Glucose sensor based on an electrochemical reduced graphene oxide-poly(L-lysine) composite film modified GC electrode.
A convenient and environmentally friendly method of fabricating glucose biosensors is proposed. Glucose oxidase (GOD) was immobilized on electrochemically reduced graphene oxide (ERGO) which was adsorbed on the poly-L-lysine (PLL) modified glassy carbon electrode after being immersed in GO solution for 4 h. The electrochemical behaviors of GOD/ERGO/PLL/GC electrode have been investigated by cyclic voltammetry. Direct electron transfer between GOD immobilized with ERGO/PLL and GC electrode was observed. Moreover, the GOD/ERGO/PLL/GC electrode exhibited excellent electrocatalytic activity for the detection of glucose with a linear range from 0.25 to 5 mmol L(-1).
Life and Environment Science College, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, P.R. China.
Liang
L
Hua
Xiaqin
X
Wu
Rong
R
Wang
D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005898:Glass / Q000737:chemistry; D005947:Glucose / Q000032:analysis* / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry*
D004800:Enzymes, Immobilized; D010087:Oxides; D011107:Polylysine; D002244:Carbon; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose