A FRET enzyme-based probe for monitoring hydrogen sulfide.
Fluorescently labeled cobalt peptide deformylase (Co-PDF) can be efficiently used as a fluorescence-resonance-energy-transfer-based sensing device for hydrogen sulfide (H(2)S). The proof of concept of our sensor system is substantiated by spectroscopic, structural, and theoretical results. Monohydrogen sulfide coordination to Co-PDF and Ni-PDF was verified by X-ray crystallography. Density functional theory calculations were performed to gain insight into the characteristics of the coordination adduct between H(2)S and the cobalt cofactor in Co-PDF.
Dipartimento di Chimica e Biologia, Università degli Studi di Salerno, via Ponte Don Melillo, I-84084 Fisciano (Sa), Italy. mstriane@unisa.it
Maria
M
Strianese
Gottfried J
GJ
Palm
Stefano
S
Milione
Olaf
O
Kühl
Winfried
W
Hinrichs
Claudio
C
Pellecchia
D000581:Amidohydrolases / Q000737:chemistry* / Q000378:metabolism; D003035:Cobalt / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000201:enzymology*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry*; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000378:metabolism; D008958:Models, Molecular
D005456:Fluorescent Dyes; D003035:Cobalt; D000581:Amidohydrolases; C084507:peptide deformylase; D006862:Hydrogen Sulfide