Identification of an avian group A rotavirus containing a novel VP4 gene with a close relationship to those of mammalian rotaviruses.
Group A rotaviruses (RVAs) are an important cause of diarrhoeal illness in humans, as well as in mammalian and avian animal species. Previous sequence analyses indicated that avian RVAs are related only distantly to mammalian RVAs. Here, the complete genomes of RVA strain 03V0002E10 from turkey (Meleagris gallopavo) and RVA strain 10V0112H5 from pheasant (Phasianus colchicus) were analysed using a combination of 454 deep sequencing and Sanger sequencing technologies. An adenine-rich insertion similar to that found in the chicken RVA strain 02V0002G3, but considerably shorter, was found in the 3' NCR of the NSP1 gene of the pheasant strain. Most genome segments of both strains were related closely to those of avian RVAs. The novel genotype N10 was assigned to the NSP2 gene of the pheasant RVA, which is related most closely to genotype N6 found in avian RVAs. However, this virus contains a VP4 gene of the novel genotype P[37], which is related most closely to RVAs from pigs, dogs and humans. This strain either may represent an avian/mammalian rotavirus reassortant, or it carries an unusual avian rotavirus VP4 gene, thereby broadening the potential genetic and antigenic variability among RVAs.
Federal Institute for Risk Assessment, Max-Dohrn Straße 8-10, 10589 Berlin, Germany.|Free University Berlin, Takustraße 3, 14195 Berlin, Germany.
Eva
E
Trojnar
Federal Institute for Risk Assessment, Max-Dohrn Straße 8-10, 10589 Berlin, Germany.|Free University Berlin, Takustraße 3, 14195 Berlin, Germany.
Jana
J
Sachsenröder
Institute for Molecular Biology and Bioinformatic, Charite, Arnimallee 22, 14195 Berlin, Germany.
Sven
S
Twardziok
Federal Institute for Risk Assessment, Max-Dohrn Straße 8-10, 10589 Berlin, Germany.
Jochen
J
Reetz
Friedrich Loeffler Institute, Institute for Bacterial Infections and Zoonoses, Naumburger Straße 96a, 07743 Jena, Germany.
Peter H
PH
Otto
Federal Institute for Risk Assessment, Max-Dohrn Straße 8-10, 10589 Berlin, Germany.
Reimar
R
Johne
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001717:Birds; D036022:Capsid Proteins / Q000235:genetics*; D004285:Dogs; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA / Q000379:methods; D013552:Swine; D014421:Turkey
D036022:Capsid Proteins; D012367:RNA, Viral; C060339:VP4 protein, Rotavirus