1-naphthylhydrazine hydrochloride: a new matrix for the quantification of glucose and homogentisic acid in real samples by MALDI-TOF MS.
BACKGROUND
Due to its strong ultraviolet absorption and low background interference in the low molecular weight region, 1-naphthylhydrazine hydrochloride (NHHC) has been selected as an ideal matrix to detect small molecules by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).


RESULTS
This salt-tolerant matrix could be applied for the high sensitive glucose analysis with an ultra-low limit of detection of 1 amol, and the [glucose+Cl](-) signal can be found even in saturated NaCl solution. With NHHC, glucose in serum and the biomarker homogentisic acid in urine were successfully determined by MALDI-TOF MS in negative ion mode.


CONCLUSIONS
This NHHC-assisted laser desorption/ionization MS method provided a fast and high through-put approach for the small molecule analysis in complex samples, and have a great potential in clinical applications.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Qing
Q
He
Suming
S
Chen
Jianing
J
Wang
Jian
J
Hou
Jiyun
J
Wang
Shaoxiang
S
Xiong
Zongxiu
Z
Nie
D001786:Blood Glucose / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006713:Homogentisic Acid / Q000652:urine*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001786:Blood Glucose; D006834:Hydrazines; D009281:Naphthalenes; C031721:naphthalene; C029424:hydrazine; D006713:Homogentisic Acid