Mass spectrometry: a revolution in clinical microbiology?
Recently, different bacteriological laboratory interventions that decrease reporting time have been developed. These promising new broad-based techniques have merit, based on their ability to identify rapidly many bacteria, organisms difficult to grow or newly emerging strains, as well as their capacity to track disease transmission. The benefit of rapid reporting of identification and/or resistance of bacteria can greatly impact patient outcomes, with an improvement in the use of antibiotics, in the reduction of the emergence of multidrug resistant bacteria and in mortality rates. Different techniques revolve around mass spectrometry (MS) technology: matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), PCR combined with electrospray ionization-mass spectrometry (PCR/ESIMS), iPLEX MassArray system and other new evolutions combining different techniques. This report emphasizes the (r)evolution of these technologies in clinical microbiology.
Institut National de la Santé et de la Recherche Médicale, U1047 UFR de Médecine, CS83021, 186 Chemin du Carreau de Lanes, 30908, Nîmes Cedex 02, France. jean.philippe.lavigne@chu-nimes.fr
Jean-Philippe
JP
Lavigne
Paula
P
Espinal
Catherine
C
Dunyach-Remy
Nourredine
N
Messad
Alix
A
Pantel
Albert
A
Sotto
D001431:Bacteriological Techniques / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008829:Microbiology / Q000295:instrumentation*