Design of primer pairs for species-specific diagnosis of Leishmania (Leishmania) infantum chagasi using PCR.
The objective of this study was to design and evaluate new primers for species-specific detection of L. infantum chagasi using PCR. Two combinations of primer pairs were established with the aim of obtaining specific amplification products from the L. infantum chagasi 18S rRNA gene. The combinations of the primer pairs and the respective sizes of the PCR products, based on the U422465 GenBank reference sequence of L. infantum chagasi, were: LCS1/LCS3 (259 bp) and LCS2/LCS3 (820 bp). It was concluded that the new PCR assays optimized using the primer pairs LCS1/LCS3 and LCS2/LCS3 were effective for specific detection of L. infantum chagasi, with analytical sensitivity to detect 1 pg/µL of DNA.
Laboratório de Doenças Parasitárias, Escola de Veterinária, Universidade Federal de Goiás, Goiânia, GO, Brasil. oswaldo_neto@hotmail.com
Osvaldo José
OJ
da Silveira Neto
Sabrina Castilho
SC
Duarte
Hérika Xavier
HX
da Costa
Guido Fontgalland Coelho
GF
Linhares
D017931:DNA Primers; D018314:Leishmania infantum / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D017931:DNA Primers