Detection and characterization of a new betasatellite: variation in disease symptoms of tomato leaf curl Pakistan virus-India due to associated betasatellite.
A begomovirus and its associated betasatellites were amplified and sequenced from tobacco plants affected with leaf curl disease. The begomovirus was identified as a new strain of tomato leaf curl Pakistan virus (ToLCPKV), which is referred to here as ToLCPKV-India. A previously known betasatellite [tomato leaf curl Patna betasatellite (ToLCPaB)] and a new betasatellite were also found in leaf-curl-affected samples. The use of infectious clones of ToLCPKV-IN plus ToLCPaB for agroinoculation led to typical leaf curl, while ToLCPKV-IN together with the new betasatellite resulted in curling and chlorosis of leaves. Based on these disease symptoms, we propose to name the new betasatellite tobacco leaf chlorosis betasatellite (TbLChB).
National Agri-Food Biotechnology Institute, Mohali 160071, Punjab, India. jitsingh27281@gmail.com
Jitendra
J
Kumar
Sudhir P
SP
Singh
Abhinav
A
Kumar
Jawaid A
JA
Khan
Rakesh
R
Tuli
D053839:Begomovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004276:DNA, Satellite / Q000235:genetics / Q000302:isolation & purification*; D018551:Solanum lycopersicum / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D011995:Recombination, Genetic; D014026:Nicotiana / Q000821:virology*
D004276:DNA, Satellite