Tsp66E, the Drosophila KAI1 homologue, and Tsp74F function to regulate ovarian follicle cell and wing development by stabilizing integrin localization.
The metastasis suppressor KAI1/CD82 has been implicated in various cellular processes; however, its function in development is not fully understood. Here, we generated and characterized mutants of Tsp66E and Tsp74F, which are Drosophila homologues of KAI1/CD82 and Tspan11, respectively. These mutants exhibited egg elongation defects along with disturbed integrin localization and actin polarity. Moreover, the defects were enhanced by mutation of inflated, an αPS2 integrin gene. Mutant ovaries had elevated αPS2 integrin levels and reduced endocytic trafficking. These results suggest that Drosophila KAI1/CD82 affects the polarized localization and the level of integrin, which may contribute to epithelial cell polarity.
Department of Biological Sciences, Konkuk University, Seoul 143-701, Republic of Korea.
Seung Yeop
SY
Han
Minjung
M
Lee
Yoon Ki
YK
Hong
Soojin
S
Hwang
Gahee
G
Choi
Yoon Seok
YS
Suh
Seung Hwan
SH
Park
Soojin
S
Lee
Sang-Hee
SH
Lee
Jongkyeong
J
Chung
Sung Hee
SH
Baek
Kyoung Sang
KS
Cho
D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D003911:Dextrans / Q000378:metabolism / Q000493:pharmacokinetics; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D004331:Drosophila melanogaster / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D004705:Endocytosis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D039001:Integrin alpha Chains / Q000235:genetics / Q000502:physiology*; D016023:Integrins / Q000378:metabolism*; D051861:Kangai-1 Protein / Q000235:genetics / Q000502:physiology; D008297:Male; D018613:Microscopy, Confocal; D009154:Mutation; D006080:Ovarian Follicle / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D010063:Ovum / Q000254:growth & development / Q000378:metabolism; D059470:Tetraspanins / Q000235:genetics / Q000502:physiology*; D014921:Wings, Animal / Q000254:growth & development* / Q000378:metabolism
D000199:Actins; D003911:Dextrans; D029721:Drosophila Proteins; D039001:Integrin alpha Chains; D016023:Integrins; D051861:Kangai-1 Protein; D059470:Tetraspanins; C577976:Tsp66E protein, Drosophila; C577977:Tsp74F protein, Drosophila; C082188:if protein, Drosophila