Endemic angiostrongyliasis in the Brazilian Amazon: natural parasitism of Angiostrongylus cantonensis in Rattus rattus and R. norvegicus, and sympatric giant African land snails, Achatina fulica.
Angiostrongylus cantonensis, the rat lungworm, is one etiological agent of eosinophilic meningoencephalitis in humans. This zoonosis is frequently found in Asia and, more recently, in North America, Caribbean Island and northeastern of South America. Until now, research of A. cantonensis in southern, southeastern and northeastern regions of Brazil has been found natural infections only terrestrial and freshwater intermediate snail hosts (Achatina fulica, Sarasinula marginata, Subulina octona, Bradybaena similaris and Pomacea lineate). In this study, we examined the occurrence of helminthes in the synantropic rodents Rattus rattus and Rattus norvegicus in northern Brazil, focusing on the role of these species as vertebrate hosts of A. cantonensis and A. fulica as intermediate host have found natural. Thirty specimens of R. rattus and twelve of R. norvegicus were collected in the Guamá and Jurunas neighborhoods of the city of Belém, in the Brazilian state of Pará, of which almost 10% harbored adult worms in their pulmonary arteries. Sympatric A. fulica were found to be infected by L(3) larvae, which experimental infection confirmed to be A. cantonensis. Natural infection of snails and rodents with A. cantonensis was confirmed through morphological and morphometrical analyses of adults and larvae using light microscopy, scanning electron microscopy and molecular sequences of partial Cytochrome Oxidase subunit I. Phylogenetic analyses showed that A. cantonensis isolated from Pará, Brazil is similar to Japan isolate; once these specimens produced a single haplotype with high bootstrap support with Rio de Janeiro isolate. This study confirms that A. cantonensis is now endemic in northern Brazil, and that R. rattus and R. norvegicus act as natural definitive hosts, and A. fulica as the intermediate host of the parasite in this region.
Laboratório de Biologia Celular e Helmintologia, Profa. Dra. Reinalda Marisa Lanfredi, Universidade Federal do Pará, Instituto de Ciências Biológicas, St. Augusto Corrêa, 01 - Guamá, Belém, 66075-110 Pará, Brazil.
V L C
VL
Moreira
E G
EG
Giese
F T V
FT
Melo
R O
RO
Simões
S C
SC
Thiengo
A
A
Maldonado
J N
JN
Santos
D017185:Angiostrongylus cantonensis / Q000302:isolation & purification*; D000818:Animals; D001938:Brazil / Q000453:epidemiology; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D019353:Endemic Diseases; D005260:Female; D006239:Haplotypes; D008297:Male; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D011651:Pulmonary Artery / Q000469:parasitology; D051381:Rats; D012376:Rodent Diseases / Q000453:epidemiology* / Q000469:parasitology* / Q000473:pathology; D017422:Sequence Analysis, DNA; D012908:Snails / Q000469:parasitology*; D017206:Strongylida Infections / Q000453:epidemiology / Q000469:parasitology / Q000473:pathology / Q000662:veterinary*
D018509:DNA, Helminth; D003576:Electron Transport Complex IV