Rapid biochemical synthesis of (11)C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis.
Immuno-PET is a promising approach for improved cancer diagnosis, by taking advantage of the high specificity of antibodies. Here, we present a novel cell-free protein synthesis method for preparing a positron emitter labeled-antibody. Functional anti-human EGFRvIII single chain Fv, MR1-1, was successfully labeled with carbon-11 (half-life=20.4 min) in 5 min (36% yield) by the direct incorporation of the clinical PET tracer, l-[(11)C]methionine. The product [(11)C]MR1-1 was easily and rapidly isolated with high radiochemical purity (>95%) from the reaction solution, by affinity purification. This method would be widely applicable to the preparation of radiolabeled antibodies for PET imaging.
NMR Pipeline Methodology Research Team, RIKEN Systems and Structural Biology Center, Suehiro-cho, Tsurumi, Yokohama, Japan.
Takayoshi
T
Matsuda
Shozo
S
Furumoto
Kae
K
Higuchi
Jun
J
Yokoyama
Ming-Rong
MR
Zhang
Kazuhiko
K
Yanai
Ren
R
Iwata
Takanori
T
Kigawa
D000818:Animals; D002250:Carbon Radioisotopes / Q000737:chemistry; D045744:Cell Line, Tumor; D002474:Cell-Free System; D066246:ErbB Receptors / Q000276:immunology / Q000378:metabolism; D005910:Glioma / Q000000981:diagnostic imaging; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D007553:Isotope Labeling; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D057127:Single-Chain Antibodies / Q000737:chemistry / Q000276:immunology*
C417561:Alexa 488 hydrazide; D002250:Carbon Radioisotopes; D006834:Hydrazines; D019275:Radiopharmaceuticals; D057127:Single-Chain Antibodies; D066246:ErbB Receptors