Western blot: technique, theory, and trouble shooting.
Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. This paper will attempt to explain the technique and theory behind western blot, and offer some ways to troubleshoot.
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada.
Tahrin
T
Mahmood
Ping-Chang
PC
Yang