Normalizing effect of SJSZ glycoprotein (38 kDa) on proliferating cell nuclear antigen and interferon-γ in diethylnitrosamine-induced mice splenocytes.
One of the immunosuppressive responses when hepatocellular carcinoma (HCC) develops in mammals is defective proliferation in the spleen. The objective of this study was to investigate the protective effect of the Styrax japonica Siebold et al. Zuccarini (SJSZ) glycoprotein on the proliferation of splenocytes induced by diethlynitrosamine (DEN). To assess whether the SJSZ glycoprotein modulates splenocyte proliferation, Balb/c mice were injected intraperitoneally with DEN (50 mg/kg, BW) for 7 weeks. After 7 weeks, the mice were sacrificed, and spleens were isolated. We evaluated [(3) H]-thymidine incorporation, extracellular signal-regulated kinase (ERK), cell cycle-related factors [p53, p21, p27, cyclin D1/cyclin dependent kinase (CDK) 4], proliferating cell nuclear antigen and interferon (IFN)-γ using radiation activity, immunoblot analysis, and the reverse transcription-polymerase chain reaction. The results revealed that the SJSZ glycoprotein (10 mg/kg, BW) increased [(3) H]-thymidine incorporation, ERK phosphorylation, expression levels of cyclin D1/cyclin dependent kinase 4, and IFN-γ. However, the SJSZ glycoprotein decreased levels of p53, p21, and p27. Taken together, these results suggest that the SJSZ glycoprotein inhibited defective splenocyte proliferation induced by DEN.
Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, 300 Yongbong-Dong, Gwang-ju 500-757, South Korea.
Jin
J
Lee
Kye-Taek
KT
Lim
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D004052:Diethylnitrosamine / Q000008:administration & dosage / Q000009:adverse effects*; D005638:Fruit / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000494:pharmacology*; D007371:Interferon-gamma / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010766:Phosphorylation; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology; D061251:Primary Cell Culture; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000473:pathology; D032362:Styrax / Q000737:chemistry*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
C530105:Ccnd1 protein, mouse; C493961:Cdkn1b protein, mouse; D006023:Glycoproteins; D010940:Plant Proteins; D018809:Proliferating Cell Nuclear Antigen; D019938:Cyclin D1; D050760:Cyclin-Dependent Kinase Inhibitor p27; D004052:Diethylnitrosamine; D007371:Interferon-gamma; C495902:Cdk4 protein, mouse; D051358:Cyclin-Dependent Kinase 4; D013936:Thymidine