Detection of loci for allergic asthma using SMXA recombinant inbred strains of mice.
Asthma is regarded as a multifactorial inflammatory disorder arising as a result of inappropriate immune responses in genetically susceptible individuals to common environmental antigens. However, the precise molecular basis is unknown. To identify genes for susceptibility to three asthma-related traits, airway hyperresponsiveness (AHR), eosinophil infiltration, and allergen-specific serum IgE levels, we conducted a genetic analysis using SMXA recombinant inbred (RI) strains of mice. Quantitative trait locus analysis detected a significant locus for AHR on chromosome 17. For eosinophil infiltration, significant loci were detected on chromosomes 9 and 16. Although we could not detect any significant loci for allergen-specific serum IgE, analysis of consomic strains showed that chromosomes 17 and 19 carried genes that affected this trait. We detected genetic susceptibility loci that separately regulated the three asthma-related phenotypes. Our results suggested that different genetic mechanisms regulate these asthma-related phenotypes. Genetic analyses using murine RI and consomic strains enhance understanding of the molecular mechanisms of asthma in human.
Division of Experimental Animals, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Tamio
T
Ohno
Masakazu
M
Okamoto
Toru
T
Hara
Naozumi
N
Hashimoto
Kazuyoshi
K
Imaizumi
Miyoko
M
Matsushima
Masahiko
M
Nishimura
Kaoru
K
Shimokata
Yoshinori
Y
Hasegawa
Tsutomu
T
Kawabe
D000818:Animals; D001249:Asthma / Q000235:genetics*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002874:Chromosome Mapping; D004804:Eosinophils / Q000276:immunology; D020022:Genetic Predisposition to Disease; D005838:Genotype; D007073:Immunoglobulin E / Q000097:blood; D051379:Mice; D010047:Ovalbumin; D040641:Quantitative Trait Loci
D007073:Immunoglobulin E; D010047:Ovalbumin