[Platelet link of homeostasis system at modeling of chronic stress situation].
Changes of thrombocyte morphological and functional status were investigated in rabbits (2,5-3 kg weight) by the emotional stress model that was reproduced through stimulation with irregular low-level electric current. Thrombocytes were purified from the venous blood with sequential centrifugation. Obtained material was investigated with electron microscope using luminous coloring agent acridine orange and syliconized glass. Glycosaminoglycans and Ca2+ noncompensated negative electric charge, the activity of ATP, alkaline phosphatase, adenylate cyclase and phosphodiesterase of cyclical nucleotides, monoamine oxidase were cytochemicaly revealed. Consequently morphological and functional equivalents of chronic stress destabilization effect on blood platelets were determined. Likewise membranotropic and receptor mediated mechanisms of this effect realization were analyzed.
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Gavrish
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Kilimnik
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Kindzerskaia
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000201:enzymology / Q000648:ultrastructure*; D002118:Calcium / Q000378:metabolism; D004558:Electric Stimulation / Q000009:adverse effects; D006025:Glycosaminoglycans / Q000378:metabolism; D006487:Hemostasis; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008954:Models, Biological; D008995:Monoamine Oxidase / Q000378:metabolism; D010974:Platelet Aggregation; D011817:Rabbits; D055672:Static Electricity; D013312:Stress, Physiological
D006025:Glycosaminoglycans; D008995:Monoamine Oxidase; D000469:Alkaline Phosphatase; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000251:Adenosine Triphosphatases; D000262:Adenylyl Cyclases; D002118:Calcium