Comparative study of the reactivity of natural and mutated streptokinase with total antistreptokinase antibodies in human sera.
Streptokinase is widely used as an anticoagulant drug for the treatment of heart attacks. Because of antibody production against injected drug in individuals consuming streptokinase and causing allergic reactions, streptokinase treatment effects become neutral. Recombinant mutant type of streptokinase was prepared by removing of 42 amino acids from the C terminal region. ELISA plates were coated by natural and mutant streptokinase as antigen. Ninety-six normal serum samples as well as 27 streptokinase consumer serum samples (patients with acute myocardial infraction) were analyzed. The results showed that serum antibodies against natural streptokinase were three times more than those against the mutated streptokinase. In case of preserving thrombolytic activity, mutated streptokinase can be used as an alternative of the natural form.
Cellular and Molecular Biology Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mojgan
M
Bandehpour
Zarrin
Z
Sharifnia
Nasrin
N
Mohajeri
Maryam
M
Taherkhani
Ameneh
A
Koochaki
Fatemeh
F
Yarian
Negar
N
Seyyed
Ramin
R
Shirvani
Parviz
P
Pakzad
Habibollah
H
Saadat
Bahram
B
Kazemi
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood* / Q000276:immunology; D000925:Anticoagulants / Q000276:immunology / Q000378:metabolism* / Q000627:therapeutic use*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008969:Molecular Sequence Data; D009203:Myocardial Infarction / Q000097:blood / Q000188:drug therapy* / Q000276:immunology; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000627:therapeutic use; D017384:Sequence Deletion; D013300:Streptokinase / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000627:therapeutic use*; D015912:Thrombolytic Therapy
D000907:Antibodies, Bacterial; D000925:Anticoagulants; D011994:Recombinant Proteins; C014338:antistreptokinase; D013300:Streptokinase