Nitrogen starvation induces expression of Lg-FLO1 and flocculation in bottom-fermenting yeast.
When exponentially growing cells of bottom-fermenting yeast were starved for nitrogen or were grown on proline (a non-preferred nitrogen source), flocculation was induced. This flocculation was not induced by starvation for either carbon or amino acids. Expression of Lg-FLO1, which is required for flocculation of bottom-fermenting yeast, was also found to be induced by starvation for nitrogen. This suggests that the flocculation of bottom-fermenting yeast is under the control of a nitrogen catabolite repression (NCR)-like mechanism.
Research Laboratories for Brewing, Asahi Breweries Ltd, Ibaraki, Japan. tomoo.ogata@asahibeer.co.jp
Tomoo
T
Ogata
D002448:Cell Adhesion; D005285:Fermentation; D005427:Flocculation; D015966:Gene Expression Regulation, Fungal; D037241:Mannose-Binding Lectins / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis*; D013312:Stress, Physiological
C087578:FLO1 protein, S cerevisiae; D037241:Mannose-Binding Lectins; D029701:Saccharomyces cerevisiae Proteins; D011392:Proline; D009584:Nitrogen