Monosaccharide precursors for boosting chondroitin-like capsular polysaccharide production.
Chondroitin sulfate is a well-known bioactive molecule, widely used as an anti-osteoarthritis drug, that is nowadays mainly produced by animal tissue sources with unsafe extraction procedures. Recent studies have explored an integrated biotechnological-chemical strategy to obtain a chondroitin sulfate precursor from Escherichia coli K4 capsular polysaccharide, demonstrating the influence of environmental and growth conditions on capsule synthesis. In this research work, the flexibility of the strain biosynthetic machinery was investigated to enhance the K4 capsular polysaccharide production by supplementing the growth medium with the monosaccharides (glucuronic acid, galactosamine and fructose) that constitute the chain. Shake flask experiments were performed by adding the sugars singularly or together, by testing monosaccharide different concentrations and times of addition and by observing the bacterial sugar consumption. A K4 capsular polysaccharide production enhancement, compared to the control, was observed in all cases of supplementation and, in particular, significant 68 and 57 % increases were observed when adding 0.385 mM glucuronic acid plus galactosamine or 0.385 mM fructose, respectively. Increased expression levels of the gene kfoC, coding for a K4 polymerase, evaluated in different growth conditions, confirmed the results at the molecular level. Furthermore, batch fermentations, performed in lab-scale reactors (2 L), allowed to double the K4 capsular polysaccharide production values obtained in shake flask conditions, by means of a strict control of the growth parameters.
Centro Servizi di Ateneo "Grandi Apparecchiature", Second University of Naples, Via De Crecchio 7, 80138 Naples, Italy.
Odile Francesca
OF
Restaino
Irene
I
di Lauro
Donatella
D
Cimini
Elisabetta
E
Carlino
Mario
M
De Rosa
Chiara
C
Schiraldi
D016667:Bacterial Capsules / Q000378:metabolism*; D002809:Chondroitin Sulfates / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D005990:Glycerol / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism*
D003470:Culture Media; D029968:Escherichia coli Proteins; D009005:Monosaccharides; C063323:capsular polysaccharide K4; D002809:Chondroitin Sulfates; D004259:DNA-Directed DNA Polymerase; D005990:Glycerol