Application of ISSR markers for verification of F₁ hybrids in mungbean (Vigna radiata).
Mungbean improvement via hybridization requires the  identification of true F(1) hybrids  from controlled crosses before further generations of selfing/crossing and  selection. We utilized inter-simple sequence repeat (ISSR) markers for  identifying putative F(1) hybrids  from six cross combinations whose morphological characteristics were very  similar to those of their respective female parents and could not be visually  discriminated from the self-pollinated progeny. Based on 10 ISSR primers,  polymorphisms were found between female and male parents of all six cross  combinations. The highest value of genetic differentiation (21.4%) was found  between male and female parents of the SUT3 x M5-1 cross. These 10 ISSR primers  gave 2.8-25.0% polymorphism between male and female parents, with a mean of  12.1%, and 0-13.0% polymorphism between F(1) hybrid and female parents, with a mean of 4.8%. F(1) hybrids of all six cross combinations could be  differentiated from the self-pollinated progeny of their female parents by  using only either ISSR 841 or 857 primers, together with the ISSR 835 primer.  We conclude that ISSR markers are useful and efficient for identifying mungbean  F(1) hybrids in controlled crosses from  different genetic background.
School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
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Khajudparn
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Prajongjai
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Poolsawat
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Tantasawat
D003433:Crosses, Genetic; D018744:DNA, Plant / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D005819:Genetic Markers; D006824:Hybridization, Genetic; D018895:Microsatellite Repeats / Q000235:genetics*; D015203:Reproducibility of Results
D018744:DNA, Plant; D005819:Genetic Markers