Visible spectrophotometric method for amiodarone.
Amiodarone is an antiarrhythmic agent used for various types of tachyarrhythmia, both ventricular and supraventricular (atrial) arrhythmia. A spectrophotometric method for the assay of amiodarone was established. Based on the reduction of potassium ferricyanide in hydrochloric acid medium to potassium ferrocyanide forming a blue colored complex ferric ferrocyanide with Fe (III) ions. The compound was most stable in a mixture of ethylic alcohol and water (2:1, v/v) and it had an absorption maximum at 725 nm. The data were according to the Lambert-Beer Law in the concentration range of 0.5-5.0 microg/sample: correlation and coefficient R = 0.99977, R2 = 0.999541, slope of the line 0.12775, intercept 0.042077. The detection limit (DL) was 0.1032 microg/sample and the quantification limit (QL) 0.344 microg/ sample.
Discipline of Pharmaceutical Technology, School of Pharmacy, University of Medicine and Pharmacy, "Grigore T. Popa", Iasi.
Andreea
A
Bosînceanu
Graţiela
G
Popa
Gladiola
G
Tântaru
Iuliana
I
Popovici
D000042:Absorption; D000638:Amiodarone / Q000032:analysis* / Q000737:chemistry; D000889:Anti-Arrhythmia Agents / Q000032:analysis* / Q000737:chemistry; D004396:Coloring Agents / Q000138:chemical synthesis; D000431:Ethanol / Q000737:chemistry; D005292:Ferricyanides / Q000737:chemistry; D005295:Ferrocyanides / Q000138:chemical synthesis; D006851:Hydrochloric Acid / Q000737:chemistry; D057230:Limit of Detection; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014867:Water
D000889:Anti-Arrhythmia Agents; D004396:Coloring Agents; D005292:Ferricyanides; D005295:Ferrocyanides; D014867:Water; D000431:Ethanol; C031835:potassium ferrocyanide; D000638:Amiodarone; D006851:Hydrochloric Acid; C000170:ferric ferrocyanide; C028033:potassium ferricyanide