Identification and characterization of the chaetoviridin and chaetomugilin gene cluster in Chaetomium globosum reveal dual functions of an iterative highly-reducing polyketide synthase.
We report the identification and characterization of the caz biosynthetic cluster from  Chaetomium globosum  and the characterization of a highly reducing polyketide synthase (PKS) that acts in both a sequential and convergent manner with a nonreducing PKS to form the chaetomugilin and chaetoviridin azaphilones. Genetic inactivation studies verified the involvement of individual caz genes in the biosynthesis of the azaphilones. Through in vitro reconstitution, we demonstrated the in vitro synthesis of chaetoviridin A from the pyranoquinone intermediate cazisochromene using the highly reducing PKS and an acyltransferase.
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, California 90095, USA.
Jaclyn M
JM
Winter
Michio
M
Sato
Satoru
S
Sugimoto
Grace
G
Chiou
Neil K
NK
Garg
Yi
Y
Tang
Kenji
K
Watanabe
D002597:Chaetomium / Q000235:genetics*; D005663:Furans / Q000737:chemistry*; D015394:Molecular Structure; D048630:Polyketide Synthases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011753:Pyrones / Q000737:chemistry*
D005663:Furans; D011753:Pyrones; C579266:chaetomugilin M; C579267:chaetoviridin A; D048630:Polyketide Synthases