Comparison of immunochromatography, PCR and culture methods for the detection of Campylobacter bacteria.
A total of 463 fecal specimens from human patients were tested for the presence of Camphylobacter by a commercial immunochromatography kit (ImmunoCard STAT! CAMPY), polymerase chain reaction (PCR) and conventional bacteriological (CB) methods. Using culture as the standard of reference, the ImmunoCard STAT! CAMPY assay had a sensitivity of 86% and a specificity of 100%. On the other hand, using PCR as the standard of reference, ImmunoCard STAT! CAMPY assay had a sensitivity of 90.5% and a specificity of 100%.
Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.
Shuvra Kanti
SK
Dey
Shuichi
S
Nishimura
Shoko
S
Okitsu
Satoshi
S
Hayakawa
Masashi
M
Mizuguchi
Hiroshi
H
Ushijima
D002167:Campylobacter / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000175:diagnosis / Q000382:microbiology*; D002846:Chromatography, Affinity / Q000379:methods*; D015169:Colony Count, Microbial / Q000379:methods*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*