Temporal changes in phosphatidylserine expression and glucose metabolism after myocardial infarction: an in vivo imaging study in mice.
Positron emission tomography (PET) for in vivo monitoring of phosphatidylserine externalization and glucose metabolism can potentially provide early predictors of outcome of cardioprotective therapies after myocardial infarction. We performed serial [⁶⁸Ga]annexin A5 PET (annexin-PET) and [¹⁸F]fluorodeoxyglucose PET (FDG-PET) after myocardial infarction to determine the time of peak phosphatidylserine externalization in relation to impaired glucose metabolism in infracted tissue. Annexin- and FDG-PET recordings were obtained in female (C57BL6/N) mice on days 1 to 4 after ligation of the left anterior descending (LAD) artery. [⁶⁸Ga]annexin A5 uptake (%ID/g) in the LAD artery territory increased from 1.7 ± 1.1 on day 1 to 5.0 ± 3.3 on day 2 and then declined to 2.0 ± 1.4 on day 3 (p  =  .047 vs day 2) and 1.6 ± 1.4 on day 4 (p  =  .014 vs day 2). These results matched apoptosis rates as estimated by autoradiography and fluorescein staining. FDG uptake (%ID/g) declined from 28 ± 14 on day 1 to 14 ± 3.5 on day 4 (p < .0001 vs day 1). Whereas FDG-PET revealed continuous loss of cell viability after permanent LAD artery occlusion, annexin-PET indicated peak phosphatidylserine expression at day 2, which might be the optimal time point for therapy monitoring.
Department of Nuclear Medicine, Institute of Veterinary Pathology, University of Munich, Munich, Germany.
Sebastian
S
Lehner
Andrei
A
Todica
Stefan
S
Brunner
Christopher
C
Uebleis
Hao
H
Wang
Carmen
C
Wängler
Nadja
N
Herbach
Tanja
T
Herrler
Guido
G
Böning
Rüdiger Paul
RP
Laubender
Paul
P
Cumming
Ralf
R
Schirrmacher
Wolfgang
W
Franz
Marcus
M
Hacker
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D001345:Autoradiography; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism; D005710:Gallium Radioisotopes / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000378:metabolism*; D010718:Phosphatidylserines / Q000378:metabolism*; D049268:Positron-Emission Tomography
D017304:Annexin A5; D005710:Gallium Radioisotopes; D010718:Phosphatidylserines; D019788:Fluorodeoxyglucose F18; D005947:Glucose