A highly sensitive and selective immunoassay for the detection of tetrabromobisphenol A in soil and sediment.
Tetrabromobisphenol A is the most widely used brominated flame retardant. A sensitive and selective enzyme-linked immunosorbent assay (ELISA) for the detection of tetrabromobisphenol A was developed. The limit of detection and the inhibition half-maximum concentration of tetrabromobisphenol A in phosphate buffered saline with 10% methanol were 0.05 and 0.87 ng mL(-1), respectively. Cross-reactivity values of the ELISA with a set of important brominated flame retardants including tetrabromobisphenol A-bis(2,3-dibromopropylether), 2,2',6,6'-tetrabromobisphenol A diallyl ether, hexabromocyclododecane, 1,2-bis(pentabromodiphenyl) ethane, 1,2-bis(2,4,6 tribromophenoxy) ethane, bis(2-ethylhexyl)-3,4,5,6-tetrabromophthalate, 2-ethylhexyl-2,3,4,5-tetrabromobenzoate, and polybrominated diphenyl ethers were <0.05%. Concentrations of tetrabromobisphenol A determined by ELISA in the soils from farmlands, the soils from an e-waste recycling site, and the sediments of a canal were in the range of non-detectable-5.6 ng g(-1), 26-104 ng g(-1) and 0.3-22 ng g(-1)dw, respectively, indicating the ubiquitous pollution of tetrabromobisphenol A. The results of this assay for 16 real world samples agreed well with those of the liquid chromatography-tandem mass spectrometry method, indicating this ELISA is suitable for screening of tetrabromobisphenol A in environmental matrices.
College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Ting
T
Xu
Jia
J
Wang
Shang-zhong
SZ
Liu
Cong
C
Lü
Weilin L
WL
Shelver
Qing X
QX
Li
Ji
J
Li
D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005411:Flame Retardants / Q000032:analysis*; D019015:Geologic Sediments / Q000032:analysis*; D057230:Limit of Detection; D011075:Polybrominated Biphenyls / Q000032:analysis* / Q000276:immunology; D012987:Soil / Q000032:analysis*
D005411:Flame Retardants; D011075:Polybrominated Biphenyls; D012987:Soil; C020806:tetrabromobisphenol A