Interaction of different extracts of Primula heterochroma Stapf. with red blood cell membrane lipids and proteins: antioxidant and antihemolytic effects.
In recent years, considerable attention has been paid to plants as potent natural drugs for their ameliorative roles against free-radical-mediated oxidative stress. Therefore, their interactions with cell membrane lipids and proteins, which generally serve as primary targets of lipid peroxidation, are of much interest. In the current investigation, in vitro and ex vivo studies are performed in order to estimate possible effects of different extracts of Primula heterochroma Stapf. on red blood cell membranes of rat erythrocytes using colorimetric methods. The results indicate that binding of the extracts to lipids and proteins of red blood cell membranes both significantly inhibits lipid peroxidation, and also increases red blood cell integrity against hemolysis. Moreover, a polyphenol extract, in particular, demonstrates notable antihemolytic activity in hydrogen peroxide-induced hemolysis model (IC(50) = 199.49 ± 9.1 μg ml(-1)).
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Nabavi208@gmail.com
Seyed Mohammad
SM
Nabavi
Seyed Fazel
SF
Nabavi
William N
WN
Setzer
Heshmatollah
H
Alinezhad
Mahboobeh
M
Zare
Alireza
A
Naqinezhad
D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage / Q000302:isolation & purification; D003124:Colorimetry; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000187:drug effects*; D005419:Flavonoids / Q000008:administration & dosage / Q000302:isolation & purification; D006461:Hemolysis / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008563:Membrane Lipids / Q000097:blood*; D008565:Membrane Proteins / Q000097:blood*; D010936:Plant Extracts / Q000378:metabolism* / Q000494:pharmacology*; D059808:Polyphenols / Q000008:administration & dosage / Q000302:isolation & purification; D011134:Polysaccharides / Q000008:administration & dosage / Q000302:isolation & purification; D031825:Primula / Q000737:chemistry*; D051381:Rats
D000872:Anthocyanins; D005419:Flavonoids; D008563:Membrane Lipids; D008565:Membrane Proteins; D010936:Plant Extracts; D059808:Polyphenols; D011134:Polysaccharides