Laser cutting eliminates nucleic acid cross-contamination in dried-blood-spot processing.
Dried blood spots (DBS) are useful for molecular assays but are prone to false positives from cross-contamination. In our malaria DBS assay, cross-contamination was encountered despite cleaning techniques suitable for HIV-1. We therefore developed a contact-free laser cutting system that effectively eliminated cross-contamination during DBS processing.
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington, USA. murphysc@uw.edu
Sean C
SC
Murphy
Glenda
G
Daza
Ming
M
Chang
Robert
R
Coombs
D001769:Blood / Q000469:parasitology; D019411:Clinical Laboratory Techniques / Q000379:methods*; D059018:DNA Contamination; D003951:Diagnostic Errors; D006801:Humans; D007834:Lasers; D008288:Malaria / Q000175:diagnosis; D009696:Nucleic Acids / Q000302:isolation & purification; D013048:Specimen Handling / Q000379:methods*
D009696:Nucleic Acids