Avidin functionalized maghemite nanoparticles and their application for recombinant human biotinyl-SERCA purification.
We report on the surface characterization, functionalization, and application of stable water suspensions of novel surface active maghemite nanoparticles (SAMNs), characterized by a diameter of 11 ± 2 nm and possessing peculiar colloidal properties and surface interactions. These features permitted the acquisition of titration curves and aqueous UV-vis spectra and suggested a role played by surface under-coordinated iron atoms. This new class of nanoparticles was obtained through an easy, inexpensive, one-step, green procedure and functionalized with ligands of high biotechnological interest, such as biotin and avidin, by simple incubation in aqueous solution. Bound avidin was determined by measuring the disappearance of free avidin absorbance at 280 nm, as a function of increasing nanoparticle concentration, showing the presence of 10 ± 3 avidin molecules per nanoparticle. The biological activity of the SAMN@avidin complex was evaluated and the number of available biotin binding sites was determined, using biotinyl-fluorescein as a probe, showing that each bound avidin molecule is able to bind 2.8 ± 0.8 biotin molecules, confirming the maintenance of biological activity and excellent binding capacity of the SAMN@avidin complex. Furthermore a Langmuir isotherm model was used to describe the biomolecule specific monolayer adsorption onto the particle surface, and in the case of avidin, the maximum adsorption capacity was 100 ± 27 μg avidin/mg SAMN, whereas the binding constant is 45.18 μL μg(-1). The SAMN@avidin complex was characterized by UV-vis spectroscopy, quartz crystal microbalance, FTIR spectroscopy, and transmission electron microscopy. Finally, SAMN@avidin was applied for the large scale purification of recombinant biotinylated human sarco/endoplasmic reticulum Ca(2+)-ATPase (hSERCA-2a), expressed by Saccharomyces cerevisiae. The protein was magnetically purified, and about 500 μg of a 70% pure hSERCA-2a were recovered from 4 L of yeast culture, with a purification yield of 64%.
Department of Comparative Biomedicine and Food Science, University of Padua, Italy.
Massimiliano
M
Magro
Adele
A
Faralli
Davide
D
Baratella
Ilenia
I
Bertipaglia
Sara
S
Giannetti
Gabriella
G
Salviulo
Radek
R
Zboril
Fabio
F
Vianello
D001360:Avidin / Q000737:chemistry* / Q000378:metabolism*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism*; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013499:Surface Properties
D005290:Ferric Compounds; D011994:Recombinant Proteins; D001360:Avidin; C000499:ferric oxide; D001710:Biotin; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases