A comparative proteomic analysis provides insights into pigment biosynthesis in brown color fiber.
A comparative proteomic analysis was performed to identify the differences between brown cotton fiber and a white near-isogenic line, and 78 differential spots were identified at three time points (12-, 18-, and 24-day post-anthesis [DPA]) using MALDI-TOF/TOF. Our data illustrate several aspects of pigment synthesis and fiber development in brown color fiber (BCF). First, 21 spots were associated with secondary metabolism; 15 of these with high abundance in BCF were involved in flavonoid biosynthesis. Second, several spots with lower abundance in BCF were found. Thirteen spots were related to energy/carbohydrate metabolism; in particular, spots related to the glycolytic pathway exhibited lower abundance in 12 DPA BCF. Several spots related to redox homeostasis, cytoskeleton, and protein metabolism also showed lower abundance in BCF, including proteins that are critical for fiber development, such as ascorbate peroxidase, superoxide dismutase, actin, annexin and heat shock protein. Third, several proteins such as leucine aminopeptidase preprotein and progesterone-5-beta-reductase were newly identified proteins in cotton fibers. These findings demonstrated the presence of a complicated metabolic network in BCF and advanced our understanding of the molecular mechanisms of pigment biosynthesis in colored cotton, which will provide new insight for the development of new color types by genetic manipulation.
The Key Laboratory of Oasis Eco-agriculture, Agriculture College of Shihezi University, Shihezi, Xinjiang Province, 832003, PR China.
Yan-Jun
YJ
Li
Xin-Yu
XY
Zhang
Fu-Xin
FX
Wang
Chun-Lin
CL
Yang
Feng
F
Liu
Gui-Xian
GX
Xia
Jie
J
Sun
D050260:Carbohydrate Metabolism / Q000502:physiology; D032561:Cotton Fiber; D004734:Energy Metabolism / Q000502:physiology; D005419:Flavonoids / Q000096:biosynthesis*; D003368:Gossypium / Q000378:metabolism*; D010860:Pigments, Biological / Q000096:biosynthesis*; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D005419:Flavonoids; D010860:Pigments, Biological; D010940:Plant Proteins