Manipulation of Ralstonia eutropha carbon storage pathways to produce useful bio-based products.
Ralstonia eutrophais a Gram-negative betaproteobacterium found natively in soils that can utilize a wide array of carbon sources for growth, and can store carbon intracellularly in the form of polyhydroxyalkanoate. Many aspects of R. eutrophamake it a good candidate for use in biotechnological production of polyhydroxyalkanoate and other bio-based, value added compounds. Manipulation of the organism's carbon flux is a cornerstone to success in developing it as a biotechnologically relevant organism. Here, we examine the methods of controlling and adapting the flow of carbon in R. eutrophametabolism and the wide range of compounds that can be synthesized as a result. The presence of many different carbon utilization pathways and the custom genetic toolkit for manipulation of those pathways gives R. eutrophaa versatility that allows it to be a biotechnologically important organism.
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.
Christopher J
CJ
Brigham
Natalia
N
Zhila
Ekaterina
E
Shishatskaya
Tatiana G
TG
Volova
Anthony J
AJ
Sinskey
D001688:Biological Products / Q000378:metabolism*; D001709:Biotechnology; D002244:Carbon / Q000378:metabolism*; D020118:Cupriavidus necator / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis
D001688:Biological Products; D054813:Polyhydroxyalkanoates; D002244:Carbon