Foxp3 expression in bovine CD8+ T cells is associated with the intensity of CD25 expression.
The aim of the undertaken research was to determine whether Foxp3(+)CD25(+)CD8(+) cells exist in cattle and whether Foxp3 expression in CD8(+) T cells is correlated with the intensity of CD25 expression. It has been found that 0.66 and 2.36% of CD8(+) cells on average showed high and low expression of the CD25 molecule, respectively. On average, 11.61% of CD25(high)CD8(+) cells expressed Foxp3, while the mean percentage of Foxp3(+) cells within CD25(low)CD8(+) cells was 3.66%. The absolute count of Foxp3(+)CD25(high)CD8(+) cells was not significantly different from the absolute count of Foxp3(+)CD25(low)CD8(+) cells. The obtained results indicate that CD8(+) cells with the regulatory phenotype, i.e. Foxp3(+)CD25(high)CD8(+) and Foxp3(+)CD25(low)CD8(+) cells, occur naturally in bovine peripheral blood, although both of these subpopulations are relatively small. There is a positive correlation between the intensity of CD25 expression and the expression of the transcription Foxp3 factor in bovine CD8(+) cells.
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland. tomasz.maslanka@uwm.edu.pl
Tomasz
T
Maślanka
Jerzy Jan
JJ
Jaroszewski
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D002417:Cattle; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000235:genetics / Q000378:metabolism*
D004268:DNA-Binding Proteins; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit