Gene expression changes in peripheral blood from Chinese Han patients with Tourette syndrome.
To evaluate whether gene expression in chromosome 15q13-q22.3 region is responsible for the development of Tourette syndrome (TS). Eighty-four unrelated Chinese Han patients with TS (male/female = 68/16; mean age 9.92 ± 3.98 years) and 100 sex, age, and ethnicity matched normal controls (male/female = 80/20; mean age 10.90 ± 5.86 years) were enrolled in this study. We performed quantitative real-time PCR on a subset of seven genes: the L-histidine decarboxylase gene (HDC), the HECT domain and RCC-1 like domain 1 gene (HERC1), the HECT domain and RCC-1 like domain 2 gene (HERC2), the cholinergic receptor, neuronal nicotinic alpha polypeptide 7 gene (CHRNA7), the ubiquitin protein ligase E3A gene (UBE3A), the ubiquitin specific peptidase 3 gene (USP3) and the amyloid precursor protein-binding protein A2 gene (APBA2) previously reported to be stably expressed in brain tissue. A significant difference was shown for the APBA2 gene expression of peripheral lymphocytes between Chinese Han TS group and healthy controls (relative expression: 0.21 ± 0.16-fold decrease in patients versus normal, P < 0.01). Indicating that the APBA2 gene is a promising peripheral blood biomarker that discriminates between patients with TS and healthy subjects. Further studies into this gene and its protein products may provide insights into the pathogenesis of TS.
Center for Experimental Medicine, the Third Xiangya Hospital, Central South University, Changsha, Hunan Province, China.
Jing
J
Lei
Hongbo
H
Xu
Hui
H
Liang
Linyan
L
Su
Jie
J
Zhang
Xian
X
Huang
Zhi
Z
Song
Weidong
W
Le
Hao
H
Deng
D000293:Adolescent; D044466:Asian People / Q000235:genetics; D015820:Cadherins / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D002681:China; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics*; D005879:Tourette Syndrome / Q000097:blood / Q000175:diagnosis / Q000208:ethnology / Q000235:genetics*
C491636:APBA2 protein, human; D015820:Cadherins; D002352:Carrier Proteins; D005819:Genetic Markers; D009419:Nerve Tissue Proteins