Patterned silk film scaffolds for aligned lamellar bone tissue engineering.
The alignment and osteogenic differentiation of MSCs on patterned silk films (PF) is investigated as a bottom-up approach toward engineering bone lamellae. Screening PF with various groove dimensions shows that cell alignment is mediated by both the pattern width and depth. MSCs are differentiated in osteogenic medium for four weeks on flat films and on the PF that produce the best alignment. The PF support osteogenic differentiation while also inducing lamellar alignment of cells and matrix deposition. A secondary alignment effect is noted on the PF where a new layer of aligned cells grows over the first layer, but rotated obliquely to the underlying pattern. This layering and rotation of the MSCs resembles the cellular organization observed in native lamellar bone.
Department of Biomedical Engineering, Tufts University, 4 Colby St. Medford, MA 02155, USA.
Lee W
LW
Tien
Eun Seok
ES
Gil
Sang-Hyug
SH
Park
Biman B
BB
Mandal
David L
DL
Kaplan
D000469:Alkaline Phosphatase; D001842:Bone and Bones / Q000166:cytology*; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D010012:Osteogenesis / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D047011:Silk / Q000737:chemistry*; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D047011:Silk; D000469:Alkaline Phosphatase