Expression of the murine Nr4a1 gene is controlled by three distinct genomic loci.
The transcription factor NR4A1 belongs to the class of orphan nuclear receptors without known ligand which might control its activity. Here we examined its transcriptional regulation in response to elevated cAMP levels in HL-1 cardiac myocytes and in the heart in vivo. We report, that murine Nr4a1 is expressed from distinct promoters located upto -11.5 kb from the first coding exon. Whereas in HL-1 cells only two of the three distinct transcripts were induced by cAMP, in the heart all transcripts were regulated in response to β-adrenergic stimulation.
Institut für Herz- und Kreislaufphysiologie, Heinrich-Heine-Universität, Universitätsklinikum Düsseldorf, D-40225 Düsseldorf, Germany. torben.soeker@uni-duesseldorf.de
Torben
T
Söker
Axel
A
Gödecke
D000818:Animals; D002460:Cell Line; D000242:Cyclic AMP / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology*; D056426:Genetic Loci / Q000502:physiology*; D051379:Mice; D009124:Muscle Proteins / Q000096:biosynthesis*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1 / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology
D009124:Muscle Proteins; C539818:Nr4a1 protein, mouse; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D000242:Cyclic AMP