Enhanced carboxymethylcellulase production by a newly isolated marine bacterium, Cellulophaga lytica LBH-14, using rice bran.
The aim of this work was to establish the optimal conditions for production of carboxymethylcellulase (CMCase) by a newly isolated marine bacterium using response surface methodology (RSM). A microorganism producing CMCase, isolated from seawater, was identified as Cellulophaga lytica based 16S rDNA sequencing and the neighborjoining method. The optimal conditions of rice bran, ammonium chloride, and initial pH of the medium for cell growth were 100.0 g/l, 5.00 g/l, and 7.0, respectively, whereas those for production of CMCase were 79.9 g/l, 8.52 g/l, and 6.1. The optimal concentrations of K2HPO4, NaCl, MgSO4·7H2O, and (NH4)2SO4 for cell growth were 6.25, 0.62, 0.28, and 0.42 g/l, respectively, whereas those for production of CMCase were 3.72, 0.54, 0.70, and 0.34 g/l. The optimal temperature for cell growth and the CMCase production by C. lytica LBH-14 were 35 degrees C and 25 degrees C, respectively. The maximal production of CMCase under optimized condition for 3 days was 110.8 U/ml, which was 5.3 times higher than that before optimization. In this study, rice bran and ammonium chloride were developed as carbon and nitrogen sources for the production of CMCase by C. lytica LBH-14. The time for production of CMCase by a newly isolated marine bacterium with submerged fermentations reduced to 3 days, which resulted in enhanced productivity of CMCase and a decrease in its production cost.
Department of Medical Bioscience, Graduate School of Donga-A University, Busan 604-714, Korea.
Wa
W
Gao
Eun-Jung
EJ
Lee
Sang-Un
SU
Lee
Jianhong
J
Li
Chung-Han
CH
Chung
Jin-Woo
JW
Lee
D000643:Ammonium Chloride / Q000737:chemistry; D001426:Bacterial Proteins / Q000096:biosynthesis; D001431:Bacteriological Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002480:Cellulase / Q000096:biosynthesis*; D003470:Culture Media / Q000737:chemistry / Q000592:standards; D005285:Fermentation; D041965:Flavobacteriaceae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D005798:Genes, Bacterial; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D008278:Magnesium Sulfate / Q000737:chemistry; D009584:Nitrogen / Q000737:chemistry; D012275:Oryza / Q000737:chemistry*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012044:Regression Analysis; D012965:Sodium Chloride / Q000737:chemistry; D013997:Time Factors; D014871:Water Microbiology
D001426:Bacterial Proteins; D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D000643:Ammonium Chloride; D012965:Sodium Chloride; D002244:Carbon; D008278:Magnesium Sulfate; D002480:Cellulase; C019826:carboxymethylcellulase; D009584:Nitrogen