Development of an immunochromatographic strip for the detection of antibodies against Porcine circovirus-2.
A rapid (<5 min) immunochromatographic strip using a colloidal gold-labeled antigen probe was successfully developed and applied for the detection of Porcine circovirus-2 (PCV-2) antibodies in swine. Recombinant Cap protein truncated nuclear localization signal of PCV-2, was expressed and labeled with colloidal gold. This conjugate was dispensed on a conjugate pad as the detector. Staphylococcal protein A and purified porcine anti-PCV-2 antibodies were blotted on a nitrocellulose membrane for the test and control lines, respectively. Sensitivity and specificity of this strip test was evaluated using PCV-2 antisera as well as other sera from pigs infected with a variety of swine viruses. For the validation of this strip test, 500 clinical swine serum samples were assessed both by the strip and a commercial enzyme-linked immunosorbent assay (ELISA) kit. The agreement between the immunochromatographic strip and ELISA kit was 94.00%. This strip possesses high sensitivity and specificity and may be useful as a candidate for rapid diagnosis of PCV-2 antibodies in the field.
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, People’s Republic of China.
Qianyue
Q
Jin
Jifei
J
Yang
Qingxia
Q
Lu
Junqing
J
Guo
Ruiguang
R
Deng
Yinbiao
Y
Wang
Shihong
S
Wang
Shufen
S
Wang
Wen
W
Chen
Yubao
Y
Zhi
Li
L
Wang
Suzhen
S
Yang
Gaiping
G
Zhang
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism; D002846:Chromatography, Affinity / Q000662:veterinary*; D018173:Circoviridae Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D017925:Circovirus / Q000145:classification* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D011934:Reagent Strips; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000097:blood*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D011934:Reagent Strips; D011994:Recombinant Proteins