[Development and validation of a GC-MS method for determination of nicotine in tobacco].
DETERMINATION FROM TOBA gas chromatographic-mass spectrometric method (GC/MS) for determination of nicotine from tobacco products was developed and validated.


METHODS
It was used an Agilent Technologies 7890A Chromatograph equipped with a DB 5 MS column (30 m x 0.25 mm, 0.25 microm) and Agilent Technologies 5975C inert MSD detector. The temperature program began from 100 degrees C and increase with 10 degrees C/min to 190 degrees C than with 20 degrees C/min to 280 degrees C, constant for 5 min, with He as carrier gas (1 ml/min) and MS detection. The temperature of the source was 230 degrees C and the temperature of the quadrupole was 150 degrees C. The parameters of validation were studied according to the international requirements.


RESULTS
Finally, the method established has following parameters: is linear in the 1.01-201.8 microg/ml range, has the detection limit of 3.6 microg/ml, the quantification limit of 10.8 microg/ml, is precise (RSD < 5%, n = 9) and accurate (mean recovery is 100.28% in 99.08-100.96 microg/ml range).


CONCLUSIONS
The method can be used to determine nicotine from tobacco products, with good results.
Universităţii de Medicină şi Farmacie "Grigore T. Popa"--Iaşi.
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D000465:Algorithms; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009538:Nicotine / Q000032:analysis*; D015203:Reproducibility of Results; D013696:Temperature; D014026:Nicotiana / Q000737:chemistry*
D009538:Nicotine