A label-free electrical detection of exosomal microRNAs using microelectrode array.
We report a method for detecting microRNAs encapsulated in exosomes using a microelectrode array in semiconductor-based potentiometry after RT-PCR. The inherent miniaturization of the electrical biosensor meets requirements for massively parallel analysis of circulating microRNA as a noninvasive biomarker.
Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Tatsuro
T
Goda
Kozue
K
Masuno
Junko
J
Nishida
Nobuyoshi
N
Kosaka
Takahiro
T
Ochiya
Akira
A
Matsumoto
Yuji
Y
Miyahara
D015374:Biosensing Techniques; D055354:Exosomes / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D008839:Microelectrodes; D011199:Potentiometry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012666:Semiconductors
D035683:MicroRNAs