2C-Methyl- D- erythritol 2,4-cyclodiphosphate synthase from Stevia rebaudiana Bertoni is a functional gene.
Stevia [Stevia rebaudiana (Bertoni)] is a perennial herb which accumulates sweet diterpenoid steviol glycosides (SGs) in its leaf tissue. SGs are synthesized by 2C-methyl-D-erythritol 4-phosphate (MEP) pathway. Of the various enzymes of the MEP pathway, 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (MDS) (encoded by MDS) catalyzes the cyclization of 4-(cytidine 5' diphospho)-2C-methyl-D-erythritol 2-phosphate into 2C-methyl-D-erythritol 2,4-cyclodiphosphate. Complementation of the MDS knockout mutant strain of Escherichia coli, EB370 with putative MDS of stevia (SrMDS) rescued the lethal mutant, suggesting SrMDS to be a functional gene. Experiments conducted in plant growth chamber and in the field suggested SrMDS to be a light regulated gene. Indole 3-acetic acid (IAA; 50, 100 μM) down-regulated the expression of SrMDS at 4 h of the treatment, whereas, abscisic acid did not modulate its expression. A high expression of SrMDS was observed during the light hours of the day as compared to the dark hours. The present work established functionality of SrMDS and showed the role of light and IAA in regulating expression of SrMDS.
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology (Council of Scientific and Industrial Research), Palampur, 176061, Himachal Pradesh, India. hitesh_biotech@hotmail.com
Hitesh
H
Kumar
Kashmir
K
Singh
Sanjay
S
Kumar
D000040:Abscisic Acid / Q000494:pharmacology; D004896:Erythritol / Q000031:analogs & derivatives* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D017343:Genes, Plant / Q000235:genetics*; D005816:Genetic Complementation Test; D007210:Indoleacetic Acids / Q000494:pharmacology; D008027:Light; D009154:Mutation / Q000235:genetics; D019761:Phosphorus-Oxygen Lyases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017433:Protein Structure, Secondary; D036625:Stevia / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; C443879:methyl-D-erythritol 2,4-cyclodiphosphate; C030737:indoleacetic acid; D000040:Abscisic Acid; D019761:Phosphorus-Oxygen Lyases; D004896:Erythritol