[Effect of docetaxel on C-jun and androgen receptor interaction in prostate cancer LNCaP cells and its androgen-independence subtype LNCaP-bic cells].
OBJECTIVE
To investigate the effect of docetaxel on the interaction between C-jun and androgen receptor (AR) in prostate cancer LNCaP cells and its androgen-independence subtype LNCaP-bic cells.


METHODS
LNCaP and LNCaP-bic cells were treated with docetaxel and the changes in AR and AP-1 expression were evaluated using luciferase assay. Western blotting and immunoprecipitation assay were employed to analyze the effects of docetaxel on the expressions of C-jun and AR and their interaction.


RESULTS
Luciferase assay showed that LNCaP and LNCaP-bic cells expressed higher levels of AR and C-jun after docetaxel treatment. Docetaxel induced a higher level of p-c-jun expression in LNCaP-bic cells than in the parental LNCaP cells. Western blotting showed a strong PSA protein expression in LNCaP-bic cell after docetaxel treatment. Docetaxel caused a stronger interaction between AR and C-jun in LNCaP-bic cells.


CONCLUSIONS
Docetaxel activates ligand-independent AR transcription, and the interaction between AR and C-jun may affect the outcome of docetaxel chemotherapy.
Department of Urology, Southern Medical University, Guangzhou, China. cbshen@126.com
Binshen
B
Chen
Yawen
Y
Xu
Abai
A
Xu
Chunxiao
C
Liu
Shaobo
S
Zheng
Hulin
H
Li
Kai
K
Xu
D045744:Cell Line, Tumor; D000077143:Docetaxel; D006801:Humans; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism*; D043823:Taxoids / Q000494:pharmacology*
D016755:Proto-Oncogene Proteins c-jun; D011944:Receptors, Androgen; D043823:Taxoids; D000077143:Docetaxel