Antiproliferative activity of flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the MCF7, KB, and NIH/3T3 cell lines.
This study assessed the antiproliferative effect in vitro of the flower hexane extract obtained from Mentha spicata associated with Mentha rotundifolia against the human breast adenocarcinoma (MCF-7), human mouth epidermal carcinoma (KB), and mouse embryonic fibroblast (NIH 3T3) cell lines, using sulforhodamine B (SRB) assay. A cell density of 2×10(4)/well was seeded in 96-well plates, and samples at different concentrations ranging from 10 to 500 mg/mL were tested. The optical density was determined in an ELISA multiplate reader (Thermo Plate TP-Reader). Results demonstrated that the hexane extract presented antiproliferative activity against both the tumor cell lines KB and MCF-7, presenting a GI(50) (MCF-7=13.09 mg/mL), TGI (KB=37.76 mg/mL), and IL(50) (KB=291.07 mg/mL). Also, the hexane extract presented antiproliferative activity toward NIH 3T3 cells GI(50) (183.65 mg/mL), TGI (280.54 mg/mL), and IL(50) (384.59 mg/mL). The results indicate that the flower hexane extract obtained from M. spicata associated with M. rotundifolia presents an antineoplastic activity against KB and MCF-7, although an antiproliferative effect at a high concentration of the extract was observed toward NIH 3T3.
Department of Physiology and Pharmacology, Institute of Biology, Federal University of Pelotas, Pelotas, Rio Grande del Sul, Brazil.
Fernanda
F
Nedel
Karine
K
Begnini
Pedro Henrique de Azambuja
PH
Carvalho
Rafael Guerra
RG
Lund
Fátima T A
FT
Beira
Francisco Augusto B
FA
Del Pino
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D035264:Flowers / Q000737:chemistry*; D006586:Hexanes; D006801:Humans; D007624:KB Cells; D061986:MCF-7 Cells; D027541:Mentha / Q000737:chemistry*; D031337:Mentha spicata / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D010936:Plant Extracts / Q000494:pharmacology*; D012235:Rhodamines / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D006586:Hexanes; D010936:Plant Extracts; D012235:Rhodamines; C022027:lissamine rhodamine B; C026385:n-hexane