R26R-GR: a Cre-activable dual fluorescent protein reporter mouse.
Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.
Graduate Institute of Medical Genomics and Proteomics, National Taiwan University College of Medicine, Taipei, Taiwan.
You-Tzung
YT
Chen
Ming-Shian
MS
Tsai
Tsung-Lin
TL
Yang
Amy Tsu
AT
Ku
Ke-Han
KH
Huang
Cheng-Yen
CY
Huang
Fu-Ju
FJ
Chou
Hsiang-Hsuan
HH
Fan
Jin-Bon
JB
Hong
Shuo-Ting
ST
Yen
Wei-Le
WL
Wang
Chang-Ching
CC
Lin
Yu-Chen
YC
Hsu
Kang-Yi
KY
Su
I-Chang
IC
Su
Chuan-Wei
CW
Jang
Richard R
RR
Behringer
Rebecca
R
Favaro
Silvia K
SK
Nicolis
Chung-Liang
CL
Chien
Shu-Wha
SW
Lin
I-Shing
IS
Yu
D000483:Alleles; D000818:Animals; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D019426:Integrases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008938:Mitosis / Q000235:genetics / Q000502:physiology
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C045073:Cre recombinase; D019426:Integrases