Reduced lifetimes are directly correlated with excitation irradiance in metal-enhanced fluorescence (MEF).
We describe a fundamental observation in Metal-Enhanced Fluorescence (MEF), which has become a leading technology in the life sciences today, namely, how the lifetime of fluorophores near-to metallic plasmon-supporting silver islands/nanoparticles, modulates as a function of excitation power irradiance. This finding is in stark contrast to that observed in classical far-field fluorescence spectroscopy, where excitation power does not influence fluorophore radiative decay/lifetime.
Institute of Fluorescence and Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD 21202, USA.
Jan O
JO
Karolin
Chris D
CD
Geddes
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000737:chemistry; D056831:Coordination Complexes; D053768:Metal Nanoparticles / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D056831:Coordination Complexes; D012834:Silver; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds