Gut microbial metabolism of polyphenols from black tea and red wine/grape juice is source-specific and colon-region dependent.
The colonic microbial degradation of a polyphenol-rich black tea extract (BTE) and red wine/grape juice extract (RWGE) was compared in a five-stage in vitro gastrointestinal model (TWINSHIME). Microbial metabolism of BTE and RWGE polyphenols in the TWINSHIME was studied subsequently in single- and continuous-dose experiments. A combination of liquid or gas chromatography with mass spectrometry (LC-MS or GC-MS) and NMR-based metabolic profiling was used to measure selected parent polyphenols, their microbial degradation into phenolic acids, and the production of short-chain fatty acids (SCFAs) in different colon compartments. Acetate production was increased by continuous feeding of BTE but not RWGE. During RWGE feeding, gallic acid and 4-hydroxyphenylpropionic acid remained elevated throughout the colon, while during BTE feeding, they were consumed in the distal colon, while 3-phenylpropionic acid was strongly produced. Gut microbial production of phenolics and SCFAs is dependent on colon location and polyphenol source, which may influence potential health benefits.
Unilever R&D Vlaardingen, Post Office Box 114, 3130 AC Vlaardingen, The Netherlands. ferdivan.dorsten@Unilever.com
F A
FA
van Dorsten
S
S
Peters
G
G
Gross
V
V
Gomez-Roldan
M
M
Klinkenberg
R C
RC
de Vos
E E
EE
Vaughan
J P
JP
van Duynhoven
S
S
Possemiers
T
T
van de Wiele
D M
DM
Jacobs
D001419:Bacteria / Q000378:metabolism*; D028241:Camellia sinensis / Q000737:chemistry / Q000378:metabolism*; D003106:Colon / Q000378:metabolism* / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000378:metabolism* / Q000382:microbiology*; D006801:Humans; D054892:Metagenome; D008954:Models, Biological; D010936:Plant Extracts / Q000032:analysis / Q000378:metabolism*; D059808:Polyphenols / Q000378:metabolism*; D013045:Species Specificity; D013662:Tea / Q000378:metabolism; D027843:Vitis / Q000737:chemistry / Q000378:metabolism*; D014920:Wine / Q000032:analysis*
D010936:Plant Extracts; D059808:Polyphenols; D013662:Tea