Simultaneous detection of duplex DNA oligonucleotides using a SERS-based micro-network gradient chip.
We report the development of a programmable surface-enhanced Raman scattering (SERS)-based micro-network gradient platform to simultaneously detect two different types of DNA oligomer mixtures. The utility of this platform was demonstrated by quantitative analysis of two breast cancer-related (BRCA1) DNA oligomer mixtures. To generate on-demand concentration gradients, the microfluidic circuit was designed using an electric-hydraulic analogy. Then a multi-gradient microfluidic channel was fabricated based on the theoretical design of the concentration control module. These micro-network structures automatically produce a series of different concentration gradients by continuously mixing Cy3-labeled DNA oligomers (BRAC1-Mutation) with TAMRA-labeled DNA oligomer (BRAC1-Wild). The SERS signals for different ratios of duplex DNA oligomer mixtures, adsorbed on the surface of silver nanoparticles, were measured under flowing conditions. Total analysis time from serial mixing to SERS detection takes less than 10 min because all experimental conditions are automatically controlled inside the exquisitely designed microfluidic channel. This novel SERS-based DNA sensing technology in a micro-network gradient channel is expected to be a powerful analytical tool to simultaneously detect multiple DNA oligomer mixtures.
Department of Bionano Engineering, Hanayng University, Ansan 426-791, South Korea.
Namhyun
N
Choi
Kangsun
K
Lee
Dong Woo
DW
Lim
Eun Kyu
EK
Lee
Soo-Ik
SI
Chang
Kwang W
KW
Oh
Jaebum
J
Choo
D019313:BRCA1 Protein / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D013499:Surface Properties; D013997:Time Factors
D019313:BRCA1 Protein; D009838:Oligodeoxyribonucleotides; D004247:DNA