Role for apyrases in polar auxin transport in Arabidopsis.
Recent evidence indicates that extracellular nucleotides regulate plant growth. Exogenous ATP has been shown to block auxin transport and gravitropic growth in primary roots of Arabidopsis (Arabidopsis thaliana). Cells limit the concentration of extracellular ATP in part through the activity of ectoapyrases (ectonucleoside triphosphate diphosphohydrolases), and two nearly identical Arabidopsis apyrases, APY1 and APY2, appear to share this function. These findings, plus the fact that suppression of APY1 and APY2 blocks growth in Arabidopsis, suggested that the expression of these apyrases could influence auxin transport. This report tests that hypothesis. The polar movement of [(3)H]indole-3-acetic acid in both hypocotyl sections and primary roots of Arabidopsis seedlings was measured. In both tissues, polar auxin transport was significantly reduced in apy2 null mutants when they were induced by estradiol to suppress the expression of APY1 by RNA interference. In the hypocotyl assays, the basal halves of APY-suppressed hypocotyls contained considerably lower free indole-3-acetic acid levels when compared with wild-type plants, and disrupted auxin transport in the APY-suppressed roots was reflected by their significant morphological abnormalities. When a green fluorescent protein fluorescence signal encoded by a DR5:green fluorescent protein construct was measured in primary roots whose apyrase expression was suppressed either genetically or chemically, the roots showed no signal asymmetry following gravistimulation, and both their growth and gravitropic curvature were inhibited. Chemicals that suppress apyrase activity also inhibit gravitropic curvature and, to a lesser extent, growth. Taken together, these results indicate that a critical step connecting apyrase suppression to growth suppression is the inhibition of polar auxin transport.
Department of Horticultural Science and Microbial and Plant Genomics Institute, University of Minnesota, St. Paul, Minnesota 55108, USA.
Xing
X
Liu
Jian
J
Wu
Greg
G
Clark
Stacey
S
Lundy
Minhui
M
Lim
David
D
Arnold
Jing
J
Chan
Wenqiang
W
Tang
Gloria K
GK
Muday
Gary
G
Gardner
Stanley J
SJ
Roux
D001081:Apyrase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D060146:Ecotype; D004958:Estradiol / Q000494:pharmacology; D005453:Fluorescence; D006112:Gravitation; D018522:Gravitropism / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018546:Hypocotyl / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D007210:Indoleacetic Acids / Q000378:metabolism*; D008938:Mitosis / Q000187:drug effects; D009711:Nucleotides / Q000378:metabolism / Q000494:pharmacology; D018517:Plant Roots / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D034622:RNA Interference / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013489:Suppression, Genetic / Q000187:drug effects
D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D009711:Nucleotides; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D004958:Estradiol; C522733:APY1 protein, Arabidopsis; C522734:APY2 protein, Arabidopsis; D001081:Apyrase