Green chemistry approach for the synthesis and stabilization of biocompatible gold nanoparticles and their potential applications in cancer therapy.
The biological approach to synthesis of AuNPs is eco-friendly and an ideal method to develop environmentally sustainable nanoparticles alternative to existing methods. We have developed a simple, fast, clean, efficient, low-cost and eco-friendly single-step green chemistry approach for the synthesis of biocompatible gold nanoparticles (AuNPs) from chloroauric acid (HAuCl(4)) using a water extract of Eclipta Alba leaves at room temperature. The AuNPs using Eclipta extract have been formed in very short time, even in less than 10 min. The as-synthesized AuNPs were thoroughly characterized by several physico-chemical techniques. The in vitro stability of as-synthesized AuNPs was studied in different buffer solutions. A plausible mechanism for the synthesis of AuNPs by Eclipta extract has been discussed. The biocompatibility of AuNPs was observed by in vitro cell culture assays. Finally, we have designed and developed a AuNPs-based drug delivery system (DDS) (Au-DOX) containing doxorubicin (DOX), a FDA approved anticancer drug. Administration of this DDS to breast cancer cells (MCF-7 and MDA-MB-231) shows significant inhibition of breast cancer cell proliferation compared to pristine doxorubicin. Therefore we strongly believe that the use of Eclipta Alba offers large-scale production of biocompatible AuNPs that can be used as a delivery vehicle for the treatment of cancer diseases.
Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad, AP, India.
Sudip
S
Mukherjee
V
V
Sushma
Sujata
S
Patra
Ayan Kumar
AK
Barui
Manika Pal
MP
Bhadra
Bojja
B
Sreedhar
Chitta Ranjan
CR
Patra
D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002712:Chlorides / Q000737:chemistry; D004317:Doxorubicin / Q000008:administration & dosage / Q000494:pharmacology; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D036601:Eclipta / Q000737:chemistry*; D005260:Female; D006046:Gold / Q000737:chemistry*; D017612:Gold Compounds / Q000737:chemistry; D055772:Green Chemistry Technology / Q000191:economics / Q000379:methods*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D018515:Plant Leaves / Q000737:chemistry
D000903:Antibiotics, Antineoplastic; D002712:Chlorides; D004337:Drug Carriers; D017612:Gold Compounds; D010936:Plant Extracts; D006046:Gold; D004317:Doxorubicin; C024568:gold tetrachloride, acid