Imaging neural activity using Thy1-GCaMP transgenic mice.
The ability to chronically monitor neuronal activity in the living brain is essential for understanding the organization and function of the nervous system. The genetically encoded green fluorescent protein-based calcium sensor GCaMP provides a powerful tool for detecting calcium transients in neuronal somata, processes, and synapses that are triggered by neuronal activities. Here we report the generation and characterization of transgenic mice that express improved GCaMPs in various neuronal subpopulations under the control of the Thy1 promoter. In vitro and in vivo studies show that calcium transients induced by spontaneous and stimulus-evoked neuronal activities can be readily detected at the level of individual cells and synapses in acute brain slices, as well as chronically in awake, behaving animals. These GCaMP transgenic mice allow investigation of activity patterns in defined neuronal populations in the living brain and will greatly facilitate dissecting complex structural and functional relationships of neural networks.
McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Qian
Q
Chen
Joseph
J
Cichon
Wenting
W
Wang
Li
L
Qiu
Seok-Jin R
SJ
Lee
Nolan R
NR
Campbell
Nicholas
N
Destefino
Michael J
MJ
Goard
Zhanyan
Z
Fu
Ryohei
R
Yasuda
Loren L
LL
Looger
Benjamin R
BR
Arenkiel
Wen-Biao
WB
Gan
Guoping
G
Feng
D000367:Age Factors; D000818:Animals; D001703:Biophysics; D001921:Brain / Q000166:cytology*; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism; D002452:Cell Count; D002461:Cell Line, Transformed; D003712:Dendrites / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018613:Microscopy, Confocal; D009154:Mutation / Q000235:genetics; D009219:Myosin-Light-Chain Kinase / Q000235:genetics / Q000378:metabolism; D009474:Neurons / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D009812:Odorants; D018408:Patch-Clamp Techniques; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D012160:Retina / Q000166:cytology*; D018800:Thy-1 Antigens / Q000235:genetics; D014162:Transfection
D002147:Calmodulin; C107660:M13 protein (myosin light-chain kinase); D010446:Peptide Fragments; D018800:Thy-1 Antigens; D049452:Green Fluorescent Proteins; D011189:Potassium Chloride; D009219:Myosin-Light-Chain Kinase; D002118:Calcium