Deletion of TerD-domain-encoding genes: effect on  Streptomyces coelicolor  development.
TerD-domain-encoding genes (tdd genes) are highly represented in the  Streptomyces coelicolor  genome. One of these, the tdd8 gene, was recently shown to have a crucial influence on growth, differentiation, and spore development of this filamentous bacterium. The investigation of the potential role of tdd genes has been extended here to tdd7 (SCO2367) and tdd13 (SCO4277). Both genes are highly expressed in bacteria grown in liquid-rich medium (tryptic soy broth). However, the deletion of these genes in S. coelicolor showed contrasting effects regarding developmental patterns, sporulation, and antibiotic production. Deletion of the tdd7 gene induced a reduction of growth in liquid medium, wrinkling of the mycelium on solid medium, and poor spore and actinorhodin production. On the other hand, deletion of the tdd13 gene did not significantly affect growth in liquid medium but induced a small colony phenotype on solid medium with abundant sporulation and overproduction of undecylprodigiosin. Although their exact functions remain undefined, the present data suggest a major involvement of TerD proteins in the proper development of S. coelicolor.
Centre d'Étude et de Valorisation de la Diversité Microbienne, Département de biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada.
Édith
É
Sanssouci
Sylvain
S
Lerat
François
F
Daigle
Gilles
G
Grondin
François
F
Shareck
Carole
C
Beaulieu
D000880:Anthraquinones / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000378:metabolism; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D008670:Metals / Q000494:pharmacology; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013171:Spores, Bacterial / Q000502:physiology / Q000648:ultrastructure; D048372:Streptomyces coelicolor / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D000095622:Benzoisochromanequinones
D000880:Anthraquinones; D000900:Anti-Bacterial Agents; D008670:Metals; D011353:Prodigiosin; C047347:undecylprodigiosin; C013390:actinorhodin; D000095622:Benzoisochromanequinones