Dual catalysis mode for the dicarbonyl reduction catalyzed by diketoreductase.
Diketoreductase catalyzes a two-step bioreduction on a dicarbonyl substrate through a novel dual catalysis mode, in which random hydride attack simultaneously forms two mono-carbonyl intermediates, and subsequently distinct catalytic sites are responsible for the reductions of respective carbonyl group of the intermediates to yield the final dihydroxy product.
Laboratory of Chemical Biology, China Pharmaceutical University, 24 Tongjia Street, Nanjing, Jiangsu Province 210009, People's Republic of China.
Meiling
M
Lu
Yan
Y
Huang
Mark A
MA
White
Xuri
X
Wu
Nan
N
Liu
Xiaodong
X
Cheng
Yijun
Y
Chen
D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D020134:Catalytic Domain; D006860:Hydrogen Bonding; D007659:Ketones / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D013379:Substrate Specificity
D007659:Ketones; D000429:Alcohol Oxidoreductases