Nonredundant regulation of rice arbuscular mycorrhizal symbiosis by two members of the phosphate transporter1 gene family.
Pi acquisition of crops via arbuscular mycorrhizal (AM) symbiosis is becoming increasingly important due to limited high-grade rock Pi reserves and a demand for environmentally sustainable agriculture. Here, we show that 70% of the overall Pi acquired by rice (Oryza sativa) is delivered via the symbiotic route. To better understand this pathway, we combined genetic, molecular, and physiological approaches to determine the specific functions of two symbiosis-specific members of the PHOSPHATE TRANSPORTER1 (PHT1) gene family from rice, ORYsa;PHT1;11 (PT11) and ORYsa;PHT1;13 (PT13). The PT11 lineage of proteins from mono- and dicotyledons is most closely related to homologs from the ancient moss, indicating an early evolutionary origin. By contrast, PT13 arose in the Poaceae, suggesting that grasses acquired a particular strategy for the acquisition of symbiotic Pi. Surprisingly, mutations in either PT11 or PT13 affected the development of the symbiosis, demonstrating that both genes are important for AM symbiosis. For symbiotic Pi uptake, however, only PT11 is necessary and sufficient. Consequently, our results demonstrate that mycorrhizal rice depends on the AM symbiosis to satisfy its Pi demands, which is mediated by a single functional Pi transporter, PT11.
Department of Plant Molecular Biology, University of Lausane, CH-1015 Lausane, Switzerland.
Shu-Yi
SY
Yang
Mette
M
Grønlund
Iver
I
Jakobsen
Marianne Suter
MS
Grotemeyer
Doris
D
Rentsch
Akio
A
Miyao
Hirohiko
H
Hirochika
Chellian Santhosh
CS
Kumar
Venkatesan
V
Sundaresan
Nicolas
N
Salamin
Sheryl
S
Catausan
Nicolas
N
Mattes
Sigrid
S
Heuer
Uta
U
Paszkowski
D000595:Amino Acid Sequence; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation; D038821:Mycorrhizae / Q000235:genetics* / Q000254:growth & development; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics* / Q000382:microbiology; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013559:Symbiosis / Q000235:genetics*
D028061:Phosphate Transport Proteins; D010940:Plant Proteins