Purification of RNA expressed in vivo inserted in a tRNA scaffold.
For structural, biochemical, or pharmacological studies, it is required to have pure RNA in large quantities. In vitro transcription or chemical synthesis are the principal methods to produce RNA. Here, we describe an alternative method allowing RNA production in bacteria and its purification by liquid chromatography. In a few days, between 10 and 100 mg of pure RNA are obtained with this technique.
Laboratoire de Cristallographie et RMN biologiques, Université Paris Descartes, CNRS, Paris, France. luc.ponchon@parisdescartes.fr
Luc
L
Ponchon
Frédéric
F
Dardel
D002853:Chromatography, Liquid; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006515:Hepatitis B virus / Q000235:genetics; D006801:Humans; D012343:RNA, Transfer / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D012343:RNA, Transfer