Vaccination with recombinant actin from scab mites and evaluation of its protective efficacy against Psoroptes cuniculi infection.
The mite Psoroptes cuniculi is globally widespread and has a serious impact on commercial rabbit breeding. Current treatment methods are based on chemotherapy. Because of the disadvantages of these methods, alternative measures are required, and vaccination is one of the most promising strategies. Here, we cloned and expressed the recombinant P. cuniculi actin gene (rPc-act). Antiserum levels against rPc-act in rabbits were used to locate actin distribution in mite sections. Challenge trials were carried out to evaluate the immunity protection of rPc-act in rabbits, with antibody levels determined by ELISA. Sequence analysis of this gene fragment showed 89·26% and 84·91% identity to Sarcoptes scabiei and Mayetiola destructor sequences, respectively. Immunohistochemistry showed rPc-act to locate widely throughout the mites, especially in feet and muscle tissues. Recombinant P. cuniculi actin with QuliA adjuvant was used to immunize six rabbits. Each animal was challenge-infested with 25-50 adult mites. Although IgE levels showed no significant difference to controls, IgG levels were significantly higher, and clinical development showed no significantly different severity of lesions in vaccinated rabbits than in the controls. This study showed that rPc-act is a muscular isotype actin and has no clinical protective efficacy against P. cuniculi.
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Yaan, China.
W
W
Zheng
Q
Q
Tang
R
R
Zhang
Q
Q
Jise
Y
Y
Ren
X
X
Nong
X
X
Wu
X
X
Gu
S
S
Wang
X
X
Peng
S
S
Lai
G
G
Yang
D000199:Actins / Q000235:genetics / Q000276:immunology*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008924:Mite Infestations / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control / Q000662:veterinary*; D008969:Molecular Sequence Data; D010802:Phylogeny; D040042:Psoroptidae / Q000276:immunology*; D011817:Rabbits / Q000276:immunology* / Q000469:parasitology*; D011897:Random Allocation; D016415:Sequence Alignment; D014611:Vaccination / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
D000199:Actins; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; D007073:Immunoglobulin E