Metabolite profiling of Curcuma species grown in different regions using 1H NMR spectroscopy and multivariate analysis.
Curcuma is used to treat skin diseases and colic inflammatory disorders, and in insect repellants and antimicrobial and antidiabetic medications. Two Curcuma species (C. aromatica and C. longa) grown in Jeju-do and Jin-do were used in this study. Methanolic extracts were analyzed by (1)H NMR spectroscopy, and metabolite profiling coupled with multivariate analysis was applied to characterize the differences between species or origin. PCA analysis showed significantly greater differences between species than origins, and the metabolites responsible for the differences were identified. The concentrations of sugars (glucose, fructose, and sucrose) and essential oils (eucalyptol, curdione, and germacrone) were significantly different between the two species. However, the samples from Jeju-do and Jin-do were different mainly in their concentrations of organic acids (fumarate, succinate, acetate, and formate) and sugars. This study demonstrates that NMR-based metabolomics is an efficient method for fingerprinting and determining differences between Curcuma species or those grown in different regions.
Integrated Metabolomics Research Group, Seoul Center, Korea Basic Science Institute, Seoul 136-713, Republic of Korea.
Youngae
Y
Jung
Jueun
J
Lee
Ho Kyoung
HK
Kim
Byeong Cheol
BC
Moon
Yunui
Y
Ji
Do Hyun
DH
Ryu
Geum-Sook
GS
Hwang
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D030024:Curcuma / Q000737:chemistry* / Q000254:growth & development; D055442:Metabolome; D019906:Nuclear Magnetic Resonance, Biomolecular; D009822:Oils, Volatile / Q000032:analysis; D025341:Principal Component Analysis; D056910:Republic of Korea
D000596:Amino Acids; D002241:Carbohydrates; D009822:Oils, Volatile