Construction of ratiometric fluorescent sensors by ribonucleopeptides.
Ratiometric fluorescent sensors were constructed from RNA aptamers by generating modular ribonucleopeptide complexes. Fluorescent ribonucleopeptides containing fluorophore seminaphthorhodafluor tethered to their peptide subunit revealed a dual emission property, which permitted a ratiometric fluorescent measurement of a substrate-binding event. The strategy successfully afforded ratiometric fluorescent sensors for biologically active small ligands, tetracycline, dopamine and streptomycin.
Dipartimento di Scienze Farmaceutiche Pietro Pratesi, Sezione Chimica Generale Organica A. Marchesini, Universita` degli Studi di Milano, via Venezian 21, 20133 Milan, Italy.
Chiara
C
Annoni
Eiji
E
Nakata
Tomoki
T
Tamura
Fong Fong
FF
Liew
Shun
S
Nakano
Maria Luisa
ML
Gelmi
Takashi
T
Morii
D000595:Amino Acid Sequence; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001578:Benzopyrans / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D004298:Dopamine / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D008969:Molecular Sequence Data; D009284:Naphthols / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D013307:Streptomycin / Q000032:analysis; D013752:Tetracycline / Q000032:analysis
D052157:Aptamers, Nucleotide; D001578:Benzopyrans; D005456:Fluorescent Dyes; D009284:Naphthols; D010455:Peptides; D012235:Rhodamines; C091666:seminaphthorhodaminefluoride; D013752:Tetracycline; D004298:Dopamine; D013307:Streptomycin