Zinc finger protein-activating transcription factor up-regulates vascular endothelial growth factor-a expression in vitro.
OBJECTIVE
To construct the zinc finger protein-activating transcription factor (ZFP-ATF) plasmid and evaluate its efficacy in inducing vascular endothelial growth factor (VEGF) expression in EY.HY926 endothelial cells.


METHODS
Firstly, we constructed the ZFP-ATF plasmid, then testified the quantity of VEGF protein in EY.HY926 endothelial cells after transfected with ZFP-ATP plasmid by Western blot, finally, we used the RT-PCR to testify whether the ZFP-ATF can stimulate expression of VEGF splice variants.


RESULTS
The ZFP-ATF DNA sequences were located the multiclone sites of PVAX1 vector between the site of BamH1and Xhol.Western blot result showed VEGF expression in EY.HY926 endothelial cells transfected with ZFP-ATF plasmid was significantly higher than that in cells transfected with VEGF165 (19.95±3.95 vs.12.15±1.55 μg÷μL, P<0.01).RT-PCR result showed VEGF-A mRNA expression level induced by ZFP-ATF was high than that induced by VEGF165.


CONCLUSIONS
ZFP-ATF can up-regulate the VEGF-A expression in comparison with VEGF165, which might have beneficial effects in angiogenesis process.
Department of Vascular Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences &amp&nbsp; Peking Union Medical College, Beijing 100730, China.
Lian
L
Li-Shan
Yang
Y
Yao-Guo
Liu
L
Wei
Guo
G
Li-Long
Guan
G
Heng
Liu
L
Chang-Wei
Li
L
Yong-Jun
D051696:Activating Transcription Factors / Q000502:physiology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D018919:Neovascularization, Physiologic; D010957:Plasmids; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D016335:Zinc Fingers / Q000502:physiology*
D051696:Activating Transcription Factors; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A