A gel-based visual immunoassay for non-instrumental detection of chloramphenicol in food samples.
A gel-based non-instrumental immuno-affinity assay was developed for the rapid screening of chloramphenicol (CAP) in food samples with the limit of detection (LOD) of 1 μg L(-1). The immuno-affinity test column (IATC) consisted of a test layer containing anti-CAP antibody coupled gel, and a control layer with anti-HRP antibody coupled gel. Based on the direct competitive immuno-reaction and the horseradish peroxidase enzymatic reaction, the test results could be evaluated visually. Basically, blue color development represented the negative results, while the absence of color development represented the positive results. In this study, CAP spiked samples of raw milk, pasteurized milk, UHT milk, skimmed milk powder, acacia honey, date honey, fish and shrimp were tested. Little or none sample pretreatment was required for this assay. The whole procedure was completed within 10min. In conclusion, the gel-based immuno-affinity test is a simple, rapid, and promising on-site screening method for CAP residues in food samples, with no instrumental requirement.
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China.
Meng
M
Yuan
Wei
W
Sheng
Yan
Y
Zhang
Junping
J
Wang
Yijin
Y
Yang
Shuguang
S
Zhang
Irina Yu
IY
Goryacheva
Shuo
S
Wang
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002701:Chloramphenicol / Q000032:analysis* / Q000276:immunology; D004867:Equipment Design; D005396:Fish Products / Q000032:analysis; D005506:Food Contamination / Q000032:analysis* / Q000191:economics; D005782:Gels / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry; D013997:Time Factors
D000900:Anti-Bacterial Agents; D005782:Gels; D002701:Chloramphenicol