[Effect of hOGG1 gene on the oxidative damage of mitochondrial DNA induced by hexavalent chromium].
OBJECTIVE
To explore the effect of hOGG1 gene on the repair capability of oxidative damage in mitochondrial DNA induced by hexavalent chromium (Cr (VI)).


METHODS
After incubating with Cr(VI) at the concentrations of 2, 8, 32 micromol/L for 24 hours, mitochondria from L-02 hepatocytes were collected. Cellular ROS and hOGG1 mRNA and mitochondrial 8-OHdG and hOGG1 protein in L-02 hepatocytes were examined by Fluorometric Assay Kit, RT-qPCR, 8-OHdG ELISA Kit and western blot respectively.


RESULTS
Compared with the control group, the levels of cellular ROS and mitochondrial 8-OHdG in 8 and 32 micromol/L Cr(VI) treated groups were significantly increased (P < 0.05). The levels of cellular hOGG1 mRNA and mitochondrial hOGG1 protein in 2 micromol/L Cr (VI) treated group were increased significantly (P < 0.05), but decreased in 32 micromol/l. group (P < 0.05).


CONCLUSIONS
Overproduction of cellular ROS could be induced by Cr (VI) and could result in genomic DNA oxidative damage, and the lower expression of cellular hOGG1 gene could decrease the repair capability of mitochondrial DNA. Therefore, the cellular hOGG1 gene exerted important effect in the repair of mitochondrial DNA oxidative damage induced by Cr( VI).
Department of Health Toxicology, School of Public Health, Central South University, Changsha 410078, China. lp2501040220@163.com
Peng
P
Li
Caigao
C
Zhong
An
A
Wang
Lan
L
Guan
Fang
F
Xiao
Yue
Y
Zou
Yuan
Y
Yang
D000080242:8-Hydroxy-2'-Deoxyguanosine; D002857:Chromium / Q000633:toxicity*; D004249:DNA Damage; D045647:DNA Glycosylases / Q000187:drug effects* / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000187:drug effects*; D003849:Deoxyguanosine / Q000031:analogs & derivatives; D022781:Hepatocytes; D006801:Humans; D008928:Mitochondria
D004272:DNA, Mitochondrial; D002857:Chromium; C074702:chromium hexavalent ion; D000080242:8-Hydroxy-2'-Deoxyguanosine; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; D003849:Deoxyguanosine