Measurements of cytosolic ion concentrations in live cells.
Here, we describe a series of methods suitable for the measurement of cytosolic ion concentrations in living plant cells using ion selective dyes. We describe procedures for the use of SBFI for the measurement of Na(+) in live cells. The resulting material is suitable for most standard cell biology procedures.
Department of (Agri) Biotechnology, College of Agriculture-Hassan, Subcampus of University of Agricultural Sciences, Bangalore, India.
Veena S
VS
Anil
P G
PG
Kavitha
Sam
S
Kuruvilla
Pavan
P
Kumar
M K
MK
Mathew
D001572:Benzofurans / Q000378:metabolism; D002470:Cell Survival; D003600:Cytosol / Q000378:metabolism*; D004988:Ethers, Cyclic / Q000378:metabolism; D007477:Ions; D036641:Microscopy, Fluorescence, Multiphoton; D012275:Oryza / Q000166:cytology* / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism*; D018520:Plant Shoots / Q000166:cytology / Q000378:metabolism; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*
D001572:Benzofurans; D004988:Ethers, Cyclic; D007477:Ions; C061665:sodium-binding benzofuran isophthalate; D012964:Sodium