Regulation of synaptojanin 2 5'-phosphatase activity by Src.
Synaptojanin 2 (SYNJ2) is a phosphatidylinositol (PI) phosphatase that controls two distinct functions, clathrin-mediated endocytosis and tumor cell invadopodia formation and invasion. Here, we identify a number of novel SYNJ2 binding partners, several of which have previously been shown to be necessary for invadopodia formation or clathrin-mediated endocytosis. We focus on Src family kinases. We found that Src phosphorylates SYNJ2 on Tyr ( 490) , thereby stimulating SYNJ2 5'-phosphatase activity in vitro. We also provide evidence that Src-mediated phosphorylation of SYNJ2 contributes to invadopodia formation.
Center for Oncology and Cell Biology, The Feinstein Institute for Medical Research at North Shore-LIJ, Manhasset, NY, USA.
Yayu
Y
Chuang
Xiaonan
X
Xu
Aneta
A
Kwiatkowska
George
G
Tsapraillis
Hyonson
H
Hwang
Konstantinos
K
Petritis
Dan
D
Flynn
Marc
M
Symons
D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D022163:Clathrin-Coated Vesicles / Q000378:metabolism*; D051356:Cortactin / Q000235:genetics / Q000378:metabolism*; D004705:Endocytosis; D004789:Enzyme Activation; D057809:HEK293 Cells; D006801:Humans; D047468:Immunoprecipitation; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D051076:Proto-Oncogene Proteins c-fyn / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014443:Tyrosine / Q000378:metabolism; D018909:src Homology Domains; D019061:src-Family Kinases / Q000235:genetics / Q000378:metabolism; D000099182:Phosphoinositide 5-Phosphatases
D051356:Cortactin; D010744:Phosphoric Monoester Hydrolases; D051076:Proto-Oncogene Proteins c-fyn; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D014443:Tyrosine; D019061:src-Family Kinases; D000099182:Phosphoinositide 5-Phosphatases; C495868:CTTN protein, human; C494957:FYN protein, human; C060902:lyn protein-tyrosine kinase