A simple packed bed device for antibody labelled rare cell capture from whole blood.
We have developed a system to isolate rare cells from whole blood using commercially available components and simple microfluidics. We characterized the capture of MCF-7 cells spiked into whole human blood using this system to demonstrate that enrichment and enumeration studies give results similar to in situ surface-modified devices while reducing fabrication and operation complexity.
Biochemical Science Division, NIST, 100 Bureau Dr, Gaithersburg, MD, USA.
Jason G
JG
Kralj
Chandamany
C
Arya
Alessandro
A
Tona
Thomas P
TP
Forbes
Matthew S
MS
Munson
Lynn
L
Sorbara
Sudhir
S
Srivastava
Samuel P
SP
Forry
D000906:Antibodies / Q000378:metabolism*; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*
D000906:Antibodies