Towards the simplification of protein synthesis: iterative solid-supported ligations with concomitant purifications.
Please release me: a new linker for the temporary tagging of peptides at their N-terminus after solid-phase elongation, and its potential for capture/release purification is demonstrated. This concept is extended to a remarkably efficient self-purifying N-to-C iterative triazole ligation strategy, which is applied to the synthesis of a polypeptide having 160 residues, in a high purity without the need for chromatographic purification (orange blocks: peptide segments).
Centre de Biophysique Moléculaire, CNRS UPR, Orléans, France. aucagne@cnrs-orleans.fr
Vincent
V
Aucagne
Ibai E
IE
Valverde
Philippe
P
Marceau
Mathieu
M
Galibert
Nabil
N
Dendane
Agnès F
AF
Delmas
D000595:Amino Acid Sequence; D057930:Click Chemistry / Q000379:methods; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*
D010455:Peptides; D011506:Proteins