Development and characterization of DNA microsatellite primers for buriti (Mauritia flexuosa L.f.).
Mauritia flexuosa L. (Arecaceae) is a palm tree species known as buriti  that occurs in the Cerrado biome. It is characteristic of the vereda, a typical  ecosystem of central Brazil. In this phytophysiognomy, M. flexuosa and  other groups of arboreal-herbaceous species develop in open fields with very  humid soils. M. flexuosa can be found in forest borders and is a palm  tree with a wide distribution in South America (Brazil, Colombia, Venezuela,  French Guyana Ecuador, Peru, and Bolivia). The main objectives of this study  were to develop simple sequence repeat marker-enriched libraries and to  characterize these loci in buriti palm to facilitate future population studies.  A total of 40 sequences derived from the microsatellite-enriched libraries were  selected for primer design. The optimization results showed that 9 primer pairs  could successfully amplify polymorphic target fragments of the expected sizes.  The data also show that the described primers can be used in population genetic  studies in M. flexuosa to obtain information that will inform  conservation and management strategies.
Laboratório de Genética da Conservação, Universidade Estadual de Montes Claros, Campus Universitário Professor Darcy Ribeiro, Montes Claros, MG, Brasil.
E V
EV
Menezes
W F S
WF
Souto
A Y
AY
Ciampi
V C R
VC
Azevedo
H M
HM
Valério
M A S
MA
Pimenta
D000483:Alleles; D028023:Arecaceae / Q000235:genetics*; D017931:DNA Primers / Q000378:metabolism*; D018744:DNA, Plant / Q000235:genetics*; D056426:Genetic Loci / Q000235:genetics; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*; D059372:Nucleotide Motifs / Q000235:genetics; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics
D017931:DNA Primers; D018744:DNA, Plant