Detecting motifs and patterns at mobile genetic element insertion site.
Mobile genetic elements (MGEs) occupy major proportion of eukaryotic genomes and are present in significant numbers in prokaryote genomes also. Here we report a new method which extracts a motif at the site of insertion of MGE using tools such as DNA SCANNER. The flanking region of the insertion site is extracted and is analyzed in DNA Scanner for physiochemical properties like protein-interaction measures, energy profiles as well as structural parameters. In case significant signals are observed, the most frequently occurring n-mer (5<n<20) is identified. We observed signals between 9-12 base pairs (bps) upstream in pre-insertion site of Alu element in Human and most frequently occurring motif is found to be TTAAAA. The similar signals and motif is observed at insertion site of B1 element. In lower eukaryotes such as E. histolytica, signals for EhSine1 are found at around 5 bps upstream of insertion and most frequently occurring motif is found to be AAGGT and TCGAA. Signals for Ty3 element in S. cerevisiae are found at 0-3 bps upstream of tRNA, and most frequent motif is GTTCGA (6 bps), GGTTCGA (7 bps) and GGTTCGAT (8 bps). P-element of Drosophila showed remarkable dyad peaks suggesting palindromic site of insertion.
Department of Biotechnology, Jaypee Institute of Information Technology, Noida 201307, UP, India ; Infosys Ltd, Bangalore, Karnataka, India.
Bhuvan Bhaskar
BB
Dev
Aman
A
Malik
Kamal
K
Rawal