The experimental renal cell carcinoma model in the chick embryo.
The clear cell subtype of renal carcinoma (CCRCC) is highly vascularized and despite a slow progression rate, it is potentially a highly aggressive tumor. Although a doubling of median progression-free survival in CCRCC patients treated by targeted therapies has been observed, the fact that tumors escape after anti-VEGF treatment suggests alternative pathways. The chick chorioallantoic membrane (CAM) is a well-established model, which allows in vivo studies of tumor angiogenesis and the testing of anti-angiogenic molecules. However, only a few data exist on CCRCC grafted onto CAM. We aimed to validate herein the CAM as a suitable model for studying the development of CCRCC and the interactions with the surrounding stroma. Our study uses both CCRCC cell lines and fresh tumor samples after surgical resection. We demonstrate that in both cases CCRCC can be grafted onto the CAM, to survive and to induce an angiogenic process. We further provide insights into the transcriptional regulation of the model by performing a differential analysis of tumor-derived and stroma-derived transcripts.
INSERM U, Talence, France.
Patricia
P
Fergelot
Jean-Christophe
JC
Bernhard
Fabienne
F
Soulet
Witold W
WW
Kilarski
Céline
C
Léon
Nathalie
N
Courtois
Colette
C
Deminière
John M J
JM
Herbert
Philipp
P
Antczak
Francesco
F
Falciani
Nathalie
N
Rioux-Leclercq
Jean-Jacques
JJ
Patard
Jean-Marie
JM
Ferrière
Alain
A
Ravaud
Martin
M
Hagedorn
Andreas
A
Bikfalvi
D000818:Animals; D002292:Carcinoma, Renal Cell / Q000098:blood supply / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000473:pathology; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000098:blood supply / Q000235:genetics / Q000473:pathology*; D018613:Microscopy, Confocal; D055806:Microvessels / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology; D010641:Phenotype; D015854:Up-Regulation / Q000235:genetics; D023041:Xenograft Model Antitumor Assays