Enhanced wound healing effect of canine adipose-derived mesenchymal stem cells with low-level laser therapy in athymic mice.
BACKGROUND
Adipose-derived mesenchymal stem cells (ASCs) are attractive cell source for skin tissue engineering. However, one obstacle to this approach is that the transplanted ASC population can decline rapidly in the recipient tissue.


OBJECTIVE
The aim of this study was to investigate the effects of low-level laser therapy (LLLT) on transplanted canine ASCs in a skin wound animal model.


METHODS
LLLT, ASC transplantation (ASCs) and ASC transplantation with LLLT (ASCs+LLLT) were applied to the wound bed in athymic mice. Wound healing was assessed by gross evaluation and by hematoxylin and eosin staining. The survival, differentiation and secretion of vascular endothelial growth factor and basic fibroblast growth factor of the ASCs were evaluated by immunohistochemistry and Western blotting.


RESULTS
The ASCs and ASCs+LLLT groups stimulated wound closure and histological skin regeneration. The ASCs+LLLT group enhanced the wound healing, including neovascularization and regeneration of skin appendages, compared with the ASCs group. The ASCs contributed skin regeneration via differentiation and secretion of growth factors. In the ASCs+LLLT group, the survival of ASCs was increased by the decreased apoptosis of ASCs in the wound bed. The secretion of growth factors was stimulated in the ASCs+LLLT group compared with the ASCs group.


CONCLUSIONS
These data suggest that LLLT is an effective biostimulator of ASCs in wound healing that enhances the survival of ASCs and stimulates the secretion of growth factors in the wound bed.
Department of Veterinary Surgery, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Hyoju
H
Kim
Kyuseok
K
Choi
Oh-Kyeong
OK
Kweon
Wan Hee
WH
Kim
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002454:Cell Differentiation; D002470:Cell Survival / Q000528:radiation effects; D004285:Dogs; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D007633:Keratins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D028022:Low-Level Light Therapy; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology / Q000528:radiation effects*; D051379:Mice; D008819:Mice, Nude; D012867:Skin / Q000293:injuries / Q000473:pathology / Q000528:radiation effects; D023822:Tissue Engineering; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects*
D019394:Ki-67 Antigen; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D016222:Fibroblast Growth Factor 2; D007633:Keratins; C505409:Casp3 protein, mouse; D053148:Caspase 3