Targeted gene delivery via PEI complexed with an antibody.
Stable carriers are required for gene delivery. The use of polyethyleneimine (PEI) has been researched extensively; however, it is not suitable for targeted gene delivery to specific cells. To provide a targeting ability of the specific antibody to PEI, two repeats of Fc-binding domain of protein G (C2) were utilized. The constructed protein containing C2 could bind to a specific antibody and form a larger complex with plasmid DNA/PEI complex. The specific antibody to cell surface protein was bound to the complex through the use of C2 domain, and it was added to cells. As the result, the efficient expression of delivered reporter gene could be realized.
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
Joo Youn
JY
Bae
Masayasu
M
Mie
Eiry
E
Kobatake
D000906:Antibodies / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000737:chemistry; D004247:DNA / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D011094:Polyethyleneimine / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D000906:Antibodies; D001426:Bacterial Proteins; C034284:IgG Fc-binding protein, Streptococcus; D011993:Recombinant Fusion Proteins; D011094:Polyethyleneimine; D004247:DNA