Improved DNA extraction efficiency from low level cell numbers using a silica monolith based micro fluidic device.
The evaluation of a micro fluidic system with an integrated silica monolith for performing DNA extraction from limited biological samples has been carried out. Low DNA target concentrations usually require the addition of carrier RNA to ensure desired extraction efficiencies. Here, we demonstrate a micro fluidic extraction system with increasingly efficient extraction performances for biological samples containing <15 ng of total DNA without the need of adding carrier nucleic acids. All extracted DNA showed successful amplification via the polymerase chain reaction demonstrating both the effectiveness of the proposed system at removing potential inhibitors and yielding good quality DNA. The work presented here beneficially identifies reduced sample volumes/concentrations as suitable for processing with respect to downstream analysis by enabling pre-concentration of the biological sample, particularly important when dealing with clinical or forensic specimens.
Department of Chemistry, University of Hull, Cottingham Road, Hull HU6 7RX, UK. l.kashkary@2008.hull.ac.uk
Loay
L
Kashkary
Cordula
C
Kemp
Kirsty J
KJ
Shaw
Gillian M
GM
Greenway
Stephen J
SJ
Haswell
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D051379:Mice; D046210:Microfluidic Analytical Techniques; D016133:Polymerase Chain Reaction; D012822:Silicon Dioxide / Q000737:chemistry*
D012822:Silicon Dioxide; D004247:DNA