Characterization of low-pathogenic H6N6 avian influenza viruses in central China.
Three strains of H6N6 subtype avian influenza virus (AIV) were isolated from live-poultry markets of central China during 2009-2010. A phylogenetic analysis showed that these isolates originated from gene reassortment among different virus lineages of the H6 subtype. In an experimental infection of animals, the selected isolate was non-pathogenic for chickens and low-pathogenic for mice. The wild-type isolate was capable of replication in mouse lung without prior adaptation, and the virulence to mice increased rapidly during adaption in mouse lung. The genomes of viruses of passage 0 (P0), P4, and P8 were sequenced and compared, and virulence-related amino acid substitutions were found in multiple sites during mouse lung passage.
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, Hubei, China.
Yanfeng
Y
Yao
Huadong
H
Wang
Quanjiao
Q
Chen
Hongbo
H
Zhang
Tao
T
Zhang
Jianjun
J
Chen
Bing
B
Xu
Hanzhong
H
Wang
Bing
B
Sun
Ze
Z
Chen
D000220:Adaptation, Biological; D000818:Animals; D002645:Chickens; D002681:China; D016000:Cluster Analysis; D004252:DNA Mutational Analysis; D004195:Disease Models, Animal; D009980:Influenza A virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000821:virology*; D008168:Lung / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D017422:Sequence Analysis, DNA; D014774:Virulence
D012367:RNA, Viral