The O-specific polysaccharide structure and gene cluster of serotype O:12 of the Yersinia pseudotuberculosis complex, and the identification of a novel L-quinovose biosynthesis gene.
A major virulence factor for Yersinia pseudotuberculosis is lipopolysaccharide, including O-polysaccharide (OPS). Currently, the OPS based serotyping scheme for Y. pseudotuberculosis includes 21 known O-serotypes, with genetic and structural data available for 17 of them. The completion of the OPS structures and genetics of this species will enable the visualization of relationships between O-serotypes and allow for analysis of the evolutionary processes within the species that give rise to new serotypes. Here we present the OPS structure and gene cluster of serotype O:12, thus adding one more to the set of completed serotypes, and show that this serotype is present in both Y. pseudotuberculosis and the newly identified Y. similis species. The O:12 structure is shown to include two rares ugars: 4-C[(R)-1-hydroxyethyl]-3,6-dideoxy-D-xylo-hexose(D-yersiniose) and 6-deoxy-L-glucopyranose (L-quinovose).We have identified a novel putative guanine diphosphate(GDP)-L-fucose 4-epimerase gene and propose a pathway for the synthesis of GDP-L-quinovose, which extends the known GDP-L-fucose pathway.
Department of Chemical Sciences,  University Federico II of Naples, Naples,  Italy.
Cristina
C
De Castro
Johanna J
JJ
Kenyon
Monica M
MM
Cunneen
Antonio
A
Molinaro
Otto
O
Holst
Mikael
M
Skurnik
Peter R
PR
Reeves
D001426:Bacterial Proteins / Q000235:genetics; D002238:Carbohydrate Epimerases / Q000235:genetics; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D006601:Hexoses / Q000737:chemistry; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry*; D015011:Yersinia pseudotuberculosis / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics
D001426:Bacterial Proteins; D006601:Hexoses; D019081:O Antigens; C041266:yersiniose; D003847:Deoxyglucose; D002238:Carbohydrate Epimerases; C037904:6-deoxyglucose