Possible role of the WDR3 gene on genome stability in thyroid cancer patients.
The role of the WDR3 gene on genomic instability has been evaluated in a group of 115 differentiated thyroid cancer (DTC) patients. Genomic instability has been measured according to the response of peripheral blood lymphocytes to ionizing radiation (0.5 Gy). The response has been measured with the micronucleus (MN) test evaluating the frequency of binucleated cells with MN (BNMN), both before and after the irradiation. No differences between genotypes, for the BNMN frequencies previous the irradiation, were observed. Nevertheless significant decreases in DNA damage after irradiation were observed in individuals carrying the variant alleles for each of the three genotyped SNPs: rs3754127 [-8.85 (-15.01 to -2.70), P<0.01]; rs3765501 [-8.98 (-15.61 to -2.36), P<0.01]; rs4658973 [-8.70 (-14.94 to -2.46), P<0.01]. These values correspond to those obtained assuming a dominant model. This study shows for the first time that WDR3 can modulate genome stability.
Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Wilser Andrés
WA
García-Quispes
Susana
S
Pastor
Pere
P
Galofré
Josefina
J
Biarnés
Joan
J
Castell
Antonia
A
Velázquez
Ricard
R
Marcos
D018263:Adenocarcinoma, Follicular / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000483:Alleles; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary; D004249:DNA Damage; D005260:Female; D005720:Gamma Rays; D005787:Gene Frequency; D042822:Genomic Instability; D005838:Genotype; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011836:Radiation Tolerance; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology
D009687:Nuclear Proteins; C120702:WDR3 protein, human