Protein immobilization onto poly(acrylic acid) functional macroporous polyHIPE obtained by surface-initiated ARGET ATRP.
Amino-functional macroporous monoliths from polymerized high internal phase emulsion (polyHIPE) were surface modified with initiators for atom transfer radical polymerization (ATRP). The ATRP initiator groups on the polyHIPE surface were successfully used to initiate activator regeneration by electron transfer (ARGET) ATRP of (meth)acrylic monomers, such as methyl methacrylate (MMA) or tert-butyl acrylate (tBA) resulting in a dense coating of polymers on the polyHIPE surface. Addition of sacrificial initiator permitted control of the amount of polymer grafted onto the monolith surface. Subsequent removal of the tert-butyl protecting groups yielded highly functional polyHIPE-g-poly(acrylic acid). The versatility to use the high density of carboxylic acid groups for secondary reactions was demonstrated by the successful conjugation of enhanced green fluorescent protein (eGFP) and coral derived red fluorescent protein (DsRed) using EDC/sulfo-NHS chemistry, on the polymer 3D-scaffold surface. The materials and methodologies presented here are simple and robust, thus, opening new possibilities for the bioconjugation of highly porous polyHIPE for bioseparation applications.
School of Chemical Sciences, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin 9, Ireland.
Fabrice
F
Audouin
Ruth
R
Larragy
Mary
M
Fox
Brendan
B
O'Connor
Andreas
A
Heise
D000179:Acrylates / Q000737:chemistry; D000180:Acrylic Resins / Q000737:chemistry*; D002264:Carboxylic Acids; D049452:Green Fluorescent Proteins / Q000737:chemistry; D055767:Immobilized Proteins; D008164:Luminescent Proteins / Q000737:chemistry; D020366:Methylmethacrylate / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D013343:Styrenes / Q000737:chemistry*; D013499:Surface Properties; D000097573:Red Fluorescent Protein
D000179:Acrylates; D000180:Acrylic Resins; D002264:Carboxylic Acids; D049452:Green Fluorescent Proteins; D055767:Immobilized Proteins; D008164:Luminescent Proteins; D020366:Methylmethacrylate; D011108:Polymers; D013343:Styrenes; D000097573:Red Fluorescent Protein; C485184:enhanced green fluorescent protein; C493734:polyHIPE; C006903:carbopol 940; C522411:tert-butyl acrylate