Molecular characterization of the measles virus genotypes in JiLin Province, China.
Measles remains a severe global health threat, and nearly 30 million new cases are reported annually. Although many studies have analyzed measles viruses (MV) at the epidemiologic and phylogenetic levels, no study has yet to integrate these two types of data. To this end, we isolated 16 wild-type MV strains China's Jilin province. The MV genotype H1 was the most prevalent strain. After sequencing the nucleoprotein (N) genes of these strains, a maximum clade credibility tree was constructed by the bayesian Markov Chain Monte Carlo method using 450 MV strains from GenBank with epidemiological information. The MV N gene evolution rate was 1.127E-3. Analysis of the time of the most recent common ancestor (TMRCA) for genotypes A/B/C/G/H revealed that genotypes D and B had the largest and smallest TMRCA (45.86 and 26.63, respectively). The highest level of genetic diversity for the MV N gene occurred around the year 2000. Here in this study, we uncovered the MV genotypes circulating in China's Jilin Province and estimated the epidemiologic and phylogenetic relationship for the six different genotypes of MV.
Department of Pathogeny Biology, Norman Bethune Medical College of Jilin University, Changchun, Jilin, China.
Chengguo
C
Wei
Jingwei
J
Shi
Bin
B
Liu
Yue
Y
Shi
Jingtong
J
Zheng
Guangyu
G
Xu
Jinshu
J
Ma
Guoqing
G
Wang
Fan
F
Li
D000293:Adolescent; D001483:Base Sequence; D001499:Bayes Theorem; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D019143:Evolution, Molecular; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007223:Infant; D008390:Markov Chains; D008457:Measles / Q000453:epidemiology / Q000821:virology*; D008459:Measles virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009010:Monte Carlo Method; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics*; D055815:Young Adult
D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D014764:Viral Proteins; C058314:nucleoprotein, Measles virus