[Study on animal model of testing food allergenicity in BN rat].
OBJECTIVE
To establish a BN rat model for testing food allergenicity.


METHODS
BN rats of both sex and aged 4 and 8 weeks were exposed to different doses of ovalbumin (OVA) (1.00 mg, 0.10 mg and 0.01 mg) by intraperitoneal injection on the 1st, 5th and 10th day of the study and observed for 35 days. Blood samples were taken on the 28th and 35th day of the study from orbital plexus and serum specific IgE (OVA sIgE) were determined by ELISA.


RESULTS
Compared with the control group, the concentrations of OVA sIgE of 8-week female BN rats on the 28th and 35th day were significantly increased when 1.00 mg and 0.10 mg OVA was applied (P < 0.05). The concentrations of OVA sIgE of 8-week male BN rats on the 28th day were significantly higher than that in the control group when 0.10 mg and 0.01 mg OVA was applied (P < 0.05). And the concentrations of OVA sIgE of 8-week male BN rats on the 35th day were significantly higher than that in the control group when 1.00 mg OVA was applied (P < 0.05). The concentrations of OVA sIgE of 4-week male BN rats on the 35th day were significantly higher than the control group when 1.00 mg OVA was applied (P < 0.05).


CONCLUSIONS
Female rats were more sensitive than male rats to different doses of OVA administered intraperitoneally; 8-week rats were more sensitive than 4-week rats; the better dose of OVA for sensitizing BN rats was 0.10 mg or 1.00 mg. Therefore, an ideally sensitized animal model could be established by administering 8-week female BN rats with 0.10 mg or 1.00 mg OVA intraperitoneally in 35 days later.
National Institute for Nutrition and Food Safety, China CDC, Beijing 100050, China. sunnana28@yahoo.com
Nana
N
Sun
Chunlai
C
Liang
Qiannan
Q
Zhang
Xin
X
Zhang
Wenming
W
Cui
Wei
W
Wang
Xudong
X
Jia
D000284:Administration, Oral; D000485:Allergens; D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005512:Food Hypersensitivity; D006801:Humans; D007073:Immunoglobulin E; D008297:Male; D010047:Ovalbumin; D051381:Rats; D011914:Rats, Inbred BN
D000485:Allergens; D007073:Immunoglobulin E; D010047:Ovalbumin