[Dynamic observation on MDA, iNOS and antisuperoxide anion in the liver of rats induced by acute nickel carbonyl poisoning].
OBJECTIVE
To investigate the level of malondiadehyde (MDA), antisuperoxide anion and inducible nitric oxide synthase (iNOS) in liver of rats poisoned by nickel carbonyl in order to discuss the mechanism of acute nickel carbonyl poisoning.


METHODS
Healthy SD rats were intoxicated acutely by different concentrations of nickel carbonyl (20, 135 and 250mg/m3 for low, middle and high dose groups, respectively). SD rats inhaled by chlorine (250mg/m3 for chlorine group) were used as positive control group and other healthy SD rats as normal control group. Liver of animals was taken at different time points after exposure. The levels of MDA, iNOS and antisuperoxide anion were detected by biochemical assay.


RESULTS
The contents of MDA and antisuperoxide anion in the liver of high dose group were significantly higher than that of other exposed groups and control group (P < 0.01). The contents of iNOS in middle and high dose group were higher than that in low dose group and control group (P < 0.05).


CONCLUSIONS
The oxidative damage in the liver of SD rats could be induced by carbonyl nickel in air with increasing concentrations and in an obvious dose-response relationships.
Key Laboratory of Preclinical Study of New Drug of Gansu Province, Lanzhou University, Lanzhou 730000, China. guyanymf@163.com
Mingfeng
M
Yan
Qiuying
Q
Wang
Ning
N
Wang
Xijiang
X
Wu
Qin
Q
Luo
Xiaoqiang
X
Xuan
Xiufang
X
Ren
Xiaohuai
X
Wang
Guangwei
G
Qi
Ning
N
Cheng
D000818:Animals; D000838:Anions; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects*; D009942:Organometallic Compounds / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000838:Anions; D009942:Organometallic Compounds; C005303:nickel carbonyl; D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat