Cloning and evolutionary analysis of tobacco MAPK gene family.
The mitogen-activated protein (MAP) kinase cascade is an important signaling module which is involved in biotic and abiotic stress responses as well as plant growth and development. In this study, we identified 17 tobacco MAPKs including 11 novel tobacco MAPK genes that have not been identified before. Comparative analysis with MAPK gene families from other plants, such as Athaliana thaliana, rice and poplar, suggested that tobacco MAPKs (such as NtMPK1, NtMPK3 and NtMPK8) might play similar functions in response to abiotic and biotic stresses. QRT-PCR analysis revealed that a total of 14 NtMPKs were regulated by SA and/or MeJA, suggesting their potential roles involved in plant defense response. In addition, 6 NtMPKs were induced by drought treatment, implying their roles in response to drought stress. Our results indicated that most of tobacco MAPK might be involved in plant defense response, which provides the basis for further analysis on physiological functions of tobacco MAPKs.
The Institute of Agricultural and Life Science, Chongqing University, Chongqing, 400030, China.
Xingtan
X
Zhang
Tingcai
T
Cheng
Genhong
G
Wang
Yafei
Y
Yan
Qingyou
Q
Xia
D000085:Acetates / Q000494:pharmacology; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003517:Cyclopentanes / Q000494:pharmacology; D004790:Enzyme Induction; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics*; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D054883:Oxylipins / Q000494:pharmacology; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D020156:Salicylic Acid / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics*
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C072239:methyl jasmonate; D020928:Mitogen-Activated Protein Kinases; D020156:Salicylic Acid