In vitro cytotoxicity and in vivo osseointergration properties of compression-molded HDPE-HA-Al2O3 hybrid biocomposites.
The aim of this study was to investigate the in vivo biocompatibility in terms of healing of long segmental bone defect in rabbit model as well as in vitro cytotoxicity of eluates of compression-molded High density polyethylene (HDPE)-hydroxyapatite (HA)-aluminum oxide (Al2O3) composite-based implant material. Based on the physical property in terms of modulus and strength properties, as reported in our recent publication, HDPE-40 wt % HA and HDPE-20 wt % HA-20 wt % Al2O3 hybrid composites were used for biocompatibility assessment. Osteoblasts cells were cultured in conditioned media, which contains varying amount of composite eluate (0.01, 0.1, and 1.0 wt %). In vitro, the eluates did not exhibit any significant negative impact on proliferation, mineralization or on morphology of human osteoblast cells. In vivo, the histological assessment revealed neobone formation at the bone/implant interface, characterized by the presence of osteoid and osteoblasts. The observation of osteoclastic activity indicates the process of bone remodeling. No inflammation to any noticeable extent was observed at the implantation site. Overall, the combination of in vitro and in vivo results are suggestive of potential biomedical application of compression-molded HDPE- 20 wt % HA- 20 wt % Al2O3 composites to heal long segmental bone defects without causing any toxicity of bone cells.
Department of Materials Science and Engineering, Laboratory for Biomaterials, Indian Institute of Technology, Kanpur 208016, Uttra Pradesh, India.
Garima
G
Tripathi
Julie E
JE
Gough
Amit
A
Dinda
Bikramjit
B
Basu
D000537:Aluminum Oxide / Q000494:pharmacology*; D000818:Animals; D000880:Anthraquinones / Q000378:metabolism; D001672:Biocompatible Materials / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects / Q000648:ultrastructure; D002113:Calcification, Physiologic / Q000187:drug effects; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019245:Compressive Strength; D017077:Culture Media, Conditioned / Q000494:pharmacology; D017886:Durapatite / Q000494:pharmacology*; D006801:Humans; D020007:Implants, Experimental; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration / Q000187:drug effects*; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010078:Oxazines / Q000378:metabolism; D020959:Polyethylene / Q000494:pharmacology*; D011817:Rabbits; D014961:X-Ray Diffraction; D014966:Xanthenes / Q000378:metabolism
D000880:Anthraquinones; D001672:Biocompatible Materials; D017077:Culture Media, Conditioned; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin; C010078:alizarin; D020959:Polyethylene; D017886:Durapatite; D000537:Aluminum Oxide