[mRNA transcription of translation elongation factor and primase in different segments of Moniezia expansa].
A SYBR green real-time fluorescent quantitative PCR assay was developed for detection of Moniezia expansa mRNA with its beta-tubulin as an internal control. The results showed a good linear relationship (>0.99) between the Ct value and the concentration of positive plasmid for each gene from scolex and various proglottids. Real-time PCR showed that the expression abundance of translation elongation factor and primase was different. In conclusion, the transcription level of translation elongation factor and primase was high in both scolex and immature segment, suggesting that they may play a role in the development of scolex and immature segment.
Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China.
Zheng-Rong
ZR
Wang
Xin-Wen
XW
Bo
Wen-Juan
WJ
Zhao
Li-Chao
LC
Kang
D000818:Animals; D002589:Cestoda / Q000235:genetics*; D019915:DNA Primase / Q000235:genetics*; D010445:Peptide Elongation Factors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D010445:Peptide Elongation Factors; D012333:RNA, Messenger; D019915:DNA Primase