The chemical identification and analysis of Aspergillus nidulans secondary metabolites.
Filamentous fungi have long been recognized to be a rich source of secondary metabolites with potential medicinal applications. The recent genomic sequencing of several Aspergillus species has revealed that many secondary metabolite gene clusters are apparently silent under standard laboratory conditions. Several successful approaches have been utilized to upregulate these genes and unearth the corresponding natural products. A straightforward, reliable method to purify and characterize new metabolites therefore should be useful. Details are provided herein on the cultivation of Aspergillus nidulans and the LC/MS analysis of the metabolic profile. Following is an explanation of silica gel chromatography, HPLC, and preparative TLC. Finally, the NMR characterization of previously unknown A. nidulans metabolites is detailed.
Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, CA, USA.
James F
JF
Sanchez
Clay C C
CC
Wang
D001233:Aspergillus nidulans / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D046508:Culture Techniques; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D061065:Polyketides / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry; D013172:Spores, Fungal / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism
D061065:Polyketides; D012822:Silicon Dioxide