Cloning  and expression of ethylene receptor ERS1 at various developmental and ripening  stages of mango fruit.
Ethylene induces characteristic ripening reactions in climacteric fruits through its binding to histidine-kinase (HK) receptors, activating the expression of ripening genes. Ethylene receptors have been found in Arabidopsis thaliana (Brassicaceae) and some fruits; number and expression patterns differ among species. In mango, only ethylene receptor ETR1 was known. We cloned ERS1 cDNA from mango, and evaluated the expression of Mi-ERS1 and Mi-ETR1 by qPCR in developmental and ripening stages of this fruit. The Mi-ERS1 coding sequence is 1890 bp long and encodes 629 amino acids, similar to ERS1 from other fruits. Also, the amino acid sequence of ERS1 C-terminal HK domain shows the cognate fold after molecular modeling. Mi-ERS1 expression levels increased as mangoes ripened, showing the highest levels at the climacteric stage, while Mi-ETR1 levels did not change during development and ripening. We conclude that the patterns of expression of Mi-ERS1 and Mi-ETR1 differ in mango fruit.
Centro de Investigación en Alimentación y Desarrollo, Hermosillo, Sonora, México.
C A
CA
Contreras-Vergara
N A
NA
Stephens-Camacho
G
G
Yepiz-Plascencia
G A
GA
González-Aguilar
A A
AA
Arvizu-Flores
E
E
Sanchez-Sanchez
M A
MA
Islas-Osuna
D000595:Amino Acid Sequence; D001483:Base Sequence; D002245:Carbon Dioxide / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005638:Fruit / Q000235:genetics / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D031022:Mangifera / Q000235:genetics* / Q000254:growth & development*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D018076:DNA, Complementary; D010940:Plant Proteins; D011956:Receptors, Cell Surface; C083750:ethylene receptors, plant; D002245:Carbon Dioxide