Metabolic engineering of inducer formation for cellulase and hemicellulase gene expression in Trichoderma reesei.
The filamentous fungus T. reeseiis today a paradigm for the commercial scale production of different plant cell wall degrading enzymes mainly cellulases and hemicellulases. Its enzymes have a long history of safe use in industry and well established applications are found within the pulp, paper, food, feed or textile processing industries. However, when these enzymes are to be used for the saccharification of cellulosic plant biomass to simple sugars which can be further converted to biofuels or other biorefinery products, and thus compete with chemicals produced from fossil sources, additional efforts are needed to reduce costs and maximize yield and efficiency of the produced enzyme mixtures. One approach to this end is the use of genetic engineering to manipulate the biochemical and regulatory pathways that operate during enzyme production and control enzyme yield. This review aims at a description of the state of art in this area.
Research Division Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorferstraße 1a, 166-5, A-1060, Vienna, Austria.
Bernhard
B
Seiboth
Silvia
S
Herold
Christian P
CP
Kubicek
D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D004790:Enzyme Induction / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005809:Genes, Regulator; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D006868:Hydrolysis; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics
D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase