Enantioselective effects of methamidophos on the coelomocytes lysosomal membrane stability of Eisenia fetida.
Many of organophosphorous insecticides are chiral compounds. In this study, the enantioselective effects of organophosphate insecticide methamidophos on the coelomocytes lysosomal membrane stability of earthworm Eisenia fetida were studied: (1) The enantiomers of methamidophos were absolutely separated by high-performance liquid chromatography with a commercial chiral column; (2) The neutral red retention assay was used to judge the lysosomal membrane stability. The results showed that with the concentration increasing, lysosomal membranes have been significantly destroyed by individual stereoisomers and racemate of methamidophos. The neutral red retention times were significantly descended from 76.88 to 29.78 min. Both (+)- and (-)-methamidophos showed more prone to destroy the integrity of the lysosomal membrane than the racemate. However, the different effect between stereoisomers is slight.
Research Center of Environmental Science, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
Linhua
L
Chen
Xianting
X
Lu
Yun
Y
Ma
D000818:Animals; D007306:Insecticides / Q000737:chemistry / Q000633:toxicity*; D007425:Intracellular Membranes / Q000187:drug effects*; D008247:Lysosomes / Q000187:drug effects*; D009835:Oligochaeta; D009946:Organothiophosphorus Compounds / Q000737:chemistry / Q000633:toxicity*; D012989:Soil Pollutants / Q000737:chemistry / Q000633:toxicity*; D013237:Stereoisomerism
D007306:Insecticides; D009946:Organothiophosphorus Compounds; D012989:Soil Pollutants; C014655:methamidophos