Distinguishing urothelial carcinoma in the upper urinary tract from benign diseases with hematuria using FISH.
OBJECTIVE
The aims of this study were to evaluate the clinical utility of a fluorescence in situ hybridization (FISH) assay as a non-invasive molecular test to distinguish urothelial carcinoma (UC) in the upper urinary tract (UUT) from benign lesions presenting with hematuria.


METHODS
The chromosomal abnormalities of chromosomes 3, 7, 17 and 9 (p16) in hematuria specimens from 34 patients with UUT-UC and 33 patients with benign disorders were detected using a set of fluorescently labeled DNA probes. The abnormalities of the chromosomes were determined and analyzed between UUT-UC and benign disorders.


RESULTS
Chromosomal abnormalities were detected in 25 of 34 (73.5%) patients with UUT-UC and in 2 of 33 (6.1%) patients with benign disorders (p < 0.001).


CONCLUSIONS
FISH of chromosomes 3, 7, 9 and 17 performed on exfoliated cells from voided urine specimens may serve as a non-invasive tool to distinguish UUT-UC from benign disorders presenting with hematuria.
Department of Pathology, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, PR China.
Jiandong
J
Wang
Jinrong
J
Wu
Libo
L
Peng
Pin
P
Tu
Wanchun
W
Li
Leilei
L
Liu
Wen
W
Cheng
Xuan
X
Wang
Shuigen
S
Zhou
Shanshan
S
Shi
Henghui
H
Ma
Guangming
G
Lu
Xiaojun
X
Zhou
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis / Q000235:genetics*; D002869:Chromosome Aberrations; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D006417:Hematuria / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D019656:Loss of Heterozygosity; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014570:Urologic Diseases / Q000175:diagnosis / Q000235:genetics*; D014571:Urologic Neoplasms / Q000175:diagnosis / Q000235:genetics*; D055815:Young Adult
D019941:Cyclin-Dependent Kinase Inhibitor p16