Functional protein microarrays by electrohydrodynamic jet printing.
This paper reports the use of advanced forms of electrohydrodynamic jet (e-jet) printing for creating micro- and nanoscale patterns of proteins on various surfaces ranging from flat silica substrates to structured plasmonic crystals, suitable for micro/nanoarray analysis and other applications in both fluorescent and plasmonic detection modes. The approaches function well with diverse classes of proteins, including streptavidin, IgG, fibrinogen, and γ-globulin. Detailed study reveals that the printing process does not adversely alter the protein structure or function, as demonstrated in the specific case of streptavidin through measurements of its binding specificity to biotin-modified DNA. Multinozzle printing systems enable several types of proteins (up to four currently) to be patterned on a single substrate, in rapid fashion and with excellent control over spatial dimensions and registration. High-speed, pulsed operational modes allow large-area printing, with narrow statistical distributions of drop size and spacing in patterns that include millions of droplets. The process is also compatible with the structured surfaces of plasmonic crystal substrates to enable detection without fluorescence. These collective characteristics suggest potential utility of e-jet techniques in wide-ranging areas of biotechnology, where its compatibility with various biomaterials and substrates with different topographies and surface chemistries, and ability to form deposits that range from thick films to submonolayer coatings, derive from the remote, noncontacting physical material transfer mode of operation.
Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
Kazuyo
K
Shigeta
Ying
Y
He
Erick
E
Sutanto
Somi
S
Kang
An-Phong
AP
Le
Ralph G
RG
Nuzzo
Andrew G
AG
Alleyne
Placid M
PM
Ferreira
Yi
Y
Lu
John A
JA
Rogers
D000818:Animals; D004560:Electricity; D057446:Hydrodynamics; D007074:Immunoglobulin G / Q000378:metabolism; D011327:Printing / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*
D007074:Immunoglobulin G