Molecular analysis of fungal gene expression upon interkingdom competition with insects.
Fungi produce an astonishing variety of secondary metabolites, some of which belong to the most toxic compounds in the living world. However, the benefits of secondary metabolites for fungi are often obscure. Here we describe a method that will aid in the analysis of fungal gene expression upon interkingdom competition with insects. It allows for isolation of fungal RNA from surface-grown mycelium after confrontation with insect larvae, and subsequent molecular analysis.
Abteilung für Botanische Genetik und Molekularbiologie, Botanisches Institut und Botanischer Garten, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
Ulrike
U
Fohgrub
Frank
F
Kempken
D000818:Animals; D001233:Aspergillus nidulans / Q000235:genetics* / Q000502:physiology*; D004331:Drosophila melanogaster / Q000502:physiology*; D020869:Gene Expression Profiling / Q000379:methods*; D015966:Gene Expression Regulation, Fungal; D007814:Larva / Q000502:physiology; D012331:RNA, Fungal / Q000235:genetics / Q000302:isolation & purification
D012331:RNA, Fungal