Real-time PCRs assay for serogrouping Listeria monocytogenes and differentiation from other Listeria spp.
A 5'-exonuclease real-time triplex-PCR assay was developed for serogrouping Listeria monocytogenes, and differentiation from other Listeria spp. The assay was evaluated on 109 Listeria cultures, and results were compared with a previously validated gel-based multiplex-PCR procedure. All L. monocytogenes were correctly classified into four serogroups, including atypical serotype 4b strains, and differentiated from other Listeria species. The assay is a rapid method for categorisation of suspect L. monocytogenes.
Department of Medicine and of Health Sciences, University of Molise, Campobasso, Italy.
Monia
M
Vitullo
Kathie Ann
KA
Grant
Michela Lucia
ML
Sammarco
Manuela
M
Tamburro
Giancarlo
G
Ripabelli
Corinne Francoise Laurence
CF
Amar
D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping / Q000379:methods; D013045:Species Specificity
D001426:Bacterial Proteins; D004269:DNA, Bacterial