NPRL2  gene expression in the progression of colon tumors.
Genetic and epigenetic factors affecting  DNA methylation and gene expression are known to be involved in the development  of colon cancer, but the full range of genetic alterations and many key genes  involved in the pathogenesis of colon cancer remain to be identified. NPRL2 is  a candidate tumor suppressor gene identified in the human chromosome 3p21.3  region. We evaluated the role of this gene in the pathogenesis of colorectal  cancer by investigating NPRL2 mRNA expression in 55 matched normal and tumor  colon tissue samples using quantitative RT-PCR analysis. There was  significantly decreased NPRL2 expression in 45% of the patients. Lower NPRL2  expression was observed significantly more frequently in poorly differentiated  tumor samples than in highly or moderately differentiated tumors. We conclude  that expression of NPRL2 contributes to progression of colon cancer.
Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University, Kocamustafapasa, Istanbul, Turkey.
B
B
Yogurtcu
I
I
Hatemi
I
I
Aydin
N
N
Buyru
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D014158:Transcription, Genetic; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
C492816:NPRL2 protein, human; D025521:Tumor Suppressor Proteins