Evaluation of tissue response and genotoxicity of poly(L/D-lactic acid) disks implanted in rat calvarium.
The aim of the present study was to evaluate local and cytotoxicity systemic tissue reaction in the skull of rats using the implantation of disks of poly (lactic L/D-acid) and evaluate its genotoxicity. 25 males Wistar rats were used, 20 animals underwent surgical procedures and had the discs implanted in the parietal bone, and 5 animals received postoperative medication in the same way, serving as a control group for genotoxicity. The results were subjected to statistical evaluation by analysis of variance (ANOVA). In histological evaluation, between periods of 90 and 120 days in the control group, a new formation at the edges of the defect was noticed. In the experimental group, there was new bone formation at the edges of the defect, migrating below the site occupied by the disk, an absence of inflammatory infiltrate. Regarding the evaluation of genotoxicity, a significant reduction in the frequency of polychromatic erythrocytes in relation to negative control or significant increase in the polychromatic erythrocytes with micronuclei was not detected. So, the material used in this study is biocompatible and well tolerated by the tissues studied, and found to be negative for chromosomal mutagenicity.
Department of Oral and Maxillofacial Surgery, Pontificial Catholic University of Rio Grande do Sul, PUC/RS, Porto Alegre, Rio Grande do Sul, Brazil. olhj@hotmail.com
J G
JG
Göelzer
R L
RL
Avelar
A N
AN
Dolzan
O E
OE
Becker
O L
OL
Haas
L Y S
LY
de Oliveira
R B
RB
de Oliveira
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000633:toxicity; D004343:Drug Implants / Q000008:administration & dosage / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects / Q000473:pathology; D019344:Lactic Acid / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D008422:Materials Testing; D015162:Micronucleus Tests; D009153:Mutagens / Q000008:administration & dosage / Q000633:toxicity*; D011091:Polyesters; D011108:Polymers / Q000008:administration & dosage / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000187:drug effects* / Q000473:pathology
D001672:Biocompatible Materials; D004343:Drug Implants; D009153:Mutagens; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)