PPARA, RXRA, NR1I2 and NR1I3 gene polymorphisms and lipid and lipoprotein levels in a Southern Brazilian population.
Cardiovascular disease is the main cause of death worldwide, and dyslipidemia is an important multifactorial risk factor. Considering the involvement of nuclear receptors in metabolic pathways, and that some of the receptors act in lipid metabolism and homeostasis, the aim of the present study was to investigate the influence of genetic variations in RXRA, PPARA, NR1I2, and NR1I3 on lipid and lipoprotein levels. Five polymorphisms in the aforementioned genes were genotyped in 622 Brazilians of European descent by PCR-RFLP or TaqMan genotyping assays. In general, carriers of the A insertion of RXRA rs11381416 polymorphism showed higher levels of triglyceride (TG; 1.80 ± 1.20 vs. 1.52 ± 1.20 mmol/L; P = 0.020). Moreover, sexual dimorphic association was found (gender*NR1I3 rs2501873 genotype interaction P < 0.001), males with NR1I3 rs2501873 G/G genotype had lower TG levels (ANCOVA, P = 0.009). Our results suggest that polymorphisms in the RXRA and NR1I3 genes influence lipid profile in a Southern Brazilian population. However, these general and gender association require confirmation in subsequent studies.
Laboratório de Biologia Molecular, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Brazil.
Luciana O
LO
Lima
Silvana
S
Almeida
Mara H
MH
Hutz
Marilu
M
Fiegenbaum
D000328:Adult; D054458:Amplified Fragment Length Polymorphism Analysis; D001938:Brazil; D000090702:Constitutive Androstane Receptor; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D015228:Hypertriglyceridemia / Q000235:genetics; D015810:Linkage Disequilibrium; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008875:Middle Aged; D047493:PPAR alpha / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D000077297:Pregnane X Receptor; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D011987:Receptors, Steroid / Q000235:genetics*; D047490:Retinoid X Receptor alpha / Q000235:genetics*; D012306:Risk; D017422:Sequence Analysis, DNA; D012727:Sex Characteristics; D014280:Triglycerides / Q000097:blood*
D000090702:Constitutive Androstane Receptor; D008074:Lipoproteins; C000625852:NR1I2 protein, human; C000716327:NR1I3 protein, human; D047493:PPAR alpha; D000077297:Pregnane X Receptor; D018160:Receptors, Cytoplasmic and Nuclear; D011987:Receptors, Steroid; D047490:Retinoid X Receptor alpha; D014280:Triglycerides