Real-time sequencing to decipher the molecular mechanism of resistance of a clinical pan-drug-resistant Acinetobacter baumannii isolate from Marseille, France.
We compare the whole-genome sequences of two multidrug-resistant clinical Acinetobacter baumannii isolates recovered in the same patient before (ABIsac_ColiS susceptible to colistin and rifampin only) and after (ABIsac_ColiR resistant to colistin and rifampin) treatment with colistin and rifampin. We decipher all the molecular mechanisms of antibiotic resistance, and we found mutations in the rpoB gene and in the PmrAB two-component system explaining resistance to rifampin and colistin in ABIsac_ColiR, respectively.
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergents (URMITE), UMR CNRS-IRD-INSERM, IHU Méditerranée Infection, Aix-Marseille Université, Marseille, France.
Jean-Marc
JM
Rolain
Seydina M
SM
Diene
Marie
M
Kempf
Gregory
G
Gimenez
Catherine
C
Robert
Didier
D
Raoult
D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D002876:Chromosomes, Bacterial; D003091:Colistin / Q000494:pharmacology / Q000627:therapeutic use*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005602:France; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D012293:Rifampin / Q000494:pharmacology / Q000627:therapeutic use*; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C409039:PmrB protein, bacteria; D014157:Transcription Factors; C081902:pmrA protein, Bacteria; D012293:Rifampin; D003091:Colistin