Light-induced cell detachment for cell sheet technology.
The phenomenon of light-induced cell detachment is reported. Mouse calvaria-derived, pre-osteoblastic (MC3T3-E1) cells were cultured on a TiO(2) nanodot-coated quartz substrate. After 20 min of UV365 illumination, over 90% of the cells would detach from the surface. Moreover, intact cell sheets could be obtained in the same way. It was found that the as-obtained cells showed good viability, and could be used for further culture processes and other applications. Also, biocompatibility and safety characterizations indicated that the use of TiO(2) nanodots and UV365 illumination was safe for such cell detachment. It is suggested that adsorbed extracellular matrix proteins play key roles in developing cell sheets and ensuring biocompatibility. The present light-induced cell detachment method demonstrates a promising way for rapid cell/cell sheet harvesting.
Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
Yi
Y
Hong
Mengfei
M
Yu
Wenjian
W
Weng
Kui
K
Cheng
Huiming
H
Wang
Jun
J
Lin
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000528:radiation effects; D048430:Cell Shape / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004578:Electron Spin Resonance Spectroscopy; D005434:Flow Cytometry; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000528:radiation effects*; D056951:Photoelectron Spectroscopy; D011506:Proteins / Q000737:chemistry; D013997:Time Factors; D014025:Titanium / Q000737:chemistry; D014466:Ultraviolet Rays; D014867:Water / Q000737:chemistry
D011506:Proteins; D014867:Water; C009495:titanium dioxide; D014025:Titanium