Graphene oxide absorbed anti-IL10R antibodies enhance LPS induced immune responses in vitro and in vivo.
Interleukin 10 is an anti-inflammatory cytokine which limits immune responses to both self and foreign antigens. Blocking IL10 at the time of immunization increases cytotoxic T cell responses in antigen experienced host, a situation similar to therapeutic vaccination for cancer and chronic viral infection, where patients usually develop ineffective immune responses to tumour or viral antigens before immunotherapy starts. Graphene oxide (GO) is a nano material often used for drug delivery and tissue engineering. In the current paper, we demonstrated that GO is able to absorb anti-IL10 receptor antibodies. The anti-IL10R antibodies absorbed in GO are slowly released and the release of absorbed antibodies is pH dependent. GO absorbed anti-IL10R antibodies are bioactive both in vitro and in vivo. GO absorbed anti-IL10R antibodies are more efficient than free anti-IL10R antibodies at eliciting LPS stimulated CD8 T cell responses. Our results suggest that GO is able to absorb anti-IL10R antibodies and absorbed anti-IL10R antibody may be useful as an adjuvant for vaccination and ideal for delivering to tumour site, and breaking of suppressive tumour environment.
Illawarra Health and Medical Research Institute, Graduate School of Medicine, University of Wollongong, Australia.
Guoying
G
Ni
Yuejian
Y
Wang
Xiaolian
X
Wu
Xiongfei
X
Wang
Shu
S
Chen
Xiaosong
X
Liu
D000818:Animals; D000906:Antibodies / Q000276:immunology; D005260:Female; D006108:Graphite / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D007114:Immunization; D007167:Immunotherapy; D007371:Interferon-gamma / Q000276:immunology; D053762:Interleukin-12 Subunit p40 / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D009369:Neoplasms / Q000276:immunology / Q000628:therapy; D053701:Receptors, Interleukin-10 / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000906:Antibodies; D053762:Interleukin-12 Subunit p40; D008070:Lipopolysaccharides; D053701:Receptors, Interleukin-10; D006108:Graphite; D007371:Interferon-gamma