Wound healing activity of latex of Euphorbia caducifolia.
BACKGROUND
[corrected] Euphorbia caducifolia is a Euphorbiaceae species native to Thar Desert of India, where latex of E. caducifolia (ECL) is used by the local inhabitants for treatment of bleeding wound, cutaneous eruption and other skin diseases. Looking to the medicinal importance of the plant, it was considered worthwhile to scientifically evaluate the ECL for the wound healing activity.


METHODS
In vitro methods, clotting of platelet free plasma and angiogenesis in chick chorioallantoic membrane (CCM) were used for studying effect of ECL on clotting and angiogenesis. Excision and incision wounds model were used to study effect of ECL on wound contraction, tensile strength and hydroxyproline and DNA content.


RESULTS
ECL treatment stimulates the blood vessel formation in CCM, at the all dose levels. 56.77, 74.48 and 78.09% increase in area of angiogenesis was observed in ECL 2.5, 5.0 and 10mg/ml treated groups respectively. The excised skin of ECL 5.0 and 10mg/ml treated animals found to have higher content of hydroxyproline and DNA, when compared with control group. An acceleration of the wound closure was observed with an enhanced number of fibroblasts and collagen content in ECL treated mice.


CONCLUSIONS
Result of in vitro and in vivo experiments indicate that ECL exerts significant wound healing activity, demonstrating its relevant therapeutic potential.
Lachoo Memorial College of Science and Technology, Pharmacy Wing, Jodhpur 342008, Rajasthan, India. manojgoyal620@yahoo.com
Manoj
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Goyal
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BP
Nagori
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Sasmal
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects; D004247:DNA / Q000032:analysis; D028481:Euphorbia; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis; D007194:India; D007840:Latex / Q000494:pharmacology*; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects; D051381:Rats; D012867:Skin / Q000293:injuries / Q000378:metabolism; D013718:Tensile Strength; D023382:Toxicity Tests, Acute; D014945:Wound Healing / Q000187:drug effects*
D007840:Latex; D004247:DNA; D006909:Hydroxyproline