A GAL4-driver line resource for Drosophila neurobiology.
We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.
Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Arnim
A
Jenett
Gerald M
GM
Rubin
Teri-T B
TT
Ngo
David
D
Shepherd
Christine
C
Murphy
Heather
H
Dionne
Barret D
BD
Pfeiffer
Amanda
A
Cavallaro
Donald
D
Hall
Jennifer
J
Jeter
Nirmala
N
Iyer
Dona
D
Fetter
Joanna H
JH
Hausenfluck
Hanchuan
H
Peng
Eric T
ET
Trautman
Robert R
RR
Svirskas
Eugene W
EW
Myers
Zbigniew R
ZR
Iwinski
Yoshinori
Y
Aso
Gina M
GM
DePasquale
Adrianne
A
Enos
Phuson
P
Hulamm
Shing Chun Benny
SC
Lam
Hsing-Hsi
HH
Li
Todd R
TR
Laverty
Fuhui
F
Long
Lei
L
Qu
Sean D
SD
Murphy
Konrad
K
Rokicki
Todd
T
Safford
Kshiti
K
Shaw
Julie H
JH
Simpson
Allison
A
Sowell
Susana
S
Tae
Yang
Y
Yu
Christopher T
CT
Zugates
D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000378:metabolism; D016208:Databases, Factual; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D009420:Nervous System / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D014157:Transcription Factors