Molecular characterization of artemisia virus A, a new sobemovirus isolated from Artemisia annua.
A distinct sobemovirus was isolated from diseased Artemisia annua plants grown in experimental culture plots in Switzerland. Electron microscopy performed on extracts of leaf and root samples of a diseased A. annua plant revealed icosahedral-30 nm viral particles. The complete nucleotide sequence of the viral genome was determined. The single positive-strand RNA of 4138 nt encodes four open reading frames with an organization similar to that described for sobemoviruses. Phylogenetic analysis revealed a close relationship to ryegrass mottle virus. The virus was efficiently acquired by healthy A. annua from contaminated soil samples. "Artemisia virus A" is tentatively proposed as a name for this new candidate member of the unassigned genus Sobemovirus.
Agroscope Changins-Wädenswil Research Station ACW, 1260, Nyon, Switzerland.
Maria-Elena
ME
Ramel
Paul
P
Gugerli
Sébastien
S
Besse
Xavier
X
Simonnet
Carole
C
Balmelli
D031172:Artemisia annua / Q000821:virology*; D016000:Cluster Analysis; D016679:Genome, Viral; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D018517:Plant Roots / Q000821:virology; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013557:Switzerland; D014771:Virion / Q000648:ultrastructure
D012367:RNA, Viral