Antibacterial mechanism of plasma sprayed Ca2ZnSi2O7 coating against Escherichia coli.
In our previous work, antibacterial activity of plasma sprayed Ca(2)ZnSi(2)O(7) coating has been demonstrated. However, antibacterial mechanism of Ca(2)ZnSi(2)O(7) coating still remains undefined. In this study, Escherichia coli (E. coli), a kind of Gram-negative bacteria, was chosen to investigate the interactions between the bacterium and Ca(2)ZnSi(2)O(7) coating. Scanning electron microscopy and transmission electron microscopy micrographs exhibited that the morphologies of E. coli on the coating changed with treatment time, from initial slight disturbance to the disruption of cell wall and drastic distortion of bacterial interior where a remarkable material-light region was formed in the center and condensed deoxyribonucleic acid (DNA) molecules were found. Disturbances of cytoplasmic membrane were observed by two-photon confocal microscopy and confirmed by leakage of intracellular potassium ion (K(+)). Results suggest that the destruction of cell wall and the loss of replication ability of DNA molecules are two major reasons causing death of E. coli.
Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, People's Republic of China.
Kai
K
Li
Youtao
Y
Xie
Liping
L
Huang
Heng
H
Ji
Xuebin
X
Zheng
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002118:Calcium / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D058626:Plasma Gases; D011188:Potassium / Q000737:chemistry; D012825:Silicon / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry
D000900:Anti-Bacterial Agents; D058626:Plasma Gases; D015032:Zinc; D011188:Potassium; D002118:Calcium; D012825:Silicon