Bench scale production of benzohydroxamic acid using acyl transfer activity of amidase from Alcaligenes sp. MTCC 10674.
The acyl transfer activity of the amidase of Alcaligenes sp. MTCC 10674 has been applied to the conversion of benzamide and hydroxylamine to benzohydroxamic acid. The unique features of the acyl transfer activity of this organism include its optimal activity at 50 °C and very high substrate (100 mM benzamide) and product (90 mM benzohydroxamic acid) tolerance among the hitherto reported enzymes. The bench scale production of benzohydroxamic acid was carried out in a fed-batch reaction (final volume 1 l) by adding 50 mM benzamide and 250 mM of hydroxylamine after every 20 min for 80 min in 0.1 M potassium phosphate buffer (pH 7.0) at 50 °C, using resting cells equal to 4.0 mg dcm/ml of reaction mixture. From 1 l of reaction mixture 33 g of benzohydroxamic acid was recovered with 24.6 g l(-1) h(-1) productivity. The acyl transfer activity of the amidase of Alcaligenes sp. MTCC 10674 and the process developed in the present study are of industrial significance for the enzyme-mediated production of benzohydroxamic acid.
Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, Himachal Pradesh 171005, India.
Ravi Kant
RK
Bhatia
Shashi Kant
SK
Bhatia
Praveen Kumar
PK
Mehta
Tek Chand
TC
Bhalla
D000421:Alcaligenes / Q000201:enzymology*; D000581:Amidohydrolases / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D006863:Hydrogen-Ion Concentration; D006877:Hydroxamic Acids / Q000378:metabolism*; D013696:Temperature
D006877:Hydroxamic Acids; D000581:Amidohydrolases; C019665:amidase; C006696:benzohydroxamic acid