[Determination of 2,4,6-trichloroanisole in wine by head-space solid phase micro-extraction and gas chromatography-mass spectrometry].
OBJECTIVE
To establish a method of combining gas chromatography-mass spectrometry (GC-MS) with head-space solid phase micro-extraction (HS-SPME) for the detection of 2,4,6-trichloroanisole (2,4,6-TCA) in wine.


METHODS
2,4,6-TCA in wine were extracted by polydimethylsiloxane (PDMS,100 microm) fiber for 15 min under 35 degrees C in a water bath. The fiber was then transferred into the injection port of the GC-MS and separated by a CP-SIL 8CB-MS capillary column (30m x 0.25mm x 0.25 microm), and detected by mass spectrometry using d5-2,4,6-TCA as an internal standard.


RESULTS
The linear range for 2,4,6-TCA was 5 (60ng/L, the detection limit was 0.06ng/L. The recovery of standard addition (5, 10, 20ng/L) was in the range of 79.8% (101.6% with relative standard deviations (RSD, n=5) < or = 7%.


CONCLUSIONS
Organic solvents were avoided and the selection was sensitive for the method, which is suitable for the determination of 2,4,6-TCA in wine.
Institute of Nutrition and Food Safety, China CDC, Beijing 100021, China. liuqingscn@163.com
Qing
Q
Liu
Qiding
Q
Zhong
Jingguang
J
Li
Jiangtao
J
Xing
Feifei
F
Huang
Yunfeng
Y
Zhao
Yongning
Y
Wu
D000840:Anisoles / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D057230:Limit of Detection; D052616:Solid Phase Extraction; D014920:Wine
D000840:Anisoles; C110228:2,4,6-trichloroanisole