Estimation of cell membrane properties and erythrocyte red-ox balance in patients with metabolic syndrome.
Metabolic syndrome (MS) is associated with occurrence of the many cardiovascular risk factors such as atherogenic dyslipidemia, visceral fat distribution, arterial hypertension and pro-thrombotic and pro-inflammatory status. In our study the effect of disorders that appear in MS on red-ox balance and erythrocyte cell membrane properties were estimated. The study comprised 50 patients with diagnosed MS and in 25 healthy subjects. Content of thiobarbituric acid reactive substances (TBARS) and catalase, superoxide dismutase and glutathione peroxidase activity were estimated in red blood cells. Moreover, conformation status of membrane proteins, membrane fluidity and osmotic fragility were evaluated. MS was found to manifest: (1) the increase of the concentration of TBARS in erythrocytes with no statistically significant differences in antioxidant enzymes activity, (2) disorders in the structure of erythrocyte cytoskeleton proteins, (3) the increase in membrane lipids fluidity at the depth of 5th and 12th carbon atom of fatty acid hydrocarbon chain and significantly decreased fluidity at the depth of 16th carbon atom, (4) increased erythrocyte osmotic fragility.
Department of Pharmacology and Toxicology, Medical University of Lodz, Lodz, Poland.
Edward
E
Kowalczyk
Jan
J
Kowalski
Jan
J
Błaszczyk
Łukasz
Ł
Gwoździński
Julita
J
Ciećwierz
Monika
M
Sienkiewicz
D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007460:Iodoacetamide / Q000378:metabolism; D008297:Male; D008301:Maleimides / Q000378:metabolism; D024821:Metabolic Syndrome / Q000378:metabolism* / Q000473:pathology*; D008875:Middle Aged; D012399:Rotation; D013997:Time Factors; D055815:Young Adult
D008301:Maleimides; C043592:maleimide; D007460:Iodoacetamide