Phosphorylation of adenylyl cyclase III at serine1076 does not attenuate olfactory response in mice.
Feedback inhibition of adenylyl cyclase III (ACIII) via Ca(2+)-induced phosphorylation has long been hypothesized to contribute to response termination and adaptation of olfactory sensory neurons (OSNs). To directly determine the functional significance of this feedback mechanism for olfaction in vivo, we genetically mutated serine(1076) of ACIII, the only residue responsible for Ca(2+)-induced phosphorylation and inhibition of ACIII (Wei et al., 1996, 1998), to alanine in mice. Immunohistochemistry and Western blot analysis showed that the mutation affects neither the cilial localization nor the expression level of ACIII in OSNs. Electroolfactogram analysis showed no differences in the responses between wild-type and mutant mice to single-pulse odorant stimulations or in several stimulation paradigms for adaptation. These results suggest that phosphorylation of ACIII on serine(1076) plays a far less important role in olfactory response attenuation than previously thought.
Biology Department, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Katherine D
KD
Cygnar
Sarah Ellen
SE
Collins
Christopher H
CH
Ferguson
Chantal
C
Bodkin-Clarke
Haiqing
H
Zhao
D000262:Adenylyl Cyclases / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D009832:Olfactory Nerve / Q000201:enzymology* / Q000378:metabolism; D010766:Phosphorylation / Q000235:genetics; D012694:Serine / Q000235:genetics*; D012903:Smell / Q000235:genetics*
D012694:Serine; D000262:Adenylyl Cyclases; C421784:adenylate cyclase 3