[Histological character of intimal hyperplasia post jugular vein grafts and the protective effect of lovastatin].
OBJECTIVE
To investigate the characteristics of intimal hyperplasia and lovastatin's effects on canine jugular venous  prosthesis bypass grafting.


METHODS
In the study, 12 adult mistus dogs were randomly divided into 2 groups: lovastatin group  and control group. All the dogs were performed with jugular venous prosthesis bypass grafting (ePTFE, 6 mm in diameter, and 5 cm  in length). Four weeks later, all the 12 specimens were harvested. The patency and mural thrombus of grafts were evaluated. The  characteristics of intimal hyperplasia were described and measured by HE staining and endothelial nitric oxide synthase (eNOs)  immunohistochemical method. The differences between the two groups were compared.


RESULTS
Four weeks later, 3 grafts with  complete occlusion were found in the two groups separately. Apparent intimal hyperplasia was observed in all the grafts. The  neointima of proximal and distal part in lovastatin group were thinner than in control group respectively (proximal P=0.045, distal  P=0.040). The endothelial cells were found in the surface of neointima. Newly born vessels could be found in the neointima and the  new vessels were more in lovastatin group than in control group (proximal P=0.041, distal P=0.031).


CONCLUSIONS
At the end  of 4 weeks, the intimal hyperplasia with neovascularization was obviously near the anastomosis. Lovastatin showed the ability to  inhibit the intimal hyperplasia and promote the neovascularization.
Department of Interventional and Vascular Surgery, Peking University Third Hospital, Beijing, China.
Guo-jun
GJ
Zhai
Wei-juan
WJ
Yao
Zi-chang
ZC
Jia
Jin-tao
JT
Han
Jun
J
Zhao
Xuan
X
Li
Guo-xiang
GX
Dong
D000714:Anastomosis, Surgical; D000818:Animals; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D020099:Coated Materials, Biocompatible / Q000627:therapeutic use; D004285:Dogs; D005260:Female; D006083:Graft Occlusion, Vascular / Q000473:pathology / Q000517:prevention & control; D006965:Hyperplasia / Q000473:pathology / Q000517:prevention & control; D007601:Jugular Veins / Q000601:surgery*; D008148:Lovastatin / Q000627:therapeutic use*; D008297:Male; D011138:Polytetrafluoroethylene; D017539:Tunica Intima / Q000187:drug effects / Q000473:pathology*
D020099:Coated Materials, Biocompatible; D011138:Polytetrafluoroethylene; D008148:Lovastatin