer scaffold.
A reliable source of osteogenic cells is an essential factor for bone tissue engineering. In this study, human-induced pluripotent stem cells (hiPSCs) without an embryoid body step were cultured in macrochanneled poly(caprolactone) (PCL) scaffolds prepared using a robotic dispensing technique, after which osteogenesis was promoted by the addition of exogenous osteogenic factors. The osteogenesis of the hiPSCs was demonstrated based on the detection of osteogenic molecules, such as osteopontin, using flow cytometry analysis, quantitative polymerase chain reaction and western blotting. Thereafter, the cell-scaffold constructs were transplanted into the subcutaneous site of male athymic mice. At 4 weeks after implantation, histological assays (hematoxylin & eosin staining, Alizarin red staining, and osteocalcin immunostaining) were conducted to determine the bone induction of hiPSCs. The results indicated a production of pronounced levels of extracellular matrices and their mineral deposition within the cell-scaffold implant, suggesting possible in vivo bone induction by the hiPSCs-based tissue engineering approach. The results presented here provide useful information regarding the tissue engineering of bone utilizing hiPSCs in conjunction with cell-supporting scaffolds.
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, South Korea.
Guang-Zhen
GZ
Jin
Tae-Hyun
TH
Kim
Joong-Hyun
JH
Kim
Jong-Eun
JE
Won
So-Young
SY
Yoo
Seong-Jun
SJ
Choi
Jung Keun
JK
Hyun
Hae-Won
HW
Kim
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000637:transplantation*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010006:Osteoblasts / Q000166:cytology; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone