Segmental labeling to study multidomain proteins.
This chapter contains a review of methodologies and recent applications of segmental labeling for NMR structural studies of proteins and protein complexes. Segmental labeling is used to specifically label a segment of protein structure with NMR active nuclei, thus reducing NMR spectral complexity and greatly facilitating structural NMR studies of large multi-domain proteins. It can also be used to introduce a synthetic fragment into a protein structure to study post-translationally modified proteins. Detailed protocols describing segmental labeling techniques are also included.
Department of Chemistry, State University of New York, Albany, NY 12222, USA.
Jing
J
Xue
David S
DS
Burz
Alexander
A
Shekhtman
D006031:Glycosylation; D007553:Isotope Labeling / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D060327:Solid-Phase Synthesis Techniques
D011506:Proteins