Fusion of gemini based cationic liposomes with cell membrane models: implications for their biological activity.
The interaction of neutral and anionic phospholipid liposomes, used as cell models, with cationic liposomes formulated with 1,2-dimyristoyl-sn-glicero-3-phosphocholine and stereomeric cationic gemini surfactants was investigated by differential scanning calorimetry, fluorescence experiments and dynamic laser light scattering. This study was aimed at rationalizing the different biological features shown by liposomes based on different gemini stereoisomers observed in previous investigations. In fact, to correlate the observed biological activity of liposomes with the molecular structure of their components is critical for a rational and systematic approach to the design of new carriers for drug delivery. The obtained results show that the different stereochemistry of the gemini surfactant controls the interaction and the extent of fusion with different cell models.
Dipartimento di Chimica Università degli Studi di Roma "Sapienza", P.le A. Moro 5, 00185 Roma, Italy.
Simone
S
Aleandri
Cecilia
C
Bombelli
Maria Grazia
MG
Bonicelli
Federico
F
Bordi
Luisa
L
Giansanti
Giovanna
G
Mancini
Marco
M
Ierino
Simona
S
Sennato
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D001692:Biological Transport; D002152:Calorimetry, Differential Scanning / Q000379:methods; D002412:Cations; D002462:Cell Membrane / Q000378:metabolism*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D004337:Drug Carriers; D016503:Drug Delivery Systems; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry; D008956:Models, Chemical; D010715:Phosphatidylglycerols / Q000737:chemistry; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature
D002412:Cations; D004337:Drug Carriers; D008051:Lipid Bilayers; D008081:Liposomes; D010715:Phosphatidylglycerols; D013501:Surface-Active Agents; D015060:1,2-Dipalmitoylphosphatidylcholine; D004134:Dimyristoylphosphatidylcholine; C030345:1,2-dipalmitoylphosphatidylglycerol