Basic studies on radioimmunotargeting of CD133-positive HCT116 cancer stem cells.
As cancer stem cells (CSCs) are postulated to play critical roles in cancer development, including metastasis and recurrence, CSC imaging would provide valuable information for cancer treatment and lead to CSC-targeted therapy. To assess the possibility of in vivo CSC targeting, we conducted basic studies on radioimmunotargeting of cancer cells positive for CD133, a CSC marker recognized in various cancers. Antibodies against CD133 were labeled with ¹²⁵I, and their in vitro cell binding properties were tested. Using the same isotype IgG as a control, in vivo biodistribution of the labeled antibody retaining immunoreactivity was examined in mice bearing an HCT116 xenograft in which a population of the cancer cells expressed CD133. Intratumoral distribution of the labeled antibody was examined and compared to the CD133 expression pattern. The ¹²⁵I-labeled anti-CD133 antibody showed a modest but significantly higher accumulation in the HCT116 xenograft compared to the control IgG. The intratumoral distribution of the labeled antibody mostly overlapped with the CD133 expression, whereas the control IgG was found in the area close to the necrotic tumor center. Our results indicate that noninvasive in vivo targeting of CSCs could be possible with radiolabeled antibodies against cell membrane markers.
Molecular Imaging Center, National Institute of Radiological Sciences, Chiba, Japan.
Zhao-Hui
ZH
Jin
Chizuru
C
Sogawa
Takako
T
Furukawa
Yuriko
Y
Saito
Winn
W
Aung
Yasuhisa
Y
Fujibayashi
Tsuneo
T
Saga
D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D001345:Autoradiography; D045744:Cell Line, Tumor; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D018796:Immunoconjugates / Q000493:pharmacokinetics / Q000494:pharmacology*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014411:Neoplastic Stem Cells / Q000276:immunology / Q000528:radiation effects*; D010455:Peptides / Q000276:immunology*; D014018:Tissue Distribution
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; D018796:Immunoconjugates; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse