Detection of Streptococcus pyogenes virulence factors by multiplex PCR.
Streptococcus pyogenes (GAS) is a human pathogen that causes multiple infections worldwide. The pathogenic properties of GAS strains are often linked to the production of virulence factors such as toxins, proteases or DNases. Detection of virulence factors produced by GAS strains can be used to either determine pathogenic potential of the strain or as a rapid screening and typing method. We recently developed a method to detect simultaneously 20 GAS virulence factors (spd3, sdc, sdaB, sdaD, speB, spyCEP, scpA, mac, sic, speL, K, M, C, I, A, H, G, J, smeZ and ssa) in four low volume multiplex PCR reactions (Borek et al., 2011) and below we present a detailed protocol describing the method.
Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warszawa, Poland.
Anna L
AL
Borek
Katarzyna
K
Obszańska
Waleria
W
Hryniewicz
Izabela
I
Sitkiewicz
D001426:Bacterial Proteins / Q000032:analysis*; D060885:Multiplex Polymerase Chain Reaction; D013290:Streptococcal Infections / Q000382:microbiology; D013297:Streptococcus pyogenes / Q000145:classification / Q000472:pathogenicity*; D037521:Virulence Factors / Q000032:analysis*
D001426:Bacterial Proteins; D037521:Virulence Factors