Intrathecal synthesis in particular types of multiple sclerosis.
OBJECTIVE
The aim of this study was to determine whether there were any differences in intrathecal synthesis of immunoglobulin G (IgG) (IgG index) and number of oligoclonal bands (OCB) among particular types of multiple sclerosis (MS).


METHODS
120 cerebrospinal fluid (CSF) samples were examined from 29 clinically isolated syndrome (CIS) patients and 91 MS patients (77 patients with relapsing-remitting MS (RR), 6 patients with primary progressive course of the disease (PP) and 8 patients in secondary progression (SP)); mean age = 42 years (range = 18 to 70 years). Albumin and IgG in serum and CSF was evaluated using nephelometry; an albumin quotient (CSF albumin/serum albumin), an IgG quotient (CSF IgG/serum IgG) and an IgG index (IgG quotient / albumin quotient) were then calculated. OCB were assessed using isoelectric focusing (IEF) on agarose gel, followed by immunoblotting. All patients were evaluated using the Kurtzke Expanded Disability Status Scale (EDSS).


RESULTS
No statistically significant differences between the IgG index and OC bands relative to particular types of MS were found. Further, there were no significant correlations between EDSS values and intrathecal synthesis (IgG index: QIgG / Qalbumin) and OC bands.


CONCLUSIONS
No difference in intrathecal synthesis (IgG index) and the number of OCB between different types of MS was confirmed.
Department of Neurology, University Hospital Olomouc, Czech Republic.
Vladimira
V
Sladkova
Jan
J
Mares
Petr
P
Hlustik
Jana
J
Langova
Petr
P
Kanovsky
D000293:Adolescent; D000328:Adult; D000368:Aged; D004185:Disability Evaluation; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood* / Q000134:cerebrospinal fluid*; D020528:Multiple Sclerosis, Chronic Progressive / Q000097:blood / Q000134:cerebrospinal fluid; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood / Q000134:cerebrospinal fluid; D043025:Oligoclonal Bands / Q000032:analysis*; D012709:Serum Albumin / Q000378:metabolism; D055815:Young Adult
D007074:Immunoglobulin G; D043025:Oligoclonal Bands; D012709:Serum Albumin