Expression profiles of Th17 pathway related genes in human systemic lupus erythematosus.
Recently, evidence is emerging that inappropriate regulation of type 17 T helper cells (Th17) plays a fundamental role in the development of many autoimmune diseases including systemic lupus erythematosus (SLE). However, the role of Th17-related cytokines in SLE remains elusive. To further investigate the role and imbalance of Th17-related cytokines in the pathogenesis of SLE. A Quantitative RT-PCR Array (Human Th17 for Autoimmunity & Inflammation PCR Array) analyses were performed to study Th17-related genes expression in peripheral white blood cells of 25 new-onset patients with SLE and 15 healthy subjects. When gene expression for SLE patients was compared to the mean of normal controls, among the 84 target genes related to Th17 pathway, 7 (CXCL1, ICAM1, IL10, IL5, IL8, ISG20, JAK2,) were upregulated and 6 (CD28, CD40LG, S1PR1, IL17RE, IL23R, RORC) downregulated. However, comparisons of mRNA expression of Th17 related cytokines between lupus nephritis (LN) patients and SLE patients without nephritis (SLE non LN) showed no significant difference. In conclusion, SLE patients and normal controls showed different expression of a few genes in Th17 pathway, indicating that the pathway may be involved in the pathogenesis of SLE.
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei 230032, Anhui, People's Republic of China. panhaifeng1982@gmail.com
Hai-Feng
HF
Pan
Rui-Xue
RX
Leng
Chen-Chen
CC
Feng
Xiang-Pei
XP
Li
Gui-Mei
GM
Chen
Bao-Zhu
BZ
Li
Wang-Dong
WD
Xu
Song Guo
SG
Zheng
Dong-Qing
DQ
Ye
D000328:Adult; D015551:Autoimmunity / Q000235:genetics / Q000276:immunology; D016022:Case-Control Studies; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D015398:Signal Transduction; D058504:Th17 Cells / Q000276:immunology* / Q000378:metabolism*; D055815:Young Adult
D016207:Cytokines