Effect of skimmed pasteurized milk and Hank's balanced salt solution on viability and osteogenic differentiation of human periodontal ligament stem cells.
OBJECTIVE
The purpose of this study was to compare the effect of skimmed pasteurized milk and Hank's balanced salt solution on the viability and osteogenic differentiation potential of the human periodontal ligament stem cells at room temperature in vitro.


METHODS
Human periodontal ligament stem cells were obtained from extracted healthy third molars and conserved in skimmed pasteurized milk and Hank's balanced salt solution for 1, 2, and 4 h at room temperature to detect the viability of the cells and their osteogenic differentiation potential.


RESULTS
The efficacy of skimmed pasteurized milk on cell viability at 4 h was significantly higher than that of HBSS (P < 0.05), and cells stored in skimmed pasteurized milk showed significantly higher levels of mineralization than those in HBSS at 2 and 4 h (P < 0.05).


CONCLUSIONS
Skimmed pasteurized milk was more effective than Hank's balanced salt solution in maintaining the viability and osteogenic differentiation potential of PDLSCs at room temperature in vitro.
Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
Wen-Jun
WJ
Wang
Yu-Ming
YM
Zhao
Xiao-Yu
XY
Feng
Wei-Qian
WQ
Jia
Li-Hong
LH
Ge
D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D017931:DNA Primers; D006801:Humans; D007552:Isotonic Solutions; D008892:Milk; D010012:Osteogenesis; D010513:Periodontal Ligament / Q000166:cytology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012844:Sincalide / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D017931:DNA Primers; C072670:Hanks Balanced Salt Solution; D007552:Isotonic Solutions; D012844:Sincalide