Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae.
Trs130 is a specific component of the transport protein particle II complex, which functions as a guanine nucleotide exchange factor (GEF) for Rab GTPases Ypt31/32. Ypt31/32 is known to be involved in autophagy, although the precise mechanism has not been thoroughly studied. In this study, we investigated the potential involvement of Trs130 in autophagy and found that both the cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy were defective in a trs130ts (trs130 temperature-sensitive) mutant. Mutant cells could not transport Atg8 and Atg9 to the pre-autophagosomal structure/phagophore assembly site (PAS) properly, resulting in multiple Atg8 dots and Atg9 dots dispersed in the cytoplasm. Some dots were trapped in the trans-Golgi. Genetic studies showed that the effect of the Trs130 mutation was downstream of Atg5 and upstream of Atg1, Atg13, Atg9 and Atg14 on the autophagic pathway. Furthermore, overexpression of Ypt31 or Ypt32, but not of Ypt1, rescued autophagy defects in trs130ts and trs65ts (Trs130-HA Trs120-myc trs65Δ) mutants. Our data provide mechanistic insight into how Trs130 participates in autophagy and suggest that vesicular trafficking regulated by GTPases/GEFs is important in the transport of autophagy proteins from the trans-Golgi to the PAS.
College of Life Sciences, Key Laboratory of Agricultural Environmental Microbiology of Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China.
Shenshen
S
Zou
Yong
Y
Chen
Yutao
Y
Liu
Nava
N
Segev
Sidney
S
Yu
Yan
Y
Liu
Gaoyi
G
Min
Min
M
Ye
Yan
Y
Zeng
Xiaoping
X
Zhu
Bing
B
Hong
Lars Olof
LO
Björn
Yongheng
Y
Liang
Shaoshan
S
Li
Zhiping
Z
Xie
D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D001343:Autophagy / Q000235:genetics*; D003593:Cytoplasm / Q000378:metabolism; D009154:Mutation; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D021601:trans-Golgi Network / Q000378:metabolism
D033942:Adaptor Proteins, Vesicular Transport; D029701:Saccharomyces cerevisiae Proteins; C475918:TRS130 protein, S cerevisiae; C080595:TRS65 protein, S cerevisiae; D033921:Vesicular Transport Proteins; C087905:YPT31 protein, S cerevisiae; C060755:YPT1 protein, S cerevisiae; C121633:YPT32 protein, S cerevisiae; D020691:rab GTP-Binding Proteins