Adoptive transfer of macrophages from adult mice reduces mortality in mice infected with human enterovirus 71.
Human enterovirus 71 (EV71) causes hand, foot and mouth disease in children under 6 years of age, and the neurological complications of this virus can lead to death. Until now, no vaccines or drugs have been available for the clinical control of this epidemic. Macrophages can engulf pathogens and mediate a series of host immune responses that play a role in the defence against infectious diseases. Using immunohistochemistry, we observed the localizations of virus in muscle tissues of EV71-infected mice. The macrophages isolated from the adult mice could kill the virus gradually in vitro, as shown using quantitative real-time PCR (qRT-PCR) and virus titration. Co-localisation of lysosomes and virus within macrophages suggested that the lysosomes were possibly responsible for the phagocytosis of EV71. Activation of the macrophages in the peritoneal cavity of mice four days pre-infection reduced the mortality of mice upon lethal EV71 infection. The adoptive transfer of macrophages from adult mice inhibited virus replication in the muscle tissues of infected mice, and this was followed by a relief of symptoms and a significant reduction of mortality, which suggested that the adoptive transfer of macrophages from adult humans represents a potential strategy to treat EV71-infected patients.
Key Laboratory of Human Diseases Comparative Medicine, Ministry of Health, Beijing, China.
Jiangning
J
Liu
Xiaoying
X
Li
Xiaoxu
X
Fan
Chunmei
C
Ma
Chuan
C
Qin
Lianfeng
L
Zhang
D019264:Adoptive Transfer; D000818:Animals; D004195:Disease Models, Animal; D029821:Enterovirus A, Human / Q000276:immunology* / Q000472:pathogenicity*; D004769:Enterovirus Infections / Q000276:immunology* / Q000401:mortality / Q000821:virology; D008247:Lysosomes / Q000821:virology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009132:Muscles / Q000821:virology; D010587:Phagocytosis; D016019:Survival Analysis