Evolutionary dynamism of the primate LRRC37 gene family.
Core duplicons in the human genome represent ancestral duplication modules shared by the majority of intrachromosomal duplication blocks within a given chromosome. These cores are associated with the emergence of novel gene families in the hominoid lineage, but their genomic organization and gene characterization among other primates are largely unknown. Here, we investigate the genomic organization and expression of the core duplicon on chromosome 17 that led to the expansion of LRRC37 during primate evolution. A comparison of the LRRC37 gene family organization in human, orangutan, macaque, marmoset, and lemur genomes shows the presence of both orthologous and species-specific gene copies in all primate lineages. Expression profiling in mouse, macaque, and human tissues reveals that the ancestral expression of LRRC37 was restricted to the testis. In the hominid lineage, the pattern of LRRC37 became increasingly ubiquitous, with significantly higher levels of expression in the cerebellum and thymus, and showed a remarkable diversity of alternative splice forms. Transfection studies in HeLa cells indicate that the human FLAG-tagged recombinant LRRC37 protein is secreted after cleavage of a transmembrane precursor and its overexpression can induce filipodia formation.
Dipartimento di Biologia, Università degli Studi di Bari Aldo Moro, Bari 70126, Italy.
Giuliana
G
Giannuzzi
Priscillia
P
Siswara
Maika
M
Malig
Tomas
T
Marques-Bonet
James C
JC
Mullikin
Mario
M
Ventura
Evan E
EE
Eichler
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002531:Cerebellum / Q000378:metabolism; D033481:Chromosomes, Mammalian / Q000235:genetics; D004247:DNA / Q000737:chemistry; D019143:Evolution, Molecular; D020440:Gene Duplication; D020869:Gene Expression Profiling; D016678:Genome / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D000090263:Leucine-Rich Repeat Proteins; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009928:Organ Specificity; D011323:Primates / Q000235:genetics*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics
D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins; D004247:DNA