The relative brightness of PEG lipid-conjugated polymer nanoparticles as fluid-phase markers in live cells.
While conjugated polymer nanoparticles (CPNs) have been widely touted as ultra-bright labels for biological imaging, no direct comparative measurements of their intracellular brightness have been reported. Simple in vitro comparisons are not definitive since fluorophore brightness in vitro may not correspond with intracellular brightness. We have compared the fluorescence brightness of J774A.1 cells loaded with 24 nm methoxy-capped 2,000 M(r) polyethylene glycol lipid PFBT nanoparticles (PEG lipid-PFBT CPNs) to cells loaded with carboxy-functionalized quantum dots (Qdots) or a dextran-linked small molecule organic dye, Alexa Fluor 488 dextran (AF488-dex). Under conditions likely to be used for biological imaging or flow cytometry, these CPNs are 175× brighter than Qdots and 1,400× brighter than AF488-dex in cells. Evaluation of the minimum incubation concentration required for detection of nanoparticle fluorescence with a commercial flow cytometer indicated that the limit of detection for PEG lipid-PFBT CPNs was 19 pM (86 ppb), substantially lower than values obtained for Qdots (980 pM) or AF488-dex (11.2 nM). Investigation of the mechanism of cellular uptake of the three fluid-phase labels indicates that these particles are passively taken into macrophage cells via macropinocytosis without interaction with cell surface receptors, and ultimately localize in lysosomes. In addition, no cytotoxicity could be observed at any of the CPN concentrations tested. Together, these data suggest that these CPNs are appropriate and attractive candidates as fluid-phase markers with significantly greater fluorescence brightness than existing dyes or nanoparticles. We expect that these CPNs will find application in both imaging and flow cytometry.
Department of Chemistry, Clemson University, Clemson, SC 29634, USA.
Lawrence P
LP
Fernando
Prakash K
PK
Kandel
P Christine
PC
Ackroyd
Kenneth A
KA
Christensen
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003911:Dextrans / Q000032:analysis / Q000737:chemistry; D005449:Fluorenes / Q000032:analysis / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000032:analysis / Q000737:chemistry*; D010873:Pinocytosis; D011092:Polyethylene Glycols / Q000032:analysis / Q000737:chemistry*; D011108:Polymers / Q000032:analysis / Q000737:chemistry*; D045663:Quantum Dots
D003911:Dextrans; D005449:Fluorenes; D005456:Fluorescent Dyes; D011108:Polymers; C556103:poly((9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-(2,1',3)-thiadiazole)); D011092:Polyethylene Glycols