Atomic force microscopy captures folded ribosome bound nascent chains.
Direct visualization of co-translational folding of nascent polypeptide chains is challenging. Here we present, for the first time, AFM images of large protein constructs based on the membrane binding domain of ankyrin-R, complexed with the ribosome. The characteristic "horse-shoe" shape of ankyrin-R emerging from the ribosome was captured.
Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708, USA. al95@duke.edu
Anna
A
Loksztejn
Zackary
Z
Scholl
Piotr E
PE
Marszalek
D017487:Ankyrins / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force / Q000379:methods*; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D011487:Protein Conformation; D017510:Protein Folding; D017434:Protein Structure, Tertiary; D012270:Ribosomes / Q000378:metabolism*
D017487:Ankyrins; D029968:Escherichia coli Proteins; D010455:Peptides