Ingenane-type diterpenes with a modulatory effect on IFN-γ production from the roots of Euphorbia kansui.
A new ingenane-type diterpene, (3S,5R)5-O-(2,3-dimethylbutanoyl)-13-O-dodecanoyl-20-O-deoxyingenol (1), and six known compounds,3-O-(2,3-dimethylbutanoyl)-13-O-dodecanoyl-20-O-deoxyingenol (2), 20-O-decanoylingenol (3), 20-O-acetylingenol-3-O-(2'E,4'Z) decadienoate (4), kansuiphorin A (5), 3-O-(2,3-dimethylbutanoyl)-13-O-dodecanoylingenol (6), and kansuinin F (7) were isolated and evaluated for their effect on IFN-γ production in NK92 cells. Interestingly, subjection to compounds 4 and 6 (10 nM) displayed the most significant response in IFN-γ production, comparable to that produced by the same dose of phorbol 12-myistate 13-acetate (PMA). High doses of compounds 3 (100 nM), 1 (1. 25 μM) and 5 (5.0 μM) have also been shown to activate the IFN-γ production.
Natural Medicine Research Center, KRIBB, ChungBuk, 363-883, Korea.
Piseth
P
Khiev
Jae Wha
JW
Kim
Song Jae
SJ
Sung
Hyuk-Hwan
HH
Song
Dong-Ho
DH
Choung
Young-Won
YW
Chin
Hyeong-Kyu
HK
Lee
Sei-Ryang
SR
Oh
D018926:Anti-Allergic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D055808:Drug Discovery; D028481:Euphorbia / Q000737:chemistry*; D006801:Humans; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007371:Interferon-gamma / Q000378:metabolism*; D059425:Interferon-gamma Release Tests; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D055670:Medicine, Korean Traditional; D009994:Osmolar Concentration; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D056910:Republic of Korea; D013329:Structure-Activity Relationship
D018926:Anti-Allergic Agents; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; C573415:IFNG protein, human; D007155:Immunologic Factors; D010936:Plant Extracts; C530110:ingenane; D007371:Interferon-gamma