Collagen with simvastatin promotes cell metabolism in osteoblast-like SaOS-2 cells.
BACKGROUND
Simvastatin (SMV) is one of the cholesterol-lowering pharmacological drugs. Recent studies demonstrate that it has a bone stimulatory effect. The present study was designed to investigate the effect of SMV along with collagen membrane on osteoblast-like SaOS-2 cells and also to standardize the dosage of SMV to be incorporated into the collagen membrane to achieve regeneration.


METHODS
SMV at doses of 0.5, 1, 1.5, and 2 mg was incorporated into the collagen membrane and cell metabolism was assessed by (3-[4,5-dimethylthiazolyl-2]-2,5-diphenyltetrazolium bromide) (MTT) assay for 24 h.


RESULTS
SMV enhanced cell metabolism dose dependently at 24-h time and the maximum effect was obtained at a concentration of 1.5 mg of SMV.


CONCLUSIONS
These results indicate that collagen with 1.5 mg SMV exhibits positive effect on cell metabolism of human osteoblast-like SaOS-2 cells.
Department of Periodontology and Oral Implantology, JKK Nataraja Dental College and Hospital, Komarapalyam, Namakkal Dist, India.
Thanga Kumaran
TK
Suthanthiran
Sugumari
S
Elavarasu
Devisree
D
Naveen
Umamaheswari
U
Nagarathinam
K V
KV
Arun
N
N
Srinivasan