Three-dimensional paper-based electrochemiluminescence device for simultaneous detection of Pb2+ and Hg2+ based on potential-control technique.
In this work, a microfluidic paper-based analytical device (μPADs) has been proposed for simultaneous electrochemiluminescence (ECL) detection of lead ion (Pb(2+)) and mercury ion (Hg(2+)) based on oligonucleotide. The functionalized wax-patterned three-dimensional (3D) paper-based ECL device that can provide fast, cost-effective, simple, and sensitive detection for analysis was dependent on Pb(2+) and Hg(2+)-induced conformational change of DNA strands through the formation of G-quadruplex and T-Hg-T complex, respectively. The carbon nanocrystals (CNCs) capped silica nanoparticles (Si@CNCs) and Ru(bpy)(3)(2+)-gold nanoparticles (AuNPs) aggregates (Ru@AuNPs) were both used as ECL labels in our case. Structure characterization of Si@CNCs and Ru@AuNPs were obtained by the transmission electron microscope (TEM). Due to the different operational potentials of Si@CNCs and Ru@AuNPs, Pb(2+) and Hg(2+) coexisting in one paper working zone can be determined simultaneously with detection limits of 10 pM and 0.2 nM, respectively. Finally, this simple and cost-effective device was successfully applied for simultaneous detection of Pb(2+) and Hg(2+) in lake water and human serum samples, respectively.
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Meng
M
Zhang
Lei
L
Ge
Shenguang
S
Ge
Mei
M
Yan
Jinghua
J
Yu
Jiadong
J
Huang
Su
S
Liu
D015374:Biosensing Techniques / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D045424:Complex Mixtures / Q000032:analysis; D003217:Conductometry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007854:Lead / Q000032:analysis* / Q000097:blood; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation*; D008628:Mercury / Q000032:analysis* / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis*
D045424:Complex Mixtures; D014874:Water Pollutants, Chemical; D007854:Lead; D008628:Mercury