Molecular detection and occurrence of 'Candidatus Mycoplasma haemobos' in dairy cattle of Southern Brazil.
Bovine hemoplasmas are bacteria found on the erythrocyte surface or free in the plasma of cattle. The aim of the present study was to evaluate the occurrence of 'Candidatus Mycoplasma haemobos' ('C. M. haemobos') in Holstein and Jersey cattle raised in Londrina and surroundings, northern region of the State of Parana, Southern Brazil. PCR testing directed to 16S rRNA gene fragment was performed to investigate the occurrence and characterize the molecular identity of 'C. M. haemobos'. A total of 264/433 (60.97%) blood samples were positive by PCR. Further alignment of 500-bp amplicons to available sequences at the GenBank database showed high identity (100%) to 'C. M. haemobos'. To the author's knowledge, this is the first molecular confirmation of the hemoplasma 'C. M. haemobos' in cattle from Brazil. Moreover, 'C. M. haemobos' was observed in high occurrence in dairy cattle, and may have significant impact in livestock production.
Programa de Pós-graduação em Ciência Animal, Departamento de Medicina Veterinaria Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brasil.
Aline
A
Girotto
Amanda Fonseca
AF
Zangirólamo
Alexey Leon Gomel
AL
Bogado
Arnaldo Sotero Luz E
AS
Souza
Gislaine Cristina Ferreira
GC
da Silva
João Luis
JL
Garcia
Laurival Antônio
LA
Vilas Boas
Alexander Welker
AW
Biondo
Odilon
O
Vidotto
D000818:Animals; D001938:Brazil; D002417:Cattle / Q000097:blood*; D003612:Dairying; D005260:Female; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction