Urinary mRNA expression of CCN2/CCN3 as a noninvasive marker for monitoring glomerular structure changes in nondiabetic chronic kidney disease.
BACKGROUND
We investigated whether urinary mRNA of connective tissue growth factor (CCN2) and nephroblastoma overexpressed gene (CCN3) can provide clinical insight into the management of patients with nondiabetic CKD.


METHODS
Urinary mRNA expression of CCN2 and CCN3 were measured by Real-time PCR in 35 CKD patients and 12 controls.


RESULTS
Urinary mRNA of CCN2 and CCN3 were distinctively greater in CKDs than healthy controls. Urinary CCN3/CCN2 mRNA ratio correlated to the degree of glomerular histological changes in those who received renal biopsy.


CONCLUSIONS
Urinary CCN3/CCN2 mRNA ratio may be a useful noninvasive biomarker for evaluating patients with nondiabetic CKD prior to renal biopsy.
Institute of Nephrology, Division of Nephrology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.
Long
L
Chen
Yong-Gui
YG
Wu
Dan
D
Liu
Lin-Li
LL
Lv
Min
M
Zheng
Hai-Feng
HF
Ni
Yu-Han
YH
Cao
Hong
H
Liu
Pei
P
Zhang
Jian-Dong
JD
Zhang
Bi-Cheng
BC
Liu
D000328:Adult; D015415:Biomarkers / Q000652:urine*; D001706:Biopsy; D055513:Connective Tissue Growth Factor / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005919:Glomerular Filtration Rate; D006801:Humans; D007668:Kidney / Q000473:pathology; D007678:Kidney Glomerulus / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D055514:Nephroblastoma Overexpressed Protein / Q000235:genetics*; D011507:Proteinuria / Q000652:urine; D012333:RNA, Messenger / Q000652:urine*; D051436:Renal Insufficiency, Chronic / Q000175:diagnosis / Q000235:genetics / Q000652:urine*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D015415:Biomarkers; C526317:CCN2 protein, human; C526319:CCN3 protein, human; D055514:Nephroblastoma Overexpressed Protein; D012333:RNA, Messenger; D055513:Connective Tissue Growth Factor