Oligonol-induced degradation of perilipin 1 is regulated through lysosomal degradation machinery.
The results obtained from our previous study showed that the addition of a lychee fruit-derived low molecular form of polyphenol, Oligonol, provoked higher levels of lipolytic activity via the degradation of perilipin 1 in primary rat adipocytes. In the current study, we investigated the possible mechanisms by which Oligonol could promote the degradation of perilipin 1 protein. The addition of Oligonol caused the degradation of GFP-tagged perilipin 1 in a time-dependent manner. Meanwhile, the co-addition of Oligonol and NH4CI, a lysosome inhibitor, failed to promote the degradation of perilipin 1, while the co-addition of Oligonol and MG132, a proteasome inhibitor, induced a reduction in the levels of perilipin 1. These results suggest that the Oligonol-induced degradation of perilipin 1 is regulated via a lysosome-dependent mechanism.
Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine, Tokyo 181-8611, Japan. junetsu@ks.kyorin-u.ac.jp
Junetsu
J
Ogasawara
Kentaro
K
Kitadate
Hiroshi
H
Nishioka
Hajime
H
Fujii
Takuya
T
Sakurai
Takako
T
Kizaki
Tetsuya
T
Izawa
Hitoshi
H
Ishida
Hideki
H
Ohno
D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008066:Lipolysis; D008247:Lysosomes / Q000378:metabolism*; D000070778:Perilipin-1; D010636:Phenols / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism*; D051381:Rats
D002352:Carrier Proteins; C000602978:PLIN1 protein, human; D000070778:Perilipin-1; D010636:Phenols; D010750:Phosphoproteins; C000602981:Plin1 protein, rat; C514283:oligonol; D002392:Catechin