Sulfated modification can enhance antiviral activities of Achyranthes bidentata polysaccharide against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro.
Achyranthes bidentata polysaccharide (ABPS) was sulfated using the chlorosulfonic acid-pyridine method. The UV-spectrum of sulfated ABPS (sABPS) was characterized with two different absorbance peaks at 196.3 and 262.8 nm. FT-IR spectrum of sABPS exhibited absorption bands at 1236.5 cm(-1) (asymmetrical S=O stretching vibration) and 822.8 cm(-1) (symmetrical C-O-S vibration related to a C-O-SO(3) group). Anti-PRRSV activities of sABPS were tested on MARC-145 cells by MTT method. The results showed that sABPS exhibited significant antiviral activities at lower concentrations in comparison with the non-modified ABPS treated cells, indicating that sulfated modification could enhance antiviral activities of ABPS. These findings may provide the potential value to develop a new-type drug against PRRSV infection.
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, PR China.
Chuanmin
C
Liu
Huijuan
H
Chen
Kun
K
Chen
Yanfeng
Y
Gao
Song
S
Gao
Xiufan
X
Liu
Jingui
J
Li
D029044:Achyranthes / Q000737:chemistry*; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002522:Chlorocebus aethiops; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000188:drug therapy*; D019316:Porcine respiratory and reproductive syndrome virus; D013552:Swine
D000998:Antiviral Agents; D011134:Polysaccharides