A novel micro-linear vector for in vitro and in vivo gene delivery and its application for EBV positive tumors.
BACKGROUND
The gene delivery vector for DNA-based therapy should ensure its transfection efficiency and safety for clinical application. The Micro-Linear vector (MiLV) was developed to improve the limitations of traditional vectors such as viral vectors and plasmids.


METHODS
The MiLV which contained only the gene expression cassette was amplified by polymerase chain reaction (PCR). Its cytotoxicity, transfection efficiency in vitro and in vivo, duration of expression, pro-inflammatory responses and potential application for Epstein-Barr virus (EBV) positive tumors were evaluated.


RESULTS
Transfection efficiency for exogenous genes transferred by MiLV was at least comparable with or even greater than their corresponding plasmids in eukaryotic cell lines. MiLV elevated the expression and prolonged the duration of genes in vitro and in vivo when compared with that of the plasmid. The in vivo pro-inflammatory response of MiLV group was lower than that of the plasmid group. The MEKK1 gene transferred by MiLV significantly elevated the sensitivity of B95-8 cells and transplanted tumor to the treatment of Ganciclovir (GCV) and sodium butyrate (NaB).


CONCLUSIONS
The present study provides a safer, more efficient and stable MiLV gene delivery vector than plasmid. These advantages encourage further development and the preferential use of this novel vector type for clinical gene therapy studies.
Department of Microbial and Biochemical Pharmacy, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China. hswang2000@gmail.com
Hong-Sheng
HS
Wang
Zhuo-Jia
ZJ
Chen
Ge
G
Zhang
Xue-Ling
XL
Ou
Xiang-Ling
XL
Yang
Chris K C
CK
Wong
John P
JP
Giesy
Jun
J
Du
Shou-Yi
SY
Chen
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D045744:Cell Line, Tumor; D016207:Cytokines / Q000097:blood / Q000276:immunology; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics* / Q000276:immunology / Q000627:therapeutic use*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007111:Immunity, Cellular; D048728:MAP Kinase Kinase Kinase 1 / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000235:genetics / Q000628:therapy* / Q000821:virology*; D010957:Plasmids / Q000235:genetics; D014162:Transfection
D016207:Cytokines; D048728:MAP Kinase Kinase Kinase 1