A new vesicular scaffolding complex mediates the G-protein-coupled 5-HT1A receptor targeting to neuronal dendrites.
Although essential for their neuronal function, the molecular mechanisms underlying the dendritic targeting of serotonin G-protein-coupled receptors are poorly understood. Here, we characterized a Yif1B-dependent vesicular scaffolding complex mediating the intracellular traffic of the rat 5-HT(1A) receptor (5-HT(1A)R) toward dendrites. By combining directed mutagenesis, GST-pull down, and surface plasmon resonance, we identified a tribasic motif in the C-tail of the 5-HT(1A)R on which Yif1B binds directly with high affinity (K(D) ≈ 37 nM). Moreover, we identified Yip1A, Rab6, and Kif5B as new partners of the 5-HT(1A)R/Yif1B complex, and showed that their expression in neurons is also crucial for the dendritic targeting of the 5-HT(1A)R. Live videomicroscopy revealed that 5-HT(1A)R, Yif1B, Yip1A, and Rab6 traffic in vesicles exiting the soma toward the dendritic tree, and also exhibit bidirectional motions, sustaining their role in 5-HT(1A)R dendritic targeting. Hence, we propose a new trafficking pathway model in which Yif1B is the scaffold protein recruiting the 5-HT(1A)R in a complex including Yip1A and Rab6, with Kif5B and dynein as two opposite molecular motors coordinating the traffic of vesicles along dendritic microtubules. This targeting pathway opens new insights for G-protein-coupled receptors trafficking in neurons.
INSERM U894, Centre de Psychiatrie et Neurosciences, Paris F-75014, France.
Sana
S
Al Awabdh
Stéphanie
S
Miserey-Lenkei
Tahar
T
Bouceba
Justine
J
Masson
Fumi
F
Kano
Carine
C
Marinach-Patrice
Michel
M
Hamon
Michel Boris
MB
Emerit
Michèle
M
Darmon
D000818:Animals; D002478:Cells, Cultured; D003712:Dendrites / Q000235:genetics / Q000502:physiology*; D018390:Gene Targeting / Q000379:methods; D006801:Humans; D045170:Matrix Attachment Regions / Q000235:genetics / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D021381:Protein Transport / Q000235:genetics / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D044282:Receptor, Serotonin, 5-HT1A / Q000235:genetics / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D013572:Synaptic Vesicles / Q000235:genetics / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000502:physiology*
D009419:Nerve Tissue Proteins; D043562:Receptors, G-Protein-Coupled; D033921:Vesicular Transport Proteins; C531168:Yif1B protein, rat; D044282:Receptor, Serotonin, 5-HT1A