Bias in topoisomerase (TOPO)-cloning of multitemplate PCR products using locked nucleic acid (LNA)-substituted primers.
Locked nucleic acid (LNA) modifications help to improve nucleic acid recognition in molecular biology applications. We report that LNA-substituted primers in PCR reactions may cause considerable cloning bias when the widely used topoisomerase-based ligation is used for cloning of multitemplate PCR products.
University of Bremen, Department of Microbe-Plant Interactions, CBIB (Center for Biomolecular Interactions Bremen), 28359 Bremen, Germany.
Yuan
Y
Liu
Jessica
J
Döring
Thomas
T
Hurek
D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000235:genetics*; D026942:DNA Topoisomerases / Q000378:metabolism*; D009841:Oligonucleotides / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
D017931:DNA Primers; D009841:Oligonucleotides; C477371:locked nucleic acid; D026942:DNA Topoisomerases