Change in membrane fatty acid compositions and cold-induced responses in chickpea.
Plant cells often increase cold tolerance by reprogramming their genes expression which results in adjusted metabolic alternations, a process enhanced under cold acclimation (CA) phase. In present study, we assessed the changes of membrane fatty acid compositions and defense machine (like antioxidative enzymes) along with damage indexes like electrolyte leakage index (ELI) and malondialdehyde (MDA) during CA, cold stress (CS) and recovery (R) phases in chickpea (Cicer arietinum L.). Results showed an increase in unsaturated fatty acids ratio compare to saturated ones which is a sign of cold tolerance especially after CA phase. Antioxidant enzymes had an important role during CA and R phases while CS affected their activity which can be a sign for associating other metabolites or enzymes activities to create cold tolerance in plants. To investigation of enzymes assay under experimental treatments, the expression pattern of some enzymes including superoxide dismutase (sod), catalase (cat) and lipoxygenase (lox) was studied using quantitative real time PCR. LOX activity has shown a bilateral behavior: a positive relation with membrane damage index in CA and an interesting link with double bond index (DBI) in CS indicating probably its role in secondary metabolites like jasmonic acid signaling pathway. It was suggested that increased DBI and low LOX activity under CS could be a reason for plant cold tolerance.
Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Seyyede Sanam
SS
Kazemi Shahandashti
Reza
R
Maali Amiri
Hassan
H
Zeinali
Seyyede Sanaz
SS
Ramezanpour
D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D002463:Cell Membrane Permeability; D029863:Cicer / Q000166:cytology / Q000502:physiology*; D058639:Cold-Shock Response; D005227:Fatty Acids / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000166:cytology / Q000502:physiology*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
D005227:Fatty Acids; D010940:Plant Proteins; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D008084:Lipoxygenase; D013482:Superoxide Dismutase