BmNPV resistance of silkworm larvae resulting from the ingestion of TiO₂ nanoparticles.
Bombyx mori nucleopolyhedrovirus (BmNPV) causes infection in the silkworm that is often lethal. The infection is hard to prevent, partly because of the nature of the virus particles and partly because of the different strains of B. mori. Titanium dioxide nanoparticles (TiO₂ NPs) have been demonstrated to have antimicrobial properties. The present study investigated whether TiO₂ NPs added to an artificial diet can increase the resistance of B. mori larvae to BmNPV and examined the molecular mechanism behind any resistance shown. The results indicated that ingested TiO₂ NPs decreased reactive oxygen species and NO accumulation in B. mori larvae under BmNPV infection, which in turn led to a decrease in their growth inhibition and mortality. In addition, the TiO₂ NPs significantly promoted the expression of resistance-related genes, including those encoding superoxide dismutase, catalase, glutathione peroxidase, acetylcholine esterase, carboxylesterase, heat shock protein 21, glutathione S transferase o1, P53, and transferring and of genes encoding cytochrome p302 and nitric oxide synthase. These findings are a useful addition to the understanding of the mechanism of BmNPV resistance of B. mori larvae in response to TiO₂ NPs addition. Such information also provides a theoretical basis for the use of TiO₂ NPs in sericulture.
Medical College, Soochow University, Suzhou, 215123, People's Republic of China.
Bing
B
Li
Yi
Y
Xie
Zhe
Z
Cheng
Jie
J
Cheng
Rengping
R
Hu
Suxin
S
Gui
Xuezi
X
Sang
Qingqing
Q
Sun
Xiaoyang
X
Zhao
Lei
L
Sheng
Weide
W
Shen
Fashui
F
Hong
D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage* / Q000494:pharmacology; D012831:Bombyx / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism / Q000821:virology; D003433:Crosses, Genetic; D060467:Disease Resistance / Q000187:drug effects; D016503:Drug Delivery Systems; D024882:Drug Resistance, Viral / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D019476:Insect Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000821:virology; D015227:Lipid Peroxidation / Q000187:drug effects; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D009569:Nitric Oxide / Q000378:metabolism; D017924:Nucleopolyhedroviruses / Q000187:drug effects* / Q000276:immunology / Q000472:pathogenicity; D017382:Reactive Oxygen Species / Q000378:metabolism; D014025:Titanium / Q000008:administration & dosage* / Q000494:pharmacology
D000998:Antiviral Agents; D019476:Insect Proteins; D017382:Reactive Oxygen Species; C009495:titanium dioxide; D009569:Nitric Oxide; D014025:Titanium