Amplified electrochemiluminescence detection of DNA-binding protein based on the synergy effect of electron and energy transfer between CdS nanocrystals and gold nanoparticles.
In this work, we firstly enunciated the presence of synergy effect between the electron and energy transfer in an electrochemiluminescence (ECL) system involving the CdS NCs and Au NPs, based on which an amplified ECL biosensor was constructed for sensitive detection of DNA-protein interactions. Specifically, Au NPs modified on electrode, following by assembled probe DNA. Then the modified GCE was hybridized with SiO(2)@CdS/DNA conjugates. With DNA duplex as a rigid spacer, Au NPs could not only accelerate the electron transfer but also produce the surface plasma resonance upon excited ECL of CdS NCs. However, the enhanced ECL could be greatly suppressed by the binding of target protein. The prepared biosensor possesses excellent analytical performance with the linear range of target protein from 0.015 to 150 nM.
State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
Jing
J
Wang
Wei-Wei
WW
Zhao
Hong
H
Zhou
Jing-Juan
JJ
Xu
Hong-Yuan
HY
Chen
D015374:Biosensing Techniques / Q000295:instrumentation; D019187:Cadmium Compounds / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation; D003460:Crystallization; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D004579:Electron Transport; D004735:Energy Transfer; D004867:Equipment Design; D019544:Equipment Failure Analysis; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation*; D006046:Gold / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D045663:Quantum Dots; D015203:Reproducibility of Results; D018036:Selenium Compounds / Q000737:chemistry*; D012680:Sensitivity and Specificity
D019187:Cadmium Compounds; D004268:DNA-Binding Proteins; D018036:Selenium Compounds; D006046:Gold; D004247:DNA; C058667:cadmium selenide