Hepatitis E virus genotype 4 isolated from a patient with liver failure: full-length sequence analysis showing potential determinants of virus pathogenesis.
The full-length genome sequence of a genotype 4 strain of Hepatitis E virus (HEV) (CHN-NJ-H2011) from a patient (in Nanjing, China) with liver failure has been determined. Phylogenetic analysis showed that CHN-NJ-H2011 belongs to genotype 4, subtype 4h. Comparative sequence analysis carried out on a 301-bp fragment of ORF2 showed that CHN-NJ-H2011 shares high nucleotide sequence identity (94.3-94.7 %) with porcine viruses (ch-shsw1 and Ch-estw2) isolated in the same geographical region, pointing to the strong possibility of zoonotic transmission of HEV genotype 4. A broader comparison with other genotype 4 isolates revealed 12 unique amino acid substitutions in ORF1 and three in ORF2 that might serve as signatures of disease severity for genotype 4 HEV infection.
Department of Microbiology, School of Basic Medical Sciences, Peking University Infectious Disease Center, Peking University, Beijing 100191, China.
Qiuning
Q
Bu
Xiaojuan
X
Wang
Ling
L
Wang
Peng
P
Liu
Jiabao
J
Geng
Maorong
M
Wang
Jian
J
Han
Yonghong
Y
Zhu
Hui
H
Zhuang
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000368:Aged; D001483:Base Sequence; D002681:China; D005243:Feces / Q000821:virology; D005838:Genotype; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D006801:Humans; D017093:Liver Failure / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny
D020413:3' Untranslated Regions; D020121:5' Untranslated Regions