An experimental model of contact dermatitis: evaluation of the oxidative profile of Wistar rats treated with free and nanoencapsulated clobetasol.
OBJECTIVE
An experimental animal model of contact dermatitis (CD) was used to investigate the effects of free and nanoencapsulated clobetasol propionate on the skin and on the oxidative profile of liver tissue.


METHODS
Female Wistar rats were divided into  six groups, each containing eight rats. The first group, control (C), was sensitized with solid vaseline. Group 2, (CD), was sensitized with 5% NiSO(4). Groups 3 and 4 were sensitized with 5% NiSO(4) and treated with free (FC) and nanoencapsulated (NC) clobetasol (0.42 mg/g),  respectively, daily for 5 days. Group 5 was treated with nanoencapsulated clobetasol (0.42 mg/g) on days 1, 3, and 5 (C135) and group 6 received a hydrogel containing empty nanoparticles (NP) daily for 5 days. Thiobarbituric acid reactive substances (TBARS), carbonyl levels, non-protein  sulfhydryl groups (NPSH) and catalase activity were measured in liver homogenates.


RESULTS
A significant increase was observed in the levels of TBARS, NPSH, and catalase activity for the groups CD and NP.


CONCLUSIONS
Our results suggest that both NiSO(4)  sensitization and NP administration induced oxidation of cellular lipids and activated the antioxidant enzyme catalase to protect from this damage. These results also indicated that daily treatment with the free and nanoencapsulated clobetasol, as well as treatment with the nanoencapsulated  clobetasol every other day, were able to prevent these redox alterations and protect against histological damage.
Departamento de Química, Laboratório de Enzimologia Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, Santa Maria, RS, Brasil.
Jeandre Augusto dos Santos
JA
Jaques
João Felipe Peres
JF
Rezer
Jader Betsch
JB
Ruchel
Viviane do Carmo Gonçalves
Vdo C
Souza
Kelly de Vargas
Kde V
Pinheiro
Karine Bizzi
KB
Schlemmer
Josiane Bizzi
JB
Schlemmer
Tatiana Montagner Dalcin
TM
Bertoldo
Nara Maria Beck
NM
Martins
Cláudia de Mello
Cde M
Bertoncheli
Márcia Camponogara
MC
Fontana
Ruy Carlos Ruver
RC
Beck
Daniela Bitencourt Rosa
DB
Leal
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002990:Clobetasol / Q000008:administration & dosage* / Q000627:therapeutic use*; D003877:Dermatitis, Contact / Q000188:drug therapy* / Q000378:metabolism; D004337:Drug Carriers / Q000008:administration & dosage / Q000737:chemistry*; D005260:Female; D015227:Lipid Peroxidation / Q000187:drug effects; D049329:Nanostructures / Q000008:administration & dosage / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D004337:Drug Carriers; D002990:Clobetasol; D002374:Catalase; D013482:Superoxide Dismutase