Serum protein levels following surgery in breast cancer patients: a protein microarray approach.
Surgery is the primary treatment for non-metastatic breast cancer. However,             the risk of early recurrence remains after surgical removal of the primary tumor.             Recurrence is suggested to result from hidden micrometastatic foci, which are             triggered to escape from dormancy by surgical resection of the primary tumor.             In this study, we focused on the differential impact of breast surgery on the             serum profiles of early breast cancer patients and healthy women. Serum samples             from invasive breast cancer patients, in situ carcinoma breast cancer patients             and healthy women were analyzed using reverse phase protein array technology.             Samples were collected prior to breast surgery and 24 h following breast surgery.             Both the expression level and the velocity of 42 serum proteins were quantified             and compared among groups. We found that surgery increased the concentration of             several proteins (CSF1, THSB2, IL6, IL7, IL16, FasL and VEGF-B) in the overall             population. Compared with healthy women and patients with non-invasive tumors,             invasive tumor patients exhibited higher preoperative levels of several serum             proteins, such as αFP, IFNβ1, VEGF-A, IL18, E-cadherin or CD31, and lower postoperative             levels of TNFα and IL5. Similarly, we detected significant surgery-induced changes             in the velocity of VEGF-A and IL16 accumulation in samples derived from invasive             breast cancer patients. In conclusion, breast surgery induced distinct changes             in the concentrations and dynamics of serum proteins in invasive breast cancer             patients compared with healthy women and non-invasive tumor patients.
Department of Medical Oncology, Hospital Universitario Virgen                     de la Victoria, Campus de Teatinos s/n, 29010 Malaga, Spain.
Luis G
LG
Perez-Rivas
Jose M
JM
Jerez
Cristina E
CE
Fernandez-De Sousa
Vanessa
V
de Luque
Cristina
C
Quero
Bella
B
Pajares
Leonardo
L
Franco
Alfonso
A
Sanchez-Muñoz
Nuria
N
Ribelles
Emilio
E
Alba
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood; D001798:Blood Proteins; D001943:Breast Neoplasms / Q000097:blood* / Q000473:pathology / Q000601:surgery; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011184:Postoperative Period; D040081:Protein Array Analysis; D040901:Proteomics; D055815:Young Adult
D014408:Biomarkers, Tumor; D001798:Blood Proteins