Towards an integrated biosensor array for simultaneous and rapid multi-analysis of endocrine disrupting chemicals.
In this paper we propose the construction and application of a portable multi-purpose biosensor array for the simultaneous detection of a wide range of endocrine disruptor chemicals (EDCs), based on the recognition operated by various enzymes and microorganisms. The developed biosensor combines both electrochemical and optical transduction systems, in order to increase the number of chemical species which can be monitored. Considering to the maximum residue level (MRL) of contaminants established by the European Commission, the biosensor system was able to detect most of the chemicals analysed with very high sensitivity. In particular, atrazine and diuron were detected with a limit of detection of 0.5nM, with an RSD% less than 5%; paraoxon and chlorpyrifos were revealed with a detection of 5 μM and 4.5 μM, respectively, with an RSD% less than 6%; catechol and bisphenol A were identified with a limit of detection of 1 μM and 35 μM respectively, with an RSD% less than 5%.
IC-CNR Istituto di Cristallografia, AdR1 Dipartimento Agroalimentare, Rome, Italy. viviana.scognamiglio@mlib.ic.cnr.it
Viviana
V
Scognamiglio
Italo
I
Pezzotti
Gianni
G
Pezzotti
Juan
J
Cano
Ivano
I
Manfredonia
Katia
K
Buonasera
Fabiana
F
Arduini
Danila
D
Moscone
Giuseppe
G
Palleschi
Maria Teresa
MT
Giardi
D001280:Atrazine / Q000032:analysis; D001559:Benzhydryl Compounds / Q000032:analysis; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D002396:Catechols / Q000032:analysis; D004237:Diuron / Q000032:analysis; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation; D052244:Endocrine Disruptors / Q000032:analysis*; D004867:Equipment Design; D004968:Estrogens, Non-Steroidal / Q000032:analysis; D006540:Herbicides / Q000032:analysis; D006801:Humans; D057230:Limit of Detection; D010261:Paraoxon / Q000032:analysis; D010636:Phenols / Q000032:analysis; D013997:Time Factors; D000098826:Bisphenol A Compounds
D001280:Atrazine; D001559:Benzhydryl Compounds; D002396:Catechols; D004237:Diuron; D052244:Endocrine Disruptors; D004968:Estrogens, Non-Steroidal; D006540:Herbicides; D010261:Paraoxon; D010636:Phenols; C034221:catechol; C006780:bisphenol A; D000098826:Bisphenol A Compounds