Polymorphism in a histone H1 subtype with a short N-terminal domain in three legume species (Fabaceae, Fabaeae).
A number of alleles of an orthologous gene His6 encoding histone H1 subtype f (H1-6 in pea) accumulated in chromatin of old tissues were sequenced in three legume species: seven alleles in Pisum sativum, four in Vicia unijuga and eight in Lathyrus gmelinii. In the total of 19 alleles sequenced in the three species, 29 non-synonymous substitutions and six indels were found in the coding region; most of amino acid substitutions (26 of 29) and all indels occurred in the C-terminal hydrophilic domain of the encoded protein. All species were polymorphic for some non-synonymous substitutions, V. unijuga was also polymorphic for one and P. sativum for two indels. Three near-isogenic lines of P. sativum bearing different alleles showed differences in many quantitative traits; that in the growth dynamic could be tentatively attributed to the allelic substitution of subtype H1-6. The frequencies of four electromorphs in a sampled locality of V. unijuga were found to be close to those observed 25 years ago, although their rapid change in the past was supposed in the previous study.
Institute of Cytology and Genetics SB RAS, Acad. Lavrentyev Ave 10, Novosibirsk, Russia, 630090. kosterin@bionet.nsc.ru
Oleg E
OE
Kosterin
Vera S
VS
Bogdanova
Andrey A
AA
Kechin
Olga O
OO
Zaytseva
Arseniy K
AK
Yadrikhinskiy
D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D007887:Fabaceae / Q000033:anatomy & histology / Q000235:genetics*; D006657:Histones / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D054643:INDEL Mutation / Q000235:genetics; D007438:Introns / Q000235:genetics; D027807:Lathyrus / Q000033:anatomy & histology / Q000235:genetics; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000235:genetics; D018532:Pisum sativum / Q000033:anatomy & histology / Q000235:genetics; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D019655:Quantitative Trait, Heritable; D016415:Sequence Alignment; D013045:Species Specificity; D031306:Vicia / Q000033:anatomy & histology / Q000235:genetics
D006657:Histones; D009711:Nucleotides