Effect of adenosine modified with a boron cluster pharmacophore on reactive oxygen species production by human neutrophils.
Methods for the synthesis of adenosine/boron cluster conjugates are proposed and the potential of the obtained derivatives to modulate neutrophil activity, especially reactive oxygen species (ROS) production in vitro, is described. An efficient inhibition of ROS production in activated neutrophils by adenosine modified at the 2'-C and 6-N positions with a para-carborane cluster (C(2)B(10)H(11)) was discovered. The high affinity of the selected compounds for adenosine receptor A(2A) was established. These results are in agreement with the possible involvement of receptor A(2A) in the biological activities of adenosine/boron cluster conjugates. This study extends the range of innovative molecules available for testing as agents affecting inflammatory processes.
Laboratory of Experimental Immunology, Institute of Medical Biology PAS, 106 Lodowa St., Lodz, Poland.
Katarzyna
K
Bednarska
Agnieszka B
AB
Olejniczak
Agnieszka
A
Piskala
Magdalena
M
Klink
Zofia
Z
Sulowska
Zbigniew J
ZJ
Lesnikowski
D000241:Adenosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D001895:Boron / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D043705:Receptor, Adenosine A2A / Q000737:chemistry / Q000378:metabolism
D017382:Reactive Oxygen Species; D043705:Receptor, Adenosine A2A; D000241:Adenosine; D001895:Boron