Tenogenic differentiation of equine mesenchymal progenitor cells under indirect co-culture.
OBJECTIVE
Adult bone marrow mesenchymal stem cells (BM-MSCs) are a potential cell source for tendon repair in direct cell therapy and tissue engineering investigations. The purpose of this study was to evaluate the tenogenic induction of undifferentiated BM-MSCs under indirect co-culture technique with trimmed native tendon tissue. Since the horse represents a preferred species to study tendon regenerative strategies, this work was conducted on equine BM-MSCs.


METHODS
Equine BM-MSCs were co-cultured in a transwell system with tendon tissue fragments. The BM-MSC tenogenic differentiation was evaluated by cytochemical staining and real time PCR for gene expression. Cell viability in tendon fragments and cultured cells was analyzed.


RESULTS
Our results indicate that under indirect co-culture with native and healthy tendon tissue the BM-MSCs expressed tendon-specific markers such as decorin, tenomodulin, tenascin-C, and collagen type I. They also retained a tenocyte-like phenotype during monolayer culture.


CONCLUSIONS
Data are very encouraging for future in vitro investigations into committing cells to the tenogenic lineage without adding growth factors or serum to the culture medium for both cell therapy and tissue engineering.
Reproduction Unit, Department of Veterinary Clinical Science, University of Milan, Lodi, Italy. arianna.lovati@grupposandonato.it
Arianna B
AB
Lovati
Bruna
B
Corradetti
Fausto
F
Cremonesi
Davide
D
Bizzaro
Anna Lange
AL
Consiglio
D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D018920:Coculture Techniques; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D058575:Decorin / Q000235:genetics / Q000378:metabolism; D006736:Horses; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D012333:RNA, Messenger / Q000378:metabolism; D019063:Tenascin / Q000235:genetics / Q000378:metabolism; D013710:Tendons / Q000166:cytology*; D023822:Tissue Engineering
D024042:Collagen Type I; D058575:Decorin; D012333:RNA, Messenger; D019063:Tenascin