Aberrant histone acetylation and methylation levels in woman with endometriosis.
OBJECTIVE
To investigate the alterations in histone modifications in woman with endometriosis.


METHODS
Global histone H3/H4 acetylation and H3K4/H3K9 methylation in eutopic and ectopic endometrium from 15 endometriosis patients were assayed using the EpiQuik global histone H3/H4 acetylation and H3K4/H3K9 methylation assay kits. Quantitative real-time reverse transcriptase-polymerase chain reaction was applied to measure mRNA levels of 12 members of histone-related chromatin modifier genes.


RESULTS
Histone H4 hypoacetylation was detected both in eutopic and ectopic endometrium. There were no difference between patients with endometriosis and controls on global levels of H3 acetylation. Furthermore, global histone H3K4 hypomethylation and H3K9 hypomethylation were detected both in ectopic and eutopic endometrium (p < 0.001), and in ectopic endometrium (p < 0.001), respectively. SIRT1 mRNA level was significantly decreased in eutopic endometrium, while mRNA levels of HDAC1, SUV39H1, SUV39H2 and G9a were significantly downregulated in ectopic endometrium. HDAC2 mRNA level was significantly increased in eutopic endometrium. PCAF mRNA level was significantly increased in ectopic endometrium.


CONCLUSIONS
Aberrant histone modification may play an important role in the pathogenesis of endometriosis.
Department of Obstetrics and Gynecology, Second Xiangya Hospital, Central South University, #139 Renmin Middle Rd, Changsha 410011, Hunan, People's Republic of China.
Xia
X
Xiaomeng
Zhao
Z
Ming
Ma
M
Jiezhi
Fang
F
Xiaoling
D000107:Acetylation; D000328:Adult; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism; D004717:Endometrium / Q000378:metabolism; D044127:Epigenesis, Genetic; D005260:Female; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism*; D055815:Young Adult
D006657:Histones; D012333:RNA, Messenger; D011495:Histone-Lysine N-Methyltransferase; D051548:Histone Acetyltransferases; D006655:Histone Deacetylases