Modulation of the immuno-coagulative response in a pneumococcal infection in malnourished mice nasally treated with Lactobacillus casei.
We studied the systemic effects of the intranasal administration of Lactobacillus casei on the immuno-coagulative response in pneumoccocal infection in immunocompromised mice. Weaned mice consumed a protein-free diet (PFD) for 21 days and were therefore malnourished. Malnourished mice were fed a balanced conventional diet (BCD) for 7 days (BCD group) or a BCD for 7 days with nasal administration of viable L. casei on days 6 and 7 (BCD+LcN group). The malnourished control mice (MNC) received a PFD, whereas the well-nourished control mice (WNC) continually consumed a BCD. At the end of the treatment period, the mice were infected with Streptococcus pneumoniae. At different times after infection, we analysed the following parameters: global coagulation system, activation of coagulation, coagulation inhibitors, platelet count, leukocyte count and myeloperoxidase (MPO) activity, total proteins, albumin and acute phase proteins (APPs). The MNC group showed greater impairment in the coagulation tests and an increase in the positive APPs. These parameters were normalized by the L. casei treatment. However, the number of leukocytes, decreased by malnutrition, was improved only by the administration of L. casei. After infection, the BCD+LcN group showed similar results to those of the WNC group for most of the haemostatic parameters. The BCD+LcN group did not show significant variations in the prothrombin time or in the level of anticoagulant protein C, but showed higher levels of fibrinogen, platelets, albumin, leukocytes and MPO activity compared with the different experimental groups. The intranasal administration of L. casei was effective in modulating the pro-inflammatory aspects of coagulation without affecting coagulation itself.
Bioquímica Clínica I, Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Balcarce 747, CP 4000, San Miguel de Tucumán, Tucumán, Argentina.
Hortensia
H
Zelaya
Bioquímica Clínica I, Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Balcarce 747, CP 4000, San Miguel de Tucumán, Tucumán, Argentina.
Jonathan
J
Laiño
Bioquímica Clínica I, Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Balcarce 747, CP 4000, San Miguel de Tucumán, Tucumán, Argentina.
Cecilia
C
Haro
Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, CP 4000, San Miguel de Tucumán, Tucumán, Argentina.|Bioquímica Clínica I, Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Balcarce 747, CP 4000, San Miguel de Tucumán, Tucumán, Argentina.
Susana
S
Alvarez
Bioquímica Clínica I, Instituto de Bioquímica Aplicada, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Balcarce 747, CP 4000, San Miguel de Tucumán, Tucumán, Argentina.
Graciela
G
Agüero
D000209:Acute-Phase Proteins / Q000378:metabolism; D000818:Animals; D001777:Blood Coagulation; D001835:Body Weight; D016867:Immunocompromised Host; D007780:Lacticaseibacillus casei; D008168:Lung / Q000382:microbiology / Q000473:pathology; D008297:Male; D044342:Malnutrition / Q000276:immunology* / Q000628:therapy; D051379:Mice; D010976:Platelet Count; D011008:Pneumococcal Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D019936:Probiotics / Q000627:therapeutic use*; D013296:Streptococcus pneumoniae
D000209:Acute-Phase Proteins