Live birth of twins after IVF of oocytes that were cryopreserved almost 12 years before.
A 45-year-old woman received embryos from IVF by intracytoplasmic sperm injection (ICSI) with her own oocytes that were cryopreserved (slow freezing in a low-sodium medium) 11 years and 7 and a half months before, when she was 33 years old. From seven metaphase-II oocytes thawed, five survived, four were fertilized after ICSI and two cleaving embryos were transferred on day 3. A diamniotic dichorionic term pregnancy was achieved, ending with the delivery of two healthy girls. As far as is known, this case represents, to date, the longest storage period of cryopreserved human oocytes resulting in a live birth.
Halitus Instituto Médico, Marcelo T. de Alvear 2084, C1122AAF Buenos Aires, Argentina. carlos.quintans@halitus.com
Carlos J
CJ
Quintans
Monica J
MJ
Donaldson
M Fernanda
MF
Urquiza
Inés
I
Carretero
R Agustín
RA
Pasqualini
Marcos
M
Horton
R Sergio
RS
Pasqualini
D015925:Cryopreservation; D004477:Ectogenesis; D004624:Embryo Transfer; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D050498:Live Birth; D008875:Middle Aged; D009865:Oocytes; D011247:Pregnancy; D059285:Pregnancy, Twin; D020554:Sperm Injections, Intracytoplasmic; D013997:Time Factors