Essential roles of PIKfyve and PTEN on phagosomal phosphatidylinositol 3-phosphate dynamics.
PtdIns(3)P (phosphatidylinositol 3-phosphate) is a signaling molecule important for phagosome maturation. The major role of Vps34 in production of phagosomal PtdIns(3)P has been indicated. However, the fate of the newly generated PtdIns(3)P has not been well described. Here we show that elimination of PtdIns(3)P from phagosomal membrane was significantly delayed in RAW264.7 macrophages lacking PTEN or PIKfyve. In the PTEN-deficient cells treated with a PIKfyve inhibitor, degradation of PtdIns(3)P was almost lost, indicating that PTEN and PIKfyve are two major players in phagosomal PtdIns(3)P metabolism.
Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan.
Kaoru
K
Hazeki
Kiyomi
K
Nigorikawa
Yuki
Y
Takaba
Tomohiro
T
Segawa
Akiko
A
Nukuda
Ayaka
A
Masuda
Yuki
Y
Ishikawa
Koji
K
Kubota
Shunsuke
S
Takasuga
Osamu
O
Hazeki
D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D005260:Female; D006575:Heterocyclic Compounds, 3-Ring / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D010588:Phagosomes / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D034622:RNA Interference
D000631:Aminopyridines; D006575:Heterocyclic Compounds, 3-Ring; D051059:PTEN Phosphohydrolase; D019869:Phosphatidylinositol 3-Kinases; D018129:Phosphatidylinositol Phosphates; D000081082:Phosphoinositide-3 Kinase Inhibitors; C540576:6-amino-N-(3-(4-(4-morpholinyl)pyrido(3',2'-4,5)furo(3,2-d)pyrimidin-2-yl)phenyl)-3-pyridinecarboxamide; C055525:phosphatidylinositol 3-phosphate; C116930:Pikfyve protein, mouse; C487757:Pten protein, mouse