In vitro morphological bud formation in organ-like three-dimensional structure from mouse ES cells induced by FGF10 signaling.
Embryonic stem (ES) cells have a pluripotent ability to differentiate into a variety of cell lineages in vitro. Using an embryoid body (EB) culture system, we developed a gut-like three-dimensional structure from mouse ES cells (the ES 3-D structure). Genetic studies implicate fibroblast growth factor 10 (FGF10)-FGF receptor 2b (FGFR2b) signaling as a critical regulator of lung bud morphogenesis in the embryonic foregut. The aim of the present study was to form a putative respiratory tract in the ES 3-D structure. By local application of FGF10 protein, we successfully demonstrated in vitro morphological formation of putative primitive respiratory tract-like processes, or buds, in the ES 3-D structure. Such organs that are differentiated from ES cells may provide new insights into tissue engineering and regenerative medicine.
Department of Physiology; School of Medicine; University of Occupational and Environmental Health; Kitakyushu, Japan ; Department of Otorhinolaryngology; School of Medicine; University of Occupational and Environmental Health, Kitakyushu, Japan.
Toyoaki
T
Ohbuchi
Miyako
M
Takaki
Hiromi
H
Misawa
Hideaki
H
Suzuki
Yoichi
Y
Ueta