Bioprocess design for the microbial production of natural phenolic compounds by Debaryomyces hansenii.
Debaryomyces hansenii NRRL Y-7426 metabolised ferulic acid into different phenolic compounds using a factorial design where glucose concentration (in the range of 1-20 g/L), peptone concentration (2-20 g/L) and yeast extract concentration (0.2-10 g/L) were the independent variables. The interrelationship between dependent and operational variables was well fitted (R                 (2) > 0.95) to models including linear, interaction and quadratic terms. Depending on the glucose and nitrogen concentrations, which redirected the metabolism, the major degradation products were 1,226.2 mg 4-vinyl guaiacol/L after 72 h (molar yield of 86.0 %), 1,077.8 mg vanillic acid/L after 360 h (molar yield of 91.1 %) or 1,682.6 mg acetovanillone/L after 408 h (molar yield of 98.8 %) in fermentations carried out with 2,000 mg ferulic acid/L. Other metabolites such as vanillin, vanillyl alcohol or 4-ethylguaiacol were present in lower amounts.
Chemical Engineering Department, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004, Ourense, Spain.
Belén
B
Max
Francisco
F
Tugores
Sandra
S
Cortés-Diéguez
José M
JM
Domínguez
D000098:Acetophenones / Q000378:metabolism; D001688:Biological Products / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D001711:Biotransformation; D003373:Coumaric Acids / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006139:Guaiacol / Q000031:analogs & derivatives / Q000378:metabolism; D010461:Peptones / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*; D014641:Vanillic Acid / Q000378:metabolism
D000098:Acetophenones; D001688:Biological Products; D003373:Coumaric Acids; D010461:Peptones; D010636:Phenols; D006139:Guaiacol; C014245:4-vinylguaiacol; C004999:ferulic acid; C056165:acetovanillone; D014641:Vanillic Acid; D005947:Glucose