Modulatory effect of lycopene on deltamethrin-induced testicular injury in rats.
Aim of the current study was to investigate the ability of deltamethrin to induce testicular injury in rats and its possible attenuation with lycopene. Rats were divided into three groups: Group I (DEL) received deltamethrin, 5 mg/kg b.w./day orally, in corn oil. Group II (DEL + Lyc) received oral dose of lycopene (4 mg/kg b.w./day) in corn oil concurrently with deltamethrin following the same regimen as in group I. Group III (Control) received appropriate volume of corn oil. After 4 weeks, deltamethrin-treated rats showed decreased body weight, serum testosterone, luteinizing hormone and follicle-stimulating hormone levels. Testicular total oxidant capacity (TOC), nitrite/nitrate (NOx), poly (ADP-ribose) polymerase (PARP), and DNA damage were significantly increased. RT-PCR demonstrated significant up-regulation in testicular mRNA for glutathione-S-transferase and heat-shock protein-70 (HSP-70), whereas steroidogenic acute regulatory (StAR) protein was down-regulated after deltamethrin exposure. Lycopene was able to restore body weight, serum testosterone, StAR mRNA, TOC, NOx levels, and PARP activity with significant decrease in HSP-70 mRNA, and DNA damage. In conclusion, lycopene was able to counteract the deleterious effect of deltamethrin.
Biochemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Eini Street, Cairo, 11562, Egypt. manalfouad1@yahoo.com
Manal F
MF
Ismail
Hanaa M
HM
Mohamed
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D002338:Carotenoids / Q000494:pharmacology / Q000627:therapeutic use*; D004249:DNA Damage / Q000187:drug effects; D015536:Down-Regulation; D005640:Follicle Stimulating Hormone / Q000097:blood; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D000077276:Lycopene; D008297:Male; D009570:Nitriles / Q000633:toxicity; D009589:Nitrogen Oxides / Q000097:blood; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011722:Pyrethrins / Q000633:toxicity; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013733:Testicular Diseases / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D015854:Up-Regulation; D000098682:Steroidogenic Acute Regulatory Protein
D000975:Antioxidants; D018840:HSP70 Heat-Shock Proteins; D009570:Nitriles; D009589:Nitrogen Oxides; D010750:Phosphoproteins; D011722:Pyrethrins; D012333:RNA, Messenger; D000098682:Steroidogenic Acute Regulatory Protein; C017180:decamethrin; D002338:Carotenoids; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D011065:Poly(ADP-ribose) Polymerases; D005982:Glutathione Transferase; D000077276:Lycopene