Transcriptome analysis of quantitative resistance-specific response upon Ralstonia solanacearum infection in tomato.
Bacterial wilt, caused by the soil-borne bacterium Ralstonia solanacearum, is a lethal disease of tomato, but the molecular mechanisms of the host resistance responses to R. solanacearum remain unclear. In this study, we report the first work describing the transcriptome of cultivar resistance and susceptible tomato cultivar after inoculation with R. solanacearum. To elucidate the characteristics of resistance early in the interaction, we analyzed microarrays for resistant cultivar LS-89 and susceptible cultivar Ponderosa 1 day after stem inoculation. No change in gene expression was detected for Ponderosa, but expression levels of over 140 genes, including pathogenesis-related, hormone signaling and lignin biosynthesis genes, increased in LS-89. Expression of β-1,3-glucanase genes increased substantially. In an immunohistochemical study, glucanase in LS-89 accumulated in the xylem and pith tissues surrounding xylem vessels filled with R. solanacearum. The expression of these genes also increased in four other resistant cultivars, but changed little in four susceptible cultivars in response to R. solanacearum, suggesting that similar reactions occur in other cultivars. These gene expression profiles will serve as fundamental information to elucidate the molecular mechanisms in the resistance response to R. solanacearum in tomato.
Plant Protection Division, National Agricultural Research Center, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan.
Takeaki
T
Ishihara
Ichiro
I
Mitsuhara
Hideki
H
Takahashi
Kazuhiro
K
Nakaho
D060467:Disease Resistance / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D054884:Host-Pathogen Interactions; D008031:Lignin / Q000378:metabolism; D018551:Solanum lycopersicum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D043368:Ralstonia solanacearum / Q000276:immunology / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D008031:Lignin; D006026:Glycoside Hydrolases