Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships.
To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma cells in the synovial membrane from patients with rheumatoid arthritis, using the combined approach.
Takashi
T
Sawai
Akihisa
A
Kamataki
Miwa
M
Uzuki
Kinji
K
Ishida
Tomohito
T
Hanasaka
Kensuke
K
Ochi
Takahito
T
Hashimoto
Takashi
T
Kubo
Akinari
A
Morikawa
Takahiro
T
Ochi
Koujiro
K
Tohyama
D001172:Arthritis, Rheumatoid / Q000473:pathology*; D002450:Cell Communication; D003713:Dendritic Cells / Q000502:physiology / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D008855:Microscopy, Electron, Scanning; D010950:Plasma Cells / Q000502:physiology / Q000648:ultrastructure*; D013583:Synovial Membrane / Q000648:ultrastructure*; D014057:Tomography, X-Ray Computed