[Cloning and expression of triosephosphate isomerase gene of Boophilus microplus].
Total RNA was extracted from adult Boophilus microplus. RT-PCR was used to amplify the gene and the fragment was subcloned into the expression vector pET-28a. The cloned gene was expressed in E. coli Rosseta (DE3), induced by IPTG, and identified by SDS-PAGE. The results showed that the triosephosphate isomerase (tim) gene of B. microplus has 750bp and encodes 249 amino acids (GenBank No. JX112888). The cloned tim gene shares 99% homology with that in tick embryos. The relative molecular weight (M(r)) of the expressed recombinant protein is about 27 000.
School of Medicine, Shenzhen University, Shenzhen 518060, China.
Yi-Lin
YL
Liang
Pan
P
Gao
Ze-Kai
ZK
Ke
Xin-Ping
XP
Luo
Zhi-Gang
ZG
Liu
D000818:Animals; D003001:Cloning, Molecular; D015870:Gene Expression; D048494:Rhipicephalus / Q000201:enzymology / Q000235:genetics*; D014305:Triose-Phosphate Isomerase / Q000235:genetics*
D014305:Triose-Phosphate Isomerase