Regulatory effects of apoptosis-associated speck-like protein on cytokines in the P388D1 macrophage-like cell line.
Apoptosis-associated speck-like protein (ASC) is an adaptor molecule of caspase-1 activation that stimulates the secretion of multiple pro-inflammatory cytokines. We investigated the regulatory effects of ASC in the P388D1 macrophage-like cell line. Data showed that ASC overexpression induced by pEGFP-ASC-C2 transfection significantly increased caspase-1 expression and interleukin (IL)-1β and IL-6 secretion, but did not affect tumor necrosis factor (TNF)-α secretion. However, siRNA against ASC significantly decreased the caspase-1 expression and IL-1β and IL-6 secretion, but did not affect TNF-α secretion in the P388D1 cells. We suggest that ASC acts as an inflammatory response-associated gene by regulating caspase-1 activation and IL-1β and IL-6 secretion, which may correlate with its biological effects.
Department of Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116023, PR China.
Shuangyi
S
Ren
Hailong
H
Chen
D000818:Animals; D051017:Apoptosis Regulatory Proteins; D053468:CARD Signaling Adaptor Proteins; D020170:Caspase 1 / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation / Q000235:genetics; D015870:Gene Expression; D005786:Gene Expression Regulation; D020868:Gene Silencing; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D034622:RNA Interference
D051017:Apoptosis Regulatory Proteins; D053468:CARD Signaling Adaptor Proteins; D016207:Cytokines; D003598:Cytoskeletal Proteins; C498534:Pycard protein, mouse; D020170:Caspase 1