In vitro biological study of gelatin/PLG nanocomposite using MCF-7 breast cancer cells.
This work aimed to evaluate new materials to be carried out as scaffolds using breast cancer cells MCF-7. These new nanocomposites were prepared through blending of gelatin with poly (DL-lactide-co-glycolide) (PLG) in presence of titanium nanowires (TiO2 ) and cartilage powder (CP). The prepared nanomaterials were characterized using Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscope, and transmitting electron microscope. Moreover, the MCF-7 cells were in vitro tested with apoptosis/necrosis assay, micronucleus test, and DNA fragmentation and MMT assay. TiO2 nanowires and CP particles have diameters around 28-128 and 17-20 nm, respectively. These were coated with gelatin matrix. Seeding of MCF-7 cells with the prepared nanomaterials revealed high cell attachment to their surfaces and they were viable after 72 h. It has been shown that the prepared nanocomposites did not induce necrotic effects on MCF-7 cells; however, they induced a significant DNA fragmentation in comparison with the nontreated control cells.
Chemical Industries Research Division, National Research Center, Cairo, Egypt. haroun68_2000@yahoo.com
Ahmed A
AA
Haroun
Mona A
MA
Abo-Zeid
Ahmed M
AM
Youssef
Amira
A
Gamal-Eldeen
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002356:Cartilage / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D005260:Female; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D006801:Humans; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D053770:Nanowires / Q000737:chemistry / Q000633:toxicity / Q000648:ultrastructure; D011098:Polyglactin 910 / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity
C009495:titanium dioxide; D011098:Polyglactin 910; D005780:Gelatin; D014025:Titanium