The effect of antimicrobial photodynamic therapy on human ciliated respiratory mucosa.
BACKGROUND
Chronic recurrent sinusitis (CRS) is one of the most common chronic conditions in the United States. There is a significant subpopulation of CRS patients who remain resistant to cure despite rigorous treatment regimens including surgery, allergy therapy, and prolonged antibiotic therapy. Antimicrobial photodynamic therapy (aPDT) is a noninvasive nonantibiotic broad spectrum antimicrobial treatment. Our previous in vitro studies demonstrated that aPDT reduced CRS polymicrobial biofilm and planktonic bacteria and fungi by > 99.9% after a single treatment. Prior to human treatment however, aPDT treatment must be demonstrated to not result in histologic damage to the sinus ciliated respiratory epithelium. The objective of this study was to demonstrate the safety of aPDT treatment on a living human ciliated respiratory mucosal model (EpiAirway).


METHODS
A study of aPDT treatment of EpiAirway was performed. Treatment groups included a nontreatment control, laser light alone, photosensitizer alone, and therapeutic photosensitizer and light combination (aPDT). At completion of treatment, the EpiAirway tissue was fixed in 10% formalin, paraffin-embedded, sectioned, H&E stained and mounted. All samples were blinded and microscopically examined by a human pathologist to assess any effect of aPDT on the tissue, cilia, or mucosal glands. The results were correlated with the treatment parameters.


RESULTS
The EpiAirway histologic study demonstrated no histologic alteration of the respiratory cilia or mucosal epithelium in any of the treatment groups.


CONCLUSIONS
aPDT is a safe treatment for CRS resulting in no histologic alteration of human ciliated respiratory mucosa as is found in the human sinuses.
PhotoBiologix, Inc., Minneapolis, Minnesota, USA. bielx001@umn.edu
Merrill A
MA
Biel
John W
JW
Jones
Lisa
L
Pedigo
Aaron
A
Gibbs
Nicolas
N
Loebel
D000890:Anti-Infective Agents / Q000627:therapeutic use*; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development*; D018441:Biofilms; D002923:Cilia / Q000187:drug effects / Q000382:microbiology / Q000473:pathology*; D006801:Humans; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use; D010933:Plankton; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000382:microbiology / Q000473:pathology
D000890:Anti-Infective Agents; D017319:Photosensitizing Agents