Hypoxia-induced EMAP-II transcription in colorectal cancer.
Endothelial monocyte-activating polypeptide-II (p431EMAP-II) is a proinflammatory cytokine and a chemoattractant for mononuclear phagocytes and polymorphonuclear leucocytes, found in culture supernatants of many tumour cell lines. It was demonstrated that p43/EMAP-II induces apoptosis in mitogen-stimulated lymphocytes, and suggested that it may be a constituent of a novel immune evasion mechanism employed by tumour cells. Quantitative real-time reverse transcription- polymerase chain reaction (qRT-PCR) analysis for EMAP-II mRNA was performed for colorectal adenocarcinoma cell lines, DLD-1, HT 29; human umbilical vein endothelial cells (HUVEC); and normal colon under normal and hypoxic conditions. Under hypoxic conditions, EMAP-II transcript expression increased up to 22-fold over normoxia in tumour cells, while there was 1-fold increase due to hypoxia in HUVEC and no increase in normal colon. These results demonstrate that EMAP-II transcripts are upregulated in tumour cells in hypoxic conditions and support the notion that EMAP-II plays a complex and important role in human cancer.
Clinical Pathology Department, Faculty of Medicine, Minia University, Egypt.
Manal M S
MM
Youssef
Yee M
YM
Heng
Desmond G
DG
Powe
Jodie
J
Edgson
I O
IO
Ellis
Clifford
C
Murray
D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D015687:Cell Hypoxia / Q000502:physiology; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D019073:HT29 Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D008934:Mitogens / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic; D015854:Up-Regulation
C485471:EML2 protein, human; D008869:Microtubule-Associated Proteins; D008934:Mitogens; D012333:RNA, Messenger