Altered expression of ezrin, E-Cadherin and β-Catenin in cervical neoplasia.
High-grade cervical squamous intraepithelial lesions (CIN) as well as squamous cell carcinoma and adenocarcinoma of the cervix are associated with persistent high-risk human papillomavirus (HPV) infection. A number of cellular events play a role in HPV pathogenesis and in the development of cervical lesions, including alterations in cell adhesion and motility. The crucial plasma membrane - cytoskeleton linker protein ezrin of the Ezrin-Radixin-Moesin (ERM) protein family is involved in the regulation of cell morphology, cell adhesion and invasion. Based on our previous work on ERM proteins we sought out to study the expression of ezrin in cervical premalignant lesions. We also studied the expression of E-cadherin and β-catenin, which play an important role in epithelial cell adhesion. We observed intensifying expression of ezrin along with progressing grade of neoplasia. Ezrin staining was found to colocalize with p16 staining in high-risk HPV associated lesions. Expression of E-cadherin and β-catenin was found to be altered along with the severity of the lesion, similar to ezrin. Enhanced expression of ezrin in cervical HPV associated lesions suggests a role in the development of cervical neoplasia and cancer. Further clinical evaluation should reveal the feasibility of ezrin as a biomarker for the progression of cervical lesions.
Department of Virology, University of Helsinki, Helsinki, Finland. eeva.auvinen@helsinki.fi
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Auvinen
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Carpen
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Korpela
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M
Ronty
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Vaheri
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Tarkkanen
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism*; D002584:Cervix Uteri / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D060787:Neoplasm Grading; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000378:metabolism / Q000473:pathology / Q000821:virology; D011379:Prognosis; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D051176:beta Catenin / Q000378:metabolism*; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D000099149:Ezrin
D014408:Biomarkers, Tumor; D015820:Cadherins; D003598:Cytoskeletal Proteins; D004279:DNA, Viral; D051176:beta Catenin; D000099149:Ezrin; C495270:CTNNB1 protein, human