Comparison of cell-mediated immunity induced by three commercial single-dose Mycoplasma hyopneumoniae bacterins in pigs.
This study was to compare the degree of cell-mediated immunity induced by three commercial Mycoplasma hyopneumoniae bacterins using interferon-γ (IFN-γ) measurements, lymphocyte stimulation assays and delayed-type hypersensitivity tests. Serum IFN-γ levels were significantly elevated in all four vaccinated pig groups at 21 days post-vaccination (P<0.05). Lymphocytes isolated 21 days post-vaccination exhibited significantly more proliferation in response to M. hyopneumoniae than lymphocytes isolated 0 day pre-vaccination (P<0.05). Following intradermal injection of M. hyopneumoniae antigen at 14, 21 or 28 days post-vaccination, all pigs in the four vaccinated groups displayed skin reactions characterized by circumscribed, often erythematous nodules. Taken together, these demonstrate that all three commercial single-dose M. hyopneumoniae bacterins used in this study induce varying degrees of cell-mediated immunity.
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Hwi Won
HW
Seo
Kiwon
K
Han
Yeonsu
Y
Oh
Changhoon
C
Park
Eun Jeong
EJ
Choo
Sung-Hoon
SH
Kim
Bog-Hieu
BH
Lee
Chanhee
C
Chae
D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D007111:Immunity, Cellular / Q000502:physiology*; D008214:Lymphocytes / Q000378:metabolism; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology / Q000517:prevention & control*; D013552:Swine
D001428:Bacterial Vaccines