Se-derivatized RNAs for X-ray crystallography.
Selenium derivatization of RNA is an important strategy for crystal structure determination and functional studies of noncoding RNAs and protein-RNA interactions. We describe here the synthesis of nucleoside 5'-(α-P-seleno)-triphosphate analogs (Se-NTPs) and their use in vitro transcription and purification of Se-derivatized RNA samples (phosphoroselenoate RNA, PSe-RNA).
Department of Chemistry, Georgia State University, Atlanta, GA, USA.
Lina
L
Lin
Zhen
Z
Huang
D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel; D012313:RNA / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D012265:Ribonucleotides / Q000737:chemistry / Q000302:isolation & purification; D012643:Selenium / Q000737:chemistry*; D014158:Transcription, Genetic
D012265:Ribonucleotides; D012313:RNA; D012643:Selenium