Highly sensitive fluorescence detection of avidin/streptavidin with an optical interference mirror slide.
This paper presents highly sensitive fluorescence detections of avidin and streptavidin using an optical interference mirror (OIM) slide consisting of a plane mirror covered with an optical interference layer.  Compared with a common glass slide, the OIM slide can enhance the fluorescence from a dye by more than 100-fold.  We fabricated an OIM slide by depositing an optical interference layer of Al(2)O(3) on an Ag mirror.  To enhance the fluorescence maximally, the optimal thickness of the Al(2)O(3) layer was estimated from optical interference theory.  For detections of protein, avidin/streptavidin labeled with fluorescein, Cy3, and Cy5 were detected with biotin immobilized on an OIM slide with the optimal Al(2)O(3) thickness.  We achieved a sensitivity improvement of more than 50-fold, comparing with a glass slide.  Such a high degree of improvement would be a significant contribution to further progress in biomedical research and medical diagnostics.
School of Bioscience and Biotechnology, Tokyo University of Technology, Hachioji, Tokyo 192–0982, Japan.
Mitsuru
M
Yasuda
Takuo
T
Akimoto
D000327:Adsorption; D000537:Aluminum Oxide / Q000737:chemistry; D001360:Avidin / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D002232:Carbocyanines / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism; D055096:Optical Devices; D012834:Silver / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D019809:Streptavidin / Q000032:analysis* / Q000378:metabolism
D002232:Carbocyanines; D055767:Immobilized Proteins; D012996:Solutions; C089187:cyanine dye 3; C085321:cyanine dye 5; D001360:Avidin; D012834:Silver; D001710:Biotin; D019809:Streptavidin; D000537:Aluminum Oxide