Determination of presence of Tritrichomonas foetus in uterine lavages from cows with reproductive problems.
The aim of this study was to determine the presence of Tritrichomonas foetus in two dairy herds on the Altiplano Cundiboyacense. Twenty-one low-volume uterine lavages from cows with a history of reproductive problems in two dairy herds located in the municipality of Sibaté (Cundinamarca) and Ventaquemada (Boyacá) were evaluated. In the first herd, 10 cows were sampled and in the second, 11 cows, based on three inclusion criteria. The uterine lavages were obtained through infusion of physiological saline solution into the uterine body. The samples were centrifuged and seeded in Tritrichomonas basal medium for 10-15 days at 37 ºC. The protozoa were evaluated on the day of sampling and 10 and 15 days after incubation by means of direct viewing under a dark-field microscope. Positive samples were stained with Wright and Lugol to identify the morphological characteristics. This study showed that T. foetus was present in 61.8% of the animals sampled. The determination that T. foetus was present in 61.8% of the samples analyzed is a significant finding given that in the herds evaluated, this agent had not previously been diagnosed.
Unidad de Investigaciones Agropecuarias, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
Lady Carolina
LC
González-Carmona
Milena Jineth
MJ
Sánchez-Ladino
Rubiela
R
Castañeda-Salazar
Adriana Del Pilar
Adel P
Pulido-Villamarín
Humberto
H
Guáqueta-Munar
Moisés
M
Aranda-Silva
Milton Januario
MJ
Rueda-Varón
D000818:Animals; D002417:Cattle; D005260:Female; D012098:Reproduction; D007507:Therapeutic Irrigation; D016835:Tritrichomonas foetus / Q000302:isolation & purification*; D014599:Uterus / Q000469:parasitology*