Subgingival microflora in inflammatory bowel disease patients with untreated periodontitis.
OBJECTIVE
To analyze the subgingival microflora composition of inflammatory bowel disease (IBD) patients with untreated chronic periodontitis and compare them with systemically healthy controls also having untreated chronic periodontitis.


METHODS
Thirty IBD patients [15 with Crohn's disease (CD) and 15 with ulcerative colitis (UC)] and 15 control individuals participated in the study. All patients had been diagnosed with untreated chronic periodontitis. From every patient, subgingival plaque was collected from four gingivitis and four periodontitis sites with paper points. Samples from the same category (gingivitis or periodontitis) in each patient were pooled together and stored at -70 °C until analysis using a checkerboard DNA-DNA hybridization technique for 74 bacterial species.


RESULTS
Multiple-comparison analysis showed that the groups differed in bacterial counts for Bacteroides ureolyticus, Campylobacter gracilis, Parvimonas micra, Prevotella melaninogenica, Peptostreptococcus anaerobius, Staphylococcus aureus, Streptococcus anginosus, Streptococcus intermedius, Streptococcus mitis, Streptococcus mutans, and Treponema denticola (P<0.001). CD patients had significantly higher levels of these bacteria than UC patients either in gingivitis or in periodontitis sites (P<0.05). CD patients harbored higher levels of P. melaninogenica, S. aureus, S. anginosus, and S. mutans compared with controls both at gingivitis and at periodontitis sites (P<0.05). UC patients harbored higher levels of S. aureus (P=0.01) and P. anaerobius (P=0.05) than controls only in gingivitis sites.


CONCLUSIONS
Our study showed that even with similar clinical periodontal parameters, IBD patients harbor higher levels of bacteria that are related to opportunistic infections in inflamed subgingival sites that might be harmful for the crucial microbe-host interaction.
Departament of Periodontology, Faculty of Odontology, Rio de Janeiro State University, Vila Isabel, Rio de Janeiro, Brazil.
Fernanda
F
Brito
Cyrla
C
Zaltman
Ana T P
AT
Carvalho
Ricardo G
RG
Fischer
Rutger
R
Persson
Anders
A
Gustafsson
Carlos M S
CM
Figueredo
D000328:Adult; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000150:complications / Q000382:microbiology*; D003093:Colitis, Ulcerative / Q000150:complications / Q000382:microbiology; D003424:Crohn Disease / Q000150:complications / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005891:Gingivitis / Q000150:complications / Q000382:microbiology*; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000150:complications / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009894:Opportunistic Infections / Q000150:complications / Q000382:microbiology
D004269:DNA, Bacterial