Effect of cobalt(II) complexes with dipyridylamine and salicylaldehydes on cultured tumor and non-tumor cells: synthesis, crystal structure investigations and biological activity.
The synthesis of eight mixed-ligand cobalt(II) complexes with 2,2'-dipyridylamine (dpamH) and substituted salicylaldehydes (X-saloH) was undertaken in an effort to discover new compounds with anticancer activity. The complexes with the general formula [Co(dpamH)(2)(X-salo)]Y, (Y=Br or Cl) were characterized by elemental analyses, FT-IR and UV-visible spectroscopy, magnetic and conductivity measurements. The structures of two of them [Co(dpamH)(2)(5-CH(3)-salo)]Br and [Co(dpamH)(2)(3-OCH(3)-salo)]Cl, as well as of the precursors [Co(dpamH)(3)]Br(2) and [Co(dpamH)(2)Cl(H(2)O)]Cl, were determined by X-ray crystallography revealing octahedral coordination of cobalt(II) and mononuclear complexes. The complexes were thermally stable up to 200 °C in nitrogen atmosphere, studied by simultaneous TG/DTG-DTA technique. The two precursor Co compounds, as well as four of the title compounds, were evaluated for their efficacy as anticancer agents against different cancer and normal human cell lines. The in vitro chemosensitivity of various human cell lines to these Co complexes was evaluated by measuring cell growth inhibition by employing the SRB colorimetric assay. A series of experiments showed a dose-dependent cytotoxic activity of the complexes against all cell lines used. These findings represent a prompting to search for possible interaction of these complexes with other cellular elements of fundamental importance in cell proliferation.
Aristotle University of Thessaloniki, Department of Chemistry, Laboratory of Inorganic Chemistry, Thessaloniki 54 124, Greece. lalia@chem.auth.gr
Maria
M
Lalia-Kantouri
Maria
M
Gdaniec
Theodora
T
Choli-Papadopoulou
Andreas
A
Badounas
Christos D
CD
Papadopoulos
Agnieszka
A
Czapik
George D
GD
Geromichalos
Despina
D
Sahpazidou
Fani
F
Tsitouroudi
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000447:Aldehydes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003035:Cobalt / Q000737:chemistry*; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D008024:Ligands
D000447:Aldehydes; D000970:Antineoplastic Agents; D056831:Coordination Complexes; D008024:Ligands; C013243:salicylaldehyde; D003035:Cobalt; D015082:2,2'-Dipyridyl; C048062:2,2'-dipyridylamine