Spanish black radish (Raphanus sativus L. Var. niger) diet enhances clearance of DMBA and diminishes toxic effects on bone marrow progenitor cells.
Vegetables of the Cruciferae family contain high levels of glucosinolates, metabolites of which are believed to enhance detoxification. Spanish black radishes (SBR) contain 4× more glucosinolates than other crucifers. This study examined whether feeding mice a diet containing 20% SBR for 2 wk could enhance metabolism of 7,12-dimethylbenz(a)anthracene (DMBA) and inhibit DMBA-mediated bone marrow toxicity. Expression of Phase I and II detoxification enzymes was significantly greater for mice fed SBR than control diet. Six hours after DMBA administration, the blood levels of DMBA in mice fed the SBR diet were significantly lower than mice fed a control diet. DMBA reduced bone marrow cells in mice fed control diet to a significantly greater extent than mice fed the SBR diet. Colony forming assays demonstrated that mice on the SBR diet had 1) less reduction in lymphoid CFU-preB progenitor cells, 2) greater recovery of CFU-preB progenitor cells at 168 h, and 3) less reduction of CFU-GM progenitor cells at 6 h. Therefore, mice fed a 20% SBR diet for 2 wk had greater expression of detoxification enzymes, faster metabolism of DMBA, and a reduction in DMBA-induced bone marrow toxicity. Overall, these results support the hypothesis that glucosinolates in SBR are protective against acute toxicity.
Department of Pathobiological Sciences and Molecular and Environmental Toxicology Center, University of Wisconsin, Madison, Wisconsin, USA.
Alhaji U
AU
N'jai
Michael Q
MQ
Kemp
Brandon T
BT
Metzger
Paul R
PR
Hanlon
Melissa
M
Robbins
Charles
C
Czuyprynski
David M
DM
Barnes
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000493:pharmacokinetics* / Q000633:toxicity; D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000378:metabolism; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005260:Female; D005961:Glucosinolates / Q000494:pharmacology; D055014:Granulocyte-Macrophage Progenitor Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010936:Plant Extracts / Q000494:pharmacology; D031224:Raphanus; D060888:Real-Time Polymerase Chain Reaction; D013030:Spain
D005961:Glucosinolates; D010936:Plant Extracts; D015127:9,10-Dimethyl-1,2-benzanthracene