MUC-1 aptamer-conjugated dye-doped silica nanoparticles for MCF-7 cells detection.
In this work, we have prepared three types of aptamer-conjugated Rubpy-doped silica nanoparticles for Human breast carcinoma MCF-7 cells labeling. Probe A is prepared through covalent conjugation between amine-labeled MUC-1 aptamer and carboxyl-modified Rubpy-doped NPs (NPs-aptamer). Probe B is prepared based on the interaction between biotin-labeled MUC-1 aptamer and avidin-conjugated Rubpy-doped NPs (NPs-avidin-biotin-aptamer). For Probe C, there is a PEG with flexible long chain as the bridge between avidin and the NPs (NPs-PEG-avidin-biotin-aptamer). In addition, we further investigate the practical number of MUC-1 aptamers on an NP of each probe using hoechst33258 dye. The binding efficiency of MUC-1 aptamer on the three types of probes as follows: Probe A < Probe B < Probe C. In addition, microscopic fluorescence imaging shows that Probe C containing the PEG molecules can be effectively applied for the recognition of MUC-1 protein in human breast carcinoma MCF-7 cells thus demonstrates that the PEG with flexible long chain as the bridge between the aptamer and NP can greatly enhances the freedom of MUC-1 aptamer. Compared with common organic dyes, the dye-doped silica nanoparticles serve as a stable bioprobe because of their facile conjugation with the desirable biomolecules, and have exhibited great potential in bioanalysis.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
Li
L
Cai
Ze-Zhong
ZZ
Chen
Min-Yan
MY
Chen
Hong-Wu
HW
Tang
Dai-Wen
DW
Pang
D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000378:metabolism; D001943:Breast Neoplasms / Q000175:diagnosis*; D004396:Coloring Agents / Q000737:chemistry*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells / Q000378:metabolism* / Q000473:pathology; D018396:Mucin-1 / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry*; D011485:Protein Binding; D012822:Silicon Dioxide / Q000737:chemistry*
D052157:Aptamers, Nucleotide; D004396:Coloring Agents; D018396:Mucin-1; D012822:Silicon Dioxide