ABO genotyping by TaqMan assay and allele frequencies in a Japanese population.
ABO genotyping have become common tools for forensic casework. We developed a new rapid ABO genotyping method using a fast real-time PCR system with the TaqMan® Sample-to-SNP™ Kit. Eight single nucleotide polymorphism (SNP) sites in the ABO gene (nt 261, 297, 467, 657, 703, 829, 930 and 1061) were selected to determine the ABO genotypes. ABO genotypes were easily determined by examining allelic discrimination patterns. This method enabled analyses to be completed in about 1h per plate with no postmortem change influences. The detection limit in each SNP site was examined as 100pg per reaction. ABO genotyping from 1000 Japanese individuals was also examined to determine the distribution of ABO genotypes and allele frequencies. Thus, 31 genotypes were clearly identified, and these were controlled by four common and seven rare alleles. The power of discrimination, heterozygosity and polymorphism information contents were 0.913, 0.775 and 0.812, respectively. Therefore, selecting these eight SNP sites could be useful for high specific ABO genotyping. This rapid, sensitive and accurate genotyping method is useful for forensic casework.
Department of Legal Medicine, Kitasato University School of Medicine, Kanagawa 252-0374, Japan. dm09027c@st.kitasato-u.ac.jp
Kazuho
K
Maeda
Shigeki
S
Nakamura
Chikako
C
Murakami
Wataru
W
Irie
Toshimasa
T
Watanabe
Chizuko
C
Sasaki
Naomi
N
Nakamaru
Masataka
M
Furukawa
Katsuyoshi
K
Kurihara
D000017:ABO Blood-Group System / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D017931:DNA Primers; D015342:DNA Probes; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007564:Japan; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation*
D000017:ABO Blood-Group System; D017931:DNA Primers; D015342:DNA Probes