Molecular characterization  of sour orange (Citrus aurantium) accessions and their relatives using  SSR and SRAP markers.
Citrus  production with its many varieties is of importance since it provides  economically important products for Turkish exports. Sour orange is a rootstock  commonly used for propagating the different scion varieties. Knowledge of the  genetic diversity of the rootstock accessions would be useful in order to  improve citrus breeding programs. We studied genetic relationships and  diversity of 51 accessions of sour orange (Citrus aurantium) and their  relatives using SSR (simple sequence repeat) and SRAP (sequence-related  amplified polymorphism) molecular markers. Twenty-one SRAP primer combinations  were tested on these accessions and relatives, producing 167 polymorphic  fragments, with a mean of 8.0 and a mean polymorphism information content value  of 0.47. Seventeen SSR primers also produced 30 polymorphic fragments, with a  mean of 1.4 per primer and a mean polymorphism information content value of  0.39. The unweighted pair-group method with arithmetic average analysis using  combined SSR and SRAP data showed a similarity range from 0.71 to 1.00 among  the accessions. In the cluster analysis, sour orange relatives were indicated  as a separate group from sour orange. 'Macrophylla' and 'Mexican lime' were the  accessions most distinct (0.71) from the others. We conclude that genetic  diversity in these sour orange accessions is lower and some of them were  identical.
Bati Akdeniz Agricultural Research Institute, Antalya, Turkey.
I
I
Polat
Y A
YA
Kacar
T
T
Yesiloglu
A
A
Uzun
O
O
Tuzcu
O
O
Gulsen
M
M
Incesu
G
G
Kafa
E
E
Turgutoglu
S
S
Anil
D001483:Base Sequence; D002957:Citrus / Q000235:genetics*; D060146:Ecotype; D005819:Genetic Markers; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic
D005819:Genetic Markers