Complementary function of mitogen-activated protein kinase Hog1 from Trichosporonoides megachiliensis in Saccharomyces cerevisiae under hyper-osmotic stress.
A (TmHog1) gene encoding a mitogen-activated protein kinase (MAPK) homologous to Saccharomyces cerevisiae Hog1 (ScHog1) involved in hyper-osmotic stress signaling was isolated from Trichosporonoides megachiliensis SN-124A, an erythritol-producing yeast. Although TmHog1, like other Hog1 homologs, encoded a kinase catalytic domain containing TGY motif, it was 50-60 amino acid residues shorter than the ScHog1. A TmHog1 transgene rescued the osmotic sensitivity and glycerol production defect of S. cerevisiae hog1Δ, a highly osmo-sensitive strain that does not produce glycerol, a compatible solute, during osmotic stress. Functional analyses of chimeric Hog1 proteins constructed from ScHog1 and TmHog1 sequences indicated that the C-terminal region of TmHog1 is more effective for glycerol biosynthesis than ScHog1 under osmotic stress.
College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa 252-0880, Japan.
Junjiro
J
Yoshida
Yosuke
Y
Kobayashi
Yosuke
Y
Tanaka
Yoshiyuki
Y
Koyama
Jun
J
Ogihara
Jun
J
Kato
Jun
J
Shima
Takafumi
T
Kasumi
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics; D001483:Base Sequence; D049109:Cell Proliferation; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005816:Genetic Complementation Test; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009997:Osmotic Pressure / Q000187:drug effects; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics
D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D020928:Mitogen-Activated Protein Kinases; D005990:Glycerol