KPC-4 Is encoded within a truncated Tn4401 in an IncL/M plasmid, pNE1280, isolated from Enterobacter cloacae and Serratia marcescens.
We describe the transfer of bla(KPC-4) from Enterobacter cloacae to Serratia marcescens in a single patient. DNA sequencing revealed that KPC-4 was encoded on an IncL/M plasmid, pNE1280, closely related to pCTX-M360. Further analysis found that KPC-4 was encoded within a novel Tn4401 element (Tn4401f) containing a truncated tnpA and lacking tnpR, ISKpn7 left, and Tn4401 IRL-1, which are conserved in other Tn4401 transposons. This study highlights the continued evolution of Tn4401 transposons and movement to multiple plasmid backbones that results in acquisition by multiple species of Gram-negative bacilli.
Departments of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Kendall A
KA
Bryant
Trevor C
TC
Van Schooneveld
Ishwor
I
Thapa
Dhundy
D
Bastola
Laurina O
LO
Williams
Thomas J
TJ
Safranek
Steven H
SH
Hinrichs
Mark E
ME
Rupp
Paul D
PD
Fey
D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D060085:Coinfection; D004251:DNA Transposable Elements; D016972:Enterobacter cloacae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000188:drug therapy / Q000382:microbiology; D017809:Fatal Outcome; D005260:Female; D022761:Gene Transfer, Horizontal; D006801:Humans; D008875:Middle Aged; D010957:Plasmids; D017422:Sequence Analysis, DNA; D016868:Serratia Infections / Q000188:drug therapy / Q000382:microbiology; D012706:Serratia marcescens / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D001618:beta-Lactamases