Sonodynamically induced antitumor effects of 5-aminolevulinic acid and fractionated ultrasound irradiation in an orthotopic rat glioma model.
The sonodynamically induced selective antitumor effects of 5-aminolevulinic acid (5-ALA) on a C6 glioma that was implanted in a rat brain were evaluated. One week after the inoculation of the brains with the C6 rat glioma cells, glioma development was monitored using a 1.5 T MRI. Brains both with and without intravenous administration of 5-ALA (60 mg/kg body weight) or Radachlorin (40 mg/kg body weight) were insonated by a 1 MHz ultrasound at a dose of 2.65 W/cm(2). Irradiation was performed in a fractionated manner to avoid any thermal effects in the tissue due to the focused ultrasound; 16 min of irradiation were followed by a 3 min recess, then 4 min of resumed irradiation. Mean tumor sizes, measured after the rats were sacrificed 2 weeks post treatment, were 122.48 ± 39.64 mm(3) in sham-operated rats, 87.42 ± 21.40 mm(3) in rats receiving ultrasound without 5-ALA, 10.50 ± 8.20 mm(3) in rats receiving ultrasound with 5-ALA, and 56.42 ± 12.48 mm(3) in rats receiving ultrasound with Radachlorin. The tumor size was significantly smaller in the therapy group receiving sonodynamic 5-ALA than in any of the other groups (p < 0.05). This experimental rat model showed that sonodynamic therapy can be useful in the treatment of deep-seated malignant gliomas.
Department of Biomedical Engineering, School of Medicine, Catholic University of Daegu, Daegu, Korea.
Eun-Ju
EJ
Jeong
Seung-Jun
SJ
Seo
Young-Joon
YJ
Ahn
Ki-Hwan
KH
Choi
Ki-Hong
KH
Kim
Jong-Ki
JK
Kim
D000622:Aminolevulinic Acid / Q000627:therapeutic use*; D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001932:Brain Neoplasms / Q000473:pathology / Q000628:therapy*; D005260:Female; D005910:Glioma / Q000473:pathology / Q000628:therapy*; D019047:Phantoms, Imaging; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014464:Ultrasonic Therapy
D000970:Antineoplastic Agents; D017319:Photosensitizing Agents; D000622:Aminolevulinic Acid