Molecular characterisation of methicillin-susceptible and methicillin-resistant Staphylococcus aureus in inpatients and outpatients in Bosnia and Herzegovina.
The aim of this study was to investigate the genetic background of methicillin-susceptible (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) obtained from clinical specimens of inpatients and outpatients. Methicillin resistance was confirmed by the presence of the mecA gene by PCR. The genetic characterisation was performed using spa typing and the algorithm based upon repeat pattern (BURP). Staphylococcus aureus was isolated from 68 and 79 inpatient and outpatient samples, 31 (46 %) and 14 (18 %) of which were MRSA, respectively. Among 37 inpatients and 65 outpatients with MSSA, 22 and 38 spa types were clustered into seven and eight spa-CCs, respectively. The main MSSA spa-CC of inpatients and outpatients was spa-CC015 (multilocus sequence typing (MLST) CC45). Most MRSA were associated with spa-CC355/595 (MLST CC152). MRSA-related background was found in 32 % of inpatients and 43 % of outpatients with MSSA, suggesting that MRSA did not arise from predominant MSSA clones.
Department for Laboratory Diagnostics, Cantonal Public Health Institute Zenica, Fra Ivana Jukica 2, 72000, Zenica, Bosnia and Herzegovina. selma_kamb@yahoo.com
Selma
S
Uzunović-Kamberović
Michelle I A
MI
Rijnders
Ellen E
EE
Stobberingh
Amir
A
Ibrahimagić
Farah
F
Kamberović
Tatjana
T
Ille
D000293:Adolescent; D000328:Adult; D000368:Aged; D000465:Algorithms; D000553:Ambulatory Care; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D017522:Bosnia and Herzegovina; D002648:Child; D002675:Child, Preschool; D003428:Cross Infection / Q000188:drug therapy / Q000382:microbiology*; D003430:Cross-Sectional Studies; D005260:Female; D056426:Genetic Loci / Q000235:genetics; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008712:Methicillin / Q000627:therapeutic use*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D046915:Penicillin-Binding Proteins; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000453:epidemiology / Q000382:microbiology*; D013205:Staphylococcal Protein A / Q000235:genetics; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics*; D055815:Young Adult
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D046915:Penicillin-Binding Proteins; D013205:Staphylococcal Protein A; C487789:mecA protein, Staphylococcus aureus; D008712:Methicillin