In Situ probing of newly synthesized peptidoglycan in live bacteria with fluorescent D-amino acids.
Tracking a bug's life: Peptidoglycan (PG) of diverse bacteria is labeled by exploiting the tolerance of cells for incorporating different non-natural D-amino acids. These nontoxic D-amino acids preferably label the sites of active PG synthesis, thereby enabling fine spatiotemporal tracking of cell-wall dynamics in phylogenetically and morphologically diverse bacteria. HCC = 7-hydroxycoumarin, NBD = 7-nitrobenzofurazan, TAMRA = carboxytetramethylrhodamine.
Indiana University, Bloomington, IN 47405, USA.
Erkin
E
Kuru
H Velocity
HV
Hughes
Pamela J
PJ
Brown
Edward
E
Hall
Srinivas
S
Tekkam
Felipe
F
Cava
Miguel A
MA
de Pedro
Yves V
YV
Brun
Michael S
MS
VanNieuwenhze
D016960:Agrobacterium tumefaciens / Q000378:metabolism; D000596:Amino Acids / Q000737:chemistry*; D001412:Bacillus subtilis / Q000378:metabolism; D001419:Bacteria / Q000378:metabolism*; D001583:Benzoxazoles / Q000737:chemistry; D015374:Biosensing Techniques; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D003374:Coumarins / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D008853:Microscopy; D010457:Peptidoglycan / Q000096:biosynthesis* / Q000737:chemistry
D000596:Amino Acids; D001583:Benzoxazoles; D003374:Coumarins; D005456:Fluorescent Dyes; D010457:Peptidoglycan; C020160:benzofurazan; C030123:coumarin