Enhancement of fatty acid-based polyurethanes cytocompatibility by non-covalent anchoring of chondroitin sulfate.
For tissue engineering purpose biopolymer chondroitin sulfate (CS), one of the major components of cartilage and bone extracellular matrix, was immobilized onto the surface of amino-functionalized polyurethane (PU) films derived from naturally occurring oleic and 10-undecenoic acids. The amino-functionalized PUs were prepared by aminolysis with 1,6-hexamethylenediamine of synthesized PUs containing methyl ester groups. FTIR-ATR, XPS, SEM, and water contact angle measurements were used to confirm the surface changes at each step of treatment, both in morphologies and chemical composition. Cytotoxicity and cell morphology analysis using osteoblast cell line MG63 showed that PU-CS films are suitable materials for cell growth, spreading, and differentiation.
Department of Analytical and Organic Chemistry, Rovira i Virgili University, Campus Sescelades, C/Marcel.lí Domingo s/n, 43007 Tarragona, Spain.
Rodolfo J
RJ
González-Paz
Gerard
G
Lligadas
Juan C
JC
Ronda
Marina
M
Galià
Ana M
AM
Ferreira
Francesca
F
Boccafoschi
Gianluca
G
Ciardelli
Virginia
V
Cádiz
D001672:Biocompatible Materials / Q000737:chemistry*; D045744:Cell Line, Tumor; D002809:Chondroitin Sulfates / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D009829:Oleic Acids / Q000737:chemistry; D056951:Photoelectron Spectroscopy; D011140:Polyurethanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D014476:Undecylenic Acids; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D009829:Oleic Acids; D011140:Polyurethanes; D014476:Undecylenic Acids; D014867:Water; D002809:Chondroitin Sulfates; C538763:undecylenic acid