Specific association of lectin LecB with the surface of Pseudomonas aeruginosa: role of outer membrane protein OprF.
The fucose binding lectin LecB affects biofilm formation and is involved in pathogenicity of Pseudomonas aeruginosa. LecB resides in the outer membrane and can be released specifically by treatment of an outer membrane fraction with fucose suggesting that it binds to specific ligands. Here, we report that LecB binds to the outer membrane protein OprF. In an OprF-deficient P. aeruginosa mutant, LecB is no longer detectable in the membrane but instead in the culture supernatant indicating a specific interaction between LecB and OprF.
Institute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Juelich, Germany.
Horst
H
Funken
Kai-Malte
KM
Bartels
Susanne
S
Wilhelm
Melanie
M
Brocker
Michael
M
Bott
Manjeet
M
Bains
Robert E W
RE
Hancock
Frank
F
Rosenau
Karl-Erich
KE
Jaeger
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms; D006384:Hemagglutination; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000166:cytology* / Q000378:metabolism* / Q000502:physiology; D013379:Substrate Specificity
D001426:Bacterial Proteins; D055767:Immobilized Proteins; C534600:LecB protein, Pseudomonas aeruginosa; D037102:Lectins; C547605:OprF protein, Pseudomonas aeruginosa