Chemico-physical characterisation and in vivo biocompatibility assessment of DLC-coated coronary stents.
The vast majority of stent thrombosis occurs in the acute and sub-acute phases and is more common in patients with acute coronary syndromes, due to the thrombotic milieu where stent struts are positioned. Stent thrombosis is likely due to incomplete tissue coverage of metallic stents as the contact between metallic stents and blood elements may lead to platelet adhesion and trigger vessel thrombosis. If a stent is covered after 7 days, the risk that it will be found uncovered at later stages is very low (<1%). In this article, we demonstrate that diamond-like carbon (DLC) coatings, deposited by physical vapour deposition, promote rapid endothelisation of coronary stent devices, with very low platelets activation, reducing thrombotic clots. We relate these behaviours to the surface and bulk material properties of the DLC films, subjected to a comprehensive chemico-physical characterisation using several techniques (X-ray photoelectron spectroscopy, atomic force microscopy, field-emission scanning electron microscope, transmission electron microscopy combined with electron energy loss spectroscopy, Raman and dispersive X-ray spectroscopy). In vivo studies, conducted on 24 pigs, have shown complete endothelisation after 7 days, with no fibrin mesh and with only rare monocytes scattered on the endothelial layer while 30 and 180 days tests have shown reduced inflammatory activation and a complete stabilisation of the vessel healing, with a minimal neointimal proliferation. The integral and permanent DLC film coating improves haemo- and bio-compatibility and leads to an excellent early vessel healing of the stent whilst the extremely thin strut thickness reduces the amount of late neointima and consequently the risk of late restenosis. These data should translate into a reduced acute and sub-acute stent thrombosis.
Applied Science and Technology Department (DISAT), Polytechnic of Turin, Corso Duca degli Abruzzi 24, 10129 Torino, Italy. micaela.castellino@polito.it
Micaela
M
Castellino
Vlad
V
Stolojan
Alessandro
A
Virga
Massimo
M
Rovere
Karine
K
Cabiale
Marco R
MR
Galloni
Alberto
A
Tagliaferro
D054058:Acute Coronary Syndrome / Q000378:metabolism; D000327:Adsorption; D000418:Albumins / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001792:Blood Platelets / Q000378:metabolism; D002244:Carbon / Q000737:chemistry; D049109:Cell Proliferation; D002627:Chemistry, Physical / Q000379:methods*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004583:Electrons; D042783:Endothelial Cells / Q000378:metabolism; D005340:Fibrinogen / Q000737:chemistry; D007249:Inflammation; D018625:Microscopy, Atomic Force / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D046529:Microscopy, Electron, Transmission / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods; D015607:Stents; D013499:Surface Properties; D013552:Swine; D013927:Thrombosis; D013997:Time Factors
D000418:Albumins; D001672:Biocompatible Materials; D020099:Coated Materials, Biocompatible; D002244:Carbon; D005340:Fibrinogen