Effect of electroacupuncture on the expression of mTOR and eIF4E in hippocampus of rats with vascular dementia.
Clinically, electroacupuncture is proved to be an effective therapy for vascular dementia; however, their mechanisms remain uncertain. The aim of the current study was to investigate the mechanism of electroacupuncture therapy for vascular dementia. One month after a vascular dementia animal model was established by bilateral occlusion of common carotid arteries, electroacupuncture treatment was given at "Baihui" (DU20), "Dazhui" (DU14), and "Shenshu" (BL23). Morris water maze was used to assess the learning and memory ability of rats. Western blot assay was performed to detect the expression of mammalian target of rapamycin (mTOR) and eukaryotic translation initiation factor 4E (eIF4E) in hippocampus of rats. Morris water maze test showed that electroacupuncture improved the learning ability of vascular dementia rats. Western blot assay revealed that the expression level of mTOR and eIF4E in the electroacupuncture group and sham-operated group was higher than that in the vascular dementia group (P < 0.05). In conclusion, the decreasing expression of mTOR and eIF4E plays important roles in the pathogenesis of vascular dementia. Electroacupuncture improves learning and memory ability by up-regulating expression of mTOR and eIF4E in the hippocampus of vascular dementia rats.
Department of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fujian 350122, China.
Yanzhen
Y
Zhu
Yanjun
Y
Zeng
Xuan
X
Wang
Xiaobao
X
Ye
D000818:Animals; D015140:Dementia, Vascular / Q000378:metabolism* / Q000628:therapy*; D015671:Electroacupuncture / Q000379:methods*; D039561:Eukaryotic Initiation Factor-4E / Q000096:biosynthesis*; D005260:Female; D006624:Hippocampus / Q000378:metabolism*; D018782:Maze Learning / Q000502:physiology; D008568:Memory / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D058570:TOR Serine-Threonine Kinases / Q000096:biosynthesis*; D016896:Treatment Outcome
D039561:Eukaryotic Initiation Factor-4E; C546843:mTOR protein, mouse; D058570:TOR Serine-Threonine Kinases