Larvicidal activity of lansiumamide B from the seeds of Clausena lansium against Aedes albopictus (Diptera: Culicidae).
The larvicidal activity of crude petroleum ether, toluene, n-butanol, ethyl acetate, acetone, and methanol extracts of the seeds of Clausena lansium was assayed for their toxicities against the early fourth instar larvae of Aedes albopictus. The larval mortality was observed after 24-h exposure. The LC(50) value of petroleum ether extract was 22.99 ppm, showing the best larvicidal activity among all six solvent extracts. A cinnamon amide compound lansiumamide B (N-methyl-N-cis-styrylcinnamamide) was isolated from the petroleum ether extract by column chromatographic method, which exhibited a strong larvicidal activity against the early fourth instar larvae of A. albopictus with LC(50) and LC(90) values of 0.45 and 2.19 ppm, respectively. The structure was elucidated by (1)H NMR, (13)C NMR spectral data. The larvicidal activity against mosquito of lansiumamide B from the seed of C. lansium was evaluated for the first time.
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Wushan, Guangzhou 510642, People's Republic of China.
Yun
Y
Han
Li-chun
LC
Li
Wen-bo
WB
Hao
Ming
M
Tang
Shu-qing
SQ
Wan
D000330:Aedes / Q000187:drug effects*; D000818:Animals; D002853:Chromatography, Liquid; D002934:Cinnamates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D054700:Clausena / Q000737:chemistry*; D007306:Insecticides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D013343:Styrenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016019:Survival Analysis
D002934:Cinnamates; D007306:Insecticides; C000593604:N-methyl-N-styrylcinnamamide; D010936:Plant Extracts; D013343:Styrenes