Expression and localization of silkworm adaptor protein complex-1 subunits, which were down-regulated post baculovirus infection.
Adaptor protein complexes (APs) function as vesicle coat components in different membrane traffic pathways. In this study the subunits of adaptor protein complex-1 (AP-1) of silkworm Bombyx mori were molecularly characterized. All coding genes for the four subunits were cloned and sequenced. Phylogenic tree for each adaptin was constructed and all subunits were found to be conserved in respective group among organisms. The mRNA expression pattern for each adaptin was similar among tissues. Alternative splicing event was observed in genes encoding both the heavy chain gamma and beta adaptin and the light chain subunit, which could generate other possible adaptin forms. GFP-tagged fusion proteins indicated that AP-1 located in the peripheral plasma area. Furthermore, the BmNPV infection in B. mori cells had differentiated effect on the expression level of AP-1 subunits.
Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Yan-Shan
YS
Niu
Mei-Xian
MX
Wang
Shuang
S
Liang
Fang
F
Zhou
Yun-Gen
YG
Miao
D034001:Adaptor Protein Complex Subunits / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D016367:Baculoviridae / Q000502:physiology*; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D002460:Cell Line; D003001:Cloning, Molecular; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014777:Virus Diseases / Q000235:genetics*
D034001:Adaptor Protein Complex Subunits; D020033:Protein Isoforms; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins