Antimicrobial efficacy of a human β-defensin-3 peptide using an Enterococcus faecalis dentine infection model.
OBJECTIVE
To assess the antibacterial efficacy of a human β-defensin-3 (HBD3) peptide against Enterococcus faecalis biofilms.


METHODS
Standardized human dentine blocks were infected with E. faecalis ATCC 29212 for 3 weeks. Aqueous calcium hydroxide paste (n = 12, CH), a 2% chlorhexidine gel (n = 12, CHX), an HBD3 peptide gel (n = 12) and saline (n = 12) were tested as experimental groups. A mismatched peptide gel group (n = 12, MP) and sterilized but noninoculated block group (n = 12) were included as controls. After 1 week of medication, the dentinal samples at the depth of 200 and 400 μm were collected from medicated canal lumens. Bacterial growth was assessed by spectrophotometric analysis of optical density (OD) after 72 h of incubation. Statistical analysis was performed with repeated-measures anova and Tukey's post hoc test.


RESULTS
The HBD3 group was associated with significantly lower OD values (P < 0.05) than the CH or CHX groups at both depths. The CH group did not differ significantly from MP or Saline group at either depth (P > 0.05). There was no significant difference (P > 0.05) in the OD values of the inner (200 μm) and outer (400 μm) dentinal samples for any group.


CONCLUSIONS
The HBD3 peptide inhibited the growth of E. faecalis biofilms in infected dentine blocks.
Department of Conservative Dentistry, Dental Research Institute and BK21 program, Seoul National University Dental Hospital, Seoul National University School of Dentistry, Seoul, Korea.
J-K
JK
Lee
Y-J
YJ
Park
K-Y
KY
Kum
S H
SH
Han
S-W
SW
Chang
B
B
Kaufman
J
J
Jiang
Q
Q
Zhu
K
K
Safavi
L
L
Spångberg
D000890:Anti-Infective Agents / Q000627:therapeutic use*; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use; D018441:Biofilms / Q000187:drug effects*; D002126:Calcium Hydroxide / Q000627:therapeutic use; D002710:Chlorhexidine / Q000627:therapeutic use; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000382:microbiology; D003804:Dentin / Q000187:drug effects / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000187:drug effects*; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy*; D006801:Humans; D006813:Humidity; D008422:Materials Testing; D009391:Nephelometry and Turbidimetry; D012388:Root Canal Irrigants / Q000627:therapeutic use; D012965:Sodium Chloride; D013053:Spectrophotometry; D013696:Temperature; D013997:Time Factors; D023083:beta-Defensins / Q000627:therapeutic use*
D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local; C422950:DEFB103A protein, human; D012388:Root Canal Irrigants; D023083:beta-Defensins; D012965:Sodium Chloride; D002126:Calcium Hydroxide; D002710:Chlorhexidine