Shear wave elastography quantification of blood elasticity during clotting.
Deep venous thrombosis (DVT) affects millions of people worldwide. A fatal complication occurs when the thrombi detach and create a pulmonary embolism. The diagnosis and treatment of DVT depends on clot's age. The elasticity of thrombi is closely related to its age. Blood was collected from pigs and anticoagulated using ethylenediaminetetraacetic acid (EDTA). Coagulation was initiated using calcium ions. Supersonic shear wave imaging was used to generate shear waves using 100 μs tone bursts of 8 MHz. Tracking of the shear waves was done by ultrafast imaging. Postprocessing of the data was done using Matlab(®). Two-dimensional (2-D) maps of elasticity were obtained by calculating the speed of shear wave propagation. Elasticity varied with time from around 50 Pa at coagulation to 1600 Pa at 120 min after which the elasticity showed a natural decreased (17%) because of thrombolytic action of plasmin. Ejection of the serum from the clot showed a significant decrease in the elasticity of the clot next to the liquid pool (65% decrease), corresponding to the detachment of the clot from the beaker wall. The use of a thrombolytic agent (Urokinase) on the coagulated blood decreased the shear elasticity close to the point of injection, which varied with time and distance. Supersonic imaging proved to be useful mapping the 2-D clot's elasticity. It allowed the visualization of the heterogeneity of mechanical properties of thrombi and has potential use in predicting thrombi breakage as well as in monitoring thrombolytic therapy.
Institut Langevin, Ondes et Images, ESPCI ParisTech, CNRS UMR7587, INSERM U979, Paris, France. bmiguelitoo@gmail.com
Miguel
M
Bernal
Jean-Luc
JL
Gennisson
Patrice
P
Flaud
Mickael
M
Tanter
D000818:Animals; D001777:Blood Coagulation / Q000502:physiology; D004548:Elasticity; D054459:Elasticity Imaging Techniques / Q000379:methods; D066298:In Vitro Techniques; D015203:Reproducibility of Results; D034421:Sus scrofa