Biochemical and biophysical response to calcium chloride stress in Aspergillus niger and its role in malachite green degradation.
Filamentous fungi show great promise in remediation of environmental contaminants such as industrial dyes. In the current study, Aspergillus niger (Genbank ID: JF437542) decolorized 82 % of the test dye malachite green (MG; 50 mg/l) during cultivation for 24 h. The organism decolorized only 6 % of the MG at higher concentration (250 mg MG/l) during the same time period and growth was inhibited at this higher MG concentration. Exposing A. niger to different types of stress resulted in variable impacts on ability to decolorize MG. CaCl2 had the largest positive impact on decolorization. A. niger cultures treated with CaCl2 (1 M) decolorized 46 % of the MG (250 mg/l) in 1 h compared to 6 % in untreated control cultures. CaCl2 also increased catalase production in A. niger which strongly supported a direct relationship between stress response and decolorizing ability. Spectrophotometric measurement confirmed MG decolorization while Fourier transform infrared spectroscopy suggested that biodegradation of MG occurred. Cultures treated with CaCl2 accumulated fewer toxic MG by-products than untreated cultures. CaCl2-induced stress increased the permeability and conductivity of the fungal cell membrane. An observed increase in medium [H(+)] also suggested a change in Ca(2+)/H(+) exchange capacity in the fungal cell. Calcium ions had a pronounced effect on membrane properties and this may have had an important impact on signal transduction. We conclude that A. niger decolorizes MG and that CaCl2 enhances this process; the CaCl2 effect appears to be associated with stress response.
Microbiology Department, National Center for Radiation Research and Technology (NCRRT), Atomic Energy Authority, 3 Ahmad El Zomor St., Nasr City, Cairo, 11371, Egypt. ola_gomaa@hotmail.com
Ola M
OM
Gomaa
Nabila S
NS
Selim
John E
JE
Linz
D001234:Aspergillus niger / Q000145:classification / Q000187:drug effects* / Q000378:metabolism; D001673:Biodegradation, Environmental; D002122:Calcium Chloride / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010802:Phylogeny; D012394:Rosaniline Dyes / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013312:Stress, Physiological
D005656:Fungal Proteins; D012394:Rosaniline Dyes; C005095:malachite green; D002374:Catalase; D002122:Calcium Chloride