Binding mechanism of patulin to heat-treated yeast cell.
OBJECTIVE
This study aims to assess the removal mechanism of patulin using heat-treated Saccharomyces cerevisiae cells and identify the role of different cell wall components in the binding process.


RESULTS
In order to understand the binding mechanism, viable cells, heat-treated cells, cell wall and intracellular extract were performed to assess their ability to remove patulin. Additionally, the effects of chemical and enzymatic treatments of yeast on the binding ability were tested. The results showed that there was no significant difference between viable (53·28%) and heat-treated yeast cells (51·71%) in patulin binding. In addition, the cell wall fraction decreased patulin by 35·05%, and the cell extract nearly failed to bind patulin. Treatments with protease E, methanol, formaldehyde, periodate or urea significantly decreased (P < 0·05) the ability of heat-treated cells to remove patulin. Fourier transform infrared (FTIR) analysis indicated that more functional groups were involved in the binding process of heat-treated cells.


CONCLUSIONS
Polysaccharides and protein are important components of yeast cell wall involved in patulin removal. In addition, hydrophobic interactions play a major role in binding processes.


CONCLUSIONS
Heat-treated S. cerevisiae cells could be used to control patulin contamination in the apple juice industry. Also, our results proof that the patulin removal process is based mainly on the adsorption not degradation.
College of Food Science and Engineering, Northwest A&F University, Yangling, China.
C
C
Guo
College of Food Science and Engineering, Northwest A&F University, Yangling, China.
Y
Y
Yuan
College of Food Science and Engineering, Northwest A&F University, Yangling, China.
T
T
Yue
College of Food Science and Engineering, Northwest A&F University, Yangling, China.
S
S
Hatab
College of Food Science and Engineering, Northwest A&F University, Yangling, China.
Z
Z
Wang
D000327:Adsorption; D001628:Beverages; D002473:Cell Wall / Q000737:chemistry*; D003666:Decontamination / Q000379:methods*; D005557:Formaldehyde / Q000737:chemistry; D006358:Hot Temperature; D027845:Malus / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D010365:Patulin / Q000737:chemistry*; D010504:Periodic Acid / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014508:Urea / Q000737:chemistry
D010504:Periodic Acid; D005557:Formaldehyde; D014508:Urea; D010365:Patulin; C009288:metaperiodate; D012697:Serine Endopeptidases; C028734:protease E; D000432:Methanol