Genotype and haplotype analysis of the AZGP1 gene in cattle.
Zinc-a2-glycoprotien (AZGP1) involved in lipid metabolism and associated with adipose tissue atrophy in cachexia. And it also related to sperm motility and in turn fertilization. To ascertain whether there were mutations in the bovine AZGP1 gene, this study investigated variation of the AZGP1 gene through PCR-SSCP and sequencing. Four missense mutations were identified in 649 cattle from six independent populations. Haplotype frequencies and linkage disequilibrium (LD) coefficients of these SNPs in three Chinese indigenous cattle breeds were analyzed. One LD block was found in three cattle breeds. The statistical analyses indicated that AC genotype of Z4 locus was associated with the high body weight, body length and chest girth in Jiaxian cattle breed (P < 0.05). Our results provided evidence that polymorphisms in the AZGP1 gene were associated with growth traits, and may be used for marker-assisted selection and management in cattle breeding program.
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, No. 22 Xinong Road, Yangling, 712100, Shaanxi, People's Republic of China. zhangbao1983@gmail.com
Bao
B
Zhang
Yi Kun
YK
Guo
Shengbin
S
Li
Liang Zhi
LZ
Zhang
Xian Yong
XY
Lan
Shen Rong
SR
Hu
Hong
H
Chen
D000818:Animals; D002417:Cattle / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D056726:Genetic Association Studies; D056426:Genetic Loci / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D019655:Quantitative Trait, Heritable
D006023:Glycoproteins