Neurophysiologic responses of peripheral nerve to repeated episodes of anoxia.
OBJECTIVE
Determine the effects of serial episodes of anoxia in an in vitro peripheral nerve preparation.


METHODS
The nerve action potential (NAP) from rat sciatic nerve was recorded during 5 cycles of anoxia and reperfusion. Multiple NAP parameters were analyzed as well as stimulus response curves.


RESULTS
The amplitude of the NAP declined to half baseline in 865 s on the first cycle of anoxia and recovered to half baseline during recovery in 470 s. These times increased with successive cycles of anoxia. The current required to produce a half maximal NAP showed a variable initial decrease before increasing with anoxia. The paired-pulse response showed a decline at 2-3 ms interstimulus interval during anoxia but was less dependent of interstimulus interval during recovery. NAP amplitude and velocity decrease over successive cycles of anoxia at a rate greater than in the absence of anoxia.


CONCLUSIONS
The NAP declines slowly when peripheral nerve is exposed to anoxia but returns at least twice as quickly when re-exposed to oxygen. Short periods of anoxia produce long lasting changes in the nerve suggesting greater resistance to anoxia. With serial episodes of anoxia there is gradual NAP amplitude reduction and increase in duration and latency.


CONCLUSIONS
Anoxic-preconditioning appears in isolated peripheral nerve.
Winthrop University Hospital, Mineola, NY 11530, USA. mmstecker@gmail.com
Mark
M
Stecker
Jacob
J
Wolfe
Matthew
M
Stevenson
D000200:Action Potentials / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D004558:Electric Stimulation; D000860:Hypoxia / Q000503:physiopathology*; D010100:Oxygen / Q000494:pharmacology; D010525:Peripheral Nerves / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion; D012584:Sciatic Nerve / Q000502:physiology; D013696:Temperature
D010100:Oxygen