Regional Biomechanical properties of human sclera after cross-linking by riboflavin/ultraviolet A.
OBJECTIVE
To evaluate the biomechanical difference of human scleral collagen cross-linking (CXL) by comparing different riboflavin-instilling methods and different cross-linked regions (equatorial and posterior sclera).


METHODS
Fifteen donor human eyes were randomly divided into five groups. One group, in which CXL was not applied, was designated as the control group. In the remaining four groups, 0.1% riboflavin solution was instilled on the scleral surface for 5, 10, 20, or 30 minutes, respectively, followed by 30 minutes of ultraviolet A irradiation. The equatorial and posterior scleral strips in each eye were dissected. Stress-strain measurements of all scleral strips were performed by a biomaterial tester. Young modulus was calculated at 8% strain. Data of the stress and Young modulus in different regions and groups were compared using one-way analysis of variance.


RESULTS
Under a 1 mm/minute stretching, the sclera exhibited an exponential stress-strain behavior. The stress and modulus of equatorial and posterior sclera after CXL gradually increased with riboflavin instillation before surgery. No statistical difference was noted in the modulus between 20 and 30 minutes riboflavin infiltration after CXL (P>.05). At the same strain levels, equatorial sclera with and without CXL exhibited higher stress and Young modulus than that of posterior sclera.


CONCLUSIONS
Equatorial and posterior human sclera may be enhanced by CXL with riboflavin/ultraviolet A irradiation. Equatorial scleral CXL may be a good choice for the treatment of progressive myopia. Because of its safety and efficacy, 20 minutes of riboflavin infiltration before CXL is recommended.
Beijing Tongren Eye Center, Beijing Tongren Hospital, Beijing Ophthalmology & Visual Sciences Key Lab, Beijing, China.
Mengmeng
M
Wang
Fengju
F
Zhang
Xiuqing
X
Qian
Xu
X
Zhao
D000328:Adult; D000368:Aged; D001696:Biomechanical Phenomena; D003094:Collagen / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D055119:Elastic Modulus; D004548:Elasticity / Q000502:physiology*; D006801:Humans; D008875:Middle Aged; D017319:Photosensitizing Agents / Q000494:pharmacology*; D010865:Pilot Projects; D012256:Riboflavin / Q000494:pharmacology*; D012590:Sclera / Q000187:drug effects / Q000502:physiology*; D014019:Tissue Donors; D014466:Ultraviolet Rays; D055815:Young Adult
D003432:Cross-Linking Reagents; D017319:Photosensitizing Agents; D003094:Collagen; D012256:Riboflavin