Fast nucleic acid amplification for integration in point-of-care applications.
An ultrafast microfluidic PCR module (30 PCR cycles in 6 min) based on the oscillating fluid plug concept was developed. A robust amplification of native genomic DNA from whole blood samples could be achieved at operational conditions established from systematic investigations of key parameters including heat transfer and in particular flow velocities. Experimental data were augmented with results from computational fluid dynamics simulations. The reproducibility of the current system was substantially improved compared to previous concepts by integration of a closed reservoir instead of utilizing a vented channel end at ambient pressure rendering the devised module suitable for integration into complex sample-to-answer analysis platforms such as point-of-care applications.
Institut für Mikrotechnik Mainz GmbH, Mainz, Germany. Brunklaus@imm-mainz.de
Sabine
S
Brunklaus
Thomas E
TE
Hansen-Hagge
Julia
J
Erwes
Julian
J
Höth
Mathieu
M
Jung
Daniel
D
Latta
Xenia
X
Strobach
Christian
C
Winkler
Marion
M
Ritzi-Lehnert
Klaus S
KS
Drese
D000199:Actins / Q000235:genetics; D003198:Computer Simulation; D004247:DNA / Q000097:blood / Q000737:chemistry; D004867:Equipment Design; D006801:Humans; D008297:Male; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D015203:Reproducibility of Results; D013696:Temperature
D000199:Actins; D004247:DNA