The expression of thioredoxin-1 in preterm delivery placenta.
Preterm delivery (PTD) is the leading cause of infant mortality and morbidity. However, the mechanism at the molecular level is still unknown. Placental inflammatory response and oxidative stress are associated with PTD. Thioredoxin-1 (TRX-1) regulates oxidative stress, inflammation,  and the activities of transcription factors.


OBJECTIVE
The objective was to detect in placental tissues the expressions of TRX-1 and the TRX-1-related molecules: tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), thioredoxin-1-binding protein-2 (TBP-2),  hypoxia inducible transcription factor 1α (HIF-1α), and forkhead box protein O3A (FoxO3A).


METHODS
PTD was defined as gestation of <37 weeks and term delivery (TD) as ≥37 weeks. The expressions of TRX-1 and TRX-1-related molecules were examined in placental  tissues by real-time polymerase chain rection and western blot.


RESULTS
The expressions of TRX-1, TNF-α, COX-2, HIF-1α, and FoxO3A in the placenta of PTD were significantly higher as compared with TD, but no difference was observed in TBP-2 expression.


CONCLUSIONS
These results indicate that TRX-1 may be adaptively induced by the effects of inflammation and oxidative stress, suggesting protective roles for TRX-1 against these effects in the placenta of PTD.
Kunming University of Science and Technology, Kunming, China.
Junying
J
Song
Xudong
X
Dong
Yan
Y
Chen
Guixian
G
Chen
Hong
H
Liang
Hajime
H
Nakamura
Junji
J
Yodoi
Jie
J
Bai
D000328:Adult; D051546:Cyclooxygenase 2 / Q000235:genetics; D005260:Female; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000235:genetics; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D047928:Premature Birth / Q000378:metabolism*; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D013879:Thioredoxins; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human