¹H, ¹³C and ¹⁵N resonance assignment of the soluble form of the lipid-modified Azurin from Neisseria gonorrhoeae.
Lipid-modified azurin (Laz) from Neisseria gonorrhoeae is a type 1 copper protein proposed to be the electron donor to several enzymes involved in the resistance mechanism to reactive oxygen and nitrogen species. Here we report the backbone and side-chain resonance assignment of Laz in the reduced form, which has been complete at 97%. The predicted secondary structure indicates that this protein belongs to the azurin subfamily of type 1 copper proteins.
REQUIMTE-CQFB, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Cláudia S
CS
Nóbrega
Manolis
M
Matzapetakis
Sofia R
SR
Pauleta
D000595:Amino Acid Sequence; D001400:Azurin / Q000737:chemistry*; D002247:Carbon Isotopes; D008055:Lipids / Q000737:chemistry*; D008969:Molecular Sequence Data; D009344:Neisseria gonorrhoeae / Q000378:metabolism*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D011522:Protons; D012995:Solubility
D002247:Carbon Isotopes; D008055:Lipids; D009587:Nitrogen Isotopes; D011522:Protons; D001400:Azurin