Surface functionalized nanofibrillar cellulose (NFC) film as a platform for immunoassays and diagnostics.
We introduce a new method to modify films of nanofibrillated cellulose (NFC) to produce non-porous, water-resistant substrates for diagnostics. First, water resistant NFC films were prepared from mechanically disintegrated NFC hydrogel, and then their surfaces were carboxylated via TEMPO-mediated oxidation. Next, the topologically functionalized film was activated via EDS/NHS chemistry, and its reactivity verified with bovine serum albumin and antihuman IgG. The surface carboxylation, EDC/NHS activation and the protein attachment were confirmed using quartz crystal microbalance with dissipation, contact angle measurements, conductometric titrations, X-ray photoelectron spectroscopy and fluorescence microscopy. The surface morphology of the prepared films was investigated using confocal laser scanning microscopy and atomic force microscopy. Finally, we demonstrate that antihuman IgG can be immobilized on the activated NFC surface using commercial piezoelectric inkjet printing.
Department of Forest Products Technology, School of Chemical Technology, Aalto University, 00076 Espoo, Finland.
Hannes
H
Orelma
Ilari
I
Filpponen
Leena-Sisko
LS
Johansson
Monika
M
Osterberg
Orlando J
OJ
Rojas
Janne
J
Laine
D000327:Adsorption; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry* / Q000378:metabolism; D062028:Bioprinting / Q000379:methods; D002417:Cattle; D002482:Cellulose / Q000737:chemistry*; D003497:Cyclic N-Oxides; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D057139:Nanofibers / Q000737:chemistry*; D010084:Oxidation-Reduction; D058525:Quartz Crystal Microbalance Techniques; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry; D013499:Surface Properties
D055910:Antibodies, Immobilized; D003497:Cyclic N-Oxides; D007074:Immunoglobulin G; D013388:Succinimides; D012710:Serum Albumin, Bovine; D002482:Cellulose; C001426:N-hydroxysuccinimide; D005022:Ethyldimethylaminopropyl Carbodiimide; C003959:TEMPO