Label-free imaging of cerebral β-amyloidosis with extended-focus optical coherence microscopy.
We demonstrate label-free imaging of cerebral β-amyloidosis ex vivo and in a living mouse model of Alzheimer's disease using extended-focus Fourier domain optical coherence microscopy (xfOCM). xfOCM provides 3D, high-resolution images of individual β-amyloid plaques in the brain parenchyma and vasculature and requires no staining of the alzheimeric sample under investigation. xfOCM also opens the possibility to perform minimally invasive studies of β-amyloid pathology in vivo, without the use of labeling methods, which potentially confound experimental findings.
Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.
Tristan
T
Bolmont
Arno
A
Bouwens
Christophe
C
Pache
Mitko
M
Dimitrov
Corinne
C
Berclaz
Martin
M
Villiger
Bettina M
BM
Wegenast-Braun
Theo
T
Lasser
Patrick C
PC
Fraering
D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D016657:Cerebral Amyloid Angiopathy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D005583:Fourier Analysis; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D059906:Neuroimaging / Q000295:instrumentation / Q000379:methods; D058225:Plaque, Amyloid / Q000235:genetics / Q000473:pathology; D013194:Staining and Labeling; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods*
D016229:Amyloid beta-Peptides