Bioactive hyaluronan fragment (hexasaccharide) detects specific hexa-binding proteins in human breast and stomach cancer: possible role in tumorogenesis.
Hyaluronan (HA) is a component of extracellular matrix that influences cell-proliferation, migration, development, regeneration, normal tissue remodeling, tissues undergoing malignancy and tumor cell interaction. The widespread occurrence of HA binding proteins, their involvement in tissue organization and the control of cellular behavior are well documented. The low molecular mass HA fragments can also induce a variety of biological events, including chemokine gene expression, transcription factor expression and angiogenesis. It is believed that these fragments are more potent in cellular activities than high molecular mass HA. In this study, we isolated the various fragments by gel permeation chromatography of hyaluronidase digested HA and characterized by fluoro assisted carbohydrate electrophoresis (FACE) and matrix assisted laser desorption ionization analysis (MALDI). Detection and distribution of cellular receptors in invasive tumor tissues for HA polymer and HA fragments were determined both by Western blot and histochemistry. The study demonstrated the overexpression of HA-hexa binding protein in human tumors of breast and stomach and its involvement in tumorogenesis.
Department of Genetics and Development, Columbia University, New York, USA.
Prashanth
P
Srinivas
Srinivas Prasad
SP
Kollapalli
Anil
A
Thomas
Karuna Kumar
KK
Mortha
Shib Das
SD
Banerjee
D001943:Breast Neoplasms / Q000473:pathology*; D006801:Humans; D018960:Hyaluronan Receptors / Q000302:isolation & purification / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D009844:Oligosaccharides / Q000302:isolation & purification / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000473:pathology*; D013379:Substrate Specificity
D018960:Hyaluronan Receptors; D009844:Oligosaccharides; D011956:Receptors, Cell Surface; C055213:saccharide-binding proteins; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase