An α-carbonic anhydrase from the thermophilic bacterium Sulphurihydrogenibium azorense is the fastest enzyme known for the CO2 hydration reaction.
We report the biochemical characterization of a new carbonic anhydrase (CA, EC 4.2.1.1), named SazCA, identified by translated genome inspection in Sulfurihydrogenibium azorense, a thermophilic bacterium from terrestrial hot springs of the Azores. SazCA is an α-CA showing kinetic parameters that make it the fastest enzyme of the CA family described so far. The biochemical properties, thermostability and inhibition of SazCA were compared with those of the thermophilic and mesophilic counterparts, demonstrating the special features of this unique enzyme.
CNR, Istituto di Biochimica delle Proteine (IBP), Via P. Castellino 111, 80131 Napoli, Italy.
Viviana De
VD
Luca
Daniela
D
Vullo
Andrea
A
Scozzafava
Vincenzo
V
Carginale
Mosè
M
Rossi
Claudiu T
CT
Supuran
Clemente
C
Capasso
D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D002245:Carbon Dioxide / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000145:classification / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D013696:Temperature; D014867:Water / Q000378:metabolism*
D014867:Water; D002245:Carbon Dioxide; D002256:Carbonic Anhydrases