Biomarker detection of rhGH doping: an excretion study.
The purpose of this research is to validate the biomarker-based approach for the detection of doping with recombinant human growth hormone (rhGH) in sport. The GH-2000 project proposed an indirect method for the detection of exogenously administered growth hormone (GH) based on the measurement of the GH-dependent markers: insulin-like growth factor-I (IGF-I) and Type III pro-collagen (P-III-P). These markers rise in a dose-dependent manner after GH application. In this study, the concentrations of IGF-I, IGF-BP3, and P-III-P in serum were determined to provide further incentives for the implementation of this detection assay in modern anti-doping programmes. This paper reports on an administration study of rhGH involving 25 Chinese male volunteers at a dose of 0.1 IU /kg/day for a continuous 14-day period. We observed that the serum IGF-I concentration increased rapidly in the rhGH treatment group and showed significantly higher levels compared to baseline between days 4 and day 16 after administration. Although the response of P-III-P to rhGH administration was delayed compared to the IGF-I axis, the P-III-P concentration remained increased for a longer period (from day 4 to day 28). Statistical analysis was carried out to establish a discriminant formula with Statistical Product and Service Solutions (SPSS) concluding that the biomarker methodology is valid and universally applicable.
China Anti-Doping Agency, Beijing, 100029, China.
Jing
J
Jing
Xinmiao
X
Zhou
Chunji
C
He
Lisi
L
Zhang
Sheng
S
Yang
Youxuan
Y
Xu
Minhao
M
Xie
Yi
Y
Yan
Hao
H
Su
Moutian
M
Wu
D000328:Adult; D015415:Biomarkers / Q000097:blood; D004300:Doping in Sports; D004311:Double-Blind Method; D019382:Human Growth Hormone / Q000008:administration & dosage* / Q000097:blood*; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000097:blood*; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D008297:Male; D010446:Peptide Fragments / Q000097:blood*; D011347:Procollagen / Q000097:blood*; D011994:Recombinant Proteins / Q000097:blood; D015813:Substance Abuse Detection / Q000379:methods; D055815:Young Adult
D015415:Biomarkers; D018972:Insulin-Like Growth Factor Binding Protein 3; D010446:Peptide Fragments; D011347:Procollagen; D011994:Recombinant Proteins; C041876:procollagen Type III-N-terminal peptide; D019382:Human Growth Hormone; D007334:Insulin-Like Growth Factor I