Use of dried blood spots for HIV-1 genotyping in Southeast Asia: Thailand experience.
The multi-region hybridization assay (MHAbce) for genotyping HIV-1 subtypes B, C and circulating recombinant form (CRF01_AE) was evaluated on paired plasma and dried blood spots (DBS) collected from 68 HIV-1 infected individuals in Thailand. CRF01_AE was the predominant subtype identified using plasma samples (51/62) and DBS (24/27). There was no discordance in subtype designations between plasma and DBS.
Department of Retrovirology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. wiriyar@afrims.org
Wiriya
W
Rutvisuttinunt
Miguel A
MA
Arroyo
Vatcharain
V
Assawadarachai
Kultida
K
Poltavee
Francine E
FE
McCutchan
Sodsai
S
Tovanabutra
Gustavo
G
Kijak
Jerome H
JH
Kim
Robert M
RM
Paris
Mark
M
de Souza
D005838:Genotype; D006679:HIV Seropositivity / Q000453:epidemiology / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods*; D012680:Sensitivity and Specificity; D013785:Thailand / Q000453:epidemiology; D019562:Viral Load