Fluorescence localization microscopy: The transition from concept to biological research tool.
Localization microscopy techniques are super-resolution fluorescence imaging methods based on the detection of individual molecules. Despite the relative simplicity of the microscope setups and the availability of commercial instruments, localization microscopy faces unique challenges. While achieving super-resolution is now routine, issues concerning data analysis and interpretation mean that revealing novel biological insights is not. Here, we outline why data analysis and the design of robust test samples may hold the key to harness the full potential of localization microscopy.
Centre for Vascular Research and the Australian Centre for NanoMedicine; University of New South Wales; Sydney, NSW Australia.
Dylan M
DM
Owen
Markus
M
Sauer
Katharina
K
Gaus