Purification of a novel anticancer peptide from enzymatic hydrolysate of Mytilus coruscus.
We applied enzymatic hydrolysis and tangential flow filtration (TFF) to purify a novel anticancer peptide from Mytilus coruscus (M. coruscus) and investigated its anticancer properties. To prepare the peptide, eight proteases were employed for enzymatic hydrolysis. Pepsin hydrolysates, which showed clearly superior cytotoxic activity on prostate cancer cells, were further purified using a flow filtration system using a TFF and consecutive chromatographic methods. Finally, a novel anticancer peptide was obtained, and the sequence was identified as Ala-Phe-Asn-Ile-His- Asn-Arg-Asn-Leu-Leu. The peptide from M. coruscus effectively induced cell death on prostate, breast and lung cancer cells but not on normal liver cells. This is the first report of an anticancer peptide derived from the hydrolysates of M. coruscus.
Division of Food Bioscience, Konkuk University, Chungju 380-701, Korea, Korea Nokyong Research Center, Konkuk University, Chungju 380-701, Korea.
Eun-Kyung
EK
Kim
Hong-Joo
HJ
Joung
Yon-Suk
YS
Kim
Jin-Woo
JW
Hwang
Chang-Bum
CB
Ahn
You-Jin
YJ
Jeon
Sang-Ho
SH
Moon
Pyo-Jam
PJ
Park
D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017209:Apoptosis; D001943:Breast Neoplasms / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D002852:Chromatography, Ion Exchange / Q000379:methods; D005260:Female; D006801:Humans; D006868:Hydrolysis; D008297:Male; D049878:Mytilus / Q000737:chemistry / Q000201:enzymology*; D010434:Pepsin A / Q000737:chemistry; D010447:Peptide Hydrolases / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000473:pathology; D011492:Protein Hydrolysates / Q000737:chemistry / Q000494:pharmacology*
D000970:Antineoplastic Agents; D011492:Protein Hydrolysates; D010447:Peptide Hydrolases; D010434:Pepsin A