Screening of lactic acid bacteria for reducing power using a tetrazolium salt reduction method on milk agar.
Reducing activity is a physiological property of lactic acid bacteria (LAB) of technological importance. We developed a solid medium with tetrazolium dyes enabling weakly and strongly reducing LAB to be discriminated. It was used to quantify populations in a mixed culture (spreading method) and screen strains (spot method).
UMR A 02.102 Unité Procédés Alimentaires et Microbiologiques, AgroSup Dijon-Université de Bourgogne, 1-esplanade Erasme, F-21000 Dijon, France.
Damien
D
Michelon
Sybille
S
Tachon
Bruno
B
Ebel
Joëlle
J
De Coninck
Gilles
G
Feron
Patrick
P
Gervais
Mireille
M
Yvon
Rémy
R
Cachon
D000362:Agar / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism*; D007777:Lactobacillaceae / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D008892:Milk / Q000378:metabolism* / Q000382:microbiology*; D010084:Oxidation-Reduction; D013778:Tetrazolium Salts / Q000737:chemistry
D003470:Culture Media; D013778:Tetrazolium Salts; D019344:Lactic Acid; D000362:Agar