Complete sequence of pJIE186-2, a plasmid carrying multiple virulence factors from a sequence type 131 Escherichia coli O25 strain.
The complete sequence of a 137-kb plasmid, pJIE186-2, from a sequence type 131 (ST131) Escherichia coli strain was determined. pJIE186-2 contained IncF replicons (FIB, FIIA, and FIAΔ), an incomplete conjugative region, and multiple virulence factors (sitABCD, iucABCD-iutA, iroCDEN, etsABC, hlyF, iss, ompT, and vagCD) but no antimicrobial resistance genes. The host strain also had another plasmid, pJIE186-1, carrying multiple resistance genes. The two plasmids conferred selective advantages for the host strain, contributing to the recent emergence of ST131 E. coli.
Center of Infectious Diseases, West China Hospital, Sichuan University, and Division of Infectious Diseases, the State Key Laboratory of Biotherapy, Sichuan University, Chengdu, China. zongzhiy@scu.edu.cn
Zhiyong
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Zong
D001315:Australia; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D012093:Replicon; D012641:Selection, Genetic; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics*
D037521:Virulence Factors