Molecular epidemiology of Malassezia globosa and Malassezia restricta in Sudanese patients with pityriasis versicolor.
Pityriasis versicolor is a superficial infection of the stratum corneum caused by Malassezia yeasts. The cutaneous Malassezia                 globosa and Malassezia restricta in Sudanese patients with pityriasis versicolor were elucidated using a molecular-based, culture-independent method and compared with that in healthy individuals. Scale samples were collected by applying an Opsite™ transparent dressing to lesional and non-lesional sites on 29 Sudanese patients with pityriasis versicolor and 54 healthy individuals. Malassezia DNA was extracted directly from the samples. The overall level of colonization by Malassezia globosa and Malassezia restricta was analyzed by real-time PCR using a TaqMan probe. The overall level of colonization by Malassezia at the lesional sites was higher than that at the non-lesional sites for all body sites, including the face, neck, cheeks, and trunk (2.7- to 6.0-fold increase). Both M. globosa and M. restricta were detected in patients and healthy individuals. However, M. globosa predominated at lesional sites, whereas the level of colonization by both species was similar in healthy individuals.
Department of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan. saadmutaz74@yahoo.in
M
M
Saad
T
T
Sugita
H
H
Saeed
A
A
Ahmed
D000328:Adult; D000368:Aged; D015169:Colony Count, Microbial; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008290:Malassezia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D060888:Real-Time Polymerase Chain Reaction; D013397:Sudan / Q000453:epidemiology; D014010:Tinea Versicolor / Q000453:epidemiology* / Q000382:microbiology*; D055815:Young Adult
D004271:DNA, Fungal