Expressed sequence tag-PCR markers for identification of alien barley  chromosome 2H in wheat.
We developed EST-PCR markers specific to barley chromosome  2H, for the purpose of effectively tracing alien chromosomes or chromosome  parts in the wheat genetic background. The target alien chromosome 2H confers  high resistance to pre-harvest sprouting, which is a worldwide natural disaster  in wheat. A total of 120 primer pairs were selected by combining the wheat  group 2 chromosomes of the EST database and the genome sequences of the new  model plant Brachypodium distachyon. Seventy-seven of 120 primer pairs  were polymorphic and 31 of 120 primer pairs were monomorphic between a set of  wheat-barley chromosome 2H disomic addition/substitution lines and their  parents by agarose gel electrophoresis and polyacrylamide gel electrophoresis.  Thirty of 77 polymorphic primer pairs including primer pair P120 derived from  the basi gene were chromosome 2H-specific. These markers are expected to  be valuable in screening of wheat-barley chromosome 2H recombination lines and  pre-harvest sprouting resistant varieties.
College of Plant Science, Jilin University, Changchun, China.
M J
MJ
Wang
H D
HD
Zou
Z S
ZS
Lin
Y
Y
Wu
X
X
Chen
Y P
YP
Yuan
D001483:Base Sequence; D032461:Chromosomes, Plant; D017931:DNA Primers / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D020224:Expressed Sequence Tags / Q000378:metabolism*; D017343:Genes, Plant / Q000235:genetics; D005819:Genetic Markers; D001467:Hordeum / Q000235:genetics*; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D016133:Polymerase Chain Reaction / Q000379:methods*; D016415:Sequence Alignment; D014908:Triticum / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers