Visualization of the intracellular location and stability of DNA origami with a label-free fluorescent probe.
We report a label-free fluorescent strategy to study the distribution and stability of DNA origami nanostructures in live, cellular systems, using carbazole-based biscyanine as a probe molecule which has the characteristic property of restriction of intramolecular rotation (RIR) induced emission.
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China.
Xibo
X
Shen
Qiao
Q
Jiang
Jinye
J
Wang
Luru
L
Dai
Guozhang
G
Zou
Zhen-Gang
ZG
Wang
Wei-Qiang
WQ
Chen
Wei
W
Jiang
Baoquan
B
Ding
D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry
D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3; C085321:cyanine dye 5; D004247:DNA