Effects of ion beams pretreatment on damage of UV-B radiation on seedlings of winter wheat (Triticum aestivum L.).
The seeds of winter wheat were pretreated with three different doses of low-energy N(+) beams, and its seedlings were subjected to UV-B irradiation (10.08 kJ m(-2) day(-1)) at three-leaves stage. The growth characteristic of seeds, the oxidative damage to membrane system induced by UV-B radiation, and the alleviating effects of N(+) beams pretreatment to radiation damage were investigated. The results showed that the germination rate and seedling rate, respectively, increased 14.09 ± 1.03 and 13.91 ± 1.21 % compared with control (CK) at the dose of 4.0 × 10(16) ions/cm(2). When seedlings were exposed to UV-B radiation, the pretreatment method under the dose of 4.0 × 10(16) ions/cm(2) made the activity of peroxidase and superoxide dismutase increasing, the content of chlorophyll enhancing, but the content of malondialdehyde reducing significantly compared with that of the single UV-B radiation. Whereas, the activity of catalase irradiated by UV-B improved notably under the pretreatment dose of 8.0 × 10(16) ions/cm(2). In addition, after being irradiated with UV-B, the content of soluble protein and glutathione whose seeds were pretreated by the dose of 6.0 × 10(16) ions/cm(2) were higher than that of the single UV-B radiation. It was suggested that the suitable dose of low-energy ion beams pretreatment to wheat seeds could change its physiological characteristics at seedlings stage to alleviate the damage effects from UV-B radiation.
Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou, PR China. zhaoshuaipeng@163.com
Shuaipeng
S
Zhao
Qunce
Q
Huang
Pengming
P
Yang
Jiajia
J
Zhang
Hongru
H
Jia
Zhen
Z
Jiao
D000975:Antioxidants / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism; D036226:Seedlings / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D012964:Sodium / Q000737:chemistry; D012995:Solubility; D014908:Triticum / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000975:Antioxidants; D010940:Plant Proteins; D008315:Malondialdehyde; D012964:Sodium; D005978:Glutathione