Cutaneous respirometry by dynamic measurement of mitochondrial oxygen tension for monitoring mitochondrial function in vivo.
Progress in diagnosis and treatment of mitochondrial dysfunction in chronic and acute disease could greatly benefit from techniques for monitoring of mitochondrial function in vivo. In this study we demonstrate the feasibility of in vivo respirometry in skin. Mitochondrial oxygen measurements by means of oxygen-dependent delayed fluorescence of protoporphyrin IX are shown to provide a robust basis for measurement of local oxygen disappearance rate (ODR). The fundamental principles behind the technology are described, together with an analysis method for retrievel of respirometry data. The feasibility and reproducibility of this clinically useful approach are demonstrated in a series of rats.
Department of Anesthesiology, Laboratory of Experimental Anesthesiology, Erasmus MC - University Medical Center Rotterdam, 's-Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands.
Floor A
FA
Harms
Wilhelmina J
WJ
Voorbeijtel
Sander I A
SI
Bodmer
Nicolaas J H
NJ
Raat
Egbert G
EG
Mik
D000818:Animals; D019069:Cell Respiration; D008297:Male; D008928:Mitochondria / Q000737:chemistry* / Q000502:physiology*; D010100:Oxygen / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012867:Skin / Q000737:chemistry*
D010100:Oxygen