Experimental infection of United States swine with a Chinese highly pathogenic strain of porcine reproductive and respiratory syndrome virus.
The pathogenesis of Type 2 highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) in 10-week old swine in the United States was investigated. rJXwn06, rescued from an infectious clone of Chinese HP-PRRSV, replicated in swine with at least 100-fold increased kinetics over U.S. strain VR-2332. rJXwn06 caused significant weight loss, exacerbated disease due to bacterial sepsis and more severe histopathological lung lesions in pigs exposed to HP-PRRSV than to those infected with VR-2332. Novel findings include identification of bacterial species present, the degree of thymic atrophy seen, and the inclusion of contact animals that highlighted the ability of HP-PRRSV to rapidly transmit between animals. Furthermore, comprehensive detailed cytokine analysis of serum, bronchoalveolar lavage fluid, and tracheobronchial lymph node tissue homogenate revealed a striking elevation in levels of cytokines associated with both innate and adaptive immunity in HP-PRRSV infected swine, and showed that contact swine differed in the degree of cytokine response.
Veterinary Diagnostic & Production Animal Medicine, Iowa State University, Ames, IA, USA.
Baoqing
B
Guo
Kelly M
KM
Lager
Jamie N
JN
Henningson
Laura C
LC
Miller
Sarah N
SN
Schlink
Matthew A
MA
Kappes
Marcus E
ME
Kehrli
Susan L
SL
Brockmeier
Tracy L
TL
Nicholson
Han-Chun
HC
Yang
Kay S
KS
Faaberg
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D002681:China; D016207:Cytokines / Q000378:metabolism; D008168:Lung; D016676:Macrophages, Alveolar; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000503:physiopathology* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000472:pathogenicity*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000503:physiopathology* / Q000821:virology; D013950:Thymus Gland / Q000473:pathology; D014481:United States
D016207:Cytokines