[Preliminary study on the difference in immune protection between Echinococcus granulosus recombinant ferritin and recombinant mMDH].
OBJECTIVE
To evaluate the immunoprotective activity of the Egrecombinant ferritin and Egrecombinant mMDH proteins in mice.


METHODS
Thirty ICR mice were divided into 3 groups and immunized by injection of adjuvant-emulsified rEgferritin, rEgmMDH and PBS, respectively, in multiple spots at back, for 3 times with an interval of 2 wk. Two weeks after the last immunization, the mice of the 3 groups were infected intraperitoneally with 0.1 ml suspension containing about 1 500 Echinococcus granulosus (Eg) protoscoleces. The mice were sacrificed 22 wk after infection and the Eg cysts were collected and measured. Spleens were taken for detecting CD4+ T cells and CD8+ T cells and ratio calculated.


RESULTS
Eg cysts were found in 30% (3/10) of the mice in the rEgferritin group with 5 cysts altogether; cysts were received in all the mice in the rEgmMDH group with 118 cysts totally; and cysts were found in 7 of 9 mice in the PBS control with 35 cysts totally. The mice in the rEgferritin group showed an 84.7% protection but revealed no protection in the rEgmMDH group. The CD4+ T cells were significantly higher in the rEgferritin group than the control, but no statistical difference was found in CD8+ T cells and CD4+/CD8+ ratio between the 2 groups. There was no considerable change in the T cells and ratio in the rEgmMDH group compared to the control.


CONCLUSIONS
The Egrecombinant ferritin can inhibit the growth of Eg while the Egrecombinant mMDH seems promoting its growth in mice.
Medical Scientific Center, Ningxia Medical University, Yinchuan 750004, China.
Li-Hua
LH
Liu
Jia-Qing
JQ
Zhao
Bo
B
Jiang
Chang-Yi
CY
Chen
Yuan
Y
Wang
Ya-Na
YN
Wang
Jie
J
Wang
Shu-Jing
SJ
Wang
Wei
W
Zhao
D000818:Animals; D048209:Echinococcus granulosus / Q000276:immunology*; D005260:Female; D005293:Ferritins / Q000276:immunology*; D008291:Malate Dehydrogenase / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D024101:Mitochondrial Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*
D024101:Mitochondrial Proteins; D011994:Recombinant Proteins; D005293:Ferritins; D008291:Malate Dehydrogenase