Silymarin attenuates benzo(a)pyrene induced toxicity by mitigating ROS production, DNA damage and calcium mediated apoptosis in peripheral blood mononuclear cells (PBMC).
Benzo(a)pyrene (B(a)P), which is the most studied member of PAH family is released into the environment (air, water and soil) from natural and man-made sources including industrial and automobile exhaust fumes. Since B(a)P is an omnipresent environmental pollutant and is believed to be a risk factor for human chemical carcinogenesis, it is important to identify potent naturally occurring/synthetic agents that could modulate B(a)P-induced toxicity. The present study explores the effect of the flavonoid silymarin (2.4mg/ml) in counteracting the toxicity of B(a)P (1μM) in PBMC. Flourimetry and Confocal Laser Scanning Microscopy results showed that silymarin reduces the B(a)P induced ROS production and DNA damage. Atomic Absorption Spectroscopy analysis and fluorescent microscopic pictures proved that silymarin reduces the increased intracellular calcium and apoptosis induction during B(a)P treatment. Furthermore, silymarin did not show any inhibition for CYP1B1 activity at transcriptional level by semiquantitative RT PCR but it affects the catalytic activity of Phase I CYP1A1/CYP1B1 enzyme (EROD assay) during B(a)P treatment. The findings reveal that silymarin possesses substantial protective effect against B(a)P induced DNA damage and calcium mediated apoptosis by inhibiting the catalytic activity of CYP1B1 and maintaining the intracellular calcium dysregulation; hence, it could be considered as a potential protective agent for environmental contaminant induced immunotoxicity.
Department of Biotechnology, Alagappa University, Karaikudi 630 003, Tamil Nadu, India.
Kiruthiga
K
Perumal Vijayaraman
Shanmuganathan
S
Muruganantham
Manickavalli
M
Subramanian
Karutha Pandian
KP
Shunmugiah
Pandima Devi
PD
Kasi
D017209:Apoptosis / Q000187:drug effects*; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects; D004785:Environmental Pollutants / Q000633:toxicity; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012838:Silymarin / Q000494:pharmacology*
D004785:Environmental Pollutants; D020011:Protective Agents; D017382:Reactive Oxygen Species; D012838:Silymarin; D001564:Benzo(a)pyrene; D002118:Calcium