Novel interaction of two clock-relevant RNA-binding proteins C3 and XRN1 in Chlamydomonas reinhardtii.
The RNA-binding protein CHLAMY1 of the green alga Chlamydomonas reinhardtii consists of two subunits, named C1 and C3 that maintain the period and phase of the circadian clock. Here, we investigated if any of its subunits interact with other clock components involved in RNA metabolism. We found that C3, but not C1 strongly interacts with exoribonuclease XRN1 whose knockout results in low amplitude rhythms. XRN1 is subject to degradation by the proteasome pathway. Its level increases in cells grown at lower ambient temperature simulating night, which was also observed for C3. Our data indicate a network of clock-relevant RNA-binding proteins.
Institute of General Botany and Plant Physiology, Friedrich Schiller University, Jena, Germany.
Hannes
H
Dathe
Katja
K
Prager
Maria
M
Mittag
D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D016825:Chlamydomonas reinhardtii / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D057906:Circadian Clocks / Q000235:genetics; D002940:Circadian Rhythm / Q000235:genetics; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D005092:Exonucleases / Q000235:genetics / Q000378:metabolism*; D055786:Gene Knockout Techniques; D015151:Immunoblotting; D007976:Leupeptins / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011485:Protein Binding; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D059748:Proteolysis; D012313:RNA / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013696:Temperature; D020798:Two-Hybrid System Techniques
D020418:Algal Proteins; D015853:Cysteine Proteinase Inhibitors; D007976:Leupeptins; D021122:Protein Subunits; D016601:RNA-Binding Proteins; D012313:RNA; D005092:Exonucleases; D046988:Proteasome Endopeptidase Complex; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde