Combining bulk segregation analysis and microarrays for mapping of the pH trait in melon.
The availability of sequence information for many plants has opened the way to advanced genetic analysis in many non-model plants. Nevertheless, exploration of genetic variation on a large scale and its use as a tool for the identification of traits of interest are still rare. In this study, we combined a bulk segregation approach with our own-designed microarrays to map the pH locus that influences fruit pH in melon. Using these technologies, we identified a set of markers that are genetically linked to the pH trait. Further analysis using a set of melon cultivars demonstrated that some of these markers are tightly linked to the pH trait throughout our germplasm collection. These results validate the utility of combining microarray technology with a bulk segregation approach in mapping traits of interest in non-model plants.
Genomic Unit, Institute of Plant Sciences, Volcani Research Center, Agricultural Research Organization, 50250 Bet Dagan, Israel. asherman@agri.gov.il
Amir
A
Sherman
Ravit
R
Eshed
Rotem
R
Harel-Beja
Galil
G
Tzuri
Vitaly
V
Portnoy
Shahar
S
Cohen
Mor
M
Rubinstein
Arthur A
AA
Schaffer
Joseph
J
Burger
Nurit
N
Katzir
Ron
R
Ophir
D015415:Biomarkers / Q000378:metabolism*; D002874:Chromosome Mapping; D020090:Chromosome Segregation; D019662:Cucurbitaceae / Q000235:genetics*; D020869:Gene Expression Profiling; D017343:Genes, Plant / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D015810:Linkage Disequilibrium; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D040641:Quantitative Trait Loci
D015415:Biomarkers