BioFET-SIM web interface: implementation and two applications.
We present a web interface which allows us to conveniently set up calculations based on the BioFET-SIM model. With the interface, the signal of a BioFET sensor can be calculated depending on its parameters, as well as the signal dependence on pH. As an illustration, two case studies are presented. In the first case, a generic peptide with opposite charges on both ends is inverted in orientation on a semiconducting nanowire surface leading to a corresponding change in sign of the computed sensitivity of the device. In the second case, the binding of an antibody/antigen complex on the nanowire surface is studied in terms of orientation and analyte/nanowire surface distance. We demonstrate how the BioFET-SIM web interface can aid in the understanding of experimental data and postulate alternative ways of antibody/antigen orientation on the nanowire surface.
Department of Chemistry, University of Copenhagen, Copenhagen, Denmark. martin@chem.ku.dk
Martin R
MR
Hediger
Jan H
JH
Jensen
Luca
L
De Vico
D000465:Algorithms; D000906:Antibodies / Q000737:chemistry / Q000378:metabolism; D000936:Antigen-Antibody Complex / Q000737:chemistry / Q000378:metabolism; D000941:Antigens / Q000737:chemistry / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods / Q000592:standards*; D020407:Internet; D008958:Models, Molecular; D053770:Nanowires / Q000592:standards*; D011485:Protein Binding; D011487:Protein Conformation; D015203:Reproducibility of Results; D055672:Static Electricity; D013499:Surface Properties; D014584:User-Computer Interface
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens