High resolution copy number analysis of IRF4 translocation-positive diffuse large B-cell and follicular lymphomas.
Translocations affecting chromosome subband 6p25.3 containing the IRF4 gene have been recently described as characteristic alterations in a molecularly distinct subset of germinal center B-cell-derived lymphomas. Secondary changes have yet only been described in few of these lymphomas. Here, we performed array-comparative genomic hybridization and molecular inversion probe microarray analyses on DNA from 12 formalin-fixed paraffin-embedded and two fresh-frozen IRF4 translocation-positive lymphomas, which together with the previously published data on nine cases allowed the extension of copy number analyses to a total of 23 of these lymphomas. All except one case carried chromosomal imbalances, most frequently gains in Xq28, 11q22.3-qter, and 7q32.1-qter and losses in 6q13-16.1, 15q14-22.31, and 17p. No recurrent copy-neutral losses of heterozygosity were observed. TP53 point mutations were detected in three of six cases with loss of 17p. Overall this study unravels a recurrent pattern of secondary genetic alterations in IRF4 translocation-positive lymphomas.
Institute of Human Genetics, University Hospital Schleswig-Holstein Campus Kiel/Christian-Albrechts University, Kiel, Germany.
Itziar
I
Salaverria
Idoia
I
Martin-Guerrero
Birgit
B
Burkhardt
Markus
M
Kreuz
Thorsten
T
Zenz
Ilske
I
Oschlies
Norbert
N
Arnold
Michael
M
Baudis
Susanne
S
Bens
Africa
A
García-Orad
Jasmin
J
Lisfeld
Carsten
C
Schwaenen
Monika
M
Szczepanowski
Swen
S
Wessendorf
Michael
M
Pfreundschuh
Lorenz
L
Trümper
Wolfram
W
Klapper
Reiner
R
Siebert
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D002869:Chromosome Aberrations; D055028:Comparative Genomic Hybridization / Q000379:methods; D056915:DNA Copy Number Variations; D004252:DNA Mutational Analysis; D005260:Female; D018858:Germinal Center / Q000378:metabolism / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D050835:Interferon Regulatory Factors / Q000235:genetics*; D008224:Lymphoma, Follicular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D014178:Translocation, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D055815:Young Adult; D000099218:Interferon Regulatory Factor-4
D050835:Interferon Regulatory Factors; D016159:Tumor Suppressor Protein p53; D000099218:Interferon Regulatory Factor-4