Shrimp (Litopenaeus vannamei) trypsinogen production in Pichia pastoris bioreactor cultures.
Recently, we engineered a Pichia pastoris Mut(+) strain to produce and secrete recombinant Litopenaeus vannamei trypsinogen. Despite the observed toxicity of the recombinant shrimp trypsinogen to the P. pastoris cell host, when high density cell cultures in shake flasks with alanine in the induction medium were used recombinant shrimp trypsinogen could be produced. To further improve the product yield, in this work, we evaluated L. vannamei trypsinogen production in P. pastoris using a bioreactor and two recombinant P. pastoris strains with different methanol utilization (Mut) phenotypes. The effect of pH and temperature during the induction step on the trypsinogen production was also evaluated. The results indicate that temperature, pH, and Mut phenotypes influence the production of the recombinant protein, with almost no observed effect on cell growth. All cultures with the Mut(+) strain had significant operational difficulties, such as in lowering the induction temperature, maintaining dissolved oxygen (DO) above 20%, and maintaining the methanol concentration at a constant value, and showed a decrease in metabolic activity due to trypsinogen toxicity to the cell host. In the culture with the Mut(s) strain, however, the temperature, methanol concentration, and DO could be more easily controlled, the temperature could be easily decreased, and the trypsinogen caused the lowest toxicity to the host cells. After 96 h of Mut(s) strain induction (pH 6 and 25°C), about 250 mg/L recombinant trypsinogen was detected in the culture medium.
Universidad Autónoma de Nuevo León, UANL, Facultad de Ciencias Biológicas, Instituto de Biotecnología, San Nicolás de los Garza, Nuevo León, México.
José M
JM
Viader-Salvadó
José A
JA
Fuentes-Garibay
Mauricio
M
Castillo-Galván
María M
MM
Iracheta-Cárdenas
Luis J
LJ
Galán-Wong
Martha
M
Guerrero-Olazarán
D000818:Animals; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D000432:Methanol / Q000737:chemistry; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism*; D010843:Pichia / Q000166:cytology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014362:Trypsinogen / Q000096:biosynthesis*
D011994:Recombinant Proteins; D014362:Trypsinogen; D000432:Methanol