Fate of caffeine in mesocosms wetland planted with Scirpus validus.
Uptake, accumulation and translocation of caffeine by Scirpus validus grown in hydroponic condition were investigated. The plants were cultivated in Hoagland's nutrient solution spiked with caffeine at concentrations of 0.5-2.0 mg L(-1). The effect of photodegradation on caffeine elimination was determined in dark controls and proved to be negligible. Removal of caffeine in mesocosms without plants showed however that biodegradation could account for about 15-19% of the caffeine lost from solutions after 3 and 7 d. Plant uptake played a significant role in caffeine elimination. Caffeine was detected in both roots and shoots of S. validus. Root concentrations of caffeine were 0.1-6.1 μg g(-1), while the concentrations for shoots were 6.4-13.7 μg g(-1). A significant (p<0.05) positive correlation between the concentration in the root and the initial concentrations in the nutrient solution was observed. The bioaccumulation factors (BAFs) of caffeine for roots ranged from 0.2 to 3.1, while BAFs for shoots ranged from 3.2 to 16.9. Translocation from roots to shoots was the major pathway of shoot accumulation. The fraction of caffeine in the roots as a percentage of the total caffeine mass in solution was limited to 0.2-4.4% throughout the whole experiment, while shoot uptake percentage ranged from 12% to 25% for caffeine at the initial concentration of 2.0 mg L(-1) to 50-62% for caffeine at the initial concentration of 0.5 mg L(-1). However, a marked decrease in the concentration of caffeine in the shoots between d-14 and d-21 suggests that caffeine may have been catabolized in the plant tissues subsequent to plant uptake and translocation.
DHI-NTU Centre, Nanyang Environment & Water Research Institute, N1.2-B1-02, Nanyang Technological University, Singapore 639798, Singapore. dqzhang@ntu.edu.sg
Dong Qing
DQ
Zhang
Tao
T
Hua
Richard M
RM
Gersberg
Junfei
J
Zhu
Wun Jern
WJ
Ng
Soon Keat
SK
Tan
D001673:Biodegradation, Environmental; D002110:Caffeine / Q000032:analysis* / Q000378:metabolism; D029785:Cyperaceae / Q000254:growth & development / Q000378:metabolism*; D018527:Hydroponics; D008956:Models, Chemical; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism; D053833:Wetlands
D014874:Water Pollutants, Chemical; D002110:Caffeine