Curcumin inhibits LPS-induced EMT through downregulation of NF-κB-Snail             signaling in breast cancer cells.
Epithelial-mesenchymal transition (EMT) is considered a critical event in             cancer cell invasion and metastasis. Emerging evidence has shown that curcumin             may prevent or delay the progression of cancer, an effect that may be partially             due to its ability to disrupt EMT, yet this has not yet been demonstrated. In             this study, we used lipopolysaccharide (LPS) to trigger EMT in MCF-7 and MDA-MB-231             breast cancer cell lines and showed that curcumin inhibited LPS-induced morphological             changes, decreased the expression of LPS-induced markers of EMT such as vimentin,             and increased the expression of E-cadherin, resulting in the inhibition of in vitro             cell motility and invasiveness. We discovered that these actions were mediated             through the inactivation of NF-κB-Snail signaling pathways. Our results indicate             that curcumin plays an important role in the inhibition of LPS-induced EMT in             breast cancer cells through the downregulation of NF-κB-Snail activity. These             data provide a new perspective of the anti-invasive mechanism of curcumin, indicating             that the effect is partly due to its ability to attack the EMT process.
Department of Orthopedic Surgery, Affiliated Hospital of Xi'an Medical College, Xi'an 710077, PR China.
Tao
T
Huang
Zhijun
Z
Chen
Liping
L
Fang
D000970:Antineoplastic Agents / Q000494:pharmacology; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D003474:Curcumin / Q000494:pharmacology*; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors; D003474:Curcumin