Recognition of the let-7g miRNA precursor by human Lin28B.
Mammalian homologs of lin28: Lin28 and Lin28B block the post-transcriptional processing of the let-7 family of miRNAs. We report that in vitro the terminal stem-loop region of the let-7g miRNA precursor (pre-let-7g) required to bind Lin28B is restricted to 24 nucleotides (nt) including the 3' GGAG motif. Additionally, full length Lin28B is required for efficient binding to pre-let-7g and the stoichiometry of the complex is 1:1. Molecular dynamics (MD) simulations reveal the interactions of the pre-let-7g stem-loop and the GGAG motif in the stem region to the cold shock domain (CSD) and to the zinc knuckle domain (ZKD) of Lin28B, respectively.
Institute of Biophysical Chemistry, Goethe University, Frankfurt 60438, Germany. shaikpakeer@gmail.com
P Shaik Syed
PS
Ali
Umesh
U
Ghoshdastider
Jan
J
Hoffmann
Bernd
B
Brutschy
Slawomir
S
Filipek
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006860:Hydrogen Bonding; D013058:Mass Spectrometry; D035683:MicroRNAs / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012322:RNA Precursors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins; D017386:Sequence Homology, Amino Acid
D004268:DNA-Binding Proteins; C516326:LIN28B protein, human; D035683:MicroRNAs; D012322:RNA Precursors; D016601:RNA-Binding Proteins; C487931:mirnlet7 microRNA, human