Cell surface-associated proteins in the filamentous cyanobacterium Anabaena sp. strain PCC 7120.
The cell surface senses environmental changes first and transfers signals into the cell. To understand the response to environmental changes, it is necessary to analyze cell surface components, particularly cell surface-associated proteins. We therefore investigated cell surface-associated proteins from the filamentous cyanobacterium Anabaena sp. strain PCC 7120. The cell surface-associated proteins extracted by an acidic buffer were resolved by SDS-PAGE. Eighteen proteins were identified from resolved bands by amino-terminal sequencing. Analysis of cell surface-associated proteins indicated that several proteins among them were involved in nucleic acid binding, protein synthesis, proteolytic activity and electron transfer, and other proteins were involved in the stress response.
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3–8–1 Komaba, Meguro-ku, Tokyo 153–8902, Japan. yoshimura_h09@yahoo.co.jp
Hidehisa
H
Yoshimura
Masahiko
M
Ikeuchi
Masayuki
M
Ohomori
D017033:Anabaena / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D004579:Electron Transport / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics; D059748:Proteolysis; D013312:Stress, Physiological / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D008565:Membrane Proteins