Molecular variability of sweet potato chlorotic stunt virus (SPCSV) and five potyviruses infecting sweet potato in China.
To obtain a better understanding of the molecular variation of sweet potato viruses in China, 131 samples were collected from symptomatic sweet potato plants and used for RT-PCR analysis of the heat shock protein 70 (hsp70) gene sequence of sweet potato chlorotic stunt virus (SPCSV) and the coat protein (CP) gene sequences of five potyviruses (SPFMV, SPVC, SPVG, SPLV and SPV2). The hsp70 sequences that were obtained provided evidence for the presence of two distinct strains of SPCSV. Analysis of the CP sequences amplified from the samples indicated that all five potyviruses infect sweet potato in China, and three different strains of SPFMV and two of SPVG were found.
Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Yanhong
Y
Qin
Zhenchen
Z
Zhang
Qi
Q
Qiao
Desheng
D
Zhang
Yuting
Y
Tian
Yongjiang
Y
Wang
D002681:China; D016000:Cluster Analysis; D029383:Crinivirus / Q000235:genetics* / Q000302:isolation & purification; D014644:Genetic Variation; D027723:Ipomoea batatas / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D012367:RNA, Viral; D014764:Viral Proteins