Differential response of macrophages to core-shell Fe3O4@Au nanoparticles and nanostars.
Murine RAW 264.7 cells were exposed to spheroidal core-shell Fe(3)O(4)@Au nanoparticles (SCS-NPs, ca. 34 nm) or nanostars (NSTs, ca. 100 nm) in the presence of bovine serum albumin, with variable effects observed after macrophagocytosis. Uptake of SCS-NPs caused macrophages to adopt a rounded, amoeboid form, accompanied by an increase in surface detachment. In contrast, the uptake of multibranched NSTs did not induce gross changes in macrophage shape or adhesion, but correlated instead with cell enlargement and signatures of macrophage activation such as TNF-α and ROS. MTT assays indicate a low cytotoxic response to either SCS-NPs or NSTs despite differences in macrophage behavior. These observations show that differences in NP size and shape are sufficient to produce diverse responses in macrophages following uptake.
Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907-2084, USA.
Wei
W
Xia
Hyon-Min
HM
Song
Qingshan
Q
Wei
Alexander
A
Wei
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D010316:Particle Size; D010587:Phagocytosis / Q000187:drug effects; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism
D012709:Serum Albumin; D006046:Gold; D052203:Ferrosoferric Oxide