Antitumoral effects of Allium sivasicum on breast cancer in vitro and in vivo.
This work aims to investigate the antiproliferative properties of Allium sivasicum (AS) on breast cancer. AS extracts were studied for cytotoxicity against the breast cancer cell lines. In vitro apoptosis studies of breast cancer cells were performed by annexin V staining in flow cytometry analyses. AS showed cytotoxicity to three cancer cell lines. Annexin-positive cells level in AS treated cell lines were higher than the untreated control cells. The expressions of caspase-7 protein and TUNEL positive cells were much higher for the rats treated by AS, compared with the untreated control group. The expressions of the Ki-67 decreased in treatment groups compared with the control group. In vivo studies showed that mean tumor volume inhibition ratio in AS treated group was 38 % compared with the untreated rats. These results indicate that A. sivasicum has antitumoral potential against breast cancer.
Department of Moleculer Biology and Genetics, Faculty of Science, Cumhuriyet University, 58140 Sivas, Turkey.
Bektas
B
Tepe
Ersin
E
Tuncer
Serpil Unver
SU
Saraydın
Hatice
H
Ozer
Metin
M
Sen
Kursat
K
Karadayi
Deniz Sahin
DS
Inan
Sule
S
Karadayi
Zübeyde
Z
Polat
Askin
A
Akpulat
Mustafa
M
Duman
Binnur
B
Koksal
Mustafa
M
Turan
D000490:Allium / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D061986:MCF-7 Cells; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D051381:Rats; D047368:Tumor Burden / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D053179:Caspase 7