Enhanced mixotrophic growth of microalga Chlorella sp. on pretreated swine manure for simultaneous biofuel feedstock production and nutrient removal.
The objectives were to assess the feasibility of using fermented liquid swine manure (LSM) as nutrient supplement for cultivation of Chlorella sp. UMN271, a locally isolated facultative heterotrophic strain, and to evaluate the nutrient removal efficiencies by alga compared with those from the conventionally decomposed LSM-algae system. The results showed that addition of 0.1% (v/v) acetic, propionic and butyric acids, respectively, could promote algal growth, enhance nutrient removal efficiencies and improve total lipids productivities during a 7-day batch cultivation. Similar results were observed when the acidogenic fermentation was applied to the sterilized and raw digested LSM rich in volatile fatty acids (VFAs). High algal growth rate (0.90 d(-1)) and fatty acid content (10.93% of the dry weight) were observed for the raw VFA-enriched manure sample. Finally, the fatty acid profile analyses showed that Chlorella sp. grown on acidogenically digested manure could be used as a feedstock for high-quality biodiesel production.
Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Ave., St. Paul, MN 55108, United States.
Bing
B
Hu
Min
M
Min
Wenguang
W
Zhou
Zhenyi
Z
Du
Michael
M
Mohr
Paul
P
Chen
Jun
J
Zhu
Yanling
Y
Cheng
Yuhuan
Y
Liu
Roger
R
Ruan
D000143:Acids / Q000378:metabolism; D000641:Ammonia / Q000302:isolation & purification; D000693:Anaerobiosis / Q000187:drug effects; D000818:Animals; D056804:Biofuels / Q000032:analysis*; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development*; D005232:Fatty Acids, Volatile / Q000494:pharmacology; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008372:Manure / Q000032:analysis*; D008697:Methane / Q000032:analysis; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development*; D009584:Nitrogen / Q000302:isolation & purification*; D010758:Phosphorus / Q000302:isolation & purification*; D013552:Swine
D000143:Acids; D056804:Biofuels; D005232:Fatty Acids, Volatile; D008372:Manure; D010758:Phosphorus; D000641:Ammonia; D009584:Nitrogen; D008697:Methane