Simultaneous determination of steviol and steviol glycosides by liquid chromatography-mass spectrometry.
A direct, versatile method for the determination of steviol and nine steviol glycosides in food products has been developed by using electrospray ionisation liquid chromatography-mass spectrometry in the negative-ion mode. Ten stevia compounds were readily separated on an amino column by using a gradient separation. Data for analyte quantification were collected in the selected ion monitoring mode, giving the method limit of detection of 0.01-0.34 µg g⁻¹ and repeatability at the limit of quantitation of 2%-15% relative standard deviation. Thirty-four commercially available food products were tested by using the optimised method, and in these products rebaudioside A and stevioside comprised 52%-100% of the total steviol glycosides. Multiple reaction monitoring data were collected to provide analyte confirmation. Stability data for rebaudioside A stored at room temperature, 40°C and 60°C over a period of 1-14 days are shown.
Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740, USA. romina.shah@fda.hhs.gov
Romina
R
Shah
Lowri S
LS
De Jager
Timothy H
TH
Begley
D001628:Beverages / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D045786:Diterpenes, Kaurane / Q000032:analysis* / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling; D061353:Food Storage; D057141:Food, Preserved / Q000032:analysis; D005960:Glucosides / Q000032:analysis / Q000737:chemistry; D006027:Glycosides / Q000032:analysis*; D006358:Hot Temperature; D057230:Limit of Detection; D015394:Molecular Structure; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D013549:Sweetening Agents / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D013997:Time Factors
D045786:Diterpenes, Kaurane; D005960:Glucosides; D006027:Glycosides; D013549:Sweetening Agents; C012043:stevioside; C012042:steviol; C025474:rebaudioside A