[Effect of lycopene on the apoptosis of human peripheral endothelial progenitor cells cultivated in high concentration of glucose].
OBJECTIVE
To investigate the effect of lycopene treatment on the proliferation and apoptosis of endothelial progenitor cells (EPCs) incubated in a culture medium with high concentration of glucose.


METHODS
Mononuclear cells (MNCs) were isolated from human peripheral blood by Ficoll density gradient centrifugation. After being induced to differentiation, the endothelial progenitor cells (EPC) were identified by FITC-labeled Ulex europaeus agglutinin I and Dil labeled acetylated low density lipoprotein dual stain method. Then MTT assay and flow cytometry were used to assess the proliferation and apoptosis of EPCs.


RESULTS
The glucose in a concentration of 33 mmol/L significantly inhibited the proliferation and promoted the apoptosis of EPCs (P < 0.05). The proliferation of EPCs in 10, 30 and 50 microg/ml lycopene groups were significantly higher than the 0 microg/ml group. The rate of apoptosis were significantly lower than the lycopene 0 microg/ml group (P < 0.05).


CONCLUSIONS
High concentration of glucose attenuates the proliferative activity and increases the apoptotic rate of EPCs. Lycopene promotes the proliferation and reduces the apoptosis of EPCs cultivated in high glucose medium.
Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China. 0731zyc@163.com
Yaochi
Y
Zeng
Gulping
G
Mu
Shufen
S
Huang
Xuehui
X
Zeng
Shunmin
S
Li
D017209:Apoptosis; D002338:Carotenoids / Q000494:pharmacology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D008077:Lipoproteins, LDL; D000077276:Lycopene; D013234:Stem Cells
D008077:Lipoproteins, LDL; C032629:acetyl-LDL; D002338:Carotenoids; D005947:Glucose; D000077276:Lycopene