Molecular and parasitological detection of Leishmania spp. in dogs caught in Palmas, TO, Brazil.
This study evaluated occurrences of Leishmania infantum in dogs in the municipality of Palmas, Tocantins, comparing diagnostic data obtained using the polymerase chain reaction (PCR) and parasitological diagnosis. Blood samples and lymph node aspirates were collected from 63 dogs of males and females and various ages and races, with or without owners, between August 2009 and June 2010. Slides containing smears of lymph node aspirates were stained with Giemsa stained. In PCR, the 145 bp target sequence of the LT1 fragment, located in the Leishmania donovani kDNA minicircle was detected using the RV1 and RV2 oligonucleotide primers. The chi-square test revealed that there was a significant relationship between the symptoms and dogs that were positive for visceral leishmaniasis (VL). The parasitological investigation showed concordance of 66.7% with PCR on blood and 84.1% with PCR on lymph node aspirate. In addition to these tests, evaluations of the diagnoses in parallel and in series were conducted, which showed concordances with the parasitological test of 76.2% and 74.6%, respectively. The results make it possible to suggest that PCR on lymph nodes should be used in evaluating large populations (surveys) and that the parasitological test should be used for initial clinical evaluations in veterinary consultation offices.
Laboratório de Biotecnologia, Universidade Federal do Tocantins, Palmas, TO, Brasil.
Natália Melquie Monteiro
NM
Teles
Maria Augusta Paes
MA
Agostini
Júlio Gomes
JG
Bigeli
Rosalba Valadares
RV
Noleto
Jaqueline Dias
JD
Oliveira
Waldesse Piragé
WP
de Oliveira Junior
D000818:Animals; D001938:Brazil; D004285:Dogs; D005260:Female; D007891:Leishmania / Q000302:isolation & purification*; D007896:Leishmaniasis / Q000175:diagnosis / Q000662:veterinary*; D008198:Lymph Nodes / Q000469:parasitology; D008297:Male; D016133:Polymerase Chain Reaction