Expression of IL-22, IL-22R and IL-23 in the peri-implant soft tissues of patients with peri-implantitis.
OBJECTIVE
The aim of this study was to compare the expression of interleukin (IL)-22, IL-22R and IL-23 in the peri-implant soft tissues between the peri-implantitis patient group (PG) and peri-implant healthy control group (HG).


METHODS
The tissues were collected from 12 peri-implantitis patients and eight peri-implant healthy controls. Immunohistochemistry (IHC) and real-time quantitative PCR (qPCR) were performed to analyse the gene expression of IL-22, IL-22R and IL-23p19 in peri-implant soft tissues in the PG and the HG group.


RESULTS
The IHC result showed that number of IL-22, IL-22R, and IL-23p19 positive cells increased in PG than in HG (P<0.05). The result of qPCR demonstrated that the expressions of IL-22 messenger RNA (mRNA) and IL-23p19 mRNA were significantly higher in the PG group compared to the HG group (P<0.05). Gene expression of IL-22R mRNA was higher in the PG group; however, there was no statistically significant difference between these two groups (P>0.05).


CONCLUSIONS
This study indicates that there is an increased expression level of IL-22 and IL-23 in patients with peri-implantitis, which may induce expression of related pro-inflammatory cytokines and may further have a crucial role in tissue repair and reconstruction in pathogenesis of peri-implantitis.
Department of Periodontics, West China College of Stomatology, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Zhenhua
Z
Luo
Hui
H
Wang
Zheng
Z
Sun
Wei
W
Luo
Yafei
Y
Wu
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D053760:Interleukin-23 Subunit p19 / Q000096:biosynthesis* / Q000235:genetics; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism*; D057873:Peri-Implantitis / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000235:genetics; D018709:Statistics, Nonparametric; D000097043:Interleukin-22
D053760:Interleukin-23 Subunit p19; D007378:Interleukins; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D000097043:Interleukin-22; C415369:interleukin-22 receptor