Transition pattern and mechanism of B-lymphocyte precursors in regenerated mouse bone marrow after subtotal body irradiation.
Little is known about the effects of ionizing radiation on the transition and the related signal transduction of progenitor B cells in the bone marrow. Thus, using an NIH Swiss mouse model, we explored the impact of ionizing radiation on the early stage of B-cell development via an examination of the transition of CLP to pro-B to pre-B cells within bone marrow as a function of radiation doses and times. Our results showed that while the total number of bone marrow lymphoid cells at different stages were greatly reduced by subtotal body irradiation (sub-TBI), the surviving cells continued to transition from common lymphoid progenitors to pro-B and then to pre-B in a reproducible temporal pattern. The rearrangement of the immunoglobulin heavy chain increased significantly 1-2 weeks after irradiation, but no change occurred after 3-4 weeks. The rearrangement of the immunoglobulin light chain decreased significantly 1-2 weeks after sub-TBI but increased dramatically after 3-4 weeks. In addition, several key transcription factors and signaling pathways were involved in B-precursor transitions after sub-TBI. The data indicate that week 2 after irradiation is a critical time for the transition from pro-B cells to pre-B cells, reflecting that the functional processes for different B-cell stages are well preserved even after high-dose irradiation.
First Affiliated Hospital, Fujian Medical University, Fuzhou, Fujian, China. hdp20012004@163.com
Deping
D
Han
Mei
M
Zhang
Jun
J
Ma
Jingshen
J
Hong
Chun
C
Chen
Bingrong
B
Zhang
Luqiang
L
Huang
Wenlong
W
Lv
Liangjie
L
Yin
Amy
A
Zhang
Hengshan
H
Zhang
Zhenhuan
Z
Zhang
Sadasivan
S
Vidyasagar
Paul
P
Okunieff
Lurong
L
Zhang
D000818:Animals; D001853:Bone Marrow / Q000502:physiology* / Q000528:radiation effects*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D002452:Cell Count; D015322:Gene Rearrangement, B-Lymphocyte; D008297:Male; D051379:Mice; D054448:Precursor Cells, B-Lymphoid / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D020395:Receptors, Interleukin-7 / Q000378:metabolism; D012038:Regeneration / Q000528:radiation effects*; D015398:Signal Transduction / Q000528:radiation effects; D014157:Transcription Factors / Q000378:metabolism; D014916:Whole-Body Irradiation
D020395:Receptors, Interleukin-7; D014157:Transcription Factors