Bos grunniens papillomavirus type 1: a novel deltapapillomavirus associated with fibropapilloma in yak.
Papillomaviruses (PVs) have been widely identified among vertebrates, but have not yet been reported to infect yaks. We report, for the first time, a novel deltapapillomavirus that was associated with fibropapilloma in yak herds on the Qinghai-Tibetan Plateau. Six skin papilloma samples were collected and examined using histopathology, immunohistochemistry and PCR assays. The samples were identified as fibropapilloma and were found to contain PV antigens. Sequencing of diagnostic PCR products and the full-length genome revealed that all samples were infected with the same PV type. The whole virus genome was 7946 bp in length and possessed the common PV genomic organization. The virus was identified as a novel PV type and designated Bos grunniens papillomavirus type 1 (BgPV-1) based on the nucleotide sequence alignment of the L1 ORF. It is classified in the Delta-4 species of the genus Deltapapillomavirus based on phylogenetic analysis of the L1 ORF. Identification of this novel PV type provides further information about the pathology, development of diagnostic methods and evolutionary studies of the family Papillomaviridae.
Department of Veterinary Microbiology, University of Miyazaki, Miyazaki, Japan.|Japan Society for the Promotion of Science, Tokyo, Japan.
Wei
W
Zhu
Department of Veterinary Microbiology, University of Miyazaki, Miyazaki, Japan.|Japan Society for the Promotion of Science, Tokyo, Japan.|The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
Jian-Bao
JB
Dong
Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, PR China.
Jun
J
Zhang
Department of Veterinary Pathology, University of Tokyo, Tokyo, Japan.
Kazuyuki
K
Uchida
Department of Veterinary Pathology, University of Tokyo, Tokyo, Japan.
Ken-Ichi
KI
Watanabe
Department of Veterinary Microbiology, University of Miyazaki, Miyazaki, Japan.
Yoshitaka
Y
Goto
Division of Infection Control and Disease Prevention, Department of Veterinary Medical Science, University of Tokyo, Tokyo, Japan.
Takeshi
T
Haga
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D002417:Cattle; D052687:Deltapapillomavirus / Q000145:classification* / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010212:Papilloma / Q000276:immunology / Q000662:veterinary / Q000821:virology*; D030361:Papillomavirus Infections / Q000276:immunology / Q000662:veterinary / Q000821:virology*; D010802:Phylogeny; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA
D000956:Antigens, Viral