[Cell sheet technology and its application in bone tissue engineering].
OBJECTIVE
To review the progress of cell sheet technology (CST) and its application in bone tissue engineering.


METHODS
The literature concerning CST and its application was extensively reviewed and analyzed.


RESULTS
CST using temperature-responsive culture dishes is applied to avoid the shortcomings of traditional tissue engineering. All cultured cells are harvested as intact sheets along with their deposited extracellular matrix. Avoiding the use of proteolytic enzymes, cell sheet composed of the cells and extracellular matrix derived from the cells, and remained the relative protein and biological activity factors. Consequently, cell sheet can provide a suitable microenvironment for the bone regeneration in vivo. With CST, cell sheet engineering is allowed for tissue regeneration by the creation of three-dimensional structures via the layering of individual cell sheets, be created by wrapping scaffold with cell sheets, or be created by folding the cell sheets, showing great potential in tissue engineered bone.


CONCLUSIONS
Constructing tissue engineered bone using CST and traditional method of bone tissue engineering will promote the development of the bone tissue engineering.
Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Guangxi Medical University, Nanning Guangxi 530021, PR China.
Yali
Y
Chen
Nuo
N
Zhou
Xuanping
X
Huang
D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis / Q000502:physiology*; D013696:Temperature; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D016378:Tissue Transplantation / Q000379:methods
D001672:Biocompatible Materials