Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat.
Fluorescence in situ hybridization (FISH) is a useful tool for physical mapping of chromosomes and studying evolutionary chromosome rearrangements. Here we report a robust method for single-copy gene FISH for wheat. FISH probes were developed from cDNA of cytosolic acetyl-CoA carboxylase (ACCase) gene (Acc-2) and mapped on chromosomes of bread wheat, Triticum aestivum L. (2n = 6x = 42, AABBDD), and related diploid and tetraploid species. Another nine full-length (FL) cDNA FISH probes were mapped and used to identify chromosomes of wheat species. The Acc-2 probe was detected on the long arms of each of the homoeologous group 3 chromosomes (3A, 3B, and 3D), on 5DL and 4AL of bread wheat, and on homoeologous and nonhomoeologous chromosomes of other species. In the species tested, FISH detected more Acc-2 gene or pseudogene sites than previously found by PCR and Southern hybridization analyses and showed presence/absence polymorphism of Acc-2 sequences. FISH with the Acc-2 probe revealed the 4A-5A translocation, shared by several related diploid and polyploid species and inherited from an ancestral A-genome species, and the T. timopheevii-specific 4A(t)-3A(t) translocation.
Wheat Genetic and Genomic Resources Center, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA.
Tatiana V
TV
Danilova
Bernd
B
Friebe
Bikram S
BS
Gill
D000103:Acetyl-CoA Carboxylase / Q000235:genetics*; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D018076:DNA, Complementary; D004171:Diploidy; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D010940:Plant Proteins / Q000235:genetics; D057891:Tetraploidy; D014908:Triticum / Q000235:genetics*
D018076:DNA, Complementary; D010940:Plant Proteins; D000103:Acetyl-CoA Carboxylase