New needle packed with polydimethylsiloxane having a micro-bore tunnel for headspace in-needle microextraction of aroma components of citrus oils.
A novel in-needle microextraction (INME) for headspace sampling evaluated in this study has significantly higher extraction speed and the practical merits of a durable stainless steel needle to overcome some exposed fiber related drawbacks. A prototype stainless steel needle (Hamilton 90022, 22 gauge bevel tip, 51 mm length) packed with polydimethylsiloxane (PDMS, 0.413 mm O.D., 10mm length) having a micro-bore (200 μm I.D.) tunnel was prepared as a new INME device. This needle with a barrel and a plunger is then inserted and exposed into the headspace over the sample. Headspace sampling can be speeded up by an automatic reciprocating pump. The extraction parameters have been optimized along with the validation of method performance. The methodology has been applied for the analysis of volatile aroma active components emitted from eight kinds of citrus essential oils by GC-FID or GC/MS. The proposed method showed excellent linearity, reproducibility, and low detection limit. This solventless technique is simple to operate, inexpensive to fabricate, and provides a facile means for collecting and introducing volatile aroma active components of essential oils.
Department of Chemistry, Seoul Women's University, Seoul, Republic of Korea.
Hyun-Hwa
HH
Son
Sunyoung
S
Bae
Dong-Sun
DS
Lee
D002957:Citrus / Q000737:chemistry*; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D004867:Equipment Design; D008401:Gas Chromatography-Mass Spectrometry; D009812:Odorants / Q000032:analysis*; D009822:Oils, Volatile / Q000032:analysis*; D010938:Plant Oils / Q000032:analysis*; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000295:instrumentation*
D004129:Dimethylpolysiloxanes; D009822:Oils, Volatile; D010938:Plant Oils