A sensitive H2O2 assay based on dumbbell-like PtPd-Fe3O4 nanoparticles.
Dumbbell-like Pt(48)Pd(52)-Fe(3)O(4) nanoparticles are synthesized and functionalized with oleylamine-polyethyleneglycol to serve as an efficient catalyst for H(2)O(2) reduction and tetramethylbenzidine (TMB) oxidation in biological solutions. The Pt(48)Pd(52)-Fe(3)O(4)/TMB kit is even more active than the natural enzyme for H(2)O(2) detection with a detection limit reaching 2 μM, and is successfully used to quantitatively monitor the extracellular H(2)O(2) generated by neutrophils.
Department of Chemistry, Brown University, Providence, RI 02912, USA.
Xiaolian
X
Sun
Shaojun
S
Guo
Chun-Shiang
CS
Chung
Wenlei
W
Zhu
Shouheng
S
Sun
D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D002460:Cell Line; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D010165:Palladium / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*
D058185:Magnetite Nanoparticles; D010984:Platinum; D010165:Palladium; D006861:Hydrogen Peroxide