In vitro electroretinogram for the study of the functionality of differentiated retinal pigment epithelium cells.
The purpose of this study is to develop and test a method to reveal if the retinal pigment epithelium (RPE) cells differentiated from human embryonic stem cells (hESC) support the functions of photoreceptors. hESC-derived RPE (hESC-RPE) cells offer a potent cell source for cell replacement therapy that may be used to prevent certain eye diseases. Methods to assure the functionality of the RPE cells are well warranted. Electroretinograms (ERG) measure the electrophysiological response of the retina to light stimuli. A setup was developed that enables the measurement of ERG in vitro from mice retinas cultured together with hESC-RPE cells. The co-culture of RPE and retinas seems to be a viable tool to assess the functionality of RPE in vitro. However, owing to limited sample size results were somewhat mixed, and thus it was not possible to prove that hESC-RPE cells enhance the ERG response of a mouse retina in vitro. The long-term culturing of the retinas needs to be refined to acquire more conclusive evidence of the supporting role of the RPE and to explore the full potential of the co-culture and ERG methods in assessing RPE functionality.
Department of Biomedical Engineering, Tampere University of Technology and BioMediTech, Tampere, Finland. niina.onnela@tut.fi
Niina
N
Onnela
Leena
L
Lehtonen
Mikko
M
Koski
Jari
J
Hyttinen
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D018920:Coculture Techniques; D004566:Electrodes; D004596:Electroretinography / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D055213:Retinal Pigment Epithelium / Q000166:cytology*