Centella asiatica protects against UVB-induced HaCaT keratinocyte damage             through microRNA expression changes.
This study aimed to evaluate the protective effects of Centella asiatica (C. asiatica) against ultraviolet B (UVB) damage in human keratinocytes using microRNA (miRNA) expression profiling analysis. Titrated extract of C. asiatica (TECA) demonstrated low cytotoxicity in normal human HaCaT keratinocytes only at low doses (<5 µg/ml). UVB (50 mJ/cm2) irradiation significantly decreased cell viability, and TECA treatment decreased the UVB toxicity. By using miRNA microarrays, we determined that 72 miRNAs had an altered expression following TECA treatment in UVB-irradiated keratinocytes (46 upregulated and 26 downregulated). Using an miRNA target gene prediction tool and Gene Ontology (GO) analysis, we determined that miRNAs with altered expression were functionally related with the inhibition of apoptosis and cell proliferation. Overall, these results provide meaningful information to facilitate the understanding of TECA-mediated UVB protection in human keratinocytes.
Korea Institute for Skin and Clinical Sciences, Konkuk University,                     Seoul 143-701, Republic of Korea.
In-Sook
IS
An
Sungkwan
S
An
Tae-Βoo
TΒ
Choe
Sang-Μo
SΜ
Kang
Jae Ho
JH
Lee
In-Chul
IC
Park
Young-Woo
YW
Jin
Su-Jae
SJ
Lee
Seunghee
S
Bae
D017209:Apoptosis / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D028041:Centella / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D020411:Oligonucleotide Array Sequence Analysis; D010936:Plant Extracts / Q000494:pharmacology*; D011836:Radiation Tolerance / Q000187:drug effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects* / Q000528:radiation effects; D014466:Ultraviolet Rays
C518724:CML-1 plant extract; D035683:MicroRNAs; D010936:Plant Extracts; D011837:Radiation-Protective Agents