Effect of microtopographic structures of silk fibroin on endothelial cell behavior.
Stent implantation has become the preferred revascularization treatment for occlusive blood vessel disease; however, there are occasionally complications resulting in re-narrowing of the treated artery. One approach to overcoming this problem is to establish a confluent monolayer of endothelial cells (ECs) on the stent, and a coating would facilitate the attachment of ECs. Silk fibroin was reported to be used as an ideal coating applied to stent for the culture of human ECs. The aim of the present study is to gain more insight into the influence of the internal microtopographical structure of silk fibroin on cell behavior, such as attachment and growth, and to further investigate its molecular mechanism using human umbilical vein ECs (HUVECs). Our results evaluated the effect of different microtopographical structures on cell behavior. In addition, we analyzed the cell cycle and investigated relevant molecules involved. The results indicated that the microtopographic structure of silk fibroin was associated with EC morphology, attachment and proliferation.
Department of Surgery, Huashan Hospital of Fudan University, Shanghai 200040, PR China.
Jin-Yun
JY
Tan
Jian-Chuan
JC
Wen
Wei-Hao
WH
Shi
Qing
Q
He
Lei
L
Zhu
Kun
K
Liang
Zheng-Zhong
ZZ
Shao
Bo
B
Yu
D001672:Biocompatible Materials; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002453:Cell Cycle; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D005349:Fibroins / Q000737:chemistry* / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering
D001672:Biocompatible Materials; D047011:Silk; D005349:Fibroins