Expression pattern of L-FABP gene in different tissues and its regulation of fat metabolism-related genes in duck.
Liver fatty acid binding protein (L-FABP) is a member of intracellular lipid-binding proteins responsible for the transportation of fatty acids. The expression pattern of duck L-FABP mRNA was examined in this study by quantitative RT-PCR. The results showed that duck L-FABP gene was expressed in many tissues, including heart, lung, kidney, muscle, ovary, brain, intestine, stomach and adipocyte tissues, and highly expressed in liver. Several lipid metabolism-related genes were selected to detect the regulation of L-FABP in duck. The expression of L-FABP and lipoprotein lipase was promoted by oleic acid. The L-FABP knockdown decreased the expression levels of peroxisome proliferator-activated receptor α (PPARα), fatty acid synthase and lipoprotein lipase by 61.1, 42.3 and 53.7 %, respectively (P < 0.05), but had no influences on the mRNA levels of PPARγ and leptin receptor. L-FABP might function through the PPARα to regulate the fat metabolism-related gene expression and play important roles in lipid metabolism in duck hepatocytes.
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Hangzhou 310021, Zhejiang, China.
Jun
J
He
Yong
Y
Tian
Jinjun
J
Li
Junda
J
Shen
Zhengrong
Z
Tao
Yan
Y
Fu
Dong
D
Niu
Lizhi
L
Lu
D000818:Animals; D004372:Ducks / Q000235:genetics* / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D050356:Lipid Metabolism / Q000235:genetics*; D008297:Male; D019301:Oleic Acid / Q000494:pharmacology; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger; D019301:Oleic Acid