The Junctional Adhesion Molecule-B regulates JAM-C-dependent melanoma cell metastasis.
Metastasis is a major clinical issue and results in poor prognosis for most cancers. The Junctional Adhesion Molecule-C (JAM-C) expressed by B16 melanoma and endothelial cells has been involved in metastasis of tumor cells through homophilic JAM-C/JAM-C trans-interactions. Here, we show that JAM-B expressed by endothelial cells contributes to murine B16 melanoma cells metastasis through its interaction with JAM-C on tumor cells. We further show that this adhesion molecular pair mediates melanoma cell adhesion to primary Lung Microvascular Endothelial Cells and that it is functional in vivo as demonstrated by the reduced metastasis of B16 cells in Jam-b deficient mice.
Centre de Recherche en Cancérologie de Marseille, Unité Mixte de Recherche, Institut National de la Santé et de la Recherche Médicale 1068/Centre National de la Recherche Scientifique 7258, Marseille, France. marie-laure.arcangeli@inserm.fr
Marie-Laure
ML
Arcangeli
Vincent
V
Frontera
Florence
F
Bardin
Jeanne
J
Thomassin
Bruno
B
Chetaille
Susanne
S
Adams
Ralf H
RH
Adams
Michel
M
Aurrand-Lions
D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D008168:Lung / Q000098:blood supply / Q000378:metabolism; D008297:Male; D008546:Melanoma, Experimental / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018613:Microscopy, Confocal; D009362:Neoplasm Metastasis; D011485:Protein Binding; D034622:RNA Interference; D062765:Junctional Adhesion Molecule B
D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C498781:Jam2 protein, mouse; D062765:Junctional Adhesion Molecule B; C486723:Jam3 protein, mouse