Direct observation of internalization and ROS generation of amyloid β-peptide in neuronal cells at subcellular resolution.
Seeing in many colors: Confocal images acquired using fluorescently labeled amyloid β-peptide revealed its efficient internalization by endocytosis into endosomes/lysosomes of human neuronal cells with a small portion reaching mitochondria, inducing marked cellular and mitochondrial reactive oxygen species production.
Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of the Ministry of Education, Shanxi University, Taiyuan 030006, P. R. China.
Yong
Y
Jiao
Yi
Y
Zhang
Yibin
Y
Wei
Zhiwei
Z
Liu
Wenting
W
An
Maolin
M
Guo
D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis / Q000138:chemical synthesis; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012235:Rhodamines / Q000737:chemistry; D014407:Tumor Cells, Cultured
D016229:Amyloid beta-Peptides; D005456:Fluorescent Dyes; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; D012235:Rhodamines; C078592:amyloid beta-protein (1-40); C022027:lissamine rhodamine B