The use of angiogenic-antimicrobial agents in experimental wounds in animals: problems and solutions.
A topical combination (silvathymosin) of natural proangiogeneic protein thymosin β4 (Tβ4) and antimicrobial silver sulfadiazine was hypothesized to promote the healing of large, full-thickness, clean or infected wounds in rats. Silvathymosin showed the fastest wound healing (85%) followed by silver sulfadiazine (84%) and Tβ4 (72%). In the infected groups, the healing pattern was different, as Tβ4 and silvathymosin groups did not show similar wound healing. Wound histopathology and VEGF and KI67 immunohistochemical assessment of angiogenesis was consistent and correlated well with the tempo of healing of the acute wounds. These preliminary data demonstrate the more rapid acute wound healing properties of the combination formulation of thymosin β4 and silver sulfadiazine as compared to these agents alone. This novel agent could prove an effective treatment modality for debilitating chronic wounds and decubitus ulcers.
Department of Surgery, Columbia University, New York, New York 10032, USA. suman.paritosh@gmail.com
Paritosh
P
Suman
Harikrishnan
H
Ramachandran
Sossy
S
Sahakian
Kamraan Z
KZ
Gill
Basil A J
BA
Horst
Shanta M
SM
Modak
Mark A
MA
Hardy
D000818:Animals; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D003668:Pressure Ulcer / Q000188:drug therapy / Q000378:metabolism; D051381:Rats; D012837:Silver Sulfadiazine / Q000627:therapeutic use*; D013947:Thymosin / Q000627:therapeutic use*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*; D000099201:Thymosin beta(4)
D000890:Anti-Infective Agents; D012837:Silver Sulfadiazine; D013947:Thymosin; D042461:Vascular Endothelial Growth Factor A; D000099201:Thymosin beta(4)