Neuronal growth and differentiation on biodegradable membranes.
Semipermeable polymeric membranes with appropriate morphological, physicochemical and transport properties are relevant to inducing neural regeneration. We developed novel biodegradable membranes to support neuronal differentiation. In particular, we developed chitosan, polycaprolactone and polyurethane flat membranes and a biosynthetic blend between polycaprolactone and polyurethane by phase-inversion techniques. The biodegradable membranes were characterized in order to evaluate their morphological, physicochemical, mechanical and degradation properties. We investigated the efficacy of these different membranes to promote the adhesion and differentiation of neuronal cells. We employed as model cell system the human neuroblastoma cell line SHSY5Y, which is a well-established system for studying neuronal differentiation. The investigation of viability and specific neuronal marker expression allowed assessment that the correct neuronal differentiation and the formation of neuronal network had taken place in vitro in the cells seeded on different biodegradable membranes. Overall, this study provides evidence that neural cell responses depend on the nature of the biodegradable polymer used to form the membranes, as well as on the dissolution, hydrophilic and, above all, mechanical membrane properties. PCL-PU membranes exhibit mechanical properties that improve neurite outgrowth and the expression of specific neuronal markers.
Institute of Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Rende, CS, Italy.
Sabrina
S
Morelli
Antonella
A
Piscioneri
Antonietta
A
Messina
Simona
S
Salerno
Mohamed B
MB
Al-Fageeh
Enrico
E
Drioli
Loredana
L
De Bartolo
D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008567:Membranes, Artificial; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009443:Neuritis / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D011140:Polyurethanes; C016240:polycaprolactone; D048271:Chitosan