Host membrane proteins involved in the replication of tobamovirus RNA.
Eukaryotic positive-strand RNA viruses replicate their genomes in membrane-bound replication complexes composed of viral replication proteins and negative-strand RNA templates. These replication proteins are programmed to exhibit RNA polymerase and other replication-related activities only in replication complexes to avoid inducing double-stranded RNA-mediated host defenses. Host membrane components (e.g. proteins and lipids) should play important roles in the activation of replication proteins. Two host membrane proteins are components of the replication complex and activate the replication proteins of tobamoviruses. Interaction analyses using deletion mutants constructed based on structural information suggest a conformational change in replication proteins during the formation of a protein complex with RNA 5'-capping activity.
Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan.
Kazuhiro
K
Ishibashi
Shuhei
S
Miyashita
Etsuko
E
Katoh
Masayuki
M
Ishikawa
D054884:Host-Pathogen Interactions; D046911:Macromolecular Substances / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D017898:Tobamovirus / Q000472:pathogenicity / Q000502:physiology*; D014779:Virus Replication
D046911:Macromolecular Substances; D008565:Membrane Proteins; D012367:RNA, Viral