Identification and function of myeloid differentiation factor 88 (MyD88) in Litopenaeus vannamei.
Myeloid differentiation factor 88 (MyD88) is a universal and essential signaling protein in Toll-like receptor/interleukin-1 receptor-induced activation of nuclear factor-kappa B. In this study, two MyD88 protein variants (LvMyD88 and LvMyD88-1) were identified in Litopenaeus vannamei. The LvMyD88 cDNA is 1,848 bp in length and contains an open reading frame (ORF) of 1,428 bp, whereas the LvMyD88-1 cDNA is 1,719 bp in length and has an ORF of 1,299 bp. Both variants encode proteins with death and Toll/interleukin-1 receptor domains and share 91% sequence identity. In healthy L. vannamei, the LvMyD88 genes were highly expressed in hemocytes but at a low level in the hepatopancreas. The LvMyD88s expression was induced in hemocytes after challenge with lipopolysaccharide, CpG-ODN2006, Vibrio parahaemolyticus, Staphyloccocus aureus, and white spot syndrome virus, but not by poly I∶C. Overexpression of LvMyD88 and LvMyD88-1 in Drosophila Schneider 2 cells led to activation of antimicrobial peptide genes and wsv069 (ie1), wsv303, and wsv371. These results suggested that LvMyD88 may play a role in antibacterial and antiviral response in L. vannamei. To our knowledge, this is the first report on MyD88 in shrimp and a variant of MyD88 gene in invertebrates.
MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.
Shuang
S
Zhang
Chao-Zheng
CZ
Li
Hui
H
Yan
Wei
W
Qiu
Yong-Gui
YG
Chen
Pei-Hui
PH
Wang
Shao-Ping
SP
Weng
Jian-Guo
JG
He
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004330:Drosophila / Q000166:cytology / Q000235:genetics; D005786:Gene Expression Regulation; D006434:Hemocytes / Q000187:drug effects / Q000502:physiology / Q000821:virology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics* / Q000378:metabolism*; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology / Q000821:virology; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013211:Staphylococcus aureus / Q000472:pathogenicity; D014736:Vibrio parahaemolyticus / Q000472:pathogenicity; D046848:White spot syndrome virus 1 / Q000472:pathogenicity
D023181:Antimicrobial Cationic Peptides; D008070:Lipopolysaccharides; D053594:Myeloid Differentiation Factor 88