Mouse Clr-g, a ligand for NK cell activation receptor NKR-P1F: crystal structure and biophysical properties.
Interactions between C-type lectin-like NK cell receptors and their protein ligands form one of the key recognition mechanisms of the innate immune system that is involved in the elimination of cells that have been malignantly transformed, virally infected, or stressed by chemotherapy or other factors. We determined an x-ray structure for the extracellular domain of mouse C-type lectin related (Clr) protein g, a ligand for the activation receptor NKR-P1F. Clr-g forms dimers in the crystal structure resembling those of human CD69. This newly reported structure, together with the previously determined structure of mouse receptor NKR-P1A, allowed the modeling and calculations of electrostatic profiles for other closely related receptors and ligands. Despite the high similarity among Clr-g, Clr-b, and human CD69, these molecules have fundamentally different electrostatics, with distinct polarization of Clr-g. The electrostatic profile of NKR-P1F is complementary to that of Clr-g, which suggests a plausible interaction mechanism based on contacts between surface sites of opposite potential.
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, vvi, 16206 Praha 6, Czech Republic. t.skalova@gmail.com
Tereza
T
Skálová
Kristýna
K
Kotýnková
Jarmila
J
Dušková
Jindřich
J
Hašek
Tomáš
T
Koval
Petr
P
Kolenko
Petr
P
Novák
Petr
P
Man
Pavel
P
Hanč
Ondřej
O
Vaněk
Karel
K
Bezouška
Jan
J
Dohnálek
D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry / Q000276:immunology; D018360:Crystallography, X-Ray; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry* / Q000276:immunology; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D017434:Protein Structure, Tertiary; D011971:Receptors, Immunologic / Q000737:chemistry* / Q000276:immunology; D055672:Static Electricity; D040681:Structural Homology, Protein; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D008024:Ligands; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D000099192:CD69 Antigens; C470572:Dcl1 protein, mouse; C442138:Nkrp1f protein, mouse