In situ AFM analysis investigating disassembly of DNA nanoparticles and nano-films.
Synthetic vector-based gene delivery systems continue to gain strength as viable alternatives to viral vectors due to safety and other concerns. DNA release dynamics is key to the understanding and control of gene delivery from nano-systems. Here we describe atomic force microscope application to the understanding of DNA release dynamics from bioreducible polycation-based nano-systems. The two nano-systems are polyplex nanoparticles and layer-by-layer films.
Department of Chemical Engineering and Material Sciences, Wayne State University, Detroit, MI, USA.
Yi
Y
Zou
Lei
L
Wan
Jenifer
J
Blacklock
David
D
Oupicky
Guangzhao
G
Mao
D000595:Amino Acid Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D018014:Gene Transfer Techniques; D018625:Microscopy, Atomic Force / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes
D004247:DNA; D004337:Drug Carriers; D010455:Peptides; D011073:Polyamines; D000071228:Polyelectrolytes; C531249:Poly(amidoamine)