Inhibition of reverse transcriptase activity increases stability of the HIV-1 core.
Previous studies showed that HIV-1 reverse transcription occurs during or before uncoating, linking mechanistically reverse transcription with uncoating. Here we show that inhibition of reverse transcriptase (RT) during HIV-1 infection by pharmacologic or genetic means increased the stability of the HIV-1 core during infection. Interestingly, HIV-1 particles with increased core stability were resistant to the core-destabilizing effects of rhesus TRIM5α (TRIM5α(rh)). Collectively, this work implies that the surface of the HIV-1 core is dynamic and changes upon the ongoing processes within the core.
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, USA.
Yang
Y
Yang
Thomas
T
Fricke
Felipe
F
Diaz-Griffero
D000818:Animals; D002460:Cell Line; D004285:Dogs; D054303:HIV Reverse Transcriptase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D015497:HIV-1 / Q000201:enzymology* / Q000502:physiology*; D048348:Reverse Transcription; D016662:Virus Integration
C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase