In vitro toxicity and antidiabetic activity of a newly developed polyherbal formulation (MAC-ST/001) in streptozotocin-induced diabetic Wistar rats.
The present study was designed to investigate the hypoglycemic effect of an aqueous extract of MAC-ST/001 (a new polyherbal formulation) which was given once daily to rats at different doses. The animals were divided into diabetic and nondiabetic control groups. The duration of each experiment lasted from 1 week to 1 month, and the results were compared with that of the standard hypoglycemic drug glibenclamide (10 mg/kg), which was given once daily. In this study, biochemical and histopathological parameters were studied in streptozotacin (STZ) (single intraperitoneal injection of 55 mg/kg)-induced diabetic rats. The diabetic rats showed a significant (p < 0.05 and p < 0.01) decrease in their body weight and serum amylase with marked elevation in blood glucose, serum cholesterol, blood urea nitrogen, creatinine, alkaline phosphatase, and serum transaminases (AST and ALT) after 1 week till the 28th day of diabetes. Cytotoxicity of MAC-ST/001 formulation was also studied on C2C12, 3T3-L1, and HepG2 cells through MTT assay. Histological examination of the liver and pancreas of normal control, diabetic control, and drug-treated rats revealed significant results. Finally, it was concluded that administration of this MAC-ST/001 extract reversed most blood and tissue changes caused by STZ-induced diabetes in rats.
Drug Standardization Lab, Department of Ilmul-Advia, F/O Medicine, Jamia Hamdard, New Delhi, 110062, India. deepaknano@gmail.com
Deepak
D
Yadav
Anis Ahmad
AA
Chaudhary
Veena
V
Garg
Mohammad Faiyaz
MF
Anwar
Md Mahfooz-ur
MM
Rahman
Sayed Sakir
SS
Jamil
Haider Ali
HA
Khan
Mohd
M
Asif
D041721:3T3-L1 Cells; D000818:Animals; D031601:Azadirachta / Q000737:chemistry; D001786:Blood Glucose; D001835:Body Weight / Q000187:drug effects; D029911:Caesalpinia / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy*; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D029777:Momordica charantia / Q000737:chemistry; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry; D013311:Streptozocin; D027842:Syzygium / Q000737:chemistry; D029910:Trigonella / Q000737:chemistry
D001786:Blood Glucose; D007004:Hypoglycemic Agents; C585240:MAC-ST-001; D010936:Plant Extracts; D013311:Streptozocin