Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses.
Polyvinyl alcohol nanofibers incorporating the wide spectrum antibiotic gentamicin were prepared by Nanospider™ needleless technology. A polyvinyl alcohol layer, serving as a drug reservoir, was covered from both sides by polyurethane layers of various thicknesses. The multilayered structure of the nanofibers was observed using scanning electron microscopy, the porosity was characterized by mercury porosimetry, and nitrogen adsorption/desorption measurements were used to determine specific surface areas. The stability of the gentamicin released from the electrospun layers was proved by high-performance liquid chromatography (HPLC) and inhibition of bacterial growth. Drug release was investigated using in vitro experiments with HPLC/MS quantification, while the antimicrobial efficacy was evaluated on Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Both experiments proved that the released gentamicin retained its activity and showed that the retention of the drug in the nanofibers was prolonged with the increasing thickness of the covering layers.
Department of Polymer Gels, Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Prague, Czech Republic. sirc@imc.cas.cz
Jakub
J
Sirc
Sarka
S
Kubinova
Radka
R
Hobzova
Denisa
D
Stranska
Petr
P
Kozlik
Zuzana
Z
Bosakova
Dana
D
Marekova
Vladimir
V
Holan
Eva
E
Sykova
Jiri
J
Michalek
D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000737:chemistry; D004058:Diffusion; D004563:Electrochemistry / Q000379:methods; D005839:Gentamicins / Q000008:administration & dosage*; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000502:physiology*; D008422:Materials Testing; D053769:Nanocapsules / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D012399:Rotation
D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D005839:Gentamicins; D053769:Nanocapsules