Protective effects of sulfated chitooligosaccharides with different degrees of substitution in MIN6 cells.
Chitosan, a naturally occurring biopolymer, has received considerable attention for pharmaceutical applications due to its biocompatible, biodegradable and less toxic properties. In this study, sulfated chitooligosaccharides (COS-S) with different degrees of substitution (DS) were prepared to improve both its water-solubility and bioactivity. Additionally, the protective effects of COS-S on hydrogen peroxide (H(2)O(2))-induced dysfunction in MIN6 cells were investigated. COS-SI (DS, 0.8) and COS-SII (DS, 1.9) significantly increased the cell viability in a dose-dependent manner. COS-S also significantly suppressed NO production, the activity and mRNA expression of iNOS, and the protein level of NF-κB protein p65, which were activated by H(2)O(2). Furthermore, we demonstrated that COS-S led to an increase in the Bcl-2/Bax mRNA expression ratio and the inhibition of Caspase-3 mRNA expression in H(2)O(2)-stimulated MIN6 cells. Additionally, COS-SII (DS, 1.9) exhibited a superior protective effect on H(2)O(2)-induced apoptosis compared to COS-SI (DS, 0.8). These results indicated the good anti-oxidative capacity of COS-S and the possible mechanism via the blockade of the NF-κB signaling pathway. The protective effects of COS-S against oxidative injuries in MIN6 cells were depended on both DS and concentration.
Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China.
Xiaoyun
X
Lu
Hui
H
Guo
Lijun
L
Sun
Liyu
L
Zhang
Yali
Y
Zhang
D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000096:biosynthesis; D048271:Chitosan; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology; D013463:Sulfuric Acid Esters / Q000737:chemistry / Q000494:pharmacology; D051996:Transcription Factor RelA / Q000096:biosynthesis
D000975:Antioxidants; D009844:Oligosaccharides; D016877:Oxidants; C498143:RELA protein, human; D013463:Sulfuric Acid Esters; D051996:Transcription Factor RelA; D048271:Chitosan; D006861:Hydrogen Peroxide; C496319:NOS2 protein, human; D052247:Nitric Oxide Synthase Type II; C505407:CASP3 protein, human; D053148:Caspase 3