Proteomic analysis of proteins of Weissella confusa 31 affected by bile salts.
Weissella confusa 31, an isolate from human feces, possesses desirable properties as a probiotic strain, including bile salt resistance. W. confusa 31 is not inhibited by bile salts up to 0.3% concentration. Proteins affected by bile salts (0.05%) were examined by 2-D gel electrophoresis. Our proteomic analyses revealed that the intensities of 29 spots were changed, where 17 increased (including 2 spots observed only under the bile salts stress conditions) and 12 decreased. Proteins were identified by MALDI-TOF mass spectrometry. Proteins increased in the band intensities included adenylate kinase (12.75-fold increase), Clp-like ATP-dependent protease (11.91-fold), 6-phosphogluconate dehydrogenase (10.35- fold), and HSP 70 (5.07-fold). Some of the increased or decreased proteins are also known to be involved in other types of stress responses.
Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University, Jinju 660-701, Korea.
Kang Wook
KW
Lee
Seung-Gyu
SG
Lee
Nam Soo
NS
Han
Jeong-Hwan
JH
Kim
D000263:Adenylate Kinase / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D001647:Bile Acids and Salts / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D050296:Microbial Viability; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D019936:Probiotics / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013312:Stress, Physiological; D058836:Weissella / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
D001426:Bacterial Proteins; D001647:Bile Acids and Salts; D018840:HSP70 Heat-Shock Proteins; D020543:Proteome; D010734:Phosphogluconate Dehydrogenase; D000263:Adenylate Kinase