Mimotope identification using phage displayed random peptide libraries against monoclonal antibodies specific to house dust mite.
Random heptapeptide T7 and random 12mer M13 phage libraries were employed to identify mimotopes binding to monoclonal antibodies (MAb) specific to house dust mite. After selection of bound phage by bio-panning and determination of binding specificity, DNA of selected bound phages was amplified, sequenced and aligned for peptide similarity. Eight mimotopes which were partially matched with Der f 15 allergen were predominant. The amino acid regions, 411-429 and 480-503 of Der f 15 allergen, appeared to be the main epitope clusters. Five mimotopes of MAb B2 and one mimotope of MAb B1 matched with Der p 1 and Der f 2 precursor, respectively.
Center of Excellence for Antibody Research (CEAR), Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Nipaporn
N
Tewawong
Pannamthip
P
Pitaksajjkul
Paron
P
Dekumyoy
Pattama
P
Ekpo
Pongrama
P
Ramasoota
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D017344:Genes, Insect; D006801:Humans; D019151:Peptide Library; D016133:Polymerase Chain Reaction; D039981:Pyroglyphidae / Q000145:classification / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA
D000911:Antibodies, Monoclonal; D019151:Peptide Library