Subcutaneous tissue responses to three endodontic irrigants: a comparative study.
BACKGROUND
This study aimed to compare the subcutaneous tissue responses to MTAD (mixture of a tetracycline isomer, an acid, and a detergent), 17% EDTA, and 2.6% NaOCl.


METHODS
Thirty-six Wistar albino rats were used for this study. Test solutions were injected subcutaneously into predetermined areas on the animal dorsum. The rats were then randomly divided into three groups of twelve each and sacrificed at 2 hours, 2 days, and 2 weeks. The severity of inflammation induced by each irrigant at different time intervals was assessed histologically. The data were analyzed using Kruskal-Wallis and Friedman tests.


RESULTS
The difference in severity of inflammatory reactions induced by tested irrigants at the different time intervals was statically significant (P<0.05). There was no significant difference between the severity of inflammation induced by MTAD and 2.6% NaOCl at the various time intervals (P>0.05). Subcutaneous tissue responses to MTAD were not different from those observed in 17% EDTA specimens at 2-hour and 2-day intervals (P>0.05).


CONCLUSIONS
Under the conditions of this study, MTAD has the same toxicity as 2.6% NaOCl.
Dental Research Center, Department of Endodontics, Dental School, Tehran University of Medical Sciences, Tehran, Iran.
Kazem
K
Ashofteh Yazdi
Mohammad
M
Sabeti
Pooriya
P
Motahhary
Alireza
A
Kolahdouzan
Mohsen
M
Shayesteh
Noushin
N
Shokouhinejad