Preparation of platelet-rich plasma from small volume of whole blood - a simplified approach.
OBJECTIVE
Platelet-rich plasma (PRP) is frequently used to enhance wound healing in numerous surgical applications. There are many methods of preparation of PRP. The present report describes a laboratory technique which is rapid, simple and inexpensive for preparing PRP from small volume of whole blood.


METHODS
Whole blood was drawn from 30 patients undergoing oral and maxillofacial surgery. PRP was prepared by the modified open system. Platelet counts for whole blood and PRP were determined using a cell counter.


RESULTS
The data from 30 patients aged 20 to 33 years, with a mean of 24.4 (± 3.3) and male : female ratio of 1:1.1, were analysed. The platelet count in PRP was 3,157,667 ± 984,380 while that in whole blood was 268,667 (± 64,678) platelets /cubic ml, reflecting an 11-fold increase in platelet concentration. No effect of gender or ageing on platelet count was observed.


CONCLUSIONS
This study demonstrates a simple, rapid and inexpensive technique that is useful in obtaining platelets concentrated approximately 11-fold.
Dental services department, Obafemi Awolowo University Teaching Hospitals complex, Ile-Ife, Osun state, Nigeria.
O K
OK
Ogundipe
V I
VI
Ugboko
F J
FJ
Owotade
B
B
Paul-Odo
A B
AB
Afariogun
D000328:Adult; D001793:Blood Preservation / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D019647:Oral Surgical Procedures / Q000009:adverse effects*; D019188:Organ Preservation Solutions / Q000494:pharmacology; D015539:Platelet Activation; D010976:Platelet Count; D053657:Platelet-Rich Plasma / Q000502:physiology*; D011183:Postoperative Complications / Q000097:blood / Q000503:physiopathology / Q000628:therapy*; D011300:Preoperative Care / Q000379:methods; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
D019188:Organ Preservation Solutions