Molecular epidemiology of recent HIV-1 infections in southern Poland.
The genetic diversity of human immunodeficiency virus type 1 (HIV-1) offers an opportunity to track the development of the epidemic across different populations. Viral pol gene fragments from 55 individuals of Polish origin with recent HIV-1 infection identified in 2008-2010 in four Polish cities were analyzed. Viral sequences were compared with sequences from 100 individuals (reference group) infected before 2004. Viral spread among groups with different HIV transmission categories was compared using a phylogenetic approach. The majority of sequences from individuals with recent infection were subtype B (93%) within which four transmission clusters (18% of samples) were detected. Samples from men infected through sex between men and from persons infected through injecting drugs were broadly separated (P < 0.0001), while samples from individuals infected by heterosexual contacts were dispersed uniformly within phylogenetic tree (P = 0.244) inferred from viral sequences derived from individuals infected recently and the reference group. The percentage of samples from persons infected by heterosexual contacts which clustered with samples from men infected through sex between men was not significantly higher for those with recent infection (47%), compared to the reference group (36%). In conclusion, men infected by sex between men and individuals infected through injecting drugs appear to form separate HIV transmission networks in Poland. The recent spread of HIV-1 among persons infected with subtype B by heterosexual contacts appears to be linked to both these groups.
Department and Institute of Microbiology and Virology, The School of Pharmacy and Division of Laboratory Medicine, Medical University of Silesia, Katowice, Poland.
Joanna
J
Smoleń-Dzirba
Magdalena
M
Rosińska
Piotr
P
Kruszyński
Jolanta
J
Bratosiewicz-Wąsik
Janusz
J
Janiec
Marek
M
Beniowski
Monika
M
Bociąga-Jasik
Elżbieta
E
Jabłonowska
Bartosz
B
Szetela
Kholoud
K
Porter
Tomasz J
TJ
Wąsik
D000328:Adult; D001483:Base Sequence; D005260:Female; D005814:Genes, Viral; D014644:Genetic Variation; D015658:HIV Infections / Q000175:diagnosis / Q000453:epidemiology* / Q000635:transmission / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D020010:Heterosexuality; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D010802:Phylogeny; D011044:Poland / Q000453:epidemiology; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics; D018709:Statistics, Nonparametric; D055815:Young Adult; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics
D012367:RNA, Viral; D054302:pol Gene Products, Human Immunodeficiency Virus