Simulation of arrhythmogenic effect of rogue RyRs in failing heart by using a coupled model.
Cardiac cells with heart failure are usually characterized by impairment of Ca(2+) handling with smaller SR Ca(2+) store and high risk of triggered activities. In this study, we developed a coupled model by integrating the spatiotemporal Ca(2+) reaction-diffusion system into the cellular electrophysiological model. With the coupled model, the subcellular Ca(2+) dynamics and global cellular electrophysiology could be simultaneously traced. The proposed coupled model was then applied to study the effects of rogue RyRs on Ca(2+) cycling and membrane potential in failing heart. The simulation results suggested that, in the presence of rogue RyRs, Ca(2+) dynamics is unstable and Ca(2+) waves are prone to be initiated spontaneously. These release events would elevate the membrane potential substantially which might induce delayed afterdepolarizations or triggered action potentials. Moreover, the variation of membrane potential depolarization is indicated to be dependent on the distribution density of rogue RyR channels. This study provides a new possible arrhythmogenic mechanism for heart failure from subcellular to cellular level.
Department of Biomedical Engineering, Wenzhou Medical College, Wenzhou 325035, China.
Luyao
L
Lu
Ling
L
Xia
Xiuwei
X
Zhu
D000200:Action Potentials; D001145:Arrhythmias, Cardiac / Q000378:metabolism; D002118:Calcium / Q000737:chemistry* / Q000378:metabolism*; D003198:Computer Simulation; D004058:Diffusion; D004594:Electrophysiology / Q000379:methods; D006333:Heart Failure / Q000378:metabolism*; D006352:Heart Ventricles / Q000166:cytology; D006801:Humans; D008564:Membrane Potentials; D015233:Models, Statistical; D008962:Models, Theoretical; D032342:Muscle Cells / Q000166:cytology; D019837:Ryanodine Receptor Calcium Release Channel / Q000378:metabolism*; D013997:Time Factors
D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium