Molecular cloning, tissue expression and SNP analysis in the goat nerve growth factor gene.
In this study, we cloned the full coding region of NGF gene from the caprine ovary. Result showed the caprine NGF cDNA (GenBank Accession No. JQ308184) contained a 726 bp open reading frame encoding a protein with 241 amino acid residues. Bioinformatic analysis indicated that caprine NGF amino acid sequence was 83-99 % identical to that of mouse, pig, dog, human and bovine. It was predicted that caprine NGF contained nine serine phosphorylation loci, four threonine phosphorylation loci and nine specific PKC phosphorylation loci. The NGF mRNA expression pattern showed that NGF gene was expressed highly in ovary. This work provided an important experimental basis for further research on the function of NGF in goat. A single nucleotide polymorphism (A705G) in the coding region of NGF gene was detected by PCR-RFLP and DNA sequencing in 630 goats of three breeds. The frequencies of G allele were 0.52-0.61, and frequencies of A allele were 0.48-0.39 for SN, GZ and BG breeds, respectively. The does with GG genotype had higher litter size than those with GA and AA genotypes (P < 0.05). Hence, the biochemical and physiological functions, together with the results obtained in our investigation, suggest that the NGF gene could serve as a genetic marker for litter size in goat breeding.
College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, People's Republic of China. anxiaopengdky@163.com
Xiaopeng
X
An
Long
L
Bai
Jinxing
J
Hou
Haibo
H
Zhao
Jiayin
J
Peng
Yunxuan
Y
Song
Jiangang
J
Wang
Binyun
B
Cao
D000595:Amino Acid Sequence; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D003001:Cloning, Molecular; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005819:Genetic Markers; D006041:Goats / Q000235:genetics*; D008098:Litter Size / Q000235:genetics; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D020932:Nerve Growth Factor / Q000235:genetics*; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014599:Uterus / Q000378:metabolism
D005819:Genetic Markers; D020932:Nerve Growth Factor