Effect of Jagged1 on the proliferation and migration of colon cancer cells.
The aim of this study was to determine the effect of Jagged1 on the proliferation and migration of colon cancer cells. HT-29 colorectal carcinoma cells were transfected with adenovirus carrying either the Jagged1 gene (Ad-Jagged1) or Jagged1 small interfering RNA (siRNA) (Ad-si/hJagged1). The protein was detected using western blot analysis. The proliferation of cells was observed by assessing the incorporation of [(3)H]-thymidine. The migration of cells was detected using a modified Boyden chamber assay. The level of Jagged1 protein increased by 2.8-fold with Ad-Jagged1 transfection and decreased by 70% with Ad-si/hJagged1 transfection. Proliferation, migration and p-Akt protein expression were enhanced in the Ad-Jagged1-transfected group compared to that in the non-transfected control group. In addition, the proliferation, migration and phosphorylated Akt protein expression were reduced in the Ad-si/hJagged1-transfected group compared to that in the non-transfected control group. The Jagged1 protein may promote proliferation and migration of colon cancer cells, which may be related to its effect on Akt phosphorylation.
Department of Oncology, The General Hospital of Chengdu Military Region, Chengdu 610083,  P.R. China.
Yong
Y
Tan
Jingjing
J
Peng
Dong
D
Wei
Peng
P
Chen
Yu
Y
Zhao