Potential of the microbial community present in an unimpacted beach sediment to remediate petroleum hydrocarbons.
The potential of the microbial communities present in the intertidal zone of an unimpacted beach (a beach that did not suffer any significant oil spill) to degrade hydrocarbons was investigated. For that, laboratory-based microcosms (50-ml flasks) were set up with sandy beach sediment spiked with crude oil and incubated with local seawater for 15 days in the dark. Three bioremediation treatments were tested (biostimulation (BS), autochthonous bioaugmentation (AB), and combined treatment of biostimulation + bioaugmentation (BS + AB)) and the results were compared with natural attenuation (NA). Visual inspection showed clearly an oil solubility increase (confirmed by a higher hydrocarbons concentration in supernatant solutions) for all tested treatments when compared to NA. Significant degradation of the oil, shown by different profiles of petroleum hydrocarbons, was also observed for the different treatments particularly for BS + AB. Therefore, the microbial community of this unimpacted beach sediment could respond to an oil spill, degrading hydrocarbons. But to increase the natural attenuation pace, obtained results indicated that BS + AB is an appropriate approach for the bioremediation of beaches recently impacted by an oil spill. The autochthonous microbial cultures can be obtained "before" or "after" the contamination of the target site, being inoculated into the site right after it contamination.
CIMAR/CIIMAR-Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal. calmeida@ciimar.up.pt
C Marisa R
CM
Almeida
Izabela
I
Reis
M Nazaré
MN
Couto
Adriano A
AA
Bordalo
Ana P
AP
Mucha
D001493:Bathing Beaches; D001673:Biodegradation, Environmental; D015169:Colony Count, Microbial; D005410:Flame Ionization; D019015:Geologic Sediments / Q000382:microbiology*; D006838:Hydrocarbons / Q000378:metabolism*; D010578:Petroleum / Q000378:metabolism*; D059392:Petroleum Pollution / Q000517:prevention & control*; D011174:Portugal; D012623:Seawater / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D006838:Hydrocarbons; D010578:Petroleum; D014874:Water Pollutants, Chemical