Enriching C-terminal peptide from endopeptidase ArgC digest for protein C-terminal analysis.
This Letter describes a method for enriching C-terminal peptide of protein for C-terminal sequence analysis. This method employs endopeptidase ArgC digestion and C-terminal peptide enrichment using m-aminophenylboronic acid-agarose as an arginine-capture material. The selectively recovered C-terminal peptide incorporates no artificial derivatization. Therefore, the widely used functional groups (e.g. α-NH(2) and α-COOH) can be used for any necessary transformation. In this research, a TMPP mass tag was attached to the α-NH(2) group to clarify the amino acid sequence of the C-terminal peptide.
Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation, Kyoto 604-8511, Japan. kuyama@shimadzu.co.jp
Hiroki
H
Kuyama
Chihiro
C
Nakajima
Koichi
K
Tanaka
D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology; D001426:Bacterial Proteins / Q000378:metabolism*; D001897:Boronic Acids / Q000737:chemistry; D010455:Peptides / Q000032:analysis*; D011486:Protein C / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012685:Sepharose / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001426:Bacterial Proteins; D001897:Boronic Acids; D010455:Peptides; D011486:Protein C; C028592:3-aminobenzeneboronic acid; D012685:Sepharose; D000445:Aldehyde Oxidoreductases; C089846:argC protein, Bacteria