Incorporation of disulfide containing protein modules into multivalent antigenic conjugates: generation of antibodies against the thrombin-sensitive region of murine protein S.
Antigenic peptide conjugates can be used as vaccines and for the production of antibodies for clinical and research use. A method is presented here for the construction of conjugates incorporating oxidatively folded protein domains in their native conformation. This method was used to prepare multiple antigenic peptide constructs of the thrombin-sensitive loop region of murine anticoagulant protein S.
Department of Biochemistry, Maastricht University, Maastricht, The Netherlands. p.vandevijver@maastrichtuniversity.nl
Pieter
P
Van de Vijver
Martin
M
Schmitt
Dennis
D
Suylen
Liesbeth
L
Scheer
M Christella L G D
MC
Thomassen
Leon J
LJ
Schurgers
John H
JH
Griffin
Rory R
RR
Koenen
Tilman M
TM
Hackeng
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D004220:Disulfides / Q000737:chemistry*; D051379:Mice; D008958:Models, Molecular; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D011487:Protein Conformation; D017293:Protein S / Q000737:chemistry* / Q000276:immunology*; D013917:Thrombin / Q000378:metabolism*
D000906:Antibodies; D000941:Antigens; D004220:Disulfides; D010455:Peptides; D017293:Protein S; D013917:Thrombin