Eco-friendly combination of the immobilized PGA enzyme and the S-Phacm protecting group for the synthesis of Cys-containing peptides.
Enzyme-labile protecting groups have emerged as a green alternative to conventional protecting groups. These groups introduce a further orthogonal dimension and eco-friendliness into protection schemes for the synthesis of complex polyfunctional organic molecules. S-Phacm, a Cys-protecting group, can be easily removed by the action of a covalently immobilized PGA enzyme under very mild conditions. Herein, the versatility and reliability of an eco-friendly combination of the immobilized PGA enzyme and the S-Phacm protecting group has been evaluated for the synthesis of diverse Cys-containing peptides.
Institute for Research in Biomedicine, 08028-Barcelona, Spain.
Miriam
M
Góngora-Benítez
Alessandra
A
Basso
Thomas
T
Bruckdorfer
Miriam
M
Royo
Judit
J
Tulla-Puche
Fernando
F
Albericio
D003545:Cysteine / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000138:chemical synthesis* / Q000737:chemistry*; D004926:Escherichia coli / Q000737:chemistry*; D055772:Green Chemistry Technology; D015394:Molecular Structure; D010399:Penicillin Amidase / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry*
D004800:Enzymes, Immobilized; D010455:Peptides; D010399:Penicillin Amidase; D003545:Cysteine