Living target of Ce(III) action on horseradish cells: proteins on/in cell membrane.
Positive and negative effects of rare earth elements (REEs) in life have been reported in many papers, but the cellular mechanisms have not been answered, especially the action sites of REEs on plasma membrane are unknown. Proteins on/in the plasma membrane perform main functions of the plasma membrane. Cerium (Ce) is the richest REEs in crust. Thus, the interaction between Ce(III) and the proteins on/in the plasma membrane, the morphology of protoplast, and the contents of nutrient elements in protoplast of horseradish were investigated using the optimized combination of the fluorescence microscopy, fluorescence spectroscopy, circular dichroism, scanning electron microscopy, and X-ray energy dispersive spectroscopy. It was found that Ce(III) at the low concentrations (10, 30 μM) could interact with proteins on/in the plasma membrane of horseradish, leading to the improvement in the structure of membrane proteins and the plasma membrane, which accelerated the intra-/extra-cellular substance exchange and further promoted the development of cells. When horseradish was treated with Ce(III) at the high concentrations (60, 80 μM), Ce(III) also could interact with the proteins on/in the plasma membrane of horseradish, leading to the destruction in the structure of membrane proteins and the plasma membrane. These effects decelerated the intra-/extra-cellular substance exchange and further inhibited the development of cells. Thus, the interaction between Ce(III) and proteins on/in the plasma membrane in plants was an important reason of the positive and negative effects of Ce(III) on plants. The results would provide some references for understanding the cellular effect mechanisms of REEs on plants.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Guangmei
G
Yang
Zhaoguo
Z
Sun
Xiaofen
X
Lv
Yunyun
Y
Deng
Qing
Q
Zhou
Xiaohua
X
Huang
D000042:Absorption / Q000187:drug effects; D031215:Armoracia / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002563:Cerium / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology*; D059165:Hormesis; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D055550:Protein Stability / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects; D011523:Protoplasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D036226:Seedlings / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D014131:Trace Elements / Q000378:metabolism
D008565:Membrane Proteins; D010940:Plant Proteins; D014131:Trace Elements; D002563:Cerium