Synthesis of cyclic peptides containing a thioester handle for native chemical ligation.
The synthesis of cyclic peptides containing a thioester handle using a sulfo-click linker is reported. These cyclic peptides can be coupled to N-terminal cysteine-containing constructs via native chemical ligation. A successful application of a cyclic peptide bearing a thioester handle in native chemical ligation is shown by a high yielding ligation.
Division of Medicinal Chemistry & Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Department of Pharmaceutical Sciences, Faculty of Sciences, Utrecht University, P.O. Box 80082, 3508 TB, Utrecht, The Netherlands.
Helmus
H
van de Langemheen
Arwin J
AJ
Brouwer
Johan
J
Kemmink
John A W
JA
Kruijtzer
Rob M J
RM
Liskamp
D003545:Cysteine / Q000031:analogs & derivatives* / Q000138:chemical synthesis*; D008026:Ligation; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000138:chemical synthesis* / Q000737:chemistry*; D013457:Sulfur Compounds / Q000138:chemical synthesis* / Q000737:chemistry*
D010456:Peptides, Cyclic; D013457:Sulfur Compounds; D003545:Cysteine