Activities of enzymes that hydrolyze adenine nucleotides in lymphocytes from patients with rheumatoid arthritis.
The purpose of this study was to investigate the activities of ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5; CD39) and adenosine deaminase (E-ADA; EC 3.5.4.4) in lymphocytes from patients with rheumatoid arthritis (RA). Thirty patients diagnosed with RA through American College of Rheumatology criteria as well as 30 healthy patients were selected. Peripheral blood lymphocytes were isolated, and E-NTPDase and E-ADA activities were assayed. The results demonstrated an increased E-NTPDase activity (both ATP and ADP as substrates) and a decreased E-ADA activity in RA patients. These data suggest an organic effort to preserve the adenosine level, which is known to have anti-inflammatory and analgesic properties, working as a potent suppressor of immune response.
Centro de Ciências Naturais e Exatas, Departamento de Química, Universidade Federal de Santa Maria, RS, Brazil.
Jeandre Augusto
JA
Dos Santos Jaques
Lara Vargas
LV
Becker
Viviane do Carmo Gonçalves
Vdo C
Souza
Cláudio Alberto Martins
CA
Leal
Tatiana Montagner Dalcin
TM
Bertoldo
Kelly
K
de Vargas Pinheiro
Vera Maria
VM
Morsch
Maria Rosa Chitolina
MR
Schetinger
Daniela Bitencourt Rosa
DB
Leal
D000243:Adenosine Deaminase / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D001081:Apyrase / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000201:enzymology* / Q000473:pathology; D016022:Case-Control Studies; D002478:Cells, Cultured; D057075:Enzyme Assays; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008875:Middle Aged
D015703:Antigens, CD; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000243:Adenosine Deaminase; D001081:Apyrase; C067869:CD39 antigen