Striatal extracts promote the dopaminergic differentiation of GFP-bone mesenchymal stem cells.
Bone mesenchymal stem cells (BMSCs) are an attractive donor graft source because of the potential of self-renewal and multi-direction differentiation. However, it is a great challenge to induce BMSCs to specifically differentiate to dopamine (DA) neurons for the treatment of Parkinson's disease. Because the striatum is the target tissue for the projection of DA neurons in the midbrain, we investigated whether its extracts could promote the dopaminergic differentiation of BMSCs. BMSCs were isolated from green fluorescent protein (GFP) transgenic mice. Flow cytometry was used to identify the expression of CD29 and CD11b in cultured BMSCs; and immunochemical staining was employed to determine the differentiation of BMSCs. Our results showed that striatal extracts could induce differentiation of BMSCs into both neurons and glia, especially the DA neurons. When transplanted to the rat striatum, GFP-BMSCs could differentiate into tyrosine hydroxylase positive neurons and demonstrate potential migration in the brain. Taking together, our results suggest that striatal extracts can specifically promote the dopaminergic differentiation of GFP-BMSCs, thereby providing a feasible strategy for the treatment of Parkinson's disease.
Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Anatomy, Shandong University School of Medicine, Jinan, Shandong 250012, China.
Shuhua
S
Yang
Qing
Q
Gao
Lihua
L
Bao
Jing
J
Zhang
Yanlai
Y
Hu
Lujun
L
Bing
Jinlong
J
Sun
Jing
J
Hao
Chao
C
Chen
Shangzhi
S
Li
W S
WS
Poon
Jinhao
J
Sun
Yingmao
Y
Gao
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000737:chemistry*; D059290:Dopaminergic Neurons / Q000166:cytology* / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D051381:Rats; D014020:Tissue Extracts / Q000737:chemistry*
D014020:Tissue Extracts; D049452:Green Fluorescent Proteins