Wound healing of dehiscence defects following different root conditioning modalities: an experimental study in dogs.
OBJECTIVE
The purpose of this study was to investigate the periodontal healing pattern of dehiscence-type defects following different chemical root conditioning modalities.


METHODS
Buccal osseous dehiscence defects were created on six teeth of seven dogs. After dental plaque accumulation, defects were treated with sterile saline solution (control group) or one chemical conditioning modality: citric acid (CA group), ethylenediaminetetraacetic acid (EDTA group), tetracycline (TTC group), citric acid + tetracycline (CA + TTC group), or tetracycline + citric acid (TTC + CA group). After 3 months of healing, clinical parameters were evaluated, and the animals were killed. Histological sections were processed, and a computer-assisted histometric analysis was used to evaluate the formation of new cementum, new bone, and epithelial apical migration.


RESULTS
All treatments yielded significant improvements in terms of probing depth decrease and clinical attachment level gain compared to baseline values; however, without significant differences among the groups (p > 0.05; one-way ANOVA). The highest amount of new cementum was noted in the EDTA group (3.72 ± 0.83 mm, 77.6 %), while the lowest amount of new bone was observed in the TTC group (0.7 ± 0.94 mm, 14.3 %). However, no statistically significant differences could be observed among the groups regarding epithelial apical migration, new cementum, and alveolar bone formation (p > 0.05).


CONCLUSIONS
Chemical root surface conditioning did not promote any significant improvement in periodontal healing pattern of dehiscence-type defects in dogs.


CONCLUSIONS
Chemical root surface conditioning after surgical debridement did not promote positive or negative effects on periodontal healing pattern of dehiscence-type defects.
Department of Diagnosis and Surgery, Araraquara Dental School, São Paulo State University (UNESP), Araraquara, SP, Brazil. danielalzandim@foar.unesp.br
Daniela Leal
DL
Zandim
Fábio Renato Manzolli
FR
Leite
Vanessa Camila
VC
da Silva
Beatriz Maria Valério
BM
Lopes
Luiz Carlos
LC
Spolidorio
José Eduardo Cezar
JE
Sampaio
D016301:Alveolar Bone Loss / Q000188:drug therapy* / Q000601:surgery; D000818:Animals; D025021:Cementogenesis / Q000187:drug effects; D019343:Citric Acid / Q000008:administration & dosage / Q000627:therapeutic use; D019551:Dental Disinfectants / Q000008:administration & dosage / Q000627:therapeutic use; D004285:Dogs; D004338:Drug Combinations; D004492:Edetic Acid / Q000008:administration & dosage / Q000627:therapeutic use; D004846:Epithelial Attachment / Q000187:drug effects; D007091:Image Processing, Computer-Assisted / Q000379:methods; D010012:Osteogenesis / Q000187:drug effects; D017622:Periodontal Attachment Loss / Q000188:drug therapy / Q000601:surgery; D010514:Periodontal Pocket / Q000188:drug therapy / Q000601:surgery; D013357:Subgingival Curettage / Q000379:methods; D013524:Surgical Flaps / Q000601:surgery; D013752:Tetracycline / Q000008:administration & dosage / Q000627:therapeutic use; D014092:Tooth Root / Q000187:drug effects* / Q000601:surgery; D014945:Wound Healing / Q000187:drug effects
D019551:Dental Disinfectants; D004338:Drug Combinations; D019343:Citric Acid; D004492:Edetic Acid; D013752:Tetracycline