The proteome of copper, iron, zinc, and manganese micronutrient deficiency in Chlamydomonas reinhardtii.
Trace metals such as copper, iron, zinc, and manganese play important roles in several biochemical processes, including respiration and photosynthesis. Using a label-free, quantitative proteomics strategy (MS(E)), we examined the effect of deficiencies in these micronutrients on the soluble proteome of Chlamydomonas reinhardtii. We quantified >10(3) proteins with abundances within a dynamic range of 3 to 4 orders of magnitude and demonstrated statistically significant changes in ~200 proteins in each metal-deficient growth condition relative to nutrient-replete media. Through analysis of Pearson's coefficient, we also examined the correlation between protein abundance and transcript abundance (as determined via RNA-Seq analysis) and found moderate correlations under all nutritional states. Interestingly, in a subset of transcripts known to significantly change in abundance in metal-replete and metal-deficient conditions, the correlation to protein abundance is much stronger. Examples of new discoveries highlighted in this work include the accumulation of O(2) labile, anaerobiosis-related enzymes (Hyd1, Pfr1, and Hcp2) in copper-deficient cells; co-variation of Cgl78/Ycf54 and coprogen oxidase; the loss of various stromal and lumenal photosynthesis-related proteins, including plastocyanin, in iron-limited cells; a large accumulation (from undetectable amounts to over 1,000 zmol/cell) of two COG0523 domain-containing proteins in zinc-deficient cells; and the preservation of photosynthesis proteins in manganese-deficient cells despite known losses in photosynthetic function in this condition.
Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, USA.
Scott I
SI
Hsieh
Madeli
M
Castruita
Davin
D
Malasarn
Eugen
E
Urzica
Jonathan
J
Erde
M Dudley
MD
Page
Hiroaki
H
Yamasaki
David
D
Casero
Matteo
M
Pellegrini
Sabeeha S
SS
Merchant
Joseph A
JA
Loo
D016825:Chlamydomonas reinhardtii / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D003300:Copper / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007501:Iron / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D018977:Micronutrients / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D010788:Photosynthesis; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D010970:Plastocyanin / Q000378:metabolism; D020543:Proteome; D012313:RNA / Q000032:analysis; D015032:Zinc / Q000378:metabolism
D018977:Micronutrients; D010940:Plant Proteins; D020543:Proteome; D008345:Manganese; D012313:RNA; D003300:Copper; D010970:Plastocyanin; D007501:Iron; D010088:Oxidoreductases; C029746:uptake hydrogenase; D015032:Zinc