Serum vascular adhesion protein-1 correlates with vascular endothelial growth factor in patients with type II diabetes.
OBJECTIVE
To study serum levels of soluble vascular adhesion protein (sVAP)-1 in type II diabetic patients with retinopathy.


METHODS
Serum samples were obtained from 53 consecutive patients, including 14 cases with non-angiogenic ocular diseases, i.e., epiretinal membrane (ERM) and idiopathic macular hole (MH), 19 cases with age-related macular degeneration (AMD), and 20 cases with diabetic retinopathy (DR). Protein levels of sVAP-1, intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1, and vascular endothelial growth factor (VEGF) were determined by enzyme-linked immunosorbent assay. Enzymatic activity of semicarbazide-sensitive amine oxidase (SSAO) was also measured.


RESULTS
Serum level of sVAP-1 showed a moderate correlation with SSAO activity in all cases. Patients with DR had higher levels of serum sVAP-1 than subjects with ERM and MH, or those with AMD; however, severity of DR is not related to the serum levels of sVAP-1. Serum sVAP-1 correlated positively with VEGF in patients with DR, but not in those with ERM and MH, or those with AMD. Neither soluble ICAM-1 nor VCAM-1 correlated with VEGF, even in subjects with DR.


CONCLUSIONS
The current data demonstrate the elevated serum levels of sVAP-1 and correlation between sVAP-1 and VEGF in patients with type II diabetes.
Laboratory of Retinal Cell Biology, Keio University School of Medicine, Tokyo, Japan.
Nami
N
Yoshikawa
Kousuke
K
Noda
Hajime
H
Shinoda
Atsuro
A
Uchida
Yoko
Y
Ozawa
Kazuo
K
Tsubota
Yukihiko
Y
Mashima
Susumu
S
Ishida
D000368:Aged; D000369:Aged, 80 and over; D006631:Amine Oxidase (Copper-Containing) / Q000097:blood* / Q000737:chemistry; D015815:Cell Adhesion Molecules / Q000097:blood* / Q000737:chemistry; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D003930:Diabetic Retinopathy / Q000097:blood* / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D019773:Epiretinal Membrane / Q000097:blood / Q000150:complications; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood; D008268:Macular Degeneration / Q000097:blood / Q000150:complications; D008297:Male; D008875:Middle Aged; D012167:Retinal Perforations / Q000097:blood / Q000150:complications; D012720:Severity of Illness Index; D012995:Solubility; D015854:Up-Regulation; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
D015815:Cell Adhesion Molecules; C573420:ICAM1 protein, human; C467484:VEGFA protein, human; D019010:Vascular Cell Adhesion Molecule-1; D042461:Vascular Endothelial Growth Factor A; D018799:Intercellular Adhesion Molecule-1; C076668:AOC3 protein, human; D006631:Amine Oxidase (Copper-Containing)