[Deuterium-depleted water selectively inhibits nasopharyngeal carcinoma cell proliferation in vitro].
OBJECTIVE
To evaluate the inhibitory effect of deuterium-depleted water (DDW) on the proliferation of nasopharyngeal carcinoma (NPC) cells in vitro and explore the possible mechanism.


METHODS
The growth inhibition of NPC cells and preosteoblast MC3T3-E1 cells following DDW treatment was measured by MTT assay and plate colony formation assay. The changes in migration and invasion of NPC cells were evaluated using Transwell and boyden chamber assays. The protein expression of proliferating cell nuclear antigen (PCNA) was determined using Western blotting. Flow cytometry was employed to evaluate the changes in cell cycle distribution after DDW treatment.


RESULTS
DDW with deuterium concentrations of 100, 75 and 50 ppm significantly suppressed the cell proliferation  (P<0.05) and lowered colony formation capacity and invasiveness of the NPC cells (P<0.01). Western blotting demonstrated a down-regulated expression of PCNA in the cells by DDW. DDW also caused obvious cell cycle arrest in the NPC cells with reduced cells in S phase and significantly increased cells in G(1) phase (P<0.05). Rather than causing growth inhibition, DDW promoted the growth of normal control MC3T3-E1 cells.


CONCLUSIONS
DDW possesses selective biological effects to inhibit the proliferation of NPC cells in vitro, suggesting the potential of DDW as a novel nontoxic adjuvant therapeutic agent in antitumor therapy.
Sino-America Cancer Research Institute, Guangdong Medical College, Dongguan, China. 403809283@qq.com
Hongqiang
H
Wang
Baohua
B
Zhu
Cong
C
Liu
Weiyi
W
Fang
Huiling
H
Yang
D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003903:Deuterium / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D014867:Water / Q000737:chemistry*
D018809:Proliferating Cell Nuclear Antigen; D014867:Water; D003903:Deuterium