Stability improvement of immobilized lactoperoxidase using polyaniline polymer.
Enzyme engineering via immobilization techniques is perfectly compatible against the other chemical or biological approximate to improve enzyme functions and stability. In this study lactoperoxidase was immobilized onto polyaniline polymer activated with glutaraldehyde as a bifunctional agent, to improve enzyme properties. Polyaniline polymer was used due its unique physical and chemical properties to immobilize lactoperoxidase (LPO). The optimum activity of immobilized LPO was observed at pH 6 and 55 °C, which has been increased about 10 °C for the immobilized enzyme. The immobilized enzyme maintained absolutely active for 60 days whereas the native enzyme lost 80 % of its initial activity within this period of time. Moreover, the immobilized enzyme can be reused for several times without loss of activity. The kinetic parameter studies showed slight differences between free and immobilized enzymes. The K(m) and K(m.app) were calculated to be 0.6 and 0.4; also V(max) and V(max.app) were 1.3 and 0.9 respectively.
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
Fariba
F
Jafary
Soheila
S
Kashanian
Ziadin Samsam
ZS
Sharieat
Farzaneh
F
Jafary
Kobra
K
Omidfar
Maliheh
M
Paknejad
D000814:Aniline Compounds / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability / Q000187:drug effects; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D007700:Kinetics; D007784:Lactoperoxidase / Q000378:metabolism*; D009930:Organic Chemicals / Q000494:pharmacology; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D059027:Recycling; D012997:Solvents / Q000494:pharmacology; D013696:Temperature
D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D009930:Organic Chemicals; D011108:Polymers; D012997:Solvents; C416807:polyaniline; D007784:Lactoperoxidase