Structural characterization and radioprotection of bone marrow hematopoiesis of two novel polysaccharides from the root of Angelica sinensis (Oliv.) Diels.
Two novel homogeneous polysaccharides, APS-1a and APS-3a were successfully isolated from the root of Angelica sinensis. APS-1a was composed of galactose, arabinose and glucose in a relatively molar percentage of 57.34%, 27.67% and 14.98%, and had a molecular weight of 49.0kDa, whereas APS-3a was composed of galactose, arabinose and glucose in a relatively molar percentage of 84.54%, 6.50%, and 8.96%, and had a molecular weight of 65.4kDa. APS-1a and APS-3a mainly consisted of 1,4-linked galactose, 1,3,6-linked galactose, T-galactose and T-arabinose, and the molar ratio of each linkage was different between APS-1a and APS-3a. The bioactivity analysis showed that APS-1a and APS-3a increased the thymus and spleen index, the number of red blood cell (RBC) and white blood cell (WBC) in peripheral blood and the cellularity of bone marrow cell numbers in irradiated mice, protected mice against radiation-induced micronucleus formation in bone marrow, suggesting that polysaccharides could be used as radioprotective agents, especially for promoting bone marrow hematopoiesis.
School of Pharmacy, Second Military Medical University, Shanghai, 200433, China.
Lu
L
Zhao
Yin
Y
Wang
Han-lin
HL
Shen
Xu-dong
XD
Shen
Yan
Y
Nie
Ying
Y
Wang
Ting
T
Han
Ming
M
Yin
Qiao-yan
QY
Zhang
D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D005720:Gamma Rays / Q000009:adverse effects*; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology; D011897:Random Allocation
C581172:APS-1a polysaccharide; C581173:APS-3a polysaccharide; D011134:Polysaccharides; D011837:Radiation-Protective Agents