Effects of maternal and dietary selenium (Se-enriched yeast) on the expression of Sel P and apoER2 of germ cells of their offspring in goats.
In this experiment the effect of maternal dietary selenium on the expression of Sel P and apoER2 of goat offspring was studied. The experiment was conducted on 119 Taihang Black Goats randomly divided into 4 groups which were fed with a basal diet, supplemented with 0 (control), 0.5, 2 and 4 mg kg(-1) DM Se. Testis samples were collected from young male of each treatment group at the end of the study (30 d after weaning) for mRNA expression using real-time PCR and for protein expression by immunohistochemistry assay. A significant decrease was observed in mRNA expression of Sel P and apoER2 in the testis of the Se-deficient (Group 1) and the Se-excess (Group 4) compared with that in Groups 2 and 3. A similar trend of the protein expression of Sel P and apoER2 was also found. These data indicate that maternal and dietary selenium has an effect on the expression of Sel P and apoER2 in testis of their offspring. In addition, both groups were similar suggesting that the relationship between Sel P and apoER2, and apoER2 is a receptor of Sel P in the seminiferous epithelium to uptake the selenium.
College of Animal Science and Technology, Shanxi Agricultural University, Taigu, PR China.
Qian
Q
Wang
Lei
L
Shi
Youshe
Y
Ren
Wenbin
W
Yue
Chunxiang
C
Zhang
Fulin
F
Lei
D000818:Animals; D019587:Dietary Supplements; D005260:Female; D006041:Goats / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary; D026502:LDL-Receptor Related Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008297:Male; D018811:Maternal Exposure / Q000592:standards; D011247:Pregnancy; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012643:Selenium / Q000008:administration & dosage* / Q000378:metabolism; D051149:Selenoprotein P / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism*
D026502:LDL-Receptor Related Proteins; D051149:Selenoprotein P; C098773:low density lipoprotein receptor-related protein 8; D012313:RNA; D012643:Selenium