[Protective effects of cerebroside from sea cucumber on the oxidative damage of PC12 cells induced by H2O2].
OBJECTIVE
To study the protective effects of cerebroside from sea cucumber (SCC) on the oxidative damage of PC12 cells induced by H2O2.


METHODS
An oxidative damage model of PC12 cells was induced by H2O2. The survival rate of the cells was determined by MTT method. The leakage of LDH into cell cultures was determined to evaluate the degree of cell damage. The generation of intracellular reactive oxygen species (ROS) was detected by using DCFH-DA fluorescent probe. The activity of total antioxidation capacity (T-AOC) was determined by chemical colorimetric method and the SOD activity was measured by xanthine oxide method.


RESULTS
The injury of PC12 cells induced by H2O2 could be reduced obviously by SCC. Compared with the model group, SCC could improve the survival rate of injured cells, reduce the leakage of LDH into cell culture and reduce intracellular ROS (P < 0.01), increase the intracellular T-AOC and SOD activity (P < 0.01).


CONCLUSIONS
SCC has significantly protective effect on the injured PC12 cells induced by H2O2. The results would provide preliminary data on supporting the development of sea cucumber as a functional food, which might give benefits to our brain.
College of Food Science and Technology, Ocean University of China, Qingdao 266003, China. warmth_hut@yahoo.cn
Fengjuan
F
Wu
Lei
L
Du
Jie
J
Xu
Qingjuan
Q
Tang
Changhu
C
Xue
Yuming
Y
Wang
D000818:Animals; D000975:Antioxidants; D001921:Brain / Q000166:cytology; D002470:Cell Survival; D002554:Cerebrosides / Q000494:pharmacology*; D005452:Fluoresceins; D006861:Hydrogen Peroxide; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species; D012616:Sea Cucumbers
D000975:Antioxidants; D002554:Cerebrosides; D005452:Fluoresceins; D017382:Reactive Oxygen Species; C029569:diacetyldichlorofluorescein; D006861:Hydrogen Peroxide