Flow cytometry-based cell type-specific assessment of target regulation by pulmonary siRNA delivery.
Pulmonary siRNA delivery has attracted strong interest and has been reported to successfully mediate target gene knockdown in a number of disease models. However, the nature of the epithelial cells that eventually take up siRNA and the question if other lung cell types may also be transfected have so far been neglected. Therefore, we describe here a flow cytometry-based method using transgenic enhanced green fluorescence protein-expressing mice (EGFP mice) for the differentiation of transfected lung cell populations based on their antigen expression.
Department of Pharmaceutical Sciences, Wayne State University, Detroit, MI, USA. Olivia.Merkel@staff.uni-marburg.de
Olivia M
OM
Merkel
Leigh M
LM
Marsh
Holger
H
Garn
Thomas
T
Kissel
D000818:Animals; D001483:Base Sequence; D018893:Bronchoalveolar Lavage; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D055785:Gene Knockdown Techniques; D049452:Green Fluorescent Proteins / Q000172:deficiency / Q000235:genetics; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000378:metabolism*; D008297:Male; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D014162:Transfection / Q000379:methods*
D034741:RNA, Small Interfering; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins