The crystal structures of the α-subunit of the α(2)β          (2) tetrameric Glycyl-tRNA synthetase.
Aminoacyl-tRNA synthetases (AARSs) are ligases (EC.6.1.1.-) that catalyze the acylation of amino acids to their cognate tRNAs in the process of translating genetic information from mRNA to protein. Their amino acid and tRNA specificity are crucial for correctly translating the genetic code. Glycine is the smallest amino acid and the glycyl-tRNA synthetase (GlyRS) belongs to Class II AARSs. The enzyme is unusual because it can assume different quaternary structures. In eukaryotes, archaebacteria and some bacteria, it forms an α(2) homodimer. In some bacteria, GlyRS is an α(2)β(2) heterotetramer and shows a distant similarity to α(2) GlyRSs. The human pathogen eubacterium Campylobacter jejuni GlyRS (CjGlyRS) is an α(2)β(2) heterotetramer and is similar to Escherichia coli GlyRS; both are members of Class IIc AARSs. The two-step aminoacylation reaction of tetrameric GlyRSs requires the involvement of both α- and β-subunits. At present, the structure of the GlyRS α(2)β(2) class and the details of the enzymatic mechanism of this enzyme remain unknown. Here we report the crystal structures of the catalytic α-subunit of CjGlyRS and its complexes with ATP, and ATP and glycine. These structures provide detailed information on substrate binding and show evidence for a proposed mechanism for amino acid activation and the formation of the glycyl-adenylate intermediate for Class II AARSs.
Center for Structural Genomics of Infectious Diseases, University of Chicago, Chicago, IL 60637, USA.
Kemin
K
Tan
Min
M
Zhou
Rongguang
R
Zhang
Wayne F
WF
Anderson
Andrzej
A
Joachimiak
D000255:Adenosine Triphosphate / Q000737:chemistry; D000595:Amino Acid Sequence; D016123:Campylobacter jejuni / Q000201:enzymology* / Q000235:genetics; D020134:Catalytic Domain; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray / Q000379:methods; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D005998:Glycine / Q000737:chemistry; D006032:Glycine-tRNA Ligase / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D025941:Protein Interaction Mapping; D017433:Protein Structure, Secondary; D012343:RNA, Transfer / Q000737:chemistry*; D046249:Transfer RNA Aminoacylation; D014961:X-Ray Diffraction
D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D006032:Glycine-tRNA Ligase; D005998:Glycine