Selection of hepatocyte-like cells from mouse differentiated embryonic stem cells and application in therapeutic liver repopulation.
BACKGROUD/AIM: Because of the oncogenic risk, it is important to gain the homogeneous and purified cells from differentiated ESCs before transplantation. Here, we aim to select hepatocyte-like cells from differentiated ESCs, and investigate their growth, differentiation and neoplastic formation after intrahepatic transplantation.


METHODS
Mouse ESCs were primarily induced by Dexamethesone, FGF-4 and HGF sequentially, then placed to a conditioning selection media consisting of 5% cholestatic sera and cultivated for 2 wks. After labeled by CFDA-SE, the selected cells were transplanted into mouse liver in therapeutic liver repopulation models.


RESULTS
In the early stage of screening cultivation, most cells were suffered from apoptosis or even death. 1w later, some hepatocyte-like colony-forming units were observed, then the selected cells could grow and tend to be more mature, as assessed by morphological and functional tests. After intrahepatic transplantation, the labeled cells could proliferate and expressed albumin. Moreover, teratoma didn't form over 3 months.


CONCLUSIONS
Our conditioning selection media could not only effectively select hepatocyte-like cells from differentiated ESCs, but further promote their growth and differentiation as well. After intrahepatic transplantation in therapeutic liver repopulation models, the selected cells could grow, differentiate and keep partial hepatic function. In particular, the transplantation was safe.
Department of Pediatric Surgery, The Memorial Hospital of Sun Yat-Sen University, Guangzhou, China. dengxiaogeng@yahoo.com.cn
Xiao-Geng
XG
Deng
Rong-Lin
RL
Qiu
Zhi-Xi
ZX
Li
Jie
J
Zhang
Jia-Jia
JJ
Zhou
Yao-Hao
YH
Wu
Le-Xiang
LX
Zeng
Jing
J
Tang
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D051519:Fibroblast Growth Factor 4 / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects / Q000637:transplantation*; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008115:Liver Regeneration; D016031:Liver Transplantation; D051379:Mice; D008815:Mice, Inbred Strains; D011763:Pyrrolizidine Alkaloids / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D051519:Fibroblast Growth Factor 4; D011763:Pyrrolizidine Alkaloids; D017228:Hepatocyte Growth Factor; D003907:Dexamethasone; C003300:retrorsine