In aggressive forms of mastocytosis, TET2 loss cooperates with c-KITD816V to transform mast cells.
Although a role for oncogenic KIT in driving mast cell disease is clear, the mechanisms driving the multiple phenotypic and clinical manifestations of this disorder are not well elucidated. We now show, using a large cohort of mastocytosis patients, including an almost equal number of aggressive and nonaggressive cases of systemic mastocytosis, that in contrast to the oncogenic KITD816V, TET2 mutation statistically associates with aggressive forms of the disease. By infecting primary murine bone marrow-derived mast cells with KITD816V, we also observe a significant and competitive growth advantage for KITD816V in Tet2-nullizygous compared with wild-type cells. TET2-deficient cells display increased proliferation and can survive in the absence of cytokines. Taken together, these data demonstrate a oncogenic cooperation in mast cells and reveal TET2 mutation as a potential marker to diagnose and predict severe forms of mastocytosis.
Inserm U1068, Centre de Recherche en Cancérologie de Marseille, Centre de Référence des Mastocytoses, Institut Paoli-Calmettes, Université de la Méditerranée, Marseille, France.
Erinn
E
Soucie
Katia
K
Hanssens
Thomas
T
Mercher
Sophie
S
Georgin-Lavialle
Gandhi
G
Damaj
Cristina
C
Livideanu
Maria Olivia
MO
Chandesris
Yolène
Y
Acin
Sebastien
S
Létard
Paulo
P
de Sepulveda
Olivier
O
Hermine
Olivier A
OA
Bernard
Patrice
P
Dubreuil
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019943:Amino Acid Substitution; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D002470:Cell Survival / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002478:Cells, Cultured; D015331:Cohort Studies; D004268:DNA-Binding Proteins / Q000235:genetics*; D049308:Dioxygenases; D005260:Female; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000378:metabolism* / Q000473:pathology; D008415:Mastocytosis / Q000235:genetics* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D008875:Middle Aged; D009154:Mutation; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D013997:Time Factors; D014162:Transfection
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D049308:Dioxygenases; C541081:TET2 protein, human; C551914:Tet2 protein, mouse; D019009:Proto-Oncogene Proteins c-kit