Biocontrol of anthracnose in pepper using chitinase, beta-1,3 glucanase, and 2-furancarboxaldehyde produced by Streptomyces cavourensis SY224.
A strain of Streptomyces cavourensis subsp. cavourensis (coded as SY224) antagonistic to Colletotrichum gloeosporioides infecting pepper plants was isolated. SY224 produced lytic enzymes such as chitinase, beta-1,3-glucanase, lipase, and protease in respective assays. To examine for antifungal activity, the treatments amended with the nonsterilized supernatant resulted in the highest growth inhibition rate of about 92.9% and 87.4% at concentrations of 30&percnt; and 10&percnt;, respectively. However, the sterilized treatments (autoclaved or chloroform treated) gave a lowered but significant inhibitory effect of about 63.4% and 62.6% for the 10% supernatant concentration, and 75.2% and 74.8% for the of 30% supernatant concentration in the PDA agar medium, respectively, indicative of the role of a nonprotein, heat stable compound on the overall effect. This antifungal compound, which inhibited spore germination and altered hyphal morphology, was extracted by EtOAc and purified by ODS, silica gel, Sephadex LH-20 column, and HPLC, where an active fraction was confirmed to be 2-furancarboxaldehyde by GS-CI MS techniques. These results suggested that SY224 had a high potential in the biocontrol of anthracnose in pepper, mainly due to a combined effect of lytic enzymes and a non-protein, heatstable antifungal compound, 2-furancarboxaldehyde.
Department of Agricultural Chemistry, Chonnam National University, Gwangju 500-757, Korea.
So Youn
SY
Lee
Hamisi
H
Tindwa
Yong Seong
YS
Lee
Kyaw Wai
KW
Naing
Seong Hyun
SH
Hong
Yi
Y
Nam
Kil Yong
KY
Kim
D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D061046:Biological Control Agents; D002212:Capsicum / Q000382:microbiology*; D002686:Chitin / Q000737:chemistry; D002688:Chitinases / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002725:Chloroform / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D020231:Colletotrichum / Q000187:drug effects / Q000254:growth & development / Q000472:pathogenicity; D004789:Enzyme Activation; D005663:Furans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D043326:Glucan 1,3-beta-Glucosidase / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D008049:Lipase / Q000737:chemistry / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D058428:Silica Gel / Q000737:chemistry; D013172:Spores, Fungal / Q000187:drug effects / Q000254:growth & development; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology* / Q000302:isolation & purification
D000935:Antifungal Agents; D001426:Bacterial Proteins; D061046:Biological Control Agents; D005663:Furans; D002686:Chitin; D058428:Silica Gel; D002725:Chloroform; D008049:Lipase; D002688:Chitinases; D043326:Glucan 1,3-beta-Glucosidase