Transcriptional silencing by single-stranded RNAs targeting a noncoding RNA that overlaps a gene promoter.
RNAi using single-strand RNA would provide new options for therapeutic development and for investigating critical questions of mechanism. Using chemically modified single-strands, we test the hypothesis that single-stranded RNAs can engage the RNAi pathway and silence gene transcription. We find that a chemically modified single-stranded silencing RNA (ss-siRNA) designed to be complementary to a long noncoding RNA (lncRNA) requires argonaute protein, functions through the RNAi pathway, and inhibits gene transcription. These data expand the use of single-stranded RNA to cell nuclei.
Masayuki
M
Matsui
Thazha P
TP
Prakash
David R
DR
Corey
D000818:Animals; D015153:Blotting, Western; D020868:Gene Silencing; D008954:Models, Biological; D011401:Promoter Regions, Genetic / Q000187:drug effects* / Q000235:genetics; D034741:RNA, Small Interfering / Q000494:pharmacology*; D022661:RNA, Untranslated / Q000187:drug effects*; D015533:Transcriptional Activation / Q000187:drug effects
D034741:RNA, Small Interfering; D022661:RNA, Untranslated