Expression profile analysis of genes involved in horizontal gravitropism bending growth in the creeping shoots of ground-cover chrysanthemum by suppression subtractive hybridization.
The molecular mechanisms underlying gravitropic bending of shoots are poorly understood and how genes related with this growing progress is still unclear. To identify genes related to asymmetric growth in the creeping shoots of chrysanthemum, suppression subtractive hybridization was used to visualize differential gene expression in the upper and lower halves of creeping shoots of ground-cover chrysanthemum under gravistimulation. Sequencing of 43 selected clones produced 41 unigenes (40 singletons and 1 unigenes), which were classifiable into 9 functional categories. A notable frequency of genes involve in cell wall biosynthesis up-regulated during gravistimulation in the upper side or lower side were found, such as beta tubulin (TUB), subtilisin-like protease (SBT), Glutathione S-transferase (GST), and expensing-like protein (EXP), lipid transfer proteins (LTPs), glycine-rich protein (GRP) and membrane proteins. Our findings also highlighted the function of some metal transporter during asymmetric growth, including the boron transporter (BT) and ZIP transporter (ZT), which were thought primarily for maintaining the integrity of cell walls and played important roles in cellulose biosynthesis. CmTUB (beta tubulin) was cloned, and the expression profile and phylogeny was examined, because the cytoskeleton of plant cells involved in the plant gravitropic bending growth is well known.
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Shengjun
S
Xia
Yu
Y
Chen
Jiafu
J
Jiang
Sumei
S
Chen
Zhiyong
Z
Guan
Weimin
W
Fang
Fadi
F
Chen
D000595:Amino Acid Sequence; D031202:Chrysanthemum / Q000145:classification / Q000235:genetics* / Q000254:growth & development*; D018076:DNA, Complementary; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D018522:Gravitropism / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics; D018520:Plant Shoots / Q000235:genetics* / Q000254:growth & development*; D016415:Sequence Alignment
D018076:DNA, Complementary; D010940:Plant Proteins