Goose RIG-I functions in innate immunity against Newcastle disease virus infections.
Mammalian retinoic acid-inducible gene I (RIG-I) is a chief antiviral gene sensing viral RNA molecules including Newcastle disease virus (NDV). In this study, goose RIG-I gene (gRIG-I) was identified. The 2805 bp-long gene encodes a gRIG-I protein that exhibits 93.8% amino acid identity to duck RIG-I. DF-1 chicken fibroblast cells transfected with full-length of gRIG-I or CARD domain of gRIG-I plasmids respond significantly to the agonist of 21-mer 5'ppp RNA, evident through enhancement of IFN-β promoter activity. Goose RIG-I transfected 293T/17 cells were then tested for the response to NDV infection, resulting in up-regulated activity of IFN-β promoter, and mRNA levels of IRF-3 and IFIT1, but decreased virus titer. Similar results were obtained in transfected DF-1 chicken fibroblast cells and goose embryo fibroblast cells in response to NDV infections Animal experiments further support a role of gRIG-I in goose innate immunity against NDV infections by showing increased gRIG-I mRNA levels and decreased virus titer in geese lung and air sac post-infection.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
Yingjie
Y
Sun
Na
N
Ding
Siyu Serena
SS
Ding
Shengqing
S
Yu
Chunhun
C
Meng
Hongjun
H
Chen
Xusheng
X
Qiu
Shilei
S
Zhang
Yang
Y
Yu
Yuan
Y
Zhan
Chan
C
Ding
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D002645:Chickens; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000276:immunology*; D004372:Ducks / Q000276:immunology / Q000821:virology; D005777:Geese / Q000276:immunology* / Q000821:virology; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D050835:Interferon Regulatory Factors / Q000235:genetics / Q000276:immunology; D016899:Interferon-beta / Q000235:genetics / Q000276:immunology; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000276:immunology* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics*; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment; D014162:Transfection
D002352:Carrier Proteins; D050835:Interferon Regulatory Factors; D012333:RNA, Messenger; D011994:Recombinant Proteins; D016899:Interferon-beta; D053487:DEAD-box RNA Helicases