Possible correlation between selenoprotein W and myogenic regulatory factors in chicken embryonic myoblasts.
The biological function of selenium (Se) is mainly elicited through Se-containing proteins. Selenoprotein W (SelW), one member of the selenoprotein family, is essential for the normal function of the skeletal muscle system. To investigate the possible relationship of Se in the process of differentiation in chicken myoblasts and the expression of SelW, the cultured chicken embryonic myoblasts were incubated with sodium selenite at different concentrations for 72 h, and then the mRNA levels of SelW and myogenic regulatory factors (MRFs) in myoblasts were determined at 12, 24, 48, and 72 h, respectively. Furthermore, the correlation between SelW mRNA expression and MRF mRNA expression was assessed. The results showed that the sodium selenite medium enhanced the mRNA expression of SelW, Myf-5, MRF4, and myogenin in chicken myoblasts. The mRNA expression levels of MRFs were significantly correlated with those of SelW at 24, 48, and 72 h. These data demonstrate that Se is involved in the differentiation of chicken embryonic myoblasts, and SelW showed correlation with MRFs.
Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Qiong
Q
Wu
Hai-Dong
HD
Yao
Zi-Wei
ZW
Zhang
Bo
B
Zhang
Fan-Yu
FY
Meng
Shi-Wen
SW
Xu
Xiao-Long
XL
Wang
D000818:Animals; D017849:Animals, Inbred Strains; D030161:Avian Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002642:Chick Embryo; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D051787:Myogenic Regulatory Factor 5 / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018006:Myogenic Regulatory Factors / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018008:Myogenin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D009994:Osmolar Concentration; D012333:RNA, Messenger / Q000378:metabolism; D012643:Selenium / Q000378:metabolism*; D051151:Selenoprotein W / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018038:Sodium Selenite / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
D030161:Avian Proteins; D051787:Myogenic Regulatory Factor 5; D018006:Myogenic Regulatory Factors; D018008:Myogenin; D012333:RNA, Messenger; D051151:Selenoprotein W; C063403:myogenic factor 6; D012643:Selenium; D018038:Sodium Selenite