The blood transcriptional signature of chronic hepatitis C virus is consistent with an ongoing interferon-mediated antiviral response.
Blood transcriptional profiling is a powerful tool for understanding global changes after infection, and may be useful for prognosis and prediction of drug treatment responses. This study characterizes the effects of chronic hepatitis C virus (HCV) infection on gene expression by analyzing blood samples from 10 treatment-naïve HCV patients and 6 healthy volunteers. Differential expression analysis of microarray data from peripheral blood mononuclear cells (PBMCs) identified a 136-gene signature, including 66 genes elevated in infected individuals. Most of the upregulated genes were associated with interferon (IFN) activity (including members of the OAS and MX families, ISG15, and IRF7), suggesting an ongoing immune response. This HCV signature was also found to be consistently enriched in many other viral infection and vaccination datasets. These genes were validated using a second cohort composed of 5 HCV patients and 5 healthy volunteers, confirming the upregulation of the IFN signature. In summary, this is the first study to directly compare blood transcriptional profiles from HCV patients with healthy controls. The results show that chronic HCV infection has a pronounced effect on gene expression in PBMCs of infected individuals, and significantly elevates the expression of a subset of IFN-stimulated genes.
Interdepartmental Program in Computational Biology and Bioinformatics, Yale University, New Haven, Connecticut 06511, USA.
Christopher R
CR
Bolen
Michael D
MD
Robek
Leonid
L
Brodsky
Vincent
V
Schulz
Joseph K
JK
Lim
Milton W
MW
Taylor
Steven H
SH
Kleinstein
D000328:Adult; D000998:Antiviral Agents / Q000097:blood / Q000276:immunology / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D016000:Cluster Analysis; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D005260:Female; D016174:Hepacivirus / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D019698:Hepatitis C, Chronic / Q000097:blood / Q000235:genetics* / Q000276:immunology; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D007372:Interferons / Q000097:blood / Q000276:immunology / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D059467:Transcriptome / Q000235:genetics* / Q000276:immunology; D015854:Up-Regulation / Q000235:genetics / Q000276:immunology
D000998:Antiviral Agents; D007372:Interferons