Alternative ground states enable pathway switching in biological electron transfer.
Electron transfer is the simplest chemical reaction and constitutes the basis of a large variety of biological processes, such as photosynthesis and cellular respiration. Nature has evolved specific proteins and cofactors for these functions. The mechanisms optimizing biological electron transfer have been matter of intense debate, such as the role of the protein milieu between donor and acceptor sites. Here we propose a mechanism regulating long-range electron transfer in proteins. Specifically, we report a spectroscopic, electrochemical, and theoretical study on WT and single-mutant Cu(A) redox centers from Thermus thermophilus, which shows that thermal fluctuations may populate two alternative ground-state electronic wave functions optimized for electron entry and exit, respectively, through two different and nearly perpendicular pathways. These findings suggest a unique role for alternative or "invisible" electronic ground states in directional electron transfer. Moreover, it is shown that this energy gap and, therefore, the equilibrium between ground states can be fine-tuned by minor perturbations, suggesting alternative ways through which protein-protein interactions and membrane potential may optimize and regulate electron-proton energy transduction.
Facultad de Ciencias Bioquímicas y Farmacéuticas, Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Rosario, S2002LRK Rosario, Argentina.
Luciano A
LA
Abriata
Damián
D
Álvarez-Paggi
Gabriela N
GN
Ledesma
Ninian J
NJ
Blackburn
Alejandro J
AJ
Vila
Daniel H
DH
Murgida
D004579:Electron Transport; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D016963:Thermus thermophilus / Q000378:metabolism*; D056928:X-Ray Absorption Spectroscopy